tsc1b cDNA ORF clone, Danio rerio(zebrafish)

The following tsc1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tsc1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa35747 NM_001282392.1
Latest version!
Danio rerio TSC complex subunit 1b (tsc1b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa35747 XM_009295278.2
Latest version!
Danio rerio tuberous sclerosis 1b (tsc1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa35747
    Clone ID Related Accession (Same CDS sequence) XM_009295278.2 , NM_001282392.1
    Accession Version NM_001282392.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-09
    Organism Danio rerio(zebrafish)
    Product hamartin
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX088716.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01019961.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCCAGGG AACAGCTGAA CGTGGGGGAT CTTCTTCCCC TGTTGGAGAC CTCTGACCTC 
    CGCCAGCTGA ACGAGATCAA GGGCCTTATT AATGAACACC TCAACACAGA TCGAGGGTCC
    GTGCTGCTGA ATGGTTTGGT GGATTATTTC CTGGAGACCA ACTCTGCCCA GACAGTGCAC
    ATCCTGTCTT CAGTGAGGGA GCCACATGAT AAGCACCTTT TTGATAAAAT GAATGAATGC
    ATGGCTAAAG CCCCCTGTCG CCTGCCCACC CTCACTCTCC TGGGGCATGT TGTCCGTAAG
    CAGCCATCTT GGATCCACAA GATCGCGCAC TATCCGCTGC TGCTATCTCT CCTCAAATGC
    CTCAAGACAG ACACAGATGT TGTGGTTCTG ATAACTGGAG TCCTGGTGCT CATCACACTT
    TTACCCATGA TTCCTCAAGC TGGCAAGCAA CATCTCTATG AGTTTTTTGA TATCTTTGGA
    CGTCTGGCGT CTTGGAACCT AAGAAACCCA GGACATGTAC CAGAGGTTTA TCTCATCCAT
    CTGCACGCAA GCGTTTATTC ACTTTTCCAC CGGCTGTATG GGATGTACCC CTGCAATTTT
    GTTTCATACC TTCGCTCCCA TTACAGTATG AAGGAGAACA TGGATACTTT TGAGGAGGTG
    GTCAAGCCCA TGCTTGAACA TGTCAGAATA CATCCTGAAT TAGTGACCGG AACCAAGGAC
    CAAGAGCTTG ACCCCAGCCG GTGGAAGAGA TTCGAAATCC ACGATATAGT TATCGAGTGT
    GCCAAAGTCT CTCTGGACCC AAAAGAGGCG TCCTGTGAAG AGGGCTATGC CAGCATGCCT
    GAGCATTTCT GTGCTCATCT ACAACAACGT CCGGCTGATT GCACTGCCAG TCCGTATGGT
    GACTTCCACA GTAGCTACGG AAGTTCTTCT TCGACTCATT TCTCCACCCC TCGTCAGCCT
    GTCGTCCCTC CCTTGGCCTT GTCCTCTATC TCAGGGACTC AGCCCCCCTA CCGCAGCCCC
    ACTGGAACCC GTCGGCAGAA CTCCAGCTGT GAATTTAATG CCACATATGG GGTTAAGGAT
    TCTCTCTGGA GCCCCTCCTC CCAGTGTGGG ATGGCCACTC CCCCTTCCTC CCGTGGGATG
    TCACCAAACC CGGAACTGTC CCAGAGTGCC TCTCACCTAC CGAGCCGCCT CTACAGCACA
    CCTGCAGGTG GAAAAGGCAC CCCGTCCTCC AGCACGCCTG CAGCTGCAAC ACCTCCTCCT
    AGTCTATCAG ATGAGTATCC CCCTGTATCG ACGCCACGCA GCACCGCCAG CCCACCATGC
