prkcba cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prkcba
Entrez Gene ID	100006675
Full Name	protein kinase C, beta a
Synonyms	Pkc53E,prkcb1l,zgc:64063
General protein information	Preferred Names protein kinase C, beta a Names protein kinase C, beta a protein kinase C, beta 1, like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005170844.3	XP_005170901.1	protein kinase C, beta a isoform X1
NM_201029.1	NP_957323.1	protein kinase C, beta a
XM_005170844.4	XP_005170901.1	protein kinase C, beta a isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Phylogeny and distribution of protein kinase C variants in the zebrafish.
Haug MF, Gesemann M, Berger M, Neuhauss SCF
The Journal of comparative neurology(2018 Jan)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Bugel SM, Wehmas LC, La Du JK, Tanguay RL
Toxicology and applied pharmacology29631-41(2016 Apr)

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
Sato Y, Hashiguchi Y, Nishida M
BMC evolutionary biology9127(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prkcba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prkcba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32101	NM_201029.1 Latest version!	Danio rerio protein kinase C, beta a (prkcba), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa05406	XM_005170844.4 Latest version!	Danio rerio protein kinase C, beta a (prkcba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa05406	XM_005170844.3	Danio rerio protein kinase C, beta a (prkcba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prkcba ( NM_201029.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957323.1 Danio rerio protein kinase C, beta a (prkcba), mRNA.

