siat4 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	siat4
Entrez Gene ID	445728
Full Name	alpha-2,3-sialyltransferase ST3Gal I
Synonyms	SIAT4B
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009859193.2	XP_009857495.1	alpha-2,3-sialyltransferase ST3Gal I isoform X1
XM_009859193.3	XP_009857495.1	alpha-2,3-sialyltransferase ST3Gal I isoform X1
NM_001032531.1	NP_001027703.1	alpha-2,3-sialyltransferase ST3Gal I

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The evolution of galactose alpha2,3-sialyltransferase: Ciona intestinalis ST3GAL I/II and Takifugu rubripes ST3GAL II sialylate Galbeta1,3GalNAc structures on glycoproteins but not glycolipids.
Lehmann F, Kelm S, Dietz F, von Itzstein M, Tiralongo J
Glycoconjugate journal25(4)323-34(2008 May)

The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach.
Harduin-Lepers A, Mollicone R, Delannoy P, Oriol R
Glycobiology15(8)805-17(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The characterization of the only sialyltransferase gene found in the tunicate Ciona intestinalis and ST3Gal II gene from the bony fish Takifugu rubripes, is reported.
Title: The evolution of galactose alpha2,3-sialyltransferase: Ciona intestinalis ST3GAL I/II and Takifugu rubripes ST3GAL II sialylate Galbeta1,3GalNAc structures on glycoproteins but not glycolipids.

The following siat4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the siat4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCi13445	NM_001032531.1 Latest version!	Ciona intestinalis alpha-2,3-sialyltransferase ST3Gal I (siat4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OCi13674	XM_009859193.3 Latest version!	Ciona intestinalis alpha-2,3-sialyltransferase ST3Gal I (siat4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
OCi13674	XM_009859193.2	Ciona intestinalis alpha-2,3-sialyltransferase ST3Gal I (siat4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

siat4 ( NM_001032531.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> NP_001027703.1 Ciona intestinalis alpha-2,3-sialyltransferase ST3Gal I (siat4), mRNA.

Price & Availability↑

CloneID	OCi13445
Clone ID Related Accession (Same CDS sequence)	NM_001032531.1
Accession Version	NM_001032531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-28
Organism	Ciona intestinalis(vase tunicate)
Product	alpha-2,3-sialyltransferase ST3Gal I
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ626815.1. ##Evidence-Data-START## Transcript exon combination :: AJ626815.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028082, SAMD00028084 [ECO:0000350] ##Evidence-Data-END##

ATGTTAATCA ACTTTAAATT AAGTCGTGTG ATTGCTATGC TGCTCGTAGT AGCAATTTTT 
CTAACCTATA GCTGGCTCTT GCTATGGTCA ACAAAGACTG CATTACAAAC AAACCGGAAA 
AACAAAGCCG GCCAAGATGA GGTTCCGGTT ATAAACGTGA TAAAGGAAGA TAGTTATGTG 
CAGCAGAAAA CTCAAAACCT TAATAAAGGA AAGAGGTTTG ATTTAGGAAG AGTAAATCAT 
TCGCATCCTC GGGAGGAGAT ACAGCAGAAC AATAAATGCG GACATCAACT TGATGCTTCT 
CAAACACGGT GGTTTAGAGC ACGGTTTAAC CCAGAAATTG AACCTGTGTG GACACAGAGC 
GCACTGGAGA TTGATTACTT GGTATATGAC TGGTGGCTAT CGCTGCAGTC AAGCGAAGCA 
GAGAATCTGG ACAAAACGTT CGAAGCATTG TACAAAGAAG GCGTGCCAAG GAAAGATCCA 
TTTGCTAGAC TAACTCATGA CAGAGAGGCT GGCTGTAGAA GTTGTGCTGT GGTTGGAAAC 
TCTGGTAATA TACTCAACTC AAACTATGGA AATGTGATAG ACGGACATGA TTTTGTCATT 
CGTATGAACA AAGGTCCTAC CTATAACTAT GAAAATGATG TGGGTTCAAA AACAACACAC 
AGATTTATGT ATCCTACAAC TGCTGCAAGC TCACTGCCTC AAGGAGTCAG TTTGGTACTG 
GTGCCATTTC AACCACTGGA CATTAAATGG CTTCTCAGTG CTTTAACCAC AGGAGAAATA 
ACAAGGACCT ACCAACCGCT GGTTAGGCGA GTTACATGCG ACAAATCAAA GATTACCATC 
ATTAGTCCTA CATTCATACG CTATGTACAT GACCGATGGA CTCAGCACCA TGGGAGGTAT 
CCCTCCACAG GATTGTTGGC ACTTATATAT GCACTACACG AGTGTGATGA AGTTGATGTG 
TATGGATTTG GAGCTAACAG AGCGGGGAAC TGGCACCATT ACTGGGAGGA CTTGCCCCCC 
CATGTGGCGG GGGCTTTTCG TAAAACTGGC GTCCATGATT CAGCGCAAGA AAATGAGATT 
ATTGACCAGT TACATATACA TGGACTGCTA AGGGTACACA GGTCGGAACA ATCGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027703.1
CDS	1..1140
Translation	MLINFKLSRVIAMLLVVAIFLTYSWLLLWSTKTALQTNRKNKAGQDEVPVINVIKEDSYVQQKTQNLNKGKRFDLGRVNHSHPREEIQQNNKCGHQLDASQTRWFRARFNPEIEPVWTQSALEIDYLVYDWWLSLQSSEAENLDKTFEALYKEGVPRKDPFARLTHDREAGCRSCAVVGNSGNILNSNYGNVIDGHDFVIRMNKGPTYNYENDVGSKTTHRFMYPTTAASSLPQGVSLVLVPFQPLDIKWLLSALTTGEITRTYQPLVRRVTCDKSKITIISPTFIRYVHDRWTQHHGRYPSTGLLALIYALHECDEVDVYGFGANRAGNWHHYWEDLPPHVAGAFRKTGVHDSAQENEIIDQLHIHGLLRVHRSEQSS

