GAREML cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAREML
Entrez Gene ID	100331995
Full Name	GRB2 associated, regulator of MAPK1-like
Synonyms	si:ch211-161e1.3
General protein information	Preferred Names GRB2 associated, regulator of MAPK1-like Names GRB2-associated and regulator of MAPK protein 2 fam59b
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002665782.5	XP_002665828.1	GRB2-associated and regulator of MAPK protein 2
XM_002665782.4	XP_002665828.1	GRB2-associated and regulator of MAPK protein 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gareml	NP_001161351.1
Danio rerio (zebrafish)	gareml	XP_002665828.1
Canis lupus familiaris (dog)	GAREML	XP_003639628.1
Bos taurus (cattle)	FAM59B	XP_002691549.1
Xenopus tropicalis (tropical clawed frog)	gareml	XP_004915027.1
Rattus norvegicus (Norway rat)	Gareml	NP_001178803.1
	LOC697774	XP_001086318.2
Gallus gallus (chicken)	FAM59B	XP_001234207.3
Homo sapiens (human)	GAREML	NP_001161713.1
Pan troglodytes (chimpanzee)	FAM59B	XP_003308961.1

Gene Ontology

The following GAREML gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAREML cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa25277	XM_002665782.5 Latest version!	Danio rerio GRB2 associated, regulator of MAPK1-like (gareml), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa25277	XM_002665782.4	Danio rerio GRB2 associated, regulator of MAPK1-like (gareml), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAREML ( XM_002665782.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002665828.1 Danio rerio GRB2 associated, regulator of MAPK1-like (gareml), mRNA.

