med16 cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

Gene
Clones
gRNAs

Summary

Gene Symbol	med16
Entrez Gene ID	107100500
Full Name	mediator complex subunit 16
Gene Type	protein-coding
Organism	Cyprinodon variegatus(sheepshead minnow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015399004.1	XP_015254490.1	mediator of RNA polymerase II transcription subunit 16 isoform X2
XM_015399003.1	XP_015254489.1	mediator of RNA polymerase II transcription subunit 16 isoform X1

Pathways from BioSystems
Gene Ontology

The following med16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCy27061	XM_015399003.1 Latest version!	Cyprinodon variegatus mediator complex subunit 16 (med16), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCy27062	XM_015399004.1 Latest version!	Cyprinodon variegatus mediator complex subunit 16 (med16), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

med16 ( XM_015399003.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015254489.1 Cyprinodon variegatus mediator complex subunit 16 (med16), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCy27061
Clone ID Related Accession (Same CDS sequence)	XM_015399003.1
Accession Version	XM_015399003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	mediator of RNA polymerase II transcription subunit 16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015151433.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG CTTATGTGTG TGAGTGGGAG AAACGTCCAA AAAGCACCCA CTGCCCATCC 
ATCCCTCTGG TCTGCTCCTG GTCCTGCAGG AACCTGATAG CCTTCACCAC AGACCTGAAG 
AATGACGACG ATGACAAAGA TGTCAGTCAC ATGATCCACA TCATCGACAC CGAGCACCCC 
TGGGACATGT TCTCCATCAA CTCTGGCCAC AGTGAGGTCA TTTCCTGTCT GGAGTGGGAC 
CAGTCAGGTT CTCGCCTGCT GTCCGCTGAT GGTGACGGTC AGATTAAGTG CTGGTCCATG 
TCGGACCACC TGGTCAACAG CTGGGAGTGC GCCTTCTCCA GCTCTGTGGA TGGAGATCCC 
ATCGTGGCTC TGAGCTGGCT GCACAACGGT GTCAAACTGG CCCTGCATGT CGAGATGTCC 
GGCTCCACAA ACTTCGGGGA GAAGTTTTCC AGGGTAAAGT TCTCTCCGTC TATGACTCTT 
TTCGGCGGGA AGCCGATGGA GGGCTGGTTG GCGGTGACGG TGAGCGGTCT GGTCAGCGTG 
TCGCTGCTGA AGCCGGGCGG CGCTCTGCTG ACGGCCAGCG AGAGCCTGTG CCGGCTGAGA 
GGCCGCGTGG CCTCGGCCGA CATCGCCTTC ACCGGCGGCG GGAACATCGT GGTGGCGGCC 
ACCGATGGCA GCAGCTCCTC CCCGGTCCAG TTCTACAAGG TGCAGGTGAG CGTTGTGAGC 
GAGAAATGTC GCATCGACAC CGAGCTGCTG CCATCGCTGT TCCTGCGCTG CACCACCGAC 
CCCATGAGGA GGGACAAGTA CCCAGCCGTC ACCCACCTCA AGTTCCTGAC CCGGGAGAAC 
TCGGAGCAGG TGCTGCTGTG TGCGTCCAAT CAGAGCGGCA GCATCGTGGA GTGCTGGTCG 
CTGAGGAAGG AGGGACTTCC TGTTAATAAC ATCTTCCAGC ACCGCTCTCC AGTTGTGGGC 
GAGAAGCAGC CGACCATCCT GAAGTGGCGC ATCCTGACGA CCACCAGCGA CCTGGAGCGC 
