LOC100179035 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100179035
Entrez Gene ID	100179035
Full Name	uncharacterized LOC100179035
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018814069.1	XP_018669614.1	uncharacterized protein LOC100179035
XM_018814069.2	XP_018669614.2	RGS domain-containing serine/threonine-protein kinase A

Pathways from BioSystems
Gene Ontology

The following LOC100179035 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100179035 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCi153985	XM_018814069.2 Latest version!	Ciona intestinalis RGS domain-containing serine/threonine-protein kinase A (LOC100179035), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCi71271	XM_018814069.1 Latest version!	Ciona intestinalis uncharacterized LOC100179035 (LOC100179035), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100179035 ( XM_018814069.2 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018669614.2 Ciona intestinalis RGS domain-containing serine/threonine-protein kinase A (LOC100179035), mRNA.

Price & Availability↑

CloneID	OCi153985
Clone ID Related Accession (Same CDS sequence)	XM_018814069.2
Accession Version	XM_018814069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	RGS domain-containing serine/threonine-protein kinase A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020176.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 22, 2018 this sequence version replaced XM_018814069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGAAT ATAAACAAGA TGATACGAAG GATTCTAAAA TTGTGAAACA AAACAGTGAG 
GATCAAATTA TAGCGGGGGA AGTATCGGAC ACAATGGAAA ATAATTCCTC AATCTTGTCG 
CCAACAAAAG ATGTTTCAAA CAATGCTGAC GAGCTTTTGT TTGAGGGAGA GTTTATTGAA 
ACAGCAGCGG AGATTGATGT TAAGACACAA CGCTGCTTGT CACGATTCAG CAACATGGGG 
CCTTCTCATA GTGACTACAT CGCCGCAAAT AACATTGTTG CCACAACACC AATAATAGTT 
GAAGCATATC CTGTTCTTGA CGATCACAAA CAACTTTTAC AAAGAGTTTC GGAAACGAAG 
ACTGTCGTTC ATCCTCGCAG GGCCAAAACT GCATTTGAAA AACATAAACC AAATAAATTA 
AAATCAAAAA CAAAAGCGAA TGAAACCAAT AAACGAAAAG TTACTCGTGC AAAATCTGCT 
GGACTTTCTG TTGAGAAAAG AAACAAAAAT CAAAAAAATG AAAATTTAAA AACTTCATAT 
GATTTTGAAA ATTTTGATTC AACCAAACAA GCTTTGATAT CTGGGCAGAC ATGGCATGTT 
GTGGTTGACA AGAATGAAAC TGGAGATCAT ATTATTGTGA ATGGAGACAA TGGAATAGAT 
GCACGTGATG AATCACCATT GTTGCACAAC AATGGGGCAA TCCACGAGCG TAAATTAAGT 
CCGATCCCAG AAACTTCTTT GTCTTCAATG GATAAAGATA AACTCCTGAT GATGCAAGAA 
AATATTCAGA AGTTAAACAA CGACAACAAT AATAATAACA TTGCTACATG TAACAATGAT 
TATGTTGATG TAATAATTGA TGATAATGAG GCAGCTATGA CATCACAAAG GGCAGATAGT 
GTGATGTCAA ATATAATTCT CGCTAACCCA GTCACAACCC CTATGTCTTC AGCTGGAACA 
CCAGTGTTGT CCAAGACCGG CGACGCTTGG TGTAACTGCA ATGCTGAAGT ATTGGGGAAA 
AGTGAGAATG TGTTTACATT TGGAAACACT GAAAATAGTG TTTTTGTGCA TGGAAACACT 
