ERF1 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	ERF1
Entrez Gene ID	101222924
Full Name	ethylene-responsive transcription factor 4-like
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001280596.1	NP_001267525.1	ethylene-responsive transcription factor 4-like

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RNA-Seq improves annotation of protein-coding genes in the cucumber genome.
Li Z, Zhang Z, Yan P, Huang S, Fei Z, Lin K
BMC genomics12540(2011 Nov)

Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types.
Guo S, Zheng Y, Joung JG, Liu S, Zhang Z, Crasta OR, Sobral BW, Xu Y, Huang S, Fei Z
BMC genomics11384(2010 Jun)

The genome of the cucumber, Cucumis sativus L.
Huang S, Li R, Zhang Z, Li L, Gu X, Fan W, Lucas WJ, Wang X, Xie B, Ni P, Ren Y, Zhu H, Li J, Lin K, Jin W, Fei Z, Li G, Staub J, Kilian A, van der Vossen EA, Wu Y, Guo J, He J, Jia Z, Ren Y, Tian G, Lu Y, Ruan J, Qian W, Wang M, Huang Q, Li B, Xuan Z, Cao J, Asan, Wu Z, Zhang J, Cai Q, Bai Y, Zhao B, Han Y, Li Y, Li X, Wang S, Shi Q, Liu S, Cho WK, Kim JY, Xu Y, Heller-Uszynska K, Miao H, Cheng Z, Zhang S, Wu J, Yang Y, Kang H, Li M, Liang H, Ren X, Shi Z, Wen M, Jian M, Yang H, Zhang G, Yang Z, Chen R, Liu S, Li J, Ma L, Liu H, Zhou Y, Zhao J, Fang X, Li G, Fang L, Li Y, Liu D, Zheng H, Zhang Y, Qin N, Li Z, Yang G, Yang S, Bolund L, Kristiansen K, Zheng H, Li S, Zhang X, Yang H, Wang J, Sun R, Zhang B, Jiang S, Wang J, Du Y, Li S
Nature genetics41(12)1275-81(2009 Dec)

An integrated genetic and cytogenetic map of the cucumber genome.
Ren Y, Zhang Z, Liu J, Staub JE, Han Y, Cheng Z, Li X, Lu J, Miao H, Kang H, Xie B, Gu X, Wang X, Du Y, Jin W, Huang S
PloS one4(6)e5795(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ERF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu74407	NM_001280596.1 Latest version!	Cucumis sativus ethylene-responsive transcription factor 4-like (ERF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ERF1 ( NM_001280596.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> NP_001267525.1 Cucumis sativus ethylene-responsive transcription factor 4-like (ERF1), mRNA.

Price & Availability↑

CloneID	OCu74407
Clone ID Related Accession (Same CDS sequence)	NM_001280596.1
Accession Version	NM_001280596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-23
Organism	Cucumis sativus(cucumber)
Product	ethylene-responsive transcription factor 4-like
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY792593.1. On Jul 23, 2013 this sequence version replaced XM_004134647.1. ##Evidence-Data-START## Transcript is intronless :: AY792593.1 [ECO:0000345] ##Evidence-Data-END##

ATGGCTCCGA GGGAGAAGGC AGTTGCCGTC AAGCCTAGCG TCGGTAACGT GAAGGAAGTG 
CATTTTAGAG GAGTGAGGAA GCGGCCATGG GGGAGATATG CCGCTGAGAT CAGAGATCCC 
AGTAAGAAAA GCCGGGTTTG GCTTGGAACC TTTGATACCG CGGAGGAGGC GGCTCGAGCC 
TACGACAGCG CCGCTAGAGA TTTCCGCGGC GTTAAGGCCA AGACCAATTT CCCTTTGCCC 
TCCGATGATC AGCTTCTTAA CCTTAACAAT AAGATTAACA ACATCAACAA TAATCAGAGC 
CCGAGTCAGA GTAGCACGGT TGAGTCCTCT AGCCGGGAGC AAGCTCTGAT GGTTGATTCC 
TCCCCTTTGA ATCTCAATCT AGGTCACGGT ATTGGCGGAC TCACGAACGC TGGACCCATC 
AGTTTCCCAT TTCAGCGTTA CCAAATTCCC ATGATCGGAG AAGTTTTTAC CCGCGGCATA 
CCGCCTTCGA ATCACGTTCT CTATTTCGAT GCGGCTCTAC GTGCTGGAAT GATCAACAGT 
CATCCGAACC AACGGCTACA CTTCGACCGT ATTCGCGAAG CGGTGAGTGA TTTCCGACGC 
GAGTTCGCCG GCAGCGGTGT TCAGAGCGAC TCCGATTCCT CCTCCGTTGT GGACATGAAC 
GGTCAAGACC TCAAGCCCCG AGGAGGAAGT GGCGGCCGCC TTGATTTGGA TCTCAACTTT 
CCCCCACCAG AATCCGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001267525.1
CDS	1..741
Translation	MAPREKAVAVKPSVGNVKEVHFRGVRKRPWGRYAAEIRDPSKKSRVWLGTFDTAEEAARAYDSAARDFRGVKAKTNFPLPSDDQLLNLNNKINNINNNQSPSQSSTVESSSREQALMVDSSPLNLNLGHGIGGLTNAGPISFPFQRYQIPMIGEVFTRGIPPSNHVLYFDAALRAGMINSHPNQRLHFDRIREAVSDFRREFAGSGVQSDSDSSSVVDMNGQDLKPRGGSGGRLDLDLNFPPPESA