GTTTCCACCA TGGCTCTACC CAAACTGCCC ATCTCCACCT CCAACACCGA CCTGAAGGTG 
GCCTCAGACA CCAAGTTCTT TCCTGGGCTC GGTCTGGCTC TGGCCTTCCA CGACGGCAGC 
ATCCAGATCC TCCATCGCCT CTCCCTCCAC ACCATGGCAG TCTTCTATGG CTCCTCCTCC 
GCCCAGCGGC CTGGAGACGA GGCAGCTATC AAACGGCAGA GGACCGCAGG TCCCGCCCTC 
CATTTCAAGG CCCTGCAGTT ATCCTGGACC TCATTGGCCC TAGCTGGAGT TGACAGCCAC 
GGAAAGCTCC ACATGCTGCG AGTGTCGCCC TCTATGGGCC AAGTGTTGGA CATGAACACC 
ACACTGCGCC ACCTGCTGTT TCTGCTAGAG TACTGCATGG TGACAGGCTA TGACTGGTGG 
GACGTTCTGC TGCACGTTCA GCCCACTATG GTCCACAACC TGGTGGAGAA GCTGCACGAG 
GAGTACATGA GGCAGAACCA GGCGCTACAG CAGGTTCTGG CGACACGCAT CGTAGCCGTC 
AAGGCCTCCC TCTGTAAACT CTCCACGGCA ACGGCTGCGC GAGCGTGTGA CTTTCACGCC 
AAACTGCTGC TGATCGCCAT CAGCTCCACC TTAAAGTCTC TGCTCAGACC TCACGTCCTC 
AACACGCCCG ACAAGAGTCC AGGTGACCGA CTGACGGAGA TCTGCGCCAA GAACACAGAC 
ACAGACATTG ATAAGGTGAT GATCAATCTG AAGACGGAGG AGTTTGTTCT GGATGGACCT 
CCTCTGCAGT CGTTGCAGCA GCTCATCCAG TGGGTGGGAG ACTTTGTCCT GTACCTGCTG 
GCAAACCTGC CCAACCAGGT GAGCCAGGGC TCCATTGTGC GGCCCGGTTT TGGCTTCATG 
AGAGACGGGG CATCTTTGGG GATGCTGAGG GAGATGCTGG TGATGATCCG GATCTGGGGG 
CTGCTGAAGC CCGGCTGCCT CCCCACTTTC ACGGCCATGT CAGACAACCA GGACAGCATG 
CAGCTGCTGT TCAGACTGCT CACCAAGCTG TGGCTGTGCT CTCGGGAGGA TGGCACCTCC 
CAGGAACCTG ACGAGAGCCT AATAGACGAA TGTTGCCTTC TGCCCAGCCA GCTGCTGGTT 
CCCAGTATGG ACTGGCTCCC AGTGAACGAT GGGATCATTG TGAAGCTGCA GGGTAAGCTC 
CCCCTCCGGC TGCAGTTTGG AAGAGCGTCA TCGCTGCAGG TAGTGGGAGG CACCGCCCCC 
CTGGACGTCT TCACCAGGAG TCCCGGAGCT CAGAAGATGG ACAATCTGCG CTGCATCCAC 
ATGGGCGTCA GTCCCACCGA GGAGAGTAAA GTGTGCACCA GGTGCGGCTG TGTGACGATG 
CTGCGATCTC CCAACAAGAC GAACGCCATG AAGCAGTGGG AGCAGCGCTG GATCAAGAAC 
TGCCTCTGTG GGGGTCTGTG GAGGAGGATC CCGCCAACGT TCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015254489.1
CDS	395..2902
Translation	MELAYVCEWEKRPKSTHCPSIPLVCSWSCRNLIAFTTDLKNDDDDKDVSHMIHIIDTEHPWDMFSINSGHSEVISCLEWDQSGSRLLSADGDGQIKCWSMSDHLVNSWECAFSSSVDGDPIVALSWLHNGVKLALHVEMSGSTNFGEKFSRVKFSPSMTLFGGKPMEGWLAVTVSGLVSVSLLKPGGALLTASESLCRLRGRVASADIAFTGGGNIVVAATDGSSSSPVQFYKVQVSVVSEKCRIDTELLPSLFLRCTTDPMRRDKYPAVTHLKFLTRENSEQVLLCASNQSGSIVECWSLRKEGLPVNNIFQHRSPVVGEKQPTILKWRILTTTSDLERVSTMALPKLPISTSNTDLKVASDTKFFPGLGLALAFHDGSIQILHRLSLHTMAVFYGSSSAQRPGDEAAIKRQRTAGPALHFKALQLSWTSLALAGVDSHGKLHMLRVSPSMGQVLDMNTTLRHLLFLLEYCMVTGYDWWDVLLHVQPTMVHNLVEKLHEEYMRQNQALQQVLATRIVAVKASLCKLSTATAARACDFHAKLLLIAISSTLKSLLRPHVLNTPDKSPGDRLTEICAKNTDTDIDKVMINLKTEEFVLDGPPLQSLQQLIQWVGDFVLYLLANLPNQVSQGSIVRPGFGFMRDGASLGMLREMLVMIRIWGLLKPGCLPTFTAMSDNQDSMQLLFRLLTKLWLCSREDGTSQEPDESLIDECCLLPSQLLVPSMDWLPVNDGIIVKLQGKLPLRLQFGRASSLQVVGGTAPLDVFTRSPGAQKMDNLRCIHMGVSPTEESKVCTRCGCVTMLRSPNKTNAMKQWEQRWIKNCLCGGLWRRIPPTFS

