col27a1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	col27a1a
Entrez Gene ID	100004688
Full Name	collagen, type XXVII, alpha 1a
Synonyms	sb:cb221
General protein information	Preferred Names collagen, type XXVII, alpha 1a Names collagen alpha-1(XXVII) chain A cb221 collagen, type XXVII, alpha 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001163294.1	NP_001156766.1	collagen alpha-1(XXVII) chain A precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	col27a1b	NP_001074044.1
Danio rerio (zebrafish)	col27a1a	NP_001156766.1

Gene Ontology
Bibliography

Related articles in PubMed

Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish.
Wehner D, Tsarouchas TM, Michael A, Haase C, Weidinger G, Reimer MM, Becker T, Becker CG
Nature communications8(1)126(2017 07)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Collagen duplicate genes of bone and cartilage participate during regeneration of zebrafish fin skeleton.
Duran I, Csukasi F, Taylor SP, Krakow D, Becerra J, Bombarely A, Mar?-Beffa M
Gene expression patterns : GEP19(1-2)60-9(2015 Sep-Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings indicate a key role for type XXVII collagen in notochord morphogenesis and axial skeletogenesis.
Title: Critical early roles for col27a1a and col27a1b in zebrafish notochord morphogenesis, vertebral mineralization and post-embryonic axial growth.

The following col27a1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col27a1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01455	NM_001163294.1 Latest version!	Danio rerio collagen, type XXVII, alpha 1a (col27a1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col27a1a ( NM_001163294.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001156766.1 Danio rerio collagen, type XXVII, alpha 1a (col27a1a), mRNA.

Price & Availability↑

CloneID	ODa01455
Clone ID Related Accession (Same CDS sequence)	NM_001163294.1
Accession Version	NM_001163294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XXVII) chain A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ229459.1. On Jul 17, 2009 this sequence version replaced XM_001343896.2. ##Evidence-Data-START## Transcript exon combination :: GQ229459.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476741, SAMEA4476744 [ECO:0000348] ##Evidence-Data-END##

