SI:DKEY-27P18.5 cDNA ORF clone, Danio rerio(Zebrafish)

The following SI:DKEY-27P18.5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:DKEY-27P18.5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa144549 XM_021471585.1
Latest version!
Danio rerio si:dkey-27p18.5 (si:dkey-27p18.5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa10392 XM_001342718.6
Latest version!
Danio rerio si:dkey-27p18.5 (si:dkey-27p18.5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa10392 XM_001342718.5 Danio rerio si:dkey-27p18.5 (si:dkey-27p18.5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa144549
    Clone ID Related Accession (Same CDS sequence) XM_021471585.1
    Accession Version XM_021471585.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin beta chain, non-erythrocytic 4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGATGC AGGTTCGGAA GGGGAGACTC GAGGAGGTTC TGGAAAGGGC AGAGGCTGTT 
    GCTGCCTTGC GGACCCCAGA GGTGGAGCTT GTGCGCGAGG GGGCGGGGCA TGTGAGACAG
    CTGTGGGAGG TGCTTCAGGT GGAGATGGAA CGCCGCACAG TGATGCTGGA TGCTGTGAAC
    CAAGCCCAGC AGTACTACAG CCAGGCAGCG AAGACAGAGT CCTGGCTCAG TGGACAGAAA
    CTTCAGGTCC TCAATGAGGA GAAAGGAAAC GACGAGGCCA GCACTCTTAG GTTACTGAAG
    GAGCAGTTGG CTTTAGAGCA AACAGTGGAG AATTATGCAG AGACAGTGGG CTCACTGTCC
    CAGCAGTGCC GTCGCATGCT TGAGTTGGGA CATCCAGACA GTGAGCAAAT CACCAAGCAG
    CAGGCTCATA TAGACCGGCT ATACGTATCT CTGAAAGACC TGGTAGAGCA GAGGAAAACA
    AAACTGGAGC AGCAGTACTG GTTATATCAG CTGAAGCGAG AGGTGGAGGC ACTGGAGAAA
    TGGATCTCTG AGAGGGAAGC TGTTGCCAGC TCCACTGAGC TCGGCCAGGA CCTGGAGCAT
    GTCACGGTTC TTCAGAGCAG CTTCACGCAG TTTTCCTCTG AGACACGTGC AGTGGGCCAG
    AAACAGATGG ATTCAGTCAA TAAGATGGTG AATGAGATGA TCGACTGCAG TCACTCTGAT
    GCTGCCACCA TTGCAGAGTG GAAAGACGGA CTGAACGAGT CCTGGGCGGA TCTACTGGAG
    CTCATGGAGA CCAGAGCGCA GATGTTGGCA GCATCCTACC AGCTGCACAA GTTCTTCACT
    GACTGTCAGG AGGTATTGGT ACAGATAGAA GGAAAGATGA GACAGCTGCC AGAGGTGAGG
    TCCTGCCAGG TGAGCTCGGC CAACCCTGGC ACACTACAGA GACTCCTGCA CTCTTTCGAA
    CACTCTCTGC AGCTGCTGGT CTCGCAGGTA CGCCAACTGC AAGAAAATGC AGTTCAGCTA
    CGTGCAATCT ATGCTGGGGA GAAGGCAGAT GCCATTTTGA GCCGTGAGCA GGAAGTGATG
    CAGGCCTGGA AAGAGCTCCT CGTTGCATGT GAGGGCAGCC GTGTGCAGGT CACCACTGTA
    ACCGATAAGA TCCAGTTCTT TGCGGTGGTG CGGGAATTGA GCATGTGGAT GGATGGAATC
    ATGGGGCAGA TTGGCCCAAA TGACGATGCA AGGGACCTCT CAGTGCTAGA GGGCATGATG
    TCCCAACATC AGAATCTGAA GAGTAAGATG GAAAACAAGA GCAAGAACTT TGTGCACTGC
    GTGGAGATGG GAAAAATGCT GCTCGCTGCG CGCAACCCTG CAGCTGAAGA GGTGAAGGAA
    AAGCTGGAGT ATGTCATGGC AAAGCAGAAA GAGATGAATG AGCGATGGGA CCTGCACTGG
    GAGAAGCTTC AGCAAGCCAA GCAGAGGCTG CAGTCCACTC AGACGGGATT GACGGAGCAG
    CCCTGGCTCA CAATCAAGGA ACCAATTACT CCAATCACCC GTGAGGCAGG GGGCGGGACA
    GACGAGGTGG AGGAGCTGAT TCGCCGGCAT GAAGCATTCC GGAAAGCTGC GTCCACTTGG
    AAAGACCATT TCAGCTCTTT ACGACAGGCA GAAAAGATGA AGGAGGAACT GAACAAACCA
    CCTTCTACCT CCTCTCTTCT CAGCAGGCAG ATGTTCCCTC TCTCCTGTCT TGTGCCCAGC
    TCTTCTTCCT CCTCTTCATC ATCATCTCTT TTCCGTAACC CCCTTCAGGA CTCACTCCTC
    GAGTCTAAGC CTGGACCAGA CCACATGCAC TCCATAGAAC ACACAGTGAC TCACACTCTC
    CCTCAGCGGC TTGGGTCCAG CCTGAACCCC TACACACCTG TTATGAATGG CTCCTCCTAC
    CATGGGCTAC AGCAGCCATC GGGGGTCGTC AATGAAAGTC CTTTATATCA ATCGATAAAA
    CAACAGGGTG TTTCAAGTTT GGATAATTCC AGCTATCAGG GGCTTAACCA ACAAGTTGGT
    GTCTTTGGGG GGAACAGCTC CAGCTATACT GGACTAAATC AACAGGGTGC TTTAGCAGAG
    GACGGCTCCA GTTACCATAG CCTGAACCAT TTAGGTGCCG TAGGGGTTGT GGAACCCAAA
    ATTGGATATG CTTGGCAGCA TCTGAAAGCT GACTGCTTTC AGCCCAAAAT CAACCACATT
    CACAAAGCGG TTTCACCTTT ACTGGAGGCC CACCGAGCTC AGCTTGCTAG CGCAGGAGCA
    AACATGCCGG CTCCTCCAGT GGGCATGGAC CCCTCGCTGG AGATGATCCA CAGTCGTTTA
    CAAAGAGACC CCCGGGGGAG CCGCTCTGAC CCACAGATGG ATCACCTGCG GCGGGAGAGG
    GAGTACAGGC TAGGCAGACA GACTTCCAGC GAGCAGGAGA TCCAGGCTCG ACTCAACGAG
    CTGCCGTTGA TTGTTCGGCA GGAGCGCTAC CGTCGAAGAA TGGAGAGACA GTCGTCCAGT
    GAGCAGGAGG CCAGCGGAAA GCAGAGAGTG CAAAAACATG ACTCCAGTGA TGCAGAATCT
