znf516 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	znf516
Entrez Gene ID	100003866
Full Name	zinc finger protein 516
Synonyms	si:ch211-135j15.1
General protein information	Preferred Names zinc finger protein 516 Names zinc finger protein 516
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005169990.3	XP_005170047.1	zinc finger protein 516 isoform X1
NM_001159669.1	NP_001153141.1	zinc finger protein 516
XM_005169989.3	XP_005170046.1	zinc finger protein 516 isoform X1
XM_005169991.3	XP_005170048.1	zinc finger protein 516 isoform X2
XM_005169990.4	XP_005170047.1	zinc finger protein 516 isoform X1
XM_005169989.4	XP_005170046.1	zinc finger protein 516 isoform X1
XM_005169991.4	XP_005170048.1	zinc finger protein 516 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZNF516	NP_055458.1
Bos taurus (cattle)	ZNF516	XP_002697699.2
Danio rerio (zebrafish)	LOC100003866	NP_001153141.1
Xenopus tropicalis (tropical clawed frog)	LOC100036701	NP_001090719.1
	ZNF516	XP_001095926.2
Canis lupus familiaris (dog)	ZNF516	XP_005615361.1
Rattus norvegicus (Norway rat)	Zfp516	NP_001178624.1
Pan troglodytes (chimpanzee)	ZNF516	XP_003315994.1
Gallus gallus (chicken)	ZNF516	XP_419095.4
Mus musculus (house mouse)	Zfp516	NP_001170935.1

Gene Ontology
Bibliography

Related articles in PubMed

Melanoma genome evolution across species.
Kansler ER, Verma A, Langdon EM, Simon-Vermot T, Yin A, Lee W, Attiyeh M, Elemento O, White RM
BMC genomics18(1)136(2017 Feb)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following znf516 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the znf516 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa24102	NM_001159669.1 Latest version!	Danio rerio zinc finger protein 516 (znf516), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa24102	XM_005169989.4 Latest version!	Danio rerio zinc finger protein 516 (znf516), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa24102	XM_005169990.4 Latest version!	Danio rerio zinc finger protein 516 (znf516), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa24103	XM_005169991.4 Latest version!	Danio rerio zinc finger protein 516 (znf516), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa24102	XM_005169990.3	Danio rerio zinc finger protein 516 (znf516), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa24102	XM_005169989.3	Danio rerio zinc finger protein 516 (znf516), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa24103	XM_005169991.3	Danio rerio zinc finger protein 516 (znf516), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

znf516 ( NM_001159669.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001153141.1 Danio rerio zinc finger protein 516 (znf516), mRNA.

Price & Availability↑

CloneID	ODa24102
Clone ID Related Accession (Same CDS sequence)	XM_005169990.3 , NM_001159669.1 , XM_005169989.3 , XM_005169990.4 , XM_005169989.4
Accession Version	NM_001159669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	zinc finger protein 516
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CU467863.10 and CR388011.14. On Apr 29, 2009 this sequence version replaced XM_001343278.2. ##Evidence-Data-START## Transcript exon combination :: GDQH01019921.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476751, SAMEA4476768 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGGTGAG 
CGACCTTTCC AGTGCCGATA CTGTCCTTAC AGCGCCTCGC AGAAAGGTAA CCTGAAGACT 
CATGTTCAGA CCGTGCACCG TCTAGCCTTC GACAACGCGC AGTACCCGGA CAGGAGACTC 
CGCCAAGTCC CTACCAATGA ACCCATGGAC CCCCCTGGAT CCCTTCAAGC ACCTCCTCAT 
GACCAGCTCG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153141.1
CDS	1..3255
Translation	MEVERPEVSEQEPFSKGGHDNPDVKKSRGHTCELCGRSFPFLSSLSQHMRKHTGEKPYKCPHCEHRSAQKGSLKAHIRSHKLDGLSQSGGGEEEDMDEEGEKEGGVPEDQGSCSSPTESTSACNKVVSGEDATKMRKKSGKKERIPGESGKQSVQCSLCRKRLSGQAELEKHMQELHKVLRCELCPYETQQEDQLQAHVEKDHPSDEESITKEVSTSEETDGILGKGEFPCEQCDQVFTQAWCLKTHMKKHQNSMDHGCRICGRRFREPWFLRSHMKTHNTKVKPRSNSYLPATVNEVAQDESNLVNEVCLYELCAKCGNFFHDRKSLQLHEQVHKNIEQPLKNKNCNENNFPSVTKTSFLQCLNLKPAGNIENPSEGTIGKRIPELDPVSSYQAWQLATRGKLVEVTERSQGWEERLADADVAYDREKGEYVLLRQDKRKKSLDSTVSIPTKKRRGAVTQGCTPDQHSNGEKNSHVSTGERSPENLSDSEYRPTSRPSRKNSQKTSECLECGKGFRTQQQMVIHMLIRHGGLGETIGGNGLFRKTTSSPSKTGESAGHFRDQRRAGFLGDTDKKPYTCEHCDFCTSEPSAIAAHIQTHQSAVRDWGQRSDALTSSLSQSQTHQTNHTGFPRLRNALLQQPYWPYTTSTHLERAALSDAALKTEEEKSKGLEESSSTDKAESNLLNLSVDVDNKKEVSSILLNNLVRHQCPYCSYATHYPEVLWIHQRIAHKIDSTTLVPKWAPKNGLKGPKTLLDFKRRTGPPPFLEGKDCPSLPQMRSYRTSPPDCSPGGTKKSKPLTSTVESSSSQNKSWHTATAPGASSHSSKHKTSKSRTDELAGSKHKTDIHQNNSSRPMASPQKTGNPKTGSRVVESSLLPQEGLHFMLSSKHNLSDQRSSKAHAPQTPSKSDSQAQDTPSVAGYDPWNRLGLSGPSPHSQSKRQSTADGAEGVTDILSFLKNCNPHDLAALYHHWGYSSAMTEQAGLARSGSQQGEYVCPVCRKSFNQPSHYRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQTVHRLAFDNAQYPDRRLRQVPTNEPMDPPGSLQAPPHDQLE

