ITCHA cDNA ORF clone, Danio rerio(Zebrafish)

The following ITCHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITCHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa02213 NM_001287095.1
Latest version!
Danio rerio itchy E3 ubiquitin protein ligase a (itcha), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa49448 XM_009302759.3
Latest version!
Danio rerio itchy E3 ubiquitin protein ligase a (itcha), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
ODa49448 XM_009302759.2 Danio rerio itchy E3 ubiquitin protein ligase a (itcha), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa02213
    Clone ID Related Accession (Same CDS sequence) NM_001287095.1
    Accession Version NM_001287095.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product itchy E3 ubiquitin protein ligase a
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB863704.1. On Nov 26, 2013 this sequence version replaced XM_002667532.3. ##Evidence-Data-START## Transcript exon combination :: AB863704.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGGCTC AACTTCAGAT AACAGTTCTG TCTGCTAAGC TGCGGGAGAA TAAGAAGAAC 
    TGGTTTGGGC CCAGTCCTTA TGTGGAGGTG TTTGTGGACG GACAGTCGAA GAAGACGGAG
    AAATGCACCA ACACCCACAG TCCCAAATGG AAACAGTCTC TCACTGTGAT CGTCACTCCT
    TTCAGCAAGC TAATTTTCCG GGTTTGGAGC CACCAGACAC TAAAAGCGGA CATTTTGCTT
    GGCGTGGCCA GTCTGGAGGT CAGCGAGACC TTAAAAGCCA ACAACATGAA GATCTGTGAG
    GTGGTGCAGA CGCTGCAGTT GAGCTCAGAC AGAGGGCGCT CGGAGCAGGT TGGAGATCTG
    TCTTTGTGTC TGGATGGACT TCAGGTGGAT GCAGAGACTT TCGCTTCAGA GGAACAAAAG
    CACAATGCTG TTGTTAATGG AGAATCCAGA CTGAACGGGG ATGCCATAAG AAGTCGAGAC
    TCTTCTCCAT CTAGTGACAT CACTGACGAT GCGCCTTTGC CCAATGGCCA TGCAGTGGGA
    AGCTCCGCCT CTGCATCTCC ATCCACTGGG GCTTTGAGAG CACCGCGTCC CCTTCGACCC
    CAGAGACCAC CGCCTGCTTC ACCACATAAA CATACTTCCT CCACAGCATC CTCTACTGGC
    TCGACTCCCA CTCACGGCAG CAGCGCTCCC AGTCCAGAGA TGTCTCCACG AGTGTGTGTG
    AATGGAGAAA CCGAGAGTCA AGGGTCAAGC TCGGGCTCAG GCTCAAATCA GGCACCCAGA
    GCCAGTCCAA CACCACCCAG AGCTCCAGCC ATCACCAACG GCCCTCTTCC TCCAGGCTGG
    GAGCAGAGAG CGGATCAGAA CGGACGCGTT TACTATGTTG ATCACATCGA GAAAAGAACC
    ACATGGGAAA GACCTGAACC TCTGCCACCA GGTTGGGAGC GACGTTTAGA CCCAATGGGC
    CGTGTGTATT ATGTGGATCA TATAAACAGA ACCACCACAT GGCAGAGACC CACGCTAGAG
    TCGGTGCGCA ACTATGAGGA ATGGCAGAAC CAGCGCAGTC AACTTCAGGG AGCCATGCAG
    CGCTTCAACC AGAGGTTCAT CTATGGCCTG CAGGAACAGC TCGCGCCGAC AGCTAATAAA
    GAGTTTGACC CTCTGGGACC GCTGCCGTCA GGATGGGAGA AACGTACCGA CAGTAATAGG
    AGGATGTATT TTGTTCACCA TCCTACAAGA TCCACACAAT GGGAAGACCC TCGCACACAA
    GGGTTGCTAA ATGAGAAGCC TTTGCCGGAA GGTTGGGAAA TGAGGTTTAC AGTGGATGGG
