nfasca cDNA ORF clone, Danio rerio(zebrafish)

The following nfasca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nfasca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa147838 XM_021479537.1
Latest version!
Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa54243 XM_017358188.1
Latest version!
Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa54244 XM_017358189.1
Latest version!
Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa35553 NM_001145767.1
Latest version!
Danio rerio neurofascin homolog (chicken) a (nfasca), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa147837 XM_021479536.1
Latest version!
Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa54238 XM_017358183.1
Latest version!
Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa54238 XM_017358182.1
Latest version!
Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa54239 XM_017358184.1
Latest version!
Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa54240 XM_017358185.1
Latest version!
Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa54241 XM_017358186.1
Latest version!
Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa54242 XM_017358187.1
Latest version!
Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa147838
    Clone ID Related Accession (Same CDS sequence) XM_021479537.1
    Accession Version XM_021479537.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAATGCGTAA TATACCGGAA
    ACCCAGCCCA CCTTTCTATC TCCAACGGGC TTGTCCAGCA CCAAGATGGT GCTCAGAGGA
    GAAGAACTTC TCCTGGAGTG TATAGCAGCT GGAGTACCAA CTCCTACCAT TACCTGGTCC
    AAAAGAGGAG GAGACCTGCC AACGAAAAAA GTCAGGATTG GGAACTTCGA TAAAACCTTA
    CGCATTCTCA ATGTGTCTGA GGAAGACATT GGCAGCTACA CCTGCATGGC CAGCAACAGA
    ATTGGCAGCA TTCGCCACAC AGTTGAAGTC CAGGTCAAAG CTGCTCCATT TTGGCTCGAA
    AAGCCCATCA ATCTGGTTCT TGCCCCTGAT GAGAGTGGAC GGTTAGTGTG CCGTGCTAAC
    GGGAGTCCCA AACCCACTAT TCAATGGCTG GTGAATGGAG AACCCATTGA AAGTTCACTC
    CCCAACCCCA ATCGGAGAGT TCTTGATGAC ACGATCATAT TTGAGACGGT TCAGTTTGAA
    AGCAGTGCAG TTTACCAGTG TAACGCCTCC AATGAGCACG GTTATCTTCT GGCAAATGCT
    TTCGTCAGTG TTTTGGATAT GGCACCGCGG ATGCTTGGCC CGAAAAACCA GCTGATCAAA
    GTCATTGAAA ACAACAGGAC CTTCCTCGAG TGTCCTTTCT TTGGCTCACC GCTCCCTTTA
    CTTCGCTGGT TTAAGAACGG ACTTGGCAGT GGTTTGGATG GTGGTCACTA CAGGCTATAC
    CACAATGGCA CTTTGGAGAT CAAGCAGGCC CGGACAGAAG ACCAGGGCAC CTACACCTGC
    GTCGCCAGCA ACATTCTGGG CAAGATGGAG AATCAAGTGC GCCTAGAGGT TAAAGAGCCC
    ACAAGAATAG TCCGCGCACC TGAACATAAG ACGGAACCCA GAGGAACCAC AGTTCGATTC
    GACTGTCGAG TTAAACATGA TCCTTCCCTT CCTGCTACAG TCACATGGCT GAAAGATGAA
    AAGCCTCTGA GCTTTAGCTG GATTGGCCGG TTGAAAAAGG ATGATGAGTC CTTGACTATT
    TACAATGTCA ATCCAGACGA TGGAGGAACC TACACATGCA CAGCCAAAAC TGAGATAGAC
    GAGGACACTG CCTCAGCTCG CCTTACTGTT ACAGGTCGAC CCGACCCACC TCAGGATCTG
    GAACTCTCTG ACCCTTCAGC ACGCAGTGTC AGACTCACCT GGGTGCCCGG AAATGAAAAC
    AACAGTCCTG TTACACATTT CCTAGTCCAG TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG
    TGGCAGAACC TGTCAATTTA TGATTGGAAC CTAAACTCAG TTAACCTTCA GCTATCACCA
    TTCGTCAACT ACCAGTTCAG AGTGATTGCG ATCAATGCTG TGGGTCAGAG TCAACCCAGC
    CACCCCTCTG CACGTTATCA GACCAGCGGA GCCTCTCCTG ATGTTGTACC AAGTGGACTT
    AAAGGGTGGG GAAGCAAAAA AAACAACATG GAGATCACAT GGCAGGCCAT GCAGAACACA
    GAGAGGAATG GGCCAAACCT GCGATATGTG CTCTCCTGGA GAAGAAAAGA CACAGAAGAA
    GAGTGGAATA ACATCACCAC AGCCAAAACA AAACACGTTG TCCATAATAC AGACACCTAC
    GTTCCTTATG AGATCAAAAT ACAGGCTGTC AATGACTTTG GACATGGCCC AGAGTCAAAC
    ATGGTCATCG GTTATTCTGG GGAAGACAAA CCATCCGCTG CACCAACAGA TCTTAGGGTG
    TCAAAGATCA ACAGTACCAA AGTCAATATT CACTGGGAGC CTGTGGAGCA GAGCAGTGTA
    AATGGAGAAT TTAAAGAATA CAAGTTGTAC TACTGGCGGG AGGCCAGCCT CATAAAGGGC
    CTGAAAGTAA ATAAGACAAA GTTGCATAAG GGATTCTTTA CCACAGCAGA ACATCCCTCT
    GGCATCCTGA CAGAGCTCTA TCCATACAGC AAATATAAGA TGTATATGGT GGTTGCTAAC
    CGAGAACATG AAGGGCCGCA AAGCAATACT GTGGAGTTTT ACACCAAGGA GGGAGAGCCA
    GGGGCTCCAA AATACTTTAG GATTCTAAGA CACACGGACG TGGTTCACCT GAAGTGGGAC
    AAACCACTGG AGCCAAACGG TGTCCTGATT GGATACACAC TCCAGTACCA CACAGTGAAC
    GGGACACAGG CGGGAGAGAG GAAAGTCCAG AGTTTCCCAC CCAATGTGAC GGAGTACGTC
    ATGAATCTGC CTCACCGCTT CACGCGCTTC AAGTTCTTCC TAGCAGCCCG TACGCAGGTC
    GGAGCAGGAG AAGTGTACGC TGTCGAGTCT CCACACTTCA CCAATGAAGC CTATAAGGAG
    CAGGAAGACA TCGCTACTCA GGGCTGGTTC ATTGGGTTGA TGTGTGCCGT CGCTTTATTG
    GTTCTGATTC TGCTCATTGT TTGTTTCATC AAGAGGAGTC GTGGAGGAAA ATACCCAGTG
    AGAGAGAAGA AAGACCTCCC CTTAGATCCC GTCGATCATA AAGACCAAGA TGGATCATTT
    GACTATCAGA ACGAGAACAG GTCTCTTGAA AGGGAAAGCA GCGACGAAGA CAACAAGCCG
    TTACAGGGCA GCCAAACGTC CCTGGACGGG AACGTTAAAG AAAGCGACGA CAGTCTCGTG
    GACTACGGCG AAGGAGGAGA CGGACAATTC AACGAGGATG GTTCCTTCAT CGGCCAATAC
    ACAGTAAAGA AGGACAAAGA GGAGACAGAA GGAAATGAGA GCTCAGAAGC CACATCACCT
    GTCAATGCCA TCTACTCTTT GGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335212.1
    CDS317..3823
    Translation

    Target ORF information:

    RefSeq Version XM_021479537.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAATGCGTAA TATACCGGAA
    ACCCAGCCCA CCTTTCTATC TCCAACGGGC TTGTCCAGCA CCAAGATGGT GCTCAGAGGA
    GAAGAACTTC TCCTGGAGTG TATAGCAGCT GGAGTACCAA CTCCTACCAT TACCTGGTCC
    AAAAGAGGAG GAGACCTGCC AACGAAAAAA GTCAGGATTG GGAACTTCGA TAAAACCTTA
    CGCATTCTCA ATGTGTCTGA GGAAGACATT GGCAGCTACA CCTGCATGGC CAGCAACAGA
    ATTGGCAGCA TTCGCCACAC AGTTGAAGTC CAGGTCAAAG CTGCTCCATT TTGGCTCGAA
    AAGCCCATCA ATCTGGTTCT TGCCCCTGAT GAGAGTGGAC GGTTAGTGTG CCGTGCTAAC
    GGGAGTCCCA AACCCACTAT TCAATGGCTG GTGAATGGAG AACCCATTGA AAGTTCACTC
    CCCAACCCCA ATCGGAGAGT TCTTGATGAC ACGATCATAT TTGAGACGGT TCAGTTTGAA
    AGCAGTGCAG TTTACCAGTG TAACGCCTCC AATGAGCACG GTTATCTTCT GGCAAATGCT
    TTCGTCAGTG TTTTGGATAT GGCACCGCGG ATGCTTGGCC CGAAAAACCA GCTGATCAAA
    GTCATTGAAA ACAACAGGAC CTTCCTCGAG TGTCCTTTCT TTGGCTCACC GCTCCCTTTA
    CTTCGCTGGT TTAAGAACGG ACTTGGCAGT GGTTTGGATG GTGGTCACTA CAGGCTATAC
    CACAATGGCA CTTTGGAGAT CAAGCAGGCC CGGACAGAAG ACCAGGGCAC CTACACCTGC
    GTCGCCAGCA ACATTCTGGG CAAGATGGAG AATCAAGTGC GCCTAGAGGT TAAAGAGCCC
    ACAAGAATAG TCCGCGCACC TGAACATAAG ACGGAACCCA GAGGAACCAC AGTTCGATTC
    GACTGTCGAG TTAAACATGA TCCTTCCCTT CCTGCTACAG TCACATGGCT GAAAGATGAA
    AAGCCTCTGA GCTTTAGCTG GATTGGCCGG TTGAAAAAGG ATGATGAGTC CTTGACTATT
    TACAATGTCA ATCCAGACGA TGGAGGAACC TACACATGCA CAGCCAAAAC TGAGATAGAC
    GAGGACACTG CCTCAGCTCG CCTTACTGTT ACAGGTCGAC CCGACCCACC TCAGGATCTG
    GAACTCTCTG ACCCTTCAGC ACGCAGTGTC AGACTCACCT GGGTGCCCGG AAATGAAAAC
    AACAGTCCTG TTACACATTT CCTAGTCCAG TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG
    TGGCAGAACC TGTCAATTTA TGATTGGAAC CTAAACTCAG TTAACCTTCA GCTATCACCA
    TTCGTCAACT ACCAGTTCAG AGTGATTGCG ATCAATGCTG TGGGTCAGAG TCAACCCAGC
    CACCCCTCTG CACGTTATCA GACCAGCGGA GCCTCTCCTG ATGTTGTACC AAGTGGACTT
    AAAGGGTGGG GAAGCAAAAA AAACAACATG GAGATCACAT GGCAGGCCAT GCAGAACACA
    GAGAGGAATG GGCCAAACCT GCGATATGTG CTCTCCTGGA GAAGAAAAGA CACAGAAGAA
    GAGTGGAATA ACATCACCAC AGCCAAAACA AAACACGTTG TCCATAATAC AGACACCTAC
    GTTCCTTATG AGATCAAAAT ACAGGCTGTC AATGACTTTG GACATGGCCC AGAGTCAAAC
    ATGGTCATCG GTTATTCTGG GGAAGACAAA CCATCCGCTG CACCAACAGA TCTTAGGGTG
    TCAAAGATCA ACAGTACCAA AGTCAATATT CACTGGGAGC CTGTGGAGCA GAGCAGTGTA
    AATGGAGAAT TTAAAGAATA CAAGTTGTAC TACTGGCGGG AGGCCAGCCT CATAAAGGGC
    CTGAAAGTAA ATAAGACAAA GTTGCATAAG GGATTCTTTA CCACAGCAGA ACATCCCTCT
    GGCATCCTGA CAGAGCTCTA TCCATACAGC AAATATAAGA TGTATATGGT GGTTGCTAAC
    CGAGAACATG AAGGGCCGCA AAGCAATACT GTGGAGTTTT ACACCAAGGA GGGAGAGCCA
    GGGGCTCCAA AATACTTTAG GATTCTAAGA CACACGGACG TGGTTCACCT GAAGTGGGAC
    AAACCACTGG AGCCAAACGG TGTCCTGATT GGATACACAC TCCAGTACCA CACAGTGAAC
    GGGACACAGG CGGGAGAGAG GAAAGTCCAG AGTTTCCCAC CCAATGTGAC GGAGTACGTC
    ATGAATCTGC CTCACCGCTT CACGCGCTTC AAGTTCTTCC TAGCAGCCCG TACGCAGGTC
    GGAGCAGGAG AAGTGTACGC TGTCGAGTCT CCACACTTCA CCAATGAAGC CTATAAGGAG
    CAGGAAGACA TCGCTACTCA GGGCTGGTTC ATTGGGTTGA TGTGTGCCGT CGCTTTATTG
    GTTCTGATTC TGCTCATTGT TTGTTTCATC AAGAGGAGTC GTGGAGGAAA ATACCCAGTG
    AGAGAGAAGA AAGACCTCCC CTTAGATCCC GTCGATCATA AAGACCAAGA TGGATCATTT
    GACTATCAGA ACGAGAACAG GTCTCTTGAA AGGGAAAGCA GCGACGAAGA CAACAAGCCG
    TTACAGGGCA GCCAAACGTC CCTGGACGGG AACGTTAAAG AAAGCGACGA CAGTCTCGTG
    GACTACGGCG AAGGAGGAGA CGGACAATTC AACGAGGATG GTTCCTTCAT CGGCCAATAC
    ACAGTAAAGA AGGACAAAGA GGAGACAGAA GGAAATGAGA GCTCAGAAGC CACATCACCT
    GTCAATGCCA TCTACTCTTT GGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54243
    Clone ID Related Accession (Same CDS sequence) XM_017358188.1
    Accession Version XM_017358188.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofascin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGAGAATGAC AGCTCGTCTG CTTCAAATCG TAGTGCCTCT
    GCAGGTCGAC CCGACCCACC TCAGGATCTG GAACTCTCTG ACCCTTCAGC ACGCAGTGTC
    AGACTCACCT GGGTGCCCGG AAATGAAAAC AACAGTCCTG TTACACATTT CCTAGTCCAG
    TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG TGGCAGAACC TGTCAATTTA TGATTGGAAC
    CTAAACTCAG TTAACCTTCA GCTATCACCA TTCGTCAACT ACCAGTTCAG AGTGATTGCG
    ATCAATGCTG TGGGTCAGAG TCAACCCAGC CACCCCTCTG CACGTTATCA GACCAGCGGA
    GCCTCTCCTG ATGTTGTACC AAGTGGACTT AAAGGGTGGG GAAGCAAAAA AAACAACATG
    GAGATCACAT GGCAGGCCAT GCAGAACACA GAGAGGAATG GGCCAAACCT GCGATATGTG
    CTCTCCTGGA GAAGAAAAGA CACAGAAGAA GAGTGGAATA ACATCACCAC AGCCAAAACA
    AAACACGTTG TCCATAATAC AGACACCTAC GTTCCTTATG AGATCAAAAT ACAGGCTGTC
    AATGACTTTG GACATGGCCC AGAGTCAAAC ATGGTCATCG GTTATTCTGG GGAAGACAAA
    CCATCCGCTG CACCAACAGA TCTTAGGGTG TCAAAGATCA ACAGTACCAA AGTCAATATT
    CACTGGGAGC CTGTGGAGCA GAGCAGTGTA AATGGAGAAT TTAAAGAATA CAAGTTGTAC
    TACTGGCGGG AGGCCAGCCT CATAAAGGGC CTGAAAGTAA ATAAGACAAA GTTGCATAAG
    GGATTCTTTA CCACAGCAGA ACATCCCTCT GGCATCCTGA CAGAGCTCTA TCCATACAGC
    AAATATAAGA TGTATATGGT GGTTGCTAAC CGAGAACATG AAGGGCCGCA AAGCAATACT
    GTGGAGTTTT ACACCAAGGA GGGAGAGCCA GGGGCTCCAA AATACTTTAG GATTCTAAGA
    CACACGGACG TGGTTCACCT GAAGTGGGAC AAACCACTGG AGCCAAACGG TGTCCTGATT
    GGATACACAC TCCAGTACCA CACAGTGAAC GGGACACAGG CGGGAGAGAG GAAAGTCCAG
    AGTTTCCCAC CCAATGTGAC GGAGTACGTC ATGAATCTGC CTCACCGCTT CACGCGCTTC
    AAGTTCTTCC TAGCAGCCCG TACGCAGGTC GGAGCAGGAG AAGTGTACGC TGTCGAGTCT
    CCACACTTCA CCAATGAAGC CTATAAGGAG CAGGAAGACA TCGCTACTCA GGGCTGGTTC
    ATTGGGTTGA TGTGTGCCGT CGCTTTATTG GTTCTGATTC TGCTCATTGT TTGTTTCATC
    AAGAGGAGTC GTGGAGGAAA ATACCCAGTG AGAGAGAAGA AAGACCTCCC CTTAGATCCC
    GTCGATCATA AAGACCAAGA TGGATCATTT GACTATCACA GCGACGAAGA CAACAAGCCG
    TTACAGGGCA GCCAAACGTC CCTGGACGGG AACGTTAAAG AAAGCGACGA CAGTCTCGTG
    GACTACGGCG AAGGAGGAGA CGGACAATTC AACGAGGATG GTTCCTTCAT CGGCCAATAC
    ACAGTAAAGA AGGACAAAGA GGAGACAGAA GGAAATGAGA GCTCAGAAGC CACATCACCT
    GTCAATGCCA TCTACTCTTT GGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213677.1
    CDS298..3864
    Translation