Target ORF information:

RefSeq Version	NM_001159669.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zinc finger protein 516 (znf516), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159669.1

ORF Insert Sequence:

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGGTGAG 
CGACCTTTCC AGTGCCGATA CTGTCCTTAC AGCGCCTCGC AGAAAGGTAA CCTGAAGACT 
CATGTTCAGA CCGTGCACCG TCTAGCCTTC GACAACGCGC AGTACCCGGA CAGGAGACTC 
CGCCAAGTCC CTACCAATGA ACCCATGGAC CCCCCTGGAT CCCTTCAAGC ACCTCCTCAT 
GACCAGCTCG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_005169990.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170047.1 Danio rerio zinc finger protein 516 (znf516), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24102
Clone ID Related Accession (Same CDS sequence)	XM_005169990.3 , NM_001159669.1 , XM_005169989.3 , XM_005169990.4 , XM_005169989.4
Accession Version	XM_005169990.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	zinc finger protein 516 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169990.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGGTGAG 
CGACCTTTCC AGTGCCGATA CTGTCCTTAC AGCGCCTCGC AGAAAGGTAA CCTGAAGACT 
CATGTTCAGA CCGTGCACCG TCTAGCCTTC GACAACGCGC AGTACCCGGA CAGGAGACTC 
CGCCAAGTCC CTACCAATGA ACCCATGGAC CCCCCTGGAT CCCTTCAAGC ACCTCCTCAT 
GACCAGCTCG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170047.1
CDS	1037..4291
Translation	MEVERPEVSEQEPFSKGGHDNPDVKKSRGHTCELCGRSFPFLSSLSQHMRKHTGEKPYKCPHCEHRSAQKGSLKAHIRSHKLDGLSQSGGGEEEDMDEEGEKEGGVPEDQGSCSSPTESTSACNKVVSGEDATKMRKKSGKKERIPGESGKQSVQCSLCRKRLSGQAELEKHMQELHKVLRCELCPYETQQEDQLQAHVEKDHPSDEESITKEVSTSEETDGILGKGEFPCEQCDQVFTQAWCLKTHMKKHQNSMDHGCRICGRRFREPWFLRSHMKTHNTKVKPRSNSYLPATVNEVAQDESNLVNEVCLYELCAKCGNFFHDRKSLQLHEQVHKNIEQPLKNKNCNENNFPSVTKTSFLQCLNLKPAGNIENPSEGTIGKRIPELDPVSSYQAWQLATRGKLVEVTERSQGWEERLADADVAYDREKGEYVLLRQDKRKKSLDSTVSIPTKKRRGAVTQGCTPDQHSNGEKNSHVSTGERSPENLSDSEYRPTSRPSRKNSQKTSECLECGKGFRTQQQMVIHMLIRHGGLGETIGGNGLFRKTTSSPSKTGESAGHFRDQRRAGFLGDTDKKPYTCEHCDFCTSEPSAIAAHIQTHQSAVRDWGQRSDALTSSLSQSQTHQTNHTGFPRLRNALLQQPYWPYTTSTHLERAALSDAALKTEEEKSKGLEESSSTDKAESNLLNLSVDVDNKKEVSSILLNNLVRHQCPYCSYATHYPEVLWIHQRIAHKIDSTTLVPKWAPKNGLKGPKTLLDFKRRTGPPPFLEGKDCPSLPQMRSYRTSPPDCSPGGTKKSKPLTSTVESSSSQNKSWHTATAPGASSHSSKHKTSKSRTDELAGSKHKTDIHQNNSSRPMASPQKTGNPKTGSRVVESSLLPQEGLHFMLSSKHNLSDQRSSKAHAPQTPSKSDSQAQDTPSVAGYDPWNRLGLSGPSPHSQSKRQSTADGAEGVTDILSFLKNCNPHDLAALYHHWGYSSAMTEQAGLARSGSQQGEYVCPVCRKSFNQPSHYRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQTVHRLAFDNAQYPDRRLRQVPTNEPMDPPGSLQAPPHDQLE

