LOC778532 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC778532
Entrez Gene ID	778532
Full Name	adenylate cyclase type 5
Synonyms	rut
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_026837626.1	XP_026693427.1	adenylate cyclase type 6
XM_018814717.1	XP_018670262.1	adenylate cyclase type 5

Pathways from BioSystems
Gene Ontology

The following LOC778532 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC778532 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCi154676	XM_026837626.1 Latest version!	Ciona intestinalis adenylate cyclase type 6 (LOC778532), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCi71861	XM_018814717.1 Latest version!	Ciona intestinalis adenylate cyclase type 5 (LOC778532), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC778532 ( XM_026837626.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_026693427.1 Ciona intestinalis adenylate cyclase type 6 (LOC778532), mRNA.

Price & Availability↑

CloneID	OCi154676
Clone ID Related Accession (Same CDS sequence)	XM_026837626.1
Accession Version	XM_026837626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	adenylate cyclase type 6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020179.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGATTCTAC TCAGCCACGT CGTTTTGCAT AACGATCTAC TAGCCACGAA CATTTACGAA 
GATACAAATA TGCAGAAGGA AGAGAGAAAA GGTCTGCTTC GGAATCACGC TTCACTTAAG 
GAAATGACTA CATGTGAGAG CCCTAACTCA TCTAAGATTG AACAGCTAAA GCATTTAAAA 
GATGCCAACG GGGCAACCCC CTCCCCCCCT TTATCCGAGA GGATATTAAA GCGACTGAAC 
CCGAAAATGA GGAAAGCGGG GTCTTTAGAC GAGAACTTGT CCAATTCCCG CCCTGCTACC 
CCTACAACGG GAAGAAACCA TAAAAAGACC CAAGAACGCA TGGGTTCCGC CCCTGTTCCT 
GCCAATTCCC CTCAAGCCTC CACCAAGAAG AGAAACTTCT CTATCAGGCA TAGGACTTTA 
GACTCTGAGG GTGACACCCC TATGTTACCC ATGCAGTCGT TTTCACCTAT ACAGCAACCT 
GAACCCACCC CGGAAGTGGA ATTAAATTCA CGCTCCCCGA AAATGTTAAC GAAATGCTGC 
TTCTGGTCTT CATGCGCTGA AATGCTGCAC AAATTCATGT CTGGAGTCCG TTTTGAATCC 
TGGAGCTCTG AACGTCTCTA CCAGCGCTAC TTCTTCCATT CCAAGATAAA TATGCTTAGC 
ATACTGCTAT GGTTACTCAA ATCTCTTTGC ATCATTTTAT TTTGTTTTAA ATATTGTACA 
GGACAAAATT GGAACGACAT GGTGTATGGA ATTATTATCA TTGTATTGCT TCTACTGTGT 
ATTGCCTTGC AGGTGATCTT TCTTCTCAAT GTACTTTCCC AGCACACATT ATTCTTGGTG 
TCTTACATCA TACTAGCCAT GGTTGTTATC ATTGATCTAG TGTCAGTTGT ACTTTTAGAG 
CGGTCAAGCT CAAGCTACAT GGTATGGCTC ACTTTGTTCA TGGTATACAT TATGTACACC 
ATGATACCTC TGAACATTCT GGTGGTGTCT GCGGCCAGTT TTATCCTCTC CTGCAGCCAT 
ATGCTTGTCT CCTATGTTTC AAATGGACCC AAGCCCCATT ACCCCAACCA GATTGCTTCC 
GACTTCCTTT TGTTTTTCTG CACCAACGTG GTCGGCATGT GCATTCGTAT AACATCAGAA 
CTTGCTCAGC GAAAAGCTTT TACTGAGACG CGAGAATGTA TCGTCGCTCG TCTTAATCTG 
CAAAGGCAGA ATCTGCACCA GGAAAGGTTG TTACTTTCTG TTCTCCCACG TCACGTTGCT 
ATGGAAATGA AAGGTGACAT TGACCAATCG AAACCCAAGA CACAACAATT CTATAAAATT 
TACATCCAAA AGCACAACAA CGTTAGCATA TTGTACGCTG ATATTGAAGG GTTTACGAAA 
