med13a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	med13a
Entrez Gene ID	100004024
Full Name	mediator complex subunit 13a
Synonyms	im:7158513,med13,si:dkeyp-53h3.2,thrap1
General protein information	Preferred Names mediator complex subunit 13a Names mediator of RNA polymerase II transcription subunit 13 mediator complex subunit 13
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281468.1	NP_001268397.1	mediator of RNA polymerase II transcription subunit 13

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	med13a	XP_005172748.1
Xenopus tropicalis (tropical clawed frog)	med13	XP_004911678.1
Homo sapiens (human)	MED13	NP_005112.2
Pan troglodytes (chimpanzee)	MED13	XP_001138050.1
Drosophila melanogaster (fruit fly)	skd	NP_001262134.1
Canis lupus familiaris (dog)	MED13	XP_005625048.1
Bos taurus (cattle)	MED13	XP_002695649.2
Mus musculus (house mouse)	Med13	NP_001074400.1
	MED13	XP_001110128.2
Rattus norvegicus (Norway rat)	Med13	NP_001100505.1
Gallus gallus (chicken)	MED13	XP_004946777.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptional Complexity and Distinct Expression Patterns of
Kondrychyn I, Robra L, Thirumalai V
G3 (Bethesda, Md.)7(8)2577-2593(2017 Aug)

Depletion of Med10 enhances Wnt and suppresses Nodal signaling during zebrafish embryogenesis.
Lin X, Rinaldo L, Fazly AF, Xu X
Developmental biology303(2)536-48(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following med13a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med13a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36503	NM_001281468.1 Latest version!	Danio rerio mediator complex subunit 13a (med13a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

med13a ( NM_001281468.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001268397.1 Danio rerio mediator complex subunit 13a (med13a), mRNA.

Price & Availability↑

CloneID	ODa36503
Clone ID Related Accession (Same CDS sequence)	NM_001281468.1
Accession Version	NM_001281468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-08
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 13
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX000486.13 and CR925779.11. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01026155.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGCTT GTTTCGTACC GAACGGGGCC AGCTTGGAGG ACTGCCATTC CAATCTATTC 
TATCTGGCTG ATTTGACAGG GATAAAATGG AAGAGGTACG TTTGGCAAGG TCCGATCTCG 
GCACCCATGC TGTTCCCGGT CACCGAGGAA GACCCCATCC TCTGCAGCTT CAGTCGCTGT 
