kiaa0825 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene
Clones
gRNAs

Summary

Gene Symbol	kiaa0825
Entrez Gene ID	103398217
Full Name	KIAA0825 ortholog
Gene Type	protein-coding
Organism	Cynoglossus semilaevis(tongue sole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017030643.1	XP_016886132.1	uncharacterized protein KIAA0825 homolog isoform X1
XM_008336746.3	XP_008334968.1	uncharacterized protein KIAA0825 homolog isoform X1
XM_025053022.1	XP_024908790.1	uncharacterized protein KIAA0825 homolog isoform X2
XM_025053023.1	XP_024908791.1	uncharacterized protein KIAA0825 homolog isoform X3
XM_008336746.2	XP_008334968.1	uncharacterized protein KIAA0825 homolog isoform X1
XM_017030644.1	XP_016886133.1	uncharacterized protein KIAA0825 homolog isoform X2
XM_017030644.2	XP_016886133.1	uncharacterized protein KIAA0825 homolog isoform X4
XM_017030645.1	XP_016886134.1	uncharacterized protein KIAA0825 homolog isoform X3

Pathways from BioSystems
Gene Ontology

The following kiaa0825 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kiaa0825 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCy202838	XM_025053022.1 Latest version!	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCy202839	XM_025053023.1 Latest version!	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCy63047	XM_017030643.1 Latest version!	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCy63047	XM_008336746.3 Latest version!	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCy63048	XM_017030644.2 Latest version!	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCy63049	XM_017030645.1 Latest version!	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OCy63047	XM_008336746.2	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCy63048	XM_017030644.1	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kiaa0825 ( XM_025053022.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_024908790.1 Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCy202838
Clone ID Related Accession (Same CDS sequence)	XM_025053022.1
Accession Version	XM_025053022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	uncharacterized protein KIAA0825 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024328.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGGTGTCAGA GAAGGTCCTG TGACCCAGAG 
GATAGGGGGC AACCTGGTAT GAGAGATGGA GTTCTGTGTG TCACAGGGCA TCTTTTCCAC 
TCCATCATAA AGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024908790.1
CDS	557..4414
Translation	MEWPGDFPEDHAFVEVLLPNLSIDKDYEQLLKDTEEKLNQNANSIEQQLKEIQTQMGDSPLSPAECLQWFNLEMQNSIRPLTTENQECMDFLRALQQYLKSEDDRKEELTLHLLLTLSAQCGVCFPCTSSSSLSSSPINSLHAVRDDTSVEMQDLWDDVRLQLRCHLLIRLSSRSSEQPVPSKILSLSVSESVHCLQLLFFLYPEFEVLTHYQVLRSQSVQAILHSSVSSSPGETGFDRLALGLSSTIPVLTQALTEELQVLSGLVEPHTILAVLNVAYFRTVAQELSSLMEKEFEAALRDnullnullnullTTLSSKIKKYSARSRATVAPLELSIKNRSFSLTSHQLRALTKLACTLLGYESNVKDLIANMTFINCTGDITCVKGILKTNREHSDIPKDGHKFSAGNLLHSPKVLEFDWRSAFKGLVPQMEHCVKVVLDDVCAKSLQQEEALYSAGHTSIDLCSTRGHCSTTHTKEDSFYTYSEDTPKMIAKFCGSVLSELDALLPLALVCKDSSLLEVRSSFLEACGRAAFAMLGRLQDRALAVASSAPLKNLPALLATCIYVHQRLEHYHARLKDANVVVGKIPLTLLPIQKYQEMTDALREQLSSYCVQICCTCIFQDAESHHWADPKPFYEGERCSFSVQMWYYFLCGLRSDLWAVLPADVAKDVLGQVLSETLQLLVQRYARARPSYKRHLQIRADITLILLYLENLMWSLCERPEDLVRTKPYAAIAKIAGASDWPYQIHSLCEHLLTVLVIATAPLPLLYKTFGRKPENESDPVQPDSSVLRWLKFINSELFTERVIRDGLVGQPVLACQVRLLTSGPGCNSRLLLQMLLRGDCHLPRILLENSYFCQESSSETSTESCKAGDDFIVALFNIFNRIKNIPKALTLALQPYLERAHIWDNLYNLTDTAQRQPVLINCIQDIVAKSTNGLLKHLVSMVTEWQTTEEPSRALFKRNVPGSILAKIPKDWNYAPPESGAKGSAIKTVISLAIQAMSFILTNLPLAVASVTLPIRYLFQEAEKHLSQHSRQLRLVGLLLRTLLGFLIQRLEEADTLEEISGLVLDRGAKDCLTLLAECLQAAVGIQYSKGVPKPAVLKVLQALDENRPKWTNMQLQKARKLCSDSVFERERESAAAELTEQKIGLMLLEVCHKAGGSDYLRQIYHIIQGNEELLLSKLNGNPDSTTDAPKSVNFDFGHDSSAEALHFNPLCQFDHIGKKKLDQSVVVDWAWDWSRLLPAYQGMSQVTFKTLLANRCQRRSCDPEDRGQPGMRDGVLCVTGHLFHSIIKT

Target ORF information:

RefSeq Version	XM_025053022.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025053022.1

ORF Insert Sequence:

