mcf2la cDNA ORF clone, Danio rerio(zebrafish)

The following mcf2la gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mcf2la cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa147440 XM_021477106.1
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa147441 XM_021477110.1
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa147442 XM_021477116.1
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa147443 XM_021477126.1
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa147444 XM_021477133.1
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa147445 XM_021477145.1
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa53916 XM_017356467.1
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa53917 XM_017356469.1
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa53918 XM_017356470.1
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa53919 XM_017356474.1
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa06135 XM_005171007.4
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa06137 XM_005171009.4
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa06138 XM_001922148.7
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa48644 XM_009293977.2
Latest version!
Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa06138 XM_001922148.6 Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa06135 XM_005171007.3 Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa06137 XM_005171009.3 Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa147440
    Clone ID Related Accession (Same CDS sequence) XM_021477106.1
    Accession Version XM_021477106.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGAGACT GTCGTCAGTC CCAACACTGA GAGAGCTGCA
    GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC TTCAGTAACC TCAAGAGTCA AAAAGGATCA
    TCCCCCCCTA GTCCCGACCA CAAAACTAAA AGACAGAGTG ATCCTACACC GTTTGGCTTC
    AGAGACGCAG GTCCTCACAT CACTCTCACC CGAGTGAGAT GGCTGAGCAC TTCTAGTCTG
    TTGCAGACTA AACGCAGAGG CTTCACCAAG GCGTCGCTGT CTATGGATGC GTCTGAAGAA
    AACGACGGAT ACTCGAGCGC CGAGGACCCC ATGAACTCTG ACCCTGAGGA TGAGGGAGGG
    AAGAAATTGT CTCCAGGCAA GTACGTAGTG GTTGCTGACC ATGACAAAAG TGGACCACAG
    GAGCTGACTG TTAAGAGCGG AGACACTGTG CAACTGGTCC GAGAGGGAGA CGATGGACGC
    TGGTTTGTTC GTAACCTGCG GACCAGCAAA GAGGGCTGGG TTCCTGCTGC AAACCTGCTC
    ACGCTCATCG GAGAGTCCAA ATCTTCTCAG TCTCTCTCCA GCTCAGAAGG CAGTGGGTCT
    GGAAACCTCA GTACATCGTC CAGCTGCAGT GAAACCTACA CAAGCTTCTC GGACATTAAA
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332781.1
    CDS286..3951
    Translation

    Target ORF information:

    RefSeq Version XM_021477106.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477106.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGAGACT GTCGTCAGTC CCAACACTGA GAGAGCTGCA
    GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC TTCAGTAACC TCAAGAGTCA AAAAGGATCA
    TCCCCCCCTA GTCCCGACCA CAAAACTAAA AGACAGAGTG ATCCTACACC GTTTGGCTTC
    AGAGACGCAG GTCCTCACAT CACTCTCACC CGAGTGAGAT GGCTGAGCAC TTCTAGTCTG
    TTGCAGACTA AACGCAGAGG CTTCACCAAG GCGTCGCTGT CTATGGATGC GTCTGAAGAA
    AACGACGGAT ACTCGAGCGC CGAGGACCCC ATGAACTCTG ACCCTGAGGA TGAGGGAGGG
    AAGAAATTGT CTCCAGGCAA GTACGTAGTG GTTGCTGACC ATGACAAAAG TGGACCACAG
    GAGCTGACTG TTAAGAGCGG AGACACTGTG CAACTGGTCC GAGAGGGAGA CGATGGACGC
    TGGTTTGTTC GTAACCTGCG GACCAGCAAA GAGGGCTGGG TTCCTGCTGC AAACCTGCTC
    ACGCTCATCG GAGAGTCCAA ATCTTCTCAG TCTCTCTCCA GCTCAGAAGG CAGTGGGTCT
    GGAAACCTCA GTACATCGTC CAGCTGCAGT GAAACCTACA CAAGCTTCTC GGACATTAAA
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147441
    Clone ID Related Accession (Same CDS sequence) XM_021477110.1
    Accession Version XM_021477110.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATTCTGA TGGAGGACAC GGGGTCGCAC CAGCAGCCCC CGGAGAACCT GGTGAAGATC 
    CCTTTAGAGG AGATGGAAAG TTTCTACCGG TTCCTTGTTT CCTCTGATGG GCTGATCTAT
    GAGATTATGC AGCAGGAGAG CAGTCCTCTG TGTGCCGCAG ACATCAGCCC AGACCTCAGG
    AAACAGTTTG CCTTCTTGTC TGGTGGCCGA GGACAGAACG GCAGCCCCAT CATTATTTTC
    CCAGAGTACC CGGAGTTTGG TGAGCTGGAG GAGCAGGAGT TTCACAATGT GCTGACATAC
    CTGACCAGCA TTCCCAGTCT CAGCTCGACG GGGATCGGCT TCATCCTGGT CATTGACCGC
    CGGCAAGACA GATGGGCATC AGTGAAGGGG ACACTGCTGC GGATCGCGGG CTCTTTCCCA
    GGCAACCTGC AGCTGGTGCT GGTACTGAGA CCCACAACAC TCTTCCAGCG CACAATCTCA
    GACATATTAT TTAAATTCAA CAAAGATGAC TTTAAAATGA AGGTGCCGGT GATCATGCTC
    AGCTCTGTGA CTGAGCTCCA CAGTTACATC GACCGCTCTC AGCTCACTCA GGAACTGGGC
    GGCACGCAGG AATACTGCCA CGAGAAGTGG ATTTCACACC GCACCGCCAT TGAGGGTTTT
    GCTCTGATGG TGAAGAAAAC AGCACAGACT TTGCAGTCTT TCGGGACGGA GCTGGCGGAG
    ACTGAACTGC CTAATGATGT TGAGGCCACG TCCAACCTTC TTACCGTCCA CACCGAGAAG
    AAGGACAAAA TGAAGGAGGA CCTCAGGATC GCTCTCTGTC AGGGAGGACG TTTGCTGGAG
    AGCATCAATG AGCCGCTGGT GAAAGATCCA GATTATAGCA TGAACCACGA TGAACTGGAG
    AACCTGGCTA CTGTTCAGAG GTTGCTCGGT CAGCTAGATG AGACTGAAAC GGCATTTGAT
    GATTTCTGGG ATAAACATCA GACCAAGCTG GAGCAATGTC TGCAACTGCG GCACTTTGAG
    CAGAACTTCC GGGAGGTTAG AGCTCACCTA GACCTGGTTT ACGACCGACT ATCAGGATTC
    TCCGAAGTAG GAATCAGCCC GGCCCATGCT GAACACATCC TCAAAGAACT GACCAATCAC
    GAAGAGAGAG CATCCGAGGT GATGGACCAG GCTCTGTCTC TTGCGAGTGA TGCAGATCAG
    CTGATTGAAG CGTCTCATTA TGCTGTGGAC TCCATCCTGC CCAAATGCTC TGAGCTGCGC
    TCTGTGTGTG AGGAGATCAG CACCATCCTG AGGGCCAAGA AAGCCTATTT GTTCAAATCC
    GTGGAGCTTC ACCAGTGTCT GGAGAAGGCC ACTAAATGGT ATGATGATGG AATCTACCTG
    CTGGCGTCTC AGCCTGTGGA TAAGTGTCAG TCACAGGATG GGGCGGAGTC AGCGCTGCAA
    GAGATTGAAC GTTACTTGGA TACAGCCAAT CAGCACAAGC TAACAGATTT GAATGGAATT
    TGGAGGGACT ATGATTCCGT ACTCACACAA GACTTCAGGG ATCAGGTTGA CAAAGTGTTT
    CAGAAGCAGC TGTCCATGCA GGAAATGTTT GAGAAGAGAA GGGTCAGTCT GAAGAAGCTA
    GCAGCTAAAC AAACTCGACC CGTGCAGCCT GTCGCTCCAC GTCCTGAAGC CATCATCAAA
    ACACCAGTCT CATCTCCAGC TCATAGAGAG AAGTCTATAG AAGGCGACGT CATCAATGGA
    AACTGCAAAA AAGGAGAGCT GCATTTACAG AACGAGGGCA GCAGCAGACA CCCGTCCGTC
    TCTGATGAAG AGGAGAACTT CGCTGTGCTC AGACGGCACG TGATGAACGA GCTGCTGGAG
    ACAGAGAGAG CGTATGTGGA GGAGCTGCTG TGCGTGTTGG AGGGTTATGC AGCAGAAATG
    GACAATCCTG CCATGTCTCA CCTCATCCCC AACACACTGC TTCACAAGAA GGACATCCTC
    TTCGGAAACA TGTCTGAGAT CTACCAGTTC CACAAGAAGA CTTTTCTTCG TGAACTGGAG
    GCTTACACAG ATTATCCTGA GCTTGTGGGA CAGTGTTTCT TGGAAAGAAT GACAGATCTG
    CAGATTTATG AGAAGTACTG TCAGAACAAA CCTCGCTCAG AGTCTTTATG GAGACAGTGC
    TCTGACTGCG CTTTCTTCCA GGAGTGTCAA AAGAAACTGG AACATAAGCT TGGTCTGGAC
    TCATACCTGC TGAAACCTGT GCAGAGAATA ACCAAATACC AGCTTTTACT AAAGGAGCTG
    CTGAAGTACA GTAAAGGCTG TGAGGGTGAG GAGGACCTAC AGGAGGCGCT CTCATCCATT
    CTTGGCATCC TAAAAGCTGT GAACGACTCT ATGCACCTCA TCGCCATCAC TGGATACGAA
    GGTAACCTCA GTGATCTGGG CCGTCTGATG ATGCAGGGCT CCTTCAGCGT GTGGACGGAG
    CATAAGAAGG GTCACGCCAA GGTAAAAGAC CTGGCACGAT TCAAGCCCAT GCAGAGACAC
    CTGTTCCTGC ACGAGAAAGC TCTGCTCTTC TGTAAGAAAC GAGAGGAGAA CGGGGAGGGT
    TATGAGAAAG CCCCGTCATA CAGCTTCAAA CACTCGCTCA GTATGAGTGC TGTGGGTATT
    ACAGAGAACG CCAAAGGAGA CAACAAAAAG TTTGAGATCT GGTGTAATTC TCGAGAGGAA
    GTGTATATTG TGCAGGCATC AACACCAGAG ATCAAAACAG CATGGGTGAA TGAGATCCGG
    AAGGTTCTGA CAAGGCAGCT AAAGGCCTGC AGGGAGGCGA GCCAGCAGAA GAGCTCCGAT
    CAGATCTCCC CATTAACTCC CAGCAGCACC GGCCTGACCC TGAGCCCCTT CAAAACAAAC
    CCAAAGAGCC TGAAGAAAGG AGACGAGAAG AAAACAGAAC CCACGACCAC AGATCCCAGC
    TCAGCCGCCT TATTCAAAAA CAACGACAAG CTCAAAGACG AGACTGTCGT CAGTCCCAAC
    ACTGAGAGAG CTGCAGTGGC TAAAAAGCGC TTTACACTGC AGGGCTTCAG TAACCTCAAG
    AGTCAAAAAG GATCATCCCC CCCTAGTCCC GACCACAAAA CTAAAAGACA GAGTGATCCT
    ACACCGTTTG GCTTCAGAGA CGCAGGTCCT CACATCACTC TCACCCGAGT GAGATGGCTG
    AGCACTTCTA GTCTGTTGCA GACTAAACGC AGAGGCTTCA CCAAGGCGTC GCTGTCTATG
    GATGCGTCTG AAGAAAACGA CGGATACTCG AGCGCCGAGG ACCCCATGAA CTCTGACCCT
    GAGGATGAGG GAGGGAAGAA ATTGTCTCCA GGCAAGTACG TAGTGGTTGC TGACCATGAC
    AAAAGTGGAC CACAGGAGCT GACTGTTAAG AGCGGAGACA CTGTGCAACT GGTCCGAGAG
    GGAGACGATG GACGCTGGTT TGTTCGTAAC CTGCGGACCA GCAAAGAGGG CTGGGTTCCT
    GCTGCAAACC TGCTCACGCT CATCGGAGAG TCCAAATCTT CTCAGTCTCT CTCCAGCTCA
    GAAGGCAGTG GGTCTGGAAA CCTCAGTACA TCGTCCAGCT GCAGTGAAAC CTACACAAGC
    TTCTCGGACA TTAAAGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332785.1
    CDS57..3677
    Translation

    Target ORF information:

    RefSeq Version XM_021477110.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477110.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATTCTGA TGGAGGACAC GGGGTCGCAC CAGCAGCCCC CGGAGAACCT GGTGAAGATC 
    CCTTTAGAGG AGATGGAAAG TTTCTACCGG TTCCTTGTTT CCTCTGATGG GCTGATCTAT
    GAGATTATGC AGCAGGAGAG CAGTCCTCTG TGTGCCGCAG ACATCAGCCC AGACCTCAGG
    AAACAGTTTG CCTTCTTGTC TGGTGGCCGA GGACAGAACG GCAGCCCCAT CATTATTTTC
    CCAGAGTACC CGGAGTTTGG TGAGCTGGAG GAGCAGGAGT TTCACAATGT GCTGACATAC
    CTGACCAGCA TTCCCAGTCT CAGCTCGACG GGGATCGGCT TCATCCTGGT CATTGACCGC
    CGGCAAGACA GATGGGCATC AGTGAAGGGG ACACTGCTGC GGATCGCGGG CTCTTTCCCA
    GGCAACCTGC AGCTGGTGCT GGTACTGAGA CCCACAACAC TCTTCCAGCG CACAATCTCA
    GACATATTAT TTAAATTCAA CAAAGATGAC TTTAAAATGA AGGTGCCGGT GATCATGCTC
    AGCTCTGTGA CTGAGCTCCA CAGTTACATC GACCGCTCTC AGCTCACTCA GGAACTGGGC
    GGCACGCAGG AATACTGCCA CGAGAAGTGG ATTTCACACC GCACCGCCAT TGAGGGTTTT
    GCTCTGATGG TGAAGAAAAC AGCACAGACT TTGCAGTCTT TCGGGACGGA GCTGGCGGAG
    ACTGAACTGC CTAATGATGT TGAGGCCACG TCCAACCTTC TTACCGTCCA CACCGAGAAG
    AAGGACAAAA TGAAGGAGGA CCTCAGGATC GCTCTCTGTC AGGGAGGACG TTTGCTGGAG
    AGCATCAATG AGCCGCTGGT GAAAGATCCA GATTATAGCA TGAACCACGA TGAACTGGAG
    AACCTGGCTA CTGTTCAGAG GTTGCTCGGT CAGCTAGATG AGACTGAAAC GGCATTTGAT
    GATTTCTGGG ATAAACATCA GACCAAGCTG GAGCAATGTC TGCAACTGCG GCACTTTGAG
    CAGAACTTCC GGGAGGTTAG AGCTCACCTA GACCTGGTTT ACGACCGACT ATCAGGATTC
    TCCGAAGTAG GAATCAGCCC GGCCCATGCT GAACACATCC TCAAAGAACT GACCAATCAC
    GAAGAGAGAG CATCCGAGGT GATGGACCAG GCTCTGTCTC TTGCGAGTGA TGCAGATCAG
    CTGATTGAAG CGTCTCATTA TGCTGTGGAC TCCATCCTGC CCAAATGCTC TGAGCTGCGC
    TCTGTGTGTG AGGAGATCAG CACCATCCTG AGGGCCAAGA AAGCCTATTT GTTCAAATCC
    GTGGAGCTTC ACCAGTGTCT GGAGAAGGCC ACTAAATGGT ATGATGATGG AATCTACCTG
    CTGGCGTCTC AGCCTGTGGA TAAGTGTCAG TCACAGGATG GGGCGGAGTC AGCGCTGCAA
    GAGATTGAAC GTTACTTGGA TACAGCCAAT CAGCACAAGC TAACAGATTT GAATGGAATT
    TGGAGGGACT ATGATTCCGT ACTCACACAA GACTTCAGGG ATCAGGTTGA CAAAGTGTTT
    CAGAAGCAGC TGTCCATGCA GGAAATGTTT GAGAAGAGAA GGGTCAGTCT GAAGAAGCTA
    GCAGCTAAAC AAACTCGACC CGTGCAGCCT GTCGCTCCAC GTCCTGAAGC CATCATCAAA
    ACACCAGTCT CATCTCCAGC TCATAGAGAG AAGTCTATAG AAGGCGACGT CATCAATGGA
    AACTGCAAAA AAGGAGAGCT GCATTTACAG AACGAGGGCA GCAGCAGACA CCCGTCCGTC
    TCTGATGAAG AGGAGAACTT CGCTGTGCTC AGACGGCACG TGATGAACGA GCTGCTGGAG
    ACAGAGAGAG CGTATGTGGA GGAGCTGCTG TGCGTGTTGG AGGGTTATGC AGCAGAAATG
    GACAATCCTG CCATGTCTCA CCTCATCCCC AACACACTGC TTCACAAGAA GGACATCCTC
    TTCGGAAACA TGTCTGAGAT CTACCAGTTC CACAAGAAGA CTTTTCTTCG TGAACTGGAG
    GCTTACACAG ATTATCCTGA GCTTGTGGGA CAGTGTTTCT TGGAAAGAAT GACAGATCTG
    CAGATTTATG AGAAGTACTG TCAGAACAAA CCTCGCTCAG AGTCTTTATG GAGACAGTGC
    TCTGACTGCG CTTTCTTCCA GGAGTGTCAA AAGAAACTGG AACATAAGCT TGGTCTGGAC
    TCATACCTGC TGAAACCTGT GCAGAGAATA ACCAAATACC AGCTTTTACT AAAGGAGCTG
    CTGAAGTACA GTAAAGGCTG TGAGGGTGAG GAGGACCTAC AGGAGGCGCT CTCATCCATT
    CTTGGCATCC TAAAAGCTGT GAACGACTCT ATGCACCTCA TCGCCATCAC TGGATACGAA
    GGTAACCTCA GTGATCTGGG CCGTCTGATG ATGCAGGGCT CCTTCAGCGT GTGGACGGAG
    CATAAGAAGG GTCACGCCAA GGTAAAAGAC CTGGCACGAT TCAAGCCCAT GCAGAGACAC
    CTGTTCCTGC ACGAGAAAGC TCTGCTCTTC TGTAAGAAAC GAGAGGAGAA CGGGGAGGGT
    TATGAGAAAG CCCCGTCATA CAGCTTCAAA CACTCGCTCA GTATGAGTGC TGTGGGTATT
    ACAGAGAACG CCAAAGGAGA CAACAAAAAG TTTGAGATCT GGTGTAATTC TCGAGAGGAA
    GTGTATATTG TGCAGGCATC AACACCAGAG ATCAAAACAG CATGGGTGAA TGAGATCCGG
    AAGGTTCTGA CAAGGCAGCT AAAGGCCTGC AGGGAGGCGA GCCAGCAGAA GAGCTCCGAT
    CAGATCTCCC CATTAACTCC CAGCAGCACC GGCCTGACCC TGAGCCCCTT CAAAACAAAC
    CCAAAGAGCC TGAAGAAAGG AGACGAGAAG AAAACAGAAC CCACGACCAC AGATCCCAGC
    TCAGCCGCCT TATTCAAAAA CAACGACAAG CTCAAAGACG AGACTGTCGT CAGTCCCAAC
    ACTGAGAGAG CTGCAGTGGC TAAAAAGCGC TTTACACTGC AGGGCTTCAG TAACCTCAAG
    AGTCAAAAAG GATCATCCCC CCCTAGTCCC GACCACAAAA CTAAAAGACA GAGTGATCCT
    ACACCGTTTG GCTTCAGAGA CGCAGGTCCT CACATCACTC TCACCCGAGT GAGATGGCTG
    AGCACTTCTA GTCTGTTGCA GACTAAACGC AGAGGCTTCA CCAAGGCGTC GCTGTCTATG
    GATGCGTCTG AAGAAAACGA CGGATACTCG AGCGCCGAGG ACCCCATGAA CTCTGACCCT
    GAGGATGAGG GAGGGAAGAA ATTGTCTCCA GGCAAGTACG TAGTGGTTGC TGACCATGAC
    AAAAGTGGAC CACAGGAGCT GACTGTTAAG AGCGGAGACA CTGTGCAACT GGTCCGAGAG
    GGAGACGATG GACGCTGGTT TGTTCGTAAC CTGCGGACCA GCAAAGAGGG CTGGGTTCCT
    GCTGCAAACC TGCTCACGCT CATCGGAGAG TCCAAATCTT CTCAGTCTCT CTCCAGCTCA
    GAAGGCAGTG GGTCTGGAAA CCTCAGTACA TCGTCCAGCT GCAGTGAAAC CTACACAAGC
    TTCTCGGACA TTAAAGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147442
    Clone ID Related Accession (Same CDS sequence) XM_021477116.1
    Accession Version XM_021477116.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGCTCA ACCGAGTGTC CCTGCTCTGT CAGGACATTA CCAAACTCTG GCTGCAGCTC 
    AAAGTCTTGA CAGATGAGAT TATGCAGCAG GAGAGCAGTC CTCTGTGTGC CGCAGACATC
    AGCCCAGACC TCAGGAAACA GTTTGCCTTC TTGTCTGGTG GCCGAGGACA GAACGGCAGC
    CCCATCATTA TTTTCCCAGA GTACCCGGAG TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC
    AATGTGCTGA CATACCTGAC CAGCATTCCC AGTCTCAGCT CGACGGGGAT CGGCTTCATC
    CTGGTCATTG ACCGCCGGCA AGACAGATGG GCATCAGTGA AGGGGACACT GCTGCGGATC
    GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG GTGCTGGTAC TGAGACCCAC AACACTCTTC
    CAGCGCACAA TCTCAGACAT ATTATTTAAA TTCAACAAAG ATGACTTTAA AATGAAGGTG
    CCGGTGATCA TGCTCAGCTC TGTGACTGAG CTCCACAGTT ACATCGACCG CTCTCAGCTC
    ACTCAGGAAC TGGGCGGCAC GCAGGAATAC TGCCACGAGA AGTGGATTTC ACACCGCACC
    GCCATTGAGG GTTTTGCTCT GATGGTGAAG AAAACAGCAC AGACTTTGCA GTCTTTCGGG
    ACGGAGCTGG CGGAGACTGA ACTGCCTAAT GATGTTGAGG CCACGTCCAA CCTTCTTACC
    GTCCACACCG AGAAGAAGGA CAAAATGAAG GAGGACCTCA GGATCGCTCT CTGTCAGGGA
    GGACGTTTGC TGGAGAGCAT CAATGAGCCG CTGGTGAAAG ATCCAGATTA TAGCATGAAC
    CACGATGAAC TGGAGAACCT GGCTACTGTT CAGAGGTTGC TCGGTCAGCT AGATGAGACT
    GAAACGGCAT TTGATGATTT CTGGGATAAA CATCAGACCA AGCTGGAGCA ATGTCTGCAA
    CTGCGGCACT TTGAGCAGAA CTTCCGGGAG GTTAGAGCTC ACCTAGACCT GGTTTACGAC
    CGACTATCAG GATTCTCCGA AGTAGGAATC AGCCCGGCCC ATGCTGAACA CATCCTCAAA
    GAACTGACCA ATCACGAAGA GAGAGCATCC GAGGTGATGG ACCAGGCTCT GTCTCTTGCG
    AGTGATGCAG ATCAGCTGAT TGAAGCGTCT CATTATGCTG TGGACTCCAT CCTGCCCAAA
    TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG ATCAGCACCA TCCTGAGGGC CAAGAAAGCC
    TATTTGTTCA AATCCGTGGA GCTTCACCAG TGTCTGGAGA AGGCCACTAA ATGGTATGAT
    GATGGAATCT ACCTGCTGGC GTCTCAGCCT GTGGATAAGT GTCAGTCACA GGATGGGGCG
    GAGTCAGCGC TGCAAGAGAT TGAACGTTAC TTGGATACAG CCAATCAGCA CAAGCTAACA
    GATTTGAATG GAATTTGGAG GGACTATGAT TCCGTACTCA CACAAGACTT CAGGGATCAG
    GTTGACAAAG TGTTTCAGAA GCAGCTGTCC ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC
    AGTCTGAAGA AGCTAGCAGC TAAACAAACT CGACCCGTGC AGCCTGTCGC TCCACGTCCT
    GAAGCCATCA TCAAAACACC AGTCTCATCT CCAGCTCATA GAGAGAAGTC TATAGAAGGC
    GACGTCATCA ATGGAAACTG CAAAAAAGGA GAGCTGCATT TACAGAACGA GGGCAGCAGC
    AGACACCCGT CCGTCTCTGA TGAAGAGGAG AACTTCGCTG TGCTCAGACG GCACGTGATG
    AACGAGCTGC TGGAGACAGA GAGAGCGTAT GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT
    TATGCAGCAG AAATGGACAA TCCTGCCATG TCTCACCTCA TCCCCAACAC ACTGCTTCAC
    AAGAAGGACA TCCTCTTCGG AAACATGTCT GAGATCTACC AGTTCCACAA GAAGACTTTT
    CTTCGTGAAC TGGAGGCTTA CACAGATTAT CCTGAGCTTG TGGGACAGTG TTTCTTGGAA
    AGAATGACAG ATCTGCAGAT TTATGAGAAG TACTGTCAGA ACAAACCTCG CTCAGAGTCT
    TTATGGAGAC AGTGCTCTGA CTGCGCTTTC TTCCAGGAGT GTCAAAAGAA ACTGGAACAT
    AAGCTTGGTC TGGACTCATA CCTGCTGAAA CCTGTGCAGA GAATAACCAA ATACCAGCTT
    TTACTAAAGG AGCTGCTGAA GTACAGTAAA GGCTGTGAGG GTGAGGAGGA CCTACAGGAG
    GCGCTCTCAT CCATTCTTGG CATCCTAAAA GCTGTGAACG ACTCTATGCA CCTCATCGCC
    ATCACTGGAT ACGAAGGTAA CCTCAGTGAT CTGGGCCGTC TGATGATGCA GGGCTCCTTC
    AGCGTGTGGA CGGAGCATAA GAAGGGTCAC GCCAAGGTAA AAGACCTGGC ACGATTCAAG
    CCCATGCAGA GACACCTGTT CCTGCACGAG AAAGCTCTGC TCTTCTGTAA GAAACGAGAG
    GAGAACGGGG AGGGTTATGA GAAAGCCCCG TCATACAGCT TCAAACACTC GCTCAGTATG
    AGTGCTGTGG GTATTACAGA GAACGCCAAA GGAGACAACA AAAAGTTTGA GATCTGGTGT
    AATTCTCGAG AGGAAGTGTA TATTGTGCAG GCATCAACAC CAGAGATCAA AACAGCATGG
    GTGAATGAGA TCCGGAAGGT TCTGACAAGG CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG
    CAGAAGAGCT CCGATCAGAT CTCCCCATTA ACTCCCAGCA GCACCGGCCT GACCCTGAGC
    CCCTTCAAAA CAAACCCAAA GAGCCTGAAG AAAGGAGACG AGAAGAAAAC AGAACCCACG
    ACCACAGATC CCAGCTCAGC CGCCTTATTC AAAAACAACG ACAAGCTCAA AGACGAGACT
    GTCGTCAGTC CCAACACTGA GAGAGCTGCA GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC
    TTCAGTAACC TCAAGAGTCA AAAAGGATCA TCCCCCCCTA GTCCCGACCA CAAAACTAAA
    AGACAGAGTG ATCCTACACC GTTTGGCTTC AGAGACGCAG GTCCTCACAT CACTCTCACC
    CGAGTGAGAT GGCTGAGCAC TTCTAGTCTG TTGCAGACTA AACGCAGAGG CTTCACCAAG
    GCGTCGCTGT CTATGGATGC GTCTGAAGAA AACGACGGAT ACTCGAGCGC CGAGGACCCC
    ATGAACTCTG ACCCTGAGGA TGAGGGAGGG AAGAAATTGT CTCCAGGCAA GTACGTAGTG
    GTTGCTGACC ATGACAAAAG TGGACCACAG GAGCTGACTG TTAAGAGCGG AGACACTGTG
    CAACTGGTCC GAGAGGGAGA CGATGGACGC TGGTTTGTTC GTAACCTGCG GACCAGCAAA
    GAGGGCTGGG TTCCTGCTGC AAACCTGCTC ACGCTCATCG GAGAGTCCAA ATCTTCTCAG
    TCTCTCTCCA GCTCAGAAGG CAGTGGGTCT GGAAACCTCA GTACATCGTC CAGCTGCAGT
    GAAACCTACA CAAGCTTCTC GGACATTAAA GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332791.1
    CDS359..3934
    Translation