    ATTCCCTATT TTGTGGATCA CAACAGAAGA ACTACAACAT ACATAGACCC CCGTACAGGA
    AAATCCTCGC TTGAGAACGG CCCTCAGATC ACATACGTAC GAGATTTTAA AGCCAAAGTG
    CACTATTTCC GCTTCTGGTG TCAGCAATTA TCAATGCCTC AACACATCAA AATCCATGTG
    AGTCGCAAAA CGCTGTTTGA GGACTCATTT CAGCAGATTA TGGGTTGCCA TCCACAAGAC
    CTGAGGCGAA GATTGTGGAT CATTTTCCCA GGGGAGGAGG GGCTTGACTA TGGAGGCGTG
    GCCAGGGAAT GGTTTTTCCT GCTCTCGCAT GAGGTCCTGA ACCCCATGTA CTGTCTGTTT
    GAATACGCCG GCAAAGATAA CTACTGCCTA CAGATTAACC CAGCATCCTC CATCAACCCA
    GACCACCTCA AATACTTCAA GTTCATCGGC CGCTTCATTG CTATGGCTTT GTTTCATGGG
    AAGTTCATCG ACACCGGCTT CTCTTTGCCC TTTTATAAGC GCATCCTGAA CAAACCTTTA
    GCTCTGAAGG ACCTGGAGTC CATCGACCCC GAGTTTTACA ACTCTCTCAT CTGGATCAAG
    GATAATAATA TCGAGGAGTG TGGCTTGGAG ATGTTTTTCT CTGTGGATAA GGAGATTCTG
    GGAGAGGTCA CAACTCATGA ACTCAAACCT GATGGAGGAA ACATTCAGGT GACGGAGGAA
    AATAAAGAGG AGTATATCAG GTTGGTTGCT GAATGGAGGT TGTCCAGAGG AGTCGAGGAG
    CAAACTCAGG CCTTCCTCGA GGGCTTTAAT GATGTCCTGC CGCAGCAGTA CCTGCAATAC
    TTTGACGCCA AAGAACTTGA GGTTATGCTG TGTGGGATGC AGGAGATCGA TCTGGGCGAC
    TGGCAGAGGA ACTCTATCTA CAGACACTAC GCTCGCAGCA GCAAACAGAT CGTCTGGTTC
    TGGCAGTTTG TGAAGGAGAT GGACAATGAA AAGCGCATGC GTCTGCTGCA GTTTGTGACC
    GGCACATGCA GACTTCCCGT TGGGGGATTC GCTGATCTTA TGGGCAGCAA CGGACCGCAA
    AAGTTTTGCA TTGAAAAAGT GGGAAAAGAA AACTGGCTGC CGAGGAGCCA CACATGCTTT
    AACCGTCTAG ACCTGCCGCC CTATAAAAGC TATGAACAGT TAAAGGAGAA GCTGCTCTTC
    GCCATCGAGG AAACAGAAGG TTTCGGTCAG GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001274024.1
    CDS82..2637
    Translation

    Target ORF information:

    RefSeq Version NM_001287095.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio itchy E3 ubiquitin protein ligase a (itcha), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001287095.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGGCTC AACTTCAGAT AACAGTTCTG TCTGCTAAGC TGCGGGAGAA TAAGAAGAAC 
    TGGTTTGGGC CCAGTCCTTA TGTGGAGGTG TTTGTGGACG GACAGTCGAA GAAGACGGAG
    AAATGCACCA ACACCCACAG TCCCAAATGG AAACAGTCTC TCACTGTGAT CGTCACTCCT
    TTCAGCAAGC TAATTTTCCG GGTTTGGAGC CACCAGACAC TAAAAGCGGA CATTTTGCTT
    GGCGTGGCCA GTCTGGAGGT CAGCGAGACC TTAAAAGCCA ACAACATGAA GATCTGTGAG
    GTGGTGCAGA CGCTGCAGTT GAGCTCAGAC AGAGGGCGCT CGGAGCAGGT TGGAGATCTG
    TCTTTGTGTC TGGATGGACT TCAGGTGGAT GCAGAGACTT TCGCTTCAGA GGAACAAAAG
    CACAATGCTG TTGTTAATGG AGAATCCAGA CTGAACGGGG ATGCCATAAG AAGTCGAGAC
    TCTTCTCCAT CTAGTGACAT CACTGACGAT GCGCCTTTGC CCAATGGCCA TGCAGTGGGA
    AGCTCCGCCT CTGCATCTCC ATCCACTGGG GCTTTGAGAG CACCGCGTCC CCTTCGACCC
    CAGAGACCAC CGCCTGCTTC ACCACATAAA CATACTTCCT CCACAGCATC CTCTACTGGC
