mylk5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mylk5
Entrez Gene ID	100003770
Full Name	myosin, light chain kinase 5
Synonyms	im:7148400,si:dkey-194j22.6
General protein information	Preferred Names myosin, light chain kinase 5 Names myosin light chain kinase, smooth muscle mlck
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003198128.5	XP_003198176.2	myosin light chain kinase, smooth muscle isoform X2
XM_017354784.1	XP_017210273.1	myosin light chain kinase, smooth muscle isoform X4
XM_005164056.3	XP_005164113.1	myosin light chain kinase, smooth muscle isoform X1
XM_017354783.1	XP_017210272.1	myosin light chain kinase, smooth muscle isoform X2
XM_003198128.4	XP_003198176.2	myosin light chain kinase, smooth muscle isoform X3
XM_005164056.4	XP_005164113.1	myosin light chain kinase, smooth muscle isoform X1

Pathways from BioSystems
Gene Ontology

The following mylk5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mylk5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08482	XM_005164056.4 Latest version!	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa08484	XM_003198128.5 Latest version!	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa58242	XM_017354783.1 Latest version!	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa58243	XM_017354784.1 Latest version!	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa08484	XM_003198128.4	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa08482	XM_005164056.3	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mylk5 ( XM_005164056.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164113.1 Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08482
Clone ID Related Accession (Same CDS sequence)	XM_005164056.3 , XM_005164056.4
Accession Version	XM_005164056.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin light chain kinase, smooth muscle isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACTG CTGATGGAAA GACACGCTTT GTGTCCACTT TAAGGATACA ATTGTCTGGC 
TTCCAGCCAA ATGGACAGGG GTCTCGACCA GTCATAAAAA AGCCTGAAGA CACATCAAGT 
GCAGTGAATG GCAATTACAA CCACACAAGG CAACTGGATC CACCTCTTTT CACCAAGCCC 
CTCGAGGACT GTATAGTAGA TGAAGGAAAT GACATCACAT TGAACGGAAG TATCACTGGG 
AGCCAGCCAA TCAGCATTTC CTGGCTTCAT AATGGCGAGA CTGTACGATT TGGTAAATCT 
TCATTAGTGG GAGAAAAAGT GACTTTGGTA GTGAGGGAAT GTCTGCCAGA AGATGCTGGG 
GCTTATACCT GCATGGCTGA AAACATAGCC GGAAAAACAT CCAGCAGTGC AGCAGTTTGT 
GTAAGAGACT ATGAATCAAT TGTTAGAGTC AACAACAATA TCATCAACAA ATCCCACGCT 
CATCCTCACA CTCCTGACCT GCTGAACGGG ACATCAGAGG ACACAAGTGT TTGTAAAGGC 
CCAGAGTCTC CTCAGACAGG AAGTCCTGTT ACAAACTCTC CAGTACTCAA AAGAGAAGTG 
CATGTCAAGC GGAGGAGCAG CTCAGGTGCA GGTTTTACAG CACCTCCTCT GCAGTTTGTG 
GATCCATTAG ACCGGTTGGA GGTGCAAGTC GGAGAAACAG CCCGTCTAGA GTGTTTCTTC 
AAAGGCAGCT CACCTGTAGC ATCTTGCTGG GTCTTTAACA AAAAAGAGGT GGAGGAAAGC 
TCCAGGTTTC ATCTCGAGTC CTGTGATGAA AACAGTTCTC TGGTCATTTC TGAAGCTCTT 
CCTGAAGACT CTGGGCGTTA CACAATATTT GTACGAGATC GCAAAAGTTC CGCCCAACAC 
ACAGTGACAC TGAATGTCAT TGACCTGCCT CAGCCGCCTG CTTCTTGTCC AGTGGCGCGT 
GTTCTGTCCC CTCACTCTCT GGTTCTTTCC TGGTCTGGAC CATCGTATGA TGGTGGCAGC 
GCTGTGACGG GATACGTGGT GGAGGTTTGT GAAAAAGAGT CTGAACAGTG GAAAGAGCTC 
ACAGCGCAGT GTAAGAGCAC CTCTCTCAGG GTGACCTCTG GCCTCCAGCC TCAGGGTCAA 
TACTGCTTCC GCATCAGAGC CTGTAACACT GTGGGGGTCA GCGAACCCAG CCAAGAGTCC 
CAGACAGTCA CGATGGACAA CCCAGGAGAT TCCCGTGAAG AGAAGCCAGT GGAGTATGTG 
AATGTGACCA TCGACACTCA ACATAAACTC ACTGACCACT ATAACGTGCT GGAGAAACTA 
GGAGTAGGGA AGTTTGGCCA AGTGTTTCGG ATGACCCATA AGGTGACGGG TCGCGAATGT 
GCTGGAAAGT TCTATAAGAG TCGTCGGGCA AAGGACAGAG AGGCTGCGAG GAAAGAAATA 
GAGCTGATGA ACTTCTTACA CCATCCTAAA CTGGTGCAGT GTCTCGCTGC ATATGACAAC 
AAAGCCGAGA TGGTCATGGT GATGGAGTAT GTTGCTGGTG GTGAGCTGTT TGAGCGGATT 
GTCGATGACA GTTTTGAGCA CACAGAGGTG TCCAGTGTGG GCTACATGCG TCAGATTCTA 
GAGGGCATTA AGTACATGCA CCAGCAAAAC ATCCTCCATC TGGATCTCAA ACCTGAGAAT 
ATCGTCTGTG TGGACAGAAC CGGGAGTCAG ATCAAAATCA TTGACTTCGG ACTCGCTTGC 
AAACTAGATC CTAACACGCC TCTCAAGGTG ATGCAGGGCA CGCCAGAGTT TGTGGCCCCG 
GAGGTCATCA ACTTTGAGGC CGTCACTCTG ACCACCGATA TGTGGAGCGT CGGGGTGATC 
TGCTACATCC TGTTGAGCGG CGAGTCTCCG TTTCAGGGCG AGAGCGACAC CGAGACGTTG 
GCTTTGGTGA CCGCGGCGCA GTGGGAGTTT GATGAGGAGA GTTTTGAGGA GATCACTGAT 
ATGGCAAAAG GCTTCATCAG CTCCCTGCTC AATAAAGATC CCAGGCGCAG ATTGTCCTGT 
GAAGAGGCTT TGGCTCATGA CTGGCTGGGT TTTTTGTGCG TGGACAACAG CAGCAGCGGC 
GGCAAAAACC TCTCCAAAGA CAAGATGAAG AAGTTCCTGA CCAGGCAGAA GTGGAAGAAA 
ACAGGAAAAG CCTTGCTGGC TCTGAAGAGG ATGGCCATCT TGTCTAAACC AGAAGCTTCT 
GCAAGCACGA GCCCTGTCCA TGAGACAGAA CAGGCACTGC AGTCTCTGGA GCTGAAGCTT 
CAATGTAAAC CTGAGTTCAG TAAGACTCCA TCTGACCTGA GCGTCCCGCA GGGCTCCAGC 
GTCCATCTCT CCTGCCTCAT CACAGGTTAC CCTGACCCTG AGGTGGTGTG GTTGAGGGAC 
GGAGAGCTGC TGGAGCTGCA GGGCGGCTCT GTGACAGTGG ACTATGAGGA GGACGGCAGC 
TGTACTCTCA TCCTAAACAA TGTGTCTTTG AATCACAGCG GCCTGTATTC CTGTAAAGCC 
ACTAATGCAC TCGGGGAGGC GCTGTGCTCT GCTACACTTA CTGTAGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164113.1
CDS	174..2744
Translation	MSTADGKTRFVSTLRIQLSGFQPNGQGSRPVIKKPEDTSSAVNGNYNHTRQLDPPLFTKPLEDCIVDEGNDITLNGSITGSQPISISWLHNGETVRFGKSSLVGEKVTLVVRECLPEDAGAYTCMAENIAGKTSSSAAVCVRDYESIVRVNNNIINKSHAHPHTPDLLNGTSEDTSVCKGPESPQTGSPVTNSPVLKREVHVKRRSSSGAGFTAPPLQFVDPLDRLEVQVGETARLECFFKGSSPVASCWVFNKKEVEESSRFHLESCDENSSLVISEALPEDSGRYTIFVRDRKSSAQHTVTLNVIDLPQPPASCPVARVLSPHSLVLSWSGPSYDGGSAVTGYVVEVCEKESEQWKELTAQCKSTSLRVTSGLQPQGQYCFRIRACNTVGVSEPSQESQTVTMDNPGDSREEKPVEYVNVTIDTQHKLTDHYNVLEKLGVGKFGQVFRMTHKVTGRECAGKFYKSRRAKDREAARKEIELMNFLHHPKLVQCLAAYDNKAEMVMVMEYVAGGELFERIVDDSFEHTEVSSVGYMRQILEGIKYMHQQNILHLDLKPENIVCVDRTGSQIKIIDFGLACKLDPNTPLKVMQGTPEFVAPEVINFEAVTLTTDMWSVGVICYILLSGESPFQGESDTETLALVTAAQWEFDEESFEEITDMAKGFISSLLNKDPRRRLSCEEALAHDWLGFLCVDNSSSGGKNLSKDKMKKFLTRQKWKKTGKALLALKRMAILSKPEASASTSPVHETEQALQSLELKLQCKPEFSKTPSDLSVPQGSSVHLSCLITGYPDPEVVWLRDGELLELQGGSVTVDYEEDGSCTLILNNVSLNHSGLYSCKATNALGEALCSATLTVV

Target ORF information:

RefSeq Version	XM_005164056.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164056.3

ORF Insert Sequence:

ATGAGCACTG CTGATGGAAA GACACGCTTT GTGTCCACTT TAAGGATACA ATTGTCTGGC 
TTCCAGCCAA ATGGACAGGG GTCTCGACCA GTCATAAAAA AGCCTGAAGA CACATCAAGT 
GCAGTGAATG GCAATTACAA CCACACAAGG CAACTGGATC CACCTCTTTT CACCAAGCCC 
CTCGAGGACT GTATAGTAGA TGAAGGAAAT GACATCACAT TGAACGGAAG TATCACTGGG 
AGCCAGCCAA TCAGCATTTC CTGGCTTCAT AATGGCGAGA CTGTACGATT TGGTAAATCT 
TCATTAGTGG GAGAAAAAGT GACTTTGGTA GTGAGGGAAT GTCTGCCAGA AGATGCTGGG 
GCTTATACCT GCATGGCTGA AAACATAGCC GGAAAAACAT CCAGCAGTGC AGCAGTTTGT 
GTAAGAGACT ATGAATCAAT TGTTAGAGTC AACAACAATA TCATCAACAA ATCCCACGCT 
CATCCTCACA CTCCTGACCT GCTGAACGGG ACATCAGAGG ACACAAGTGT TTGTAAAGGC 
CCAGAGTCTC CTCAGACAGG AAGTCCTGTT ACAAACTCTC CAGTACTCAA AAGAGAAGTG 
CATGTCAAGC GGAGGAGCAG CTCAGGTGCA GGTTTTACAG CACCTCCTCT GCAGTTTGTG 
GATCCATTAG ACCGGTTGGA GGTGCAAGTC GGAGAAACAG CCCGTCTAGA GTGTTTCTTC 
AAAGGCAGCT CACCTGTAGC ATCTTGCTGG GTCTTTAACA AAAAAGAGGT GGAGGAAAGC 
TCCAGGTTTC ATCTCGAGTC CTGTGATGAA AACAGTTCTC TGGTCATTTC TGAAGCTCTT 
CCTGAAGACT CTGGGCGTTA CACAATATTT GTACGAGATC GCAAAAGTTC CGCCCAACAC 
ACAGTGACAC TGAATGTCAT TGACCTGCCT CAGCCGCCTG CTTCTTGTCC AGTGGCGCGT 
GTTCTGTCCC CTCACTCTCT GGTTCTTTCC TGGTCTGGAC CATCGTATGA TGGTGGCAGC 
GCTGTGACGG GATACGTGGT GGAGGTTTGT GAAAAAGAGT CTGAACAGTG GAAAGAGCTC 
ACAGCGCAGT GTAAGAGCAC CTCTCTCAGG GTGACCTCTG GCCTCCAGCC TCAGGGTCAA 
TACTGCTTCC GCATCAGAGC CTGTAACACT GTGGGGGTCA GCGAACCCAG CCAAGAGTCC 
CAGACAGTCA CGATGGACAA CCCAGGAGAT TCCCGTGAAG AGAAGCCAGT GGAGTATGTG 
AATGTGACCA TCGACACTCA ACATAAACTC ACTGACCACT ATAACGTGCT GGAGAAACTA 
GGAGTAGGGA AGTTTGGCCA AGTGTTTCGG ATGACCCATA AGGTGACGGG TCGCGAATGT 
GCTGGAAAGT TCTATAAGAG TCGTCGGGCA AAGGACAGAG AGGCTGCGAG GAAAGAAATA 
GAGCTGATGA ACTTCTTACA CCATCCTAAA CTGGTGCAGT GTCTCGCTGC ATATGACAAC 
AAAGCCGAGA TGGTCATGGT GATGGAGTAT GTTGCTGGTG GTGAGCTGTT TGAGCGGATT 
GTCGATGACA GTTTTGAGCA CACAGAGGTG TCCAGTGTGG GCTACATGCG TCAGATTCTA 
GAGGGCATTA AGTACATGCA CCAGCAAAAC ATCCTCCATC TGGATCTCAA ACCTGAGAAT 
ATCGTCTGTG TGGACAGAAC CGGGAGTCAG ATCAAAATCA TTGACTTCGG ACTCGCTTGC 
AAACTAGATC CTAACACGCC TCTCAAGGTG ATGCAGGGCA CGCCAGAGTT TGTGGCCCCG 
GAGGTCATCA ACTTTGAGGC CGTCACTCTG ACCACCGATA TGTGGAGCGT CGGGGTGATC 
TGCTACATCC TGTTGAGCGG CGAGTCTCCG TTTCAGGGCG AGAGCGACAC CGAGACGTTG 
GCTTTGGTGA CCGCGGCGCA GTGGGAGTTT GATGAGGAGA GTTTTGAGGA GATCACTGAT 
ATGGCAAAAG GCTTCATCAG CTCCCTGCTC AATAAAGATC CCAGGCGCAG ATTGTCCTGT 
GAAGAGGCTT TGGCTCATGA CTGGCTGGGT TTTTTGTGCG TGGACAACAG CAGCAGCGGC 
GGCAAAAACC TCTCCAAAGA CAAGATGAAG AAGTTCCTGA CCAGGCAGAA GTGGAAGAAA 
ACAGGAAAAG CCTTGCTGGC TCTGAAGAGG ATGGCCATCT TGTCTAAACC AGAAGCTTCT 
GCAAGCACGA GCCCTGTCCA TGAGACAGAA CAGGCACTGC AGTCTCTGGA GCTGAAGCTT 
CAATGTAAAC CTGAGTTCAG TAAGACTCCA TCTGACCTGA GCGTCCCGCA GGGCTCCAGC 
GTCCATCTCT CCTGCCTCAT CACAGGTTAC CCTGACCCTG AGGTGGTGTG GTTGAGGGAC 
GGAGAGCTGC TGGAGCTGCA GGGCGGCTCT GTGACAGTGG ACTATGAGGA GGACGGCAGC 
TGTACTCTCA TCCTAAACAA TGTGTCTTTG AATCACAGCG GCCTGTATTC CTGTAAAGCC 
ACTAATGCAC TCGGGGAGGC GCTGTGCTCT GCTACACTTA CTGTAGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mylk5 ( XM_005164056.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164113.1 Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08482
Clone ID Related Accession (Same CDS sequence)	XM_005164056.3 , XM_005164056.4
Accession Version	XM_005164056.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin light chain kinase, smooth muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164056.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACTG CTGATGGAAA GACACGCTTT GTGTCCACTT TAAGGATACA ATTGTCTGGC 
TTCCAGCCAA ATGGACAGGG GTCTCGACCA GTCATAAAAA AGCCTGAAGA CACATCAAGT 
GCAGTGAATG GCAATTACAA CCACACAAGG CAACTGGATC CACCTCTTTT CACCAAGCCC 