    GGGAAAGAGC CATCTGACAA GAAGCCATCT GGGGAGAAGC GTTCCACTAT GGCTGAGATT
    GTTGAGCAAG TGCAGGAGAG AGAAGCTTGC CATGCGAGAG GAGAGGTTTA CAGACCCGCC
    AGTAGCCTCT CTGCACCAGT CAGTCGTCTA GATCGTCCTC GAGCACGTGA CCGTCCCAAA
    CCACGAAGAA GGCCTCGTCC AAAAGAGCCA GAAGAGCCAC GGAGGTCACG CTCTGCACCT
    GCACAGAGTG TCCCTTCTAC ACCTCAACCT CCGACTCACA CCGTCCACCA TGAGGGCTTC
    CTGTACAGGA AACACGAAGA GGAGGGGAAA GAGAGAAGCC CAAACAGTAA ATCGTGGGTA
    AATCTATTCT GCGTGCTCAA ACAAGGGGAG ATCGGTTTTT ACAAGGATGC ACGACACACA
    ACCACACCCT ACAATGACGA GCCTCTTCTT AACCTTGCCA TCTGCGAATT TGACACCACC
    AATGGATACA AAAAGAAGAA GAATGTCTTC ATTCTCAGGA CCACTGCTGA AGGGGACTAC
    ATCTTCCTGG CTAAAGATGA GGAGGATCTG AAAGGTTGGG TCAACAGCAT CAACGCTAGC
    CTGAAAGAGA TCGAGGACAT TGCCAAGTAC GAGAAAGCCA CAAACTCCTC CACCGACCCC
    GACAAATCAG AGCGCAAGGA GCGTTCTGAA GGGGCGGAGC CATCAGAGGG GGTGGAGAGA
    TCAGAGAGGT CAGAGAAGTC TGAGCGTTCA GACCGATCAG ATAAAATAGA GGCATCGGAC
    AAAAGCTCCG AAAGGAGGGA CGCATCTGAG AAGGAGAGAG GAGATAGAGG TGAACGAGGA
    GAGAGGGCGG AAAGAGGGGA TCGAGAGAGG GGTGAGCGAG GAGACCGGGG TTCCAGAGGA
    TCTAGCACTT CGGGAAAAAG CAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327260.1
    CDS11..3517
    Translation

    Target ORF information:

    RefSeq Version XM_021471585.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-27p18.5 (si:dkey-27p18.5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471585.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGATGC AGGTTCGGAA GGGGAGACTC GAGGAGGTTC TGGAAAGGGC AGAGGCTGTT 
    GCTGCCTTGC GGACCCCAGA GGTGGAGCTT GTGCGCGAGG GGGCGGGGCA TGTGAGACAG
    CTGTGGGAGG TGCTTCAGGT GGAGATGGAA CGCCGCACAG TGATGCTGGA TGCTGTGAAC
    CAAGCCCAGC AGTACTACAG CCAGGCAGCG AAGACAGAGT CCTGGCTCAG TGGACAGAAA
    CTTCAGGTCC TCAATGAGGA GAAAGGAAAC GACGAGGCCA GCACTCTTAG GTTACTGAAG
    GAGCAGTTGG CTTTAGAGCA AACAGTGGAG AATTATGCAG AGACAGTGGG CTCACTGTCC
    CAGCAGTGCC GTCGCATGCT TGAGTTGGGA CATCCAGACA GTGAGCAAAT CACCAAGCAG
    CAGGCTCATA TAGACCGGCT ATACGTATCT CTGAAAGACC TGGTAGAGCA GAGGAAAACA
    AAACTGGAGC AGCAGTACTG GTTATATCAG CTGAAGCGAG AGGTGGAGGC ACTGGAGAAA
    TGGATCTCTG AGAGGGAAGC TGTTGCCAGC TCCACTGAGC TCGGCCAGGA CCTGGAGCAT
    GTCACGGTTC TTCAGAGCAG CTTCACGCAG TTTTCCTCTG AGACACGTGC AGTGGGCCAG
    AAACAGATGG ATTCAGTCAA TAAGATGGTG AATGAGATGA TCGACTGCAG TCACTCTGAT
    GCTGCCACCA TTGCAGAGTG GAAAGACGGA CTGAACGAGT CCTGGGCGGA TCTACTGGAG
    CTCATGGAGA CCAGAGCGCA GATGTTGGCA GCATCCTACC AGCTGCACAA GTTCTTCACT
    GACTGTCAGG AGGTATTGGT ACAGATAGAA GGAAAGATGA GACAGCTGCC AGAGGTGAGG
    TCCTGCCAGG TGAGCTCGGC CAACCCTGGC ACACTACAGA GACTCCTGCA CTCTTTCGAA
    CACTCTCTGC AGCTGCTGGT CTCGCAGGTA CGCCAACTGC AAGAAAATGC AGTTCAGCTA
    CGTGCAATCT ATGCTGGGGA GAAGGCAGAT GCCATTTTGA GCCGTGAGCA GGAAGTGATG
    CAGGCCTGGA AAGAGCTCCT CGTTGCATGT GAGGGCAGCC GTGTGCAGGT CACCACTGTA
    ACCGATAAGA TCCAGTTCTT TGCGGTGGTG CGGGAATTGA GCATGTGGAT GGATGGAATC
    ATGGGGCAGA TTGGCCCAAA TGACGATGCA AGGGACCTCT CAGTGCTAGA GGGCATGATG
    TCCCAACATC AGAATCTGAA GAGTAAGATG GAAAACAAGA GCAAGAACTT TGTGCACTGC
    GTGGAGATGG GAAAAATGCT GCTCGCTGCG CGCAACCCTG CAGCTGAAGA GGTGAAGGAA
    AAGCTGGAGT ATGTCATGGC AAAGCAGAAA GAGATGAATG AGCGATGGGA CCTGCACTGG
    GAGAAGCTTC AGCAAGCCAA GCAGAGGCTG CAGTCCACTC AGACGGGATT GACGGAGCAG
    CCCTGGCTCA CAATCAAGGA ACCAATTACT CCAATCACCC GTGAGGCAGG GGGCGGGACA
    GACGAGGTGG AGGAGCTGAT TCGCCGGCAT GAAGCATTCC GGAAAGCTGC GTCCACTTGG
    AAAGACCATT TCAGCTCTTT ACGACAGGCA GAAAAGATGA AGGAGGAACT GAACAAACCA
    CCTTCTACCT CCTCTCTTCT CAGCAGGCAG ATGTTCCCTC TCTCCTGTCT TGTGCCCAGC
    TCTTCTTCCT CCTCTTCATC ATCATCTCTT TTCCGTAACC CCCTTCAGGA CTCACTCCTC
    GAGTCTAAGC CTGGACCAGA CCACATGCAC TCCATAGAAC ACACAGTGAC TCACACTCTC
    CCTCAGCGGC TTGGGTCCAG CCTGAACCCC TACACACCTG TTATGAATGG CTCCTCCTAC
    CATGGGCTAC AGCAGCCATC GGGGGTCGTC AATGAAAGTC CTTTATATCA ATCGATAAAA
    CAACAGGGTG TTTCAAGTTT GGATAATTCC AGCTATCAGG GGCTTAACCA ACAAGTTGGT