GAAAATAATG AGCTTTTAAA TAATGACGCT GAATATAAAG AGTTTTTAAA TGGAACCACT 
GGAAATGATC AGTTTTTAAA TGGAACCACT GGGAACAAAA ATGTGAATGG CAACACTGGG 
TTTCAATTTC ATCCAGATGA GAATGCAGGA AAAGATGAAT CGGAAGATCA GATCTTTCGG 
AGTCTGGATG CGAGCCAGGA GATCCAGGAA GATCAAGATG AGTTCTTCGT TGAGTTGAGC 
AATTGTTACG ATGGGGATGA TGAGACAGAG GTGGGAAGTG ATGTTGGAAG CGATGGTGGG 
AGCAGTGAAG AGTCTTTTGA TGAGTTGGAG GACTTTAAGA GGAAAATGCT TGCTCCAAGG 
TCGGAGCTGG ATGCTCACAT CCTCCATAGA GCATCAGAAG ACGAGGAAAG TGATGAAGAA 
GATTGTGAGG GCGGTGGTGG GCTGGTCCTC CAGGGTGAAG GGAGTCTTGT GGATCTGGGG 
GGTCCTGGAG AAAGGCAAGA GGGAATGAAG ATTGTTCCAA AATACGATGT TTTATTTGAT 
AATAATGCAG AAGATGTTGG AGGAAATGAG TGTTCGGGGA ATGTTGCGAA ATATAATGTT 
TTGTTCGAGG AGAAAAATTT AGAAGTTGGA AATATTGAGA TGTGTCCGGA GGATGGGGGT 
GGGAATAAAG ATGAGGATGA AGAAGAGGCC TTAGATTGCT CCCAGGAGAG TCCAGGAGCA 
TCAGATGATA ATGGGGATCT AAGTGATGAC GACACAATAT GTGATGAAGA TCAAGGGATG 
AGGAAGGACC ATCTTCATTT ATTGAGAAGA TCGGAATCGC CGGGCGTCTC TCAGGATGAA 
TCGGAGGATG CGATGATGGA TTACATGAGG AAGACACTGA AGGAGGAGGA GCTGGGGACC 
GAGTCGGATG CGAGTGCGGG GGTCAGTGAT CACGCAGAAA ATCTCCATGT TAGCCACAAA 
TTCAATGTAT TAACGGTATC TGCGTTGGAG AGACACAACA GTGAGGTGGA CTTTCTTACA 
ATTCGACAAC AACATGAGAT TAGGAGTTTA TTAGAATCTC GAGACTCCCA GCGAACCGAC 
CACAGTGAAC AAAGTTACTT AACCAACACC TCCTCGCTGT CTGTTGCCGA TCTTAACACT 
GAGAAGTGCA TGGGGTGGAA GAGAGGGAAG CCTATCGGGC AAGGAGCATT TGGAAAGGTA 
TGGGAGGGAA TGACAAACGG GGGGCAACTT ATTGCCGTCA AACAAGTTGA ACTGTCTCCC 
TCCGATCGAG AAAAAGCGAA AAAAGAATTT GAGAATTTGC AGCGAGAGGT TGAGATACTG 
AAGGATATGA AACATACGAA TATTGTTAGT TTCATCGGTA CTTGCTTGGA GGGAAACGTC 
GTGAACATTT TCATGGAGTA TTTAACTGGT GGAACAATAG CTTCGGTGTT GAAGGACTTT 
GGGAACCTGG ACGAGGGCGT GTTTCGGAGA TATACAAAAC AAATACTGGA AGGAGTTTGC 
TACCTTCACA AGCATAATGT GGTGCACAGA GACATAAACG GGAACAACGT GATGCTTCTA 
CCCTGTGGAA CTATTAAGTT AATTGACTTC GGTTGCGCGA AAAAGATCCA TGAGATCAAC 
AACAGTTCAT CGGGGCGTAA CAGTGCCTCT AGTGGTAGAG AGCGACAGTT CAAGTCGGTG 
GTTGGGACCC CATATTGGAT GGCACCCGAG GTCATTAACG GGAAGGCTAA GTACGGACCA 
AAGTCCGATG TTTGGAGCTT GGGATGCACG GTGATAGAGA TGGCCACAGC GAAGCCCCCG 
CTGCATGAGT TGGGAATATA CGGGGCTATG TACCACATCG GGGAAGGTAG ACCGATGCCT 
GTATTATCGA ACAACTTCAC TAAACATGCG AGGTCCTTTG TTATTCAATG CTTAAGAATC 
GATCCATCGT CACGGCCGAC TGCAGACGAA TTGTTACAAC ATAAGTTTAT GCGACATAGA 
AGGTTCGTAA GGAAGTCATT GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018669614.2
CDS	34..3120
