defbl3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	defbl3
Entrez Gene ID	100009621
Full Name	defensin, beta-like 3
Synonyms	db-3
General protein information	Preferred Names defensin, beta-like 3 Names beta-defensin-like 3 BD-3 defensin-like protein 3 zfdb-3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291435.2	XP_009289710.1	beta-defensin-like 3 isoform X1
NM_001081555.1	NP_001075024.1	beta-defensin-like 3 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Pathogen invasion changes the intestinal microbiota composition and induces innate immune responses in the zebrafish intestine.
Yang HT, Zou SS, Zhai LJ, Wang Y, Zhang FM, An LG, Yang GW
Fish & shellfish immunology7135-42(2017 Dec)

Zebrafish fed on recombinant Artemia expressing epinecidin-1 exhibit increased survival and altered expression of immunomodulatory genes upon Vibrio vulnificus infection.
Jheng YH, Lee LH, Ting CH, Pan CY, Hui CF, Chen JY
Fish & shellfish immunology42(1)1-15(2015 Jan)

Role of intestinal inflammation in predisposition of Edwardsiella tarda infection in zebrafish (Danio rerio).
Liu X, Chang X, Wu H, Xiao J, Gao Y, Zhang Y
Fish & shellfish immunology41(2)271-8(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following defbl3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the defbl3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35911	NM_001081555.1 Latest version!	Danio rerio defensin, beta-like 3 (defbl3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa48327	XM_009291435.2 Latest version!	Danio rerio defensin, beta-like 3 (defbl3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

defbl3 ( NM_001081555.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001075024.1 Danio rerio defensin, beta-like 3 (defbl3), mRNA.

Price & Availability↑

CloneID	ODa35911
Clone ID Related Accession (Same CDS sequence)	NM_001081555.1
Accession Version	NM_001081555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	beta-defensin-like 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AM181360.1. ##Evidence-Data-START## Transcript exon combination :: AM181360.1, DN862371.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505379, SAMEA3505381 [ECO:0000350] ##Evidence-Data-END##

ATGAGCTACA ATACGGGAAC TAACATGAGG ACTCTGGGAC TGATTATTTT TGCTCTGCTG 
CTGCTGACAG CCAGCCAAGC CAATGATACA GACGTGCAGA GATGGACATG CGGATACCGA 
GGACTATGCA GGAAGCACTG CTATGCACGG GAGTATATGA TTGGTTATCG TGGCTGCCCT 
CGCAGATACA GGTGCTGTGC TTTGCGATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075024.1
CDS	1..213
Translation	MSYNTGTNMRTLGLIIFALLLLTASQANDTDVQRWTCGYRGLCRKHCYAREYMIGYRGCPRRYRCCALRF

Target ORF information:

RefSeq Version	NM_001081555.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio defensin, beta-like 3 (defbl3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081555.1

ORF Insert Sequence:

ATGAGCTACA ATACGGGAAC TAACATGAGG ACTCTGGGAC TGATTATTTT TGCTCTGCTG 
CTGCTGACAG CCAGCCAAGC CAATGATACA GACGTGCAGA GATGGACATG CGGATACCGA 
GGACTATGCA GGAAGCACTG CTATGCACGG GAGTATATGA TTGGTTATCG TGGCTGCCCT 
CGCAGATACA GGTGCTGTGC TTTGCGATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

defbl3 ( XM_009291435.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289710.1 Danio rerio defensin, beta-like 3 (defbl3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48327
Clone ID Related Accession (Same CDS sequence)	XM_009291435.2
Accession Version	XM_009291435.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	beta-defensin-like 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291435.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACA ATACGGGAAC TAACATGAGG ACTCTGGGAC TGATTATTTT TGCTCTGCTG 
CTGCTGACAG CCAGCCAAGC CAATGATACA GACGTGCAGA GATGGACATG CGGATACCGA 
GGGCTATGCA GGAAGCACTG CTATGCACGG GAGTATATGA TTGGTTATCG AGGCTGCCCT 
CGCAGATACA GGTACACAAA CGTCCTTCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289710.1
CDS	684..896
Translation	MSYNTGTNMRTLGLIIFALLLLTASQANDTDVQRWTCGYRGLCRKHCYAREYMIGYRGCPRRYRYTNVLQ

Target ORF information:

RefSeq Version	XM_009291435.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio defensin, beta-like 3 (defbl3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291435.2

ORF Insert Sequence:

ATGAGCTACA ATACGGGAAC TAACATGAGG ACTCTGGGAC TGATTATTTT TGCTCTGCTG 
CTGCTGACAG CCAGCCAAGC CAATGATACA GACGTGCAGA GATGGACATG CGGATACCGA 
GGGCTATGCA GGAAGCACTG CTATGCACGG GAGTATATGA TTGGTTATCG AGGCTGCCCT 
CGCAGATACA GGTACACAAA CGTCCTTCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pathogen invasion changes the intestinal microbiota composition and induces innate immune responses in the zebrafish intestine.
Fish & shellfish immunology7135-42(2017 Dec)
Yang HT,Zou SS,Zhai LJ,Wang Y,Zhang FM,An LG,Yang GW

Zebrafish fed on recombinant Artemia expressing epinecidin-1 exhibit increased survival and altered expression of immunomodulatory genes upon Vibrio vulnificus infection.
Fish & shellfish immunology42(1)1-15(2015 Jan)
Jheng YH,Lee LH,Ting CH,Pan CY,Hui CF,Chen JY

Role of intestinal inflammation in predisposition of Edwardsiella tarda infection in zebrafish (Danio rerio).
Fish & shellfish immunology41(2)271-8(2014 Dec)
Liu X,Chang X,Wu H,Xiao J,Gao Y,Zhang Y