    GTCTTTGGGG GGAACAGCTC CAGCTATACT GGACTAAATC AACAGGGTGC TTTAGCAGAG
    GACGGCTCCA GTTACCATAG CCTGAACCAT TTAGGTGCCG TAGGGGTTGT GGAACCCAAA
    ATTGGATATG CTTGGCAGCA TCTGAAAGCT GACTGCTTTC AGCCCAAAAT CAACCACATT
    CACAAAGCGG TTTCACCTTT ACTGGAGGCC CACCGAGCTC AGCTTGCTAG CGCAGGAGCA
    AACATGCCGG CTCCTCCAGT GGGCATGGAC CCCTCGCTGG AGATGATCCA CAGTCGTTTA
    CAAAGAGACC CCCGGGGGAG CCGCTCTGAC CCACAGATGG ATCACCTGCG GCGGGAGAGG
    GAGTACAGGC TAGGCAGACA GACTTCCAGC GAGCAGGAGA TCCAGGCTCG ACTCAACGAG
    CTGCCGTTGA TTGTTCGGCA GGAGCGCTAC CGTCGAAGAA TGGAGAGACA GTCGTCCAGT
    GAGCAGGAGG CCAGCGGAAA GCAGAGAGTG CAAAAACATG ACTCCAGTGA TGCAGAATCT
    GGGAAAGAGC CATCTGACAA GAAGCCATCT GGGGAGAAGC GTTCCACTAT GGCTGAGATT
    GTTGAGCAAG TGCAGGAGAG AGAAGCTTGC CATGCGAGAG GAGAGGTTTA CAGACCCGCC
    AGTAGCCTCT CTGCACCAGT CAGTCGTCTA GATCGTCCTC GAGCACGTGA CCGTCCCAAA
    CCACGAAGAA GGCCTCGTCC AAAAGAGCCA GAAGAGCCAC GGAGGTCACG CTCTGCACCT
    GCACAGAGTG TCCCTTCTAC ACCTCAACCT CCGACTCACA CCGTCCACCA TGAGGGCTTC
    CTGTACAGGA AACACGAAGA GGAGGGGAAA GAGAGAAGCC CAAACAGTAA ATCGTGGGTA
    AATCTATTCT GCGTGCTCAA ACAAGGGGAG ATCGGTTTTT ACAAGGATGC ACGACACACA
    ACCACACCCT ACAATGACGA GCCTCTTCTT AACCTTGCCA TCTGCGAATT TGACACCACC
    AATGGATACA AAAAGAAGAA GAATGTCTTC ATTCTCAGGA CCACTGCTGA AGGGGACTAC
    ATCTTCCTGG CTAAAGATGA GGAGGATCTG AAAGGTTGGG TCAACAGCAT CAACGCTAGC
    CTGAAAGAGA TCGAGGACAT TGCCAAGTAC GAGAAAGCCA CAAACTCCTC CACCGACCCC
    GACAAATCAG AGCGCAAGGA GCGTTCTGAA GGGGCGGAGC CATCAGAGGG GGTGGAGAGA
    TCAGAGAGGT CAGAGAAGTC TGAGCGTTCA GACCGATCAG ATAAAATAGA GGCATCGGAC
    AAAAGCTCCG AAAGGAGGGA CGCATCTGAG AAGGAGAGAG GAGATAGAGG TGAACGAGGA
    GAGAGGGCGG AAAGAGGGGA TCGAGAGAGG GGTGAGCGAG GAGACCGGGG TTCCAGAGGA
    TCTAGCACTT CGGGAAAAAG CAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10392
    Clone ID Related Accession (Same CDS sequence) XM_001342718.6 , XM_001342718.5
    Accession Version XM_001342718.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin beta chain, non-erythrocytic 4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001342718.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTGACAG CTCGCGATGG AGCTAGAGAT GAAGCTCAGA AGCTGCACAG GAAATGGATG 
    AAGCACCAGG CCTTCATGGC CGAACTGGCC CGCAACAAAG AATGGCTCGC CAAGATTGAA
    CAGGAGGGCG AAGAGCTGAT CCAGGAGCAG CCCGAGTTGC GATCGGTGGT GGAAATGAAG
    TTGAAGGAGA TCAGAGAGTG CTGGTCTCAC CTGGAGAACA CCACTAAAGT CAAGGCCCGG
    CAGCTCTTTG AAACCCAGAA CCACCGGACT ACTGACCTGC CCACTAACAA CCTCAGCGAC
    CTGGACCAGC ATTTGAACAT CATACAGGAG CAGCCGCCCA CACTGGGCTC CACCCACACC
    ACACAACCCA CCCTTCTCCA GCCCCGTCCC ACTTTTAGCC AGCAGCTCCA GAGGATACAA
    TCATGGGAAG ACCAAATGCA GCTTTTTCAT GGCGTTAGTG AAGTCATGGC TGGTGCTGAA
    CACAGGAGAC CTGAAGGAGA AGAGCAAGGA GGCGTGCCAG AAACCCGGAT TGTGCGTCTG
    ATTGAGCCTC TGAAGGAAAG GAGGCGGATT CTTCTTGCCT CAAAAGAATT GCATCAAGTC
    ACACAGGACC TGGAGGATGA GATTATATGG ATTCAGGAGC GGTTGCATTT GGCCTCATCT
    ATAGAATATG GGACCAATTT GCAGAGTGTC CAAAATTTAA TGAAAAACCA TGAGGCACTG
    CAGATGGAGA TGCAGGTTCG GAAGGGGAGA CTCGAGGAGG TTCTGGAAAG GGCAGAGGCT
    GTTGCTGCCT TGCGGACCCC AGAGGTGGAG CTTGTGCGCG AGGGGGCGGG GCATGTGAGA
    CAGCTGTGGG AGGTGCTTCA GGTGGAGATG GAACGCCGCA CAGTGATGCT GGATGCTGTG
    AACCAAGCCC AGCAGTACTA CAGCCAGGCA GCGAAGACAG AGTCCTGGCT CAGTGGACAG
    AAACTTCAGG TCCTCAATGA GGAGAAAGGA AACGACGAGG CCAGCACTCT TAGGTTACTG
    AAGGAGCAGT TGGCTTTAGA GCAAACAGTG GAGAATTATG CAGAGACAGT GGGCTCACTG
    TCCCAGCAGT GCCGTCGCAT GCTTGAGTTG GGACATCCAG ACAGTGAGCA AATCACCAAG
    CAGCAGGCTC ATATAGACCG GCTATACGTA TCTCTGAAAG ACCTGGTAGA GCAGAGGAAA
    ACAAAACTGG AGCAGCAGTA CTGGTTATAT CAGCTGAAGC GAGAGGTGGA GGCACTGGAG
    AAATGGATCT CTGAGAGGGA AGCTGTTGCC AGCTCCACTG AGCTCGGCCA GGACCTGGAG
    CATGTCACGG TTCTTCAGAG CAGCTTCACG CAGTTTTCCT CTGAGACACG TGCAGTGGGC
    CAGAAACAGA TGGATTCAGT CAATAAGATG GTGAATGAGA TGATCGACTG CAGTCACTCT
    GATGCTGCCA CCATTGCAGA GTGGAAAGAC GGACTGAACG AGTCCTGGGC GGATCTACTG