Translation	MREYKQDDTKDSKIVKQNSEDQIIAGEVSDTMENNSSILSPTKDVSNNADELLFEGEFIETAAEIDVKTQRCLSRFSNMGPSHSDYIAANNIVATTPIIVEAYPVLDDHKQLLQRVSETKTVVHPRRAKTAFEKHKPNKLKSKTKANETNKRKVTRAKSAGLSVEKRNKNQKNENLKTSYDFENFDSTKQALISGQTWHVVVDKNETGDHIIVNGDNGIDARDESPLLHNNGAIHERKLSPIPETSLSSMDKDKLLMMQENIQKLNNDNNNNNIATCNNDYVDVIIDDNEAAMTSQRADSVMSNIILANPVTTPMSSAGTPVLSKTGDAWCNCNAEVLGKSENVFTFGNTENSVFVHGNTENNELLNNDAEYKEFLNGTTGNDQFLNGTTGNKNVNGNTGFQFHPDENAGKDESEDQIFRSLDASQEIQEDQDEFFVELSNCYDGDDETEVGSDVGSDGGSSEESFDELEDFKRKMLAPRSELDAHILHRASEDEESDEEDCEGGGGLVLQGEGSLVDLGGPGERQEGMKIVPKYDVLFDNNAEDVGGNECSGNVAKYNVLFEEKNLEVGNIEMCPEDGGGNKDEDEEEALDCSQESPGASDDNGDLSDDDTICDEDQGMRKDHLHLLRRSESPGVSQDESEDAMMDYMRKTLKEEELGTESDASAGVSDHAENLHVSHKFNVLTVSALERHNSEVDFLTIRQQHEIRSLLESRDSQRTDHSEQSYLTNTSSLSVADLNTEKCMGWKRGKPIGQGAFGKVWEGMTNGGQLIAVKQVELSPSDREKAKKEFENLQREVEILKDMKHTNIVSFIGTCLEGNVVNIFMEYLTGGTIASVLKDFGNLDEGVFRRYTKQILEGVCYLHKHNVVHRDINGNNVMLLPCGTIKLIDFGCAKKIHEINNSSSGRNSASSGRERQFKSVVGTPYWMAPEVINGKAKYGPKSDVWSLGCTVIEMATAKPPLHELGIYGAMYHIGEGRPMPVLSNNFTKHARSFVIQCLRIDPSSRPTADELLQHKFMRHRRFVRKSLE

Target ORF information:

RefSeq Version	XM_018814069.2
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis RGS domain-containing serine/threonine-protein kinase A (LOC100179035), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018814069.2

ORF Insert Sequence:

ATGCGTGAAT ATAAACAAGA TGATACGAAG GATTCTAAAA TTGTGAAACA AAACAGTGAG 
GATCAAATTA TAGCGGGGGA AGTATCGGAC ACAATGGAAA ATAATTCCTC AATCTTGTCG 
CCAACAAAAG ATGTTTCAAA CAATGCTGAC GAGCTTTTGT TTGAGGGAGA GTTTATTGAA 
ACAGCAGCGG AGATTGATGT TAAGACACAA CGCTGCTTGT CACGATTCAG CAACATGGGG 
CCTTCTCATA GTGACTACAT CGCCGCAAAT AACATTGTTG CCACAACACC AATAATAGTT 
GAAGCATATC CTGTTCTTGA CGATCACAAA CAACTTTTAC AAAGAGTTTC GGAAACGAAG 
ACTGTCGTTC ATCCTCGCAG GGCCAAAACT GCATTTGAAA AACATAAACC AAATAAATTA 
AAATCAAAAA CAAAAGCGAA TGAAACCAAT AAACGAAAAG TTACTCGTGC AAAATCTGCT 
GGACTTTCTG TTGAGAAAAG AAACAAAAAT CAAAAAAATG AAAATTTAAA AACTTCATAT 
GATTTTGAAA ATTTTGATTC AACCAAACAA GCTTTGATAT CTGGGCAGAC ATGGCATGTT 
GTGGTTGACA AGAATGAAAC TGGAGATCAT ATTATTGTGA ATGGAGACAA TGGAATAGAT 
GCACGTGATG AATCACCATT GTTGCACAAC AATGGGGCAA TCCACGAGCG TAAATTAAGT 
CCGATCCCAG AAACTTCTTT GTCTTCAATG GATAAAGATA AACTCCTGAT GATGCAAGAA 
AATATTCAGA AGTTAAACAA CGACAACAAT AATAATAACA TTGCTACATG TAACAATGAT 
TATGTTGATG TAATAATTGA TGATAATGAG GCAGCTATGA CATCACAAAG GGCAGATAGT 
GTGATGTCAA ATATAATTCT CGCTAACCCA GTCACAACCC CTATGTCTTC AGCTGGAACA 
CCAGTGTTGT CCAAGACCGG CGACGCTTGG TGTAACTGCA ATGCTGAAGT ATTGGGGAAA 
AGTGAGAATG TGTTTACATT TGGAAACACT GAAAATAGTG TTTTTGTGCA TGGAAACACT 
GAAAATAATG AGCTTTTAAA TAATGACGCT GAATATAAAG AGTTTTTAAA TGGAACCACT 
GGAAATGATC AGTTTTTAAA TGGAACCACT GGGAACAAAA ATGTGAATGG CAACACTGGG 
TTTCAATTTC ATCCAGATGA GAATGCAGGA AAAGATGAAT CGGAAGATCA GATCTTTCGG 
AGTCTGGATG CGAGCCAGGA GATCCAGGAA GATCAAGATG AGTTCTTCGT TGAGTTGAGC 
AATTGTTACG ATGGGGATGA TGAGACAGAG GTGGGAAGTG ATGTTGGAAG CGATGGTGGG 
AGCAGTGAAG AGTCTTTTGA TGAGTTGGAG GACTTTAAGA GGAAAATGCT TGCTCCAAGG 
TCGGAGCTGG ATGCTCACAT CCTCCATAGA GCATCAGAAG ACGAGGAAAG TGATGAAGAA 
GATTGTGAGG GCGGTGGTGG GCTGGTCCTC CAGGGTGAAG GGAGTCTTGT GGATCTGGGG 