Price & Availability↑

CloneID	ODa32101
Clone ID Related Accession (Same CDS sequence)	NM_201029.1
Accession Version	NM_201029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	protein kinase C, beta a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC054603.1. On Jan 25, 2008 this sequence version replaced XM_001345318.1. ##Evidence-Data-START## Transcript exon combination :: BC054603.1, BC171680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACCGAGT CATCGGATTC AGATGGCAAG GAGGGCGCGC GGGGATTCGC CAGGCAGGGC 
GCGCTGAGGC AGAAGAACGT GCACGAAGTG AAGGACCACA AGTTCATCGC GCGCTTCTTC 
AAGCAGCCGA CCTTCTGCAG CCACTGCACC GACTTCATCT GGGGTTTCGG CAAACAAGGC 
TTCCAGTGTC AAGTGTGCTG TTTTGTGGTT CACAAACGCT GCCATGAGTT TGTTCACTTC 
TCCTGCCCAG GGTCCGACAA GGGCCCTGCA TCAGATGACC CTCGCAAATA TCATTCGTTC 
AAGGTACACA CATACACCAG TCCCACATTC TGTGACCACT GTGGATCTCT ACTGTACGGC 
CTCATACACC AAGGGATGAG ATGTTTGAAT TGTCTGATGA ATATTCATAA GCGATGCTTT 
AAGCATGTTC CCAGCCTGTG TGGAACAGAC CACACTGAGA AGAGAGGGAG GCTGCATTTC 
TCTGCTAGTA TCACAGGCAA TGTTCTCAGT GTGTCCGTCA AGGAGGCTCG TAACTTGGTG 
CCAATGGATC CAAGCGGCCT GTCAGATCCA TACGTGAAGC TCAAATTAAT TCCAGACCCA 
AAGAGCGAGA GCAAGCAGAA GACCAAAACC ATAAAGGGCT GCCTCAATCC GGTCTGGAAC 
GAAGACTTCA CCTTTGCCCT CAAAGACACA GATAAAGACA GGCGTCTGTA TGTGGAGGTG 
TGGGATTGGG ATCGGGGCAC CAGGAATGAC TACATGGGCT CCATGTCTTT TGGCATCTCT 
GAGCTGATGA ACCAAAGTGT GGACGGATGG TTTAAGATTC TCGCTTGTGA GGAAGGGGTC 
TTTTTCAACG TTCCTGTTTA TGTAGATGAA GAGGAGGGCA ACAAGGAGCT GAGTAAGAGA 
TTTGAGAGAG CCAAGATTGG CCCGGGCACT AATAATACAG ATGGCGCTTC AAAAAATGAC 
CTCTCCAAAT ATGATGCCAA TGGCAACCAA GACCGCATGA AGCTCGCAGA CTTCAACTTC 
ATCATGGTGC TCGGCAAAGG CAGCTTTGGC AAGGTGATGC TTGCGGAGCG TAAAGGTTCA 
GACGAGCTGT ACGCAGTGAA GATCTTGAAG AAGGACGTGG TGATTCAGGA TGATGATGTG 
GAGTGCACCA TGGTGGAAAA GAGAGTTCTG GCTTTGTCTG GAAAACCCCC GTTCCTCACG 
CATCTCCATT CCTGCTTTCA GACGATGGAC CGTCTTTACT TCGTGATGGA GTATATCAGC 
GGTGGAGACC TGATGTTTCA GATACAACAA ACGGGAAAGT TTAAGGAGCC CCTCGCTGTG 
TTCTATGCTG GAGAGATTGC CATTGGCCTG TTCTTTCTTC ATTCTAAGGG CATTATTTAC 
AGAGATCTGA AGCTGGATAA TGTGATGTTG GACTCTGAAG GCCACATTAA GATTGCAGAT 
TTTGGCATGT GCAAAGAGAA CATGTTTGAT AAAGACACAA CAAAAACCTT CTGTGGCACT 
CCAGATTACA TCGCCCCAGA GATCATTGCA TATCAGCCGT ATGGAAAGTC TGTGGATTGG 
TGGGCTTATG GAGTTCTCCT GTATGAAATG CTGGCAGGAC AGCCTCCTTT TGATGGTGAG 
GATGAAGATG AGCTGTTCCA GTCCATCATG GAGCATCACG TGTCTTACCC CAAATCCATG 
TCCAAAGAAG CAGTCGCCAT ATGCAAGGGG CTGATGACCA AGCATCCCGG TAAACGTCTG 
GGCTGTGGAC TGGAGGGCGA ACGGGACATT AAAGAGCATG CTTTCTTCCG TTTCGTGGAC 
TGGGAAAGAC TGCAAAACAA GGAAGTTCAA CCTCCATACA AACCCAAATC TTGCGGTCGA 
GACGCAGGGA ACTTTGATCT TTTTTTCACT CGTCATCCTC CAATCCTGAC CCCTCCTGAC 
CTGGAAGTGA TTCAAAACCT GGACCAAGAG GAATTTCGAG GCTTCTCATA CGTTAATACA 
AAATTTGCAT CGATGAAATC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957323.1
CDS	117..2123
Translation	MTESSDSDGKEGARGFARQGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVHFSCPGSDKGPASDDPRKYHSFKVHTYTSPTFCDHCGSLLYGLIHQGMRCLNCLMNIHKRCFKHVPSLCGTDHTEKRGRLHFSASITGNVLSVSVKEARNLVPMDPSGLSDPYVKLKLIPDPKSESKQKTKTIKGCLNPVWNEDFTFALKDTDKDRRLYVEVWDWDRGTRNDYMGSMSFGISELMNQSVDGWFKILACEEGVFFNVPVYVDEEEGNKELSKRFERAKIGPGTNNTDGASKNDLSKYDANGNQDRMKLADFNFIMVLGKGSFGKVMLAERKGSDELYAVKILKKDVVIQDDDVECTMVEKRVLALSGKPPFLTHLHSCFQTMDRLYFVMEYISGGDLMFQIQQTGKFKEPLAVFYAGEIAIGLFFLHSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENMFDKDTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHHVSYPKSMSKEAVAICKGLMTKHPGKRLGCGLEGERDIKEHAFFRFVDWERLQNKEVQPPYKPKSCGRDAGNFDLFFTRHPPILTPPDLEVIQNLDQEEFRGFSYVNTKFASMKSK

Target ORF information:

RefSeq Version	NM_201029.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase C, beta a (prkcba), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201029.1

ORF Insert Sequence:

ATGACCGAGT CATCGGATTC AGATGGCAAG GAGGGCGCGC GGGGATTCGC CAGGCAGGGC 
GCGCTGAGGC AGAAGAACGT GCACGAAGTG AAGGACCACA AGTTCATCGC GCGCTTCTTC 
AAGCAGCCGA CCTTCTGCAG CCACTGCACC GACTTCATCT GGGGTTTCGG CAAACAAGGC 
TTCCAGTGTC AAGTGTGCTG TTTTGTGGTT CACAAACGCT GCCATGAGTT TGTTCACTTC 
TCCTGCCCAG GGTCCGACAA GGGCCCTGCA TCAGATGACC CTCGCAAATA TCATTCGTTC 
AAGGTACACA CATACACCAG TCCCACATTC TGTGACCACT GTGGATCTCT ACTGTACGGC 
CTCATACACC AAGGGATGAG ATGTTTGAAT TGTCTGATGA ATATTCATAA GCGATGCTTT 
AAGCATGTTC CCAGCCTGTG TGGAACAGAC CACACTGAGA AGAGAGGGAG GCTGCATTTC 
TCTGCTAGTA TCACAGGCAA TGTTCTCAGT GTGTCCGTCA AGGAGGCTCG TAACTTGGTG 
CCAATGGATC CAAGCGGCCT GTCAGATCCA TACGTGAAGC TCAAATTAAT TCCAGACCCA 
AAGAGCGAGA GCAAGCAGAA GACCAAAACC ATAAAGGGCT GCCTCAATCC GGTCTGGAAC 
GAAGACTTCA CCTTTGCCCT CAAAGACACA GATAAAGACA GGCGTCTGTA TGTGGAGGTG 
TGGGATTGGG ATCGGGGCAC CAGGAATGAC TACATGGGCT CCATGTCTTT TGGCATCTCT 
GAGCTGATGA ACCAAAGTGT GGACGGATGG TTTAAGATTC TCGCTTGTGA GGAAGGGGTC 
TTTTTCAACG TTCCTGTTTA TGTAGATGAA GAGGAGGGCA ACAAGGAGCT GAGTAAGAGA 
TTTGAGAGAG CCAAGATTGG CCCGGGCACT AATAATACAG ATGGCGCTTC AAAAAATGAC 
CTCTCCAAAT ATGATGCCAA TGGCAACCAA GACCGCATGA AGCTCGCAGA CTTCAACTTC 
ATCATGGTGC TCGGCAAAGG CAGCTTTGGC AAGGTGATGC TTGCGGAGCG TAAAGGTTCA 
GACGAGCTGT ACGCAGTGAA GATCTTGAAG AAGGACGTGG TGATTCAGGA TGATGATGTG 
GAGTGCACCA TGGTGGAAAA GAGAGTTCTG GCTTTGTCTG GAAAACCCCC GTTCCTCACG 
CATCTCCATT CCTGCTTTCA GACGATGGAC CGTCTTTACT TCGTGATGGA GTATATCAGC 
GGTGGAGACC TGATGTTTCA GATACAACAA ACGGGAAAGT TTAAGGAGCC CCTCGCTGTG 
TTCTATGCTG GAGAGATTGC CATTGGCCTG TTCTTTCTTC ATTCTAAGGG CATTATTTAC 
AGAGATCTGA AGCTGGATAA TGTGATGTTG GACTCTGAAG GCCACATTAA GATTGCAGAT 
TTTGGCATGT GCAAAGAGAA CATGTTTGAT AAAGACACAA CAAAAACCTT CTGTGGCACT 
CCAGATTACA TCGCCCCAGA GATCATTGCA TATCAGCCGT ATGGAAAGTC TGTGGATTGG 
TGGGCTTATG GAGTTCTCCT GTATGAAATG CTGGCAGGAC AGCCTCCTTT TGATGGTGAG 
GATGAAGATG AGCTGTTCCA GTCCATCATG GAGCATCACG TGTCTTACCC CAAATCCATG 
TCCAAAGAAG CAGTCGCCAT ATGCAAGGGG CTGATGACCA AGCATCCCGG TAAACGTCTG 
GGCTGTGGAC TGGAGGGCGA ACGGGACATT AAAGAGCATG CTTTCTTCCG TTTCGTGGAC 
TGGGAAAGAC TGCAAAACAA GGAAGTTCAA CCTCCATACA AACCCAAATC TTGCGGTCGA 
GACGCAGGGA ACTTTGATCT TTTTTTCACT CGTCATCCTC CAATCCTGAC CCCTCCTGAC 
CTGGAAGTGA TTCAAAACCT GGACCAAGAG GAATTTCGAG GCTTCTCATA CGTTAATACA 
AAATTTGCAT CGATGAAATC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkcba ( XM_005170844.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170901.1 Danio rerio protein kinase C, beta a (prkcba), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05406
Clone ID Related Accession (Same CDS sequence)	XM_005170844.3 , XM_005170844.4
Accession Version	XM_005170844.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein kinase C, beta a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170844.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGAGT CATCGGATTC AGATGGCAAG GAGGGCGCGC GGGGATTCGC CAGGCAGGGC 
GCGCTGAGGC AGAAGAACGT GCACGAAGTG AAGGATCACA AGTTCATCGC GCGCTTCTTC 