    GAGCTCATGG AGACCAGAGC GCAGATGTTG GCAGCATCCT ACCAGCTGCA CAAGTTCTTC
    ACTGACTGTC AGGAGGTATT GGTACAGATA GAAGGAAAGA TGAGACAGCT GCCAGAGGTG
    AGGTCCTGCC AGGTGAGCTC GGCCAACCCT GGCACACTAC AGAGACTCCT GCACTCTTTC
    GAACACTCTC TGCAGCTGCT GGTCTCGCAG GTACGCCAAC TGCAAGAAAA TGCAGTTCAG
    CTACGTGCAA TCTATGCTGG GGAGAAGGCA GATGCCATTT TGAGCCGTGA GCAGGAAGTG
    ATGCAGGCCT GGAAAGAGCT CCTCGTTGCA TGTGAGGGCA GCCGTGTGCA GGTCACCACT
    GTAACCGATA AGATCCAGTT CTTTGCGGTG GTGCGGGAAT TGAGCATGTG GATGGATGGA
    ATCATGGGGC AGATTGGCCC AAATGACGAT GCAAGGGACC TCTCAGTGCT AGAGGGCATG
    ATGTCCCAAC ATCAGAATCT GAAGAGTAAG ATGGAAAACA AGAGCAAGAA CTTTGTGCAC
    TGCGTGGAGA TGGGAAAAAT GCTGCTCGCT GCGCGCAACC CTGCAGCTGA AGAGGTGAAG
    GAAAAGCTGG AGTATGTCAT GGCAAAGCAG AAAGAGATGA ATGAGCGATG GGACCTGCAC
    TGGGAGAAGC TTCAGCAAGC CAAGCAGAGG CTGCAGTCCA CTCAGACGGG ATTGACGGAG
    CAGCCCTGGC TCACAATCAA GGAACCAATT ACTCCAATCA CCCGTGAGGC AGGGGGCGGG
    ACAGACGAGG TGGAGGAGCT GATTCGCCGG CATGAAGCAT TCCGGAAAGC TGCGTCCACT
    TGGAAAGACC ATTTCAGCTC TTTACGACAG GCAGAAAAGA TGAAGGAGGA ACTGAACAAA
    CCACCTTCTA CCTCCTCTCT TCTCAGCAGG CAGATGTTCC CTCTCTCCTG TCTTGTGCCC
    AGCTCTTCTT CCTCCTCTTC ATCATCATCT CTTTTCCGTA ACCCCCTTCA GGACTCACTC
    CTCGAGTCTA AGCCTGGACC AGACCACATG CACTCCATAG AACACACAGT GACTCACACT
    CTCCCTCAGC GGCTTGGGTC CAGCCTGAAC CCCTACACAC CTGTTATGAA TGGCTCCTCC
    TACCATGGGC TACAGCAGCC ATCGGGGGTC GTCAATGAAA GTCCTTTATA TCAATCGATA
    AAACAACAGG GTGTTTCAAG TTTGGATAAT TCCAGCTATC AGGGGCTTAA CCAACAAGTT
    GGTGTCTTTG GGGGGAACAG CTCCAGCTAT ACTGGACTAA ATCAACAGGG TGCTTTAGCA
    GAGGACGGCT CCAGTTACCA TAGCCTGAAC CATTTAGGTG CCGTAGGGGT TGTGGAACCC
    AAAATTGGAT ATGCTTGGCA GCATCTGAAA GCTGACTGCT TTCAGCCCAA AATCAACCAC
    ATTCACAAAG CGGTTTCACC TTTACTGGAG GCCCACCGAG CTCAGCTTGC TAGCGCAGGA
    GCAAACATGC CGGCTCCTCC AGTGGGCATG GACCCCTCGC TGGAGATGAT CCACAGTCGT
    TTACAAAGAG ACCCCCGGGG GAGCCGCTCT GACCCACAGA TGGATCACCT GCGGCGGGAG
    AGGGAGTACA GGCTAGGCAG ACAGACTTCC AGCGAGCAGG AGATCCAGGC TCGACTCAAC
    GAGCTGCCGT TGATTGTTCG GCAGGAGCGC TACCGTCGAA GAATGGAGAG ACAGTCGTCC
    AGTGAGCAGG AGGCCAGCGG AAAGCAGAGA GTGCAAAAAC ATGACTCCAG TGATGCAGAA
    TCTGGGAAAG AGCCATCTGA CAAGAAGCCA TCTGGGGAGA AGCGTTCCAC TATGGCTGAG
    ATTGTTGAGC AAGTGCAGGA GAGAGAAGCT TGCCATGCGA GAGGAGAGGT TTACAGACCC
    GCCAGTAGCC TCTCTGCACC AGTCAGTCGT CTAGATCGTC CTCGAGCACG TGACCGTCCC
    AAACCACGAA GAAGGCCTCG TCCAAAAGAG CCAGAAGAGC CACGGAGGTC ACGCTCTGCA
    CCTGCACAGA GTGTCCCTTC TACACCTCAA CCTCCGACTC ACACCGTCCA CCATGAGGGC
    TTCCTGTACA GGAAACACGA AGAGGAGGGG AAAGAGAGAA GCCCAAACAG TAAATCGTGG
    GTAAATCTAT TCTGCGTGCT CAAACAAGGG GAGATCGGTT TTTACAAGGA TGCACGACAC
    ACAACCACAC CCTACAATGA CGAGCCTCTT CTTAACCTTG CCATCTGCGA ATTTGACACC
    ACCAATGGAT ACAAAAAGAA GAAGAATGTC TTCATTCTCA GGACCACTGC TGAAGGGGAC
    TACATCTTCC TGGCTAAAGA TGAGGAGGAT CTGAAAGGTT GGGTCAACAG CATCAACGCT
    AGCCTGAAAG AGATCGAGGA CATTGCCAAG TACGAGAAAG CCACAAACTC CTCCACCGAC
    CCCGACAAAT CAGAGCGCAA GGAGCGTTCT GAAGGGGCGG AGCCATCAGA GGGGGTGGAG
    AGATCAGAGA GGTCAGAGAA GTCTGAGCGT TCAGACCGAT CAGATAAAAT AGAGGCATCG
    GACAAAAGCT CCGAAAGGAG GGACGCATCT GAGAAGGAGA GAGGAGATAG AGGTGAACGA
    GGAGAGAGGG CGGAAAGAGG GGATCGAGAG AGGGGTGAGC GAGGAGACCG GGGTTCCAGA
    GGATCTAGCA CTTCGGGAAA AAGCAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001342754.3
    CDS645..4874
    Translation

    Target ORF information:

    RefSeq Version XM_001342718.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-27p18.5 (si:dkey-27p18.5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001342718.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTGACAG CTCGCGATGG AGCTAGAGAT GAAGCTCAGA AGCTGCACAG GAAATGGATG 
    AAGCACCAGG CCTTCATGGC CGAACTGGCC CGCAACAAAG AATGGCTCGC CAAGATTGAA
    CAGGAGGGCG AAGAGCTGAT CCAGGAGCAG CCCGAGTTGC GATCGGTGGT GGAAATGAAG
    TTGAAGGAGA TCAGAGAGTG CTGGTCTCAC CTGGAGAACA CCACTAAAGT CAAGGCCCGG