Price & Availability↑

CloneID	ODa25277
Clone ID Related Accession (Same CDS sequence)	XM_002665782.5 , XM_002665782.4
Accession Version	XM_002665782.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	GRB2-associated and regulator of MAPK protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002665782.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TATCCGCGAG CATTACCGGA CTCTCGTGGA GCTCCGTGTC GCTGCCGCTG 
GACGTGATCG TGAGCAAATT CCGCTTACCG ACACTCGTGC GGCTGAGCAA CGGTGAGAAT 
GTAGAAGGTT TGTCAGAGGA GGACGTGATT CTGCTTCACT CCTGCAGACA GTGGACCACC 
GTCACTGCCC ACAGCCTGGA GGAGGGCCAT TACGTGATCG GACCCAAAAT AGACATCCCA 
TTACAGTATC AAGGGAAGTT TAAGTTATTG GATCAGGATC GGGATGTCCG TGAGCCGGTT 
CAGTATTTCG GAAGCGTGGA GGAAATCGCA GGAGTGTTTC CTGATCGCGT GTTCGTCATG 
GAACCCATCA CATTTAGTGT TAAGGTGGTT TCCGGGGAGT TCACAGAGGA CAGCGAGTTG 
TATAATTTCT CTCTGCAGGC GGGAGATGAA CTGACTCTTA TGGGCCAAGC AGAACTGCTC 
TGCGTCCAAT CAACTCGCGA GAAATCCCGC CTCACGGCGC TACTGCGCCG CCTGGGCAAA 
GCAGGAGGAG CACTGGGACG TCCAGCACGC ACGAAGATGC CTTGTTTAAT ATGCATGAAC 
CATCGAACCA ATGAGAGCGT TAGTTTGCCA TTCCAATGTC GTGGTCGGTT CTGCACGCGC 
TCACTGCAGG AGCTTCAGAT GCAGGGAGGC GAACACACCG TACGCAGCAT TATTGAACGC 
GTGCGGCTAC CGGTGAACGT TTCTGTTCCG TCACGACCTC CACGAAATCC GTACGACCTG 
CACGCCATCC GCGAGGGTCA TCGATATAAA CTTCTTAGCA TCATCAGCAA AACTGTTGTT 
CTCTGCTGCA TACTTCGCAA AGAGGAAGTG ATGCCTTCTC ACTTCCTGTT ATTAACGGAC 
ATGCCACGGT TTACACTTCC AGAGGGTCTT TTGCGCGCTG ATGCTCCCTA CCAGCAGGTG 
GTGCTACAGT GCGCACTGCG CTGCCAAGAA AGCTTCGATG CGGACGATTA TTCGCGTGCC 
GTACGTGAAG TCAAAACTGA TTTCTCAGAG GAATGTCTCA GTCCGAGGCG AATTCGGGTG 
TGTGTTCAGG GCTACGGGCG TGACGAGTTG GGGACATCCC TGCAAAGACT GTCTCTGTGT 
GTGTACGGAG GCGGAGCGGT CACGCACACA CACACAATCT CACACGGCTG CAGAGACTCA 
CTCGTCGACT CGCACACGCT GCCCTCTAGT GACGGCGATG GGGAAGAGCG AGAGTACGTC 
ACGCCTGATT GGTCGACAGA GTCGCAGGAG ATTCCATACG AGGAGCTCTG GACCAATCAG 
ACTTCTGGAA GTTTTGGAGT GACATCTGAG AGCGCAGTGA AGGCGGAGCA TAACCTCATA 
TCATTTCATT CCACTACTAC GTCATTGGAT GGAACGGTAG GCTCCACCCA CATCGCTATG 
