MTO1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTO1
Entrez Gene ID	100009640
Full Name	mitochondrial tRNA translation optimization 1
Synonyms	zgc:158782
General protein information	Preferred Names mitochondrial tRNA translation optimization 1 Names protein MTO1 homolog, mitochondrial mitochondrial translation optimization 1 homolog
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306471.2	XP_009304746.1	protein MTO1 homolog, mitochondrial isoform X1
XM_009306471.3	XP_009304746.1	protein MTO1 homolog, mitochondrial isoform X1
XM_009306472.3	XP_009304747.1	protein MTO1 homolog, mitochondrial isoform X2
XM_021480003.1	XP_021335678.1	protein MTO1 homolog, mitochondrial isoform X3
XM_021480004.1	XP_021335679.1	protein MTO1 homolog, mitochondrial isoform X3
NM_001083009.1	NP_001076478.1	protein MTO1 homolog, mitochondrial
XM_009306472.2	XP_009304747.1	protein MTO1 homolog, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MTO1	XP_532202.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007661	XP_308209.4
Magnaporthe oryzae (rice blast fungus)	MGG_14754	XP_003713873.1
Danio rerio (zebrafish)	mto1	NP_001076478.1
Caenorhabditis elegans (roundworm)	F52H3.2	NP_496169.1
Eremothecium gossypii	AGOS_AFR255W	NP_985802.2
Oryza sativa (rice)	Os01g0960300	NP_001045465.1
Homo sapiens (human)	MTO1	NP_001116698.1
Pan troglodytes (chimpanzee)	MTO1	XP_003311451.1
Saccharomyces cerevisiae (baker's yeast)	MTO1	NP_011278.2
Kluyveromyces lactis	KLLA0A00935g	XP_451040.1
Schizosaccharomyces pombe (fission yeast)	SPBC30B4.06c	NP_595531.1
Neurospora crassa	NCU11170	XP_956189.2
Bos taurus (cattle)	MTO1	NP_001069537.1
Mus musculus (house mouse)	Mto1	NP_080934.2
Rattus norvegicus (Norway rat)	Mto1	NP_001100311.1
Gallus gallus (chicken)	MTO1	NP_001073232.1
Drosophila melanogaster (fruit fly)	CG4610	NP_611677.1
Arabidopsis thaliana (thale cress)	AT2G13440	NP_178974.1

Gene Ontology
Bibliography

Related articles in PubMed

Multiplicity of Buc copies in Atlantic salmon contrasts with loss of the germ cell determinant in primates, rodents and axolotl.
?kugor A, Tveiten H, Johnsen H, Andersen ?
BMC evolutionary biology16(1)232(2016 Oct)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03654	NM_001083009.1 Latest version!	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa17614	XM_009306472.3 Latest version!	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa48334	XM_009306471.3 Latest version!	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa148125	XM_021480003.1 Latest version!	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa148125	XM_021480004.1 Latest version!	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
ODa48334	XM_009306471.2	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17614	XM_009306472.2	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTO1 ( NM_001083009.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001076478.1 Danio rerio mitochondrial tRNA translation optimization 1 (mto1), mRNA.

Price & Availability↑

CloneID	ODa03654
Clone ID Related Accession (Same CDS sequence)	NM_001083009.1
Accession Version	NM_001083009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Danio rerio(zebrafish)
Product	protein MTO1 homolog, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133157.1. ##Evidence-Data-START## Transcript exon combination :: BC133157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTGGTAA GAAGATTGCA GTTTCTAAAG ACTCTCTCCA CCTGCTCTAT TGAAGCTTCT 
AATATACACA CTCAACATTA TGATGTAATT GTGGTTGGCG GTGGTCACGC GGGCTCTGAA 
GCAGTGGCTG CTGCCAGCAG AATTGGAGCA AAGACACTTC TCATCACACA GAAAATCCAG 
ACTATTGGGG CACTCTCGTG TAACCCATCA CTCGGGGGTG TCGGGAAGGG TCATTTAGTA 
AGGGAGATTG ACGCTCTTGA TGGTGTGATG GGACGGGCTG GAGATTGGGC TGGAATTCAT 
TTTTCCATTC TCAACCGCAG CAAGGGACCA GCAGTCTGGG GCCTCAGAGC CCAACTGGAC 
CGTCAGCGAT ATAAAGACTT TATGCAGTCA GAGCTTTTAT CTACACCCTT AGTGACCATC 
CTAGAGGGTT CTGTTCAGGA CCTTCTGTTG ACTGAGGCTG ATCCAGGGAA ACCAGGAAAA 
CATAAAGTTT ATGGAATATG CCTGGCTAAT GGAGGTGGTG AAATCCATTC CAGCTCTGTG 
GTTCTGACCA CTGGCACCTT CCTGTCAGGT GCTCTCTTCA TGGGCCAGAA CACTACTCCA 
GGGGGCCGAA TGGGGGATCC ACCTTCCTGT GCGGGTCTTT CTCATAACTT AAAAAACGTT 
TTGGGGTTGA AGATTGGTAG GCTAAGGACT GGTACTCCTC CCCGTATTGT CAAGGATTCA 
ATTGACTTCT CCCTCACCTC TGTTTGTTTG CCTGACTCAA GTCCTACTCC ATTCAGCTTC 
ATTAACAAAC ACACACACTG CACGCCTAAA GAGCAACTTC CATGTTTTTT GACATACACC 
ACTCCTGGTG TAGAAAAAGT GGTGAAAGAG AGCAGCCATC TCAACTCACA CATACAGCAA 
GACATTAAAG GACCCAGGTA CTGCCCCTCC ATTGAGTCAA AGGTGCTGCG GTTTCCAGGG 
CGACAGCATC AAGTATGGCT GGAACCAGAG GGTTTGACCT CCTCTCTCAT GTACCCTCAG 
GGTTTGTCTA TGACTATGCC ACTGGAGATG CAGCTCCGAC TCATCAGGGA GATCCCTGGG 
CTGCAGCATG CCAAAATTCA AACTCCAGGT TATGGAGTAC AATATGACTT TGTCTGTCCA 
ACACAACTTT TCCCATGGCT ACAGTTAAAG AACACTCAGG GTCTATTCTT GGCTGGGCAA 
ATAAATGGGA CCACTGGCTA TGAGGAGGCA GCTGCCCAGG GCTTGTGGGC TGGGGTCAAT 
GCAGGTCGAA CATCTCTCTC CATGTCTCCA TTGTCCTTGT CACGCACACA AAGCTACATC 
GGAGTTTTAA TTGATGATCT GGTTTGTCAT GGAGTAACAG AGCCATATCG TATGTTCACA 
AGCAGAGCTG AGTTTAGAAC CTCCCTCAGA CCAGACAACG CTGACCTGCG ACTAACTATA 
AGAGGTTTTG AGGAGATTGG TTGTGTGTCT GAACAGCGCT ATAATAAAGC TCTTCTGGTT 
CAGGCAAGTT TGAAGCAGGC TCTATCTACT CTGCAGTCTA TCCATCTCTC CGCTGCTCGC 
TGGAGGGATA AACTGGGAGG TGTGACCATT AGCAAGACAA AGAGTTCCAT TCTGAATGGA 
GCGGAGGTCT TGCAGTATAA AGATGTTACT TTTCAGATGT TGGCATCTTC CTTCCAAGAA 
CTATTTGGCC CCCATCTAGA GATTTCAGAG AGATTGAAAG TTGAAGCTCT ATACCATCCA 
CACTGTGAGA AGCAGAGAAG AGAGATGGAG AAAATGCAAA CAGAGGAAAG TTTGTCATTG 
CCAAATGACA TTGACTATCT GGCTCTACCC ATCTCGCTTT CTCAGGAGGT CCGAGAGATT 
TTGGACAGAG TGCGACCAAG CACACTGGGA GCTGCAGCCC GTCTGCCAGG AATTACTCCA 
GCAGCTATAG TTCATCTGTT CAACTACGTG CACAGAAACA AACACAGAAA TCATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076478.1
CDS	161..2140
Translation	MLVRRLQFLKTLSTCSIEASNIHTQHYDVIVVGGGHAGSEAVAAASRIGAKTLLITQKIQTIGALSCNPSLGGVGKGHLVREIDALDGVMGRAGDWAGIHFSILNRSKGPAVWGLRAQLDRQRYKDFMQSELLSTPLVTILEGSVQDLLLTEADPGKPGKHKVYGICLANGGGEIHSSSVVLTTGTFLSGALFMGQNTTPGGRMGDPPSCAGLSHNLKNVLGLKIGRLRTGTPPRIVKDSIDFSLTSVCLPDSSPTPFSFINKHTHCTPKEQLPCFLTYTTPGVEKVVKESSHLNSHIQQDIKGPRYCPSIESKVLRFPGRQHQVWLEPEGLTSSLMYPQGLSMTMPLEMQLRLIREIPGLQHAKIQTPGYGVQYDFVCPTQLFPWLQLKNTQGLFLAGQINGTTGYEEAAAQGLWAGVNAGRTSLSMSPLSLSRTQSYIGVLIDDLVCHGVTEPYRMFTSRAEFRTSLRPDNADLRLTIRGFEEIGCVSEQRYNKALLVQASLKQALSTLQSIHLSAARWRDKLGGVTISKTKSSILNGAEVLQYKDVTFQMLASSFQELFGPHLEISERLKVEALYHPHCEKQRREMEKMQTEESLSLPNDIDYLALPISLSQEVREILDRVRPSTLGAAARLPGITPAAIVHLFNYVHRNKHRNHM

Target ORF information:

RefSeq Version	NM_001083009.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083009.1

ORF Insert Sequence:

ATGTTGGTAA GAAGATTGCA GTTTCTAAAG ACTCTCTCCA CCTGCTCTAT TGAAGCTTCT 
AATATACACA CTCAACATTA TGATGTAATT GTGGTTGGCG GTGGTCACGC GGGCTCTGAA 
GCAGTGGCTG CTGCCAGCAG AATTGGAGCA AAGACACTTC TCATCACACA GAAAATCCAG 
ACTATTGGGG CACTCTCGTG TAACCCATCA CTCGGGGGTG TCGGGAAGGG TCATTTAGTA 
AGGGAGATTG ACGCTCTTGA TGGTGTGATG GGACGGGCTG GAGATTGGGC TGGAATTCAT 
TTTTCCATTC TCAACCGCAG CAAGGGACCA GCAGTCTGGG GCCTCAGAGC CCAACTGGAC 
CGTCAGCGAT ATAAAGACTT TATGCAGTCA GAGCTTTTAT CTACACCCTT AGTGACCATC 
CTAGAGGGTT CTGTTCAGGA CCTTCTGTTG ACTGAGGCTG ATCCAGGGAA ACCAGGAAAA 
CATAAAGTTT ATGGAATATG CCTGGCTAAT GGAGGTGGTG AAATCCATTC CAGCTCTGTG 
GTTCTGACCA CTGGCACCTT CCTGTCAGGT GCTCTCTTCA TGGGCCAGAA CACTACTCCA 
GGGGGCCGAA TGGGGGATCC ACCTTCCTGT GCGGGTCTTT CTCATAACTT AAAAAACGTT 
TTGGGGTTGA AGATTGGTAG GCTAAGGACT GGTACTCCTC CCCGTATTGT CAAGGATTCA 
ATTGACTTCT CCCTCACCTC TGTTTGTTTG CCTGACTCAA GTCCTACTCC ATTCAGCTTC 
ATTAACAAAC ACACACACTG CACGCCTAAA GAGCAACTTC CATGTTTTTT GACATACACC 
ACTCCTGGTG TAGAAAAAGT GGTGAAAGAG AGCAGCCATC TCAACTCACA CATACAGCAA 
GACATTAAAG GACCCAGGTA CTGCCCCTCC ATTGAGTCAA AGGTGCTGCG GTTTCCAGGG 
CGACAGCATC AAGTATGGCT GGAACCAGAG GGTTTGACCT CCTCTCTCAT GTACCCTCAG 
GGTTTGTCTA TGACTATGCC ACTGGAGATG CAGCTCCGAC TCATCAGGGA GATCCCTGGG 
CTGCAGCATG CCAAAATTCA AACTCCAGGT TATGGAGTAC AATATGACTT TGTCTGTCCA 
ACACAACTTT TCCCATGGCT ACAGTTAAAG AACACTCAGG GTCTATTCTT GGCTGGGCAA 
ATAAATGGGA CCACTGGCTA TGAGGAGGCA GCTGCCCAGG GCTTGTGGGC TGGGGTCAAT 
GCAGGTCGAA CATCTCTCTC CATGTCTCCA TTGTCCTTGT CACGCACACA AAGCTACATC 
GGAGTTTTAA TTGATGATCT GGTTTGTCAT GGAGTAACAG AGCCATATCG TATGTTCACA 
AGCAGAGCTG AGTTTAGAAC CTCCCTCAGA CCAGACAACG CTGACCTGCG ACTAACTATA 
AGAGGTTTTG AGGAGATTGG TTGTGTGTCT GAACAGCGCT ATAATAAAGC TCTTCTGGTT 
CAGGCAAGTT TGAAGCAGGC TCTATCTACT CTGCAGTCTA TCCATCTCTC CGCTGCTCGC 
TGGAGGGATA AACTGGGAGG TGTGACCATT AGCAAGACAA AGAGTTCCAT TCTGAATGGA 
GCGGAGGTCT TGCAGTATAA AGATGTTACT TTTCAGATGT TGGCATCTTC CTTCCAAGAA 
CTATTTGGCC CCCATCTAGA GATTTCAGAG AGATTGAAAG TTGAAGCTCT ATACCATCCA 
CACTGTGAGA AGCAGAGAAG AGAGATGGAG AAAATGCAAA CAGAGGAAAG TTTGTCATTG 
CCAAATGACA TTGACTATCT GGCTCTACCC ATCTCGCTTT CTCAGGAGGT CCGAGAGATT 
TTGGACAGAG TGCGACCAAG CACACTGGGA GCTGCAGCCC GTCTGCCAGG AATTACTCCA 
GCAGCTATAG TTCATCTGTT CAACTACGTG CACAGAAACA AACACAGAAA TCATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTO1 ( XM_009306472.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304747.1 Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17614
Clone ID Related Accession (Same CDS sequence)	XM_009306472.3 , XM_009306472.2
Accession Version	XM_009306472.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein MTO1 homolog, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306472.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAA GAAGATTGCA GTTTCTAAAG ACTCTCTCCA CCTGCTCTAT TGAAGCTTCT 
AATATACACA CTCAACATTA TGATGTAATT GTGGTTGGCG GTGGTCACGC GGGCTCTGAA 
GCAGTGGCTG CTGCCAGCAG AATTGGAGCA AAGACACTTC TCATCACACA GAAAATCCAG 
ACTATTGGGG CACTCTCGTG TAACCCATCA CTCGGGGGTG TCGGGAAGGG TCATTTAGTA 
AGGGAGATTG ACGCTCTTGA TGGTGTGATG GGACGGGCTG GAGATTGGGC TGGAATTCAT 
TTTTCCATTC TCAACCGCAG CAAGGGACCA GCAGTCTGGG GCCTCAGAGC CCAACTGGAC 
CGTCAGCGAT ATAAAGACTT TATGCAGTCA GAGCTTTTAT CTACACCCTT AGTGACCATC 
CTAGAGGGTT CTGTTCAGGA CCTTCTGTTG ACTGAGGCTG ATCCAGGGAA ACCAGGAAAA 
CATAAAGTTT ATGGAATATG CCTGGCTAAT GGAGGTGGTG AAATCCATTC CAGCTCTGTG 
GTTCTGACCA CTGGCACCTT CCTGTCAGGT GCTCTCTTCA TGGGCCAGAA CACTACTCCA 
GGGGGCCGAA TGGGGGATCC ACCTTCCTGT GCGGGTCTTT CTCATAACTT AAAAAACGTT 
TTGGGGTTGA AGATTGGTAG GCTAAGGACT GGTACTCCTC CCCGTATTGT CAAGGATTCA 
ATTGACTTCT CCCTCACCTC TGTTTGTTTG CCTGACTCAA GTCCTACTCC ATTCAGCTTC 
ATTAACAAAC ACACACACTG CACGCCTAAA GAGCAACTTC CATGTTTTTT GACATACACC 
ACTCCTGGTG TAGAAAAAGT GGTGAAAGAG AGCAGCCATC TCAACTCACA CATACAGCAA 
GACATTAAAG GACCCAGGTA CTGCCCCTCC ATTGAGTCAA AGGTGCTGCG GTTTCCAGGG 
CGACAGCATC AAGTATGGCT GGAACCAGAG GGTTTGACCT CCTCTCTCAT GTACCCTCAG 
GGTTTGTCTA TGACTATGCC ACTGGAGATG CAGCTCCGAC TCATCAGGGA GATCCCTGGG 
CTGCAGCATG CCAAAATTCA AACTCCAGGT TATGGAGTAC AATATGACTT TGTCTGTCCA 
ACACAACTTT TCCCATGGCT ACAGTTAAAG AACACTCAGG GTCTATTCTT GGCTGGGCAA 
ATAAATGGGA CCACTGGCTA TGAGGAGGCA GCTGCCCAGG GCTTGTGGGC TGGGGTCAAT 
GCAGGTCGAA CATCTCTCTC CATGTCTCCA TTGTCCTTGT CACGCACACA AAGCTACATC 
GGAGTTTTAA TTGATGATCT GGTTTGTCAT GGAGTAACAG AGCCATATCG TATGTTCACA 
AGCAGAGCTG AGTTTAGAAC CTCCCTCAGA CCAGACAACG CTGACCTGCG ACTAACTATA 
AGAGGTTTTG AGGAGATTGG TTGTGTGTCT GAACAGCGCT ATAATAAAGC TCTTCTGGTT 
CAGGCAAGTT TGAAGCAGGC TCTATCTACT CTGCAGTCCA TCCATCTCTC CGCTGCTCGC 
TGGAGGGATA AACTGGGAGG TGTGACCATT AGCAAGACAA AGAGTTCCAT TCTGAATGGA 
GTGGAGGTCT TGCAGTATAA AGATGTTACT TTTCAGATGT TGGCATCTTC CTTCCAAGAA 
CTATTTGGCC CCCATCTAGA GATTTCAGAG AGATTGAAAG TTGAAGCTCT ATACCATCCA 
CACTGTGAGA AGCAGAGAAG AGAGATGGAG AAAATGCAAA CAGAGGAAAG TTTGTCATTG 
CCAAATGACA TTGACTATCT GGCTCTACCC ATCTCGCTTT CTCAGGAGGT CCGAGAGATT 
CTGGACAGAG TGCGACCAAG CACACTGGGA GCTGCAGCCC GTCTGCCAGG AATTACTCCA 
GCAGCTATAG TTCATCTGTT CAACTACGTG CACAGAAACA AACACAGAAA TCATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304747.1
CDS	70..2049
Translation	MLVRRLQFLKTLSTCSIEASNIHTQHYDVIVVGGGHAGSEAVAAASRIGAKTLLITQKIQTIGALSCNPSLGGVGKGHLVREIDALDGVMGRAGDWAGIHFSILNRSKGPAVWGLRAQLDRQRYKDFMQSELLSTPLVTILEGSVQDLLLTEADPGKPGKHKVYGICLANGGGEIHSSSVVLTTGTFLSGALFMGQNTTPGGRMGDPPSCAGLSHNLKNVLGLKIGRLRTGTPPRIVKDSIDFSLTSVCLPDSSPTPFSFINKHTHCTPKEQLPCFLTYTTPGVEKVVKESSHLNSHIQQDIKGPRYCPSIESKVLRFPGRQHQVWLEPEGLTSSLMYPQGLSMTMPLEMQLRLIREIPGLQHAKIQTPGYGVQYDFVCPTQLFPWLQLKNTQGLFLAGQINGTTGYEEAAAQGLWAGVNAGRTSLSMSPLSLSRTQSYIGVLIDDLVCHGVTEPYRMFTSRAEFRTSLRPDNADLRLTIRGFEEIGCVSEQRYNKALLVQASLKQALSTLQSIHLSAARWRDKLGGVTISKTKSSILNGVEVLQYKDVTFQMLASSFQELFGPHLEISERLKVEALYHPHCEKQRREMEKMQTEESLSLPNDIDYLALPISLSQEVREILDRVRPSTLGAAARLPGITPAAIVHLFNYVHRNKHRNHM