Target ORF information:

RefSeq Version	XM_005169990.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zinc finger protein 516 (znf516), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169990.3

ORF Insert Sequence:

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGGTGAG 
CGACCTTTCC AGTGCCGATA CTGTCCTTAC AGCGCCTCGC AGAAAGGTAA CCTGAAGACT 
CATGTTCAGA CCGTGCACCG TCTAGCCTTC GACAACGCGC AGTACCCGGA CAGGAGACTC 
CGCCAAGTCC CTACCAATGA ACCCATGGAC CCCCCTGGAT CCCTTCAAGC ACCTCCTCAT 
GACCAGCTCG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_005169989.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170046.1 Danio rerio zinc finger protein 516 (znf516), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24102
Clone ID Related Accession (Same CDS sequence)	XM_005169990.3 , NM_001159669.1 , XM_005169989.3 , XM_005169990.4 , XM_005169989.4
Accession Version	XM_005169989.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	zinc finger protein 516 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169989.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGGTGAG 
CGACCTTTCC AGTGCCGATA CTGTCCTTAC AGCGCCTCGC AGAAAGGTAA CCTGAAGACT 
CATGTTCAGA CCGTGCACCG TCTAGCCTTC GACAACGCGC AGTACCCGGA CAGGAGACTC 
CGCCAAGTCC CTACCAATGA ACCCATGGAC CCCCCTGGAT CCCTTCAAGC ACCTCCTCAT 
GACCAGCTCG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170046.1
CDS	518..3772
Translation	MEVERPEVSEQEPFSKGGHDNPDVKKSRGHTCELCGRSFPFLSSLSQHMRKHTGEKPYKCPHCEHRSAQKGSLKAHIRSHKLDGLSQSGGGEEEDMDEEGEKEGGVPEDQGSCSSPTESTSACNKVVSGEDATKMRKKSGKKERIPGESGKQSVQCSLCRKRLSGQAELEKHMQELHKVLRCELCPYETQQEDQLQAHVEKDHPSDEESITKEVSTSEETDGILGKGEFPCEQCDQVFTQAWCLKTHMKKHQNSMDHGCRICGRRFREPWFLRSHMKTHNTKVKPRSNSYLPATVNEVAQDESNLVNEVCLYELCAKCGNFFHDRKSLQLHEQVHKNIEQPLKNKNCNENNFPSVTKTSFLQCLNLKPAGNIENPSEGTIGKRIPELDPVSSYQAWQLATRGKLVEVTERSQGWEERLADADVAYDREKGEYVLLRQDKRKKSLDSTVSIPTKKRRGAVTQGCTPDQHSNGEKNSHVSTGERSPENLSDSEYRPTSRPSRKNSQKTSECLECGKGFRTQQQMVIHMLIRHGGLGETIGGNGLFRKTTSSPSKTGESAGHFRDQRRAGFLGDTDKKPYTCEHCDFCTSEPSAIAAHIQTHQSAVRDWGQRSDALTSSLSQSQTHQTNHTGFPRLRNALLQQPYWPYTTSTHLERAALSDAALKTEEEKSKGLEESSSTDKAESNLLNLSVDVDNKKEVSSILLNNLVRHQCPYCSYATHYPEVLWIHQRIAHKIDSTTLVPKWAPKNGLKGPKTLLDFKRRTGPPPFLEGKDCPSLPQMRSYRTSPPDCSPGGTKKSKPLTSTVESSSSQNKSWHTATAPGASSHSSKHKTSKSRTDELAGSKHKTDIHQNNSSRPMASPQKTGNPKTGSRVVESSLLPQEGLHFMLSSKHNLSDQRSSKAHAPQTPSKSDSQAQDTPSVAGYDPWNRLGLSGPSPHSQSKRQSTADGAEGVTDILSFLKNCNPHDLAALYHHWGYSSAMTEQAGLARSGSQQGEYVCPVCRKSFNQPSHYRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQTVHRLAFDNAQYPDRRLRQVPTNEPMDPPGSLQAPPHDQLE