Target ORF information:

RefSeq Version	NM_001032531.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis alpha-2,3-sialyltransferase ST3Gal I (siat4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032531.1

ORF Insert Sequence:

ATGTTAATCA ACTTTAAATT AAGTCGTGTG ATTGCTATGC TGCTCGTAGT AGCAATTTTT 
CTAACCTATA GCTGGCTCTT GCTATGGTCA ACAAAGACTG CATTACAAAC AAACCGGAAA 
AACAAAGCCG GCCAAGATGA GGTTCCGGTT ATAAACGTGA TAAAGGAAGA TAGTTATGTG 
CAGCAGAAAA CTCAAAACCT TAATAAAGGA AAGAGGTTTG ATTTAGGAAG AGTAAATCAT 
TCGCATCCTC GGGAGGAGAT ACAGCAGAAC AATAAATGCG GACATCAACT TGATGCTTCT 
CAAACACGGT GGTTTAGAGC ACGGTTTAAC CCAGAAATTG AACCTGTGTG GACACAGAGC 
GCACTGGAGA TTGATTACTT GGTATATGAC TGGTGGCTAT CGCTGCAGTC AAGCGAAGCA 
GAGAATCTGG ACAAAACGTT CGAAGCATTG TACAAAGAAG GCGTGCCAAG GAAAGATCCA 
TTTGCTAGAC TAACTCATGA CAGAGAGGCT GGCTGTAGAA GTTGTGCTGT GGTTGGAAAC 
TCTGGTAATA TACTCAACTC AAACTATGGA AATGTGATAG ACGGACATGA TTTTGTCATT 
CGTATGAACA AAGGTCCTAC CTATAACTAT GAAAATGATG TGGGTTCAAA AACAACACAC 
AGATTTATGT ATCCTACAAC TGCTGCAAGC TCACTGCCTC AAGGAGTCAG TTTGGTACTG 
GTGCCATTTC AACCACTGGA CATTAAATGG CTTCTCAGTG CTTTAACCAC AGGAGAAATA 
ACAAGGACCT ACCAACCGCT GGTTAGGCGA GTTACATGCG ACAAATCAAA GATTACCATC 
ATTAGTCCTA CATTCATACG CTATGTACAT GACCGATGGA CTCAGCACCA TGGGAGGTAT 
CCCTCCACAG GATTGTTGGC ACTTATATAT GCACTACACG AGTGTGATGA AGTTGATGTG 
TATGGATTTG GAGCTAACAG AGCGGGGAAC TGGCACCATT ACTGGGAGGA CTTGCCCCCC 
CATGTGGCGG GGGCTTTTCG TAAAACTGGC GTCCATGATT CAGCGCAAGA AAATGAGATT 
ATTGACCAGT TACATATACA TGGACTGCTA AGGGTACACA GGTCGGAACA ATCGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