    AAGGATGGTA GACCTTTAGA CCATATTAAA CCGGTCTTGG TCAGACAAGA GCAACTCAGA
    GAGTCTGAAA AAACTGGGCC TGAAACGATG AATCACAGAG ATGGCAGAGG TGAAAACATG
    CCCATGACCC TGACTGAGCT CTCTGTGTAC ATGAAGGAGC AAGAACAACT GAGGATCGAG
    AAGGAGAGGG AGGATGCTGC CATCACAGAG GAGCTCCTGA AGTTAACCGA GGAGAAGCGG
    GATCTAGCCG GCCTCCGTGG CTTCGACTCT CCATTCTTCA GCACTACAGA GACCCTGACT
    GGAGCACAAC AGAAACCCCT GCCCTCCTCT ATTCTTCACA ACACCATCTC CACCCCTGAC
    AAAACTGAAA TAACACCCAG TGACAAAGGA AGAGTCAGCA GAGGCCCATC CCAAGAGCAA
    CCCTGGTCCT TCCAGCCAGT GTTCATGCCC ATCGAACACC CGACGCACAG CCTCCTTCAT
    CGGAGCCCGT CAGCACCAGA TGACGAGGCC TTAAAATTTG GCTTGTGTTC CCCGAGCCCA
    TGCAAACCTC CACCCATGCC ATACGAGGCT CTGTTTGACA TGGCTCTGCC TCGGGCTGCC
    TCTCTTTTTG TGGGAAGGAA GACTTCAGAG ATTGTGCAGC GAGTGGCGAT GGAGAGGTTT
    CAGAGAGGGG ATTCTGGCAG GCAAGGGGAG GACGAGGGTG TGGAGGGGGT CGCCTCTGCT
    TCGCCTCTGG AGGTGCTGGA TCGCCTCGTG CAGCAGGGTG GAGACGCCCA TGACAAAGTG
    CTAAAGAGAC TGCCCCTGCC AAGCAAGTCA GCTGACTGGA CACACTTTGG AGGTTCAGCT
    CCTCTGGATG AGTTGCACAC ACTGCGCAGT CAGCTTCTGC TTCTACACAA CCAGCTCCTG
    TATGAGCGCT ATAAGCGAGA GCAGCACGCC GTTCGCAACC GACGTCTGCT TAGGCGAATC
    ATCAACGCCA CTGCACTCGA GGAGCAGAAT AATGCTATGA AGGACCAGCT GAACCTGCAA
    AGTGTGGACA TCTTGTCTTT GAGAGAGAGC CTTCAGGTGG AGCAGCAGAG ATACCGGCAG
    CTCTGGGACG ACCGAGAGAC TGTAGTTACA CGCCTGCACA GCCAGATACG ACAACTGCAG
    CAAGCCAGGG ATGACTACTA CACTAAAAAC CAGGAGTTAC AGAGCAAGCT TCAAGAATGC
    CAGAAGCGAA TAGGAGAGAT GGAGGCGGAG CTTCAGAAGG CCAATAATGA GGTGTGCCAC
    ACAGGACACC TGCTGAACCA GCTTACTGCC AAGTTGTCCT CTAGTGAAAG TATTCAGCAG
    CAGTTGAGTT TTCTGAATAA GCAGCTGTTG CTGTTGGGAG AAGCCCATAA ACTGTGTGTT
    CAGGAGCTTC ATCAGGCTGG ACCGGATACT AGCAAGGAGG TGCAGATGTT GCAGACGTCT
    TGGGGGAAGG AGACGGAGCG TCTGAAACAG AATCTGTTAC TTCAGAGCCA AAAGCTGGAC
    GCGGCTCAGC AGAGAGTGGC GGAGCTGGAG ACTCAGCTCT CTAAGAAGGA GCACCTGATC
    GCAGAACAAA AGAAGTTTTT GGAAGATGTC AAGGCTCAGG CTAAAGGGGA GCTGCAAGCT
    TCTGAGAGCA GGTATCAGGC ACAGAGGAGA GTGACTCTGG CTCTGCAGAC TGAGCTTCTG
    CAGCTCTATA GCAGGCTGGA AACAGAAGAT TCTTCTGCTG CTGATTCGGC CTTACAGGCA
    GAGGGTGTTA CTGAGCCTTG CATTACCACT GAGCCCAGTG TCATAGTAGT CGGCGGTGCC
    ATCAAAACAG CCACAAAAGA GGACGGAAAG CCACATCAGT CTCCACGTGG CTATGGCAGC
    ACTTTATCAC CAGGCCATCC CAAAGCCAGC TCTTCTCCGG ACAACTCGGT GAACGGCAAT
    CGAGACACTC CCCCGCCTGT CCTGGTGGAA CCACCCCCTC ACCGTCCCCA TTCCCCCCTG
    GGACCCCCTG GACCCCTGGT GTCGTCTGAC ACCCCACTCA CCGTAGGCTC CTACCCCAGC
