gaa cDNA ORF clone, Danio rerio(zebrafish)

The following gaa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gaa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa08170 XM_001921922.5
Latest version!
Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa47673 XM_009299343.2
Latest version!
Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa141736 XM_021473369.1
Latest version!
Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
ODa58211 XM_017354744.1
Latest version!
Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
ODa08170 XM_001921922.4 Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa08170
    Clone ID Related Accession (Same CDS sequence) XM_001921922.5 , XM_001921922.4
    Accession Version XM_001921922.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lysosomal alpha-glucosidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001921922.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
    GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT
    TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT
    CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT
    TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA
    TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC
    ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG
    CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT
    CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC
    AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT
    GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT
    CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG
    GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA
    TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC
    CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG
    GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC
    TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA
    GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT
    ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG
    GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG
    CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT
    GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT
    GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG
    AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG
    TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC
    ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG
    GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT
    GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC
    GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA
    TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA
    AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC
    ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT
    GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT
    AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG
    ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC
    TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG
    AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG
    GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC
    CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC
    GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA
    TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA
    GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT
    CTTCTCTTCT TTGCTGAAGA GTCATATGTG GTCAGTAAAC CGATCAAATT AAACGGATCT
    CTTGATGGTT TGGTCCTGGG GGAAGTGCGT GTTTTCGGGG TCCAGACTCC ACCTACTGCC
    GTATGGGCCA ACGGACAGAA AGTTCCTGAC TTCTCATACA GATTAGATAC CAAGGTTCTG
    ACAGTAGCTG GTCTGGAACT ACCAATGACT GCCAAGATCA CAATACAGTG GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001921957.2
    CDS259..3015
    Translation

    Target ORF information:

    RefSeq Version XM_001921922.5
    Organism Danio rerio(zebrafish)
    Definition Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001921922.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
    GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT
    TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT
    CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT
    TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA
    TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC
    ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG
    CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT
    CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC
    AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT
    GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT
    CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG
    GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA
    TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC
    CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG
    GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC
    TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA
    GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT
    ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG
    GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG
    CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT
    GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT
    GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG
    AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG
    TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC
    ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG
    GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT
    GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC
    GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA
    TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA
    AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC
    ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT
    GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT
    AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG
    ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC
    TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG
    AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG
    GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC
    CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC
    GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA
    TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA
    GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT
    CTTCTCTTCT TTGCTGAAGA GTCATATGTG GTCAGTAAAC CGATCAAATT AAACGGATCT
    CTTGATGGTT TGGTCCTGGG GGAAGTGCGT GTTTTCGGGG TCCAGACTCC ACCTACTGCC
    GTATGGGCCA ACGGACAGAA AGTTCCTGAC TTCTCATACA GATTAGATAC CAAGGTTCTG
    ACAGTAGCTG GTCTGGAACT ACCAATGACT GCCAAGATCA CAATACAGTG GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa08170
    Clone ID Related Accession (Same CDS sequence) XM_001921922.5 , XM_001921922.4
    Accession Version XM_001921922.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lysosomal alpha-glucosidase isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001921922.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
    GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT
    TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT
    CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT
    TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA
    TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC
    ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG
    CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT
    CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC
    AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT
    GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT
    CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG
    GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA
    TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC
    CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG
    GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC
    TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA
    GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT
    ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG
    GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG
    CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT
    GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT
    GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG
    AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG
    TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC
    ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG
    GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT
    GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC
    GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA
    TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA
    AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC
    ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT
    GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT
    AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG
    ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC
    TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG
    AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG
    GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC
    CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC
    GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA
    TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA
    GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT
    CTTCTCTTCT TTGCTGAAGA GTCATATGTG GTCAGTAAAC CGATCAAATT AAACGGATCT
    CTTGATGGTT TGGTCCTGGG GGAAGTGCGT GTTTTCGGGG TCCAGACTCC ACCTACTGCC
    GTATGGGCCA ACGGACAGAA AGTTCCTGAC TTCTCATACA GATTAGATAC CAAGGTTCTG
    ACAGTAGCTG GTCTGGAACT ACCAATGACT GCCAAGATCA CAATACAGTG GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001921957.2
    CDS467..3223
    Translation