    Target ORF information:

    RefSeq Version XM_021477116.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477116.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGCTCA ACCGAGTGTC CCTGCTCTGT CAGGACATTA CCAAACTCTG GCTGCAGCTC 
    AAAGTCTTGA CAGATGAGAT TATGCAGCAG GAGAGCAGTC CTCTGTGTGC CGCAGACATC
    AGCCCAGACC TCAGGAAACA GTTTGCCTTC TTGTCTGGTG GCCGAGGACA GAACGGCAGC
    CCCATCATTA TTTTCCCAGA GTACCCGGAG TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC
    AATGTGCTGA CATACCTGAC CAGCATTCCC AGTCTCAGCT CGACGGGGAT CGGCTTCATC
    CTGGTCATTG ACCGCCGGCA AGACAGATGG GCATCAGTGA AGGGGACACT GCTGCGGATC
    GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG GTGCTGGTAC TGAGACCCAC AACACTCTTC
    CAGCGCACAA TCTCAGACAT ATTATTTAAA TTCAACAAAG ATGACTTTAA AATGAAGGTG
    CCGGTGATCA TGCTCAGCTC TGTGACTGAG CTCCACAGTT ACATCGACCG CTCTCAGCTC
    ACTCAGGAAC TGGGCGGCAC GCAGGAATAC TGCCACGAGA AGTGGATTTC ACACCGCACC
    GCCATTGAGG GTTTTGCTCT GATGGTGAAG AAAACAGCAC AGACTTTGCA GTCTTTCGGG
    ACGGAGCTGG CGGAGACTGA ACTGCCTAAT GATGTTGAGG CCACGTCCAA CCTTCTTACC
    GTCCACACCG AGAAGAAGGA CAAAATGAAG GAGGACCTCA GGATCGCTCT CTGTCAGGGA
    GGACGTTTGC TGGAGAGCAT CAATGAGCCG CTGGTGAAAG ATCCAGATTA TAGCATGAAC
    CACGATGAAC TGGAGAACCT GGCTACTGTT CAGAGGTTGC TCGGTCAGCT AGATGAGACT
    GAAACGGCAT TTGATGATTT CTGGGATAAA CATCAGACCA AGCTGGAGCA ATGTCTGCAA
    CTGCGGCACT TTGAGCAGAA CTTCCGGGAG GTTAGAGCTC ACCTAGACCT GGTTTACGAC
    CGACTATCAG GATTCTCCGA AGTAGGAATC AGCCCGGCCC ATGCTGAACA CATCCTCAAA
    GAACTGACCA ATCACGAAGA GAGAGCATCC GAGGTGATGG ACCAGGCTCT GTCTCTTGCG
    AGTGATGCAG ATCAGCTGAT TGAAGCGTCT CATTATGCTG TGGACTCCAT CCTGCCCAAA
    TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG ATCAGCACCA TCCTGAGGGC CAAGAAAGCC
    TATTTGTTCA AATCCGTGGA GCTTCACCAG TGTCTGGAGA AGGCCACTAA ATGGTATGAT
    GATGGAATCT ACCTGCTGGC GTCTCAGCCT GTGGATAAGT GTCAGTCACA GGATGGGGCG
    GAGTCAGCGC TGCAAGAGAT TGAACGTTAC TTGGATACAG CCAATCAGCA CAAGCTAACA
    GATTTGAATG GAATTTGGAG GGACTATGAT TCCGTACTCA CACAAGACTT CAGGGATCAG
    GTTGACAAAG TGTTTCAGAA GCAGCTGTCC ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC
    AGTCTGAAGA AGCTAGCAGC TAAACAAACT CGACCCGTGC AGCCTGTCGC TCCACGTCCT
    GAAGCCATCA TCAAAACACC AGTCTCATCT CCAGCTCATA GAGAGAAGTC TATAGAAGGC
    GACGTCATCA ATGGAAACTG CAAAAAAGGA GAGCTGCATT TACAGAACGA GGGCAGCAGC
    AGACACCCGT CCGTCTCTGA TGAAGAGGAG AACTTCGCTG TGCTCAGACG GCACGTGATG
    AACGAGCTGC TGGAGACAGA GAGAGCGTAT GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT
    TATGCAGCAG AAATGGACAA TCCTGCCATG TCTCACCTCA TCCCCAACAC ACTGCTTCAC
    AAGAAGGACA TCCTCTTCGG AAACATGTCT GAGATCTACC AGTTCCACAA GAAGACTTTT
    CTTCGTGAAC TGGAGGCTTA CACAGATTAT CCTGAGCTTG TGGGACAGTG TTTCTTGGAA
    AGAATGACAG ATCTGCAGAT TTATGAGAAG TACTGTCAGA ACAAACCTCG CTCAGAGTCT
    TTATGGAGAC AGTGCTCTGA CTGCGCTTTC TTCCAGGAGT GTCAAAAGAA ACTGGAACAT
    AAGCTTGGTC TGGACTCATA CCTGCTGAAA CCTGTGCAGA GAATAACCAA ATACCAGCTT
    TTACTAAAGG AGCTGCTGAA GTACAGTAAA GGCTGTGAGG GTGAGGAGGA CCTACAGGAG
    GCGCTCTCAT CCATTCTTGG CATCCTAAAA GCTGTGAACG ACTCTATGCA CCTCATCGCC
    ATCACTGGAT ACGAAGGTAA CCTCAGTGAT CTGGGCCGTC TGATGATGCA GGGCTCCTTC
    AGCGTGTGGA CGGAGCATAA GAAGGGTCAC GCCAAGGTAA AAGACCTGGC ACGATTCAAG
    CCCATGCAGA GACACCTGTT CCTGCACGAG AAAGCTCTGC TCTTCTGTAA GAAACGAGAG
    GAGAACGGGG AGGGTTATGA GAAAGCCCCG TCATACAGCT TCAAACACTC GCTCAGTATG
    AGTGCTGTGG GTATTACAGA GAACGCCAAA GGAGACAACA AAAAGTTTGA GATCTGGTGT
    AATTCTCGAG AGGAAGTGTA TATTGTGCAG GCATCAACAC CAGAGATCAA AACAGCATGG
    GTGAATGAGA TCCGGAAGGT TCTGACAAGG CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG
    CAGAAGAGCT CCGATCAGAT CTCCCCATTA ACTCCCAGCA GCACCGGCCT GACCCTGAGC
    CCCTTCAAAA CAAACCCAAA GAGCCTGAAG AAAGGAGACG AGAAGAAAAC AGAACCCACG
    ACCACAGATC CCAGCTCAGC CGCCTTATTC AAAAACAACG ACAAGCTCAA AGACGAGACT
    GTCGTCAGTC CCAACACTGA GAGAGCTGCA GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC
    TTCAGTAACC TCAAGAGTCA AAAAGGATCA TCCCCCCCTA GTCCCGACCA CAAAACTAAA
    AGACAGAGTG ATCCTACACC GTTTGGCTTC AGAGACGCAG GTCCTCACAT CACTCTCACC
    CGAGTGAGAT GGCTGAGCAC TTCTAGTCTG TTGCAGACTA AACGCAGAGG CTTCACCAAG
    GCGTCGCTGT CTATGGATGC GTCTGAAGAA AACGACGGAT ACTCGAGCGC CGAGGACCCC
    ATGAACTCTG ACCCTGAGGA TGAGGGAGGG AAGAAATTGT CTCCAGGCAA GTACGTAGTG
    GTTGCTGACC ATGACAAAAG TGGACCACAG GAGCTGACTG TTAAGAGCGG AGACACTGTG
    CAACTGGTCC GAGAGGGAGA CGATGGACGC TGGTTTGTTC GTAACCTGCG GACCAGCAAA
    GAGGGCTGGG TTCCTGCTGC AAACCTGCTC ACGCTCATCG GAGAGTCCAA ATCTTCTCAG
    TCTCTCTCCA GCTCAGAAGG CAGTGGGTCT GGAAACCTCA GTACATCGTC CAGCTGCAGT
    GAAACCTACA CAAGCTTCTC GGACATTAAA GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147443
    Clone ID Related Accession (Same CDS sequence) XM_021477126.1
    Accession Version XM_021477126.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAACGT TGTGCTGTTT CCTTCACAGA AATGGCAGGA CACAAACCAG GTTCACGCAG 
    ATCTCAAGAT CAGATGAGAT TATGCAGCAG GAGAGCAGTC CTCTGTGTGC CGCAGACATC
    AGCCCAGACC TCAGGAAACA GTTTGCCTTC TTGTCTGGTG GCCGAGGACA GAACGGCAGC
    CCCATCATTA TTTTCCCAGA GTACCCGGAG TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC
    AATGTGCTGA CATACCTGAC CAGCATTCCC AGTCTCAGCT CGACGGGGAT CGGCTTCATC
    CTGGTCATTG ACCGCCGGCA AGACAGATGG GCATCAGTGA AGGGGACACT GCTGCGGATC
    GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG GTGCTGGTAC TGAGACCCAC AACACTCTTC
    CAGCGCACAA TCTCAGACAT ATTATTTAAA TTCAACAAAG ATGACTTTAA AATGAAGGTG
    CCGGTGATCA TGCTCAGCTC TGTGACTGAG CTCCACAGTT ACATCGACCG CTCTCAGCTC
    ACTCAGGAAC TGGGCGGCAC GCAGGAATAC TGCCACGAGA AGTGGATTTC ACACCGCACC
    GCCATTGAGG GTTTTGCTCT GATGGTGAAG AAAACAGCAC AGACTTTGCA GTCTTTCGGG
    ACGGAGCTGG CGGAGACTGA ACTGCCTAAT GATGTTGAGG CCACGTCCAA CCTTCTTACC
    GTCCACACCG AGAAGAAGGA CAAAATGAAG GAGGACCTCA GGATCGCTCT CTGTCAGGGA
    GGACGTTTGC TGGAGAGCAT CAATGAGCCG CTGGTGAAAG ATCCAGATTA TAGCATGAAC
    CACGATGAAC TGGAGAACCT GGCTACTGTT CAGAGGTTGC TCGGTCAGCT AGATGAGACT
    GAAACGGCAT TTGATGATTT CTGGGATAAA CATCAGACCA AGCTGGAGCA ATGTCTGCAA
    CTGCGGCACT TTGAGCAGAA CTTCCGGGAG GTTAGAGCTC ACCTAGACCT GGTTTACGAC
    CGACTATCAG GATTCTCCGA AGTAGGAATC AGCCCGGCCC ATGCTGAACA CATCCTCAAA
    GAACTGACCA ATCACGAAGA GAGAGCATCC GAGGTGATGG ACCAGGCTCT GTCTCTTGCG
    AGTGATGCAG ATCAGCTGAT TGAAGCGTCT CATTATGCTG TGGACTCCAT CCTGCCCAAA
    TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG ATCAGCACCA TCCTGAGGGC CAAGAAAGCC
    TATTTGTTCA AATCCGTGGA GCTTCACCAG TGTCTGGAGA AGGCCACTAA ATGGTATGAT
    GATGGAATCT ACCTGCTGGC GTCTCAGCCT GTGGATAAGT GTCAGTCACA GGATGGGGCG
    GAGTCAGCGC TGCAAGAGAT TGAACGTTAC TTGGATACAG CCAATCAGCA CAAGCTAACA
    GATTTGAATG GAATTTGGAG GGACTATGAT TCCGTACTCA CACAAGACTT CAGGGATCAG
    GTTGACAAAG TGTTTCAGAA GCAGCTGTCC ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC
    AGTCTGAAGA AGCTAGCAGC TAAACAAACT CGACCCGTGC AGCCTGTCGC TCCACGTCCT
    GAAGCCATCA TCAAAACACC AGTCTCATCT CCAGCTCATA GAGAGAAGTC TATAGAAGGC
    GACGTCATCA ATGGAAACTG CAAAAAAGGA GAGCTGCATT TACAGAACGA GGGCAGCAGC
    AGACACCCGT CCGTCTCTGA TGAAGAGGAG AACTTCGCTG TGCTCAGACG GCACGTGATG
    AACGAGCTGC TGGAGACAGA GAGAGCGTAT GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT
    TATGCAGCAG AAATGGACAA TCCTGCCATG TCTCACCTCA TCCCCAACAC ACTGCTTCAC
    AAGAAGGACA TCCTCTTCGG AAACATGTCT GAGATCTACC AGTTCCACAA GAAGACTTTT
    CTTCGTGAAC TGGAGGCTTA CACAGATTAT CCTGAGCTTG TGGGACAGTG TTTCTTGGAA
    AGAATGACAG ATCTGCAGAT TTATGAGAAG TACTGTCAGA ACAAACCTCG CTCAGAGTCT
    TTATGGAGAC AGTGCTCTGA CTGCGCTTTC TTCCAGGAGT GTCAAAAGAA ACTGGAACAT
    AAGCTTGGTC TGGACTCATA CCTGCTGAAA CCTGTGCAGA GAATAACCAA ATACCAGCTT
    TTACTAAAGG AGCTGCTGAA GTACAGTAAA GGCTGTGAGG GTGAGGAGGA CCTACAGGAG
    GCGCTCTCAT CCATTCTTGG CATCCTAAAA GCTGTGAACG ACTCTATGCA CCTCATCGCC
    ATCACTGGAT ACGAAGGTAA CCTCAGTGAT CTGGGCCGTC TGATGATGCA GGGCTCCTTC
    AGCGTGTGGA CGGAGCATAA GAAGGGTCAC GCCAAGGTAA AAGACCTGGC ACGATTCAAG
    CCCATGCAGA GACACCTGTT CCTGCACGAG AAAGCTCTGC TCTTCTGTAA GAAACGAGAG
    GAGAACGGGG AGGGTTATGA GAAAGCCCCG TCATACAGCT TCAAACACTC GCTCAGTATG
    AGTGCTGTGG GTATTACAGA GAACGCCAAA GGAGACAACA AAAAGTTTGA GATCTGGTGT
    AATTCTCGAG AGGAAGTGTA TATTGTGCAG GCATCAACAC CAGAGATCAA AACAGCATGG
    GTGAATGAGA TCCGGAAGGT TCTGACAAGG CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG
    CAGAAGAGCT CCGATCAGAT CTCCCCATTA ACTCCCAGCA GCACCGGCCT GACCCTGAGC
    CCCTTCAAAA CAAACCCAAA GAGCCTGAAG AAAGGAGACG AGAAGAAAAC AGAACCCACG
    ACCACAGATC CCAGCTCAGC CGCCTTATTC AAAAACAACG ACAAGCTCAA AGACGAGACT
    GTCGTCAGTC CCAACACTGA GAGAGCTGCA GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC
    TTCAGTAACC TCAAGAGTCA AAAAGGATCA TCCCCCCCTA GTCCCGACCA CAAAACTAAA
    AGACAGAGTG ATCCTACACC GTTTGGCTTC AGAGACGCAG GTCCTCACAT CACTCTCACC
    CGAGTGAGAT GGCTGAGCAC TTCTAGTCTG TTGCAGACTA AACGCAGAGG CTTCACCAAG
    GCGTCGCTGT CTATGGATGC GTCTGAAGAA AACGACGGAT ACTCGAGCGC CGAGGACCCC
    ATGAACTCTG ACCCTGAGGA TGAGGGAGGG AAGAAATTGT CTCCAGGCAA GTACGTAGTG
    GTTGCTGACC ATGACAAAAG TGGACCACAG GAGCTGACTG TTAAGAGCGG AGACACTGTG
    CAACTGGTCC GAGAGGGAGA CGATGGACGC TGGTTTGTTC GTAACCTGCG GACCAGCAAA
    GAGGGCTGGG TTCCTGCTGC AAACCTGCTC ACGCTCATCG GAGAGTCCAA ATCTTCTCAG
    TCTCTCTCCA GCTCAGAAGG CAGTGGGTCT GGAAACCTCA GTACATCGTC CAGCTGCAGT
    GAAACCTACA CAAGCTTCTC GGACATTAAA GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332801.1
    CDS16..3591
    Translation