Target ORF information:

RefSeq Version	XM_015399003.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus mediator complex subunit 16 (med16), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015399003.1

ORF Insert Sequence:

ATGGAACTGG CTTATGTGTG TGAGTGGGAG AAACGTCCAA AAAGCACCCA CTGCCCATCC 
ATCCCTCTGG TCTGCTCCTG GTCCTGCAGG AACCTGATAG CCTTCACCAC AGACCTGAAG 
AATGACGACG ATGACAAAGA TGTCAGTCAC ATGATCCACA TCATCGACAC CGAGCACCCC 
TGGGACATGT TCTCCATCAA CTCTGGCCAC AGTGAGGTCA TTTCCTGTCT GGAGTGGGAC 
CAGTCAGGTT CTCGCCTGCT GTCCGCTGAT GGTGACGGTC AGATTAAGTG CTGGTCCATG 
TCGGACCACC TGGTCAACAG CTGGGAGTGC GCCTTCTCCA GCTCTGTGGA TGGAGATCCC 
ATCGTGGCTC TGAGCTGGCT GCACAACGGT GTCAAACTGG CCCTGCATGT CGAGATGTCC 
GGCTCCACAA ACTTCGGGGA GAAGTTTTCC AGGGTAAAGT TCTCTCCGTC TATGACTCTT 
TTCGGCGGGA AGCCGATGGA GGGCTGGTTG GCGGTGACGG TGAGCGGTCT GGTCAGCGTG 
TCGCTGCTGA AGCCGGGCGG CGCTCTGCTG ACGGCCAGCG AGAGCCTGTG CCGGCTGAGA 
GGCCGCGTGG CCTCGGCCGA CATCGCCTTC ACCGGCGGCG GGAACATCGT GGTGGCGGCC 
ACCGATGGCA GCAGCTCCTC CCCGGTCCAG TTCTACAAGG TGCAGGTGAG CGTTGTGAGC 
GAGAAATGTC GCATCGACAC CGAGCTGCTG CCATCGCTGT TCCTGCGCTG CACCACCGAC 
CCCATGAGGA GGGACAAGTA CCCAGCCGTC ACCCACCTCA AGTTCCTGAC CCGGGAGAAC 
TCGGAGCAGG TGCTGCTGTG TGCGTCCAAT CAGAGCGGCA GCATCGTGGA GTGCTGGTCG 
CTGAGGAAGG AGGGACTTCC TGTTAATAAC ATCTTCCAGC ACCGCTCTCC AGTTGTGGGC 
GAGAAGCAGC CGACCATCCT GAAGTGGCGC ATCCTGACGA CCACCAGCGA CCTGGAGCGC 
GTTTCCACCA TGGCTCTACC CAAACTGCCC ATCTCCACCT CCAACACCGA CCTGAAGGTG 
GCCTCAGACA CCAAGTTCTT TCCTGGGCTC GGTCTGGCTC TGGCCTTCCA CGACGGCAGC 
ATCCAGATCC TCCATCGCCT CTCCCTCCAC ACCATGGCAG TCTTCTATGG CTCCTCCTCC 
GCCCAGCGGC CTGGAGACGA GGCAGCTATC AAACGGCAGA GGACCGCAGG TCCCGCCCTC 
CATTTCAAGG CCCTGCAGTT ATCCTGGACC TCATTGGCCC TAGCTGGAGT TGACAGCCAC 
GGAAAGCTCC ACATGCTGCG AGTGTCGCCC TCTATGGGCC AAGTGTTGGA CATGAACACC 
ACACTGCGCC ACCTGCTGTT TCTGCTAGAG TACTGCATGG TGACAGGCTA TGACTGGTGG 
GACGTTCTGC TGCACGTTCA GCCCACTATG GTCCACAACC TGGTGGAGAA GCTGCACGAG 
GAGTACATGA GGCAGAACCA GGCGCTACAG CAGGTTCTGG CGACACGCAT CGTAGCCGTC 
AAGGCCTCCC TCTGTAAACT CTCCACGGCA ACGGCTGCGC GAGCGTGTGA CTTTCACGCC 
AAACTGCTGC TGATCGCCAT CAGCTCCACC TTAAAGTCTC TGCTCAGACC TCACGTCCTC 
AACACGCCCG ACAAGAGTCC AGGTGACCGA CTGACGGAGA TCTGCGCCAA GAACACAGAC 
ACAGACATTG ATAAGGTGAT GATCAATCTG AAGACGGAGG AGTTTGTTCT GGATGGACCT 
CCTCTGCAGT CGTTGCAGCA GCTCATCCAG TGGGTGGGAG ACTTTGTCCT GTACCTGCTG 
GCAAACCTGC CCAACCAGGT GAGCCAGGGC TCCATTGTGC GGCCCGGTTT TGGCTTCATG 
AGAGACGGGG CATCTTTGGG GATGCTGAGG GAGATGCTGG TGATGATCCG GATCTGGGGG 
