gucy2g cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gucy2g
Entrez Gene ID	100333350
Full Name	guanylate cyclase 2g
Synonyms	si:ch73-92e20.2
General protein information	Preferred Names guanylate cyclase 2g Names guanylate cyclase 2G-like zmp:0000000809
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002663981.5	XP_002664027.2	guanylate cyclase 2G-like isoform X2
XM_009306792.2	XP_009305067.2	guanylate cyclase 2G-like isoform X1
XM_009306793.2	XP_009305068.1	guanylate cyclase 2G-like isoform X3
XM_009306792.3	XP_009305067.2	guanylate cyclase 2G-like isoform X1
XM_002663981.6	XP_002664027.2	guanylate cyclase 2G-like isoform X2
XM_009306793.3	XP_009305068.1	guanylate cyclase 2G-like isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC100688545	XP_005637936.1
Rattus norvegicus (Norway rat)	Gucy2g	NP_620611.2
Danio rerio (zebrafish)	si:ch73-92e20.2	XP_002664027.2
Pan troglodytes (chimpanzee)	LOC466203	XP_003312856.1
Mus musculus (house mouse)	Gucy2g	NP_001074545.1

Gene Ontology

The following gucy2g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gucy2g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa54524	XM_009306792.3 Latest version!	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa18041	XM_002663981.6 Latest version!	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa49811	XM_009306793.3 Latest version!	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa54524	XM_009306792.2	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa18041	XM_002663981.5	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa49811	XM_009306793.2	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gucy2g ( XM_009306792.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305067.2 Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54524
Clone ID Related Accession (Same CDS sequence)	XM_009306792.2 , XM_009306792.3
Accession Version	XM_009306792.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	guanylate cyclase 2G-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAG GATTAGCCTC AGACACAAGT CGACCCATAA GATGGTGGCT GCTGTTTACA 
GCTTTTACAA ACTCTGTCAT TTGCAACAAA GACGGCCAGT TCAAGCTAAC GGTGGGCCTT 
CAGGCTCCTC GGAACATTTC TTACCCTTTC AGTTTGCTCA GGCTGGGGTC AGCGATGCAA 
ATAGCCATGG ACAAGATCAA CTCAAATCCA GCTCTCAGTG GAAACTTCAC CTTTGACTTC 
GTCTACATGG ACACAGACTG TAACGCCAAA ACCTCACTGA GGGGTTTCAT CGAGCAAGTA 
ATGGAGCAAA ATGTGTCCGC GTTATTTGGT CCCCCCTGTC CTGAGGAGGC AGAAGTCACT 
GGGCTCATTG CATCCACTTG GAACATTCCC ATGTTCGGAT TTGTTGGTCA GACACCAAAA 
ATGGATAACA TTCAGGTGTA CGACACCTAT ATAAAAATTG TACCTCCCCT TAAAAGGGTG 
GGAGAGATCC TTCTTAAAGC ATTGGAATTT TTCGGATGGA AGCATGTTGG CATGATCGGA 
GGCGGAGCGG ATGCAAATAC GTGGGACAGT GTGGAGGCCC TTTGGAAGTC TGTGGATCAG 
CAACTCCGAA GCAAAGTAAC AGTGACGATG GGCATTAAGT TTGACACCAG TGACCTGGAA 
CAGGCAAAGA GGAACTTGCA AATCATCTCT AAGGTTGCTA GAGTGGTGGT TGTGCTTGCT 
AATGCTGAGG ACTCCACAGC TATGATGATA GAAGCTCAGA AACTGGGCCT GATGAGTGGA 
GAGTATGTTT TCCTTCTGGT TCAGCAGTTT GAGGTCAGTG GCAAAGTGGA CAACCTGTGG 
AAGACTTCTG TGAGCGAAAC AAATAGAACA ATGCTGAAGG CTTTTGACAT GGTGTTTGTA 
CTAGCTGAGA AGTCCTATGA TGGATATGAC TACTATGACT TCTTCGATCA GGCATATGAT 
AAACTGAAAG GAGATCCTTT TTACAGCAAC CTGTCATCAG TAAAAGAAGT AAGCTCATAT 
GCTGCCTATT TACATGATGC TGTCTTACTT TATGCCATGG GCCTCAAAGA AGCATTTAAA 
AGTGGCAAAG ACATTCGTCA TGGACGAGAA ATTGTCCAAA AACTCAGGGA CAGGACAAGC 
ATTAGATTTT ATGGAGCTTC GGGTTTGGTT CACTTTGATG AATATGGAGA GAGGAACCTA 
GATTATTCAG TATATGACCT GCAACAAACA GAAACTTTAA TACAATTTGT CTCTGTTTTG 
GATTTTGACA GCCATACGAA AATGATCAAA CCCACACTAA GGTTTTCGAA TATAGTTTGG 
CAAAACGGCA AACCCCCTAC AGATAATCCA AGCTGTGGTT TTGATAATGA ACTCTGTGAA 
TGGCTAGACA ATGAGATTTC TCTTCTGGTT CTGCTGGTGG CTTTGCCACT GATTGGAGTG 
GCCGCAGTTT CTTTGATTAC TTTGTTGACT CTGCAGAAGC TGCGCTTACA AAGCCGCCTG 
GATGAATCCA ACTGGTGGCT CATCAACTAC AGTGATATAA CTATCCTCAG AGAGCCCAAA 
GGAACACAAA TCCTGTCTGT AAACACAACA CCAAGCAAAT GTGGCAGTGG TGGCTCTCAG 
TCCATGCTCT CCTCCAACAG CTGTAACACT AGAGAAGCCA TCTACGCTAC CGTCGGACTC 
TTTCAGGGAA ACGAGGTGGG CATAAAGTAT TTGAAAAATC AAATCATCCC TGATATCAAG 
AAACCTTCGA TCATCGCGGA GTTCAATATG CTGAAAGAAA TGAAGCATGA GAACCTGGTA 
CAATTTTTCG GTGTATGTAT TGATCCACCA AATGTCTGTA TCGTCATGCA GTATTGCAAG 
AAAGGGAGCT TGAAGGATGT TCTTGGAAAC CATGAAATTG ATCTGGACTG GATGTTTAAA 
CTGTCCTTTG CGTATGACAT TGTTAATGGC ATGGAGTACA TCCATAAGAG CAGTCTGAAG 
TCTCATGGAA ACCTGAGGCC CAGCACGTGT TTGGTGGACA GCAGGCTTCA GATCAAACTC 
TCCGGCTTTG GCCTGTGGGA GTTCAAATGT GGCACCAAAC ACAGACTGAT TCCGTTGGAC 
AGTCCAAAAT ATGAAGAGAT GTACTGGACG GCTCCTGAGT TTTTGAGAGA GATCTTCTAC 
CCTTTCAATG GCACCCAGAA GGGAGATGTC TATAGCTTTG CCATAATCAT CCGAGAGCTC 
ATATACAGTA CAGAGGTGGG CCCGTATCAT GACGTTCATC TGGAACCAAA AGAAATCATT 
AAGCAGTTAA GGACCCCTAT GAGTGAGGAG CCTCTTAGAC CCACCCTGTC TGCTGACATC 
TGTGACGAAC GTCTCATCCC GTTAATTAAA GCCTGCTGGA GTGAAAACCC AGACCACAGA 
CCCCCGTTCG GCAGTATCAG AAGACAACTG AGAGACGCTT GTCCTGAAAG CCATGCCAAC 
ATCCTGGACA ACATGGTGAA CAAGCTGGAG AAATATGCCA ATCATCTGGA AGAAGTGGTG 
GAGGAGAGAA CCAGTCAGCT GACCGTGGAG AAGAGCAGAG CTGATAAGCT TCTCTCTAGC 
ATGCTGCCCA GGTATATTGC TGATCAGCTA ATGGCTGGAA AATCAGTGGA GCCCAGAAGT 
TATGAAATGG TAACCATCTT CTTCTCTGAC ATTGTGGGTT TCACCACTAT GTGCTCTGTT 
AGCTCTGCTC TGGAGGTGGT CACTCTTCTC AATGACCTCT ACAGCCTTTT TGATGATATC 
ATCAAGCTAT ATGATGTCTA CAAGGTGGAG ACTATTGGAG ATGCCTATAT GGTGGCCAGT 
GGACTTCCTA TCAGTAATGG TACTCTTCAT GCTGAGGAGA TCTCTACTAT GGCATTGCAC 
TTCCTCTCTT CCATCAAAAG GTTCAAAATA AGGCACCTGC CCAATGAGAG ACTAGCCCTA 
CGGATTGGGA TTAATTCTGG CCCAGTAGTT GCTGGAGTAG TGGGTAGTAC AATGCCTCGC 
TACTGTTTAT TTGGAGATAC AGTCAACACA GCTTCTAGGA TGGAGAGCAA TAGTCTTCCA 
CTGAAAATCC ATATCTCTCA GAGCACTGCT GATATTCTCT TGACAATTGG CACATTTGAA 
CTGGAGGAAA GGGGCGATAT TGAAATTAAG GGAAAGGGAA CACAGAAAAC GTTCTGGTTG 
TTGAGCAAAC CTGGATTCAC GTTTCCACCC ACTGGCCAGG ACAGTAATCC AAGTCCTAAA 
TCAGGAACAG ATTCTTGTCA CATCAAACAG GAGAAAACCA AAGCAGCAAA TGATGGAGAT 
TTTAAAGAGA GAAGAACAAT ACAGCGGACA TCGGCAATGA CTAAAATGAC AGACATGTAC 
ACCCTCACTG TACCTGATGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305067.2
CDS	208..3591
Translation	MSGGLASDTSRPIRWWLLFTAFTNSVICNKDGQFKLTVGLQAPRNISYPFSLLRLGSAMQIAMDKINSNPALSGNFTFDFVYMDTDCNAKTSLRGFIEQVMEQNVSALFGPPCPEEAEVTGLIASTWNIPMFGFVGQTPKMDNIQVYDTYIKIVPPLKRVGEILLKALEFFGWKHVGMIGGGADANTWDSVEALWKSVDQQLRSKVTVTMGIKFDTSDLEQAKRNLQIISKVARVVVVLANAEDSTAMMIEAQKLGLMSGEYVFLLVQQFEVSGKVDNLWKTSVSETNRTMLKAFDMVFVLAEKSYDGYDYYDFFDQAYDKLKGDPFYSNLSSVKEVSSYAAYLHDAVLLYAMGLKEAFKSGKDIRHGREIVQKLRDRTSIRFYGASGLVHFDEYGERNLDYSVYDLQQTETLIQFVSVLDFDSHTKMIKPTLRFSNIVWQNGKPPTDNPSCGFDNELCEWLDNEISLLVLLVALPLIGVAAVSLITLLTLQKLRLQSRLDESNWWLINYSDITILREPKGTQILSVNTTPSKCGSGGSQSMLSSNSCNTREAIYATVGLFQGNEVGIKYLKNQIIPDIKKPSIIAEFNMLKEMKHENLVQFFGVCIDPPNVCIVMQYCKKGSLKDVLGNHEIDLDWMFKLSFAYDIVNGMEYIHKSSLKSHGNLRPSTCLVDSRLQIKLSGFGLWEFKCGTKHRLIPLDSPKYEEMYWTAPEFLREIFYPFNGTQKGDVYSFAIIIRELIYSTEVGPYHDVHLEPKEIIKQLRTPMSEEPLRPTLSADICDERLIPLIKACWSENPDHRPPFGSIRRQLRDACPESHANILDNMVNKLEKYANHLEEVVEERTSQLTVEKSRADKLLSSMLPRYIADQLMAGKSVEPRSYEMVTIFFSDIVGFTTMCSVSSALEVVTLLNDLYSLFDDIIKLYDVYKVETIGDAYMVASGLPISNGTLHAEEISTMALHFLSSIKRFKIRHLPNERLALRIGINSGPVVAGVVGSTMPRYCLFGDTVNTASRMESNSLPLKIHISQSTADILLTIGTFELEERGDIEIKGKGTQKTFWLLSKPGFTFPPTGQDSNPSPKSGTDSCHIKQEKTKAANDGDFKERRTIQRTSAMTKMTDMYTLTVPDV

Target ORF information:

RefSeq Version	XM_009306792.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306792.2

ORF Insert Sequence:

ATGTCAGGAG GATTAGCCTC AGACACAAGT CGACCCATAA GATGGTGGCT GCTGTTTACA 
GCTTTTACAA ACTCTGTCAT TTGCAACAAA GACGGCCAGT TCAAGCTAAC GGTGGGCCTT 
CAGGCTCCTC GGAACATTTC TTACCCTTTC AGTTTGCTCA GGCTGGGGTC AGCGATGCAA 
ATAGCCATGG ACAAGATCAA CTCAAATCCA GCTCTCAGTG GAAACTTCAC CTTTGACTTC 
GTCTACATGG ACACAGACTG TAACGCCAAA ACCTCACTGA GGGGTTTCAT CGAGCAAGTA 
ATGGAGCAAA ATGTGTCCGC GTTATTTGGT CCCCCCTGTC CTGAGGAGGC AGAAGTCACT 
GGGCTCATTG CATCCACTTG GAACATTCCC ATGTTCGGAT TTGTTGGTCA GACACCAAAA 
ATGGATAACA TTCAGGTGTA CGACACCTAT ATAAAAATTG TACCTCCCCT TAAAAGGGTG 
GGAGAGATCC TTCTTAAAGC ATTGGAATTT TTCGGATGGA AGCATGTTGG CATGATCGGA 
GGCGGAGCGG ATGCAAATAC GTGGGACAGT GTGGAGGCCC TTTGGAAGTC TGTGGATCAG 
CAACTCCGAA GCAAAGTAAC AGTGACGATG GGCATTAAGT TTGACACCAG TGACCTGGAA 
CAGGCAAAGA GGAACTTGCA AATCATCTCT AAGGTTGCTA GAGTGGTGGT TGTGCTTGCT 
AATGCTGAGG ACTCCACAGC TATGATGATA GAAGCTCAGA AACTGGGCCT GATGAGTGGA 
GAGTATGTTT TCCTTCTGGT TCAGCAGTTT GAGGTCAGTG GCAAAGTGGA CAACCTGTGG 
AAGACTTCTG TGAGCGAAAC AAATAGAACA ATGCTGAAGG CTTTTGACAT GGTGTTTGTA 
CTAGCTGAGA AGTCCTATGA TGGATATGAC TACTATGACT TCTTCGATCA GGCATATGAT 
AAACTGAAAG GAGATCCTTT TTACAGCAAC CTGTCATCAG TAAAAGAAGT AAGCTCATAT 
GCTGCCTATT TACATGATGC TGTCTTACTT TATGCCATGG GCCTCAAAGA AGCATTTAAA 
AGTGGCAAAG ACATTCGTCA TGGACGAGAA ATTGTCCAAA AACTCAGGGA CAGGACAAGC 
ATTAGATTTT ATGGAGCTTC GGGTTTGGTT CACTTTGATG AATATGGAGA GAGGAACCTA 
GATTATTCAG TATATGACCT GCAACAAACA GAAACTTTAA TACAATTTGT CTCTGTTTTG 
GATTTTGACA GCCATACGAA AATGATCAAA CCCACACTAA GGTTTTCGAA TATAGTTTGG 
CAAAACGGCA AACCCCCTAC AGATAATCCA AGCTGTGGTT TTGATAATGA ACTCTGTGAA 
TGGCTAGACA ATGAGATTTC TCTTCTGGTT CTGCTGGTGG CTTTGCCACT GATTGGAGTG 
GCCGCAGTTT CTTTGATTAC TTTGTTGACT CTGCAGAAGC TGCGCTTACA AAGCCGCCTG 
GATGAATCCA ACTGGTGGCT CATCAACTAC AGTGATATAA CTATCCTCAG AGAGCCCAAA 
GGAACACAAA TCCTGTCTGT AAACACAACA CCAAGCAAAT GTGGCAGTGG TGGCTCTCAG 
TCCATGCTCT CCTCCAACAG CTGTAACACT AGAGAAGCCA TCTACGCTAC CGTCGGACTC 
TTTCAGGGAA ACGAGGTGGG CATAAAGTAT TTGAAAAATC AAATCATCCC TGATATCAAG 
AAACCTTCGA TCATCGCGGA GTTCAATATG CTGAAAGAAA TGAAGCATGA GAACCTGGTA 
CAATTTTTCG GTGTATGTAT TGATCCACCA AATGTCTGTA TCGTCATGCA GTATTGCAAG 
AAAGGGAGCT TGAAGGATGT TCTTGGAAAC CATGAAATTG ATCTGGACTG GATGTTTAAA 
CTGTCCTTTG CGTATGACAT TGTTAATGGC ATGGAGTACA TCCATAAGAG CAGTCTGAAG 
TCTCATGGAA ACCTGAGGCC CAGCACGTGT TTGGTGGACA GCAGGCTTCA GATCAAACTC 
TCCGGCTTTG GCCTGTGGGA GTTCAAATGT GGCACCAAAC ACAGACTGAT TCCGTTGGAC 
AGTCCAAAAT ATGAAGAGAT GTACTGGACG GCTCCTGAGT TTTTGAGAGA GATCTTCTAC 
CCTTTCAATG GCACCCAGAA GGGAGATGTC TATAGCTTTG CCATAATCAT CCGAGAGCTC 
ATATACAGTA CAGAGGTGGG CCCGTATCAT GACGTTCATC TGGAACCAAA AGAAATCATT 
AAGCAGTTAA GGACCCCTAT GAGTGAGGAG CCTCTTAGAC CCACCCTGTC TGCTGACATC 
TGTGACGAAC GTCTCATCCC GTTAATTAAA GCCTGCTGGA GTGAAAACCC AGACCACAGA 
CCCCCGTTCG GCAGTATCAG AAGACAACTG AGAGACGCTT GTCCTGAAAG CCATGCCAAC 
ATCCTGGACA ACATGGTGAA CAAGCTGGAG AAATATGCCA ATCATCTGGA AGAAGTGGTG 
GAGGAGAGAA CCAGTCAGCT GACCGTGGAG AAGAGCAGAG CTGATAAGCT TCTCTCTAGC 
ATGCTGCCCA GGTATATTGC TGATCAGCTA ATGGCTGGAA AATCAGTGGA GCCCAGAAGT 
TATGAAATGG TAACCATCTT CTTCTCTGAC ATTGTGGGTT TCACCACTAT GTGCTCTGTT 
AGCTCTGCTC TGGAGGTGGT CACTCTTCTC AATGACCTCT ACAGCCTTTT TGATGATATC 
ATCAAGCTAT ATGATGTCTA CAAGGTGGAG ACTATTGGAG ATGCCTATAT GGTGGCCAGT 
GGACTTCCTA TCAGTAATGG TACTCTTCAT GCTGAGGAGA TCTCTACTAT GGCATTGCAC 
TTCCTCTCTT CCATCAAAAG GTTCAAAATA AGGCACCTGC CCAATGAGAG ACTAGCCCTA 
CGGATTGGGA TTAATTCTGG CCCAGTAGTT GCTGGAGTAG TGGGTAGTAC AATGCCTCGC 
TACTGTTTAT TTGGAGATAC AGTCAACACA GCTTCTAGGA TGGAGAGCAA TAGTCTTCCA 
CTGAAAATCC ATATCTCTCA GAGCACTGCT GATATTCTCT TGACAATTGG CACATTTGAA 
CTGGAGGAAA GGGGCGATAT TGAAATTAAG GGAAAGGGAA CACAGAAAAC GTTCTGGTTG 
TTGAGCAAAC CTGGATTCAC GTTTCCACCC ACTGGCCAGG ACAGTAATCC AAGTCCTAAA 
TCAGGAACAG ATTCTTGTCA CATCAAACAG GAGAAAACCA AAGCAGCAAA TGATGGAGAT 
TTTAAAGAGA GAAGAACAAT ACAGCGGACA TCGGCAATGA CTAAAATGAC AGACATGTAC 
ACCCTCACTG TACCTGATGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gucy2g ( XM_009306792.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305067.2 Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54524
Clone ID Related Accession (Same CDS sequence)	XM_009306792.2 , XM_009306792.3
Accession Version	XM_009306792.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanylate cyclase 2G-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306792.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAG GATTAGCCTC AGACACAAGT CGACCCATAA GATGGTGGCT GCTGTTTACA 
GCTTTTACAA ACTCTGTCAT TTGCAACAAA GACGGCCAGT TCAAGCTAAC GGTGGGCCTT 
CAGGCTCCTC GGAACATTTC TTACCCTTTC AGTTTGCTCA GGCTGGGGTC AGCGATGCAA 
ATAGCCATGG ACAAGATCAA CTCAAATCCA GCTCTCAGTG GAAACTTCAC CTTTGACTTC 
GTCTACATGG ACACAGACTG TAACGCCAAA ACCTCACTGA GGGGTTTCAT CGAGCAAGTA 
ATGGAGCAAA ATGTGTCCGC GTTATTTGGT CCCCCCTGTC CTGAGGAGGC AGAAGTCACT 
GGGCTCATTG CATCCACTTG GAACATTCCC ATGTTCGGAT TTGTTGGTCA GACACCAAAA 
ATGGATAACA TTCAGGTGTA CGACACCTAT ATAAAAATTG TACCTCCCCT TAAAAGGGTG 
GGAGAGATCC TTCTTAAAGC ATTGGAATTT TTCGGATGGA AGCATGTTGG CATGATCGGA 
GGCGGAGCGG ATGCAAATAC GTGGGACAGT GTGGAGGCCC TTTGGAAGTC TGTGGATCAG 
CAACTCCGAA GCAAAGTAAC AGTGACGATG GGCATTAAGT TTGACACCAG TGACCTGGAA 
CAGGCAAAGA GGAACTTGCA AATCATCTCT AAGGTTGCTA GAGTGGTGGT TGTGCTTGCT 
AATGCTGAGG ACTCCACAGC TATGATGATA GAAGCTCAGA AACTGGGCCT GATGAGTGGA 
GAGTATGTTT TCCTTCTGGT TCAGCAGTTT GAGGTCAGTG GCAAAGTGGA CAACCTGTGG 
AAGACTTCTG TGAGCGAAAC AAATAGAACA ATGCTGAAGG CTTTTGACAT GGTGTTTGTA 
CTAGCTGAGA AGTCCTATGA TGGATATGAC TACTATGACT TCTTCGATCA GGCATATGAT 
AAACTGAAAG GAGATCCTTT TTACAGCAAC CTGTCATCAG TAAAAGAAGT AAGCTCATAT 
GCTGCCTATT TACATGATGC TGTCTTACTT TATGCCATGG GCCTCAAAGA AGCATTTAAA 
AGTGGCAAAG ACATTCGTCA TGGACGAGAA ATTGTCCAAA AACTCAGGGA CAGGACAAGC 
ATTAGATTTT ATGGAGCTTC GGGTTTGGTT CACTTTGATG AATATGGAGA GAGGAACCTA 
GATTATTCAG TATATGACCT GCAACAAACA GAAACTTTAA TACAATTTGT CTCTGTTTTG 
GATTTTGACA GCCATACGAA AATGATCAAA CCCACACTAA GGTTTTCGAA TATAGTTTGG 
CAAAACGGCA AACCCCCTAC AGATAATCCA AGCTGTGGTT TTGATAATGA ACTCTGTGAA 
TGGCTAGACA ATGAGATTTC TCTTCTGGTT CTGCTGGTGG CTTTGCCACT GATTGGAGTG 
GCCGCAGTTT CTTTGATTAC TTTGTTGACT CTGCAGAAGC TGCGCTTACA AAGCCGCCTG 
GATGAATCCA ACTGGTGGCT CATCAACTAC AGTGATATAA CTATCCTCAG AGAGCCCAAA 
GGAACACAAA TCCTGTCTGT AAACACAACA CCAAGCAAAT GTGGCAGTGG TGGCTCTCAG 
TCCATGCTCT CCTCCAACAG CTGTAACACT AGAGAAGCCA TCTACGCTAC CGTCGGACTC 
TTTCAGGGAA ACGAGGTGGG CATAAAGTAT TTGAAAAATC AAATCATCCC TGATATCAAG 
AAACCTTCGA TCATCGCGGA GTTCAATATG CTGAAAGAAA TGAAGCATGA GAACCTGGTA 
CAATTTTTCG GTGTATGTAT TGATCCACCA AATGTCTGTA TCGTCATGCA GTATTGCAAG 
AAAGGGAGCT TGAAGGATGT TCTTGGAAAC CATGAAATTG ATCTGGACTG GATGTTTAAA 
CTGTCCTTTG CGTATGACAT TGTTAATGGC ATGGAGTACA TCCATAAGAG CAGTCTGAAG 
TCTCATGGAA ACCTGAGGCC CAGCACGTGT TTGGTGGACA GCAGGCTTCA GATCAAACTC 
TCCGGCTTTG GCCTGTGGGA GTTCAAATGT GGCACCAAAC ACAGACTGAT TCCGTTGGAC 
AGTCCAAAAT ATGAAGAGAT GTACTGGACG GCTCCTGAGT TTTTGAGAGA GATCTTCTAC 
CCTTTCAATG GCACCCAGAA GGGAGATGTC TATAGCTTTG CCATAATCAT CCGAGAGCTC 
ATATACAGTA CAGAGGTGGG CCCGTATCAT GACGTTCATC TGGAACCAAA AGAAATCATT 
AAGCAGTTAA GGACCCCTAT GAGTGAGGAG CCTCTTAGAC CCACCCTGTC TGCTGACATC 
TGTGACGAAC GTCTCATCCC GTTAATTAAA GCCTGCTGGA GTGAAAACCC AGACCACAGA 
CCCCCGTTCG GCAGTATCAG AAGACAACTG AGAGACGCTT GTCCTGAAAG CCATGCCAAC 
ATCCTGGACA ACATGGTGAA CAAGCTGGAG AAATATGCCA ATCATCTGGA AGAAGTGGTG 
GAGGAGAGAA CCAGTCAGCT GACCGTGGAG AAGAGCAGAG CTGATAAGCT TCTCTCTAGC 
ATGCTGCCCA GGTATATTGC TGATCAGCTA ATGGCTGGAA AATCAGTGGA GCCCAGAAGT 
TATGAAATGG TAACCATCTT CTTCTCTGAC ATTGTGGGTT TCACCACTAT GTGCTCTGTT 
AGCTCTGCTC TGGAGGTGGT CACTCTTCTC AATGACCTCT ACAGCCTTTT TGATGATATC 
ATCAAGCTAT ATGATGTCTA CAAGGTGGAG ACTATTGGAG ATGCCTATAT GGTGGCCAGT 
GGACTTCCTA TCAGTAATGG TACTCTTCAT GCTGAGGAGA TCTCTACTAT GGCATTGCAC 
TTCCTCTCTT CCATCAAAAG GTTCAAAATA AGGCACCTGC CCAATGAGAG ACTAGCCCTA 
CGGATTGGGA TTAATTCTGG CCCAGTAGTT GCTGGAGTAG TGGGTAGTAC AATGCCTCGC 
TACTGTTTAT TTGGAGATAC AGTCAACACA GCTTCTAGGA TGGAGAGCAA TAGTCTTCCA 
CTGAAAATCC ATATCTCTCA GAGCACTGCT GATATTCTCT TGACAATTGG CACATTTGAA 
CTGGAGGAAA GGGGCGATAT TGAAATTAAG GGAAAGGGAA CACAGAAAAC GTTCTGGTTG 
TTGAGCAAAC CTGGATTCAC GTTTCCACCC ACTGGCCAGG ACAGTAATCC AAGTCCTAAA 
TCAGGAACAG ATTCTTGTCA CATCAAACAG GAGAAAACCA AAGCAGCAAA TGATGGAGAT 
TTTAAAGAGA GAAGAACAAT ACAGCGGACA TCGGCAATGA CTAAAATGAC AGACATGTAC 
ACCCTCACTG TACCTGATGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305067.2
CDS	222..3605
Translation	MSGGLASDTSRPIRWWLLFTAFTNSVICNKDGQFKLTVGLQAPRNISYPFSLLRLGSAMQIAMDKINSNPALSGNFTFDFVYMDTDCNAKTSLRGFIEQVMEQNVSALFGPPCPEEAEVTGLIASTWNIPMFGFVGQTPKMDNIQVYDTYIKIVPPLKRVGEILLKALEFFGWKHVGMIGGGADANTWDSVEALWKSVDQQLRSKVTVTMGIKFDTSDLEQAKRNLQIISKVARVVVVLANAEDSTAMMIEAQKLGLMSGEYVFLLVQQFEVSGKVDNLWKTSVSETNRTMLKAFDMVFVLAEKSYDGYDYYDFFDQAYDKLKGDPFYSNLSSVKEVSSYAAYLHDAVLLYAMGLKEAFKSGKDIRHGREIVQKLRDRTSIRFYGASGLVHFDEYGERNLDYSVYDLQQTETLIQFVSVLDFDSHTKMIKPTLRFSNIVWQNGKPPTDNPSCGFDNELCEWLDNEISLLVLLVALPLIGVAAVSLITLLTLQKLRLQSRLDESNWWLINYSDITILREPKGTQILSVNTTPSKCGSGGSQSMLSSNSCNTREAIYATVGLFQGNEVGIKYLKNQIIPDIKKPSIIAEFNMLKEMKHENLVQFFGVCIDPPNVCIVMQYCKKGSLKDVLGNHEIDLDWMFKLSFAYDIVNGMEYIHKSSLKSHGNLRPSTCLVDSRLQIKLSGFGLWEFKCGTKHRLIPLDSPKYEEMYWTAPEFLREIFYPFNGTQKGDVYSFAIIIRELIYSTEVGPYHDVHLEPKEIIKQLRTPMSEEPLRPTLSADICDERLIPLIKACWSENPDHRPPFGSIRRQLRDACPESHANILDNMVNKLEKYANHLEEVVEERTSQLTVEKSRADKLLSSMLPRYIADQLMAGKSVEPRSYEMVTIFFSDIVGFTTMCSVSSALEVVTLLNDLYSLFDDIIKLYDVYKVETIGDAYMVASGLPISNGTLHAEEISTMALHFLSSIKRFKIRHLPNERLALRIGINSGPVVAGVVGSTMPRYCLFGDTVNTASRMESNSLPLKIHISQSTADILLTIGTFELEERGDIEIKGKGTQKTFWLLSKPGFTFPPTGQDSNPSPKSGTDSCHIKQEKTKAANDGDFKERRTIQRTSAMTKMTDMYTLTVPDV