    TCGACTCCCA CTCACGGCAG CAGCGCTCCC AGTCCAGAGA TGTCTCCACG AGTGTGTGTG
    AATGGAGAAA CCGAGAGTCA AGGGTCAAGC TCGGGCTCAG GCTCAAATCA GGCACCCAGA
    GCCAGTCCAA CACCACCCAG AGCTCCAGCC ATCACCAACG GCCCTCTTCC TCCAGGCTGG
    GAGCAGAGAG CGGATCAGAA CGGACGCGTT TACTATGTTG ATCACATCGA GAAAAGAACC
    ACATGGGAAA GACCTGAACC TCTGCCACCA GGTTGGGAGC GACGTTTAGA CCCAATGGGC
    CGTGTGTATT ATGTGGATCA TATAAACAGA ACCACCACAT GGCAGAGACC CACGCTAGAG
    TCGGTGCGCA ACTATGAGGA ATGGCAGAAC CAGCGCAGTC AACTTCAGGG AGCCATGCAG
    CGCTTCAACC AGAGGTTCAT CTATGGCCTG CAGGAACAGC TCGCGCCGAC AGCTAATAAA
    GAGTTTGACC CTCTGGGACC GCTGCCGTCA GGATGGGAGA AACGTACCGA CAGTAATAGG
    AGGATGTATT TTGTTCACCA TCCTACAAGA TCCACACAAT GGGAAGACCC TCGCACACAA
    GGGTTGCTAA ATGAGAAGCC TTTGCCGGAA GGTTGGGAAA TGAGGTTTAC AGTGGATGGG
    ATTCCCTATT TTGTGGATCA CAACAGAAGA ACTACAACAT ACATAGACCC CCGTACAGGA
    AAATCCTCGC TTGAGAACGG CCCTCAGATC ACATACGTAC GAGATTTTAA AGCCAAAGTG
    CACTATTTCC GCTTCTGGTG TCAGCAATTA TCAATGCCTC AACACATCAA AATCCATGTG
    AGTCGCAAAA CGCTGTTTGA GGACTCATTT CAGCAGATTA TGGGTTGCCA TCCACAAGAC
    CTGAGGCGAA GATTGTGGAT CATTTTCCCA GGGGAGGAGG GGCTTGACTA TGGAGGCGTG
    GCCAGGGAAT GGTTTTTCCT GCTCTCGCAT GAGGTCCTGA ACCCCATGTA CTGTCTGTTT
    GAATACGCCG GCAAAGATAA CTACTGCCTA CAGATTAACC CAGCATCCTC CATCAACCCA
    GACCACCTCA AATACTTCAA GTTCATCGGC CGCTTCATTG CTATGGCTTT GTTTCATGGG
    AAGTTCATCG ACACCGGCTT CTCTTTGCCC TTTTATAAGC GCATCCTGAA CAAACCTTTA
    GCTCTGAAGG ACCTGGAGTC CATCGACCCC GAGTTTTACA ACTCTCTCAT CTGGATCAAG
    GATAATAATA TCGAGGAGTG TGGCTTGGAG ATGTTTTTCT CTGTGGATAA GGAGATTCTG
    GGAGAGGTCA CAACTCATGA ACTCAAACCT GATGGAGGAA ACATTCAGGT GACGGAGGAA
    AATAAAGAGG AGTATATCAG GTTGGTTGCT GAATGGAGGT TGTCCAGAGG AGTCGAGGAG
    CAAACTCAGG CCTTCCTCGA GGGCTTTAAT GATGTCCTGC CGCAGCAGTA CCTGCAATAC
    TTTGACGCCA AAGAACTTGA GGTTATGCTG TGTGGGATGC AGGAGATCGA TCTGGGCGAC
    TGGCAGAGGA ACTCTATCTA CAGACACTAC GCTCGCAGCA GCAAACAGAT CGTCTGGTTC
    TGGCAGTTTG TGAAGGAGAT GGACAATGAA AAGCGCATGC GTCTGCTGCA GTTTGTGACC
    GGCACATGCA GACTTCCCGT TGGGGGATTC GCTGATCTTA TGGGCAGCAA CGGACCGCAA
    AAGTTTTGCA TTGAAAAAGT GGGAAAAGAA AACTGGCTGC CGAGGAGCCA CACATGCTTT
    AACCGTCTAG ACCTGCCGCC CTATAAAAGC TATGAACAGT TAAAGGAGAA GCTGCTCTTC
    GCCATCGAGG AAACAGAAGG TTTCGGTCAG GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49448
    Clone ID Related Accession (Same CDS sequence) XM_009302759.3 , XM_009302759.2
    Accession Version XM_009302759.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product itchy E3 ubiquitin protein ligase a isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302759.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGGCTC AACTTCAGAT AACAGTTCTG TCTGCTAAGC TGCGGGAGAA TAAGAAGAAC 
