nphp1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nphp1
Entrez Gene ID	100002391
Full Name	nephronophthisis 1
Synonyms	im:7162391,wu:fi59g07,zgc:152930
General protein information	Preferred Names nephronophthisis 1 Names nephrocystin-1 nephrocystin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077170.1	NP_001070638.1	nephrocystin-1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NPHP1	XP_003309231.1
	NPHP1	XP_002799426.1
Danio rerio (zebrafish)	nphp1	NP_001070638.1
Bos taurus (cattle)	NPHP1	NP_001098802.1
Rattus norvegicus (Norway rat)	Nphp1	NP_001099976.1
Gallus gallus (chicken)	NPHP1	NP_001186358.1
Canis lupus familiaris (dog)	NPHP1	NP_001182770.1
Xenopus tropicalis (tropical clawed frog)	nphp1	XP_002940386.2
Homo sapiens (human)	NPHP1	NP_001121650.1
Mus musculus (house mouse)	Nphp1	NP_058598.1

Gene Ontology
Bibliography

Related articles in PubMed

Copy-Number Variation Contributes to the Mutational Load of Bardet-Biedl Syndrome.
Lindstrand A, Frangakis S, Carvalho CM, Richardson EB, McFadden KA, Willer JR, Pehlivan D, Liu P, Pediaditakis IL, Sabo A, Lewis RA, Banin E, Lupski JR, Davis EE, Katsanis N
American journal of human genetics99(2)318-36(2016 Aug)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Recurrent CNVs and SNVs at the NPHP1 locus contribute pathogenic alleles to Bardet-Biedl syndrome.
Lindstrand A, Davis EE, Carvalho CM, Pehlivan D, Willer JR, Tsai IC, Ramanathan S, Zuppan C, Sabo A, Muzny D, Gibbs R, Liu P, Lewis RA, Banin E, Lupski JR, Clark R, Katsanis N
American journal of human genetics94(5)745-54(2014 May)

High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
Thomas HR, Percival SM, Yoder BK, Parant JM
PloS one9(12)e114632(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nphp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nphp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02028	NM_001077170.1 Latest version!	Danio rerio nephronophthisis 1 (nphp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nphp1 ( NM_001077170.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070638.1 Danio rerio nephronophthisis 1 (nphp1), mRNA.