CTAAAGTCGG ATGTGCTGAG CGTGTGGAGA CGGAGCCAGA GACAGGGGCG CCGGGAGCTT 
TGGCTCTTCT GGTGGGGCGA CGATCCCAAC TTTGCTGACC TTATCCACCA CGAACTGACA 
GCAGAGGATG ATGGCGTGTG GGAGAGCGGT CTGTCCTACG AATGTCGAAC TCTTCTCTTT 
AAGGCCATTC ACAATCTGTT GGAGAGATGT CTGATGAACC GCAGTTTTGT TCGCATTGGA 
AAATGGTTCG TCAAACCCTA TGAAAAGGAT GAGAAGCCTA TAAACAAGAG AGAACACCTG 
TCTTGCTCCT TCACGTTCTT CCTGCATGGA GACAGCAATG TGTGCACCAG CGTGGAGATC 
AACCAGCACC AGCCCGTGTA CCACCTGAGC GAGGATCACC TGACACTGGC CCAGCAGGCC 
TCCAGCCCAT TCCAAGTGAT TCTGAGTCCA TTTGGACTGA ACGGGACACT GACCGGCCAG 
TCCTTTAAGC TGTCTGACCC TCCGACACAG AAGCTCATTG AAGAATGGAA CCAGTTTTAT 
CCCATCAGCC CAACCTCCAA GGAGAGCGCA GAGGACAAAA TGGAGGACAT AGACTGGGAG 
GACGATTCCC TGGCAGCAGT GGAAGTGCTT GTTGGTGGTG TGCGCATGGT ATATCCAGCA 
TGCCTGGTGT TAGTCCCGCA GTCAGACATC CCCACTATAA CACAGACGGG CTCCTCGCAC 
TGCACCGCTG TCTATTCAAA TGGCCATCAA GTGCCTGCTT CAAACCGTGA CCCTGCCATC 
TCTTCAGTGA CTCTGACTCC TCCCACCTCA CCTGAGGAGG CTCAGACAGT TCACTCTCAT 
TCGGCACAGA AGTGGATGCG GTTGCCGGTG GCGATGGATG GATTCAGTGT TGACAACACC 
AGTCATCACG GTGGCAAGAT TCCACGAAGG ATGGCCAGTC AGGTGGTTGA AACTGTCTGG 
CAAGAGTGCA ACATCAACCG AGCTCAGAAC AAACGGAAAT TCTCTGCCAC GGCCTCCAAC 
GGAACCAGCG AGGAGGAGAC ACTAATAAAA GTGGGCGCCT GGGACTTCGT GGACTCCACA 
CAGAGATCAA ACTGCAACTG TTCTAGGCAC AAAACACTGA AGCAGAGAGC AAGCAGCACA 
CCTGGCCAGC CACCCACCGC CGGTCAGACG CAGCAGCCGC CCATCAAGCA CAAAGCTGGG 
GAGAAGCCTG AGAAGGGAGA CAAGCAGCAG AAACGGCCGC AGACGCCTTT CCACCACCGC 
AACTCAGTCT GCGAAGATGT TACAATGGAA ACTGACGCCT CTGCGGGCCA GCGCTTGGCT 
CTCAGAGGTC AAGAGAGTGG ACGATTTCCT GGGCTCAGGC CTACTGATGT GGGCACTGGC 
TCGAAACCTC CACAGCTACA CAGTGGGGCT GGATCTACCG TAGGGAGCAG TGGCCCAACA 
GAGATGGTCG GCTCTCCTCA ACCGCCACCG CTTAGCCCTC ACCCTTGCGA ACGTGGCGAG 
GAACCCTCAG ACACCATTAA GAACCCCTCC ACTCCACAAC ATTTTTATCC TCCCTCCACA 
GAACCCTGCC TTTTGCCCCA GAAGGGTCCA GATGACGCCC GTTTGGAACC CCTCACCCAA 
CCTTTCCCCC CTGCATACCC CGAGACCCTG GAGCCCACCC CGTATATCAG CGCAGCAGTC 
AATATGGATG ACGATGGAAG TCATGCCCCA TGGAGGTTCT TTAACTTGCC TCGTAGGAAA 
GATGCGGAGT TTCCTACGCC GACACTACCG GGAGACAAAC TCCGAGAAGA AGCGTCACTC 
GGAGCTGAAG GGATTATGTC TGTTACTGAG TCAATGGCAG CCTCCAAAAG ACTGCTTAAG 
GTCTCAGATG AGCGGATCCA GATGTACCTG CAGCGACAGA ACCAGCACCT GGCTGCAGCC 
ATCACAGACG GCGACCACGA GCCAGAGGTC GACCCCTATG CCTTTGTCGA CGGAGATGTC 
AAGTTCACGT TCTCGGATAA GAAAGACAAA GCTGGATTAG AGAGAGAACC AGGAAAGAAA 
CACAAGGGAG AAGATGGCAA TGCTGCATCT GATGATGCTC AGCGGGCTGC AGCCCATAAC 
CGGGCTGCTT CCACCAGCCT CATACATGAT TCAGACCTGG CAGTCTCCAT CAAAGACCTA 
GACAACCTCT TCAACTCAGA TGAGGACGAA CAAACGCCTG GAGCGCGGAG AGCTGTAAAT 