ATGAATTTAG CGACCCGAAG GAGAGTCCGC AGAACCTCTC GTCTGGTGGC AAAGAGAGCT 
CTTCTTCTGT GTATTCTACT TTATTGTACT TCTTTTGGAT TCACCCAAGT GCTCTTTGAA 
GATGTGGACG TGTTGCAGCG GTTGGCTCTG CGTGCTGAGC TCGGGTCTCG TGCCGTTCCC 
GCTGGCGTCA TCTCCCTCCG CTCAGGAGTC ATCCTCACCA CACGGGCACG GGTCACCACA 
CCCACACGCT CCCTGTTCCC ACCAGAACTC TTCTGGAACT GCACTATCAT CCTGAGCGTC 
CGATCCCACC GCTTAAACTC AGCCTTTCTC TTCTCCGTCC TATCTGGGAA TCGGATTCAG 
CTTGGGTTGG AAATTTCCCC TGGCAAGCTA ACACTTCATG CAGGCCCCGG GAATGCTGCG 
ACGTTCCTAT ACAACCTTCA CGATGGACGC TGGCACCATC TAGCCTTTGT TATAAATGGG 
CGATCAGTGA CCCTACACTC TCCCTGCTCG GAGAGTGACA GCGGAGTTAC TCAGGAGCTC 
CCTGTACTTC CCGAACGCCT GAACCCTCGA GGAACCTTCC GCCTGGGGGG CAGCAGTGCT 
CTGCTTCCGG GTGTGGTGCC CTTTGAGGGG GCGGTTTGCC AGTTTGATGT GGTGCCCTCA 
GCACAAGCAC AGCAAAATAT CTGCAGCGCC ATTCGCAGGC AGTGCAGAGA AAATGACACC 
TACAGGCCGG CACCTCCTGC CCTGCTTCCA CTCCCATCTC GACACGCCCC ACCACTATTG 
GCCCACACCT TACCACCTAA TCGCACTTTC ACTTTCACTC CCACTAATTT CCTTTTGGCA 
CCCGTGAATG GCGCAGGAGG GTCTTCTTCT GTGAGAATGA GTGATGGTGT GCGACCCAAG 
CCTTCCTCCA CCACACCTCC ACCTCTTGCC TTGATGCAAA CAGGAATTGA TGCTCCTTTA 
TCTTTAAGTT TGGTCACCCA TAAGCCTAGT CTCCGGACAC CCAAGCCCAC TGCCTCAAAA 
CCTGGAGTCT GGCTTACTCC CACAAAACCA GCCAGACCAA AACCCACTCC AGGAAAAGCA 
TCTCCAAAAC TTAACGTATC TAAATCTTTT GGACCAAAGC CCACAGCACG ACTGGCTGCT 
TCCAAACTTG GTTCAAAGGC AATTGGTCCC AAGCCTACAC CATTGAAACC CTCCAAACCT 
GTAAAGAAGC CGACATCTGT GCCAAAGCCC AACCCTACGA AGAATGCGTC TATTGGTCCC 
AGACCTACCA ATTCAAACAA GAAACAAAAC GCCATTCTGA AACCCTTGCC TGCCCCCAAA 
CCGACTGTCC CCAAAAGACC CTCGCCAACC AACAAAAAGC CTCTCCAGCC CAAAAACAAA 
TCCCACACCA CGCCGTTAAC CCCCAAATCC ACCCTGGCTC CAAACTCTAC ATCTAAAAAG 
CCCCTGCCGA CCCTAAAATC CACTTCGTTC ACCACTGCTG CACCAAACAA ACCCCCCAAA 
ACTCTGGAAA CCCCAAAAGT CAATCCTGAC AAGTCTAAAA CGCCCGTGCC TTATTCCACA 
CCACGCACTC CAAGGTTCTC CATACAATCT GTGACTCTTC CGGCGTTTGA CGATTTCCAG 
AGCTTCGAAG TAGAGCCCAC GCGCTTTTCA CTTCTGGTTG GAGCACCAGG ACTAAAAGGA 
GATCAAGGGG AATCGGGATT ACCGGGACCT CCAGGTAAAC CAGGGCAGCC CGGGATGCGA 