Target ORF information:

RefSeq Version	XM_009306792.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306792.3

ORF Insert Sequence:

ATGTCAGGAG GATTAGCCTC AGACACAAGT CGACCCATAA GATGGTGGCT GCTGTTTACA 
GCTTTTACAA ACTCTGTCAT TTGCAACAAA GACGGCCAGT TCAAGCTAAC GGTGGGCCTT 
CAGGCTCCTC GGAACATTTC TTACCCTTTC AGTTTGCTCA GGCTGGGGTC AGCGATGCAA 
ATAGCCATGG ACAAGATCAA CTCAAATCCA GCTCTCAGTG GAAACTTCAC CTTTGACTTC 
GTCTACATGG ACACAGACTG TAACGCCAAA ACCTCACTGA GGGGTTTCAT CGAGCAAGTA 
ATGGAGCAAA ATGTGTCCGC GTTATTTGGT CCCCCCTGTC CTGAGGAGGC AGAAGTCACT 
GGGCTCATTG CATCCACTTG GAACATTCCC ATGTTCGGAT TTGTTGGTCA GACACCAAAA 
ATGGATAACA TTCAGGTGTA CGACACCTAT ATAAAAATTG TACCTCCCCT TAAAAGGGTG 
GGAGAGATCC TTCTTAAAGC ATTGGAATTT TTCGGATGGA AGCATGTTGG CATGATCGGA 
GGCGGAGCGG ATGCAAATAC GTGGGACAGT GTGGAGGCCC TTTGGAAGTC TGTGGATCAG 
CAACTCCGAA GCAAAGTAAC AGTGACGATG GGCATTAAGT TTGACACCAG TGACCTGGAA 
CAGGCAAAGA GGAACTTGCA AATCATCTCT AAGGTTGCTA GAGTGGTGGT TGTGCTTGCT 
AATGCTGAGG ACTCCACAGC TATGATGATA GAAGCTCAGA AACTGGGCCT GATGAGTGGA 
GAGTATGTTT TCCTTCTGGT TCAGCAGTTT GAGGTCAGTG GCAAAGTGGA CAACCTGTGG 
AAGACTTCTG TGAGCGAAAC AAATAGAACA ATGCTGAAGG CTTTTGACAT GGTGTTTGTA 
CTAGCTGAGA AGTCCTATGA TGGATATGAC TACTATGACT TCTTCGATCA GGCATATGAT 
AAACTGAAAG GAGATCCTTT TTACAGCAAC CTGTCATCAG TAAAAGAAGT AAGCTCATAT 
GCTGCCTATT TACATGATGC TGTCTTACTT TATGCCATGG GCCTCAAAGA AGCATTTAAA 
AGTGGCAAAG ACATTCGTCA TGGACGAGAA ATTGTCCAAA AACTCAGGGA CAGGACAAGC 
ATTAGATTTT ATGGAGCTTC GGGTTTGGTT CACTTTGATG AATATGGAGA GAGGAACCTA 
GATTATTCAG TATATGACCT GCAACAAACA GAAACTTTAA TACAATTTGT CTCTGTTTTG 
GATTTTGACA GCCATACGAA AATGATCAAA CCCACACTAA GGTTTTCGAA TATAGTTTGG 
CAAAACGGCA AACCCCCTAC AGATAATCCA AGCTGTGGTT TTGATAATGA ACTCTGTGAA 
TGGCTAGACA ATGAGATTTC TCTTCTGGTT CTGCTGGTGG CTTTGCCACT GATTGGAGTG 
GCCGCAGTTT CTTTGATTAC TTTGTTGACT CTGCAGAAGC TGCGCTTACA AAGCCGCCTG 
GATGAATCCA ACTGGTGGCT CATCAACTAC AGTGATATAA CTATCCTCAG AGAGCCCAAA 
GGAACACAAA TCCTGTCTGT AAACACAACA CCAAGCAAAT GTGGCAGTGG TGGCTCTCAG 
TCCATGCTCT CCTCCAACAG CTGTAACACT AGAGAAGCCA TCTACGCTAC CGTCGGACTC 
TTTCAGGGAA ACGAGGTGGG CATAAAGTAT TTGAAAAATC AAATCATCCC TGATATCAAG 
AAACCTTCGA TCATCGCGGA GTTCAATATG CTGAAAGAAA TGAAGCATGA GAACCTGGTA 
CAATTTTTCG GTGTATGTAT TGATCCACCA AATGTCTGTA TCGTCATGCA GTATTGCAAG 
AAAGGGAGCT TGAAGGATGT TCTTGGAAAC CATGAAATTG ATCTGGACTG GATGTTTAAA 
CTGTCCTTTG CGTATGACAT TGTTAATGGC ATGGAGTACA TCCATAAGAG CAGTCTGAAG 
TCTCATGGAA ACCTGAGGCC CAGCACGTGT TTGGTGGACA GCAGGCTTCA GATCAAACTC 
TCCGGCTTTG GCCTGTGGGA GTTCAAATGT GGCACCAAAC ACAGACTGAT TCCGTTGGAC 
AGTCCAAAAT ATGAAGAGAT GTACTGGACG GCTCCTGAGT TTTTGAGAGA GATCTTCTAC 
CCTTTCAATG GCACCCAGAA GGGAGATGTC TATAGCTTTG CCATAATCAT CCGAGAGCTC 
ATATACAGTA CAGAGGTGGG CCCGTATCAT GACGTTCATC TGGAACCAAA AGAAATCATT 
AAGCAGTTAA GGACCCCTAT GAGTGAGGAG CCTCTTAGAC CCACCCTGTC TGCTGACATC 
TGTGACGAAC GTCTCATCCC GTTAATTAAA GCCTGCTGGA GTGAAAACCC AGACCACAGA 
CCCCCGTTCG GCAGTATCAG AAGACAACTG AGAGACGCTT GTCCTGAAAG CCATGCCAAC 
ATCCTGGACA ACATGGTGAA CAAGCTGGAG AAATATGCCA ATCATCTGGA AGAAGTGGTG 
GAGGAGAGAA CCAGTCAGCT GACCGTGGAG AAGAGCAGAG CTGATAAGCT TCTCTCTAGC 
ATGCTGCCCA GGTATATTGC TGATCAGCTA ATGGCTGGAA AATCAGTGGA GCCCAGAAGT 
TATGAAATGG TAACCATCTT CTTCTCTGAC ATTGTGGGTT TCACCACTAT GTGCTCTGTT 
AGCTCTGCTC TGGAGGTGGT CACTCTTCTC AATGACCTCT ACAGCCTTTT TGATGATATC 
ATCAAGCTAT ATGATGTCTA CAAGGTGGAG ACTATTGGAG ATGCCTATAT GGTGGCCAGT 
GGACTTCCTA TCAGTAATGG TACTCTTCAT GCTGAGGAGA TCTCTACTAT GGCATTGCAC 
TTCCTCTCTT CCATCAAAAG GTTCAAAATA AGGCACCTGC CCAATGAGAG ACTAGCCCTA 
CGGATTGGGA TTAATTCTGG CCCAGTAGTT GCTGGAGTAG TGGGTAGTAC AATGCCTCGC 
TACTGTTTAT TTGGAGATAC AGTCAACACA GCTTCTAGGA TGGAGAGCAA TAGTCTTCCA 
CTGAAAATCC ATATCTCTCA GAGCACTGCT GATATTCTCT TGACAATTGG CACATTTGAA 
CTGGAGGAAA GGGGCGATAT TGAAATTAAG GGAAAGGGAA CACAGAAAAC GTTCTGGTTG 
TTGAGCAAAC CTGGATTCAC GTTTCCACCC ACTGGCCAGG ACAGTAATCC AAGTCCTAAA 
TCAGGAACAG ATTCTTGTCA CATCAAACAG GAGAAAACCA AAGCAGCAAA TGATGGAGAT 
TTTAAAGAGA GAAGAACAAT ACAGCGGACA TCGGCAATGA CTAAAATGAC AGACATGTAC 
ACCCTCACTG TACCTGATGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gucy2g ( XM_002663981.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002664027.2 Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18041
Clone ID Related Accession (Same CDS sequence)	XM_002663981.5 , XM_002663981.6
Accession Version	XM_002663981.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	guanylate cyclase 2G-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002663981.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAG GATTAGCCTC AGACACAAGT CGACCCATAA GATGGTGGCT GCTGTTTACA 
GCTTTTACAA ACTCTGTCAT TTGCAACAAA GACGGCCAGT TCAAGCTAAC GGTGGGCCTT 
CAGGCTCCTC GGAACATTTC TTACCCTTTC AGTTTGCTCA GGCTGGGGTC AGCGATGCAA 
ATAGCCATGG ACAAGATCAA CTCAAATCCA GCTCTCAGTG GAAACTTCAC CTTTGACTTC 