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGGTGTCAGA GAAGGTCCTG TGACCCAGAG 
GATAGGGGGC AACCTGGTAT GAGAGATGGA GTTCTGTGTG TCACAGGGCA TCTTTTCCAC 
TCCATCATAA AGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa0825 ( XM_025053023.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_024908791.1 Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCy202839
Clone ID Related Accession (Same CDS sequence)	XM_025053023.1
Accession Version	XM_025053023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	uncharacterized protein KIAA0825 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024328.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GAGTGTGGTG GTCGACTGGG CGTGGGACTG GTCGAGGCTA 
CTGCCAGCCT ACCAGGGCAT GAGTCAGGTC ACCTTCAAAA CTCTGCTGGC AAACAGGTGG 
GAGATGCAGA AGGACGCAGA GCTGGAAGAT GGAGAGAAAA CTATGGTAGA AGAACTCAAA 
AAAGCCTATT TTGTTTGCTG CTGCTGCAGC AGCAGTCTGG GAGTCCAGGA CCCTCCTGAA 
GCTGATGAGG CACCAGTGAA GCAGACCCAG GGGGAAACAC AAGACTCTCA TGGAAGTGTG 
GCCCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024908791.1
CDS	557..4345
Translation	MEWPGDFPEDHAFVEVLLPNLSIDKDYEQLLKDTEEKLNQNANSIEQQLKEIQTQMGDSPLSPAECLQWFNLEMQNSIRPLTTENQECMDFLRALQQYLKSEDDRKEELTLHLLLTLSAQCGVCFPCTSSSSLSSSPINSLHAVRDDTSVEMQDLWDDVRLQLRCHLLIRLSSRSSEQPVPSKILSLSVSESVHCLQLLFFLYPEFEVLTHYQVLRSQSVQAILHSSVSSSPGETGFDRLALGLSSTIPVLTQALTEELQVLSGLVEPHTILAVLNVAYFRTVAQELSSLMEKEFEAALRDnullnullnullTTLSSKIKKYSARSRATVAPLELSIKNRSFSLTSHQLRALTKLACTLLGYESNVKDLIANMTFINCTGDITCVKGILKTNREHSDIPKDGHKFSAGNLLHSPKVLEFDWRSAFKGLVPQMEHCVKVVLDDVCAKSLQQEEALYSAGHTSIDLCSTRGHCSTTHTKEDSFYTYSEDTPKMIAKFCGSVLSELDALLPLALVCKDSSLLEVRSSFLEACGRAAFAMLGRLQDRALAVASSAPLKNLPALLATCIYVHQRLEHYHARLKDANVVVGKIPLTLLPIQKYQEMTDALREQLSSYCVQICCTCIFQDAESHHWADPKPFYEGERCSFSVQMWYYFLCGLRSDLWAVLPADVAKDVLGQVLSETLQLLVQRYARARPSYKRHLQIRADITLILLYLENLMWSLCERPEDLVRTKPYAAIAKIAGASDWPYQIHSLCEHLLTVLVIATAPLPLLYKTFGRKPENESDPVQPDSSVLRWLKFINSELFTERVIRDGLVGQPVLACQVRLLTSGPGCNSRLLLQMLLRGDCHLPRILLENSYFCQESSSETSTESCKAGDDFIVALFNIFNRIKNIPKALTLALQPYLERAHIWDNLYNLTDTAQRQPVLINCIQDIVAKSTNGLLKHLVSMVTEWQTTEEPSRALFKRNVPGSILAKIPKDWNYAPPESGAKGSAIKTVISLAIQAMSFILTNLPLAVASVTLPIRYLFQEAEKHLSQHSRQLRLVGLLLRTLLGFLIQRLEEADTLEEISGLVLDRGAKDCLTLLAECLQAAVGIQYSKGVPKPAVLKVLQALDENRPKWTNMQLQKARKLCSDSVFERERESAAAELTEQKIGLMLLEVCHKAGGSDYLRQIYHIIQGNESVVVDWAWDWSRLLPAYQGMSQVTFKTLLANRWEMQKDAELEDGEKTMVEELKKAYFVCCCCSSSLGVQDPPEADEAPVKQTQGETQDSHGSVAR

Target ORF information:

RefSeq Version	XM_025053023.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025053023.1

ORF Insert Sequence:

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GAGTGTGGTG GTCGACTGGG CGTGGGACTG GTCGAGGCTA 
CTGCCAGCCT ACCAGGGCAT GAGTCAGGTC ACCTTCAAAA CTCTGCTGGC AAACAGGTGG 
GAGATGCAGA AGGACGCAGA GCTGGAAGAT GGAGAGAAAA CTATGGTAGA AGAACTCAAA 
AAAGCCTATT TTGTTTGCTG CTGCTGCAGC AGCAGTCTGG GAGTCCAGGA CCCTCCTGAA 
GCTGATGAGG CACCAGTGAA GCAGACCCAG GGGGAAACAC AAGACTCTCA TGGAAGTGTG 
GCCCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa0825 ( XM_017030643.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_016886132.1 Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X2, mRNA.

