LOC109684653 cDNA ORF clone, Castor canadensis(American beaver)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109684653
Entrez Gene ID	109684653
Full Name	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1
Synonyms	AGAP1
Gene Type	protein-coding
Organism	Castor canadensis(American beaver)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020161128.1	XP_020016717.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X1
XM_020161129.1	XP_020016718.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC109684653 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109684653 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa278765	XM_020161128.1 Latest version!	Castor canadensis arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 (LOC109684653), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCa278766	XM_020161129.1 Latest version!	Castor canadensis arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 (LOC109684653), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109684653 ( XM_020161128.1 ) cDNA ORF clone, Castor canadensis(American beaver) -> XP_020016717.1 Castor canadensis arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 (LOC109684653), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa278765
Clone ID Related Accession (Same CDS sequence)	XM_020161128.1
Accession Version	XM_020161128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Castor canadensis(American beaver)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017870251.1) and transcript sequence (GFFV01001386.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Castor canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2359 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTACCTGC TAGACGGTAG CGGCGAGCCC GCGTCGCCGC CCGCGGACGC GATGCCGCGC 
GGGACGCCCC CCAGGAAGAC GGTTTACCGC ATCTCCGTGA CCATGGTCAG GAAGGAGCAG 
CTGACAGCGC CGGGCTCCAG AGGTGCCGAC CCACGCCCGG CTCGGCGGCC CCGGCCGGCA 
CGCTCACCGG ACGCACCCGG GCGGCTCGTG GAGGAGGCGG AGGAGACCGC GGACGATGAG 
GACCGCGAGG GCCGCCAGCC ATGTTCCTGG GACTTTCGCA CTTTCCGCAC TCGTAGCACC 
GGGCAGCTGG AGCTCGGGCG GCTCCGGCCG TGTACACGCA ACCCGGAGCC CACGGACCTG 
GCCGGCAGCC CCACAGCGGT GGAGGGACCC GGCTCGCCCG CTCCTGGCAG CCCGGGTGTG 
GAGGGGACGG GAGCCGCCCC CCTGCGGCGG AGCCTCAGTT TCAGGCACTG GAGCGGGCCT 
GAGGCGTCGC GGGACCCGGC CCTGGACGGC GGAAGGCACC ACAGCAGCTC CGGGAGCCTG 
GCCTGGGCGC CCAGCGACGA GGCCGAAGCC GACCCCGAGA CTGCTCTGGA GCCCCCGGCC 
GCCGCGCAGC CGGCCTCGGA GAAGCGCAAC ACCTTGGATG TAGGTGAGGT GCTCAGCAAG 
AACGATGCGC TCTCAGATCT GGAGCGCTGG GAGCGGAGCA AGAGCAAGAA CCGCACGCTA 
GACAACAGCG ACCTGCAGCG GCTAGAGCGC GCGCGTGCTG CGGCTGCGGG CTCCGGCGCG 