GTCTACATGG ACACAGACTG TAACGCCAAA ACCTCACTGA GGGGTTTCAT CGAGCAAGTA 
ATGGAGCAAA ATGTGTCCGC GTTATTTGGT CCCCCCTGTC CTGAGGAGGC AGAAGTCACT 
GGGCTCATTG CATCCACTTG GAACATTCCC ATGTTCGGAT TTGTTGGTCA GACACCAAAA 
ATGGATAACA TTCAGGTGTA CGACACCTAT ATAAAAATTG TACCTCCCCT TAAAAGGGTG 
GGAGAGATCC TTCTTAAAGC ATTGGAATTT TTCGGATGGA AGCATGTTGG CATGATCGGA 
GGCGGAGCGG ATGCAAATAC GTGGGACAGT GTGGAGGCCC TTTGGAAGTC TGTGGATCAG 
CAACTCCGAA GCAAAGTAAC AGTGACGATG GGCATTAAGT TTGACACCAG TGACCTGGAA 
CAGGCAAAGA GGAACTTGCA AATCATCTCT AAGGTTGCTA GAGTGGTGGT TGTGCTTGCT 
AATGCTGAGG ACTCCACAGC TATGATGATA GAAGCTCAGA AACTGGGCCT GATGAGTGGA 
GAGTATGTTT TCCTTCTGGT TCAGCAGTTT GAGGACAACC TGTGGAAGAC TTCTGTGAGC 
GAAACAAATA GAACAATGCT GAAGGCTTTT GACATGGTGT TTGTACTAGC TGAGAAGTCC 
TATGATGGAT ATGACTACTA TGACTTCTTC GATCAGGCAT ATGATAAACT GAAAGGAGAT 
CCTTTTTACA GCAACCTGTC ATCAGTAAAA GAAGTAAGCT CATATGCTGC CTATTTACAT 
GATGCTGTCT TACTTTATGC CATGGGCCTC AAAGAAGCAT TTAAAAGTGG CAAAGACATT 
CGTCATGGAC GAGAAATTGT CCAAAAACTC AGGGACAGGA CAAGCATTAG ATTTTATGGA 
GCTTCGGGTT TGGTTCACTT TGATGAATAT GGAGAGAGGA ACCTAGATTA TTCAGTATAT 
GACCTGCAAC AAACAGAAAC TTTAATACAA TTTGTCTCTG TTTTGGATTT TGACAGCCAT 
ACGAAAATGA TCAAACCCAC ACTAAGGTTT TCGAATATAG TTTGGCAAAA CGGCAAACCC 
CCTACAGATA ATCCAAGCTG TGGTTTTGAT AATGAACTCT GTGAATGGCT AGACAATGAG 
ATTTCTCTTC TGGTTCTGCT GGTGGCTTTG CCACTGATTG GAGTGGCCGC AGTTTCTTTG 
ATTACTTTGT TGACTCTGCA GAAGCTGCGC TTACAAAGCC GCCTGGATGA ATCCAACTGG 
TGGCTCATCA ACTACAGTGA TATAACTATC CTCAGAGAGC CCAAAGGAAC ACAAATCCTG 
TCTGTAAACA CAACACCAAG CAAATGTGGC AGTGGTGGCT CTCAGTCCAT GCTCTCCTCC 
AACAGCTGTA ACACTAGAGA AGCCATCTAC GCTACCGTCG GACTCTTTCA GGGAAACGAG 
GTGGGCATAA AGTATTTGAA AAATCAAATC ATCCCTGATA TCAAGAAACC TTCGATCATC 
GCGGAGTTCA ATATGCTGAA AGAAATGAAG CATGAGAACC TGGTACAATT TTTCGGTGTA 
TGTATTGATC CACCAAATGT CTGTATCGTC ATGCAGTATT GCAAGAAAGG GAGCTTGAAG 
GATGTTCTTG GAAACCATGA AATTGATCTG GACTGGATGT TTAAACTGTC CTTTGCGTAT 
GACATTGTTA ATGGCATGGA GTACATCCAT AAGAGCAGTC TGAAGTCTCA TGGAAACCTG 
AGGCCCAGCA CGTGTTTGGT GGACAGCAGG CTTCAGATCA AACTCTCCGG CTTTGGCCTG 
TGGGAGTTCA AATGTGGCAC CAAACACAGA CTGATTCCGT TGGACAGTCC AAAATATGAA 
GAGATGTACT GGACGGCTCC TGAGTTTTTG AGAGAGATCT TCTACCCTTT CAATGGCACC 
CAGAAGGGAG ATGTCTATAG CTTTGCCATA ATCATCCGAG AGCTCATATA CAGTACAGAG 
GTGGGCCCGT ATCATGACGT TCATCTGGAA CCAAAAGAAA TCATTAAGCA GTTAAGGACC 
CCTATGAGTG AGGAGCCTCT TAGACCCACC CTGTCTGCTG ACATCTGTGA CGAACGTCTC 
ATCCCGTTAA TTAAAGCCTG CTGGAGTGAA AACCCAGACC ACAGACCCCC GTTCGGCAGT 
ATCAGAAGAC AACTGAGAGA CGCTTGTCCT GAAAGCCATG CCAACATCCT GGACAACATG 
GTGAACAAGC TGGAGAAATA TGCCAATCAT CTGGAAGAAG TGGTGGAGGA GAGAACCAGT 
CAGCTGACCG TGGAGAAGAG CAGAGCTGAT AAGCTTCTCT CTAGCATGCT GCCCAGGTAT 
ATTGCTGATC AGCTAATGGC TGGAAAATCA GTGGAGCCCA GAAGTTATGA AATGGTAACC 
ATCTTCTTCT CTGACATTGT GGGTTTCACC ACTATGTGCT CTGTTAGCTC TGCTCTGGAG 
GTGGTCACTC TTCTCAATGA CCTCTACAGC CTTTTTGATG ATATCATCAA GCTATATGAT 
GTCTACAAGG TGGAGACTAT TGGAGATGCC TATATGGTGG CCAGTGGACT TCCTATCAGT 
AATGGTACTC TTCATGCTGA GGAGATCTCT ACTATGGCAT TGCACTTCCT CTCTTCCATC 
AAAAGGTTCA AAATAAGGCA CCTGCCCAAT GAGAGACTAG CCCTACGGAT TGGGATTAAT 
TCTGGCCCAG TAGTTGCTGG AGTAGTGGGT AGTACAATGC CTCGCTACTG TTTATTTGGA 
GATACAGTCA ACACAGCTTC TAGGATGGAG AGCAATAGTC TTCCACTGAA AATCCATATC 
TCTCAGAGCA CTGCTGATAT TCTCTTGACA ATTGGCACAT TTGAACTGGA GGAAAGGGGC 
GATATTGAAA TTAAGGGAAA GGGAACACAG AAAACGTTCT GGTTGTTGAG CAAACCTGGA 
TTCACGTTTC CACCCACTGG CCAGGACAGT AATCCAAGTC CTAAATCAGG AACAGATTCT 
TGTCACATCA AACAGGAGAA AACCAAAGCA GCAAATGATG GAGATTTTAA AGAGAGAAGA 
ACAATACAGC GGACATCGGC AATGACTAAA ATGACAGACA TGTACACCCT CACTGTACCT 
GATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002664027.2
CDS	208..3576
Translation	MSGGLASDTSRPIRWWLLFTAFTNSVICNKDGQFKLTVGLQAPRNISYPFSLLRLGSAMQIAMDKINSNPALSGNFTFDFVYMDTDCNAKTSLRGFIEQVMEQNVSALFGPPCPEEAEVTGLIASTWNIPMFGFVGQTPKMDNIQVYDTYIKIVPPLKRVGEILLKALEFFGWKHVGMIGGGADANTWDSVEALWKSVDQQLRSKVTVTMGIKFDTSDLEQAKRNLQIISKVARVVVVLANAEDSTAMMIEAQKLGLMSGEYVFLLVQQFEDNLWKTSVSETNRTMLKAFDMVFVLAEKSYDGYDYYDFFDQAYDKLKGDPFYSNLSSVKEVSSYAAYLHDAVLLYAMGLKEAFKSGKDIRHGREIVQKLRDRTSIRFYGASGLVHFDEYGERNLDYSVYDLQQTETLIQFVSVLDFDSHTKMIKPTLRFSNIVWQNGKPPTDNPSCGFDNELCEWLDNEISLLVLLVALPLIGVAAVSLITLLTLQKLRLQSRLDESNWWLINYSDITILREPKGTQILSVNTTPSKCGSGGSQSMLSSNSCNTREAIYATVGLFQGNEVGIKYLKNQIIPDIKKPSIIAEFNMLKEMKHENLVQFFGVCIDPPNVCIVMQYCKKGSLKDVLGNHEIDLDWMFKLSFAYDIVNGMEYIHKSSLKSHGNLRPSTCLVDSRLQIKLSGFGLWEFKCGTKHRLIPLDSPKYEEMYWTAPEFLREIFYPFNGTQKGDVYSFAIIIRELIYSTEVGPYHDVHLEPKEIIKQLRTPMSEEPLRPTLSADICDERLIPLIKACWSENPDHRPPFGSIRRQLRDACPESHANILDNMVNKLEKYANHLEEVVEERTSQLTVEKSRADKLLSSMLPRYIADQLMAGKSVEPRSYEMVTIFFSDIVGFTTMCSVSSALEVVTLLNDLYSLFDDIIKLYDVYKVETIGDAYMVASGLPISNGTLHAEEISTMALHFLSSIKRFKIRHLPNERLALRIGINSGPVVAGVVGSTMPRYCLFGDTVNTASRMESNSLPLKIHISQSTADILLTIGTFELEERGDIEIKGKGTQKTFWLLSKPGFTFPPTGQDSNPSPKSGTDSCHIKQEKTKAANDGDFKERRTIQRTSAMTKMTDMYTLTVPDV

