ambra1a cDNA ORF clone, Danio rerio(zebrafish)

The following ambra1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ambra1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa35863 NM_001281992.1
Latest version!
Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa13301 XM_002667669.6
Latest version!
Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa13301 XM_002667669.5 Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa35863
    Clone ID Related Accession (Same CDS sequence) NM_001281992.1
    Accession Version NM_001281992.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3948bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-29
    Organism Danio rerio(zebrafish)
    Product activating molecule in BECN1-regulated autophagy protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from HE602022.1. On Aug 31, 2013 this sequence version replaced XM_005166436.1. ##Evidence-Data-START## Transcript exon combination :: HE602022.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
    GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA
    TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT
    AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG
    GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA
    ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGATGG AGAGGTCAGA
    ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA
    TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT
    CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG
    AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC
    CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT
    CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC
    AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT
    GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG
    CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT
    GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC
    ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT
    GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC
    AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG
    GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC
    AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA
    CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC
    ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC
    TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA
    ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG
    CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC
    GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC
    TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC
    ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT
    GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA
    CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT
    AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA
    AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC
    TTGCGCAGGT TGTCCCCTGC AGCATATTAT GCACAGCGTA TGATCCAGTA TCTGTCTCGA
    CGGGACAGCG TTCGGCAGCA CTCCCACCGT CCCCCTAGCA GACCTCGACC CCTGTCCTCT
    AATCCCAGCA GTCTTTCCCC GAGCCCTGTC CCTAACGCAG AGAGCAGCGA AGTTGACTTT
    GAGGAGTTTG AGGAAAATGG AAGCAGATAT CGGACTCCTC GTAATGCTCG CATGTCTGCC
    CCCTCTCTTG GTCGTTTTGT TGGAACCAGG CGCTTTCTCT TGCCGGAGTT TTTGCCGTAT
    GCTGGAATCT TCCATGAGCG AGGGCAGCCA GGCCTGGCAA CGCACTCATC CGTCAATAGG
    GTCCTCGCAG GTGCTGTAAT TGGAGACGGT CAATCTGCAG TGGCCAGCAA CATCGCTAAC
    ACTACCTATC GTCTGCAGTG GTGGGACTTC ACCAAATTTG ACTTGCCTGA AATCAGTAAT
    GCGTCGGTGA ACGTTCTGGT GCCTAACTGT AAGATCTACA ACGATGCGAG CTGTGATATC