    Target ORF information:

    RefSeq Version XM_001921922.4
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001921922.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
    GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT
    TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT
    CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT
    TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA
    TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC
    ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG
    CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT
    CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC
    AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT
    GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT
    CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG
    GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA
    TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC
    CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG
    GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC
    TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA
    GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT
    ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG
    GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG
    CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT
    GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT
    GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG
    AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG
    TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC
    ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG
    GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT
    GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC
    GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA
    TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA
    AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC
    ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT
    GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT
    AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG
    ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC
    TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG
    AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG
    GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC
    CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC
    GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA
    TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA
    GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT
    CTTCTCTTCT TTGCTGAAGA GTCATATGTG GTCAGTAAAC CGATCAAATT AAACGGATCT
    CTTGATGGTT TGGTCCTGGG GGAAGTGCGT GTTTTCGGGG TCCAGACTCC ACCTACTGCC
    GTATGGGCCA ACGGACAGAA AGTTCCTGAC TTCTCATACA GATTAGATAC CAAGGTTCTG
    ACAGTAGCTG GTCTGGAACT ACCAATGACT GCCAAGATCA CAATACAGTG GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47673
    Clone ID Related Accession (Same CDS sequence) XM_009299343.2
    Accession Version XM_009299343.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lysosomal alpha-glucosidase isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
    GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT
    TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT
    CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT
    TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA
    TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC
    ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG
    CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT
    CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC
    AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT
    GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT
    CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG
    GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA
    TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC
    CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG
    GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC
    TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA
    GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT
    ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG
    GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG
    CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT
    GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT
    GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG
    AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG
    TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGACATGA ATGAGCCTTC AAATTTTGTC
    CAGGGCTCTG TGGATGGATG TCCTGACTCT GAATTGGAGA AACCACCATA TACACCAGGT
    GTGATTGGAG GCCAGTTAAA CTCAGGAACT CTGTGTGTGT CCGCACAGCA GTATCTGTCC
    AATCATTACA ATCTACACAA CCTCTATGGG CTGACCGAAG CCATCGCCAC TCACAGGGCA
    CTGCTAAAAG TGAAGAAAAC TCGGCCGTTT GTTTTATCTC GATCATCTTT TCCGGGACTG
    GGTCGTTTCT CTGCACACTG GACAGGAGAT GTGCGAAGTG ACTGGGAACA ACTGCGCTTT
    TCAATACCCG CTGTGCTCCT GTTTGGACTG TATGGCATTC CATTAGTGGG AGCTGATGTT
    TGTGGATTTG GTGGAGACAC TAACGAAGAG CTGTGTGTGC GCTGGACACA ACTTGGAGCC
    TTTTACCCCT TCATGAGAAA CCACAATGAC AGACCTAATG CTCCTCAGGA GCCATATGTA
    TTTAGTCAGC GAGCCCAGGA TGCCATGAGG ATGGTGATTA ATCTTCGATA TTCTCTCCTG
    CCCTTCCTCT ACACACTCTT CCATCACGCG CACACCTCCG CCAGCACCGT GGCCCGCCCT
    CTCTTCCTTC AGTTCCCCAC TGATCCAGAT TGCAGGAGCA TTGACAGACA GTTCCTGTGG
    GGGAGTTCAC TGCTCATCAG TCCAGTTCTG GAGCAGGGAG CAGTGGAGGT GATGGCGTAT
    CTCCCCCCGG GGACTTGGTA CAGTCTGCAC AATGGCCAAA CATATTACAG TAAAGGACAA
    TACATTGTGT TTCCTGCTTC GTTGGACACC ATCATCGTTC ACGTGAGAGA GAGGAGCATC
    ATCCCACAGC AGGCTCCTGC TCTGACCACT GCAATATCTC GCAAAAACCC TTTCACCCTA
    ACGGTGGGAC TGTCAGTGGG AAATTTGGCC AAAGGAGAAC TTTTTTGGGA TGATGGAGAA
    AGTCTGGATA CTTTTGAGCG GGGAGATTAT TCATATCTTC TCTTCTTTGC TGAAGAGTCA
    TATGTGGTCA GTAAACCGAT CAAATTAAAC GGATCTCTTG ATGGTTTGGT CCTGGGGGAA
    GTGCGTGTTT TCGGGGTCCA GACTCCACCT ACTGCCGTAT GGGCCAACGG ACAGAAAGTT
    CCTGACTTCT CATACAGATT AGATACCAAG GTTCTGACAG TAGCTGGTCT GGAACTACCA
    ATGACTGCCA AGATCACAAT ACAGTGGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297618.1
    CDS467..3199
    Translation