    GCCAAGAGCT TTCTGGGCAT GCGGGCACGA GAGTTATTCC GCAACAAAAG CGAAAGCCAG
    TGTGACGAGG AAGAAACTCC GCCTCCTCGT CTAACTGGCC TATCACAGGG CCTGAAAACT
    GAGCTCTGCA CAGAGACGAC AGCAAAGCGT CCGTCCGTCT CCACCACCGC ACAAAGCGTC
    ACGCTTATCA CATCTGGTAA AGAGTCCCAA CCCGACGCAC AGAGAGCCAA AAAACAAGAC
    ACGTTAGGTT CAAGATGCCC AGAGCGACCC AGGCAGCAAC CGTTGAGAAT CATGGACTAT
    AACGAAAGTC GTCATGAGCA TAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269321.1
    CDS150..3836
    Translation

    Target ORF information:

    RefSeq Version NM_001282392.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio TSC complex subunit 1b (tsc1b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282392.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCCAGGG AACAGCTGAA CGTGGGGGAT CTTCTTCCCC TGTTGGAGAC CTCTGACCTC 
    CGCCAGCTGA ACGAGATCAA GGGCCTTATT AATGAACACC TCAACACAGA TCGAGGGTCC
    GTGCTGCTGA ATGGTTTGGT GGATTATTTC CTGGAGACCA ACTCTGCCCA GACAGTGCAC
    ATCCTGTCTT CAGTGAGGGA GCCACATGAT AAGCACCTTT TTGATAAAAT GAATGAATGC
    ATGGCTAAAG CCCCCTGTCG CCTGCCCACC CTCACTCTCC TGGGGCATGT TGTCCGTAAG
    CAGCCATCTT GGATCCACAA GATCGCGCAC TATCCGCTGC TGCTATCTCT CCTCAAATGC
    CTCAAGACAG ACACAGATGT TGTGGTTCTG ATAACTGGAG TCCTGGTGCT CATCACACTT
    TTACCCATGA TTCCTCAAGC TGGCAAGCAA CATCTCTATG AGTTTTTTGA TATCTTTGGA
    CGTCTGGCGT CTTGGAACCT AAGAAACCCA GGACATGTAC CAGAGGTTTA TCTCATCCAT
    CTGCACGCAA GCGTTTATTC ACTTTTCCAC CGGCTGTATG GGATGTACCC CTGCAATTTT
    GTTTCATACC TTCGCTCCCA TTACAGTATG AAGGAGAACA TGGATACTTT TGAGGAGGTG
    GTCAAGCCCA TGCTTGAACA TGTCAGAATA CATCCTGAAT TAGTGACCGG AACCAAGGAC
    CAAGAGCTTG ACCCCAGCCG GTGGAAGAGA TTCGAAATCC ACGATATAGT TATCGAGTGT
    GCCAAAGTCT CTCTGGACCC AAAAGAGGCG TCCTGTGAAG AGGGCTATGC CAGCATGCCT
    GAGCATTTCT GTGCTCATCT ACAACAACGT CCGGCTGATT GCACTGCCAG TCCGTATGGT
    GACTTCCACA GTAGCTACGG AAGTTCTTCT TCGACTCATT TCTCCACCCC TCGTCAGCCT
    GTCGTCCCTC CCTTGGCCTT GTCCTCTATC TCAGGGACTC AGCCCCCCTA CCGCAGCCCC
    ACTGGAACCC GTCGGCAGAA CTCCAGCTGT GAATTTAATG CCACATATGG GGTTAAGGAT
    TCTCTCTGGA GCCCCTCCTC CCAGTGTGGG ATGGCCACTC CCCCTTCCTC CCGTGGGATG
    TCACCAAACC CGGAACTGTC CCAGAGTGCC TCTCACCTAC CGAGCCGCCT CTACAGCACA
    CCTGCAGGTG GAAAAGGCAC CCCGTCCTCC AGCACGCCTG CAGCTGCAAC ACCTCCTCCT
    AGTCTATCAG ATGAGTATCC CCCTGTATCG ACGCCACGCA GCACCGCCAG CCCACCATGC
    AAGGATGGTA GACCTTTAGA CCATATTAAA CCGGTCTTGG TCAGACAAGA GCAACTCAGA
    GAGTCTGAAA AAACTGGGCC TGAAACGATG AATCACAGAG ATGGCAGAGG TGAAAACATG