GGAGCAGATG AGAAGTTTGG CAGCAAGGAG ACAAAGCCAG CCACATTGGA CCCTGTCTCC 
TGTATAAGCA CAGCCGATCT TCACCAGATG TTCCCTACGC CTCCGTCTTT AGAGCAGCAC 
TTCATGGGTT ACTCCCCCAT GAACATGGGC AGTAAAGACG GCTGTATGGA GCCTGGCTCT 
GGCCTCAACG CTCTGGATGG CCCCACTGCT CTGGGTGGCC CATTTAAAAT TGAGGTAGAG 
GAGAGCTTCT GCAGCCCCAA ACCTTCTGAA ATCAAGGACT TCTCATTTGT GTACAAGCCT 
GAGTTGTGTC AGCCATTTGT GGGGTGTTCA ATGTTTGCCC CGCTGAAGTC ATTGCCCAGT 
CAGTGCCTGC CCCCTATCAA ACTGCCTGAT GAGTGTGTTT ACAGGCCCAG CTGGACAGTG 
GGAAAACTGG AGCTACTTAA TTCTGTACCT GCTATGACCA TTTTCAGCAA GGATGGTGAA 
AACAATGCCA TGGAGCAAGA TTATATCCAG ACGTACACCC CTCAAACCCA TACGCCATTC 
TTGTCTAACA GTGCCCCACC CAGCAACAGT GGCACGAGCA TCCTCCCGTC TCCTGCCACG 
CCACGTTTCT CCGCTCCCAC CCCACGTACC CCACGGACAC CTCGGACGCC CAGAGGCCCA 
GCCAGTGTGC AAGGTTCACT CAAATATGAG AACTCGGATC TGTACTCGCC GGCTTCAACG 
CCTTCAACCT GCAGGCCGCT CAACTCTGTA GAGCCTGCGA CGGTTCCCTC CATCCCTGAA 
GCCCACAGCC TATACGTCAA CCTCATCCTG TCGGAGTCTG TCATGAACCT GTTTAAGGAC 
TGCAACTTCG ACAGCTGCTG CATCTGCGTA TGCAACATGA ACATCAAAGG TGCCGATGTG 
GGAATCTACA TCAACGATCC AAACATGGAG GCCCAATACC CCTGCGGATG CGGCTTCAGT 
GCTGTAGTCA ATCGGCGTTA CGGTAATGGC TCTGGCCTTT TCCTGGAAGA CGAGCTGGAT 
ATCATCGGAC GGGGTTCGGA TGTGAGCCAG GAGGCGGAGA AGCGCTTTGA GGCTTTACGG 
TGCATTTCAC TAGAGAAGAC AGGGGGTGGA GGCAAGGATC GAGTCCCGGA CGAGCTTATC 
TCTCTCCTGC AGGATCAGTG CACTAATCCC TTCTCTCCGA TCCTGGCACT GGACCCTGAG 
GGACTGTCTT CCCAAGGGCC AGGGGGCACC ACTGTACCAC CATGCGTGCG TGTAGAGGAG 
AGGGACTTCC ACAATGACTG TTACCTGGCT CTAGAACACG GGCGGCAGTT CATGGACAAC 
ATGTCCGGTG GGAAAGTGGA CGAGGTGTTA GTGAAGAGCA CGTGTTTGCA TCATTGGGCC 
AAACGTAGCG CGGTGGACGT GAGTGCGCTG TGCTCTCAGG ACGTGTTGAG AGTTCTTCTC 
TCTCTCCAGC CTGTTCTTCA GGATGCTATT CAGAAGAAGA GGACTGTTCG CTCCTGGGGA 
GTTCAGGGGC CTCTCACATG GCAGCAGTTT CACAAAATGG CTGGAAGAGG TTCTTACGGC 
ACTGACGAGT CTCCGGAGCC TCTGCCCATT CCTACCTTCC TGGTTGGGTA CGAGTATGAC 
TTTGTGGTGC TATCTCCCTT TGGCCTGCCG TATTGGGAGA AGCTACTACT GGATCCCTTT 
GGCTCTCAGA GAGATGTAGG ATATCTGGTC CTCTGCCCGG ACAGCGATTC TCTCCTCACT 
GGAGCCAAGA GTTTCTTTAG AGAGTTGACA GCGGTCTATG AGTCTTGTAA GCTGGGTCAA 
CATCGACCAA TCTCCAAAGT GCATGCTGAT GGCATTGTTA GGGTGGGTGG AGCTGCTGCC 
AAGAAGCTCT CAGAACAGCC GGTCAATGAT TGGTTCCTGA AAGCAGCTAG TGGCAGCAGT 
GATGCATTTG CCAAACTCAA ACTGTTTGCC CAAGTCTGCA GGCATGACCT CGCTCCATAC 
CTCGCTACAC AGCCATTGGA CAGCTCTCTC CTTATTCAGC CAACCCCTGT CCCTTCTTCC 
ACCCAATCGT CATCTTCACA ATCCTCAGGC TCCGCCTCCT CCAACTCTCA AACCTCAATG 
GCCTCTGGAG GCTCCGCCCT CAACAACAAC AACAACAGTG CTGCCAGCAA CAGTGGCTCA 