    Target ORF information:

    RefSeq Version XM_009299343.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299343.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
    GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT
    TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT
    CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT
    TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA
    TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC
    ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG
    CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT
    CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC
    AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT
    GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT
    CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG
    GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA
    TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC
    CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG
    GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC
    TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA
    GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT
    ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG
    GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG
    CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT
    GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT
    GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG
    AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG
    TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGACATGA ATGAGCCTTC AAATTTTGTC
    CAGGGCTCTG TGGATGGATG TCCTGACTCT GAATTGGAGA AACCACCATA TACACCAGGT
    GTGATTGGAG GCCAGTTAAA CTCAGGAACT CTGTGTGTGT CCGCACAGCA GTATCTGTCC
    AATCATTACA ATCTACACAA CCTCTATGGG CTGACCGAAG CCATCGCCAC TCACAGGGCA
    CTGCTAAAAG TGAAGAAAAC TCGGCCGTTT GTTTTATCTC GATCATCTTT TCCGGGACTG
    GGTCGTTTCT CTGCACACTG GACAGGAGAT GTGCGAAGTG ACTGGGAACA ACTGCGCTTT
    TCAATACCCG CTGTGCTCCT GTTTGGACTG TATGGCATTC CATTAGTGGG AGCTGATGTT
    TGTGGATTTG GTGGAGACAC TAACGAAGAG CTGTGTGTGC GCTGGACACA ACTTGGAGCC
    TTTTACCCCT TCATGAGAAA CCACAATGAC AGACCTAATG CTCCTCAGGA GCCATATGTA
    TTTAGTCAGC GAGCCCAGGA TGCCATGAGG ATGGTGATTA ATCTTCGATA TTCTCTCCTG
    CCCTTCCTCT ACACACTCTT CCATCACGCG CACACCTCCG CCAGCACCGT GGCCCGCCCT
    CTCTTCCTTC AGTTCCCCAC TGATCCAGAT TGCAGGAGCA TTGACAGACA GTTCCTGTGG
    GGGAGTTCAC TGCTCATCAG TCCAGTTCTG GAGCAGGGAG CAGTGGAGGT GATGGCGTAT
    CTCCCCCCGG GGACTTGGTA CAGTCTGCAC AATGGCCAAA CATATTACAG TAAAGGACAA
    TACATTGTGT TTCCTGCTTC GTTGGACACC ATCATCGTTC ACGTGAGAGA GAGGAGCATC
    ATCCCACAGC AGGCTCCTGC TCTGACCACT GCAATATCTC GCAAAAACCC TTTCACCCTA
    ACGGTGGGAC TGTCAGTGGG AAATTTGGCC AAAGGAGAAC TTTTTTGGGA TGATGGAGAA
    AGTCTGGATA CTTTTGAGCG GGGAGATTAT TCATATCTTC TCTTCTTTGC TGAAGAGTCA
    TATGTGGTCA GTAAACCGAT CAAATTAAAC GGATCTCTTG ATGGTTTGGT CCTGGGGGAA