GGTCCTGGAG AAAGGCAAGA GGGAATGAAG ATTGTTCCAA AATACGATGT TTTATTTGAT 
AATAATGCAG AAGATGTTGG AGGAAATGAG TGTTCGGGGA ATGTTGCGAA ATATAATGTT 
TTGTTCGAGG AGAAAAATTT AGAAGTTGGA AATATTGAGA TGTGTCCGGA GGATGGGGGT 
GGGAATAAAG ATGAGGATGA AGAAGAGGCC TTAGATTGCT CCCAGGAGAG TCCAGGAGCA 
TCAGATGATA ATGGGGATCT AAGTGATGAC GACACAATAT GTGATGAAGA TCAAGGGATG 
AGGAAGGACC ATCTTCATTT ATTGAGAAGA TCGGAATCGC CGGGCGTCTC TCAGGATGAA 
TCGGAGGATG CGATGATGGA TTACATGAGG AAGACACTGA AGGAGGAGGA GCTGGGGACC 
GAGTCGGATG CGAGTGCGGG GGTCAGTGAT CACGCAGAAA ATCTCCATGT TAGCCACAAA 
TTCAATGTAT TAACGGTATC TGCGTTGGAG AGACACAACA GTGAGGTGGA CTTTCTTACA 
ATTCGACAAC AACATGAGAT TAGGAGTTTA TTAGAATCTC GAGACTCCCA GCGAACCGAC 
CACAGTGAAC AAAGTTACTT AACCAACACC TCCTCGCTGT CTGTTGCCGA TCTTAACACT 
GAGAAGTGCA TGGGGTGGAA GAGAGGGAAG CCTATCGGGC AAGGAGCATT TGGAAAGGTA 
TGGGAGGGAA TGACAAACGG GGGGCAACTT ATTGCCGTCA AACAAGTTGA ACTGTCTCCC 
TCCGATCGAG AAAAAGCGAA AAAAGAATTT GAGAATTTGC AGCGAGAGGT TGAGATACTG 
AAGGATATGA AACATACGAA TATTGTTAGT TTCATCGGTA CTTGCTTGGA GGGAAACGTC 
GTGAACATTT TCATGGAGTA TTTAACTGGT GGAACAATAG CTTCGGTGTT GAAGGACTTT 
GGGAACCTGG ACGAGGGCGT GTTTCGGAGA TATACAAAAC AAATACTGGA AGGAGTTTGC 
TACCTTCACA AGCATAATGT GGTGCACAGA GACATAAACG GGAACAACGT GATGCTTCTA 
CCCTGTGGAA CTATTAAGTT AATTGACTTC GGTTGCGCGA AAAAGATCCA TGAGATCAAC 
AACAGTTCAT CGGGGCGTAA CAGTGCCTCT AGTGGTAGAG AGCGACAGTT CAAGTCGGTG 
GTTGGGACCC CATATTGGAT GGCACCCGAG GTCATTAACG GGAAGGCTAA GTACGGACCA 
AAGTCCGATG TTTGGAGCTT GGGATGCACG GTGATAGAGA TGGCCACAGC GAAGCCCCCG 
CTGCATGAGT TGGGAATATA CGGGGCTATG TACCACATCG GGGAAGGTAG ACCGATGCCT 
GTATTATCGA ACAACTTCAC TAAACATGCG AGGTCCTTTG TTATTCAATG CTTAAGAATC 
GATCCATCGT CACGGCCGAC TGCAGACGAA TTGTTACAAC ATAAGTTTAT GCGACATAGA 
AGGTTCGTAA GGAAGTCATT GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100179035 ( XM_018814069.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018669614.1 Ciona intestinalis uncharacterized LOC100179035 (LOC100179035), mRNA.

Price & Availability↑

CloneID	OCi71271
Clone ID Related Accession (Same CDS sequence)	XM_018814069.1
Accession Version	XM_018814069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	uncharacterized protein LOC100179035
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020176.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAG TGATCACCCT TCCTCTCCTC CTCAAGAGCG GAAGCAGCAG TTCTTCTCAT 
GGGTCTTATA AGAAGAAGAA AAAAGATAAA ACCTCGCATC CAGTTGATGG AGCAGTAGTT 
GGGAGTGTTC TCACATCAGG GATTTCCCCT CCCCTTGTGG ATAATACTGA CCATGTAGAC 
CCAACAAGGA AAGTGATCGA TTTTGAAAGT CTTGCAACTA AAGTTTCAGA TGAAGTTGAT 
GGTATGTATC GTTTCAATGC TGAGCCTAAC GTGCCCGAAA ATCGATTGAA AGTTAAGAAC 
AAAAGCAGGT CGTCGGCGTC TGCTGTTCTT GTTCCCGCAC TTAGGGTGGA GAGATTTCAG 
