Hmbs cDNA ORF clone, Castor canadensis(American beaver)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hmbs
Entrez Gene ID	109693345
Full Name	hydroxymethylbilane synthase
Gene Type	protein-coding
Organism	Castor canadensis(American beaver)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020174606.1	XP_020030195.1	porphobilinogen deaminase isoform X2
XM_020174605.1	XP_020030194.1	porphobilinogen deaminase isoform X1

Pathways from BioSystems
Gene Ontology

The following Hmbs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hmbs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa291513	XM_020174605.1 Latest version!	Castor canadensis hydroxymethylbilane synthase (Hmbs), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCa291514	XM_020174606.1 Latest version!	Castor canadensis hydroxymethylbilane synthase (Hmbs), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hmbs ( XM_020174605.1 ) cDNA ORF clone, Castor canadensis(American beaver) -> XP_020030194.1 Castor canadensis hydroxymethylbilane synthase (Hmbs), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa291513
Clone ID Related Accession (Same CDS sequence)	XM_020174605.1
Accession Version	XM_020174605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Castor canadensis(American beaver)
Product	porphobilinogen deaminase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017871346.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Castor canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTGT CCAAGGTGCC CTGGCAAGAG GAACAGGAAG AAAACAACTC AAAGATGAGA 
GTGATTCGAG TGGGCACCCG TAAGAGCCAG CTGGCTCGTA TACAAACAGA CAGTGTGGTG 
GCAATGCTGA AAGCCTTCTA TCCAGGCGTG CAGTTTGAAA TCATTGCCAT GTCCACAACA 
GGGGACAAAA TTCTTGATAC TGCACTCTCC AAGATTGGAG AGAAGAGCCT GTTTACCAAG 
GAGCTGGAAT ATGCCCTGGG AAAAAATGAA GTGGACCTGG TTGTTCATTC CCTGAAGGAC 
CTGCCTACCA TGCTACCTCC TGACTTCACA ATTGGAGCCA TCTGCAAGCG GGAGAACCCC 
TGTGATGCTG TTGTCTTTCA CCCAAAATTT GTTGGGAAGA CCCTAGAAAC CTTGCCAGAG 
AGGAGTGTAG TAGGAACCAG CTCCCTGCGG AGAGCAGCCC AGCTGCAGAG AAAGTTCCCA 
CATCTGGAGT TCAAGAGTAT TCGGGGAAAT CTCAACACCC GGCTTCAGAA GCTGGACGAG 
CAGCAGAAGT TCAGTGCCAT CATCCTGGCT GTAGCAGGCC TGCAGCGCAT GGGCTGGCAG 
AATCGGGTGG GGCAGATTTT ACACCCAGAG GAATGCATGT ATGCTGTGGG CCAGGGGGCC 
CTGGGCGTGG AAGTCCGAGC CAAGGACCAG GACATCTTGG ATCTAGTGTG TGTGTTGCAT 
GACCCTGAGA CTCTGCTGCG CTGCATTGCT GAAAGGGCCT TCCTGAGGCA CCTGGAAGGA 
GGCTGCAGTG TACCAGTAGC AGTGCATACA GTAATGAAGG ACGGGCAACT GTACCTGACT 
GGGGGAGTCT GGAGTCTAGA TGGCTCAGAT AGCATGCAAG AGACCATGCA GACTACCATC 
CATGTCCCTG CCCAGCATGA AGATGGCCCA GAAGATGATC CACAGCTGGT AGGCATCACT 
GCCCGGAACG TTCCACGAGG AGCCCAGCTG GCTGCCGAGA GTCTGGGCAC CAGCCTGGCC 
ACCTTGTTGC TGAACAAAGG AGCCAAGAAC ATCCTGGATG TTGCTCGGCA GCTTAATGAT 
GTCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020030194.1
CDS	385..1473
Translation	MPLSKVPWQEEQEENNSKMRVIRVGTRKSQLARIQTDSVVAMLKAFYPGVQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEYALGKNEVDLVVHSLKDLPTMLPPDFTIGAICKRENPCDAVVFHPKFVGKTLETLPERSVVGTSSLRRAAQLQRKFPHLEFKSIRGNLNTRLQKLDEQQKFSAIILAVAGLQRMGWQNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVCVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTVMKDGQLYLTGGVWSLDGSDSMQETMQTTIHVPAQHEDGPEDDPQLVGITARNVPRGAQLAAESLGTSLATLLLNKGAKNILDVARQLNDVQ

Target ORF information:

RefSeq Version	XM_020174605.1
Organism	Castor canadensis(American beaver)
Definition	Castor canadensis hydroxymethylbilane synthase (Hmbs), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020174605.1

ORF Insert Sequence:

ATGCCTTTGT CCAAGGTGCC CTGGCAAGAG GAACAGGAAG AAAACAACTC AAAGATGAGA 
GTGATTCGAG TGGGCACCCG TAAGAGCCAG CTGGCTCGTA TACAAACAGA CAGTGTGGTG 
GCAATGCTGA AAGCCTTCTA TCCAGGCGTG CAGTTTGAAA TCATTGCCAT GTCCACAACA 
GGGGACAAAA TTCTTGATAC TGCACTCTCC AAGATTGGAG AGAAGAGCCT GTTTACCAAG 
GAGCTGGAAT ATGCCCTGGG AAAAAATGAA GTGGACCTGG TTGTTCATTC CCTGAAGGAC 
CTGCCTACCA TGCTACCTCC TGACTTCACA ATTGGAGCCA TCTGCAAGCG GGAGAACCCC 
TGTGATGCTG TTGTCTTTCA CCCAAAATTT GTTGGGAAGA CCCTAGAAAC CTTGCCAGAG 
AGGAGTGTAG TAGGAACCAG CTCCCTGCGG AGAGCAGCCC AGCTGCAGAG AAAGTTCCCA 
CATCTGGAGT TCAAGAGTAT TCGGGGAAAT CTCAACACCC GGCTTCAGAA GCTGGACGAG 
CAGCAGAAGT TCAGTGCCAT CATCCTGGCT GTAGCAGGCC TGCAGCGCAT GGGCTGGCAG 
AATCGGGTGG GGCAGATTTT ACACCCAGAG GAATGCATGT ATGCTGTGGG CCAGGGGGCC 
CTGGGCGTGG AAGTCCGAGC CAAGGACCAG GACATCTTGG ATCTAGTGTG TGTGTTGCAT 
GACCCTGAGA CTCTGCTGCG CTGCATTGCT GAAAGGGCCT TCCTGAGGCA CCTGGAAGGA 
GGCTGCAGTG TACCAGTAGC AGTGCATACA GTAATGAAGG ACGGGCAACT GTACCTGACT 
GGGGGAGTCT GGAGTCTAGA TGGCTCAGAT AGCATGCAAG AGACCATGCA GACTACCATC 
CATGTCCCTG CCCAGCATGA AGATGGCCCA GAAGATGATC CACAGCTGGT AGGCATCACT 
GCCCGGAACG TTCCACGAGG AGCCCAGCTG GCTGCCGAGA GTCTGGGCAC CAGCCTGGCC 
ACCTTGTTGC TGAACAAAGG AGCCAAGAAC ATCCTGGATG TTGCTCGGCA GCTTAATGAT 
GTCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmbs ( XM_020174606.1 ) cDNA ORF clone, Castor canadensis(American beaver) -> XP_020030195.1 Castor canadensis hydroxymethylbilane synthase (Hmbs), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa291514
Clone ID Related Accession (Same CDS sequence)	XM_020174606.1
Accession Version	XM_020174606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Castor canadensis(American beaver)
Product	porphobilinogen deaminase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017871346.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Castor canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGTA GCCGAGACCC GGCTGCAACG ACGGAAGAAA ACAACTCAAA GATGAGAGTG 
ATTCGAGTGG GCACCCGTAA GAGCCAGCTG GCTCGTATAC AAACAGACAG TGTGGTGGCA 
ATGCTGAAAG CCTTCTATCC AGGCGTGCAG TTTGAAATCA TTGCCATGTC CACAACAGGG 
GACAAAATTC TTGATACTGC ACTCTCCAAG ATTGGAGAGA AGAGCCTGTT TACCAAGGAG 
CTGGAATATG CCCTGGGAAA AAATGAAGTG GACCTGGTTG TTCATTCCCT GAAGGACCTG 
CCTACCATGC TACCTCCTGA CTTCACAATT GGAGCCATCT GCAAGCGGGA GAACCCCTGT 
GATGCTGTTG TCTTTCACCC AAAATTTGTT GGGAAGACCC TAGAAACCTT GCCAGAGAGG 
AGTGTAGTAG GAACCAGCTC CCTGCGGAGA GCAGCCCAGC TGCAGAGAAA GTTCCCACAT 
CTGGAGTTCA AGAGTATTCG GGGAAATCTC AACACCCGGC TTCAGAAGCT GGACGAGCAG 
CAGAAGTTCA GTGCCATCAT CCTGGCTGTA GCAGGCCTGC AGCGCATGGG CTGGCAGAAT 
CGGGTGGGGC AGATTTTACA CCCAGAGGAA TGCATGTATG CTGTGGGCCA GGGGGCCCTG 
GGCGTGGAAG TCCGAGCCAA GGACCAGGAC ATCTTGGATC TAGTGTGTGT GTTGCATGAC 
CCTGAGACTC TGCTGCGCTG CATTGCTGAA AGGGCCTTCC TGAGGCACCT GGAAGGAGGC 
TGCAGTGTAC CAGTAGCAGT GCATACAGTA ATGAAGGACG GGCAACTGTA CCTGACTGGG 
GGAGTCTGGA GTCTAGATGG CTCAGATAGC ATGCAAGAGA CCATGCAGAC TACCATCCAT 
GTCCCTGCCC AGCATGAAGA TGGCCCAGAA GATGATCCAC AGCTGGTAGG CATCACTGCC 
CGGAACGTTC CACGAGGAGC CCAGCTGGCT GCCGAGAGTC TGGGCACCAG CCTGGCCACC 
TTGTTGCTGA ACAAAGGAGC CAAGAACATC CTGGATGTTG CTCGGCAGCT TAATGATGTC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020030195.1
CDS	141..1226
Translation	MSGSRDPAATTEENNSKMRVIRVGTRKSQLARIQTDSVVAMLKAFYPGVQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEYALGKNEVDLVVHSLKDLPTMLPPDFTIGAICKRENPCDAVVFHPKFVGKTLETLPERSVVGTSSLRRAAQLQRKFPHLEFKSIRGNLNTRLQKLDEQQKFSAIILAVAGLQRMGWQNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVCVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTVMKDGQLYLTGGVWSLDGSDSMQETMQTTIHVPAQHEDGPEDDPQLVGITARNVPRGAQLAAESLGTSLATLLLNKGAKNILDVARQLNDVQ