GTGCAGATGG ATGCAGGAAG AGTGCCAAGG GTGTCGACCC CACCCCCTGT CCCACCAAAA 
TCTGAAGCTG TGAAAGAGGA GTGTCGCTTC TTAAATGCTC CTCCAGTCCC GCCCCGCTGT 
GCTAAAGGCC CCACCCCCAG CCCTCCAGTC CCCGCCCGCT TCCCTAAAAC TCCTCCCACA 
CAGACGCTCA ACCCAAATCT CTCCTTCTAT TCCTCTGGAC TGACAGAGAG CTCTGCTCCT 
CGTAGTGGCA GCAGCTCTCC ATCGCCAGAT TCTTACTCTC TGTACTGTTA CCCATGCACC 
TGGGCTGACT GCGTAACCTC TGACACCTGC GTGACCCCTG ACTCAACCCA GCCCGCACAG 
GCCTGTTGGT CCCACCCACG AGGAGGTGGA GCCTACACCT CCAACATCCT CAACACACCT 
TTACTCAGCA CTGACTCCAC CCAAAAGAAC TACTCCACTT GCCCAAGACC ACGACCAGTG 
GCACAAAACC GCTTCGCTCC TTTCGGCGCC CTAAACCCCT TCGCCAATCC AGGACACACC 
CACGCCTCCT CTGATTGGCT CACCACTGGT AGGGACTCTC CTACTCTAAT ACCTGACCTC 
ATAAGCTCCA CACCCAAGGA GACTCAAGCC AATCAGGGAG CTTTAAAAAG CAGTCCTGAA 
CCTCTTTTAA AACCGGTTAA AAGTTCAGAA GACACAGTCA CTATAGACAC CGCCTCTGGA 
TTGGTTGCCA TGGAAAGAGG GGCTGAGGGC GGGGCCGGGT CACCATGGCG ACCCCCCGCA 
GAGCTCTGCT GGCTCTCAGT GGAAGAGGTT TCATCCAGTC TGCGGTTTAT CGGCCTTTCG 
GACGACGTGA TTGGTCTGTT CAGCCGAGAG AGGATTGATG GCTCTATATT TACTCAGCTG 
ACAGAGGAAA TCCTGTCGGA GGACTTCAAA CTCACTAAAC TACAGGTCAA AAAAATCATG 
CAGTTCATTA AGGGATGGAG GCCCAAGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665828.1
CDS	145..2577
Translation	MEKLSASITGLSWSSVSLPLDVIVSKFRLPTLVRLSNGENVEGLSEEDVILLHSCRQWTTVTAHSLEEGHYVIGPKIDIPLQYQGKFKLLDQDRDVREPVQYFGSVEEIAGVFPDRVFVMEPITFSVKVVSGEFTEDSELYNFSLQAGDELTLMGQAELLCVQSTREKSRLTALLRRLGKAGGALGRPARTKMPCLICMNHRTNESVSLPFQCRGRFCTRSLQELQMQGGEHTVRSIIERVRLPVNVSVPSRPPRNPYDLHAIREGHRYKLLSIISKTVVLCCILRKEEVMPSHFLLLTDMPRFTLPEGLLRADAPYQQVVLQCALRCQESFDADDYSRAVREVKTDFSEECLSPRRIRVCVQGYGRDELGTSLQRLSLCVYGGGAVTHTHTISHGCRDSLVDSHTLPSSDGDGEEREYVTPDWSTESQEIPYEELWTNQTSGSFGVTSESAVKAEHNLISFHSTTTSLDGTVGSTHIAMVQMDAGRVPRVSTPPPVPPKSEAVKEECRFLNAPPVPPRCAKGPTPSPPVPARFPKTPPTQTLNPNLSFYSSGLTESSAPRSGSSSPSPDSYSLYCYPCTWADCVTSDTCVTPDSTQPAQACWSHPRGGGAYTSNILNTPLLSTDSTQKNYSTCPRPRPVAQNRFAPFGALNPFANPGHTHASSDWLTTGRDSPTLIPDLISSTPKETQANQGALKSSPEPLLKPVKSSEDTVTIDTASGLVAMERGAEGGAGSPWRPPAELCWLSVEEVSSSLRFIGLSDDVIGLFSRERIDGSIFTQLTEEILSEDFKLTKLQVKKIMQFIKGWRPKI