CGGAAGAAAT TATCGCAAAG TGCAACACCC AGAGTTGGAG TGAATGATCG GGTTATGGGG 
GTTGGGATGA AACTGGATGA AAAACCAAAA GATATTTATC CCTTTGTTGG AAAGGAAAGT 
TTGTTAAATA AAATGGAGAG TTTAACACCG GATATTGGAA GGAAAGAAAT GGTTTCTGTA 
GCTCGTAATA ATGTGGATTT CTACTTGCCC AAGGAGAATG AACAAGAAGA AAATAAAGAT 
TTAAAACGGA AACATAAAAC AGTTGAATCT GAAGTGGTTT CTGTTAATAA ACAGAATAAT 
AAAGACGGTT TGAAAACAAA GGGGCAAGAA ATAGATCCAC AGAAACCAGA GATACAAATA 
TTGTCCTCAG TGCCGGAGAA AGTTGATAAA CCATCGTCGA TTAATTTGTT TGTACCTGAG 
CAGGATGACG CGGTAAAAAT AACAGTAACA CTGACTGATG ACGTGGATTC GAAAAGCGAG 
GCAAAGCTAG TTGGGGTGGG GAGAACCAAA ACTAAGGTTT TGTCGGCGAA AGCAGCTGAA 
GCAACCAACA GGTTGTATAA ATCTAATGAT GGAAAATCAA AGGTGGATTT GAAGGGAGAG 
AAAAAGGTAG TGAAGTCAAG TAAAGCAAAG TTGAAACCAG CAGATAAAAG TAAAGAACGA 
AAAAGCAGCG CTACGGCAAA ATTTGGTACA AAGAAATTAA AAGGAAATAA AAATCAGAAT 
TTAAACACGA AAAAGAAAAG AAAAGGAGTT TTATCCGAAG GGGTTGTGAT TGCAACAGAT 
GAGACAATGC GTGAATATAA ACAAGATGAT ACGAAGGATT CTAAAATTGT GAAACAAAAC 
AGTGAGGATC AAATTATAGC GGGGGAAGTA TCGGACACAA TGGAAAATAA TTCCTCAATC 
TTGTCGCCAA CAAAAGATGT TTCAAACAAT GCTGACGAGC TTTTGTTTGA GGGAGAGTTT 
ATTGAAACAG CAGCGGAGAT TGATGTTAAG ACACAACGCT GCTTGTCACG ATTCAGCAAC 
ATGGGGCCTT CTCATAGTGA CTACATCGCC GCAAATAACA TTGTTGCCAC AACACCAATA 
ATAGTTGAAG CATATCCTGT TCTTGACGAT CACAAACAAC TTTTACAAAG AGTTTCGGAA 
ACGAAGACTG TCGTTCATCC TCGCAGGGCC AAAACTGCAT TTGAAAAACA TAAACCAAAT 
AAATTAAAAT CAAAAACAAA AGCGAATGAA ACCAATAAAC GAAAAGTTAC TCGTGCAAAA 
TCTGCTGGAC TTTCTGTTGA GAAAAGAAAC AAAAATCAAA AAAATGAAAA TTTAAAAACT 
TCATATGATT TTGAAAATTT TGATTCAACC AAACAAGCTT TGATATCTGG GCAGACATGG 
CATGTTGTGG TTGACAAGAA TGAAACTGGA GATCATATTA TTGTGAATGG AGACAATGGA 
ATAGATGCAC GTGATGAATC ACCATTGTTG CACAACAATG GGGCAATCCA CGAGCGTAAA 
TTAAGTCCGA TCCCAGAAAC TTCTTTGTCT TCAATGGATA AAGATAAACT CCTGATGATG 
CAAGAAAATA TTCAGAAGTT AAACAACGAC AACAATAATA ATAACATTGC TACATGTAAC 
AATGATTATG TTGATGTAAT AATTGATGAT AATGAGGCAG CTATGACATC ACAAAGGGCA 
GATAGTGTGA TGTCAAATAT AATTCTCGCT AACCCAGTCA CAACCCCTAT GTCTTCAGCT 
GGAACACCAG TGTTGTCCAA GACCGGCGAC GCTTGGTGTA ACTGCAATGC TGAAGTATTG 
GGGAAAAGTG AGAATGTGTT TACATTTGGA AACACTGAAA ATAGTGTTTT TGTGCATGGA 
AACACTGAAA ATAATGAGCT TTTAAATAAT GACGCTGAAT ATAAAGAGTT TTTAAATGGA 
ACCACTGGAA ATGATCAGTT TTTAAATGGA ACCACTGGGA ACAAAAATGT GAATGGCAAC 
ACTGGGTTTC AATTTCATCC AGATGAGAAT GCAGGAAAAG ATGAATCGGA AGATCAGATC 
TTTCGGAGTC TGGATGCGAG CCAGGAGATC CAGGAAGATC AAGATGAGTT CTTCGTTGAG 
TTGAGCAATT GTTACGATGG GGATGATGAG ACAGAGGTGG GAAGTGATGT TGGAAGCGAT 
GGTGGGAGCA GTGAAGAGTC TTTTGATGAG TTGGAGGACT TTAAGAGGAA AATGCTTGCT 
CCAAGGTCGG AGCTGGATGC TCACATCCTC CATAGAGCAT CAGAAGACGA GGAAAGTGAT 
GAAGAAGATT GTGAGGGCGG TGGTGGGCTG GTCCTCCAGG GTGAAGGGAG TCTTGTGGAT 
CTGGGGGGTC CTGGAGAAAG GCAAGAGGGA ATGAAGATTG TTCCAAAATA CGATGTTTTA 
TTTGATAATA ATGCAGAAGA TGTTGGAGGA AATGAGTGTT CGGGGAATGT TGCGAAATAT 