CTTGCTTCAC AATGTCAACC CCAGTGGCTT GTTCGGACAT TAAATCAACT TTTCGCACGG 
TTTGATGCTC TAGCACGGGA CAACAACTGC CTACGAATCA AGATTCTTGG AGATTGCTAT 
TATTGCGTGG CTGGCTTACC CGAGCCTAGA CCTGACCACG CGCATGCTTG TGTACAAATG 
GGCTTGGACA TGATAGACAC AATATCTTTA ATTCGGGAAA TGACCGGCAT ACCAGAATTA 
AACATGAGAG TTGGTATTCA CACTGGTCGT GTACACTGTG GGGTACTTGG TCTCAAAAAA 
TGGCAGTTTG ACGTTTGGAG TAACGATGTC ACACTAGCCA ACCTTATGGA AGCTGGCGGC 
AAAGCAGGGC GCATTCATAT AACTGCAGCT ACACTTAAGT ATTTACACGA CGATTACGAA 
GTGGAACCCG GCTATGGTTA CGAACGCAAC AGTAAACTAC AAGGTGTGGA AACTTACCTC 
ATAGTCAAGC AAAAGCCTCA AGCAAAGTTG CCGACCATAA TGCCGCAGGT CAGTACCAAC 
GAGTCCTCAC TGCCGGGACA ACCAGTTCCA ACACATACCA ACAACACAAG GAGAAATAAG 
AACAAAACAG CCAAGGAAAT GAAATTAATG GGCTTTGAAT ACTCAAAGAA CCTTTCCCAC 
GATTTACTGA AAGACAACAA CAGTATTTTA TCCGACGGCG AAGTAAACGA CTTTTTGAAC 
CGCGCAATTG ACGCACAAGG ACTTGACTTT ATCAAGTCAA ACCAGCTCAG CAAATACTTT 
CGGTCTTTCA AGGATAGAAA GCTAAAGGAA GAGGTGACAT TTTGGATATA TTTTCTATCA 
GATAAAACCT ATATTAGAGT GGGAGATGGG ACACCTTTTT ATCTTCCACT ACGCCAATGT 
CAGCAACGCA TGTTTGCCGT CTACTGTGCG ACAGCAGGTC TTATTCTTCC TTTCTTTCTG 
GTTTCCACGA TCTGTTACAA GATGCTGAAA AAGTCCGACT GTAGCTGCAT AAAACGTAAA 
GCTGTCACCT TTGTCTCAGT ATCAATTATT GCCCTAATTT CAAGCATGGC AGTGGGGAAT 
ATGTTCTTCT TTCTGCCTAC GCATGATTTA CGCACATGCG CGGCACAATT CGCTAACGTC 
ACTCCAACTG CGTTGACCCC ATCCAACGTA ACAGACATAC TCAATATAAC ATTGTTGGAC 
GAAAGCAAAC CAACGATATG TGGACCACAA TCTTGCCATT TTCCACAGTA CTTCACTTAC 
AGCATGGTGC TGTCTATGCT TGCCTGTGCC CAATTTCTTG GTACGTCGGG ATTCTCGAAA 
TTAATTTTGC TTCTCAGCCA GTCCGTGTTG TACACGTTGC TGGTACTTCT GGTAAACCCT 
ACGTTGTTTG ACAATGCTGA TCTGCTGAAC CATGCTACAT TCGAAGAAAG CGTGATGATA 
CCTCTACGTT TGATGTCTCC GTTCATTTTG ATGCTGTTCG TTGCTGCGGT GCTCATGCAA 
GGCCAACAGG CAGAGCTCAC AGCGAGGATG GATTTTCTAT GGAACAAACA GGCTGCTGAA 
GAAAGAGAAG AAATGGTGAA GAAACAAGCT TATAACCGGA AACTTATTCA CAATATCTTG 
CCTGTAGATG TAGCCAAGTA TTATCTTGAT AAGAAAGAAA AAAATGACGA TGATCTTTAC 
TGCCAGTCGT GTGAATGTGT CGCCGTTATG TTCGCAACAA TAACCAATTT CTCTGAGTTT 
TATATGGAAT TAGACGCAAA TAATGAAGGC GTCGAATGCC TGAGATTGCT CAACGAAATT 
ATCGCCGATT TTGACAATAT CATTAGTGAA CCGCAGTTTT ACCAGCTTGA AAAAATCAAA 
ACGATGGGCA GTACATATAT GGTCTCGTCG GGGCTAAATA GCGCAACTTA TGATAAAGAA 
AATATGAGCC ACATTTCAGC ACTCGCGGTT TTTGCCATGC GACTACAAGA CCAGCTGAAA 
TTTGTCAATA CACATTCGTT TAATAACTTC AAATTCAGAA TAGGGTTAAA CTGCGGACCA 
GTGGTTGCAG GGGTAATAGG GGCTCGTAAA CCCCAATATG ATATATGGGG CAATACGGTA 
AACGTTGCAA GTAGAATGGA CAGTACTGGT ATAGACGACA AAATTCAGGT TTCAGAGGAC 
GTTTACAATC TCCTAAAAAC AAAGGGTTTT GATTTTGTTC CTCGCGGGTT TGTGCCTGTT 
AAAGGGAAAG GTACCATGAT GACGTATTTC CTCGAAGGCC CACGGCCCAA CGAGGAACCC 
ACGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026693427.1
CDS	102..3770