Target ORF information:

RefSeq Version	XM_009306472.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306472.3

ORF Insert Sequence:

ATGTTGGTAA GAAGATTGCA GTTTCTAAAG ACTCTCTCCA CCTGCTCTAT TGAAGCTTCT 
AATATACACA CTCAACATTA TGATGTAATT GTGGTTGGCG GTGGTCACGC GGGCTCTGAA 
GCAGTGGCTG CTGCCAGCAG AATTGGAGCA AAGACACTTC TCATCACACA GAAAATCCAG 
ACTATTGGGG CACTCTCGTG TAACCCATCA CTCGGGGGTG TCGGGAAGGG TCATTTAGTA 
AGGGAGATTG ACGCTCTTGA TGGTGTGATG GGACGGGCTG GAGATTGGGC TGGAATTCAT 
TTTTCCATTC TCAACCGCAG CAAGGGACCA GCAGTCTGGG GCCTCAGAGC CCAACTGGAC 
CGTCAGCGAT ATAAAGACTT TATGCAGTCA GAGCTTTTAT CTACACCCTT AGTGACCATC 
CTAGAGGGTT CTGTTCAGGA CCTTCTGTTG ACTGAGGCTG ATCCAGGGAA ACCAGGAAAA 
CATAAAGTTT ATGGAATATG CCTGGCTAAT GGAGGTGGTG AAATCCATTC CAGCTCTGTG 
GTTCTGACCA CTGGCACCTT CCTGTCAGGT GCTCTCTTCA TGGGCCAGAA CACTACTCCA 
GGGGGCCGAA TGGGGGATCC ACCTTCCTGT GCGGGTCTTT CTCATAACTT AAAAAACGTT 
TTGGGGTTGA AGATTGGTAG GCTAAGGACT GGTACTCCTC CCCGTATTGT CAAGGATTCA 
ATTGACTTCT CCCTCACCTC TGTTTGTTTG CCTGACTCAA GTCCTACTCC ATTCAGCTTC 
ATTAACAAAC ACACACACTG CACGCCTAAA GAGCAACTTC CATGTTTTTT GACATACACC 
ACTCCTGGTG TAGAAAAAGT GGTGAAAGAG AGCAGCCATC TCAACTCACA CATACAGCAA 
GACATTAAAG GACCCAGGTA CTGCCCCTCC ATTGAGTCAA AGGTGCTGCG GTTTCCAGGG 
CGACAGCATC AAGTATGGCT GGAACCAGAG GGTTTGACCT CCTCTCTCAT GTACCCTCAG 
GGTTTGTCTA TGACTATGCC ACTGGAGATG CAGCTCCGAC TCATCAGGGA GATCCCTGGG 
CTGCAGCATG CCAAAATTCA AACTCCAGGT TATGGAGTAC AATATGACTT TGTCTGTCCA 
ACACAACTTT TCCCATGGCT ACAGTTAAAG AACACTCAGG GTCTATTCTT GGCTGGGCAA 
ATAAATGGGA CCACTGGCTA TGAGGAGGCA GCTGCCCAGG GCTTGTGGGC TGGGGTCAAT 
GCAGGTCGAA CATCTCTCTC CATGTCTCCA TTGTCCTTGT CACGCACACA AAGCTACATC 
GGAGTTTTAA TTGATGATCT GGTTTGTCAT GGAGTAACAG AGCCATATCG TATGTTCACA 
AGCAGAGCTG AGTTTAGAAC CTCCCTCAGA CCAGACAACG CTGACCTGCG ACTAACTATA 
AGAGGTTTTG AGGAGATTGG TTGTGTGTCT GAACAGCGCT ATAATAAAGC TCTTCTGGTT 
CAGGCAAGTT TGAAGCAGGC TCTATCTACT CTGCAGTCCA TCCATCTCTC CGCTGCTCGC 
TGGAGGGATA AACTGGGAGG TGTGACCATT AGCAAGACAA AGAGTTCCAT TCTGAATGGA 
GTGGAGGTCT TGCAGTATAA AGATGTTACT TTTCAGATGT TGGCATCTTC CTTCCAAGAA 
CTATTTGGCC CCCATCTAGA GATTTCAGAG AGATTGAAAG TTGAAGCTCT ATACCATCCA 
CACTGTGAGA AGCAGAGAAG AGAGATGGAG AAAATGCAAA CAGAGGAAAG TTTGTCATTG 
CCAAATGACA TTGACTATCT GGCTCTACCC ATCTCGCTTT CTCAGGAGGT CCGAGAGATT 
CTGGACAGAG TGCGACCAAG CACACTGGGA GCTGCAGCCC GTCTGCCAGG AATTACTCCA 
GCAGCTATAG TTCATCTGTT CAACTACGTG CACAGAAACA AACACAGAAA TCATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTO1 ( XM_009306472.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304747.1 Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17614
Clone ID Related Accession (Same CDS sequence)	XM_009306472.3 , XM_009306472.2
Accession Version	XM_009306472.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein MTO1 homolog, mitochondrial isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAA GAAGATTGCA GTTTCTAAAG ACTCTCTCCA CCTGCTCTAT TGAAGCTTCT 
AATATACACA CTCAACATTA TGATGTAATT GTGGTTGGCG GTGGTCACGC GGGCTCTGAA 
GCAGTGGCTG CTGCCAGCAG AATTGGAGCA AAGACACTTC TCATCACACA GAAAATCCAG 
ACTATTGGGG CACTCTCGTG TAACCCATCA CTCGGGGGTG TCGGGAAGGG TCATTTAGTA 
AGGGAGATTG ACGCTCTTGA TGGTGTGATG GGACGGGCTG GAGATTGGGC TGGAATTCAT 
TTTTCCATTC TCAACCGCAG CAAGGGACCA GCAGTCTGGG GCCTCAGAGC CCAACTGGAC 
CGTCAGCGAT ATAAAGACTT TATGCAGTCA GAGCTTTTAT CTACACCCTT AGTGACCATC 
CTAGAGGGTT CTGTTCAGGA CCTTCTGTTG ACTGAGGCTG ATCCAGGGAA ACCAGGAAAA 
CATAAAGTTT ATGGAATATG CCTGGCTAAT GGAGGTGGTG AAATCCATTC CAGCTCTGTG 
GTTCTGACCA CTGGCACCTT CCTGTCAGGT GCTCTCTTCA TGGGCCAGAA CACTACTCCA 
GGGGGCCGAA TGGGGGATCC ACCTTCCTGT GCGGGTCTTT CTCATAACTT AAAAAACGTT 
TTGGGGTTGA AGATTGGTAG GCTAAGGACT GGTACTCCTC CCCGTATTGT CAAGGATTCA 
ATTGACTTCT CCCTCACCTC TGTTTGTTTG CCTGACTCAA GTCCTACTCC ATTCAGCTTC 
ATTAACAAAC ACACACACTG CACGCCTAAA GAGCAACTTC CATGTTTTTT GACATACACC 
ACTCCTGGTG TAGAAAAAGT GGTGAAAGAG AGCAGCCATC TCAACTCACA CATACAGCAA 
GACATTAAAG GACCCAGGTA CTGCCCCTCC ATTGAGTCAA AGGTGCTGCG GTTTCCAGGG 
CGACAGCATC AAGTATGGCT GGAACCAGAG GGTTTGACCT CCTCTCTCAT GTACCCTCAG 
GGTTTGTCTA TGACTATGCC ACTGGAGATG CAGCTCCGAC TCATCAGGGA GATCCCTGGG 
CTGCAGCATG CCAAAATTCA AACTCCAGGT TATGGAGTAC AATATGACTT TGTCTGTCCA 
ACACAACTTT TCCCATGGCT ACAGTTAAAG AACACTCAGG GTCTATTCTT GGCTGGGCAA 
ATAAATGGGA CCACTGGCTA TGAGGAGGCA GCTGCCCAGG GCTTGTGGGC TGGGGTCAAT 
GCAGGTCGAA CATCTCTCTC CATGTCTCCA TTGTCCTTGT CACGCACACA AAGCTACATC 
GGAGTTTTAA TTGATGATCT GGTTTGTCAT GGAGTAACAG AGCCATATCG TATGTTCACA 
AGCAGAGCTG AGTTTAGAAC CTCCCTCAGA CCAGACAACG CTGACCTGCG ACTAACTATA 
AGAGGTTTTG AGGAGATTGG TTGTGTGTCT GAACAGCGCT ATAATAAAGC TCTTCTGGTT 
CAGGCAAGTT TGAAGCAGGC TCTATCTACT CTGCAGTCCA TCCATCTCTC CGCTGCTCGC 
TGGAGGGATA AACTGGGAGG TGTGACCATT AGCAAGACAA AGAGTTCCAT TCTGAATGGA 
GTGGAGGTCT TGCAGTATAA AGATGTTACT TTTCAGATGT TGGCATCTTC CTTCCAAGAA 
CTATTTGGCC CCCATCTAGA GATTTCAGAG AGATTGAAAG TTGAAGCTCT ATACCATCCA 
CACTGTGAGA AGCAGAGAAG AGAGATGGAG AAAATGCAAA CAGAGGAAAG TTTGTCATTG 
CCAAATGACA TTGACTATCT GGCTCTACCC ATCTCGCTTT CTCAGGAGGT CCGAGAGATT 
CTGGACAGAG TGCGACCAAG CACACTGGGA GCTGCAGCCC GTCTGCCAGG AATTACTCCA 
GCAGCTATAG TTCATCTGTT CAACTACGTG CACAGAAACA AACACAGAAA TCATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304747.1
CDS	362..2341
Translation	MLVRRLQFLKTLSTCSIEASNIHTQHYDVIVVGGGHAGSEAVAAASRIGAKTLLITQKIQTIGALSCNPSLGGVGKGHLVREIDALDGVMGRAGDWAGIHFSILNRSKGPAVWGLRAQLDRQRYKDFMQSELLSTPLVTILEGSVQDLLLTEADPGKPGKHKVYGICLANGGGEIHSSSVVLTTGTFLSGALFMGQNTTPGGRMGDPPSCAGLSHNLKNVLGLKIGRLRTGTPPRIVKDSIDFSLTSVCLPDSSPTPFSFINKHTHCTPKEQLPCFLTYTTPGVEKVVKESSHLNSHIQQDIKGPRYCPSIESKVLRFPGRQHQVWLEPEGLTSSLMYPQGLSMTMPLEMQLRLIREIPGLQHAKIQTPGYGVQYDFVCPTQLFPWLQLKNTQGLFLAGQINGTTGYEEAAAQGLWAGVNAGRTSLSMSPLSLSRTQSYIGVLIDDLVCHGVTEPYRMFTSRAEFRTSLRPDNADLRLTIRGFEEIGCVSEQRYNKALLVQASLKQALSTLQSIHLSAARWRDKLGGVTISKTKSSILNGVEVLQYKDVTFQMLASSFQELFGPHLEISERLKVEALYHPHCEKQRREMEKMQTEESLSLPNDIDYLALPISLSQEVREILDRVRPSTLGAAARLPGITPAAIVHLFNYVHRNKHRNHM