AATGTTTTGT TCGAGGAGAA AAATTTAGAA GTTGGAAATA TTGAGATGTG TCCGGAGGAT 
GGGGGTGGGA ATAAAGATGA GGATGAAGAA GAGGCCTTAG ATTGCTCCCA GGAGAGTCCA 
GGAGCATCAG ATGATAATGG GGATCTAAGT GATGACGACA CAATATGTGA TGAAGATCAA 
GGGATGAGGA AGGACCATCT TCATTTATTG AGAAGATCGG AATCGCCGGG CGTCTCTCAG 
GATGAATCGG AGGATGCGAT GATGGATTAC ATGAGGAAGA CACTGAAGGA GGAGGAGCTG 
GGGACCGAGT CGGATGCGAG TGCGGGGGTC AGTGATCACG CAGAAAATCT CCATGTTAGC 
CACAAATTCA ATGTATTAAC GGTATCTGCG TTGGAGAGAC ACAACAGTGA GGTGGACTTT 
CTTACAATTC GACAACAACA TGAGATTAGG AGTTTATTAG AATCTCGAGA CTCCCAGCGA 
ACCGACCACA GTGAACAAAG TTACTTAACC AACACCTCCT CGCTGTCTGT TGCCGATCTT 
AACACTGAGA AGTGCATGGG GTGGAAGAGA GGGAAGCCTA TCGGGCAAGG AGCATTTGGA 
AAGGTATGGG AGGGAATGAC AAACGGGGGG CAACTTATTG CCGTCAAACA AGTTGAACTG 
TCTCCCTCCG ATCGAGAAAA AGCGAAAAAA GAATTTGAGA ATTTGCAGCG AGAGGTTGAG 
ATACTGAAGG ATATGAAACA TACGAATATT GTTAGTTTCA TCGGTACTTG CTTGGAGGGA 
AACGTCGTGA ACATTTTCAT GGAGTATTTA ACTGGTGGAA CAATAGCTTC GGTGTTGAAG 
GACTTTGGGA ACCTGGACGA GGGCGTGTTT CGGAGATATA CAAAACAAAT ACTGGAAGGA 
GTTTGCTACC TTCACAAGCA TAATGTGGTG CACAGAGACA TAAACGGGAA CAACGTGATG 
CTTCTACCCT GTGGAACTAT TAAGTTAATT GACTTCGGTT GCGCGAAAAA GATCCATGAG 
ATCAACAACA GTTCATCGGG GCGTAACAGT GCCTCTAGTG GTAGAGAGCG ACAGTTCAAG 
TCGGTGGTTG GGACCCCATA TTGGATGGCA CCCGAGGTCA TTAACGGGAA GGCTAAGTAC 
GGACCAAAGT CCGATGTTTG GAGCTTGGGA TGCACGGTGA TAGAGATGGC CACAGCGAAG 
CCCCCGCTGC ATGAGTTGGG AATATACGGG GCTATGTACC ACATCGGGGA AGGTAGACCG 
ATGCCTGTAT TATCGAACAA CTTCACTAAA CATGCGAGGT CCTTTGTTAT TCAATGCTTA 
AGAATCGATC CATCGTCACG GCCGACTGCA GACGAATTGT TACAACATAA GTTTATGCGA 
CATAGAAGGT TCGTAAGGAA GTCATTGGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018669614.1
CDS	89..4321
Translation	MGEVITLPLLLKSGSSSSSHGSYKKKKKDKTSHPVDGAVVGSVLTSGISPPLVDNTDHVDPTRKVIDFESLATKVSDEVDGMYRFNAEPNVPENRLKVKNKSRSSASAVLVPALRVERFQRKKLSQSATPRVGVNDRVMGVGMKLDEKPKDIYPFVGKESLLNKMESLTPDIGRKEMVSVARNNVDFYLPKENEQEENKDLKRKHKTVESEVVSVNKQNNKDGLKTKGQEIDPQKPEIQILSSVPEKVDKPSSINLFVPEQDDAVKITVTLTDDVDSKSEAKLVGVGRTKTKVLSAKAAEATNRLYKSNDGKSKVDLKGEKKVVKSSKAKLKPADKSKERKSSATAKFGTKKLKGNKNQNLNTKKKRKGVLSEGVVIATDETMREYKQDDTKDSKIVKQNSEDQIIAGEVSDTMENNSSILSPTKDVSNNADELLFEGEFIETAAEIDVKTQRCLSRFSNMGPSHSDYIAANNIVATTPIIVEAYPVLDDHKQLLQRVSETKTVVHPRRAKTAFEKHKPNKLKSKTKANETNKRKVTRAKSAGLSVEKRNKNQKNENLKTSYDFENFDSTKQALISGQTWHVVVDKNETGDHIIVNGDNGIDARDESPLLHNNGAIHERKLSPIPETSLSSMDKDKLLMMQENIQKLNNDNNNNNIATCNNDYVDVIIDDNEAAMTSQRADSVMSNIILANPVTTPMSSAGTPVLSKTGDAWCNCNAEVLGKSENVFTFGNTENSVFVHGNTENNELLNNDAEYKEFLNGTTGNDQFLNGTTGNKNVNGNTGFQFHPDENAGKDESEDQIFRSLDASQEIQEDQDEFFVELSNCYDGDDETEVGSDVGSDGGSSEESFDELEDFKRKMLAPRSELDAHILHRASEDEESDEEDCEGGGGLVLQGEGSLVDLGGPGERQEGMKIVPKYDVLFDNNAEDVGGNECSGNVAKYNVLFEEKNLEVGNIEMCPEDGGGNKDEDEEEALDCSQESPGASDDNGDLSDDDTICDEDQGMRKDHLHLLRRSESPGVSQDESEDAMMDYMRKTLKEEELGTESDASAGVSDHAENLHVSHKFNVLTVSALERHNSEVDFLTIRQQHEIRSLLESRDSQRTDHSEQSYLTNTSSLSVADLNTEKCMGWKRGKPIGQGAFGKVWEGMTNGGQLIAVKQVELSPSDREKAKKEFENLQREVEILKDMKHTNIVSFIGTCLEGNVVNIFMEYLTGGTIASVLKDFGNLDEGVFRRYTKQILEGVCYLHKHNVVHRDINGNNVMLLPCGTIKLIDFGCAKKIHEINNSSSGRNSASSGRERQFKSVVGTPYWMAPEVINGKAKYGPKSDVWSLGCTVIEMATAKPPLHELGIYGAMYHIGEGRPMPVLSNNFTKHARSFVIQCLRIDPSSRPTADELLQHKFMRHRRFVRKSLE

Target ORF information:

RefSeq Version	XM_018814069.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis uncharacterized LOC100179035 (LOC100179035), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018814069.1

ORF Insert Sequence:

ATGGGGGAAG TGATCACCCT TCCTCTCCTC CTCAAGAGCG GAAGCAGCAG TTCTTCTCAT 
GGGTCTTATA AGAAGAAGAA AAAAGATAAA ACCTCGCATC CAGTTGATGG AGCAGTAGTT 
GGGAGTGTTC TCACATCAGG GATTTCCCCT CCCCTTGTGG ATAATACTGA CCATGTAGAC 
CCAACAAGGA AAGTGATCGA TTTTGAAAGT CTTGCAACTA AAGTTTCAGA TGAAGTTGAT 
GGTATGTATC GTTTCAATGC TGAGCCTAAC GTGCCCGAAA ATCGATTGAA AGTTAAGAAC 
AAAAGCAGGT CGTCGGCGTC TGCTGTTCTT GTTCCCGCAC TTAGGGTGGA GAGATTTCAG 
CGGAAGAAAT TATCGCAAAG TGCAACACCC AGAGTTGGAG TGAATGATCG GGTTATGGGG 
GTTGGGATGA AACTGGATGA AAAACCAAAA GATATTTATC CCTTTGTTGG AAAGGAAAGT 
TTGTTAAATA AAATGGAGAG TTTAACACCG GATATTGGAA GGAAAGAAAT GGTTTCTGTA 
GCTCGTAATA ATGTGGATTT CTACTTGCCC AAGGAGAATG AACAAGAAGA AAATAAAGAT 
TTAAAACGGA AACATAAAAC AGTTGAATCT GAAGTGGTTT CTGTTAATAA ACAGAATAAT 
AAAGACGGTT TGAAAACAAA GGGGCAAGAA ATAGATCCAC AGAAACCAGA GATACAAATA 
TTGTCCTCAG TGCCGGAGAA AGTTGATAAA CCATCGTCGA TTAATTTGTT TGTACCTGAG 
CAGGATGACG CGGTAAAAAT AACAGTAACA CTGACTGATG ACGTGGATTC GAAAAGCGAG 
GCAAAGCTAG TTGGGGTGGG GAGAACCAAA ACTAAGGTTT TGTCGGCGAA AGCAGCTGAA 
GCAACCAACA GGTTGTATAA ATCTAATGAT GGAAAATCAA AGGTGGATTT GAAGGGAGAG 
AAAAAGGTAG TGAAGTCAAG TAAAGCAAAG TTGAAACCAG CAGATAAAAG TAAAGAACGA 
AAAAGCAGCG CTACGGCAAA ATTTGGTACA AAGAAATTAA AAGGAAATAA AAATCAGAAT 
TTAAACACGA AAAAGAAAAG AAAAGGAGTT TTATCCGAAG GGGTTGTGAT TGCAACAGAT 
GAGACAATGC GTGAATATAA ACAAGATGAT ACGAAGGATT CTAAAATTGT GAAACAAAAC 
AGTGAGGATC AAATTATAGC GGGGGAAGTA TCGGACACAA TGGAAAATAA TTCCTCAATC 
TTGTCGCCAA CAAAAGATGT TTCAAACAAT GCTGACGAGC TTTTGTTTGA GGGAGAGTTT 
ATTGAAACAG CAGCGGAGAT TGATGTTAAG ACACAACGCT GCTTGTCACG ATTCAGCAAC 
ATGGGGCCTT CTCATAGTGA CTACATCGCC GCAAATAACA TTGTTGCCAC AACACCAATA 
ATAGTTGAAG CATATCCTGT TCTTGACGAT CACAAACAAC TTTTACAAAG AGTTTCGGAA 
ACGAAGACTG TCGTTCATCC TCGCAGGGCC AAAACTGCAT TTGAAAAACA TAAACCAAAT 
AAATTAAAAT CAAAAACAAA AGCGAATGAA ACCAATAAAC GAAAAGTTAC TCGTGCAAAA 
TCTGCTGGAC TTTCTGTTGA GAAAAGAAAC AAAAATCAAA AAAATGAAAA TTTAAAAACT 
TCATATGATT TTGAAAATTT TGATTCAACC AAACAAGCTT TGATATCTGG GCAGACATGG 
CATGTTGTGG TTGACAAGAA TGAAACTGGA GATCATATTA TTGTGAATGG AGACAATGGA 