GGCCCCGGCG CGGGCCCCGA GCACCGGCTG CTGCGCTTCT TCAGCGGCAT CTTCGCGCGC 
AGGGACGGCG CGCCCGGCGG GCCCTCACCC CGGAGCGGCG GGTCGCGGTC GCGCGGCTAC 
TTCAGCAGCC TGCGGCGGGC CCCGGCCGAC GCGCACTCGT CCAGCGCCGA GAGCATCGAC 
GGGTCCCCGC GCAGAGATGC CTTTGTGAAC AGCCAGGAAT GGACGTTGAG TCGATCGGTA 
CCAGAGCTCA AAGTGGGAAT CGTGGGTAAC TTGGCCAGTG GAAAATCGGC CCTGGTGCAC 
CGATACCTGA CGGGCACCTA TGTCCAGGAG GAGTCTCCCG AAGGTGGCAG GTTCAAGAAG 
GAGATTGTCG TTGATGGACA GAGCTATCTG CTGCTGATTA GAGATGAAGG GGGCCCCCCA 
GAGGCACAGT TCGCCATGTG GGTGGATGCG GTCATATTTG TCTTCAGCTT GGAGGACGAG 
ATAAGCTTCC AGACTGTCTA CCACTACTAC AGTCGAATGG CCAACTATCG GAGCACCAGC 
GACCTCCCGC TGGTGCTGGT GGGGACCCAG GATGCCATAA GTTCCACGAA CCCGCGGGTC 
ATTGATGATG CCAGGGCGCG GAAGCTCTCC AACGACCTGA AGAGGTGCAC ATACTACGAG 
ACGTGTGCCA CATACGGGCT CAATGTGGAG CGGGTCTTCC AGGACGTTGC CCAGAAGATC 
GTTGCCACAA GGAAGAAGCA GCAGCTGTCC ATAGGCCCCT GCAAGTCCTT GCCCAACTCT 
CCCAGCCACT CATCTGTCTG CTCCGCACAG GTGTCTGCCG TGCACATAAG CCAGACGAGT 
AACGGAGGTG GCAGTTTAAG TGACTATTCC TCTTCCGTCC CATCGACCCC AAGCACCAGC 
CAGAAGGAAC TTCGGATTGA CGCTCCTCCC ACTGCCAACA CCCCAACGCC CGTCCGCAAG 
CAGTCCAAGC GCCGGTCCAA TCTGTTCACT TCTCGGAAAG GGAGCGACCC AGACAAAGAG 
AAGAAAGGCC TGGAGAGCCG CGCAGACAGC ATCGGGAGCG GCCGAGCCAT CCCAATTAAA 
CAGGGCATGC TGTTGAAGCG AAGTGGCAAG TCATTGAATA AAGAGTGGAA GAAGAAATAC 
GTCACCCTGT GCGACAATGG CGTGCTGACC TATCATCCCA GTCTACATGA TTACATGCAG 
AATGTTCATG GTAAGGAGAT TGATCTCCTG AGAACCACGG TGAAAGTCCC AGGGAAGAGG 
CCACCCCGTG CCACGTCAGC CTGCGCACCC ATCTCCAGCC CCAAAACCAA TGGCCTGTCC 
AAGGACTTGA GCAGTTTACA CATCTCGCCA AATTCAGGGA ATGTCACTAG TGCGTCTGGG 
TCTCAGATGG CAAGCGGCAT CAGCCTGGTC TCCTTCAACA GCCGACCCGA CGGCATGCAC 
CAGCGCTCCT ACTCAGTCTC CAGTGCGGAC CAGTGGAGTG AGGCTACGGT CATTGCAAAC 
TCGGCCATCA GCAGTGACAC AGGGCTGGGT GACTCCGTGT GCTCCAGCCC AAGCATCTCC 
AGCAGCACCA GCCCCAAGCT CGATCCGCCC CCCTCTCCCC ACGCCAACAG AAAGAAGCAC 
CGAAGGAAGA AAAGTACAAG TAACTTCAAA GCCGACGGGC TCTCAGGCAC TGCTGAAGCC 
AAACGCAAAG CTTGGAAACT AAACCGTGTT GGTAGCCTGC GAAATATATA CAGCAGCAGC 
AGCACCAACA CCGAAGAACA AGAAGAAAAC TTTGAGTTTA TCATTGTGTC CCTCACTGGT 
CAGACATGGC ACTTTGAAGC CACCACATAT GAAGAGCGAG ATGCATGGGT CCAAGCCATC 
GAAAGCCAGA TCCTAGCCAG CTTGCAGTCC TGCGAGAGCA GCAAGAATAA GTCCCGACTG 
ACCAGCCAGA GCGAGGCCAT GGCCCTGCAG TCTATCCGAA ACATCCGGGG GAACTCCCGC 
TGTGTGGACT GCGACACCCA GAATCCCAAC TGGGCCAGTT TGAATTTGGG AGCCCTCATG 
TGCATCGAGT GCTCAGGTAT CCACCGGAAT CTTGGCACTC ACCTTTCCCG AGTGCGATCT 
CTGGACCTGG ATGACTGGCC CATCGAGCTG ATCAAGGTGA TGTCATCCAT CGGGAATGAG 
CTGGCCAACA GTGTGTGGGA AGAGAGCAGC CAGGGGCGGA CGAAACCCTC CTTAGACTCC 
ACAAGGGAAG AAAAGGAGCG GTGGATCCGA GCCAAGTATG AACAGAAACT CTTCCTGGCC 