Target ORF information:

RefSeq Version	XM_020174606.1
Organism	Castor canadensis(American beaver)
Definition	Castor canadensis hydroxymethylbilane synthase (Hmbs), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020174606.1

ORF Insert Sequence:

ATGTCCGGTA GCCGAGACCC GGCTGCAACG ACGGAAGAAA ACAACTCAAA GATGAGAGTG 
ATTCGAGTGG GCACCCGTAA GAGCCAGCTG GCTCGTATAC AAACAGACAG TGTGGTGGCA 
ATGCTGAAAG CCTTCTATCC AGGCGTGCAG TTTGAAATCA TTGCCATGTC CACAACAGGG 
GACAAAATTC TTGATACTGC ACTCTCCAAG ATTGGAGAGA AGAGCCTGTT TACCAAGGAG 
CTGGAATATG CCCTGGGAAA AAATGAAGTG GACCTGGTTG TTCATTCCCT GAAGGACCTG 
CCTACCATGC TACCTCCTGA CTTCACAATT GGAGCCATCT GCAAGCGGGA GAACCCCTGT 
GATGCTGTTG TCTTTCACCC AAAATTTGTT GGGAAGACCC TAGAAACCTT GCCAGAGAGG 
AGTGTAGTAG GAACCAGCTC CCTGCGGAGA GCAGCCCAGC TGCAGAGAAA GTTCCCACAT 
CTGGAGTTCA AGAGTATTCG GGGAAATCTC AACACCCGGC TTCAGAAGCT GGACGAGCAG 
CAGAAGTTCA GTGCCATCAT CCTGGCTGTA GCAGGCCTGC AGCGCATGGG CTGGCAGAAT 
CGGGTGGGGC AGATTTTACA CCCAGAGGAA TGCATGTATG CTGTGGGCCA GGGGGCCCTG 
GGCGTGGAAG TCCGAGCCAA GGACCAGGAC ATCTTGGATC TAGTGTGTGT GTTGCATGAC 
CCTGAGACTC TGCTGCGCTG CATTGCTGAA AGGGCCTTCC TGAGGCACCT GGAAGGAGGC 
TGCAGTGTAC CAGTAGCAGT GCATACAGTA ATGAAGGACG GGCAACTGTA CCTGACTGGG 
GGAGTCTGGA GTCTAGATGG CTCAGATAGC ATGCAAGAGA CCATGCAGAC TACCATCCAT 
GTCCCTGCCC AGCATGAAGA TGGCCCAGAA GATGATCCAC AGCTGGTAGG CATCACTGCC 
CGGAACGTTC CACGAGGAGC CCAGCTGGCT GCCGAGAGTC TGGGCACCAG CCTGGCCACC 
TTGTTGCTGA ACAAAGGAGC CAAGAACATC CTGGATGTTG CTCGGCAGCT TAATGATGTC 
CAGTAA