ORF » Species Summary » Chlamydomonas reinhardtii » ISA1 cDNA ORF clone

ISA1 cDNA ORF clone, Chlamydomonas reinhardtii

The following ISA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCh00061 XM_001697487.1
Latest version!		 Chlamydomonas reinhardtii isoamylase, starch debranching enzyme (ISA1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ISA1 ( XM_001697487.1 ) cDNA ORF clone, Chlamydomonas reinhardtii -> XP_001697539.1 Chlamydomonas reinhardtii isoamylase, starch debranching enzyme (ISA1), partial mRNA.
    Price & Availability↑
    CloneID OCh00061
    Clone ID Related Accession (Same CDS sequence) XM_001697487.1
    Accession Version XM_001697487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-08-28
    Organism Chlamydomonas reinhardtii
    Product isoamylase, starch debranching enzyme
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843754). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTGC AAGCCCCTGG CCTCGCGCCG GGCTCGGCTC GTCGCCAGGC AGCCTGCAGC 
    GTGGCTCGCG AGGCAACCAA CGTGCGCATT GTGACAGCAC CTGCTTTGAC ACCCGGGCGC 
    GCTGGAGTTT CTGGCCGGCG TATCCTCCCG CCCTCGCGCG TCGTGTCCGT GGAGCTGGAG 
    GCCCCGACGC TGTCGTCGAG CCCTGCGACT GTCAGCACCA AGAAGTTGTT CTGCGAGCCG 
    AGCGGGCAGC CCGCATCTAC TGCCTATGGG CCCGCCCTGA CCGGGCGCCC AGCTCCTTTG 
    GGCGCCAGCA TCGACGCTGA CACGGGCGCC ATCAACTTCT CAGTGTTCAG CTCCTCCGCC 
    GAGTCCGTGA GCCTGGTGCT GTTCACGGAG GCTGACCTCA ACGCAGGCCG GGCCACTTTC 
    GAGATTCCTC TGGACCCGTA TGTGAACCGC ACGGGCGACG TGTGGCACAT CATGCTGCCC 
    GACCTGCGGG ACGACCTGCT GTACGGCTAC CGTGTGGAGG GCGTGCACCA GGAGGAGGAC 
    AAGGACTACC CGGGCATGAG GCACGACAAG CGGCGTGTGG TTCTGGACCC GTACGCGGTG 
    GCTGTGCTCA ACCGGCGGCG CTGGGGCCAG ATGGGGCCCA ACCTGCCGTA CGGCGAGGAG 
    GGCGTGCTGG GCGTCATGCC CACGTGGCCG CAGGCCGCCG CCGCGCTGCC CGCCGCCCGC 
    GGCTCCGCCT TCGACTGGGA GGGCGACACG CCGCTCAACC TGCCCATGGA GAGCCTGGTC 
    ATCTACGAGG CGCACGTGAG GGGCTTCACG GCGCACGCCA GCAGCGGGGT GGCGGCGCCG 
    GGCACGTACG CGGGCATGGT GGAGCGGCTG GACTACCTCA AGTCGCTGGG TGTGAACGCC 
    ATTGAGCTGC TGCCCGTGTT CGAGTTCAAC GAGCTCGAGT ACTACAGCCA GATCCCCGGA 
    AGCGACCAGT ACAGGTTCAA CTTCTGGGGC TACTCCACGG TCAACTACTT CAGCCCCATG 
    GGCCGCTTCA GCGCTGCGGT GGGCCAGGGC GCGCCGGCCC GCGCCTCCTG CGACGAGTTC 