Translation	MILLSHVVLHNDLLATNIYEDTNMQKEERKGLLRNHASLKEMTTCESPNSSKIEQLKHLKDANGATPSPPLSERILKRLNPKMRKAGSLDENLSNSRPATPTTGRNHKKTQERMGSAPVPANSPQASTKKRNFSIRHRTLDSEGDTPMLPMQSFSPIQQPEPTPEVELNSRSPKMLTKCCFWSSCAEMLHKFMSGVRFESWSSERLYQRYFFHSKINMLSILLWLLKSLCIILFCFKYCTGQNWNDMVYGIIIIVLLLLCIALQVIFLLNVLSQHTLFLVSYIILAMVVIIDLVSVVLLERSSSSYMVWLTLFMVYIMYTMIPLNILVVSAASFILSCSHMLVSYVSNGPKPHYPNQIASDFLLFFCTNVVGMCIRITSELAQRKAFTETRECIVARLNLQRQNLHQERLLLSVLPRHVAMEMKGDIDQSKPKTQQFYKIYIQKHNNVSILYADIEGFTKLASQCQPQWLVRTLNQLFARFDALARDNNCLRIKILGDCYYCVAGLPEPRPDHAHACVQMGLDMIDTISLIREMTGIPELNMRVGIHTGRVHCGVLGLKKWQFDVWSNDVTLANLMEAGGKAGRIHITAATLKYLHDDYEVEPGYGYERNSKLQGVETYLIVKQKPQAKLPTIMPQVSTNESSLPGQPVPTHTNNTRRNKNKTAKEMKLMGFEYSKNLSHDLLKDNNSILSDGEVNDFLNRAIDAQGLDFIKSNQLSKYFRSFKDRKLKEEVTFWIYFLSDKTYIRVGDGTPFYLPLRQCQQRMFAVYCATAGLILPFFLVSTICYKMLKKSDCSCIKRKAVTFVSVSIIALISSMAVGNMFFFLPTHDLRTCAAQFANVTPTALTPSNVTDILNITLLDESKPTICGPQSCHFPQYFTYSMVLSMLACAQFLGTSGFSKLILLLSQSVLYTLLVLLVNPTLFDNADLLNHATFEESVMIPLRLMSPFILMLFVAAVLMQGQQAELTARMDFLWNKQAAEEREEMVKKQAYNRKLIHNILPVDVAKYYLDKKEKNDDDLYCQSCECVAVMFATITNFSEFYMELDANNEGVECLRLLNEIIADFDNIISEPQFYQLEKIKTMGSTYMVSSGLNSATYDKENMSHISALAVFAMRLQDQLKFVNTHSFNNFKFRIGLNCGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGIDDKIQVSEDVYNLLKTKGFDFVPRGFVPVKGKGTMMTYFLEGPRPNEEPTS

Target ORF information:

RefSeq Version	XM_026837626.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis adenylate cyclase type 6 (LOC778532), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026837626.1

ORF Insert Sequence:

ATGATTCTAC TCAGCCACGT CGTTTTGCAT AACGATCTAC TAGCCACGAA CATTTACGAA 
GATACAAATA TGCAGAAGGA AGAGAGAAAA GGTCTGCTTC GGAATCACGC TTCACTTAAG 
GAAATGACTA CATGTGAGAG CCCTAACTCA TCTAAGATTG AACAGCTAAA GCATTTAAAA 
GATGCCAACG GGGCAACCCC CTCCCCCCCT TTATCCGAGA GGATATTAAA GCGACTGAAC 
CCGAAAATGA GGAAAGCGGG GTCTTTAGAC GAGAACTTGT CCAATTCCCG CCCTGCTACC 
CCTACAACGG GAAGAAACCA TAAAAAGACC CAAGAACGCA TGGGTTCCGC CCCTGTTCCT 
GCCAATTCCC CTCAAGCCTC CACCAAGAAG AGAAACTTCT CTATCAGGCA TAGGACTTTA 
GACTCTGAGG GTGACACCCC TATGTTACCC ATGCAGTCGT TTTCACCTAT ACAGCAACCT 
GAACCCACCC CGGAAGTGGA ATTAAATTCA CGCTCCCCGA AAATGTTAAC GAAATGCTGC 
TTCTGGTCTT CATGCGCTGA AATGCTGCAC AAATTCATGT CTGGAGTCCG TTTTGAATCC 
TGGAGCTCTG AACGTCTCTA CCAGCGCTAC TTCTTCCATT CCAAGATAAA TATGCTTAGC 
ATACTGCTAT GGTTACTCAA ATCTCTTTGC ATCATTTTAT TTTGTTTTAA ATATTGTACA 
GGACAAAATT GGAACGACAT GGTGTATGGA ATTATTATCA TTGTATTGCT TCTACTGTGT 
ATTGCCTTGC AGGTGATCTT TCTTCTCAAT GTACTTTCCC AGCACACATT ATTCTTGGTG 
TCTTACATCA TACTAGCCAT GGTTGTTATC ATTGATCTAG TGTCAGTTGT ACTTTTAGAG 
CGGTCAAGCT CAAGCTACAT GGTATGGCTC ACTTTGTTCA TGGTATACAT TATGTACACC 
ATGATACCTC TGAACATTCT GGTGGTGTCT GCGGCCAGTT TTATCCTCTC CTGCAGCCAT 
ATGCTTGTCT CCTATGTTTC AAATGGACCC AAGCCCCATT ACCCCAACCA GATTGCTTCC 
GACTTCCTTT TGTTTTTCTG CACCAACGTG GTCGGCATGT GCATTCGTAT AACATCAGAA 
CTTGCTCAGC GAAAAGCTTT TACTGAGACG CGAGAATGTA TCGTCGCTCG TCTTAATCTG 
CAAAGGCAGA ATCTGCACCA GGAAAGGTTG TTACTTTCTG TTCTCCCACG TCACGTTGCT 
ATGGAAATGA AAGGTGACAT TGACCAATCG AAACCCAAGA CACAACAATT CTATAAAATT 
TACATCCAAA AGCACAACAA CGTTAGCATA TTGTACGCTG ATATTGAAGG GTTTACGAAA 
CTTGCTTCAC AATGTCAACC CCAGTGGCTT GTTCGGACAT TAAATCAACT TTTCGCACGG 
TTTGATGCTC TAGCACGGGA CAACAACTGC CTACGAATCA AGATTCTTGG AGATTGCTAT 
TATTGCGTGG CTGGCTTACC CGAGCCTAGA CCTGACCACG CGCATGCTTG TGTACAAATG 
GGCTTGGACA TGATAGACAC AATATCTTTA ATTCGGGAAA TGACCGGCAT ACCAGAATTA 
AACATGAGAG TTGGTATTCA CACTGGTCGT GTACACTGTG GGGTACTTGG TCTCAAAAAA 
TGGCAGTTTG ACGTTTGGAG TAACGATGTC ACACTAGCCA ACCTTATGGA AGCTGGCGGC 
AAAGCAGGGC GCATTCATAT AACTGCAGCT ACACTTAAGT ATTTACACGA CGATTACGAA 
GTGGAACCCG GCTATGGTTA CGAACGCAAC AGTAAACTAC AAGGTGTGGA AACTTACCTC 
ATAGTCAAGC AAAAGCCTCA AGCAAAGTTG CCGACCATAA TGCCGCAGGT CAGTACCAAC 
GAGTCCTCAC TGCCGGGACA ACCAGTTCCA ACACATACCA ACAACACAAG GAGAAATAAG 
AACAAAACAG CCAAGGAAAT GAAATTAATG GGCTTTGAAT ACTCAAAGAA CCTTTCCCAC 
GATTTACTGA AAGACAACAA CAGTATTTTA TCCGACGGCG AAGTAAACGA CTTTTTGAAC 
CGCGCAATTG ACGCACAAGG ACTTGACTTT ATCAAGTCAA ACCAGCTCAG CAAATACTTT 
CGGTCTTTCA AGGATAGAAA GCTAAAGGAA GAGGTGACAT TTTGGATATA TTTTCTATCA 
GATAAAACCT ATATTAGAGT GGGAGATGGG ACACCTTTTT ATCTTCCACT ACGCCAATGT 
CAGCAACGCA TGTTTGCCGT CTACTGTGCG ACAGCAGGTC TTATTCTTCC TTTCTTTCTG 
GTTTCCACGA TCTGTTACAA GATGCTGAAA AAGTCCGACT GTAGCTGCAT AAAACGTAAA 
GCTGTCACCT TTGTCTCAGT ATCAATTATT GCCCTAATTT CAAGCATGGC AGTGGGGAAT 
ATGTTCTTCT TTCTGCCTAC GCATGATTTA CGCACATGCG CGGCACAATT CGCTAACGTC 
ACTCCAACTG CGTTGACCCC ATCCAACGTA ACAGACATAC TCAATATAAC ATTGTTGGAC 
GAAAGCAAAC CAACGATATG TGGACCACAA TCTTGCCATT TTCCACAGTA CTTCACTTAC 
AGCATGGTGC TGTCTATGCT TGCCTGTGCC CAATTTCTTG GTACGTCGGG ATTCTCGAAA 
TTAATTTTGC TTCTCAGCCA GTCCGTGTTG TACACGTTGC TGGTACTTCT GGTAAACCCT 
ACGTTGTTTG ACAATGCTGA TCTGCTGAAC CATGCTACAT TCGAAGAAAG CGTGATGATA 
CCTCTACGTT TGATGTCTCC GTTCATTTTG ATGCTGTTCG TTGCTGCGGT GCTCATGCAA 
GGCCAACAGG CAGAGCTCAC AGCGAGGATG GATTTTCTAT GGAACAAACA GGCTGCTGAA 
GAAAGAGAAG AAATGGTGAA GAAACAAGCT TATAACCGGA AACTTATTCA CAATATCTTG 
CCTGTAGATG TAGCCAAGTA TTATCTTGAT AAGAAAGAAA AAAATGACGA TGATCTTTAC 
TGCCAGTCGT GTGAATGTGT CGCCGTTATG TTCGCAACAA TAACCAATTT CTCTGAGTTT 
TATATGGAAT TAGACGCAAA TAATGAAGGC GTCGAATGCC TGAGATTGCT CAACGAAATT 
ATCGCCGATT TTGACAATAT CATTAGTGAA CCGCAGTTTT ACCAGCTTGA AAAAATCAAA 
ACGATGGGCA GTACATATAT GGTCTCGTCG GGGCTAAATA GCGCAACTTA TGATAAAGAA 
AATATGAGCC ACATTTCAGC ACTCGCGGTT TTTGCCATGC GACTACAAGA CCAGCTGAAA 
TTTGTCAATA CACATTCGTT TAATAACTTC AAATTCAGAA TAGGGTTAAA CTGCGGACCA 
GTGGTTGCAG GGGTAATAGG GGCTCGTAAA CCCCAATATG ATATATGGGG CAATACGGTA 
AACGTTGCAA GTAGAATGGA CAGTACTGGT ATAGACGACA AAATTCAGGT TTCAGAGGAC 
GTTTACAATC TCCTAAAAAC AAAGGGTTTT GATTTTGTTC CTCGCGGGTT TGTGCCTGTT 
AAAGGGAAAG GTACCATGAT GACGTATTTC CTCGAAGGCC CACGGCCCAA CGAGGAACCC 
ACGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC778532 ( XM_018814717.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018670262.1 Ciona intestinalis adenylate cyclase type 5 (LOC778532), mRNA.

Price & Availability↑

CloneID	OCi71861
Clone ID Related Accession (Same CDS sequence)	XM_018814717.1
Accession Version	XM_018814717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	adenylate cyclase type 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020179.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGATTCTAC TCAGCCACGT CGTTTTGCAT AACGATCTAC TAGCCACGAA CATTTACGAA 
GATACAAATA TGCAGAAGGA AGAGAGAAAA GGTCTGCTTC GGAATCACGC TTCACTTAAG 
GAAATGACTA CATGTGAGAG CCCTAACTCA TCTAAGATTG AACAGCTAAA GCATTTAAAA 
GATGCCAACG GGGCAACCCC CTCCCCCCCT TTATCCGAGA GGATATTAAA GCGACTGAAC 
CCGAAAATGA GGAAAGCGGG GTCTTTAGAC GAGAACTTGT CCAATTCCCG CCCTGCTACC 
CCTACAACGG GAAGAAACCA TAAAAAGACC CAAGAACGCA TGGGTTCCGC CCCTGTTCCT 
GCCAATTCCC CTCAAGCCTC CACCAAGAAG AGAAACTTCT CTATCAGGCA TAGGACTTTA 
GACTCTGAGG GTGACACCCC TATGTTACCC ATGCAGTCGT TTTCACCTAT ACAGCAACCT 
GAACCCACCC CGGAAGTGGA ATTAAATTCA CGCTCCCCGA AAATGTTAAC GAAATGCTGC 
TTCTGGTCTT CATGCGCTGA AATGCTGCAC AAATTCATGT CTGGAGTCCG TTTTGAATCC 
TGGAGCTCTG AACGTCTCTA CCAGCGCTAC TTCTTCCATT CCAAGATAAA TATGCTTAGC 
ATACTGCTAT GGTTACTCAA ATCTCTTTGC ATCATTTTAT TTTGTTTTAA ATATTGTACA 
GGACAAAATT GGAACGACAT GGTGTATGGA ATTATTATCA TTGTATTGCT TCTACTGTGT 
ATTGCCTTGC AGGTGATCTT TCTTCTCAAT GTACTTTCCC AGCACACATT ATTCTTGGTG 
TCTTACATCA TACTAGCCAT GGTTGTTATC ATTGATCTAG TGTCAGTTGT ACTTTTAGAG 
CGGTCAAGCT CAAGCTACAT GGTATGGCTC ACTTTGTTCA TGGTATACAT TATGTACACC 
ATGATACCTC TGAACATTCT GGTGGTGTCT GCGGCCAGTT TTATCCTCTC CTGCAGCCAT 
ATGCTTGTCT CCTATGTTTC AAATGGACCC AAGCCCCATT ACCCCAACCA GATTGCTTCC 
GACTTCCTTT TGTTTTTCTG CACCAACGTG GTCGGCATGT GCATTCGTAT AACATCAGAA 
CTTGCTCAGC GAAAAGCTTT TACTGAGACG CGAGAATGTA TCGTCGCTCG TCTTAATCTG 
CAAAGGCAGA ATCTGCACCA GGAAAGGTTG TTACTTTCTG TTCTCCCACG TCACGTTGCT 
ATGGAAATGA AAGGTGACAT TGACCAATCG AAACCCAAGA CACAACAATT CTATAAAATT 
TACATCCAAA AGCACAACAA CGTTAGCATA TTGTACGCTG ATATTGAAGG GTTTACGAAA 
CTTGCTTCAC AATGTCAACC CCAGTGGCTT GTTCGGACAT TAAATCAACT TTTCGCACGG 
TTTGATGCTC TAGCACGGGA CAACAACTGC CTACGAATCA AGATTCTTGG AGATTGCTAT 
TATTGCGTGG CTGGCTTACC CGAGCCTAGA CCTGACCACG CGCATGCTTG TGTACAAATG 
GGCTTGGACA TGATAGACAC AATATCTTTA ATTCGGGAAA TGACCGGCAT ACCAGAATTA 
AACATGAGAG TTGGTATTCA CACTGGTCGT GTACACTGTG GGGTACTTGG TCTCAAAAAA 
TGGCAGTTTG ACGTTTGGAG TAACGATGTC ACACTAGCCA ACCTTATGGA AGCTGGCGGC 
AAAGCAGGGC GCATTCATAT AACTGCAGCT ACACTTAAGT ATTTACACGA CGATTACGAA 
GTGGAACCCG GCTATGGTTA CGAACGCAAC AGTAAACTAC AAGGTGTGGA AACTTACCTC 
ATAGTCAAGC AAAAGCCTCA AGCAAAGTTG CCGACCATAA TGCCGCAGGT CAGTACCAAC 
GAGTCCTCAC TGCCGGGACA ACCAGTTCCA ACACATACCA ACAACACAAG GAGAAATAAG 
AACAAAACAG CCAAGGAAAT GAAATTAATG GGCTTTGAAT ACTCAAAGAA CCTTTCCCAC 
GATTTACTGA AAGACAACAA CAGTATTTTA TCCGACGGCG AAGTAAACGA CTTTTTGAAC 
CGCGCAATTG ACGCACAAGG ACTTGACTTT ATCAAGTCAA ACCAGCTCAG CAAATACTTT 
CGGTCTTTCA AGGATAGAAA GCTAAAGGAA GAGTGCTGTC AAATTTTTAG TCAGCAACGC 
ATGTTTGCCG TCTACTGTGC GACAGCAGGT CTTATTCTTC CTTTCTTTCT GGTTTCCACG 
ATCTGTTACA AGATGCTGAA AAAGTCCGAC TGTAGCTGCA TAAAACGTAA AGCTGTCACC 
TTTGTCTCAG TATCAATTAT TGCCCTAATT TCAAGCATGG CAGTGGGGAA TATGTTCTTC 
TTTCTGCCTA CGCATGATTT ACGCACATGC GCGGCACAAT TCGCTAACGT CACTCCAACT 
GCGTTGACCC CATCCAACGT AACAGACATA CTCAATATAA CATTGTTGGA CGAAAGCAAA 
CCAACGATAT GTGGACCACA ATCTTGCCAT TTTCCACAGT ACTTCACTTA CAGCATGGTG 
CTGTCTATGC TTGCCTGTGC CCAATTTCTT GGTACGTCGG GATTCTCGAA ATTAATTTTG 
CTTCTCAGCC AGTCCGTGTT GTACACGTTG CTGGTACTTC TGGTAAACCC TACGTTGTTT 
GACAATGCTG ATCTGCTGAA CCATGCTACA TTCGAAGAAA GCGTGATGAT ACCTCTACGT 
TTGATGTCTC CGTTCATTTT GATGCTGTTC GTTGCTGCGG TGCTCATGCA AGGCCAACAG 
GCAGAGCTCA CAGCGAGGAT GGATTTTCTA TGGAACAAAC AGGCTGCTGA AGAAAGAGAA 
GAAATGGTGA AGAAACAAGC TTATAACCGG AAACTTATTC ACAATATCTT GCCTGTAGAT 
GTAGCCAAGT ATTATCTTGA TAAGAAAGAA AAAAATGACG ATGATCTTTA CTGCCAGTCG 
TGTGAATGTG TCGCCGTTAT GTTCGCAACA ATAACCAATT TCTCTGAGTT TTATATGGAA 
TTAGACGCAA ATAATGAAGG CGTCGAATGC CTGAGATTGC TCAACGAAAT TATCGCCGAT 
TTTGACAATA TCATTAGTGA ACCGCAGTTT TACCAGCTTG AAAAAATCAA AACGATGGGC 
AGTACATATA TGGTCTCGTC GGGGCTAAAT AGCGCAACTT ATGATAAAGA AAATATGAGC 
CACATTTCAG CACTCGCGGT TTTTGCCATG CGACTACAAG ACCAGCTGAA ATTTGTCAAT 
ACACATTCGT TTAATAACTT CAAATTCAGA ATAGGGTTAA ACTGCGGACC AGTGGTTGCA 
GGGGTAATAG GGGCTCGTAA ACCCCAATAT GATATATGGG GCAATACGGT AAACGTTGCA 
AGTAGAATGG ACAGTACTGG TATAGACGAC AAAATTCAGG TTTCAGAGGA CGTTTACAAT 
CTCCTAAAAA CAAAGGGTTT TGATTTTGTT CCTCGCGGGT TTGTGCCTGT TAAAGGGAAA 
GGTACCATGA TGACGTATTT CCTCGAAGGC CCACGGCCCA ACGAGGAACC CACGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018670262.1
CDS	135..3734
Translation	MILLSHVVLHNDLLATNIYEDTNMQKEERKGLLRNHASLKEMTTCESPNSSKIEQLKHLKDANGATPSPPLSERILKRLNPKMRKAGSLDENLSNSRPATPTTGRNHKKTQERMGSAPVPANSPQASTKKRNFSIRHRTLDSEGDTPMLPMQSFSPIQQPEPTPEVELNSRSPKMLTKCCFWSSCAEMLHKFMSGVRFESWSSERLYQRYFFHSKINMLSILLWLLKSLCIILFCFKYCTGQNWNDMVYGIIIIVLLLLCIALQVIFLLNVLSQHTLFLVSYIILAMVVIIDLVSVVLLERSSSSYMVWLTLFMVYIMYTMIPLNILVVSAASFILSCSHMLVSYVSNGPKPHYPNQIASDFLLFFCTNVVGMCIRITSELAQRKAFTETRECIVARLNLQRQNLHQERLLLSVLPRHVAMEMKGDIDQSKPKTQQFYKIYIQKHNNVSILYADIEGFTKLASQCQPQWLVRTLNQLFARFDALARDNNCLRIKILGDCYYCVAGLPEPRPDHAHACVQMGLDMIDTISLIREMTGIPELNMRVGIHTGRVHCGVLGLKKWQFDVWSNDVTLANLMEAGGKAGRIHITAATLKYLHDDYEVEPGYGYERNSKLQGVETYLIVKQKPQAKLPTIMPQVSTNESSLPGQPVPTHTNNTRRNKNKTAKEMKLMGFEYSKNLSHDLLKDNNSILSDGEVNDFLNRAIDAQGLDFIKSNQLSKYFRSFKDRKLKEECCQIFSQQRMFAVYCATAGLILPFFLVSTICYKMLKKSDCSCIKRKAVTFVSVSIIALISSMAVGNMFFFLPTHDLRTCAAQFANVTPTALTPSNVTDILNITLLDESKPTICGPQSCHFPQYFTYSMVLSMLACAQFLGTSGFSKLILLLSQSVLYTLLVLLVNPTLFDNADLLNHATFEESVMIPLRLMSPFILMLFVAAVLMQGQQAELTARMDFLWNKQAAEEREEMVKKQAYNRKLIHNILPVDVAKYYLDKKEKNDDDLYCQSCECVAVMFATITNFSEFYMELDANNEGVECLRLLNEIIADFDNIISEPQFYQLEKIKTMGSTYMVSSGLNSATYDKENMSHISALAVFAMRLQDQLKFVNTHSFNNFKFRIGLNCGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGIDDKIQVSEDVYNLLKTKGFDFVPRGFVPVKGKGTMMTYFLEGPRPNEEPTS

Target ORF information:

RefSeq Version	XM_018814717.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis adenylate cyclase type 5 (LOC778532), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018814717.1

ORF Insert Sequence:

ATGATTCTAC TCAGCCACGT CGTTTTGCAT AACGATCTAC TAGCCACGAA CATTTACGAA 
GATACAAATA TGCAGAAGGA AGAGAGAAAA GGTCTGCTTC GGAATCACGC TTCACTTAAG 
GAAATGACTA CATGTGAGAG CCCTAACTCA TCTAAGATTG AACAGCTAAA GCATTTAAAA 
GATGCCAACG GGGCAACCCC CTCCCCCCCT TTATCCGAGA GGATATTAAA GCGACTGAAC 
CCGAAAATGA GGAAAGCGGG GTCTTTAGAC GAGAACTTGT CCAATTCCCG CCCTGCTACC 
CCTACAACGG GAAGAAACCA TAAAAAGACC CAAGAACGCA TGGGTTCCGC CCCTGTTCCT 
GCCAATTCCC CTCAAGCCTC CACCAAGAAG AGAAACTTCT CTATCAGGCA TAGGACTTTA 
GACTCTGAGG GTGACACCCC TATGTTACCC ATGCAGTCGT TTTCACCTAT ACAGCAACCT 
GAACCCACCC CGGAAGTGGA ATTAAATTCA CGCTCCCCGA AAATGTTAAC GAAATGCTGC 
TTCTGGTCTT CATGCGCTGA AATGCTGCAC AAATTCATGT CTGGAGTCCG TTTTGAATCC 
TGGAGCTCTG AACGTCTCTA CCAGCGCTAC TTCTTCCATT CCAAGATAAA TATGCTTAGC 
ATACTGCTAT GGTTACTCAA ATCTCTTTGC ATCATTTTAT TTTGTTTTAA ATATTGTACA 
GGACAAAATT GGAACGACAT GGTGTATGGA ATTATTATCA TTGTATTGCT TCTACTGTGT 
ATTGCCTTGC AGGTGATCTT TCTTCTCAAT GTACTTTCCC AGCACACATT ATTCTTGGTG 
TCTTACATCA TACTAGCCAT GGTTGTTATC ATTGATCTAG TGTCAGTTGT ACTTTTAGAG 
CGGTCAAGCT CAAGCTACAT GGTATGGCTC ACTTTGTTCA TGGTATACAT TATGTACACC 
ATGATACCTC TGAACATTCT GGTGGTGTCT GCGGCCAGTT TTATCCTCTC CTGCAGCCAT 
ATGCTTGTCT CCTATGTTTC AAATGGACCC AAGCCCCATT ACCCCAACCA GATTGCTTCC 
GACTTCCTTT TGTTTTTCTG CACCAACGTG GTCGGCATGT GCATTCGTAT AACATCAGAA 
CTTGCTCAGC GAAAAGCTTT TACTGAGACG CGAGAATGTA TCGTCGCTCG TCTTAATCTG 
CAAAGGCAGA ATCTGCACCA GGAAAGGTTG TTACTTTCTG TTCTCCCACG TCACGTTGCT 
ATGGAAATGA AAGGTGACAT TGACCAATCG AAACCCAAGA CACAACAATT CTATAAAATT 
TACATCCAAA AGCACAACAA CGTTAGCATA TTGTACGCTG ATATTGAAGG GTTTACGAAA 
CTTGCTTCAC AATGTCAACC CCAGTGGCTT GTTCGGACAT TAAATCAACT TTTCGCACGG 
TTTGATGCTC TAGCACGGGA CAACAACTGC CTACGAATCA AGATTCTTGG AGATTGCTAT 
TATTGCGTGG CTGGCTTACC CGAGCCTAGA CCTGACCACG CGCATGCTTG TGTACAAATG 
GGCTTGGACA TGATAGACAC AATATCTTTA ATTCGGGAAA TGACCGGCAT ACCAGAATTA 
AACATGAGAG TTGGTATTCA CACTGGTCGT GTACACTGTG GGGTACTTGG TCTCAAAAAA 
TGGCAGTTTG ACGTTTGGAG TAACGATGTC ACACTAGCCA ACCTTATGGA AGCTGGCGGC 
AAAGCAGGGC GCATTCATAT AACTGCAGCT ACACTTAAGT ATTTACACGA CGATTACGAA 
GTGGAACCCG GCTATGGTTA CGAACGCAAC AGTAAACTAC AAGGTGTGGA AACTTACCTC 
ATAGTCAAGC AAAAGCCTCA AGCAAAGTTG CCGACCATAA TGCCGCAGGT CAGTACCAAC 
GAGTCCTCAC TGCCGGGACA ACCAGTTCCA ACACATACCA ACAACACAAG GAGAAATAAG 
AACAAAACAG CCAAGGAAAT GAAATTAATG GGCTTTGAAT ACTCAAAGAA CCTTTCCCAC 
GATTTACTGA AAGACAACAA CAGTATTTTA TCCGACGGCG AAGTAAACGA CTTTTTGAAC 
CGCGCAATTG ACGCACAAGG ACTTGACTTT ATCAAGTCAA ACCAGCTCAG CAAATACTTT 
CGGTCTTTCA AGGATAGAAA GCTAAAGGAA GAGTGCTGTC AAATTTTTAG TCAGCAACGC 
ATGTTTGCCG TCTACTGTGC GACAGCAGGT CTTATTCTTC CTTTCTTTCT GGTTTCCACG 
ATCTGTTACA AGATGCTGAA AAAGTCCGAC TGTAGCTGCA TAAAACGTAA AGCTGTCACC 
TTTGTCTCAG TATCAATTAT TGCCCTAATT TCAAGCATGG CAGTGGGGAA TATGTTCTTC 
TTTCTGCCTA CGCATGATTT ACGCACATGC GCGGCACAAT TCGCTAACGT CACTCCAACT 
GCGTTGACCC CATCCAACGT AACAGACATA CTCAATATAA CATTGTTGGA CGAAAGCAAA 
CCAACGATAT GTGGACCACA ATCTTGCCAT TTTCCACAGT ACTTCACTTA CAGCATGGTG 
CTGTCTATGC TTGCCTGTGC CCAATTTCTT GGTACGTCGG GATTCTCGAA ATTAATTTTG 
CTTCTCAGCC AGTCCGTGTT GTACACGTTG CTGGTACTTC TGGTAAACCC TACGTTGTTT 
GACAATGCTG ATCTGCTGAA CCATGCTACA TTCGAAGAAA GCGTGATGAT ACCTCTACGT 
TTGATGTCTC CGTTCATTTT GATGCTGTTC GTTGCTGCGG TGCTCATGCA AGGCCAACAG 
GCAGAGCTCA CAGCGAGGAT GGATTTTCTA TGGAACAAAC AGGCTGCTGA AGAAAGAGAA 
GAAATGGTGA AGAAACAAGC TTATAACCGG AAACTTATTC ACAATATCTT GCCTGTAGAT 
GTAGCCAAGT ATTATCTTGA TAAGAAAGAA AAAAATGACG ATGATCTTTA CTGCCAGTCG 
TGTGAATGTG TCGCCGTTAT GTTCGCAACA ATAACCAATT TCTCTGAGTT TTATATGGAA 
TTAGACGCAA ATAATGAAGG CGTCGAATGC CTGAGATTGC TCAACGAAAT TATCGCCGAT 
TTTGACAATA TCATTAGTGA ACCGCAGTTT TACCAGCTTG AAAAAATCAA AACGATGGGC 
AGTACATATA TGGTCTCGTC GGGGCTAAAT AGCGCAACTT ATGATAAAGA AAATATGAGC 
CACATTTCAG CACTCGCGGT TTTTGCCATG CGACTACAAG ACCAGCTGAA ATTTGTCAAT 
ACACATTCGT TTAATAACTT CAAATTCAGA ATAGGGTTAA ACTGCGGACC AGTGGTTGCA 
GGGGTAATAG GGGCTCGTAA ACCCCAATAT GATATATGGG GCAATACGGT AAACGTTGCA 
AGTAGAATGG ACAGTACTGG TATAGACGAC AAAATTCAGG TTTCAGAGGA CGTTTACAAT 
CTCCTAAAAA CAAAGGGTTT TGATTTTGTT CCTCGCGGGT TTGTGCCTGT TAAAGGGAAA 
GGTACCATGA TGACGTATTT CCTCGAAGGC CCACGGCCCA ACGAGGAACC CACGAGCTGA