Target ORF information:

RefSeq Version	XM_002663981.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663981.5

ORF Insert Sequence:

ATGTCAGGAG GATTAGCCTC AGACACAAGT CGACCCATAA GATGGTGGCT GCTGTTTACA 
GCTTTTACAA ACTCTGTCAT TTGCAACAAA GACGGCCAGT TCAAGCTAAC GGTGGGCCTT 
CAGGCTCCTC GGAACATTTC TTACCCTTTC AGTTTGCTCA GGCTGGGGTC AGCGATGCAA 
ATAGCCATGG ACAAGATCAA CTCAAATCCA GCTCTCAGTG GAAACTTCAC CTTTGACTTC 
GTCTACATGG ACACAGACTG TAACGCCAAA ACCTCACTGA GGGGTTTCAT CGAGCAAGTA 
ATGGAGCAAA ATGTGTCCGC GTTATTTGGT CCCCCCTGTC CTGAGGAGGC AGAAGTCACT 
GGGCTCATTG CATCCACTTG GAACATTCCC ATGTTCGGAT TTGTTGGTCA GACACCAAAA 
ATGGATAACA TTCAGGTGTA CGACACCTAT ATAAAAATTG TACCTCCCCT TAAAAGGGTG 
GGAGAGATCC TTCTTAAAGC ATTGGAATTT TTCGGATGGA AGCATGTTGG CATGATCGGA 
GGCGGAGCGG ATGCAAATAC GTGGGACAGT GTGGAGGCCC TTTGGAAGTC TGTGGATCAG 
CAACTCCGAA GCAAAGTAAC AGTGACGATG GGCATTAAGT TTGACACCAG TGACCTGGAA 
CAGGCAAAGA GGAACTTGCA AATCATCTCT AAGGTTGCTA GAGTGGTGGT TGTGCTTGCT 
AATGCTGAGG ACTCCACAGC TATGATGATA GAAGCTCAGA AACTGGGCCT GATGAGTGGA 
GAGTATGTTT TCCTTCTGGT TCAGCAGTTT GAGGACAACC TGTGGAAGAC TTCTGTGAGC 
GAAACAAATA GAACAATGCT GAAGGCTTTT GACATGGTGT TTGTACTAGC TGAGAAGTCC 
TATGATGGAT ATGACTACTA TGACTTCTTC GATCAGGCAT ATGATAAACT GAAAGGAGAT 
CCTTTTTACA GCAACCTGTC ATCAGTAAAA GAAGTAAGCT CATATGCTGC CTATTTACAT 
GATGCTGTCT TACTTTATGC CATGGGCCTC AAAGAAGCAT TTAAAAGTGG CAAAGACATT 
CGTCATGGAC GAGAAATTGT CCAAAAACTC AGGGACAGGA CAAGCATTAG ATTTTATGGA 
GCTTCGGGTT TGGTTCACTT TGATGAATAT GGAGAGAGGA ACCTAGATTA TTCAGTATAT 
GACCTGCAAC AAACAGAAAC TTTAATACAA TTTGTCTCTG TTTTGGATTT TGACAGCCAT 
ACGAAAATGA TCAAACCCAC ACTAAGGTTT TCGAATATAG TTTGGCAAAA CGGCAAACCC 
CCTACAGATA ATCCAAGCTG TGGTTTTGAT AATGAACTCT GTGAATGGCT AGACAATGAG 
ATTTCTCTTC TGGTTCTGCT GGTGGCTTTG CCACTGATTG GAGTGGCCGC AGTTTCTTTG 
ATTACTTTGT TGACTCTGCA GAAGCTGCGC TTACAAAGCC GCCTGGATGA ATCCAACTGG 
TGGCTCATCA ACTACAGTGA TATAACTATC CTCAGAGAGC CCAAAGGAAC ACAAATCCTG 
TCTGTAAACA CAACACCAAG CAAATGTGGC AGTGGTGGCT CTCAGTCCAT GCTCTCCTCC 
AACAGCTGTA ACACTAGAGA AGCCATCTAC GCTACCGTCG GACTCTTTCA GGGAAACGAG 
GTGGGCATAA AGTATTTGAA AAATCAAATC ATCCCTGATA TCAAGAAACC TTCGATCATC 
GCGGAGTTCA ATATGCTGAA AGAAATGAAG CATGAGAACC TGGTACAATT TTTCGGTGTA 
TGTATTGATC CACCAAATGT CTGTATCGTC ATGCAGTATT GCAAGAAAGG GAGCTTGAAG 
GATGTTCTTG GAAACCATGA AATTGATCTG GACTGGATGT TTAAACTGTC CTTTGCGTAT 
GACATTGTTA ATGGCATGGA GTACATCCAT AAGAGCAGTC TGAAGTCTCA TGGAAACCTG 
AGGCCCAGCA CGTGTTTGGT GGACAGCAGG CTTCAGATCA AACTCTCCGG CTTTGGCCTG 
TGGGAGTTCA AATGTGGCAC CAAACACAGA CTGATTCCGT TGGACAGTCC AAAATATGAA 
GAGATGTACT GGACGGCTCC TGAGTTTTTG AGAGAGATCT TCTACCCTTT CAATGGCACC 
CAGAAGGGAG ATGTCTATAG CTTTGCCATA ATCATCCGAG AGCTCATATA CAGTACAGAG 
GTGGGCCCGT ATCATGACGT TCATCTGGAA CCAAAAGAAA TCATTAAGCA GTTAAGGACC 
CCTATGAGTG AGGAGCCTCT TAGACCCACC CTGTCTGCTG ACATCTGTGA CGAACGTCTC 
ATCCCGTTAA TTAAAGCCTG CTGGAGTGAA AACCCAGACC ACAGACCCCC GTTCGGCAGT 
ATCAGAAGAC AACTGAGAGA CGCTTGTCCT GAAAGCCATG CCAACATCCT GGACAACATG 
GTGAACAAGC TGGAGAAATA TGCCAATCAT CTGGAAGAAG TGGTGGAGGA GAGAACCAGT 
CAGCTGACCG TGGAGAAGAG CAGAGCTGAT AAGCTTCTCT CTAGCATGCT GCCCAGGTAT 
ATTGCTGATC AGCTAATGGC TGGAAAATCA GTGGAGCCCA GAAGTTATGA AATGGTAACC 
ATCTTCTTCT CTGACATTGT GGGTTTCACC ACTATGTGCT CTGTTAGCTC TGCTCTGGAG 
GTGGTCACTC TTCTCAATGA CCTCTACAGC CTTTTTGATG ATATCATCAA GCTATATGAT 
GTCTACAAGG TGGAGACTAT TGGAGATGCC TATATGGTGG CCAGTGGACT TCCTATCAGT 
AATGGTACTC TTCATGCTGA GGAGATCTCT ACTATGGCAT TGCACTTCCT CTCTTCCATC 
AAAAGGTTCA AAATAAGGCA CCTGCCCAAT GAGAGACTAG CCCTACGGAT TGGGATTAAT 
TCTGGCCCAG TAGTTGCTGG AGTAGTGGGT AGTACAATGC CTCGCTACTG TTTATTTGGA 
GATACAGTCA ACACAGCTTC TAGGATGGAG AGCAATAGTC TTCCACTGAA AATCCATATC 
TCTCAGAGCA CTGCTGATAT TCTCTTGACA ATTGGCACAT TTGAACTGGA GGAAAGGGGC 
GATATTGAAA TTAAGGGAAA GGGAACACAG AAAACGTTCT GGTTGTTGAG CAAACCTGGA 
TTCACGTTTC CACCCACTGG CCAGGACAGT AATCCAAGTC CTAAATCAGG AACAGATTCT 
TGTCACATCA AACAGGAGAA AACCAAAGCA GCAAATGATG GAGATTTTAA AGAGAGAAGA 
ACAATACAGC GGACATCGGC AATGACTAAA ATGACAGACA TGTACACCCT CACTGTACCT 
GATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gucy2g ( XM_002663981.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002664027.2 Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18041
Clone ID Related Accession (Same CDS sequence)	XM_002663981.5 , XM_002663981.6
Accession Version	XM_002663981.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanylate cyclase 2G-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002663981.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAG GATTAGCCTC AGACACAAGT CGACCCATAA GATGGTGGCT GCTGTTTACA 
GCTTTTACAA ACTCTGTCAT TTGCAACAAA GACGGCCAGT TCAAGCTAAC GGTGGGCCTT 
CAGGCTCCTC GGAACATTTC TTACCCTTTC AGTTTGCTCA GGCTGGGGTC AGCGATGCAA 
ATAGCCATGG ACAAGATCAA CTCAAATCCA GCTCTCAGTG GAAACTTCAC CTTTGACTTC 
GTCTACATGG ACACAGACTG TAACGCCAAA ACCTCACTGA GGGGTTTCAT CGAGCAAGTA 
ATGGAGCAAA ATGTGTCCGC GTTATTTGGT CCCCCCTGTC CTGAGGAGGC AGAAGTCACT 
GGGCTCATTG CATCCACTTG GAACATTCCC ATGTTCGGAT TTGTTGGTCA GACACCAAAA 
ATGGATAACA TTCAGGTGTA CGACACCTAT ATAAAAATTG TACCTCCCCT TAAAAGGGTG 
GGAGAGATCC TTCTTAAAGC ATTGGAATTT TTCGGATGGA AGCATGTTGG CATGATCGGA 
GGCGGAGCGG ATGCAAATAC GTGGGACAGT GTGGAGGCCC TTTGGAAGTC TGTGGATCAG 
CAACTCCGAA GCAAAGTAAC AGTGACGATG GGCATTAAGT TTGACACCAG TGACCTGGAA 
CAGGCAAAGA GGAACTTGCA AATCATCTCT AAGGTTGCTA GAGTGGTGGT TGTGCTTGCT 
AATGCTGAGG ACTCCACAGC TATGATGATA GAAGCTCAGA AACTGGGCCT GATGAGTGGA 
GAGTATGTTT TCCTTCTGGT TCAGCAGTTT GAGGACAACC TGTGGAAGAC TTCTGTGAGC 
GAAACAAATA GAACAATGCT GAAGGCTTTT GACATGGTGT TTGTACTAGC TGAGAAGTCC 
TATGATGGAT ATGACTACTA TGACTTCTTC GATCAGGCAT ATGATAAACT GAAAGGAGAT 
CCTTTTTACA GCAACCTGTC ATCAGTAAAA GAAGTAAGCT CATATGCTGC CTATTTACAT 
GATGCTGTCT TACTTTATGC CATGGGCCTC AAAGAAGCAT TTAAAAGTGG CAAAGACATT 
CGTCATGGAC GAGAAATTGT CCAAAAACTC AGGGACAGGA CAAGCATTAG ATTTTATGGA 
GCTTCGGGTT TGGTTCACTT TGATGAATAT GGAGAGAGGA ACCTAGATTA TTCAGTATAT 
GACCTGCAAC AAACAGAAAC TTTAATACAA TTTGTCTCTG TTTTGGATTT TGACAGCCAT 
ACGAAAATGA TCAAACCCAC ACTAAGGTTT TCGAATATAG TTTGGCAAAA CGGCAAACCC 
CCTACAGATA ATCCAAGCTG TGGTTTTGAT AATGAACTCT GTGAATGGCT AGACAATGAG 
ATTTCTCTTC TGGTTCTGCT GGTGGCTTTG CCACTGATTG GAGTGGCCGC AGTTTCTTTG 
ATTACTTTGT TGACTCTGCA GAAGCTGCGC TTACAAAGCC GCCTGGATGA ATCCAACTGG 
TGGCTCATCA ACTACAGTGA TATAACTATC CTCAGAGAGC CCAAAGGAAC ACAAATCCTG 
TCTGTAAACA CAACACCAAG CAAATGTGGC AGTGGTGGCT CTCAGTCCAT GCTCTCCTCC 
AACAGCTGTA ACACTAGAGA AGCCATCTAC GCTACCGTCG GACTCTTTCA GGGAAACGAG 
GTGGGCATAA AGTATTTGAA AAATCAAATC ATCCCTGATA TCAAGAAACC TTCGATCATC 
GCGGAGTTCA ATATGCTGAA AGAAATGAAG CATGAGAACC TGGTACAATT TTTCGGTGTA 
TGTATTGATC CACCAAATGT CTGTATCGTC ATGCAGTATT GCAAGAAAGG GAGCTTGAAG 
GATGTTCTTG GAAACCATGA AATTGATCTG GACTGGATGT TTAAACTGTC CTTTGCGTAT 
GACATTGTTA ATGGCATGGA GTACATCCAT AAGAGCAGTC TGAAGTCTCA TGGAAACCTG 
AGGCCCAGCA CGTGTTTGGT GGACAGCAGG CTTCAGATCA AACTCTCCGG CTTTGGCCTG 
TGGGAGTTCA AATGTGGCAC CAAACACAGA CTGATTCCGT TGGACAGTCC AAAATATGAA 
GAGATGTACT GGACGGCTCC TGAGTTTTTG AGAGAGATCT TCTACCCTTT CAATGGCACC 
CAGAAGGGAG ATGTCTATAG CTTTGCCATA ATCATCCGAG AGCTCATATA CAGTACAGAG 
GTGGGCCCGT ATCATGACGT TCATCTGGAA CCAAAAGAAA TCATTAAGCA GTTAAGGACC 
CCTATGAGTG AGGAGCCTCT TAGACCCACC CTGTCTGCTG ACATCTGTGA CGAACGTCTC 
ATCCCGTTAA TTAAAGCCTG CTGGAGTGAA AACCCAGACC ACAGACCCCC GTTCGGCAGT 
ATCAGAAGAC AACTGAGAGA CGCTTGTCCT GAAAGCCATG CCAACATCCT GGACAACATG 
GTGAACAAGC TGGAGAAATA TGCCAATCAT CTGGAAGAAG TGGTGGAGGA GAGAACCAGT 
CAGCTGACCG TGGAGAAGAG CAGAGCTGAT AAGCTTCTCT CTAGCATGCT GCCCAGGTAT 
ATTGCTGATC AGCTAATGGC TGGAAAATCA GTGGAGCCCA GAAGTTATGA AATGGTAACC 
ATCTTCTTCT CTGACATTGT GGGTTTCACC ACTATGTGCT CTGTTAGCTC TGCTCTGGAG 
GTGGTCACTC TTCTCAATGA CCTCTACAGC CTTTTTGATG ATATCATCAA GCTATATGAT 
GTCTACAAGG TGGAGACTAT TGGAGATGCC TATATGGTGG CCAGTGGACT TCCTATCAGT 
AATGGTACTC TTCATGCTGA GGAGATCTCT ACTATGGCAT TGCACTTCCT CTCTTCCATC 
AAAAGGTTCA AAATAAGGCA CCTGCCCAAT GAGAGACTAG CCCTACGGAT TGGGATTAAT 
TCTGGCCCAG TAGTTGCTGG AGTAGTGGGT AGTACAATGC CTCGCTACTG TTTATTTGGA 
GATACAGTCA ACACAGCTTC TAGGATGGAG AGCAATAGTC TTCCACTGAA AATCCATATC 
TCTCAGAGCA CTGCTGATAT TCTCTTGACA ATTGGCACAT TTGAACTGGA GGAAAGGGGC 
GATATTGAAA TTAAGGGAAA GGGAACACAG AAAACGTTCT GGTTGTTGAG CAAACCTGGA 
TTCACGTTTC CACCCACTGG CCAGGACAGT AATCCAAGTC CTAAATCAGG AACAGATTCT 
TGTCACATCA AACAGGAGAA AACCAAAGCA GCAAATGATG GAGATTTTAA AGAGAGAAGA 
ACAATACAGC GGACATCGGC AATGACTAAA ATGACAGACA TGTACACCCT CACTGTACCT 
GATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002664027.2
CDS	222..3590
Translation	MSGGLASDTSRPIRWWLLFTAFTNSVICNKDGQFKLTVGLQAPRNISYPFSLLRLGSAMQIAMDKINSNPALSGNFTFDFVYMDTDCNAKTSLRGFIEQVMEQNVSALFGPPCPEEAEVTGLIASTWNIPMFGFVGQTPKMDNIQVYDTYIKIVPPLKRVGEILLKALEFFGWKHVGMIGGGADANTWDSVEALWKSVDQQLRSKVTVTMGIKFDTSDLEQAKRNLQIISKVARVVVVLANAEDSTAMMIEAQKLGLMSGEYVFLLVQQFEDNLWKTSVSETNRTMLKAFDMVFVLAEKSYDGYDYYDFFDQAYDKLKGDPFYSNLSSVKEVSSYAAYLHDAVLLYAMGLKEAFKSGKDIRHGREIVQKLRDRTSIRFYGASGLVHFDEYGERNLDYSVYDLQQTETLIQFVSVLDFDSHTKMIKPTLRFSNIVWQNGKPPTDNPSCGFDNELCEWLDNEISLLVLLVALPLIGVAAVSLITLLTLQKLRLQSRLDESNWWLINYSDITILREPKGTQILSVNTTPSKCGSGGSQSMLSSNSCNTREAIYATVGLFQGNEVGIKYLKNQIIPDIKKPSIIAEFNMLKEMKHENLVQFFGVCIDPPNVCIVMQYCKKGSLKDVLGNHEIDLDWMFKLSFAYDIVNGMEYIHKSSLKSHGNLRPSTCLVDSRLQIKLSGFGLWEFKCGTKHRLIPLDSPKYEEMYWTAPEFLREIFYPFNGTQKGDVYSFAIIIRELIYSTEVGPYHDVHLEPKEIIKQLRTPMSEEPLRPTLSADICDERLIPLIKACWSENPDHRPPFGSIRRQLRDACPESHANILDNMVNKLEKYANHLEEVVEERTSQLTVEKSRADKLLSSMLPRYIADQLMAGKSVEPRSYEMVTIFFSDIVGFTTMCSVSSALEVVTLLNDLYSLFDDIIKLYDVYKVETIGDAYMVASGLPISNGTLHAEEISTMALHFLSSIKRFKIRHLPNERLALRIGINSGPVVAGVVGSTMPRYCLFGDTVNTASRMESNSLPLKIHISQSTADILLTIGTFELEERGDIEIKGKGTQKTFWLLSKPGFTFPPTGQDSNPSPKSGTDSCHIKQEKTKAANDGDFKERRTIQRTSAMTKMTDMYTLTVPDV

Target ORF information:

RefSeq Version	XM_002663981.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663981.6

ORF Insert Sequence:

ATGTCAGGAG GATTAGCCTC AGACACAAGT CGACCCATAA GATGGTGGCT GCTGTTTACA 
GCTTTTACAA ACTCTGTCAT TTGCAACAAA GACGGCCAGT TCAAGCTAAC GGTGGGCCTT 
CAGGCTCCTC GGAACATTTC TTACCCTTTC AGTTTGCTCA GGCTGGGGTC AGCGATGCAA 
ATAGCCATGG ACAAGATCAA CTCAAATCCA GCTCTCAGTG GAAACTTCAC CTTTGACTTC 
GTCTACATGG ACACAGACTG TAACGCCAAA ACCTCACTGA GGGGTTTCAT CGAGCAAGTA 
ATGGAGCAAA ATGTGTCCGC GTTATTTGGT CCCCCCTGTC CTGAGGAGGC AGAAGTCACT 
GGGCTCATTG CATCCACTTG GAACATTCCC ATGTTCGGAT TTGTTGGTCA GACACCAAAA 
ATGGATAACA TTCAGGTGTA CGACACCTAT ATAAAAATTG TACCTCCCCT TAAAAGGGTG 
GGAGAGATCC TTCTTAAAGC ATTGGAATTT TTCGGATGGA AGCATGTTGG CATGATCGGA 
GGCGGAGCGG ATGCAAATAC GTGGGACAGT GTGGAGGCCC TTTGGAAGTC TGTGGATCAG 
CAACTCCGAA GCAAAGTAAC AGTGACGATG GGCATTAAGT TTGACACCAG TGACCTGGAA 
CAGGCAAAGA GGAACTTGCA AATCATCTCT AAGGTTGCTA GAGTGGTGGT TGTGCTTGCT 
AATGCTGAGG ACTCCACAGC TATGATGATA GAAGCTCAGA AACTGGGCCT GATGAGTGGA 
GAGTATGTTT TCCTTCTGGT TCAGCAGTTT GAGGACAACC TGTGGAAGAC TTCTGTGAGC 
GAAACAAATA GAACAATGCT GAAGGCTTTT GACATGGTGT TTGTACTAGC TGAGAAGTCC 
TATGATGGAT ATGACTACTA TGACTTCTTC GATCAGGCAT ATGATAAACT GAAAGGAGAT 
CCTTTTTACA GCAACCTGTC ATCAGTAAAA GAAGTAAGCT CATATGCTGC CTATTTACAT 
GATGCTGTCT TACTTTATGC CATGGGCCTC AAAGAAGCAT TTAAAAGTGG CAAAGACATT 
CGTCATGGAC GAGAAATTGT CCAAAAACTC AGGGACAGGA CAAGCATTAG ATTTTATGGA 
GCTTCGGGTT TGGTTCACTT TGATGAATAT GGAGAGAGGA ACCTAGATTA TTCAGTATAT 
GACCTGCAAC AAACAGAAAC TTTAATACAA TTTGTCTCTG TTTTGGATTT TGACAGCCAT 
ACGAAAATGA TCAAACCCAC ACTAAGGTTT TCGAATATAG TTTGGCAAAA CGGCAAACCC 
CCTACAGATA ATCCAAGCTG TGGTTTTGAT AATGAACTCT GTGAATGGCT AGACAATGAG 
ATTTCTCTTC TGGTTCTGCT GGTGGCTTTG CCACTGATTG GAGTGGCCGC AGTTTCTTTG 
ATTACTTTGT TGACTCTGCA GAAGCTGCGC TTACAAAGCC GCCTGGATGA ATCCAACTGG 
TGGCTCATCA ACTACAGTGA TATAACTATC CTCAGAGAGC CCAAAGGAAC ACAAATCCTG 
TCTGTAAACA CAACACCAAG CAAATGTGGC AGTGGTGGCT CTCAGTCCAT GCTCTCCTCC 
AACAGCTGTA ACACTAGAGA AGCCATCTAC GCTACCGTCG GACTCTTTCA GGGAAACGAG 
GTGGGCATAA AGTATTTGAA AAATCAAATC ATCCCTGATA TCAAGAAACC TTCGATCATC 
GCGGAGTTCA ATATGCTGAA AGAAATGAAG CATGAGAACC TGGTACAATT TTTCGGTGTA 
TGTATTGATC CACCAAATGT CTGTATCGTC ATGCAGTATT GCAAGAAAGG GAGCTTGAAG 
GATGTTCTTG GAAACCATGA AATTGATCTG GACTGGATGT TTAAACTGTC CTTTGCGTAT 
GACATTGTTA ATGGCATGGA GTACATCCAT AAGAGCAGTC TGAAGTCTCA TGGAAACCTG 
AGGCCCAGCA CGTGTTTGGT GGACAGCAGG CTTCAGATCA AACTCTCCGG CTTTGGCCTG 
TGGGAGTTCA AATGTGGCAC CAAACACAGA CTGATTCCGT TGGACAGTCC AAAATATGAA 
GAGATGTACT GGACGGCTCC TGAGTTTTTG AGAGAGATCT TCTACCCTTT CAATGGCACC 
CAGAAGGGAG ATGTCTATAG CTTTGCCATA ATCATCCGAG AGCTCATATA CAGTACAGAG 
GTGGGCCCGT ATCATGACGT TCATCTGGAA CCAAAAGAAA TCATTAAGCA GTTAAGGACC 
CCTATGAGTG AGGAGCCTCT TAGACCCACC CTGTCTGCTG ACATCTGTGA CGAACGTCTC 
ATCCCGTTAA TTAAAGCCTG CTGGAGTGAA AACCCAGACC ACAGACCCCC GTTCGGCAGT 
ATCAGAAGAC AACTGAGAGA CGCTTGTCCT GAAAGCCATG CCAACATCCT GGACAACATG 
GTGAACAAGC TGGAGAAATA TGCCAATCAT CTGGAAGAAG TGGTGGAGGA GAGAACCAGT 
CAGCTGACCG TGGAGAAGAG CAGAGCTGAT AAGCTTCTCT CTAGCATGCT GCCCAGGTAT 
ATTGCTGATC AGCTAATGGC TGGAAAATCA GTGGAGCCCA GAAGTTATGA AATGGTAACC 
ATCTTCTTCT CTGACATTGT GGGTTTCACC ACTATGTGCT CTGTTAGCTC TGCTCTGGAG 
GTGGTCACTC TTCTCAATGA CCTCTACAGC CTTTTTGATG ATATCATCAA GCTATATGAT 
GTCTACAAGG TGGAGACTAT TGGAGATGCC TATATGGTGG CCAGTGGACT TCCTATCAGT 
AATGGTACTC TTCATGCTGA GGAGATCTCT ACTATGGCAT TGCACTTCCT CTCTTCCATC 
AAAAGGTTCA AAATAAGGCA CCTGCCCAAT GAGAGACTAG CCCTACGGAT TGGGATTAAT 
TCTGGCCCAG TAGTTGCTGG AGTAGTGGGT AGTACAATGC CTCGCTACTG TTTATTTGGA 
GATACAGTCA ACACAGCTTC TAGGATGGAG AGCAATAGTC TTCCACTGAA AATCCATATC 
TCTCAGAGCA CTGCTGATAT TCTCTTGACA ATTGGCACAT TTGAACTGGA GGAAAGGGGC 
GATATTGAAA TTAAGGGAAA GGGAACACAG AAAACGTTCT GGTTGTTGAG CAAACCTGGA 
TTCACGTTTC CACCCACTGG CCAGGACAGT AATCCAAGTC CTAAATCAGG AACAGATTCT 
TGTCACATCA AACAGGAGAA AACCAAAGCA GCAAATGATG GAGATTTTAA AGAGAGAAGA 
ACAATACAGC GGACATCGGC AATGACTAAA ATGACAGACA TGTACACCCT CACTGTACCT 
GATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gucy2g ( XM_009306793.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305068.1 Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X3, mRNA.

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CloneID	ODa49811
Clone ID Related Accession (Same CDS sequence)	XM_009306793.2 , XM_009306793.3
Accession Version	XM_009306793.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	guanylate cyclase 2G-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCTC GCACATCATT AAAACAACCA TTACGATATA TTCTCCAGGT CACTGGGCTC 
ATTGCATCCA CTTGGAACAT TCCCATGTTC GGATTTGTTG GTCAGACACC AAAAATGGAT 
AACATTCAGG TGTACGACAC CTATATAAAA ATTGTACCTC CCCTTAAAAG GGTGGGAGAG 
ATCCTTCTTA AAGCATTGGA ATTTTTCGGA TGGAAGCATG TTGGCATGAT CGGAGGCGGA 
GCGGATGCAA ATACGTGGGA CAGTGTGGAG GCCCTTTGGA AGTCTGTGGA TCAGCAACTC 
CGAAGCAAAG TAACAGTGAC GATGGGCATT AAGTTTGACA CCAGTGACCT GGAACAGGCA 
AAGAGGAACT TGCAAATCAT CTCTAAGGTT GCTAGAGTGG TGGTTGTGCT TGCTAATGCT 
GAGGACTCCA CAGCTATGAT GATAGAAGCT CAGAAACTGG GCCTGATGAG TGGAGAGTAT 
GTTTTCCTTC TGGTTCAGCA GTTTGAGGTC AGTGGCAAAG TGGACAACCT GTGGAAGACT 
TCTGTGAGCG AAACAAATAG AACAATGCTG AAGGCTTTTG ACATGGTGTT TGTACTAGCT 
GAGAAGTCCT ATGATGGATA TGACTACTAT GACTTCTTCG ATCAGGCATA TGATAAACTG 
AAAGGAGATC CTTTTTACAG CAACCTGTCA TCAGTAAAAG AAGTAAGCTC ATATGCTGCC 
TATTTACATG ATGCTGTCTT ACTTTATGCC ATGGGCCTCA AAGAAGCATT TAAAAGTGGC 
AAAGACATTC GTCATGGACG AGAAATTGTC CAAAAACTCA GGGACAGGAC AAGCATTAGA 
TTTTATGGAG CTTCGGGTTT GGTTCACTTT GATGAATATG GAGAGAGGAA CCTAGATTAT 
TCAGTATATG ACCTGCAACA AACAGAAACT TTAATACAAT TTGTCTCTGT TTTGGATTTT 
GACAGCCATA CGAAAATGAT CAAACCCACA CTAAGGTTTT CGAATATAGT TTGGCAAAAC 
GGCAAACCCC CTACAGATAA TCCAAGCTGT GGTTTTGATA ATGAACTCTG TGAATGGCTA 
GACAATGAGA TTTCTCTTCT GGTTCTGCTG GTGGCTTTGC CACTGATTGG AGTGGCCGCA 
GTTTCTTTGA TTACTTTGTT GACTCTGCAG AAGCTGCGCT TACAAAGCCG CCTGGATGAA 
TCCAACTGGT GGCTCATCAA CTACAGTGAT ATAACTATCC TCAGAGAGCC CAAAGGAACA 
CAAATCCTGT CTGTAAACAC AACACCAAGC AAATGTGGCA GTGGTGGCTC TCAGTCCATG 
CTCTCCTCCA ACAGCTGTAA CACTAGAGAA GCCATCTACG CTACCGTCGG ACTCTTTCAG 
GGAAACGAGG TGGGCATAAA GTATTTGAAA AATCAAATCA TCCCTGATAT CAAGAAACCT 
TCGATCATCG CGGAGTTCAA TATGCTGAAA GAAATGAAGC ATGAGAACCT GGTACAATTT 
TTCGGTGTAT GTATTGATCC ACCAAATGTC TGTATCGTCA TGCAGTATTG CAAGAAAGGG 
AGCTTGAAGG ATGTTCTTGG AAACCATGAA ATTGATCTGG ACTGGATGTT TAAACTGTCC 
TTTGCGTATG ACATTGTTAA TGGCATGGAG TACATCCATA AGAGCAGTCT GAAGTCTCAT 
GGAAACCTGA GGCCCAGCAC GTGTTTGGTG GACAGCAGGC TTCAGATCAA ACTCTCCGGC 
TTTGGCCTGT GGGAGTTCAA ATGTGGCACC AAACACAGAC TGATTCCGTT GGACAGTCCA 
AAATATGAAG AGATGTACTG GACGGCTCCT GAGTTTTTGA GAGAGATCTT CTACCCTTTC 
AATGGCACCC AGAAGGGAGA TGTCTATAGC TTTGCCATAA TCATCCGAGA GCTCATATAC 
AGTACAGAGG TGGGCCCGTA TCATGACGTT CATCTGGAAC CAAAAGAAAT CATTAAGCAG 
TTAAGGACCC CTATGAGTGA GGAGCCTCTT AGACCCACCC TGTCTGCTGA CATCTGTGAC 
GAACGTCTCA TCCCGTTAAT TAAAGCCTGC TGGAGTGAAA ACCCAGACCA CAGACCCCCG 
TTCGGCAGTA TCAGAAGACA ACTGAGAGAC GCTTGTCCTG AAAGCCATGC CAACATCCTG 
GACAACATGG TGAACAAGCT GGAGAAATAT GCCAATCATC TGGAAGAAGT GGTGGAGGAG 
AGAACCAGTC AGCTGACCGT GGAGAAGAGC AGAGCTGATA AGCTTCTCTC TAGCATGCTG 
CCCAGGTATA TTGCTGATCA GCTAATGGCT GGAAAATCAG TGGAGCCCAG AAGTTATGAA 
ATGGTAACCA TCTTCTTCTC TGACATTGTG GGTTTCACCA CTATGTGCTC TGTTAGCTCT 
GCTCTGGAGG TGGTCACTCT TCTCAATGAC CTCTACAGCC TTTTTGATGA TATCATCAAG 
CTATATGATG TCTACAAGGT GGAGACTATT GGAGATGCCT ATATGGTGGC CAGTGGACTT 
CCTATCAGTA ATGGTACTCT TCATGCTGAG GAGATCTCTA CTATGGCATT GCACTTCCTC 
TCTTCCATCA AAAGGTTCAA AATAAGGCAC CTGCCCAATG AGAGACTAGC CCTACGGATT 
GGGATTAATT CTGGCCCAGT AGTTGCTGGA GTAGTGGGTA GTACAATGCC TCGCTACTGT 
TTATTTGGAG ATACAGTCAA CACAGCTTCT AGGATGGAGA GCAATAGTCT TCCACTGAAA 
ATCCATATCT CTCAGAGCAC TGCTGATATT CTCTTGACAA TTGGCACATT TGAACTGGAG 
GAAAGGGGCG ATATTGAAAT TAAGGGAAAG GGAACACAGA AAACGTTCTG GTTGTTGAGC 
AAACCTGGAT TCACGTTTCC ACCCACTGGC CAGGACAGTA ATCCAAGTCC TAAATCAGGA 
ACAGATTCTT GTCACATCAA ACAGGAGAAA ACCAAAGCAG CAAATGATGG AGATTTTAAA 
GAGAGAAGAA CAATACAGCG GACATCGGCA ATGACTAAAA TGACAGACAT GTACACCCTC 
ACTGTACCTG ATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305068.1
CDS	86..3163
Translation	MSARTSLKQPLRYILQVTGLIASTWNIPMFGFVGQTPKMDNIQVYDTYIKIVPPLKRVGEILLKALEFFGWKHVGMIGGGADANTWDSVEALWKSVDQQLRSKVTVTMGIKFDTSDLEQAKRNLQIISKVARVVVVLANAEDSTAMMIEAQKLGLMSGEYVFLLVQQFEVSGKVDNLWKTSVSETNRTMLKAFDMVFVLAEKSYDGYDYYDFFDQAYDKLKGDPFYSNLSSVKEVSSYAAYLHDAVLLYAMGLKEAFKSGKDIRHGREIVQKLRDRTSIRFYGASGLVHFDEYGERNLDYSVYDLQQTETLIQFVSVLDFDSHTKMIKPTLRFSNIVWQNGKPPTDNPSCGFDNELCEWLDNEISLLVLLVALPLIGVAAVSLITLLTLQKLRLQSRLDESNWWLINYSDITILREPKGTQILSVNTTPSKCGSGGSQSMLSSNSCNTREAIYATVGLFQGNEVGIKYLKNQIIPDIKKPSIIAEFNMLKEMKHENLVQFFGVCIDPPNVCIVMQYCKKGSLKDVLGNHEIDLDWMFKLSFAYDIVNGMEYIHKSSLKSHGNLRPSTCLVDSRLQIKLSGFGLWEFKCGTKHRLIPLDSPKYEEMYWTAPEFLREIFYPFNGTQKGDVYSFAIIIRELIYSTEVGPYHDVHLEPKEIIKQLRTPMSEEPLRPTLSADICDERLIPLIKACWSENPDHRPPFGSIRRQLRDACPESHANILDNMVNKLEKYANHLEEVVEERTSQLTVEKSRADKLLSSMLPRYIADQLMAGKSVEPRSYEMVTIFFSDIVGFTTMCSVSSALEVVTLLNDLYSLFDDIIKLYDVYKVETIGDAYMVASGLPISNGTLHAEEISTMALHFLSSIKRFKIRHLPNERLALRIGINSGPVVAGVVGSTMPRYCLFGDTVNTASRMESNSLPLKIHISQSTADILLTIGTFELEERGDIEIKGKGTQKTFWLLSKPGFTFPPTGQDSNPSPKSGTDSCHIKQEKTKAANDGDFKERRTIQRTSAMTKMTDMYTLTVPDV