    CCCATGACCC TGACTGAGCT CTCTGTGTAC ATGAAGGAGC AAGAACAACT GAGGATCGAG
    AAGGAGAGGG AGGATGCTGC CATCACAGAG GAGCTCCTGA AGTTAACCGA GGAGAAGCGG
    GATCTAGCCG GCCTCCGTGG CTTCGACTCT CCATTCTTCA GCACTACAGA GACCCTGACT
    GGAGCACAAC AGAAACCCCT GCCCTCCTCT ATTCTTCACA ACACCATCTC CACCCCTGAC
    AAAACTGAAA TAACACCCAG TGACAAAGGA AGAGTCAGCA GAGGCCCATC CCAAGAGCAA
    CCCTGGTCCT TCCAGCCAGT GTTCATGCCC ATCGAACACC CGACGCACAG CCTCCTTCAT
    CGGAGCCCGT CAGCACCAGA TGACGAGGCC TTAAAATTTG GCTTGTGTTC CCCGAGCCCA
    TGCAAACCTC CACCCATGCC ATACGAGGCT CTGTTTGACA TGGCTCTGCC TCGGGCTGCC
    TCTCTTTTTG TGGGAAGGAA GACTTCAGAG ATTGTGCAGC GAGTGGCGAT GGAGAGGTTT
    CAGAGAGGGG ATTCTGGCAG GCAAGGGGAG GACGAGGGTG TGGAGGGGGT CGCCTCTGCT
    TCGCCTCTGG AGGTGCTGGA TCGCCTCGTG CAGCAGGGTG GAGACGCCCA TGACAAAGTG
    CTAAAGAGAC TGCCCCTGCC AAGCAAGTCA GCTGACTGGA CACACTTTGG AGGTTCAGCT
    CCTCTGGATG AGTTGCACAC ACTGCGCAGT CAGCTTCTGC TTCTACACAA CCAGCTCCTG
    TATGAGCGCT ATAAGCGAGA GCAGCACGCC GTTCGCAACC GACGTCTGCT TAGGCGAATC
    ATCAACGCCA CTGCACTCGA GGAGCAGAAT AATGCTATGA AGGACCAGCT GAACCTGCAA
    AGTGTGGACA TCTTGTCTTT GAGAGAGAGC CTTCAGGTGG AGCAGCAGAG ATACCGGCAG
    CTCTGGGACG ACCGAGAGAC TGTAGTTACA CGCCTGCACA GCCAGATACG ACAACTGCAG
    CAAGCCAGGG ATGACTACTA CACTAAAAAC CAGGAGTTAC AGAGCAAGCT TCAAGAATGC
    CAGAAGCGAA TAGGAGAGAT GGAGGCGGAG CTTCAGAAGG CCAATAATGA GGTGTGCCAC
    ACAGGACACC TGCTGAACCA GCTTACTGCC AAGTTGTCCT CTAGTGAAAG TATTCAGCAG
    CAGTTGAGTT TTCTGAATAA GCAGCTGTTG CTGTTGGGAG AAGCCCATAA ACTGTGTGTT
    CAGGAGCTTC ATCAGGCTGG ACCGGATACT AGCAAGGAGG TGCAGATGTT GCAGACGTCT
    TGGGGGAAGG AGACGGAGCG TCTGAAACAG AATCTGTTAC TTCAGAGCCA AAAGCTGGAC
    GCGGCTCAGC AGAGAGTGGC GGAGCTGGAG ACTCAGCTCT CTAAGAAGGA GCACCTGATC
    GCAGAACAAA AGAAGTTTTT GGAAGATGTC AAGGCTCAGG CTAAAGGGGA GCTGCAAGCT
    TCTGAGAGCA GGTATCAGGC ACAGAGGAGA GTGACTCTGG CTCTGCAGAC TGAGCTTCTG
    CAGCTCTATA GCAGGCTGGA AACAGAAGAT TCTTCTGCTG CTGATTCGGC CTTACAGGCA
    GAGGGTGTTA CTGAGCCTTG CATTACCACT GAGCCCAGTG TCATAGTAGT CGGCGGTGCC
    ATCAAAACAG CCACAAAAGA GGACGGAAAG CCACATCAGT CTCCACGTGG CTATGGCAGC
    ACTTTATCAC CAGGCCATCC CAAAGCCAGC TCTTCTCCGG ACAACTCGGT GAACGGCAAT
    CGAGACACTC CCCCGCCTGT CCTGGTGGAA CCACCCCCTC ACCGTCCCCA TTCCCCCCTG
    GGACCCCCTG GACCCCTGGT GTCGTCTGAC ACCCCACTCA CCGTAGGCTC CTACCCCAGC
    GCCAAGAGCT TTCTGGGCAT GCGGGCACGA GAGTTATTCC GCAACAAAAG CGAAAGCCAG
    TGTGACGAGG AAGAAACTCC GCCTCCTCGT CTAACTGGCC TATCACAGGG CCTGAAAACT