    Target ORF information:

    RefSeq Version XM_021477126.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477126.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAACGT TGTGCTGTTT CCTTCACAGA AATGGCAGGA CACAAACCAG GTTCACGCAG 
    ATCTCAAGAT CAGATGAGAT TATGCAGCAG GAGAGCAGTC CTCTGTGTGC CGCAGACATC
    AGCCCAGACC TCAGGAAACA GTTTGCCTTC TTGTCTGGTG GCCGAGGACA GAACGGCAGC
    CCCATCATTA TTTTCCCAGA GTACCCGGAG TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC
    AATGTGCTGA CATACCTGAC CAGCATTCCC AGTCTCAGCT CGACGGGGAT CGGCTTCATC
    CTGGTCATTG ACCGCCGGCA AGACAGATGG GCATCAGTGA AGGGGACACT GCTGCGGATC
    GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG GTGCTGGTAC TGAGACCCAC AACACTCTTC
    CAGCGCACAA TCTCAGACAT ATTATTTAAA TTCAACAAAG ATGACTTTAA AATGAAGGTG
    CCGGTGATCA TGCTCAGCTC TGTGACTGAG CTCCACAGTT ACATCGACCG CTCTCAGCTC
    ACTCAGGAAC TGGGCGGCAC GCAGGAATAC TGCCACGAGA AGTGGATTTC ACACCGCACC
    GCCATTGAGG GTTTTGCTCT GATGGTGAAG AAAACAGCAC AGACTTTGCA GTCTTTCGGG
    ACGGAGCTGG CGGAGACTGA ACTGCCTAAT GATGTTGAGG CCACGTCCAA CCTTCTTACC
    GTCCACACCG AGAAGAAGGA CAAAATGAAG GAGGACCTCA GGATCGCTCT CTGTCAGGGA
    GGACGTTTGC TGGAGAGCAT CAATGAGCCG CTGGTGAAAG ATCCAGATTA TAGCATGAAC
    CACGATGAAC TGGAGAACCT GGCTACTGTT CAGAGGTTGC TCGGTCAGCT AGATGAGACT
    GAAACGGCAT TTGATGATTT CTGGGATAAA CATCAGACCA AGCTGGAGCA ATGTCTGCAA
    CTGCGGCACT TTGAGCAGAA CTTCCGGGAG GTTAGAGCTC ACCTAGACCT GGTTTACGAC
    CGACTATCAG GATTCTCCGA AGTAGGAATC AGCCCGGCCC ATGCTGAACA CATCCTCAAA
    GAACTGACCA ATCACGAAGA GAGAGCATCC GAGGTGATGG ACCAGGCTCT GTCTCTTGCG
    AGTGATGCAG ATCAGCTGAT TGAAGCGTCT CATTATGCTG TGGACTCCAT CCTGCCCAAA
    TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG ATCAGCACCA TCCTGAGGGC CAAGAAAGCC
    TATTTGTTCA AATCCGTGGA GCTTCACCAG TGTCTGGAGA AGGCCACTAA ATGGTATGAT
    GATGGAATCT ACCTGCTGGC GTCTCAGCCT GTGGATAAGT GTCAGTCACA GGATGGGGCG
    GAGTCAGCGC TGCAAGAGAT TGAACGTTAC TTGGATACAG CCAATCAGCA CAAGCTAACA
    GATTTGAATG GAATTTGGAG GGACTATGAT TCCGTACTCA CACAAGACTT CAGGGATCAG
    GTTGACAAAG TGTTTCAGAA GCAGCTGTCC ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC
    AGTCTGAAGA AGCTAGCAGC TAAACAAACT CGACCCGTGC AGCCTGTCGC TCCACGTCCT
    GAAGCCATCA TCAAAACACC AGTCTCATCT CCAGCTCATA GAGAGAAGTC TATAGAAGGC
    GACGTCATCA ATGGAAACTG CAAAAAAGGA GAGCTGCATT TACAGAACGA GGGCAGCAGC
    AGACACCCGT CCGTCTCTGA TGAAGAGGAG AACTTCGCTG TGCTCAGACG GCACGTGATG
    AACGAGCTGC TGGAGACAGA GAGAGCGTAT GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT
    TATGCAGCAG AAATGGACAA TCCTGCCATG TCTCACCTCA TCCCCAACAC ACTGCTTCAC
    AAGAAGGACA TCCTCTTCGG AAACATGTCT GAGATCTACC AGTTCCACAA GAAGACTTTT
    CTTCGTGAAC TGGAGGCTTA CACAGATTAT CCTGAGCTTG TGGGACAGTG TTTCTTGGAA
    AGAATGACAG ATCTGCAGAT TTATGAGAAG TACTGTCAGA ACAAACCTCG CTCAGAGTCT
    TTATGGAGAC AGTGCTCTGA CTGCGCTTTC TTCCAGGAGT GTCAAAAGAA ACTGGAACAT
    AAGCTTGGTC TGGACTCATA CCTGCTGAAA CCTGTGCAGA GAATAACCAA ATACCAGCTT
    TTACTAAAGG AGCTGCTGAA GTACAGTAAA GGCTGTGAGG GTGAGGAGGA CCTACAGGAG
    GCGCTCTCAT CCATTCTTGG CATCCTAAAA GCTGTGAACG ACTCTATGCA CCTCATCGCC
    ATCACTGGAT ACGAAGGTAA CCTCAGTGAT CTGGGCCGTC TGATGATGCA GGGCTCCTTC
    AGCGTGTGGA CGGAGCATAA GAAGGGTCAC GCCAAGGTAA AAGACCTGGC ACGATTCAAG
    CCCATGCAGA GACACCTGTT CCTGCACGAG AAAGCTCTGC TCTTCTGTAA GAAACGAGAG
    GAGAACGGGG AGGGTTATGA GAAAGCCCCG TCATACAGCT TCAAACACTC GCTCAGTATG
    AGTGCTGTGG GTATTACAGA GAACGCCAAA GGAGACAACA AAAAGTTTGA GATCTGGTGT
    AATTCTCGAG AGGAAGTGTA TATTGTGCAG GCATCAACAC CAGAGATCAA AACAGCATGG
    GTGAATGAGA TCCGGAAGGT TCTGACAAGG CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG
    CAGAAGAGCT CCGATCAGAT CTCCCCATTA ACTCCCAGCA GCACCGGCCT GACCCTGAGC
    CCCTTCAAAA CAAACCCAAA GAGCCTGAAG AAAGGAGACG AGAAGAAAAC AGAACCCACG
    ACCACAGATC CCAGCTCAGC CGCCTTATTC AAAAACAACG ACAAGCTCAA AGACGAGACT
    GTCGTCAGTC CCAACACTGA GAGAGCTGCA GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC
    TTCAGTAACC TCAAGAGTCA AAAAGGATCA TCCCCCCCTA GTCCCGACCA CAAAACTAAA
    AGACAGAGTG ATCCTACACC GTTTGGCTTC AGAGACGCAG GTCCTCACAT CACTCTCACC
    CGAGTGAGAT GGCTGAGCAC TTCTAGTCTG TTGCAGACTA AACGCAGAGG CTTCACCAAG
    GCGTCGCTGT CTATGGATGC GTCTGAAGAA AACGACGGAT ACTCGAGCGC CGAGGACCCC
    ATGAACTCTG ACCCTGAGGA TGAGGGAGGG AAGAAATTGT CTCCAGGCAA GTACGTAGTG
    GTTGCTGACC ATGACAAAAG TGGACCACAG GAGCTGACTG TTAAGAGCGG AGACACTGTG
    CAACTGGTCC GAGAGGGAGA CGATGGACGC TGGTTTGTTC GTAACCTGCG GACCAGCAAA
    GAGGGCTGGG TTCCTGCTGC AAACCTGCTC ACGCTCATCG GAGAGTCCAA ATCTTCTCAG
    TCTCTCTCCA GCTCAGAAGG CAGTGGGTCT GGAAACCTCA GTACATCGTC CAGCTGCAGT
    GAAACCTACA CAAGCTTCTC GGACATTAAA GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147444
    Clone ID Related Accession (Same CDS sequence) XM_021477133.1
    Accession Version XM_021477133.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTCTCCT TGTTCTCATG CACCAGGAAA CACAGTCCTA GTAGAGATGA GATTATGCAG 
    CAGGAGAGCA GTCCTCTGTG TGCCGCAGAC ATCAGCCCAG ACCTCAGGAA ACAGTTTGCC
    TTCTTGTCTG GTGGCCGAGG ACAGAACGGC AGCCCCATCA TTATTTTCCC AGAGTACCCG
    GAGTTTGGTG AGCTGGAGGA GCAGGAGTTT CACAATGTGC TGACATACCT GACCAGCATT
    CCCAGTCTCA GCTCGACGGG GATCGGCTTC ATCCTGGTCA TTGACCGCCG GCAAGACAGA
    TGGGCATCAG TGAAGGGGAC ACTGCTGCGG ATCGCGGGCT CTTTCCCAGG CAACCTGCAG
    CTGGTGCTGG TACTGAGACC CACAACACTC TTCCAGCGCA CAATCTCAGA CATATTATTT
    AAATTCAACA AAGATGACTT TAAAATGAAG GTGCCGGTGA TCATGCTCAG CTCTGTGACT
    GAGCTCCACA GTTACATCGA CCGCTCTCAG CTCACTCAGG AACTGGGCGG CACGCAGGAA
    TACTGCCACG AGAAGTGGAT TTCACACCGC ACCGCCATTG AGGGTTTTGC TCTGATGGTG
    AAGAAAACAG CACAGACTTT GCAGTCTTTC GGGACGGAGC TGGCGGAGAC TGAACTGCCT
    AATGATGTTG AGGCCACGTC CAACCTTCTT ACCGTCCACA CCGAGAAGAA GGACAAAATG
    AAGGAGGACC TCAGGATCGC TCTCTGTCAG GGAGGACGTT TGCTGGAGAG CATCAATGAG
    CCGCTGGTGA AAGATCCAGA TTATAGCATG AACCACGATG AACTGGAGAA CCTGGCTACT
    GTTCAGAGGT TGCTCGGTCA GCTAGATGAG ACTGAAACGG CATTTGATGA TTTCTGGGAT
    AAACATCAGA CCAAGCTGGA GCAATGTCTG CAACTGCGGC ACTTTGAGCA GAACTTCCGG
    GAGGTTAGAG CTCACCTAGA CCTGGTTTAC GACCGACTAT CAGGATTCTC CGAAGTAGGA
    ATCAGCCCGG CCCATGCTGA ACACATCCTC AAAGAACTGA CCAATCACGA AGAGAGAGCA
    TCCGAGGTGA TGGACCAGGC TCTGTCTCTT GCGAGTGATG CAGATCAGCT GATTGAAGCG
    TCTCATTATG CTGTGGACTC CATCCTGCCC AAATGCTCTG AGCTGCGCTC TGTGTGTGAG
    GAGATCAGCA CCATCCTGAG GGCCAAGAAA GCCTATTTGT TCAAATCCGT GGAGCTTCAC
    CAGTGTCTGG AGAAGGCCAC TAAATGGTAT GATGATGGAA TCTACCTGCT GGCGTCTCAG
    CCTGTGGATA AGTGTCAGTC ACAGGATGGG GCGGAGTCAG CGCTGCAAGA GATTGAACGT
    TACTTGGATA CAGCCAATCA GCACAAGCTA ACAGATTTGA ATGGAATTTG GAGGGACTAT
    GATTCCGTAC TCACACAAGA CTTCAGGGAT CAGGTTGACA AAGTGTTTCA GAAGCAGCTG
    TCCATGCAGG AAATGTTTGA GAAGAGAAGG GTCAGTCTGA AGAAGCTAGC AGCTAAACAA
    ACTCGACCCG TGCAGCCTGT CGCTCCACGT CCTGAAGCCA TCATCAAAAC ACCAGTCTCA
    TCTCCAGCTC ATAGAGAGAA GTCTATAGAA GGCGACGTCA TCAATGGAAA CTGCAAAAAA
    GGAGAGCTGC ATTTACAGAA CGAGGGCAGC AGCAGACACC CGTCCGTCTC TGATGAAGAG
    GAGAACTTCG CTGTGCTCAG ACGGCACGTG ATGAACGAGC TGCTGGAGAC AGAGAGAGCG
    TATGTGGAGG AGCTGCTGTG CGTGTTGGAG GGTTATGCAG CAGAAATGGA CAATCCTGCC
    ATGTCTCACC TCATCCCCAA CACACTGCTT CACAAGAAGG ACATCCTCTT CGGAAACATG
    TCTGAGATCT ACCAGTTCCA CAAGAAGACT TTTCTTCGTG AACTGGAGGC TTACACAGAT
    TATCCTGAGC TTGTGGGACA GTGTTTCTTG GAAAGAATGA CAGATCTGCA GATTTATGAG
    AAGTACTGTC AGAACAAACC TCGCTCAGAG TCTTTATGGA GACAGTGCTC TGACTGCGCT
    TTCTTCCAGG AGTGTCAAAA GAAACTGGAA CATAAGCTTG GTCTGGACTC ATACCTGCTG
    AAACCTGTGC AGAGAATAAC CAAATACCAG CTTTTACTAA AGGAGCTGCT GAAGTACAGT
    AAAGGCTGTG AGGGTGAGGA GGACCTACAG GAGGCGCTCT CATCCATTCT TGGCATCCTA
    AAAGCTGTGA ACGACTCTAT GCACCTCATC GCCATCACTG GATACGAAGG TAACCTCAGT
    GATCTGGGCC GTCTGATGAT GCAGGGCTCC TTCAGCGTGT GGACGGAGCA TAAGAAGGGT
    CACGCCAAGG TAAAAGACCT GGCACGATTC AAGCCCATGC AGAGACACCT GTTCCTGCAC
    GAGAAAGCTC TGCTCTTCTG TAAGAAACGA GAGGAGAACG GGGAGGGTTA TGAGAAAGCC
    CCGTCATACA GCTTCAAACA CTCGCTCAGT ATGAGTGCTG TGGGTATTAC AGAGAACGCC
    AAAGGAGACA ACAAAAAGTT TGAGATCTGG TGTAATTCTC GAGAGGAAGT GTATATTGTG
    CAGGCATCAA CACCAGAGAT CAAAACAGCA TGGGTGAATG AGATCCGGAA GGTTCTGACA
    AGGCAGCTAA AGGCCTGCAG GGAGGCGAGC CAGCAGAAGA GCTCCGATCA GATCTCCCCA
    TTAACTCCCA GCAGCACCGG CCTGACCCTG AGCCCCTTCA AAACAAACCC AAAGAGCCTG
    AAGAAAGGAG ACGAGAAGAA AACAGAACCC ACGACCACAG ATCCCAGCTC AGCCGCCTTA
    TTCAAAAACA ACGACAAGCT CAAAGACGAG ACTGTCGTCA GTCCCAACAC TGAGAGAGCT
    GCAGTGGCTA AAAAGCGCTT TACACTGCAG GGCTTCAGTA ACCTCAAGAG TCAAAAAGGA
    TCATCCCCCC CTAGTCCCGA CCACAAAACT AAAAGACAGA GTGATCCTAC ACCGTTTGGC
    TTCAGAGACG CAGGTCCTCA CATCACTCTC ACCCGAGTGA GATGGCTGAG CACTTCTAGT
    CTGTTGCAGA CTAAACGCAG AGGCTTCACC AAGGCGTCGC TGTCTATGGA TGCGTCTGAA
    GAAAACGACG GATACTCGAG CGCCGAGGAC CCCATGAACT CTGACCCTGA GGATGAGGGA
    GGGAAGAAAT TGTCTCCAGG CAAGTACGTA GTGGTTGCTG ACCATGACAA AAGTGGACCA
    CAGGAGCTGA CTGTTAAGAG CGGAGACACT GTGCAACTGG TCCGAGAGGG AGACGATGGA
    CGCTGGTTTG TTCGTAACCT GCGGACCAGC AAAGAGGGCT GGGTTCCTGC TGCAAACCTG
    CTCACGCTCA TCGGAGAGTC CAAATCTTCT CAGTCTCTCT CCAGCTCAGA AGGCAGTGGG
    TCTGGAAACC TCAGTACATC GTCCAGCTGC AGTGAAACCT ACACAAGCTT CTCGGACATT
    AAAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332808.1
    CDS16..3564
    Translation

    Target ORF information:

    RefSeq Version XM_021477133.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTCTCCT TGTTCTCATG CACCAGGAAA CACAGTCCTA GTAGAGATGA GATTATGCAG 
    CAGGAGAGCA GTCCTCTGTG TGCCGCAGAC ATCAGCCCAG ACCTCAGGAA ACAGTTTGCC
    TTCTTGTCTG GTGGCCGAGG ACAGAACGGC AGCCCCATCA TTATTTTCCC AGAGTACCCG
    GAGTTTGGTG AGCTGGAGGA GCAGGAGTTT CACAATGTGC TGACATACCT GACCAGCATT
    CCCAGTCTCA GCTCGACGGG GATCGGCTTC ATCCTGGTCA TTGACCGCCG GCAAGACAGA
    TGGGCATCAG TGAAGGGGAC ACTGCTGCGG ATCGCGGGCT CTTTCCCAGG CAACCTGCAG
    CTGGTGCTGG TACTGAGACC CACAACACTC TTCCAGCGCA CAATCTCAGA CATATTATTT
    AAATTCAACA AAGATGACTT TAAAATGAAG GTGCCGGTGA TCATGCTCAG CTCTGTGACT
    GAGCTCCACA GTTACATCGA CCGCTCTCAG CTCACTCAGG AACTGGGCGG CACGCAGGAA
    TACTGCCACG AGAAGTGGAT TTCACACCGC ACCGCCATTG AGGGTTTTGC TCTGATGGTG
    AAGAAAACAG CACAGACTTT GCAGTCTTTC GGGACGGAGC TGGCGGAGAC TGAACTGCCT
    AATGATGTTG AGGCCACGTC CAACCTTCTT ACCGTCCACA CCGAGAAGAA GGACAAAATG
    AAGGAGGACC TCAGGATCGC TCTCTGTCAG GGAGGACGTT TGCTGGAGAG CATCAATGAG
    CCGCTGGTGA AAGATCCAGA TTATAGCATG AACCACGATG AACTGGAGAA CCTGGCTACT
    GTTCAGAGGT TGCTCGGTCA GCTAGATGAG ACTGAAACGG CATTTGATGA TTTCTGGGAT
    AAACATCAGA CCAAGCTGGA GCAATGTCTG CAACTGCGGC ACTTTGAGCA GAACTTCCGG
    GAGGTTAGAG CTCACCTAGA CCTGGTTTAC GACCGACTAT CAGGATTCTC CGAAGTAGGA
    ATCAGCCCGG CCCATGCTGA ACACATCCTC AAAGAACTGA CCAATCACGA AGAGAGAGCA
    TCCGAGGTGA TGGACCAGGC TCTGTCTCTT GCGAGTGATG CAGATCAGCT GATTGAAGCG
    TCTCATTATG CTGTGGACTC CATCCTGCCC AAATGCTCTG AGCTGCGCTC TGTGTGTGAG
    GAGATCAGCA CCATCCTGAG GGCCAAGAAA GCCTATTTGT TCAAATCCGT GGAGCTTCAC
    CAGTGTCTGG AGAAGGCCAC TAAATGGTAT GATGATGGAA TCTACCTGCT GGCGTCTCAG
    CCTGTGGATA AGTGTCAGTC ACAGGATGGG GCGGAGTCAG CGCTGCAAGA GATTGAACGT
    TACTTGGATA CAGCCAATCA GCACAAGCTA ACAGATTTGA ATGGAATTTG GAGGGACTAT
    GATTCCGTAC TCACACAAGA CTTCAGGGAT CAGGTTGACA AAGTGTTTCA GAAGCAGCTG
    TCCATGCAGG AAATGTTTGA GAAGAGAAGG GTCAGTCTGA AGAAGCTAGC AGCTAAACAA
    ACTCGACCCG TGCAGCCTGT CGCTCCACGT CCTGAAGCCA TCATCAAAAC ACCAGTCTCA
    TCTCCAGCTC ATAGAGAGAA GTCTATAGAA GGCGACGTCA TCAATGGAAA CTGCAAAAAA
    GGAGAGCTGC ATTTACAGAA CGAGGGCAGC AGCAGACACC CGTCCGTCTC TGATGAAGAG
    GAGAACTTCG CTGTGCTCAG ACGGCACGTG ATGAACGAGC TGCTGGAGAC AGAGAGAGCG
    TATGTGGAGG AGCTGCTGTG CGTGTTGGAG GGTTATGCAG CAGAAATGGA CAATCCTGCC
    ATGTCTCACC TCATCCCCAA CACACTGCTT CACAAGAAGG ACATCCTCTT CGGAAACATG
    TCTGAGATCT ACCAGTTCCA CAAGAAGACT TTTCTTCGTG AACTGGAGGC TTACACAGAT
    TATCCTGAGC TTGTGGGACA GTGTTTCTTG GAAAGAATGA CAGATCTGCA GATTTATGAG
    AAGTACTGTC AGAACAAACC TCGCTCAGAG TCTTTATGGA GACAGTGCTC TGACTGCGCT
    TTCTTCCAGG AGTGTCAAAA GAAACTGGAA CATAAGCTTG GTCTGGACTC ATACCTGCTG
    AAACCTGTGC AGAGAATAAC CAAATACCAG CTTTTACTAA AGGAGCTGCT GAAGTACAGT
    AAAGGCTGTG AGGGTGAGGA GGACCTACAG GAGGCGCTCT CATCCATTCT TGGCATCCTA
    AAAGCTGTGA ACGACTCTAT GCACCTCATC GCCATCACTG GATACGAAGG TAACCTCAGT
    GATCTGGGCC GTCTGATGAT GCAGGGCTCC TTCAGCGTGT GGACGGAGCA TAAGAAGGGT
    CACGCCAAGG TAAAAGACCT GGCACGATTC AAGCCCATGC AGAGACACCT GTTCCTGCAC
    GAGAAAGCTC TGCTCTTCTG TAAGAAACGA GAGGAGAACG GGGAGGGTTA TGAGAAAGCC
    CCGTCATACA GCTTCAAACA CTCGCTCAGT ATGAGTGCTG TGGGTATTAC AGAGAACGCC
    AAAGGAGACA ACAAAAAGTT TGAGATCTGG TGTAATTCTC GAGAGGAAGT GTATATTGTG
    CAGGCATCAA CACCAGAGAT CAAAACAGCA TGGGTGAATG AGATCCGGAA GGTTCTGACA
    AGGCAGCTAA AGGCCTGCAG GGAGGCGAGC CAGCAGAAGA GCTCCGATCA GATCTCCCCA
    TTAACTCCCA GCAGCACCGG CCTGACCCTG AGCCCCTTCA AAACAAACCC AAAGAGCCTG
    AAGAAAGGAG ACGAGAAGAA AACAGAACCC ACGACCACAG ATCCCAGCTC AGCCGCCTTA
    TTCAAAAACA ACGACAAGCT CAAAGACGAG ACTGTCGTCA GTCCCAACAC TGAGAGAGCT
    GCAGTGGCTA AAAAGCGCTT TACACTGCAG GGCTTCAGTA ACCTCAAGAG TCAAAAAGGA
    TCATCCCCCC CTAGTCCCGA CCACAAAACT AAAAGACAGA GTGATCCTAC ACCGTTTGGC
    TTCAGAGACG CAGGTCCTCA CATCACTCTC ACCCGAGTGA GATGGCTGAG CACTTCTAGT
    CTGTTGCAGA CTAAACGCAG AGGCTTCACC AAGGCGTCGC TGTCTATGGA TGCGTCTGAA
    GAAAACGACG GATACTCGAG CGCCGAGGAC CCCATGAACT CTGACCCTGA GGATGAGGGA
    GGGAAGAAAT TGTCTCCAGG CAAGTACGTA GTGGTTGCTG ACCATGACAA AAGTGGACCA
    CAGGAGCTGA CTGTTAAGAG CGGAGACACT GTGCAACTGG TCCGAGAGGG AGACGATGGA
    CGCTGGTTTG TTCGTAACCT GCGGACCAGC AAAGAGGGCT GGGTTCCTGC TGCAAACCTG
    CTCACGCTCA TCGGAGAGTC CAAATCTTCT CAGTCTCTCT CCAGCTCAGA AGGCAGTGGG
    TCTGGAAACC TCAGTACATC GTCCAGCTGC AGTGAAACCT ACACAAGCTT CTCGGACATT
    AAAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147445
    Clone ID Related Accession (Same CDS sequence) XM_021477145.1
    Accession Version XM_021477145.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGGTGC CGGTGATCAT GCTCAGCTCT GTGACTGAGC TCCACAGTTA CATCGACCGC 
    TCTCAGCTCA CTCAGGAACT GGGCGGCACG CAGGAATACT GCCACGAGAA GTGGATTTCA
    CACCGCACCG CCATTGAGGG TTTTGCTCTG ATGGTGAAGA AAACAGCACA GACTTTGCAG
    TCTTTCGGGA CGGAGCTGGC GGAGACTGAA CTGCCTAATG ATGTTGAGGC CACGTCCAAC
    CTTCTTACCG TCCACACCGA GAAGAAGGAC AAAATGAAGG AGGACCTCAG GATCGCTCTC
    TGTCAGGGAG GACGTTTGCT GGAGAGCATC AATGAGCCGC TGGTGAAAGA TCCAGATTAT
    AGCATGAACC ACGATGAACT GGAGAACCTG GCTACTGTTC AGAGGTTGCT CGGTCAGCTA
    GATGAGACTG AAACGGCATT TGATGATTTC TGGGATAAAC ATCAGACCAA GCTGGAGCAA
    TGTCTGCAAC TGCGGCACTT TGAGCAGAAC TTCCGGGAGG TTAGAGCTCA CCTAGACCTG
    GTTTACGACC GACTATCAGG ATTCTCCGAA GTAGGAATCA GCCCGGCCCA TGCTGAACAC
    ATCCTCAAAG AACTGACCAA TCACGAAGAG AGAGCATCCG AGGTGATGGA CCAGGCTCTG
    TCTCTTGCGA GTGATGCAGA TCAGCTGATT GAAGCGTCTC ATTATGCTGT GGACTCCATC
    CTGCCCAAAT GCTCTGAGCT GCGCTCTGTG TGTGAGGAGA TCAGCACCAT CCTGAGGGCC
    AAGAAAGCCT ATTTGTTCAA ATCCGTGGAG CTTCACCAGT GTCTGGAGAA GGCCACTAAA
    TGGTATGATG ATGGAATCTA CCTGCTGGCG TCTCAGCCTG TGGATAAGTG TCAGTCACAG
    GATGGGGCGG AGTCAGCGCT GCAAGAGATT GAACGTTACT TGGATACAGC CAATCAGCAC
    AAGCTAACAG ATTTGAATGG AATTTGGAGG GACTATGATT CCGTACTCAC ACAAGACTTC
    AGGGATCAGG TTGACAAAGT GTTTCAGAAG CAGCTGTCCA TGCAGGAAAT GTTTGAGAAG
    AGAAGGGTCA GTCTGAAGAA GCTAGCAGCT AAACAAACTC GACCCGTGCA GCCTGTCGCT
    CCACGTCCTG AAGCCATCAT CAAAACACCA GTCTCATCTC CAGCTCATAG AGAGAAGTCT
    ATAGAAGGCG ACGTCATCAA TGGAAACTGC AAAAAAGGAG AGCTGCATTT ACAGAACGAG
    GGCAGCAGCA GACACCCGTC CGTCTCTGAT GAAGAGGAGA ACTTCGCTGT GCTCAGACGG
    CACGTGATGA ACGAGCTGCT GGAGACAGAG AGAGCGTATG TGGAGGAGCT GCTGTGCGTG
    TTGGAGGGTT ATGCAGCAGA AATGGACAAT CCTGCCATGT CTCACCTCAT CCCCAACACA
    CTGCTTCACA AGAAGGACAT CCTCTTCGGA AACATGTCTG AGATCTACCA GTTCCACAAG
    AAGACTTTTC TTCGTGAACT GGAGGCTTAC ACAGATTATC CTGAGCTTGT GGGACAGTGT
    TTCTTGGAAA GAATGACAGA TCTGCAGATT TATGAGAAGT ACTGTCAGAA CAAACCTCGC
    TCAGAGTCTT TATGGAGACA GTGCTCTGAC TGCGCTTTCT TCCAGGAGTG TCAAAAGAAA
    CTGGAACATA AGCTTGGTCT GGACTCATAC CTGCTGAAAC CTGTGCAGAG AATAACCAAA
    TACCAGCTTT TACTAAAGGA GCTGCTGAAG TACAGTAAAG GCTGTGAGGG TGAGGAGGAC
    CTACAGGAGG CGCTCTCATC CATTCTTGGC ATCCTAAAAG CTGTGAACGA CTCTATGCAC
    CTCATCGCCA TCACTGGATA CGAAGGTAAC CTCAGTGATC TGGGCCGTCT GATGATGCAG
    GGCTCCTTCA GCGTGTGGAC GGAGCATAAG AAGGGTCACG CCAAGGTAAA AGACCTGGCA
    CGATTCAAGC CCATGCAGAG ACACCTGTTC CTGCACGAGA AAGCTCTGCT CTTCTGTAAG
    AAACGAGAGG AGAACGGGGA GGGTTATGAG AAAGCCCCGT CATACAGCTT CAAACACTCG
    CTCAGTATGA GTGCTGTGGG TATTACAGAG AACGCCAAAG GAGACAACAA AAAGTTTGAG
    ATCTGGTGTA ATTCTCGAGA GGAAGTGTAT ATTGTGCAGG CATCAACACC AGAGATCAAA
    ACAGCATGGG TGAATGAGAT CCGGAAGGTT CTGACAAGGC AGCTAAAGGC CTGCAGGGAG
    GCGAGCCAGC AGAAGAGCTC CGATCAGATC TCCCCATTAA CTCCCAGCAG CACCGGCCTG
    ACCCTGAGCC CCTTCAAAAC AAACCCAAAG AGCCTGAAGA AAGGAGACGA GAAGAAAACA
    GAACCCACGA CCACAGATCC CAGCTCAGCC GCCTTATTCA AAAACAACGA CAAGCTCAAA
    GACGAGACTG TCGTCAGTCC CAACACTGAG AGAGCTGCAG TGGCTAAAAA GCGCTTTACA
    CTGCAGGGCT TCAGTAACCT CAAGAGTCAA AAAGGATCAT CCCCCCCTAG TCCCGACCAC
    AAAACTAAAA GACAGAGTGA TCCTACACCG TTTGGCTTCA GAGACGCAGG TCCTCACATC
    ACTCTCACCC GAGTGAGATG GCTGAGCACT TCTAGTCTGT TGCAGACTAA ACGCAGAGGC
    TTCACCAAGG CGTCGCTGTC TATGGATGCG TCTGAAGAAA ACGACGGATA CTCGAGCGCC
    GAGGACCCCA TGAACTCTGA CCCTGAGGAT GAGGGAGGGA AGAAATTGTC TCCAGGCAAG
    TACGTAGTGG TTGCTGACCA TGACAAAAGT GGACCACAGG AGCTGACTGT TAAGAGCGGA
    GACACTGTGC AACTGGTCCG AGAGGGAGAC GATGGACGCT GGTTTGTTCG TAACCTGCGG
    ACCAGCAAAG AGGGCTGGGT TCCTGCTGCA AACCTGCTCA CGCTCATCGG AGAGTCCAAA
    TCTTCTCAGT CTCTCTCCAG CTCAGAAGGC AGTGGGTCTG GAAACCTCAG TACATCGTCC
    AGCTGCAGTG AAACCTACAC AAGCTTCTCG GACATTAAAG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332820.1
    CDS527..3631
    Translation

    Target ORF information:

    RefSeq Version XM_021477145.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477145.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGGTGC CGGTGATCAT GCTCAGCTCT GTGACTGAGC TCCACAGTTA CATCGACCGC 
    TCTCAGCTCA CTCAGGAACT GGGCGGCACG CAGGAATACT GCCACGAGAA GTGGATTTCA
    CACCGCACCG CCATTGAGGG TTTTGCTCTG ATGGTGAAGA AAACAGCACA GACTTTGCAG
    TCTTTCGGGA CGGAGCTGGC GGAGACTGAA CTGCCTAATG ATGTTGAGGC CACGTCCAAC
    CTTCTTACCG TCCACACCGA GAAGAAGGAC AAAATGAAGG AGGACCTCAG GATCGCTCTC
    TGTCAGGGAG GACGTTTGCT GGAGAGCATC AATGAGCCGC TGGTGAAAGA TCCAGATTAT
    AGCATGAACC ACGATGAACT GGAGAACCTG GCTACTGTTC AGAGGTTGCT CGGTCAGCTA
    GATGAGACTG AAACGGCATT TGATGATTTC TGGGATAAAC ATCAGACCAA GCTGGAGCAA
    TGTCTGCAAC TGCGGCACTT TGAGCAGAAC TTCCGGGAGG TTAGAGCTCA CCTAGACCTG
    GTTTACGACC GACTATCAGG ATTCTCCGAA GTAGGAATCA GCCCGGCCCA TGCTGAACAC
    ATCCTCAAAG AACTGACCAA TCACGAAGAG AGAGCATCCG AGGTGATGGA CCAGGCTCTG
    TCTCTTGCGA GTGATGCAGA TCAGCTGATT GAAGCGTCTC ATTATGCTGT GGACTCCATC
    CTGCCCAAAT GCTCTGAGCT GCGCTCTGTG TGTGAGGAGA TCAGCACCAT CCTGAGGGCC
    AAGAAAGCCT ATTTGTTCAA ATCCGTGGAG CTTCACCAGT GTCTGGAGAA GGCCACTAAA
    TGGTATGATG ATGGAATCTA CCTGCTGGCG TCTCAGCCTG TGGATAAGTG TCAGTCACAG
    GATGGGGCGG AGTCAGCGCT GCAAGAGATT GAACGTTACT TGGATACAGC CAATCAGCAC
    AAGCTAACAG ATTTGAATGG AATTTGGAGG GACTATGATT CCGTACTCAC ACAAGACTTC
    AGGGATCAGG TTGACAAAGT GTTTCAGAAG CAGCTGTCCA TGCAGGAAAT GTTTGAGAAG
    AGAAGGGTCA GTCTGAAGAA GCTAGCAGCT AAACAAACTC GACCCGTGCA GCCTGTCGCT
    CCACGTCCTG AAGCCATCAT CAAAACACCA GTCTCATCTC CAGCTCATAG AGAGAAGTCT
    ATAGAAGGCG ACGTCATCAA TGGAAACTGC AAAAAAGGAG AGCTGCATTT ACAGAACGAG
    GGCAGCAGCA GACACCCGTC CGTCTCTGAT GAAGAGGAGA ACTTCGCTGT GCTCAGACGG
    CACGTGATGA ACGAGCTGCT GGAGACAGAG AGAGCGTATG TGGAGGAGCT GCTGTGCGTG
    TTGGAGGGTT ATGCAGCAGA AATGGACAAT CCTGCCATGT CTCACCTCAT CCCCAACACA
    CTGCTTCACA AGAAGGACAT CCTCTTCGGA AACATGTCTG AGATCTACCA GTTCCACAAG
    AAGACTTTTC TTCGTGAACT GGAGGCTTAC ACAGATTATC CTGAGCTTGT GGGACAGTGT
    TTCTTGGAAA GAATGACAGA TCTGCAGATT TATGAGAAGT ACTGTCAGAA CAAACCTCGC
    TCAGAGTCTT TATGGAGACA GTGCTCTGAC TGCGCTTTCT TCCAGGAGTG TCAAAAGAAA
    CTGGAACATA AGCTTGGTCT GGACTCATAC CTGCTGAAAC CTGTGCAGAG AATAACCAAA
    TACCAGCTTT TACTAAAGGA GCTGCTGAAG TACAGTAAAG GCTGTGAGGG TGAGGAGGAC
    CTACAGGAGG CGCTCTCATC CATTCTTGGC ATCCTAAAAG CTGTGAACGA CTCTATGCAC
    CTCATCGCCA TCACTGGATA CGAAGGTAAC CTCAGTGATC TGGGCCGTCT GATGATGCAG
    GGCTCCTTCA GCGTGTGGAC GGAGCATAAG AAGGGTCACG CCAAGGTAAA AGACCTGGCA
    CGATTCAAGC CCATGCAGAG ACACCTGTTC CTGCACGAGA AAGCTCTGCT CTTCTGTAAG
    AAACGAGAGG AGAACGGGGA GGGTTATGAG AAAGCCCCGT CATACAGCTT CAAACACTCG
    CTCAGTATGA GTGCTGTGGG TATTACAGAG AACGCCAAAG GAGACAACAA AAAGTTTGAG
    ATCTGGTGTA ATTCTCGAGA GGAAGTGTAT ATTGTGCAGG CATCAACACC AGAGATCAAA
    ACAGCATGGG TGAATGAGAT CCGGAAGGTT CTGACAAGGC AGCTAAAGGC CTGCAGGGAG
    GCGAGCCAGC AGAAGAGCTC CGATCAGATC TCCCCATTAA CTCCCAGCAG CACCGGCCTG
    ACCCTGAGCC CCTTCAAAAC AAACCCAAAG AGCCTGAAGA AAGGAGACGA GAAGAAAACA
    GAACCCACGA CCACAGATCC CAGCTCAGCC GCCTTATTCA AAAACAACGA CAAGCTCAAA
    GACGAGACTG TCGTCAGTCC CAACACTGAG AGAGCTGCAG TGGCTAAAAA GCGCTTTACA
    CTGCAGGGCT TCAGTAACCT CAAGAGTCAA AAAGGATCAT CCCCCCCTAG TCCCGACCAC
    AAAACTAAAA GACAGAGTGA TCCTACACCG TTTGGCTTCA GAGACGCAGG TCCTCACATC
    ACTCTCACCC GAGTGAGATG GCTGAGCACT TCTAGTCTGT TGCAGACTAA ACGCAGAGGC
    TTCACCAAGG CGTCGCTGTC TATGGATGCG TCTGAAGAAA ACGACGGATA CTCGAGCGCC
    GAGGACCCCA TGAACTCTGA CCCTGAGGAT GAGGGAGGGA AGAAATTGTC TCCAGGCAAG
    TACGTAGTGG TTGCTGACCA TGACAAAAGT GGACCACAGG AGCTGACTGT TAAGAGCGGA
    GACACTGTGC AACTGGTCCG AGAGGGAGAC GATGGACGCT GGTTTGTTCG TAACCTGCGG
    ACCAGCAAAG AGGGCTGGGT TCCTGCTGCA AACCTGCTCA CGCTCATCGG AGAGTCCAAA
    TCTTCTCAGT CTCTCTCCAG CTCAGAAGGC AGTGGGTCTG GAAACCTCAG TACATCGTCC
    AGCTGCAGTG AAACCTACAC AAGCTTCTCG GACATTAAAG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53916
    Clone ID Related Accession (Same CDS sequence) XM_017356467.1
    Accession Version XM_017356467.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATTCTGA TGGAGGACAC GGGGTCGCAC CAGCAGCCCC CGGAGAACCT GGTGAAGATC 
    CCTTTAGAGG AGATGGAAAG TTTCTACCGG TTCCTTGTTT CCTCTGATGG GCTGATCTAT
    GAGATTATGC AGCAGGAGAG CAGTCCTCTG TGTGCCGCAG ACATCAGCCC AGACCTCAGG
    AAACAGTTTG CCTTCTTGTC TGGTGGCCGA GGACAGAACG GCAGCCCCAT CATTATTTTC
    CCAGAGTACC CGGAGTTTGG TGAGCTGGAG GAGCAGGAGT TTCACAATGT GCTGACATAC
    CTGACCAGCA TTCCCAGTCT CAGCTCGACG GGGATCGGCT TCATCCTGGT CATTGACCGC
    CGGCAAGACA GATGGGCATC AGTGAAGGGG ACACTGCTGC GGATCGCGGG CTCTTTCCCA
    GGCAACCTGC AGCTGGTGCT GGTACTGAGA CCCACAACAC TCTTCCAGCG CACAATCTCA
    GACATATTAT TTAAATTCAA CAAAGATGAC TTTAAAATGA AGGTGCCGGT GATCATGCTC
    AGCTCTGTGA CTGAGCTCCA CAGTTACATC GACCGCTCTC AGCTCACTCA GGAACTGGGC
    GGCACGCAGG AATACTGCCA CGAGAAGTGG ATTTCACACC GCACCGCCAT TGAGGGTTTT
    GCTCTGATGG TGAAGAAAAC AGCACAGACT TTGCAGTCTT TCGGGACGGA GCTGGCGGAG
    ACTGAACTGC CTAATGATGT TGAGGCCACG TCCAACCTTC TTACCGTCCA CACCGAGAAG
    AAGGACAAAA TGAAGGAGGA CCTCAGGATC GCTCTCTGTC AGGGAGGACG TTTGCTGGAG
    AGCATCAATG AGCCGCTGGT GAAAGATCCA GATTATAGCA TGAACCACGA TGAACTGGAG
    AACCTGGCTA CTGTTCAGAG GTTGCTCGGT CAGCTAGATG AGACTGAAAC GGCATTTGAT
    GATTTCTGGG ATAAACATCA GACCAAGCTG GAGCAATGTC TGCAACTGCG GCACTTTGAG
    CAGAACTTCC GGGAGGTTAG AGCTCACCTA GACCTGGTTT ACGACCGACT ATCAGGATTC
    TCCGAAGTAG GAATCAGCCC GGCCCATGCT GAACACATCC TCAAAGAACT GACCAATCAC
    GAAGAGAGAG CATCCGAGGT GATGGACCAG GCTCTGTCTC TTGCGAGTGA TGCAGATCAG
    CTGATTGAAG CGTCTCATTA TGCTGTGGAC TCCATCCTGC CCAAATGCTC TGAGCTGCGC
    TCTGTGTGTG AGGAGATCAG CACCATCCTG AGGGCCAAGA AAGCCTATTT GTTCAAATCC
    GTGGAGCTTC ACCAGTGTCT GGAGAAGGCC ACTAAATGGT ATGATGATGG AATCTACCTG
    CTGGCGTCTC AGCCTGTGGA TAAGTGTCAG TCACAGGATG GGGCGGAGTC AGCGCTGCAA
    GAGATTGAAC GTTACTTGGA TACAGCCAAT CAGCACAAGC TAACAGATTT GAATGGAATT
    TGGAGGGACT ATGATTCCGT ACTCACACAA GACTTCAGGG ATCAGGTTGA CAAAGTGTTT
    CAGAAGCAGC TGTCCATGCA GGAAATGTTT GAGAAGAGAA GGGTCAGTCT GAAGAAGCTA
    GCAGCTAAAC AAACTCGACC CGTGCAGCCT GTCGCTCCAC GTCCTGAAGC CATCATCAAA
    ACACCAGTCT CATCTCCAGC TCATAGAGAG AAGTCTATAG AAGGCGACGT CATCAATGGA
    AACTGCAAAA AAGGAGAGCT GCATTTACAG AACGAGGGCA GCAGCAGACA CCCGTCCGTC
    TCTGATGAAG AGGAGAACTT CGCTGTGCTC AGACGGCACG TGATGAACGA GCTGCTGGAG
    ACAGAGAGAG CGTATGTGGA GGAGCTGCTG TGCGTGTTGG AGGGTTATGC AGCAGAAATG
    GACAATCCTG CCATGTCTCA CCTCATCCCC AACACACTGC TTCACAAGAA GGACATCCTC
    TTCGGAAACA TGTCTGAGAT CTACCAGTTC CACAAGAAGA CTTTTCTTCG TGAACTGGAG
    GCTTACACAG ATTATCCTGA GCTTGTGGGA CAGTGTTTCT TGGAAAGAAT GACAGATCTG
    CAGATTTATG AGAAGTACTG TCAGAACAAA CCTCGCTCAG AGTCTTTATG GAGACAGTGC
    TCTGACTGCG CTTTCTTCCA GGAGTGTCAA AAGAAACTGG AACATAAGCT TGGTCTGGAC
    TCATACCTGC TGAAACCTGT GCAGAGAATA ACCAAATACC AGCTTTTACT AAAGGAGCTG
    CTGAAGTACA GTAAAGGCTG TGAGGGTGAG GAGGACCTAC AGGAGGCGCT CTCATCCATT
    CTTGGCATCC TAAAAGCTGT GAACGACTCT ATGCACCTCA TCGCCATCAC TGGATACGAA
    GGTAACCTCA GTGATCTGGG CCGTCTGATG ATGCAGGGCT CCTTCAGCGT GTGGACGGAG
    CATAAGAAGG GTCACGCCAA GGTAAAAGAC CTGGCACGAT TCAAGCCCAT GCAGAGACAC
    CTGTTCCTGC ACGAGAAAGC TCTGCTCTTC TGTAAGAAAC GAGAGGAGAA CGGGGAGGGT
    TATGAGAAAG CCCCGTCATA CAGCTTCAAA CACTCGCTCA GTATGAGTGC TGTGGGTATT
    ACAGAGAACG CCAAAGGAGA CAACAAAAAG TTTGAGATCT GGTGTAATTC TCGAGAGGAA
    GTGTATATTG TGCAGGCATC AACACCAGAG ATCAAAACAG CATGGGTGAA TGAGATCCGG
    AAGGTTCTGA CAAGGCAGCT AAAGGCCTGC AGGGAGGCGA GCCAGCAGAA GAGCTCCGAT
    CAGATCTCCC CATTAACTCC CAGCAGCACC GGCCTGACCC TGAGCCCCTT CAAAACAAAC
    CCAAAGAGCC TGAAGAAAGG AGACGAGAAG AAAACAGAAC CCACGACCAC AGATCCCAGC
    TCAGCCGCCT TATTCAAAAA CAACGACAAG CTCAAAGACG AGACTGTCGT CAGTCCCAAC
    ACTGAGAGAG CTGCAGTGGC TAAAAAGCGC TTTACACTGC AGGGCTTCAG TAACCTCAAG
    AGTCAAAAAG AGCTGCTAGC GGCCTCTGGT GGTAAAAGTC TAACACTGCC CATGGTCACG
    CTCTGTCATC CAGGATCATC CCCCCCTAGT CCCGACCACA AAACTAAAAG ACAGAGTGAT
    CCTACACCGT TTGGCTTCAG AGGCTTCACC AAGGCGTCGC TGTCTATGGA TGCGTCTGAA
    GAAAACGACG GATACTCGAG CGCCGAGGAC CCCATGAACT CTGACCCTGA GGATGAGGGA
    GGGAAGAAAT TGTCTCCAGG CAAGTACGTA GTGGTTGCTG ACCATGACAA AAGTGGACCA
    CAGGAGCTGA CTGTTAAGAG CGGAGACACT GTGCAACTGG TCCGAGAGGG AGACGATGGA
    CGCTGGTTTG TTCGTAACCT GCGGACCAGC AAAGAGGGCT GGGTTCCTGC TGCAAACCTG
    CTCACGCTCA TCGGAGAGTC CAAATCTTCT CAGTCTCTCT CCAGCTCAGA AGGCAGTGGG
    TCTGGAAACC TCAGTACATC GTCCAGCTGC AGTGAAACCT ACACAAGCTT CTCGGACATT
    AAAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211956.1
    CDS86..3694
    Translation