Target ORF information:

RefSeq Version	XM_009306472.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306472.2

ORF Insert Sequence:

ATGTTGGTAA GAAGATTGCA GTTTCTAAAG ACTCTCTCCA CCTGCTCTAT TGAAGCTTCT 
AATATACACA CTCAACATTA TGATGTAATT GTGGTTGGCG GTGGTCACGC GGGCTCTGAA 
GCAGTGGCTG CTGCCAGCAG AATTGGAGCA AAGACACTTC TCATCACACA GAAAATCCAG 
ACTATTGGGG CACTCTCGTG TAACCCATCA CTCGGGGGTG TCGGGAAGGG TCATTTAGTA 
AGGGAGATTG ACGCTCTTGA TGGTGTGATG GGACGGGCTG GAGATTGGGC TGGAATTCAT 
TTTTCCATTC TCAACCGCAG CAAGGGACCA GCAGTCTGGG GCCTCAGAGC CCAACTGGAC 
CGTCAGCGAT ATAAAGACTT TATGCAGTCA GAGCTTTTAT CTACACCCTT AGTGACCATC 
CTAGAGGGTT CTGTTCAGGA CCTTCTGTTG ACTGAGGCTG ATCCAGGGAA ACCAGGAAAA 
CATAAAGTTT ATGGAATATG CCTGGCTAAT GGAGGTGGTG AAATCCATTC CAGCTCTGTG 
GTTCTGACCA CTGGCACCTT CCTGTCAGGT GCTCTCTTCA TGGGCCAGAA CACTACTCCA 
GGGGGCCGAA TGGGGGATCC ACCTTCCTGT GCGGGTCTTT CTCATAACTT AAAAAACGTT 
TTGGGGTTGA AGATTGGTAG GCTAAGGACT GGTACTCCTC CCCGTATTGT CAAGGATTCA 
ATTGACTTCT CCCTCACCTC TGTTTGTTTG CCTGACTCAA GTCCTACTCC ATTCAGCTTC 
ATTAACAAAC ACACACACTG CACGCCTAAA GAGCAACTTC CATGTTTTTT GACATACACC 
ACTCCTGGTG TAGAAAAAGT GGTGAAAGAG AGCAGCCATC TCAACTCACA CATACAGCAA 
GACATTAAAG GACCCAGGTA CTGCCCCTCC ATTGAGTCAA AGGTGCTGCG GTTTCCAGGG 
CGACAGCATC AAGTATGGCT GGAACCAGAG GGTTTGACCT CCTCTCTCAT GTACCCTCAG 
GGTTTGTCTA TGACTATGCC ACTGGAGATG CAGCTCCGAC TCATCAGGGA GATCCCTGGG 
CTGCAGCATG CCAAAATTCA AACTCCAGGT TATGGAGTAC AATATGACTT TGTCTGTCCA 
ACACAACTTT TCCCATGGCT ACAGTTAAAG AACACTCAGG GTCTATTCTT GGCTGGGCAA 
ATAAATGGGA CCACTGGCTA TGAGGAGGCA GCTGCCCAGG GCTTGTGGGC TGGGGTCAAT 
GCAGGTCGAA CATCTCTCTC CATGTCTCCA TTGTCCTTGT CACGCACACA AAGCTACATC 
GGAGTTTTAA TTGATGATCT GGTTTGTCAT GGAGTAACAG AGCCATATCG TATGTTCACA 
AGCAGAGCTG AGTTTAGAAC CTCCCTCAGA CCAGACAACG CTGACCTGCG ACTAACTATA 
AGAGGTTTTG AGGAGATTGG TTGTGTGTCT GAACAGCGCT ATAATAAAGC TCTTCTGGTT 
CAGGCAAGTT TGAAGCAGGC TCTATCTACT CTGCAGTCCA TCCATCTCTC CGCTGCTCGC 
TGGAGGGATA AACTGGGAGG TGTGACCATT AGCAAGACAA AGAGTTCCAT TCTGAATGGA 
GTGGAGGTCT TGCAGTATAA AGATGTTACT TTTCAGATGT TGGCATCTTC CTTCCAAGAA 
CTATTTGGCC CCCATCTAGA GATTTCAGAG AGATTGAAAG TTGAAGCTCT ATACCATCCA 
CACTGTGAGA AGCAGAGAAG AGAGATGGAG AAAATGCAAA CAGAGGAAAG TTTGTCATTG 
CCAAATGACA TTGACTATCT GGCTCTACCC ATCTCGCTTT CTCAGGAGGT CCGAGAGATT 
CTGGACAGAG TGCGACCAAG CACACTGGGA GCTGCAGCCC GTCTGCCAGG AATTACTCCA 
GCAGCTATAG TTCATCTGTT CAACTACGTG CACAGAAACA AACACAGAAA TCATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTO1 ( XM_009306471.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304746.1 Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48334
Clone ID Related Accession (Same CDS sequence)	XM_009306471.2 , XM_009306471.3
Accession Version	XM_009306471.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein MTO1 homolog, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCAG TCAAACCAGC GGAAACACAG TCAAGCCAGC ACGATATATA TATTGAGATT 
TCAAAAAATT CTGTTACCCT CCAAGTTAGT GTACCACAAT TGTCACACAT GTTGGTAAGA 
AGATTGCAGT TTCTAAAGAC TCTCTCCACC TGCTCTATTG AAGCTTCTAA TATACACACT 
CAACATTATG ATGTAATTGT GGTTGGCGGT GGTCACGCGG GCTCTGAAGC AGTGGCTGCT 
GCCAGCAGAA TTGGAGCAAA GACACTTCTC ATCACACAGA AAATCCAGAC TATTGGGGCA 
CTCTCGTGTA ACCCATCACT CGGGGGTGTC GGGAAGGGTC ATTTAGTAAG GGAGATTGAC 
GCTCTTGATG GTGTGATGGG ACGGGCTGGA GATTGGGCTG GAATTCATTT TTCCATTCTC 
AACCGCAGCA AGGGACCAGC AGTCTGGGGC CTCAGAGCCC AACTGGACCG TCAGCGATAT 
AAAGACTTTA TGCAGTCAGA GCTTTTATCT ACACCCTTAG TGACCATCCT AGAGGGTTCT 
GTTCAGGACC TTCTGTTGAC TGAGGCTGAT CCAGGGAAAC CAGGAAAACA TAAAGTTTAT 
GGAATATGCC TGGCTAATGG AGGTGGTGAA ATCCATTCCA GCTCTGTGGT TCTGACCACT 
GGCACCTTCC TGTCAGGTGC TCTCTTCATG GGCCAGAACA CTACTCCAGG GGGCCGAATG 
GGGGATCCAC CTTCCTGTGC GGGTCTTTCT CATAACTTAA AAAACGTTTT GGGGTTGAAG 
ATTGGTAGGC TAAGGACTGG TACTCCTCCC CGTATTGTCA AGGATTCAAT TGACTTCTCC 
CTCACCTCTG TTTGTTTGCC TGACTCAAGT CCTACTCCAT TCAGCTTCAT TAACAAACAC 
ACACACTGCA CGCCTAAAGA GCAACTTCCA TGTTTTTTGA CATACACCAC TCCTGGTGTA 
GAAAAAGTGG TGAAAGAGAG CAGCCATCTC AACTCACACA TACAGCAAGA CATTAAAGGA 
CCCAGGTACT GCCCCTCCAT TGAGTCAAAG GTGCTGCGGT TTCCAGGGCG ACAGCATCAA 
GTATGGCTGG AACCAGAGGG TTTGACCTCC TCTCTCATGT ACCCTCAGGG TTTGTCTATG 
ACTATGCCAC TGGAGATGCA GCTCCGACTC ATCAGGGAGA TCCCTGGGCT GCAGCATGCC 
AAAATTCAAA CTCCAGGTTA TGGAGTACAA TATGACTTTG TCTGTCCAAC ACAACTTTTC 
CCATGGCTAC AGTTAAAGAA CACTCAGGGT CTATTCTTGG CTGGGCAAAT AAATGGGACC 
ACTGGCTATG AGGAGGCAGC TGCCCAGGGC TTGTGGGCTG GGGTCAATGC AGGTCGAACA 
TCTCTCTCCA TGTCTCCATT GTCCTTGTCA CGCACACAAA GCTACATCGG AGTTTTAATT 
GATGATCTGG TTTGTCATGG AGTAACAGAG CCATATCGTA TGTTCACAAG CAGAGCTGAG 
TTTAGAACCT CCCTCAGACC AGACAACGCT GACCTGCGAC TAACTATAAG AGGTTTTGAG 
GAGATTGGTT GTGTGTCTGA ACAGCGCTAT AATAAAGCTC TTCTGGTTCA GGCAAGTTTG 
AAGCAGGCTC TATCTACTCT GCAGTCCATC CATCTCTCCG CTGCTCGCTG GAGGGATAAA 
CTGGGAGGTG TGACCATTAG CAAGACAAAG AGTTCCATTC TGAATGGAGT GGAGGTCTTG 
CAGTATAAAG ATGTTACTTT TCAGATGTTG GCATCTTCCT TCCAAGAACT ATTTGGCCCC 
CATCTAGAGA TTTCAGAGAG ATTGAAAGTT GAAGCTCTAT ACCATCCACA CTGTGAGAAG 
CAGAGAAGAG AGATGGAGAA AATGCAAACA GAGGAAAGTT TGTCATTGCC AAATGACATT 
GACTATCTGG CTCTACCCAT CTCGCTTTCT CAGGAGGTCC GAGAGATTCT GGACAGAGTG 
CGACCAAGCA CACTGGGAGC TGCAGCCCGT CTGCCAGGAA TTACTCCAGC AGCTATAGTT 
CATCTGTTCA ACTACGTGCA CAGAAACAAA CACAGAAATC ATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304746.1
CDS	85..2172
Translation	MTAVKPAETQSSQHDIYIEISKNSVTLQVSVPQLSHMLVRRLQFLKTLSTCSIEASNIHTQHYDVIVVGGGHAGSEAVAAASRIGAKTLLITQKIQTIGALSCNPSLGGVGKGHLVREIDALDGVMGRAGDWAGIHFSILNRSKGPAVWGLRAQLDRQRYKDFMQSELLSTPLVTILEGSVQDLLLTEADPGKPGKHKVYGICLANGGGEIHSSSVVLTTGTFLSGALFMGQNTTPGGRMGDPPSCAGLSHNLKNVLGLKIGRLRTGTPPRIVKDSIDFSLTSVCLPDSSPTPFSFINKHTHCTPKEQLPCFLTYTTPGVEKVVKESSHLNSHIQQDIKGPRYCPSIESKVLRFPGRQHQVWLEPEGLTSSLMYPQGLSMTMPLEMQLRLIREIPGLQHAKIQTPGYGVQYDFVCPTQLFPWLQLKNTQGLFLAGQINGTTGYEEAAAQGLWAGVNAGRTSLSMSPLSLSRTQSYIGVLIDDLVCHGVTEPYRMFTSRAEFRTSLRPDNADLRLTIRGFEEIGCVSEQRYNKALLVQASLKQALSTLQSIHLSAARWRDKLGGVTISKTKSSILNGVEVLQYKDVTFQMLASSFQELFGPHLEISERLKVEALYHPHCEKQRREMEKMQTEESLSLPNDIDYLALPISLSQEVREILDRVRPSTLGAAARLPGITPAAIVHLFNYVHRNKHRNHM