CTGCTGAAGC CCGGCTGCCT CCCCACTTTC ACGGCCATGT CAGACAACCA GGACAGCATG 
CAGCTGCTGT TCAGACTGCT CACCAAGCTG TGGCTGTGCT CTCGGGAGGA TGGCACCTCC 
CAGGAACCTG ACGAGAGCCT AATAGACGAA TGTTGCCTTC TGCCCAGCCA GCTGCTGGTT 
CCCAGTATGG ACTGGCTCCC AGTGAACGAT GGGATCATTG TGAAGCTGCA GGGTAAGCTC 
CCCCTCCGGC TGCAGTTTGG AAGAGCGTCA TCGCTGCAGG TAGTGGGAGG CACCGCCCCC 
CTGGACGTCT TCACCAGGAG TCCCGGAGCT CAGAAGATGG ACAATCTGCG CTGCATCCAC 
ATGGGCGTCA GTCCCACCGA GGAGAGTAAA GTGTGCACCA GGTGCGGCTG TGTGACGATG 
CTGCGATCTC CCAACAAGAC GAACGCCATG AAGCAGTGGG AGCAGCGCTG GATCAAGAAC 
TGCCTCTGTG GGGGTCTGTG GAGGAGGATC CCGCCAACGT TCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med16 ( XM_015399004.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015254490.1 Cyprinodon variegatus mediator complex subunit 16 (med16), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCy27062
Clone ID Related Accession (Same CDS sequence)	XM_015399004.1
Accession Version	XM_015399004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	mediator of RNA polymerase II transcription subunit 16 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015151433.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG CTTATGTGTG TGAGTGGGAG AAACGTCCAA AAAGCACCCA CTGCCCATCC 
ATCCCTCTGG TCTGCTCCTG GTCCTGCAGG AACCTGATAG CCTTCACCAC AGACCTGAAG 
AATGACGACG ATGACAAAGA TGTCAGTCAC ATGATCCACA TCATCGACAC CGAGCACCCC 
TGGGACATGT TCTCCATCAA CTCTGGCCAC AGTGAGGTCA TTTCCTGTCT GGAGTGGGAC 
CAGTCAGGTT CTCGCCTGCT GTCCGCTGAT GGTGACGGTC AGATTAAGTG CTGGTCCATG 
TCGGACCACC TGGTCAACAG CTGGGAGTGC GCCTTCTCCA GCTCTGTGGA TGGAGATCCC 
ATCGTGGCTC TGAGCTGGCT GCACAACGGT GTCAAACTGG CCCTGCATGT CGAGATGTCC 
GGCTCCACAA ACTTCGGGGA GAAGTTTTCC AGGGTAAAGT TCTCTCCGTC TATGACTCTT 
TTCGGCGGGA AGCCGATGGA GGGCTGGTTG GCGGTGACGG TGAGCGGTCT GGTCAGCGTG 
TCGCTGCTGA AGCCGGGCGG CGCTCTGCTG ACGGCCAGCG AGAGCCTGTG CCGGCTGAGA 
GGCCGCGTGG CCTCGGCCGA CATCGCCTTC ACCGGCGGCG GGAACATCGT GGTGGCGGCC 
ACCGATGGCA GCAGCTCCTC CCCGGTCCAG TTCTACAAGG TGCAGGTGAG CGTTGTGAGC 
GAGAAATGTC GCATCGACAC CGAGCTGCTG CCATCGCTGT TCCTGCGCTG CACCACCGAC 
CCCATGAGGA GGGACAAGTA CCCAGCCGTC ACCCACCTCA AGTTCCTGAC CCGGGAGAAC 
TCGGAGCAGG TGCTGCTGTG TGCGTCCAAT CAGAGCGGCA GCATCGTGGA GTGCTGGTCG 
CTGAGGAAGG AGGGACTTCC TGTTAATAAC ATCTTCCAGC ACCGCTCTCC AGTTGTGGGC 
GAGAAGCAGC CGACCATCCT GAAGTGGCGC ATCCTGACGA CCACCAGCGA CCTGGAGCGC 
GTTTCCACCA TGGCTCTACC CAAACTGCCC ATCTCCACCT CCAACACCGA CCTGAAGGTG 
GCCTCAGACA CCAAGTTCTT TCCTGGGCTC GGTCTGGCTC TGGCCTTCCA CGACGGCAGC 