Target ORF information:

RefSeq Version	XM_002665782.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GRB2 associated, regulator of MAPK1-like (gareml), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665782.5

ORF Insert Sequence:

ATGGAGAAAC TATCCGCGAG CATTACCGGA CTCTCGTGGA GCTCCGTGTC GCTGCCGCTG 
GACGTGATCG TGAGCAAATT CCGCTTACCG ACACTCGTGC GGCTGAGCAA CGGTGAGAAT 
GTAGAAGGTT TGTCAGAGGA GGACGTGATT CTGCTTCACT CCTGCAGACA GTGGACCACC 
GTCACTGCCC ACAGCCTGGA GGAGGGCCAT TACGTGATCG GACCCAAAAT AGACATCCCA 
TTACAGTATC AAGGGAAGTT TAAGTTATTG GATCAGGATC GGGATGTCCG TGAGCCGGTT 
CAGTATTTCG GAAGCGTGGA GGAAATCGCA GGAGTGTTTC CTGATCGCGT GTTCGTCATG 
GAACCCATCA CATTTAGTGT TAAGGTGGTT TCCGGGGAGT TCACAGAGGA CAGCGAGTTG 
TATAATTTCT CTCTGCAGGC GGGAGATGAA CTGACTCTTA TGGGCCAAGC AGAACTGCTC 
TGCGTCCAAT CAACTCGCGA GAAATCCCGC CTCACGGCGC TACTGCGCCG CCTGGGCAAA 
GCAGGAGGAG CACTGGGACG TCCAGCACGC ACGAAGATGC CTTGTTTAAT ATGCATGAAC 
CATCGAACCA ATGAGAGCGT TAGTTTGCCA TTCCAATGTC GTGGTCGGTT CTGCACGCGC 
TCACTGCAGG AGCTTCAGAT GCAGGGAGGC GAACACACCG TACGCAGCAT TATTGAACGC 
GTGCGGCTAC CGGTGAACGT TTCTGTTCCG TCACGACCTC CACGAAATCC GTACGACCTG 
CACGCCATCC GCGAGGGTCA TCGATATAAA CTTCTTAGCA TCATCAGCAA AACTGTTGTT 
CTCTGCTGCA TACTTCGCAA AGAGGAAGTG ATGCCTTCTC ACTTCCTGTT ATTAACGGAC 
ATGCCACGGT TTACACTTCC AGAGGGTCTT TTGCGCGCTG ATGCTCCCTA CCAGCAGGTG 
GTGCTACAGT GCGCACTGCG CTGCCAAGAA AGCTTCGATG CGGACGATTA TTCGCGTGCC 
GTACGTGAAG TCAAAACTGA TTTCTCAGAG GAATGTCTCA GTCCGAGGCG AATTCGGGTG 
TGTGTTCAGG GCTACGGGCG TGACGAGTTG GGGACATCCC TGCAAAGACT GTCTCTGTGT 
GTGTACGGAG GCGGAGCGGT CACGCACACA CACACAATCT CACACGGCTG CAGAGACTCA 
CTCGTCGACT CGCACACGCT GCCCTCTAGT GACGGCGATG GGGAAGAGCG AGAGTACGTC 
ACGCCTGATT GGTCGACAGA GTCGCAGGAG ATTCCATACG AGGAGCTCTG GACCAATCAG 
ACTTCTGGAA GTTTTGGAGT GACATCTGAG AGCGCAGTGA AGGCGGAGCA TAACCTCATA 
TCATTTCATT CCACTACTAC GTCATTGGAT GGAACGGTAG GCTCCACCCA CATCGCTATG 
GTGCAGATGG ATGCAGGAAG AGTGCCAAGG GTGTCGACCC CACCCCCTGT CCCACCAAAA 
TCTGAAGCTG TGAAAGAGGA GTGTCGCTTC TTAAATGCTC CTCCAGTCCC GCCCCGCTGT 
GCTAAAGGCC CCACCCCCAG CCCTCCAGTC CCCGCCCGCT TCCCTAAAAC TCCTCCCACA 
CAGACGCTCA ACCCAAATCT CTCCTTCTAT TCCTCTGGAC TGACAGAGAG CTCTGCTCCT 
CGTAGTGGCA GCAGCTCTCC ATCGCCAGAT TCTTACTCTC TGTACTGTTA CCCATGCACC 
TGGGCTGACT GCGTAACCTC TGACACCTGC GTGACCCCTG ACTCAACCCA GCCCGCACAG 
GCCTGTTGGT CCCACCCACG AGGAGGTGGA GCCTACACCT CCAACATCCT CAACACACCT 
TTACTCAGCA CTGACTCCAC CCAAAAGAAC TACTCCACTT GCCCAAGACC ACGACCAGTG 
GCACAAAACC GCTTCGCTCC TTTCGGCGCC CTAAACCCCT TCGCCAATCC AGGACACACC 
CACGCCTCCT CTGATTGGCT CACCACTGGT AGGGACTCTC CTACTCTAAT ACCTGACCTC 
ATAAGCTCCA CACCCAAGGA GACTCAAGCC AATCAGGGAG CTTTAAAAAG CAGTCCTGAA 
CCTCTTTTAA AACCGGTTAA AAGTTCAGAA GACACAGTCA CTATAGACAC CGCCTCTGGA 
TTGGTTGCCA TGGAAAGAGG GGCTGAGGGC GGGGCCGGGT CACCATGGCG ACCCCCCGCA 
GAGCTCTGCT GGCTCTCAGT GGAAGAGGTT TCATCCAGTC TGCGGTTTAT CGGCCTTTCG 
GACGACGTGA TTGGTCTGTT CAGCCGAGAG AGGATTGATG GCTCTATATT TACTCAGCTG 
ACAGAGGAAA TCCTGTCGGA GGACTTCAAA CTCACTAAAC TACAGGTCAA AAAAATCATG 
CAGTTCATTA AGGGATGGAG GCCCAAGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREML ( XM_002665782.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002665828.1 Danio rerio GRB2 associated, regulator of MAPK1-like (gareml), mRNA.