CTCGAGGACT GTATAGTAGA TGAAGGAAAT GACATCACAT TGAACGGAAG TATCACTGGG 
AGCCAGCCAA TCAGCATTTC CTGGCTTCAT AATGGCGAGA CTGTACGATT TGGTAAATCT 
TCATTAGTGG GAGAAAAAGT GACTTTGGTA GTGAGGGAAT GTCTGCCAGA AGATGCTGGG 
GCTTATACCT GCATGGCTGA AAACATAGCC GGAAAAACAT CCAGCAGTGC AGCAGTTTGT 
GTAAGAGACT ATGAATCAAT TGTTAGAGTC AACAACAATA TCATCAACAA ATCCCACGCT 
CATCCTCACA CTCCTGACCT GCTGAACGGG ACATCAGAGG ACACAAGTGT TTGTAAAGGC 
CCAGAGTCTC CTCAGACAGG AAGTCCTGTT ACAAACTCTC CAGTACTCAA AAGAGAAGTG 
CATGTCAAGC GGAGGAGCAG CTCAGGTGCA GGTTTTACAG CACCTCCTCT GCAGTTTGTG 
GATCCATTAG ACCGGTTGGA GGTGCAAGTC GGAGAAACAG CCCGTCTAGA GTGTTTCTTC 
AAAGGCAGCT CACCTGTAGC ATCTTGCTGG GTCTTTAACA AAAAAGAGGT GGAGGAAAGC 
TCCAGGTTTC ATCTCGAGTC CTGTGATGAA AACAGTTCTC TGGTCATTTC TGAAGCTCTT 
CCTGAAGACT CTGGGCGTTA CACAATATTT GTACGAGATC GCAAAAGTTC CGCCCAACAC 
ACAGTGACAC TGAATGTCAT TGACCTGCCT CAGCCGCCTG CTTCTTGTCC AGTGGCGCGT 
GTTCTGTCCC CTCACTCTCT GGTTCTTTCC TGGTCTGGAC CATCGTATGA TGGTGGCAGC 
GCTGTGACGG GATACGTGGT GGAGGTTTGT GAAAAAGAGT CTGAACAGTG GAAAGAGCTC 
ACAGCGCAGT GTAAGAGCAC CTCTCTCAGG GTGACCTCTG GCCTCCAGCC TCAGGGTCAA 
TACTGCTTCC GCATCAGAGC CTGTAACACT GTGGGGGTCA GCGAACCCAG CCAAGAGTCC 
CAGACAGTCA CGATGGACAA CCCAGGAGAT TCCCGTGAAG AGAAGCCAGT GGAGTATGTG 
AATGTGACCA TCGACACTCA ACATAAACTC ACTGACCACT ATAACGTGCT GGAGAAACTA 
GGAGTAGGGA AGTTTGGCCA AGTGTTTCGG ATGACCCATA AGGTGACGGG TCGCGAATGT 
GCTGGAAAGT TCTATAAGAG TCGTCGGGCA AAGGACAGAG AGGCTGCGAG GAAAGAAATA 
GAGCTGATGA ACTTCTTACA CCATCCTAAA CTGGTGCAGT GTCTCGCTGC ATATGACAAC 
AAAGCCGAGA TGGTCATGGT GATGGAGTAT GTTGCTGGTG GTGAGCTGTT TGAGCGGATT 
GTCGATGACA GTTTTGAGCA CACAGAGGTG TCCAGTGTGG GCTACATGCG TCAGATTCTA 
GAGGGCATTA AGTACATGCA CCAGCAAAAC ATCCTCCATC TGGATCTCAA ACCTGAGAAT 
ATCGTCTGTG TGGACAGAAC CGGGAGTCAG ATCAAAATCA TTGACTTCGG ACTCGCTTGC 
AAACTAGATC CTAACACGCC TCTCAAGGTG ATGCAGGGCA CGCCAGAGTT TGTGGCCCCG 
GAGGTCATCA ACTTTGAGGC CGTCACTCTG ACCACCGATA TGTGGAGCGT CGGGGTGATC 
TGCTACATCC TGTTGAGCGG CGAGTCTCCG TTTCAGGGCG AGAGCGACAC CGAGACGTTG 
GCTTTGGTGA CCGCGGCGCA GTGGGAGTTT GATGAGGAGA GTTTTGAGGA GATCACTGAT 
ATGGCAAAAG GCTTCATCAG CTCCCTGCTC AATAAAGATC CCAGGCGCAG ATTGTCCTGT 
GAAGAGGCTT TGGCTCATGA CTGGCTGGGT TTTTTGTGCG TGGACAACAG CAGCAGCGGC 
GGCAAAAACC TCTCCAAAGA CAAGATGAAG AAGTTCCTGA CCAGGCAGAA GTGGAAGAAA 
ACAGGAAAAG CCTTGCTGGC TCTGAAGAGG ATGGCCATCT TGTCTAAACC AGAAGCTTCT 
GCAAGCACGA GCCCTGTCCA TGAGACAGAA CAGGCACTGC AGTCTCTGGA GCTGAAGCTT 
CAATGTAAAC CTGAGTTCAG TAAGACTCCA TCTGACCTGA GCGTCCCGCA GGGCTCCAGC 
GTCCATCTCT CCTGCCTCAT CACAGGTTAC CCTGACCCTG AGGTGGTGTG GTTGAGGGAC 
GGAGAGCTGC TGGAGCTGCA GGGCGGCTCT GTGACAGTGG ACTATGAGGA GGACGGCAGC 
TGTACTCTCA TCCTAAACAA TGTGTCTTTG AATCACAGCG GCCTGTATTC CTGTAAAGCC 
ACTAATGCAC TCGGGGAGGC GCTGTGCTCT GCTACACTTA CTGTAGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164113.1
CDS	151..2721
Translation	MSTADGKTRFVSTLRIQLSGFQPNGQGSRPVIKKPEDTSSAVNGNYNHTRQLDPPLFTKPLEDCIVDEGNDITLNGSITGSQPISISWLHNGETVRFGKSSLVGEKVTLVVRECLPEDAGAYTCMAENIAGKTSSSAAVCVRDYESIVRVNNNIINKSHAHPHTPDLLNGTSEDTSVCKGPESPQTGSPVTNSPVLKREVHVKRRSSSGAGFTAPPLQFVDPLDRLEVQVGETARLECFFKGSSPVASCWVFNKKEVEESSRFHLESCDENSSLVISEALPEDSGRYTIFVRDRKSSAQHTVTLNVIDLPQPPASCPVARVLSPHSLVLSWSGPSYDGGSAVTGYVVEVCEKESEQWKELTAQCKSTSLRVTSGLQPQGQYCFRIRACNTVGVSEPSQESQTVTMDNPGDSREEKPVEYVNVTIDTQHKLTDHYNVLEKLGVGKFGQVFRMTHKVTGRECAGKFYKSRRAKDREAARKEIELMNFLHHPKLVQCLAAYDNKAEMVMVMEYVAGGELFERIVDDSFEHTEVSSVGYMRQILEGIKYMHQQNILHLDLKPENIVCVDRTGSQIKIIDFGLACKLDPNTPLKVMQGTPEFVAPEVINFEAVTLTTDMWSVGVICYILLSGESPFQGESDTETLALVTAAQWEFDEESFEEITDMAKGFISSLLNKDPRRRLSCEEALAHDWLGFLCVDNSSSGGKNLSKDKMKKFLTRQKWKKTGKALLALKRMAILSKPEASASTSPVHETEQALQSLELKLQCKPEFSKTPSDLSVPQGSSVHLSCLITGYPDPEVVWLRDGELLELQGGSVTVDYEEDGSCTLILNNVSLNHSGLYSCKATNALGEALCSATLTVV