AAGCAGCCGA CCTTCTGCAG CCACTGCACC GACTTCATCT GGGGTTTCGG CAAACAAGGC 
TTCCAGTGTC AAGTGTGCTG TTTTGTGGTT CACAAACGCT GCCATGAGTT TGTTCACTTC 
TCCTGCCCAG GGTCCGACAA GGGCCCTGCA TCAGATGACC CTCGCAAATA TCATTCGTTC 
AAGGTACACA CATACACCAG TCCCACATTC TGTGACCACT GTGGATCTCT ACTGTACGGC 
CTCATACACC AAGGGATGAG ATGTTTGAAT TGTCTGATGA ATATTCATAA GCGATGCTTT 
AAGCATGTTC CCAGCCTGTG TGGAACAGAC CACACTGAGA AGAGAGGGAG GCTGCATTTC 
TCTGCTAGTA TCACAGGCAA TGTTCTCAGT GTGTCCGTCA AGGAGGCTCG TAACTTGGTG 
CCAATGGATC CAAGCGGCCT GTCAGATCCA TACGTGAAGC TCAAATTAAT TCCAGACCCA 
AAGAGCGAGA GCAAGCAGAA GACCAAAACC ATAAAGGGCT GCCTCAATCC GGTCTGGAAC 
GAAGACTTCA CCTTTGCCCT CAAAGACACG GATAAAGACA GGCGCCTGTA TGTGGAGGTG 
TGGGATTGGG ATCGGGGCAC CAGGAATGAC TACATGGGCT CCATGTCTTT TGGCATCTCT 
GAGCTGATGA ACCAAAGTGT GGACGGATGG TTTAAGATTC TCGCTTGTGA GGAAGGGGTC 
TTTTTCAACG TTCCTGTTTA TGTAGATGAA GAGGAGGGCA ACAAGGAGCT GAGTAAGAGA 
TTTGAGAGAG CCAAGATTGG CCCGGGCACT AATAATACAG ATGGCGCTTC AAAAAATGAC 
CTCTCCAAAT ATGATGCCAA TGGCAACCAA GACCGCATGA AGCTCGCAGA CTTCAACTTC 
ATCATGGTGC TCGGCAAAGG CAGCTTTGGC AAGGTGATGC TTGCGGAGCG TAAAGGTTCA 
GACGAGCTGT ACGCAGTGAA GATCTTGAAG AAGGACGTGG TGATTCAGGA TGATGATGTG 
GAGTGCACCA TGGTGGAGAA GAGAGTTCTG GCTTTGTCTG GAAAACCACC GTTCCTCACG 
CATCTCCACT CCTGCTTTCA GACGATGGAC CGTCTTTACT TCGTGATGGA GTATATCAGC 
GGTGGAGACC TGATGTTTCA GATACAACAA ACGGGAAAGT TTAAGGAGCC CCTCGCTGTG 
TTCTATGCTG GAGAGATTGC CATTGGCCTG TTCTTTCTTC ATTCTAAGGG CATTATTTAC 
AGAGATCTGA AGCTGGATAA TGTGATGTTG GACTCTGAAG GCCACATTAA GATTGCAGAT 
TTTGGCATGT GCAAAGAGAA CATGTTTGAT AAAGACACAA CAAAAACCTT CTGTGGCACT 
CCAGATTACA TCGCCCCAGA GATCATTGCA TATCAGCCGT ATGGAAAGTC TGTTGATTGG 
TGGGCTTATG GAGTTCTCCT GTATGAAATG CTGGCAGGAC AGCCTCCTTT TGATGGTGAG 
GATGAAGATG AGCTGTTCCA GTCCATCATG GAGCATCACG TGTCTTACCC CAAATCCATG 
TCCAAAGAAG CAGTCGCCAT ATGCAAGGGG CTGATGACCA AGCATCCCGG TAAACGTCTG 
GGCTGTGGAC TGGAGGGTGA ACGGGACATT AAAGAGCATG CTTTCTTCCG TTTCGTGGAC 
TGGGAAAGAC TGCAAAACAA GGAAGTTCAA CCTCCATACA AACCCAAATC TAAAAATCGC 
CACGACACGG GCAACTTCGA CAAGGAGTTC ACCAAGATGG CGGTTGAGTT GACGCCAACC 
GATAAACTCT TCATCATGAA TCTGGACCAA AACGAATTCC AAGGTTTCTC CTACACCAAC 
CCTGAGTTTG TCATTCAGGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170901.1
CDS	158..2161
Translation	MTESSDSDGKEGARGFARQGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVHFSCPGSDKGPASDDPRKYHSFKVHTYTSPTFCDHCGSLLYGLIHQGMRCLNCLMNIHKRCFKHVPSLCGTDHTEKRGRLHFSASITGNVLSVSVKEARNLVPMDPSGLSDPYVKLKLIPDPKSESKQKTKTIKGCLNPVWNEDFTFALKDTDKDRRLYVEVWDWDRGTRNDYMGSMSFGISELMNQSVDGWFKILACEEGVFFNVPVYVDEEEGNKELSKRFERAKIGPGTNNTDGASKNDLSKYDANGNQDRMKLADFNFIMVLGKGSFGKVMLAERKGSDELYAVKILKKDVVIQDDDVECTMVEKRVLALSGKPPFLTHLHSCFQTMDRLYFVMEYISGGDLMFQIQQTGKFKEPLAVFYAGEIAIGLFFLHSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENMFDKDTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHHVSYPKSMSKEAVAICKGLMTKHPGKRLGCGLEGERDIKEHAFFRFVDWERLQNKEVQPPYKPKSKNRHDTGNFDKEFTKMAVELTPTDKLFIMNLDQNEFQGFSYTNPEFVIQV