    CAGCTCTTTG AAACCCAGAA CCACCGGACT ACTGACCTGC CCACTAACAA CCTCAGCGAC
    CTGGACCAGC ATTTGAACAT CATACAGGAG CAGCCGCCCA CACTGGGCTC CACCCACACC
    ACACAACCCA CCCTTCTCCA GCCCCGTCCC ACTTTTAGCC AGCAGCTCCA GAGGATACAA
    TCATGGGAAG ACCAAATGCA GCTTTTTCAT GGCGTTAGTG AAGTCATGGC TGGTGCTGAA
    CACAGGAGAC CTGAAGGAGA AGAGCAAGGA GGCGTGCCAG AAACCCGGAT TGTGCGTCTG
    ATTGAGCCTC TGAAGGAAAG GAGGCGGATT CTTCTTGCCT CAAAAGAATT GCATCAAGTC
    ACACAGGACC TGGAGGATGA GATTATATGG ATTCAGGAGC GGTTGCATTT GGCCTCATCT
    ATAGAATATG GGACCAATTT GCAGAGTGTC CAAAATTTAA TGAAAAACCA TGAGGCACTG
    CAGATGGAGA TGCAGGTTCG GAAGGGGAGA CTCGAGGAGG TTCTGGAAAG GGCAGAGGCT
    GTTGCTGCCT TGCGGACCCC AGAGGTGGAG CTTGTGCGCG AGGGGGCGGG GCATGTGAGA
    CAGCTGTGGG AGGTGCTTCA GGTGGAGATG GAACGCCGCA CAGTGATGCT GGATGCTGTG
    AACCAAGCCC AGCAGTACTA CAGCCAGGCA GCGAAGACAG AGTCCTGGCT CAGTGGACAG
    AAACTTCAGG TCCTCAATGA GGAGAAAGGA AACGACGAGG CCAGCACTCT TAGGTTACTG
    AAGGAGCAGT TGGCTTTAGA GCAAACAGTG GAGAATTATG CAGAGACAGT GGGCTCACTG
    TCCCAGCAGT GCCGTCGCAT GCTTGAGTTG GGACATCCAG ACAGTGAGCA AATCACCAAG
    CAGCAGGCTC ATATAGACCG GCTATACGTA TCTCTGAAAG ACCTGGTAGA GCAGAGGAAA
    ACAAAACTGG AGCAGCAGTA CTGGTTATAT CAGCTGAAGC GAGAGGTGGA GGCACTGGAG
    AAATGGATCT CTGAGAGGGA AGCTGTTGCC AGCTCCACTG AGCTCGGCCA GGACCTGGAG
    CATGTCACGG TTCTTCAGAG CAGCTTCACG CAGTTTTCCT CTGAGACACG TGCAGTGGGC
    CAGAAACAGA TGGATTCAGT CAATAAGATG GTGAATGAGA TGATCGACTG CAGTCACTCT
    GATGCTGCCA CCATTGCAGA GTGGAAAGAC GGACTGAACG AGTCCTGGGC GGATCTACTG
    GAGCTCATGG AGACCAGAGC GCAGATGTTG GCAGCATCCT ACCAGCTGCA CAAGTTCTTC
    ACTGACTGTC AGGAGGTATT GGTACAGATA GAAGGAAAGA TGAGACAGCT GCCAGAGGTG
    AGGTCCTGCC AGGTGAGCTC GGCCAACCCT GGCACACTAC AGAGACTCCT GCACTCTTTC
    GAACACTCTC TGCAGCTGCT GGTCTCGCAG GTACGCCAAC TGCAAGAAAA TGCAGTTCAG
    CTACGTGCAA TCTATGCTGG GGAGAAGGCA GATGCCATTT TGAGCCGTGA GCAGGAAGTG
    ATGCAGGCCT GGAAAGAGCT CCTCGTTGCA TGTGAGGGCA GCCGTGTGCA GGTCACCACT
    GTAACCGATA AGATCCAGTT CTTTGCGGTG GTGCGGGAAT TGAGCATGTG GATGGATGGA
    ATCATGGGGC AGATTGGCCC AAATGACGAT GCAAGGGACC TCTCAGTGCT AGAGGGCATG
    ATGTCCCAAC ATCAGAATCT GAAGAGTAAG ATGGAAAACA AGAGCAAGAA CTTTGTGCAC
    TGCGTGGAGA TGGGAAAAAT GCTGCTCGCT GCGCGCAACC CTGCAGCTGA AGAGGTGAAG
    GAAAAGCTGG AGTATGTCAT GGCAAAGCAG AAAGAGATGA ATGAGCGATG GGACCTGCAC
    TGGGAGAAGC TTCAGCAAGC CAAGCAGAGG CTGCAGTCCA CTCAGACGGG ATTGACGGAG
    CAGCCCTGGC TCACAATCAA GGAACCAATT ACTCCAATCA CCCGTGAGGC AGGGGGCGGG
    ACAGACGAGG TGGAGGAGCT GATTCGCCGG CATGAAGCAT TCCGGAAAGC TGCGTCCACT
    TGGAAAGACC ATTTCAGCTC TTTACGACAG GCAGAAAAGA TGAAGGAGGA ACTGAACAAA
    CCACCTTCTA CCTCCTCTCT TCTCAGCAGG CAGATGTTCC CTCTCTCCTG TCTTGTGCCC
    AGCTCTTCTT CCTCCTCTTC ATCATCATCT CTTTTCCGTA ACCCCCTTCA GGACTCACTC
    CTCGAGTCTA AGCCTGGACC AGACCACATG CACTCCATAG AACACACAGT GACTCACACT
    CTCCCTCAGC GGCTTGGGTC CAGCCTGAAC CCCTACACAC CTGTTATGAA TGGCTCCTCC
    TACCATGGGC TACAGCAGCC ATCGGGGGTC GTCAATGAAA GTCCTTTATA TCAATCGATA
    AAACAACAGG GTGTTTCAAG TTTGGATAAT TCCAGCTATC AGGGGCTTAA CCAACAAGTT
    GGTGTCTTTG GGGGGAACAG CTCCAGCTAT ACTGGACTAA ATCAACAGGG TGCTTTAGCA
    GAGGACGGCT CCAGTTACCA TAGCCTGAAC CATTTAGGTG CCGTAGGGGT TGTGGAACCC
    AAAATTGGAT ATGCTTGGCA GCATCTGAAA GCTGACTGCT TTCAGCCCAA AATCAACCAC
    ATTCACAAAG CGGTTTCACC TTTACTGGAG GCCCACCGAG CTCAGCTTGC TAGCGCAGGA
    GCAAACATGC CGGCTCCTCC AGTGGGCATG GACCCCTCGC TGGAGATGAT CCACAGTCGT
    TTACAAAGAG ACCCCCGGGG GAGCCGCTCT GACCCACAGA TGGATCACCT GCGGCGGGAG
    AGGGAGTACA GGCTAGGCAG ACAGACTTCC AGCGAGCAGG AGATCCAGGC TCGACTCAAC
    GAGCTGCCGT TGATTGTTCG GCAGGAGCGC TACCGTCGAA GAATGGAGAG ACAGTCGTCC
    AGTGAGCAGG AGGCCAGCGG AAAGCAGAGA GTGCAAAAAC ATGACTCCAG TGATGCAGAA
    TCTGGGAAAG AGCCATCTGA CAAGAAGCCA TCTGGGGAGA AGCGTTCCAC TATGGCTGAG
    ATTGTTGAGC AAGTGCAGGA GAGAGAAGCT TGCCATGCGA GAGGAGAGGT TTACAGACCC
    GCCAGTAGCC TCTCTGCACC AGTCAGTCGT CTAGATCGTC CTCGAGCACG TGACCGTCCC
    AAACCACGAA GAAGGCCTCG TCCAAAAGAG CCAGAAGAGC CACGGAGGTC ACGCTCTGCA