    GAGCTCTGCA CAGAGACGAC AGCAAAGCGT CCGTCCGTCT CCACCACCGC ACAAAGCGTC
    ACGCTTATCA CATCTGGTAA AGAGTCCCAA CCCGACGCAC AGAGAGCCAA AAAACAAGAC
    ACGTTAGGTT CAAGATGCCC AGAGCGACCC AGGCAGCAAC CGTTGAGAAT CATGGACTAT
    AACGAAAGTC GTCATGAGCA TAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa35747
    Clone ID Related Accession (Same CDS sequence) XM_009295278.2 , NM_001282392.1
    Accession Version XM_009295278.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product hamartin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295278.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCCAGGG AACAGCTGAA CGTGGGGGAT CTTCTTCCCC TGTTGGAGAC CTCTGACCTC 
    CGCCAGCTGA ACGAGATCAA GGGCCTTATT AATGAACACC TCAACACAGA TCGAGGGTCC
    GTGCTGCTGA ATGGTTTGGT GGATTATTTC CTGGAGACCA ACTCTGCCCA GACAGTGCAC
    ATCCTGTCTT CAGTGAGGGA GCCACATGAT AAGCACCTTT TTGATAAAAT GAATGAATGC
    ATGGCTAAAG CCCCCTGTCG CCTGCCCACC CTCACTCTCC TGGGGCATGT TGTCCGTAAG
    CAGCCATCTT GGATCCACAA GATCGCGCAC TATCCGCTGC TGCTATCTCT CCTCAAATGC
    CTCAAGACAG ACACAGATGT TGTGGTTCTG ATAACTGGAG TCCTGGTGCT CATCACACTT
    TTACCCATGA TTCCTCAAGC TGGCAAGCAA CATCTCTATG AGTTTTTTGA TATCTTTGGA
    CGTCTGGCGT CTTGGAACCT AAGAAACCCA GGACATGTAC CAGAGGTTTA TCTCATCCAT
    CTGCACGCAA GCGTTTATTC ACTTTTCCAC CGGCTGTATG GGATGTACCC CTGCAATTTT
    GTTTCATACC TTCGCTCCCA TTACAGTATG AAGGAGAACA TGGATACTTT TGAGGAGGTG
    GTCAAGCCCA TGCTTGAACA TGTCAGAATA CATCCTGAAT TAGTGACCGG AACCAAGGAC
    CAAGAGCTTG ACCCCAGCCG GTGGAAGAGA TTCGAAATCC ACGATATAGT TATCGAGTGT
    GCCAAAGTCT CTCTGGACCC AAAAGAGGCG TCCTGTGAAG AGGGCTATGC CAGCATGCCT
    GAGCATTTCT GTGCTCATCT ACAACAACGT CCGGCTGATT GCACTGCCAG TCCGTATGGT
    GACTTCCACA GTAGCTACGG AAGTTCTTCT TCGACTCATT TCTCCACCCC TCGTCAGCCT
    GTCGTCCCTC CCTTGGCCTT GTCCTCTATC TCAGGGACTC AGCCCCCCTA CCGCAGCCCC
    ACTGGAACCC GTCGGCAGAA CTCCAGCTGT GAATTTAATG CCACATATGG GGTTAAGGAT
    TCTCTCTGGA GCCCCTCCTC CCAGTGTGGG ATGGCCACTC CCCCTTCCTC CCGTGGGATG
    TCACCAAACC CGGAACTGTC CCAGAGTGCC TCTCACCTAC CGAGCCGCCT CTACAGCACA
    CCTGCAGGTG GAAAAGGCAC CCCGTCCTCC AGCACGCCTG CAGCTGCAAC ACCTCCTCCT
    AGTCTATCAG ATGAGTATCC CCCTGTATCG ACGCCACGCA GCACCGCCAG CCCACCATGC
    AAGGATGGTA GACCTTTAGA CCATATTAAA CCGGTCTTGG TCAGACAAGA GCAACTCAGA
    GAGTCTGAAA AAACTGGGCC TGAAACGATG AATCACAGAG ATGGCAGAGG TGAAAACATG
    CCCATGACCC TGACTGAGCT CTCTGTGTAC ATGAAGGAGC AAGAACAACT GAGGATCGAG
    AAGGAGAGGG AGGATGCTGC CATCACAGAG GAGCTCCTGA AGTTAACCGA GGAGAAGCGG