    TCAGCGGATG GGCAGTTGCT GGCCGTATTC ATTCCCAGCA GTCAGAGGGG TTTCCCTGAT
    GAGGGCATTC TGGCTGTTTA CTCTCTCGCT CCGCACAACC TGGGAGAGAT GCTCTACTCC
    AAAAGATTCG GTCCAAATGC CATCTCTGTC AGTTTGTCTC CGATGGGTCG TTACGTGATG
    GTGGGTCTGG CGTCTCGCCG CATCTTGCTG CATCAGATCA GCGATCACAT GGTGGCACAG
    GTCTTCAGAC TGCAGCAGCC TCACGCAGGA GAGACGTCCA TGAGGAGGGT GTTTGATGTG
    GTGTACCCAA TGGCACCAGA CCAGAGGAGA CACGTCAGTA TAAACTCTGC CCGCTGGCTG
    CCAGATCCGG GGCTTGGGCT CGCTTATGGG ACCAACAAAG GAGATCTTGT CATCTGCAGA
    CCTGTCGATG TGCATTCTGA TGGCAGCAGC ACCTCTGAAC ACTCTGAGCG AATGTTCACC
    ATCAATAATG GTGGAGGGGT TGGACCCAGT AGCAGCAGGA GTGGAGACAG GGCAGGGAGC
    AGCAGGACTG ATCGACGGTC ACGCAGAGAC ATAGGCCTGA TGAATGGAGT TGGTCTGCAG
    CCACAACCTC CCGCCGCTAG TGTTACATCA CAGGGGACGC AGACACAGAA CCAGCGACTA
    CAGCATGCAG AGACACAGAC CGACCGCGAT CTTCCAGATG ACCCCCAGCA GCCCAGCACT
    TCTCAGGGGT CACAGGTCAC TGATGCCACA GAATCTCTGG ATTTTGAGAC TCTGCCAGAG
    GATTCTGGAT CTGAGGTGGT ACCCGAGACA CCACCCCACA GCCGGCCTCA GGAAGACGAA
    GGGTCAGATC CCAGCGAACC CAGCACAGAC AGCACAGGTC AGGCTGAATA TGTTTCTCGT
    ATCCGAAGGT TAATGGCTGA GGGAGGCATG ACTGCGGTTG TCCAGCGTGA GCAGAGCACC
    ACAATGGCGT CCATGGGCAG CTTTGGCAAC AATATCATCG TCAGCCATCG CATCCACCGA
    GGCTCCCAGA CAGGAGCGGA TGCACAAAAT CGTACTCGTT TAAGTCCAAT ACCTGGTCCT
    TCTTCTGGTG CTCCGGAGTC TTTAGCTGCA GCTTCGTATT CGCGCGTCCT CACTAATACA
    TTAGGCTTTC GTGGCGACAC AGCTCAAGGC ATAGATCTCA CAGAACAGGA GAGACTGCAC
    ACATCCTTCT TTACACCGGA GTTCTCTCCT CTTTTCTCAT CTGCTGTCGA TGCAACCGGT
    CCTTCCTCCT CAATAGGAGC AGACAGCGTC CTGGAGGGAG AAGACTTTCA CGACTTTGCG
    TCTCTCCCTC CATCTTTACT CTCATCCTCG CCGTCTCTTT CACCAGTGAA TAACAGTAAC
    TACAGCAACA GCGATAGTAG TTATCTTGGA GATGAGTACG GGCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001268921.1
    CDS308..4255
    Translation

    Target ORF information:

    RefSeq Version NM_001281992.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001281992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
    GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA
    TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT
    AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG
    GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA
    ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGATGG AGAGGTCAGA
    ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA
    TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT
    CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG
    AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC
    CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT
    CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC
    AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT
    GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG
    CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT
    GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC
    ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT
    GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC
    AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG
    GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC
    AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA
    CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC
    ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC
    TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA
    ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG
    CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC
    GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC
    TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC
    ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT
    GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA
    CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT
    AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA
    AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC
    TTGCGCAGGT TGTCCCCTGC AGCATATTAT GCACAGCGTA TGATCCAGTA TCTGTCTCGA
    CGGGACAGCG TTCGGCAGCA CTCCCACCGT CCCCCTAGCA GACCTCGACC CCTGTCCTCT
    AATCCCAGCA GTCTTTCCCC GAGCCCTGTC CCTAACGCAG AGAGCAGCGA AGTTGACTTT
    GAGGAGTTTG AGGAAAATGG AAGCAGATAT CGGACTCCTC GTAATGCTCG CATGTCTGCC
    CCCTCTCTTG GTCGTTTTGT TGGAACCAGG CGCTTTCTCT TGCCGGAGTT TTTGCCGTAT
    GCTGGAATCT TCCATGAGCG AGGGCAGCCA GGCCTGGCAA CGCACTCATC CGTCAATAGG
    GTCCTCGCAG GTGCTGTAAT TGGAGACGGT CAATCTGCAG TGGCCAGCAA CATCGCTAAC
    ACTACCTATC GTCTGCAGTG GTGGGACTTC ACCAAATTTG ACTTGCCTGA AATCAGTAAT
    GCGTCGGTGA ACGTTCTGGT GCCTAACTGT AAGATCTACA ACGATGCGAG CTGTGATATC
    TCAGCGGATG GGCAGTTGCT GGCCGTATTC ATTCCCAGCA GTCAGAGGGG TTTCCCTGAT
    GAGGGCATTC TGGCTGTTTA CTCTCTCGCT CCGCACAACC TGGGAGAGAT GCTCTACTCC
    AAAAGATTCG GTCCAAATGC CATCTCTGTC AGTTTGTCTC CGATGGGTCG TTACGTGATG
    GTGGGTCTGG CGTCTCGCCG CATCTTGCTG CATCAGATCA GCGATCACAT GGTGGCACAG
    GTCTTCAGAC TGCAGCAGCC TCACGCAGGA GAGACGTCCA TGAGGAGGGT GTTTGATGTG
    GTGTACCCAA TGGCACCAGA CCAGAGGAGA CACGTCAGTA TAAACTCTGC CCGCTGGCTG
    CCAGATCCGG GGCTTGGGCT CGCTTATGGG ACCAACAAAG GAGATCTTGT CATCTGCAGA
    CCTGTCGATG TGCATTCTGA TGGCAGCAGC ACCTCTGAAC ACTCTGAGCG AATGTTCACC
    ATCAATAATG GTGGAGGGGT TGGACCCAGT AGCAGCAGGA GTGGAGACAG GGCAGGGAGC
    AGCAGGACTG ATCGACGGTC ACGCAGAGAC ATAGGCCTGA TGAATGGAGT TGGTCTGCAG
    CCACAACCTC CCGCCGCTAG TGTTACATCA CAGGGGACGC AGACACAGAA CCAGCGACTA
    CAGCATGCAG AGACACAGAC CGACCGCGAT CTTCCAGATG ACCCCCAGCA GCCCAGCACT
    TCTCAGGGGT CACAGGTCAC TGATGCCACA GAATCTCTGG ATTTTGAGAC TCTGCCAGAG
    GATTCTGGAT CTGAGGTGGT ACCCGAGACA CCACCCCACA GCCGGCCTCA GGAAGACGAA
    GGGTCAGATC CCAGCGAACC CAGCACAGAC AGCACAGGTC AGGCTGAATA TGTTTCTCGT
    ATCCGAAGGT TAATGGCTGA GGGAGGCATG ACTGCGGTTG TCCAGCGTGA GCAGAGCACC
    ACAATGGCGT CCATGGGCAG CTTTGGCAAC AATATCATCG TCAGCCATCG CATCCACCGA
    GGCTCCCAGA CAGGAGCGGA TGCACAAAAT CGTACTCGTT TAAGTCCAAT ACCTGGTCCT
    TCTTCTGGTG CTCCGGAGTC TTTAGCTGCA GCTTCGTATT CGCGCGTCCT CACTAATACA
    TTAGGCTTTC GTGGCGACAC AGCTCAAGGC ATAGATCTCA CAGAACAGGA GAGACTGCAC
    ACATCCTTCT TTACACCGGA GTTCTCTCCT CTTTTCTCAT CTGCTGTCGA TGCAACCGGT
    CCTTCCTCCT CAATAGGAGC AGACAGCGTC CTGGAGGGAG AAGACTTTCA CGACTTTGCG
    TCTCTCCCTC CATCTTTACT CTCATCCTCG CCGTCTCTTT CACCAGTGAA TAACAGTAAC
    TACAGCAACA GCGATAGTAG TTATCTTGGA GATGAGTACG GGCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13301
    Clone ID Related Accession (Same CDS sequence) XM_002667669.5 , XM_002667669.6
    Accession Version XM_002667669.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002667669.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
    GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA
    TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT
    AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG
    GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA
    ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGACGG AGAGGTCAGA
    ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA
    TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT
    CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG
    AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC
    CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT
    CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC
    AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT
    GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG
    CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT
    GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC
    ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT
    GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC
    AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG
    GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC
    AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA
    CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC
    ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC
    TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA
    ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG
    CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC
    GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC
    TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC
    ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT
    GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA
    CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT
    AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA
    AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC
    TTGCGCAGGA GGCTGCTGGA GTCGTCCATG ATGTCTCTTT CGAGAAATGG CACCTCTGGG
    TCACGAGACC ACCCCATCTA TCCCGACCCA GCCAGGTTGT CCCCTGCAGC ATATTATGCA
    CAGCGTATGA TCCAGTATCT GTCTCGACGG GACAGCGTTC GGCAGCACTC CCACCGTCCC
    CCTAGCAGAC CTCGACCCCT GTCCTCTAAT CCCAGCAGTC TTTCCCCGAG CCCTGTCCCT
    AACGCAGAGA GCAGCGAAGT TGACTTTGAG GAGTTTGAGG AAAATGGAAG CAGATATCGG
    ACTCCTCGTA ATGCTCGCAT GTCTGCCCCC TCTCTTGGTC GTTTTGTTGG AACCAGGCGC
    TTTCTCTTGC CGGAGTTTTT GCCGTATGCT GGAATCTTCC ATGAGCGAGG GCAGCCAGGC
    CTGGCAACGC ACTCATCCGT CAATAGGGTC CTCGCAGGTG CTGTAATTGG AGACGGTCAA
    TCTGCAGTGG CCAGCAACAT CGCTAACACT ACCTATCGTC TGCAGTGGTG GGACTTCACC
    AAATTTGACT TGCCTGAAAT CAGTAATGCG TCGGTGAACG TTCTGGTGCC TAACTGTAAG
    ATCTACAACG ATGCGAGCTG TGATATCTCA GCGGATGGGC AGTTGCTGGC CGTATTCATT
    CCCAGCAGTC AGAGGGGTTT CCCTGATGAG GGCATTCTGG CTGTTTACTC TCTCGCTCCG
    CACAACCTGG GAGAGATGCT CTACTCCAAA AGATTCGGTC CAAATGCCAT CTCTGTCAGT
    TTGTCTCCGA TGGGTCGTTA CGTGATGGTG GGTCTGGCGT CTCGCCGCAT CCTGCTGCAT
    CAGATCAGCG ATCACATGGT GGCACAGGTC TTCAGACTGC AGCAGCCTCA CGCAGGAGAG
    ACGTCCATGA GGAGGGTGTT TGATGTGGTG TACCCAATGG CACCAGACCA GAGGAGACAC
    GTCAGTATAA ACTCTGCCCG CTGGCTGCCA GATCCGGGGC TTGGGCTCGC TTATGGGACC
    AACAAAGGAG ATCTTGTCAT CTGCAGACCT GTCGATGTGC ATTCTGATGG CAGCAGCACC
    TCTGAACACT CTGAGCGAAT GTTCACCATC AATAATGGTG GAGGGGTTGG ACCCAGTAGC
    AGCAGGAGTG GAGACAGGGC AGGGAGCAGC AGGACTGATC GACGGTCACG CAGAGACATA
    GGCCTGATGA ATGGAGTTGG TCTGCAGCCA CAACCTCCCG CCGCTAGTGT TACATCACAG
    GGGACGCAGA CACAGAACCA GCGACTACAG CATGCAGAGA CACAGACCGA CCGCGATCTT
    CCAGATGACC CCCAGCAGCC CAGCACTTCT CAGGGGTCAC AGGTCACTGA TGCCACAGAA
    TCTCTGGATT TTGAGACTCT GCCAGAGGAT TCTGGATCTG AGGTGGTACC CGAGACACCA
    CCCCACAGCC GGCCTCAGGA AGACGAAGGG TCAGATCCCA GCGAACCCAG CACAGACAGC
    ACAGGTCAGG CTGAATATGT TTCTCGTATC CGAAGGTTAA TGGCTGAGGG AGGCATGACT
    GCGGTTGTCC AGCGTGAGCA GAGCACCACA ATGGCGTCCA TGGGCAGCTT TGGCAACAAT
    ATCATCGTCA GCCATCGCAT CCACCGAGGC TCCCAGACAG GAGCGGATGC ACAAAATCGT
    ACTCGTTTAA GTCCAATACC TGGTCCTTCT TCTGGTGCTC CGGAGTCTTT AGCTGCAGCT
    TCGTATTCGC GCGTCCTCAC TAATACATTA GGCTTTCGTG GCGACACAGC TCAAGGCATA
    GATCTCACAG AACAGGAGAG ACTGCACACA TCCTTCTTTA CACCGGAGTT CTCTCCTCTT
    TTCTCATCTG CTGTCGATGC AACCGGTCCT TCCTCCTCAA TAGGAGCAGA CAGCGTCCTG
    GAGGGAGAAG ACTTTCACGA CTTTGCGTCT CTCCCTCCAT CTTTACTCTC ATCCTCGCCG
    TCTCTTTCAC CAGTGAATAA CAGTAACTAC AGCAACAGCG ATAGTAGTTA TCTTGGAGAT