CCGCTGCCAT GTACCGAGCT GTCCCTGGGT CAGCACCTGC TGCGTGCCAC TGCCGATGAG 
GACCTGCGGA CTGTCATCCT GCTGCTGGCC CATGGCTCCC GGGATGAAGT GAACGAGACC 
TGTGGGGAGG GAGATGGCCG CACGGCACTG CACCTGGCCT GCCGCAAGGG CAATGTGGTT 
CTGGCACAGC TGCTGATCTG GTACGGGGTG GACGTCATGG CCCGAGACGC CCACGGGAAC 
ACAGCGCTGG CCTACGCCCG GCAGGCCTCC AGCCAGGAGT GCATCGACGT GCTGCTGCAG 
TACGGCTGCC CGGACGAGCG CTTCGTGCTC ATGGCCACCC CCAACCTGTC CAGGAGGAAC 
AACGGCCGGA ACAACAGCAG CGGCAGGGTG CCCAGCATCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020016717.1
CDS	223..3687
Translation	MYLLDGSGEPASPPADAMPRGTPPRKTVYRISVTMVRKEQLTAPGSRGADPRPARRPRPARSPDAPGRLVEEAEETADDEDREGRQPCSWDFRTFRTRSTGQLELGRLRPCTRNPEPTDLAGSPTAVEGPGSPAPGSPGVEGTGAAPLRRSLSFRHWSGPEASRDPALDGGRHHSSSGSLAWAPSDEAEADPETALEPPAAAQPASEKRNTLDVGEVLSKNDALSDLERWERSKSKNRTLDNSDLQRLERARAAAAGSGAGPGAGPEHRLLRFFSGIFARRDGAPGGPSPRSGGSRSRGYFSSLRRAPADAHSSSAESIDGSPRRDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRSTSDLPLVLVGTQDAISSTNPRVIDDARARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDAPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLSKDLSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQWSEATVIANSAISSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSGTAEAKRKAWKLNRVGSLRNIYSSSSTNTEEQEENFEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNIRGNSRCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPIELIKVMSSIGNELANSVWEESSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTELSLGQHLLRATADEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLAQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRRNNGRNNSSGRVPSII

Target ORF information:

RefSeq Version	XM_020161128.1
Organism	Castor canadensis(American beaver)
Definition	Castor canadensis arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 (LOC109684653), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020161128.1

ORF Insert Sequence:

ATGTACCTGC TAGACGGTAG CGGCGAGCCC GCGTCGCCGC CCGCGGACGC GATGCCGCGC 
GGGACGCCCC CCAGGAAGAC GGTTTACCGC ATCTCCGTGA CCATGGTCAG GAAGGAGCAG 
CTGACAGCGC CGGGCTCCAG AGGTGCCGAC CCACGCCCGG CTCGGCGGCC CCGGCCGGCA 
CGCTCACCGG ACGCACCCGG GCGGCTCGTG GAGGAGGCGG AGGAGACCGC GGACGATGAG 
GACCGCGAGG GCCGCCAGCC ATGTTCCTGG GACTTTCGCA CTTTCCGCAC TCGTAGCACC 
GGGCAGCTGG AGCTCGGGCG GCTCCGGCCG TGTACACGCA ACCCGGAGCC CACGGACCTG 
GCCGGCAGCC CCACAGCGGT GGAGGGACCC GGCTCGCCCG CTCCTGGCAG CCCGGGTGTG 
GAGGGGACGG GAGCCGCCCC CCTGCGGCGG AGCCTCAGTT TCAGGCACTG GAGCGGGCCT 
GAGGCGTCGC GGGACCCGGC CCTGGACGGC GGAAGGCACC ACAGCAGCTC CGGGAGCCTG 
GCCTGGGCGC CCAGCGACGA GGCCGAAGCC GACCCCGAGA CTGCTCTGGA GCCCCCGGCC 
GCCGCGCAGC CGGCCTCGGA GAAGCGCAAC ACCTTGGATG TAGGTGAGGT GCTCAGCAAG 