Target ORF information:

RefSeq Version	XM_005169989.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zinc finger protein 516 (znf516), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169989.3

ORF Insert Sequence:

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGGTGAG 
CGACCTTTCC AGTGCCGATA CTGTCCTTAC AGCGCCTCGC AGAAAGGTAA CCTGAAGACT 
CATGTTCAGA CCGTGCACCG TCTAGCCTTC GACAACGCGC AGTACCCGGA CAGGAGACTC 
CGCCAAGTCC CTACCAATGA ACCCATGGAC CCCCCTGGAT CCCTTCAAGC ACCTCCTCAT 
GACCAGCTCG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_005169990.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170047.1 Danio rerio zinc finger protein 516 (znf516), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24102
Clone ID Related Accession (Same CDS sequence)	XM_005169990.3 , NM_001159669.1 , XM_005169989.3 , XM_005169990.4 , XM_005169989.4
Accession Version	XM_005169990.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein 516 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169990.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGGTGAG 
CGACCTTTCC AGTGCCGATA CTGTCCTTAC AGCGCCTCGC AGAAAGGTAA CCTGAAGACT 
CATGTTCAGA CCGTGCACCG TCTAGCCTTC GACAACGCGC AGTACCCGGA CAGGAGACTC 
CGCCAAGTCC CTACCAATGA ACCCATGGAC CCCCCTGGAT CCCTTCAAGC ACCTCCTCAT 
GACCAGCTCG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170047.1
CDS	1033..4287
Translation	MEVERPEVSEQEPFSKGGHDNPDVKKSRGHTCELCGRSFPFLSSLSQHMRKHTGEKPYKCPHCEHRSAQKGSLKAHIRSHKLDGLSQSGGGEEEDMDEEGEKEGGVPEDQGSCSSPTESTSACNKVVSGEDATKMRKKSGKKERIPGESGKQSVQCSLCRKRLSGQAELEKHMQELHKVLRCELCPYETQQEDQLQAHVEKDHPSDEESITKEVSTSEETDGILGKGEFPCEQCDQVFTQAWCLKTHMKKHQNSMDHGCRICGRRFREPWFLRSHMKTHNTKVKPRSNSYLPATVNEVAQDESNLVNEVCLYELCAKCGNFFHDRKSLQLHEQVHKNIEQPLKNKNCNENNFPSVTKTSFLQCLNLKPAGNIENPSEGTIGKRIPELDPVSSYQAWQLATRGKLVEVTERSQGWEERLADADVAYDREKGEYVLLRQDKRKKSLDSTVSIPTKKRRGAVTQGCTPDQHSNGEKNSHVSTGERSPENLSDSEYRPTSRPSRKNSQKTSECLECGKGFRTQQQMVIHMLIRHGGLGETIGGNGLFRKTTSSPSKTGESAGHFRDQRRAGFLGDTDKKPYTCEHCDFCTSEPSAIAAHIQTHQSAVRDWGQRSDALTSSLSQSQTHQTNHTGFPRLRNALLQQPYWPYTTSTHLERAALSDAALKTEEEKSKGLEESSSTDKAESNLLNLSVDVDNKKEVSSILLNNLVRHQCPYCSYATHYPEVLWIHQRIAHKIDSTTLVPKWAPKNGLKGPKTLLDFKRRTGPPPFLEGKDCPSLPQMRSYRTSPPDCSPGGTKKSKPLTSTVESSSSQNKSWHTATAPGASSHSSKHKTSKSRTDELAGSKHKTDIHQNNSSRPMASPQKTGNPKTGSRVVESSLLPQEGLHFMLSSKHNLSDQRSSKAHAPQTPSKSDSQAQDTPSVAGYDPWNRLGLSGPSPHSQSKRQSTADGAEGVTDILSFLKNCNPHDLAALYHHWGYSSAMTEQAGLARSGSQQGEYVCPVCRKSFNQPSHYRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQTVHRLAFDNAQYPDRRLRQVPTNEPMDPPGSLQAPPHDQLE

Target ORF information:

RefSeq Version	XM_005169990.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zinc finger protein 516 (znf516), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169990.4

ORF Insert Sequence:

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGGTGAG 
CGACCTTTCC AGTGCCGATA CTGTCCTTAC AGCGCCTCGC AGAAAGGTAA CCTGAAGACT 
CATGTTCAGA CCGTGCACCG TCTAGCCTTC GACAACGCGC AGTACCCGGA CAGGAGACTC 
CGCCAAGTCC CTACCAATGA ACCCATGGAC CCCCCTGGAT CCCTTCAAGC ACCTCCTCAT 
GACCAGCTCG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_005169989.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170046.1 Danio rerio zinc finger protein 516 (znf516), transcript variant X1, mRNA.