Price & Availability↑

CloneID	ODa25277
Clone ID Related Accession (Same CDS sequence)	XM_002665782.5 , XM_002665782.4
Accession Version	XM_002665782.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	GRB2-associated and regulator of MAPK protein 2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002665782.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAC TATCCGCGAG CATTACCGGA CTCTCGTGGA GCTCCGTGTC GCTGCCGCTG 
GACGTGATCG TGAGCAAATT CCGCTTACCG ACACTCGTGC GGCTGAGCAA CGGTGAGAAT 
GTAGAAGGTT TGTCAGAGGA GGACGTGATT CTGCTTCACT CCTGCAGACA GTGGACCACC 
GTCACTGCCC ACAGCCTGGA GGAGGGCCAT TACGTGATCG GACCCAAAAT AGACATCCCA 
TTACAGTATC AAGGGAAGTT TAAGTTATTG GATCAGGATC GGGATGTCCG TGAGCCGGTT 
CAGTATTTCG GAAGCGTGGA GGAAATCGCA GGAGTGTTTC CTGATCGCGT GTTCGTCATG 
GAACCCATCA CATTTAGTGT TAAGGTGGTT TCCGGGGAGT TCACAGAGGA CAGCGAGTTG 
TATAATTTCT CTCTGCAGGC GGGAGATGAA CTGACTCTTA TGGGCCAAGC AGAACTGCTC 
TGCGTCCAAT CAACTCGCGA GAAATCCCGC CTCACGGCGC TACTGCGCCG CCTGGGCAAA 
GCAGGAGGAG CACTGGGACG TCCAGCACGC ACGAAGATGC CTTGTTTAAT ATGCATGAAC 
CATCGAACCA ATGAGAGCGT TAGTTTGCCA TTCCAATGTC GTGGTCGGTT CTGCACGCGC 
TCACTGCAGG AGCTTCAGAT GCAGGGAGGC GAACACACCG TACGCAGCAT TATTGAACGC 
GTGCGGCTAC CGGTGAACGT TTCTGTTCCG TCACGACCTC CACGAAATCC GTACGACCTG 
CACGCCATCC GCGAGGGTCA TCGATATAAA CTTCTTAGCA TCATCAGCAA AACTGTTGTT 
CTCTGCTGCA TACTTCGCAA AGAGGAAGTG ATGCCTTCTC ACTTCCTGTT ATTAACGGAC 
ATGCCACGGT TTACACTTCC AGAGGGTCTT TTGCGCGCTG ATGCTCCCTA CCAGCAGGTG 
GTGCTACAGT GCGCACTGCG CTGCCAAGAA AGCTTCGATG CGGACGATTA TTCGCGTGCC 
GTACGTGAAG TCAAAACTGA TTTCTCAGAG GAATGTCTCA GTCCGAGGCG AATTCGGGTG 
TGTGTTCAGG GCTACGGGCG TGACGAGTTG GGGACATCCC TGCAAAGACT GTCTCTGTGT 
GTGTACGGAG GCGGAGCGGT CACGCACACA CACACAATCT CACACGGCTG CAGAGACTCA 
CTCGTCGACT CGCACACGCT GCCCTCTAGT GACGGCGATG GGGAAGAGCG AGAGTACGTC 
ACGCCTGATT GGTCGACAGA GTCGCAGGAG ATTCCATACG AGGAGCTCTG GACCAATCAG 
ACTTCTGGAA GTTTTGGAGT GACATCTGAG AGCGCAGTGA AGGCGGAGCA TAACCTCATA 
TCATTTCATT CCACTACTAC GTCATTGGAT GGAACGGTAG GCTCCACCCA CATCGCTATG 