AACGATGCGC TCTCAGATCT GGAGCGCTGG GAGCGGAGCA AGAGCAAGAA CCGCACGCTA 
GACAACAGCG ACCTGCAGCG GCTAGAGCGC GCGCGTGCTG CGGCTGCGGG CTCCGGCGCG 
GGCCCCGGCG CGGGCCCCGA GCACCGGCTG CTGCGCTTCT TCAGCGGCAT CTTCGCGCGC 
AGGGACGGCG CGCCCGGCGG GCCCTCACCC CGGAGCGGCG GGTCGCGGTC GCGCGGCTAC 
TTCAGCAGCC TGCGGCGGGC CCCGGCCGAC GCGCACTCGT CCAGCGCCGA GAGCATCGAC 
GGGTCCCCGC GCAGAGATGC CTTTGTGAAC AGCCAGGAAT GGACGTTGAG TCGATCGGTA 
CCAGAGCTCA AAGTGGGAAT CGTGGGTAAC TTGGCCAGTG GAAAATCGGC CCTGGTGCAC 
CGATACCTGA CGGGCACCTA TGTCCAGGAG GAGTCTCCCG AAGGTGGCAG GTTCAAGAAG 
GAGATTGTCG TTGATGGACA GAGCTATCTG CTGCTGATTA GAGATGAAGG GGGCCCCCCA 
GAGGCACAGT TCGCCATGTG GGTGGATGCG GTCATATTTG TCTTCAGCTT GGAGGACGAG 
ATAAGCTTCC AGACTGTCTA CCACTACTAC AGTCGAATGG CCAACTATCG GAGCACCAGC 
GACCTCCCGC TGGTGCTGGT GGGGACCCAG GATGCCATAA GTTCCACGAA CCCGCGGGTC 
ATTGATGATG CCAGGGCGCG GAAGCTCTCC AACGACCTGA AGAGGTGCAC ATACTACGAG 
ACGTGTGCCA CATACGGGCT CAATGTGGAG CGGGTCTTCC AGGACGTTGC CCAGAAGATC 
GTTGCCACAA GGAAGAAGCA GCAGCTGTCC ATAGGCCCCT GCAAGTCCTT GCCCAACTCT 
CCCAGCCACT CATCTGTCTG CTCCGCACAG GTGTCTGCCG TGCACATAAG CCAGACGAGT 
AACGGAGGTG GCAGTTTAAG TGACTATTCC TCTTCCGTCC CATCGACCCC AAGCACCAGC 
CAGAAGGAAC TTCGGATTGA CGCTCCTCCC ACTGCCAACA CCCCAACGCC CGTCCGCAAG 
CAGTCCAAGC GCCGGTCCAA TCTGTTCACT TCTCGGAAAG GGAGCGACCC AGACAAAGAG 
AAGAAAGGCC TGGAGAGCCG CGCAGACAGC ATCGGGAGCG GCCGAGCCAT CCCAATTAAA 
CAGGGCATGC TGTTGAAGCG AAGTGGCAAG TCATTGAATA AAGAGTGGAA GAAGAAATAC 
GTCACCCTGT GCGACAATGG CGTGCTGACC TATCATCCCA GTCTACATGA TTACATGCAG 
AATGTTCATG GTAAGGAGAT TGATCTCCTG AGAACCACGG TGAAAGTCCC AGGGAAGAGG 
CCACCCCGTG CCACGTCAGC CTGCGCACCC ATCTCCAGCC CCAAAACCAA TGGCCTGTCC 
AAGGACTTGA GCAGTTTACA CATCTCGCCA AATTCAGGGA ATGTCACTAG TGCGTCTGGG 
TCTCAGATGG CAAGCGGCAT CAGCCTGGTC TCCTTCAACA GCCGACCCGA CGGCATGCAC 
CAGCGCTCCT ACTCAGTCTC CAGTGCGGAC CAGTGGAGTG AGGCTACGGT CATTGCAAAC 
TCGGCCATCA GCAGTGACAC AGGGCTGGGT GACTCCGTGT GCTCCAGCCC AAGCATCTCC 
AGCAGCACCA GCCCCAAGCT CGATCCGCCC CCCTCTCCCC ACGCCAACAG AAAGAAGCAC 
CGAAGGAAGA AAAGTACAAG TAACTTCAAA GCCGACGGGC TCTCAGGCAC TGCTGAAGCC 
AAACGCAAAG CTTGGAAACT AAACCGTGTT GGTAGCCTGC GAAATATATA CAGCAGCAGC 
AGCACCAACA CCGAAGAACA AGAAGAAAAC TTTGAGTTTA TCATTGTGTC CCTCACTGGT 
CAGACATGGC ACTTTGAAGC CACCACATAT GAAGAGCGAG ATGCATGGGT CCAAGCCATC 
GAAAGCCAGA TCCTAGCCAG CTTGCAGTCC TGCGAGAGCA GCAAGAATAA GTCCCGACTG 
ACCAGCCAGA GCGAGGCCAT GGCCCTGCAG TCTATCCGAA ACATCCGGGG GAACTCCCGC 
TGTGTGGACT GCGACACCCA GAATCCCAAC TGGGCCAGTT TGAATTTGGG AGCCCTCATG 
TGCATCGAGT GCTCAGGTAT CCACCGGAAT CTTGGCACTC ACCTTTCCCG AGTGCGATCT 
CTGGACCTGG ATGACTGGCC CATCGAGCTG ATCAAGGTGA TGTCATCCAT CGGGAATGAG 