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CloneID	OCy63047
Clone ID Related Accession (Same CDS sequence)	XM_017030643.1 , XM_008336746.3 , XM_008336746.2
Accession Version	XM_017030643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	uncharacterized protein KIAA0825 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024328.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGGTGGGAGA TGCAGAAGGA CGCAGAGCTG 
GAAGATGGAG AGAAAACTAT GGTAGAAGAA CTCAAAAAAG CCTATTTTGT TTGCTGCTGC 
TGCAGCAGCA GTCTGGGAGT CCAGGACCCT CCTGAAGCTG ATGAGGCACC AGTGAAGCAG 
ACCCAGGGGG AAACACAAGA CTCTCATGGA AGTGTGGCCC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016886132.1
CDS	328..4272
Translation	MEWPGDFPEDHAFVEVLLPNLSIDKDYEQLLKDTEEKLNQNANSIEQQLKEIQTQMGDSPLSPAECLQWFNLEMQNSIRPLTTENQECMDFLRALQQYLKSEDDRKEELTLHLLLTLSAQCGVCFPCTSSSSLSSSPINSLHAVRDDTSVEMQDLWDDVRLQLRCHLLIRLSSRSSEQPVPSKILSLSVSESVHCLQLLFFLYPEFEVLTHYQVLRSQSVQAILHSSVSSSPGETGFDRLALGLSSTIPVLTQALTEELQVLSGLVEPHTILAVLNVAYFRTVAQELSSLMEKEFEAALRDnullnullnullTTLSSKIKKYSARSRATVAPLELSIKNRSFSLTSHQLRALTKLACTLLGYESNVKDLIANMTFINCTGDITCVKGILKTNREHSDIPKDGHKFSAGNLLHSPKVLEFDWRSAFKGLVPQMEHCVKVVLDDVCAKSLQQEEALYSAGHTSIDLCSTRGHCSTTHTKEDSFYTYSEDTPKMIAKFCGSVLSELDALLPLALVCKDSSLLEVRSSFLEACGRAAFAMLGRLQDRALAVASSAPLKNLPALLATCIYVHQRLEHYHARLKDANVVVGKIPLTLLPIQKYQEMTDALREQLSSYCVQICCTCIFQDAESHHWADPKPFYEGERCSFSVQMWYYFLCGLRSDLWAVLPADVAKDVLGQVLSETLQLLVQRYARARPSYKRHLQIRADITLILLYLENLMWSLCERPEDLVRTKPYAAIAKIAGASDWPYQIHSLCEHLLTVLVIATAPLPLLYKTFGRKPENESDPVQPDSSVLRWLKFINSELFTERVIRDGLVGQPVLACQVRLLTSGPGCNSRLLLQMLLRGDCHLPRILLENSYFCQESSSETSTESCKAGDDFIVALFNIFNRIKNIPKALTLALQPYLERAHIWDNLYNLTDTAQRQPVLINCIQDIVAKSTNGLLKHLVSMVTEWQTTEEPSRALFKRNVPGSILAKIPKDWNYAPPESGAKGSAIKTVISLAIQAMSFILTNLPLAVASVTLPIRYLFQEAEKHLSQHSRQLRLVGLLLRTLLGFLIQRLEEADTLEEISGLVLDRGAKDCLTLLAECLQAAVGIQYSKGVPKPAVLKVLQALDENRPKWTNMQLQKARKLCSDSVFERERESAAAELTEQKIGLMLLEVCHKAGGSDYLRQIYHIIQGNEELLLSKLNGNPDSTTDAPKSVNFDFGHDSSAEALHFNPLCQFDHIGKKKLDQSVVVDWAWDWSRLLPAYQGMSQVTFKTLLANRWEMQKDAELEDGEKTMVEELKKAYFVCCCCSSSLGVQDPPEADEAPVKQTQGETQDSHGSVAR

Target ORF information:

RefSeq Version	XM_017030643.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017030643.1

ORF Insert Sequence:

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGGTGGGAGA TGCAGAAGGA CGCAGAGCTG 
GAAGATGGAG AGAAAACTAT GGTAGAAGAA CTCAAAAAAG CCTATTTTGT TTGCTGCTGC 
TGCAGCAGCA GTCTGGGAGT CCAGGACCCT CCTGAAGCTG ATGAGGCACC AGTGAAGCAG 
ACCCAGGGGG AAACACAAGA CTCTCATGGA AGTGTGGCCC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa0825 ( XM_008336746.3 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008334968.1 Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCy63047
Clone ID Related Accession (Same CDS sequence)	XM_017030643.1 , XM_008336746.3 , XM_008336746.2
Accession Version	XM_008336746.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	uncharacterized protein KIAA0825 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024328.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_008336746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGGTGGGAGA TGCAGAAGGA CGCAGAGCTG 
GAAGATGGAG AGAAAACTAT GGTAGAAGAA CTCAAAAAAG CCTATTTTGT TTGCTGCTGC 
TGCAGCAGCA GTCTGGGAGT CCAGGACCCT CCTGAAGCTG ATGAGGCACC AGTGAAGCAG 
ACCCAGGGGG AAACACAAGA CTCTCATGGA AGTGTGGCCC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008334968.1
CDS	557..4501
Translation	MEWPGDFPEDHAFVEVLLPNLSIDKDYEQLLKDTEEKLNQNANSIEQQLKEIQTQMGDSPLSPAECLQWFNLEMQNSIRPLTTENQECMDFLRALQQYLKSEDDRKEELTLHLLLTLSAQCGVCFPCTSSSSLSSSPINSLHAVRDDTSVEMQDLWDDVRLQLRCHLLIRLSSRSSEQPVPSKILSLSVSESVHCLQLLFFLYPEFEVLTHYQVLRSQSVQAILHSSVSSSPGETGFDRLALGLSSTIPVLTQALTEELQVLSGLVEPHTILAVLNVAYFRTVAQELSSLMEKEFEAALRDnullnullnullTTLSSKIKKYSARSRATVAPLELSIKNRSFSLTSHQLRALTKLACTLLGYESNVKDLIANMTFINCTGDITCVKGILKTNREHSDIPKDGHKFSAGNLLHSPKVLEFDWRSAFKGLVPQMEHCVKVVLDDVCAKSLQQEEALYSAGHTSIDLCSTRGHCSTTHTKEDSFYTYSEDTPKMIAKFCGSVLSELDALLPLALVCKDSSLLEVRSSFLEACGRAAFAMLGRLQDRALAVASSAPLKNLPALLATCIYVHQRLEHYHARLKDANVVVGKIPLTLLPIQKYQEMTDALREQLSSYCVQICCTCIFQDAESHHWADPKPFYEGERCSFSVQMWYYFLCGLRSDLWAVLPADVAKDVLGQVLSETLQLLVQRYARARPSYKRHLQIRADITLILLYLENLMWSLCERPEDLVRTKPYAAIAKIAGASDWPYQIHSLCEHLLTVLVIATAPLPLLYKTFGRKPENESDPVQPDSSVLRWLKFINSELFTERVIRDGLVGQPVLACQVRLLTSGPGCNSRLLLQMLLRGDCHLPRILLENSYFCQESSSETSTESCKAGDDFIVALFNIFNRIKNIPKALTLALQPYLERAHIWDNLYNLTDTAQRQPVLINCIQDIVAKSTNGLLKHLVSMVTEWQTTEEPSRALFKRNVPGSILAKIPKDWNYAPPESGAKGSAIKTVISLAIQAMSFILTNLPLAVASVTLPIRYLFQEAEKHLSQHSRQLRLVGLLLRTLLGFLIQRLEEADTLEEISGLVLDRGAKDCLTLLAECLQAAVGIQYSKGVPKPAVLKVLQALDENRPKWTNMQLQKARKLCSDSVFERERESAAAELTEQKIGLMLLEVCHKAGGSDYLRQIYHIIQGNEELLLSKLNGNPDSTTDAPKSVNFDFGHDSSAEALHFNPLCQFDHIGKKKLDQSVVVDWAWDWSRLLPAYQGMSQVTFKTLLANRWEMQKDAELEDGEKTMVEELKKAYFVCCCCSSSLGVQDPPEADEAPVKQTQGETQDSHGSVAR

Target ORF information:

RefSeq Version	XM_008336746.3
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008336746.3

ORF Insert Sequence:

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGGTGGGAGA TGCAGAAGGA CGCAGAGCTG 
GAAGATGGAG AGAAAACTAT GGTAGAAGAA CTCAAAAAAG CCTATTTTGT TTGCTGCTGC 
TGCAGCAGCA GTCTGGGAGT CCAGGACCCT CCTGAAGCTG ATGAGGCACC AGTGAAGCAG 
ACCCAGGGGG AAACACAAGA CTCTCATGGA AGTGTGGCCC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa0825 ( XM_008336746.2 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008334968.1 Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCy63047
Clone ID Related Accession (Same CDS sequence)	XM_017030643.1 , XM_008336746.3 , XM_008336746.2
Accession Version	XM_008336746.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	uncharacterized protein KIAA0825 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024328.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 7, 2016 this sequence version replaced XM_008336746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGGTGGGAGA TGCAGAAGGA CGCAGAGCTG 
GAAGATGGAG AGAAAACTAT GGTAGAAGAA CTCAAAAAAG CCTATTTTGT TTGCTGCTGC 
TGCAGCAGCA GTCTGGGAGT CCAGGACCCT CCTGAAGCTG ATGAGGCACC AGTGAAGCAG 
ACCCAGGGGG AAACACAAGA CTCTCATGGA AGTGTGGCCC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008334968.1
CDS	331..4275
Translation	MEWPGDFPEDHAFVEVLLPNLSIDKDYEQLLKDTEEKLNQNANSIEQQLKEIQTQMGDSPLSPAECLQWFNLEMQNSIRPLTTENQECMDFLRALQQYLKSEDDRKEELTLHLLLTLSAQCGVCFPCTSSSSLSSSPINSLHAVRDDTSVEMQDLWDDVRLQLRCHLLIRLSSRSSEQPVPSKILSLSVSESVHCLQLLFFLYPEFEVLTHYQVLRSQSVQAILHSSVSSSPGETGFDRLALGLSSTIPVLTQALTEELQVLSGLVEPHTILAVLNVAYFRTVAQELSSLMEKEFEAALRDnullnullnullTTLSSKIKKYSARSRATVAPLELSIKNRSFSLTSHQLRALTKLACTLLGYESNVKDLIANMTFINCTGDITCVKGILKTNREHSDIPKDGHKFSAGNLLHSPKVLEFDWRSAFKGLVPQMEHCVKVVLDDVCAKSLQQEEALYSAGHTSIDLCSTRGHCSTTHTKEDSFYTYSEDTPKMIAKFCGSVLSELDALLPLALVCKDSSLLEVRSSFLEACGRAAFAMLGRLQDRALAVASSAPLKNLPALLATCIYVHQRLEHYHARLKDANVVVGKIPLTLLPIQKYQEMTDALREQLSSYCVQICCTCIFQDAESHHWADPKPFYEGERCSFSVQMWYYFLCGLRSDLWAVLPADVAKDVLGQVLSETLQLLVQRYARARPSYKRHLQIRADITLILLYLENLMWSLCERPEDLVRTKPYAAIAKIAGASDWPYQIHSLCEHLLTVLVIATAPLPLLYKTFGRKPENESDPVQPDSSVLRWLKFINSELFTERVIRDGLVGQPVLACQVRLLTSGPGCNSRLLLQMLLRGDCHLPRILLENSYFCQESSSETSTESCKAGDDFIVALFNIFNRIKNIPKALTLALQPYLERAHIWDNLYNLTDTAQRQPVLINCIQDIVAKSTNGLLKHLVSMVTEWQTTEEPSRALFKRNVPGSILAKIPKDWNYAPPESGAKGSAIKTVISLAIQAMSFILTNLPLAVASVTLPIRYLFQEAEKHLSQHSRQLRLVGLLLRTLLGFLIQRLEEADTLEEISGLVLDRGAKDCLTLLAECLQAAVGIQYSKGVPKPAVLKVLQALDENRPKWTNMQLQKARKLCSDSVFERERESAAAELTEQKIGLMLLEVCHKAGGSDYLRQIYHIIQGNEELLLSKLNGNPDSTTDAPKSVNFDFGHDSSAEALHFNPLCQFDHIGKKKLDQSVVVDWAWDWSRLLPAYQGMSQVTFKTLLANRWEMQKDAELEDGEKTMVEELKKAYFVCCCCSSSLGVQDPPEADEAPVKQTQGETQDSHGSVAR

