fgd5a cDNA ORF clone, Danio rerio(zebrafish)

The following fgd5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fgd5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa16993 XM_001919859.6
Latest version!
Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa16993 XM_021479795.1
Latest version!
Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa16994 XM_021479796.1
Latest version!
Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa16994 XM_005155840.4
Latest version!
Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa16994 XM_005155840.3 Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa16993 XM_001919859.5 Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa16993
    Clone ID Related Accession (Same CDS sequence) XM_001919859.5 , XM_021479795.1 , XM_001919859.6
    Accession Version XM_001919859.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product FYVE, RhoGEF and PH domain-containing protein 5 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001919859.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGTGCTCT ACACCTTTAA AGCACGAGAG GATAAAGTGG CATCTGAAAG TCTTTCACTG
    CAGGGTTTTA CTGTGAAGCT GTCGGATCGA TCAGAGGGTG AAGACACAGA CAACGTCTTC
    CAGCTCTACC ATAAAAAAAC ACTGTATTAC ACCTTCAGAG CGGACGACCA GCCCACTGCA
    CGCAGGTGGG TAAACGCAAT GGAAGAGGCC ACAGTGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001919894.2
    CDS797..5218
    Translation

    Target ORF information:

    RefSeq Version XM_001919859.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001919859.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGTGCTCT ACACCTTTAA AGCACGAGAG GATAAAGTGG CATCTGAAAG TCTTTCACTG
    CAGGGTTTTA CTGTGAAGCT GTCGGATCGA TCAGAGGGTG AAGACACAGA CAACGTCTTC
    CAGCTCTACC ATAAAAAAAC ACTGTATTAC ACCTTCAGAG CGGACGACCA GCCCACTGCA
    CGCAGGTGGG TAAACGCAAT GGAAGAGGCC ACAGTGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16993
    Clone ID Related Accession (Same CDS sequence) XM_001919859.5 , XM_021479795.1 , XM_001919859.6
    Accession Version XM_021479795.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FYVE, RhoGEF and PH domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGTGCTCT ACACCTTTAA AGCACGAGAG GATAAAGTGG CATCTGAAAG TCTTTCACTG
    CAGGGTTTTA CTGTGAAGCT GTCGGATCGA TCAGAGGGTG AAGACACAGA CAACGTCTTC
    CAGCTCTACC ATAAAAAAAC ACTGTATTAC ACCTTCAGAG CGGACGACCA GCCCACTGCA
    CGCAGGTGGG TAAACGCAAT GGAAGAGGCC ACAGTGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335470.1
    CDS823..5244
    Translation

    Target ORF information:

    RefSeq Version XM_021479795.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479795.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGTGCTCT ACACCTTTAA AGCACGAGAG GATAAAGTGG CATCTGAAAG TCTTTCACTG
    CAGGGTTTTA CTGTGAAGCT GTCGGATCGA TCAGAGGGTG AAGACACAGA CAACGTCTTC
    CAGCTCTACC ATAAAAAAAC ACTGTATTAC ACCTTCAGAG CGGACGACCA GCCCACTGCA
    CGCAGGTGGG TAAACGCAAT GGAAGAGGCC ACAGTGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16993
    Clone ID Related Accession (Same CDS sequence) XM_001919859.5 , XM_021479795.1 , XM_001919859.6
    Accession Version XM_001919859.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FYVE, RhoGEF and PH domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394814.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001919859.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGTGCTCT ACACCTTTAA AGCACGAGAG GATAAAGTGG CATCTGAAAG TCTTTCACTG
    CAGGGTTTTA CTGTGAAGCT GTCGGATCGA TCAGAGGGTG AAGACACAGA CAACGTCTTC
    CAGCTCTACC ATAAAAAAAC ACTGTATTAC ACCTTCAGAG CGGACGACCA GCCCACTGCA
    CGCAGGTGGG TAAACGCAAT GGAAGAGGCC ACAGTGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001919894.2
    CDS823..5244
    Translation

    Target ORF information:

    RefSeq Version XM_001919859.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001919859.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGTGCTCT ACACCTTTAA AGCACGAGAG GATAAAGTGG CATCTGAAAG TCTTTCACTG
    CAGGGTTTTA CTGTGAAGCT GTCGGATCGA TCAGAGGGTG AAGACACAGA CAACGTCTTC
    CAGCTCTACC ATAAAAAAAC ACTGTATTAC ACCTTCAGAG CGGACGACCA GCCCACTGCA
    CGCAGGTGGG TAAACGCAAT GGAAGAGGCC ACAGTGTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16994
    Clone ID Related Accession (Same CDS sequence) XM_005155840.4 , XM_005155840.3 , XM_021479796.1
    Accession Version XM_005155840.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FYVE, RhoGEF and PH domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394814.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155840.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGATAAAG TGGCATCTGA AAGTCTTTCA CTGCAGGGTT TTACTGTGAA GCTGTCGGAT
    CGATCAGAGG GTGAAGACAC AGACAACGTC TTCCAGCTCT ACCATAAAAA AACACTGTAT
    TACACCTTCA GAGCGGACGA CCAGCCCACT GCACGCAGGT GGGTAAACGC AATGGAAGAG
    GCCACAGTGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005155897.1
    CDS823..5217
    Translation

    Target ORF information:

    RefSeq Version XM_005155840.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005155840.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGATAAAG TGGCATCTGA AAGTCTTTCA CTGCAGGGTT TTACTGTGAA GCTGTCGGAT
    CGATCAGAGG GTGAAGACAC AGACAACGTC TTCCAGCTCT ACCATAAAAA AACACTGTAT
    TACACCTTCA GAGCGGACGA CCAGCCCACT GCACGCAGGT GGGTAAACGC AATGGAAGAG
    GCCACAGTGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16994
    Clone ID Related Accession (Same CDS sequence) XM_005155840.4 , XM_005155840.3 , XM_021479796.1
    Accession Version XM_005155840.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product FYVE, RhoGEF and PH domain-containing protein 5 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155840.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGATAAAG TGGCATCTGA AAGTCTTTCA CTGCAGGGTT TTACTGTGAA GCTGTCGGAT
    CGATCAGAGG GTGAAGACAC AGACAACGTC TTCCAGCTCT ACCATAAAAA AACACTGTAT
    TACACCTTCA GAGCGGACGA CCAGCCCACT GCACGCAGGT GGGTAAACGC AATGGAAGAG
    GCCACAGTGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005155897.1
    CDS797..5191
    Translation

    Target ORF information:

    RefSeq Version XM_005155840.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005155840.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGATAAAG TGGCATCTGA AAGTCTTTCA CTGCAGGGTT TTACTGTGAA GCTGTCGGAT
    CGATCAGAGG GTGAAGACAC AGACAACGTC TTCCAGCTCT ACCATAAAAA AACACTGTAT
    TACACCTTCA GAGCGGACGA CCAGCCCACT GCACGCAGGT GGGTAAACGC AATGGAAGAG
    GCCACAGTGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16994
    Clone ID Related Accession (Same CDS sequence) XM_005155840.4 , XM_005155840.3 , XM_021479796.1
    Accession Version XM_021479796.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FYVE, RhoGEF and PH domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGATAAAG TGGCATCTGA AAGTCTTTCA CTGCAGGGTT TTACTGTGAA GCTGTCGGAT
    CGATCAGAGG GTGAAGACAC AGACAACGTC TTCCAGCTCT ACCATAAAAA AACACTGTAT
    TACACCTTCA GAGCGGACGA CCAGCCCACT GCACGCAGGT GGGTAAACGC AATGGAAGAG
    GCCACAGTGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335471.1
    CDS823..5217
    Translation

    Target ORF information:

    RefSeq Version XM_021479796.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio FYVE, RhoGEF and PH domain containing 5a (fgd5a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479796.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTCAAGGC CTTGTCCTTC CACCCGAGGT CCTAAACCTC CTGTGGCACC CAAACCAAGG 
    CTGGCTTCAG AGGTGAGCCA TAGCATTGTG GGCAATGGGG ACATAACATC CTCTGACAGA
    CTGGAATTGA AGCAAGATAA TAAGTCAGAT GTTGCACAGG AGGAATCAGA TAAATCCAGA
    GAAAACTGTA CTGACGATCA AGCAGAGAAT GACTTATATG ACAAAGAGTT CGCTGTAAAT
    TATGACACTA CTTGCTATGA TGAAATAGAG GACAAATTGC AAATGGCAGT GGATGAAACA
    ATGGGAGAGG ATGAAGATAA AAATGTCACT GAAATACAGC ATGCAACAAC TTTGAGTTTA
    TCGGAAGCTG TTGATGAATG TCTTGATTCA GCAGATGAGT TCCACAGCAA AAGCGCTGAT
    GATATCAAGC CAGCTGAGAG CAGAGAAGTT ATTTATAATG TACTTGAAGA CACCAGTGCA
    TGTTCTGAGA ATGATACATC GACTAAAGCA TGTGAATCTC AAAGTCCTTA TGAGGAGATT
    GAGGAACCAC CATTAAACAG TTTTCAGTGC GCCAATGGTC AGTACGAAGA GGACATTGGA
    GCAGGTGCAA ACTGTGTCCA TCATGAAGCA GAAGAAGATG GGACTGGACT TTCTTCAAAG
    GCTACAAACT GCAGTCAACC CTCACTATGC GAGGAATACC CTTATGATAT CATTGGCACA
    TTGGATGACA ACAATACTTG GCAGGCTGAA CGGGCTACCA AAGACCCATC TGGACGCTTC
    AGGTTTGCAG AGGAGACAGA AGATGCATTT GAGCCCTACT CTGTCATTGA GGTGGTGCCA
    AGTGATTTGA TGTGCACTGC TGATCCAGAA ACAAGATGCA CTGATGAAGA GTCGAGCAAT
    GTAAAACCAA TGGACTCTGA GAACACAACA GAGGGAACAT CAAACCAAGA GCCTTACTAT
    GTGTCATCGG AAGATGTGGA AGATTTAGAG GAGGAACAGG CAAAATTAGA AAACGGTGTC
    TCTCTACCTG CTTCTGAGGT TCAGGAAATG GACAAAAATG AAGAGGTAGA TGATTATGCT
    GATATTGAAG ACCATGAAGG TGATGATCAC CAGGTGGAGT GTGCTTCGTC AGAGGATTAC
    GTCGAGATAG GAGATGAAGA TGAACCATTA AACATGGAGA GGAAAACCAA GGGCAAGAGT
    GTAAGGGAAC GGTCAGCTAG ACGACGACAT GATCTTTTGA GTCAGAGGAA CAGCTGCCAG
    CCACGTCTCA GACTATGCAA CATCACTGTT CCTGCTGACC TGGATTTAGG CAGAACTCCA
    GAACTTACAA ACCGTGTTGT TTTTGCCCAC ACAACAGAAG CCTTTGAAGA GGACATCGAA
    GAGCTGGACT GCCATATTGT GCCTTTCTTT GAGGACTCGG ACACAGAAAG TGACGAACAC
    ATATACGAGG AGGCTGGCTT CGACTCAGAG GGTGAGAACT TTATCTCACT GGACAGGAAG
    AGCATTGTGA CAAGATCTCG CTCGCTTTCT GGGAAAGTAC CTGGATATGT TCCTGAGACT
    GTGCCTGAGG AGACGGGCAC GGAGTACCAG TCTCACGACT ACTACACTGT GGCCTTAGAC
    CAGAATGGAG ATCCACATCC AAAGCCTTCA GATGAAGCGG AAGCCAATAG AATTATTCCT
    TCATTGAAAC CAAGGCGTTT TCTTCTTTCC CCTCGGTCAC TCTCAGTTGA GGGCAGAGAT
    TTGCAGCTCT CAGGGCACTT TGAGGGTGAG AAATCTCTTA GAGAGGAATA CAGACTGCAA
    AGAAAAGATG ACACACTTTC TTTGCCCTGT GTCATTACTT CCTCTGGAAG CTTTTCTCAA
    AGAAGTCACC AGTCCTCCAG TGGCGTGTCG ACACCTACAT CTCTAGTCGA TATCCCGCCT
    CCCTTTGAGT TAGCTTACAT AACTAAGCGG CCTGTTACCA AAAGCTCCCC ATCACTTCTA
    ATCCAACATG ATTCTCCTGA CAACCCCAAA AAGAAGAAGA CTTCATTTAA ACGCTTTTTG
    GCTTTAAAAT TTAGGAAAAA ATCAGAAAGC AAATCCCGGG GTGATGGCAG TGTCCGCTCC