CTGGCCAACA GTGTGTGGGA AGAGAGCAGC CAGGGGCGGA CGAAACCCTC CTTAGACTCC 
ACAAGGGAAG AAAAGGAGCG GTGGATCCGA GCCAAGTATG AACAGAAACT CTTCCTGGCC 
CCGCTGCCAT GTACCGAGCT GTCCCTGGGT CAGCACCTGC TGCGTGCCAC TGCCGATGAG 
GACCTGCGGA CTGTCATCCT GCTGCTGGCC CATGGCTCCC GGGATGAAGT GAACGAGACC 
TGTGGGGAGG GAGATGGCCG CACGGCACTG CACCTGGCCT GCCGCAAGGG CAATGTGGTT 
CTGGCACAGC TGCTGATCTG GTACGGGGTG GACGTCATGG CCCGAGACGC CCACGGGAAC 
ACAGCGCTGG CCTACGCCCG GCAGGCCTCC AGCCAGGAGT GCATCGACGT GCTGCTGCAG 
TACGGCTGCC CGGACGAGCG CTTCGTGCTC ATGGCCACCC CCAACCTGTC CAGGAGGAAC 
AACGGCCGGA ACAACAGCAG CGGCAGGGTG CCCAGCATCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109684653 ( XM_020161129.1 ) cDNA ORF clone, Castor canadensis(American beaver) -> XP_020016718.1 Castor canadensis arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 (LOC109684653), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa278766
Clone ID Related Accession (Same CDS sequence)	XM_020161129.1
Accession Version	XM_020161129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Castor canadensis(American beaver)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017870251.1) and transcript sequence (GFFV01001386.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Castor canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2359 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTACCTGC TAGACGGTAG CGGCGAGCCC GCGTCGCCGC CCGCGGACGC GATGCCGCGC 
GGGACGCCCC CCAGGAAGAC GGTTTACCGC ATCTCCGTGA CCATGGTCAG GAAGGAGCAG 
CTGACAGCGC CGGGCTCCAG AGGTGCCGAC CCACGCCCGG CTCGGCGGCC CCGGCCGGCA 
CGCTCACCGG ACGCACCCGG GCGGCTCGTG GAGGAGGCGG AGGAGACCGC GGACGATGAG 
GACCGCGAGG GCCGCCAGCC ATGTTCCTGG GACTTTCGCA CTTTCCGCAC TCGTAGCACC 
GGGCAGCTGG AGCTCGGGCG GCTCCGGCCG TGTACACGCA ACCCGGAGCC CACGGACCTG 
GCCGGCAGCC CCACAGCGGT GGAGGGACCC GGCTCGCCCG CTCCTGGCAG CCCGGGTGTG 
GAGGGGACGG GAGCCGCCCC CCTGCGGCGG AGCCTCAGTT TCAGGCACTG GAGCGGGCCT 
GAGGCGTCGC GGGACCCGGC CCTGGACGGC GGAAGGCACC ACAGCAGCTC CGGGAGCCTG 
GCCTGGGCGC CCAGCGACGA GGCCGAAGCC GACCCCGAGA CTGCTCTGGA GCCCCCGGCC 
GCCGCGCAGC CGGCCTCGGA GAAGCGCAAC ACCTTGGATG TAGGTGAGGT GCTCAGCAAG 
AACGATGCGC TCTCAGATCT GGAGCGCTGG GAGCGGAGCA AGAGCAAGAA CCGCACGCTA 
GACAACAGCG ACCTGCAGCG GCTAGAGCGC GCGCGTGCTG CGGCTGCGGG CTCCGGCGCG 
GGCCCCGGCG CGGGCCCCGA GCACCGGCTG CTGCGCTTCT TCAGCGGCAT CTTCGCGCGC 
AGGGACGGCG CGCCCGGCGG GCCCTCACCC CGGAGCGGCG GGTCGCGGTC GCGCGGCTAC 
TTCAGCAGCC TGCGGCGGGC CCCGGCCGAC GCGCACTCGT CCAGCGCCGA GAGCATCGAC 
GGGTCCCCGC GCAGAGATGC CTTTGTGAAC AGCCAGGAAT GGACGTTGAG TCGATCGGTA 
CCAGAGCTCA AAGTGGGAAT CGTGGGTAAC TTGGCCAGTG GAAAATCGGC CCTGGTGCAC 