GGCCTGCCAG GCAGCACCAA TAACACACCC TCCTCCTCCT CCTCAGGCCC ACAGGGCAGC 
AGCATTCAGT CTGCCAAGCC CAGCTCGTTC CCTCCCTTTG GAAGCATGGG AGTCCAAGGT 
AACAGCTCCC AGTCTGGGGC TCTGGGCCAG CAGGCTGTCA CCCAGAACTC GAGCCTCTCT 
GGAGACAATT CAGCCATCAA CAGCCAGACT CAGGGGCCAT CTGAACCACC AGAGAGCACT 
ATGGAGAGAG AAAAGGTTGG AGTTCCTACA GACGGGGATT CTCATGCCAT CACATACCCA 
CCTGCAATCA TGATCTATAT CGTGGACCCC TTCTCATATG AGGAGGCGGA GCCAGGGACA 
TCACAATCAA GCAAGTGGAC TCTGGGACTC CTTCGCTGCT ACCTGGAGAT GCTCCAGGCC 
CTTCCAACTC ATATCCGCAA CTCTGTCTTT GTACAGATCG TCCCCTGTCA GTATCTGCTC 
CAGCCTGTGA GGAGTGAAGA AAGGCACCTT TACGCCCAGC ACCTCAAGTC TCTGGCCTTC 
TCCGTATTCA CCCAGTGCAG AAGACCCCTC CCAACCTCCA CCAATGTGAA GTCATTGACG 
GGTTTCGGCC CAGGCCTCGC CCTTGACACC TCGCTCAGGA ACCCAGATAG ACCAGAGTGT 
CTGCGATTGT ACACGCCACC ATTCATCCTG GCCCCAACGA AAGACAAGCA AACGGAGCTG 
GGTGAGACTT TCGGAGAGGC GTCTCAGAAG TATAACGTCC TTTTTGTTGG CTACTGCCTG 
TCGCATGATC AAAGGTGGCT GTTGGCAACT TGCACGGACC TGTACGGAGA GCTGCTGGAA 
ACTTGTATCA TCAGCGTAGA TGTACCCAAC AGAGCAAGAA GGAAAAAGGG ATCTGCACGG 
AAGATGGGTC TGCAGAAGCT TTGGGAGTGG TGTTTGGGTT TGGTTCAAAT GACATCGTTG 
CCATGGAGAG TTGTGATTGG CCGGCTTGGA CGGATAGGGC ATGGAGAGTT GCGAGACTGG 
AGTATTTTAC TGAGCAGACG GAATCTGCAG TCGGTGTCTC GGAGGCTGAA GGAGAACTGT 
AAGCTGTGCG GGATCTCAGC ATCTGACACT CCCAGCATTC TTAGCGCCTG CCTGGTTGCC 
ATGGAACCCC AGGGCTCATT TGTCATCATG CCAGACTCAG TGTCAACAGG CTCGGTGTTT 
GGTCGCAGTA CAACTCTAAA CATGCAGACG TCTCAGCTGA GTACGCCGCA GGACACGTCT 
TGCACACACA TCCTGGTGTT CCCCACCTCT GCCTTTGTGC AAGTCAACAG CTCCAACTAC 
ACACATGAGA ACATTGACAT TGCCTTCAAT CCTGGCAATG ATGGATCGGA TCCAATGGGA 
GGCATCTTTG ACCTACTGGA GCAGGAGAAT GACCTGGTTG ATCCAGATAT CATAAACATC 
CTCCCTGCCT CACCCACCAC ATCACCAGTC CACTCACCCG GATCCCAGTA CCCGCATGGA 
GGAGACGGGA GCAAGGGTCA AAGTACAGAC CGCATGGAGT CTCACGAAGA AGCTCCCAAT 
ATCCTCCAGC AGCCATTAGC TCTGGGCTAT TTTGTGTCCA CAGCAAAAGC AGGTCCACTG 
CCTGACTGGT TCTGGTCCGC GTGTCCTCAG GCACAGAACC AGTGTCCCCT TTTCTTAAAG 
GCCTCTCTAC ACCTCCATGT GTCTTCAGTG CAATCGGATG AACTTTTGCA CAGCAAACAC 
TCTCACCCTC TGGACTCCAA TCAGACCTCT GATGTTCTCA GATTTGTGTT GGAGCAGTAC 
AATGCCCTTT CCTGGCTGAC CTGTGACCCT GCGACCCAGG ACCGCCGCTC TTGCCTGCCC 
ATCCATTTTG TGGTGCTCAA TCAAATGTAC AACTTCATCA TGACCATGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268397.1
CDS	1..6474