    GTGCGTGTTT TCGGGGTCCA GACTCCACCT ACTGCCGTAT GGGCCAACGG ACAGAAAGTT
    CCTGACTTCT CATACAGATT AGATACCAAG GTTCTGACAG TAGCTGGTCT GGAACTACCA
    ATGACTGCCA AGATCACAAT ACAGTGGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141736
    Clone ID Related Accession (Same CDS sequence) XM_021473369.1
    Accession Version XM_021473369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lysosomal alpha-glucosidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCACCAC ATAGAAGTGC CAACCTGTAT GGCTCCCACC CTTTCTTCCT GGTCCAAGAA 
    GGTGATGGAC AGGCACATGG AGTCTTCCTT CTCAACAGCA ATGCAATGGA AGTGTTTATG
    CAGCCTGCTC CAGCATTAAC ATGGGTGACC ATTGGAGGAA TTCTGGACTT CTTCATCTTC
    TTGGGCCCGA GTCCTCAGAG TGTGATTCAA CAGTATCAGG AGGTTATAGG ATATCCTATG
    ATGCCTCCCT ACTGGTCACT GGGTTTTCAT CTGTGTCGTT GGGGTTATAC CTCCACCAAC
    ATCACTAGGA CAGTAGTTCA GCTCATGCGG CAGGCCAAGA TACCTTTGGA TGTACAGTGG
    AATGACCTTG ATTATGCAGA CCAAAGAAGA GTCTTTACTT TCGACCCGCA GCGGTTTGGA
    GATTTGCCTC AAATGGTGGA AGATTTCCAT CAGCTGGGGA TGAAATATGT GCTCATTCTT
    GATCCAGGCA TCAGCAGCGC GAGTCCTCCT GGTTCATACA AACCGTTTGA CGATGGACTG
    AAGAAAGGAG TTTTCATCAA CAACTCCACT GGACAGATCC TTATTGGGAA GGTATGGCCG
    GGTCCGACTG CATTCCCAGA TTTCACCAAT CCTACAACAC AGGACTGGTG GATGGACTGG
    ATCAAAGACT TTTACAACAA GGTACCTGTG GATGGACTTT GGATAGACAT GAATGAGCCT
    TCAAATTTTG TCCAGGGCTC TGTGGATGGA TGTCCTGACT CTGAATTGGA GAAACCACCA
    TATACACCAG GTGTGATTGG AGGCCAGTTA AACTCAGGAA CTCTGTGTGT GTCCGCACAG
    CAGTATCTGT CCAATCATTA CAATCTACAC AACCTCTATG GGCTGACCGA AGCCATCGCC
    ACTCACAGGG CACTGCTAAA AGTGAAGAAA ACTCGGCCGT TTGTTTTATC TCGATCATCT
    TTTCCGGGAC TGGGTCGTTT CTCTGCACAC TGGACAGGAG ATGTGCGAAG TGACTGGGAA
    CAACTGCGCT TTTCAATACC CGCTGTGCTC CTGTTTGGAC TGTATGGCAT TCCATTAGTG
    GGAGCTGATG TTTGTGGATT TGGTGGAGAC ACTAACGAAG AGCTGTGTGT GCGCTGGACA
    CAACTTGGAG CCTTTTACCC CTTCATGAGA AACCACAATG ACAGACCTAA TGCTCCTCAG
    GAGCCATATG TATTTAGTCA GCGAGCCCAG GATGCCATGA GGATGGTGAT TAATCTTCGA
    TATTCTCTCC TGCCCTTCCT CTACACACTC TTCCATCACG CGCACACCTC CGCCAGCACC
    GTGGCCCGCC CTCTCTTCCT TCAGTTCCCC ACTGATCCAG ATTGCAGGAG CATTGACAGA
    CAGTTCCTGT GGGGGAGTTC ACTGCTCATC AGTCCAGTTC TGGAGCAGGG AGCAGTGGAG
    GTGATGGCGT ATCTCCCCCC GGGGACTTGG TACAGTCTGC ACAATGGCCA AACATATTAC
    AGTAAAGGAC AATACATTGT GTTTCCTGCT TCGTTGGACA CCATCATCGT TCACGTGAGA
    GAGAGGAGCA TCATCCCACA GCAGGCTCCT GCTCTGACCA CTGCAATATC TCGCAAAAAC
    CCTTTCACCC TAACGGTGGG ACTGTCAGTG GGAAATTTGG CCAAAGGAGA ACTTTTTTGG
    GATGATGGAG AAAGTCTGGA TACTTTTGAG CGGGGAGATT ATTCATATCT TCTCTTCTTT
    GCTGAAGAGT CATATGTGGT CAGTAAACCG ATCAAATTAA ACGGATCTCT TGATGGTTTG
    GTCCTGGGGG AAGTGCGTGT TTTCGGGGTC CAGACTCCAC CTACTGCCGT ATGGGCCAAC
    GGACAGAAAG TTCCTGACTT CTCATACAGA TTAGATACCA AGGTTCTGAC AGTAGCTGGT
    CTGGAACTAC CAATGACTGC CAAGATCACA ATACAGTGGA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329044.1
    CDS219..2183
    Translation