    Target ORF information:

    RefSeq Version XM_017358188.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGAGAATGAC AGCTCGTCTG CTTCAAATCG TAGTGCCTCT
    GCAGGTCGAC CCGACCCACC TCAGGATCTG GAACTCTCTG ACCCTTCAGC ACGCAGTGTC
    AGACTCACCT GGGTGCCCGG AAATGAAAAC AACAGTCCTG TTACACATTT CCTAGTCCAG
    TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG TGGCAGAACC TGTCAATTTA TGATTGGAAC
    CTAAACTCAG TTAACCTTCA GCTATCACCA TTCGTCAACT ACCAGTTCAG AGTGATTGCG
    ATCAATGCTG TGGGTCAGAG TCAACCCAGC CACCCCTCTG CACGTTATCA GACCAGCGGA
    GCCTCTCCTG ATGTTGTACC AAGTGGACTT AAAGGGTGGG GAAGCAAAAA AAACAACATG
    GAGATCACAT GGCAGGCCAT GCAGAACACA GAGAGGAATG GGCCAAACCT GCGATATGTG
    CTCTCCTGGA GAAGAAAAGA CACAGAAGAA GAGTGGAATA ACATCACCAC AGCCAAAACA
    AAACACGTTG TCCATAATAC AGACACCTAC GTTCCTTATG AGATCAAAAT ACAGGCTGTC
    AATGACTTTG GACATGGCCC AGAGTCAAAC ATGGTCATCG GTTATTCTGG GGAAGACAAA
    CCATCCGCTG CACCAACAGA TCTTAGGGTG TCAAAGATCA ACAGTACCAA AGTCAATATT
    CACTGGGAGC CTGTGGAGCA GAGCAGTGTA AATGGAGAAT TTAAAGAATA CAAGTTGTAC
    TACTGGCGGG AGGCCAGCCT CATAAAGGGC CTGAAAGTAA ATAAGACAAA GTTGCATAAG
    GGATTCTTTA CCACAGCAGA ACATCCCTCT GGCATCCTGA CAGAGCTCTA TCCATACAGC
    AAATATAAGA TGTATATGGT GGTTGCTAAC CGAGAACATG AAGGGCCGCA AAGCAATACT
    GTGGAGTTTT ACACCAAGGA GGGAGAGCCA GGGGCTCCAA AATACTTTAG GATTCTAAGA
    CACACGGACG TGGTTCACCT GAAGTGGGAC AAACCACTGG AGCCAAACGG TGTCCTGATT
    GGATACACAC TCCAGTACCA CACAGTGAAC GGGACACAGG CGGGAGAGAG GAAAGTCCAG
    AGTTTCCCAC CCAATGTGAC GGAGTACGTC ATGAATCTGC CTCACCGCTT CACGCGCTTC
    AAGTTCTTCC TAGCAGCCCG TACGCAGGTC GGAGCAGGAG AAGTGTACGC TGTCGAGTCT
    CCACACTTCA CCAATGAAGC CTATAAGGAG CAGGAAGACA TCGCTACTCA GGGCTGGTTC
    ATTGGGTTGA TGTGTGCCGT CGCTTTATTG GTTCTGATTC TGCTCATTGT TTGTTTCATC
    AAGAGGAGTC GTGGAGGAAA ATACCCAGTG AGAGAGAAGA AAGACCTCCC CTTAGATCCC
    GTCGATCATA AAGACCAAGA TGGATCATTT GACTATCACA GCGACGAAGA CAACAAGCCG
    TTACAGGGCA GCCAAACGTC CCTGGACGGG AACGTTAAAG AAAGCGACGA CAGTCTCGTG
    GACTACGGCG AAGGAGGAGA CGGACAATTC AACGAGGATG GTTCCTTCAT CGGCCAATAC
    ACAGTAAAGA AGGACAAAGA GGAGACAGAA GGAAATGAGA GCTCAGAAGC CACATCACCT
    GTCAATGCCA TCTACTCTTT GGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54244
    Clone ID Related Accession (Same CDS sequence) XM_017358189.1
    Accession Version XM_017358189.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofascin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGGTCGACCC GACCCACCTC AGGATCTGGA ACTCTCTGAC
    CCTTCAGCAC GCAGTGTCAG ACTCACCTGG GTGCCCGGAA ATGAAAACAA CAGTCCTGTT
    ACACATTTCC TAGTCCAGTT TGAAGAGGAC AGGTGGGAGC CAGGGAAGTG GCAGAACCTG
    TCAATTTATG ATTGGAACCT AAACTCAGTT AACCTTCAGC TATCACCATT CGTCAACTAC
    CAGTTCAGAG TGATTGCGAT CAATGCTGTG GGTCAGAGTC AACCCAGCCA CCCCTCTGCA
    CGTTATCAGA CCAGCGGAGC CTCTCCTGAT GTTGTACCAA GTGGACTTAA AGGGTGGGGA
    AGCAAAAAAA ACAACATGGA GATCACATGG CAGGCCATGC AGAACACAGA GAGGAATGGG
    CCAAACCTGC GATATGTGCT CTCCTGGAGA AGAAAAGACA CAGAAGAAGA GTGGAATAAC
    ATCACCACAG CCAAAACAAA ACACGTTGTC CATAATACAG ACACCTACGT TCCTTATGAG
    ATCAAAATAC AGGCTGTCAA TGACTTTGGA CATGGCCCAG AGTCAAACAT GGTCATCGGT
    TATTCTGGGG AAGACAAACC ATCCGCTGCA CCAACAGATC TTAGGGTGTC AAAGATCAAC
    AGTACCAAAG TCAATATTCA CTGGGAGCCT GTGGAGCAGA GCAGTGTAAA TGGAGAATTT
    AAAGAATACA AGTTGTACTA CTGGCGGGAG GCCAGCCTCA TAAAGGGCCT GAAAGTAAAT
    AAGACAAAGT TGCATAAGGG ATTCTTTACC ACAGCAGAAC ATCCCTCTGG CATCCTGACA
    GAGCTCTATC CATACAGCAA ATATAAGATG TATATGGTGG TTGCTAACCG AGAACATGAA
    GGGCCGCAAA GCAATACTGT GGAGTTTTAC ACCAAGGAGG GAGAGCCAGG GGCTCCAAAA
    TACTTTAGGA TTCTAAGACA CACGGACGTG GTTCACCTGA AGTGGGACAA ACCACTGGAG
    CCAAACGGTG TCCTGATTGG ATACACACTC CAGTACCACA CAGTGAACGG GACACAGGCG
    GGAGAGAGGA AAGTCCAGAG TTTCCCACCC AATGTGACGG AGTACGTCAT GAATCTGCCT
    CACCGCTTCA CGCGCTTCAA GTTCTTCCTA GCAGCCCGTA CGCAGGTCGG AGCAGGAGAA
    GTGTACGCTG TCGAGTCTCC ACACTTCACC AATGAAGCCT ATAAGGAGCA GGAAGACATC
    GCTACTCAGG GCTGGTTCAT TGGGTTGATG TGTGCCGTCG CTTTATTGGT TCTGATTCTG
    CTCATTGTTT GTTTCATCAA GAGGAGTCGT GGAGGAAAAT ACCCAGTGAG AGAGAAGAAA
    GACCTCCCCT TAGATCCCGT CGATCATAAA GACCAAGATG GATCATTTGA CTATCACAGC
    GACGAAGACA ACAAGCCGTT ACAGGGCAGC CAAACGTCCC TGGACGGGAA CGTTAAAGAA
    AGCGACGACA GTCTCGTGGA CTACGGCGAA GGAGGAGACG GACAATTCAA CGAGGATGGT
    TCCTTCATCG GCCAATACAC AGTAAAGAAG GACAAAGAGG AGACAGAAGG AAATGAGAGC
    TCAGAAGCCA CATCACCTGT CAATGCCATC TACTCTTTGG CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213678.1
    CDS298..3822
    Translation

    Target ORF information:

    RefSeq Version XM_017358189.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358189.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGGTCGACCC GACCCACCTC AGGATCTGGA ACTCTCTGAC
    CCTTCAGCAC GCAGTGTCAG ACTCACCTGG GTGCCCGGAA ATGAAAACAA CAGTCCTGTT
    ACACATTTCC TAGTCCAGTT TGAAGAGGAC AGGTGGGAGC CAGGGAAGTG GCAGAACCTG
    TCAATTTATG ATTGGAACCT AAACTCAGTT AACCTTCAGC TATCACCATT CGTCAACTAC
    CAGTTCAGAG TGATTGCGAT CAATGCTGTG GGTCAGAGTC AACCCAGCCA CCCCTCTGCA
    CGTTATCAGA CCAGCGGAGC CTCTCCTGAT GTTGTACCAA GTGGACTTAA AGGGTGGGGA
    AGCAAAAAAA ACAACATGGA GATCACATGG CAGGCCATGC AGAACACAGA GAGGAATGGG
    CCAAACCTGC GATATGTGCT CTCCTGGAGA AGAAAAGACA CAGAAGAAGA GTGGAATAAC
    ATCACCACAG CCAAAACAAA ACACGTTGTC CATAATACAG ACACCTACGT TCCTTATGAG
    ATCAAAATAC AGGCTGTCAA TGACTTTGGA CATGGCCCAG AGTCAAACAT GGTCATCGGT
    TATTCTGGGG AAGACAAACC ATCCGCTGCA CCAACAGATC TTAGGGTGTC AAAGATCAAC
    AGTACCAAAG TCAATATTCA CTGGGAGCCT GTGGAGCAGA GCAGTGTAAA TGGAGAATTT
    AAAGAATACA AGTTGTACTA CTGGCGGGAG GCCAGCCTCA TAAAGGGCCT GAAAGTAAAT
    AAGACAAAGT TGCATAAGGG ATTCTTTACC ACAGCAGAAC ATCCCTCTGG CATCCTGACA
    GAGCTCTATC CATACAGCAA ATATAAGATG TATATGGTGG TTGCTAACCG AGAACATGAA
    GGGCCGCAAA GCAATACTGT GGAGTTTTAC ACCAAGGAGG GAGAGCCAGG GGCTCCAAAA
    TACTTTAGGA TTCTAAGACA CACGGACGTG GTTCACCTGA AGTGGGACAA ACCACTGGAG
    CCAAACGGTG TCCTGATTGG ATACACACTC CAGTACCACA CAGTGAACGG GACACAGGCG
    GGAGAGAGGA AAGTCCAGAG TTTCCCACCC AATGTGACGG AGTACGTCAT GAATCTGCCT
    CACCGCTTCA CGCGCTTCAA GTTCTTCCTA GCAGCCCGTA CGCAGGTCGG AGCAGGAGAA
    GTGTACGCTG TCGAGTCTCC ACACTTCACC AATGAAGCCT ATAAGGAGCA GGAAGACATC
    GCTACTCAGG GCTGGTTCAT TGGGTTGATG TGTGCCGTCG CTTTATTGGT TCTGATTCTG
    CTCATTGTTT GTTTCATCAA GAGGAGTCGT GGAGGAAAAT ACCCAGTGAG AGAGAAGAAA
    GACCTCCCCT TAGATCCCGT CGATCATAAA GACCAAGATG GATCATTTGA CTATCACAGC
    GACGAAGACA ACAAGCCGTT ACAGGGCAGC CAAACGTCCC TGGACGGGAA CGTTAAAGAA
    AGCGACGACA GTCTCGTGGA CTACGGCGAA GGAGGAGACG GACAATTCAA CGAGGATGGT
    TCCTTCATCG GCCAATACAC AGTAAAGAAG GACAAAGAGG AGACAGAAGG AAATGAGAGC
    TCAGAAGCCA CATCACCTGT CAATGCCATC TACTCTTTGG CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa35553
    Clone ID Related Accession (Same CDS sequence) NM_001145767.1
    Accession Version NM_001145767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product neurofascin precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ669144.1. On Mar 12, 2009 this sequence version replaced XM_001923293.1. ##Evidence-Data-START## Transcript exon combination :: FJ669144.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA708836, SAMEA898404 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGCG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCCTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTGGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAATGCGTAA TATACCGGAA
    ACCCAGCCCA CCTCTCTATC TCCAACGGGC TTGCCCAGCA CCAAGATGGT GCTCAGAGGA
    GGAGAACTTC TCCTGGAGTG TATAGCAGCT GGAGTACCAA CTCCTACCAT TACCTGGTCC
    AAAAGAGGAG GAGACCTGCC AACGAAAAAA GTCAGGATTG GGAACTTCGA TAAGACCTTA
    CGCATTCTCA ATGTGTCTGA GGAAGACATT GGCAGCTACA CCTGCATGGC CAGCAACAGA
    ATTGGCAGCA TTCGCCACAC AGTTGAAGTC CAGGTCAAAG CTGCTCCATT TTGGCTAGAA
    AAGCCCATCA ATCTGGTTCT TGCCCCTGAT GAGAGTGGAC GGTTAGTGTG CCGTGCTAAC
    GGGAGTCCCA AACCCACTAT TCAGTGGCTG GTGAATGGAG AACCCATTGA AAGTTCACTG
    CCCAACCCCA ATCGGAGAGT TCTTGATGAC ACGATCATAT TTGAGACGGT TCAGTTTGAA
    AGCAGTGCAG TTTACCAGTG TAACGCCTCC AATGAGCACG GTTATCTTCT GGCAAATGCT
    TTCGTCAGTG TTTTGGATAT GGCACCGCGG ATGCTTGGCC CGAAAAACCA GCTGATCAAA
    GTCATTGAAA ACAACAGGAC CTTCCTCGAG TGTCCTTTCT TTGGCTCACC GCTCCCTTTA
    CTTCGCTGGT TTAAGAATGG ACTTGGCAGT GGTTTGGATG GTGGTCACTA CAGGCTATAC
    CACAATGGCA CTTTGGAGAT CAAGCAGGCC CGGACAGAAG ACCAGGGCAC CTACACCTGC
    GTCGCCAGCA GCATTCTGGG CAAGATGGAG AATCAAGTGC GCCTAGAGGT TAAAGAGCCC
    ACAAGAATAG TCCGCGCACC TGAACATAAG ACGGAACCCA GAGGAACCAC AGTTCGATTC
    GACTGTCGAG TTAAACATGA TCCTTCCCTT CCTGCTACAG TCACATGGCT GAAAGATGAA
    AAGCCTCTGA GCTTCAGCTG GATTGGCCGG TTGAAAAAGG ATGATGAGTC CTTGACTATT
    TACAATGTCA ATCCAGACGA TGGAGGAACC TACACATGCA CAGCCAAAAC TGAGATAGAC
    GAGGACACTG CCTCAGCTCG CCTTACTGTT ACAGAGAATG ACAGCTCGTC TGCTTCAAAT
    CGTAGTGCCT CTGCAGGTCG ACCCGACCCA CCTCAGGATC TGGAACTCTC GGACCCTTCA
    GCACGCAGTG TCAGACTCAC CTGGGTGCCC GGAAATGAAA ACAATAGTCC TGTTACACAT
    TTCCTAGTCC AGTTTGAAGA GGACAGGTGG GAGCCAGGGA AGTGGCAGAA CCTGTCAATT
    TATGATTGGA ACCTAAACTC AGTTAACCTT CAGCTATCAC CATTCGTCAA CTACCAGTTC
    AGAGTAATTG CGATCAATGC TGTGGGTCAG AGTCAACCCA GCCACCCCTC TGCACGTTAT
    CAGACCAGCG GAGCCCCTCC TGATGTTGTA CCAAGTGGAC TTAAAGGGTG GGGAAGCAAA
    AAAAACAACA TGGAGATCAC ATGGCAGGCC ATGCAGAACA CAGAGAGGAA TGGGCCAAAC
    CTGCGATATG TGCTCTCCTG GAGAAGAAAA GACACAGAAG AAGAGTGGAA TAACATCACC
    ACAGCCAAAA CAAAACACGT TGTCCATAAT ACAGACACCT ACGTTCCTTA TGAGATCAAA
    ATACAGGCTG TTAATGACTT TGGACATGGC CCAGAGTCAA ACATGGTCAT CGGTTATTCT
    GGGGAAGACA AACCATCCGC TGCACCAACA GATCTTAGGG TGTCAAAGAT CAACAGTACC
    AAAGTCAATA TTCACTGGGA GCCTGTGGAG CAGAGCAGTG TGAATGGAGA ATTTAAAGAA
    TACAAGTTGT ACTACTGGCG GGAGGCCAGC CTCATAAAGG GCCTGAAAGT AAATAGGACA
    AAGTTGCATA AGGGATTCTT TACCACAGCA GAAAATCCCT CTGGCATCCT AACAGAGCTC
    TATCCATACA GCAAATATAA GATGTATATG GTGGTTGCTA ACCGAGAACA TGAAGGGCCG
    CAGAGCAATA CTGTGGAGTT TTACACCAAG GAGGGAGAGC CAGGGGCTCC AAAATACTTT
    AGGATTCTAA GGCACACGGA CGTGGTTCAC CTGAAGTGGG ACAAACCACT GGAGCCAAAC
    GGTGTCCTGA TTGGATACAC ACTCCAGTAC CACACAGTGA ACGGGACACA GACGGGAGAG
    AGGAAAGTCC AGAGTTTCCC ACCCAATGTG ACGGAGTACG TCATGAATCT GCCTCACCGC
    TTCACGCGCT TCAAGTTCTT CCTAGCAGCC CGTACGCAGG TCGGAGCAGG AGAAGTGTAC
    GCTGTCGAGT CTCCACACTT CACAAATGAA GGCACCCCAG ATACTGACTC TACAGTTGTA
    GCCGTTAACT TTACAGATGG CTTGATGTTC GAAATTCCTC CTCCTCCCCT TCCCCCCTCC
    ACTGACCCAT ACCCAACAAT ATTCACCCTA GAACCAACTC CACCAGTCCC TACAACTGCT
    GCTCCAACCA AACCTACTAC CACCACCACC ACCACCACCA CTACTACTAC AACTTCTACT
    ACTACTACAA CTACCACAAC TACTACAACA ACCACCACTA CTACAGCAGC TTCCACCACA
    GTTGCTCCAG TTCGGCCCGT GCCTCCCAAA ACAGTGGATT GGAAAATGCT GGCCCCGAAT
    GTAAAGATCT GGAATCTGAC GGTGGATGCT AACAATGACA ATGCTAACGT CAGCTGGAAG
    CACAACTTCT CCGTTGACAG CAGCAAGTTT GTTGTAGAGT TCACACTGGA CAGTAATGGG
    TCGATGACGA GCATTCAAGT AAACCAGCAG CCTCACATTA AGCTGGCTGG GCTGGTGGCT
    GGGGCCAAGT ATCGGTTGCG GGTCTATTCG CATGAGCACA ACTCCATCAG CAGCGACTAT
    GTCACGTTCG AGACAGGCAG AGCCTATAAG GAGCAGGAAG ACATCGCTAC TCAGGGCTGG
    TTCATTGGGT TGATGTGTGC CGTCGCTTTA TTGGTTCTGA TTCTGCTCAT TGTTTGTTTC
    ATCAAGAGGA GTCGTGGAGG AAAATACCCA GTAAGAGAGA AGAAAGACCT CCCCTTAGAT
    CCCGTCGATC ATAAAGACCA AGATGGATCA TTTGACTATC ACAGCGACGA AGACAACAAG
    CCGTTACAGG GCAGCCAAAC GTCCCTGGAC GGGAACGTTA AAGAAAGCGA CGACAGTCTC
    GTGGACTACG GCGAAGGAGG AGATGGACAA TTCAACGAGG ATGGTTCCTT CATCGGCCAA
    TACACAGTAA AGAAGGACAA AGAGGAGACA GAAGGAAATG AGAGCTCAGA AGCCACATCA
    CCTGTCAATG CCATCTACTC TTTGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139239.1
    CDS1..4110
    Translation