    TGGTTTGGGC CCAGTCCTTA TGTGGAGGTG TTTGTGGACG GACAGTCGAA GAAGACGGAG
    AAATGCACCA ACACCCACAG TCCCAAATGG AAACAGTCTC TCACTGTGAT CGTCACTCCT
    TTCAGCAAGC TAATTTTCCG GGTTTGGAGC CACCAGACAC TAAAAGCGGA CATTTTGCTT
    GGCGTGGCCA GTCTGGAGGT CAGCGAGACC TTAAAAGCCA ACAACATGAA GATCTGTGAG
    GTGGTGCAGA CGCTGCAGTT GAGCTCAGAC AGAGGGCGCT CGGAGCAGGT TGGAGATCTG
    TCTTTGTGTC TGGATGGACT TCAGGTGGAT GCAGAGACTT TCGCTTCAGA GGAACAAAAG
    CACAATGCTG TTGTTAATGG AGAATCCAGA CTGAACGGGG ATGCCATAAG AAGTCGAGAC
    TCTTCTCCAT CTAGTGACAT CACTGACGAT GCGCCTTTGC CCAATGGCCA TGCAGTGGGA
    AGCTCCGCCT CTGCATCTCC ATCCACTGGG GCTTTGAGAG CACCGCGTCC CCTTCGACCC
    CAGAGACCAC CGCCTGCTTC ACCACATAAA CATACTTCCT CCACAGCATC CTCTACTGGC
    TCGACTCCCA CTCACGGCAG CAGCGCTCCC AGTCCAGAGA TGTCTCCACG AGTGTGTGTG
    AATGGAGAAA CCGAGAGTCA AGGGTCAAGC TCGGGCTCAG GCTCAAATCA GGCACCCAGA
    GCCAGTCCAA CACCACCCAG AGCTCCAGCC ATCACCAACG GCCCTCTTCC TCCAGGCTGG
    GAGCAGAGAG CGGATCAGAA CGGACGCGTT TACTATGTTG ATCACATCGA GAAAAGAACC
    ACATGGGAAA GACCTGAACC TCTGCCACCA GGAACCACCA CATGGCAGAG ACCCACGCTA
    GAGTCGGTGC GCAACTATGA GGAATGGCAG AACCAGCGCA GTCAACTTCA GGGAGCCATG
    CAGCGCTTCA ACCAGAGGTT CATCTATGGC CTGCAGGAAC AGCTCGCGCC GACAGCTAAT
    AAAGAGTTTG ACCCTCTGGG ACCGCTGCCG TCAGGATGGG AGAAACGTAC CGACAGTAAT
    AGGAGGATGT ATTTTGTTCA CCATCCTACA AGATCCACAC AATGGGAAGA CCCTCGCACA
    CAAGGGTTGC TAAATGAGAA GCCTTTGCCG GAAGGTTGGG AAATGAGGTT TACAGTGGAT
    GGGATTCCCT ATTTTGTGGA TCACAACAGA AGAACTACAA CATACATAGA CCCCCGTACA
    GGAAAATCCT CGCTTGAGAA CGGCCCTCAG ATCACATACG TACGAGATTT TAAAGCCAAA
    GTGCACTATT TCCGCTTCTG GTGTCAGCAA TTATCAATGC CTCAACACAT CAAAATCCAT
    GTGAGTCGCA AAACGCTGTT TGAGGACTCA TTTCAGCAGA TTATGGGTTG CCATCCACAA
    GACCTGAGGC GAAGATTGTG GATCATTTTC CCAGGGGAGG AGGGGCTTGA CTATGGAGGC
    GTGGCCAGGG AATGGTTTTT CCTGCTCTCG CATGAGGTCC TGAACCCCAT GTACTGTCTG
    TTTGAATACG CCGGCAAAGA TAACTACTGC CTACAGATTA ACCCAGCATC CTCCATCAAC
    CCAGACCACC TCAAATACTT CAAGTTCATC GGCCGCTTCA TTGCTATGGC TTTGTTTCAT
    GGGAAGTTCA TCGACACCGG CTTCTCTTTG CCCTTTTATA AGCGCATCCT GAACAAACCT
    TTAGCTCTGA AGGACCTGGA GTCCATCGAC CCCGAGTTTT ACAACTCTCT CATCTGGATC
    AAGGATAATA ATATCGAGGA GTGTGGCTTG GAGATGTTTT TCTCTGTGGA TAAGGAGATT
    CTGGGAGAGG TCACAACTCA TGAACTCAAA CCTGATGGAG GAAACATTCA GGTGACGGAG
    GAAAATAAAG AGGAGTATAT CAGGTTGGTT GCTGAATGGA GGTTGTCCAG AGGAGTCGAG
    GAGCAAACTC AGGCCTTCCT CGAGGGCTTT AATGATGTCC TGCCGCAGCA GTACCTGCAA
    TACTTTGACG CCAAAGAACT TGAGGTTATG CTGTGTGGGA TGCAGGAGAT CGATCTGGGC