    GAGTACGGGC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002667715.3
    CDS385..4419
    Translation

    Target ORF information:

    RefSeq Version XM_002667669.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002667669.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
    GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA
    TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT
    AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG
    GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA
    ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGACGG AGAGGTCAGA
    ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA
    TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT
    CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG
    AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC
    CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT
    CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC
    AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT
    GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG
    CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT
    GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC
    ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT
    GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC
    AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG
    GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC
    AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA
    CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC
    ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC
    TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA
    ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG
    CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC
    GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC
    TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC
    ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT
    GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA
    CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT
    AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA
    AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC
    TTGCGCAGGA GGCTGCTGGA GTCGTCCATG ATGTCTCTTT CGAGAAATGG CACCTCTGGG
    TCACGAGACC ACCCCATCTA TCCCGACCCA GCCAGGTTGT CCCCTGCAGC ATATTATGCA
    CAGCGTATGA TCCAGTATCT GTCTCGACGG GACAGCGTTC GGCAGCACTC CCACCGTCCC
    CCTAGCAGAC CTCGACCCCT GTCCTCTAAT CCCAGCAGTC TTTCCCCGAG CCCTGTCCCT
    AACGCAGAGA GCAGCGAAGT TGACTTTGAG GAGTTTGAGG AAAATGGAAG CAGATATCGG
    ACTCCTCGTA ATGCTCGCAT GTCTGCCCCC TCTCTTGGTC GTTTTGTTGG AACCAGGCGC
    TTTCTCTTGC CGGAGTTTTT GCCGTATGCT GGAATCTTCC ATGAGCGAGG GCAGCCAGGC
    CTGGCAACGC ACTCATCCGT CAATAGGGTC CTCGCAGGTG CTGTAATTGG AGACGGTCAA
    TCTGCAGTGG CCAGCAACAT CGCTAACACT ACCTATCGTC TGCAGTGGTG GGACTTCACC
    AAATTTGACT TGCCTGAAAT CAGTAATGCG TCGGTGAACG TTCTGGTGCC TAACTGTAAG
    ATCTACAACG ATGCGAGCTG TGATATCTCA GCGGATGGGC AGTTGCTGGC CGTATTCATT
    CCCAGCAGTC AGAGGGGTTT CCCTGATGAG GGCATTCTGG CTGTTTACTC TCTCGCTCCG
    CACAACCTGG GAGAGATGCT CTACTCCAAA AGATTCGGTC CAAATGCCAT CTCTGTCAGT
    TTGTCTCCGA TGGGTCGTTA CGTGATGGTG GGTCTGGCGT CTCGCCGCAT CCTGCTGCAT
    CAGATCAGCG ATCACATGGT GGCACAGGTC TTCAGACTGC AGCAGCCTCA CGCAGGAGAG
    ACGTCCATGA GGAGGGTGTT TGATGTGGTG TACCCAATGG CACCAGACCA GAGGAGACAC
    GTCAGTATAA ACTCTGCCCG CTGGCTGCCA GATCCGGGGC TTGGGCTCGC TTATGGGACC
    AACAAAGGAG ATCTTGTCAT CTGCAGACCT GTCGATGTGC ATTCTGATGG CAGCAGCACC
    TCTGAACACT CTGAGCGAAT GTTCACCATC AATAATGGTG GAGGGGTTGG ACCCAGTAGC
    AGCAGGAGTG GAGACAGGGC AGGGAGCAGC AGGACTGATC GACGGTCACG CAGAGACATA