    AAGCAGCTGG TCAAGGAGTG CCACAGGCGC GGCATCGAGG GCGGCTGCGG CAACACGCTC 
    AACTGCAACC AGCCTGTGGT GCGGCAGTTC ATCCTGGATT GCCTCAAGCA CTGGGTCACC 
    GAGTACCACG TGGACGGGTT CAGGTTCGAC CTGGCCTCCA TCCTGACCCG CGCCCACTCG 
    GCCTGGCACC CGCAGCAGTA CGACCAGGAG ACGGGGCAGC GCGTGGCCAT GAGCAGCGGC 
    GGAGCCATCG TCACAGCGGA GGGCATCATG ACTGACGGTG CGGGTGTGCC CACCGGCTAC 
    CCGCTGGCCG ACCCGCCTCT GGTGGAGTCC ATCAGCGAGG ACCCCGTGCT GCGGAACACC 
    AAGATGATCG CGGAGGCCTG GGACTGCGAC GGACTCAACC AGGTCGGCGC CTTCCCGCAC 
    TACGGCGGCC GCTGGAGCGA GTGGAACGGC AAGTTCCGCG ACGTGGTTCG CAACTTCATC 
    AAGGGCACGG ACGGCCCCTG GGCGGGCGAC TTCGCCTCCG CCATCTGCGG CTCGCCCAAC 
    ATCTACGCCA ACAACACGCC GCACGAGACC GACTGGTGGG CCAACAACGG CGGGCGGCAG 
    TGGAAGGGCG GCCGCGGCCC GCACGCCTCC ATCAACTTCG TGGCGGCGCA TGACGGCTTC 
    ACGCTGGCAG ACATGGTGGC CTACAACAAC AAGCACAACG AGGCCAACGG AGAGAACAAC 
    CGGGACGGCG AGCAACACAA CAACAGCTGG AACTGCGGCG AGGAGGGGCC CACCACCAAG 
    TGGGAGGTCA ACCGGCTGCG TCAGCGCCAG ATGCGCAACC TGACCGGCGC GCTGCTGCTG 
    TCGTGCGGCG TGCCCATGAT CAACATGGGC GACGAGTACG GGCACAGCAA GAACGGCAAC 
    AACAACACCT ACTGCCACGA CAGCGAGCTA AACTACCTGC GGTGGGACCA GCTGGCCGAG 
    GACCCGCACG GCTTCAACCG CTTCGTGCGC CTGCTGATCC ACTTCCGCCG CGCCACGCCC 
    GCGCTGCAGC GCACCACCTT TGTCAACGAC AAGGACATCC AGTGGCACGG CGAGCTGCCC 
    AACACGCCCG ACTGGACCGA CACCAGCCGC CTGGTGGCCT TCACGCTGCA CGACGGCAAG 
    GGCGGCGGCC TGTACGTGGC CTTCAACACC AGCCACCTGC CCAAGCTGCT GCAGCTGCCC 
    AAGTGGGGCG GCCGCGTGTG GCAGCCGCTG GTGGACACCA GCAAGGTGGC CCCCTACGAC 
    TTCCTGGCGG TGGACGGCGT TCTGAGCGCC GAGGACGTGG CGGCGGCGCG GCGGCAGATG 
    GCCATGTGGA CCGCCGACCA CACCTACCCC GTGCTGCCCT GGAGCTGCAT CGTGCTGCAG 
    AGCGCGCCCG AGGACCCGGC CGCCACAAGC ATGATCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001697539.1
    CDS1..2502
    Translation

    Target ORF information:

    RefSeq Version XM_001697487.1
    Organism Chlamydomonas reinhardtii
    Definition Chlamydomonas reinhardtii isoamylase, starch debranching enzyme (ISA1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001697487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTTCTGC AAGCCCCTGG CCTCGCGCCG GGCTCGGCTC GTCGCCAGGC AGCCTGCAGC 