CGATACCTGA CGGGCACCTA TGTCCAGGAG GAGTCTCCCG AAGGTGGCAG GTTCAAGAAG 
GAGATTGTCG TTGATGGACA GAGCTATCTG CTGCTGATTA GAGATGAAGG GGGCCCCCCA 
GAGGCACAGT TCGCCATGTG GGTGGATGCG GTCATATTTG TCTTCAGCTT GGAGGACGAG 
ATAAGCTTCC AGACTGTCTA CCACTACTAC AGTCGAATGG CCAACTATCG GAGCACCAGC 
GACCTCCCGC TGGTGCTGGT GGGGACCCAG GATGCCATAA GTTCCACGAA CCCGCGGGTC 
ATTGATGATG CCAGGGCGCG GAAGCTCTCC AACGACCTGA AGAGGTGCAC ATACTACGAG 
ACGTGTGCCA CATACGGGCT CAATGTGGAG CGGGTCTTCC AGGACGTTGC CCAGAAGATC 
GTTGCCACAA GGAAGAAGCA GCAGCTGTCC ATAGGCCCCT GCAAGTCCTT GCCCAACTCT 
CCCAGCCACT CATCTGTCTG CTCCGCACAG GTGTCTGCCG TGCACATAAG CCAGACGAGT 
AACGGAGGTG GCAGTTTAAG TGACTATTCC TCTTCCGTCC CATCGACCCC AAGCACCAGC 
CAGAAGGAAC TTCGGATTGA CGCTCCTCCC ACTGCCAACA CCCCAACGCC CGTCCGCAAG 
CAGTCCAAGC GCCGGTCCAA TCTGTTCACT TCTCGGAAAG GGAGCGACCC AGACAAAGAG 
AAGAAAGGCC TGGAGAGCCG CGCAGACAGC ATCGGGAGCG GCCGAGCCAT CCCAATTAAA 
CAGGGCATGC TGTTGAAGCG AAGTGGCAAG TCATTGAATA AAGAGTGGAA GAAGAAATAC 
GTCACCCTGT GCGACAATGG CGTGCTGACC TATCATCCCA GTCTACATGA TTACATGCAG 
AATGTTCATG GTAAGGAGAT TGATCTCCTG AGAACCACGG TGAAAGTCCC AGGGAAGAGG 
CCACCCCGTG CCACGTCAGC CTGCGCACCC ATCTCCAGCC CCAAAACCAA TGGCCTGTCC 
AAGGACTTGA GCAGTTTACA CATCTCGCCA AATTCAGGGA ATGTCACTAG TGCGTCTGGG 
TCTCAGATGG CAAGCGGCAT CAGCCTGGTC TCCTTCAACA GCCGACCCGA CGGCATGCAC 
CAGCGCTCCT ACTCAGTCTC CAGTGCGGAC CAGTGGAGTG AGGCTACGGT CATTGCAAAC 
TCGGCCATCA GCAGTGACAC AGGGCTGGGT GACTCCGTGT GCTCCAGCCC AAGCATCTCC 
AGCAGCACCA GCCCCAAGCT CGATCCGCCC CCCTCTCCCC ACGCCAACAG AAAGAAGCAC 
CGAAGGAAGA AAAGTACAAG TAACTTCAAA GCCGACGGGC TCTCAGGCAC TGCTGAAGAA 
CAAGAAGAAA ACTTTGAGTT TATCATTGTG TCCCTCACTG GTCAGACATG GCACTTTGAA 
GCCACCACAT ATGAAGAGCG AGATGCATGG GTCCAAGCCA TCGAAAGCCA GATCCTAGCC 
AGCTTGCAGT CCTGCGAGAG CAGCAAGAAT AAGTCCCGAC TGACCAGCCA GAGCGAGGCC 
ATGGCCCTGC AGTCTATCCG AAACATCCGG GGGAACTCCC GCTGTGTGGA CTGCGACACC 
CAGAATCCCA ACTGGGCCAG TTTGAATTTG GGAGCCCTCA TGTGCATCGA GTGCTCAGGT 
ATCCACCGGA ATCTTGGCAC TCACCTTTCC CGAGTGCGAT CTCTGGACCT GGATGACTGG 
CCCATCGAGC TGATCAAGGT GATGTCATCC ATCGGGAATG AGCTGGCCAA CAGTGTGTGG 
GAAGAGAGCA GCCAGGGGCG GACGAAACCC TCCTTAGACT CCACAAGGGA AGAAAAGGAG 
CGGTGGATCC GAGCCAAGTA TGAACAGAAA CTCTTCCTGG CCCCGCTGCC ATGTACCGAG 
CTGTCCCTGG GTCAGCACCT GCTGCGTGCC ACTGCCGATG AGGACCTGCG GACTGTCATC 
CTGCTGCTGG CCCATGGCTC CCGGGATGAA GTGAACGAGA CCTGTGGGGA GGGAGATGGC 
CGCACGGCAC TGCACCTGGC CTGCCGCAAG GGCAATGTGG TTCTGGCACA GCTGCTGATC 
TGGTACGGGG TGGACGTCAT GGCCCGAGAC GCCCACGGGA ACACAGCGCT GGCCTACGCC 
CGGCAGGCCT CCAGCCAGGA GTGCATCGAC GTGCTGCTGC AGTACGGCTG CCCGGACGAG 
CGCTTCGTGC TCATGGCCAC CCCCAACCTG TCCAGGAGGA ACAACGGCCG GAACAACAGC 
AGCGGCAGGG TGCCCAGCAT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020016718.1