    GGCCTGATGA ATGGAGTTGG TCTGCAGCCA CAACCTCCCG CCGCTAGTGT TACATCACAG
    GGGACGCAGA CACAGAACCA GCGACTACAG CATGCAGAGA CACAGACCGA CCGCGATCTT
    CCAGATGACC CCCAGCAGCC CAGCACTTCT CAGGGGTCAC AGGTCACTGA TGCCACAGAA
    TCTCTGGATT TTGAGACTCT GCCAGAGGAT TCTGGATCTG AGGTGGTACC CGAGACACCA
    CCCCACAGCC GGCCTCAGGA AGACGAAGGG TCAGATCCCA GCGAACCCAG CACAGACAGC
    ACAGGTCAGG CTGAATATGT TTCTCGTATC CGAAGGTTAA TGGCTGAGGG AGGCATGACT
    GCGGTTGTCC AGCGTGAGCA GAGCACCACA ATGGCGTCCA TGGGCAGCTT TGGCAACAAT
    ATCATCGTCA GCCATCGCAT CCACCGAGGC TCCCAGACAG GAGCGGATGC ACAAAATCGT
    ACTCGTTTAA GTCCAATACC TGGTCCTTCT TCTGGTGCTC CGGAGTCTTT AGCTGCAGCT
    TCGTATTCGC GCGTCCTCAC TAATACATTA GGCTTTCGTG GCGACACAGC TCAAGGCATA
    GATCTCACAG AACAGGAGAG ACTGCACACA TCCTTCTTTA CACCGGAGTT CTCTCCTCTT
    TTCTCATCTG CTGTCGATGC AACCGGTCCT TCCTCCTCAA TAGGAGCAGA CAGCGTCCTG
    GAGGGAGAAG ACTTTCACGA CTTTGCGTCT CTCCCTCCAT CTTTACTCTC ATCCTCGCCG
    TCTCTTTCAC CAGTGAATAA CAGTAACTAC AGCAACAGCG ATAGTAGTTA TCTTGGAGAT
    GAGTACGGGC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13301
    Clone ID Related Accession (Same CDS sequence) XM_002667669.5 , XM_002667669.6
    Accession Version XM_002667669.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002667669.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
    GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA
    TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT
    AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG
    GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA
    ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGACGG AGAGGTCAGA
    ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA
    TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT
    CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG
    AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC
    CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT
    CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC
    AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT
    GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG
    CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT
    GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC
    ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT
    GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC
    AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG
    GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC
    AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA
    CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC
    ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC
    TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA
    ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG
    CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC
    GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC
    TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC
    ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT
    GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA
    CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT
    AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA
    AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC
    TTGCGCAGGA GGCTGCTGGA GTCGTCCATG ATGTCTCTTT CGAGAAATGG CACCTCTGGG
    TCACGAGACC ACCCCATCTA TCCCGACCCA GCCAGGTTGT CCCCTGCAGC ATATTATGCA
    CAGCGTATGA TCCAGTATCT GTCTCGACGG GACAGCGTTC GGCAGCACTC CCACCGTCCC
    CCTAGCAGAC CTCGACCCCT GTCCTCTAAT CCCAGCAGTC TTTCCCCGAG CCCTGTCCCT
    AACGCAGAGA GCAGCGAAGT TGACTTTGAG GAGTTTGAGG AAAATGGAAG CAGATATCGG
    ACTCCTCGTA ATGCTCGCAT GTCTGCCCCC TCTCTTGGTC GTTTTGTTGG AACCAGGCGC
    TTTCTCTTGC CGGAGTTTTT GCCGTATGCT GGAATCTTCC ATGAGCGAGG GCAGCCAGGC
    CTGGCAACGC ACTCATCCGT CAATAGGGTC CTCGCAGGTG CTGTAATTGG AGACGGTCAA
    TCTGCAGTGG CCAGCAACAT CGCTAACACT ACCTATCGTC TGCAGTGGTG GGACTTCACC
    AAATTTGACT TGCCTGAAAT CAGTAATGCG TCGGTGAACG TTCTGGTGCC TAACTGTAAG
    ATCTACAACG ATGCGAGCTG TGATATCTCA GCGGATGGGC AGTTGCTGGC CGTATTCATT
    CCCAGCAGTC AGAGGGGTTT CCCTGATGAG GGCATTCTGG CTGTTTACTC TCTCGCTCCG
    CACAACCTGG GAGAGATGCT CTACTCCAAA AGATTCGGTC CAAATGCCAT CTCTGTCAGT
    TTGTCTCCGA TGGGTCGTTA CGTGATGGTG GGTCTGGCGT CTCGCCGCAT CCTGCTGCAT
    CAGATCAGCG ATCACATGGT GGCACAGGTC TTCAGACTGC AGCAGCCTCA CGCAGGAGAG
    ACGTCCATGA GGAGGGTGTT TGATGTGGTG TACCCAATGG CACCAGACCA GAGGAGACAC
    GTCAGTATAA ACTCTGCCCG CTGGCTGCCA GATCCGGGGC TTGGGCTCGC TTATGGGACC
    AACAAAGGAG ATCTTGTCAT CTGCAGACCT GTCGATGTGC ATTCTGATGG CAGCAGCACC
    TCTGAACACT CTGAGCGAAT GTTCACCATC AATAATGGTG GAGGGGTTGG ACCCAGTAGC
    AGCAGGAGTG GAGACAGGGC AGGGAGCAGC AGGACTGATC GACGGTCACG CAGAGACATA
    GGCCTGATGA ATGGAGTTGG TCTGCAGCCA CAACCTCCCG CCGCTAGTGT TACATCACAG
    GGGACGCAGA CACAGAACCA GCGACTACAG CATGCAGAGA CACAGACCGA CCGCGATCTT
    CCAGATGACC CCCAGCAGCC CAGCACTTCT CAGGGGTCAC AGGTCACTGA TGCCACAGAA
    TCTCTGGATT TTGAGACTCT GCCAGAGGAT TCTGGATCTG AGGTGGTACC CGAGACACCA
    CCCCACAGCC GGCCTCAGGA AGACGAAGGG TCAGATCCCA GCGAACCCAG CACAGACAGC
    ACAGGTCAGG CTGAATATGT TTCTCGTATC CGAAGGTTAA TGGCTGAGGG AGGCATGACT
    GCGGTTGTCC AGCGTGAGCA GAGCACCACA ATGGCGTCCA TGGGCAGCTT TGGCAACAAT
    ATCATCGTCA GCCATCGCAT CCACCGAGGC TCCCAGACAG GAGCGGATGC ACAAAATCGT
    ACTCGTTTAA GTCCAATACC TGGTCCTTCT TCTGGTGCTC CGGAGTCTTT AGCTGCAGCT
    TCGTATTCGC GCGTCCTCAC TAATACATTA GGCTTTCGTG GCGACACAGC TCAAGGCATA
    GATCTCACAG AACAGGAGAG ACTGCACACA TCCTTCTTTA CACCGGAGTT CTCTCCTCTT
    TTCTCATCTG CTGTCGATGC AACCGGTCCT TCCTCCTCAA TAGGAGCAGA CAGCGTCCTG
    GAGGGAGAAG ACTTTCACGA CTTTGCGTCT CTCCCTCCAT CTTTACTCTC ATCCTCGCCG
    TCTCTTTCAC CAGTGAATAA CAGTAACTAC AGCAACAGCG ATAGTAGTTA TCTTGGAGAT
    GAGTACGGGC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002667715.3
    CDS322..4356
    Translation

    Target ORF information:

    RefSeq Version XM_002667669.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio autophagy/beclin-1 regulator 1a (ambra1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002667669.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAGCTGG GACAGAGGAA CTCAGTATGT ATCCTGTCCA GTCGAGAGCG GGGAGCACCG 
    GGTCTTGCAT CATATAGGGT TCTGCAGCAG CTTGTAGAAG AGAAGACACA GCGAATGAAA
    TGGCAAAGTC AGAAAGTAGA GTTACCAGAC AGTCCCCGCT CAACCTTTCT GCTAGCCTTT
    AGTCCGGACA GGTCTTTAAT GGCTTCCACA CATGTGAACC ATAACATCTA CATCACTGAG
    GTTAAATCAG GAAAGTGTGT TCATTCATTG GTGGGCCATC GCAGGACACC CTGGTGTCTA
    ACTTTTCACC CTATCATCCC TGGGCTTATT GCTTCTGGCT GCCTGGACGG AGAGGTCAGA
    ATCTGGGACC TACATGGTGG CAGTGAAAGC TGGCTTACAG AGAGTAACTC CGCAATCGCA
    TCTCTTGCTT TCCACCCCAC GGCTCAGCTG CTGCTCATTG CCACCAACAA TGAGGTGCAT
    CTGTGGGACT GGAGTCGGAA AGAGCCCTTC ACTGTGGTCA AAACTGCCAG TGAGACAGAG
    AGAGTCAGAT TGGTGAGATT TGATCCATTG GGTCACTATC TTCTAACTGC CATTGTAAAC
    CCTTCGAACC AACCGAATGA TGATGATCCT GAGATTCCAA TGGACAGTGT GGAGATGCCT
    CATCTCCGAC AGCGCTCCTT CCTGCAGTCC CAGCCTGCGA GGCGCACACC CATCTTGCAC
    AATTTCTTGC ACATCCTCAC TTCCCGCAAC TCTGTTCCTC AGGCTGGAGG TGCCCATTCT
    GCTTCCACTG ATGGGTCCTC CGATTCTTCT GGCCCTTACA CTCTTATGTG CGTTCAGCCG
    CTTGGAATGG TGTGTTTTTG TAGCCGTTGC TCTGCAGCAC GAGTTCCATC TCCACCTGAT
    GAGGACCCTT CAGACTCGGC ATCCCTGGAG GCACAAGCTC ACACCTTTTC TTCTGCACGC
    ACAGAGCCAC TGCAGATGTC ACGGTTTTCT GTTGAGTCTC GTGCTGCAAA TCGCTCCTCT