Target ORF information:

RefSeq Version	XM_005170844.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein kinase C, beta a (prkcba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170844.3

ORF Insert Sequence:

ATGACCGAGT CATCGGATTC AGATGGCAAG GAGGGCGCGC GGGGATTCGC CAGGCAGGGC 
GCGCTGAGGC AGAAGAACGT GCACGAAGTG AAGGATCACA AGTTCATCGC GCGCTTCTTC 
AAGCAGCCGA CCTTCTGCAG CCACTGCACC GACTTCATCT GGGGTTTCGG CAAACAAGGC 
TTCCAGTGTC AAGTGTGCTG TTTTGTGGTT CACAAACGCT GCCATGAGTT TGTTCACTTC 
TCCTGCCCAG GGTCCGACAA GGGCCCTGCA TCAGATGACC CTCGCAAATA TCATTCGTTC 
AAGGTACACA CATACACCAG TCCCACATTC TGTGACCACT GTGGATCTCT ACTGTACGGC 
CTCATACACC AAGGGATGAG ATGTTTGAAT TGTCTGATGA ATATTCATAA GCGATGCTTT 
AAGCATGTTC CCAGCCTGTG TGGAACAGAC CACACTGAGA AGAGAGGGAG GCTGCATTTC 
TCTGCTAGTA TCACAGGCAA TGTTCTCAGT GTGTCCGTCA AGGAGGCTCG TAACTTGGTG 
CCAATGGATC CAAGCGGCCT GTCAGATCCA TACGTGAAGC TCAAATTAAT TCCAGACCCA 
AAGAGCGAGA GCAAGCAGAA GACCAAAACC ATAAAGGGCT GCCTCAATCC GGTCTGGAAC 
GAAGACTTCA CCTTTGCCCT CAAAGACACG GATAAAGACA GGCGCCTGTA TGTGGAGGTG 
TGGGATTGGG ATCGGGGCAC CAGGAATGAC TACATGGGCT CCATGTCTTT TGGCATCTCT 
GAGCTGATGA ACCAAAGTGT GGACGGATGG TTTAAGATTC TCGCTTGTGA GGAAGGGGTC 
TTTTTCAACG TTCCTGTTTA TGTAGATGAA GAGGAGGGCA ACAAGGAGCT GAGTAAGAGA 
TTTGAGAGAG CCAAGATTGG CCCGGGCACT AATAATACAG ATGGCGCTTC AAAAAATGAC 
CTCTCCAAAT ATGATGCCAA TGGCAACCAA GACCGCATGA AGCTCGCAGA CTTCAACTTC 
ATCATGGTGC TCGGCAAAGG CAGCTTTGGC AAGGTGATGC TTGCGGAGCG TAAAGGTTCA 
GACGAGCTGT ACGCAGTGAA GATCTTGAAG AAGGACGTGG TGATTCAGGA TGATGATGTG 
GAGTGCACCA TGGTGGAGAA GAGAGTTCTG GCTTTGTCTG GAAAACCACC GTTCCTCACG 
CATCTCCACT CCTGCTTTCA GACGATGGAC CGTCTTTACT TCGTGATGGA GTATATCAGC 
GGTGGAGACC TGATGTTTCA GATACAACAA ACGGGAAAGT TTAAGGAGCC CCTCGCTGTG 
TTCTATGCTG GAGAGATTGC CATTGGCCTG TTCTTTCTTC ATTCTAAGGG CATTATTTAC 
AGAGATCTGA AGCTGGATAA TGTGATGTTG GACTCTGAAG GCCACATTAA GATTGCAGAT 
TTTGGCATGT GCAAAGAGAA CATGTTTGAT AAAGACACAA CAAAAACCTT CTGTGGCACT 
CCAGATTACA TCGCCCCAGA GATCATTGCA TATCAGCCGT ATGGAAAGTC TGTTGATTGG 