    GATCTAGCCG GCCTCCGTGG CTTCGACTCT CCATTCTTCA GCACTACAGA GACCCTGACT
    GGAGCACAAC AGAAACCCCT GCCCTCCTCT ATTCTTCACA ACACCATCTC CACCCCTGAC
    AAAACTGAAA TAACACCCAG TGACAAAGGA AGAGTCAGCA GAGGCCCATC CCAAGAGCAA
    CCCTGGTCCT TCCAGCCAGT GTTCATGCCC ATCGAACACC CGACGCACAG CCTCCTTCAT
    CGGAGCCCGT CAGCACCAGA TGACGAGGCC TTAAAATTTG GCTTGTGTTC CCCGAGCCCA
    TGCAAACCTC CACCCATGCC ATACGAGGCT CTGTTTGACA TGGCTCTGCC TCGGGCTGCC
    TCTCTTTTTG TGGGAAGGAA GACTTCAGAG ATTGTGCAGC GAGTGGCGAT GGAGAGGTTT
    CAGAGAGGGG ATTCTGGCAG GCAAGGGGAG GACGAGGGTG TGGAGGGGGT CGCCTCTGCT
    TCGCCTCTGG AGGTGCTGGA TCGCCTCGTG CAGCAGGGTG GAGACGCCCA TGACAAAGTG
    CTAAAGAGAC TGCCCCTGCC AAGCAAGTCA GCTGACTGGA CACACTTTGG AGGTTCAGCT
    CCTCTGGATG AGTTGCACAC ACTGCGCAGT CAGCTTCTGC TTCTACACAA CCAGCTCCTG
    TATGAGCGCT ATAAGCGAGA GCAGCACGCC GTTCGCAACC GACGTCTGCT TAGGCGAATC
    ATCAACGCCA CTGCACTCGA GGAGCAGAAT AATGCTATGA AGGACCAGCT GAACCTGCAA
    AGTGTGGACA TCTTGTCTTT GAGAGAGAGC CTTCAGGTGG AGCAGCAGAG ATACCGGCAG
    CTCTGGGACG ACCGAGAGAC TGTAGTTACA CGCCTGCACA GCCAGATACG ACAACTGCAG
    CAAGCCAGGG ATGACTACTA CACTAAAAAC CAGGAGTTAC AGAGCAAGCT TCAAGAATGC
    CAGAAGCGAA TAGGAGAGAT GGAGGCGGAG CTTCAGAAGG CCAATAATGA GGTGTGCCAC
    ACAGGACACC TGCTGAACCA GCTTACTGCC AAGTTGTCCT CTAGTGAAAG TATTCAGCAG
    CAGTTGAGTT TTCTGAATAA GCAGCTGTTG CTGTTGGGAG AAGCCCATAA ACTGTGTGTT
    CAGGAGCTTC ATCAGGCTGG ACCGGATACT AGCAAGGAGG TGCAGATGTT GCAGACGTCT
    TGGGGGAAGG AGACGGAGCG TCTGAAACAG AATCTGTTAC TTCAGAGCCA AAAGCTGGAC
    GCGGCTCAGC AGAGAGTGGC GGAGCTGGAG ACTCAGCTCT CTAAGAAGGA GCACCTGATC
    GCAGAACAAA AGAAGTTTTT GGAAGATGTC AAGGCTCAGG CTAAAGGGGA GCTGCAAGCT
    TCTGAGAGCA GGTATCAGGC ACAGAGGAGA GTGACTCTGG CTCTGCAGAC TGAGCTTCTG
    CAGCTCTATA GCAGGCTGGA AACAGAAGAT TCTTCTGCTG CTGATTCGGC CTTACAGGCA
    GAGGGTGTTA CTGAGCCTTG CATTACCACT GAGCCCAGTG TCATAGTAGT CGGCGGTGCC
    ATCAAAACAG CCACAAAAGA GGACGGAAAG CCACATCAGT CTCCACGTGG CTATGGCAGC
    ACTTTATCAC CAGGCCATCC CAAAGCCAGC TCTTCTCCGG ACAACTCGGT GAACGGCAAT
    CGAGACACTC CCCCGCCTGT CCTGGTGGAA CCACCCCCTC ACCGTCCCCA TTCCCCCCTG
    GGACCCCCTG GACCCCTGGT GTCGTCTGAC ACCCCACTCA CCGTAGGCTC CTACCCCAGC
    GCCAAGAGCT TTCTGGGCAT GCGGGCACGA GAGTTATTCC GCAACAAAAG CGAAAGCCAG
    TGTGACGAGG AAGAAACTCC GCCTCCTCGT CTAACTGGCC TATCACAGGG CCTGAAAACT
    GAGCTCTGCA CAGAGACGAC AGCAAAGCGT CCGTCCGTCT CCACCACCGC ACAAAGCGTC
    ACGCTTATCA CATCTGGTAA AGAGTCCCAA CCCGACGCAC AGAGAGCCAA AAAACAAGAC