ATAGATGCAC GTGATGAATC ACCATTGTTG CACAACAATG GGGCAATCCA CGAGCGTAAA 
TTAAGTCCGA TCCCAGAAAC TTCTTTGTCT TCAATGGATA AAGATAAACT CCTGATGATG 
CAAGAAAATA TTCAGAAGTT AAACAACGAC AACAATAATA ATAACATTGC TACATGTAAC 
AATGATTATG TTGATGTAAT AATTGATGAT AATGAGGCAG CTATGACATC ACAAAGGGCA 
GATAGTGTGA TGTCAAATAT AATTCTCGCT AACCCAGTCA CAACCCCTAT GTCTTCAGCT 
GGAACACCAG TGTTGTCCAA GACCGGCGAC GCTTGGTGTA ACTGCAATGC TGAAGTATTG 
GGGAAAAGTG AGAATGTGTT TACATTTGGA AACACTGAAA ATAGTGTTTT TGTGCATGGA 
AACACTGAAA ATAATGAGCT TTTAAATAAT GACGCTGAAT ATAAAGAGTT TTTAAATGGA 
ACCACTGGAA ATGATCAGTT TTTAAATGGA ACCACTGGGA ACAAAAATGT GAATGGCAAC 
ACTGGGTTTC AATTTCATCC AGATGAGAAT GCAGGAAAAG ATGAATCGGA AGATCAGATC 
TTTCGGAGTC TGGATGCGAG CCAGGAGATC CAGGAAGATC AAGATGAGTT CTTCGTTGAG 
TTGAGCAATT GTTACGATGG GGATGATGAG ACAGAGGTGG GAAGTGATGT TGGAAGCGAT 
GGTGGGAGCA GTGAAGAGTC TTTTGATGAG TTGGAGGACT TTAAGAGGAA AATGCTTGCT 
CCAAGGTCGG AGCTGGATGC TCACATCCTC CATAGAGCAT CAGAAGACGA GGAAAGTGAT 
GAAGAAGATT GTGAGGGCGG TGGTGGGCTG GTCCTCCAGG GTGAAGGGAG TCTTGTGGAT 
CTGGGGGGTC CTGGAGAAAG GCAAGAGGGA ATGAAGATTG TTCCAAAATA CGATGTTTTA 
TTTGATAATA ATGCAGAAGA TGTTGGAGGA AATGAGTGTT CGGGGAATGT TGCGAAATAT 
AATGTTTTGT TCGAGGAGAA AAATTTAGAA GTTGGAAATA TTGAGATGTG TCCGGAGGAT 
GGGGGTGGGA ATAAAGATGA GGATGAAGAA GAGGCCTTAG ATTGCTCCCA GGAGAGTCCA 
GGAGCATCAG ATGATAATGG GGATCTAAGT GATGACGACA CAATATGTGA TGAAGATCAA 
GGGATGAGGA AGGACCATCT TCATTTATTG AGAAGATCGG AATCGCCGGG CGTCTCTCAG 
GATGAATCGG AGGATGCGAT GATGGATTAC ATGAGGAAGA CACTGAAGGA GGAGGAGCTG 
GGGACCGAGT CGGATGCGAG TGCGGGGGTC AGTGATCACG CAGAAAATCT CCATGTTAGC 
CACAAATTCA ATGTATTAAC GGTATCTGCG TTGGAGAGAC ACAACAGTGA GGTGGACTTT 
CTTACAATTC GACAACAACA TGAGATTAGG AGTTTATTAG AATCTCGAGA CTCCCAGCGA 
ACCGACCACA GTGAACAAAG TTACTTAACC AACACCTCCT CGCTGTCTGT TGCCGATCTT 
AACACTGAGA AGTGCATGGG GTGGAAGAGA GGGAAGCCTA TCGGGCAAGG AGCATTTGGA 
AAGGTATGGG AGGGAATGAC AAACGGGGGG CAACTTATTG CCGTCAAACA AGTTGAACTG 
TCTCCCTCCG ATCGAGAAAA AGCGAAAAAA GAATTTGAGA ATTTGCAGCG AGAGGTTGAG 
ATACTGAAGG ATATGAAACA TACGAATATT GTTAGTTTCA TCGGTACTTG CTTGGAGGGA 
AACGTCGTGA ACATTTTCAT GGAGTATTTA ACTGGTGGAA CAATAGCTTC GGTGTTGAAG 
GACTTTGGGA ACCTGGACGA GGGCGTGTTT CGGAGATATA CAAAACAAAT ACTGGAAGGA 
GTTTGCTACC TTCACAAGCA TAATGTGGTG CACAGAGACA TAAACGGGAA CAACGTGATG 
CTTCTACCCT GTGGAACTAT TAAGTTAATT GACTTCGGTT GCGCGAAAAA GATCCATGAG 
ATCAACAACA GTTCATCGGG GCGTAACAGT GCCTCTAGTG GTAGAGAGCG ACAGTTCAAG 
TCGGTGGTTG GGACCCCATA TTGGATGGCA CCCGAGGTCA TTAACGGGAA GGCTAAGTAC 
GGACCAAAGT CCGATGTTTG GAGCTTGGGA TGCACGGTGA TAGAGATGGC CACAGCGAAG 
CCCCCGCTGC ATGAGTTGGG AATATACGGG GCTATGTACC ACATCGGGGA AGGTAGACCG 
ATGCCTGTAT TATCGAACAA CTTCACTAAA CATGCGAGGT CCTTTGTTAT TCAATGCTTA 
AGAATCGATC CATCGTCACG GCCGACTGCA GACGAATTGT TACAACATAA GTTTATGCGA 
CATAGAAGGT TCGTAAGGAA GTCATTGGAA TAA