TGGGCTTATG GAGTTCTCCT GTATGAAATG CTGGCAGGAC AGCCTCCTTT TGATGGTGAG 
GATGAAGATG AGCTGTTCCA GTCCATCATG GAGCATCACG TGTCTTACCC CAAATCCATG 
TCCAAAGAAG CAGTCGCCAT ATGCAAGGGG CTGATGACCA AGCATCCCGG TAAACGTCTG 
GGCTGTGGAC TGGAGGGTGA ACGGGACATT AAAGAGCATG CTTTCTTCCG TTTCGTGGAC 
TGGGAAAGAC TGCAAAACAA GGAAGTTCAA CCTCCATACA AACCCAAATC TAAAAATCGC 
CACGACACGG GCAACTTCGA CAAGGAGTTC ACCAAGATGG CGGTTGAGTT GACGCCAACC 
GATAAACTCT TCATCATGAA TCTGGACCAA AACGAATTCC AAGGTTTCTC CTACACCAAC 
CCTGAGTTTG TCATTCAGGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkcba ( XM_005170844.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170901.1 Danio rerio protein kinase C, beta a (prkcba), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05406
Clone ID Related Accession (Same CDS sequence)	XM_005170844.3 , XM_005170844.4
Accession Version	XM_005170844.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein kinase C, beta a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170844.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGAGT CATCGGATTC AGATGGCAAG GAGGGCGCGC GGGGATTCGC CAGGCAGGGC 
GCGCTGAGGC AGAAGAACGT GCACGAAGTG AAGGATCACA AGTTCATCGC GCGCTTCTTC 
AAGCAGCCGA CCTTCTGCAG CCACTGCACC GACTTCATCT GGGGTTTCGG CAAACAAGGC 
TTCCAGTGTC AAGTGTGCTG TTTTGTGGTT CACAAACGCT GCCATGAGTT TGTTCACTTC 
TCCTGCCCAG GGTCCGACAA GGGCCCTGCA TCAGATGACC CTCGCAAATA TCATTCGTTC 
AAGGTACACA CATACACCAG TCCCACATTC TGTGACCACT GTGGATCTCT ACTGTACGGC 
CTCATACACC AAGGGATGAG ATGTTTGAAT TGTCTGATGA ATATTCATAA GCGATGCTTT 
AAGCATGTTC CCAGCCTGTG TGGAACAGAC CACACTGAGA AGAGAGGGAG GCTGCATTTC 
TCTGCTAGTA TCACAGGCAA TGTTCTCAGT GTGTCCGTCA AGGAGGCTCG TAACTTGGTG 
CCAATGGATC CAAGCGGCCT GTCAGATCCA TACGTGAAGC TCAAATTAAT TCCAGACCCA 
AAGAGCGAGA GCAAGCAGAA GACCAAAACC ATAAAGGGCT GCCTCAATCC GGTCTGGAAC 
GAAGACTTCA CCTTTGCCCT CAAAGACACG GATAAAGACA GGCGCCTGTA TGTGGAGGTG 
TGGGATTGGG ATCGGGGCAC CAGGAATGAC TACATGGGCT CCATGTCTTT TGGCATCTCT 
GAGCTGATGA ACCAAAGTGT GGACGGATGG TTTAAGATTC TCGCTTGTGA GGAAGGGGTC 
TTTTTCAACG TTCCTGTTTA TGTAGATGAA GAGGAGGGCA ACAAGGAGCT GAGTAAGAGA 