Target ORF information:

RefSeq Version	XM_009306793.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306793.2

ORF Insert Sequence:

ATGTCAGCTC GCACATCATT AAAACAACCA TTACGATATA TTCTCCAGGT CACTGGGCTC 
ATTGCATCCA CTTGGAACAT TCCCATGTTC GGATTTGTTG GTCAGACACC AAAAATGGAT 
AACATTCAGG TGTACGACAC CTATATAAAA ATTGTACCTC CCCTTAAAAG GGTGGGAGAG 
ATCCTTCTTA AAGCATTGGA ATTTTTCGGA TGGAAGCATG TTGGCATGAT CGGAGGCGGA 
GCGGATGCAA ATACGTGGGA CAGTGTGGAG GCCCTTTGGA AGTCTGTGGA TCAGCAACTC 
CGAAGCAAAG TAACAGTGAC GATGGGCATT AAGTTTGACA CCAGTGACCT GGAACAGGCA 
AAGAGGAACT TGCAAATCAT CTCTAAGGTT GCTAGAGTGG TGGTTGTGCT TGCTAATGCT 
GAGGACTCCA CAGCTATGAT GATAGAAGCT CAGAAACTGG GCCTGATGAG TGGAGAGTAT 
GTTTTCCTTC TGGTTCAGCA GTTTGAGGTC AGTGGCAAAG TGGACAACCT GTGGAAGACT 
TCTGTGAGCG AAACAAATAG AACAATGCTG AAGGCTTTTG ACATGGTGTT TGTACTAGCT 
GAGAAGTCCT ATGATGGATA TGACTACTAT GACTTCTTCG ATCAGGCATA TGATAAACTG 
AAAGGAGATC CTTTTTACAG CAACCTGTCA TCAGTAAAAG AAGTAAGCTC ATATGCTGCC 
TATTTACATG ATGCTGTCTT ACTTTATGCC ATGGGCCTCA AAGAAGCATT TAAAAGTGGC 
AAAGACATTC GTCATGGACG AGAAATTGTC CAAAAACTCA GGGACAGGAC AAGCATTAGA 
TTTTATGGAG CTTCGGGTTT GGTTCACTTT GATGAATATG GAGAGAGGAA CCTAGATTAT 
TCAGTATATG ACCTGCAACA AACAGAAACT TTAATACAAT TTGTCTCTGT TTTGGATTTT 
GACAGCCATA CGAAAATGAT CAAACCCACA CTAAGGTTTT CGAATATAGT TTGGCAAAAC 
GGCAAACCCC CTACAGATAA TCCAAGCTGT GGTTTTGATA ATGAACTCTG TGAATGGCTA 
GACAATGAGA TTTCTCTTCT GGTTCTGCTG GTGGCTTTGC CACTGATTGG AGTGGCCGCA 
GTTTCTTTGA TTACTTTGTT GACTCTGCAG AAGCTGCGCT TACAAAGCCG CCTGGATGAA 
TCCAACTGGT GGCTCATCAA CTACAGTGAT ATAACTATCC TCAGAGAGCC CAAAGGAACA 
CAAATCCTGT CTGTAAACAC AACACCAAGC AAATGTGGCA GTGGTGGCTC TCAGTCCATG 
CTCTCCTCCA ACAGCTGTAA CACTAGAGAA GCCATCTACG CTACCGTCGG ACTCTTTCAG 
GGAAACGAGG TGGGCATAAA GTATTTGAAA AATCAAATCA TCCCTGATAT CAAGAAACCT 
TCGATCATCG CGGAGTTCAA TATGCTGAAA GAAATGAAGC ATGAGAACCT GGTACAATTT 
TTCGGTGTAT GTATTGATCC ACCAAATGTC TGTATCGTCA TGCAGTATTG CAAGAAAGGG 
AGCTTGAAGG ATGTTCTTGG AAACCATGAA ATTGATCTGG ACTGGATGTT TAAACTGTCC 
TTTGCGTATG ACATTGTTAA TGGCATGGAG TACATCCATA AGAGCAGTCT GAAGTCTCAT 
GGAAACCTGA GGCCCAGCAC GTGTTTGGTG GACAGCAGGC TTCAGATCAA ACTCTCCGGC 
TTTGGCCTGT GGGAGTTCAA ATGTGGCACC AAACACAGAC TGATTCCGTT GGACAGTCCA 
AAATATGAAG AGATGTACTG GACGGCTCCT GAGTTTTTGA GAGAGATCTT CTACCCTTTC 
AATGGCACCC AGAAGGGAGA TGTCTATAGC TTTGCCATAA TCATCCGAGA GCTCATATAC 
AGTACAGAGG TGGGCCCGTA TCATGACGTT CATCTGGAAC CAAAAGAAAT CATTAAGCAG 
TTAAGGACCC CTATGAGTGA GGAGCCTCTT AGACCCACCC TGTCTGCTGA CATCTGTGAC 
GAACGTCTCA TCCCGTTAAT TAAAGCCTGC TGGAGTGAAA ACCCAGACCA CAGACCCCCG 
TTCGGCAGTA TCAGAAGACA ACTGAGAGAC GCTTGTCCTG AAAGCCATGC CAACATCCTG 
GACAACATGG TGAACAAGCT GGAGAAATAT GCCAATCATC TGGAAGAAGT GGTGGAGGAG 
AGAACCAGTC AGCTGACCGT GGAGAAGAGC AGAGCTGATA AGCTTCTCTC TAGCATGCTG 
CCCAGGTATA TTGCTGATCA GCTAATGGCT GGAAAATCAG TGGAGCCCAG AAGTTATGAA 
ATGGTAACCA TCTTCTTCTC TGACATTGTG GGTTTCACCA CTATGTGCTC TGTTAGCTCT 
GCTCTGGAGG TGGTCACTCT TCTCAATGAC CTCTACAGCC TTTTTGATGA TATCATCAAG 
CTATATGATG TCTACAAGGT GGAGACTATT GGAGATGCCT ATATGGTGGC CAGTGGACTT 
CCTATCAGTA ATGGTACTCT TCATGCTGAG GAGATCTCTA CTATGGCATT GCACTTCCTC 
TCTTCCATCA AAAGGTTCAA AATAAGGCAC CTGCCCAATG AGAGACTAGC CCTACGGATT 
GGGATTAATT CTGGCCCAGT AGTTGCTGGA GTAGTGGGTA GTACAATGCC TCGCTACTGT 
TTATTTGGAG ATACAGTCAA CACAGCTTCT AGGATGGAGA GCAATAGTCT TCCACTGAAA 
ATCCATATCT CTCAGAGCAC TGCTGATATT CTCTTGACAA TTGGCACATT TGAACTGGAG 
GAAAGGGGCG ATATTGAAAT TAAGGGAAAG GGAACACAGA AAACGTTCTG GTTGTTGAGC 
AAACCTGGAT TCACGTTTCC ACCCACTGGC CAGGACAGTA ATCCAAGTCC TAAATCAGGA 
ACAGATTCTT GTCACATCAA ACAGGAGAAA ACCAAAGCAG CAAATGATGG AGATTTTAAA 
GAGAGAAGAA CAATACAGCG GACATCGGCA ATGACTAAAA TGACAGACAT GTACACCCTC 
ACTGTACCTG ATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gucy2g ( XM_009306793.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305068.1 Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X3, mRNA.