    Target ORF information:

    RefSeq Version XM_017356467.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356467.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATTCTGA TGGAGGACAC GGGGTCGCAC CAGCAGCCCC CGGAGAACCT GGTGAAGATC 
    CCTTTAGAGG AGATGGAAAG TTTCTACCGG TTCCTTGTTT CCTCTGATGG GCTGATCTAT
    GAGATTATGC AGCAGGAGAG CAGTCCTCTG TGTGCCGCAG ACATCAGCCC AGACCTCAGG
    AAACAGTTTG CCTTCTTGTC TGGTGGCCGA GGACAGAACG GCAGCCCCAT CATTATTTTC
    CCAGAGTACC CGGAGTTTGG TGAGCTGGAG GAGCAGGAGT TTCACAATGT GCTGACATAC
    CTGACCAGCA TTCCCAGTCT CAGCTCGACG GGGATCGGCT TCATCCTGGT CATTGACCGC
    CGGCAAGACA GATGGGCATC AGTGAAGGGG ACACTGCTGC GGATCGCGGG CTCTTTCCCA
    GGCAACCTGC AGCTGGTGCT GGTACTGAGA CCCACAACAC TCTTCCAGCG CACAATCTCA
    GACATATTAT TTAAATTCAA CAAAGATGAC TTTAAAATGA AGGTGCCGGT GATCATGCTC
    AGCTCTGTGA CTGAGCTCCA CAGTTACATC GACCGCTCTC AGCTCACTCA GGAACTGGGC
    GGCACGCAGG AATACTGCCA CGAGAAGTGG ATTTCACACC GCACCGCCAT TGAGGGTTTT
    GCTCTGATGG TGAAGAAAAC AGCACAGACT TTGCAGTCTT TCGGGACGGA GCTGGCGGAG
    ACTGAACTGC CTAATGATGT TGAGGCCACG TCCAACCTTC TTACCGTCCA CACCGAGAAG
    AAGGACAAAA TGAAGGAGGA CCTCAGGATC GCTCTCTGTC AGGGAGGACG TTTGCTGGAG
    AGCATCAATG AGCCGCTGGT GAAAGATCCA GATTATAGCA TGAACCACGA TGAACTGGAG
    AACCTGGCTA CTGTTCAGAG GTTGCTCGGT CAGCTAGATG AGACTGAAAC GGCATTTGAT
    GATTTCTGGG ATAAACATCA GACCAAGCTG GAGCAATGTC TGCAACTGCG GCACTTTGAG
    CAGAACTTCC GGGAGGTTAG AGCTCACCTA GACCTGGTTT ACGACCGACT ATCAGGATTC
    TCCGAAGTAG GAATCAGCCC GGCCCATGCT GAACACATCC TCAAAGAACT GACCAATCAC
    GAAGAGAGAG CATCCGAGGT GATGGACCAG GCTCTGTCTC TTGCGAGTGA TGCAGATCAG
    CTGATTGAAG CGTCTCATTA TGCTGTGGAC TCCATCCTGC CCAAATGCTC TGAGCTGCGC
    TCTGTGTGTG AGGAGATCAG CACCATCCTG AGGGCCAAGA AAGCCTATTT GTTCAAATCC
    GTGGAGCTTC ACCAGTGTCT GGAGAAGGCC ACTAAATGGT ATGATGATGG AATCTACCTG
    CTGGCGTCTC AGCCTGTGGA TAAGTGTCAG TCACAGGATG GGGCGGAGTC AGCGCTGCAA
    GAGATTGAAC GTTACTTGGA TACAGCCAAT CAGCACAAGC TAACAGATTT GAATGGAATT
    TGGAGGGACT ATGATTCCGT ACTCACACAA GACTTCAGGG ATCAGGTTGA CAAAGTGTTT
    CAGAAGCAGC TGTCCATGCA GGAAATGTTT GAGAAGAGAA GGGTCAGTCT GAAGAAGCTA
    GCAGCTAAAC AAACTCGACC CGTGCAGCCT GTCGCTCCAC GTCCTGAAGC CATCATCAAA
    ACACCAGTCT CATCTCCAGC TCATAGAGAG AAGTCTATAG AAGGCGACGT CATCAATGGA
    AACTGCAAAA AAGGAGAGCT GCATTTACAG AACGAGGGCA GCAGCAGACA CCCGTCCGTC
    TCTGATGAAG AGGAGAACTT CGCTGTGCTC AGACGGCACG TGATGAACGA GCTGCTGGAG
    ACAGAGAGAG CGTATGTGGA GGAGCTGCTG TGCGTGTTGG AGGGTTATGC AGCAGAAATG
    GACAATCCTG CCATGTCTCA CCTCATCCCC AACACACTGC TTCACAAGAA GGACATCCTC
    TTCGGAAACA TGTCTGAGAT CTACCAGTTC CACAAGAAGA CTTTTCTTCG TGAACTGGAG
    GCTTACACAG ATTATCCTGA GCTTGTGGGA CAGTGTTTCT TGGAAAGAAT GACAGATCTG
    CAGATTTATG AGAAGTACTG TCAGAACAAA CCTCGCTCAG AGTCTTTATG GAGACAGTGC
    TCTGACTGCG CTTTCTTCCA GGAGTGTCAA AAGAAACTGG AACATAAGCT TGGTCTGGAC
    TCATACCTGC TGAAACCTGT GCAGAGAATA ACCAAATACC AGCTTTTACT AAAGGAGCTG
    CTGAAGTACA GTAAAGGCTG TGAGGGTGAG GAGGACCTAC AGGAGGCGCT CTCATCCATT
    CTTGGCATCC TAAAAGCTGT GAACGACTCT ATGCACCTCA TCGCCATCAC TGGATACGAA
    GGTAACCTCA GTGATCTGGG CCGTCTGATG ATGCAGGGCT CCTTCAGCGT GTGGACGGAG
    CATAAGAAGG GTCACGCCAA GGTAAAAGAC CTGGCACGAT TCAAGCCCAT GCAGAGACAC
    CTGTTCCTGC ACGAGAAAGC TCTGCTCTTC TGTAAGAAAC GAGAGGAGAA CGGGGAGGGT
    TATGAGAAAG CCCCGTCATA CAGCTTCAAA CACTCGCTCA GTATGAGTGC TGTGGGTATT
    ACAGAGAACG CCAAAGGAGA CAACAAAAAG TTTGAGATCT GGTGTAATTC TCGAGAGGAA
    GTGTATATTG TGCAGGCATC AACACCAGAG ATCAAAACAG CATGGGTGAA TGAGATCCGG
    AAGGTTCTGA CAAGGCAGCT AAAGGCCTGC AGGGAGGCGA GCCAGCAGAA GAGCTCCGAT
    CAGATCTCCC CATTAACTCC CAGCAGCACC GGCCTGACCC TGAGCCCCTT CAAAACAAAC
    CCAAAGAGCC TGAAGAAAGG AGACGAGAAG AAAACAGAAC CCACGACCAC AGATCCCAGC
    TCAGCCGCCT TATTCAAAAA CAACGACAAG CTCAAAGACG AGACTGTCGT CAGTCCCAAC
    ACTGAGAGAG CTGCAGTGGC TAAAAAGCGC TTTACACTGC AGGGCTTCAG TAACCTCAAG
    AGTCAAAAAG AGCTGCTAGC GGCCTCTGGT GGTAAAAGTC TAACACTGCC CATGGTCACG
    CTCTGTCATC CAGGATCATC CCCCCCTAGT CCCGACCACA AAACTAAAAG ACAGAGTGAT
    CCTACACCGT TTGGCTTCAG AGGCTTCACC AAGGCGTCGC TGTCTATGGA TGCGTCTGAA
    GAAAACGACG GATACTCGAG CGCCGAGGAC CCCATGAACT CTGACCCTGA GGATGAGGGA
    GGGAAGAAAT TGTCTCCAGG CAAGTACGTA GTGGTTGCTG ACCATGACAA AAGTGGACCA
    CAGGAGCTGA CTGTTAAGAG CGGAGACACT GTGCAACTGG TCCGAGAGGG AGACGATGGA
    CGCTGGTTTG TTCGTAACCT GCGGACCAGC AAAGAGGGCT GGGTTCCTGC TGCAAACCTG
    CTCACGCTCA TCGGAGAGTC CAAATCTTCT CAGTCTCTCT CCAGCTCAGA AGGCAGTGGG
    TCTGGAAACC TCAGTACATC GTCCAGCTGC AGTGAAACCT ACACAAGCTT CTCGGACATT
    AAAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53917
    Clone ID Related Accession (Same CDS sequence) XM_017356469.1
    Accession Version XM_017356469.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGCTCA ACCGAGTGTC CCTGCTCTGT CAGGACATTA CCAAACTCTG GCTGCAGCTC 
    AAAGTCTTGA CAGATGAGAT TATGCAGCAG GAGAGCAGTC CTCTGTGTGC CGCAGACATC
    AGCCCAGACC TCAGGAAACA GTTTGCCTTC TTGTCTGGTG GCCGAGGACA GAACGGCAGC
    CCCATCATTA TTTTCCCAGA GTACCCGGAG TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC
    AATGTGCTGA CATACCTGAC CAGCATTCCC AGTCTCAGCT CGACGGGGAT CGGCTTCATC
    CTGGTCATTG ACCGCCGGCA AGACAGATGG GCATCAGTGA AGGGGACACT GCTGCGGATC
    GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG GTGCTGGTAC TGAGACCCAC AACACTCTTC
    CAGCGCACAA TCTCAGACAT ATTATTTAAA TTCAACAAAG ATGACTTTAA AATGAAGGTG
    CCGGTGATCA TGCTCAGCTC TGTGACTGAG CTCCACAGTT ACATCGACCG CTCTCAGCTC
    ACTCAGGAAC TGGGCGGCAC GCAGGAATAC TGCCACGAGA AGTGGATTTC ACACCGCACC
    GCCATTGAGG GTTTTGCTCT GATGGTGAAG AAAACAGCAC AGACTTTGCA GTCTTTCGGG
    ACGGAGCTGG CGGAGACTGA ACTGCCTAAT GATGTTGAGG CCACGTCCAA CCTTCTTACC
    GTCCACACCG AGAAGAAGGA CAAAATGAAG GAGGACCTCA GGATCGCTCT CTGTCAGGGA
    GGACGTTTGC TGGAGAGCAT CAATGAGCCG CTGGTGAAAG ATCCAGATTA TAGCATGAAC
    CACGATGAAC TGGAGAACCT GGCTACTGTT CAGAGGTTGC TCGGTCAGCT AGATGAGACT
    GAAACGGCAT TTGATGATTT CTGGGATAAA CATCAGACCA AGCTGGAGCA ATGTCTGCAA
    CTGCGGCACT TTGAGCAGAA CTTCCGGGAG GTTAGAGCTC ACCTAGACCT GGTTTACGAC
    CGACTATCAG GATTCTCCGA AGTAGGAATC AGCCCGGCCC ATGCTGAACA CATCCTCAAA
    GAACTGACCA ATCACGAAGA GAGAGCATCC GAGGTGATGG ACCAGGCTCT GTCTCTTGCG
    AGTGATGCAG ATCAGCTGAT TGAAGCGTCT CATTATGCTG TGGACTCCAT CCTGCCCAAA
    TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG ATCAGCACCA TCCTGAGGGC CAAGAAAGCC
    TATTTGTTCA AATCCGTGGA GCTTCACCAG TGTCTGGAGA AGGCCACTAA ATGGTATGAT
    GATGGAATCT ACCTGCTGGC GTCTCAGCCT GTGGATAAGT GTCAGTCACA GGATGGGGCG
    GAGTCAGCGC TGCAAGAGAT TGAACGTTAC TTGGATACAG CCAATCAGCA CAAGCTAACA
    GATTTGAATG GAATTTGGAG GGACTATGAT TCCGTACTCA CACAAGACTT CAGGGATCAG
    GTTGACAAAG TGTTTCAGAA GCAGCTGTCC ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC
    AGTCTGAAGA AGCTAGCAGC TAAACAAACT CGACCCGTGC AGCCTGTCGC TCCACGTCCT
    GAAGCCATCA TCAAAACACC AGTCTCATCT CCAGCTCATA GAGAGAAGTC TATAGAAGGC
    GACGTCATCA ATGGAAACTG CAAAAAAGGA GAGCTGCATT TACAGAACGA GGGCAGCAGC
    AGACACCCGT CCGTCTCTGA TGAAGAGGAG AACTTCGCTG TGCTCAGACG GCACGTGATG
    AACGAGCTGC TGGAGACAGA GAGAGCGTAT GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT
    TATGCAGCAG AAATGGACAA TCCTGCCATG TCTCACCTCA TCCCCAACAC ACTGCTTCAC
    AAGAAGGACA TCCTCTTCGG AAACATGTCT GAGATCTACC AGTTCCACAA GAAGACTTTT
    CTTCGTGAAC TGGAGGCTTA CACAGATTAT CCTGAGCTTG TGGGACAGTG TTTCTTGGAA
    AGAATGACAG ATCTGCAGAT TTATGAGAAG TACTGTCAGA ACAAACCTCG CTCAGAGTCT
    TTATGGAGAC AGTGCTCTGA CTGCGCTTTC TTCCAGGAGT GTCAAAAGAA ACTGGAACAT
    AAGCTTGGTC TGGACTCATA CCTGCTGAAA CCTGTGCAGA GAATAACCAA ATACCAGCTT
    TTACTAAAGG AGCTGCTGAA GTACAGTAAA GGCTGTGAGG GTGAGGAGGA CCTACAGGAG
    GCGCTCTCAT CCATTCTTGG CATCCTAAAA GCTGTGAACG ACTCTATGCA CCTCATCGCC
    ATCACTGGAT ACGAAGGTAA CCTCAGTGAT CTGGGCCGTC TGATGATGCA GGGCTCCTTC
    AGCGTGTGGA CGGAGCATAA GAAGGGTCAC GCCAAGGTAA AAGACCTGGC ACGATTCAAG
    CCCATGCAGA GACACCTGTT CCTGCACGAG AAAGCTCTGC TCTTCTGTAA GAAACGAGAG
    GAGAACGGGG AGGGTTATGA GAAAGCCCCG TCATACAGCT TCAAACACTC GCTCAGTATG
    AGTGCTGTGG GTATTACAGA GAACGCCAAA GGAGACAACA AAAAGTTTGA GATCTGGTGT
    AATTCTCGAG AGGAAGTGTA TATTGTGCAG GCATCAACAC CAGAGATCAA AACAGCATGG
    GTGAATGAGA TCCGGAAGGT TCTGACAAGG CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG
    CAGAAGAGCT CCGATCAGAT CTCCCCATTA ACTCCCAGCA GCACCGGCCT GACCCTGAGC
    CCCTTCAAAA CAAACCCAAA GAGCCTGAAG AAAGGAGACG AGAAGAAAAC AGAACCCACG
    ACCACAGATC CCAGCTCAGC CGCCTTATTC AAAAACAACG ACAAGCTCAA AGACGAGACT
    GTCGTCAGTC CCAACACTGA GAGAGCTGCA GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC
    TTCAGTAACC TCAAGAGTCA AAAAGAGCTG CTAGCGGCCT CTGGTGGTAA AAGTCTAACA
    CTGCCCATGG TCACGCTCTG TCATCCAGGA TCATCCCCCC CTAGTCCCGA CCACAAAACT
    AAAAGACAGA GTGATCCTAC ACCGTTTGGC TTCAGAGGCT TCACCAAGGC GTCGCTGTCT
    ATGGATGCGT CTGAAGAAAA CGACGGATAC TCGAGCGCCG AGGACCCCAT GAACTCTGAC
    CCTGAGGATG AGGGAGGGAA GAAATTGTCT CCAGGCAAGT ACGTAGTGGT TGCTGACCAT
    GACAAAAGTG GACCACAGGA GCTGACTGTT AAGAGCGGAG ACACTGTGCA ACTGGTCCGA
    GAGGGAGACG ATGGACGCTG GTTTGTTCGT AACCTGCGGA CCAGCAAAGA GGGCTGGGTT
    CCTGCTGCAA ACCTGCTCAC GCTCATCGGA GAGTCCAAAT CTTCTCAGTC TCTCTCCAGC
    TCAGAAGGCA GTGGGTCTGG AAACCTCAGT ACATCGTCCA GCTGCAGTGA AACCTACACA
    AGCTTCTCGG ACATTAAAGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211958.1
    CDS370..3933
    Translation