Target ORF information:

RefSeq Version	XM_009306471.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306471.2

ORF Insert Sequence:

ATGACTGCAG TCAAACCAGC GGAAACACAG TCAAGCCAGC ACGATATATA TATTGAGATT 
TCAAAAAATT CTGTTACCCT CCAAGTTAGT GTACCACAAT TGTCACACAT GTTGGTAAGA 
AGATTGCAGT TTCTAAAGAC TCTCTCCACC TGCTCTATTG AAGCTTCTAA TATACACACT 
CAACATTATG ATGTAATTGT GGTTGGCGGT GGTCACGCGG GCTCTGAAGC AGTGGCTGCT 
GCCAGCAGAA TTGGAGCAAA GACACTTCTC ATCACACAGA AAATCCAGAC TATTGGGGCA 
CTCTCGTGTA ACCCATCACT CGGGGGTGTC GGGAAGGGTC ATTTAGTAAG GGAGATTGAC 
GCTCTTGATG GTGTGATGGG ACGGGCTGGA GATTGGGCTG GAATTCATTT TTCCATTCTC 
AACCGCAGCA AGGGACCAGC AGTCTGGGGC CTCAGAGCCC AACTGGACCG TCAGCGATAT 
AAAGACTTTA TGCAGTCAGA GCTTTTATCT ACACCCTTAG TGACCATCCT AGAGGGTTCT 
GTTCAGGACC TTCTGTTGAC TGAGGCTGAT CCAGGGAAAC CAGGAAAACA TAAAGTTTAT 
GGAATATGCC TGGCTAATGG AGGTGGTGAA ATCCATTCCA GCTCTGTGGT TCTGACCACT 
GGCACCTTCC TGTCAGGTGC TCTCTTCATG GGCCAGAACA CTACTCCAGG GGGCCGAATG 
GGGGATCCAC CTTCCTGTGC GGGTCTTTCT CATAACTTAA AAAACGTTTT GGGGTTGAAG 
ATTGGTAGGC TAAGGACTGG TACTCCTCCC CGTATTGTCA AGGATTCAAT TGACTTCTCC 
CTCACCTCTG TTTGTTTGCC TGACTCAAGT CCTACTCCAT TCAGCTTCAT TAACAAACAC 
ACACACTGCA CGCCTAAAGA GCAACTTCCA TGTTTTTTGA CATACACCAC TCCTGGTGTA 
GAAAAAGTGG TGAAAGAGAG CAGCCATCTC AACTCACACA TACAGCAAGA CATTAAAGGA 
CCCAGGTACT GCCCCTCCAT TGAGTCAAAG GTGCTGCGGT TTCCAGGGCG ACAGCATCAA 
GTATGGCTGG AACCAGAGGG TTTGACCTCC TCTCTCATGT ACCCTCAGGG TTTGTCTATG 
ACTATGCCAC TGGAGATGCA GCTCCGACTC ATCAGGGAGA TCCCTGGGCT GCAGCATGCC 
AAAATTCAAA CTCCAGGTTA TGGAGTACAA TATGACTTTG TCTGTCCAAC ACAACTTTTC 
CCATGGCTAC AGTTAAAGAA CACTCAGGGT CTATTCTTGG CTGGGCAAAT AAATGGGACC 
ACTGGCTATG AGGAGGCAGC TGCCCAGGGC TTGTGGGCTG GGGTCAATGC AGGTCGAACA 
TCTCTCTCCA TGTCTCCATT GTCCTTGTCA CGCACACAAA GCTACATCGG AGTTTTAATT 
GATGATCTGG TTTGTCATGG AGTAACAGAG CCATATCGTA TGTTCACAAG CAGAGCTGAG 
TTTAGAACCT CCCTCAGACC AGACAACGCT GACCTGCGAC TAACTATAAG AGGTTTTGAG 
GAGATTGGTT GTGTGTCTGA ACAGCGCTAT AATAAAGCTC TTCTGGTTCA GGCAAGTTTG 
AAGCAGGCTC TATCTACTCT GCAGTCCATC CATCTCTCCG CTGCTCGCTG GAGGGATAAA 
CTGGGAGGTG TGACCATTAG CAAGACAAAG AGTTCCATTC TGAATGGAGT GGAGGTCTTG 
CAGTATAAAG ATGTTACTTT TCAGATGTTG GCATCTTCCT TCCAAGAACT ATTTGGCCCC 
CATCTAGAGA TTTCAGAGAG ATTGAAAGTT GAAGCTCTAT ACCATCCACA CTGTGAGAAG 
CAGAGAAGAG AGATGGAGAA AATGCAAACA GAGGAAAGTT TGTCATTGCC AAATGACATT 
GACTATCTGG CTCTACCCAT CTCGCTTTCT CAGGAGGTCC GAGAGATTCT GGACAGAGTG 
CGACCAAGCA CACTGGGAGC TGCAGCCCGT CTGCCAGGAA TTACTCCAGC AGCTATAGTT 
CATCTGTTCA ACTACGTGCA CAGAAACAAA CACAGAAATC ATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTO1 ( XM_009306471.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304746.1 Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48334
Clone ID Related Accession (Same CDS sequence)	XM_009306471.2 , XM_009306471.3
Accession Version	XM_009306471.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein MTO1 homolog, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306471.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCAG TCAAACCAGC GGAAACACAG TCAAGCCAGC ACGATATATA TATTGAGATT 
TCAAAAAATT CTGTTACCCT CCAAGTTAGT GTACCACAAT TGTCACACAT GTTGGTAAGA 
AGATTGCAGT TTCTAAAGAC TCTCTCCACC TGCTCTATTG AAGCTTCTAA TATACACACT 
CAACATTATG ATGTAATTGT GGTTGGCGGT GGTCACGCGG GCTCTGAAGC AGTGGCTGCT 
GCCAGCAGAA TTGGAGCAAA GACACTTCTC ATCACACAGA AAATCCAGAC TATTGGGGCA 
CTCTCGTGTA ACCCATCACT CGGGGGTGTC GGGAAGGGTC ATTTAGTAAG GGAGATTGAC 
GCTCTTGATG GTGTGATGGG ACGGGCTGGA GATTGGGCTG GAATTCATTT TTCCATTCTC 
AACCGCAGCA AGGGACCAGC AGTCTGGGGC CTCAGAGCCC AACTGGACCG TCAGCGATAT 
AAAGACTTTA TGCAGTCAGA GCTTTTATCT ACACCCTTAG TGACCATCCT AGAGGGTTCT 
GTTCAGGACC TTCTGTTGAC TGAGGCTGAT CCAGGGAAAC CAGGAAAACA TAAAGTTTAT 
GGAATATGCC TGGCTAATGG AGGTGGTGAA ATCCATTCCA GCTCTGTGGT TCTGACCACT 
GGCACCTTCC TGTCAGGTGC TCTCTTCATG GGCCAGAACA CTACTCCAGG GGGCCGAATG 
GGGGATCCAC CTTCCTGTGC GGGTCTTTCT CATAACTTAA AAAACGTTTT GGGGTTGAAG 
ATTGGTAGGC TAAGGACTGG TACTCCTCCC CGTATTGTCA AGGATTCAAT TGACTTCTCC 
CTCACCTCTG TTTGTTTGCC TGACTCAAGT CCTACTCCAT TCAGCTTCAT TAACAAACAC 
ACACACTGCA CGCCTAAAGA GCAACTTCCA TGTTTTTTGA CATACACCAC TCCTGGTGTA 
GAAAAAGTGG TGAAAGAGAG CAGCCATCTC AACTCACACA TACAGCAAGA CATTAAAGGA 
CCCAGGTACT GCCCCTCCAT TGAGTCAAAG GTGCTGCGGT TTCCAGGGCG ACAGCATCAA 
GTATGGCTGG AACCAGAGGG TTTGACCTCC TCTCTCATGT ACCCTCAGGG TTTGTCTATG 
ACTATGCCAC TGGAGATGCA GCTCCGACTC ATCAGGGAGA TCCCTGGGCT GCAGCATGCC 
AAAATTCAAA CTCCAGGTTA TGGAGTACAA TATGACTTTG TCTGTCCAAC ACAACTTTTC 
CCATGGCTAC AGTTAAAGAA CACTCAGGGT CTATTCTTGG CTGGGCAAAT AAATGGGACC 
ACTGGCTATG AGGAGGCAGC TGCCCAGGGC TTGTGGGCTG GGGTCAATGC AGGTCGAACA 
TCTCTCTCCA TGTCTCCATT GTCCTTGTCA CGCACACAAA GCTACATCGG AGTTTTAATT 
GATGATCTGG TTTGTCATGG AGTAACAGAG CCATATCGTA TGTTCACAAG CAGAGCTGAG 
TTTAGAACCT CCCTCAGACC AGACAACGCT GACCTGCGAC TAACTATAAG AGGTTTTGAG 
GAGATTGGTT GTGTGTCTGA ACAGCGCTAT AATAAAGCTC TTCTGGTTCA GGCAAGTTTG 
AAGCAGGCTC TATCTACTCT GCAGTCCATC CATCTCTCCG CTGCTCGCTG GAGGGATAAA 
CTGGGAGGTG TGACCATTAG CAAGACAAAG AGTTCCATTC TGAATGGAGT GGAGGTCTTG 
CAGTATAAAG ATGTTACTTT TCAGATGTTG GCATCTTCCT TCCAAGAACT ATTTGGCCCC 
CATCTAGAGA TTTCAGAGAG ATTGAAAGTT GAAGCTCTAT ACCATCCACA CTGTGAGAAG 
CAGAGAAGAG AGATGGAGAA AATGCAAACA GAGGAAAGTT TGTCATTGCC AAATGACATT 
GACTATCTGG CTCTACCCAT CTCGCTTTCT CAGGAGGTCC GAGAGATTCT GGACAGAGTG 
CGACCAAGCA CACTGGGAGC TGCAGCCCGT CTGCCAGGAA TTACTCCAGC AGCTATAGTT 
CATCTGTTCA ACTACGTGCA CAGAAACAAA CACAGAAATC ATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304746.1
CDS	144..2231
Translation	MTAVKPAETQSSQHDIYIEISKNSVTLQVSVPQLSHMLVRRLQFLKTLSTCSIEASNIHTQHYDVIVVGGGHAGSEAVAAASRIGAKTLLITQKIQTIGALSCNPSLGGVGKGHLVREIDALDGVMGRAGDWAGIHFSILNRSKGPAVWGLRAQLDRQRYKDFMQSELLSTPLVTILEGSVQDLLLTEADPGKPGKHKVYGICLANGGGEIHSSSVVLTTGTFLSGALFMGQNTTPGGRMGDPPSCAGLSHNLKNVLGLKIGRLRTGTPPRIVKDSIDFSLTSVCLPDSSPTPFSFINKHTHCTPKEQLPCFLTYTTPGVEKVVKESSHLNSHIQQDIKGPRYCPSIESKVLRFPGRQHQVWLEPEGLTSSLMYPQGLSMTMPLEMQLRLIREIPGLQHAKIQTPGYGVQYDFVCPTQLFPWLQLKNTQGLFLAGQINGTTGYEEAAAQGLWAGVNAGRTSLSMSPLSLSRTQSYIGVLIDDLVCHGVTEPYRMFTSRAEFRTSLRPDNADLRLTIRGFEEIGCVSEQRYNKALLVQASLKQALSTLQSIHLSAARWRDKLGGVTISKTKSSILNGVEVLQYKDVTFQMLASSFQELFGPHLEISERLKVEALYHPHCEKQRREMEKMQTEESLSLPNDIDYLALPISLSQEVREILDRVRPSTLGAAARLPGITPAAIVHLFNYVHRNKHRNHM