    Target ORF information:

    RefSeq Version XM_021473369.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCACCAC ATAGAAGTGC CAACCTGTAT GGCTCCCACC CTTTCTTCCT GGTCCAAGAA 
    GGTGATGGAC AGGCACATGG AGTCTTCCTT CTCAACAGCA ATGCAATGGA AGTGTTTATG
    CAGCCTGCTC CAGCATTAAC ATGGGTGACC ATTGGAGGAA TTCTGGACTT CTTCATCTTC
    TTGGGCCCGA GTCCTCAGAG TGTGATTCAA CAGTATCAGG AGGTTATAGG ATATCCTATG
    ATGCCTCCCT ACTGGTCACT GGGTTTTCAT CTGTGTCGTT GGGGTTATAC CTCCACCAAC
    ATCACTAGGA CAGTAGTTCA GCTCATGCGG CAGGCCAAGA TACCTTTGGA TGTACAGTGG
    AATGACCTTG ATTATGCAGA CCAAAGAAGA GTCTTTACTT TCGACCCGCA GCGGTTTGGA
    GATTTGCCTC AAATGGTGGA AGATTTCCAT CAGCTGGGGA TGAAATATGT GCTCATTCTT
    GATCCAGGCA TCAGCAGCGC GAGTCCTCCT GGTTCATACA AACCGTTTGA CGATGGACTG
    AAGAAAGGAG TTTTCATCAA CAACTCCACT GGACAGATCC TTATTGGGAA GGTATGGCCG
    GGTCCGACTG CATTCCCAGA TTTCACCAAT CCTACAACAC AGGACTGGTG GATGGACTGG
    ATCAAAGACT TTTACAACAA GGTACCTGTG GATGGACTTT GGATAGACAT GAATGAGCCT
    TCAAATTTTG TCCAGGGCTC TGTGGATGGA TGTCCTGACT CTGAATTGGA GAAACCACCA
    TATACACCAG GTGTGATTGG AGGCCAGTTA AACTCAGGAA CTCTGTGTGT GTCCGCACAG
    CAGTATCTGT CCAATCATTA CAATCTACAC AACCTCTATG GGCTGACCGA AGCCATCGCC
    ACTCACAGGG CACTGCTAAA AGTGAAGAAA ACTCGGCCGT TTGTTTTATC TCGATCATCT
    TTTCCGGGAC TGGGTCGTTT CTCTGCACAC TGGACAGGAG ATGTGCGAAG TGACTGGGAA
    CAACTGCGCT TTTCAATACC CGCTGTGCTC CTGTTTGGAC TGTATGGCAT TCCATTAGTG
    GGAGCTGATG TTTGTGGATT TGGTGGAGAC ACTAACGAAG AGCTGTGTGT GCGCTGGACA
    CAACTTGGAG CCTTTTACCC CTTCATGAGA AACCACAATG ACAGACCTAA TGCTCCTCAG
    GAGCCATATG TATTTAGTCA GCGAGCCCAG GATGCCATGA GGATGGTGAT TAATCTTCGA
    TATTCTCTCC TGCCCTTCCT CTACACACTC TTCCATCACG CGCACACCTC CGCCAGCACC
    GTGGCCCGCC CTCTCTTCCT TCAGTTCCCC ACTGATCCAG ATTGCAGGAG CATTGACAGA
    CAGTTCCTGT GGGGGAGTTC ACTGCTCATC AGTCCAGTTC TGGAGCAGGG AGCAGTGGAG
    GTGATGGCGT ATCTCCCCCC GGGGACTTGG TACAGTCTGC ACAATGGCCA AACATATTAC
    AGTAAAGGAC AATACATTGT GTTTCCTGCT TCGTTGGACA CCATCATCGT TCACGTGAGA
    GAGAGGAGCA TCATCCCACA GCAGGCTCCT GCTCTGACCA CTGCAATATC TCGCAAAAAC
    CCTTTCACCC TAACGGTGGG ACTGTCAGTG GGAAATTTGG CCAAAGGAGA ACTTTTTTGG
    GATGATGGAG AAAGTCTGGA TACTTTTGAG CGGGGAGATT ATTCATATCT TCTCTTCTTT
    GCTGAAGAGT CATATGTGGT CAGTAAACCG ATCAAATTAA ACGGATCTCT TGATGGTTTG
    GTCCTGGGGG AAGTGCGTGT TTTCGGGGTC CAGACTCCAC CTACTGCCGT ATGGGCCAAC
    GGACAGAAAG TTCCTGACTT CTCATACAGA TTAGATACCA AGGTTCTGAC AGTAGCTGGT
    CTGGAACTAC CAATGACTGC CAAGATCACA ATACAGTGGA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58211
    Clone ID Related Accession (Same CDS sequence) XM_017354744.1
    Accession Version XM_017354744.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lysosomal alpha-glucosidase isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
    GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT
    TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT
    CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT
    TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA
    TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC
    ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG
    CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT
    CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC
    AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT
    GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT
    CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG
    GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA
    TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC
    CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG
    GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC
    TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA
    GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT
    ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG
    GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG
    CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT
    GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT
    GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG
    AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG
    TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC
    ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG
    GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT
    GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC
    GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA
    TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA
    AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC
    ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT
    GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT
    AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG
    ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC
    TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG
    AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG
    GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC
    CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC
    GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA
    TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA
    GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT
    CTTCTCTTCT TTGCTGAAGA GGTTCTGACA GTAGCTGGTC TGGAACTACC AATGACTGCC
    AAGATCACAA TACAGTGGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210233.1
    CDS467..3070
    Translation

    Target ORF information:

    RefSeq Version XM_017354744.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354744.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
    GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT
    TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT
    CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT
    TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA
    TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC
    ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG
    CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT
    CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC
    AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT
    GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT
    CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG
    GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA
    TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC
    CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG
    GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC
    TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA
    GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT
    ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG
    GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG
    CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT
    GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT
    GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG
    AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG
    TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC
    ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG
    GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT
    GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC
    GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA
    TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA
    AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC
    ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT
    GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT
    AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG
    ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC
    TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG
    AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG
    GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC
    CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC
    GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA
    TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA
    GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT
    CTTCTCTTCT TTGCTGAAGA GGTTCTGACA GTAGCTGGTC TGGAACTACC AATGACTGCC
    AAGATCACAA TACAGTGGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.