    Target ORF information:

    RefSeq Version XM_017356469.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356469.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGCTCA ACCGAGTGTC CCTGCTCTGT CAGGACATTA CCAAACTCTG GCTGCAGCTC 
    AAAGTCTTGA CAGATGAGAT TATGCAGCAG GAGAGCAGTC CTCTGTGTGC CGCAGACATC
    AGCCCAGACC TCAGGAAACA GTTTGCCTTC TTGTCTGGTG GCCGAGGACA GAACGGCAGC
    CCCATCATTA TTTTCCCAGA GTACCCGGAG TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC
    AATGTGCTGA CATACCTGAC CAGCATTCCC AGTCTCAGCT CGACGGGGAT CGGCTTCATC
    CTGGTCATTG ACCGCCGGCA AGACAGATGG GCATCAGTGA AGGGGACACT GCTGCGGATC
    GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG GTGCTGGTAC TGAGACCCAC AACACTCTTC
    CAGCGCACAA TCTCAGACAT ATTATTTAAA TTCAACAAAG ATGACTTTAA AATGAAGGTG
    CCGGTGATCA TGCTCAGCTC TGTGACTGAG CTCCACAGTT ACATCGACCG CTCTCAGCTC
    ACTCAGGAAC TGGGCGGCAC GCAGGAATAC TGCCACGAGA AGTGGATTTC ACACCGCACC
    GCCATTGAGG GTTTTGCTCT GATGGTGAAG AAAACAGCAC AGACTTTGCA GTCTTTCGGG
    ACGGAGCTGG CGGAGACTGA ACTGCCTAAT GATGTTGAGG CCACGTCCAA CCTTCTTACC
    GTCCACACCG AGAAGAAGGA CAAAATGAAG GAGGACCTCA GGATCGCTCT CTGTCAGGGA
    GGACGTTTGC TGGAGAGCAT CAATGAGCCG CTGGTGAAAG ATCCAGATTA TAGCATGAAC
    CACGATGAAC TGGAGAACCT GGCTACTGTT CAGAGGTTGC TCGGTCAGCT AGATGAGACT
    GAAACGGCAT TTGATGATTT CTGGGATAAA CATCAGACCA AGCTGGAGCA ATGTCTGCAA
    CTGCGGCACT TTGAGCAGAA CTTCCGGGAG GTTAGAGCTC ACCTAGACCT GGTTTACGAC
    CGACTATCAG GATTCTCCGA AGTAGGAATC AGCCCGGCCC ATGCTGAACA CATCCTCAAA
    GAACTGACCA ATCACGAAGA GAGAGCATCC GAGGTGATGG ACCAGGCTCT GTCTCTTGCG
    AGTGATGCAG ATCAGCTGAT TGAAGCGTCT CATTATGCTG TGGACTCCAT CCTGCCCAAA
    TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG ATCAGCACCA TCCTGAGGGC CAAGAAAGCC
    TATTTGTTCA AATCCGTGGA GCTTCACCAG TGTCTGGAGA AGGCCACTAA ATGGTATGAT
    GATGGAATCT ACCTGCTGGC GTCTCAGCCT GTGGATAAGT GTCAGTCACA GGATGGGGCG
    GAGTCAGCGC TGCAAGAGAT TGAACGTTAC TTGGATACAG CCAATCAGCA CAAGCTAACA
    GATTTGAATG GAATTTGGAG GGACTATGAT TCCGTACTCA CACAAGACTT CAGGGATCAG
    GTTGACAAAG TGTTTCAGAA GCAGCTGTCC ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC
    AGTCTGAAGA AGCTAGCAGC TAAACAAACT CGACCCGTGC AGCCTGTCGC TCCACGTCCT
    GAAGCCATCA TCAAAACACC AGTCTCATCT CCAGCTCATA GAGAGAAGTC TATAGAAGGC
    GACGTCATCA ATGGAAACTG CAAAAAAGGA GAGCTGCATT TACAGAACGA GGGCAGCAGC
    AGACACCCGT CCGTCTCTGA TGAAGAGGAG AACTTCGCTG TGCTCAGACG GCACGTGATG
    AACGAGCTGC TGGAGACAGA GAGAGCGTAT GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT
    TATGCAGCAG AAATGGACAA TCCTGCCATG TCTCACCTCA TCCCCAACAC ACTGCTTCAC
    AAGAAGGACA TCCTCTTCGG AAACATGTCT GAGATCTACC AGTTCCACAA GAAGACTTTT
    CTTCGTGAAC TGGAGGCTTA CACAGATTAT CCTGAGCTTG TGGGACAGTG TTTCTTGGAA
    AGAATGACAG ATCTGCAGAT TTATGAGAAG TACTGTCAGA ACAAACCTCG CTCAGAGTCT
    TTATGGAGAC AGTGCTCTGA CTGCGCTTTC TTCCAGGAGT GTCAAAAGAA ACTGGAACAT
    AAGCTTGGTC TGGACTCATA CCTGCTGAAA CCTGTGCAGA GAATAACCAA ATACCAGCTT
    TTACTAAAGG AGCTGCTGAA GTACAGTAAA GGCTGTGAGG GTGAGGAGGA CCTACAGGAG
    GCGCTCTCAT CCATTCTTGG CATCCTAAAA GCTGTGAACG ACTCTATGCA CCTCATCGCC
    ATCACTGGAT ACGAAGGTAA CCTCAGTGAT CTGGGCCGTC TGATGATGCA GGGCTCCTTC
    AGCGTGTGGA CGGAGCATAA GAAGGGTCAC GCCAAGGTAA AAGACCTGGC ACGATTCAAG
    CCCATGCAGA GACACCTGTT CCTGCACGAG AAAGCTCTGC TCTTCTGTAA GAAACGAGAG
    GAGAACGGGG AGGGTTATGA GAAAGCCCCG TCATACAGCT TCAAACACTC GCTCAGTATG
    AGTGCTGTGG GTATTACAGA GAACGCCAAA GGAGACAACA AAAAGTTTGA GATCTGGTGT
    AATTCTCGAG AGGAAGTGTA TATTGTGCAG GCATCAACAC CAGAGATCAA AACAGCATGG
    GTGAATGAGA TCCGGAAGGT TCTGACAAGG CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG
    CAGAAGAGCT CCGATCAGAT CTCCCCATTA ACTCCCAGCA GCACCGGCCT GACCCTGAGC
    CCCTTCAAAA CAAACCCAAA GAGCCTGAAG AAAGGAGACG AGAAGAAAAC AGAACCCACG
    ACCACAGATC CCAGCTCAGC CGCCTTATTC AAAAACAACG ACAAGCTCAA AGACGAGACT
    GTCGTCAGTC CCAACACTGA GAGAGCTGCA GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC
    TTCAGTAACC TCAAGAGTCA AAAAGAGCTG CTAGCGGCCT CTGGTGGTAA AAGTCTAACA
    CTGCCCATGG TCACGCTCTG TCATCCAGGA TCATCCCCCC CTAGTCCCGA CCACAAAACT
    AAAAGACAGA GTGATCCTAC ACCGTTTGGC TTCAGAGGCT TCACCAAGGC GTCGCTGTCT
    ATGGATGCGT CTGAAGAAAA CGACGGATAC TCGAGCGCCG AGGACCCCAT GAACTCTGAC
    CCTGAGGATG AGGGAGGGAA GAAATTGTCT CCAGGCAAGT ACGTAGTGGT TGCTGACCAT
    GACAAAAGTG GACCACAGGA GCTGACTGTT AAGAGCGGAG ACACTGTGCA ACTGGTCCGA
    GAGGGAGACG ATGGACGCTG GTTTGTTCGT AACCTGCGGA CCAGCAAAGA GGGCTGGGTT
    CCTGCTGCAA ACCTGCTCAC GCTCATCGGA GAGTCCAAAT CTTCTCAGTC TCTCTCCAGC
    TCAGAAGGCA GTGGGTCTGG AAACCTCAGT ACATCGTCCA GCTGCAGTGA AACCTACACA
    AGCTTCTCGG ACATTAAAGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53918
    Clone ID Related Accession (Same CDS sequence) XM_017356470.1
    Accession Version XM_017356470.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAGCAGG AGAGCAGTCC TCTGTGTGCC GCAGACATCA GCCCAGACCT CAGGAAACAG 
    TTTGCCTTCT TGTCTGGTGG CCGAGGACAG AACGGCAGCC CCATCATTAT TTTCCCAGAG
    TACCCGGAGT TTGGTGAGCT GGAGGAGCAG GAGTTTCACA ATGTGCTGAC ATACCTGACC
    AGCATTCCCA GTCTCAGCTC GACGGGGATC GGCTTCATCC TGGTCATTGA CCGCCGGCAA
    GACAGATGGG CATCAGTGAA GGGGACACTG CTGCGGATCG CGGGCTCTTT CCCAGGCAAC
    CTGCAGCTGG TGCTGGTACT GAGACCCACA ACACTCTTCC AGCGCACAAT CTCAGACATA
    TTATTTAAAT TCAACAAAGA TGACTTTAAA ATGAAGGTGC CGGTGATCAT GCTCAGCTCT
    GTGACTGAGC TCCACAGTTA CATCGACCGC TCTCAGCTCA CTCAGGAACT GGGCGGCACG
    CAGGAATACT GCCACGAGAA GTGGATTTCA CACCGCACCG CCATTGAGGG TTTTGCTCTG
    ATGGTGAAGA AAACAGCACA GACTTTGCAG TCTTTCGGGA CGGAGCTGGC GGAGACTGAA
    CTGCCTAATG ATGTTGAGGC CACGTCCAAC CTTCTTACCG TCCACACCGA GAAGAAGGAC
    AAAATGAAGG AGGACCTCAG GATCGCTCTC TGTCAGGGAG GACGTTTGCT GGAGAGCATC
    AATGAGCCGC TGGTGAAAGA TCCAGATTAT AGCATGAACC ACGATGAACT GGAGAACCTG
    GCTACTGTTC AGAGGTTGCT CGGTCAGCTA GATGAGACTG AAACGGCATT TGATGATTTC
    TGGGATAAAC ATCAGACCAA GCTGGAGCAA TGTCTGCAAC TGCGGCACTT TGAGCAGAAC
    TTCCGGGAGG TTAGAGCTCA CCTAGACCTG GTTTACGACC GACTATCAGG ATTCTCCGAA
    GTAGGAATCA GCCCGGCCCA TGCTGAACAC ATCCTCAAAG AACTGACCAA TCACGAAGAG
    AGAGCATCCG AGGTGATGGA CCAGGCTCTG TCTCTTGCGA GTGATGCAGA TCAGCTGATT
    GAAGCGTCTC ATTATGCTGT GGACTCCATC CTGCCCAAAT GCTCTGAGCT GCGCTCTGTG
    TGTGAGGAGA TCAGCACCAT CCTGAGGGCC AAGAAAGCCT ATTTGTTCAA ATCCGTGGAG
    CTTCACCAGT GTCTGGAGAA GGCCACTAAA TGGTATGATG ATGGAATCTA CCTGCTGGCG
    TCTCAGCCTG TGGATAAGTG TCAGTCACAG GATGGGGCGG AGTCAGCGCT GCAAGAGATT
    GAACGTTACT TGGATACAGC CAATCAGCAC AAGCTAACAG ATTTGAATGG AATTTGGAGG
    GACTATGATT CCGTACTCAC ACAAGACTTC AGGGATCAGG TTGACAAAGT GTTTCAGAAG
    CAGCTGTCCA TGCAGGAAAT GTTTGAGAAG AGAAGGGTCA GTCTGAAGAA GCTAGCAGCT
    AAACAAACTC GACCCGTGCA GCCTGTCGCT CCACGTCCTG AAGCCATCAT CAAAACACCA
    GTCTCATCTC CAGCTCATAG AGAGAAGTCT ATAGAAGGCG ACGTCATCAA TGGAAACTGC
    AAAAAAGGAG AGCTGCATTT ACAGAACGAG GGCAGCAGCA GACACCCGTC CGTCTCTGAT
    GAAGAGGAGA ACTTCGCTGT GCTCAGACGG CACGTGATGA ACGAGCTGCT GGAGACAGAG
    AGAGCGTATG TGGAGGAGCT GCTGTGCGTG TTGGAGGGTT ATGCAGCAGA AATGGACAAT
    CCTGCCATGT CTCACCTCAT CCCCAACACA CTGCTTCACA AGAAGGACAT CCTCTTCGGA
    AACATGTCTG AGATCTACCA GTTCCACAAG AAGACTTTTC TTCGTGAACT GGAGGCTTAC
    ACAGATTATC CTGAGCTTGT GGGACAGTGT TTCTTGGAAA GAATGACAGA TCTGCAGATT
    TATGAGAAGT ACTGTCAGAA CAAACCTCGC TCAGAGTCTT TATGGAGACA GTGCTCTGAC
    TGCGCTTTCT TCCAGGAGTG TCAAAAGAAA CTGGAACATA AGCTTGGTCT GGACTCATAC
    CTGCTGAAAC CTGTGCAGAG AATAACCAAA TACCAGCTTT TACTAAAGGA GCTGCTGAAG
    TACAGTAAAG GCTGTGAGGG TGAGGAGGAC CTACAGGAGG CGCTCTCATC CATTCTTGGC
    ATCCTAAAAG CTGTGAACGA CTCTATGCAC CTCATCGCCA TCACTGGATA CGAAGGTAAC
    CTCAGTGATC TGGGCCGTCT GATGATGCAG GGCTCCTTCA GCGTGTGGAC GGAGCATAAG
    AAGGGTCACG CCAAGGTAAA AGACCTGGCA CGATTCAAGC CCATGCAGAG ACACCTGTTC
    CTGCACGAGA AAGCTCTGCT CTTCTGTAAG AAACGAGAGG AGAACGGGGA GGGTTATGAG
    AAAGCCCCGT CATACAGCTT CAAACACTCG CTCAGTATGA GTGCTGTGGG TATTACAGAG
    AACGCCAAAG GAGACAACAA AAAGTTTGAG ATCTGGTGTA ATTCTCGAGA GGAAGTGTAT
    ATTGTGCAGG CATCAACACC AGAGATCAAA ACAGCATGGG TGAATGAGAT CCGGAAGGTT
    CTGACAAGGC AGCTAAAGGC CTGCAGGGAG GCGAGCCAGC AGAAGAGCTC CGATCAGATC
    TCCCCATTAA CTCCCAGCAG CACCGGCCTG ACCCTGAGCC CCTTCAAAAC AAACCCAAAG
    AGCCTGAAGA AAGGAGACGA GAAGAAAACA GAACCCACGA CCACAGATCC CAGCTCAGCC
    GCCTTATTCA AAAACAACGA CAAGCTCAAA GACGAGACTG TCGTCAGTCC CAACACTGAG
    AGAGCTGCAG TGGCTAAAAA GCGCTTTACA CTGCAGGGCT TCAGTAACCT CAAGAGTCAA
    AAAGAGCTGC TAGCGGCCTC TGGTGGTAAA AGTCTAACAC TGCCCATGGT CACGCTCTGT
    CATCCAGGAT CATCCCCCCC TAGTCCCGAC CACAAAACTA AAAGACAGAG TGATCCTACA
    CCGTTTGGCT TCAGAGGCTT CACCAAGGCG TCGCTGTCTA TGGATGCGTC TGAAGAAAAC
    GACGGATACT CGAGCGCCGA GGACCCCATG AACTCTGACC CTGAGGATGA GGGAGGGAAG
    AAATTGTCTC CAGGCAAGTA CGTAGTGGTT GCTGACCATG ACAAAAGTGG ACCACAGGAG
    CTGACTGTTA AGAGCGGAGA CACTGTGCAA CTGGTCCGAG AGGGAGACGA TGGACGCTGG
    TTTGTTCGTA ACCTGCGGAC CAGCAAAGAG GGCTGGGTTC CTGCTGCAAA CCTGCTCACG
    CTCATCGGAG AGTCCAAATC TTCTCAGTCT CTCTCCAGCT CAGAAGGCAG TGGGTCTGGA
    AACCTCAGTA CATCGTCCAG CTGCAGTGAA ACCTACACAA GCTTCTCGGA CATTAAAGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211959.1
    CDS133..3615
    Translation

    Target ORF information:

    RefSeq Version XM_017356470.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356470.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAGCAGG AGAGCAGTCC TCTGTGTGCC GCAGACATCA GCCCAGACCT CAGGAAACAG 
    TTTGCCTTCT TGTCTGGTGG CCGAGGACAG AACGGCAGCC CCATCATTAT TTTCCCAGAG
    TACCCGGAGT TTGGTGAGCT GGAGGAGCAG GAGTTTCACA ATGTGCTGAC ATACCTGACC
    AGCATTCCCA GTCTCAGCTC GACGGGGATC GGCTTCATCC TGGTCATTGA CCGCCGGCAA
    GACAGATGGG CATCAGTGAA GGGGACACTG CTGCGGATCG CGGGCTCTTT CCCAGGCAAC
    CTGCAGCTGG TGCTGGTACT GAGACCCACA ACACTCTTCC AGCGCACAAT CTCAGACATA
    TTATTTAAAT TCAACAAAGA TGACTTTAAA ATGAAGGTGC CGGTGATCAT GCTCAGCTCT
    GTGACTGAGC TCCACAGTTA CATCGACCGC TCTCAGCTCA CTCAGGAACT GGGCGGCACG
    CAGGAATACT GCCACGAGAA GTGGATTTCA CACCGCACCG CCATTGAGGG TTTTGCTCTG
    ATGGTGAAGA AAACAGCACA GACTTTGCAG TCTTTCGGGA CGGAGCTGGC GGAGACTGAA
    CTGCCTAATG ATGTTGAGGC CACGTCCAAC CTTCTTACCG TCCACACCGA GAAGAAGGAC
    AAAATGAAGG AGGACCTCAG GATCGCTCTC TGTCAGGGAG GACGTTTGCT GGAGAGCATC
    AATGAGCCGC TGGTGAAAGA TCCAGATTAT AGCATGAACC ACGATGAACT GGAGAACCTG
    GCTACTGTTC AGAGGTTGCT CGGTCAGCTA GATGAGACTG AAACGGCATT TGATGATTTC
    TGGGATAAAC ATCAGACCAA GCTGGAGCAA TGTCTGCAAC TGCGGCACTT TGAGCAGAAC
    TTCCGGGAGG TTAGAGCTCA CCTAGACCTG GTTTACGACC GACTATCAGG ATTCTCCGAA
    GTAGGAATCA GCCCGGCCCA TGCTGAACAC ATCCTCAAAG AACTGACCAA TCACGAAGAG
    AGAGCATCCG AGGTGATGGA CCAGGCTCTG TCTCTTGCGA GTGATGCAGA TCAGCTGATT
    GAAGCGTCTC ATTATGCTGT GGACTCCATC CTGCCCAAAT GCTCTGAGCT GCGCTCTGTG
    TGTGAGGAGA TCAGCACCAT CCTGAGGGCC AAGAAAGCCT ATTTGTTCAA ATCCGTGGAG
    CTTCACCAGT GTCTGGAGAA GGCCACTAAA TGGTATGATG ATGGAATCTA CCTGCTGGCG
    TCTCAGCCTG TGGATAAGTG TCAGTCACAG GATGGGGCGG AGTCAGCGCT GCAAGAGATT
    GAACGTTACT TGGATACAGC CAATCAGCAC AAGCTAACAG ATTTGAATGG AATTTGGAGG
    GACTATGATT CCGTACTCAC ACAAGACTTC AGGGATCAGG TTGACAAAGT GTTTCAGAAG
    CAGCTGTCCA TGCAGGAAAT GTTTGAGAAG AGAAGGGTCA GTCTGAAGAA GCTAGCAGCT
    AAACAAACTC GACCCGTGCA GCCTGTCGCT CCACGTCCTG AAGCCATCAT CAAAACACCA
    GTCTCATCTC CAGCTCATAG AGAGAAGTCT ATAGAAGGCG ACGTCATCAA TGGAAACTGC
    AAAAAAGGAG AGCTGCATTT ACAGAACGAG GGCAGCAGCA GACACCCGTC CGTCTCTGAT
    GAAGAGGAGA ACTTCGCTGT GCTCAGACGG CACGTGATGA ACGAGCTGCT GGAGACAGAG
    AGAGCGTATG TGGAGGAGCT GCTGTGCGTG TTGGAGGGTT ATGCAGCAGA AATGGACAAT
    CCTGCCATGT CTCACCTCAT CCCCAACACA CTGCTTCACA AGAAGGACAT CCTCTTCGGA
    AACATGTCTG AGATCTACCA GTTCCACAAG AAGACTTTTC TTCGTGAACT GGAGGCTTAC
    ACAGATTATC CTGAGCTTGT GGGACAGTGT TTCTTGGAAA GAATGACAGA TCTGCAGATT
    TATGAGAAGT ACTGTCAGAA CAAACCTCGC TCAGAGTCTT TATGGAGACA GTGCTCTGAC
    TGCGCTTTCT TCCAGGAGTG TCAAAAGAAA CTGGAACATA AGCTTGGTCT GGACTCATAC
    CTGCTGAAAC CTGTGCAGAG AATAACCAAA TACCAGCTTT TACTAAAGGA GCTGCTGAAG
    TACAGTAAAG GCTGTGAGGG TGAGGAGGAC CTACAGGAGG CGCTCTCATC CATTCTTGGC
    ATCCTAAAAG CTGTGAACGA CTCTATGCAC CTCATCGCCA TCACTGGATA CGAAGGTAAC
    CTCAGTGATC TGGGCCGTCT GATGATGCAG GGCTCCTTCA GCGTGTGGAC GGAGCATAAG
    AAGGGTCACG CCAAGGTAAA AGACCTGGCA CGATTCAAGC CCATGCAGAG ACACCTGTTC
    CTGCACGAGA AAGCTCTGCT CTTCTGTAAG AAACGAGAGG AGAACGGGGA GGGTTATGAG
    AAAGCCCCGT CATACAGCTT CAAACACTCG CTCAGTATGA GTGCTGTGGG TATTACAGAG
    AACGCCAAAG GAGACAACAA AAAGTTTGAG ATCTGGTGTA ATTCTCGAGA GGAAGTGTAT
    ATTGTGCAGG CATCAACACC AGAGATCAAA ACAGCATGGG TGAATGAGAT CCGGAAGGTT
    CTGACAAGGC AGCTAAAGGC CTGCAGGGAG GCGAGCCAGC AGAAGAGCTC CGATCAGATC
    TCCCCATTAA CTCCCAGCAG CACCGGCCTG ACCCTGAGCC CCTTCAAAAC AAACCCAAAG
    AGCCTGAAGA AAGGAGACGA GAAGAAAACA GAACCCACGA CCACAGATCC CAGCTCAGCC
    GCCTTATTCA AAAACAACGA CAAGCTCAAA GACGAGACTG TCGTCAGTCC CAACACTGAG
    AGAGCTGCAG TGGCTAAAAA GCGCTTTACA CTGCAGGGCT TCAGTAACCT CAAGAGTCAA
    AAAGAGCTGC TAGCGGCCTC TGGTGGTAAA AGTCTAACAC TGCCCATGGT CACGCTCTGT
    CATCCAGGAT CATCCCCCCC TAGTCCCGAC CACAAAACTA AAAGACAGAG TGATCCTACA
    CCGTTTGGCT TCAGAGGCTT CACCAAGGCG TCGCTGTCTA TGGATGCGTC TGAAGAAAAC
    GACGGATACT CGAGCGCCGA GGACCCCATG AACTCTGACC CTGAGGATGA GGGAGGGAAG
    AAATTGTCTC CAGGCAAGTA CGTAGTGGTT GCTGACCATG ACAAAAGTGG ACCACAGGAG
    CTGACTGTTA AGAGCGGAGA CACTGTGCAA CTGGTCCGAG AGGGAGACGA TGGACGCTGG
    TTTGTTCGTA ACCTGCGGAC CAGCAAAGAG GGCTGGGTTC CTGCTGCAAA CCTGCTCACG
    CTCATCGGAG AGTCCAAATC TTCTCAGTCT CTCTCCAGCT CAGAAGGCAG TGGGTCTGGA
    AACCTCAGTA CATCGTCCAG CTGCAGTGAA ACCTACACAA GCTTCTCGGA CATTAAAGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53919
    Clone ID Related Accession (Same CDS sequence) XM_017356474.1
    Accession Version XM_017356474.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGGTGC CGGTGATCAT GCTCAGCTCT GTGACTGAGC TCCACAGTTA CATCGACCGC 
    TCTCAGCTCA CTCAGGAACT GGGCGGCACG CAGGAATACT GCCACGAGAA GTGGATTTCA
    CACCGCACCG CCATTGAGGG TTTTGCTCTG ATGGTGAAGA AAACAGCACA GACTTTGCAG
    TCTTTCGGGA CGGAGCTGGC GGAGACTGAA CTGCCTAATG ATGTTGAGGC CACGTCCAAC
    CTTCTTACCG TCCACACCGA GAAGAAGGAC AAAATGAAGG AGGACCTCAG GATCGCTCTC
    TGTCAGGGAG GACGTTTGCT GGAGAGCATC AATGAGCCGC TGGTGAAAGA TCCAGATTAT
    AGCATGAACC ACGATGAACT GGAGAACCTG GCTACTGTTC AGAGGTTGCT CGGTCAGCTA
    GATGAGACTG AAACGGCATT TGATGATTTC TGGGATAAAC ATCAGACCAA GCTGGAGCAA
    TGTCTGCAAC TGCGGCACTT TGAGCAGAAC TTCCGGGAGG TTAGAGCTCA CCTAGACCTG
    GTTTACGACC GACTATCAGG ATTCTCCGAA GTAGGAATCA GCCCGGCCCA TGCTGAACAC
    ATCCTCAAAG AACTGACCAA TCACGAAGAG AGAGCATCCG AGGTGATGGA CCAGGCTCTG
    TCTCTTGCGA GTGATGCAGA TCAGCTGATT GAAGCGTCTC ATTATGCTGT GGACTCCATC
    CTGCCCAAAT GCTCTGAGCT GCGCTCTGTG TGTGAGGAGA TCAGCACCAT CCTGAGGGCC
    AAGAAAGCCT ATTTGTTCAA ATCCGTGGAG CTTCACCAGT GTCTGGAGAA GGCCACTAAA
    TGGTATGATG ATGGAATCTA CCTGCTGGCG TCTCAGCCTG TGGATAAGTG TCAGTCACAG
    GATGGGGCGG AGTCAGCGCT GCAAGAGATT GAACGTTACT TGGATACAGC CAATCAGCAC
    AAGCTAACAG ATTTGAATGG AATTTGGAGG GACTATGATT CCGTACTCAC ACAAGACTTC
    AGGGATCAGG TTGACAAAGT GTTTCAGAAG CAGCTGTCCA TGCAGGAAAT GTTTGAGAAG
    AGAAGGGTCA GTCTGAAGAA GCTAGCAGCT AAACAAACTC GACCCGTGCA GCCTGTCGCT
    CCACGTCCTG AAGCCATCAT CAAAACACCA GTCTCATCTC CAGCTCATAG AGAGAAGTCT
    ATAGAAGGCG ACGTCATCAA TGGAAACTGC AAAAAAGGAG AGCTGCATTT ACAGAACGAG
    GGCAGCAGCA GACACCCGTC CGTCTCTGAT GAAGAGGAGA ACTTCGCTGT GCTCAGACGG
    CACGTGATGA ACGAGCTGCT GGAGACAGAG AGAGCGTATG TGGAGGAGCT GCTGTGCGTG
    TTGGAGGGTT ATGCAGCAGA AATGGACAAT CCTGCCATGT CTCACCTCAT CCCCAACACA
    CTGCTTCACA AGAAGGACAT CCTCTTCGGA AACATGTCTG AGATCTACCA GTTCCACAAG
    AAGACTTTTC TTCGTGAACT GGAGGCTTAC ACAGATTATC CTGAGCTTGT GGGACAGTGT
    TTCTTGGAAA GAATGACAGA TCTGCAGATT TATGAGAAGT ACTGTCAGAA CAAACCTCGC
    TCAGAGTCTT TATGGAGACA GTGCTCTGAC TGCGCTTTCT TCCAGGAGTG TCAAAAGAAA
    CTGGAACATA AGCTTGGTCT GGACTCATAC CTGCTGAAAC CTGTGCAGAG AATAACCAAA
    TACCAGCTTT TACTAAAGGA GCTGCTGAAG TACAGTAAAG GCTGTGAGGG TGAGGAGGAC
    CTACAGGAGG CGCTCTCATC CATTCTTGGC ATCCTAAAAG CTGTGAACGA CTCTATGCAC
    CTCATCGCCA TCACTGGATA CGAAGGTAAC CTCAGTGATC TGGGCCGTCT GATGATGCAG
    GGCTCCTTCA GCGTGTGGAC GGAGCATAAG AAGGGTCACG CCAAGGTAAA AGACCTGGCA
    CGATTCAAGC CCATGCAGAG ACACCTGTTC CTGCACGAGA AAGCTCTGCT CTTCTGTAAG
    AAACGAGAGG AGAACGGGGA GGGTTATGAG AAAGCCCCGT CATACAGCTT CAAACACTCG
    CTCAGTATGA GTGCTGTGGG TATTACAGAG AACGCCAAAG GAGACAACAA AAAGTTTGAG
    ATCTGGTGTA ATTCTCGAGA GGAAGTGTAT ATTGTGCAGG CATCAACACC AGAGATCAAA
    ACAGCATGGG TGAATGAGAT CCGGAAGGTT CTGACAAGGC AGCTAAAGGC CTGCAGGGAG
    GCGAGCCAGC AGAAGAGCTC CGATCAGATC TCCCCATTAA CTCCCAGCAG CACCGGCCTG
    ACCCTGAGCC CCTTCAAAAC AAACCCAAAG AGCCTGAAGA AAGGAGACGA GAAGAAAACA
    GAACCCACGA CCACAGATCC CAGCTCAGCC GCCTTATTCA AAAACAACGA CAAGCTCAAA
    GACGAGACTG TCGTCAGTCC CAACACTGAG AGAGCTGCAG TGGCTAAAAA GCGCTTTACA
    CTGCAGGGCT TCAGTAACCT CAAGAGTCAA AAAGAGCTGC TAGCGGCCTC TGGTGGTAAA
    AGTCTAACAC TGCCCATGGT CACGCTCTGT CATCCAGGAT CATCCCCCCC TAGTCCCGAC
    CACAAAACTA AAAGACAGAG TGATCCTACA CCGTTTGGCT TCAGAGGCTT CACCAAGGCG
    TCGCTGTCTA TGGATGCGTC TGAAGAAAAC GACGGATACT CGAGCGCCGA GGACCCCATG
    AACTCTGACC CTGAGGATGA GGGAGGGAAG AAATTGTCTC CAGGCAAGTA CGTAGTGGTT
    GCTGACCATG ACAAAAGTGG ACCACAGGAG CTGACTGTTA AGAGCGGAGA CACTGTGCAA
    CTGGTCCGAG AGGGAGACGA TGGACGCTGG TTTGTTCGTA ACCTGCGGAC CAGCAAAGAG
    GGCTGGGTTC CTGCTGCAAA CCTGCTCACG CTCATCGGAG AGTCCAAATC TTCTCAGTCT
    CTCTCCAGCT CAGAAGGCAG TGGGTCTGGA AACCTCAGTA CATCGTCCAG CTGCAGTGAA
    ACCTACACAA GCTTCTCGGA CATTAAAGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211963.1
    CDS529..3621
    Translation

    Target ORF information:

    RefSeq Version XM_017356474.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356474.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGGTGC CGGTGATCAT GCTCAGCTCT GTGACTGAGC TCCACAGTTA CATCGACCGC 
    TCTCAGCTCA CTCAGGAACT GGGCGGCACG CAGGAATACT GCCACGAGAA GTGGATTTCA
    CACCGCACCG CCATTGAGGG TTTTGCTCTG ATGGTGAAGA AAACAGCACA GACTTTGCAG
    TCTTTCGGGA CGGAGCTGGC GGAGACTGAA CTGCCTAATG ATGTTGAGGC CACGTCCAAC
    CTTCTTACCG TCCACACCGA GAAGAAGGAC AAAATGAAGG AGGACCTCAG GATCGCTCTC
    TGTCAGGGAG GACGTTTGCT GGAGAGCATC AATGAGCCGC TGGTGAAAGA TCCAGATTAT
    AGCATGAACC ACGATGAACT GGAGAACCTG GCTACTGTTC AGAGGTTGCT CGGTCAGCTA
    GATGAGACTG AAACGGCATT TGATGATTTC TGGGATAAAC ATCAGACCAA GCTGGAGCAA
    TGTCTGCAAC TGCGGCACTT TGAGCAGAAC TTCCGGGAGG TTAGAGCTCA CCTAGACCTG
    GTTTACGACC GACTATCAGG ATTCTCCGAA GTAGGAATCA GCCCGGCCCA TGCTGAACAC
    ATCCTCAAAG AACTGACCAA TCACGAAGAG AGAGCATCCG AGGTGATGGA CCAGGCTCTG
    TCTCTTGCGA GTGATGCAGA TCAGCTGATT GAAGCGTCTC ATTATGCTGT GGACTCCATC
    CTGCCCAAAT GCTCTGAGCT GCGCTCTGTG TGTGAGGAGA TCAGCACCAT CCTGAGGGCC
    AAGAAAGCCT ATTTGTTCAA ATCCGTGGAG CTTCACCAGT GTCTGGAGAA GGCCACTAAA
    TGGTATGATG ATGGAATCTA CCTGCTGGCG TCTCAGCCTG TGGATAAGTG TCAGTCACAG
    GATGGGGCGG AGTCAGCGCT GCAAGAGATT GAACGTTACT TGGATACAGC CAATCAGCAC
    AAGCTAACAG ATTTGAATGG AATTTGGAGG GACTATGATT CCGTACTCAC ACAAGACTTC
    AGGGATCAGG TTGACAAAGT GTTTCAGAAG CAGCTGTCCA TGCAGGAAAT GTTTGAGAAG
    AGAAGGGTCA GTCTGAAGAA GCTAGCAGCT AAACAAACTC GACCCGTGCA GCCTGTCGCT
    CCACGTCCTG AAGCCATCAT CAAAACACCA GTCTCATCTC CAGCTCATAG AGAGAAGTCT
    ATAGAAGGCG ACGTCATCAA TGGAAACTGC AAAAAAGGAG AGCTGCATTT ACAGAACGAG
    GGCAGCAGCA GACACCCGTC CGTCTCTGAT GAAGAGGAGA ACTTCGCTGT GCTCAGACGG
    CACGTGATGA ACGAGCTGCT GGAGACAGAG AGAGCGTATG TGGAGGAGCT GCTGTGCGTG
    TTGGAGGGTT ATGCAGCAGA AATGGACAAT CCTGCCATGT CTCACCTCAT CCCCAACACA
    CTGCTTCACA AGAAGGACAT CCTCTTCGGA AACATGTCTG AGATCTACCA GTTCCACAAG
    AAGACTTTTC TTCGTGAACT GGAGGCTTAC ACAGATTATC CTGAGCTTGT GGGACAGTGT
    TTCTTGGAAA GAATGACAGA TCTGCAGATT TATGAGAAGT ACTGTCAGAA CAAACCTCGC
    TCAGAGTCTT TATGGAGACA GTGCTCTGAC TGCGCTTTCT TCCAGGAGTG TCAAAAGAAA
    CTGGAACATA AGCTTGGTCT GGACTCATAC CTGCTGAAAC CTGTGCAGAG AATAACCAAA
    TACCAGCTTT TACTAAAGGA GCTGCTGAAG TACAGTAAAG GCTGTGAGGG TGAGGAGGAC
    CTACAGGAGG CGCTCTCATC CATTCTTGGC ATCCTAAAAG CTGTGAACGA CTCTATGCAC
    CTCATCGCCA TCACTGGATA CGAAGGTAAC CTCAGTGATC TGGGCCGTCT GATGATGCAG
    GGCTCCTTCA GCGTGTGGAC GGAGCATAAG AAGGGTCACG CCAAGGTAAA AGACCTGGCA
    CGATTCAAGC CCATGCAGAG ACACCTGTTC CTGCACGAGA AAGCTCTGCT CTTCTGTAAG
    AAACGAGAGG AGAACGGGGA GGGTTATGAG AAAGCCCCGT CATACAGCTT CAAACACTCG
    CTCAGTATGA GTGCTGTGGG TATTACAGAG AACGCCAAAG GAGACAACAA AAAGTTTGAG
    ATCTGGTGTA ATTCTCGAGA GGAAGTGTAT ATTGTGCAGG CATCAACACC AGAGATCAAA
    ACAGCATGGG TGAATGAGAT CCGGAAGGTT CTGACAAGGC AGCTAAAGGC CTGCAGGGAG
    GCGAGCCAGC AGAAGAGCTC CGATCAGATC TCCCCATTAA CTCCCAGCAG CACCGGCCTG
    ACCCTGAGCC CCTTCAAAAC AAACCCAAAG AGCCTGAAGA AAGGAGACGA GAAGAAAACA
    GAACCCACGA CCACAGATCC CAGCTCAGCC GCCTTATTCA AAAACAACGA CAAGCTCAAA
    GACGAGACTG TCGTCAGTCC CAACACTGAG AGAGCTGCAG TGGCTAAAAA GCGCTTTACA
    CTGCAGGGCT TCAGTAACCT CAAGAGTCAA AAAGAGCTGC TAGCGGCCTC TGGTGGTAAA
    AGTCTAACAC TGCCCATGGT CACGCTCTGT CATCCAGGAT CATCCCCCCC TAGTCCCGAC
    CACAAAACTA AAAGACAGAG TGATCCTACA CCGTTTGGCT TCAGAGGCTT CACCAAGGCG
    TCGCTGTCTA TGGATGCGTC TGAAGAAAAC GACGGATACT CGAGCGCCGA GGACCCCATG
    AACTCTGACC CTGAGGATGA GGGAGGGAAG AAATTGTCTC CAGGCAAGTA CGTAGTGGTT
    GCTGACCATG ACAAAAGTGG ACCACAGGAG CTGACTGTTA AGAGCGGAGA CACTGTGCAA
    CTGGTCCGAG AGGGAGACGA TGGACGCTGG TTTGTTCGTA ACCTGCGGAC CAGCAAAGAG
    GGCTGGGTTC CTGCTGCAAA CCTGCTCACG CTCATCGGAG AGTCCAAATC TTCTCAGTCT
    CTCTCCAGCT CAGAAGGCAG TGGGTCTGGA AACCTCAGTA CATCGTCCAG CTGCAGTGAA
    ACCTACACAA GCTTCTCGGA CATTAAAGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06135
    Clone ID Related Accession (Same CDS sequence) XM_005171007.4 , XM_005171007.3
    Accession Version XM_005171007.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171007.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGAGACT GTCGTCAGTC CCAACACTGA GAGAGCTGCA
    GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC TTCAGTAACC TCAAGAGTCA AAAAGGATCA
    TCCCCCCCTA GTCCCGACCA CAAAACTAAA AGACAGAGTG ATCCTACACC GTTTGGCTTC
    AGAGGCTTCA CCAAGGCGTC GCTGTCTATG GATGCGTCTG AAGAAAACGA CGGATACTCG
    AGCGCCGAGG ACCCCATGAA CTCTGACCCT GAGGATGAGG GAGGGAAGAA ATTGTCTCCA
    GGCAAGTACG TAGTGGTTGC TGACCATGAC AAAAGTGGAC CACAGGAGCT GACTGTTAAG
    AGCGGAGACA CTGTGCAACT GGTCCGAGAG GGAGACGATG GACGCTGGTT TGTTCGTAAC
    CTGCGGACCA GCAAAGAGGG CTGGGTTCCT GCTGCAAACC TGCTCACGCT CATCGGAGAG
    TCCAAATCTT CTCAGTCTCT CTCCAGCTCA GAAGGCAGTG GGTCTGGAAA CCTCAGTACA
    TCGTCCAGCT GCAGTGAAAC CTACACAAGC TTCTCGGACA TTAAAGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171064.1
    CDS285..3875
    Translation

    Target ORF information:

    RefSeq Version XM_005171007.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171007.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGAGACT GTCGTCAGTC CCAACACTGA GAGAGCTGCA
    GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC TTCAGTAACC TCAAGAGTCA AAAAGGATCA
    TCCCCCCCTA GTCCCGACCA CAAAACTAAA AGACAGAGTG ATCCTACACC GTTTGGCTTC
    AGAGGCTTCA CCAAGGCGTC GCTGTCTATG GATGCGTCTG AAGAAAACGA CGGATACTCG
    AGCGCCGAGG ACCCCATGAA CTCTGACCCT GAGGATGAGG GAGGGAAGAA ATTGTCTCCA
    GGCAAGTACG TAGTGGTTGC TGACCATGAC AAAAGTGGAC CACAGGAGCT GACTGTTAAG
    AGCGGAGACA CTGTGCAACT GGTCCGAGAG GGAGACGATG GACGCTGGTT TGTTCGTAAC
    CTGCGGACCA GCAAAGAGGG CTGGGTTCCT GCTGCAAACC TGCTCACGCT CATCGGAGAG
    TCCAAATCTT CTCAGTCTCT CTCCAGCTCA GAAGGCAGTG GGTCTGGAAA CCTCAGTACA
    TCGTCCAGCT GCAGTGAAAC CTACACAAGC TTCTCGGACA TTAAAGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06135
    Clone ID Related Accession (Same CDS sequence) XM_005171007.4 , XM_005171007.3
    Accession Version XM_005171007.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171007.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGAGACT GTCGTCAGTC CCAACACTGA GAGAGCTGCA
    GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC TTCAGTAACC TCAAGAGTCA AAAAGGATCA
    TCCCCCCCTA GTCCCGACCA CAAAACTAAA AGACAGAGTG ATCCTACACC GTTTGGCTTC
    AGAGGCTTCA CCAAGGCGTC GCTGTCTATG GATGCGTCTG AAGAAAACGA CGGATACTCG
    AGCGCCGAGG ACCCCATGAA CTCTGACCCT GAGGATGAGG GAGGGAAGAA ATTGTCTCCA
    GGCAAGTACG TAGTGGTTGC TGACCATGAC AAAAGTGGAC CACAGGAGCT GACTGTTAAG
    AGCGGAGACA CTGTGCAACT GGTCCGAGAG GGAGACGATG GACGCTGGTT TGTTCGTAAC
    CTGCGGACCA GCAAAGAGGG CTGGGTTCCT GCTGCAAACC TGCTCACGCT CATCGGAGAG
    TCCAAATCTT CTCAGTCTCT CTCCAGCTCA GAAGGCAGTG GGTCTGGAAA CCTCAGTACA
    TCGTCCAGCT GCAGTGAAAC CTACACAAGC TTCTCGGACA TTAAAGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171064.1
    CDS301..3891
    Translation