    TCCAGGTCAT CGTCTGAGTC TAGCCATCAT GGGCCCATTC GAGTGTTGGA GCTTGATCGA
    AGGAGCACAA GTGGCTCCCC AAAGCTTCAG TCCCATGCAG GAAAACCTCA GCGTAACTCA
    GAAATACCCA CCACGTTTCT GCTTTACAAA GAGAGACAAA GTAGGCAGGG TGCACTCAAG
    AGTTATAACA GCAGGGGTAT ATCCAGAGTC GAGTCCTTTG AGGACCGCAC TCGACCCCCC
    TTCATGCCCT TACCACTCAC TAAGCCCCGC TCTATCTCCT TTCCAAGCGC AGATACGTCT
    GACTATGAAA ACATTCCTGC AATGAGTTCT GACTATGAGA ATATTCAGAT TCCACATGGG
    CGGCCTACAC GTGCAGTCAC TATTACAGAG TTCTTTGATG ACCACAATAG GACAGCAGCA
    CCTGCCAATG AGAACGATGG CTATGTGGAT ATGAATAGCT TTGCAGGAAT TGAAAACAAG
    TCCACCACTC GAGAGCAAGA TACTGAAAGT GCCTACACTG AGCCATTTCC TGTTTGTCCT
    GTGTCTGCAA CTGTGTCCAA TGAGGAAGAT CATGGACGCA CATCTGAGGA AGAGGATGGT
    TGTGGAGACC ACTGTCATGA TCGACAGATT GATGGCCGAT CAAGAGCCTA CTATGTTGCC
    AAAGACCTGG TGGACTCAGA AAGAGAACAC GTGAAGGCTC TGAAGGTTCT TCAAGAGGAT
    TTCAGGGCAG CAGTGGAGTC AGGAGGAGAT GATAACGGAC CTCTGATAGA AAATGGGAAG
    CTGGATGAGA TCTTCAGCAC TCTTCCTCAG GCATACAGCT TACACAGCGA GATCCTCAAC
    GAGCTGGAGA CCCGTATTAA ACAGTGGGAG GACAGTCCGA CAGTGGTTGA TGTGATATTA
    ACGCGCCGAG CTGAGTTTTC TTCTTTTGCG ACCTACATCT CCAACTATGA TCGCAGCATG
    TCAGTGCTGG ACGAGAGCTG TAGAAAGAGT CAAGCTTTCG AGGATGTGGT GAAACAGTTT
    GAGACACAGA ATGCAGAAGG AGTTCTTGTT CCTCTGAAAC ATCAGCTGCT CCGGGTCATT
    GTGCGTATTC TTCAATACCG CATGCTGCTT ACAGACTACT TGAACAACCT CTCCCCTGAT
    TCGAAAGAGT ATGAAGACAC ACAAGCTGCG CTGGTAATCG TGTCAGAGGT CGCAGACCAG
    GTTAATGACA ATCTAAAACA CGGGGAGAAC CTGCTTCGTT TGGTCCACAT CGACTACAGT
    GTAAGAGGAA AAAGAGACCT CCTTCAACCT GGAAGGGTAT TTGTTAAGGA GGGAACCCTG
    ATGAAGGTCT CTCGTAAAAG CAGGCAGCCC AGACATCTCT TCCTGATGAA TGATGTGATG
    CTGTACACCT ACCCTCAGCA GGACGGCAAA TACAGACTGA AGAACACACT CACTTTAAGT
    GGAATGAAAG TCAGTAAACC CATCATAGAC AATGTGCTGA ACACACTCAG AATAGAAGTC
    AGCGACGTCA CCATCACACT CTCAGCCAGC TCTTTAGGAG AAAGAGAAGA CTGGTTTCAC
    ACACTGAGTC GGGCTATCGC TGATCACGCC GCAGGCCTCA ATACCTTCAG CAGCTCCAGC
    GAGGCCAGAG AGAAGCTGTG GATGTCATTG GGAGAAGCTG CTCCTGTTCT GGTTCCTGTG
    TCTCATGTGA TGATGTGCAT GAACTGCACG TCTGACTTCA GTCTAACACT GCGACGGCAT
    CACTGTAATG CATGCGGAAA GGTTGTGTGT CGCTCCTGCT CAAGGAACAG GTATCCTCTA
    AAGTACCTGA AGGACAGGAT GGCTAAAGTC TGTGACCACT GCTATGCTGA GCTCAGAAAG
    AGAGGTGGTG ATGTTCCTGG ATCATGTGGG AGTGTGAGTC CACGCACACA CAGGGCCAGC
    AGACCTCTCT CAGCTGTGTT TCAGAGCCTG CAGCCACCCA GCCTTTGGAG GAACAGGAAG
    AGCAGCTCGC CTCTCACACA GGTGTCTGTG GGTGCAGAGG GGTCCACTAT GAGTGGCAGT
    TTGCAAAGAC GCAAAAAAAG CAAAAGGAGG TGGAAGAGGC TGTGGTTCCT GCTCAAAGAC
    AAGGATAAAG TGGCATCTGA AAGTCTTTCA CTGCAGGGTT TTACTGTGAA GCTGTCGGAT
    CGATCAGAGG GTGAAGACAC AGACAACGTC TTCCAGCTCT ACCATAAAAA AACACTGTAT
    TACACCTTCA GAGCGGACGA CCAGCCCACT GCACGCAGGT GGGTAAACGC AATGGAAGAG
    GCCACAGTGT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.