siat4 ( XM_009859193.2 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_009857495.1 Ciona intestinalis alpha-2,3-sialyltransferase ST3Gal I (siat4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCi13674
Clone ID Related Accession (Same CDS sequence)	XM_009859193.2 , XM_009859193.3
Accession Version	XM_009859193.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	alpha-2,3-sialyltransferase ST3Gal I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020167.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 21, 2016 this sequence version replaced XM_009859193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAATCA ACTTTAAATT AAGTCGTGTG ATTGCTATGC TGCTCGTAGT AGCAATTTTT 
CTAACCTATA GCTGGCTCTT GCTATGGTCA ACAAAGACTG CATTACAAAC AAACCGGAAA 
AACAAAGCCG GCCAAGATGA GGTTCCGGTT ATAAACGTGA TAAAGGAAGA TAGTTATGTG 
CAGCAGAAAA CTCAAAACCT TAATAAAGGA AAGAGGTTTG ATTTAGGAAG AGTAAATCAT 
TCGCATCCTC GGGAGGAGAT ACAGCAGAAC AATAAATGCG GACATCAACT TGATGCTTCT 
CAAACACGGT GGTTTAGAGC ACGGTTTAAC CCAGAAATTG AACCTGTGTG GACACAGAGC 
GCACTGGAGA TTGATTACTT GGTATATGAC TGGTGGCTAT CGCTGCAGTC AAGCGAAGCA 
GAGAATCTGG ACAAAACGTT CGAAGCATTG TACAAAGAAG GCGTGCCAAG GAAAGATCCA 
TTTGCTAGAC TAACTCATGA CAGAGAGGCT GGCTGTAGAA GTTGTGCTGT GGTTGGAAAC 
TCTGGTAATA TACTCAACTC AAACTATGGA AATGAAATAG ACGGACATGA TTTTGTCATT 
CGTATGAACA AAGGTCCTAC CTATAACTAT GAAAATGATG TGGGTTCAAA AACAACACAC 
AGATTTATGT ATCCTACAAC TGCTGCAAGC TCACTGCCTC AAGGAGTCAG TTTGGTACTG 
GTGCCATTTC AACCACTGGA CATTAAATGG CTTCTCAGTG CTTTAACCAC AGGAGAAATA 
ACAAGGACCT ACCAACCGCT GGTTAGGCGA GTTACATGCG ACAAATCAAA GATTACCATC 
ATTAGTCCTA CATTCATACG CTATGTACAT GACCGATGGA CTCAGCACCA TGGGAGGTAT 
CCCTCCACAG GATTGTTGGC ACTTATATAT GCACTACACG AGTGTGATGA AGTTGATGTG 
TATGGATTTG GAGCTAACAG AGCGGGGAAC TGGCACCATT ACTGGGAGGA CTTGCCCCCC 
CATGTGGCGG GGGCTTTTCG TAAAACTGGC GTCCATGATT CAGCGCAAGA AAATGAGATT 
ATTGACCAGT TACATATACA TGGACTGCTA AGGGTACACA GGTCGGAACA ATCGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009857495.1
CDS	765..1904
Translation	MLINFKLSRVIAMLLVVAIFLTYSWLLLWSTKTALQTNRKNKAGQDEVPVINVIKEDSYVQQKTQNLNKGKRFDLGRVNHSHPREEIQQNNKCGHQLDASQTRWFRARFNPEIEPVWTQSALEIDYLVYDWWLSLQSSEAENLDKTFEALYKEGVPRKDPFARLTHDREAGCRSCAVVGNSGNILNSNYGNEIDGHDFVIRMNKGPTYNYENDVGSKTTHRFMYPTTAASSLPQGVSLVLVPFQPLDIKWLLSALTTGEITRTYQPLVRRVTCDKSKITIISPTFIRYVHDRWTQHHGRYPSTGLLALIYALHECDEVDVYGFGANRAGNWHHYWEDLPPHVAGAFRKTGVHDSAQENEIIDQLHIHGLLRVHRSEQSS

Target ORF information:

RefSeq Version	XM_009859193.2
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis alpha-2,3-sialyltransferase ST3Gal I (siat4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009859193.2

ORF Insert Sequence:

ATGTTAATCA ACTTTAAATT AAGTCGTGTG ATTGCTATGC TGCTCGTAGT AGCAATTTTT 
CTAACCTATA GCTGGCTCTT GCTATGGTCA ACAAAGACTG CATTACAAAC AAACCGGAAA 
AACAAAGCCG GCCAAGATGA GGTTCCGGTT ATAAACGTGA TAAAGGAAGA TAGTTATGTG 
CAGCAGAAAA CTCAAAACCT TAATAAAGGA AAGAGGTTTG ATTTAGGAAG AGTAAATCAT 
TCGCATCCTC GGGAGGAGAT ACAGCAGAAC AATAAATGCG GACATCAACT TGATGCTTCT 
CAAACACGGT GGTTTAGAGC ACGGTTTAAC CCAGAAATTG AACCTGTGTG GACACAGAGC 
GCACTGGAGA TTGATTACTT GGTATATGAC TGGTGGCTAT CGCTGCAGTC AAGCGAAGCA 
GAGAATCTGG ACAAAACGTT CGAAGCATTG TACAAAGAAG GCGTGCCAAG GAAAGATCCA 
TTTGCTAGAC TAACTCATGA CAGAGAGGCT GGCTGTAGAA GTTGTGCTGT GGTTGGAAAC 
TCTGGTAATA TACTCAACTC AAACTATGGA AATGAAATAG ACGGACATGA TTTTGTCATT 
CGTATGAACA AAGGTCCTAC CTATAACTAT GAAAATGATG TGGGTTCAAA AACAACACAC 
AGATTTATGT ATCCTACAAC TGCTGCAAGC TCACTGCCTC AAGGAGTCAG TTTGGTACTG 
GTGCCATTTC AACCACTGGA CATTAAATGG CTTCTCAGTG CTTTAACCAC AGGAGAAATA 
ACAAGGACCT ACCAACCGCT GGTTAGGCGA GTTACATGCG ACAAATCAAA GATTACCATC 
ATTAGTCCTA CATTCATACG CTATGTACAT GACCGATGGA CTCAGCACCA TGGGAGGTAT 
CCCTCCACAG GATTGTTGGC ACTTATATAT GCACTACACG AGTGTGATGA AGTTGATGTG 
TATGGATTTG GAGCTAACAG AGCGGGGAAC TGGCACCATT ACTGGGAGGA CTTGCCCCCC 
CATGTGGCGG GGGCTTTTCG TAAAACTGGC GTCCATGATT CAGCGCAAGA AAATGAGATT 
ATTGACCAGT TACATATACA TGGACTGCTA AGGGTACACA GGTCGGAACA ATCGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

siat4 ( XM_009859193.3 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_009857495.1 Ciona intestinalis alpha-2,3-sialyltransferase ST3Gal I (siat4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCi13674
Clone ID Related Accession (Same CDS sequence)	XM_009859193.2 , XM_009859193.3
Accession Version	XM_009859193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	alpha-2,3-sialyltransferase ST3Gal I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020167.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 22, 2018 this sequence version replaced XM_009859193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAATCA ACTTTAAATT AAGTCGTGTG ATTGCTATGC TGCTCGTAGT AGCAATTTTT 
CTAACCTATA GCTGGCTCTT GCTATGGTCA ACAAAGACTG CATTACAAAC AAACCGGAAA 
AACAAAGCCG GCCAAGATGA GGTTCCGGTT ATAAACGTGA TAAAGGAAGA TAGTTATGTG 
CAGCAGAAAA CTCAAAACCT TAATAAAGGA AAGAGGTTTG ATTTAGGAAG AGTAAATCAT 
TCGCATCCTC GGGAGGAGAT ACAGCAGAAC AATAAATGCG GACATCAACT TGATGCTTCT 
CAAACACGGT GGTTTAGAGC ACGGTTTAAC CCAGAAATTG AACCTGTGTG GACACAGAGC 
GCACTGGAGA TTGATTACTT GGTATATGAC TGGTGGCTAT CGCTGCAGTC AAGCGAAGCA 
GAGAATCTGG ACAAAACGTT CGAAGCATTG TACAAAGAAG GCGTGCCAAG GAAAGATCCA 
TTTGCTAGAC TAACTCATGA CAGAGAGGCT GGCTGTAGAA GTTGTGCTGT GGTTGGAAAC 
TCTGGTAATA TACTCAACTC AAACTATGGA AATGAAATAG ACGGACATGA TTTTGTCATT 
CGTATGAACA AAGGTCCTAC CTATAACTAT GAAAATGATG TGGGTTCAAA AACAACACAC 
AGATTTATGT ATCCTACAAC TGCTGCAAGC TCACTGCCTC AAGGAGTCAG TTTGGTACTG 
GTGCCATTTC AACCACTGGA CATTAAATGG CTTCTCAGTG CTTTAACCAC AGGAGAAATA 
ACAAGGACCT ACCAACCGCT GGTTAGGCGA GTTACATGCG ACAAATCAAA GATTACCATC 
ATTAGTCCTA CATTCATACG CTATGTACAT GACCGATGGA CTCAGCACCA TGGGAGGTAT 
CCCTCCACAG GATTGTTGGC ACTTATATAT GCACTACACG AGTGTGATGA AGTTGATGTG 
TATGGATTTG GAGCTAACAG AGCGGGGAAC TGGCACCATT ACTGGGAGGA CTTGCCCCCC 
CATGTGGCGG GGGCTTTTCG TAAAACTGGC GTCCATGATT CAGCGCAAGA AAATGAGATT 
ATTGACCAGT TACATATACA TGGACTGCTA AGGGTACACA GGTCGGAACA ATCGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009857495.1
CDS	16..1155
Translation	MLINFKLSRVIAMLLVVAIFLTYSWLLLWSTKTALQTNRKNKAGQDEVPVINVIKEDSYVQQKTQNLNKGKRFDLGRVNHSHPREEIQQNNKCGHQLDASQTRWFRARFNPEIEPVWTQSALEIDYLVYDWWLSLQSSEAENLDKTFEALYKEGVPRKDPFARLTHDREAGCRSCAVVGNSGNILNSNYGNEIDGHDFVIRMNKGPTYNYENDVGSKTTHRFMYPTTAASSLPQGVSLVLVPFQPLDIKWLLSALTTGEITRTYQPLVRRVTCDKSKITIISPTFIRYVHDRWTQHHGRYPSTGLLALIYALHECDEVDVYGFGANRAGNWHHYWEDLPPHVAGAFRKTGVHDSAQENEIIDQLHIHGLLRVHRSEQSS