    GTGGCTCGCG AGGCAACCAA CGTGCGCATT GTGACAGCAC CTGCTTTGAC ACCCGGGCGC 
    GCTGGAGTTT CTGGCCGGCG TATCCTCCCG CCCTCGCGCG TCGTGTCCGT GGAGCTGGAG 
    GCCCCGACGC TGTCGTCGAG CCCTGCGACT GTCAGCACCA AGAAGTTGTT CTGCGAGCCG 
    AGCGGGCAGC CCGCATCTAC TGCCTATGGG CCCGCCCTGA CCGGGCGCCC AGCTCCTTTG 
    GGCGCCAGCA TCGACGCTGA CACGGGCGCC ATCAACTTCT CAGTGTTCAG CTCCTCCGCC 
    GAGTCCGTGA GCCTGGTGCT GTTCACGGAG GCTGACCTCA ACGCAGGCCG GGCCACTTTC 
    GAGATTCCTC TGGACCCGTA TGTGAACCGC ACGGGCGACG TGTGGCACAT CATGCTGCCC 
    GACCTGCGGG ACGACCTGCT GTACGGCTAC CGTGTGGAGG GCGTGCACCA GGAGGAGGAC 
    AAGGACTACC CGGGCATGAG GCACGACAAG CGGCGTGTGG TTCTGGACCC GTACGCGGTG 
    GCTGTGCTCA ACCGGCGGCG CTGGGGCCAG ATGGGGCCCA ACCTGCCGTA CGGCGAGGAG 
    GGCGTGCTGG GCGTCATGCC CACGTGGCCG CAGGCCGCCG CCGCGCTGCC CGCCGCCCGC 
    GGCTCCGCCT TCGACTGGGA GGGCGACACG CCGCTCAACC TGCCCATGGA GAGCCTGGTC 
    ATCTACGAGG CGCACGTGAG GGGCTTCACG GCGCACGCCA GCAGCGGGGT GGCGGCGCCG 
    GGCACGTACG CGGGCATGGT GGAGCGGCTG GACTACCTCA AGTCGCTGGG TGTGAACGCC 
    ATTGAGCTGC TGCCCGTGTT CGAGTTCAAC GAGCTCGAGT ACTACAGCCA GATCCCCGGA 
    AGCGACCAGT ACAGGTTCAA CTTCTGGGGC TACTCCACGG TCAACTACTT CAGCCCCATG 
    GGCCGCTTCA GCGCTGCGGT GGGCCAGGGC GCGCCGGCCC GCGCCTCCTG CGACGAGTTC 
    AAGCAGCTGG TCAAGGAGTG CCACAGGCGC GGCATCGAGG GCGGCTGCGG CAACACGCTC 
    AACTGCAACC AGCCTGTGGT GCGGCAGTTC ATCCTGGATT GCCTCAAGCA CTGGGTCACC 
    GAGTACCACG TGGACGGGTT CAGGTTCGAC CTGGCCTCCA TCCTGACCCG CGCCCACTCG 
    GCCTGGCACC CGCAGCAGTA CGACCAGGAG ACGGGGCAGC GCGTGGCCAT GAGCAGCGGC 
    GGAGCCATCG TCACAGCGGA GGGCATCATG ACTGACGGTG CGGGTGTGCC CACCGGCTAC 
    CCGCTGGCCG ACCCGCCTCT GGTGGAGTCC ATCAGCGAGG ACCCCGTGCT GCGGAACACC 
    AAGATGATCG CGGAGGCCTG GGACTGCGAC GGACTCAACC AGGTCGGCGC CTTCCCGCAC 
    TACGGCGGCC GCTGGAGCGA GTGGAACGGC AAGTTCCGCG ACGTGGTTCG CAACTTCATC 
    AAGGGCACGG ACGGCCCCTG GGCGGGCGAC TTCGCCTCCG CCATCTGCGG CTCGCCCAAC 
    ATCTACGCCA ACAACACGCC GCACGAGACC GACTGGTGGG CCAACAACGG CGGGCGGCAG 
    TGGAAGGGCG GCCGCGGCCC GCACGCCTCC ATCAACTTCG TGGCGGCGCA TGACGGCTTC 
    ACGCTGGCAG ACATGGTGGC CTACAACAAC AAGCACAACG AGGCCAACGG AGAGAACAAC 
    CGGGACGGCG AGCAACACAA CAACAGCTGG AACTGCGGCG AGGAGGGGCC CACCACCAAG 