GTGCAGATGG ATGCAGGAAG AGTGCCAAGG GTGTCGACCC CACCCCCTGT CCCACCAAAA 
TCTGAAGCTG TGAAAGAGGA GTGTCGCTTC TTAAATGCTC CTCCAGTCCC GCCCCGCTGT 
GCTAAAGGCC CCACCCCCAG CCCTCCAGTC CCCGCCCGCT TCCCTAAAAC TCCTCCCACA 
CAGACGCTCA ACCCAAATCT CTCCTTCTAT TCCTCTGGAC TGACAGAGAG CTCTGCTCCT 
CGTAGTGGCA GCAGCTCTCC ATCGCCAGAT TCTTACTCTC TGTACTGTTA CCCATGCACC 
TGGGCTGACT GCGTAACCTC TGACACCTGC GTGACCCCTG ACTCAACCCA GCCCGCACAG 
GCCTGTTGGT CCCACCCACG AGGAGGTGGA GCCTACACCT CCAACATCCT CAACACACCT 
TTACTCAGCA CTGACTCCAC CCAAAAGAAC TACTCCACTT GCCCAAGACC ACGACCAGTG 
GCACAAAACC GCTTCGCTCC TTTCGGCGCC CTAAACCCCT TCGCCAATCC AGGACACACC 
CACGCCTCCT CTGATTGGCT CACCACTGGT AGGGACTCTC CTACTCTAAT ACCTGACCTC 
ATAAGCTCCA CACCCAAGGA GACTCAAGCC AATCAGGGAG CTTTAAAAAG CAGTCCTGAA 
CCTCTTTTAA AACCGGTTAA AAGTTCAGAA GACACAGTCA CTATAGACAC CGCCTCTGGA 
TTGGTTGCCA TGGAAAGAGG GGCTGAGGGC GGGGCCGGGT CACCATGGCG ACCCCCCGCA 
GAGCTCTGCT GGCTCTCAGT GGAAGAGGTT TCATCCAGTC TGCGGTTTAT CGGCCTTTCG 
GACGACGTGA TTGGTCTGTT CAGCCGAGAG AGGATTGATG GCTCTATATT TACTCAGCTG 
ACAGAGGAAA TCCTGTCGGA GGACTTCAAA CTCACTAAAC TACAGGTCAA AAAAATCATG 
CAGTTCATTA AGGGATGGAG GCCCAAGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665828.1
CDS	117..2549
Translation	MEKLSASITGLSWSSVSLPLDVIVSKFRLPTLVRLSNGENVEGLSEEDVILLHSCRQWTTVTAHSLEEGHYVIGPKIDIPLQYQGKFKLLDQDRDVREPVQYFGSVEEIAGVFPDRVFVMEPITFSVKVVSGEFTEDSELYNFSLQAGDELTLMGQAELLCVQSTREKSRLTALLRRLGKAGGALGRPARTKMPCLICMNHRTNESVSLPFQCRGRFCTRSLQELQMQGGEHTVRSIIERVRLPVNVSVPSRPPRNPYDLHAIREGHRYKLLSIISKTVVLCCILRKEEVMPSHFLLLTDMPRFTLPEGLLRADAPYQQVVLQCALRCQESFDADDYSRAVREVKTDFSEECLSPRRIRVCVQGYGRDELGTSLQRLSLCVYGGGAVTHTHTISHGCRDSLVDSHTLPSSDGDGEEREYVTPDWSTESQEIPYEELWTNQTSGSFGVTSESAVKAEHNLISFHSTTTSLDGTVGSTHIAMVQMDAGRVPRVSTPPPVPPKSEAVKEECRFLNAPPVPPRCAKGPTPSPPVPARFPKTPPTQTLNPNLSFYSSGLTESSAPRSGSSSPSPDSYSLYCYPCTWADCVTSDTCVTPDSTQPAQACWSHPRGGGAYTSNILNTPLLSTDSTQKNYSTCPRPRPVAQNRFAPFGALNPFANPGHTHASSDWLTTGRDSPTLIPDLISSTPKETQANQGALKSSPEPLLKPVKSSEDTVTIDTASGLVAMERGAEGGAGSPWRPPAELCWLSVEEVSSSLRFIGLSDDVIGLFSRERIDGSIFTQLTEEILSEDFKLTKLQVKKIMQFIKGWRPKI