Target ORF information:

RefSeq Version	NM_001280596.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus ethylene-responsive transcription factor 4-like (ERF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001280596.1

ORF Insert Sequence:

ATGGCTCCGA GGGAGAAGGC AGTTGCCGTC AAGCCTAGCG TCGGTAACGT GAAGGAAGTG 
CATTTTAGAG GAGTGAGGAA GCGGCCATGG GGGAGATATG CCGCTGAGAT CAGAGATCCC 
AGTAAGAAAA GCCGGGTTTG GCTTGGAACC TTTGATACCG CGGAGGAGGC GGCTCGAGCC 
TACGACAGCG CCGCTAGAGA TTTCCGCGGC GTTAAGGCCA AGACCAATTT CCCTTTGCCC 
TCCGATGATC AGCTTCTTAA CCTTAACAAT AAGATTAACA ACATCAACAA TAATCAGAGC 
CCGAGTCAGA GTAGCACGGT TGAGTCCTCT AGCCGGGAGC AAGCTCTGAT GGTTGATTCC 
TCCCCTTTGA ATCTCAATCT AGGTCACGGT ATTGGCGGAC TCACGAACGC TGGACCCATC 
AGTTTCCCAT TTCAGCGTTA CCAAATTCCC ATGATCGGAG AAGTTTTTAC CCGCGGCATA 
CCGCCTTCGA ATCACGTTCT CTATTTCGAT GCGGCTCTAC GTGCTGGAAT GATCAACAGT 
CATCCGAACC AACGGCTACA CTTCGACCGT ATTCGCGAAG CGGTGAGTGA TTTCCGACGC 
GAGTTCGCCG GCAGCGGTGT TCAGAGCGAC TCCGATTCCT CCTCCGTTGT GGACATGAAC 
GGTCAAGACC TCAAGCCCCG AGGAGGAAGT GGCGGCCGCC TTGATTTGGA TCTCAACTTT 
CCCCCACCAG AATCCGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA-Seq improves annotation of protein-coding genes in the cucumber genome.
BMC genomics12540(2011 Nov)
Li Z,Zhang Z,Yan P,Huang S,Fei Z,Lin K

Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types.
BMC genomics11384(2010 Jun)
Guo S,Zheng Y,Joung JG,Liu S,Zhang Z,Crasta OR,Sobral BW,Xu Y,Huang S,Fei Z

The genome of the cucumber, Cucumis sativus L.
Nature genetics41(12)1275-81(2009 Dec)
Huang S,Li R,Zhang Z,Li L,Gu X,Fan W,Lucas WJ,Wang X,Xie B,Ni P,Ren Y,Zhu H,Li J,Lin K,Jin W,Fei Z,Li G,Staub J,Kilian A,van der Vossen EA,Wu Y,Guo J,He J,Jia Z,Ren Y,Tian G,Lu Y,Ruan J,Qian W,Wang M,Huang Q,Li B,Xuan Z,Cao J,Asan,Wu Z,Zhang J,Cai Q,Bai Y,Zhao B,Han Y,Li Y,Li X,Wang S,Shi Q,Liu S,Cho WK,Kim JY,Xu Y,Heller-Uszynska K,Miao H,Cheng Z,Zhang S,Wu J,Yang Y,Kang H,Li M,Liang H,Ren X,Shi Z,Wen M,Jian M,Yang H,Zhang G,Yang Z,Chen R,Liu S,Li J,Ma L,Liu H,Zhou Y,Zhao J,Fang X,Li G,Fang L,Li Y,Liu D,Zheng H,Zhang Y,Qin N,Li Z,Yang G,Yang S,Bolund L,Kristiansen K,Zheng H,Li S,Zhang X,Yang H,Wang J,Sun R,Zhang B,Jiang S,Wang J,Du Y,Li S

An integrated genetic and cytogenetic map of the cucumber genome.
PloS one4(6)e5795(2009 Jun)
Ren Y,Zhang Z,Liu J,Staub JE,Han Y,Cheng Z,Li X,Lu J,Miao H,Kang H,Xie B,Gu X,Wang X,Du Y,Jin W,Huang S

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ERF1 cDNA ORF clone, Cucumis sativus(cucumber)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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電話

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オンラインフォーム