    GACTGGCAGA GGAACTCTAT CTACAGACAC TACGCTCGCA GCAGCAAACA GATCGTCTGG
    TTCTGGCAGT TTGTGAAGGA GATGGACAAT GAAAAGCGCA TGCGTCTGCT GCAGTTTGTG
    ACCGGCACAT GCAGACTTCC CGTTGGGGGA TTCGCTGATC TTATGGGCAG CAACGGACCG
    CAAAAGTTTT GCATTGAAAA AGTGGGAAAA GAAAACTGGC TGCCGAGGAG CCACACATGC
    TTTAACCGTC TAGACCTGCC GCCCTATAAA AGCTATGAAC AGTTAAAGGA GAAGCTGCTC
    TTCGCCATCG AGGAAACAGA AGGTTTCGGT CAGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301034.1
    CDS192..2690
    Translation

    Target ORF information:

    RefSeq Version XM_009302759.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio itchy E3 ubiquitin protein ligase a (itcha), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302759.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGGCTC AACTTCAGAT AACAGTTCTG TCTGCTAAGC TGCGGGAGAA TAAGAAGAAC 
    TGGTTTGGGC CCAGTCCTTA TGTGGAGGTG TTTGTGGACG GACAGTCGAA GAAGACGGAG
    AAATGCACCA ACACCCACAG TCCCAAATGG AAACAGTCTC TCACTGTGAT CGTCACTCCT
    TTCAGCAAGC TAATTTTCCG GGTTTGGAGC CACCAGACAC TAAAAGCGGA CATTTTGCTT
    GGCGTGGCCA GTCTGGAGGT CAGCGAGACC TTAAAAGCCA ACAACATGAA GATCTGTGAG
    GTGGTGCAGA CGCTGCAGTT GAGCTCAGAC AGAGGGCGCT CGGAGCAGGT TGGAGATCTG
    TCTTTGTGTC TGGATGGACT TCAGGTGGAT GCAGAGACTT TCGCTTCAGA GGAACAAAAG
    CACAATGCTG TTGTTAATGG AGAATCCAGA CTGAACGGGG ATGCCATAAG AAGTCGAGAC
    TCTTCTCCAT CTAGTGACAT CACTGACGAT GCGCCTTTGC CCAATGGCCA TGCAGTGGGA
    AGCTCCGCCT CTGCATCTCC ATCCACTGGG GCTTTGAGAG CACCGCGTCC CCTTCGACCC
    CAGAGACCAC CGCCTGCTTC ACCACATAAA CATACTTCCT CCACAGCATC CTCTACTGGC
    TCGACTCCCA CTCACGGCAG CAGCGCTCCC AGTCCAGAGA TGTCTCCACG AGTGTGTGTG
    AATGGAGAAA CCGAGAGTCA AGGGTCAAGC TCGGGCTCAG GCTCAAATCA GGCACCCAGA
    GCCAGTCCAA CACCACCCAG AGCTCCAGCC ATCACCAACG GCCCTCTTCC TCCAGGCTGG
    GAGCAGAGAG CGGATCAGAA CGGACGCGTT TACTATGTTG ATCACATCGA GAAAAGAACC
    ACATGGGAAA GACCTGAACC TCTGCCACCA GGAACCACCA CATGGCAGAG ACCCACGCTA
    GAGTCGGTGC GCAACTATGA GGAATGGCAG AACCAGCGCA GTCAACTTCA GGGAGCCATG
    CAGCGCTTCA ACCAGAGGTT CATCTATGGC CTGCAGGAAC AGCTCGCGCC GACAGCTAAT
    AAAGAGTTTG ACCCTCTGGG ACCGCTGCCG TCAGGATGGG AGAAACGTAC CGACAGTAAT
    AGGAGGATGT ATTTTGTTCA CCATCCTACA AGATCCACAC AATGGGAAGA CCCTCGCACA
    CAAGGGTTGC TAAATGAGAA GCCTTTGCCG GAAGGTTGGG AAATGAGGTT TACAGTGGAT
    GGGATTCCCT ATTTTGTGGA TCACAACAGA AGAACTACAA CATACATAGA CCCCCGTACA
    GGAAAATCCT CGCTTGAGAA CGGCCCTCAG ATCACATACG TACGAGATTT TAAAGCCAAA
    GTGCACTATT TCCGCTTCTG GTGTCAGCAA TTATCAATGC CTCAACACAT CAAAATCCAT
    GTGAGTCGCA AAACGCTGTT TGAGGACTCA TTTCAGCAGA TTATGGGTTG CCATCCACAA
    GACCTGAGGC GAAGATTGTG GATCATTTTC CCAGGGGAGG AGGGGCTTGA CTATGGAGGC