    Target ORF information:

    RefSeq Version NM_001145767.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofascin homolog (chicken) a (nfasca), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGCG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCCTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTGGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAATGCGTAA TATACCGGAA
    ACCCAGCCCA CCTCTCTATC TCCAACGGGC TTGCCCAGCA CCAAGATGGT GCTCAGAGGA
    GGAGAACTTC TCCTGGAGTG TATAGCAGCT GGAGTACCAA CTCCTACCAT TACCTGGTCC
    AAAAGAGGAG GAGACCTGCC AACGAAAAAA GTCAGGATTG GGAACTTCGA TAAGACCTTA
    CGCATTCTCA ATGTGTCTGA GGAAGACATT GGCAGCTACA CCTGCATGGC CAGCAACAGA
    ATTGGCAGCA TTCGCCACAC AGTTGAAGTC CAGGTCAAAG CTGCTCCATT TTGGCTAGAA
    AAGCCCATCA ATCTGGTTCT TGCCCCTGAT GAGAGTGGAC GGTTAGTGTG CCGTGCTAAC
    GGGAGTCCCA AACCCACTAT TCAGTGGCTG GTGAATGGAG AACCCATTGA AAGTTCACTG
    CCCAACCCCA ATCGGAGAGT TCTTGATGAC ACGATCATAT TTGAGACGGT TCAGTTTGAA
    AGCAGTGCAG TTTACCAGTG TAACGCCTCC AATGAGCACG GTTATCTTCT GGCAAATGCT
    TTCGTCAGTG TTTTGGATAT GGCACCGCGG ATGCTTGGCC CGAAAAACCA GCTGATCAAA
    GTCATTGAAA ACAACAGGAC CTTCCTCGAG TGTCCTTTCT TTGGCTCACC GCTCCCTTTA
    CTTCGCTGGT TTAAGAATGG ACTTGGCAGT GGTTTGGATG GTGGTCACTA CAGGCTATAC
    CACAATGGCA CTTTGGAGAT CAAGCAGGCC CGGACAGAAG ACCAGGGCAC CTACACCTGC
    GTCGCCAGCA GCATTCTGGG CAAGATGGAG AATCAAGTGC GCCTAGAGGT TAAAGAGCCC
    ACAAGAATAG TCCGCGCACC TGAACATAAG ACGGAACCCA GAGGAACCAC AGTTCGATTC
    GACTGTCGAG TTAAACATGA TCCTTCCCTT CCTGCTACAG TCACATGGCT GAAAGATGAA
    AAGCCTCTGA GCTTCAGCTG GATTGGCCGG TTGAAAAAGG ATGATGAGTC CTTGACTATT
    TACAATGTCA ATCCAGACGA TGGAGGAACC TACACATGCA CAGCCAAAAC TGAGATAGAC
    GAGGACACTG CCTCAGCTCG CCTTACTGTT ACAGAGAATG ACAGCTCGTC TGCTTCAAAT
    CGTAGTGCCT CTGCAGGTCG ACCCGACCCA CCTCAGGATC TGGAACTCTC GGACCCTTCA
    GCACGCAGTG TCAGACTCAC CTGGGTGCCC GGAAATGAAA ACAATAGTCC TGTTACACAT
    TTCCTAGTCC AGTTTGAAGA GGACAGGTGG GAGCCAGGGA AGTGGCAGAA CCTGTCAATT
    TATGATTGGA ACCTAAACTC AGTTAACCTT CAGCTATCAC CATTCGTCAA CTACCAGTTC
    AGAGTAATTG CGATCAATGC TGTGGGTCAG AGTCAACCCA GCCACCCCTC TGCACGTTAT
    CAGACCAGCG GAGCCCCTCC TGATGTTGTA CCAAGTGGAC TTAAAGGGTG GGGAAGCAAA
    AAAAACAACA TGGAGATCAC ATGGCAGGCC ATGCAGAACA CAGAGAGGAA TGGGCCAAAC
    CTGCGATATG TGCTCTCCTG GAGAAGAAAA GACACAGAAG AAGAGTGGAA TAACATCACC
    ACAGCCAAAA CAAAACACGT TGTCCATAAT ACAGACACCT ACGTTCCTTA TGAGATCAAA
    ATACAGGCTG TTAATGACTT TGGACATGGC CCAGAGTCAA ACATGGTCAT CGGTTATTCT
    GGGGAAGACA AACCATCCGC TGCACCAACA GATCTTAGGG TGTCAAAGAT CAACAGTACC
    AAAGTCAATA TTCACTGGGA GCCTGTGGAG CAGAGCAGTG TGAATGGAGA ATTTAAAGAA
    TACAAGTTGT ACTACTGGCG GGAGGCCAGC CTCATAAAGG GCCTGAAAGT AAATAGGACA
    AAGTTGCATA AGGGATTCTT TACCACAGCA GAAAATCCCT CTGGCATCCT AACAGAGCTC
    TATCCATACA GCAAATATAA GATGTATATG GTGGTTGCTA ACCGAGAACA TGAAGGGCCG
    CAGAGCAATA CTGTGGAGTT TTACACCAAG GAGGGAGAGC CAGGGGCTCC AAAATACTTT
    AGGATTCTAA GGCACACGGA CGTGGTTCAC CTGAAGTGGG ACAAACCACT GGAGCCAAAC
    GGTGTCCTGA TTGGATACAC ACTCCAGTAC CACACAGTGA ACGGGACACA GACGGGAGAG
    AGGAAAGTCC AGAGTTTCCC ACCCAATGTG ACGGAGTACG TCATGAATCT GCCTCACCGC
    TTCACGCGCT TCAAGTTCTT CCTAGCAGCC CGTACGCAGG TCGGAGCAGG AGAAGTGTAC
    GCTGTCGAGT CTCCACACTT CACAAATGAA GGCACCCCAG ATACTGACTC TACAGTTGTA
    GCCGTTAACT TTACAGATGG CTTGATGTTC GAAATTCCTC CTCCTCCCCT TCCCCCCTCC
    ACTGACCCAT ACCCAACAAT ATTCACCCTA GAACCAACTC CACCAGTCCC TACAACTGCT
    GCTCCAACCA AACCTACTAC CACCACCACC ACCACCACCA CTACTACTAC AACTTCTACT
    ACTACTACAA CTACCACAAC TACTACAACA ACCACCACTA CTACAGCAGC TTCCACCACA
    GTTGCTCCAG TTCGGCCCGT GCCTCCCAAA ACAGTGGATT GGAAAATGCT GGCCCCGAAT
    GTAAAGATCT GGAATCTGAC GGTGGATGCT AACAATGACA ATGCTAACGT CAGCTGGAAG
    CACAACTTCT CCGTTGACAG CAGCAAGTTT GTTGTAGAGT TCACACTGGA CAGTAATGGG
    TCGATGACGA GCATTCAAGT AAACCAGCAG CCTCACATTA AGCTGGCTGG GCTGGTGGCT
    GGGGCCAAGT ATCGGTTGCG GGTCTATTCG CATGAGCACA ACTCCATCAG CAGCGACTAT
    GTCACGTTCG AGACAGGCAG AGCCTATAAG GAGCAGGAAG ACATCGCTAC TCAGGGCTGG
    TTCATTGGGT TGATGTGTGC CGTCGCTTTA TTGGTTCTGA TTCTGCTCAT TGTTTGTTTC
    ATCAAGAGGA GTCGTGGAGG AAAATACCCA GTAAGAGAGA AGAAAGACCT CCCCTTAGAT
    CCCGTCGATC ATAAAGACCA AGATGGATCA TTTGACTATC ACAGCGACGA AGACAACAAG
    CCGTTACAGG GCAGCCAAAC GTCCCTGGAC GGGAACGTTA AAGAAAGCGA CGACAGTCTC
    GTGGACTACG GCGAAGGAGG AGATGGACAA TTCAACGAGG ATGGTTCCTT CATCGGCCAA
    TACACAGTAA AGAAGGACAA AGAGGAGACA GAAGGAAATG AGAGCTCAGA AGCCACATCA
    CCTGTCAATG CCATCTACTC TTTGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147837
    Clone ID Related Accession (Same CDS sequence) XM_021479536.1
    Accession Version XM_021479536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAATGCGTAA TATACCGGAA
    ACCCAGCCCA CCTTTCTATC TCCAACGGGC TTGTCCAGCA CCAAGATGGT GCTCAGAGGA
    GAAGAACTTC TCCTGGAGTG TATAGCAGCT GGAGTACCAA CTCCTACCAT TACCTGGTCC
    AAAAGAGGAG GAGACCTGCC AACGAAAAAA GTCAGGATTG GGAACTTCGA TAAAACCTTA
    CGCATTCTCA ATGTGTCTGA GGAAGACATT GGCAGCTACA CCTGCATGGC CAGCAACAGA
    ATTGGCAGCA TTCGCCACAC AGTTGAAGTC CAGGTCAAAG CTGCTCCATT TTGGCTCGAA
    AAGCCCATCA ATCTGGTTCT TGCCCCTGAT GAGAGTGGAC GGTTAGTGTG CCGTGCTAAC
    GGGAGTCCCA AACCCACTAT TCAATGGCTG GTGAATGGAG AACCCATTGA AAGTTCACTC
    CCCAACCCCA ATCGGAGAGT TCTTGATGAC ACGATCATAT TTGAGACGGT TCAGTTTGAA
    AGCAGTGCAG TTTACCAGTG TAACGCCTCC AATGAGCACG GTTATCTTCT GGCAAATGCT
    TTCGTCAGTG TTTTGGATAT GGCACCGCGG ATGCTTGGCC CGAAAAACCA GCTGATCAAA
    GTCATTGAAA ACAACAGGAC CTTCCTCGAG TGTCCTTTCT TTGGCTCACC GCTCCCTTTA
    CTTCGCTGGT TTAAGAACGG ACTTGGCAGT GGTTTGGATG GTGGTCACTA CAGGCTATAC
    CACAATGGCA CTTTGGAGAT CAAGCAGGCC CGGACAGAAG ACCAGGGCAC CTACACCTGC
    GTCGCCAGCA ACATTCTGGG CAAGATGGAG AATCAAGTGC GCCTAGAGGT TAAAGAGCCC
    ACAAGAATAG TCCGCGCACC TGAACATAAG ACGGAACCCA GAGGAACCAC AGTTCGATTC
    GACTGTCGAG TTAAACATGA TCCTTCCCTT CCTGCTACAG TCACATGGCT GAAAGATGAA
    AAGCCTCTGA GCTTTAGCTG GATTGGCCGG TTGAAAAAGG ATGATGAGTC CTTGACTATT
    TACAATGTCA ATCCAGACGA TGGAGGAACC TACACATGCA CAGCCAAAAC TGAGATAGAC
    GAGGACACTG CCTCAGCTCG CCTTACTGTT ACAGAGAATG ACAGCTCGTC TGCTTCAAAT
    CGTAGTGCCT CTGCAGGTCG ACCCGACCCA CCTCAGGATC TGGAACTCTC TGACCCTTCA
    GCACGCAGTG TCAGACTCAC CTGGGTGCCC GGAAATGAAA ACAACAGTCC TGTTACACAT
    TTCCTAGTCC AGTTTGAAGA GGACAGGTGG GAGCCAGGGA AGTGGCAGAA CCTGTCAATT
    TATGATTGGA ACCTAAACTC AGTTAACCTT CAGCTATCAC CATTCGTCAA CTACCAGTTC
    AGAGTGATTG CGATCAATGC TGTGGGTCAG AGTCAACCCA GCCACCCCTC TGCACGTTAT
    CAGACCAGCG GAGCCTCTCC TGATGTTGTA CCAAGTGGAC TTAAAGGGTG GGGAAGCAAA
    AAAAACAACA TGGAGATCAC ATGGCAGGCC ATGCAGAACA CAGAGAGGAA TGGGCCAAAC
    CTGCGATATG TGCTCTCCTG GAGAAGAAAA GACACAGAAG AAGAGTGGAA TAACATCACC
    ACAGCCAAAA CAAAACACGT TGTCCATAAT ACAGACACCT ACGTTCCTTA TGAGATCAAA
    ATACAGGCTG TCAATGACTT TGGACATGGC CCAGAGTCAA ACATGGTCAT CGGTTATTCT
    GGGGAAGACA AACCATCCGC TGCACCAACA GATCTTAGGG TGTCAAAGAT CAACAGTACC
    AAAGTCAATA TTCACTGGGA GCCTGTGGAG CAGAGCAGTG TAAATGGAGA ATTTAAAGAA
    TACAAGTTGT ACTACTGGCG GGAGGCCAGC CTCATAAAGG GCCTGAAAGT AAATAAGACA
    AAGTTGCATA AGGGATTCTT TACCACAGCA GAACATCCCT CTGGCATCCT GACAGAGCTC
    TATCCATACA GCAAATATAA GATGTATATG GTGGTTGCTA ACCGAGAACA TGAAGGGCCG
    CAAAGCAATA CTGTGGAGTT TTACACCAAG GAGGGAGAGC CAGGGGCTCC AAAATACTTT
    AGGATTCTAA GACACACGGA CGTGGTTCAC CTGAAGTGGG ACAAACCACT GGAGCCAAAC
    GGTGTCCTGA TTGGATACAC ACTCCAGTAC CACACAGTGA ACGGGACACA GGCGGGAGAG
    AGGAAAGTCC AGAGTTTCCC ACCCAATGTG ACGGAGTACG TCATGAATCT GCCTCACCGC
    TTCACGCGCT TCAAGTTCTT CCTAGCAGCC CGTACGCAGG TCGGAGCAGG AGAAGTGTAC
    GCTGTCGAGT CTCCACACTT CACCAATGAA GGCACCCCAG ATACTGACTC TACAGTTGTA
    GCCGTTAACT TTACAGATGG CTTGATGTTC GAAATTCCTC CTCCTCCCCT TCCCCCCTCC
    ACTGACCCAA ACCCAACAAT ATTCACCCCA GAACCAACTC CACCAGTCCC TACAACTGCT
    GCTCCAACCA AACCTACTAC CACCACCACC ACCACCACTA CTACTACTAC AACTTCTACT
    ACTACTACAA CTACCACAAC TACTACAACA ACCACCACTA CTACAGCAGC TTCCACCACA
    GTTGCTCCAG TTCGGCCCGT GCCTCCCAAA ACAGTGGATT GGAAAATGCT GGCCCCGAAT
    GTAAAGATCT GGAATCTGAC GGTGGATGCT AACAATGACA ATGCTAACGT CAGCTGGAAG
    CACAACTTCT CCGTTGACAG CAGCAAGTTT GTTGTAGAGT TCACACTGGA CAGTAATGGG
    TCGATGACGA GCATTCAAGT AAACCAGCAG CCTCACATTA AGCTGGCTGG GCTGGTGGCT
    GGGGCCAAGT ATCGGTTGCG GGTCTATTCG CATGAGCACA ACTCCATCAG CAGCGACTAT
    GTCACGTTCG AGACAGGCAG AGCCTATAAG GAGCAGGAAG ACATCGCTAC TCAGGGCTGG
    TTCATTGGGT TGATGTGTGC CGTCGCTTTA TTGGTTCTGA TTCTGCTCAT TGTTTGTTTC
    ATCAAGAGGA GTCGTGGAGG AAAATACCCA GTGAGAGAGA AGAAAGACCT CCCCTTAGAT
    CCCGTCGATC ATAAAGACCA AGATGGATCA TTTGACTATC AGAACGAGAA CAGGTCTCTT
    GAAAGGGAAA GCAGCGACGA AGACAACAAG CCGTTACAGG GCAGCCAAAC GTCCCTGGAC
    GGGAACGTTA AAGAAAGCGA CGACAGTCTC GTGGACTACG GCGAAGGAGG AGACGGACAA
    TTCAACGAGG ATGGTTCCTT CATCGGCCAA TACACAGTAA AGAAGGACAA AGAGGAGACA
    GAAGGAAATG AGAGCTCAGA AGCCACATCA CCTGTCAATG CCATCTACTC TTTGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335211.1
    CDS413..4552
    Translation