    CCTGCACAGA GTGTCCCTTC TACACCTCAA CCTCCGACTC ACACCGTCCA CCATGAGGGC
    TTCCTGTACA GGAAACACGA AGAGGAGGGG AAAGAGAGAA GCCCAAACAG TAAATCGTGG
    GTAAATCTAT TCTGCGTGCT CAAACAAGGG GAGATCGGTT TTTACAAGGA TGCACGACAC
    ACAACCACAC CCTACAATGA CGAGCCTCTT CTTAACCTTG CCATCTGCGA ATTTGACACC
    ACCAATGGAT ACAAAAAGAA GAAGAATGTC TTCATTCTCA GGACCACTGC TGAAGGGGAC
    TACATCTTCC TGGCTAAAGA TGAGGAGGAT CTGAAAGGTT GGGTCAACAG CATCAACGCT
    AGCCTGAAAG AGATCGAGGA CATTGCCAAG TACGAGAAAG CCACAAACTC CTCCACCGAC
    CCCGACAAAT CAGAGCGCAA GGAGCGTTCT GAAGGGGCGG AGCCATCAGA GGGGGTGGAG
    AGATCAGAGA GGTCAGAGAA GTCTGAGCGT TCAGACCGAT CAGATAAAAT AGAGGCATCG
    GACAAAAGCT CCGAAAGGAG GGACGCATCT GAGAAGGAGA GAGGAGATAG AGGTGAACGA
    GGAGAGAGGG CGGAAAGAGG GGATCGAGAG AGGGGTGAGC GAGGAGACCG GGGTTCCAGA
    GGATCTAGCA CTTCGGGAAA AAGCAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10392
    Clone ID Related Accession (Same CDS sequence) XM_001342718.6 , XM_001342718.5
    Accession Version XM_001342718.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin beta chain, non-erythrocytic 4-like
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_001342718.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTGACAG CTCGCGATGG AGCTAGAGAT GAAGCTCAGA AGCTGCACAG GAAATGGATG 
    AAGCACCAGG CCTTCATGGC CGAACTGGCC CGCAACAAAG AATGGCTCGC CAAGATTGAA
    CAGGAGGGCG AAGAGCTGAT CCAGGAGCAG CCCGAGTTGC GATCGGTGGT GGAAATGAAG
    TTGAAGGAGA TCAGAGAGTG CTGGTCTCAC CTGGAGAACA CCACTAAAGT CAAGGCCCGG
    CAGCTCTTTG AAACCCAGAA CCACCGGACT ACTGACCTGC CCACTAACAA CCTCAGCGAC
    CTGGACCAGC ATTTGAACAT CATACAGGAG CAGCCGCCCA CACTGGGCTC CACCCACACC
    ACACAACCCA CCCTTCTCCA GCCCCGTCCC ACTTTTAGCC AGCAGCTCCA GAGGATACAA
    TCATGGGAAG ACCAAATGCA GCTTTTTCAT GGCGTTAGTG AAGTCATGGC TGGTGCTGAA
    CACAGGAGAC CTGAAGGAGA AGAGCAAGGA GGCGTGCCAG AAACCCGGAT TGTGCGTCTG
    ATTGAGCCTC TGAAGGAAAG GAGGCGGATT CTTCTTGCCT CAAAAGAATT GCATCAAGTC
    ACACAGGACC TGGAGGATGA GATTATATGG ATTCAGGAGC GGTTGCATTT GGCCTCATCT
    ATAGAATATG GGACCAATTT GCAGAGTGTC CAAAATTTAA TGAAAAACCA TGAGGCACTG
    CAGATGGAGA TGCAGGTTCG GAAGGGGAGA CTCGAGGAGG TTCTGGAAAG GGCAGAGGCT
    GTTGCTGCCT TGCGGACCCC AGAGGTGGAG CTTGTGCGCG AGGGGGCGGG GCATGTGAGA
    CAGCTGTGGG AGGTGCTTCA GGTGGAGATG GAACGCCGCA CAGTGATGCT GGATGCTGTG
    AACCAAGCCC AGCAGTACTA CAGCCAGGCA GCGAAGACAG AGTCCTGGCT CAGTGGACAG
    AAACTTCAGG TCCTCAATGA GGAGAAAGGA AACGACGAGG CCAGCACTCT TAGGTTACTG
    AAGGAGCAGT TGGCTTTAGA GCAAACAGTG GAGAATTATG CAGAGACAGT GGGCTCACTG
    TCCCAGCAGT GCCGTCGCAT GCTTGAGTTG GGACATCCAG ACAGTGAGCA AATCACCAAG
    CAGCAGGCTC ATATAGACCG GCTATACGTA TCTCTGAAAG ACCTGGTAGA GCAGAGGAAA
    ACAAAACTGG AGCAGCAGTA CTGGTTATAT CAGCTGAAGC GAGAGGTGGA GGCACTGGAG
    AAATGGATCT CTGAGAGGGA AGCTGTTGCC AGCTCCACTG AGCTCGGCCA GGACCTGGAG
    CATGTCACGG TTCTTCAGAG CAGCTTCACG CAGTTTTCCT CTGAGACACG TGCAGTGGGC
    CAGAAACAGA TGGATTCAGT CAATAAGATG GTGAATGAGA TGATCGACTG CAGTCACTCT
    GATGCTGCCA CCATTGCAGA GTGGAAAGAC GGACTGAACG AGTCCTGGGC GGATCTACTG
    GAGCTCATGG AGACCAGAGC GCAGATGTTG GCAGCATCCT ACCAGCTGCA CAAGTTCTTC
    ACTGACTGTC AGGAGGTATT GGTACAGATA GAAGGAAAGA TGAGACAGCT GCCAGAGGTG
    AGGTCCTGCC AGGTGAGCTC GGCCAACCCT GGCACACTAC AGAGACTCCT GCACTCTTTC
    GAACACTCTC TGCAGCTGCT GGTCTCGCAG GTACGCCAAC TGCAAGAAAA TGCAGTTCAG
    CTACGTGCAA TCTATGCTGG GGAGAAGGCA GATGCCATTT TGAGCCGTGA GCAGGAAGTG
    ATGCAGGCCT GGAAAGAGCT CCTCGTTGCA TGTGAGGGCA GCCGTGTGCA GGTCACCACT
    GTAACCGATA AGATCCAGTT CTTTGCGGTG GTGCGGGAAT TGAGCATGTG GATGGATGGA
    ATCATGGGGC AGATTGGCCC AAATGACGAT GCAAGGGACC TCTCAGTGCT AGAGGGCATG
    ATGTCCCAAC ATCAGAATCT GAAGAGTAAG ATGGAAAACA AGAGCAAGAA CTTTGTGCAC
    TGCGTGGAGA TGGGAAAAAT GCTGCTCGCT GCGCGCAACC CTGCAGCTGA AGAGGTGAAG
    GAAAAGCTGG AGTATGTCAT GGCAAAGCAG AAAGAGATGA ATGAGCGATG GGACCTGCAC
    TGGGAGAAGC TTCAGCAAGC CAAGCAGAGG CTGCAGTCCA CTCAGACGGG ATTGACGGAG