Target ORF information:

RefSeq Version	XM_009306471.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306471.3

ORF Insert Sequence:

ATGACTGCAG TCAAACCAGC GGAAACACAG TCAAGCCAGC ACGATATATA TATTGAGATT 
TCAAAAAATT CTGTTACCCT CCAAGTTAGT GTACCACAAT TGTCACACAT GTTGGTAAGA 
AGATTGCAGT TTCTAAAGAC TCTCTCCACC TGCTCTATTG AAGCTTCTAA TATACACACT 
CAACATTATG ATGTAATTGT GGTTGGCGGT GGTCACGCGG GCTCTGAAGC AGTGGCTGCT 
GCCAGCAGAA TTGGAGCAAA GACACTTCTC ATCACACAGA AAATCCAGAC TATTGGGGCA 
CTCTCGTGTA ACCCATCACT CGGGGGTGTC GGGAAGGGTC ATTTAGTAAG GGAGATTGAC 
GCTCTTGATG GTGTGATGGG ACGGGCTGGA GATTGGGCTG GAATTCATTT TTCCATTCTC 
AACCGCAGCA AGGGACCAGC AGTCTGGGGC CTCAGAGCCC AACTGGACCG TCAGCGATAT 
AAAGACTTTA TGCAGTCAGA GCTTTTATCT ACACCCTTAG TGACCATCCT AGAGGGTTCT 
GTTCAGGACC TTCTGTTGAC TGAGGCTGAT CCAGGGAAAC CAGGAAAACA TAAAGTTTAT 
GGAATATGCC TGGCTAATGG AGGTGGTGAA ATCCATTCCA GCTCTGTGGT TCTGACCACT 
GGCACCTTCC TGTCAGGTGC TCTCTTCATG GGCCAGAACA CTACTCCAGG GGGCCGAATG 
GGGGATCCAC CTTCCTGTGC GGGTCTTTCT CATAACTTAA AAAACGTTTT GGGGTTGAAG 
ATTGGTAGGC TAAGGACTGG TACTCCTCCC CGTATTGTCA AGGATTCAAT TGACTTCTCC 
CTCACCTCTG TTTGTTTGCC TGACTCAAGT CCTACTCCAT TCAGCTTCAT TAACAAACAC 
ACACACTGCA CGCCTAAAGA GCAACTTCCA TGTTTTTTGA CATACACCAC TCCTGGTGTA 
GAAAAAGTGG TGAAAGAGAG CAGCCATCTC AACTCACACA TACAGCAAGA CATTAAAGGA 
CCCAGGTACT GCCCCTCCAT TGAGTCAAAG GTGCTGCGGT TTCCAGGGCG ACAGCATCAA 
GTATGGCTGG AACCAGAGGG TTTGACCTCC TCTCTCATGT ACCCTCAGGG TTTGTCTATG 
ACTATGCCAC TGGAGATGCA GCTCCGACTC ATCAGGGAGA TCCCTGGGCT GCAGCATGCC 
AAAATTCAAA CTCCAGGTTA TGGAGTACAA TATGACTTTG TCTGTCCAAC ACAACTTTTC 
CCATGGCTAC AGTTAAAGAA CACTCAGGGT CTATTCTTGG CTGGGCAAAT AAATGGGACC 
ACTGGCTATG AGGAGGCAGC TGCCCAGGGC TTGTGGGCTG GGGTCAATGC AGGTCGAACA 
TCTCTCTCCA TGTCTCCATT GTCCTTGTCA CGCACACAAA GCTACATCGG AGTTTTAATT 
GATGATCTGG TTTGTCATGG AGTAACAGAG CCATATCGTA TGTTCACAAG CAGAGCTGAG 
TTTAGAACCT CCCTCAGACC AGACAACGCT GACCTGCGAC TAACTATAAG AGGTTTTGAG 
GAGATTGGTT GTGTGTCTGA ACAGCGCTAT AATAAAGCTC TTCTGGTTCA GGCAAGTTTG 
AAGCAGGCTC TATCTACTCT GCAGTCCATC CATCTCTCCG CTGCTCGCTG GAGGGATAAA 
CTGGGAGGTG TGACCATTAG CAAGACAAAG AGTTCCATTC TGAATGGAGT GGAGGTCTTG 
CAGTATAAAG ATGTTACTTT TCAGATGTTG GCATCTTCCT TCCAAGAACT ATTTGGCCCC 
CATCTAGAGA TTTCAGAGAG ATTGAAAGTT GAAGCTCTAT ACCATCCACA CTGTGAGAAG 
CAGAGAAGAG AGATGGAGAA AATGCAAACA GAGGAAAGTT TGTCATTGCC AAATGACATT 
GACTATCTGG CTCTACCCAT CTCGCTTTCT CAGGAGGTCC GAGAGATTCT GGACAGAGTG 
CGACCAAGCA CACTGGGAGC TGCAGCCCGT CTGCCAGGAA TTACTCCAGC AGCTATAGTT 
CATCTGTTCA ACTACGTGCA CAGAAACAAA CACAGAAATC ATATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTO1 ( XM_021480003.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021335678.1 Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X3, mRNA.