TTTGAGAGAG CCAAGATTGG CCCGGGCACT AATAATACAG ATGGCGCTTC AAAAAATGAC 
CTCTCCAAAT ATGATGCCAA TGGCAACCAA GACCGCATGA AGCTCGCAGA CTTCAACTTC 
ATCATGGTGC TCGGCAAAGG CAGCTTTGGC AAGGTGATGC TTGCGGAGCG TAAAGGTTCA 
GACGAGCTGT ACGCAGTGAA GATCTTGAAG AAGGACGTGG TGATTCAGGA TGATGATGTG 
GAGTGCACCA TGGTGGAGAA GAGAGTTCTG GCTTTGTCTG GAAAACCACC GTTCCTCACG 
CATCTCCACT CCTGCTTTCA GACGATGGAC CGTCTTTACT TCGTGATGGA GTATATCAGC 
GGTGGAGACC TGATGTTTCA GATACAACAA ACGGGAAAGT TTAAGGAGCC CCTCGCTGTG 
TTCTATGCTG GAGAGATTGC CATTGGCCTG TTCTTTCTTC ATTCTAAGGG CATTATTTAC 
AGAGATCTGA AGCTGGATAA TGTGATGTTG GACTCTGAAG GCCACATTAA GATTGCAGAT 
TTTGGCATGT GCAAAGAGAA CATGTTTGAT AAAGACACAA CAAAAACCTT CTGTGGCACT 
CCAGATTACA TCGCCCCAGA GATCATTGCA TATCAGCCGT ATGGAAAGTC TGTTGATTGG 
TGGGCTTATG GAGTTCTCCT GTATGAAATG CTGGCAGGAC AGCCTCCTTT TGATGGTGAG 
GATGAAGATG AGCTGTTCCA GTCCATCATG GAGCATCACG TGTCTTACCC CAAATCCATG 
TCCAAAGAAG CAGTCGCCAT ATGCAAGGGG CTGATGACCA AGCATCCCGG TAAACGTCTG 
GGCTGTGGAC TGGAGGGTGA ACGGGACATT AAAGAGCATG CTTTCTTCCG TTTCGTGGAC 
TGGGAAAGAC TGCAAAACAA GGAAGTTCAA CCTCCATACA AACCCAAATC TAAAAATCGC 
CACGACACGG GCAACTTCGA CAAGGAGTTC ACCAAGATGG CGGTTGAGTT GACGCCAACC 
GATAAACTCT TCATCATGAA TCTGGACCAA AACGAATTCC AAGGTTTCTC CTACACCAAC 
CCTGAGTTTG TCATTCAGGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170901.1
CDS	162..2165
Translation	MTESSDSDGKEGARGFARQGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVHFSCPGSDKGPASDDPRKYHSFKVHTYTSPTFCDHCGSLLYGLIHQGMRCLNCLMNIHKRCFKHVPSLCGTDHTEKRGRLHFSASITGNVLSVSVKEARNLVPMDPSGLSDPYVKLKLIPDPKSESKQKTKTIKGCLNPVWNEDFTFALKDTDKDRRLYVEVWDWDRGTRNDYMGSMSFGISELMNQSVDGWFKILACEEGVFFNVPVYVDEEEGNKELSKRFERAKIGPGTNNTDGASKNDLSKYDANGNQDRMKLADFNFIMVLGKGSFGKVMLAERKGSDELYAVKILKKDVVIQDDDVECTMVEKRVLALSGKPPFLTHLHSCFQTMDRLYFVMEYISGGDLMFQIQQTGKFKEPLAVFYAGEIAIGLFFLHSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENMFDKDTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHHVSYPKSMSKEAVAICKGLMTKHPGKRLGCGLEGERDIKEHAFFRFVDWERLQNKEVQPPYKPKSKNRHDTGNFDKEFTKMAVELTPTDKLFIMNLDQNEFQGFSYTNPEFVIQV