ATCCAGATCC TCCATCGCCT CTCCCTCCAC ACCATGGCAG TCTTCTATGG CTCCTCCTCC 
GCCCAGCGGC CTGGAGACGA GGCAGCTATC AAACGGCAGA GGACCGCAGG TCCCGCCCTC 
CATTTCAAGG CCCTGCAGTT ATCCTGGACC TCATTGGCCC TAGCTGGAGT TGACAGCCAC 
GGAAAGCTCC ACATGCTGCG AGTGTCGCCC TCTATGGGCC AAGTGTTGGA CATGAACACC 
ACACTGCGCC ACCTGCTGTT TCTGCTAGAG TACTGCATGG TGACAGGCTA TGACTGGTGG 
GACGTTCTGC TGCACGTTCA GCCCACTATG GTCCACAACC TGGTGGAGAA GCTGCACGAG 
GAGTACATGA GGCAGAACCA GGCGCTACAG CAGGTTCTGG CGACACGCAT CGTAGCCGTC 
AAGGCCTCCC TCTGTAAACT CTCCACGGCA ACGGCTGCGC GAGCGTGTGA CTTTCACGCC 
AAACTGCTGC TGATCGCCAT CAGCTCCACC TTAAAGTCTC TGCTCAGACC TCACGTCCTC 
AACACGCCCG ACAAGAGTCC AGGTGACCGA CTGACGGAGA TCTGCGCCAA GAACACAGAC 
ACAGACATTG ATAAGGTGAT GATCAATCTG AAGACGGAGG AGTTTGTTCT GGATGGACCT 
CCTCTGCAGT CGTTGCAGCA GCTCATCCAG TGGGTGGGAG ACTTTGTCCT GTACCTGCTG 
GCAAACCTGC CCAACCAGGG CTCCATTGTG CGGCCCGGTT TTGGCTTCAT GAGAGACGGG 
GCATCTTTGG GGATGCTGAG GGAGATGCTG GTGATGATCC GGATCTGGGG GCTGCTGAAG 
CCCGGCTGCC TCCCCACTTT CACGGCCATG TCAGACAACC AGGACAGCAT GCAGCTGCTG 
TTCAGACTGC TCACCAAGCT GTGGCTGTGC TCTCGGGAGG ATGGCACCTC CCAGGAACCT 
GACGAGAGCC TAATAGACGA ATGTTGCCTT CTGCCCAGCC AGCTGCTGGT TCCCAGTATG 
GACTGGCTCC CAGTGAACGA TGGGATCATT GTGAAGCTGC AGGGTAAGCT CCCCCTCCGG 
CTGCAGTTTG GAAGAGCGTC ATCGCTGCAG GTAGTGGGAG GCACCGCCCC CCTGGACGTC 
TTCACCAGGA GTCCCGGAGC TCAGAAGATG GACAATCTGC GCTGCATCCA CATGGGCGTC 
AGTCCCACCG AGGAGAGTAA AGTGTGCACC AGGTGCGGCT GTGTGACGAT GCTGCGATCT 
CCCAACAAGA CGAACGCCAT GAAGCAGTGG GAGCAGCGCT GGATCAAGAA CTGCCTCTGT 
GGGGGTCTGT GGAGGAGGAT CCCGCCAACG TTCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015254490.1
CDS	396..2894
Translation	MELAYVCEWEKRPKSTHCPSIPLVCSWSCRNLIAFTTDLKNDDDDKDVSHMIHIIDTEHPWDMFSINSGHSEVISCLEWDQSGSRLLSADGDGQIKCWSMSDHLVNSWECAFSSSVDGDPIVALSWLHNGVKLALHVEMSGSTNFGEKFSRVKFSPSMTLFGGKPMEGWLAVTVSGLVSVSLLKPGGALLTASESLCRLRGRVASADIAFTGGGNIVVAATDGSSSSPVQFYKVQVSVVSEKCRIDTELLPSLFLRCTTDPMRRDKYPAVTHLKFLTRENSEQVLLCASNQSGSIVECWSLRKEGLPVNNIFQHRSPVVGEKQPTILKWRILTTTSDLERVSTMALPKLPISTSNTDLKVASDTKFFPGLGLALAFHDGSIQILHRLSLHTMAVFYGSSSAQRPGDEAAIKRQRTAGPALHFKALQLSWTSLALAGVDSHGKLHMLRVSPSMGQVLDMNTTLRHLLFLLEYCMVTGYDWWDVLLHVQPTMVHNLVEKLHEEYMRQNQALQQVLATRIVAVKASLCKLSTATAARACDFHAKLLLIAISSTLKSLLRPHVLNTPDKSPGDRLTEICAKNTDTDIDKVMINLKTEEFVLDGPPLQSLQQLIQWVGDFVLYLLANLPNQGSIVRPGFGFMRDGASLGMLREMLVMIRIWGLLKPGCLPTFTAMSDNQDSMQLLFRLLTKLWLCSREDGTSQEPDESLIDECCLLPSQLLVPSMDWLPVNDGIIVKLQGKLPLRLQFGRASSLQVVGGTAPLDVFTRSPGAQKMDNLRCIHMGVSPTEESKVCTRCGCVTMLRSPNKTNAMKQWEQRWIKNCLCGGLWRRIPPTFS