    GCATTCAGCA GCGTGTATGG TGGAGGCAGC AACATGCGGA ATCATTCTTC CAGCTCTGGC
    AGGAGGGGAG TGACTGGAAT GGCACCTGTC CCGCATTTCC GACAACATCC ACCAGGTAGG
    GAAGGTGGAG GACGTCACCC TGGAGCAGAC TGGACAGTCA GTGGACTTAA TGGACAAAGC
    AGTAGTATGA CTCCACAAAG AACTGGCGCT TCCTCTGTTA GCCTTCTTTC TGTCCTGCGA
    CAGCAAGAGA CTTCCTTTCA ATCACCTGTT TACACTTCTG CTAGTGATAG ATGGGGCAGC
    ACACCGGGAA CTAGTTCCAG CAGGCACCGT CCTCCAGAAG AAGAGGGACA GAGCAGCTCC
    TCTTCCATCC ACAGTGTCCT GCGTTGTAAC CTTTACCGCT ATTTTATGGA TTATGAGGGA
    ACGCAGGACA CGGTCCAGCC TCTTGATGGC AGCAGACAGG ACCAGCAGAC TCAGGAAATG
    CTCAACAACA ACATGGACCC AGAGCAACCA GGACCATCTC ATTACCAGTC ACCCTATAGC
    GGTGAAAACC CTCCCCACAG TCACATGAAC CGCTGTAGAG TATGCCACAA TCTTTTCACC
    TACAATCAGG GTAGCCGGCG CTGGGACAGA ACAGGTCAGC CTTCATCTAC TGAGAGAAAC
    ACACCATGGC AACCTTCTAG TTCTGCCTTC CACAGCGTAG CTCCAGTTTC ACAATCTAAT
    GAACACCTAC TGGAACACAG ACCCATAGAG TCCACACCAA ATACCCCAGA GCCTCATGTA
    CCATTTTCCC AGAGAACTGA CACTGGTCAG CATGAGGAGC AGGCCGTTGG GCTTGTCTTT
    AACCAGGAAA CTGGCCAACT GGAGCGTGTT TACAGACAGT CTGCCTCCTC TCGCTCTGCA
    AACATATCAC AAGGAGCCTT AAACCAGGAA ATGCCTGAAG ACACACCAGA TAACGATTAC
    TTGCGCAGGA GGCTGCTGGA GTCGTCCATG ATGTCTCTTT CGAGAAATGG CACCTCTGGG
    TCACGAGACC ACCCCATCTA TCCCGACCCA GCCAGGTTGT CCCCTGCAGC ATATTATGCA
    CAGCGTATGA TCCAGTATCT GTCTCGACGG GACAGCGTTC GGCAGCACTC CCACCGTCCC
    CCTAGCAGAC CTCGACCCCT GTCCTCTAAT CCCAGCAGTC TTTCCCCGAG CCCTGTCCCT
    AACGCAGAGA GCAGCGAAGT TGACTTTGAG GAGTTTGAGG AAAATGGAAG CAGATATCGG
    ACTCCTCGTA ATGCTCGCAT GTCTGCCCCC TCTCTTGGTC GTTTTGTTGG AACCAGGCGC
    TTTCTCTTGC CGGAGTTTTT GCCGTATGCT GGAATCTTCC ATGAGCGAGG GCAGCCAGGC
    CTGGCAACGC ACTCATCCGT CAATAGGGTC CTCGCAGGTG CTGTAATTGG AGACGGTCAA
    TCTGCAGTGG CCAGCAACAT CGCTAACACT ACCTATCGTC TGCAGTGGTG GGACTTCACC
    AAATTTGACT TGCCTGAAAT CAGTAATGCG TCGGTGAACG TTCTGGTGCC TAACTGTAAG
    ATCTACAACG ATGCGAGCTG TGATATCTCA GCGGATGGGC AGTTGCTGGC CGTATTCATT
    CCCAGCAGTC AGAGGGGTTT CCCTGATGAG GGCATTCTGG CTGTTTACTC TCTCGCTCCG
    CACAACCTGG GAGAGATGCT CTACTCCAAA AGATTCGGTC CAAATGCCAT CTCTGTCAGT
    TTGTCTCCGA TGGGTCGTTA CGTGATGGTG GGTCTGGCGT CTCGCCGCAT CCTGCTGCAT
    CAGATCAGCG ATCACATGGT GGCACAGGTC TTCAGACTGC AGCAGCCTCA CGCAGGAGAG
    ACGTCCATGA GGAGGGTGTT TGATGTGGTG TACCCAATGG CACCAGACCA GAGGAGACAC
    GTCAGTATAA ACTCTGCCCG CTGGCTGCCA GATCCGGGGC TTGGGCTCGC TTATGGGACC
    AACAAAGGAG ATCTTGTCAT CTGCAGACCT GTCGATGTGC ATTCTGATGG CAGCAGCACC
    TCTGAACACT CTGAGCGAAT GTTCACCATC AATAATGGTG GAGGGGTTGG ACCCAGTAGC
    AGCAGGAGTG GAGACAGGGC AGGGAGCAGC AGGACTGATC GACGGTCACG CAGAGACATA
    GGCCTGATGA ATGGAGTTGG TCTGCAGCCA CAACCTCCCG CCGCTAGTGT TACATCACAG
    GGGACGCAGA CACAGAACCA GCGACTACAG CATGCAGAGA CACAGACCGA CCGCGATCTT
    CCAGATGACC CCCAGCAGCC CAGCACTTCT CAGGGGTCAC AGGTCACTGA TGCCACAGAA
    TCTCTGGATT TTGAGACTCT GCCAGAGGAT TCTGGATCTG AGGTGGTACC CGAGACACCA
    CCCCACAGCC GGCCTCAGGA AGACGAAGGG TCAGATCCCA GCGAACCCAG CACAGACAGC
    ACAGGTCAGG CTGAATATGT TTCTCGTATC CGAAGGTTAA TGGCTGAGGG AGGCATGACT
    GCGGTTGTCC AGCGTGAGCA GAGCACCACA ATGGCGTCCA TGGGCAGCTT TGGCAACAAT
    ATCATCGTCA GCCATCGCAT CCACCGAGGC TCCCAGACAG GAGCGGATGC ACAAAATCGT
    ACTCGTTTAA GTCCAATACC TGGTCCTTCT TCTGGTGCTC CGGAGTCTTT AGCTGCAGCT
    TCGTATTCGC GCGTCCTCAC TAATACATTA GGCTTTCGTG GCGACACAGC TCAAGGCATA
    GATCTCACAG AACAGGAGAG ACTGCACACA TCCTTCTTTA CACCGGAGTT CTCTCCTCTT
    TTCTCATCTG CTGTCGATGC AACCGGTCCT TCCTCCTCAA TAGGAGCAGA CAGCGTCCTG
    GAGGGAGAAG ACTTTCACGA CTTTGCGTCT CTCCCTCCAT CTTTACTCTC ATCCTCGCCG
    TCTCTTTCAC CAGTGAATAA CAGTAACTAC AGCAACAGCG ATAGTAGTTA TCTTGGAGAT
    GAGTACGGGC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.