crim1 cDNA ORF clone,

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gRNAs

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo572639	XM_029461297.1 Latest version!	Cottoperca gobio cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_029461297.1 ) cDNA ORF clone, -> XP_029317157.1 Cottoperca gobio cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

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CloneID	OCo572639
Clone ID Related Accession (Same CDS sequence)	XM_029461297.1
Accession Version	XM_029461297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-05
Organism	Cottoperca gobio
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041376.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cottoperca gobio Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA AGTGCTCTGT TGTGTTATTG AATGTCCTTT TTCTGGTGCG GGCGCTCTCG 
GCTCTTGAGT GCACGCCGTG TAATCTCCGG ACATGCCCGG TCAAAGCCCC GCACGGCTGC 
GACGGTGGCC GGACCTTGGC CAGGGATCCG TGCGGATGCT GTGACCGGTG TACCCGGCTG 
GAGTGGGAGC CCTGCGGCGG ACAGGACTGG GCGCACGGTT ACTGCGCCTT GGGACTAACC 
TGCGCCTCCG TTAACGAAAC AAGTGCAGCC ACAATCCCTG AAACTGGAGT ATGTAAAGTG 
CTCCCTGAGC ATCCAGAGGC AGGCCTGGAA GATGAGCGCT GTCCCCTGAT GACGGGCTGC 
GACAGAGCTG GAGGTCAGTG TGTGTGCGAT GCCCGCCACT CCTGCTTGGG CTCTTTCACT 
TACCCAGACC AAGAAACCTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG GCACGAGCAC 
CGGGAAAGAC ACAGAGAGAA GTATGTGGGA CCATCCAACC CGGCCTGCAT GTTCTCTGGG 
TGTAACCTGA CCGCGGAGGG CTGTGTGTGC GAGTCTCAGA GCTGCCACCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCC GCAGAGGAGC TGAGGTGTGC CGGGGTGATG 
TGTCCCGCTC TGCAGGTGCC CTCCTGCCCC AAGGGTTCAG TCCTGACCAA GAGTCACACG 
GCCCCTGCTG GATGCTGCCC CTCCGTACCG CCCCAGTGCA CCTGCGACCT CCGTGCCTGC 
CACAAACCCC ATTGTCCGAG CGGACAGCGT GCCGTGCCAC TCAGCGAGGC CAGCGGTCAG 
CCGGGAGACT GCTGTGATGT CTTTGAGTGC CAGAAAGTCT CTCCCAAGTG TGTCCACAAT 
GGGAAGGAGT TTTCTGAAGG AGAGGTGTAT CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGGGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGCGCTG AGCTGGACTG TGAAAACTTC 
TACATTCCTG AGGGCGAGTG TTGCCCTGTA TGCATAGATG TAGAGTTACT CTCGATGGAC 
AGCACTAAGG CCAGTTGCTG GGTGAATAAT AAGCTGCGGG CCCATGAGGA ACAGTGGAAA 
GAAGACGACT GCACGTTCTG CCAGTGTGTG GATGGAGAAC CGCACTGCAC GGCCATGGCC 
TGCAAACAGA GTTGCCAGTA CCCTGTCAAG ATCCCTGGAG AGTGCTGTCC CTTCTGTGAA 
GAGCCTTCTT ATGAAACGGT TAGCCCCATG CTATGCCCTC CCCTGGAAAA CTGCTCCCTG 
TCAGGGAACA ACTGCCCCTA CGGCTTCCAA CAAGACAATA ACGGATGTCT GCTCTGCCAG 
TGTCTCAACA ATGACTCCTG CCCTGACTTA GGAAAATACT GTTCCCTGCA CTGCCCAATG 
GGGTACAAGA GGGATGACTT TGGCTGTGAT GTGTGTGAGT GCAGCATCCC CGTGCCCAAG 