    TGGGAGGTCA ACCGGCTGCG TCAGCGCCAG ATGCGCAACC TGACCGGCGC GCTGCTGCTG 
    TCGTGCGGCG TGCCCATGAT CAACATGGGC GACGAGTACG GGCACAGCAA GAACGGCAAC 
    AACAACACCT ACTGCCACGA CAGCGAGCTA AACTACCTGC GGTGGGACCA GCTGGCCGAG 
    GACCCGCACG GCTTCAACCG CTTCGTGCGC CTGCTGATCC ACTTCCGCCG CGCCACGCCC 
    GCGCTGCAGC GCACCACCTT TGTCAACGAC AAGGACATCC AGTGGCACGG CGAGCTGCCC 
    AACACGCCCG ACTGGACCGA CACCAGCCGC CTGGTGGCCT TCACGCTGCA CGACGGCAAG 
    GGCGGCGGCC TGTACGTGGC CTTCAACACC AGCCACCTGC CCAAGCTGCT GCAGCTGCCC 
    AAGTGGGGCG GCCGCGTGTG GCAGCCGCTG GTGGACACCA GCAAGGTGGC CCCCTACGAC 
    TTCCTGGCGG TGGACGGCGT TCTGAGCGCC GAGGACGTGG CGGCGGCGCG GCGGCAGATG 
    GCCATGTGGA CCGCCGACCA CACCTACCCC GTGCTGCCCT GGAGCTGCAT CGTGCTGCAG 
    AGCGCGCCCG AGGACCCGGC CGCCACAAGC ATGATCAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Chlamydomonas genome reveals the evolution of key animal and plant functions.
    Science (New York, N.Y.)318(5848)245-50(2007 Oct)
    Merchant SS,Prochnik SE,Vallon O,Harris EH,Karpowicz SJ,Witman GB,Terry A,Salamov A,Fritz-Laylin LK,Mar?chal-Drouard L,Marshall WF,Qu LH,Nelson DR,Sanderfoot AA,Spalding MH,Kapitonov VV,Ren Q,Ferris P,Lindquist E,Shapiro H,Lucas SM,Grimwood J,Schmutz J,Cardol P,Cerutti H,Chanfreau G,Chen CL,Cognat V,Croft MT,Dent R,Dutcher S,Fern?ndez E,Fukuzawa H,Gonz?lez-Ballester D,Gonz?lez-Halphen D,Hallmann A,Hanikenne M,Hippler M,Inwood W,Jabbari K,Kalanon M,Kuras R,Lefebvre PA,Lemaire SD,Lobanov AV,Lohr M,Manuell A,Meier I,Mets L,Mittag M,Mittelmeier T,Moroney JV,Moseley J,Napoli C,Nedelcu AM,Niyogi K,Novoselov SV,Paulsen IT,Pazour G,Purton S,Ral JP,Ria?o-Pach?n DM,Riekhof W,Rymarquis L,Schroda M,Stern D,Umen J,Willows R,Wilson N,Zimmer SL,Allmer J,Balk J,Bisova K,Chen CJ,Elias M,Gendler K,Hauser C,Lamb MR,Ledford H,Long JC,Minagawa J,Page MD,Pan J,Pootakham W,Roje S,Rose A,Stahlberg E,Terauchi AM,Yang P,Ball S,Bowler C,Dieckmann CL,Gladyshev VN,Green P,Jorgensen R,Mayfield S,Mueller-Roeber B,Rajamani S,Sayre RT,Brokstein P,Dubchak I,Goodstein D,Hornick L,Huang YW,Jhaveri J,Luo Y,Mart?nez D,Ngau WC,Otillar B,Poliakov A,Porter A,Szajkowski L,Werner G,Zhou K,Grigoriev IV,Rokhsar DS,Grossman AR


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