GGACCGCGGG GACCTCCTGG ACCACATGGC AAGCCAGGTC GACCCGGTCC TACAGGACTG 
AAGGGGAAGA AGGGAGACCC CGGACTCTCT CCTGGAAAAG CACCAAAAGG AGATAAAGGA 
GATGTTGGTC TGCCCGGTCC TGTTGGTTTA GTCGGGGTGG AAGGCCGTAA GGGACAAAAA 
GGCCATCCGG GACCACCAGG CCTTCCCGGG GAACCAGGAG AACAGGGTCC TGTTGGAGAA 
GCTGGAGCCA AAGGCTATCC TGGCAGGCAG GGTTTACCTG GTCCAATAGG GCCTGTTGGG 
CCGAAAGGTG CTCGGGGTTT CATAGGAATC CCAGGACTTT TCGGTTTACC TGGAGCTGAT 
GGTGAAAGAG GCAGTCCTGG TCCACCAGGG AAGAGGGGCA AGATGGGTAG GCCGGGTTTT 
CCTGGTGATT TCGGGGAGCG AGGGCCGCCA GGACTGGATG GGGAACCAGG TGTTATTGGA 
GCTCCTGGTC CTCCTGGTGT CCTCGGCTTG ATTGGTGATA TGGGCCCCGC TGGGACAGTC 
GGTGTACCAG GACTAAATGG GCTGAAAGGC GTCCCGGGGA ACATGGGCGA GTCCGGACTG 
AAAGGTGATA AGGGTGACGT TGGGCTTCCT GGAGAACAAG GAGAGATTGG CTTCCAAGGA 
GACAAGGGTG TGCAGGGTTT ACCGGGACTA CCAGGACCTC GTGGAAAACC AGGACCACAG 
GGCAAGACAG GAGAAATAGG GCCCAGTGGA TTACCAGGCC CACCAGGACC CGAGGGTTTT 
CCTGGTGATA TTGGTAAACC GGGTCTCAAT GGACCTGAGG GGCCAAAGGG TAAACCTGGA 
GCCAGAGGTT TACCGGGACC TCGTGGAGCC GCTGGACGAG AGGGTGACGA GGGTCCTCTA 
GGACCACCAG GACCATTCGG ACTCGAGGGT CAGATGGGCA GTAAGGGTTT CCCTGGGGCT 
CTTGGACTTG AAGGGGTCAA AGGTGAACAG GGAGTCACAG GGAAAGCTGG ACCCATGGGT 
GAGAGGGGTC TCGTTGGCTT TATCGGTCCC GGAGGAGAGG CTGGACTCGC TGGAGAAAAA 
GGTGATCGCG GAGAGATGGG TTTGCCGGGA CCTCCAGGAG AGAAAGGATC TACGGGTCAC 
CCAGGAACTC CGGGAGAAGG CGGCCCACCA GGACCACCAG GCAGTCCAGG CTCACCGGGA 
TCACGTGGAC CCATCGGCAT AAGAGGACCT AAAGGAAGAC GGGGTCCTAG AGGTCCAGAT 
GGGGTGCCAG GTGAGATTGG CACTGAAGGC AAAAAGGGTC CTGATGGTCC TCCTGGAAAA 
ATTGGTTTCC CTGGACACGC GGGAAAGATC GGCGAGTCTG GGGAGGTGGG GCCAAAGGGA 
TTTCCGGGGA TTCAAGGGCC ATCCGGGGCT ACAGGAGACA AAGGAATCGC AGGGGAGCCA 
GGACCGTCAG GACCTCCAGG AACACTCGGG CCTCAAGGAA ATCCCGGACC AAAGGGCCCT 
GCAGGAAAAG TTGGTGATTC TGGACTTCCA GGAGAACCTG GAGAAAAGGG GTCAATCGGG 
CTTGCAGGGA ATGCTGGGGC TGCAGGACTT ATCGGAGCAA GGGGAGAACC AGGTTTAGAG 
GGAGAGGCTG GTCCCGCTGG TCCTGATGGC ACAAAGGGTG AAAAGGGTGA CATGGGCACA 
GAAGGTGAGC AGGGGGTCAG AGGTGACCCT GGAATTAAGG GGAAAGACGG GCCACCGGGC 
GACCCTGGAC TTACTGGAGT CAGAGGTCCT GAAGGAAAAT CTGGTAAAAG TGGCGAGAGA 
GGGAAACCAG GGCTTAAGGG TGCCAAAGGT AACATCGGTC ATCTGGGCGA GACGGGAAGT 
GTGGGAAAGA TCGGGCCAAT CGGAACCACT GGACCAAAGG GCTCTCGAGG AACTATCGGA 
CATGCGGGTG CTCCTGGGCG TATGGGCTTG CAGGGCGATC CAGGTATATC AGGATATGAG 
GGTCACAAAG GACCTCAAGG TCCAATTGGA CCCCCGGGAC CAAAGGGTGC AAAGGGTGAA 
CAGGGGGATG ATGGGAAGGT GGAGGGTCCG ACCGGTGCAC CGGGTCTCAG AGGTCCCGTT 
GGAAAGCGGG GAGACAGGGG TGAGCCAGGA GATCCAGGAT ATGTGGGTCA GCAAGGTGTT 
GATGGTTTAC GTGGAAAACC TGGAGCTCCT GGACTGCCTG GCGATCCTGG TCCACGTGGA 
ACACAAGGCC CTAAGGGATC CAAAGGAGAA CAGGGTCAGA AGGGCAAACA GGGTCAGCAG 
GGAGAGCGTG GCAGCAGAGG ATCACCGGGT GTTGTTGGGT TGCCAGGTCC TCGAGGTACT 
GTGGGACGAG AAGGACGAGA GGGTTTTCCT GGTACAGATG GACTTGCTGG AAAGGATGGA 
AGCAGAGGAA CACCTGGCGA TCAGGGAGAT GATGGAGAGT TCGGTTTGCC TGGTAAACCT 
GGTGCACCTG GAAAAGTTGG CGTCATCGGT TTACCAGGCC CACAGGGGTC ATTCGGTCCA 
AAGGGTGAGC GAGGTCTCCC TGGTCACCCC GGCCCTTCAG GAAAGAGAGG ATTTAAGGGA 
GGAATGGGTC TTCCTGGACC TCAGGGAGAC CGGGGATCTA AAGGCCAACC TGGTGACATC 
GGGGAACCCG GCTTTCCAGG AATGCTGGGA ATGTTTGGAC CAAAGGGGCC TCCTGGAGAC 
TTTGGACCAA AGGGGATTCA GGGACCCAAA GGACCCCAAG GCAACATGGG CAGAGGTGGT 
TTAGCTGGAC CTGTCGGTGT GATCGGCCCT ATTGGTAATC CAGGCTCAAG AGGAGATACA 
GGCAACAAAG GAGAGCTGGG TGTTCAGGGT CCTCGAGGTG CTCCTGGACC ACGTGGACCT 
CCTGGACTTC CGGGTCCTCC GGGAATCCCG CTGGCCATGA ATCAGGATTT TGGTCTCGGT 
GTAGTTCAGC CAGTTTTCCG AGAGACTCAT ACACAGAAAA TTGAGGGTCG GGGTCTACTG 
GACATGCCCA TGTTGGATCA GGCTCCAGAG ATCCTGCGAA CGCTTGATTA TCTGAGCTCT 
CTAGTGCACA GCCTGAAGAA CCCACTGGGG ACACGAGACC ATCCCGCTCG ACTCTGCAGA 
GACCTGCATG ACTGCCGGGA CACGCTTTAC GATGGCACTT ACTGGATCGA CCCAAACCTG 
GGCTGCTCCT CAGACTCTAT AGAAGTGATG TGTAATTTCA GCAGTGGCGG ACGCACGTGT 
CTCAGACCCA TCACCACGGC CAAGCTGGAG TTCAGTGTTG GACGGGTTCA GATGAACTTT 
CTGCACCTGC TGAGCGCCGG AGCGGAGCAA CGAATCACTA TCCACTGCTT GAACGTGACT 
ATCTGGAGCC ACGCCCCCAA CCAGCCTCCT TCCCAAAATG CAGTTCAGTT CCACTCATGG 
ATCGGAGAGG TGCTGGAGCC GGATGTGTTG GAGGACACCT GCTGGCAATT GAACGGGCGC 
TGGCAGCACG CCGACTTCCT GTTCCGGGTG TTGGACCCTG CCTTACTCCC TGTCGTACGC 
ATAAGCAATC TCCCAAAGGT GATGCCCTCA TCACGCTTTC ACCTTGAAGT CGGACCTGTG 
TGCTTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156766.1
CDS	1..5352
Translation	MNLATRRRVRRTSRLVAKRALLLCILLYCTSFGFTQVLFEDVDVLQRLALRAELGSRAVPAGVISLRSGVILTTRARVTTPTRSLFPPELFWNCTIILSVRSHRLNSAFLFSVLSGNRIQLGLEISPGKLTLHAGPGNAATFLYNLHDGRWHHLAFVINGRSVTLHSPCSESDSGVTQELPVLPERLNPRGTFRLGGSSALLPGVVPFEGAVCQFDVVPSAQAQQNICSAIRRQCRENDTYRPAPPALLPLPSRHAPPLLAHTLPPNRTFTFTPTNFLLAPVNGAGGSSSVRMSDGVRPKPSSTTPPPLALMQTGIDAPLSLSLVTHKPSLRTPKPTASKPGVWLTPTKPARPKPTPGKASPKLNVSKSFGPKPTARLAASKLGSKAIGPKPTPLKPSKPVKKPTSVPKPNPTKNASIGPRPTNSNKKQNAILKPLPAPKPTVPKRPSPTNKKPLQPKNKSHTTPLTPKSTLAPNSTSKKPLPTLKSTSFTTAAPNKPPKTLETPKVNPDKSKTPVPYSTPRTPRFSIQSVTLPAFDDFQSFEVEPTRFSLLVGAPGLKGDQGESGLPGPPGKPGQPGMRGPRGPPGPHGKPGRPGPTGLKGKKGDPGLSPGKAPKGDKGDVGLPGPVGLVGVEGRKGQKGHPGPPGLPGEPGEQGPVGEAGAKGYPGRQGLPGPIGPVGPKGARGFIGIPGLFGLPGADGERGSPGPPGKRGKMGRPGFPGDFGERGPPGLDGEPGVIGAPGPPGVLGLIGDMGPAGTVGVPGLNGLKGVPGNMGESGLKGDKGDVGLPGEQGEIGFQGDKGVQGLPGLPGPRGKPGPQGKTGEIGPSGLPGPPGPEGFPGDIGKPGLNGPEGPKGKPGARGLPGPRGAAGREGDEGPLGPPGPFGLEGQMGSKGFPGALGLEGVKGEQGVTGKAGPMGERGLVGFIGPGGEAGLAGEKGDRGEMGLPGPPGEKGSTGHPGTPGEGGPPGPPGSPGSPGSRGPIGIRGPKGRRGPRGPDGVPGEIGTEGKKGPDGPPGKIGFPGHAGKIGESGEVGPKGFPGIQGPSGATGDKGIAGEPGPSGPPGTLGPQGNPGPKGPAGKVGDSGLPGEPGEKGSIGLAGNAGAAGLIGARGEPGLEGEAGPAGPDGTKGEKGDMGTEGEQGVRGDPGIKGKDGPPGDPGLTGVRGPEGKSGKSGERGKPGLKGAKGNIGHLGETGSVGKIGPIGTTGPKGSRGTIGHAGAPGRMGLQGDPGISGYEGHKGPQGPIGPPGPKGAKGEQGDDGKVEGPTGAPGLRGPVGKRGDRGEPGDPGYVGQQGVDGLRGKPGAPGLPGDPGPRGTQGPKGSKGEQGQKGKQGQQGERGSRGSPGVVGLPGPRGTVGREGREGFPGTDGLAGKDGSRGTPGDQGDDGEFGLPGKPGAPGKVGVIGLPGPQGSFGPKGERGLPGHPGPSGKRGFKGGMGLPGPQGDRGSKGQPGDIGEPGFPGMLGMFGPKGPPGDFGPKGIQGPKGPQGNMGRGGLAGPVGVIGPIGNPGSRGDTGNKGELGVQGPRGAPGPRGPPGLPGPPGIPLAMNQDFGLGVVQPVFRETHTQKIEGRGLLDMPMLDQAPEILRTLDYLSSLVHSLKNPLGTRDHPARLCRDLHDCRDTLYDGTYWIDPNLGCSSDSIEVMCNFSSGGRTCLRPITTAKLEFSVGRVQMNFLHLLSAGAEQRITIHCLNVTIWSHAPNQPPSQNAVQFHSWIGEVLEPDVLEDTCWQLNGRWQHADFLFRVLDPALLPVVRISNLPKVMPSSRFHLEVGPVCFL

Target ORF information:

RefSeq Version	NM_001163294.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XXVII, alpha 1a (col27a1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163294.1

ORF Insert Sequence:

ATGAATTTAG CGACCCGAAG GAGAGTCCGC AGAACCTCTC GTCTGGTGGC AAAGAGAGCT 
CTTCTTCTGT GTATTCTACT TTATTGTACT TCTTTTGGAT TCACCCAAGT GCTCTTTGAA 
GATGTGGACG TGTTGCAGCG GTTGGCTCTG CGTGCTGAGC TCGGGTCTCG TGCCGTTCCC 
GCTGGCGTCA TCTCCCTCCG CTCAGGAGTC ATCCTCACCA CACGGGCACG GGTCACCACA 
CCCACACGCT CCCTGTTCCC ACCAGAACTC TTCTGGAACT GCACTATCAT CCTGAGCGTC 
CGATCCCACC GCTTAAACTC AGCCTTTCTC TTCTCCGTCC TATCTGGGAA TCGGATTCAG 
CTTGGGTTGG AAATTTCCCC TGGCAAGCTA ACACTTCATG CAGGCCCCGG GAATGCTGCG 
ACGTTCCTAT ACAACCTTCA CGATGGACGC TGGCACCATC TAGCCTTTGT TATAAATGGG 
CGATCAGTGA CCCTACACTC TCCCTGCTCG GAGAGTGACA GCGGAGTTAC TCAGGAGCTC 
CCTGTACTTC CCGAACGCCT GAACCCTCGA GGAACCTTCC GCCTGGGGGG CAGCAGTGCT 
CTGCTTCCGG GTGTGGTGCC CTTTGAGGGG GCGGTTTGCC AGTTTGATGT GGTGCCCTCA 
GCACAAGCAC AGCAAAATAT CTGCAGCGCC ATTCGCAGGC AGTGCAGAGA AAATGACACC 
TACAGGCCGG CACCTCCTGC CCTGCTTCCA CTCCCATCTC GACACGCCCC ACCACTATTG 
GCCCACACCT TACCACCTAA TCGCACTTTC ACTTTCACTC CCACTAATTT CCTTTTGGCA 
CCCGTGAATG GCGCAGGAGG GTCTTCTTCT GTGAGAATGA GTGATGGTGT GCGACCCAAG 
CCTTCCTCCA CCACACCTCC ACCTCTTGCC TTGATGCAAA CAGGAATTGA TGCTCCTTTA 
TCTTTAAGTT TGGTCACCCA TAAGCCTAGT CTCCGGACAC CCAAGCCCAC TGCCTCAAAA 
CCTGGAGTCT GGCTTACTCC CACAAAACCA GCCAGACCAA AACCCACTCC AGGAAAAGCA 
TCTCCAAAAC TTAACGTATC TAAATCTTTT GGACCAAAGC CCACAGCACG ACTGGCTGCT 
TCCAAACTTG GTTCAAAGGC AATTGGTCCC AAGCCTACAC CATTGAAACC CTCCAAACCT 
GTAAAGAAGC CGACATCTGT GCCAAAGCCC AACCCTACGA AGAATGCGTC TATTGGTCCC 
AGACCTACCA ATTCAAACAA GAAACAAAAC GCCATTCTGA AACCCTTGCC TGCCCCCAAA 
CCGACTGTCC CCAAAAGACC CTCGCCAACC AACAAAAAGC CTCTCCAGCC CAAAAACAAA 
TCCCACACCA CGCCGTTAAC CCCCAAATCC ACCCTGGCTC CAAACTCTAC ATCTAAAAAG 
CCCCTGCCGA CCCTAAAATC CACTTCGTTC ACCACTGCTG CACCAAACAA ACCCCCCAAA 
ACTCTGGAAA CCCCAAAAGT CAATCCTGAC AAGTCTAAAA CGCCCGTGCC TTATTCCACA 
CCACGCACTC CAAGGTTCTC CATACAATCT GTGACTCTTC CGGCGTTTGA CGATTTCCAG 
AGCTTCGAAG TAGAGCCCAC GCGCTTTTCA CTTCTGGTTG GAGCACCAGG ACTAAAAGGA 
GATCAAGGGG AATCGGGATT ACCGGGACCT CCAGGTAAAC CAGGGCAGCC CGGGATGCGA 
GGACCGCGGG GACCTCCTGG ACCACATGGC AAGCCAGGTC GACCCGGTCC TACAGGACTG 
AAGGGGAAGA AGGGAGACCC CGGACTCTCT CCTGGAAAAG CACCAAAAGG AGATAAAGGA 
GATGTTGGTC TGCCCGGTCC TGTTGGTTTA GTCGGGGTGG AAGGCCGTAA GGGACAAAAA 
GGCCATCCGG GACCACCAGG CCTTCCCGGG GAACCAGGAG AACAGGGTCC TGTTGGAGAA 
GCTGGAGCCA AAGGCTATCC TGGCAGGCAG GGTTTACCTG GTCCAATAGG GCCTGTTGGG 
CCGAAAGGTG CTCGGGGTTT CATAGGAATC CCAGGACTTT TCGGTTTACC TGGAGCTGAT 
GGTGAAAGAG GCAGTCCTGG TCCACCAGGG AAGAGGGGCA AGATGGGTAG GCCGGGTTTT 
CCTGGTGATT TCGGGGAGCG AGGGCCGCCA GGACTGGATG GGGAACCAGG TGTTATTGGA 
GCTCCTGGTC CTCCTGGTGT CCTCGGCTTG ATTGGTGATA TGGGCCCCGC TGGGACAGTC 
GGTGTACCAG GACTAAATGG GCTGAAAGGC GTCCCGGGGA ACATGGGCGA GTCCGGACTG 
AAAGGTGATA AGGGTGACGT TGGGCTTCCT GGAGAACAAG GAGAGATTGG CTTCCAAGGA 
GACAAGGGTG TGCAGGGTTT ACCGGGACTA CCAGGACCTC GTGGAAAACC AGGACCACAG 
GGCAAGACAG GAGAAATAGG GCCCAGTGGA TTACCAGGCC CACCAGGACC CGAGGGTTTT 
CCTGGTGATA TTGGTAAACC GGGTCTCAAT GGACCTGAGG GGCCAAAGGG TAAACCTGGA 
GCCAGAGGTT TACCGGGACC TCGTGGAGCC GCTGGACGAG AGGGTGACGA GGGTCCTCTA 
GGACCACCAG GACCATTCGG ACTCGAGGGT CAGATGGGCA GTAAGGGTTT CCCTGGGGCT 
CTTGGACTTG AAGGGGTCAA AGGTGAACAG GGAGTCACAG GGAAAGCTGG ACCCATGGGT 
GAGAGGGGTC TCGTTGGCTT TATCGGTCCC GGAGGAGAGG CTGGACTCGC TGGAGAAAAA 
GGTGATCGCG GAGAGATGGG TTTGCCGGGA CCTCCAGGAG AGAAAGGATC TACGGGTCAC 
CCAGGAACTC CGGGAGAAGG CGGCCCACCA GGACCACCAG GCAGTCCAGG CTCACCGGGA 
TCACGTGGAC CCATCGGCAT AAGAGGACCT AAAGGAAGAC GGGGTCCTAG AGGTCCAGAT 
GGGGTGCCAG GTGAGATTGG CACTGAAGGC AAAAAGGGTC CTGATGGTCC TCCTGGAAAA 
ATTGGTTTCC CTGGACACGC GGGAAAGATC GGCGAGTCTG GGGAGGTGGG GCCAAAGGGA 
TTTCCGGGGA TTCAAGGGCC ATCCGGGGCT ACAGGAGACA AAGGAATCGC AGGGGAGCCA 
GGACCGTCAG GACCTCCAGG AACACTCGGG CCTCAAGGAA ATCCCGGACC AAAGGGCCCT 
GCAGGAAAAG TTGGTGATTC TGGACTTCCA GGAGAACCTG GAGAAAAGGG GTCAATCGGG 
CTTGCAGGGA ATGCTGGGGC TGCAGGACTT ATCGGAGCAA GGGGAGAACC AGGTTTAGAG 
GGAGAGGCTG GTCCCGCTGG TCCTGATGGC ACAAAGGGTG AAAAGGGTGA CATGGGCACA 
GAAGGTGAGC AGGGGGTCAG AGGTGACCCT GGAATTAAGG GGAAAGACGG GCCACCGGGC 
GACCCTGGAC TTACTGGAGT CAGAGGTCCT GAAGGAAAAT CTGGTAAAAG TGGCGAGAGA 
GGGAAACCAG GGCTTAAGGG TGCCAAAGGT AACATCGGTC ATCTGGGCGA GACGGGAAGT 
GTGGGAAAGA TCGGGCCAAT CGGAACCACT GGACCAAAGG GCTCTCGAGG AACTATCGGA 
CATGCGGGTG CTCCTGGGCG TATGGGCTTG CAGGGCGATC CAGGTATATC AGGATATGAG 
GGTCACAAAG GACCTCAAGG TCCAATTGGA CCCCCGGGAC CAAAGGGTGC AAAGGGTGAA 
CAGGGGGATG ATGGGAAGGT GGAGGGTCCG ACCGGTGCAC CGGGTCTCAG AGGTCCCGTT 
GGAAAGCGGG GAGACAGGGG TGAGCCAGGA GATCCAGGAT ATGTGGGTCA GCAAGGTGTT 
GATGGTTTAC GTGGAAAACC TGGAGCTCCT GGACTGCCTG GCGATCCTGG TCCACGTGGA 
ACACAAGGCC CTAAGGGATC CAAAGGAGAA CAGGGTCAGA AGGGCAAACA GGGTCAGCAG 
GGAGAGCGTG GCAGCAGAGG ATCACCGGGT GTTGTTGGGT TGCCAGGTCC TCGAGGTACT 
GTGGGACGAG AAGGACGAGA GGGTTTTCCT GGTACAGATG GACTTGCTGG AAAGGATGGA 
AGCAGAGGAA CACCTGGCGA TCAGGGAGAT GATGGAGAGT TCGGTTTGCC TGGTAAACCT 
GGTGCACCTG GAAAAGTTGG CGTCATCGGT TTACCAGGCC CACAGGGGTC ATTCGGTCCA 
AAGGGTGAGC GAGGTCTCCC TGGTCACCCC GGCCCTTCAG GAAAGAGAGG ATTTAAGGGA 
GGAATGGGTC TTCCTGGACC TCAGGGAGAC CGGGGATCTA AAGGCCAACC TGGTGACATC 
GGGGAACCCG GCTTTCCAGG AATGCTGGGA ATGTTTGGAC CAAAGGGGCC TCCTGGAGAC 
TTTGGACCAA AGGGGATTCA GGGACCCAAA GGACCCCAAG GCAACATGGG CAGAGGTGGT 
TTAGCTGGAC CTGTCGGTGT GATCGGCCCT ATTGGTAATC CAGGCTCAAG AGGAGATACA 
GGCAACAAAG GAGAGCTGGG TGTTCAGGGT CCTCGAGGTG CTCCTGGACC ACGTGGACCT 
CCTGGACTTC CGGGTCCTCC GGGAATCCCG CTGGCCATGA ATCAGGATTT TGGTCTCGGT 
GTAGTTCAGC CAGTTTTCCG AGAGACTCAT ACACAGAAAA TTGAGGGTCG GGGTCTACTG 
GACATGCCCA TGTTGGATCA GGCTCCAGAG ATCCTGCGAA CGCTTGATTA TCTGAGCTCT 
CTAGTGCACA GCCTGAAGAA CCCACTGGGG ACACGAGACC ATCCCGCTCG ACTCTGCAGA 
GACCTGCATG ACTGCCGGGA CACGCTTTAC GATGGCACTT ACTGGATCGA CCCAAACCTG 
GGCTGCTCCT CAGACTCTAT AGAAGTGATG TGTAATTTCA GCAGTGGCGG ACGCACGTGT 
CTCAGACCCA TCACCACGGC CAAGCTGGAG TTCAGTGTTG GACGGGTTCA GATGAACTTT 
CTGCACCTGC TGAGCGCCGG AGCGGAGCAA CGAATCACTA TCCACTGCTT GAACGTGACT 
ATCTGGAGCC ACGCCCCCAA CCAGCCTCCT TCCCAAAATG CAGTTCAGTT CCACTCATGG 
ATCGGAGAGG TGCTGGAGCC GGATGTGTTG GAGGACACCT GCTGGCAATT GAACGGGCGC 
TGGCAGCACG CCGACTTCCT GTTCCGGGTG TTGGACCCTG CCTTACTCCC TGTCGTACGC 
ATAAGCAATC TCCCAAAGGT GATGCCCTCA TCACGCTTTC ACCTTGAAGT CGGACCTGTG 
TGCTTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish.
Nature communications8(1)126(2017 07)
Wehner D,Tsarouchas TM,Michael A,Haase C,Weidinger G,Reimer MM,Becker T,Becker CG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Collagen duplicate genes of bone and cartilage participate during regeneration of zebrafish fin skeleton.
Gene expression patterns : GEP19(1-2)60-9(2015 Sep-Nov)
Duran I,Csukasi F,Taylor SP,Krakow D,Becerra J,Bombarely A,Mar?-Beffa M

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col27a1a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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