Target ORF information:

RefSeq Version	XM_015399004.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus mediator complex subunit 16 (med16), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015399004.1

ORF Insert Sequence:

ATGGAACTGG CTTATGTGTG TGAGTGGGAG AAACGTCCAA AAAGCACCCA CTGCCCATCC 
ATCCCTCTGG TCTGCTCCTG GTCCTGCAGG AACCTGATAG CCTTCACCAC AGACCTGAAG 
AATGACGACG ATGACAAAGA TGTCAGTCAC ATGATCCACA TCATCGACAC CGAGCACCCC 
TGGGACATGT TCTCCATCAA CTCTGGCCAC AGTGAGGTCA TTTCCTGTCT GGAGTGGGAC 
CAGTCAGGTT CTCGCCTGCT GTCCGCTGAT GGTGACGGTC AGATTAAGTG CTGGTCCATG 
TCGGACCACC TGGTCAACAG CTGGGAGTGC GCCTTCTCCA GCTCTGTGGA TGGAGATCCC 
ATCGTGGCTC TGAGCTGGCT GCACAACGGT GTCAAACTGG CCCTGCATGT CGAGATGTCC 
GGCTCCACAA ACTTCGGGGA GAAGTTTTCC AGGGTAAAGT TCTCTCCGTC TATGACTCTT 
TTCGGCGGGA AGCCGATGGA GGGCTGGTTG GCGGTGACGG TGAGCGGTCT GGTCAGCGTG 
TCGCTGCTGA AGCCGGGCGG CGCTCTGCTG ACGGCCAGCG AGAGCCTGTG CCGGCTGAGA 
GGCCGCGTGG CCTCGGCCGA CATCGCCTTC ACCGGCGGCG GGAACATCGT GGTGGCGGCC 
ACCGATGGCA GCAGCTCCTC CCCGGTCCAG TTCTACAAGG TGCAGGTGAG CGTTGTGAGC 
GAGAAATGTC GCATCGACAC CGAGCTGCTG CCATCGCTGT TCCTGCGCTG CACCACCGAC 
CCCATGAGGA GGGACAAGTA CCCAGCCGTC ACCCACCTCA AGTTCCTGAC CCGGGAGAAC 
TCGGAGCAGG TGCTGCTGTG TGCGTCCAAT CAGAGCGGCA GCATCGTGGA GTGCTGGTCG 
CTGAGGAAGG AGGGACTTCC TGTTAATAAC ATCTTCCAGC ACCGCTCTCC AGTTGTGGGC 
GAGAAGCAGC CGACCATCCT GAAGTGGCGC ATCCTGACGA CCACCAGCGA CCTGGAGCGC 
GTTTCCACCA TGGCTCTACC CAAACTGCCC ATCTCCACCT CCAACACCGA CCTGAAGGTG 
GCCTCAGACA CCAAGTTCTT TCCTGGGCTC GGTCTGGCTC TGGCCTTCCA CGACGGCAGC 