Target ORF information:

RefSeq Version	XM_008336746.2
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008336746.2

ORF Insert Sequence:

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGGTGGGAGA TGCAGAAGGA CGCAGAGCTG 
GAAGATGGAG AGAAAACTAT GGTAGAAGAA CTCAAAAAAG CCTATTTTGT TTGCTGCTGC 
TGCAGCAGCA GTCTGGGAGT CCAGGACCCT CCTGAAGCTG ATGAGGCACC AGTGAAGCAG 
ACCCAGGGGG AAACACAAGA CTCTCATGGA AGTGTGGCCC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa0825 ( XM_017030644.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_016886133.1 Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X3, mRNA.

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CloneID	OCy63048
Clone ID Related Accession (Same CDS sequence)	XM_017030644.1 , XM_017030644.2
Accession Version	XM_017030644.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	uncharacterized protein KIAA0825 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024328.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGATGTGTCA GTGCAAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016886133.1
CDS	331..4101
Translation	MEWPGDFPEDHAFVEVLLPNLSIDKDYEQLLKDTEEKLNQNANSIEQQLKEIQTQMGDSPLSPAECLQWFNLEMQNSIRPLTTENQECMDFLRALQQYLKSEDDRKEELTLHLLLTLSAQCGVCFPCTSSSSLSSSPINSLHAVRDDTSVEMQDLWDDVRLQLRCHLLIRLSSRSSEQPVPSKILSLSVSESVHCLQLLFFLYPEFEVLTHYQVLRSQSVQAILHSSVSSSPGETGFDRLALGLSSTIPVLTQALTEELQVLSGLVEPHTILAVLNVAYFRTVAQELSSLMEKEFEAALRDnullnullnullTTLSSKIKKYSARSRATVAPLELSIKNRSFSLTSHQLRALTKLACTLLGYESNVKDLIANMTFINCTGDITCVKGILKTNREHSDIPKDGHKFSAGNLLHSPKVLEFDWRSAFKGLVPQMEHCVKVVLDDVCAKSLQQEEALYSAGHTSIDLCSTRGHCSTTHTKEDSFYTYSEDTPKMIAKFCGSVLSELDALLPLALVCKDSSLLEVRSSFLEACGRAAFAMLGRLQDRALAVASSAPLKNLPALLATCIYVHQRLEHYHARLKDANVVVGKIPLTLLPIQKYQEMTDALREQLSSYCVQICCTCIFQDAESHHWADPKPFYEGERCSFSVQMWYYFLCGLRSDLWAVLPADVAKDVLGQVLSETLQLLVQRYARARPSYKRHLQIRADITLILLYLENLMWSLCERPEDLVRTKPYAAIAKIAGASDWPYQIHSLCEHLLTVLVIATAPLPLLYKTFGRKPENESDPVQPDSSVLRWLKFINSELFTERVIRDGLVGQPVLACQVRLLTSGPGCNSRLLLQMLLRGDCHLPRILLENSYFCQESSSETSTESCKAGDDFIVALFNIFNRIKNIPKALTLALQPYLERAHIWDNLYNLTDTAQRQPVLINCIQDIVAKSTNGLLKHLVSMVTEWQTTEEPSRALFKRNVPGSILAKIPKDWNYAPPESGAKGSAIKTVISLAIQAMSFILTNLPLAVASVTLPIRYLFQEAEKHLSQHSRQLRLVGLLLRTLLGFLIQRLEEADTLEEISGLVLDRGAKDCLTLLAECLQAAVGIQYSKGVPKPAVLKVLQALDENRPKWTNMQLQKARKLCSDSVFERERESAAAELTEQKIGLMLLEVCHKAGGSDYLRQIYHIIQGNEELLLSKLNGNPDSTTDAPKSVNFDFGHDSSAEALHFNPLCQFDHIGKKKLDQSVVVDWAWDWSRLLPAYQGMSQVTFKTLLANRCVSAN

