EDEM1 cDNA ORF clone, Colobus angolensis palliatus

Gene
Clones
gRNAs

Summary

Gene Symbol	EDEM1
Entrez Gene ID	105508388
Full Name	ER degradation enhancing alpha-mannosidase like protein 1
Gene Type	protein-coding
Organism	Colobus angolensis palliatus

mRNA and Protein(s)

mRNA	Protein	Name
XM_011937258.1	XP_011792648.1	ER degradation-enhancing alpha-mannosidase-like protein 1

Pathways from BioSystems
Gene Ontology

The following EDEM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDEM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo184577	XM_011937258.1 Latest version!	Colobus angolensis palliatus ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDEM1 ( XM_011937258.1 ) cDNA ORF clone, Colobus angolensis palliatus -> XP_011792648.1 Colobus angolensis palliatus ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), mRNA.

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CloneID	OCo184577
Clone ID Related Accession (Same CDS sequence)	XM_011937258.1
Accession Version	XM_011937258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-29
Organism	Colobus angolensis palliatus
Product	ER degradation-enhancing alpha-mannosidase-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012114711.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Colobus angolensis palliatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATGGC GAGCGCTTGT CCTGGGGCTG GTGCTCCTCC GGCTTGGCCT CCATGGAGTA 
TTGTGGCTCG TCTTCGGGCT GGGGCCCAGC ATGGGCTTCT ACCAGCGCTT TCCGCTCAGC 
TTCGGCTTCC AGCGTCTGAG GAGCCCCGAC GGCCCCGCGT CGCCCGCCTC GGGGCCCGTG 
GGCCGGCCTG GGGGGCTATC CGGACCGTCG TGGCTGCAGC CGCCAGGGAC CGGGGCGACG 
CAGAGCCCGC GCAAGGTTCC GCAGCGTTCT GAGCCGGGGG TGTGCGGCCC AGCCCACTGG 
GGCTACGTGC TGGGCGGCCG GGACCGCGGC CCGGACGAGT ACGAGAAGCG CTACAGCGGC 
GCCTTCCCTC CGCAGCTGCG TGCCCAGATG CGCGACCTGG CACGGGGGAT GTTCGTCTTT 
GGCTACGACA ACTACATGGC GCACGCCTTC CCTCAGGACG AGCTCAACCC CATCCACTGC 
CGCGGCCGTG GGCCCGACCG CGGGGACCCT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAGTAATGGG AAATTCATCC 
GAGTTCCAGA AAGCAGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC AATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACCGATT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATATATGG CCCATGACCT GGCAGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATCCCGT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTCG GGATTCTGAG TCGACTGCTG 
GGGGACTCCA CATTTGAGTG GGTAGCCAGG CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CGGGATTACT AGGCAATGTT GTGAACATTC AGACTGGCCA TTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GTGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC CCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTACAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ATGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA CTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCACGAGTTA 
AAAGTCATCA ACTCCAGCTC CAATTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGCTTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011792648.1
CDS	235..2208
Translation	MQWRALVLGLVLLRLGLHGVLWLVFGLGPSMGFYQRFPLSFGFQRLRSPDGPASPASGPVGRPGGLSGPSWLQPPGTGATQSPRKVPQRSEPGVCGPAHWGYVLGGRDRGPDEYEKRYSGAFPPQLRAQMRDLARGMFVFGYDNYMAHAFPQDELNPIHCRGRGPDRGDPSNLNINDVLGNYSLTLVDALDTLAVMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

Target ORF information:

RefSeq Version	XM_011937258.1
Organism	Colobus angolensis palliatus
Definition	Colobus angolensis palliatus ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011937258.1

ORF Insert Sequence:

ATGCAATGGC GAGCGCTTGT CCTGGGGCTG GTGCTCCTCC GGCTTGGCCT CCATGGAGTA 
TTGTGGCTCG TCTTCGGGCT GGGGCCCAGC ATGGGCTTCT ACCAGCGCTT TCCGCTCAGC 
TTCGGCTTCC AGCGTCTGAG GAGCCCCGAC GGCCCCGCGT CGCCCGCCTC GGGGCCCGTG 
GGCCGGCCTG GGGGGCTATC CGGACCGTCG TGGCTGCAGC CGCCAGGGAC CGGGGCGACG 
CAGAGCCCGC GCAAGGTTCC GCAGCGTTCT GAGCCGGGGG TGTGCGGCCC AGCCCACTGG 
GGCTACGTGC TGGGCGGCCG GGACCGCGGC CCGGACGAGT ACGAGAAGCG CTACAGCGGC 
GCCTTCCCTC CGCAGCTGCG TGCCCAGATG CGCGACCTGG CACGGGGGAT GTTCGTCTTT 
GGCTACGACA ACTACATGGC GCACGCCTTC CCTCAGGACG AGCTCAACCC CATCCACTGC 
CGCGGCCGTG GGCCCGACCG CGGGGACCCT TCAAATCTGA ACATCAATGA TGTACTAGGG 
AACTACTCAT TGACTCTTGT TGATGCATTG GATACACTTG CAGTAATGGG AAATTCATCC 
GAGTTCCAGA AAGCAGTCAA GTTAGTGATC AACACAGTTT CATTTGACAA AGATTCCACC 
GTCCAAGTCT TTGAGGCCAC AATAAGGGTC CTGGGAAGCC TCCTTTCTGC TCACAGAATA 
ATAACCGATT CCAAGCAGCC CTTTGGTGAC ATGACAATTA AGGACTATGA TAATGAGTTG 
TTATATATGG CCCATGACCT GGCAGTGCGG CTCCTCCCTG CTTTTGAAAA CACCAAGACA 
GGGATCCCGT ATCCTCGGGT GAATCTAAAG ACAGGAGTTC CTCCTGACAC CAATAATGAG 
ACATGCACAG CGGGAGCCGG TTCCCTCCTG GTGGAATTCG GGATTCTGAG TCGACTGCTG 
GGGGACTCCA CATTTGAGTG GGTAGCCAGG CGAGCAGTGA AAGCCCTTTG GAACCTCCGG 
AGCAATGATA CGGGATTACT AGGCAATGTT GTGAACATTC AGACTGGCCA TTGGGTTGGA 
AAGCAGAGTG GCCTGGGTGC CGGGCTGGAC TCCTTCTATG AATACCTCTT GAAATCTTAC 
ATTCTCTTTG GTGAAAAAGA AGACCTAGAA ATGTTTAATG CTGCATATCA GAGTATTCAG 
AACTACTTAA GAAGAGGGCG GGAAGCCTGC AATGAAGGAG AAGGAGACCC CCCACTCTAT 
GTCAACGTGA ACATGTTCAG TGGGCAGCTG ATGAACACCT GGATTGACTC TCTACAGGCC 
TTTTTCCCTG GACTGCAGGT GCTGATAGGA GATGTGGAAG ATGCCATCTG CCTTCATGCC 
TTCTACTATG CCATATGGAA ACGATATGGT GCCCTCCCTG AGAGATATAA CTGGCAGCTG 
CAGGCCCCTG ATGTTCTCTT CTACCCACTG AGACCAGAGT TAGTGGAATC CACATATCTC 
CTCTACCAGG CAACCAAGAA TCCCTTCTAC CTCCATGTAG GAATGGATAT TCTGCAGAGT 
CTGGAAAAGT ACACAAAAGT CAAGTGTGGG TACGCCACGC TGCATCACGT CATTGACAAG 
TCCACAGAAG ACCGGATGGA GAGCTTCTTT CTCAGTGAGA CCTGTAAATA CTTGTATCTG 
CTGTTTGATG AAGACAATCC AGTACACAAG TCTGGAACCA GATACATGTT CACAACAGAG 
GGACACATTG TATCTGTGGA TGAGCATCTT CGGGAATTGC CATGGAAGGA ATTCTTCTCT 
GAAGAGGGAG GGCAGGACCA AGGGGGAAAG TCTGTGCACA GGCCGAAACC TCACGAGTTA 
AAAGTCATCA ACTCCAGCTC CAATTGCAAT CGTGTACCTG ATGAGAGGAG GTACTCCCTG 
CCCTTAAAGA GCATCTACAT GCGACAGATT GACCAGATGG TTGGCTTGAT TTGA