Translation	MSACFVPNGASLEDCHSNLFYLADLTGIKWKRYVWQGPISAPMLFPVTEEDPILCSFSRCLKSDVLSVWRRSQRQGRRELWLFWWGDDPNFADLIHHELTAEDDGVWESGLSYECRTLLFKAIHNLLERCLMNRSFVRIGKWFVKPYEKDEKPINKREHLSCSFTFFLHGDSNVCTSVEINQHQPVYHLSEDHLTLAQQASSPFQVILSPFGLNGTLTGQSFKLSDPPTQKLIEEWNQFYPISPTSKESAEDKMEDIDWEDDSLAAVEVLVGGVRMVYPACLVLVPQSDIPTITQTGSSHCTAVYSNGHQVPASNRDPAISSVTLTPPTSPEEAQTVHSHSAQKWMRLPVAMDGFSVDNTSHHGGKIPRRMASQVVETVWQECNINRAQNKRKFSATASNGTSEEETLIKVGAWDFVDSTQRSNCNCSRHKTLKQRASSTPGQPPTAGQTQQPPIKHKAGEKPEKGDKQQKRPQTPFHHRNSVCEDVTMETDASAGQRLALRGQESGRFPGLRPTDVGTGSKPPQLHSGAGSTVGSSGPTEMVGSPQPPPLSPHPCERGEEPSDTIKNPSTPQHFYPPSTEPCLLPQKGPDDARLEPLTQPFPPAYPETLEPTPYISAAVNMDDDGSHAPWRFFNLPRRKDAEFPTPTLPGDKLREEASLGAEGIMSVTESMAASKRLLKVSDERIQMYLQRQNQHLAAAITDGDHEPEVDPYAFVDGDVKFTFSDKKDKAGLEREPGKKHKGEDGNAASDDAQRAAAHNRAASTSLIHDSDLAVSIKDLDNLFNSDEDEQTPGARRAVNGADEKFGSKETKPATLDPVSCISTADLHQMFPTPPSLEQHFMGYSPMNMGSKDGCMEPGSGLNALDGPTALGGPFKIEVEESFCSPKPSEIKDFSFVYKPELCQPFVGCSMFAPLKSLPSQCLPPIKLPDECVYRPSWTVGKLELLNSVPAMTIFSKDGENNAMEQDYIQTYTPQTHTPFLSNSAPPSNSGTSILPSPATPRFSAPTPRTPRTPRTPRGPASVQGSLKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGIYINDPNMEAQYPCGCGFSAVVNRRYGNGSGLFLEDELDIIGRGSDVSQEAEKRFEALRCISLEKTGGGGKDRVPDELISLLQDQCTNPFSPILALDPEGLSSQGPGGTTVPPCVRVEERDFHNDCYLALEHGRQFMDNMSGGKVDEVLVKSTCLHHWAKRSAVDVSALCSQDVLRVLLSLQPVLQDAIQKKRTVRSWGVQGPLTWQQFHKMAGRGSYGTDESPEPLPIPTFLVGYEYDFVVLSPFGLPYWEKLLLDPFGSQRDVGYLVLCPDSDSLLTGAKSFFRELTAVYESCKLGQHRPISKVHADGIVRVGGAAAKKLSEQPVNDWFLKAASGSSDAFAKLKLFAQVCRHDLAPYLATQPLDSSLLIQPTPVPSSTQSSSSQSSGSASSNSQTSMASGGSALNNNNNSAASNSGSGLPGSTNNTPSSSSSGPQGSSIQSAKPSSFPPFGSMGVQGNSSQSGALGQQAVTQNSSLSGDNSAINSQTQGPSEPPESTMEREKVGVPTDGDSHAITYPPAIMIYIVDPFSYEEAEPGTSQSSKWTLGLLRCYLEMLQALPTHIRNSVFVQIVPCQYLLQPVRSEERHLYAQHLKSLAFSVFTQCRRPLPTSTNVKSLTGFGPGLALDTSLRNPDRPECLRLYTPPFILAPTKDKQTELGETFGEASQKYNVLFVGYCLSHDQRWLLATCTDLYGELLETCIISVDVPNRARRKKGSARKMGLQKLWEWCLGLVQMTSLPWRVVIGRLGRIGHGELRDWSILLSRRNLQSVSRRLKENCKLCGISASDTPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTSQLSTPQDTSCTHILVFPTSAFVQVNSSNYTHENIDIAFNPGNDGSDPMGGIFDLLEQENDLVDPDIINILPASPTTSPVHSPGSQYPHGGDGSKGQSTDRMESHEEAPNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQNQCPLFLKASLHLHVSSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPATQDRRSCLPIHFVVLNQMYNFIMTML

Target ORF information:

RefSeq Version	NM_001281468.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 13a (med13a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281468.1

ORF Insert Sequence:

ATGAGTGCTT GTTTCGTACC GAACGGGGCC AGCTTGGAGG ACTGCCATTC CAATCTATTC 
TATCTGGCTG ATTTGACAGG GATAAAATGG AAGAGGTACG TTTGGCAAGG TCCGATCTCG 
GCACCCATGC TGTTCCCGGT CACCGAGGAA GACCCCATCC TCTGCAGCTT CAGTCGCTGT 
CTAAAGTCGG ATGTGCTGAG CGTGTGGAGA CGGAGCCAGA GACAGGGGCG CCGGGAGCTT 
TGGCTCTTCT GGTGGGGCGA CGATCCCAAC TTTGCTGACC TTATCCACCA CGAACTGACA 