Target ORF information:

RefSeq Version	XM_009859193.3
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis alpha-2,3-sialyltransferase ST3Gal I (siat4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009859193.3

ORF Insert Sequence:

ATGTTAATCA ACTTTAAATT AAGTCGTGTG ATTGCTATGC TGCTCGTAGT AGCAATTTTT 
CTAACCTATA GCTGGCTCTT GCTATGGTCA ACAAAGACTG CATTACAAAC AAACCGGAAA 
AACAAAGCCG GCCAAGATGA GGTTCCGGTT ATAAACGTGA TAAAGGAAGA TAGTTATGTG 
CAGCAGAAAA CTCAAAACCT TAATAAAGGA AAGAGGTTTG ATTTAGGAAG AGTAAATCAT 
TCGCATCCTC GGGAGGAGAT ACAGCAGAAC AATAAATGCG GACATCAACT TGATGCTTCT 
CAAACACGGT GGTTTAGAGC ACGGTTTAAC CCAGAAATTG AACCTGTGTG GACACAGAGC 
GCACTGGAGA TTGATTACTT GGTATATGAC TGGTGGCTAT CGCTGCAGTC AAGCGAAGCA 
GAGAATCTGG ACAAAACGTT CGAAGCATTG TACAAAGAAG GCGTGCCAAG GAAAGATCCA 
TTTGCTAGAC TAACTCATGA CAGAGAGGCT GGCTGTAGAA GTTGTGCTGT GGTTGGAAAC 
TCTGGTAATA TACTCAACTC AAACTATGGA AATGAAATAG ACGGACATGA TTTTGTCATT 
CGTATGAACA AAGGTCCTAC CTATAACTAT GAAAATGATG TGGGTTCAAA AACAACACAC 
AGATTTATGT ATCCTACAAC TGCTGCAAGC TCACTGCCTC AAGGAGTCAG TTTGGTACTG 
GTGCCATTTC AACCACTGGA CATTAAATGG CTTCTCAGTG CTTTAACCAC AGGAGAAATA 
ACAAGGACCT ACCAACCGCT GGTTAGGCGA GTTACATGCG ACAAATCAAA GATTACCATC 
ATTAGTCCTA CATTCATACG CTATGTACAT GACCGATGGA CTCAGCACCA TGGGAGGTAT 
CCCTCCACAG GATTGTTGGC ACTTATATAT GCACTACACG AGTGTGATGA AGTTGATGTG 
TATGGATTTG GAGCTAACAG AGCGGGGAAC TGGCACCATT ACTGGGAGGA CTTGCCCCCC 
CATGTGGCGG GGGCTTTTCG TAAAACTGGC GTCCATGATT CAGCGCAAGA AAATGAGATT 
ATTGACCAGT TACATATACA TGGACTGCTA AGGGTACACA GGTCGGAACA ATCGTCATAG