    Target ORF information:

    RefSeq Version XM_021479536.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAATGCGTAA TATACCGGAA
    ACCCAGCCCA CCTTTCTATC TCCAACGGGC TTGTCCAGCA CCAAGATGGT GCTCAGAGGA
    GAAGAACTTC TCCTGGAGTG TATAGCAGCT GGAGTACCAA CTCCTACCAT TACCTGGTCC
    AAAAGAGGAG GAGACCTGCC AACGAAAAAA GTCAGGATTG GGAACTTCGA TAAAACCTTA
    CGCATTCTCA ATGTGTCTGA GGAAGACATT GGCAGCTACA CCTGCATGGC CAGCAACAGA
    ATTGGCAGCA TTCGCCACAC AGTTGAAGTC CAGGTCAAAG CTGCTCCATT TTGGCTCGAA
    AAGCCCATCA ATCTGGTTCT TGCCCCTGAT GAGAGTGGAC GGTTAGTGTG CCGTGCTAAC
    GGGAGTCCCA AACCCACTAT TCAATGGCTG GTGAATGGAG AACCCATTGA AAGTTCACTC
    CCCAACCCCA ATCGGAGAGT TCTTGATGAC ACGATCATAT TTGAGACGGT TCAGTTTGAA
    AGCAGTGCAG TTTACCAGTG TAACGCCTCC AATGAGCACG GTTATCTTCT GGCAAATGCT
    TTCGTCAGTG TTTTGGATAT GGCACCGCGG ATGCTTGGCC CGAAAAACCA GCTGATCAAA
    GTCATTGAAA ACAACAGGAC CTTCCTCGAG TGTCCTTTCT TTGGCTCACC GCTCCCTTTA
    CTTCGCTGGT TTAAGAACGG ACTTGGCAGT GGTTTGGATG GTGGTCACTA CAGGCTATAC
    CACAATGGCA CTTTGGAGAT CAAGCAGGCC CGGACAGAAG ACCAGGGCAC CTACACCTGC
    GTCGCCAGCA ACATTCTGGG CAAGATGGAG AATCAAGTGC GCCTAGAGGT TAAAGAGCCC
    ACAAGAATAG TCCGCGCACC TGAACATAAG ACGGAACCCA GAGGAACCAC AGTTCGATTC
    GACTGTCGAG TTAAACATGA TCCTTCCCTT CCTGCTACAG TCACATGGCT GAAAGATGAA
    AAGCCTCTGA GCTTTAGCTG GATTGGCCGG TTGAAAAAGG ATGATGAGTC CTTGACTATT
    TACAATGTCA ATCCAGACGA TGGAGGAACC TACACATGCA CAGCCAAAAC TGAGATAGAC
    GAGGACACTG CCTCAGCTCG CCTTACTGTT ACAGAGAATG ACAGCTCGTC TGCTTCAAAT
    CGTAGTGCCT CTGCAGGTCG ACCCGACCCA CCTCAGGATC TGGAACTCTC TGACCCTTCA
    GCACGCAGTG TCAGACTCAC CTGGGTGCCC GGAAATGAAA ACAACAGTCC TGTTACACAT
    TTCCTAGTCC AGTTTGAAGA GGACAGGTGG GAGCCAGGGA AGTGGCAGAA CCTGTCAATT
    TATGATTGGA ACCTAAACTC AGTTAACCTT CAGCTATCAC CATTCGTCAA CTACCAGTTC
    AGAGTGATTG CGATCAATGC TGTGGGTCAG AGTCAACCCA GCCACCCCTC TGCACGTTAT
    CAGACCAGCG GAGCCTCTCC TGATGTTGTA CCAAGTGGAC TTAAAGGGTG GGGAAGCAAA
    AAAAACAACA TGGAGATCAC ATGGCAGGCC ATGCAGAACA CAGAGAGGAA TGGGCCAAAC
    CTGCGATATG TGCTCTCCTG GAGAAGAAAA GACACAGAAG AAGAGTGGAA TAACATCACC
    ACAGCCAAAA CAAAACACGT TGTCCATAAT ACAGACACCT ACGTTCCTTA TGAGATCAAA
    ATACAGGCTG TCAATGACTT TGGACATGGC CCAGAGTCAA ACATGGTCAT CGGTTATTCT
    GGGGAAGACA AACCATCCGC TGCACCAACA GATCTTAGGG TGTCAAAGAT CAACAGTACC
    AAAGTCAATA TTCACTGGGA GCCTGTGGAG CAGAGCAGTG TAAATGGAGA ATTTAAAGAA
    TACAAGTTGT ACTACTGGCG GGAGGCCAGC CTCATAAAGG GCCTGAAAGT AAATAAGACA
    AAGTTGCATA AGGGATTCTT TACCACAGCA GAACATCCCT CTGGCATCCT GACAGAGCTC
    TATCCATACA GCAAATATAA GATGTATATG GTGGTTGCTA ACCGAGAACA TGAAGGGCCG
    CAAAGCAATA CTGTGGAGTT TTACACCAAG GAGGGAGAGC CAGGGGCTCC AAAATACTTT
    AGGATTCTAA GACACACGGA CGTGGTTCAC CTGAAGTGGG ACAAACCACT GGAGCCAAAC
    GGTGTCCTGA TTGGATACAC ACTCCAGTAC CACACAGTGA ACGGGACACA GGCGGGAGAG
    AGGAAAGTCC AGAGTTTCCC ACCCAATGTG ACGGAGTACG TCATGAATCT GCCTCACCGC
    TTCACGCGCT TCAAGTTCTT CCTAGCAGCC CGTACGCAGG TCGGAGCAGG AGAAGTGTAC
    GCTGTCGAGT CTCCACACTT CACCAATGAA GGCACCCCAG ATACTGACTC TACAGTTGTA
    GCCGTTAACT TTACAGATGG CTTGATGTTC GAAATTCCTC CTCCTCCCCT TCCCCCCTCC
    ACTGACCCAA ACCCAACAAT ATTCACCCCA GAACCAACTC CACCAGTCCC TACAACTGCT
    GCTCCAACCA AACCTACTAC CACCACCACC ACCACCACTA CTACTACTAC AACTTCTACT
    ACTACTACAA CTACCACAAC TACTACAACA ACCACCACTA CTACAGCAGC TTCCACCACA
    GTTGCTCCAG TTCGGCCCGT GCCTCCCAAA ACAGTGGATT GGAAAATGCT GGCCCCGAAT
    GTAAAGATCT GGAATCTGAC GGTGGATGCT AACAATGACA ATGCTAACGT CAGCTGGAAG
    CACAACTTCT CCGTTGACAG CAGCAAGTTT GTTGTAGAGT TCACACTGGA CAGTAATGGG
    TCGATGACGA GCATTCAAGT AAACCAGCAG CCTCACATTA AGCTGGCTGG GCTGGTGGCT
    GGGGCCAAGT ATCGGTTGCG GGTCTATTCG CATGAGCACA ACTCCATCAG CAGCGACTAT
    GTCACGTTCG AGACAGGCAG AGCCTATAAG GAGCAGGAAG ACATCGCTAC TCAGGGCTGG
    TTCATTGGGT TGATGTGTGC CGTCGCTTTA TTGGTTCTGA TTCTGCTCAT TGTTTGTTTC
    ATCAAGAGGA GTCGTGGAGG AAAATACCCA GTGAGAGAGA AGAAAGACCT CCCCTTAGAT
    CCCGTCGATC ATAAAGACCA AGATGGATCA TTTGACTATC AGAACGAGAA CAGGTCTCTT
    GAAAGGGAAA GCAGCGACGA AGACAACAAG CCGTTACAGG GCAGCCAAAC GTCCCTGGAC
    GGGAACGTTA AAGAAAGCGA CGACAGTCTC GTGGACTACG GCGAAGGAGG AGACGGACAA
    TTCAACGAGG ATGGTTCCTT CATCGGCCAA TACACAGTAA AGAAGGACAA AGAGGAGACA
    GAAGGAAATG AGAGCTCAGA AGCCACATCA CCTGTCAATG CCATCTACTC TTTGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54238
    Clone ID Related Accession (Same CDS sequence) XM_017358182.1 , XM_017358183.1
    Accession Version XM_017358182.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofascin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGAGAATGAC AGCTCGTCTG CTTCAAATCG TAGTGCCTCT
    GCAGGTCGAC CCGACCCACC TCAGGATCTG GAACTCTCTG ACCCTTCAGC ACGCAGTGTC
    AGACTCACCT GGGTGCCCGG AAATGAAAAC AACAGTCCTG TTACACATTT CCTAGTCCAG
    TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG TGGCAGAACC TGTCAATTTA TGATTGGAAC
    CTAAACTCAG TTAACCTTCA GCTATCACCA TTCGTCAACT ACCAGTTCAG AGTGATTGCG
    ATCAATGCTG TGGGTCAGAG TCAACCCAGC CACCCCTCTG CACGTTATCA GACCAGCGGA
    GCCTCTCCTG ATGTTGTACC AAGTGGACTT AAAGGGTGGG GAAGCAAAAA AAACAACATG
    GAGATCACAT GGCAGGCCAT GCAGAACACA GAGAGGAATG GGCCAAACCT GCGATATGTG
    CTCTCCTGGA GAAGAAAAGA CACAGAAGAA GAGTGGAATA ACATCACCAC AGCCAAAACA
    AAACACGTTG TCCATAATAC AGACACCTAC GTTCCTTATG AGATCAAAAT ACAGGCTGTC
    AATGACTTTG GACATGGCCC AGAGTCAAAC ATGGTCATCG GTTATTCTGG GGAAGACAAA
    CCATCCGCTG CACCAACAGA TCTTAGGGTG TCAAAGATCA ACAGTACCAA AGTCAATATT
    CACTGGGAGC CTGTGGAGCA GAGCAGTGTA AATGGAGAAT TTAAAGAATA CAAGTTGTAC
    TACTGGCGGG AGGCCAGCCT CATAAAGGGC CTGAAAGTAA ATAAGACAAA GTTGCATAAG
    GGATTCTTTA CCACAGCAGA ACATCCCTCT GGCATCCTGA CAGAGCTCTA TCCATACAGC
    AAATATAAGA TGTATATGGT GGTTGCTAAC CGAGAACATG AAGGGCCGCA AAGCAATACT
    GTGGAGTTTT ACACCAAGGA GGGAGAGCCA GGGGCTCCAA AATACTTTAG GATTCTAAGA
    CACACGGACG TGGTTCACCT GAAGTGGGAC AAACCACTGG AGCCAAACGG TGTCCTGATT
    GGATACACAC TCCAGTACCA CACAGTGAAC GGGACACAGG CGGGAGAGAG GAAAGTCCAG
    AGTTTCCCAC CCAATGTGAC GGAGTACGTC ATGAATCTGC CTCACCGCTT CACGCGCTTC
    AAGTTCTTCC TAGCAGCCCG TACGCAGGTC GGAGCAGGAG AAGTGTACGC TGTCGAGTCT
    CCACACTTCA CCAATGAAGG CACCCCAGAT ACTGACTCTA CAGTTGTAGC CGTTAACTTT
    ACAGATGGCT TGATGTTCGA AATTCCTCCT CCTCCCCTTC CCCCCTCCAC TGACCCAAAC
    CCAACAATAT TCACCCCAGA ACCAACTCCA CCAGTCCCTA CAACTGCTGC TCCAACCAAA
    CCTACTACCA CCACCACCAC CACCACTACT ACTACTACAA CTTCTACTAC TACTACAACT
    ACCACAACTA CTACAACAAC CACCACTACT ACAGCAGCTT CCACCACAGT TGCTCCAGTT
    CGGCCCGTGC CTCCCAAAAC AGTGGATTGG AAAATGCTGG CCCCGAATGT AAAGATCTGG
    AATCTGACGG TGGATGCTAA CAATGACAAT GCTAACGTCA GCTGGAAGCA CAACTTCTCC
    GTTGACAGCA GCAAGTTTGT TGTAGAGTTC ACACTGGACA GTAATGGGTC GATGACGAGC
    ATTCAAGTAA ACCAGCAGCC TCACATTAAG CTGGCTGGGC TGGTGGCTGG GGCCAAGTAT
    CGGTTGCGGG TCTATTCGCA TGAGCACAAC TCCATCAGCA GCGACTATGT CACGTTCGAG
    ACAGGCAGAG CCTATAAGGA GCAGGAAGAC ATCGCTACTC AGGGCTGGTT CATTGGGTTG
    ATGTGTGCCG TCGCTTTATT GGTTCTGATT CTGCTCATTG TTTGTTTCAT CAAGAGGAGT
    CGTGGAGGAA AATACCCAGT GAGAGAGAAG AAAGACCTCC CCTTAGATCC CGTCGATCAT
    AAAGACCAAG ATGGATCATT TGACTATCAC AGCGACGAAG ACAACAAGCC GTTACAGGGC
    AGCCAAACGT CCCTGGACGG GAACGTTAAA GAAAGCGACG ACAGTCTCGT GGACTACGGC
    GAAGGAGGAG ACGGACAATT CAACGAGGAT GGTTCCTTCA TCGGCCAATA CACAGTAAAG
    AAGGACAAAG AGGAGACAGA AGGAAATGAG AGCTCAGAAG CCACATCACC TGTCAATGCC
    ATCTACTCTT TGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213671.1
    CDS298..4455
    Translation

    Target ORF information:

    RefSeq Version XM_017358182.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358182.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGAGAATGAC AGCTCGTCTG CTTCAAATCG TAGTGCCTCT
    GCAGGTCGAC CCGACCCACC TCAGGATCTG GAACTCTCTG ACCCTTCAGC ACGCAGTGTC
    AGACTCACCT GGGTGCCCGG AAATGAAAAC AACAGTCCTG TTACACATTT CCTAGTCCAG
    TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG TGGCAGAACC TGTCAATTTA TGATTGGAAC
    CTAAACTCAG TTAACCTTCA GCTATCACCA TTCGTCAACT ACCAGTTCAG AGTGATTGCG
    ATCAATGCTG TGGGTCAGAG TCAACCCAGC CACCCCTCTG CACGTTATCA GACCAGCGGA
    GCCTCTCCTG ATGTTGTACC AAGTGGACTT AAAGGGTGGG GAAGCAAAAA AAACAACATG
    GAGATCACAT GGCAGGCCAT GCAGAACACA GAGAGGAATG GGCCAAACCT GCGATATGTG
    CTCTCCTGGA GAAGAAAAGA CACAGAAGAA GAGTGGAATA ACATCACCAC AGCCAAAACA
    AAACACGTTG TCCATAATAC AGACACCTAC GTTCCTTATG AGATCAAAAT ACAGGCTGTC
    AATGACTTTG GACATGGCCC AGAGTCAAAC ATGGTCATCG GTTATTCTGG GGAAGACAAA
    CCATCCGCTG CACCAACAGA TCTTAGGGTG TCAAAGATCA ACAGTACCAA AGTCAATATT
    CACTGGGAGC CTGTGGAGCA GAGCAGTGTA AATGGAGAAT TTAAAGAATA CAAGTTGTAC
    TACTGGCGGG AGGCCAGCCT CATAAAGGGC CTGAAAGTAA ATAAGACAAA GTTGCATAAG
    GGATTCTTTA CCACAGCAGA ACATCCCTCT GGCATCCTGA CAGAGCTCTA TCCATACAGC
    AAATATAAGA TGTATATGGT GGTTGCTAAC CGAGAACATG AAGGGCCGCA AAGCAATACT
    GTGGAGTTTT ACACCAAGGA GGGAGAGCCA GGGGCTCCAA AATACTTTAG GATTCTAAGA
    CACACGGACG TGGTTCACCT GAAGTGGGAC AAACCACTGG AGCCAAACGG TGTCCTGATT
    GGATACACAC TCCAGTACCA CACAGTGAAC GGGACACAGG CGGGAGAGAG GAAAGTCCAG
    AGTTTCCCAC CCAATGTGAC GGAGTACGTC ATGAATCTGC CTCACCGCTT CACGCGCTTC
    AAGTTCTTCC TAGCAGCCCG TACGCAGGTC GGAGCAGGAG AAGTGTACGC TGTCGAGTCT
    CCACACTTCA CCAATGAAGG CACCCCAGAT ACTGACTCTA CAGTTGTAGC CGTTAACTTT
    ACAGATGGCT TGATGTTCGA AATTCCTCCT CCTCCCCTTC CCCCCTCCAC TGACCCAAAC
    CCAACAATAT TCACCCCAGA ACCAACTCCA CCAGTCCCTA CAACTGCTGC TCCAACCAAA
    CCTACTACCA CCACCACCAC CACCACTACT ACTACTACAA CTTCTACTAC TACTACAACT
    ACCACAACTA CTACAACAAC CACCACTACT ACAGCAGCTT CCACCACAGT TGCTCCAGTT
    CGGCCCGTGC CTCCCAAAAC AGTGGATTGG AAAATGCTGG CCCCGAATGT AAAGATCTGG
    AATCTGACGG TGGATGCTAA CAATGACAAT GCTAACGTCA GCTGGAAGCA CAACTTCTCC
    GTTGACAGCA GCAAGTTTGT TGTAGAGTTC ACACTGGACA GTAATGGGTC GATGACGAGC
    ATTCAAGTAA ACCAGCAGCC TCACATTAAG CTGGCTGGGC TGGTGGCTGG GGCCAAGTAT
    CGGTTGCGGG TCTATTCGCA TGAGCACAAC TCCATCAGCA GCGACTATGT CACGTTCGAG
    ACAGGCAGAG CCTATAAGGA GCAGGAAGAC ATCGCTACTC AGGGCTGGTT CATTGGGTTG
    ATGTGTGCCG TCGCTTTATT GGTTCTGATT CTGCTCATTG TTTGTTTCAT CAAGAGGAGT
    CGTGGAGGAA AATACCCAGT GAGAGAGAAG AAAGACCTCC CCTTAGATCC CGTCGATCAT
    AAAGACCAAG ATGGATCATT TGACTATCAC AGCGACGAAG ACAACAAGCC GTTACAGGGC
    AGCCAAACGT CCCTGGACGG GAACGTTAAA GAAAGCGACG ACAGTCTCGT GGACTACGGC
    GAAGGAGGAG ACGGACAATT CAACGAGGAT GGTTCCTTCA TCGGCCAATA CACAGTAAAG
    AAGGACAAAG AGGAGACAGA AGGAAATGAG AGCTCAGAAG CCACATCACC TGTCAATGCC
    ATCTACTCTT TGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54238
    Clone ID Related Accession (Same CDS sequence) XM_017358182.1 , XM_017358183.1
    Accession Version XM_017358183.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofascin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGAGAATGAC AGCTCGTCTG CTTCAAATCG TAGTGCCTCT
    GCAGGTCGAC CCGACCCACC TCAGGATCTG GAACTCTCTG ACCCTTCAGC ACGCAGTGTC
    AGACTCACCT GGGTGCCCGG AAATGAAAAC AACAGTCCTG TTACACATTT CCTAGTCCAG
    TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG TGGCAGAACC TGTCAATTTA TGATTGGAAC
    CTAAACTCAG TTAACCTTCA GCTATCACCA TTCGTCAACT ACCAGTTCAG AGTGATTGCG
    ATCAATGCTG TGGGTCAGAG TCAACCCAGC CACCCCTCTG CACGTTATCA GACCAGCGGA
    GCCTCTCCTG ATGTTGTACC AAGTGGACTT AAAGGGTGGG GAAGCAAAAA AAACAACATG
    GAGATCACAT GGCAGGCCAT GCAGAACACA GAGAGGAATG GGCCAAACCT GCGATATGTG
    CTCTCCTGGA GAAGAAAAGA CACAGAAGAA GAGTGGAATA ACATCACCAC AGCCAAAACA
    AAACACGTTG TCCATAATAC AGACACCTAC GTTCCTTATG AGATCAAAAT ACAGGCTGTC
    AATGACTTTG GACATGGCCC AGAGTCAAAC ATGGTCATCG GTTATTCTGG GGAAGACAAA
    CCATCCGCTG CACCAACAGA TCTTAGGGTG TCAAAGATCA ACAGTACCAA AGTCAATATT
    CACTGGGAGC CTGTGGAGCA GAGCAGTGTA AATGGAGAAT TTAAAGAATA CAAGTTGTAC
    TACTGGCGGG AGGCCAGCCT CATAAAGGGC CTGAAAGTAA ATAAGACAAA GTTGCATAAG
    GGATTCTTTA CCACAGCAGA ACATCCCTCT GGCATCCTGA CAGAGCTCTA TCCATACAGC
    AAATATAAGA TGTATATGGT GGTTGCTAAC CGAGAACATG AAGGGCCGCA AAGCAATACT
    GTGGAGTTTT ACACCAAGGA GGGAGAGCCA GGGGCTCCAA AATACTTTAG GATTCTAAGA
    CACACGGACG TGGTTCACCT GAAGTGGGAC AAACCACTGG AGCCAAACGG TGTCCTGATT
    GGATACACAC TCCAGTACCA CACAGTGAAC GGGACACAGG CGGGAGAGAG GAAAGTCCAG
    AGTTTCCCAC CCAATGTGAC GGAGTACGTC ATGAATCTGC CTCACCGCTT CACGCGCTTC
    AAGTTCTTCC TAGCAGCCCG TACGCAGGTC GGAGCAGGAG AAGTGTACGC TGTCGAGTCT
    CCACACTTCA CCAATGAAGG CACCCCAGAT ACTGACTCTA CAGTTGTAGC CGTTAACTTT
    ACAGATGGCT TGATGTTCGA AATTCCTCCT CCTCCCCTTC CCCCCTCCAC TGACCCAAAC
    CCAACAATAT TCACCCCAGA ACCAACTCCA CCAGTCCCTA CAACTGCTGC TCCAACCAAA
    CCTACTACCA CCACCACCAC CACCACTACT ACTACTACAA CTTCTACTAC TACTACAACT
    ACCACAACTA CTACAACAAC CACCACTACT ACAGCAGCTT CCACCACAGT TGCTCCAGTT
    CGGCCCGTGC CTCCCAAAAC AGTGGATTGG AAAATGCTGG CCCCGAATGT AAAGATCTGG
    AATCTGACGG TGGATGCTAA CAATGACAAT GCTAACGTCA GCTGGAAGCA CAACTTCTCC
    GTTGACAGCA GCAAGTTTGT TGTAGAGTTC ACACTGGACA GTAATGGGTC GATGACGAGC
    ATTCAAGTAA ACCAGCAGCC TCACATTAAG CTGGCTGGGC TGGTGGCTGG GGCCAAGTAT
    CGGTTGCGGG TCTATTCGCA TGAGCACAAC TCCATCAGCA GCGACTATGT CACGTTCGAG
    ACAGGCAGAG CCTATAAGGA GCAGGAAGAC ATCGCTACTC AGGGCTGGTT CATTGGGTTG
    ATGTGTGCCG TCGCTTTATT GGTTCTGATT CTGCTCATTG TTTGTTTCAT CAAGAGGAGT
    CGTGGAGGAA AATACCCAGT GAGAGAGAAG AAAGACCTCC CCTTAGATCC CGTCGATCAT
    AAAGACCAAG ATGGATCATT TGACTATCAC AGCGACGAAG ACAACAAGCC GTTACAGGGC
    AGCCAAACGT CCCTGGACGG GAACGTTAAA GAAAGCGACG ACAGTCTCGT GGACTACGGC
    GAAGGAGGAG ACGGACAATT CAACGAGGAT GGTTCCTTCA TCGGCCAATA CACAGTAAAG
    AAGGACAAAG AGGAGACAGA AGGAAATGAG AGCTCAGAAG CCACATCACC TGTCAATGCC
    ATCTACTCTT TGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213672.1
    CDS593..4750
    Translation

    Target ORF information:

    RefSeq Version XM_017358183.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358183.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGAGAATGAC AGCTCGTCTG CTTCAAATCG TAGTGCCTCT
    GCAGGTCGAC CCGACCCACC TCAGGATCTG GAACTCTCTG ACCCTTCAGC ACGCAGTGTC
    AGACTCACCT GGGTGCCCGG AAATGAAAAC AACAGTCCTG TTACACATTT CCTAGTCCAG
    TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG TGGCAGAACC TGTCAATTTA TGATTGGAAC
    CTAAACTCAG TTAACCTTCA GCTATCACCA TTCGTCAACT ACCAGTTCAG AGTGATTGCG
    ATCAATGCTG TGGGTCAGAG TCAACCCAGC CACCCCTCTG CACGTTATCA GACCAGCGGA
    GCCTCTCCTG ATGTTGTACC AAGTGGACTT AAAGGGTGGG GAAGCAAAAA AAACAACATG
    GAGATCACAT GGCAGGCCAT GCAGAACACA GAGAGGAATG GGCCAAACCT GCGATATGTG
    CTCTCCTGGA GAAGAAAAGA CACAGAAGAA GAGTGGAATA ACATCACCAC AGCCAAAACA
    AAACACGTTG TCCATAATAC AGACACCTAC GTTCCTTATG AGATCAAAAT ACAGGCTGTC
    AATGACTTTG GACATGGCCC AGAGTCAAAC ATGGTCATCG GTTATTCTGG GGAAGACAAA
    CCATCCGCTG CACCAACAGA TCTTAGGGTG TCAAAGATCA ACAGTACCAA AGTCAATATT
    CACTGGGAGC CTGTGGAGCA GAGCAGTGTA AATGGAGAAT TTAAAGAATA CAAGTTGTAC
    TACTGGCGGG AGGCCAGCCT CATAAAGGGC CTGAAAGTAA ATAAGACAAA GTTGCATAAG
    GGATTCTTTA CCACAGCAGA ACATCCCTCT GGCATCCTGA CAGAGCTCTA TCCATACAGC
    AAATATAAGA TGTATATGGT GGTTGCTAAC CGAGAACATG AAGGGCCGCA AAGCAATACT
    GTGGAGTTTT ACACCAAGGA GGGAGAGCCA GGGGCTCCAA AATACTTTAG GATTCTAAGA
    CACACGGACG TGGTTCACCT GAAGTGGGAC AAACCACTGG AGCCAAACGG TGTCCTGATT
    GGATACACAC TCCAGTACCA CACAGTGAAC GGGACACAGG CGGGAGAGAG GAAAGTCCAG
    AGTTTCCCAC CCAATGTGAC GGAGTACGTC ATGAATCTGC CTCACCGCTT CACGCGCTTC
    AAGTTCTTCC TAGCAGCCCG TACGCAGGTC GGAGCAGGAG AAGTGTACGC TGTCGAGTCT
    CCACACTTCA CCAATGAAGG CACCCCAGAT ACTGACTCTA CAGTTGTAGC CGTTAACTTT
    ACAGATGGCT TGATGTTCGA AATTCCTCCT CCTCCCCTTC CCCCCTCCAC TGACCCAAAC
    CCAACAATAT TCACCCCAGA ACCAACTCCA CCAGTCCCTA CAACTGCTGC TCCAACCAAA
    CCTACTACCA CCACCACCAC CACCACTACT ACTACTACAA CTTCTACTAC TACTACAACT
    ACCACAACTA CTACAACAAC CACCACTACT ACAGCAGCTT CCACCACAGT TGCTCCAGTT
    CGGCCCGTGC CTCCCAAAAC AGTGGATTGG AAAATGCTGG CCCCGAATGT AAAGATCTGG
    AATCTGACGG TGGATGCTAA CAATGACAAT GCTAACGTCA GCTGGAAGCA CAACTTCTCC
    GTTGACAGCA GCAAGTTTGT TGTAGAGTTC ACACTGGACA GTAATGGGTC GATGACGAGC
    ATTCAAGTAA ACCAGCAGCC TCACATTAAG CTGGCTGGGC TGGTGGCTGG GGCCAAGTAT
    CGGTTGCGGG TCTATTCGCA TGAGCACAAC TCCATCAGCA GCGACTATGT CACGTTCGAG
    ACAGGCAGAG CCTATAAGGA GCAGGAAGAC ATCGCTACTC AGGGCTGGTT CATTGGGTTG
    ATGTGTGCCG TCGCTTTATT GGTTCTGATT CTGCTCATTG TTTGTTTCAT CAAGAGGAGT
    CGTGGAGGAA AATACCCAGT GAGAGAGAAG AAAGACCTCC CCTTAGATCC CGTCGATCAT
    AAAGACCAAG ATGGATCATT TGACTATCAC AGCGACGAAG ACAACAAGCC GTTACAGGGC
    AGCCAAACGT CCCTGGACGG GAACGTTAAA GAAAGCGACG ACAGTCTCGT GGACTACGGC
    GAAGGAGGAG ACGGACAATT CAACGAGGAT GGTTCCTTCA TCGGCCAATA CACAGTAAAG
    AAGGACAAAG AGGAGACAGA AGGAAATGAG AGCTCAGAAG CCACATCACC TGTCAATGCC
    ATCTACTCTT TGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54239
    Clone ID Related Accession (Same CDS sequence) XM_017358184.1
    Accession Version XM_017358184.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofascin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCTGAAAC AGCCCCCCAC CATTGTGAAG
    CAGTCACTAA AGGACTACAT TGTGGACCCC AGAGATAACA TCATCATTGA ATGCGAGGCC
    AAGGGGAACC CAGTGCCAAC GTTTCAGTGG AGGCGGAATG GAAAGTTCTT TAATGTGGGT
    AAGGACCCAC GGGTGACCAT GAGAAGTCGA TCTGGAACAC TAGAGATCAG AAGCAGTGGA
    AAACCAGAAG ATTATGAAGG AGAATACCAG TGCTTCGCTT CCAACAACTT GGGCACAGCC
    ATTTCCAACA AAATCCTGCT TCGAGTGTCA AAGTCTCCGC TGTGGCCAAA GGAGGTTTTG
    GAACCGGTAA CTGTGCGTGA GGGATCACCG CTCGTCCTGG CCTGCAACCC TCCTCCTGGT
    CTTCCCCCTC CTGAAACCTT CTGGATGGAC AGCTTCATTA TGCCTATAGC ACAGGACAGG
    AGGGTGTCAA TGGGCCTGAA CGGTGACTTG TTCTTCTCCA ACGTTCTAGC TAAAGATGCC
    AAGACTGACT ACAGCTGCAA TGCTCGCTTC GAGTTCACAC ACACCATACA GCAGAAGAAC
    CCCTACACAC TTAAAGTGCA AACAAAAGAA CCTTATAATG ACACCTTTCT CAACTCAACT
    GATGTGTATG GTGTGCGTAA TATACCGGAA ACCCAGCCCA CCTTTCTATC TCCAACGGGC
    TTGTCCAGCA CCAAGATGGT GCTCAGAGGA GAAGAACTTC TCCTGGAGTG TATAGCAGCT
    GGAGTACCAA CTCCTACCAT TACCTGGTCC AAAAGAGGAG GAGACCTGCC AACGAAAAAA
    GTCAGGATTG GGAACTTCGA TAAAACCTTA CGCATTCTCA ATGTGTCTGA GGAAGACATT
    GGCAGCTACA CCTGCATGGC CAGCAACAGA ATTGGCAGCA TTCGCCACAC AGTTGAAGTC
    CAGGTCAAAG CTGCTCCATT TTGGCTCGAA AAGCCCATCA ATCTGGTTCT TGCCCCTGAT
    GAGAGTGGAC GGTTAGTGTG CCGTGCTAAC GGGAGTCCCA AACCCACTAT TCAATGGCTG
    GTGAATGGAG AACCCATTGA AAGTTCACTC CCCAACCCCA ATCGGAGAGT TCTTGATGAC
    ACGATCATAT TTGAGACGGT TCAGTTTGAA AGCAGTGCAG TTTACCAGTG TAACGCCTCC
    AATGAGCACG GTTATCTTCT GGCAAATGCT TTCGTCAGTG TTTTGGATAT GGCACCGCGG
    ATGCTTGGCC CGAAAAACCA GCTGATCAAA GTCATTGAAA ACAACAGGAC CTTCCTCGAG
    TGTCCTTTCT TTGGCTCACC GCTCCCTTTA CTTCGCTGGT TTAAGAACGG ACTTGGCAGT
    GGTTTGGATG GTGGTCACTA CAGGCTATAC CACAATGGCA CTTTGGAGAT CAAGCAGGCC
    CGGACAGAAG ACCAGGGCAC CTACACCTGC GTCGCCAGCA ACATTCTGGG CAAGATGGAG
    AATCAAGTGC GCCTAGAGGT TAAAGAGCCC ACAAGAATAG TCCGCGCACC TGAACATAAG
    ACGGAACCCA GAGGAACCAC AGTTCGATTC GACTGTCGAG TTAAACATGA TCCTTCCCTT
    CCTGCTACAG TCACATGGCT GAAAGATGAA AAGCCTCTGA GCTTTAGCTG GATTGGCCGG
    TTGAAAAAGG ATGATGAGTC CTTGACTATT TACAATGTCA ATCCAGACGA TGGAGGAACC
    TACACATGCA CAGCCAAAAC TGAGATAGAC GAGGACACTG CCTCAGCTCG CCTTACTGTT
    ACAGAGAATG ACAGCTCGTC TGCTTCAAAT CGTAGTGCCT CTGCAGGTCG ACCCGACCCA
    CCTCAGGATC TGGAACTCTC TGACCCTTCA GCACGCAGTG TCAGACTCAC CTGGGTGCCC
    GGAAATGAAA ACAACAGTCC TGTTACACAT TTCCTAGTCC AGTTTGAAGA GGACAGGTGG
    GAGCCAGGGA AGTGGCAGAA CCTGTCAATT TATGATTGGA ACCTAAACTC AGTTAACCTT
    CAGCTATCAC CATTCGTCAA CTACCAGTTC AGAGTGATTG CGATCAATGC TGTGGGTCAG
    AGTCAACCCA GCCACCCCTC TGCACGTTAT CAGACCAGCG GAGCCTCTCC TGATGTTGTA
    CCAAGTGGAC TTAAAGGGTG GGGAAGCAAA AAAAACAACA TGGAGATCAC ATGGCAGGCC
    ATGCAGAACA CAGAGAGGAA TGGGCCAAAC CTGCGATATG TGCTCTCCTG GAGAAGAAAA
    GACACAGAAG AAGAGTGGAA TAACATCACC ACAGCCAAAA CAAAACACGT TGTCCATAAT
    ACAGACACCT ACGTTCCTTA TGAGATCAAA ATACAGGCTG TCAATGACTT TGGACATGGC
    CCAGAGTCAA ACATGGTCAT CGGTTATTCT GGGGAAGACA AACCATCCGC TGCACCAACA
    GATCTTAGGG TGTCAAAGAT CAACAGTACC AAAGTCAATA TTCACTGGGA GCCTGTGGAG
    CAGAGCAGTG TAAATGGAGA ATTTAAAGAA TACAAGTTGT ACTACTGGCG GGAGGCCAGC
    CTCATAAAGG GCCTGAAAGT AAATAAGACA AAGTTGCATA AGGGATTCTT TACCACAGCA
    GAACATCCCT CTGGCATCCT GACAGAGCTC TATCCATACA GCAAATATAA GATGTATATG
    GTGGTTGCTA ACCGAGAACA TGAAGGGCCG CAAAGCAATA CTGTGGAGTT TTACACCAAG
    GAGGGAGAGC CAGGGGCTCC AAAATACTTT AGGATTCTAA GACACACGGA CGTGGTTCAC
    CTGAAGTGGG ACAAACCACT GGAGCCAAAC GGTGTCCTGA TTGGATACAC ACTCCAGTAC
    CACACAGTGA ACGGGACACA GGCGGGAGAG AGGAAAGTCC AGAGTTTCCC ACCCAATGTG
    ACGGAGTACG TCATGAATCT GCCTCACCGC TTCACGCGCT TCAAGTTCTT CCTAGCAGCC
    CGTACGCAGG TCGGAGCAGG AGAAGTGTAC GCTGTCGAGT CTCCACACTT CACCAATGAA
    GGCACCCCAG ATACTGACTC TACAGTTGTA GCCGTTAACT TTACAGATGG CTTGATGTTC
    GAAATTCCTC CTCCTCCCCT TCCCCCCTCC ACTGACCCAA ACCCAACAAT ATTCACCCCA
    GAACCAACTC CACCAGTCCC TACAACTGCT GCTCCAACCA AACCTACTAC CACCACCACC
    ACCACCACTA CTACTACTAC AACTTCTACT ACTACTACAA CTACCACAAC TACTACAACA
    ACCACCACTA CTACAGCAGC TTCCACCACA GTTGCTCCAG TTCGGCCCGT GCCTCCCAAA
    ACAGTGGATT GGAAAATGCT GGCCCCGAAT GTAAAGATCT GGAATCTGAC GGTGGATGCT
    AACAATGACA ATGCTAACGT CAGCTGGAAG CACAACTTCT CCGTTGACAG CAGCAAGTTT
    GTTGTAGAGT TCACACTGGA CAGTAATGGG TCGATGACGA GCATTCAAGT AAACCAGCAG
    CCTCACATTA AGCTGGCTGG GCTGGTGGCT GGGGCCAAGT ATCGGTTGCG GGTCTATTCG
    CATGAGCACA ACTCCATCAG CAGCGACTAT GTCACGTTCG AGACAGGCAG AGCCTATAAG
    GAGCAGGAAG ACATCGCTAC TCAGGGCTGG TTCATTGGGT TGATGTGTGC CGTCGCTTTA
    TTGGTTCTGA TTCTGCTCAT TGTTTGTTTC ATCAAGAGGA GTCGTGGAGG AAAATACCCA
    GTGAGAGAGA AGAAAGACCT CCCCTTAGAT CCCGTCGATC ATAAAGACCA AGATGGATCA
    TTTGACTATC ACAGCGACGA AGACAACAAG CCGTTACAGG GCAGCCAAAC GTCCCTGGAC
    GGGAACGTTA AAGAAAGCGA CGACAGTCTC GTGGACTACG GCGAAGGAGG AGACGGACAA
    TTCAACGAGG ATGGTTCCTT CATCGGCCAA TACACAGTAA AGAAGGACAA AGAGGAGACA
    GAAGGAAATG AGAGCTCAGA AGCCACATCA CCTGTCAATG CCATCTACTC TTTGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213673.1
    CDS298..4437
    Translation