GCAGAGGATG ATGGCGTGTG GGAGAGCGGT CTGTCCTACG AATGTCGAAC TCTTCTCTTT 
AAGGCCATTC ACAATCTGTT GGAGAGATGT CTGATGAACC GCAGTTTTGT TCGCATTGGA 
AAATGGTTCG TCAAACCCTA TGAAAAGGAT GAGAAGCCTA TAAACAAGAG AGAACACCTG 
TCTTGCTCCT TCACGTTCTT CCTGCATGGA GACAGCAATG TGTGCACCAG CGTGGAGATC 
AACCAGCACC AGCCCGTGTA CCACCTGAGC GAGGATCACC TGACACTGGC CCAGCAGGCC 
TCCAGCCCAT TCCAAGTGAT TCTGAGTCCA TTTGGACTGA ACGGGACACT GACCGGCCAG 
TCCTTTAAGC TGTCTGACCC TCCGACACAG AAGCTCATTG AAGAATGGAA CCAGTTTTAT 
CCCATCAGCC CAACCTCCAA GGAGAGCGCA GAGGACAAAA TGGAGGACAT AGACTGGGAG 
GACGATTCCC TGGCAGCAGT GGAAGTGCTT GTTGGTGGTG TGCGCATGGT ATATCCAGCA 
TGCCTGGTGT TAGTCCCGCA GTCAGACATC CCCACTATAA CACAGACGGG CTCCTCGCAC 
TGCACCGCTG TCTATTCAAA TGGCCATCAA GTGCCTGCTT CAAACCGTGA CCCTGCCATC 
TCTTCAGTGA CTCTGACTCC TCCCACCTCA CCTGAGGAGG CTCAGACAGT TCACTCTCAT 
TCGGCACAGA AGTGGATGCG GTTGCCGGTG GCGATGGATG GATTCAGTGT TGACAACACC 
AGTCATCACG GTGGCAAGAT TCCACGAAGG ATGGCCAGTC AGGTGGTTGA AACTGTCTGG 
CAAGAGTGCA ACATCAACCG AGCTCAGAAC AAACGGAAAT TCTCTGCCAC GGCCTCCAAC 
GGAACCAGCG AGGAGGAGAC ACTAATAAAA GTGGGCGCCT GGGACTTCGT GGACTCCACA 
CAGAGATCAA ACTGCAACTG TTCTAGGCAC AAAACACTGA AGCAGAGAGC AAGCAGCACA 
CCTGGCCAGC CACCCACCGC CGGTCAGACG CAGCAGCCGC CCATCAAGCA CAAAGCTGGG 
GAGAAGCCTG AGAAGGGAGA CAAGCAGCAG AAACGGCCGC AGACGCCTTT CCACCACCGC 
AACTCAGTCT GCGAAGATGT TACAATGGAA ACTGACGCCT CTGCGGGCCA GCGCTTGGCT 
CTCAGAGGTC AAGAGAGTGG ACGATTTCCT GGGCTCAGGC CTACTGATGT GGGCACTGGC 
TCGAAACCTC CACAGCTACA CAGTGGGGCT GGATCTACCG TAGGGAGCAG TGGCCCAACA 
GAGATGGTCG GCTCTCCTCA ACCGCCACCG CTTAGCCCTC ACCCTTGCGA ACGTGGCGAG 
GAACCCTCAG ACACCATTAA GAACCCCTCC ACTCCACAAC ATTTTTATCC TCCCTCCACA 
GAACCCTGCC TTTTGCCCCA GAAGGGTCCA GATGACGCCC GTTTGGAACC CCTCACCCAA 
CCTTTCCCCC CTGCATACCC CGAGACCCTG GAGCCCACCC CGTATATCAG CGCAGCAGTC 
AATATGGATG ACGATGGAAG TCATGCCCCA TGGAGGTTCT TTAACTTGCC TCGTAGGAAA 
GATGCGGAGT TTCCTACGCC GACACTACCG GGAGACAAAC TCCGAGAAGA AGCGTCACTC 
GGAGCTGAAG GGATTATGTC TGTTACTGAG TCAATGGCAG CCTCCAAAAG ACTGCTTAAG 
GTCTCAGATG AGCGGATCCA GATGTACCTG CAGCGACAGA ACCAGCACCT GGCTGCAGCC 
ATCACAGACG GCGACCACGA GCCAGAGGTC GACCCCTATG CCTTTGTCGA CGGAGATGTC 
AAGTTCACGT TCTCGGATAA GAAAGACAAA GCTGGATTAG AGAGAGAACC AGGAAAGAAA 
CACAAGGGAG AAGATGGCAA TGCTGCATCT GATGATGCTC AGCGGGCTGC AGCCCATAAC 
CGGGCTGCTT CCACCAGCCT CATACATGAT TCAGACCTGG CAGTCTCCAT CAAAGACCTA 
GACAACCTCT TCAACTCAGA TGAGGACGAA CAAACGCCTG GAGCGCGGAG AGCTGTAAAT 
GGAGCAGATG AGAAGTTTGG CAGCAAGGAG ACAAAGCCAG CCACATTGGA CCCTGTCTCC 
TGTATAAGCA CAGCCGATCT TCACCAGATG TTCCCTACGC CTCCGTCTTT AGAGCAGCAC 