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CloneID	ODa24102
Clone ID Related Accession (Same CDS sequence)	XM_005169990.3 , NM_001159669.1 , XM_005169989.3 , XM_005169990.4 , XM_005169989.4
Accession Version	XM_005169989.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein 516 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169989.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGGTGAG 
CGACCTTTCC AGTGCCGATA CTGTCCTTAC AGCGCCTCGC AGAAAGGTAA CCTGAAGACT 
CATGTTCAGA CCGTGCACCG TCTAGCCTTC GACAACGCGC AGTACCCGGA CAGGAGACTC 
CGCCAAGTCC CTACCAATGA ACCCATGGAC CCCCCTGGAT CCCTTCAAGC ACCTCCTCAT 
GACCAGCTCG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170046.1
CDS	583..3837
Translation	MEVERPEVSEQEPFSKGGHDNPDVKKSRGHTCELCGRSFPFLSSLSQHMRKHTGEKPYKCPHCEHRSAQKGSLKAHIRSHKLDGLSQSGGGEEEDMDEEGEKEGGVPEDQGSCSSPTESTSACNKVVSGEDATKMRKKSGKKERIPGESGKQSVQCSLCRKRLSGQAELEKHMQELHKVLRCELCPYETQQEDQLQAHVEKDHPSDEESITKEVSTSEETDGILGKGEFPCEQCDQVFTQAWCLKTHMKKHQNSMDHGCRICGRRFREPWFLRSHMKTHNTKVKPRSNSYLPATVNEVAQDESNLVNEVCLYELCAKCGNFFHDRKSLQLHEQVHKNIEQPLKNKNCNENNFPSVTKTSFLQCLNLKPAGNIENPSEGTIGKRIPELDPVSSYQAWQLATRGKLVEVTERSQGWEERLADADVAYDREKGEYVLLRQDKRKKSLDSTVSIPTKKRRGAVTQGCTPDQHSNGEKNSHVSTGERSPENLSDSEYRPTSRPSRKNSQKTSECLECGKGFRTQQQMVIHMLIRHGGLGETIGGNGLFRKTTSSPSKTGESAGHFRDQRRAGFLGDTDKKPYTCEHCDFCTSEPSAIAAHIQTHQSAVRDWGQRSDALTSSLSQSQTHQTNHTGFPRLRNALLQQPYWPYTTSTHLERAALSDAALKTEEEKSKGLEESSSTDKAESNLLNLSVDVDNKKEVSSILLNNLVRHQCPYCSYATHYPEVLWIHQRIAHKIDSTTLVPKWAPKNGLKGPKTLLDFKRRTGPPPFLEGKDCPSLPQMRSYRTSPPDCSPGGTKKSKPLTSTVESSSSQNKSWHTATAPGASSHSSKHKTSKSRTDELAGSKHKTDIHQNNSSRPMASPQKTGNPKTGSRVVESSLLPQEGLHFMLSSKHNLSDQRSSKAHAPQTPSKSDSQAQDTPSVAGYDPWNRLGLSGPSPHSQSKRQSTADGAEGVTDILSFLKNCNPHDLAALYHHWGYSSAMTEQAGLARSGSQQGEYVCPVCRKSFNQPSHYRTHMRSHTGERPFQCRYCPYSASQKGNLKTHVQTVHRLAFDNAQYPDRRLRQVPTNEPMDPPGSLQAPPHDQLE

Target ORF information:

RefSeq Version	XM_005169989.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zinc finger protein 516 (znf516), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169989.4

ORF Insert Sequence:

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGGTGAG 
CGACCTTTCC AGTGCCGATA CTGTCCTTAC AGCGCCTCGC AGAAAGGTAA CCTGAAGACT 
CATGTTCAGA CCGTGCACCG TCTAGCCTTC GACAACGCGC AGTACCCGGA CAGGAGACTC 
CGCCAAGTCC CTACCAATGA ACCCATGGAC CCCCCTGGAT CCCTTCAAGC ACCTCCTCAT 
GACCAGCTCG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_005169991.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170048.1 Danio rerio zinc finger protein 516 (znf516), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa24103
Clone ID Related Accession (Same CDS sequence)	XM_005169991.3 , XM_005169991.4
Accession Version	XM_005169991.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	zinc finger protein 516 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169991.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGTGATG 
TTTGAGTCCA ATGGACTCAT GGGAACTGGA GTCCATCCTC TACAGAAGCC CCCAAACAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170048.1
CDS	520..3642
Translation	MEVERPEVSEQEPFSKGGHDNPDVKKSRGHTCELCGRSFPFLSSLSQHMRKHTGEKPYKCPHCEHRSAQKGSLKAHIRSHKLDGLSQSGGGEEEDMDEEGEKEGGVPEDQGSCSSPTESTSACNKVVSGEDATKMRKKSGKKERIPGESGKQSVQCSLCRKRLSGQAELEKHMQELHKVLRCELCPYETQQEDQLQAHVEKDHPSDEESITKEVSTSEETDGILGKGEFPCEQCDQVFTQAWCLKTHMKKHQNSMDHGCRICGRRFREPWFLRSHMKTHNTKVKPRSNSYLPATVNEVAQDESNLVNEVCLYELCAKCGNFFHDRKSLQLHEQVHKNIEQPLKNKNCNENNFPSVTKTSFLQCLNLKPAGNIENPSEGTIGKRIPELDPVSSYQAWQLATRGKLVEVTERSQGWEERLADADVAYDREKGEYVLLRQDKRKKSLDSTVSIPTKKRRGAVTQGCTPDQHSNGEKNSHVSTGERSPENLSDSEYRPTSRPSRKNSQKTSECLECGKGFRTQQQMVIHMLIRHGGLGETIGGNGLFRKTTSSPSKTGESAGHFRDQRRAGFLGDTDKKPYTCEHCDFCTSEPSAIAAHIQTHQSAVRDWGQRSDALTSSLSQSQTHQTNHTGFPRLRNALLQQPYWPYTTSTHLERAALSDAALKTEEEKSKGLEESSSTDKAESNLLNLSVDVDNKKEVSSILLNNLVRHQCPYCSYATHYPEVLWIHQRIAHKIDSTTLVPKWAPKNGLKGPKTLLDFKRRTGPPPFLEGKDCPSLPQMRSYRTSPPDCSPGGTKKSKPLTSTVESSSSQNKSWHTATAPGASSHSSKHKTSKSRTDELAGSKHKTDIHQNNSSRPMASPQKTGNPKTGSRVVESSLLPQEGLHFMLSSKHNLSDQRSSKAHAPQTPSKSDSQAQDTPSVAGYDPWNRLGLSGPSPHSQSKRQSTADGAEGVTDILSFLKNCNPHDLAALYHHWGYSSAMTEQAGLARSGSQQGEYVCPVCRKSFNQPSHYRTHMRSHTVMFESNGLMGTGVHPLQKPPNK

Target ORF information:

RefSeq Version	XM_005169991.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zinc finger protein 516 (znf516), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169991.3

ORF Insert Sequence:

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGTGATG 
TTTGAGTCCA ATGGACTCAT GGGAACTGGA GTCCATCCTC TACAGAAGCC CCCAAACAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf516 ( XM_005169991.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170048.1 Danio rerio zinc finger protein 516 (znf516), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa24103
Clone ID Related Accession (Same CDS sequence)	XM_005169991.3 , XM_005169991.4
Accession Version	XM_005169991.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein 516 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169991.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGTGATG 
TTTGAGTCCA ATGGACTCAT GGGAACTGGA GTCCATCCTC TACAGAAGCC CCCAAACAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170048.1
CDS	588..3710
Translation	MEVERPEVSEQEPFSKGGHDNPDVKKSRGHTCELCGRSFPFLSSLSQHMRKHTGEKPYKCPHCEHRSAQKGSLKAHIRSHKLDGLSQSGGGEEEDMDEEGEKEGGVPEDQGSCSSPTESTSACNKVVSGEDATKMRKKSGKKERIPGESGKQSVQCSLCRKRLSGQAELEKHMQELHKVLRCELCPYETQQEDQLQAHVEKDHPSDEESITKEVSTSEETDGILGKGEFPCEQCDQVFTQAWCLKTHMKKHQNSMDHGCRICGRRFREPWFLRSHMKTHNTKVKPRSNSYLPATVNEVAQDESNLVNEVCLYELCAKCGNFFHDRKSLQLHEQVHKNIEQPLKNKNCNENNFPSVTKTSFLQCLNLKPAGNIENPSEGTIGKRIPELDPVSSYQAWQLATRGKLVEVTERSQGWEERLADADVAYDREKGEYVLLRQDKRKKSLDSTVSIPTKKRRGAVTQGCTPDQHSNGEKNSHVSTGERSPENLSDSEYRPTSRPSRKNSQKTSECLECGKGFRTQQQMVIHMLIRHGGLGETIGGNGLFRKTTSSPSKTGESAGHFRDQRRAGFLGDTDKKPYTCEHCDFCTSEPSAIAAHIQTHQSAVRDWGQRSDALTSSLSQSQTHQTNHTGFPRLRNALLQQPYWPYTTSTHLERAALSDAALKTEEEKSKGLEESSSTDKAESNLLNLSVDVDNKKEVSSILLNNLVRHQCPYCSYATHYPEVLWIHQRIAHKIDSTTLVPKWAPKNGLKGPKTLLDFKRRTGPPPFLEGKDCPSLPQMRSYRTSPPDCSPGGTKKSKPLTSTVESSSSQNKSWHTATAPGASSHSSKHKTSKSRTDELAGSKHKTDIHQNNSSRPMASPQKTGNPKTGSRVVESSLLPQEGLHFMLSSKHNLSDQRSSKAHAPQTPSKSDSQAQDTPSVAGYDPWNRLGLSGPSPHSQSKRQSTADGAEGVTDILSFLKNCNPHDLAALYHHWGYSSAMTEQAGLARSGSQQGEYVCPVCRKSFNQPSHYRTHMRSHTVMFESNGLMGTGVHPLQKPPNK

Target ORF information:

RefSeq Version	XM_005169991.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zinc finger protein 516 (znf516), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169991.4

ORF Insert Sequence:

ATGGAGGTGG AAAGGCCAGA AGTCTCAGAA CAAGAGCCCT TCTCCAAGGG TGGCCATGAC 
AACCCTGATG TCAAGAAGAG CCGGGGACAC ACTTGTGAAC TCTGTGGAAG AAGTTTTCCT 
TTCCTCAGCT CATTGTCCCA GCACATGAGA AAGCACACCG GGGAGAAACC CTACAAATGT 
CCTCATTGTG AGCACCGCTC AGCCCAAAAG GGCAGCTTAA AGGCCCACAT ACGCAGCCAC 
AAACTGGATG GTCTTAGCCA GAGTGGCGGG GGCGAGGAGG AAGATATGGA CGAGGAAGGG 
GAAAAGGAAG GAGGGGTGCC AGAAGATCAA GGTAGTTGCT CTAGTCCAAC TGAGAGTACC 
TCGGCTTGCA ACAAGGTTGT GAGTGGTGAG GATGCAACCA AAATGAGGAA GAAAAGTGGA 
AAGAAGGAAA GAATCCCTGG TGAGAGTGGT AAACAGTCAG TGCAGTGTTC TTTGTGCAGG 
AAAAGATTGT CCGGTCAGGC AGAGCTTGAA AAGCATATGC AGGAGCTTCA CAAGGTCTTG 
CGATGTGAAC TGTGCCCTTA TGAGACCCAG CAGGAGGACC AACTGCAGGC TCACGTTGAG 
AAGGATCATC CAAGTGATGA GGAATCTATC ACCAAAGAAG TGTCTACCAG CGAAGAAACT 
GATGGTATAC TTGGTAAAGG CGAGTTCCCA TGTGAGCAAT GTGACCAGGT TTTCACCCAA 
GCCTGGTGTC TAAAAACTCA CATGAAGAAA CATCAGAACA GTATGGATCA TGGGTGCCGG 
ATCTGTGGAC GTCGTTTTCG TGAGCCCTGG TTCCTACGTA GTCACATGAA GACCCACAAC 
ACCAAGGTAA AACCAAGGAG TAACTCTTAC CTTCCGGCCA CTGTCAACGA GGTGGCGCAG 
GACGAGTCCA ACTTGGTGAA TGAAGTGTGT CTGTATGAAC TCTGTGCCAA GTGTGGTAAC 
TTCTTCCATG ACCGCAAAAG CTTGCAATTG CATGAGCAGG TTCACAAAAA TATTGAGCAG 
CCTCTTAAAA ACAAAAACTG CAATGAAAAC AACTTTCCAT CTGTCACCAA GACAAGCTTC 
CTGCAATGCC TAAACTTGAA ACCTGCAGGA AATATTGAGA ACCCTAGTGA GGGAACCATA 
GGGAAAAGAA TCCCAGAGCT TGATCCTGTA AGCAGCTACC AAGCTTGGCA ACTGGCAACA 
AGAGGCAAAT TGGTGGAGGT AACAGAGAGA AGCCAGGGCT GGGAAGAAAG GTTAGCTGAT 
GCGGATGTAG CATACGACAG GGAAAAAGGC GAGTATGTCT TACTCAGACA GGACAAGCGG 
AAGAAATCAC TTGATTCCAC TGTGAGTATC CCAACCAAGA AACGGAGAGG GGCTGTTACC 
CAAGGCTGCA CTCCAGATCA GCACAGCAAT GGAGAAAAGA ACAGCCATGT TTCAACAGGT 
GAGCGAAGCC CTGAAAATCT GAGTGACTCT GAGTATCGCC CTACTTCTCG CCCTAGCCGT 
AAAAATTCCC AGAAAACTTC AGAGTGCTTG GAGTGTGGAA AGGGCTTCCG CACACAGCAA 
CAGATGGTGA TCCACATGCT CATCAGACAT GGTGGCTTGG GTGAAACCAT TGGTGGAAAT 
GGACTGTTCC GCAAAACAAC GTCTAGCCCA TCTAAAACAG GGGAATCTGC AGGACACTTC 