Target ORF information:

RefSeq Version	XM_005164056.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164056.4

ORF Insert Sequence:

ATGAGCACTG CTGATGGAAA GACACGCTTT GTGTCCACTT TAAGGATACA ATTGTCTGGC 
TTCCAGCCAA ATGGACAGGG GTCTCGACCA GTCATAAAAA AGCCTGAAGA CACATCAAGT 
GCAGTGAATG GCAATTACAA CCACACAAGG CAACTGGATC CACCTCTTTT CACCAAGCCC 
CTCGAGGACT GTATAGTAGA TGAAGGAAAT GACATCACAT TGAACGGAAG TATCACTGGG 
AGCCAGCCAA TCAGCATTTC CTGGCTTCAT AATGGCGAGA CTGTACGATT TGGTAAATCT 
TCATTAGTGG GAGAAAAAGT GACTTTGGTA GTGAGGGAAT GTCTGCCAGA AGATGCTGGG 
GCTTATACCT GCATGGCTGA AAACATAGCC GGAAAAACAT CCAGCAGTGC AGCAGTTTGT 
GTAAGAGACT ATGAATCAAT TGTTAGAGTC AACAACAATA TCATCAACAA ATCCCACGCT 
CATCCTCACA CTCCTGACCT GCTGAACGGG ACATCAGAGG ACACAAGTGT TTGTAAAGGC 
CCAGAGTCTC CTCAGACAGG AAGTCCTGTT ACAAACTCTC CAGTACTCAA AAGAGAAGTG 
CATGTCAAGC GGAGGAGCAG CTCAGGTGCA GGTTTTACAG CACCTCCTCT GCAGTTTGTG 
GATCCATTAG ACCGGTTGGA GGTGCAAGTC GGAGAAACAG CCCGTCTAGA GTGTTTCTTC 
AAAGGCAGCT CACCTGTAGC ATCTTGCTGG GTCTTTAACA AAAAAGAGGT GGAGGAAAGC 
TCCAGGTTTC ATCTCGAGTC CTGTGATGAA AACAGTTCTC TGGTCATTTC TGAAGCTCTT 
CCTGAAGACT CTGGGCGTTA CACAATATTT GTACGAGATC GCAAAAGTTC CGCCCAACAC 
ACAGTGACAC TGAATGTCAT TGACCTGCCT CAGCCGCCTG CTTCTTGTCC AGTGGCGCGT 
GTTCTGTCCC CTCACTCTCT GGTTCTTTCC TGGTCTGGAC CATCGTATGA TGGTGGCAGC 
GCTGTGACGG GATACGTGGT GGAGGTTTGT GAAAAAGAGT CTGAACAGTG GAAAGAGCTC 
ACAGCGCAGT GTAAGAGCAC CTCTCTCAGG GTGACCTCTG GCCTCCAGCC TCAGGGTCAA 
TACTGCTTCC GCATCAGAGC CTGTAACACT GTGGGGGTCA GCGAACCCAG CCAAGAGTCC 
CAGACAGTCA CGATGGACAA CCCAGGAGAT TCCCGTGAAG AGAAGCCAGT GGAGTATGTG 
AATGTGACCA TCGACACTCA ACATAAACTC ACTGACCACT ATAACGTGCT GGAGAAACTA 
GGAGTAGGGA AGTTTGGCCA AGTGTTTCGG ATGACCCATA AGGTGACGGG TCGCGAATGT 
GCTGGAAAGT TCTATAAGAG TCGTCGGGCA AAGGACAGAG AGGCTGCGAG GAAAGAAATA 
GAGCTGATGA ACTTCTTACA CCATCCTAAA CTGGTGCAGT GTCTCGCTGC ATATGACAAC 
AAAGCCGAGA TGGTCATGGT GATGGAGTAT GTTGCTGGTG GTGAGCTGTT TGAGCGGATT 
GTCGATGACA GTTTTGAGCA CACAGAGGTG TCCAGTGTGG GCTACATGCG TCAGATTCTA 
GAGGGCATTA AGTACATGCA CCAGCAAAAC ATCCTCCATC TGGATCTCAA ACCTGAGAAT 
ATCGTCTGTG TGGACAGAAC CGGGAGTCAG ATCAAAATCA TTGACTTCGG ACTCGCTTGC 
AAACTAGATC CTAACACGCC TCTCAAGGTG ATGCAGGGCA CGCCAGAGTT TGTGGCCCCG 
GAGGTCATCA ACTTTGAGGC CGTCACTCTG ACCACCGATA TGTGGAGCGT CGGGGTGATC 
TGCTACATCC TGTTGAGCGG CGAGTCTCCG TTTCAGGGCG AGAGCGACAC CGAGACGTTG 
GCTTTGGTGA CCGCGGCGCA GTGGGAGTTT GATGAGGAGA GTTTTGAGGA GATCACTGAT 
ATGGCAAAAG GCTTCATCAG CTCCCTGCTC AATAAAGATC CCAGGCGCAG ATTGTCCTGT 
GAAGAGGCTT TGGCTCATGA CTGGCTGGGT TTTTTGTGCG TGGACAACAG CAGCAGCGGC 
GGCAAAAACC TCTCCAAAGA CAAGATGAAG AAGTTCCTGA CCAGGCAGAA GTGGAAGAAA 
ACAGGAAAAG CCTTGCTGGC TCTGAAGAGG ATGGCCATCT TGTCTAAACC AGAAGCTTCT 
GCAAGCACGA GCCCTGTCCA TGAGACAGAA CAGGCACTGC AGTCTCTGGA GCTGAAGCTT 
CAATGTAAAC CTGAGTTCAG TAAGACTCCA TCTGACCTGA GCGTCCCGCA GGGCTCCAGC 
GTCCATCTCT CCTGCCTCAT CACAGGTTAC CCTGACCCTG AGGTGGTGTG GTTGAGGGAC 
GGAGAGCTGC TGGAGCTGCA GGGCGGCTCT GTGACAGTGG ACTATGAGGA GGACGGCAGC 
TGTACTCTCA TCCTAAACAA TGTGTCTTTG AATCACAGCG GCCTGTATTC CTGTAAAGCC 
ACTAATGCAC TCGGGGAGGC GCTGTGCTCT GCTACACTTA CTGTAGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mylk5 ( XM_003198128.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198176.2 Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa08484
Clone ID Related Accession (Same CDS sequence)	XM_003198128.5 , XM_003198128.4
Accession Version	XM_003198128.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin light chain kinase, smooth muscle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003198128.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACTG CTGATGGAAA GACACGCTTT GTGTCCACTT TAAGGATACA ATTGTCTGGC 
TTCCAGCCAA ATGGACAGGG GTCTCGACCA GTCATAAAAA AGCCTGAAGA CACATCAAGT 
GCAGTGAATG GCAATTACAA CCACACAAGG CAACTGGATC CACCTCTTTT CACCAAGCCC 
CTCGAGGACT GTATAGTAGA TGAAGGAAAT GACATCACAT TGAACGGAAG TATCACTGGG 
AGCCAGCCAA TCAGCATTTC CTGGCTTCAT AATGACTATG AATCAATTGT TAGAGTCAAC 
AACAATATCA TCAACAAATC CCACGCTCAT CCTCACACTC CTGACCTGCT GAACGGGACA 
TCAGAGGACA CAAGTGTTTG TAAAGGCCCA GAGTCTCCTC AGACAGGAAG TCCTGTTACA 
AACTCTCCAG TACTCAAAAG AGAAGTGCAT GTCAAGCGGA GGAGCAGCTC AGGTGCAGGT 
TTTACAGCAC CTCCTCTGCA GTTTGTGGAT CCATTAGACC GGTTGGAGGT GCAAGTCGGA 
GAAACAGCCC GTCTAGAGTG TTTCTTCAAA GGCAGCTCAC CTGTAGCATC TTGCTGGGTC 
TTTAACAAAA AAGAGGTGGA GGAAAGCTCC AGGTTTCATC TCGAGTCCTG TGATGAAAAC 
AGTTCTCTGG TCATTTCTGA AGCTCTTCCT GAAGACTCTG GGCGTTACAC AATATTTGTA 
CGAGATCGCA AAAGTTCCGC CCAACACACA GTGACACTGA ATGTCATTGA CCTGCCTCAG 
CCGCCTGCTT CTTGTCCAGT GGCGCGTGTT CTGTCCCCTC ACTCTCTGGT TCTTTCCTGG 
TCTGGACCAT CGTATGATGG TGGCAGCGCT GTGACGGGAT ACGTGGTGGA GGTTTGTGAA 
AAAGAGTCTG AACAGTGGAA AGAGCTCACA GCGCAGTGTA AGAGCACCTC TCTCAGGGTG 
ACCTCTGGCC TCCAGCCTCA GGGTCAATAC TGCTTCCGCA TCAGAGCCTG TAACACTGTG 
GGGGTCAGCG AACCCAGCCA AGAGTCCCAG ACAGTCACGA TGGACAACCC AGGAGATTCC 
CGTGAAGAGA AGCCAGTGGA GTATGTGAAT GTGACCATCG ACACTCAACA TAAACTCACT 
GACCACTATA ACGTGCTGGA GAAACTAGGA GTAGGGAAGT TTGGCCAAGT GTTTCGGATG 
ACCCATAAGG TGACGGGTCG CGAATGTGCT GGAAAGTTCT ATAAGAGTCG TCGGGCAAAG 
GACAGAGAGG CTGCGAGGAA AGAAATAGAG CTGATGAACT TCTTACACCA TCCTAAACTG 
GTGCAGTGTC TCGCTGCATA TGACAACAAA GCCGAGATGG TCATGGTGAT GGAGTATGTT 
GCTGGTGGTG AGCTGTTTGA GCGGATTGTC GATGACAGTT TTGAGCACAC AGAGGTGTCC 
AGTGTGGGCT ACATGCGTCA GATTCTAGAG GGCATTAAGT ACATGCACCA GCAAAACATC 
CTCCATCTGG ATCTCAAACC TGAGAATATC GTCTGTGTGG ACAGAACCGG GAGTCAGATC 
AAAATCATTG ACTTCGGACT CGCTTGCAAA CTAGATCCTA ACACGCCTCT CAAGGTGATG 
CAGGGCACGC CAGAGTTTGT GGCCCCGGAG GTCATCAACT TTGAGGCCGT CACTCTGACC 
ACCGATATGT GGAGCGTCGG GGTGATCTGC TACATCCTGT TGAGCGGCGA GTCTCCGTTT 
CAGGGCGAGA GCGACACCGA GACGTTGGCT TTGGTGACCG CGGCGCAGTG GGAGTTTGAT 
GAGGAGAGTT TTGAGGAGAT CACTGATATG GCAAAAGGCT TCATCAGCTC CCTGCTCAAT 
AAAGATCCCA GGCGCAGATT GTCCTGTGAA GAGGCTTTGG CTCATGACTG GCTGGGTTTT 
TTGTGCGTGG ACAACAGCAG CAGCGGCGGC AAAAACCTCT CCAAAGACAA GATGAAGAAG 
TTCCTGACCA GGCAGAAGTG GAAGAAAACA GGAAAAGCCT TGCTGGCTCT GAAGAGGATG 
GCCATCTTGT CTAAACCAGA AGCTTCTGCA AGCACGAGCC CTGTCCATGA GACAGAACAG 
GCACTGCAGT CTCTGGAGCT GAAGCTTCAA TGTAAACCTG AGTTCAGTAA GACTCCATCT 
GACCTGAGCG TCCCGCAGGG CTCCAGCGTC CATCTCTCCT GCCTCATCAC AGGTTACCCT 
GACCCTGAGG TGGTGTGGTT GAGGGACGGA GAGCTGCTGG AGCTGCAGGG CGGCTCTGTG 
ACAGTGGACT ATGAGGAGGA CGGCAGCTGT ACTCTCATCC TAAACAATGT GTCTTTGAAT 
CACAGCGGCC TGTATTCCTG TAAAGCCACT AATGCACTCG GGGAGGCGCT GTGCTCTGCT 
ACACTTACTG TAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198176.2
CDS	150..2567
Translation	MSTADGKTRFVSTLRIQLSGFQPNGQGSRPVIKKPEDTSSAVNGNYNHTRQLDPPLFTKPLEDCIVDEGNDITLNGSITGSQPISISWLHNDYESIVRVNNNIINKSHAHPHTPDLLNGTSEDTSVCKGPESPQTGSPVTNSPVLKREVHVKRRSSSGAGFTAPPLQFVDPLDRLEVQVGETARLECFFKGSSPVASCWVFNKKEVEESSRFHLESCDENSSLVISEALPEDSGRYTIFVRDRKSSAQHTVTLNVIDLPQPPASCPVARVLSPHSLVLSWSGPSYDGGSAVTGYVVEVCEKESEQWKELTAQCKSTSLRVTSGLQPQGQYCFRIRACNTVGVSEPSQESQTVTMDNPGDSREEKPVEYVNVTIDTQHKLTDHYNVLEKLGVGKFGQVFRMTHKVTGRECAGKFYKSRRAKDREAARKEIELMNFLHHPKLVQCLAAYDNKAEMVMVMEYVAGGELFERIVDDSFEHTEVSSVGYMRQILEGIKYMHQQNILHLDLKPENIVCVDRTGSQIKIIDFGLACKLDPNTPLKVMQGTPEFVAPEVINFEAVTLTTDMWSVGVICYILLSGESPFQGESDTETLALVTAAQWEFDEESFEEITDMAKGFISSLLNKDPRRRLSCEEALAHDWLGFLCVDNSSSGGKNLSKDKMKKFLTRQKWKKTGKALLALKRMAILSKPEASASTSPVHETEQALQSLELKLQCKPEFSKTPSDLSVPQGSSVHLSCLITGYPDPEVVWLRDGELLELQGGSVTVDYEEDGSCTLILNNVSLNHSGLYSCKATNALGEALCSATLTVV

Target ORF information:

RefSeq Version	XM_003198128.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198128.5

ORF Insert Sequence:

ATGAGCACTG CTGATGGAAA GACACGCTTT GTGTCCACTT TAAGGATACA ATTGTCTGGC 
TTCCAGCCAA ATGGACAGGG GTCTCGACCA GTCATAAAAA AGCCTGAAGA CACATCAAGT 
GCAGTGAATG GCAATTACAA CCACACAAGG CAACTGGATC CACCTCTTTT CACCAAGCCC 
CTCGAGGACT GTATAGTAGA TGAAGGAAAT GACATCACAT TGAACGGAAG TATCACTGGG 
AGCCAGCCAA TCAGCATTTC CTGGCTTCAT AATGACTATG AATCAATTGT TAGAGTCAAC 
AACAATATCA TCAACAAATC CCACGCTCAT CCTCACACTC CTGACCTGCT GAACGGGACA 
TCAGAGGACA CAAGTGTTTG TAAAGGCCCA GAGTCTCCTC AGACAGGAAG TCCTGTTACA 
AACTCTCCAG TACTCAAAAG AGAAGTGCAT GTCAAGCGGA GGAGCAGCTC AGGTGCAGGT 
TTTACAGCAC CTCCTCTGCA GTTTGTGGAT CCATTAGACC GGTTGGAGGT GCAAGTCGGA 
GAAACAGCCC GTCTAGAGTG TTTCTTCAAA GGCAGCTCAC CTGTAGCATC TTGCTGGGTC 
TTTAACAAAA AAGAGGTGGA GGAAAGCTCC AGGTTTCATC TCGAGTCCTG TGATGAAAAC 
AGTTCTCTGG TCATTTCTGA AGCTCTTCCT GAAGACTCTG GGCGTTACAC AATATTTGTA 
CGAGATCGCA AAAGTTCCGC CCAACACACA GTGACACTGA ATGTCATTGA CCTGCCTCAG 
CCGCCTGCTT CTTGTCCAGT GGCGCGTGTT CTGTCCCCTC ACTCTCTGGT TCTTTCCTGG 
TCTGGACCAT CGTATGATGG TGGCAGCGCT GTGACGGGAT ACGTGGTGGA GGTTTGTGAA 
AAAGAGTCTG AACAGTGGAA AGAGCTCACA GCGCAGTGTA AGAGCACCTC TCTCAGGGTG 
ACCTCTGGCC TCCAGCCTCA GGGTCAATAC TGCTTCCGCA TCAGAGCCTG TAACACTGTG 
GGGGTCAGCG AACCCAGCCA AGAGTCCCAG ACAGTCACGA TGGACAACCC AGGAGATTCC 
CGTGAAGAGA AGCCAGTGGA GTATGTGAAT GTGACCATCG ACACTCAACA TAAACTCACT 
GACCACTATA ACGTGCTGGA GAAACTAGGA GTAGGGAAGT TTGGCCAAGT GTTTCGGATG 
ACCCATAAGG TGACGGGTCG CGAATGTGCT GGAAAGTTCT ATAAGAGTCG TCGGGCAAAG 
GACAGAGAGG CTGCGAGGAA AGAAATAGAG CTGATGAACT TCTTACACCA TCCTAAACTG 
GTGCAGTGTC TCGCTGCATA TGACAACAAA GCCGAGATGG TCATGGTGAT GGAGTATGTT 
GCTGGTGGTG AGCTGTTTGA GCGGATTGTC GATGACAGTT TTGAGCACAC AGAGGTGTCC 
AGTGTGGGCT ACATGCGTCA GATTCTAGAG GGCATTAAGT ACATGCACCA GCAAAACATC 
CTCCATCTGG ATCTCAAACC TGAGAATATC GTCTGTGTGG ACAGAACCGG GAGTCAGATC 
AAAATCATTG ACTTCGGACT CGCTTGCAAA CTAGATCCTA ACACGCCTCT CAAGGTGATG 
CAGGGCACGC CAGAGTTTGT GGCCCCGGAG GTCATCAACT TTGAGGCCGT CACTCTGACC 
ACCGATATGT GGAGCGTCGG GGTGATCTGC TACATCCTGT TGAGCGGCGA GTCTCCGTTT 
CAGGGCGAGA GCGACACCGA GACGTTGGCT TTGGTGACCG CGGCGCAGTG GGAGTTTGAT 
GAGGAGAGTT TTGAGGAGAT CACTGATATG GCAAAAGGCT TCATCAGCTC CCTGCTCAAT 
AAAGATCCCA GGCGCAGATT GTCCTGTGAA GAGGCTTTGG CTCATGACTG GCTGGGTTTT 
TTGTGCGTGG ACAACAGCAG CAGCGGCGGC AAAAACCTCT CCAAAGACAA GATGAAGAAG 
TTCCTGACCA GGCAGAAGTG GAAGAAAACA GGAAAAGCCT TGCTGGCTCT GAAGAGGATG 
GCCATCTTGT CTAAACCAGA AGCTTCTGCA AGCACGAGCC CTGTCCATGA GACAGAACAG 
GCACTGCAGT CTCTGGAGCT GAAGCTTCAA TGTAAACCTG AGTTCAGTAA GACTCCATCT 
GACCTGAGCG TCCCGCAGGG CTCCAGCGTC CATCTCTCCT GCCTCATCAC AGGTTACCCT 
GACCCTGAGG TGGTGTGGTT GAGGGACGGA GAGCTGCTGG AGCTGCAGGG CGGCTCTGTG 
ACAGTGGACT ATGAGGAGGA CGGCAGCTGT ACTCTCATCC TAAACAATGT GTCTTTGAAT 
CACAGCGGCC TGTATTCCTG TAAAGCCACT AATGCACTCG GGGAGGCGCT GTGCTCTGCT 
ACACTTACTG TAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mylk5 ( XM_003198128.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198176.2 Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa08484
Clone ID Related Accession (Same CDS sequence)	XM_003198128.5 , XM_003198128.4
Accession Version	XM_003198128.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin light chain kinase, smooth muscle isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003198128.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACTG CTGATGGAAA GACACGCTTT GTGTCCACTT TAAGGATACA ATTGTCTGGC 
TTCCAGCCAA ATGGACAGGG GTCTCGACCA GTCATAAAAA AGCCTGAAGA CACATCAAGT 
GCAGTGAATG GCAATTACAA CCACACAAGG CAACTGGATC CACCTCTTTT CACCAAGCCC 
CTCGAGGACT GTATAGTAGA TGAAGGAAAT GACATCACAT TGAACGGAAG TATCACTGGG 
AGCCAGCCAA TCAGCATTTC CTGGCTTCAT AATGACTATG AATCAATTGT TAGAGTCAAC 
AACAATATCA TCAACAAATC CCACGCTCAT CCTCACACTC CTGACCTGCT GAACGGGACA 
TCAGAGGACA CAAGTGTTTG TAAAGGCCCA GAGTCTCCTC AGACAGGAAG TCCTGTTACA 
AACTCTCCAG TACTCAAAAG AGAAGTGCAT GTCAAGCGGA GGAGCAGCTC AGGTGCAGGT 
TTTACAGCAC CTCCTCTGCA GTTTGTGGAT CCATTAGACC GGTTGGAGGT GCAAGTCGGA 
GAAACAGCCC GTCTAGAGTG TTTCTTCAAA GGCAGCTCAC CTGTAGCATC TTGCTGGGTC 
TTTAACAAAA AAGAGGTGGA GGAAAGCTCC AGGTTTCATC TCGAGTCCTG TGATGAAAAC 
AGTTCTCTGG TCATTTCTGA AGCTCTTCCT GAAGACTCTG GGCGTTACAC AATATTTGTA 
CGAGATCGCA AAAGTTCCGC CCAACACACA GTGACACTGA ATGTCATTGA CCTGCCTCAG 
CCGCCTGCTT CTTGTCCAGT GGCGCGTGTT CTGTCCCCTC ACTCTCTGGT TCTTTCCTGG 
TCTGGACCAT CGTATGATGG TGGCAGCGCT GTGACGGGAT ACGTGGTGGA GGTTTGTGAA 
AAAGAGTCTG AACAGTGGAA AGAGCTCACA GCGCAGTGTA AGAGCACCTC TCTCAGGGTG 
ACCTCTGGCC TCCAGCCTCA GGGTCAATAC TGCTTCCGCA TCAGAGCCTG TAACACTGTG 
GGGGTCAGCG AACCCAGCCA AGAGTCCCAG ACAGTCACGA TGGACAACCC AGGAGATTCC 
CGTGAAGAGA AGCCAGTGGA GTATGTGAAT GTGACCATCG ACACTCAACA TAAACTCACT 
GACCACTATA ACGTGCTGGA GAAACTAGGA GTAGGGAAGT TTGGCCAAGT GTTTCGGATG 
ACCCATAAGG TGACGGGTCG CGAATGTGCT GGAAAGTTCT ATAAGAGTCG TCGGGCAAAG 
GACAGAGAGG CTGCGAGGAA AGAAATAGAG CTGATGAACT TCTTACACCA TCCTAAACTG 
GTGCAGTGTC TCGCTGCATA TGACAACAAA GCCGAGATGG TCATGGTGAT GGAGTATGTT 
GCTGGTGGTG AGCTGTTTGA GCGGATTGTC GATGACAGTT TTGAGCACAC AGAGGTGTCC 
AGTGTGGGCT ACATGCGTCA GATTCTAGAG GGCATTAAGT ACATGCACCA GCAAAACATC 
CTCCATCTGG ATCTCAAACC TGAGAATATC GTCTGTGTGG ACAGAACCGG GAGTCAGATC 
AAAATCATTG ACTTCGGACT CGCTTGCAAA CTAGATCCTA ACACGCCTCT CAAGGTGATG 
CAGGGCACGC CAGAGTTTGT GGCCCCGGAG GTCATCAACT TTGAGGCCGT CACTCTGACC 
ACCGATATGT GGAGCGTCGG GGTGATCTGC TACATCCTGT TGAGCGGCGA GTCTCCGTTT 
CAGGGCGAGA GCGACACCGA GACGTTGGCT TTGGTGACCG CGGCGCAGTG GGAGTTTGAT 
GAGGAGAGTT TTGAGGAGAT CACTGATATG GCAAAAGGCT TCATCAGCTC CCTGCTCAAT 
AAAGATCCCA GGCGCAGATT GTCCTGTGAA GAGGCTTTGG CTCATGACTG GCTGGGTTTT 
TTGTGCGTGG ACAACAGCAG CAGCGGCGGC AAAAACCTCT CCAAAGACAA GATGAAGAAG 
TTCCTGACCA GGCAGAAGTG GAAGAAAACA GGAAAAGCCT TGCTGGCTCT GAAGAGGATG 
GCCATCTTGT CTAAACCAGA AGCTTCTGCA AGCACGAGCC CTGTCCATGA GACAGAACAG 
GCACTGCAGT CTCTGGAGCT GAAGCTTCAA TGTAAACCTG AGTTCAGTAA GACTCCATCT 
GACCTGAGCG TCCCGCAGGG CTCCAGCGTC CATCTCTCCT GCCTCATCAC AGGTTACCCT 
GACCCTGAGG TGGTGTGGTT GAGGGACGGA GAGCTGCTGG AGCTGCAGGG CGGCTCTGTG 
ACAGTGGACT ATGAGGAGGA CGGCAGCTGT ACTCTCATCC TAAACAATGT GTCTTTGAAT 
CACAGCGGCC TGTATTCCTG TAAAGCCACT AATGCACTCG GGGAGGCGCT GTGCTCTGCT 
ACACTTACTG TAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198176.2
CDS	173..2590
Translation	MSTADGKTRFVSTLRIQLSGFQPNGQGSRPVIKKPEDTSSAVNGNYNHTRQLDPPLFTKPLEDCIVDEGNDITLNGSITGSQPISISWLHNDYESIVRVNNNIINKSHAHPHTPDLLNGTSEDTSVCKGPESPQTGSPVTNSPVLKREVHVKRRSSSGAGFTAPPLQFVDPLDRLEVQVGETARLECFFKGSSPVASCWVFNKKEVEESSRFHLESCDENSSLVISEALPEDSGRYTIFVRDRKSSAQHTVTLNVIDLPQPPASCPVARVLSPHSLVLSWSGPSYDGGSAVTGYVVEVCEKESEQWKELTAQCKSTSLRVTSGLQPQGQYCFRIRACNTVGVSEPSQESQTVTMDNPGDSREEKPVEYVNVTIDTQHKLTDHYNVLEKLGVGKFGQVFRMTHKVTGRECAGKFYKSRRAKDREAARKEIELMNFLHHPKLVQCLAAYDNKAEMVMVMEYVAGGELFERIVDDSFEHTEVSSVGYMRQILEGIKYMHQQNILHLDLKPENIVCVDRTGSQIKIIDFGLACKLDPNTPLKVMQGTPEFVAPEVINFEAVTLTTDMWSVGVICYILLSGESPFQGESDTETLALVTAAQWEFDEESFEEITDMAKGFISSLLNKDPRRRLSCEEALAHDWLGFLCVDNSSSGGKNLSKDKMKKFLTRQKWKKTGKALLALKRMAILSKPEASASTSPVHETEQALQSLELKLQCKPEFSKTPSDLSVPQGSSVHLSCLITGYPDPEVVWLRDGELLELQGGSVTVDYEEDGSCTLILNNVSLNHSGLYSCKATNALGEALCSATLTVV

Target ORF information:

RefSeq Version	XM_003198128.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198128.4

ORF Insert Sequence:

ATGAGCACTG CTGATGGAAA GACACGCTTT GTGTCCACTT TAAGGATACA ATTGTCTGGC 
TTCCAGCCAA ATGGACAGGG GTCTCGACCA GTCATAAAAA AGCCTGAAGA CACATCAAGT 
GCAGTGAATG GCAATTACAA CCACACAAGG CAACTGGATC CACCTCTTTT CACCAAGCCC 
CTCGAGGACT GTATAGTAGA TGAAGGAAAT GACATCACAT TGAACGGAAG TATCACTGGG 
AGCCAGCCAA TCAGCATTTC CTGGCTTCAT AATGACTATG AATCAATTGT TAGAGTCAAC 
AACAATATCA TCAACAAATC CCACGCTCAT CCTCACACTC CTGACCTGCT GAACGGGACA 
TCAGAGGACA CAAGTGTTTG TAAAGGCCCA GAGTCTCCTC AGACAGGAAG TCCTGTTACA 
AACTCTCCAG TACTCAAAAG AGAAGTGCAT GTCAAGCGGA GGAGCAGCTC AGGTGCAGGT 
TTTACAGCAC CTCCTCTGCA GTTTGTGGAT CCATTAGACC GGTTGGAGGT GCAAGTCGGA 
GAAACAGCCC GTCTAGAGTG TTTCTTCAAA GGCAGCTCAC CTGTAGCATC TTGCTGGGTC 
TTTAACAAAA AAGAGGTGGA GGAAAGCTCC AGGTTTCATC TCGAGTCCTG TGATGAAAAC 
AGTTCTCTGG TCATTTCTGA AGCTCTTCCT GAAGACTCTG GGCGTTACAC AATATTTGTA 
CGAGATCGCA AAAGTTCCGC CCAACACACA GTGACACTGA ATGTCATTGA CCTGCCTCAG 
CCGCCTGCTT CTTGTCCAGT GGCGCGTGTT CTGTCCCCTC ACTCTCTGGT TCTTTCCTGG 
TCTGGACCAT CGTATGATGG TGGCAGCGCT GTGACGGGAT ACGTGGTGGA GGTTTGTGAA 
AAAGAGTCTG AACAGTGGAA AGAGCTCACA GCGCAGTGTA AGAGCACCTC TCTCAGGGTG 
ACCTCTGGCC TCCAGCCTCA GGGTCAATAC TGCTTCCGCA TCAGAGCCTG TAACACTGTG 
GGGGTCAGCG AACCCAGCCA AGAGTCCCAG ACAGTCACGA TGGACAACCC AGGAGATTCC 
CGTGAAGAGA AGCCAGTGGA GTATGTGAAT GTGACCATCG ACACTCAACA TAAACTCACT 
GACCACTATA ACGTGCTGGA GAAACTAGGA GTAGGGAAGT TTGGCCAAGT GTTTCGGATG 
ACCCATAAGG TGACGGGTCG CGAATGTGCT GGAAAGTTCT ATAAGAGTCG TCGGGCAAAG 
GACAGAGAGG CTGCGAGGAA AGAAATAGAG CTGATGAACT TCTTACACCA TCCTAAACTG 
GTGCAGTGTC TCGCTGCATA TGACAACAAA GCCGAGATGG TCATGGTGAT GGAGTATGTT 
GCTGGTGGTG AGCTGTTTGA GCGGATTGTC GATGACAGTT TTGAGCACAC AGAGGTGTCC 
AGTGTGGGCT ACATGCGTCA GATTCTAGAG GGCATTAAGT ACATGCACCA GCAAAACATC 
CTCCATCTGG ATCTCAAACC TGAGAATATC GTCTGTGTGG ACAGAACCGG GAGTCAGATC 
AAAATCATTG ACTTCGGACT CGCTTGCAAA CTAGATCCTA ACACGCCTCT CAAGGTGATG 
CAGGGCACGC CAGAGTTTGT GGCCCCGGAG GTCATCAACT TTGAGGCCGT CACTCTGACC 
ACCGATATGT GGAGCGTCGG GGTGATCTGC TACATCCTGT TGAGCGGCGA GTCTCCGTTT 
CAGGGCGAGA GCGACACCGA GACGTTGGCT TTGGTGACCG CGGCGCAGTG GGAGTTTGAT 
GAGGAGAGTT TTGAGGAGAT CACTGATATG GCAAAAGGCT TCATCAGCTC CCTGCTCAAT 
AAAGATCCCA GGCGCAGATT GTCCTGTGAA GAGGCTTTGG CTCATGACTG GCTGGGTTTT 
TTGTGCGTGG ACAACAGCAG CAGCGGCGGC AAAAACCTCT CCAAAGACAA GATGAAGAAG 
TTCCTGACCA GGCAGAAGTG GAAGAAAACA GGAAAAGCCT TGCTGGCTCT GAAGAGGATG 
GCCATCTTGT CTAAACCAGA AGCTTCTGCA AGCACGAGCC CTGTCCATGA GACAGAACAG 
GCACTGCAGT CTCTGGAGCT GAAGCTTCAA TGTAAACCTG AGTTCAGTAA GACTCCATCT 
GACCTGAGCG TCCCGCAGGG CTCCAGCGTC CATCTCTCCT GCCTCATCAC AGGTTACCCT 
GACCCTGAGG TGGTGTGGTT GAGGGACGGA GAGCTGCTGG AGCTGCAGGG CGGCTCTGTG 
ACAGTGGACT ATGAGGAGGA CGGCAGCTGT ACTCTCATCC TAAACAATGT GTCTTTGAAT 
CACAGCGGCC TGTATTCCTG TAAAGCCACT AATGCACTCG GGGAGGCGCT GTGCTCTGCT 
ACACTTACTG TAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mylk5 ( XM_017354783.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210272.1 Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa58242
Clone ID Related Accession (Same CDS sequence)	XM_017354783.1
Accession Version	XM_017354783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin light chain kinase, smooth muscle isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACTG CTGATGGAAA GACACGCTTT GTGTCCACTT TAAGGATACA ATTGTCTGGC 
TTCCAGCCAA ATGGACAGGG GTCTCGACCA GTCATAAAAA AGCCTGAAGA CACATCAAGT 
GCAGTGAATG GCAATTACAA CCACACAAGG CAACTGGATC CACCTCTTTT CACCAAGCCC 
CTCGAGGACT GTATAGTAGA TGAAGGAAAT GACATCACAT TGAACGGAAG TATCACTGGG 
AGCCAGCCAA TCAGCATTTC CTGGCTTCAT AATGGCGAGA CTGTACGATT TGGTAAATCT 
TCATTAGTGG GAGAAAAAGT GACTTTGGTA GTGAGGGAAT GTCTGCCAGA AGATGCTGGG 
GCTTATACCT GCATGGCTGA AAACATAGCC GGAAAAACAT CCAGCAGTGC AGCAGTTTGT 
GTAAGAGACT ATGAATCAAT TGTTAGAGTC AACAACAATA TCATCAACAA ATCCCACGCT 
CATCCTCACA CTCCTGACCT GCTGAACGGG ACATCAGAGG ACACAAGTGT TTGTAAAGGC 
CCAGAGTCTC CTCAGACAGG AAGTCCTGTT ACAAACTCTC CAGTACTCAA AAGAGAAGTG 
CATGTCAAGC GGAGGAGCAG CTCAGGTGCA GGTTTTACAG CACCTCCTCT GCAGTTTGTG 
GATCCATTAG ACCGGTTGGA GGTGCAAGTC GGAGAAACAG CCCGTCTAGA GTGTTTCTTC 
AAAGGCAGCT CACCTGTAGC ATCTTGCTGG GTCTTTAACA AAAAAGAGGT GGAGGAAAGC 
TCCAGGTTTC ATCTCGAGTC CTGTGATGAA AACAGTTCTC TGGTCATTTC TGAAGCTCTT 
CCTGAAGACT CTGGGCGTTA CACAATATTT GTACGAGATC GCAAAAGTTC CGCCCAACAC 
ACAGTGACAC TGAATGTCAT TGACCTGCCT CAGCCGCCTG CTTCTTGTCC AGTGGCGCGT 
GTTCTGTCCC CTCACTCTCT GGTTCTTTCC TGGTCTGGAC CATCGTATGA TGGTGGCAGC 
GCTGTGACGG GATACGTGGT GGAGGTTTGT GAAAAAGAGT CTGAACAGTG GAAAGAGCTC 
ACAGCGCAGT GTAAGAGCAC CTCTCTCAGG GTGACCTCTG GCCTCCAGCC TCAGGGTCAA 
TACTGCTTCC GCATCAGAGC CTGTAACACT GTGGGGGTCA GCGAACCCAG CCAAGAGTCC 
CAGACAGTCA CGATGGACAA CCCAGATTCC CGTGAAGAGA AGCCAGTGGA GTATGTGAAT 
GTGACCATCG ACACTCAACA TAAACTCACT GACCACTATA ACGTGCTGGA GAAACTAGGA 
GTAGGGAAGT TTGGCCAAGT GTTTCGGATG ACCCATAAGG TGACGGGTCG CGAATGTGCT 
GGAAAGTTCT ATAAGAGTCG TCGGGCAAAG GACAGAGAGG CTGCGAGGAA AGAAATAGAG 
CTGATGAACT TCTTACACCA TCCTAAACTG GTGCAGTGTC TCGCTGCATA TGACAACAAA 
GCCGAGATGG TCATGGTGAT GGAGTATGTT GCTGGTGGTG AGCTGTTTGA GCGGATTGTC 
GATGACAGTT TTGAGCACAC AGAGGTGTCC AGTGTGGGCT ACATGCGTCA GATTCTAGAG 
GGCATTAAGT ACATGCACCA GCAAAACATC CTCCATCTGG ATCTCAAACC TGAGAATATC 
GTCTGTGTGG ACAGAACCGG GAGTCAGATC AAAATCATTG ACTTCGGACT CGCTTGCAAA 
CTAGATCCTA ACACGCCTCT CAAGGTGATG CAGGGCACGC CAGAGTTTGT GGCCCCGGAG 
GTCATCAACT TTGAGGCCGT CACTCTGACC ACCGATATGT GGAGCGTCGG GGTGATCTGC 
TACATCCTGT TGAGCGGCGA GTCTCCGTTT CAGGGCGAGA GCGACACCGA GACGTTGGCT 
TTGGTGACCG CGGCGCAGTG GGAGTTTGAT GAGGAGAGTT TTGAGGAGAT CACTGATATG 
GCAAAAGGCT TCATCAGCTC CCTGCTCAAT AAAGATCCCA GGCGCAGATT GTCCTGTGAA 
GAGGCTTTGG CTCATGACTG GCTGGGTTTT TTGTGCGTGG ACAACAGCAG CAGCGGCGGC 
AAAAACCTCT CCAAAGACAA GATGAAGAAG TTCCTGACCA GGCAGAAGTG GAAGAAAACA 
GGAAAAGCCT TGCTGGCTCT GAAGAGGATG GCCATCTTGT CTAAACCAGA AGCTTCTGCA 
AGCACGAGCC CTGTCCATGA GACAGAACAG GCACTGCAGT CTCTGGAGCT GAAGCTTCAA 
TGTAAACCTG AGTTCAGTAA GACTCCATCT GACCTGAGCG TCCCGCAGGG CTCCAGCGTC 
CATCTCTCCT GCCTCATCAC AGGTTACCCT GACCCTGAGG TGGTGTGGTT GAGGGACGGA 
GAGCTGCTGG AGCTGCAGGG CGGCTCTGTG ACAGTGGACT ATGAGGAGGA CGGCAGCTGT 
ACTCTCATCC TAAACAATGT GTCTTTGAAT CACAGCGGCC TGTATTCCTG TAAAGCCACT 
AATGCACTCG GGGAGGCGCT GTGCTCTGCT ACACTTACTG TAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210272.1
CDS	175..2742
Translation	MSTADGKTRFVSTLRIQLSGFQPNGQGSRPVIKKPEDTSSAVNGNYNHTRQLDPPLFTKPLEDCIVDEGNDITLNGSITGSQPISISWLHNGETVRFGKSSLVGEKVTLVVRECLPEDAGAYTCMAENIAGKTSSSAAVCVRDYESIVRVNNNIINKSHAHPHTPDLLNGTSEDTSVCKGPESPQTGSPVTNSPVLKREVHVKRRSSSGAGFTAPPLQFVDPLDRLEVQVGETARLECFFKGSSPVASCWVFNKKEVEESSRFHLESCDENSSLVISEALPEDSGRYTIFVRDRKSSAQHTVTLNVIDLPQPPASCPVARVLSPHSLVLSWSGPSYDGGSAVTGYVVEVCEKESEQWKELTAQCKSTSLRVTSGLQPQGQYCFRIRACNTVGVSEPSQESQTVTMDNPDSREEKPVEYVNVTIDTQHKLTDHYNVLEKLGVGKFGQVFRMTHKVTGRECAGKFYKSRRAKDREAARKEIELMNFLHHPKLVQCLAAYDNKAEMVMVMEYVAGGELFERIVDDSFEHTEVSSVGYMRQILEGIKYMHQQNILHLDLKPENIVCVDRTGSQIKIIDFGLACKLDPNTPLKVMQGTPEFVAPEVINFEAVTLTTDMWSVGVICYILLSGESPFQGESDTETLALVTAAQWEFDEESFEEITDMAKGFISSLLNKDPRRRLSCEEALAHDWLGFLCVDNSSSGGKNLSKDKMKKFLTRQKWKKTGKALLALKRMAILSKPEASASTSPVHETEQALQSLELKLQCKPEFSKTPSDLSVPQGSSVHLSCLITGYPDPEVVWLRDGELLELQGGSVTVDYEEDGSCTLILNNVSLNHSGLYSCKATNALGEALCSATLTVV