    CAGCCCTGGC TCACAATCAA GGAACCAATT ACTCCAATCA CCCGTGAGGC AGGGGGCGGG
    ACAGACGAGG TGGAGGAGCT GATTCGCCGG CATGAAGCAT TCCGGAAAGC TGCGTCCACT
    TGGAAAGACC ATTTCAGCTC TTTACGACAG GCAGAAAAGA TGAAGGAGGA ACTGAACAAA
    CCACCTTCTA CCTCCTCTCT TCTCAGCAGG CAGATGTTCC CTCTCTCCTG TCTTGTGCCC
    AGCTCTTCTT CCTCCTCTTC ATCATCATCT CTTTTCCGTA ACCCCCTTCA GGACTCACTC
    CTCGAGTCTA AGCCTGGACC AGACCACATG CACTCCATAG AACACACAGT GACTCACACT
    CTCCCTCAGC GGCTTGGGTC CAGCCTGAAC CCCTACACAC CTGTTATGAA TGGCTCCTCC
    TACCATGGGC TACAGCAGCC ATCGGGGGTC GTCAATGAAA GTCCTTTATA TCAATCGATA
    AAACAACAGG GTGTTTCAAG TTTGGATAAT TCCAGCTATC AGGGGCTTAA CCAACAAGTT
    GGTGTCTTTG GGGGGAACAG CTCCAGCTAT ACTGGACTAA ATCAACAGGG TGCTTTAGCA
    GAGGACGGCT CCAGTTACCA TAGCCTGAAC CATTTAGGTG CCGTAGGGGT TGTGGAACCC
    AAAATTGGAT ATGCTTGGCA GCATCTGAAA GCTGACTGCT TTCAGCCCAA AATCAACCAC
    ATTCACAAAG CGGTTTCACC TTTACTGGAG GCCCACCGAG CTCAGCTTGC TAGCGCAGGA
    GCAAACATGC CGGCTCCTCC AGTGGGCATG GACCCCTCGC TGGAGATGAT CCACAGTCGT
    TTACAAAGAG ACCCCCGGGG GAGCCGCTCT GACCCACAGA TGGATCACCT GCGGCGGGAG
    AGGGAGTACA GGCTAGGCAG ACAGACTTCC AGCGAGCAGG AGATCCAGGC TCGACTCAAC
    GAGCTGCCGT TGATTGTTCG GCAGGAGCGC TACCGTCGAA GAATGGAGAG ACAGTCGTCC
    AGTGAGCAGG AGGCCAGCGG AAAGCAGAGA GTGCAAAAAC ATGACTCCAG TGATGCAGAA
    TCTGGGAAAG AGCCATCTGA CAAGAAGCCA TCTGGGGAGA AGCGTTCCAC TATGGCTGAG
    ATTGTTGAGC AAGTGCAGGA GAGAGAAGCT TGCCATGCGA GAGGAGAGGT TTACAGACCC
    GCCAGTAGCC TCTCTGCACC AGTCAGTCGT CTAGATCGTC CTCGAGCACG TGACCGTCCC
    AAACCACGAA GAAGGCCTCG TCCAAAAGAG CCAGAAGAGC CACGGAGGTC ACGCTCTGCA
    CCTGCACAGA GTGTCCCTTC TACACCTCAA CCTCCGACTC ACACCGTCCA CCATGAGGGC
    TTCCTGTACA GGAAACACGA AGAGGAGGGG AAAGAGAGAA GCCCAAACAG TAAATCGTGG
    GTAAATCTAT TCTGCGTGCT CAAACAAGGG GAGATCGGTT TTTACAAGGA TGCACGACAC
    ACAACCACAC CCTACAATGA CGAGCCTCTT CTTAACCTTG CCATCTGCGA ATTTGACACC
    ACCAATGGAT ACAAAAAGAA GAAGAATGTC TTCATTCTCA GGACCACTGC TGAAGGGGAC
    TACATCTTCC TGGCTAAAGA TGAGGAGGAT CTGAAAGGTT GGGTCAACAG CATCAACGCT
    AGCCTGAAAG AGATCGAGGA CATTGCCAAG TACGAGAAAG CCACAAACTC CTCCACCGAC
    CCCGACAAAT CAGAGCGCAA GGAGCGTTCT GAAGGGGCGG AGCCATCAGA GGGGGTGGAG
    AGATCAGAGA GGTCAGAGAA GTCTGAGCGT TCAGACCGAT CAGATAAAAT AGAGGCATCG
    GACAAAAGCT CCGAAAGGAG GGACGCATCT GAGAAGGAGA GAGGAGATAG AGGTGAACGA
    GGAGAGAGGG CGGAAAGAGG GGATCGAGAG AGGGGTGAGC GAGGAGACCG GGGTTCCAGA
    GGATCTAGCA CTTCGGGAAA AAGCAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001342754.3
    CDS610..4839
    Translation

    Target ORF information:

    RefSeq Version XM_001342718.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-27p18.5 (si:dkey-27p18.5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001342718.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCTGACAG CTCGCGATGG AGCTAGAGAT GAAGCTCAGA AGCTGCACAG GAAATGGATG 
    AAGCACCAGG CCTTCATGGC CGAACTGGCC CGCAACAAAG AATGGCTCGC CAAGATTGAA
    CAGGAGGGCG AAGAGCTGAT CCAGGAGCAG CCCGAGTTGC GATCGGTGGT GGAAATGAAG
    TTGAAGGAGA TCAGAGAGTG CTGGTCTCAC CTGGAGAACA CCACTAAAGT CAAGGCCCGG
    CAGCTCTTTG AAACCCAGAA CCACCGGACT ACTGACCTGC CCACTAACAA CCTCAGCGAC
    CTGGACCAGC ATTTGAACAT CATACAGGAG CAGCCGCCCA CACTGGGCTC CACCCACACC
    ACACAACCCA CCCTTCTCCA GCCCCGTCCC ACTTTTAGCC AGCAGCTCCA GAGGATACAA
    TCATGGGAAG ACCAAATGCA GCTTTTTCAT GGCGTTAGTG AAGTCATGGC TGGTGCTGAA
    CACAGGAGAC CTGAAGGAGA AGAGCAAGGA GGCGTGCCAG AAACCCGGAT TGTGCGTCTG
    ATTGAGCCTC TGAAGGAAAG GAGGCGGATT CTTCTTGCCT CAAAAGAATT GCATCAAGTC
    ACACAGGACC TGGAGGATGA GATTATATGG ATTCAGGAGC GGTTGCATTT GGCCTCATCT
    ATAGAATATG GGACCAATTT GCAGAGTGTC CAAAATTTAA TGAAAAACCA TGAGGCACTG
    CAGATGGAGA TGCAGGTTCG GAAGGGGAGA CTCGAGGAGG TTCTGGAAAG GGCAGAGGCT
    GTTGCTGCCT TGCGGACCCC AGAGGTGGAG CTTGTGCGCG AGGGGGCGGG GCATGTGAGA
    CAGCTGTGGG AGGTGCTTCA GGTGGAGATG GAACGCCGCA CAGTGATGCT GGATGCTGTG
    AACCAAGCCC AGCAGTACTA CAGCCAGGCA GCGAAGACAG AGTCCTGGCT CAGTGGACAG
    AAACTTCAGG TCCTCAATGA GGAGAAAGGA AACGACGAGG CCAGCACTCT TAGGTTACTG