    GTGGCCAGGG AATGGTTTTT CCTGCTCTCG CATGAGGTCC TGAACCCCAT GTACTGTCTG
    TTTGAATACG CCGGCAAAGA TAACTACTGC CTACAGATTA ACCCAGCATC CTCCATCAAC
    CCAGACCACC TCAAATACTT CAAGTTCATC GGCCGCTTCA TTGCTATGGC TTTGTTTCAT
    GGGAAGTTCA TCGACACCGG CTTCTCTTTG CCCTTTTATA AGCGCATCCT GAACAAACCT
    TTAGCTCTGA AGGACCTGGA GTCCATCGAC CCCGAGTTTT ACAACTCTCT CATCTGGATC
    AAGGATAATA ATATCGAGGA GTGTGGCTTG GAGATGTTTT TCTCTGTGGA TAAGGAGATT
    CTGGGAGAGG TCACAACTCA TGAACTCAAA CCTGATGGAG GAAACATTCA GGTGACGGAG
    GAAAATAAAG AGGAGTATAT CAGGTTGGTT GCTGAATGGA GGTTGTCCAG AGGAGTCGAG
    GAGCAAACTC AGGCCTTCCT CGAGGGCTTT AATGATGTCC TGCCGCAGCA GTACCTGCAA
    TACTTTGACG CCAAAGAACT TGAGGTTATG CTGTGTGGGA TGCAGGAGAT CGATCTGGGC
    GACTGGCAGA GGAACTCTAT CTACAGACAC TACGCTCGCA GCAGCAAACA GATCGTCTGG
    TTCTGGCAGT TTGTGAAGGA GATGGACAAT GAAAAGCGCA TGCGTCTGCT GCAGTTTGTG
    ACCGGCACAT GCAGACTTCC CGTTGGGGGA TTCGCTGATC TTATGGGCAG CAACGGACCG
    CAAAAGTTTT GCATTGAAAA AGTGGGAAAA GAAAACTGGC TGCCGAGGAG CCACACATGC
    TTTAACCGTC TAGACCTGCC GCCCTATAAA AGCTATGAAC AGTTAAAGGA GAAGCTGCTC
    TTCGCCATCG AGGAAACAGA AGGTTTCGGT CAGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49448
    Clone ID Related Accession (Same CDS sequence) XM_009302759.3 , XM_009302759.2
    Accession Version XM_009302759.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product itchy E3 ubiquitin protein ligase a isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302759.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGGCTC AACTTCAGAT AACAGTTCTG TCTGCTAAGC TGCGGGAGAA TAAGAAGAAC 
    TGGTTTGGGC CCAGTCCTTA TGTGGAGGTG TTTGTGGACG GACAGTCGAA GAAGACGGAG
    AAATGCACCA ACACCCACAG TCCCAAATGG AAACAGTCTC TCACTGTGAT CGTCACTCCT
    TTCAGCAAGC TAATTTTCCG GGTTTGGAGC CACCAGACAC TAAAAGCGGA CATTTTGCTT
    GGCGTGGCCA GTCTGGAGGT CAGCGAGACC TTAAAAGCCA ACAACATGAA GATCTGTGAG
    GTGGTGCAGA CGCTGCAGTT GAGCTCAGAC AGAGGGCGCT CGGAGCAGGT TGGAGATCTG
    TCTTTGTGTC TGGATGGACT TCAGGTGGAT GCAGAGACTT TCGCTTCAGA GGAACAAAAG
    CACAATGCTG TTGTTAATGG AGAATCCAGA CTGAACGGGG ATGCCATAAG AAGTCGAGAC
    TCTTCTCCAT CTAGTGACAT CACTGACGAT GCGCCTTTGC CCAATGGCCA TGCAGTGGGA
    AGCTCCGCCT CTGCATCTCC ATCCACTGGG GCTTTGAGAG CACCGCGTCC CCTTCGACCC
    CAGAGACCAC CGCCTGCTTC ACCACATAAA CATACTTCCT CCACAGCATC CTCTACTGGC
    TCGACTCCCA CTCACGGCAG CAGCGCTCCC AGTCCAGAGA TGTCTCCACG AGTGTGTGTG
    AATGGAGAAA CCGAGAGTCA AGGGTCAAGC TCGGGCTCAG GCTCAAATCA GGCACCCAGA
    GCCAGTCCAA CACCACCCAG AGCTCCAGCC ATCACCAACG GCCCTCTTCC TCCAGGCTGG
    GAGCAGAGAG CGGATCAGAA CGGACGCGTT TACTATGTTG ATCACATCGA GAAAAGAACC
    ACATGGGAAA GACCTGAACC TCTGCCACCA GGAACCACCA CATGGCAGAG ACCCACGCTA
    GAGTCGGTGC GCAACTATGA GGAATGGCAG AACCAGCGCA GTCAACTTCA GGGAGCCATG
    CAGCGCTTCA ACCAGAGGTT CATCTATGGC CTGCAGGAAC AGCTCGCGCC GACAGCTAAT
    AAAGAGTTTG ACCCTCTGGG ACCGCTGCCG TCAGGATGGG AGAAACGTAC CGACAGTAAT
    AGGAGGATGT ATTTTGTTCA CCATCCTACA AGATCCACAC AATGGGAAGA CCCTCGCACA
    CAAGGGTTGC TAAATGAGAA GCCTTTGCCG GAAGGTTGGG AAATGAGGTT TACAGTGGAT
    GGGATTCCCT ATTTTGTGGA TCACAACAGA AGAACTACAA CATACATAGA CCCCCGTACA
    GGAAAATCCT CGCTTGAGAA CGGCCCTCAG ATCACATACG TACGAGATTT TAAAGCCAAA
    GTGCACTATT TCCGCTTCTG GTGTCAGCAA TTATCAATGC CTCAACACAT CAAAATCCAT
    GTGAGTCGCA AAACGCTGTT TGAGGACTCA TTTCAGCAGA TTATGGGTTG CCATCCACAA
    GACCTGAGGC GAAGATTGTG GATCATTTTC CCAGGGGAGG AGGGGCTTGA CTATGGAGGC
    GTGGCCAGGG AATGGTTTTT CCTGCTCTCG CATGAGGTCC TGAACCCCAT GTACTGTCTG
    TTTGAATACG CCGGCAAAGA TAACTACTGC CTACAGATTA ACCCAGCATC CTCCATCAAC
    CCAGACCACC TCAAATACTT CAAGTTCATC GGCCGCTTCA TTGCTATGGC TTTGTTTCAT
    GGGAAGTTCA TCGACACCGG CTTCTCTTTG CCCTTTTATA AGCGCATCCT GAACAAACCT
    TTAGCTCTGA AGGACCTGGA GTCCATCGAC CCCGAGTTTT ACAACTCTCT CATCTGGATC
    AAGGATAATA ATATCGAGGA GTGTGGCTTG GAGATGTTTT TCTCTGTGGA TAAGGAGATT
    CTGGGAGAGG TCACAACTCA TGAACTCAAA CCTGATGGAG GAAACATTCA GGTGACGGAG
    GAAAATAAAG AGGAGTATAT CAGGTTGGTT GCTGAATGGA GGTTGTCCAG AGGAGTCGAG
    GAGCAAACTC AGGCCTTCCT CGAGGGCTTT AATGATGTCC TGCCGCAGCA GTACCTGCAA
    TACTTTGACG CCAAAGAACT TGAGGTTATG CTGTGTGGGA TGCAGGAGAT CGATCTGGGC
    GACTGGCAGA GGAACTCTAT CTACAGACAC TACGCTCGCA GCAGCAAACA GATCGTCTGG
    TTCTGGCAGT TTGTGAAGGA GATGGACAAT GAAAAGCGCA TGCGTCTGCT GCAGTTTGTG
    ACCGGCACAT GCAGACTTCC CGTTGGGGGA TTCGCTGATC TTATGGGCAG CAACGGACCG
    CAAAAGTTTT GCATTGAAAA AGTGGGAAAA GAAAACTGGC TGCCGAGGAG CCACACATGC
    TTTAACCGTC TAGACCTGCC GCCCTATAAA AGCTATGAAC AGTTAAAGGA GAAGCTGCTC
    TTCGCCATCG AGGAAACAGA AGGTTTCGGT CAGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301034.1
    CDS183..2681
    Translation

    Target ORF information:

    RefSeq Version XM_009302759.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio itchy E3 ubiquitin protein ligase a (itcha), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302759.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGGCTC AACTTCAGAT AACAGTTCTG TCTGCTAAGC TGCGGGAGAA TAAGAAGAAC 
    TGGTTTGGGC CCAGTCCTTA TGTGGAGGTG TTTGTGGACG GACAGTCGAA GAAGACGGAG
    AAATGCACCA ACACCCACAG TCCCAAATGG AAACAGTCTC TCACTGTGAT CGTCACTCCT
    TTCAGCAAGC TAATTTTCCG GGTTTGGAGC CACCAGACAC TAAAAGCGGA CATTTTGCTT
    GGCGTGGCCA GTCTGGAGGT CAGCGAGACC TTAAAAGCCA ACAACATGAA GATCTGTGAG
    GTGGTGCAGA CGCTGCAGTT GAGCTCAGAC AGAGGGCGCT CGGAGCAGGT TGGAGATCTG
    TCTTTGTGTC TGGATGGACT TCAGGTGGAT GCAGAGACTT TCGCTTCAGA GGAACAAAAG
    CACAATGCTG TTGTTAATGG AGAATCCAGA CTGAACGGGG ATGCCATAAG AAGTCGAGAC
    TCTTCTCCAT CTAGTGACAT CACTGACGAT GCGCCTTTGC CCAATGGCCA TGCAGTGGGA
    AGCTCCGCCT CTGCATCTCC ATCCACTGGG GCTTTGAGAG CACCGCGTCC CCTTCGACCC
    CAGAGACCAC CGCCTGCTTC ACCACATAAA CATACTTCCT CCACAGCATC CTCTACTGGC
    TCGACTCCCA CTCACGGCAG CAGCGCTCCC AGTCCAGAGA TGTCTCCACG AGTGTGTGTG
    AATGGAGAAA CCGAGAGTCA AGGGTCAAGC TCGGGCTCAG GCTCAAATCA GGCACCCAGA
    GCCAGTCCAA CACCACCCAG AGCTCCAGCC ATCACCAACG GCCCTCTTCC TCCAGGCTGG
    GAGCAGAGAG CGGATCAGAA CGGACGCGTT TACTATGTTG ATCACATCGA GAAAAGAACC
    ACATGGGAAA GACCTGAACC TCTGCCACCA GGAACCACCA CATGGCAGAG ACCCACGCTA
    GAGTCGGTGC GCAACTATGA GGAATGGCAG AACCAGCGCA GTCAACTTCA GGGAGCCATG
    CAGCGCTTCA ACCAGAGGTT CATCTATGGC CTGCAGGAAC AGCTCGCGCC GACAGCTAAT
    AAAGAGTTTG ACCCTCTGGG ACCGCTGCCG TCAGGATGGG AGAAACGTAC CGACAGTAAT
    AGGAGGATGT ATTTTGTTCA CCATCCTACA AGATCCACAC AATGGGAAGA CCCTCGCACA
    CAAGGGTTGC TAAATGAGAA GCCTTTGCCG GAAGGTTGGG AAATGAGGTT TACAGTGGAT
    GGGATTCCCT ATTTTGTGGA TCACAACAGA AGAACTACAA CATACATAGA CCCCCGTACA
    GGAAAATCCT CGCTTGAGAA CGGCCCTCAG ATCACATACG TACGAGATTT TAAAGCCAAA
    GTGCACTATT TCCGCTTCTG GTGTCAGCAA TTATCAATGC CTCAACACAT CAAAATCCAT
    GTGAGTCGCA AAACGCTGTT TGAGGACTCA TTTCAGCAGA TTATGGGTTG CCATCCACAA
    GACCTGAGGC GAAGATTGTG GATCATTTTC CCAGGGGAGG AGGGGCTTGA CTATGGAGGC
    GTGGCCAGGG AATGGTTTTT CCTGCTCTCG CATGAGGTCC TGAACCCCAT GTACTGTCTG
    TTTGAATACG CCGGCAAAGA TAACTACTGC CTACAGATTA ACCCAGCATC CTCCATCAAC
    CCAGACCACC TCAAATACTT CAAGTTCATC GGCCGCTTCA TTGCTATGGC TTTGTTTCAT
    GGGAAGTTCA TCGACACCGG CTTCTCTTTG CCCTTTTATA AGCGCATCCT GAACAAACCT
    TTAGCTCTGA AGGACCTGGA GTCCATCGAC CCCGAGTTTT ACAACTCTCT CATCTGGATC
    AAGGATAATA ATATCGAGGA GTGTGGCTTG GAGATGTTTT TCTCTGTGGA TAAGGAGATT
    CTGGGAGAGG TCACAACTCA TGAACTCAAA CCTGATGGAG GAAACATTCA GGTGACGGAG
    GAAAATAAAG AGGAGTATAT CAGGTTGGTT GCTGAATGGA GGTTGTCCAG AGGAGTCGAG
    GAGCAAACTC AGGCCTTCCT CGAGGGCTTT AATGATGTCC TGCCGCAGCA GTACCTGCAA
    TACTTTGACG CCAAAGAACT TGAGGTTATG CTGTGTGGGA TGCAGGAGAT CGATCTGGGC
    GACTGGCAGA GGAACTCTAT CTACAGACAC TACGCTCGCA GCAGCAAACA GATCGTCTGG
    TTCTGGCAGT TTGTGAAGGA GATGGACAAT GAAAAGCGCA TGCGTCTGCT GCAGTTTGTG
    ACCGGCACAT GCAGACTTCC CGTTGGGGGA TTCGCTGATC TTATGGGCAG CAACGGACCG
    CAAAAGTTTT GCATTGAAAA AGTGGGAAAA GAAAACTGGC TGCCGAGGAG CCACACATGC
    TTTAACCGTC TAGACCTGCC GCCCTATAAA AGCTATGAAC AGTTAAAGGA GAAGCTGCTC
    TTCGCCATCG AGGAAACAGA AGGTTTCGGT CAGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.