    Target ORF information:

    RefSeq Version XM_005171007.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171007.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGAGACT GTCGTCAGTC CCAACACTGA GAGAGCTGCA
    GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC TTCAGTAACC TCAAGAGTCA AAAAGGATCA
    TCCCCCCCTA GTCCCGACCA CAAAACTAAA AGACAGAGTG ATCCTACACC GTTTGGCTTC
    AGAGGCTTCA CCAAGGCGTC GCTGTCTATG GATGCGTCTG AAGAAAACGA CGGATACTCG
    AGCGCCGAGG ACCCCATGAA CTCTGACCCT GAGGATGAGG GAGGGAAGAA ATTGTCTCCA
    GGCAAGTACG TAGTGGTTGC TGACCATGAC AAAAGTGGAC CACAGGAGCT GACTGTTAAG
    AGCGGAGACA CTGTGCAACT GGTCCGAGAG GGAGACGATG GACGCTGGTT TGTTCGTAAC
    CTGCGGACCA GCAAAGAGGG CTGGGTTCCT GCTGCAAACC TGCTCACGCT CATCGGAGAG
    TCCAAATCTT CTCAGTCTCT CTCCAGCTCA GAAGGCAGTG GGTCTGGAAA CCTCAGTACA
    TCGTCCAGCT GCAGTGAAAC CTACACAAGC TTCTCGGACA TTAAAGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06137
    Clone ID Related Accession (Same CDS sequence) XM_005171009.3 , XM_005171009.4
    Accession Version XM_005171009.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGCAGGT CCTCACATCA CTCTCACCCG AGTGAGATGG
    CTGAGCACTT CTAGTCTGTT GCAGACTAAA CGCAGAGGCT TCACCAAGGC GTCGCTGTCT
    ATGGATGCGT CTGAAGAAAA CGACGGATAC TCGAGCGCCG AGGACCCCAT GAACTCTGAC
    CCTGAGGATG AGGGAGGGAA GAAATTGTCT CCAGGCAAGT ACGTAGTGGT TGCTGACCAT
    GACAAAAGTG GACCACAGGA GCTGACTGTT AAGAGCGGAG ACACTGTGCA ACTGGTCCGA
    GAGGGAGACG ATGGACGCTG GTTTGTTCGT AACCTGCGGA CCAGCAAAGA GGGCTGGGTT
    CCTGCTGCAA ACCTGCTCAC GCTCATCGGA GAGTCCAAAT CTTCTCAGTC TCTCTCCAGC
    TCAGAAGGCA GTGGGTCTGG AAACCTCAGT ACATCGTCCA GCTGCAGTGA AACCTACACA
    AGCTTCTCGG ACATTAAAGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171066.1
    CDS300..3803
    Translation

    Target ORF information:

    RefSeq Version XM_005171009.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171009.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGCAGGT CCTCACATCA CTCTCACCCG AGTGAGATGG
    CTGAGCACTT CTAGTCTGTT GCAGACTAAA CGCAGAGGCT TCACCAAGGC GTCGCTGTCT
    ATGGATGCGT CTGAAGAAAA CGACGGATAC TCGAGCGCCG AGGACCCCAT GAACTCTGAC
    CCTGAGGATG AGGGAGGGAA GAAATTGTCT CCAGGCAAGT ACGTAGTGGT TGCTGACCAT
    GACAAAAGTG GACCACAGGA GCTGACTGTT AAGAGCGGAG ACACTGTGCA ACTGGTCCGA
    GAGGGAGACG ATGGACGCTG GTTTGTTCGT AACCTGCGGA CCAGCAAAGA GGGCTGGGTT
    CCTGCTGCAA ACCTGCTCAC GCTCATCGGA GAGTCCAAAT CTTCTCAGTC TCTCTCCAGC
    TCAGAAGGCA GTGGGTCTGG AAACCTCAGT ACATCGTCCA GCTGCAGTGA AACCTACACA
    AGCTTCTCGG ACATTAAAGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06137
    Clone ID Related Accession (Same CDS sequence) XM_005171009.3 , XM_005171009.4
    Accession Version XM_005171009.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171009.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGCAGGT CCTCACATCA CTCTCACCCG AGTGAGATGG
    CTGAGCACTT CTAGTCTGTT GCAGACTAAA CGCAGAGGCT TCACCAAGGC GTCGCTGTCT
    ATGGATGCGT CTGAAGAAAA CGACGGATAC TCGAGCGCCG AGGACCCCAT GAACTCTGAC
    CCTGAGGATG AGGGAGGGAA GAAATTGTCT CCAGGCAAGT ACGTAGTGGT TGCTGACCAT
    GACAAAAGTG GACCACAGGA GCTGACTGTT AAGAGCGGAG ACACTGTGCA ACTGGTCCGA
    GAGGGAGACG ATGGACGCTG GTTTGTTCGT AACCTGCGGA CCAGCAAAGA GGGCTGGGTT
    CCTGCTGCAA ACCTGCTCAC GCTCATCGGA GAGTCCAAAT CTTCTCAGTC TCTCTCCAGC
    TCAGAAGGCA GTGGGTCTGG AAACCTCAGT ACATCGTCCA GCTGCAGTGA AACCTACACA
    AGCTTCTCGG ACATTAAAGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171066.1
    CDS286..3789
    Translation

    Target ORF information:

    RefSeq Version XM_005171009.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171009.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGCAGGT CCTCACATCA CTCTCACCCG AGTGAGATGG
    CTGAGCACTT CTAGTCTGTT GCAGACTAAA CGCAGAGGCT TCACCAAGGC GTCGCTGTCT
    ATGGATGCGT CTGAAGAAAA CGACGGATAC TCGAGCGCCG AGGACCCCAT GAACTCTGAC
    CCTGAGGATG AGGGAGGGAA GAAATTGTCT CCAGGCAAGT ACGTAGTGGT TGCTGACCAT
    GACAAAAGTG GACCACAGGA GCTGACTGTT AAGAGCGGAG ACACTGTGCA ACTGGTCCGA
    GAGGGAGACG ATGGACGCTG GTTTGTTCGT AACCTGCGGA CCAGCAAAGA GGGCTGGGTT
    CCTGCTGCAA ACCTGCTCAC GCTCATCGGA GAGTCCAAAT CTTCTCAGTC TCTCTCCAGC
    TCAGAAGGCA GTGGGTCTGG AAACCTCAGT ACATCGTCCA GCTGCAGTGA AACCTACACA
    AGCTTCTCGG ACATTAAAGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06138
    Clone ID Related Accession (Same CDS sequence) XM_001922148.6 , XM_001922148.7
    Accession Version XM_001922148.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922148.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGGCTTCACC AAGGCGTCGC TGTCTATGGA TGCGTCTGAA
    GAAAACGACG GATACTCGAG CGCCGAGGAC CCCATGAACT CTGACCCTGA GGATGAGGGA
    GGGAAGAAAT TGTCTCCAGG CAAGTACGTA GTGGTTGCTG ACCATGACAA AAGTGGACCA
    CAGGAGCTGA CTGTTAAGAG CGGAGACACT GTGCAACTGG TCCGAGAGGG AGACGATGGA
    CGCTGGTTTG TTCGTAACCT GCGGACCAGC AAAGAGGGCT GGGTTCCTGC TGCAAACCTG
    CTCACGCTCA TCGGAGAGTC CAAATCTTCT CAGTCTCTCT CCAGCTCAGA AGGCAGTGGG
    TCTGGAAACC TCAGTACATC GTCCAGCTGC AGTGAAACCT ACACAAGCTT CTCGGACATT
    AAAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001922183.4
    CDS300..3728
    Translation

    Target ORF information:

    RefSeq Version XM_001922148.6
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001922148.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGGCTTCACC AAGGCGTCGC TGTCTATGGA TGCGTCTGAA
    GAAAACGACG GATACTCGAG CGCCGAGGAC CCCATGAACT CTGACCCTGA GGATGAGGGA
    GGGAAGAAAT TGTCTCCAGG CAAGTACGTA GTGGTTGCTG ACCATGACAA AAGTGGACCA
    CAGGAGCTGA CTGTTAAGAG CGGAGACACT GTGCAACTGG TCCGAGAGGG AGACGATGGA
    CGCTGGTTTG TTCGTAACCT GCGGACCAGC AAAGAGGGCT GGGTTCCTGC TGCAAACCTG
    CTCACGCTCA TCGGAGAGTC CAAATCTTCT CAGTCTCTCT CCAGCTCAGA AGGCAGTGGG
    TCTGGAAACC TCAGTACATC GTCCAGCTGC AGTGAAACCT ACACAAGCTT CTCGGACATT
    AAAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06138
    Clone ID Related Accession (Same CDS sequence) XM_001922148.6 , XM_001922148.7
    Accession Version XM_001922148.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922148.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGGCTTCACC AAGGCGTCGC TGTCTATGGA TGCGTCTGAA
    GAAAACGACG GATACTCGAG CGCCGAGGAC CCCATGAACT CTGACCCTGA GGATGAGGGA
    GGGAAGAAAT TGTCTCCAGG CAAGTACGTA GTGGTTGCTG ACCATGACAA AAGTGGACCA
    CAGGAGCTGA CTGTTAAGAG CGGAGACACT GTGCAACTGG TCCGAGAGGG AGACGATGGA
    CGCTGGTTTG TTCGTAACCT GCGGACCAGC AAAGAGGGCT GGGTTCCTGC TGCAAACCTG
    CTCACGCTCA TCGGAGAGTC CAAATCTTCT CAGTCTCTCT CCAGCTCAGA AGGCAGTGGG
    TCTGGAAACC TCAGTACATC GTCCAGCTGC AGTGAAACCT ACACAAGCTT CTCGGACATT
    AAAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001922183.4
    CDS285..3713
    Translation

    Target ORF information:

    RefSeq Version XM_001922148.7
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001922148.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGGCTTCACC AAGGCGTCGC TGTCTATGGA TGCGTCTGAA
    GAAAACGACG GATACTCGAG CGCCGAGGAC CCCATGAACT CTGACCCTGA GGATGAGGGA
    GGGAAGAAAT TGTCTCCAGG CAAGTACGTA GTGGTTGCTG ACCATGACAA AAGTGGACCA
    CAGGAGCTGA CTGTTAAGAG CGGAGACACT GTGCAACTGG TCCGAGAGGG AGACGATGGA
    CGCTGGTTTG TTCGTAACCT GCGGACCAGC AAAGAGGGCT GGGTTCCTGC TGCAAACCTG
    CTCACGCTCA TCGGAGAGTC CAAATCTTCT CAGTCTCTCT CCAGCTCAGA AGGCAGTGGG
    TCTGGAAACC TCAGTACATC GTCCAGCTGC AGTGAAACCT ACACAAGCTT CTCGGACATT
    AAAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48644
    Clone ID Related Accession (Same CDS sequence) XM_009293977.2
    Accession Version XM_009293977.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGAGACT GTCGTCAGTC CCAACACTGA GAGAGCTGCA
    GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC TTCAGTAACC TCAAGAGTCA AAAAGAGCTG
    CTAGCGGCCT CTGGTGGTAA AAGTCTAACA CTGCCCATGG TCACGCTCTG TCATCCAGGA
    TCATCCCCCC CTAGTCCCGA CCACAAAACT AAAAGACAGA GTGATCCTAC ACCGTTTGGC
    TTCAGAGGCT TCACCAAGGC GTCGCTGTCT ATGGATGCGT CTGAAGAAAA CGACGGATAC
    TCGAGCGCCG AGGACCCCAT GAACTCTGAC CCTGAGGATG AGGGAGGGAA GAAATTGTCT
    CCAGGCAAGT ACGTAGTGGT TGCTGACCAT GACAAAAGTG GACCACAGGA GCTGACTGTT
    AAGAGCGGAG ACACTGTGCA ACTGGTCCGA GAGGGAGACG ATGGACGCTG GTTTGTTCGT
    AACCTGCGGA CCAGCAAAGA GGGCTGGGTT CCTGCTGCAA ACCTGCTCAC GCTCATCGGA
    GAGTCCAAAT CTTCTCAGTC TCTCTCCAGC TCAGAAGGCA GTGGGTCTGG AAACCTCAGT
    ACATCGTCCA GCTGCAGTGA AACCTACACA AGCTTCTCGG ACATTAAAGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292252.1
    CDS301..3954
    Translation

    Target ORF information:

    RefSeq Version XM_009293977.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mcf.2 cell line derived transforming sequence-like a (mcf2la), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293977.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTCTGC CTGAGTTTCT ACGGGAAGGC CAGGACGCCC CGCAGAGGAT CTTGTCCCGG 
    CTGACCCAGC TCCTCTACAA CCTGTCGGCT ACAGTCCTGC AGGGAGTCCC CTTCTCTCCT
    TTCACAACCT TCAGCCGCAA GAGCTCCTGG AGAGACTGGA ACGATGAGAT TATGCAGCAG
    GAGAGCAGTC CTCTGTGTGC CGCAGACATC AGCCCAGACC TCAGGAAACA GTTTGCCTTC
    TTGTCTGGTG GCCGAGGACA GAACGGCAGC CCCATCATTA TTTTCCCAGA GTACCCGGAG
    TTTGGTGAGC TGGAGGAGCA GGAGTTTCAC AATGTGCTGA CATACCTGAC CAGCATTCCC
    AGTCTCAGCT CGACGGGGAT CGGCTTCATC CTGGTCATTG ACCGCCGGCA AGACAGATGG
    GCATCAGTGA AGGGGACACT GCTGCGGATC GCGGGCTCTT TCCCAGGCAA CCTGCAGCTG
    GTGCTGGTAC TGAGACCCAC AACACTCTTC CAGCGCACAA TCTCAGACAT ATTATTTAAA
    TTCAACAAAG ATGACTTTAA AATGAAGGTG CCGGTGATCA TGCTCAGCTC TGTGACTGAG
    CTCCACAGTT ACATCGACCG CTCTCAGCTC ACTCAGGAAC TGGGCGGCAC GCAGGAATAC
    TGCCACGAGA AGTGGATTTC ACACCGCACC GCCATTGAGG GTTTTGCTCT GATGGTGAAG
    AAAACAGCAC AGACTTTGCA GTCTTTCGGG ACGGAGCTGG CGGAGACTGA ACTGCCTAAT
    GATGTTGAGG CCACGTCCAA CCTTCTTACC GTCCACACCG AGAAGAAGGA CAAAATGAAG
    GAGGACCTCA GGATCGCTCT CTGTCAGGGA GGACGTTTGC TGGAGAGCAT CAATGAGCCG
    CTGGTGAAAG ATCCAGATTA TAGCATGAAC CACGATGAAC TGGAGAACCT GGCTACTGTT
    CAGAGGTTGC TCGGTCAGCT AGATGAGACT GAAACGGCAT TTGATGATTT CTGGGATAAA
    CATCAGACCA AGCTGGAGCA ATGTCTGCAA CTGCGGCACT TTGAGCAGAA CTTCCGGGAG
    GTTAGAGCTC ACCTAGACCT GGTTTACGAC CGACTATCAG GATTCTCCGA AGTAGGAATC
    AGCCCGGCCC ATGCTGAACA CATCCTCAAA GAACTGACCA ATCACGAAGA GAGAGCATCC
    GAGGTGATGG ACCAGGCTCT GTCTCTTGCG AGTGATGCAG ATCAGCTGAT TGAAGCGTCT
    CATTATGCTG TGGACTCCAT CCTGCCCAAA TGCTCTGAGC TGCGCTCTGT GTGTGAGGAG
    ATCAGCACCA TCCTGAGGGC CAAGAAAGCC TATTTGTTCA AATCCGTGGA GCTTCACCAG
    TGTCTGGAGA AGGCCACTAA ATGGTATGAT GATGGAATCT ACCTGCTGGC GTCTCAGCCT
    GTGGATAAGT GTCAGTCACA GGATGGGGCG GAGTCAGCGC TGCAAGAGAT TGAACGTTAC
    TTGGATACAG CCAATCAGCA CAAGCTAACA GATTTGAATG GAATTTGGAG GGACTATGAT
    TCCGTACTCA CACAAGACTT CAGGGATCAG GTTGACAAAG TGTTTCAGAA GCAGCTGTCC
    ATGCAGGAAA TGTTTGAGAA GAGAAGGGTC AGTCTGAAGA AGCTAGCAGC TAAACAAACT
    CGACCCGTGC AGCCTGTCGC TCCACGTCCT GAAGCCATCA TCAAAACACC AGTCTCATCT
    CCAGCTCATA GAGAGAAGTC TATAGAAGGC GACGTCATCA ATGGAAACTG CAAAAAAGGA
    GAGCTGCATT TACAGAACGA GGGCAGCAGC AGACACCCGT CCGTCTCTGA TGAAGAGGAG
    AACTTCGCTG TGCTCAGACG GCACGTGATG AACGAGCTGC TGGAGACAGA GAGAGCGTAT
    GTGGAGGAGC TGCTGTGCGT GTTGGAGGGT TATGCAGCAG AAATGGACAA TCCTGCCATG
    TCTCACCTCA TCCCCAACAC ACTGCTTCAC AAGAAGGACA TCCTCTTCGG AAACATGTCT
    GAGATCTACC AGTTCCACAA GAAGACTTTT CTTCGTGAAC TGGAGGCTTA CACAGATTAT
    CCTGAGCTTG TGGGACAGTG TTTCTTGGAA AGAATGACAG ATCTGCAGAT TTATGAGAAG
    TACTGTCAGA ACAAACCTCG CTCAGAGTCT TTATGGAGAC AGTGCTCTGA CTGCGCTTTC
    TTCCAGGAGT GTCAAAAGAA ACTGGAACAT AAGCTTGGTC TGGACTCATA CCTGCTGAAA
    CCTGTGCAGA GAATAACCAA ATACCAGCTT TTACTAAAGG AGCTGCTGAA GTACAGTAAA
    GGCTGTGAGG GTGAGGAGGA CCTACAGGAG GCGCTCTCAT CCATTCTTGG CATCCTAAAA
    GCTGTGAACG ACTCTATGCA CCTCATCGCC ATCACTGGAT ACGAAGGTAA CCTCAGTGAT
    CTGGGCCGTC TGATGATGCA GGGCTCCTTC AGCGTGTGGA CGGAGCATAA GAAGGGTCAC
    GCCAAGGTAA AAGACCTGGC ACGATTCAAG CCCATGCAGA GACACCTGTT CCTGCACGAG
    AAAGCTCTGC TCTTCTGTAA GAAACGAGAG GAGAACGGGG AGGGTTATGA GAAAGCCCCG
    TCATACAGCT TCAAACACTC GCTCAGTATG AGTGCTGTGG GTATTACAGA GAACGCCAAA
    GGAGACAACA AAAAGTTTGA GATCTGGTGT AATTCTCGAG AGGAAGTGTA TATTGTGCAG
    GCATCAACAC CAGAGATCAA AACAGCATGG GTGAATGAGA TCCGGAAGGT TCTGACAAGG
    CAGCTAAAGG CCTGCAGGGA GGCGAGCCAG CAGAAGAGCT CCGATCAGAT CTCCCCATTA
    ACTCCCAGCA GCACCGGCCT GACCCTGAGC CCCTTCAAAA CAAACCCAAA GAGCCTGAAG
    AAAGGAGACG AGAAGAAAAC AGAACCCACG ACCACAGATC CCAGCTCAGC CGCCTTATTC
    AAAAACAACG ACAAGCTCAA AGACGAGACT GTCGTCAGTC CCAACACTGA GAGAGCTGCA
    GTGGCTAAAA AGCGCTTTAC ACTGCAGGGC TTCAGTAACC TCAAGAGTCA AAAAGAGCTG
    CTAGCGGCCT CTGGTGGTAA AAGTCTAACA CTGCCCATGG TCACGCTCTG TCATCCAGGA
    TCATCCCCCC CTAGTCCCGA CCACAAAACT AAAAGACAGA GTGATCCTAC ACCGTTTGGC
    TTCAGAGGCT TCACCAAGGC GTCGCTGTCT ATGGATGCGT CTGAAGAAAA CGACGGATAC
    TCGAGCGCCG AGGACCCCAT GAACTCTGAC CCTGAGGATG AGGGAGGGAA GAAATTGTCT
    CCAGGCAAGT ACGTAGTGGT TGCTGACCAT GACAAAAGTG GACCACAGGA GCTGACTGTT
    AAGAGCGGAG ACACTGTGCA ACTGGTCCGA GAGGGAGACG ATGGACGCTG GTTTGTTCGT
    AACCTGCGGA CCAGCAAAGA GGGCTGGGTT CCTGCTGCAA ACCTGCTCAC GCTCATCGGA
    GAGTCCAAAT CTTCTCAGTC TCTCTCCAGC TCAGAAGGCA GTGGGTCTGG AAACCTCAGT
    ACATCGTCCA GCTGCAGTGA AACCTACACA AGCTTCTCGG ACATTAAAGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.