Target ORF information:

RefSeq Version	XM_005170844.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase C, beta a (prkcba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170844.4

ORF Insert Sequence:

ATGACCGAGT CATCGGATTC AGATGGCAAG GAGGGCGCGC GGGGATTCGC CAGGCAGGGC 
GCGCTGAGGC AGAAGAACGT GCACGAAGTG AAGGATCACA AGTTCATCGC GCGCTTCTTC 
AAGCAGCCGA CCTTCTGCAG CCACTGCACC GACTTCATCT GGGGTTTCGG CAAACAAGGC 
TTCCAGTGTC AAGTGTGCTG TTTTGTGGTT CACAAACGCT GCCATGAGTT TGTTCACTTC 
TCCTGCCCAG GGTCCGACAA GGGCCCTGCA TCAGATGACC CTCGCAAATA TCATTCGTTC 
AAGGTACACA CATACACCAG TCCCACATTC TGTGACCACT GTGGATCTCT ACTGTACGGC 
CTCATACACC AAGGGATGAG ATGTTTGAAT TGTCTGATGA ATATTCATAA GCGATGCTTT 
AAGCATGTTC CCAGCCTGTG TGGAACAGAC CACACTGAGA AGAGAGGGAG GCTGCATTTC 
TCTGCTAGTA TCACAGGCAA TGTTCTCAGT GTGTCCGTCA AGGAGGCTCG TAACTTGGTG 
CCAATGGATC CAAGCGGCCT GTCAGATCCA TACGTGAAGC TCAAATTAAT TCCAGACCCA 
AAGAGCGAGA GCAAGCAGAA GACCAAAACC ATAAAGGGCT GCCTCAATCC GGTCTGGAAC 
GAAGACTTCA CCTTTGCCCT CAAAGACACG GATAAAGACA GGCGCCTGTA TGTGGAGGTG 
TGGGATTGGG ATCGGGGCAC CAGGAATGAC TACATGGGCT CCATGTCTTT TGGCATCTCT 
GAGCTGATGA ACCAAAGTGT GGACGGATGG TTTAAGATTC TCGCTTGTGA GGAAGGGGTC 
TTTTTCAACG TTCCTGTTTA TGTAGATGAA GAGGAGGGCA ACAAGGAGCT GAGTAAGAGA 
TTTGAGAGAG CCAAGATTGG CCCGGGCACT AATAATACAG ATGGCGCTTC AAAAAATGAC 
CTCTCCAAAT ATGATGCCAA TGGCAACCAA GACCGCATGA AGCTCGCAGA CTTCAACTTC 
ATCATGGTGC TCGGCAAAGG CAGCTTTGGC AAGGTGATGC TTGCGGAGCG TAAAGGTTCA 
GACGAGCTGT ACGCAGTGAA GATCTTGAAG AAGGACGTGG TGATTCAGGA TGATGATGTG 
GAGTGCACCA TGGTGGAGAA GAGAGTTCTG GCTTTGTCTG GAAAACCACC GTTCCTCACG 
CATCTCCACT CCTGCTTTCA GACGATGGAC CGTCTTTACT TCGTGATGGA GTATATCAGC 
GGTGGAGACC TGATGTTTCA GATACAACAA ACGGGAAAGT TTAAGGAGCC CCTCGCTGTG 
TTCTATGCTG GAGAGATTGC CATTGGCCTG TTCTTTCTTC ATTCTAAGGG CATTATTTAC 
AGAGATCTGA AGCTGGATAA TGTGATGTTG GACTCTGAAG GCCACATTAA GATTGCAGAT 
TTTGGCATGT GCAAAGAGAA CATGTTTGAT AAAGACACAA CAAAAACCTT CTGTGGCACT 
CCAGATTACA TCGCCCCAGA GATCATTGCA TATCAGCCGT ATGGAAAGTC TGTTGATTGG 
TGGGCTTATG GAGTTCTCCT GTATGAAATG CTGGCAGGAC AGCCTCCTTT TGATGGTGAG 
GATGAAGATG AGCTGTTCCA GTCCATCATG GAGCATCACG TGTCTTACCC CAAATCCATG 
TCCAAAGAAG CAGTCGCCAT ATGCAAGGGG CTGATGACCA AGCATCCCGG TAAACGTCTG 
GGCTGTGGAC TGGAGGGTGA ACGGGACATT AAAGAGCATG CTTTCTTCCG TTTCGTGGAC 
TGGGAAAGAC TGCAAAACAA GGAAGTTCAA CCTCCATACA AACCCAAATC TAAAAATCGC 
CACGACACGG GCAACTTCGA CAAGGAGTTC ACCAAGATGG CGGTTGAGTT GACGCCAACC 
GATAAACTCT TCATCATGAA TCTGGACCAA AACGAATTCC AAGGTTTCTC CTACACCAAC 
CCTGAGTTTG TCATTCAGGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phylogeny and distribution of protein kinase C variants in the zebrafish.
The Journal of comparative neurology(2018 Jan)
Haug MF,Gesemann M,Berger M,Neuhauss SCF

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Toxicology and applied pharmacology29631-41(2016 Apr)
Bugel SM,Wehmas LC,La Du JK,Tanguay RL

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
BMC evolutionary biology9127(2009 Jun)
Sato Y,Hashiguchi Y,Nishida M

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prkcba cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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