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CloneID	ODa148125
Clone ID Related Accession (Same CDS sequence)	XM_021480003.1 , XM_021480004.1
Accession Version	XM_021480003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein MTO1 homolog, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGGG CTGGAGATTG GGCTGGAATT CATTTTTCCA TTCTCAACCG CAGCAAGGGA 
CCAGCAGTCT GGGGCCTCAG AGCCCAACTG GACCGTCAGC GATATAAAGA CTTTATGCAG 
TCAGAGCTTT TATCTACACC CTTAGTGACC ATCCTAGAGG GTTCTGTTCA GGACCTTCTG 
TTGACTGAGG CTGATCCAGG GAAACCAGGA AAACATAAAG TTTATGGAAT ATGCCTGGCT 
AATGGAGGTG GTGAAATCCA TTCCAGCTCT GTGGTTCTGA CCACTGGCAC CTTCCTGTCA 
GGTGCTCTCT TCATGGGCCA GAACACTACT CCAGGGGGCC GAATGGGGGA TCCACCTTCC 
TGTGCGGGTC TTTCTCATAA CTTAAAAAAC GTTTTGGGGT TGAAGATTGG TAGGCTAAGG 
ACTGGTACTC CTCCCCGTAT TGTCAAGGAT TCAATTGACT TCTCCCTCAC CTCTGTTTGT 
TTGCCTGACT CAAGTCCTAC TCCATTCAGC TTCATTAACA AACACACACA CTGCACGCCT 
AAAGAGCAAC TTCCATGTTT TTTGACATAC ACCACTCCTG GTGTAGAAAA AGTGGTGAAA 
GAGAGCAGCC ATCTCAACTC ACACATACAG CAAGACATTA AAGGACCCAG GTACTGCCCC 
TCCATTGAGT CAAAGGTGCT GCGGTTTCCA GGGCGACAGC ATCAAGTATG GCTGGAACCA 
GAGGGTTTGA CCTCCTCTCT CATGTACCCT CAGGGTTTGT CTATGACTAT GCCACTGGAG 
ATGCAGCTCC GACTCATCAG GGAGATCCCT GGGCTGCAGC ATGCCAAAAT TCAAACTCCA 
GGTTATGGAG TACAATATGA CTTTGTCTGT CCAACACAAC TTTTCCCATG GCTACAGTTA 
AAGAACACTC AGGGTCTATT CTTGGCTGGG CAAATAAATG GGACCACTGG CTATGAGGAG 
GCAGCTGCCC AGGGCTTGTG GGCTGGGGTC AATGCAGGTC GAACATCTCT CTCCATGTCT 
CCATTGTCCT TGTCACGCAC ACAAAGCTAC ATCGGAGTTT TAATTGATGA TCTGGTTTGT 
CATGGAGTAA CAGAGCCATA TCGTATGTTC ACAAGCAGAG CTGAGTTTAG AACCTCCCTC 
AGACCAGACA ACGCTGACCT GCGACTAACT ATAAGAGGTT TTGAGGAGAT TGGTTGTGTG 
TCTGAACAGC GCTATAATAA AGCTCTTCTG GTTCAGGCAA GTTTGAAGCA GGCTCTATCT 
ACTCTGCAGT CCATCCATCT CTCCGCTGCT CGCTGGAGGG ATAAACTGGG AGGTGTGACC 
ATTAGCAAGA CAAAGAGTTC CATTCTGAAT GGAGTGGAGG TCTTGCAGTA TAAAGATGTT 
ACTTTTCAGA TGTTGGCATC TTCCTTCCAA GAACTATTTG GCCCCCATCT AGAGATTTCA 
GAGAGATTGA AAGTTGAAGC TCTATACCAT CCACACTGTG AGAAGCAGAG AAGAGAGATG 
GAGAAAATGC AAACAGAGGA AAGTTTGTCA TTGCCAAATG ACATTGACTA TCTGGCTCTA 
CCCATCTCGC TTTCTCAGGA GGTCCGAGAG ATTCTGGACA GAGTGCGACC AAGCACACTG 
GGAGCTGCAG CCCGTCTGCC AGGAATTACT CCAGCAGCTA TAGTTCATCT GTTCAACTAC 
GTGCACAGAA ACAAACACAG AAATCATATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335678.1
CDS	357..2069
Translation	MGRAGDWAGIHFSILNRSKGPAVWGLRAQLDRQRYKDFMQSELLSTPLVTILEGSVQDLLLTEADPGKPGKHKVYGICLANGGGEIHSSSVVLTTGTFLSGALFMGQNTTPGGRMGDPPSCAGLSHNLKNVLGLKIGRLRTGTPPRIVKDSIDFSLTSVCLPDSSPTPFSFINKHTHCTPKEQLPCFLTYTTPGVEKVVKESSHLNSHIQQDIKGPRYCPSIESKVLRFPGRQHQVWLEPEGLTSSLMYPQGLSMTMPLEMQLRLIREIPGLQHAKIQTPGYGVQYDFVCPTQLFPWLQLKNTQGLFLAGQINGTTGYEEAAAQGLWAGVNAGRTSLSMSPLSLSRTQSYIGVLIDDLVCHGVTEPYRMFTSRAEFRTSLRPDNADLRLTIRGFEEIGCVSEQRYNKALLVQASLKQALSTLQSIHLSAARWRDKLGGVTISKTKSSILNGVEVLQYKDVTFQMLASSFQELFGPHLEISERLKVEALYHPHCEKQRREMEKMQTEESLSLPNDIDYLALPISLSQEVREILDRVRPSTLGAAARLPGITPAAIVHLFNYVHRNKHRNHM

Target ORF information:

RefSeq Version	XM_021480003.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480003.1

ORF Insert Sequence:

ATGGGACGGG CTGGAGATTG GGCTGGAATT CATTTTTCCA TTCTCAACCG CAGCAAGGGA 
CCAGCAGTCT GGGGCCTCAG AGCCCAACTG GACCGTCAGC GATATAAAGA CTTTATGCAG 
TCAGAGCTTT TATCTACACC CTTAGTGACC ATCCTAGAGG GTTCTGTTCA GGACCTTCTG 
TTGACTGAGG CTGATCCAGG GAAACCAGGA AAACATAAAG TTTATGGAAT ATGCCTGGCT 
AATGGAGGTG GTGAAATCCA TTCCAGCTCT GTGGTTCTGA CCACTGGCAC CTTCCTGTCA 
GGTGCTCTCT TCATGGGCCA GAACACTACT CCAGGGGGCC GAATGGGGGA TCCACCTTCC 
TGTGCGGGTC TTTCTCATAA CTTAAAAAAC GTTTTGGGGT TGAAGATTGG TAGGCTAAGG 
ACTGGTACTC CTCCCCGTAT TGTCAAGGAT TCAATTGACT TCTCCCTCAC CTCTGTTTGT 
TTGCCTGACT CAAGTCCTAC TCCATTCAGC TTCATTAACA AACACACACA CTGCACGCCT 
AAAGAGCAAC TTCCATGTTT TTTGACATAC ACCACTCCTG GTGTAGAAAA AGTGGTGAAA 
GAGAGCAGCC ATCTCAACTC ACACATACAG CAAGACATTA AAGGACCCAG GTACTGCCCC 
TCCATTGAGT CAAAGGTGCT GCGGTTTCCA GGGCGACAGC ATCAAGTATG GCTGGAACCA 
GAGGGTTTGA CCTCCTCTCT CATGTACCCT CAGGGTTTGT CTATGACTAT GCCACTGGAG 
ATGCAGCTCC GACTCATCAG GGAGATCCCT GGGCTGCAGC ATGCCAAAAT TCAAACTCCA 
GGTTATGGAG TACAATATGA CTTTGTCTGT CCAACACAAC TTTTCCCATG GCTACAGTTA 
AAGAACACTC AGGGTCTATT CTTGGCTGGG CAAATAAATG GGACCACTGG CTATGAGGAG 
GCAGCTGCCC AGGGCTTGTG GGCTGGGGTC AATGCAGGTC GAACATCTCT CTCCATGTCT 
CCATTGTCCT TGTCACGCAC ACAAAGCTAC ATCGGAGTTT TAATTGATGA TCTGGTTTGT 
CATGGAGTAA CAGAGCCATA TCGTATGTTC ACAAGCAGAG CTGAGTTTAG AACCTCCCTC 
AGACCAGACA ACGCTGACCT GCGACTAACT ATAAGAGGTT TTGAGGAGAT TGGTTGTGTG 
TCTGAACAGC GCTATAATAA AGCTCTTCTG GTTCAGGCAA GTTTGAAGCA GGCTCTATCT 
ACTCTGCAGT CCATCCATCT CTCCGCTGCT CGCTGGAGGG ATAAACTGGG AGGTGTGACC 
ATTAGCAAGA CAAAGAGTTC CATTCTGAAT GGAGTGGAGG TCTTGCAGTA TAAAGATGTT 
ACTTTTCAGA TGTTGGCATC TTCCTTCCAA GAACTATTTG GCCCCCATCT AGAGATTTCA 
GAGAGATTGA AAGTTGAAGC TCTATACCAT CCACACTGTG AGAAGCAGAG AAGAGAGATG 
GAGAAAATGC AAACAGAGGA AAGTTTGTCA TTGCCAAATG ACATTGACTA TCTGGCTCTA 
CCCATCTCGC TTTCTCAGGA GGTCCGAGAG ATTCTGGACA GAGTGCGACC AAGCACACTG 
GGAGCTGCAG CCCGTCTGCC AGGAATTACT CCAGCAGCTA TAGTTCATCT GTTCAACTAC 
GTGCACAGAA ACAAACACAG AAATCATATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTO1 ( XM_021480004.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021335679.1 Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X4, mRNA.