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CloneID	ODa49811
Clone ID Related Accession (Same CDS sequence)	XM_009306793.2 , XM_009306793.3
Accession Version	XM_009306793.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanylate cyclase 2G-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306793.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCTC GCACATCATT AAAACAACCA TTACGATATA TTCTCCAGGT CACTGGGCTC 
ATTGCATCCA CTTGGAACAT TCCCATGTTC GGATTTGTTG GTCAGACACC AAAAATGGAT 
AACATTCAGG TGTACGACAC CTATATAAAA ATTGTACCTC CCCTTAAAAG GGTGGGAGAG 
ATCCTTCTTA AAGCATTGGA ATTTTTCGGA TGGAAGCATG TTGGCATGAT CGGAGGCGGA 
GCGGATGCAA ATACGTGGGA CAGTGTGGAG GCCCTTTGGA AGTCTGTGGA TCAGCAACTC 
CGAAGCAAAG TAACAGTGAC GATGGGCATT AAGTTTGACA CCAGTGACCT GGAACAGGCA 
AAGAGGAACT TGCAAATCAT CTCTAAGGTT GCTAGAGTGG TGGTTGTGCT TGCTAATGCT 
GAGGACTCCA CAGCTATGAT GATAGAAGCT CAGAAACTGG GCCTGATGAG TGGAGAGTAT 
GTTTTCCTTC TGGTTCAGCA GTTTGAGGTC AGTGGCAAAG TGGACAACCT GTGGAAGACT 
TCTGTGAGCG AAACAAATAG AACAATGCTG AAGGCTTTTG ACATGGTGTT TGTACTAGCT 
GAGAAGTCCT ATGATGGATA TGACTACTAT GACTTCTTCG ATCAGGCATA TGATAAACTG 
AAAGGAGATC CTTTTTACAG CAACCTGTCA TCAGTAAAAG AAGTAAGCTC ATATGCTGCC 
TATTTACATG ATGCTGTCTT ACTTTATGCC ATGGGCCTCA AAGAAGCATT TAAAAGTGGC 
AAAGACATTC GTCATGGACG AGAAATTGTC CAAAAACTCA GGGACAGGAC AAGCATTAGA 
TTTTATGGAG CTTCGGGTTT GGTTCACTTT GATGAATATG GAGAGAGGAA CCTAGATTAT 
TCAGTATATG ACCTGCAACA AACAGAAACT TTAATACAAT TTGTCTCTGT TTTGGATTTT 
GACAGCCATA CGAAAATGAT CAAACCCACA CTAAGGTTTT CGAATATAGT TTGGCAAAAC 
GGCAAACCCC CTACAGATAA TCCAAGCTGT GGTTTTGATA ATGAACTCTG TGAATGGCTA 
GACAATGAGA TTTCTCTTCT GGTTCTGCTG GTGGCTTTGC CACTGATTGG AGTGGCCGCA 
GTTTCTTTGA TTACTTTGTT GACTCTGCAG AAGCTGCGCT TACAAAGCCG CCTGGATGAA 
TCCAACTGGT GGCTCATCAA CTACAGTGAT ATAACTATCC TCAGAGAGCC CAAAGGAACA 
CAAATCCTGT CTGTAAACAC AACACCAAGC AAATGTGGCA GTGGTGGCTC TCAGTCCATG 
CTCTCCTCCA ACAGCTGTAA CACTAGAGAA GCCATCTACG CTACCGTCGG ACTCTTTCAG 
GGAAACGAGG TGGGCATAAA GTATTTGAAA AATCAAATCA TCCCTGATAT CAAGAAACCT 
TCGATCATCG CGGAGTTCAA TATGCTGAAA GAAATGAAGC ATGAGAACCT GGTACAATTT 
TTCGGTGTAT GTATTGATCC ACCAAATGTC TGTATCGTCA TGCAGTATTG CAAGAAAGGG 
AGCTTGAAGG ATGTTCTTGG AAACCATGAA ATTGATCTGG ACTGGATGTT TAAACTGTCC 
TTTGCGTATG ACATTGTTAA TGGCATGGAG TACATCCATA AGAGCAGTCT GAAGTCTCAT 
GGAAACCTGA GGCCCAGCAC GTGTTTGGTG GACAGCAGGC TTCAGATCAA ACTCTCCGGC 
TTTGGCCTGT GGGAGTTCAA ATGTGGCACC AAACACAGAC TGATTCCGTT GGACAGTCCA 
AAATATGAAG AGATGTACTG GACGGCTCCT GAGTTTTTGA GAGAGATCTT CTACCCTTTC 
AATGGCACCC AGAAGGGAGA TGTCTATAGC TTTGCCATAA TCATCCGAGA GCTCATATAC 
AGTACAGAGG TGGGCCCGTA TCATGACGTT CATCTGGAAC CAAAAGAAAT CATTAAGCAG 
TTAAGGACCC CTATGAGTGA GGAGCCTCTT AGACCCACCC TGTCTGCTGA CATCTGTGAC 
GAACGTCTCA TCCCGTTAAT TAAAGCCTGC TGGAGTGAAA ACCCAGACCA CAGACCCCCG 
TTCGGCAGTA TCAGAAGACA ACTGAGAGAC GCTTGTCCTG AAAGCCATGC CAACATCCTG 
GACAACATGG TGAACAAGCT GGAGAAATAT GCCAATCATC TGGAAGAAGT GGTGGAGGAG 
AGAACCAGTC AGCTGACCGT GGAGAAGAGC AGAGCTGATA AGCTTCTCTC TAGCATGCTG 
CCCAGGTATA TTGCTGATCA GCTAATGGCT GGAAAATCAG TGGAGCCCAG AAGTTATGAA 
ATGGTAACCA TCTTCTTCTC TGACATTGTG GGTTTCACCA CTATGTGCTC TGTTAGCTCT 
GCTCTGGAGG TGGTCACTCT TCTCAATGAC CTCTACAGCC TTTTTGATGA TATCATCAAG 
CTATATGATG TCTACAAGGT GGAGACTATT GGAGATGCCT ATATGGTGGC CAGTGGACTT 
CCTATCAGTA ATGGTACTCT TCATGCTGAG GAGATCTCTA CTATGGCATT GCACTTCCTC 
TCTTCCATCA AAAGGTTCAA AATAAGGCAC CTGCCCAATG AGAGACTAGC CCTACGGATT 
GGGATTAATT CTGGCCCAGT AGTTGCTGGA GTAGTGGGTA GTACAATGCC TCGCTACTGT 
TTATTTGGAG ATACAGTCAA CACAGCTTCT AGGATGGAGA GCAATAGTCT TCCACTGAAA 
ATCCATATCT CTCAGAGCAC TGCTGATATT CTCTTGACAA TTGGCACATT TGAACTGGAG 
GAAAGGGGCG ATATTGAAAT TAAGGGAAAG GGAACACAGA AAACGTTCTG GTTGTTGAGC 
AAACCTGGAT TCACGTTTCC ACCCACTGGC CAGGACAGTA ATCCAAGTCC TAAATCAGGA 
ACAGATTCTT GTCACATCAA ACAGGAGAAA ACCAAAGCAG CAAATGATGG AGATTTTAAA 
GAGAGAAGAA CAATACAGCG GACATCGGCA ATGACTAAAA TGACAGACAT GTACACCCTC 
ACTGTACCTG ATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305068.1
CDS	86..3163
Translation	MSARTSLKQPLRYILQVTGLIASTWNIPMFGFVGQTPKMDNIQVYDTYIKIVPPLKRVGEILLKALEFFGWKHVGMIGGGADANTWDSVEALWKSVDQQLRSKVTVTMGIKFDTSDLEQAKRNLQIISKVARVVVVLANAEDSTAMMIEAQKLGLMSGEYVFLLVQQFEVSGKVDNLWKTSVSETNRTMLKAFDMVFVLAEKSYDGYDYYDFFDQAYDKLKGDPFYSNLSSVKEVSSYAAYLHDAVLLYAMGLKEAFKSGKDIRHGREIVQKLRDRTSIRFYGASGLVHFDEYGERNLDYSVYDLQQTETLIQFVSVLDFDSHTKMIKPTLRFSNIVWQNGKPPTDNPSCGFDNELCEWLDNEISLLVLLVALPLIGVAAVSLITLLTLQKLRLQSRLDESNWWLINYSDITILREPKGTQILSVNTTPSKCGSGGSQSMLSSNSCNTREAIYATVGLFQGNEVGIKYLKNQIIPDIKKPSIIAEFNMLKEMKHENLVQFFGVCIDPPNVCIVMQYCKKGSLKDVLGNHEIDLDWMFKLSFAYDIVNGMEYIHKSSLKSHGNLRPSTCLVDSRLQIKLSGFGLWEFKCGTKHRLIPLDSPKYEEMYWTAPEFLREIFYPFNGTQKGDVYSFAIIIRELIYSTEVGPYHDVHLEPKEIIKQLRTPMSEEPLRPTLSADICDERLIPLIKACWSENPDHRPPFGSIRRQLRDACPESHANILDNMVNKLEKYANHLEEVVEERTSQLTVEKSRADKLLSSMLPRYIADQLMAGKSVEPRSYEMVTIFFSDIVGFTTMCSVSSALEVVTLLNDLYSLFDDIIKLYDVYKVETIGDAYMVASGLPISNGTLHAEEISTMALHFLSSIKRFKIRHLPNERLALRIGINSGPVVAGVVGSTMPRYCLFGDTVNTASRMESNSLPLKIHISQSTADILLTIGTFELEERGDIEIKGKGTQKTFWLLSKPGFTFPPTGQDSNPSPKSGTDSCHIKQEKTKAANDGDFKERRTIQRTSAMTKMTDMYTLTVPDV

Target ORF information:

RefSeq Version	XM_009306793.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanylate cyclase 2g (gucy2g), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306793.3

ORF Insert Sequence:

ATGTCAGCTC GCACATCATT AAAACAACCA TTACGATATA TTCTCCAGGT CACTGGGCTC 
ATTGCATCCA CTTGGAACAT TCCCATGTTC GGATTTGTTG GTCAGACACC AAAAATGGAT 
AACATTCAGG TGTACGACAC CTATATAAAA ATTGTACCTC CCCTTAAAAG GGTGGGAGAG 
ATCCTTCTTA AAGCATTGGA ATTTTTCGGA TGGAAGCATG TTGGCATGAT CGGAGGCGGA 
GCGGATGCAA ATACGTGGGA CAGTGTGGAG GCCCTTTGGA AGTCTGTGGA TCAGCAACTC 
CGAAGCAAAG TAACAGTGAC GATGGGCATT AAGTTTGACA CCAGTGACCT GGAACAGGCA 
AAGAGGAACT TGCAAATCAT CTCTAAGGTT GCTAGAGTGG TGGTTGTGCT TGCTAATGCT 
GAGGACTCCA CAGCTATGAT GATAGAAGCT CAGAAACTGG GCCTGATGAG TGGAGAGTAT 
GTTTTCCTTC TGGTTCAGCA GTTTGAGGTC AGTGGCAAAG TGGACAACCT GTGGAAGACT 
TCTGTGAGCG AAACAAATAG AACAATGCTG AAGGCTTTTG ACATGGTGTT TGTACTAGCT 
GAGAAGTCCT ATGATGGATA TGACTACTAT GACTTCTTCG ATCAGGCATA TGATAAACTG 
AAAGGAGATC CTTTTTACAG CAACCTGTCA TCAGTAAAAG AAGTAAGCTC ATATGCTGCC 
TATTTACATG ATGCTGTCTT ACTTTATGCC ATGGGCCTCA AAGAAGCATT TAAAAGTGGC 
AAAGACATTC GTCATGGACG AGAAATTGTC CAAAAACTCA GGGACAGGAC AAGCATTAGA 
TTTTATGGAG CTTCGGGTTT GGTTCACTTT GATGAATATG GAGAGAGGAA CCTAGATTAT 
TCAGTATATG ACCTGCAACA AACAGAAACT TTAATACAAT TTGTCTCTGT TTTGGATTTT 
GACAGCCATA CGAAAATGAT CAAACCCACA CTAAGGTTTT CGAATATAGT TTGGCAAAAC 
GGCAAACCCC CTACAGATAA TCCAAGCTGT GGTTTTGATA ATGAACTCTG TGAATGGCTA 
GACAATGAGA TTTCTCTTCT GGTTCTGCTG GTGGCTTTGC CACTGATTGG AGTGGCCGCA 
GTTTCTTTGA TTACTTTGTT GACTCTGCAG AAGCTGCGCT TACAAAGCCG CCTGGATGAA 
TCCAACTGGT GGCTCATCAA CTACAGTGAT ATAACTATCC TCAGAGAGCC CAAAGGAACA 
CAAATCCTGT CTGTAAACAC AACACCAAGC AAATGTGGCA GTGGTGGCTC TCAGTCCATG 
CTCTCCTCCA ACAGCTGTAA CACTAGAGAA GCCATCTACG CTACCGTCGG ACTCTTTCAG 
GGAAACGAGG TGGGCATAAA GTATTTGAAA AATCAAATCA TCCCTGATAT CAAGAAACCT 
TCGATCATCG CGGAGTTCAA TATGCTGAAA GAAATGAAGC ATGAGAACCT GGTACAATTT 
TTCGGTGTAT GTATTGATCC ACCAAATGTC TGTATCGTCA TGCAGTATTG CAAGAAAGGG 
AGCTTGAAGG ATGTTCTTGG AAACCATGAA ATTGATCTGG ACTGGATGTT TAAACTGTCC 
TTTGCGTATG ACATTGTTAA TGGCATGGAG TACATCCATA AGAGCAGTCT GAAGTCTCAT 
GGAAACCTGA GGCCCAGCAC GTGTTTGGTG GACAGCAGGC TTCAGATCAA ACTCTCCGGC 
TTTGGCCTGT GGGAGTTCAA ATGTGGCACC AAACACAGAC TGATTCCGTT GGACAGTCCA 
AAATATGAAG AGATGTACTG GACGGCTCCT GAGTTTTTGA GAGAGATCTT CTACCCTTTC 
AATGGCACCC AGAAGGGAGA TGTCTATAGC TTTGCCATAA TCATCCGAGA GCTCATATAC 
AGTACAGAGG TGGGCCCGTA TCATGACGTT CATCTGGAAC CAAAAGAAAT CATTAAGCAG 
TTAAGGACCC CTATGAGTGA GGAGCCTCTT AGACCCACCC TGTCTGCTGA CATCTGTGAC 
GAACGTCTCA TCCCGTTAAT TAAAGCCTGC TGGAGTGAAA ACCCAGACCA CAGACCCCCG 
TTCGGCAGTA TCAGAAGACA ACTGAGAGAC GCTTGTCCTG AAAGCCATGC CAACATCCTG 
GACAACATGG TGAACAAGCT GGAGAAATAT GCCAATCATC TGGAAGAAGT GGTGGAGGAG 
AGAACCAGTC AGCTGACCGT GGAGAAGAGC AGAGCTGATA AGCTTCTCTC TAGCATGCTG 
CCCAGGTATA TTGCTGATCA GCTAATGGCT GGAAAATCAG TGGAGCCCAG AAGTTATGAA 
ATGGTAACCA TCTTCTTCTC TGACATTGTG GGTTTCACCA CTATGTGCTC TGTTAGCTCT 
GCTCTGGAGG TGGTCACTCT TCTCAATGAC CTCTACAGCC TTTTTGATGA TATCATCAAG 
CTATATGATG TCTACAAGGT GGAGACTATT GGAGATGCCT ATATGGTGGC CAGTGGACTT 
CCTATCAGTA ATGGTACTCT TCATGCTGAG GAGATCTCTA CTATGGCATT GCACTTCCTC 
TCTTCCATCA AAAGGTTCAA AATAAGGCAC CTGCCCAATG AGAGACTAGC CCTACGGATT 
GGGATTAATT CTGGCCCAGT AGTTGCTGGA GTAGTGGGTA GTACAATGCC TCGCTACTGT 
TTATTTGGAG ATACAGTCAA CACAGCTTCT AGGATGGAGA GCAATAGTCT TCCACTGAAA 
ATCCATATCT CTCAGAGCAC TGCTGATATT CTCTTGACAA TTGGCACATT TGAACTGGAG 
GAAAGGGGCG ATATTGAAAT TAAGGGAAAG GGAACACAGA AAACGTTCTG GTTGTTGAGC 
AAACCTGGAT TCACGTTTCC ACCCACTGGC CAGGACAGTA ATCCAAGTCC TAAATCAGGA 
ACAGATTCTT GTCACATCAA ACAGGAGAAA ACCAAAGCAG CAAATGATGG AGATTTTAAA 
GAGAGAAGAA CAATACAGCG GACATCGGCA ATGACTAAAA TGACAGACAT GTACACCCTC 
ACTGTACCTG ATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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gucy2g cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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