Target ORF information:

RefSeq Version	XM_017354783.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354783.1

ORF Insert Sequence:

ATGAGCACTG CTGATGGAAA GACACGCTTT GTGTCCACTT TAAGGATACA ATTGTCTGGC 
TTCCAGCCAA ATGGACAGGG GTCTCGACCA GTCATAAAAA AGCCTGAAGA CACATCAAGT 
GCAGTGAATG GCAATTACAA CCACACAAGG CAACTGGATC CACCTCTTTT CACCAAGCCC 
CTCGAGGACT GTATAGTAGA TGAAGGAAAT GACATCACAT TGAACGGAAG TATCACTGGG 
AGCCAGCCAA TCAGCATTTC CTGGCTTCAT AATGGCGAGA CTGTACGATT TGGTAAATCT 
TCATTAGTGG GAGAAAAAGT GACTTTGGTA GTGAGGGAAT GTCTGCCAGA AGATGCTGGG 
GCTTATACCT GCATGGCTGA AAACATAGCC GGAAAAACAT CCAGCAGTGC AGCAGTTTGT 
GTAAGAGACT ATGAATCAAT TGTTAGAGTC AACAACAATA TCATCAACAA ATCCCACGCT 
CATCCTCACA CTCCTGACCT GCTGAACGGG ACATCAGAGG ACACAAGTGT TTGTAAAGGC 
CCAGAGTCTC CTCAGACAGG AAGTCCTGTT ACAAACTCTC CAGTACTCAA AAGAGAAGTG 
CATGTCAAGC GGAGGAGCAG CTCAGGTGCA GGTTTTACAG CACCTCCTCT GCAGTTTGTG 
GATCCATTAG ACCGGTTGGA GGTGCAAGTC GGAGAAACAG CCCGTCTAGA GTGTTTCTTC 
AAAGGCAGCT CACCTGTAGC ATCTTGCTGG GTCTTTAACA AAAAAGAGGT GGAGGAAAGC 
TCCAGGTTTC ATCTCGAGTC CTGTGATGAA AACAGTTCTC TGGTCATTTC TGAAGCTCTT 
CCTGAAGACT CTGGGCGTTA CACAATATTT GTACGAGATC GCAAAAGTTC CGCCCAACAC 
ACAGTGACAC TGAATGTCAT TGACCTGCCT CAGCCGCCTG CTTCTTGTCC AGTGGCGCGT 
GTTCTGTCCC CTCACTCTCT GGTTCTTTCC TGGTCTGGAC CATCGTATGA TGGTGGCAGC 
GCTGTGACGG GATACGTGGT GGAGGTTTGT GAAAAAGAGT CTGAACAGTG GAAAGAGCTC 
ACAGCGCAGT GTAAGAGCAC CTCTCTCAGG GTGACCTCTG GCCTCCAGCC TCAGGGTCAA 
TACTGCTTCC GCATCAGAGC CTGTAACACT GTGGGGGTCA GCGAACCCAG CCAAGAGTCC 
CAGACAGTCA CGATGGACAA CCCAGATTCC CGTGAAGAGA AGCCAGTGGA GTATGTGAAT 
GTGACCATCG ACACTCAACA TAAACTCACT GACCACTATA ACGTGCTGGA GAAACTAGGA 
GTAGGGAAGT TTGGCCAAGT GTTTCGGATG ACCCATAAGG TGACGGGTCG CGAATGTGCT 
GGAAAGTTCT ATAAGAGTCG TCGGGCAAAG GACAGAGAGG CTGCGAGGAA AGAAATAGAG 
CTGATGAACT TCTTACACCA TCCTAAACTG GTGCAGTGTC TCGCTGCATA TGACAACAAA 
GCCGAGATGG TCATGGTGAT GGAGTATGTT GCTGGTGGTG AGCTGTTTGA GCGGATTGTC 
GATGACAGTT TTGAGCACAC AGAGGTGTCC AGTGTGGGCT ACATGCGTCA GATTCTAGAG 
GGCATTAAGT ACATGCACCA GCAAAACATC CTCCATCTGG ATCTCAAACC TGAGAATATC 
GTCTGTGTGG ACAGAACCGG GAGTCAGATC AAAATCATTG ACTTCGGACT CGCTTGCAAA 
CTAGATCCTA ACACGCCTCT CAAGGTGATG CAGGGCACGC CAGAGTTTGT GGCCCCGGAG 
GTCATCAACT TTGAGGCCGT CACTCTGACC ACCGATATGT GGAGCGTCGG GGTGATCTGC 
TACATCCTGT TGAGCGGCGA GTCTCCGTTT CAGGGCGAGA GCGACACCGA GACGTTGGCT 
TTGGTGACCG CGGCGCAGTG GGAGTTTGAT GAGGAGAGTT TTGAGGAGAT CACTGATATG 
GCAAAAGGCT TCATCAGCTC CCTGCTCAAT AAAGATCCCA GGCGCAGATT GTCCTGTGAA 
GAGGCTTTGG CTCATGACTG GCTGGGTTTT TTGTGCGTGG ACAACAGCAG CAGCGGCGGC 
AAAAACCTCT CCAAAGACAA GATGAAGAAG TTCCTGACCA GGCAGAAGTG GAAGAAAACA 
GGAAAAGCCT TGCTGGCTCT GAAGAGGATG GCCATCTTGT CTAAACCAGA AGCTTCTGCA 
AGCACGAGCC CTGTCCATGA GACAGAACAG GCACTGCAGT CTCTGGAGCT GAAGCTTCAA 
TGTAAACCTG AGTTCAGTAA GACTCCATCT GACCTGAGCG TCCCGCAGGG CTCCAGCGTC 
CATCTCTCCT GCCTCATCAC AGGTTACCCT GACCCTGAGG TGGTGTGGTT GAGGGACGGA 
GAGCTGCTGG AGCTGCAGGG CGGCTCTGTG ACAGTGGACT ATGAGGAGGA CGGCAGCTGT 
ACTCTCATCC TAAACAATGT GTCTTTGAAT CACAGCGGCC TGTATTCCTG TAAAGCCACT 
AATGCACTCG GGGAGGCGCT GTGCTCTGCT ACACTTACTG TAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mylk5 ( XM_017354784.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210273.1 Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X4, mRNA.