Target ORF information:

RefSeq Version	XM_002665782.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio GRB2 associated, regulator of MAPK1-like (gareml), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665782.4

ORF Insert Sequence:

ATGGAGAAAC TATCCGCGAG CATTACCGGA CTCTCGTGGA GCTCCGTGTC GCTGCCGCTG 
GACGTGATCG TGAGCAAATT CCGCTTACCG ACACTCGTGC GGCTGAGCAA CGGTGAGAAT 
GTAGAAGGTT TGTCAGAGGA GGACGTGATT CTGCTTCACT CCTGCAGACA GTGGACCACC 
GTCACTGCCC ACAGCCTGGA GGAGGGCCAT TACGTGATCG GACCCAAAAT AGACATCCCA 
TTACAGTATC AAGGGAAGTT TAAGTTATTG GATCAGGATC GGGATGTCCG TGAGCCGGTT 
CAGTATTTCG GAAGCGTGGA GGAAATCGCA GGAGTGTTTC CTGATCGCGT GTTCGTCATG 
GAACCCATCA CATTTAGTGT TAAGGTGGTT TCCGGGGAGT TCACAGAGGA CAGCGAGTTG 
TATAATTTCT CTCTGCAGGC GGGAGATGAA CTGACTCTTA TGGGCCAAGC AGAACTGCTC 
TGCGTCCAAT CAACTCGCGA GAAATCCCGC CTCACGGCGC TACTGCGCCG CCTGGGCAAA 
GCAGGAGGAG CACTGGGACG TCCAGCACGC ACGAAGATGC CTTGTTTAAT ATGCATGAAC 
CATCGAACCA ATGAGAGCGT TAGTTTGCCA TTCCAATGTC GTGGTCGGTT CTGCACGCGC 
TCACTGCAGG AGCTTCAGAT GCAGGGAGGC GAACACACCG TACGCAGCAT TATTGAACGC 
GTGCGGCTAC CGGTGAACGT TTCTGTTCCG TCACGACCTC CACGAAATCC GTACGACCTG 
CACGCCATCC GCGAGGGTCA TCGATATAAA CTTCTTAGCA TCATCAGCAA AACTGTTGTT 
CTCTGCTGCA TACTTCGCAA AGAGGAAGTG ATGCCTTCTC ACTTCCTGTT ATTAACGGAC 
ATGCCACGGT TTACACTTCC AGAGGGTCTT TTGCGCGCTG ATGCTCCCTA CCAGCAGGTG 
GTGCTACAGT GCGCACTGCG CTGCCAAGAA AGCTTCGATG CGGACGATTA TTCGCGTGCC 
GTACGTGAAG TCAAAACTGA TTTCTCAGAG GAATGTCTCA GTCCGAGGCG AATTCGGGTG 
TGTGTTCAGG GCTACGGGCG TGACGAGTTG GGGACATCCC TGCAAAGACT GTCTCTGTGT 
GTGTACGGAG GCGGAGCGGT CACGCACACA CACACAATCT CACACGGCTG CAGAGACTCA 
CTCGTCGACT CGCACACGCT GCCCTCTAGT GACGGCGATG GGGAAGAGCG AGAGTACGTC 
ACGCCTGATT GGTCGACAGA GTCGCAGGAG ATTCCATACG AGGAGCTCTG GACCAATCAG 
ACTTCTGGAA GTTTTGGAGT GACATCTGAG AGCGCAGTGA AGGCGGAGCA TAACCTCATA 
TCATTTCATT CCACTACTAC GTCATTGGAT GGAACGGTAG GCTCCACCCA CATCGCTATG 
GTGCAGATGG ATGCAGGAAG AGTGCCAAGG GTGTCGACCC CACCCCCTGT CCCACCAAAA 
TCTGAAGCTG TGAAAGAGGA GTGTCGCTTC TTAAATGCTC CTCCAGTCCC GCCCCGCTGT 
GCTAAAGGCC CCACCCCCAG CCCTCCAGTC CCCGCCCGCT TCCCTAAAAC TCCTCCCACA 
CAGACGCTCA ACCCAAATCT CTCCTTCTAT TCCTCTGGAC TGACAGAGAG CTCTGCTCCT 
CGTAGTGGCA GCAGCTCTCC ATCGCCAGAT TCTTACTCTC TGTACTGTTA CCCATGCACC 
TGGGCTGACT GCGTAACCTC TGACACCTGC GTGACCCCTG ACTCAACCCA GCCCGCACAG 
GCCTGTTGGT CCCACCCACG AGGAGGTGGA GCCTACACCT CCAACATCCT CAACACACCT 
TTACTCAGCA CTGACTCCAC CCAAAAGAAC TACTCCACTT GCCCAAGACC ACGACCAGTG 
GCACAAAACC GCTTCGCTCC TTTCGGCGCC CTAAACCCCT TCGCCAATCC AGGACACACC 
CACGCCTCCT CTGATTGGCT CACCACTGGT AGGGACTCTC CTACTCTAAT ACCTGACCTC 
ATAAGCTCCA CACCCAAGGA GACTCAAGCC AATCAGGGAG CTTTAAAAAG CAGTCCTGAA 
CCTCTTTTAA AACCGGTTAA AAGTTCAGAA GACACAGTCA CTATAGACAC CGCCTCTGGA 
TTGGTTGCCA TGGAAAGAGG GGCTGAGGGC GGGGCCGGGT CACCATGGCG ACCCCCCGCA 
GAGCTCTGCT GGCTCTCAGT GGAAGAGGTT TCATCCAGTC TGCGGTTTAT CGGCCTTTCG 
GACGACGTGA TTGGTCTGTT CAGCCGAGAG AGGATTGATG GCTCTATATT TACTCAGCTG 
ACAGAGGAAA TCCTGTCGGA GGACTTCAAA CTCACTAAAC TACAGGTCAA AAAAATCATG 
CAGTTCATTA AGGGATGGAG GCCCAAGATA TGA