    ACGTTAGGTT CAAGATGCCC AGAGCGACCC AGGCAGCAAC CGTTGAGAAT CATGGACTAT
    AACGAAAGTC GTCATGAGCA TAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293553.1
    CDS282..3968
    Translation

    Target ORF information:

    RefSeq Version XM_009295278.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tuberous sclerosis 1b (tsc1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295278.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCCAGGG AACAGCTGAA CGTGGGGGAT CTTCTTCCCC TGTTGGAGAC CTCTGACCTC 
    CGCCAGCTGA ACGAGATCAA GGGCCTTATT AATGAACACC TCAACACAGA TCGAGGGTCC
    GTGCTGCTGA ATGGTTTGGT GGATTATTTC CTGGAGACCA ACTCTGCCCA GACAGTGCAC
    ATCCTGTCTT CAGTGAGGGA GCCACATGAT AAGCACCTTT TTGATAAAAT GAATGAATGC
    ATGGCTAAAG CCCCCTGTCG CCTGCCCACC CTCACTCTCC TGGGGCATGT TGTCCGTAAG
    CAGCCATCTT GGATCCACAA GATCGCGCAC TATCCGCTGC TGCTATCTCT CCTCAAATGC
    CTCAAGACAG ACACAGATGT TGTGGTTCTG ATAACTGGAG TCCTGGTGCT CATCACACTT
    TTACCCATGA TTCCTCAAGC TGGCAAGCAA CATCTCTATG AGTTTTTTGA TATCTTTGGA
    CGTCTGGCGT CTTGGAACCT AAGAAACCCA GGACATGTAC CAGAGGTTTA TCTCATCCAT
    CTGCACGCAA GCGTTTATTC ACTTTTCCAC CGGCTGTATG GGATGTACCC CTGCAATTTT
    GTTTCATACC TTCGCTCCCA TTACAGTATG AAGGAGAACA TGGATACTTT TGAGGAGGTG
    GTCAAGCCCA TGCTTGAACA TGTCAGAATA CATCCTGAAT TAGTGACCGG AACCAAGGAC
    CAAGAGCTTG ACCCCAGCCG GTGGAAGAGA TTCGAAATCC ACGATATAGT TATCGAGTGT
    GCCAAAGTCT CTCTGGACCC AAAAGAGGCG TCCTGTGAAG AGGGCTATGC CAGCATGCCT
    GAGCATTTCT GTGCTCATCT ACAACAACGT CCGGCTGATT GCACTGCCAG TCCGTATGGT
    GACTTCCACA GTAGCTACGG AAGTTCTTCT TCGACTCATT TCTCCACCCC TCGTCAGCCT
    GTCGTCCCTC CCTTGGCCTT GTCCTCTATC TCAGGGACTC AGCCCCCCTA CCGCAGCCCC
    ACTGGAACCC GTCGGCAGAA CTCCAGCTGT GAATTTAATG CCACATATGG GGTTAAGGAT
    TCTCTCTGGA GCCCCTCCTC CCAGTGTGGG ATGGCCACTC CCCCTTCCTC CCGTGGGATG
    TCACCAAACC CGGAACTGTC CCAGAGTGCC TCTCACCTAC CGAGCCGCCT CTACAGCACA
    CCTGCAGGTG GAAAAGGCAC CCCGTCCTCC AGCACGCCTG CAGCTGCAAC ACCTCCTCCT
    AGTCTATCAG ATGAGTATCC CCCTGTATCG ACGCCACGCA GCACCGCCAG CCCACCATGC
    AAGGATGGTA GACCTTTAGA CCATATTAAA CCGGTCTTGG TCAGACAAGA GCAACTCAGA
    GAGTCTGAAA AAACTGGGCC TGAAACGATG AATCACAGAG ATGGCAGAGG TGAAAACATG
    CCCATGACCC TGACTGAGCT CTCTGTGTAC ATGAAGGAGC AAGAACAACT GAGGATCGAG
    AAGGAGAGGG AGGATGCTGC CATCACAGAG GAGCTCCTGA AGTTAACCGA GGAGAAGCGG
    GATCTAGCCG GCCTCCGTGG CTTCGACTCT CCATTCTTCA GCACTACAGA GACCCTGACT
    GGAGCACAAC AGAAACCCCT GCCCTCCTCT ATTCTTCACA ACACCATCTC CACCCCTGAC