AGGGATCAAA GACGGGCTGG GTTCTTGGGG GACACAGATA AAAAGCCATA CACCTGTGAG 
CACTGTGACT TTTGCACCTC GGAGCCCTCA GCCATCGCTG CCCATATCCA AACGCATCAA 
TCGGCGGTCA GGGACTGGGG CCAGAGGAGT GATGCCTTAA CCAGCTCACT CAGCCAAAGC 
CAAACTCACC AAACCAATCA CACAGGCTTC CCAAGGCTGA GAAATGCTCT TCTCCAACAG 
CCCTACTGGC CCTACACCAC CTCAACTCAC CTGGAGAGGG CAGCACTGAG TGACGCCGCT 
TTAAAGACAG AGGAAGAAAA GAGCAAGGGT TTGGAGGAAA GCAGCTCCAC AGATAAAGCG 
GAGTCTAATC TGCTTAATCT CTCAGTGGAC GTGGATAACA AAAAAGAAGT TTCGTCCATT 
CTTTTGAACA ATTTGGTTAG ACACCAGTGC CCTTATTGCT CCTATGCAAC ACACTACCCA 
GAAGTTCTCT GGATCCACCA ACGAATCGCA CACAAAATTG ACAGCACTAC GTTGGTGCCG 
AAGTGGGCCC CAAAAAATGG CCTTAAGGGG CCCAAAACGC TTCTTGATTT CAAGAGACGC 
ACCGGGCCAC CTCCATTTCT GGAGGGTAAG GACTGCCCGT CTCTGCCTCA GATGAGAAGT 
TACAGAACCA GTCCACCTGA TTGCAGCCCT GGAGGGACGA AGAAATCAAA ACCTCTGACA 
AGTACTGTAG AGTCCAGCTC TTCGCAAAAC AAGAGCTGGC ACACAGCCAC AGCGCCAGGG 
GCATCATCGC ACTCGTCCAA ACACAAAACC AGCAAGTCCA GGACAGATGA GTTGGCAGGC 
AGTAAACACA AAACAGACAT CCATCAAAAC AACTCCTCAC GGCCCATGGC AAGTCCACAG 
AAGACTGGAA ACCCAAAGAC AGGTAGCCGA GTGGTGGAGA GTAGTTTGCT ACCTCAAGAG 
GGACTTCATT TTATGCTCTC TAGCAAACAC AACCTCTCGG ATCAGAGGAG CTCCAAAGCT 
CACGCTCCTC AGACCCCCAG TAAGTCTGAT TCTCAAGCTC AGGATACACC GTCTGTGGCT 
GGGTACGACC CCTGGAACAG ACTGGGCCTG AGTGGCCCGT CACCTCACTC TCAGTCCAAG 
AGACAGTCAA CAGCCGATGG TGCAGAAGGT GTGACGGACA TCCTGAGTTT TTTAAAGAAC 
TGTAACCCTC ACGATCTTGC TGCCCTCTAC CACCACTGGG GCTACAGTAG TGCCATGACA 
GAGCAAGCAG GGTTGGCGAG GTCAGGGTCT CAGCAGGGGG AGTACGTGTG TCCGGTGTGC 
AGGAAGAGCT TCAACCAACC CAGCCATTAC CGCACACACA TGAGATCACA CACAGTGATG 
TTTGAGTCCA ATGGACTCAT GGGAACTGGA GTCCATCCTC TACAGAAGCC CCCAAACAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Melanoma genome evolution across species.
BMC genomics18(1)136(2017 Feb)
Kansler ER,Verma A,Langdon EM,Simon-Vermot T,Yin A,Lee W,Attiyeh M,Elemento O,White RM

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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znf516 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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