CDS	223..3609
Translation	MYLLDGSGEPASPPADAMPRGTPPRKTVYRISVTMVRKEQLTAPGSRGADPRPARRPRPARSPDAPGRLVEEAEETADDEDREGRQPCSWDFRTFRTRSTGQLELGRLRPCTRNPEPTDLAGSPTAVEGPGSPAPGSPGVEGTGAAPLRRSLSFRHWSGPEASRDPALDGGRHHSSSGSLAWAPSDEAEADPETALEPPAAAQPASEKRNTLDVGEVLSKNDALSDLERWERSKSKNRTLDNSDLQRLERARAAAAGSGAGPGAGPEHRLLRFFSGIFARRDGAPGGPSPRSGGSRSRGYFSSLRRAPADAHSSSAESIDGSPRRDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRSTSDLPLVLVGTQDAISSTNPRVIDDARARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDAPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLSKDLSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQWSEATVIANSAISSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSGTAEEQEENFEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNIRGNSRCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPIELIKVMSSIGNELANSVWEESSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTELSLGQHLLRATADEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLAQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRRNNGRNNSSGRVPSII

Target ORF information:

RefSeq Version	XM_020161129.1
Organism	Castor canadensis(American beaver)
Definition	Castor canadensis arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 (LOC109684653), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020161129.1

ORF Insert Sequence:

ATGTACCTGC TAGACGGTAG CGGCGAGCCC GCGTCGCCGC CCGCGGACGC GATGCCGCGC 
GGGACGCCCC CCAGGAAGAC GGTTTACCGC ATCTCCGTGA CCATGGTCAG GAAGGAGCAG 
CTGACAGCGC CGGGCTCCAG AGGTGCCGAC CCACGCCCGG CTCGGCGGCC CCGGCCGGCA 
CGCTCACCGG ACGCACCCGG GCGGCTCGTG GAGGAGGCGG AGGAGACCGC GGACGATGAG 
GACCGCGAGG GCCGCCAGCC ATGTTCCTGG GACTTTCGCA CTTTCCGCAC TCGTAGCACC 
GGGCAGCTGG AGCTCGGGCG GCTCCGGCCG TGTACACGCA ACCCGGAGCC CACGGACCTG 
GCCGGCAGCC CCACAGCGGT GGAGGGACCC GGCTCGCCCG CTCCTGGCAG CCCGGGTGTG 
GAGGGGACGG GAGCCGCCCC CCTGCGGCGG AGCCTCAGTT TCAGGCACTG GAGCGGGCCT 
GAGGCGTCGC GGGACCCGGC CCTGGACGGC GGAAGGCACC ACAGCAGCTC CGGGAGCCTG 
GCCTGGGCGC CCAGCGACGA GGCCGAAGCC GACCCCGAGA CTGCTCTGGA GCCCCCGGCC 
GCCGCGCAGC CGGCCTCGGA GAAGCGCAAC ACCTTGGATG TAGGTGAGGT GCTCAGCAAG 
AACGATGCGC TCTCAGATCT GGAGCGCTGG GAGCGGAGCA AGAGCAAGAA CCGCACGCTA 
GACAACAGCG ACCTGCAGCG GCTAGAGCGC GCGCGTGCTG CGGCTGCGGG CTCCGGCGCG 
GGCCCCGGCG CGGGCCCCGA GCACCGGCTG CTGCGCTTCT TCAGCGGCAT CTTCGCGCGC 
AGGGACGGCG CGCCCGGCGG GCCCTCACCC CGGAGCGGCG GGTCGCGGTC GCGCGGCTAC 
TTCAGCAGCC TGCGGCGGGC CCCGGCCGAC GCGCACTCGT CCAGCGCCGA GAGCATCGAC 
GGGTCCCCGC GCAGAGATGC CTTTGTGAAC AGCCAGGAAT GGACGTTGAG TCGATCGGTA 
CCAGAGCTCA AAGTGGGAAT CGTGGGTAAC TTGGCCAGTG GAAAATCGGC CCTGGTGCAC 