TGCCGACCTT TGACCTGCAC TAAGACCTGC CCCTATGGAT ATGTGAGGAA CAAGCATGGC 
TGTGAGATGT GTCGCTGCGT CAAGTGTCCT CCCTTCACCT GTGACAAGCA CTGCAGCGAT 
GGCTATCGAC AGAACAGGAA GGGCTGCTCC ATCTGCATGT GTAAAGAAAG TGGCCACGTC 
CCTAGCACCA CTGCCCCGGC CCCGATGCCC ACCTACTGCC TCACGTCGAA TGGCCACCGA 
TACGAGGAAG GTGACGGCTG GCACGACGGT TGTCGTGACT GCTACTGCCA CTCCGGACGG 
GAGATGTGTG TGCTTATATC CTGCCCCGTG CCCATCTGTT CTCATCCAGT GGTGAGGCCT 
GACCAGTGTT GTCCTACATG TGAAGATGAG TCAGGCAGCG GTCAGCCTGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGAGGGGAA TTTTACGTGG AGGGGGAGAC CTGGAACCTG 
GACGAGTGCA CGCGGTGCAC CTGCAGGAAG GGACGTGTCC TGTGCGACAC TGAAGTGTGT 
CCCCCGGCTC TCTGCCAGAC ACCAATCAGG AACAAGGACA CCTGTTGCCA CGTATGCGCA 
GAGGAGCTGA GCCCATTGCT GCCGGTGAAC AACAGCCAGC AGGAGTACTG CATCACCAGT 
GATGGAGACG TGCTGCTGGC TGGGGACTCC TGGAAGGCCA ACGCCTGCAC CAGCTGCACC 
TGCAACAACG GCACCATTCA GTGTTTCTCT CAGCACTGTC CAGCTGCCAA CTGCAGGGTG 
CCCGTCTTAC GTAAGGGCCA GTGCTGCCCA CACTGTCTCG AAATTACGAC TTCAGTGCCG 
GTGATGGTGT CCACCAACGC TCCCAAGACA ACGGCCCCCA TTACAGTGGA GAGTGCAGTG 
TGGATCACCA CTGTCACTGT ACCGCCCATC ATTGCTGAGA CAGATGAGCC AGTTTTGGGT 
GAGAATTACC CCAGCCAAAC AGAGATGGCT GTCATCTACC AATCAGCAGC CTGGATTCTG 
GCAGGTCTCC TCCTCGCCAT CATCATCTTT ATCGTCGTGG CGTTACTCAT CAACAAGAAA 
AAGAAGTGGG TGCAGATGTC CTGCTACAGT GCACCAAAGA AGACGGTGAT CCTAAAGAAG 
CACGTGAACA AGAACTCAGT GGTCTACATG GAGCCCTCCA GAGAGAACAA ATTTCAGAAT 
GTGAAGAACG ACTGCGGCAT TATCCATTTC TCTCCAGAAA TGAGCTCCAC CTGTGTGGAC 
AAGATGATCA TCCCCAGGGC CAAGCTGAGC AACGACTGGA CGCAGCGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029317157.1
CDS	345..3155
Translation	MFSKCSVVLLNVLFLVRALSALECTPCNLRTCPVKAPHGCDGGRTLARDPCGCCDRCTRLEWEPCGGQDWAHGYCALGLTCASVNETSAATIPETGVCKVLPEHPEAGLEDERCPLMTGCDRAGGQCVCDARHSCLGSFTYPDQETCMKASKSEGRRHEHRERHREKYVGPSNPACMFSGCNLTAEGCVCESQSCHHHFAYINRSQCQEAAEELRCAGVMCPALQVPSCPKGSVLTKSHTAPAGCCPSVPPQCTCDLRACHKPHCPSGQRAVPLSEASGQPGDCCDVFECQKVSPKCVHNGKEFSEGEVYRMDPCWLCQCRGGISFCSKAECAELDCENFYIPEGECCPVCIDVELLSMDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGEPHCTAMACKQSCQYPVKIPGECCPFCEEPSYETVSPMLCPPLENCSLSGNNCPYGFQQDNNGCLLCQCLNNDSCPDLGKYCSLHCPMGYKRDDFGCDVCECSIPVPKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSDGYRQNRKGCSICMCKESGHVPSTTAPAPMPTYCLTSNGHRYEEGDGWHDGCRDCYCHSGREMCVLISCPVPICSHPVVRPDQCCPTCEDESGSGQPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPIRNKDTCCHVCAEELSPLLPVNNSQQEYCITSDGDVLLAGDSWKANACTSCTCNNGTIQCFSQHCPAANCRVPVLRKGQCCPHCLEITTSVPVMVSTNAPKTTAPITVESAVWITTVTVPPIIAETDEPVLGENYPSQTEMAVIYQSAAWILAGLLLAIIIFIVVALLINKKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSRENKFQNVKNDCGIIHFSPEMSSTCVDKMIIPRAKLSNDWTQR