    Target ORF information:

    RefSeq Version XM_017358184.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCTGAAAC AGCCCCCCAC CATTGTGAAG
    CAGTCACTAA AGGACTACAT TGTGGACCCC AGAGATAACA TCATCATTGA ATGCGAGGCC
    AAGGGGAACC CAGTGCCAAC GTTTCAGTGG AGGCGGAATG GAAAGTTCTT TAATGTGGGT
    AAGGACCCAC GGGTGACCAT GAGAAGTCGA TCTGGAACAC TAGAGATCAG AAGCAGTGGA
    AAACCAGAAG ATTATGAAGG AGAATACCAG TGCTTCGCTT CCAACAACTT GGGCACAGCC
    ATTTCCAACA AAATCCTGCT TCGAGTGTCA AAGTCTCCGC TGTGGCCAAA GGAGGTTTTG
    GAACCGGTAA CTGTGCGTGA GGGATCACCG CTCGTCCTGG CCTGCAACCC TCCTCCTGGT
    CTTCCCCCTC CTGAAACCTT CTGGATGGAC AGCTTCATTA TGCCTATAGC ACAGGACAGG
    AGGGTGTCAA TGGGCCTGAA CGGTGACTTG TTCTTCTCCA ACGTTCTAGC TAAAGATGCC
    AAGACTGACT ACAGCTGCAA TGCTCGCTTC GAGTTCACAC ACACCATACA GCAGAAGAAC
    CCCTACACAC TTAAAGTGCA AACAAAAGAA CCTTATAATG ACACCTTTCT CAACTCAACT
    GATGTGTATG GTGTGCGTAA TATACCGGAA ACCCAGCCCA CCTTTCTATC TCCAACGGGC
    TTGTCCAGCA CCAAGATGGT GCTCAGAGGA GAAGAACTTC TCCTGGAGTG TATAGCAGCT
    GGAGTACCAA CTCCTACCAT TACCTGGTCC AAAAGAGGAG GAGACCTGCC AACGAAAAAA
    GTCAGGATTG GGAACTTCGA TAAAACCTTA CGCATTCTCA ATGTGTCTGA GGAAGACATT
    GGCAGCTACA CCTGCATGGC CAGCAACAGA ATTGGCAGCA TTCGCCACAC AGTTGAAGTC
    CAGGTCAAAG CTGCTCCATT TTGGCTCGAA AAGCCCATCA ATCTGGTTCT TGCCCCTGAT
    GAGAGTGGAC GGTTAGTGTG CCGTGCTAAC GGGAGTCCCA AACCCACTAT TCAATGGCTG
    GTGAATGGAG AACCCATTGA AAGTTCACTC CCCAACCCCA ATCGGAGAGT TCTTGATGAC
    ACGATCATAT TTGAGACGGT TCAGTTTGAA AGCAGTGCAG TTTACCAGTG TAACGCCTCC
    AATGAGCACG GTTATCTTCT GGCAAATGCT TTCGTCAGTG TTTTGGATAT GGCACCGCGG
    ATGCTTGGCC CGAAAAACCA GCTGATCAAA GTCATTGAAA ACAACAGGAC CTTCCTCGAG
    TGTCCTTTCT TTGGCTCACC GCTCCCTTTA CTTCGCTGGT TTAAGAACGG ACTTGGCAGT
    GGTTTGGATG GTGGTCACTA CAGGCTATAC CACAATGGCA CTTTGGAGAT CAAGCAGGCC
    CGGACAGAAG ACCAGGGCAC CTACACCTGC GTCGCCAGCA ACATTCTGGG CAAGATGGAG
    AATCAAGTGC GCCTAGAGGT TAAAGAGCCC ACAAGAATAG TCCGCGCACC TGAACATAAG
    ACGGAACCCA GAGGAACCAC AGTTCGATTC GACTGTCGAG TTAAACATGA TCCTTCCCTT
    CCTGCTACAG TCACATGGCT GAAAGATGAA AAGCCTCTGA GCTTTAGCTG GATTGGCCGG
    TTGAAAAAGG ATGATGAGTC CTTGACTATT TACAATGTCA ATCCAGACGA TGGAGGAACC
    TACACATGCA CAGCCAAAAC TGAGATAGAC GAGGACACTG CCTCAGCTCG CCTTACTGTT
    ACAGAGAATG ACAGCTCGTC TGCTTCAAAT CGTAGTGCCT CTGCAGGTCG ACCCGACCCA
    CCTCAGGATC TGGAACTCTC TGACCCTTCA GCACGCAGTG TCAGACTCAC CTGGGTGCCC
    GGAAATGAAA ACAACAGTCC TGTTACACAT TTCCTAGTCC AGTTTGAAGA GGACAGGTGG
    GAGCCAGGGA AGTGGCAGAA CCTGTCAATT TATGATTGGA ACCTAAACTC AGTTAACCTT
    CAGCTATCAC CATTCGTCAA CTACCAGTTC AGAGTGATTG CGATCAATGC TGTGGGTCAG
    AGTCAACCCA GCCACCCCTC TGCACGTTAT CAGACCAGCG GAGCCTCTCC TGATGTTGTA
    CCAAGTGGAC TTAAAGGGTG GGGAAGCAAA AAAAACAACA TGGAGATCAC ATGGCAGGCC
    ATGCAGAACA CAGAGAGGAA TGGGCCAAAC CTGCGATATG TGCTCTCCTG GAGAAGAAAA
    GACACAGAAG AAGAGTGGAA TAACATCACC ACAGCCAAAA CAAAACACGT TGTCCATAAT
    ACAGACACCT ACGTTCCTTA TGAGATCAAA ATACAGGCTG TCAATGACTT TGGACATGGC
    CCAGAGTCAA ACATGGTCAT CGGTTATTCT GGGGAAGACA AACCATCCGC TGCACCAACA
    GATCTTAGGG TGTCAAAGAT CAACAGTACC AAAGTCAATA TTCACTGGGA GCCTGTGGAG
    CAGAGCAGTG TAAATGGAGA ATTTAAAGAA TACAAGTTGT ACTACTGGCG GGAGGCCAGC
    CTCATAAAGG GCCTGAAAGT AAATAAGACA AAGTTGCATA AGGGATTCTT TACCACAGCA
    GAACATCCCT CTGGCATCCT GACAGAGCTC TATCCATACA GCAAATATAA GATGTATATG
    GTGGTTGCTA ACCGAGAACA TGAAGGGCCG CAAAGCAATA CTGTGGAGTT TTACACCAAG
    GAGGGAGAGC CAGGGGCTCC AAAATACTTT AGGATTCTAA GACACACGGA CGTGGTTCAC
    CTGAAGTGGG ACAAACCACT GGAGCCAAAC GGTGTCCTGA TTGGATACAC ACTCCAGTAC
    CACACAGTGA ACGGGACACA GGCGGGAGAG AGGAAAGTCC AGAGTTTCCC ACCCAATGTG
    ACGGAGTACG TCATGAATCT GCCTCACCGC TTCACGCGCT TCAAGTTCTT CCTAGCAGCC
    CGTACGCAGG TCGGAGCAGG AGAAGTGTAC GCTGTCGAGT CTCCACACTT CACCAATGAA
    GGCACCCCAG ATACTGACTC TACAGTTGTA GCCGTTAACT TTACAGATGG CTTGATGTTC
    GAAATTCCTC CTCCTCCCCT TCCCCCCTCC ACTGACCCAA ACCCAACAAT ATTCACCCCA
    GAACCAACTC CACCAGTCCC TACAACTGCT GCTCCAACCA AACCTACTAC CACCACCACC
    ACCACCACTA CTACTACTAC AACTTCTACT ACTACTACAA CTACCACAAC TACTACAACA
    ACCACCACTA CTACAGCAGC TTCCACCACA GTTGCTCCAG TTCGGCCCGT GCCTCCCAAA
    ACAGTGGATT GGAAAATGCT GGCCCCGAAT GTAAAGATCT GGAATCTGAC GGTGGATGCT
    AACAATGACA ATGCTAACGT CAGCTGGAAG CACAACTTCT CCGTTGACAG CAGCAAGTTT
    GTTGTAGAGT TCACACTGGA CAGTAATGGG TCGATGACGA GCATTCAAGT AAACCAGCAG
    CCTCACATTA AGCTGGCTGG GCTGGTGGCT GGGGCCAAGT ATCGGTTGCG GGTCTATTCG
    CATGAGCACA ACTCCATCAG CAGCGACTAT GTCACGTTCG AGACAGGCAG AGCCTATAAG
    GAGCAGGAAG ACATCGCTAC TCAGGGCTGG TTCATTGGGT TGATGTGTGC CGTCGCTTTA
    TTGGTTCTGA TTCTGCTCAT TGTTTGTTTC ATCAAGAGGA GTCGTGGAGG AAAATACCCA
    GTGAGAGAGA AGAAAGACCT CCCCTTAGAT CCCGTCGATC ATAAAGACCA AGATGGATCA
    TTTGACTATC ACAGCGACGA AGACAACAAG CCGTTACAGG GCAGCCAAAC GTCCCTGGAC
    GGGAACGTTA AAGAAAGCGA CGACAGTCTC GTGGACTACG GCGAAGGAGG AGACGGACAA
    TTCAACGAGG ATGGTTCCTT CATCGGCCAA TACACAGTAA AGAAGGACAA AGAGGAGACA
    GAAGGAAATG AGAGCTCAGA AGCCACATCA CCTGTCAATG CCATCTACTC TTTGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54240
    Clone ID Related Accession (Same CDS sequence) XM_017358185.1
    Accession Version XM_017358185.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofascin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGGTCGACCC GACCCACCTC AGGATCTGGA ACTCTCTGAC
    CCTTCAGCAC GCAGTGTCAG ACTCACCTGG GTGCCCGGAA ATGAAAACAA CAGTCCTGTT
    ACACATTTCC TAGTCCAGTT TGAAGAGGAC AGGTGGGAGC CAGGGAAGTG GCAGAACCTG
    TCAATTTATG ATTGGAACCT AAACTCAGTT AACCTTCAGC TATCACCATT CGTCAACTAC
    CAGTTCAGAG TGATTGCGAT CAATGCTGTG GGTCAGAGTC AACCCAGCCA CCCCTCTGCA
    CGTTATCAGA CCAGCGGAGC CTCTCCTGAT GTTGTACCAA GTGGACTTAA AGGGTGGGGA
    AGCAAAAAAA ACAACATGGA GATCACATGG CAGGCCATGC AGAACACAGA GAGGAATGGG
    CCAAACCTGC GATATGTGCT CTCCTGGAGA AGAAAAGACA CAGAAGAAGA GTGGAATAAC
    ATCACCACAG CCAAAACAAA ACACGTTGTC CATAATACAG ACACCTACGT TCCTTATGAG
    ATCAAAATAC AGGCTGTCAA TGACTTTGGA CATGGCCCAG AGTCAAACAT GGTCATCGGT
    TATTCTGGGG AAGACAAACC ATCCGCTGCA CCAACAGATC TTAGGGTGTC AAAGATCAAC
    AGTACCAAAG TCAATATTCA CTGGGAGCCT GTGGAGCAGA GCAGTGTAAA TGGAGAATTT
    AAAGAATACA AGTTGTACTA CTGGCGGGAG GCCAGCCTCA TAAAGGGCCT GAAAGTAAAT
    AAGACAAAGT TGCATAAGGG ATTCTTTACC ACAGCAGAAC ATCCCTCTGG CATCCTGACA
    GAGCTCTATC CATACAGCAA ATATAAGATG TATATGGTGG TTGCTAACCG AGAACATGAA
    GGGCCGCAAA GCAATACTGT GGAGTTTTAC ACCAAGGAGG GAGAGCCAGG GGCTCCAAAA
    TACTTTAGGA TTCTAAGACA CACGGACGTG GTTCACCTGA AGTGGGACAA ACCACTGGAG
    CCAAACGGTG TCCTGATTGG ATACACACTC CAGTACCACA CAGTGAACGG GACACAGGCG
    GGAGAGAGGA AAGTCCAGAG TTTCCCACCC AATGTGACGG AGTACGTCAT GAATCTGCCT
    CACCGCTTCA CGCGCTTCAA GTTCTTCCTA GCAGCCCGTA CGCAGGTCGG AGCAGGAGAA
    GTGTACGCTG TCGAGTCTCC ACACTTCACC AATGAAGGCA CCCCAGATAC TGACTCTACA
    GTTGTAGCCG TTAACTTTAC AGATGGCTTG ATGTTCGAAA TTCCTCCTCC TCCCCTTCCC
    CCCTCCACTG ACCCAAACCC AACAATATTC ACCCCAGAAC CAACTCCACC AGTCCCTACA
    ACTGCTGCTC CAACCAAACC TACTACCACC ACCACCACCA CCACTACTAC TACTACAACT
    TCTACTACTA CTACAACTAC CACAACTACT ACAACAACCA CCACTACTAC AGCAGCTTCC
    ACCACAGTTG CTCCAGTTCG GCCCGTGCCT CCCAAAACAG TGGATTGGAA AATGCTGGCC
    CCGAATGTAA AGATCTGGAA TCTGACGGTG GATGCTAACA ATGACAATGC TAACGTCAGC
    TGGAAGCACA ACTTCTCCGT TGACAGCAGC AAGTTTGTTG TAGAGTTCAC ACTGGACAGT
    AATGGGTCGA TGACGAGCAT TCAAGTAAAC CAGCAGCCTC ACATTAAGCT GGCTGGGCTG
    GTGGCTGGGG CCAAGTATCG GTTGCGGGTC TATTCGCATG AGCACAACTC CATCAGCAGC
    GACTATGTCA CGTTCGAGAC AGGCAGAGCC TATAAGGAGC AGGAAGACAT CGCTACTCAG
    GGCTGGTTCA TTGGGTTGAT GTGTGCCGTC GCTTTATTGG TTCTGATTCT GCTCATTGTT
    TGTTTCATCA AGAGGAGTCG TGGAGGAAAA TACCCAGTGA GAGAGAAGAA AGACCTCCCC
    TTAGATCCCG TCGATCATAA AGACCAAGAT GGATCATTTG ACTATCACAG CGACGAAGAC
    AACAAGCCGT TACAGGGCAG CCAAACGTCC CTGGACGGGA ACGTTAAAGA AAGCGACGAC
    AGTCTCGTGG ACTACGGCGA AGGAGGAGAC GGACAATTCA ACGAGGATGG TTCCTTCATC
    GGCCAATACA CAGTAAAGAA GGACAAAGAG GAGACAGAAG GAAATGAGAG CTCAGAAGCC
    ACATCACCTG TCAATGCCAT CTACTCTTTG GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213674.1
    CDS298..4413
    Translation

    Target ORF information:

    RefSeq Version XM_017358185.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358185.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGGTCGACCC GACCCACCTC AGGATCTGGA ACTCTCTGAC
    CCTTCAGCAC GCAGTGTCAG ACTCACCTGG GTGCCCGGAA ATGAAAACAA CAGTCCTGTT
    ACACATTTCC TAGTCCAGTT TGAAGAGGAC AGGTGGGAGC CAGGGAAGTG GCAGAACCTG
    TCAATTTATG ATTGGAACCT AAACTCAGTT AACCTTCAGC TATCACCATT CGTCAACTAC
    CAGTTCAGAG TGATTGCGAT CAATGCTGTG GGTCAGAGTC AACCCAGCCA CCCCTCTGCA
    CGTTATCAGA CCAGCGGAGC CTCTCCTGAT GTTGTACCAA GTGGACTTAA AGGGTGGGGA
    AGCAAAAAAA ACAACATGGA GATCACATGG CAGGCCATGC AGAACACAGA GAGGAATGGG
    CCAAACCTGC GATATGTGCT CTCCTGGAGA AGAAAAGACA CAGAAGAAGA GTGGAATAAC
    ATCACCACAG CCAAAACAAA ACACGTTGTC CATAATACAG ACACCTACGT TCCTTATGAG
    ATCAAAATAC AGGCTGTCAA TGACTTTGGA CATGGCCCAG AGTCAAACAT GGTCATCGGT
    TATTCTGGGG AAGACAAACC ATCCGCTGCA CCAACAGATC TTAGGGTGTC AAAGATCAAC
    AGTACCAAAG TCAATATTCA CTGGGAGCCT GTGGAGCAGA GCAGTGTAAA TGGAGAATTT
    AAAGAATACA AGTTGTACTA CTGGCGGGAG GCCAGCCTCA TAAAGGGCCT GAAAGTAAAT
    AAGACAAAGT TGCATAAGGG ATTCTTTACC ACAGCAGAAC ATCCCTCTGG CATCCTGACA
    GAGCTCTATC CATACAGCAA ATATAAGATG TATATGGTGG TTGCTAACCG AGAACATGAA
    GGGCCGCAAA GCAATACTGT GGAGTTTTAC ACCAAGGAGG GAGAGCCAGG GGCTCCAAAA
    TACTTTAGGA TTCTAAGACA CACGGACGTG GTTCACCTGA AGTGGGACAA ACCACTGGAG
    CCAAACGGTG TCCTGATTGG ATACACACTC CAGTACCACA CAGTGAACGG GACACAGGCG
    GGAGAGAGGA AAGTCCAGAG TTTCCCACCC AATGTGACGG AGTACGTCAT GAATCTGCCT
    CACCGCTTCA CGCGCTTCAA GTTCTTCCTA GCAGCCCGTA CGCAGGTCGG AGCAGGAGAA
    GTGTACGCTG TCGAGTCTCC ACACTTCACC AATGAAGGCA CCCCAGATAC TGACTCTACA
    GTTGTAGCCG TTAACTTTAC AGATGGCTTG ATGTTCGAAA TTCCTCCTCC TCCCCTTCCC
    CCCTCCACTG ACCCAAACCC AACAATATTC ACCCCAGAAC CAACTCCACC AGTCCCTACA
    ACTGCTGCTC CAACCAAACC TACTACCACC ACCACCACCA CCACTACTAC TACTACAACT
    TCTACTACTA CTACAACTAC CACAACTACT ACAACAACCA CCACTACTAC AGCAGCTTCC
    ACCACAGTTG CTCCAGTTCG GCCCGTGCCT CCCAAAACAG TGGATTGGAA AATGCTGGCC
    CCGAATGTAA AGATCTGGAA TCTGACGGTG GATGCTAACA ATGACAATGC TAACGTCAGC
    TGGAAGCACA ACTTCTCCGT TGACAGCAGC AAGTTTGTTG TAGAGTTCAC ACTGGACAGT
    AATGGGTCGA TGACGAGCAT TCAAGTAAAC CAGCAGCCTC ACATTAAGCT GGCTGGGCTG
    GTGGCTGGGG CCAAGTATCG GTTGCGGGTC TATTCGCATG AGCACAACTC CATCAGCAGC
    GACTATGTCA CGTTCGAGAC AGGCAGAGCC TATAAGGAGC AGGAAGACAT CGCTACTCAG
    GGCTGGTTCA TTGGGTTGAT GTGTGCCGTC GCTTTATTGG TTCTGATTCT GCTCATTGTT
    TGTTTCATCA AGAGGAGTCG TGGAGGAAAA TACCCAGTGA GAGAGAAGAA AGACCTCCCC
    TTAGATCCCG TCGATCATAA AGACCAAGAT GGATCATTTG ACTATCACAG CGACGAAGAC
    AACAAGCCGT TACAGGGCAG CCAAACGTCC CTGGACGGGA ACGTTAAAGA AAGCGACGAC
    AGTCTCGTGG ACTACGGCGA AGGAGGAGAC GGACAATTCA ACGAGGATGG TTCCTTCATC
    GGCCAATACA CAGTAAAGAA GGACAAAGAG GAGACAGAAG GAAATGAGAG CTCAGAAGCC
    ACATCACCTG TCAATGCCAT CTACTCTTTG GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54241
    Clone ID Related Accession (Same CDS sequence) XM_017358186.1
    Accession Version XM_017358186.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofascin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAATGCGTAA TATACCGGAA
    ACCCAGCCCA CCTTTCTATC TCCAACGGGC TTGTCCAGCA CCAAGATGGT GCTCAGAGGA
    GAAGAACTTC TCCTGGAGTG TATAGCAGCT GGAGTACCAA CTCCTACCAT TACCTGGTCC
    AAAAGAGGAG GAGACCTGCC AACGAAAAAA GTCAGGATTG GGAACTTCGA TAAAACCTTA
    CGCATTCTCA ATGTGTCTGA GGAAGACATT GGCAGCTACA CCTGCATGGC CAGCAACAGA
    ATTGGCAGCA TTCGCCACAC AGTTGAAGTC CAGGTCAAAG CTGCTCCATT TTGGCTCGAA
    AAGCCCATCA ATCTGGTTCT TGCCCCTGAT GAGAGTGGAC GGTTAGTGTG CCGTGCTAAC
    GGGAGTCCCA AACCCACTAT TCAATGGCTG GTGAATGGAG AACCCATTGA AAGTTCACTC
    CCCAACCCCA ATCGGAGAGT TCTTGATGAC ACGATCATAT TTGAGACGGT TCAGTTTGAA
    AGCAGTGCAG TTTACCAGTG TAACGCCTCC AATGAGCACG GTTATCTTCT GGCAAATGCT
    TTCGTCAGTG TTTTGGATAT GGCACCGCGG ATGCTTGGCC CGAAAAACCA GCTGATCAAA
    GTCATTGAAA ACAACAGGAC CTTCCTCGAG TGTCCTTTCT TTGGCTCACC GCTCCCTTTA
    CTTCGCTGGT TTAAGAACGG ACTTGGCAGT GGTTTGGATG GTGGTCACTA CAGGCTATAC
    CACAATGGCA CTTTGGAGAT CAAGCAGGCC CGGACAGAAG ACCAGGGCAC CTACACCTGC
    GTCGCCAGCA ACATTCTGGG CAAGATGGAG AATCAAGTGC GCCTAGAGGT TAAAGAGCCC
    ACAAGAATAG TCCGCGCACC TGAACATAAG ACGGAACCCA GAGGAACCAC AGTTCGATTC
    GACTGTCGAG TTAAACATGA TCCTTCCCTT CCTGCTACAG TCACATGGCT GAAAGATGAA
    AAGCCTCTGA GCTTTAGCTG GATTGGCCGG TTGAAAAAGG ATGATGAGTC CTTGACTATT
    TACAATGTCA ATCCAGACGA TGGAGGAACC TACACATGCA CAGCCAAAAC TGAGATAGAC
    GAGGACACTG CCTCAGCTCG CCTTACTGTT ACAGGTCGAC CCGACCCACC TCAGGATCTG
    GAACTCTCTG ACCCTTCAGC ACGCAGTGTC AGACTCACCT GGGTGCCCGG AAATGAAAAC
    AACAGTCCTG TTACACATTT CCTAGTCCAG TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG
    TGGCAGAACC TGTCAATTTA TGATTGGAAC CTAAACTCAG TTAACCTTCA GCTATCACCA
    TTCGTCAACT ACCAGTTCAG AGTGATTGCG ATCAATGCTG TGGGTCAGAG TCAACCCAGC
    CACCCCTCTG CACGTTATCA GACCAGCGGA GCCTCTCCTG ATGTTGTACC AAGTGGACTT
    AAAGGGTGGG GAAGCAAAAA AAACAACATG GAGATCACAT GGCAGGCCAT GCAGAACACA
    GAGAGGAATG GGCCAAACCT GCGATATGTG CTCTCCTGGA GAAGAAAAGA CACAGAAGAA
    GAGTGGAATA ACATCACCAC AGCCAAAACA AAACACGTTG TCCATAATAC AGACACCTAC
    GTTCCTTATG AGATCAAAAT ACAGGCTGTC AATGACTTTG GACATGGCCC AGAGTCAAAC
    ATGGTCATCG GTTATTCTGG GGAAGACAAA CCATCCGCTG CACCAACAGA TCTTAGGGTG
    TCAAAGATCA ACAGTACCAA AGTCAATATT CACTGGGAGC CTGTGGAGCA GAGCAGTGTA
    AATGGAGAAT TTAAAGAATA CAAGTTGTAC TACTGGCGGG AGGCCAGCCT CATAAAGGGC
    CTGAAAGTAA ATAAGACAAA GTTGCATAAG GGATTCTTTA CCACAGCAGA ACATCCCTCT
    GGCATCCTGA CAGAGCTCTA TCCATACAGC AAATATAAGA TGTATATGGT GGTTGCTAAC
    CGAGAACATG AAGGGCCGCA AAGCAATACT GTGGAGTTTT ACACCAAGGA GGGAGAGCCA
    GGGGCTCCAA AATACTTTAG GATTCTAAGA CACACGGACG TGGTTCACCT GAAGTGGGAC
    AAACCACTGG AGCCAAACGG TGTCCTGATT GGATACACAC TCCAGTACCA CACAGTGAAC
    GGGACACAGG CGGGAGAGAG GAAAGTCCAG AGTTTCCCAC CCAATGTGAC GGAGTACGTC
    ATGAATCTGC CTCACCGCTT CACGCGCTTC AAGTTCTTCC TAGCAGCCCG TACGCAGGTC
    GGAGCAGGAG AAGTGTACGC TGTCGAGTCT CCACACTTCA CCAATGAAGG CACCCCAGAT
    ACTGACTCTA CAGTTGTAGC CGTTAACTTT ACAGATGGCT TGATGTTCGA AATTCCTCCT
    CCTCCCCTTC CCCCCTCCAC TGACCCAAAC CCAACAATAT TCACCCCAGA ACCAACTCCA
    CCAGTCCCTA CAACTGCTGC TCCAACCAAA CCTACTACCA CCACCACCAC CACCACTACT
    ACTACTACAA CTTCTACTAC TACTACAACT ACCACAACTA CTACAACAAC CACCACTACT
    ACAGCAGCTT CCACCACAGT TGCTCCAGTT CGGCCCGTGC CTCCCAAAAC AGTGGATTGG
    AAAATGCTGG CCCCGAATGT AAAGATCTGG AATCTGACGG TGGATGCTAA CAATGACAAT
    GCTAACGTCA GCTGGAAGCA CAACTTCTCC GTTGACAGCA GCAAGTTTGT TGTAGAGTTC
    ACACTGGACA GTAATGGGTC GATGACGAGC ATTCAAGTAA ACCAGCAGCC TCACATTAAG
    CTGGCTGGGC TGGTGGCTGG GGCCAAGTAT CGGTTGCGGG TCTATTCGCA TGAGCACAAC
    TCCATCAGCA GCGACTATGT CACGTTCGAG ACAGGCAGAG CCTATAAGGA GCAGGAAGAC
    ATCGCTACTC AGGGCTGGTT CATTGGGTTG ATGTGTGCCG TCGCTTTATT GGTTCTGATT
    CTGCTCATTG TTTGTTTCAT CAAGAGGAGT CGTGGAGGAA AATACCCAGT GAGAGAGAAG
    AAAGACCTCC CCTTAGATCC CGTCGATCAT AAAGACCAAG ATGGATCATT TGACTATCAC
    AGCGACGAAG ACAACAAGCC GTTACAGGGC AGCCAAACGT CCCTGGACGG GAACGTTAAA
    GAAAGCGACG ACAGTCTCGT GGACTACGGC GAAGGAGGAG ACGGACAATT CAACGAGGAT
    GGTTCCTTCA TCGGCCAATA CACAGTAAAG AAGGACAAAG AGGAGACAGA AGGAAATGAG
    AGCTCAGAAG CCACATCACC TGTCAATGCC ATCTACTCTT TGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213675.1
    CDS298..4365
    Translation

    Target ORF information:

    RefSeq Version XM_017358186.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAATGCGTAA TATACCGGAA
    ACCCAGCCCA CCTTTCTATC TCCAACGGGC TTGTCCAGCA CCAAGATGGT GCTCAGAGGA
    GAAGAACTTC TCCTGGAGTG TATAGCAGCT GGAGTACCAA CTCCTACCAT TACCTGGTCC
    AAAAGAGGAG GAGACCTGCC AACGAAAAAA GTCAGGATTG GGAACTTCGA TAAAACCTTA
    CGCATTCTCA ATGTGTCTGA GGAAGACATT GGCAGCTACA CCTGCATGGC CAGCAACAGA
    ATTGGCAGCA TTCGCCACAC AGTTGAAGTC CAGGTCAAAG CTGCTCCATT TTGGCTCGAA
    AAGCCCATCA ATCTGGTTCT TGCCCCTGAT GAGAGTGGAC GGTTAGTGTG CCGTGCTAAC
    GGGAGTCCCA AACCCACTAT TCAATGGCTG GTGAATGGAG AACCCATTGA AAGTTCACTC
    CCCAACCCCA ATCGGAGAGT TCTTGATGAC ACGATCATAT TTGAGACGGT TCAGTTTGAA
    AGCAGTGCAG TTTACCAGTG TAACGCCTCC AATGAGCACG GTTATCTTCT GGCAAATGCT
    TTCGTCAGTG TTTTGGATAT GGCACCGCGG ATGCTTGGCC CGAAAAACCA GCTGATCAAA
    GTCATTGAAA ACAACAGGAC CTTCCTCGAG TGTCCTTTCT TTGGCTCACC GCTCCCTTTA
    CTTCGCTGGT TTAAGAACGG ACTTGGCAGT GGTTTGGATG GTGGTCACTA CAGGCTATAC
    CACAATGGCA CTTTGGAGAT CAAGCAGGCC CGGACAGAAG ACCAGGGCAC CTACACCTGC
    GTCGCCAGCA ACATTCTGGG CAAGATGGAG AATCAAGTGC GCCTAGAGGT TAAAGAGCCC
    ACAAGAATAG TCCGCGCACC TGAACATAAG ACGGAACCCA GAGGAACCAC AGTTCGATTC
    GACTGTCGAG TTAAACATGA TCCTTCCCTT CCTGCTACAG TCACATGGCT GAAAGATGAA
    AAGCCTCTGA GCTTTAGCTG GATTGGCCGG TTGAAAAAGG ATGATGAGTC CTTGACTATT
    TACAATGTCA ATCCAGACGA TGGAGGAACC TACACATGCA CAGCCAAAAC TGAGATAGAC
    GAGGACACTG CCTCAGCTCG CCTTACTGTT ACAGGTCGAC CCGACCCACC TCAGGATCTG
    GAACTCTCTG ACCCTTCAGC ACGCAGTGTC AGACTCACCT GGGTGCCCGG AAATGAAAAC
    AACAGTCCTG TTACACATTT CCTAGTCCAG TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG
    TGGCAGAACC TGTCAATTTA TGATTGGAAC CTAAACTCAG TTAACCTTCA GCTATCACCA
    TTCGTCAACT ACCAGTTCAG AGTGATTGCG ATCAATGCTG TGGGTCAGAG TCAACCCAGC
    CACCCCTCTG CACGTTATCA GACCAGCGGA GCCTCTCCTG ATGTTGTACC AAGTGGACTT
    AAAGGGTGGG GAAGCAAAAA AAACAACATG GAGATCACAT GGCAGGCCAT GCAGAACACA
    GAGAGGAATG GGCCAAACCT GCGATATGTG CTCTCCTGGA GAAGAAAAGA CACAGAAGAA
    GAGTGGAATA ACATCACCAC AGCCAAAACA AAACACGTTG TCCATAATAC AGACACCTAC
    GTTCCTTATG AGATCAAAAT ACAGGCTGTC AATGACTTTG GACATGGCCC AGAGTCAAAC
    ATGGTCATCG GTTATTCTGG GGAAGACAAA CCATCCGCTG CACCAACAGA TCTTAGGGTG
    TCAAAGATCA ACAGTACCAA AGTCAATATT CACTGGGAGC CTGTGGAGCA GAGCAGTGTA
    AATGGAGAAT TTAAAGAATA CAAGTTGTAC TACTGGCGGG AGGCCAGCCT CATAAAGGGC
    CTGAAAGTAA ATAAGACAAA GTTGCATAAG GGATTCTTTA CCACAGCAGA ACATCCCTCT
    GGCATCCTGA CAGAGCTCTA TCCATACAGC AAATATAAGA TGTATATGGT GGTTGCTAAC
    CGAGAACATG AAGGGCCGCA AAGCAATACT GTGGAGTTTT ACACCAAGGA GGGAGAGCCA
    GGGGCTCCAA AATACTTTAG GATTCTAAGA CACACGGACG TGGTTCACCT GAAGTGGGAC
    AAACCACTGG AGCCAAACGG TGTCCTGATT GGATACACAC TCCAGTACCA CACAGTGAAC
    GGGACACAGG CGGGAGAGAG GAAAGTCCAG AGTTTCCCAC CCAATGTGAC GGAGTACGTC
    ATGAATCTGC CTCACCGCTT CACGCGCTTC AAGTTCTTCC TAGCAGCCCG TACGCAGGTC
    GGAGCAGGAG AAGTGTACGC TGTCGAGTCT CCACACTTCA CCAATGAAGG CACCCCAGAT
    ACTGACTCTA CAGTTGTAGC CGTTAACTTT ACAGATGGCT TGATGTTCGA AATTCCTCCT
    CCTCCCCTTC CCCCCTCCAC TGACCCAAAC CCAACAATAT TCACCCCAGA ACCAACTCCA
    CCAGTCCCTA CAACTGCTGC TCCAACCAAA CCTACTACCA CCACCACCAC CACCACTACT
    ACTACTACAA CTTCTACTAC TACTACAACT ACCACAACTA CTACAACAAC CACCACTACT
    ACAGCAGCTT CCACCACAGT TGCTCCAGTT CGGCCCGTGC CTCCCAAAAC AGTGGATTGG
    AAAATGCTGG CCCCGAATGT AAAGATCTGG AATCTGACGG TGGATGCTAA CAATGACAAT
    GCTAACGTCA GCTGGAAGCA CAACTTCTCC GTTGACAGCA GCAAGTTTGT TGTAGAGTTC
    ACACTGGACA GTAATGGGTC GATGACGAGC ATTCAAGTAA ACCAGCAGCC TCACATTAAG
    CTGGCTGGGC TGGTGGCTGG GGCCAAGTAT CGGTTGCGGG TCTATTCGCA TGAGCACAAC
    TCCATCAGCA GCGACTATGT CACGTTCGAG ACAGGCAGAG CCTATAAGGA GCAGGAAGAC
    ATCGCTACTC AGGGCTGGTT CATTGGGTTG ATGTGTGCCG TCGCTTTATT GGTTCTGATT
    CTGCTCATTG TTTGTTTCAT CAAGAGGAGT CGTGGAGGAA AATACCCAGT GAGAGAGAAG
    AAAGACCTCC CCTTAGATCC CGTCGATCAT AAAGACCAAG ATGGATCATT TGACTATCAC
    AGCGACGAAG ACAACAAGCC GTTACAGGGC AGCCAAACGT CCCTGGACGG GAACGTTAAA
    GAAAGCGACG ACAGTCTCGT GGACTACGGC GAAGGAGGAG ACGGACAATT CAACGAGGAT
    GGTTCCTTCA TCGGCCAATA CACAGTAAAG AAGGACAAAG AGGAGACAGA AGGAAATGAG
    AGCTCAGAAG CCACATCACC TGTCAATGCC ATCTACTCTT TGGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54242
    Clone ID Related Accession (Same CDS sequence) XM_017358187.1
    Accession Version XM_017358187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofascin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGAGAATGAC AGCTCGTCTG CTTCAAATCG TAGTGCCTCT
    GCAGGTCGAC CCGACCCACC TCAGGATCTG GAACTCTCTG ACCCTTCAGC ACGCAGTGTC
    AGACTCACCT GGGTGCCCGG AAATGAAAAC AACAGTCCTG TTACACATTT CCTAGTCCAG
    TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG TGGCAGAACC TGTCAATTTA TGATTGGAAC
    CTAAACTCAG TTAACCTTCA GCTATCACCA TTCGTCAACT ACCAGTTCAG AGTGATTGCG
    ATCAATGCTG TGGGTCAGAG TCAACCCAGC CACCCCTCTG CACGTTATCA GACCAGCGGA
    GCCTCTCCTG ATGTTGTACC AAGTGGACTT AAAGGGTGGG GAAGCAAAAA AAACAACATG
    GAGATCACAT GGCAGGCCAT GCAGAACACA GAGAGGAATG GGCCAAACCT GCGATATGTG
    CTCTCCTGGA GAAGAAAAGA CACAGAAGAA GAGTGGAATA ACATCACCAC AGCCAAAACA
    AAACACGTTG TCCATAATAC AGACACCTAC GTTCCTTATG AGATCAAAAT ACAGGCTGTC
    AATGACTTTG GACATGGCCC AGAGTCAAAC ATGGTCATCG GTTATTCTGG GGAAGACAAA
    CCATCCGCTG CACCAACAGA TCTTAGGGTG TCAAAGATCA ACAGTACCAA AGTCAATATT
    CACTGGGAGC CTGTGGAGCA GAGCAGTGTA AATGGAGAAT TTAAAGAATA CAAGTTGTAC
    TACTGGCGGG AGGCCAGCCT CATAAAGGGC CTGAAAGTAA ATAAGACAAA GTTGCATAAG
    GGATTCTTTA CCACAGCAGA ACATCCCTCT GGCATCCTGA CAGAGCTCTA TCCATACAGC
    AAATATAAGA TGTATATGGT GGTTGCTAAC CGAGAACATG AAGGGCCGCA AAGCAATACT
    GTGGAGTTTT ACACCAAGGA GGGAGAGCCA GGGGCTCCAA AATACTTTAG GATTCTAAGA
    CACACGGACG TGGTTCACCT GAAGTGGGAC AAACCACTGG AGCCAAACGG TGTCCTGATT
    GGATACACAC TCCAGTACCA CACAGTGAAC GGGACACAGG CGGGAGAGAG GAAAGTCCAG
    AGTTTCCCAC CCAATGTGAC GGAGTACGTC ATGAATCTGC CTCACCGCTT CACGCGCTTC
    AAGTTCTTCC TAGCAGCCCG TACGCAGGTC GGAGCAGGAG AAGTGTACGC TGTCGAGTCT
    CCACACTTCA CCAATGAAGG CACCCCAGAT ACTGACTCTA CAGTTGTAGC CGTTAACTTT
    ACAGATGGCT TGATGTTCGA AATTCCTCCT CCTCCCCTTC CCCCCTCCAC TGACCCAAAC
    CCAACAATAT TCACCCCAGA ACCAACTCCA CCAGTCCCTA CAACTGCTGC TCCAACCAAA
    CCTACTACCA CCACCACCAC CACCACTACT ACTACTACAA CTTCTACTAC TACTACAACT
    ACCACAACTA CTACAACAAC CACCACTACT ACAGCAGCTT CCACCACAGT TGCTCCAGTT
    CGGCCCGTGC CTCCCAAAAC AGTGGATTGG AAAATGCTGG CCCCGAATGT AAAGATCTGG
    AATCTGACGG TGGATGCTAA CAATGACAAT GCTAACGTCA GCTGGAAGCA CAACTTCTCC
    GTTGACAGCA GCAAGTTTGT TGTAGAGTTC ACACTGGACA GTAATGGGTC GATGACGAGC
    ATTCAAGTAA ACCAGCAGCC TCACATTAAG CTGGCTGGGC TGGTGGCTGG GGCCAAGTAT
    CGGTTGCGGG TCTATTCGCA TGAGCACAAC TCCATCAGCA GCGACTATGT CACGTTCGAG
    ACAGGCAGAG CCTATAAGGA GCAGGAAGAC ATCGCTACTC AGGGCTGGTT CATTGGGTTG
    ATGTGTGCCG TCGCTTTATT GGTTCTGATT CTGCTCATTG TTTGTTTCAT CAAGAGGAGT
    CGTGGAGGAA AATACCCAGT GAGAGAGAAG AAAGACCTCC CCTTAGATCC CGTCGATCAT
    AAAGACCAAG ATGGATCATT TGACTATCAG TTCCGAAAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213676.1
    CDS298..4239
    Translation

    Target ORF information:

    RefSeq Version XM_017358187.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofascin homolog (chicken) a (nfasca), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTGGACAC AGAGGCGGTG GGCGGCACTC GCAGTGTTGT CAATAATCTC ACTGCTGTCA 
    GAGGAGGCGG CTGCTATTGA AGTCCCTCTG GACCCAAAGA TCCAGCAAGA GCTGAAACAG
    CCCCCCACCA TTGTGAAGCA GTCACTAAAG GACTACATTG TGGACCCCAG AGATAACATC
    ATCATTGAAT GCGAGGCCAA GGGGAACCCA GTGCCAACGT TTCAGTGGAG GCGGAATGGA
    AAGTTCTTTA ATGTGGGTAA GGACCCACGG GTGACCATGA GAAGTCGATC TGGAACACTA
    GAGATCAGAA GCAGTGGAAA ACCAGAAGAT TATGAAGGAG AATACCAGTG CTTCGCTTCC
    AACAACTTGG GCACAGCCAT TTCCAACAAA ATCCTGCTTC GAGTGTCAAA GTCTCCGCTG
    TGGCCAAAGG AGGTTTTGGA ACCGGTAACT GTGCGTGAGG GATCACCGCT CGTCCTGGCC
    TGCAACCCTC CTCCTGGTCT TCCCCCTCCT GAAACCTTCT GGATGGACAG CTTCATTATG
    CCTATAGCAC AGGACAGGAG GGTGTCAATG GGCCTGAACG GTGACTTGTT CTTCTCCAAC
    GTTCTAGCTA AAGATGCCAA GACTGACTAC AGCTGCAATG CTCGCTTCGA GTTCACACAC
    ACCATACAGC AGAAGAACCC CTACACACTT AAAGTGCAAA CAAAAGAACC TTATAATGAC
    ACCTTTCTCA ACTCAACTGA TGTGTATGGT GTGCGTAATA TACCGGAAAC CCAGCCCACC
    TTTCTATCTC CAACGGGCTT GTCCAGCACC AAGATGGTGC TCAGAGGAGA AGAACTTCTC
    CTGGAGTGTA TAGCAGCTGG AGTACCAACT CCTACCATTA CCTGGTCCAA AAGAGGAGGA
    GACCTGCCAA CGAAAAAAGT CAGGATTGGG AACTTCGATA AAACCTTACG CATTCTCAAT
    GTGTCTGAGG AAGACATTGG CAGCTACACC TGCATGGCCA GCAACAGAAT TGGCAGCATT
    CGCCACACAG TTGAAGTCCA GGTCAAAGCT GCTCCATTTT GGCTCGAAAA GCCCATCAAT
    CTGGTTCTTG CCCCTGATGA GAGTGGACGG TTAGTGTGCC GTGCTAACGG GAGTCCCAAA
    CCCACTATTC AATGGCTGGT GAATGGAGAA CCCATTGAAA GTTCACTCCC CAACCCCAAT
    CGGAGAGTTC TTGATGACAC GATCATATTT GAGACGGTTC AGTTTGAAAG CAGTGCAGTT
    TACCAGTGTA ACGCCTCCAA TGAGCACGGT TATCTTCTGG CAAATGCTTT CGTCAGTGTT
    TTGGATATGG CACCGCGGAT GCTTGGCCCG AAAAACCAGC TGATCAAAGT CATTGAAAAC
    AACAGGACCT TCCTCGAGTG TCCTTTCTTT GGCTCACCGC TCCCTTTACT TCGCTGGTTT
    AAGAACGGAC TTGGCAGTGG TTTGGATGGT GGTCACTACA GGCTATACCA CAATGGCACT
    TTGGAGATCA AGCAGGCCCG GACAGAAGAC CAGGGCACCT ACACCTGCGT CGCCAGCAAC
    ATTCTGGGCA AGATGGAGAA TCAAGTGCGC CTAGAGGTTA AAGAGCCCAC AAGAATAGTC
    CGCGCACCTG AACATAAGAC GGAACCCAGA GGAACCACAG TTCGATTCGA CTGTCGAGTT
    AAACATGATC CTTCCCTTCC TGCTACAGTC ACATGGCTGA AAGATGAAAA GCCTCTGAGC
    TTTAGCTGGA TTGGCCGGTT GAAAAAGGAT GATGAGTCCT TGACTATTTA CAATGTCAAT
    CCAGACGATG GAGGAACCTA CACATGCACA GCCAAAACTG AGATAGACGA GGACACTGCC
    TCAGCTCGCC TTACTGTTAC AGAGAATGAC AGCTCGTCTG CTTCAAATCG TAGTGCCTCT
    GCAGGTCGAC CCGACCCACC TCAGGATCTG GAACTCTCTG ACCCTTCAGC ACGCAGTGTC
    AGACTCACCT GGGTGCCCGG AAATGAAAAC AACAGTCCTG TTACACATTT CCTAGTCCAG
    TTTGAAGAGG ACAGGTGGGA GCCAGGGAAG TGGCAGAACC TGTCAATTTA TGATTGGAAC
    CTAAACTCAG TTAACCTTCA GCTATCACCA TTCGTCAACT ACCAGTTCAG AGTGATTGCG
    ATCAATGCTG TGGGTCAGAG TCAACCCAGC CACCCCTCTG CACGTTATCA GACCAGCGGA
    GCCTCTCCTG ATGTTGTACC AAGTGGACTT AAAGGGTGGG GAAGCAAAAA AAACAACATG
    GAGATCACAT GGCAGGCCAT GCAGAACACA GAGAGGAATG GGCCAAACCT GCGATATGTG
    CTCTCCTGGA GAAGAAAAGA CACAGAAGAA GAGTGGAATA ACATCACCAC AGCCAAAACA
    AAACACGTTG TCCATAATAC AGACACCTAC GTTCCTTATG AGATCAAAAT ACAGGCTGTC
    AATGACTTTG GACATGGCCC AGAGTCAAAC ATGGTCATCG GTTATTCTGG GGAAGACAAA
    CCATCCGCTG CACCAACAGA TCTTAGGGTG TCAAAGATCA ACAGTACCAA AGTCAATATT
    CACTGGGAGC CTGTGGAGCA GAGCAGTGTA AATGGAGAAT TTAAAGAATA CAAGTTGTAC
    TACTGGCGGG AGGCCAGCCT CATAAAGGGC CTGAAAGTAA ATAAGACAAA GTTGCATAAG
    GGATTCTTTA CCACAGCAGA ACATCCCTCT GGCATCCTGA CAGAGCTCTA TCCATACAGC
    AAATATAAGA TGTATATGGT GGTTGCTAAC CGAGAACATG AAGGGCCGCA AAGCAATACT
    GTGGAGTTTT ACACCAAGGA GGGAGAGCCA GGGGCTCCAA AATACTTTAG GATTCTAAGA
    CACACGGACG TGGTTCACCT GAAGTGGGAC AAACCACTGG AGCCAAACGG TGTCCTGATT
    GGATACACAC TCCAGTACCA CACAGTGAAC GGGACACAGG CGGGAGAGAG GAAAGTCCAG
    AGTTTCCCAC CCAATGTGAC GGAGTACGTC ATGAATCTGC CTCACCGCTT CACGCGCTTC
    AAGTTCTTCC TAGCAGCCCG TACGCAGGTC GGAGCAGGAG AAGTGTACGC TGTCGAGTCT
    CCACACTTCA CCAATGAAGG CACCCCAGAT ACTGACTCTA CAGTTGTAGC CGTTAACTTT
    ACAGATGGCT TGATGTTCGA AATTCCTCCT CCTCCCCTTC CCCCCTCCAC TGACCCAAAC
    CCAACAATAT TCACCCCAGA ACCAACTCCA CCAGTCCCTA CAACTGCTGC TCCAACCAAA
    CCTACTACCA CCACCACCAC CACCACTACT ACTACTACAA CTTCTACTAC TACTACAACT
    ACCACAACTA CTACAACAAC CACCACTACT ACAGCAGCTT CCACCACAGT TGCTCCAGTT
    CGGCCCGTGC CTCCCAAAAC AGTGGATTGG AAAATGCTGG CCCCGAATGT AAAGATCTGG
    AATCTGACGG TGGATGCTAA CAATGACAAT GCTAACGTCA GCTGGAAGCA CAACTTCTCC
    GTTGACAGCA GCAAGTTTGT TGTAGAGTTC ACACTGGACA GTAATGGGTC GATGACGAGC
    ATTCAAGTAA ACCAGCAGCC TCACATTAAG CTGGCTGGGC TGGTGGCTGG GGCCAAGTAT
    CGGTTGCGGG TCTATTCGCA TGAGCACAAC TCCATCAGCA GCGACTATGT CACGTTCGAG
    ACAGGCAGAG CCTATAAGGA GCAGGAAGAC ATCGCTACTC AGGGCTGGTT CATTGGGTTG
    ATGTGTGCCG TCGCTTTATT GGTTCTGATT CTGCTCATTG TTTGTTTCAT CAAGAGGAGT
    CGTGGAGGAA AATACCCAGT GAGAGAGAAG AAAGACCTCC CCTTAGATCC CGTCGATCAT
    AAAGACCAAG ATGGATCATT TGACTATCAG TTCCGAAAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.