CGATACCTGA CGGGCACCTA TGTCCAGGAG GAGTCTCCCG AAGGTGGCAG GTTCAAGAAG 
GAGATTGTCG TTGATGGACA GAGCTATCTG CTGCTGATTA GAGATGAAGG GGGCCCCCCA 
GAGGCACAGT TCGCCATGTG GGTGGATGCG GTCATATTTG TCTTCAGCTT GGAGGACGAG 
ATAAGCTTCC AGACTGTCTA CCACTACTAC AGTCGAATGG CCAACTATCG GAGCACCAGC 
GACCTCCCGC TGGTGCTGGT GGGGACCCAG GATGCCATAA GTTCCACGAA CCCGCGGGTC 
ATTGATGATG CCAGGGCGCG GAAGCTCTCC AACGACCTGA AGAGGTGCAC ATACTACGAG 
ACGTGTGCCA CATACGGGCT CAATGTGGAG CGGGTCTTCC AGGACGTTGC CCAGAAGATC 
GTTGCCACAA GGAAGAAGCA GCAGCTGTCC ATAGGCCCCT GCAAGTCCTT GCCCAACTCT 
CCCAGCCACT CATCTGTCTG CTCCGCACAG GTGTCTGCCG TGCACATAAG CCAGACGAGT 
AACGGAGGTG GCAGTTTAAG TGACTATTCC TCTTCCGTCC CATCGACCCC AAGCACCAGC 
CAGAAGGAAC TTCGGATTGA CGCTCCTCCC ACTGCCAACA CCCCAACGCC CGTCCGCAAG 
CAGTCCAAGC GCCGGTCCAA TCTGTTCACT TCTCGGAAAG GGAGCGACCC AGACAAAGAG 
AAGAAAGGCC TGGAGAGCCG CGCAGACAGC ATCGGGAGCG GCCGAGCCAT CCCAATTAAA 
CAGGGCATGC TGTTGAAGCG AAGTGGCAAG TCATTGAATA AAGAGTGGAA GAAGAAATAC 
GTCACCCTGT GCGACAATGG CGTGCTGACC TATCATCCCA GTCTACATGA TTACATGCAG 
AATGTTCATG GTAAGGAGAT TGATCTCCTG AGAACCACGG TGAAAGTCCC AGGGAAGAGG 
CCACCCCGTG CCACGTCAGC CTGCGCACCC ATCTCCAGCC CCAAAACCAA TGGCCTGTCC 
AAGGACTTGA GCAGTTTACA CATCTCGCCA AATTCAGGGA ATGTCACTAG TGCGTCTGGG 
TCTCAGATGG CAAGCGGCAT CAGCCTGGTC TCCTTCAACA GCCGACCCGA CGGCATGCAC 
CAGCGCTCCT ACTCAGTCTC CAGTGCGGAC CAGTGGAGTG AGGCTACGGT CATTGCAAAC 
TCGGCCATCA GCAGTGACAC AGGGCTGGGT GACTCCGTGT GCTCCAGCCC AAGCATCTCC 
AGCAGCACCA GCCCCAAGCT CGATCCGCCC CCCTCTCCCC ACGCCAACAG AAAGAAGCAC 
CGAAGGAAGA AAAGTACAAG TAACTTCAAA GCCGACGGGC TCTCAGGCAC TGCTGAAGAA 
CAAGAAGAAA ACTTTGAGTT TATCATTGTG TCCCTCACTG GTCAGACATG GCACTTTGAA 
GCCACCACAT ATGAAGAGCG AGATGCATGG GTCCAAGCCA TCGAAAGCCA GATCCTAGCC 
AGCTTGCAGT CCTGCGAGAG CAGCAAGAAT AAGTCCCGAC TGACCAGCCA GAGCGAGGCC 
ATGGCCCTGC AGTCTATCCG AAACATCCGG GGGAACTCCC GCTGTGTGGA CTGCGACACC 
CAGAATCCCA ACTGGGCCAG TTTGAATTTG GGAGCCCTCA TGTGCATCGA GTGCTCAGGT 
ATCCACCGGA ATCTTGGCAC TCACCTTTCC CGAGTGCGAT CTCTGGACCT GGATGACTGG 
CCCATCGAGC TGATCAAGGT GATGTCATCC ATCGGGAATG AGCTGGCCAA CAGTGTGTGG 
GAAGAGAGCA GCCAGGGGCG GACGAAACCC TCCTTAGACT CCACAAGGGA AGAAAAGGAG 
CGGTGGATCC GAGCCAAGTA TGAACAGAAA CTCTTCCTGG CCCCGCTGCC ATGTACCGAG 
CTGTCCCTGG GTCAGCACCT GCTGCGTGCC ACTGCCGATG AGGACCTGCG GACTGTCATC 
CTGCTGCTGG CCCATGGCTC CCGGGATGAA GTGAACGAGA CCTGTGGGGA GGGAGATGGC 
CGCACGGCAC TGCACCTGGC CTGCCGCAAG GGCAATGTGG TTCTGGCACA GCTGCTGATC 
TGGTACGGGG TGGACGTCAT GGCCCGAGAC GCCCACGGGA ACACAGCGCT GGCCTACGCC 
CGGCAGGCCT CCAGCCAGGA GTGCATCGAC GTGCTGCTGC AGTACGGCTG CCCGGACGAG 
CGCTTCGTGC TCATGGCCAC CCCCAACCTG TCCAGGAGGA ACAACGGCCG GAACAACAGC 
AGCGGCAGGG TGCCCAGCAT CATCTGA