Target ORF information:

RefSeq Version	XM_029461297.1
Organism	Cottoperca gobio
Definition	Cottoperca gobio cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029461297.1

ORF Insert Sequence:

ATGTTCAGCA AGTGCTCTGT TGTGTTATTG AATGTCCTTT TTCTGGTGCG GGCGCTCTCG 
GCTCTTGAGT GCACGCCGTG TAATCTCCGG ACATGCCCGG TCAAAGCCCC GCACGGCTGC 
GACGGTGGCC GGACCTTGGC CAGGGATCCG TGCGGATGCT GTGACCGGTG TACCCGGCTG 
GAGTGGGAGC CCTGCGGCGG ACAGGACTGG GCGCACGGTT ACTGCGCCTT GGGACTAACC 
TGCGCCTCCG TTAACGAAAC AAGTGCAGCC ACAATCCCTG AAACTGGAGT ATGTAAAGTG 
CTCCCTGAGC ATCCAGAGGC AGGCCTGGAA GATGAGCGCT GTCCCCTGAT GACGGGCTGC 
GACAGAGCTG GAGGTCAGTG TGTGTGCGAT GCCCGCCACT CCTGCTTGGG CTCTTTCACT 
TACCCAGACC AAGAAACCTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG GCACGAGCAC 
CGGGAAAGAC ACAGAGAGAA GTATGTGGGA CCATCCAACC CGGCCTGCAT GTTCTCTGGG 
TGTAACCTGA CCGCGGAGGG CTGTGTGTGC GAGTCTCAGA GCTGCCACCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCC GCAGAGGAGC TGAGGTGTGC CGGGGTGATG 
TGTCCCGCTC TGCAGGTGCC CTCCTGCCCC AAGGGTTCAG TCCTGACCAA GAGTCACACG 
GCCCCTGCTG GATGCTGCCC CTCCGTACCG CCCCAGTGCA CCTGCGACCT CCGTGCCTGC 
CACAAACCCC ATTGTCCGAG CGGACAGCGT GCCGTGCCAC TCAGCGAGGC CAGCGGTCAG 
CCGGGAGACT GCTGTGATGT CTTTGAGTGC CAGAAAGTCT CTCCCAAGTG TGTCCACAAT 
GGGAAGGAGT TTTCTGAAGG AGAGGTGTAT CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGGGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGCGCTG AGCTGGACTG TGAAAACTTC 
TACATTCCTG AGGGCGAGTG TTGCCCTGTA TGCATAGATG TAGAGTTACT CTCGATGGAC 
AGCACTAAGG CCAGTTGCTG GGTGAATAAT AAGCTGCGGG CCCATGAGGA ACAGTGGAAA 
GAAGACGACT GCACGTTCTG CCAGTGTGTG GATGGAGAAC CGCACTGCAC GGCCATGGCC 
TGCAAACAGA GTTGCCAGTA CCCTGTCAAG ATCCCTGGAG AGTGCTGTCC CTTCTGTGAA 
GAGCCTTCTT ATGAAACGGT TAGCCCCATG CTATGCCCTC CCCTGGAAAA CTGCTCCCTG 
TCAGGGAACA ACTGCCCCTA CGGCTTCCAA CAAGACAATA ACGGATGTCT GCTCTGCCAG 
TGTCTCAACA ATGACTCCTG CCCTGACTTA GGAAAATACT GTTCCCTGCA CTGCCCAATG 
GGGTACAAGA GGGATGACTT TGGCTGTGAT GTGTGTGAGT GCAGCATCCC CGTGCCCAAG 
TGCCGACCTT TGACCTGCAC TAAGACCTGC CCCTATGGAT ATGTGAGGAA CAAGCATGGC 
TGTGAGATGT GTCGCTGCGT CAAGTGTCCT CCCTTCACCT GTGACAAGCA CTGCAGCGAT 
GGCTATCGAC AGAACAGGAA GGGCTGCTCC ATCTGCATGT GTAAAGAAAG TGGCCACGTC 
CCTAGCACCA CTGCCCCGGC CCCGATGCCC ACCTACTGCC TCACGTCGAA TGGCCACCGA 
TACGAGGAAG GTGACGGCTG GCACGACGGT TGTCGTGACT GCTACTGCCA CTCCGGACGG 
GAGATGTGTG TGCTTATATC CTGCCCCGTG CCCATCTGTT CTCATCCAGT GGTGAGGCCT 
GACCAGTGTT GTCCTACATG TGAAGATGAG TCAGGCAGCG GTCAGCCTGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGAGGGGAA TTTTACGTGG AGGGGGAGAC CTGGAACCTG 
GACGAGTGCA CGCGGTGCAC CTGCAGGAAG GGACGTGTCC TGTGCGACAC TGAAGTGTGT 
CCCCCGGCTC TCTGCCAGAC ACCAATCAGG AACAAGGACA CCTGTTGCCA CGTATGCGCA 
GAGGAGCTGA GCCCATTGCT GCCGGTGAAC AACAGCCAGC AGGAGTACTG CATCACCAGT 
GATGGAGACG TGCTGCTGGC TGGGGACTCC TGGAAGGCCA ACGCCTGCAC CAGCTGCACC 
TGCAACAACG GCACCATTCA GTGTTTCTCT CAGCACTGTC CAGCTGCCAA CTGCAGGGTG 
CCCGTCTTAC GTAAGGGCCA GTGCTGCCCA CACTGTCTCG AAATTACGAC TTCAGTGCCG 
GTGATGGTGT CCACCAACGC TCCCAAGACA ACGGCCCCCA TTACAGTGGA GAGTGCAGTG 
TGGATCACCA CTGTCACTGT ACCGCCCATC ATTGCTGAGA CAGATGAGCC AGTTTTGGGT 
GAGAATTACC CCAGCCAAAC AGAGATGGCT GTCATCTACC AATCAGCAGC CTGGATTCTG 
GCAGGTCTCC TCCTCGCCAT CATCATCTTT ATCGTCGTGG CGTTACTCAT CAACAAGAAA 
AAGAAGTGGG TGCAGATGTC CTGCTACAGT GCACCAAAGA AGACGGTGAT CCTAAAGAAG 
CACGTGAACA AGAACTCAGT GGTCTACATG GAGCCCTCCA GAGAGAACAA ATTTCAGAAT 
GTGAAGAACG ACTGCGGCAT TATCCATTTC TCTCCAGAAA TGAGCTCCAC CTGTGTGGAC 
AAGATGATCA TCCCCAGGGC CAAGCTGAGC AACGACTGGA CGCAGCGCTA A