TTCATGGGTT ACTCCCCCAT GAACATGGGC AGTAAAGACG GCTGTATGGA GCCTGGCTCT 
GGCCTCAACG CTCTGGATGG CCCCACTGCT CTGGGTGGCC CATTTAAAAT TGAGGTAGAG 
GAGAGCTTCT GCAGCCCCAA ACCTTCTGAA ATCAAGGACT TCTCATTTGT GTACAAGCCT 
GAGTTGTGTC AGCCATTTGT GGGGTGTTCA ATGTTTGCCC CGCTGAAGTC ATTGCCCAGT 
CAGTGCCTGC CCCCTATCAA ACTGCCTGAT GAGTGTGTTT ACAGGCCCAG CTGGACAGTG 
GGAAAACTGG AGCTACTTAA TTCTGTACCT GCTATGACCA TTTTCAGCAA GGATGGTGAA 
AACAATGCCA TGGAGCAAGA TTATATCCAG ACGTACACCC CTCAAACCCA TACGCCATTC 
TTGTCTAACA GTGCCCCACC CAGCAACAGT GGCACGAGCA TCCTCCCGTC TCCTGCCACG 
CCACGTTTCT CCGCTCCCAC CCCACGTACC CCACGGACAC CTCGGACGCC CAGAGGCCCA 
GCCAGTGTGC AAGGTTCACT CAAATATGAG AACTCGGATC TGTACTCGCC GGCTTCAACG 
CCTTCAACCT GCAGGCCGCT CAACTCTGTA GAGCCTGCGA CGGTTCCCTC CATCCCTGAA 
GCCCACAGCC TATACGTCAA CCTCATCCTG TCGGAGTCTG TCATGAACCT GTTTAAGGAC 
TGCAACTTCG ACAGCTGCTG CATCTGCGTA TGCAACATGA ACATCAAAGG TGCCGATGTG 
GGAATCTACA TCAACGATCC AAACATGGAG GCCCAATACC CCTGCGGATG CGGCTTCAGT 
GCTGTAGTCA ATCGGCGTTA CGGTAATGGC TCTGGCCTTT TCCTGGAAGA CGAGCTGGAT 
ATCATCGGAC GGGGTTCGGA TGTGAGCCAG GAGGCGGAGA AGCGCTTTGA GGCTTTACGG 
TGCATTTCAC TAGAGAAGAC AGGGGGTGGA GGCAAGGATC GAGTCCCGGA CGAGCTTATC 
TCTCTCCTGC AGGATCAGTG CACTAATCCC TTCTCTCCGA TCCTGGCACT GGACCCTGAG 
GGACTGTCTT CCCAAGGGCC AGGGGGCACC ACTGTACCAC CATGCGTGCG TGTAGAGGAG 
AGGGACTTCC ACAATGACTG TTACCTGGCT CTAGAACACG GGCGGCAGTT CATGGACAAC 
ATGTCCGGTG GGAAAGTGGA CGAGGTGTTA GTGAAGAGCA CGTGTTTGCA TCATTGGGCC 
AAACGTAGCG CGGTGGACGT GAGTGCGCTG TGCTCTCAGG ACGTGTTGAG AGTTCTTCTC 
TCTCTCCAGC CTGTTCTTCA GGATGCTATT CAGAAGAAGA GGACTGTTCG CTCCTGGGGA 
GTTCAGGGGC CTCTCACATG GCAGCAGTTT CACAAAATGG CTGGAAGAGG TTCTTACGGC 
ACTGACGAGT CTCCGGAGCC TCTGCCCATT CCTACCTTCC TGGTTGGGTA CGAGTATGAC 
TTTGTGGTGC TATCTCCCTT TGGCCTGCCG TATTGGGAGA AGCTACTACT GGATCCCTTT 
GGCTCTCAGA GAGATGTAGG ATATCTGGTC CTCTGCCCGG ACAGCGATTC TCTCCTCACT 
GGAGCCAAGA GTTTCTTTAG AGAGTTGACA GCGGTCTATG AGTCTTGTAA GCTGGGTCAA 
CATCGACCAA TCTCCAAAGT GCATGCTGAT GGCATTGTTA GGGTGGGTGG AGCTGCTGCC 
AAGAAGCTCT CAGAACAGCC GGTCAATGAT TGGTTCCTGA AAGCAGCTAG TGGCAGCAGT 
GATGCATTTG CCAAACTCAA ACTGTTTGCC CAAGTCTGCA GGCATGACCT CGCTCCATAC 
CTCGCTACAC AGCCATTGGA CAGCTCTCTC CTTATTCAGC CAACCCCTGT CCCTTCTTCC 
ACCCAATCGT CATCTTCACA ATCCTCAGGC TCCGCCTCCT CCAACTCTCA AACCTCAATG 
GCCTCTGGAG GCTCCGCCCT CAACAACAAC AACAACAGTG CTGCCAGCAA CAGTGGCTCA 
GGCCTGCCAG GCAGCACCAA TAACACACCC TCCTCCTCCT CCTCAGGCCC ACAGGGCAGC 
AGCATTCAGT CTGCCAAGCC CAGCTCGTTC CCTCCCTTTG GAAGCATGGG AGTCCAAGGT 
AACAGCTCCC AGTCTGGGGC TCTGGGCCAG CAGGCTGTCA CCCAGAACTC GAGCCTCTCT 