ATCCAGATCC TCCATCGCCT CTCCCTCCAC ACCATGGCAG TCTTCTATGG CTCCTCCTCC 
GCCCAGCGGC CTGGAGACGA GGCAGCTATC AAACGGCAGA GGACCGCAGG TCCCGCCCTC 
CATTTCAAGG CCCTGCAGTT ATCCTGGACC TCATTGGCCC TAGCTGGAGT TGACAGCCAC 
GGAAAGCTCC ACATGCTGCG AGTGTCGCCC TCTATGGGCC AAGTGTTGGA CATGAACACC 
ACACTGCGCC ACCTGCTGTT TCTGCTAGAG TACTGCATGG TGACAGGCTA TGACTGGTGG 
GACGTTCTGC TGCACGTTCA GCCCACTATG GTCCACAACC TGGTGGAGAA GCTGCACGAG 
GAGTACATGA GGCAGAACCA GGCGCTACAG CAGGTTCTGG CGACACGCAT CGTAGCCGTC 
AAGGCCTCCC TCTGTAAACT CTCCACGGCA ACGGCTGCGC GAGCGTGTGA CTTTCACGCC 
AAACTGCTGC TGATCGCCAT CAGCTCCACC TTAAAGTCTC TGCTCAGACC TCACGTCCTC 
AACACGCCCG ACAAGAGTCC AGGTGACCGA CTGACGGAGA TCTGCGCCAA GAACACAGAC 
ACAGACATTG ATAAGGTGAT GATCAATCTG AAGACGGAGG AGTTTGTTCT GGATGGACCT 
CCTCTGCAGT CGTTGCAGCA GCTCATCCAG TGGGTGGGAG ACTTTGTCCT GTACCTGCTG 
GCAAACCTGC CCAACCAGGG CTCCATTGTG CGGCCCGGTT TTGGCTTCAT GAGAGACGGG 
GCATCTTTGG GGATGCTGAG GGAGATGCTG GTGATGATCC GGATCTGGGG GCTGCTGAAG 
CCCGGCTGCC TCCCCACTTT CACGGCCATG TCAGACAACC AGGACAGCAT GCAGCTGCTG 
TTCAGACTGC TCACCAAGCT GTGGCTGTGC TCTCGGGAGG ATGGCACCTC CCAGGAACCT 
GACGAGAGCC TAATAGACGA ATGTTGCCTT CTGCCCAGCC AGCTGCTGGT TCCCAGTATG 
GACTGGCTCC CAGTGAACGA TGGGATCATT GTGAAGCTGC AGGGTAAGCT CCCCCTCCGG 
CTGCAGTTTG GAAGAGCGTC ATCGCTGCAG GTAGTGGGAG GCACCGCCCC CCTGGACGTC 
TTCACCAGGA GTCCCGGAGC TCAGAAGATG GACAATCTGC GCTGCATCCA CATGGGCGTC 
AGTCCCACCG AGGAGAGTAA AGTGTGCACC AGGTGCGGCT GTGTGACGAT GCTGCGATCT 
CCCAACAAGA CGAACGCCAT GAAGCAGTGG GAGCAGCGCT GGATCAAGAA CTGCCTCTGT 
GGGGGTCTGT GGAGGAGGAT CCCGCCAACG TTCTCATGA