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CloneID	ODa148125
Clone ID Related Accession (Same CDS sequence)	XM_021480003.1 , XM_021480004.1
Accession Version	XM_021480004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein MTO1 homolog, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGGG CTGGAGATTG GGCTGGAATT CATTTTTCCA TTCTCAACCG CAGCAAGGGA 
CCAGCAGTCT GGGGCCTCAG AGCCCAACTG GACCGTCAGC GATATAAAGA CTTTATGCAG 
TCAGAGCTTT TATCTACACC CTTAGTGACC ATCCTAGAGG GTTCTGTTCA GGACCTTCTG 
TTGACTGAGG CTGATCCAGG GAAACCAGGA AAACATAAAG TTTATGGAAT ATGCCTGGCT 
AATGGAGGTG GTGAAATCCA TTCCAGCTCT GTGGTTCTGA CCACTGGCAC CTTCCTGTCA 
GGTGCTCTCT TCATGGGCCA GAACACTACT CCAGGGGGCC GAATGGGGGA TCCACCTTCC 
TGTGCGGGTC TTTCTCATAA CTTAAAAAAC GTTTTGGGGT TGAAGATTGG TAGGCTAAGG 
ACTGGTACTC CTCCCCGTAT TGTCAAGGAT TCAATTGACT TCTCCCTCAC CTCTGTTTGT 
TTGCCTGACT CAAGTCCTAC TCCATTCAGC TTCATTAACA AACACACACA CTGCACGCCT 
AAAGAGCAAC TTCCATGTTT TTTGACATAC ACCACTCCTG GTGTAGAAAA AGTGGTGAAA 
GAGAGCAGCC ATCTCAACTC ACACATACAG CAAGACATTA AAGGACCCAG GTACTGCCCC 
TCCATTGAGT CAAAGGTGCT GCGGTTTCCA GGGCGACAGC ATCAAGTATG GCTGGAACCA 
GAGGGTTTGA CCTCCTCTCT CATGTACCCT CAGGGTTTGT CTATGACTAT GCCACTGGAG 
ATGCAGCTCC GACTCATCAG GGAGATCCCT GGGCTGCAGC ATGCCAAAAT TCAAACTCCA 
GGTTATGGAG TACAATATGA CTTTGTCTGT CCAACACAAC TTTTCCCATG GCTACAGTTA 
AAGAACACTC AGGGTCTATT CTTGGCTGGG CAAATAAATG GGACCACTGG CTATGAGGAG 
GCAGCTGCCC AGGGCTTGTG GGCTGGGGTC AATGCAGGTC GAACATCTCT CTCCATGTCT 
CCATTGTCCT TGTCACGCAC ACAAAGCTAC ATCGGAGTTT TAATTGATGA TCTGGTTTGT 
CATGGAGTAA CAGAGCCATA TCGTATGTTC ACAAGCAGAG CTGAGTTTAG AACCTCCCTC 
AGACCAGACA ACGCTGACCT GCGACTAACT ATAAGAGGTT TTGAGGAGAT TGGTTGTGTG 
TCTGAACAGC GCTATAATAA AGCTCTTCTG GTTCAGGCAA GTTTGAAGCA GGCTCTATCT 
ACTCTGCAGT CCATCCATCT CTCCGCTGCT CGCTGGAGGG ATAAACTGGG AGGTGTGACC 
ATTAGCAAGA CAAAGAGTTC CATTCTGAAT GGAGTGGAGG TCTTGCAGTA TAAAGATGTT 
ACTTTTCAGA TGTTGGCATC TTCCTTCCAA GAACTATTTG GCCCCCATCT AGAGATTTCA 
GAGAGATTGA AAGTTGAAGC TCTATACCAT CCACACTGTG AGAAGCAGAG AAGAGAGATG 
GAGAAAATGC AAACAGAGGA AAGTTTGTCA TTGCCAAATG ACATTGACTA TCTGGCTCTA 
CCCATCTCGC TTTCTCAGGA GGTCCGAGAG ATTCTGGACA GAGTGCGACC AAGCACACTG 
GGAGCTGCAG CCCGTCTGCC AGGAATTACT CCAGCAGCTA TAGTTCATCT GTTCAACTAC 
GTGCACAGAA ACAAACACAG AAATCATATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335679.1
CDS	230..1942
Translation	MGRAGDWAGIHFSILNRSKGPAVWGLRAQLDRQRYKDFMQSELLSTPLVTILEGSVQDLLLTEADPGKPGKHKVYGICLANGGGEIHSSSVVLTTGTFLSGALFMGQNTTPGGRMGDPPSCAGLSHNLKNVLGLKIGRLRTGTPPRIVKDSIDFSLTSVCLPDSSPTPFSFINKHTHCTPKEQLPCFLTYTTPGVEKVVKESSHLNSHIQQDIKGPRYCPSIESKVLRFPGRQHQVWLEPEGLTSSLMYPQGLSMTMPLEMQLRLIREIPGLQHAKIQTPGYGVQYDFVCPTQLFPWLQLKNTQGLFLAGQINGTTGYEEAAAQGLWAGVNAGRTSLSMSPLSLSRTQSYIGVLIDDLVCHGVTEPYRMFTSRAEFRTSLRPDNADLRLTIRGFEEIGCVSEQRYNKALLVQASLKQALSTLQSIHLSAARWRDKLGGVTISKTKSSILNGVEVLQYKDVTFQMLASSFQELFGPHLEISERLKVEALYHPHCEKQRREMEKMQTEESLSLPNDIDYLALPISLSQEVREILDRVRPSTLGAAARLPGITPAAIVHLFNYVHRNKHRNHM

Target ORF information:

RefSeq Version	XM_021480004.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitochondrial tRNA translation optimization 1 (mto1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480004.1

ORF Insert Sequence:

ATGGGACGGG CTGGAGATTG GGCTGGAATT CATTTTTCCA TTCTCAACCG CAGCAAGGGA 
CCAGCAGTCT GGGGCCTCAG AGCCCAACTG GACCGTCAGC GATATAAAGA CTTTATGCAG 
TCAGAGCTTT TATCTACACC CTTAGTGACC ATCCTAGAGG GTTCTGTTCA GGACCTTCTG 
TTGACTGAGG CTGATCCAGG GAAACCAGGA AAACATAAAG TTTATGGAAT ATGCCTGGCT 
AATGGAGGTG GTGAAATCCA TTCCAGCTCT GTGGTTCTGA CCACTGGCAC CTTCCTGTCA 
GGTGCTCTCT TCATGGGCCA GAACACTACT CCAGGGGGCC GAATGGGGGA TCCACCTTCC 
TGTGCGGGTC TTTCTCATAA CTTAAAAAAC GTTTTGGGGT TGAAGATTGG TAGGCTAAGG 
ACTGGTACTC CTCCCCGTAT TGTCAAGGAT TCAATTGACT TCTCCCTCAC CTCTGTTTGT 
TTGCCTGACT CAAGTCCTAC TCCATTCAGC TTCATTAACA AACACACACA CTGCACGCCT 
AAAGAGCAAC TTCCATGTTT TTTGACATAC ACCACTCCTG GTGTAGAAAA AGTGGTGAAA 
GAGAGCAGCC ATCTCAACTC ACACATACAG CAAGACATTA AAGGACCCAG GTACTGCCCC 
TCCATTGAGT CAAAGGTGCT GCGGTTTCCA GGGCGACAGC ATCAAGTATG GCTGGAACCA 
GAGGGTTTGA CCTCCTCTCT CATGTACCCT CAGGGTTTGT CTATGACTAT GCCACTGGAG 
ATGCAGCTCC GACTCATCAG GGAGATCCCT GGGCTGCAGC ATGCCAAAAT TCAAACTCCA 
GGTTATGGAG TACAATATGA CTTTGTCTGT CCAACACAAC TTTTCCCATG GCTACAGTTA 
AAGAACACTC AGGGTCTATT CTTGGCTGGG CAAATAAATG GGACCACTGG CTATGAGGAG 
GCAGCTGCCC AGGGCTTGTG GGCTGGGGTC AATGCAGGTC GAACATCTCT CTCCATGTCT 
CCATTGTCCT TGTCACGCAC ACAAAGCTAC ATCGGAGTTT TAATTGATGA TCTGGTTTGT 
CATGGAGTAA CAGAGCCATA TCGTATGTTC ACAAGCAGAG CTGAGTTTAG AACCTCCCTC 
AGACCAGACA ACGCTGACCT GCGACTAACT ATAAGAGGTT TTGAGGAGAT TGGTTGTGTG 
TCTGAACAGC GCTATAATAA AGCTCTTCTG GTTCAGGCAA GTTTGAAGCA GGCTCTATCT 
ACTCTGCAGT CCATCCATCT CTCCGCTGCT CGCTGGAGGG ATAAACTGGG AGGTGTGACC 
ATTAGCAAGA CAAAGAGTTC CATTCTGAAT GGAGTGGAGG TCTTGCAGTA TAAAGATGTT 
ACTTTTCAGA TGTTGGCATC TTCCTTCCAA GAACTATTTG GCCCCCATCT AGAGATTTCA 
GAGAGATTGA AAGTTGAAGC TCTATACCAT CCACACTGTG AGAAGCAGAG AAGAGAGATG 
GAGAAAATGC AAACAGAGGA AAGTTTGTCA TTGCCAAATG ACATTGACTA TCTGGCTCTA 
CCCATCTCGC TTTCTCAGGA GGTCCGAGAG ATTCTGGACA GAGTGCGACC AAGCACACTG 
GGAGCTGCAG CCCGTCTGCC AGGAATTACT CCAGCAGCTA TAGTTCATCT GTTCAACTAC 
GTGCACAGAA ACAAACACAG AAATCATATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multiplicity of Buc copies in Atlantic salmon contrasts with loss of the germ cell determinant in primates, rodents and axolotl.
BMC evolutionary biology16(1)232(2016 Oct)
?kugor A,Tveiten H,Johnsen H,Andersen ?

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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MTO1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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