GGAGACAATT CAGCCATCAA CAGCCAGACT CAGGGGCCAT CTGAACCACC AGAGAGCACT 
ATGGAGAGAG AAAAGGTTGG AGTTCCTACA GACGGGGATT CTCATGCCAT CACATACCCA 
CCTGCAATCA TGATCTATAT CGTGGACCCC TTCTCATATG AGGAGGCGGA GCCAGGGACA 
TCACAATCAA GCAAGTGGAC TCTGGGACTC CTTCGCTGCT ACCTGGAGAT GCTCCAGGCC 
CTTCCAACTC ATATCCGCAA CTCTGTCTTT GTACAGATCG TCCCCTGTCA GTATCTGCTC 
CAGCCTGTGA GGAGTGAAGA AAGGCACCTT TACGCCCAGC ACCTCAAGTC TCTGGCCTTC 
TCCGTATTCA CCCAGTGCAG AAGACCCCTC CCAACCTCCA CCAATGTGAA GTCATTGACG 
GGTTTCGGCC CAGGCCTCGC CCTTGACACC TCGCTCAGGA ACCCAGATAG ACCAGAGTGT 
CTGCGATTGT ACACGCCACC ATTCATCCTG GCCCCAACGA AAGACAAGCA AACGGAGCTG 
GGTGAGACTT TCGGAGAGGC GTCTCAGAAG TATAACGTCC TTTTTGTTGG CTACTGCCTG 
TCGCATGATC AAAGGTGGCT GTTGGCAACT TGCACGGACC TGTACGGAGA GCTGCTGGAA 
ACTTGTATCA TCAGCGTAGA TGTACCCAAC AGAGCAAGAA GGAAAAAGGG ATCTGCACGG 
AAGATGGGTC TGCAGAAGCT TTGGGAGTGG TGTTTGGGTT TGGTTCAAAT GACATCGTTG 
CCATGGAGAG TTGTGATTGG CCGGCTTGGA CGGATAGGGC ATGGAGAGTT GCGAGACTGG 
AGTATTTTAC TGAGCAGACG GAATCTGCAG TCGGTGTCTC GGAGGCTGAA GGAGAACTGT 
AAGCTGTGCG GGATCTCAGC ATCTGACACT CCCAGCATTC TTAGCGCCTG CCTGGTTGCC 
ATGGAACCCC AGGGCTCATT TGTCATCATG CCAGACTCAG TGTCAACAGG CTCGGTGTTT 
GGTCGCAGTA CAACTCTAAA CATGCAGACG TCTCAGCTGA GTACGCCGCA GGACACGTCT 
TGCACACACA TCCTGGTGTT CCCCACCTCT GCCTTTGTGC AAGTCAACAG CTCCAACTAC 
ACACATGAGA ACATTGACAT TGCCTTCAAT CCTGGCAATG ATGGATCGGA TCCAATGGGA 
GGCATCTTTG ACCTACTGGA GCAGGAGAAT GACCTGGTTG ATCCAGATAT CATAAACATC 
CTCCCTGCCT CACCCACCAC ATCACCAGTC CACTCACCCG GATCCCAGTA CCCGCATGGA 
GGAGACGGGA GCAAGGGTCA AAGTACAGAC CGCATGGAGT CTCACGAAGA AGCTCCCAAT 
ATCCTCCAGC AGCCATTAGC TCTGGGCTAT TTTGTGTCCA CAGCAAAAGC AGGTCCACTG 
CCTGACTGGT TCTGGTCCGC GTGTCCTCAG GCACAGAACC AGTGTCCCCT TTTCTTAAAG 
GCCTCTCTAC ACCTCCATGT GTCTTCAGTG CAATCGGATG AACTTTTGCA CAGCAAACAC 
TCTCACCCTC TGGACTCCAA TCAGACCTCT GATGTTCTCA GATTTGTGTT GGAGCAGTAC 
AATGCCCTTT CCTGGCTGAC CTGTGACCCT GCGACCCAGG ACCGCCGCTC TTGCCTGCCC 
ATCCATTTTG TGGTGCTCAA TCAAATGTAC AACTTCATCA TGACCATGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptional Complexity and Distinct Expression Patterns of
G3 (Bethesda, Md.)7(8)2577-2593(2017 Aug)
Kondrychyn I,Robra L,Thirumalai V

Depletion of Med10 enhances Wnt and suppresses Nodal signaling during zebrafish embryogenesis.
Developmental biology303(2)536-48(2007 Mar)
Lin X,Rinaldo L,Fazly AF,Xu X

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med13a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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