Price & Availability↑

CloneID	ODa02028
Clone ID Related Accession (Same CDS sequence)	NM_001077170.1
Accession Version	NM_001077170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-12
Organism	Danio rerio(zebrafish)
Product	nephrocystin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124181.1. On or before Oct 11, 2006 this sequence version replaced XM_682429.1, XM_703691.1, XM_703692.1. ##Evidence-Data-START## Transcript exon combination :: BC124181.1, BC165641.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCTCCAA AGAGAAGAGG GCCCCTTCAG ATTGTCCAAA GAGAAACGGA TGACATCAAG 
CGACAGGTGG ATGCTTTGGT GCAGCAAGCG GCTAAAGCAG ATGTGTCAGA AAACAAAGAG 
GCCTTGCAGA GGTGCAGAGA ATTACAGCGC TTAGTGGACG ACATTATGAA GAGGCTGAAG 
AAGTTATCTA AAGCAGATGA ACCAGCTCCA GTTGGAAACT ATGACCAGTG TAAGAGGGAA 
GAGGAGAAGC GGTTATTGAC TTTACATGAT CATCTACAGT CTCTAGCTCT TCAGCTGCTG 
CCTCATGATG AGACGGCTGG ACCTAGTTCT TCACATACAG AACAGGACAG CGAGGAAGAG 
GATAGTGATG AAGAAGATGA GAGCTCTGAA GAGGAAGATG CTAAAGGAGA CGTACACGAG 
GAAGATGATG ATGCTGACAG TGCTGATGAA GATACAGATA TGGCTGCTGC TGCAGAACAG 
GGATCTGCCT CCTATATCAC TCTCAGTTCG TTTACAGCGA AGGAGGAAGG GGATCTCAGC 
ATTCAGAAAG GAGAGATTCT AAAAGTTTTG TCAAAAAATA AAGATGGATG GTGGCTAGCG 
CAAAACTCGA ATGGACAGAA AGGCCTGGTG CCTAAAACTT TTCTAAAGAT GTCATCCTCT 
CTACAGGACA GTGAGAGAGA GAATGAAGAT GAAGAGGACA GCGAGGAAGA ACAGGAATCC 
AGCAAAACAT CCAATTGGAA CACAGTACGG AAGGCTGTAA CTGAGATTGA TGCTACAGAT 
GTGCTGTCGG CGATGGGTGC TATTCCTCCA GGCTTCAGAC CTTCAACATT ATCCAGACTT 
CTGGAGGAAG GGTCATCTTA CAGAGCAAGT CACTTTATTC AACCCAAGCT TAGCCAGTCT 
CAACTGTCAT TTAAAGATCT CCATATGGAT CCAGACACAG GAAAGGTTCA CGGTCGGCCA 
TCTAGAGTCA GTTTAACCCT GACCCTGTGG AGCTGTAGAA TGATCCCATC ACCCGGGGTC 
GGGTTACAGG TGCTTAGCAG ACACGTTCGG CTCTGTGCAT TTAACGGAAC TCAGGTTTTG 
AGTAACATCC ACACAGTCAG AGCCACTTAC AACTCCAAGA ACCCAAAGAC TTGGAGCTTT 
TCTCCACGGA TGACTGGGAT GCTACCCTGT CTACTGGATG GAGATTGTTT TCTGCGTTGC 
GACTCTGAAT CTTCCGATCT TGGAATTCTG TTTGAATTGG GTGTAACCTA CATAAGAAAC 
TCTACTGGGG AGAGAGGAGA CCTCAGCTGT GGCTGGACTT TCCTTAAACT GTTTGATGAA 
AGCGGAGCCC CCATTGCTCT ACGGACACAA GAGCTCACTA TTCATGGTGG CACGCCATTT 
GAGAAAGACT TAGATATGGA TACCATGTCT ACAAAAAAAG GGTATCAGAC TGGTGTGTTT 
CACCAGATGT TGCTCTCCAG GAAGATGCCA AAACTTGTTG TGAAGCTCAA ATCGCTAAAC 
TCAAGCAGCA GATCGCGTCT GAATCTGCTG CCTGACACTA TCGTAGGAAG TCTGTCCACA 
TTACATCTCT TGGCGACATA CAGGCAAATT CTCGCTGATG CTCTTTTACT TGACAGAGTG 
ACCATGTGTA ATGCAGATCT GATCTGCAGC TCAGTTTTAG CCTCCTTTCC TAATGTTCTA 
GATCAGCCAG ATCTGATGGA TGCATTTAGG AAATCCTGGA GCGAATCAGA GAGTAACCTG 
AAAAGATCTG AAAAGAGGGA TATAAACATA CTAAAGCAGA TGTTTGTGAG TGTCTACATG 
GGCAGCGTGT TTCCATTGCT GTTTTCTGCA GAAATGCCCA CTCCCTGCTG GGCAGATGAG 
GAAACGGAGT CTCAGCGCGC CCGTATCATC TACAACCCTT CCCAAAAAAC CTCAATAGAA 
ACTCTGCTGT CCTCCCAAAA CTCACATCAA GCCTTTGACA TCACACAGGT CACCTATGAC 
CTCATTAGCA GTGCCCGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070638.1
CDS	126..2129
Translation	MPPKRRGPLQIVQRETDDIKRQVDALVQQAAKADVSENKEALQRCRELQRLVDDIMKRLKKLSKADEPAPVGNYDQCKREEEKRLLTLHDHLQSLALQLLPHDETAGPSSSHTEQDSEEEDSDEEDESSEEEDAKGDVHEEDDDADSADEDTDMAAAAEQGSASYITLSSFTAKEEGDLSIQKGEILKVLSKNKDGWWLAQNSNGQKGLVPKTFLKMSSSLQDSERENEDEEDSEEEQESSKTSNWNTVRKAVTEIDATDVLSAMGAIPPGFRPSTLSRLLEEGSSYRASHFIQPKLSQSQLSFKDLHMDPDTGKVHGRPSRVSLTLTLWSCRMIPSPGVGLQVLSRHVRLCAFNGTQVLSNIHTVRATYNSKNPKTWSFSPRMTGMLPCLLDGDCFLRCDSESSDLGILFELGVTYIRNSTGERGDLSCGWTFLKLFDESGAPIALRTQELTIHGGTPFEKDLDMDTMSTKKGYQTGVFHQMLLSRKMPKLVVKLKSLNSSSRSRLNLLPDTIVGSLSTLHLLATYRQILADALLLDRVTMCNADLICSSVLASFPNVLDQPDLMDAFRKSWSESESNLKRSEKRDINILKQMFVSVYMGSVFPLLFSAEMPTPCWADEETESQRARIIYNPSQKTSIETLLSSQNSHQAFDITQVTYDLISSARQ

Target ORF information:

RefSeq Version	NM_001077170.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephronophthisis 1 (nphp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077170.1

ORF Insert Sequence:

ATGCCTCCAA AGAGAAGAGG GCCCCTTCAG ATTGTCCAAA GAGAAACGGA TGACATCAAG 
CGACAGGTGG ATGCTTTGGT GCAGCAAGCG GCTAAAGCAG ATGTGTCAGA AAACAAAGAG 
GCCTTGCAGA GGTGCAGAGA ATTACAGCGC TTAGTGGACG ACATTATGAA GAGGCTGAAG 
AAGTTATCTA AAGCAGATGA ACCAGCTCCA GTTGGAAACT ATGACCAGTG TAAGAGGGAA 
GAGGAGAAGC GGTTATTGAC TTTACATGAT CATCTACAGT CTCTAGCTCT TCAGCTGCTG 
CCTCATGATG AGACGGCTGG ACCTAGTTCT TCACATACAG AACAGGACAG CGAGGAAGAG 
GATAGTGATG AAGAAGATGA GAGCTCTGAA GAGGAAGATG CTAAAGGAGA CGTACACGAG 
GAAGATGATG ATGCTGACAG TGCTGATGAA GATACAGATA TGGCTGCTGC TGCAGAACAG 
GGATCTGCCT CCTATATCAC TCTCAGTTCG TTTACAGCGA AGGAGGAAGG GGATCTCAGC 
ATTCAGAAAG GAGAGATTCT AAAAGTTTTG TCAAAAAATA AAGATGGATG GTGGCTAGCG 
CAAAACTCGA ATGGACAGAA AGGCCTGGTG CCTAAAACTT TTCTAAAGAT GTCATCCTCT 
CTACAGGACA GTGAGAGAGA GAATGAAGAT GAAGAGGACA GCGAGGAAGA ACAGGAATCC 
AGCAAAACAT CCAATTGGAA CACAGTACGG AAGGCTGTAA CTGAGATTGA TGCTACAGAT 
GTGCTGTCGG CGATGGGTGC TATTCCTCCA GGCTTCAGAC CTTCAACATT ATCCAGACTT 
CTGGAGGAAG GGTCATCTTA CAGAGCAAGT CACTTTATTC AACCCAAGCT TAGCCAGTCT 
CAACTGTCAT TTAAAGATCT CCATATGGAT CCAGACACAG GAAAGGTTCA CGGTCGGCCA 
TCTAGAGTCA GTTTAACCCT GACCCTGTGG AGCTGTAGAA TGATCCCATC ACCCGGGGTC 
GGGTTACAGG TGCTTAGCAG ACACGTTCGG CTCTGTGCAT TTAACGGAAC TCAGGTTTTG 
AGTAACATCC ACACAGTCAG AGCCACTTAC AACTCCAAGA ACCCAAAGAC TTGGAGCTTT 
TCTCCACGGA TGACTGGGAT GCTACCCTGT CTACTGGATG GAGATTGTTT TCTGCGTTGC 
GACTCTGAAT CTTCCGATCT TGGAATTCTG TTTGAATTGG GTGTAACCTA CATAAGAAAC 
TCTACTGGGG AGAGAGGAGA CCTCAGCTGT GGCTGGACTT TCCTTAAACT GTTTGATGAA 
AGCGGAGCCC CCATTGCTCT ACGGACACAA GAGCTCACTA TTCATGGTGG CACGCCATTT 
GAGAAAGACT TAGATATGGA TACCATGTCT ACAAAAAAAG GGTATCAGAC TGGTGTGTTT 
CACCAGATGT TGCTCTCCAG GAAGATGCCA AAACTTGTTG TGAAGCTCAA ATCGCTAAAC 
TCAAGCAGCA GATCGCGTCT GAATCTGCTG CCTGACACTA TCGTAGGAAG TCTGTCCACA 
TTACATCTCT TGGCGACATA CAGGCAAATT CTCGCTGATG CTCTTTTACT TGACAGAGTG 
ACCATGTGTA ATGCAGATCT GATCTGCAGC TCAGTTTTAG CCTCCTTTCC TAATGTTCTA 
GATCAGCCAG ATCTGATGGA TGCATTTAGG AAATCCTGGA GCGAATCAGA GAGTAACCTG 
AAAAGATCTG AAAAGAGGGA TATAAACATA CTAAAGCAGA TGTTTGTGAG TGTCTACATG 
GGCAGCGTGT TTCCATTGCT GTTTTCTGCA GAAATGCCCA CTCCCTGCTG GGCAGATGAG 
GAAACGGAGT CTCAGCGCGC CCGTATCATC TACAACCCTT CCCAAAAAAC CTCAATAGAA 
ACTCTGCTGT CCTCCCAAAA CTCACATCAA GCCTTTGACA TCACACAGGT CACCTATGAC 
CTCATTAGCA GTGCCCGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Copy-Number Variation Contributes to the Mutational Load of Bardet-Biedl Syndrome.
American journal of human genetics99(2)318-36(2016 Aug)
Lindstrand A,Frangakis S,Carvalho CM,Richardson EB,McFadden KA,Willer JR,Pehlivan D,Liu P,Pediaditakis IL,Sabo A,Lewis RA,Banin E,Lupski JR,Davis EE,Katsanis N

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Recurrent CNVs and SNVs at the NPHP1 locus contribute pathogenic alleles to Bardet-Biedl syndrome.
American journal of human genetics94(5)745-54(2014 May)
Lindstrand A,Davis EE,Carvalho CM,Pehlivan D,Willer JR,Tsai IC,Ramanathan S,Zuppan C,Sabo A,Muzny D,Gibbs R,Liu P,Lewis RA,Banin E,Lupski JR,Clark R,Katsanis N

High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
PloS one9(12)e114632(2014)
Thomas HR,Percival SM,Yoder BK,Parant JM