    AAAACTGAAA TAACACCCAG TGACAAAGGA AGAGTCAGCA GAGGCCCATC CCAAGAGCAA
    CCCTGGTCCT TCCAGCCAGT GTTCATGCCC ATCGAACACC CGACGCACAG CCTCCTTCAT
    CGGAGCCCGT CAGCACCAGA TGACGAGGCC TTAAAATTTG GCTTGTGTTC CCCGAGCCCA
    TGCAAACCTC CACCCATGCC ATACGAGGCT CTGTTTGACA TGGCTCTGCC TCGGGCTGCC
    TCTCTTTTTG TGGGAAGGAA GACTTCAGAG ATTGTGCAGC GAGTGGCGAT GGAGAGGTTT
    CAGAGAGGGG ATTCTGGCAG GCAAGGGGAG GACGAGGGTG TGGAGGGGGT CGCCTCTGCT
    TCGCCTCTGG AGGTGCTGGA TCGCCTCGTG CAGCAGGGTG GAGACGCCCA TGACAAAGTG
    CTAAAGAGAC TGCCCCTGCC AAGCAAGTCA GCTGACTGGA CACACTTTGG AGGTTCAGCT
    CCTCTGGATG AGTTGCACAC ACTGCGCAGT CAGCTTCTGC TTCTACACAA CCAGCTCCTG
    TATGAGCGCT ATAAGCGAGA GCAGCACGCC GTTCGCAACC GACGTCTGCT TAGGCGAATC
    ATCAACGCCA CTGCACTCGA GGAGCAGAAT AATGCTATGA AGGACCAGCT GAACCTGCAA
    AGTGTGGACA TCTTGTCTTT GAGAGAGAGC CTTCAGGTGG AGCAGCAGAG ATACCGGCAG
    CTCTGGGACG ACCGAGAGAC TGTAGTTACA CGCCTGCACA GCCAGATACG ACAACTGCAG
    CAAGCCAGGG ATGACTACTA CACTAAAAAC CAGGAGTTAC AGAGCAAGCT TCAAGAATGC
    CAGAAGCGAA TAGGAGAGAT GGAGGCGGAG CTTCAGAAGG CCAATAATGA GGTGTGCCAC
    ACAGGACACC TGCTGAACCA GCTTACTGCC AAGTTGTCCT CTAGTGAAAG TATTCAGCAG
    CAGTTGAGTT TTCTGAATAA GCAGCTGTTG CTGTTGGGAG AAGCCCATAA ACTGTGTGTT
    CAGGAGCTTC ATCAGGCTGG ACCGGATACT AGCAAGGAGG TGCAGATGTT GCAGACGTCT
    TGGGGGAAGG AGACGGAGCG TCTGAAACAG AATCTGTTAC TTCAGAGCCA AAAGCTGGAC
    GCGGCTCAGC AGAGAGTGGC GGAGCTGGAG ACTCAGCTCT CTAAGAAGGA GCACCTGATC
    GCAGAACAAA AGAAGTTTTT GGAAGATGTC AAGGCTCAGG CTAAAGGGGA GCTGCAAGCT
    TCTGAGAGCA GGTATCAGGC ACAGAGGAGA GTGACTCTGG CTCTGCAGAC TGAGCTTCTG
    CAGCTCTATA GCAGGCTGGA AACAGAAGAT TCTTCTGCTG CTGATTCGGC CTTACAGGCA
    GAGGGTGTTA CTGAGCCTTG CATTACCACT GAGCCCAGTG TCATAGTAGT CGGCGGTGCC
    ATCAAAACAG CCACAAAAGA GGACGGAAAG CCACATCAGT CTCCACGTGG CTATGGCAGC
    ACTTTATCAC CAGGCCATCC CAAAGCCAGC TCTTCTCCGG ACAACTCGGT GAACGGCAAT
    CGAGACACTC CCCCGCCTGT CCTGGTGGAA CCACCCCCTC ACCGTCCCCA TTCCCCCCTG
    GGACCCCCTG GACCCCTGGT GTCGTCTGAC ACCCCACTCA CCGTAGGCTC CTACCCCAGC
    GCCAAGAGCT TTCTGGGCAT GCGGGCACGA GAGTTATTCC GCAACAAAAG CGAAAGCCAG
    TGTGACGAGG AAGAAACTCC GCCTCCTCGT CTAACTGGCC TATCACAGGG CCTGAAAACT
    GAGCTCTGCA CAGAGACGAC AGCAAAGCGT CCGTCCGTCT CCACCACCGC ACAAAGCGTC
    ACGCTTATCA CATCTGGTAA AGAGTCCCAA CCCGACGCAC AGAGAGCCAA AAAACAAGAC
    ACGTTAGGTT CAAGATGCCC AGAGCGACCC AGGCAGCAAC CGTTGAGAAT CATGGACTAT
    AACGAAAGTC GTCATGAGCA TAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.