    AAGGAGCAGT TGGCTTTAGA GCAAACAGTG GAGAATTATG CAGAGACAGT GGGCTCACTG
    TCCCAGCAGT GCCGTCGCAT GCTTGAGTTG GGACATCCAG ACAGTGAGCA AATCACCAAG
    CAGCAGGCTC ATATAGACCG GCTATACGTA TCTCTGAAAG ACCTGGTAGA GCAGAGGAAA
    ACAAAACTGG AGCAGCAGTA CTGGTTATAT CAGCTGAAGC GAGAGGTGGA GGCACTGGAG
    AAATGGATCT CTGAGAGGGA AGCTGTTGCC AGCTCCACTG AGCTCGGCCA GGACCTGGAG
    CATGTCACGG TTCTTCAGAG CAGCTTCACG CAGTTTTCCT CTGAGACACG TGCAGTGGGC
    CAGAAACAGA TGGATTCAGT CAATAAGATG GTGAATGAGA TGATCGACTG CAGTCACTCT
    GATGCTGCCA CCATTGCAGA GTGGAAAGAC GGACTGAACG AGTCCTGGGC GGATCTACTG
    GAGCTCATGG AGACCAGAGC GCAGATGTTG GCAGCATCCT ACCAGCTGCA CAAGTTCTTC
    ACTGACTGTC AGGAGGTATT GGTACAGATA GAAGGAAAGA TGAGACAGCT GCCAGAGGTG
    AGGTCCTGCC AGGTGAGCTC GGCCAACCCT GGCACACTAC AGAGACTCCT GCACTCTTTC
    GAACACTCTC TGCAGCTGCT GGTCTCGCAG GTACGCCAAC TGCAAGAAAA TGCAGTTCAG
    CTACGTGCAA TCTATGCTGG GGAGAAGGCA GATGCCATTT TGAGCCGTGA GCAGGAAGTG
    ATGCAGGCCT GGAAAGAGCT CCTCGTTGCA TGTGAGGGCA GCCGTGTGCA GGTCACCACT
    GTAACCGATA AGATCCAGTT CTTTGCGGTG GTGCGGGAAT TGAGCATGTG GATGGATGGA
    ATCATGGGGC AGATTGGCCC AAATGACGAT GCAAGGGACC TCTCAGTGCT AGAGGGCATG
    ATGTCCCAAC ATCAGAATCT GAAGAGTAAG ATGGAAAACA AGAGCAAGAA CTTTGTGCAC
    TGCGTGGAGA TGGGAAAAAT GCTGCTCGCT GCGCGCAACC CTGCAGCTGA AGAGGTGAAG
    GAAAAGCTGG AGTATGTCAT GGCAAAGCAG AAAGAGATGA ATGAGCGATG GGACCTGCAC
    TGGGAGAAGC TTCAGCAAGC CAAGCAGAGG CTGCAGTCCA CTCAGACGGG ATTGACGGAG
    CAGCCCTGGC TCACAATCAA GGAACCAATT ACTCCAATCA CCCGTGAGGC AGGGGGCGGG
    ACAGACGAGG TGGAGGAGCT GATTCGCCGG CATGAAGCAT TCCGGAAAGC TGCGTCCACT
    TGGAAAGACC ATTTCAGCTC TTTACGACAG GCAGAAAAGA TGAAGGAGGA ACTGAACAAA
    CCACCTTCTA CCTCCTCTCT TCTCAGCAGG CAGATGTTCC CTCTCTCCTG TCTTGTGCCC
    AGCTCTTCTT CCTCCTCTTC ATCATCATCT CTTTTCCGTA ACCCCCTTCA GGACTCACTC
    CTCGAGTCTA AGCCTGGACC AGACCACATG CACTCCATAG AACACACAGT GACTCACACT
    CTCCCTCAGC GGCTTGGGTC CAGCCTGAAC CCCTACACAC CTGTTATGAA TGGCTCCTCC
    TACCATGGGC TACAGCAGCC ATCGGGGGTC GTCAATGAAA GTCCTTTATA TCAATCGATA
    AAACAACAGG GTGTTTCAAG TTTGGATAAT TCCAGCTATC AGGGGCTTAA CCAACAAGTT
    GGTGTCTTTG GGGGGAACAG CTCCAGCTAT ACTGGACTAA ATCAACAGGG TGCTTTAGCA
    GAGGACGGCT CCAGTTACCA TAGCCTGAAC CATTTAGGTG CCGTAGGGGT TGTGGAACCC
    AAAATTGGAT ATGCTTGGCA GCATCTGAAA GCTGACTGCT TTCAGCCCAA AATCAACCAC
    ATTCACAAAG CGGTTTCACC TTTACTGGAG GCCCACCGAG CTCAGCTTGC TAGCGCAGGA
    GCAAACATGC CGGCTCCTCC AGTGGGCATG GACCCCTCGC TGGAGATGAT CCACAGTCGT
    TTACAAAGAG ACCCCCGGGG GAGCCGCTCT GACCCACAGA TGGATCACCT GCGGCGGGAG
    AGGGAGTACA GGCTAGGCAG ACAGACTTCC AGCGAGCAGG AGATCCAGGC TCGACTCAAC
    GAGCTGCCGT TGATTGTTCG GCAGGAGCGC TACCGTCGAA GAATGGAGAG ACAGTCGTCC
    AGTGAGCAGG AGGCCAGCGG AAAGCAGAGA GTGCAAAAAC ATGACTCCAG TGATGCAGAA
    TCTGGGAAAG AGCCATCTGA CAAGAAGCCA TCTGGGGAGA AGCGTTCCAC TATGGCTGAG
    ATTGTTGAGC AAGTGCAGGA GAGAGAAGCT TGCCATGCGA GAGGAGAGGT TTACAGACCC
    GCCAGTAGCC TCTCTGCACC AGTCAGTCGT CTAGATCGTC CTCGAGCACG TGACCGTCCC
    AAACCACGAA GAAGGCCTCG TCCAAAAGAG CCAGAAGAGC CACGGAGGTC ACGCTCTGCA
    CCTGCACAGA GTGTCCCTTC TACACCTCAA CCTCCGACTC ACACCGTCCA CCATGAGGGC
    TTCCTGTACA GGAAACACGA AGAGGAGGGG AAAGAGAGAA GCCCAAACAG TAAATCGTGG
    GTAAATCTAT TCTGCGTGCT CAAACAAGGG GAGATCGGTT TTTACAAGGA TGCACGACAC
    ACAACCACAC CCTACAATGA CGAGCCTCTT CTTAACCTTG CCATCTGCGA ATTTGACACC
    ACCAATGGAT ACAAAAAGAA GAAGAATGTC TTCATTCTCA GGACCACTGC TGAAGGGGAC
    TACATCTTCC TGGCTAAAGA TGAGGAGGAT CTGAAAGGTT GGGTCAACAG CATCAACGCT
    AGCCTGAAAG AGATCGAGGA CATTGCCAAG TACGAGAAAG CCACAAACTC CTCCACCGAC
    CCCGACAAAT CAGAGCGCAA GGAGCGTTCT GAAGGGGCGG AGCCATCAGA GGGGGTGGAG
    AGATCAGAGA GGTCAGAGAA GTCTGAGCGT TCAGACCGAT CAGATAAAAT AGAGGCATCG
    GACAAAAGCT CCGAAAGGAG GGACGCATCT GAGAAGGAGA GAGGAGATAG AGGTGAACGA
    GGAGAGAGGG CGGAAAGAGG GGATCGAGAG AGGGGTGAGC GAGGAGACCG GGGTTCCAGA
    GGATCTAGCA CTTCGGGAAA AAGCAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.