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CloneID	ODa58243
Clone ID Related Accession (Same CDS sequence)	XM_017354784.1
Accession Version	XM_017354784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin light chain kinase, smooth muscle isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCAA ATCAAAATGA ATCAAAAGAC TATGAATCAA TTGTTAGAGT CAACAACAAT 
ATCATCAACA AATCCCACGC TCATCCTCAC ACTCCTGACC TGCTGAACGG GACATCAGAG 
GACACAAGTG TTTGTAAAGG CCCAGAGTCT CCTCAGACAG GAAGTCCTGT TACAAACTCT 
CCAGTACTCA AAAGAGAAGT GCATGTCAAG CGGAGGAGCA GCTCAGGTGC AGGTTTTACA 
GCACCTCCTC TGCAGTTTGT GGATCCATTA GACCGGTTGG AGGTGCAAGT CGGAGAAACA 
GCCCGTCTAG AGTGTTTCTT CAAAGGCAGC TCACCTGTAG CATCTTGCTG GGTCTTTAAC 
AAAAAAGAGG TGGAGGAAAG CTCCAGGTTT CATCTCGAGT CCTGTGATGA AAACAGTTCT 
CTGGTCATTT CTGAAGCTCT TCCTGAAGAC TCTGGGCGTT ACACAATATT TGTACGAGAT 
CGCAAAAGTT CCGCCCAACA CACAGTGACA CTGAATGTCA TTGACCTGCC TCAGCCGCCT 
GCTTCTTGTC CAGTGGCGCG TGTTCTGTCC CCTCACTCTC TGGTTCTTTC CTGGTCTGGA 
CCATCGTATG ATGGTGGCAG CGCTGTGACG GGATACGTGG TGGAGGTTTG TGAAAAAGAG 
TCTGAACAGT GGAAAGAGCT CACAGCGCAG TGTAAGAGCA CCTCTCTCAG GGTGACCTCT 
GGCCTCCAGC CTCAGGGTCA ATACTGCTTC CGCATCAGAG CCTGTAACAC TGTGGGGGTC 
AGCGAACCCA GCCAAGAGTC CCAGACAGTC ACGATGGACA ACCCAGGAGA TTCCCGTGAA 
GAGAAGCCAG TGGAGTATGT GAATGTGACC ATCGACACTC AACATAAACT CACTGACCAC 
TATAACGTGC TGGAGAAACT AGGAGTAGGG AAGTTTGGCC AAGTGTTTCG GATGACCCAT 
AAGGTGACGG GTCGCGAATG TGCTGGAAAG TTCTATAAGA GTCGTCGGGC AAAGGACAGA 
GAGGCTGCGA GGAAAGAAAT AGAGCTGATG AACTTCTTAC ACCATCCTAA ACTGGTGCAG 
TGTCTCGCTG CATATGACAA CAAAGCCGAG ATGGTCATGG TGATGGAGTA TGTTGCTGGT 
GGTGAGCTGT TTGAGCGGAT TGTCGATGAC AGTTTTGAGC ACACAGAGGT GTCCAGTGTG 
GGCTACATGC GTCAGATTCT AGAGGGCATT AAGTACATGC ACCAGCAAAA CATCCTCCAT 
CTGGATCTCA AACCTGAGAA TATCGTCTGT GTGGACAGAA CCGGGAGTCA GATCAAAATC 
ATTGACTTCG GACTCGCTTG CAAACTAGAT CCTAACACGC CTCTCAAGGT GATGCAGGGC 
ACGCCAGAGT TTGTGGCCCC GGAGGTCATC AACTTTGAGG CCGTCACTCT GACCACCGAT 
ATGTGGAGCG TCGGGGTGAT CTGCTACATC CTGTTGAGCG GCGAGTCTCC GTTTCAGGGC 
GAGAGCGACA CCGAGACGTT GGCTTTGGTG ACCGCGGCGC AGTGGGAGTT TGATGAGGAG 
AGTTTTGAGG AGATCACTGA TATGGCAAAA GGCTTCATCA GCTCCCTGCT CAATAAAGAT 
CCCAGGCGCA GATTGTCCTG TGAAGAGGCT TTGGCTCATG ACTGGCTGGG TTTTTTGTGC 
GTGGACAACA GCAGCAGCGG CGGCAAAAAC CTCTCCAAAG ACAAGATGAA GAAGTTCCTG 
ACCAGGCAGA AGTGGAAGAA AACAGGAAAA GCCTTGCTGG CTCTGAAGAG GATGGCCATC 
TTGTCTAAAC CAGAAGCTTC TGCAAGCACG AGCCCTGTCC ATGAGACAGA ACAGGCACTG 
CAGTCTCTGG AGCTGAAGCT TCAATGTAAA CCTGAGTTCA GTAAGACTCC ATCTGACCTG 
AGCGTCCCGC AGGGCTCCAG CGTCCATCTC TCCTGCCTCA TCACAGGTTA CCCTGACCCT 
GAGGTGGTGT GGTTGAGGGA CGGAGAGCTG CTGGAGCTGC AGGGCGGCTC TGTGACAGTG 
GACTATGAGG AGGACGGCAG CTGTACTCTC ATCCTAAACA ATGTGTCTTT GAATCACAGC 
GGCCTGTATT CCTGTAAAGC CACTAATGCA CTCGGGGAGG CGCTGTGCTC TGCTACACTT 
ACTGTAGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210273.1
CDS	115..2286
Translation	MSPNQNESKDYESIVRVNNNIINKSHAHPHTPDLLNGTSEDTSVCKGPESPQTGSPVTNSPVLKREVHVKRRSSSGAGFTAPPLQFVDPLDRLEVQVGETARLECFFKGSSPVASCWVFNKKEVEESSRFHLESCDENSSLVISEALPEDSGRYTIFVRDRKSSAQHTVTLNVIDLPQPPASCPVARVLSPHSLVLSWSGPSYDGGSAVTGYVVEVCEKESEQWKELTAQCKSTSLRVTSGLQPQGQYCFRIRACNTVGVSEPSQESQTVTMDNPGDSREEKPVEYVNVTIDTQHKLTDHYNVLEKLGVGKFGQVFRMTHKVTGRECAGKFYKSRRAKDREAARKEIELMNFLHHPKLVQCLAAYDNKAEMVMVMEYVAGGELFERIVDDSFEHTEVSSVGYMRQILEGIKYMHQQNILHLDLKPENIVCVDRTGSQIKIIDFGLACKLDPNTPLKVMQGTPEFVAPEVINFEAVTLTTDMWSVGVICYILLSGESPFQGESDTETLALVTAAQWEFDEESFEEITDMAKGFISSLLNKDPRRRLSCEEALAHDWLGFLCVDNSSSGGKNLSKDKMKKFLTRQKWKKTGKALLALKRMAILSKPEASASTSPVHETEQALQSLELKLQCKPEFSKTPSDLSVPQGSSVHLSCLITGYPDPEVVWLRDGELLELQGGSVTVDYEEDGSCTLILNNVSLNHSGLYSCKATNALGEALCSATLTVV

Target ORF information:

RefSeq Version	XM_017354784.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin, light chain kinase 5 (mylk5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354784.1

ORF Insert Sequence:

ATGTCTCCAA ATCAAAATGA ATCAAAAGAC TATGAATCAA TTGTTAGAGT CAACAACAAT 
ATCATCAACA AATCCCACGC TCATCCTCAC ACTCCTGACC TGCTGAACGG GACATCAGAG 
GACACAAGTG TTTGTAAAGG CCCAGAGTCT CCTCAGACAG GAAGTCCTGT TACAAACTCT 
CCAGTACTCA AAAGAGAAGT GCATGTCAAG CGGAGGAGCA GCTCAGGTGC AGGTTTTACA 
GCACCTCCTC TGCAGTTTGT GGATCCATTA GACCGGTTGG AGGTGCAAGT CGGAGAAACA 
GCCCGTCTAG AGTGTTTCTT CAAAGGCAGC TCACCTGTAG CATCTTGCTG GGTCTTTAAC 
AAAAAAGAGG TGGAGGAAAG CTCCAGGTTT CATCTCGAGT CCTGTGATGA AAACAGTTCT 
CTGGTCATTT CTGAAGCTCT TCCTGAAGAC TCTGGGCGTT ACACAATATT TGTACGAGAT 
CGCAAAAGTT CCGCCCAACA CACAGTGACA CTGAATGTCA TTGACCTGCC TCAGCCGCCT 
GCTTCTTGTC CAGTGGCGCG TGTTCTGTCC CCTCACTCTC TGGTTCTTTC CTGGTCTGGA 
CCATCGTATG ATGGTGGCAG CGCTGTGACG GGATACGTGG TGGAGGTTTG TGAAAAAGAG 
TCTGAACAGT GGAAAGAGCT CACAGCGCAG TGTAAGAGCA CCTCTCTCAG GGTGACCTCT 
GGCCTCCAGC CTCAGGGTCA ATACTGCTTC CGCATCAGAG CCTGTAACAC TGTGGGGGTC 
AGCGAACCCA GCCAAGAGTC CCAGACAGTC ACGATGGACA ACCCAGGAGA TTCCCGTGAA 
GAGAAGCCAG TGGAGTATGT GAATGTGACC ATCGACACTC AACATAAACT CACTGACCAC 
TATAACGTGC TGGAGAAACT AGGAGTAGGG AAGTTTGGCC AAGTGTTTCG GATGACCCAT 
AAGGTGACGG GTCGCGAATG TGCTGGAAAG TTCTATAAGA GTCGTCGGGC AAAGGACAGA 
GAGGCTGCGA GGAAAGAAAT AGAGCTGATG AACTTCTTAC ACCATCCTAA ACTGGTGCAG 
TGTCTCGCTG CATATGACAA CAAAGCCGAG ATGGTCATGG TGATGGAGTA TGTTGCTGGT 
GGTGAGCTGT TTGAGCGGAT TGTCGATGAC AGTTTTGAGC ACACAGAGGT GTCCAGTGTG 
GGCTACATGC GTCAGATTCT AGAGGGCATT AAGTACATGC ACCAGCAAAA CATCCTCCAT 
CTGGATCTCA AACCTGAGAA TATCGTCTGT GTGGACAGAA CCGGGAGTCA GATCAAAATC 
ATTGACTTCG GACTCGCTTG CAAACTAGAT CCTAACACGC CTCTCAAGGT GATGCAGGGC 
ACGCCAGAGT TTGTGGCCCC GGAGGTCATC AACTTTGAGG CCGTCACTCT GACCACCGAT 
ATGTGGAGCG TCGGGGTGAT CTGCTACATC CTGTTGAGCG GCGAGTCTCC GTTTCAGGGC 
GAGAGCGACA CCGAGACGTT GGCTTTGGTG ACCGCGGCGC AGTGGGAGTT TGATGAGGAG 
AGTTTTGAGG AGATCACTGA TATGGCAAAA GGCTTCATCA GCTCCCTGCT CAATAAAGAT 
CCCAGGCGCA GATTGTCCTG TGAAGAGGCT TTGGCTCATG ACTGGCTGGG TTTTTTGTGC 
GTGGACAACA GCAGCAGCGG CGGCAAAAAC CTCTCCAAAG ACAAGATGAA GAAGTTCCTG 
ACCAGGCAGA AGTGGAAGAA AACAGGAAAA GCCTTGCTGG CTCTGAAGAG GATGGCCATC 
TTGTCTAAAC CAGAAGCTTC TGCAAGCACG AGCCCTGTCC ATGAGACAGA ACAGGCACTG 
CAGTCTCTGG AGCTGAAGCT TCAATGTAAA CCTGAGTTCA GTAAGACTCC ATCTGACCTG 
AGCGTCCCGC AGGGCTCCAG CGTCCATCTC TCCTGCCTCA TCACAGGTTA CCCTGACCCT 
GAGGTGGTGT GGTTGAGGGA CGGAGAGCTG CTGGAGCTGC AGGGCGGCTC TGTGACAGTG 
GACTATGAGG AGGACGGCAG CTGTACTCTC ATCCTAAACA ATGTGTCTTT GAATCACAGC 
GGCCTGTATT CCTGTAAAGC CACTAATGCA CTCGGGGAGG CGCTGTGCTC TGCTACACTT 
ACTGTAGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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mylk5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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