Target ORF information:

RefSeq Version	XM_017030644.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017030644.1

ORF Insert Sequence:

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGATGTGTCA GTGCAAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa0825 ( XM_017030644.2 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_016886133.1 Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCy63048
Clone ID Related Accession (Same CDS sequence)	XM_017030644.1 , XM_017030644.2
Accession Version	XM_017030644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	uncharacterized protein KIAA0825 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024328.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_017030644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGATGTGTCA GTGCAAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016886133.1
CDS	557..4327
Translation	MEWPGDFPEDHAFVEVLLPNLSIDKDYEQLLKDTEEKLNQNANSIEQQLKEIQTQMGDSPLSPAECLQWFNLEMQNSIRPLTTENQECMDFLRALQQYLKSEDDRKEELTLHLLLTLSAQCGVCFPCTSSSSLSSSPINSLHAVRDDTSVEMQDLWDDVRLQLRCHLLIRLSSRSSEQPVPSKILSLSVSESVHCLQLLFFLYPEFEVLTHYQVLRSQSVQAILHSSVSSSPGETGFDRLALGLSSTIPVLTQALTEELQVLSGLVEPHTILAVLNVAYFRTVAQELSSLMEKEFEAALRDnullnullnullTTLSSKIKKYSARSRATVAPLELSIKNRSFSLTSHQLRALTKLACTLLGYESNVKDLIANMTFINCTGDITCVKGILKTNREHSDIPKDGHKFSAGNLLHSPKVLEFDWRSAFKGLVPQMEHCVKVVLDDVCAKSLQQEEALYSAGHTSIDLCSTRGHCSTTHTKEDSFYTYSEDTPKMIAKFCGSVLSELDALLPLALVCKDSSLLEVRSSFLEACGRAAFAMLGRLQDRALAVASSAPLKNLPALLATCIYVHQRLEHYHARLKDANVVVGKIPLTLLPIQKYQEMTDALREQLSSYCVQICCTCIFQDAESHHWADPKPFYEGERCSFSVQMWYYFLCGLRSDLWAVLPADVAKDVLGQVLSETLQLLVQRYARARPSYKRHLQIRADITLILLYLENLMWSLCERPEDLVRTKPYAAIAKIAGASDWPYQIHSLCEHLLTVLVIATAPLPLLYKTFGRKPENESDPVQPDSSVLRWLKFINSELFTERVIRDGLVGQPVLACQVRLLTSGPGCNSRLLLQMLLRGDCHLPRILLENSYFCQESSSETSTESCKAGDDFIVALFNIFNRIKNIPKALTLALQPYLERAHIWDNLYNLTDTAQRQPVLINCIQDIVAKSTNGLLKHLVSMVTEWQTTEEPSRALFKRNVPGSILAKIPKDWNYAPPESGAKGSAIKTVISLAIQAMSFILTNLPLAVASVTLPIRYLFQEAEKHLSQHSRQLRLVGLLLRTLLGFLIQRLEEADTLEEISGLVLDRGAKDCLTLLAECLQAAVGIQYSKGVPKPAVLKVLQALDENRPKWTNMQLQKARKLCSDSVFERERESAAAELTEQKIGLMLLEVCHKAGGSDYLRQIYHIIQGNEELLLSKLNGNPDSTTDAPKSVNFDFGHDSSAEALHFNPLCQFDHIGKKKLDQSVVVDWAWDWSRLLPAYQGMSQVTFKTLLANRCVSAN

Target ORF information:

RefSeq Version	XM_017030644.2
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017030644.2

ORF Insert Sequence:

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCGTGTTTG AACGAGAACG AGAGAGCGCT GCGGCTGAAC TGACTGAGCA GAAAATAGGC 
CTGATGCTGC TGGAGGTCTG CCACAAAGCA GGTGGAAGCG ACTACCTGAG GCAGATCTAT 
CACATCATCC AAGGCAATGA GGAGCTGTTG TTGTCCAAAC TAAATGGAAA TCCTGACTCA 
ACCACAGATG CTCCCAAATC AGTGAACTTT GACTTCGGCC ATGACAGTTC TGCAGAAGCT 
TTGCATTTCA ACCCTCTCTG CCAATTCGAT CACATTGGCA AAAAGAAGTT GGATCAGAGT 
GTGGTGGTCG ACTGGGCGTG GGACTGGTCG AGGCTACTGC CAGCCTACCA GGGCATGAGT 
CAGGTCACCT TCAAAACTCT GCTGGCAAAC AGATGTGTCA GTGCAAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa0825 ( XM_017030645.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_016886134.1 Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCy63049
Clone ID Related Accession (Same CDS sequence)	XM_017030645.1
Accession Version	XM_017030645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	uncharacterized protein KIAA0825 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024328.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCATGTTCC CAGAAATTGT GTCTCTGGTC GGTCACTTCA TCATAACAGA GTCGGGATGC 
ATCCATCCTC ATAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016886134.1
CDS	331..3768
Translation	MEWPGDFPEDHAFVEVLLPNLSIDKDYEQLLKDTEEKLNQNANSIEQQLKEIQTQMGDSPLSPAECLQWFNLEMQNSIRPLTTENQECMDFLRALQQYLKSEDDRKEELTLHLLLTLSAQCGVCFPCTSSSSLSSSPINSLHAVRDDTSVEMQDLWDDVRLQLRCHLLIRLSSRSSEQPVPSKILSLSVSESVHCLQLLFFLYPEFEVLTHYQVLRSQSVQAILHSSVSSSPGETGFDRLALGLSSTIPVLTQALTEELQVLSGLVEPHTILAVLNVAYFRTVAQELSSLMEKEFEAALRDnullnullnullTTLSSKIKKYSARSRATVAPLELSIKNRSFSLTSHQLRALTKLACTLLGYESNVKDLIANMTFINCTGDITCVKGILKTNREHSDIPKDGHKFSAGNLLHSPKVLEFDWRSAFKGLVPQMEHCVKVVLDDVCAKSLQQEEALYSAGHTSIDLCSTRGHCSTTHTKEDSFYTYSEDTPKMIAKFCGSVLSELDALLPLALVCKDSSLLEVRSSFLEACGRAAFAMLGRLQDRALAVASSAPLKNLPALLATCIYVHQRLEHYHARLKDANVVVGKIPLTLLPIQKYQEMTDALREQLSSYCVQICCTCIFQDAESHHWADPKPFYEGERCSFSVQMWYYFLCGLRSDLWAVLPADVAKDVLGQVLSETLQLLVQRYARARPSYKRHLQIRADITLILLYLENLMWSLCERPEDLVRTKPYAAIAKIAGASDWPYQIHSLCEHLLTVLVIATAPLPLLYKTFGRKPENESDPVQPDSSVLRWLKFINSELFTERVIRDGLVGQPVLACQVRLLTSGPGCNSRLLLQMLLRGDCHLPRILLENSYFCQESSSETSTESCKAGDDFIVALFNIFNRIKNIPKALTLALQPYLERAHIWDNLYNLTDTAQRQPVLINCIQDIVAKSTNGLLKHLVSMVTEWQTTEEPSRALFKRNVPGSILAKIPKDWNYAPPESGAKGSAIKTVISLAIQAMSFILTNLPLAVASVTLPIRYLFQEAEKHLSQHSRQLRLVGLLLRTLLGFLIQRLEEADTLEEISGLVLDRGAKDCLTLLAECLQAAVGIQYSKGVPKPAVLKVLQALDENRPKWTNMQLQKARKLCSDSMFPEIVSLVGHFIITESGCIHPHK

Target ORF information:

RefSeq Version	XM_017030645.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis KIAA0825 ortholog (kiaa0825), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017030645.1

ORF Insert Sequence:

ATGGAGTGGC CAGGTGACTT CCCTGAAGAC CATGCATTTG TGGAGGTGCT GCTTCCCAAT 
TTATCCATTG ACAAGGACTA TGAACAGCTT TTAAAAGACA CTGAGGAGAA ACTCAATCAA 
AATGCCAACA GCATCGAACA GCAGCTAAAG GAGATCCAAA CCCAAATGGG AGACTCCCCA 
CTGAGCCCTG CAGAGTGCCT CCAGTGGTTC AACCTGGAAA TGCAAAACAG TATAAGGCCT 
CTCACAACAG AAAACCAAGA GTGCATGGAC TTCCTCAGAG CTCTGCAACA GTACCTGAAA 
TCTGAGGACG ACAGAAAAGA GGAGTTGACA CTTCATCTAC TCCTGACTCT TTCTGCCCAG 
TGTGGAGTCT GCTTCCCCTG TACCTCATCT TCTTCCTTAT CTTCTTCTCC TATTAACTCG 
CTCCACGCAG TGAGGGATGA TACCTCAGTA GAGATGCAGG ATTTATGGGA TGACGTGCGT 
CTCCAGTTAC GCTGCCACCT TCTGATTCGG CTGTCTTCTC GCAGCTCAGA GCAACCAGTA 
CCTTCAAAAA TACTGAGTCT TTCTGTCTCT GAAAGTGTCC ACTGCCTGCA GCTGTTGTTT 
TTTCTTTATC CTGAGTTTGA GGTTCTCACA CATTATCAGG TTCTAAGAAG TCAGTCTGTG 
CAGGCTATAC TGCACTCAAG TGTGAGTTCC AGCCCCGGTG AGACTGGATT TGACAGACTG 
GCTCTTGGTT TGAGCTCCAC GATCCCTGTC CTAACGCAAG CCCTCACCGA GGAACTGCAA 
GTCCTTTCAG GCCTGGTAGA GCCCCACACC ATCCTTGCTG TGCTCAATGT GGCCTACTTC 
AGAACTGTGG CCCAAGAACT GTCCTCTCTG ATGGAGAAGG AGTTTGAGGC AGCCCTGAGG 
GATNNNNNNN NNACGACGCT CAGCAGCAAG ATAAAGAAAT ATTCTGCCAG ATCCAGAGCA 
ACTGTAGCAC CATTGGAGCT TTCCATAAAA AACAGAAGCT TCAGTCTTAC TTCCCACCAG 
TTGAGGGCTT TAACCAAACT GGCCTGCACT CTGCTTGGGT ATGAGAGCAA TGTGAAGGAT 
CTGATCGCCA ACATGACCTT CATAAACTGT ACAGGAGACA TTACCTGTGT AAAAGGTATT 
TTGAAAACGA ATAGAGAGCA TTCAGACATT CCAAAGGATG GCCATAAATT CTCAGCAGGG 
AACCTCCTGC ACTCACCAAA GGTTTTGGAG TTTGACTGGA GGTCGGCATT TAAGGGTCTG 
GTCCCTCAGA TGGAGCACTG TGTTAAAGTG GTGCTAGATG ATGTTTGTGC CAAGAGTCTG 
CAGCAAGAGG AGGCTCTGTA CTCTGCAGGA CACACCTCCA TCGACCTGTG TAGTACCAGA 
GGGCACTGCA GCACCACCCA CACCAAGGAG GACTCCTTCT ACACCTACTC TGAGGACACT 
CCAAAAATGA TTGCCAAGTT CTGTGGAAGT GTCCTGTCTG AGTTGGATGC CCTACTACCA 
TTGGCACTGG TCTGCAAGGA CAGCTCTCTC CTGGAGGTGA GATCAAGCTT CCTGGAGGCA 
TGTGGAAGGG CAGCGTTTGC CATGCTGGGC CGTCTGCAGG ACAGAGCCCT GGCTGTGGCT 
TCATCTGCAC CACTGAAGAA CCTGCCTGCA CTGCTGGCCA CCTGCATTTA TGTGCACCAA 
AGACTGGAGC ACTACCATGC ACGACTGAAA GATGCCAATG TTGTGGTAGG AAAAATACCC 
TTAACTCTAC TACCAATACA GAAGTACCAA GAAATGACAG ACGCTCTGAG AGAACAACTA 
TCCAGCTATT GTGTCCAGAT CTGCTGCACA TGCATATTTC AAGATGCAGA GAGTCATCAC 
TGGGCTGATC CCAAACCCTT TTATGAGGGC GAACGCTGCT CCTTCTCCGT GCAGATGTGG 
TACTACTTTC TGTGTGGGCT CCGCAGTGAC CTGTGGGCAG TGCTTCCAGC TGATGTGGCC 
AAAGATGTTC TTGGCCAAGT GTTATCTGAG ACTCTGCAGC TGTTGGTGCA GCGATATGCC 
AGGGCCCGTC CTTCGTACAA GAGACATCTG CAGATCAGAG CTGACATCAC GCTGATTCTT 
CTCTATTTGG AGAACCTTAT GTGGAGTTTG TGTGAACGTC CTGAAGACTT GGTGAGGACC 
AAGCCTTATG CAGCCATTGC CAAAATTGCT GGTGCATCAG ACTGGCCGTA CCAAATACAC 
AGCCTGTGTG AGCATCTCTT GACAGTCCTC GTCATCGCCA CAGCACCGCT GCCTTTGCTT 
TACAAGACAT TTGGGAGAAA ACCTGAAAAT GAATCTGATC CAGTGCAGCC TGACAGTTCT 
GTTTTACGCT GGTTGAAGTT TATCAATTCT GAGCTCTTTA CAGAGCGGGT CATACGAGAT 
GGTCTTGTAG GCCAGCCTGT CCTTGCATGC CAGGTGAGGC TGCTGACCTC TGGCCCTGGA 
TGCAATTCCA GGTTGCTTCT GCAAATGCTG CTTCGTGGAG ATTGTCACCT GCCCAGGATT 
CTCCTGGAAA ACTCTTATTT TTGCCAGGAG AGTTCCTCAG AAACATCCAC TGAAAGTTGC 
AAAGCGGGTG ATGACTTTAT TGTCGCACTG TTTAACATTT TTAATCGCAT AAAGAATATT 
CCCAAGGCCT TGACTCTGGC CCTTCAACCT TACCTGGAGA GGGCACATAT ATGGGACAAT 
CTGTACAATC TGACAGATAC CGCACAGAGG CAACCTGTGC TGATTAATTG TATACAGGAT 
ATAGTCGCCA AATCTACCAA CGGCCTCCTT AAGCACCTGG TCTCTATGGT AACGGAATGG 
CAGACCACGG AGGAGCCAAG CAGAGCTTTG TTTAAGAGGA ATGTACCTGG TAGCATCCTG 
GCTAAAATAC CGAAGGACTG GAACTACGCA CCACCAGAAT CTGGGGCAAA GGGGTCTGCC 
ATAAAAACTG TGATATCCTT AGCCATTCAA GCCATGTCTT TCATTCTCAC CAACCTGCCC 
TTGGCTGTAG CATCTGTGAC TCTCCCAATT CGATACCTAT TCCAAGAGGC AGAAAAGCAC 
CTCTCCCAGC ATTCCCGGCA GCTGCGCTTA GTGGGCCTTT TGCTCCGGAC CTTGTTAGGC 
TTCCTGATCC AGCGTTTGGA AGAGGCAGAC ACACTAGAGG AGATCAGTGG TTTAGTGCTA 
GACCGTGGGG CAAAGGACTG TCTGACTCTG CTGGCTGAGT GTCTACAGGC TGCTGTTGGC 
ATTCAATATT CAAAGGGTGT GCCTAAACCT GCAGTGCTCA AAGTTCTGCA GGCTCTAGAT 
GAAAACAGAC CAAAGTGGAC AAACATGCAG CTGCAGAAGG CCCGCAAGCT CTGCTCAGAC 
AGCATGTTCC CAGAAATTGT GTCTCTGGTC GGTCACTTCA TCATAACAGA GTCGGGATGC 
ATCCATCCTC ATAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

kiaa0825 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム