HMBS cDNA ORF clone, Cariama cristata(Red-legged seriema)

Gene
Clones
gRNAs

Summary

Gene Symbol	HMBS
Entrez Gene ID	104165295
Full Name	hydroxymethylbilane synthase
Gene Type	protein-coding
Organism	Cariama cristata(Red-legged seriema)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009704879.1	XP_009703181.1	porphobilinogen deaminase

Pathways from BioSystems
Gene Ontology

The following HMBS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMBS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa460492	XM_009704879.1 Latest version!	Cariama cristata hydroxymethylbilane synthase (HMBS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMBS ( XM_009704879.1 ) cDNA ORF clone, Cariama cristata(Red-legged seriema) -> XP_009703181.1 Cariama cristata hydroxymethylbilane synthase (HMBS), mRNA.

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CloneID	OCa460492
Clone ID Related Accession (Same CDS sequence)	XM_009704879.1
Accession Version	XM_009704879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Cariama cristata(Red-legged seriema)
Product	porphobilinogen deaminase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009628572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cariama cristata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGCAAAGT CTTCTCTAGC TACTGTATGG ACGACGTACC CTTTGTTTAG GGCTTTGCTT 
TCTGCTTCGT TGCAGCTGGC CCGGATTCAG ACCGACAGCA TAGTCGAGAT GCTCCGTGAG 
TTATACCCTG ACCGCCGCTT TGAGATCGTT GCCATGTCAA CGACCGGAGA CAAGATCTTG 
GATACAGCAC TTTCCAAGAT CGGGGAGAAA AGCCTCTTCA CCAAAGAACT GGAAAATGCA 
CTTGAAAGAG ATGAAGTTGA CCTCGTGGTT CACTCCTTGA AGGACCTGCC AACTTCTCTT 
CCTCCTGGCT TTACCATCGG CGCTGTCTGC AAAAGGGAAA ACCCGCTTGA CGCTGTTGTC 
TTTCATCCCA AAAACTGTGG GAAAACGCTG AGCCTCCTTC CTGAAAAGNN NNNGATTGGA 
ACCAGTTCAC TTCGGAGAGC AGCGCAGCTC AAAAAGAAGT TCCCTCAGTT AGAATTCAGG 
GATATTAGAG GGAATTTAAA TACCCGCTTA AAGAAGCTAG ATGAGAAGGA AGACTTCAGC 
GCCATCATCC TGGCTGCTGC TGGACTGAAG AGAATGGGCT GGGAGAATCG CATTGGCCAG 
CTCCTAAGCC CTGAAGATTG TCTGTATGCT GTTGGACAGG GTGCCTTAGC AGTGGAAGTT 
CGTGCCAAAG ACCAAGAGAT GCTCAATATG GTGTCTGCCC TGCATGATGC AGACACCGTG 
TTATGCTGCA TTGCCGAGAG AGCCTTTATG AAACGTTTGG AGGGTGGATG TAGCGTCCCT 
GTCGCCGTCA ACACTGTGCT GAAAGATGGC CAGTTGTATT TGACAGGAGC AGTCTACAGT 
TTGGATGGAT CCGATAGCCT GAAGGAGACT ATGCAGACCA GTGTTAGTTA TCCCCAACAG 
AATGAAGATG GACCAAATGA TGATGTGCAG CATGTTGGCA TCACAGCCAG GCATGTCCCT 
GGTCAGGCCC AGGAAGCTGC AGAGAACCTC GGTGTTGAAC TAGCTAGTTT ACTTCTGAGC 
AAGGGAGCGA AGCATATCCT TAGCGTGGCA AGGCAGCTCA ACGATGCCCG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009703181.1
CDS	1..1074
Translation	MAKSSLATVWTTYPLFRALLSASLQLARIQTDSIVEMLRELYPDRRFEIVAMSTTGDKILDTALSKIGEKSLFTKELENALERDEVDLVVHSLKDLPTSLPPGFTIGAVCKRENPLDAVVFHPKNCGKTLSLLPEKnullnullIGTSSLRRAAQLKKKFPQLEFRDIRGNLNTRLKKLDEKEDFSAIILAAAGLKRMGWENRIGQLLSPEDCLYAVGQGALAVEVRAKDQEMLNMVSALHDADTVLCCIAERAFMKRLEGGCSVPVAVNTVLKDGQLYLTGAVYSLDGSDSLKETMQTSVSYPQQNEDGPNDDVQHVGITARHVPGQAQEAAENLGVELASLLLSKGAKHILSVARQLNDAR

Target ORF information:

RefSeq Version	XM_009704879.1
Organism	Cariama cristata(Red-legged seriema)
Definition	Cariama cristata hydroxymethylbilane synthase (HMBS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009704879.1

ORF Insert Sequence:

ATGGCAAAGT CTTCTCTAGC TACTGTATGG ACGACGTACC CTTTGTTTAG GGCTTTGCTT 
TCTGCTTCGT TGCAGCTGGC CCGGATTCAG ACCGACAGCA TAGTCGAGAT GCTCCGTGAG 
TTATACCCTG ACCGCCGCTT TGAGATCGTT GCCATGTCAA CGACCGGAGA CAAGATCTTG 
GATACAGCAC TTTCCAAGAT CGGGGAGAAA AGCCTCTTCA CCAAAGAACT GGAAAATGCA 
CTTGAAAGAG ATGAAGTTGA CCTCGTGGTT CACTCCTTGA AGGACCTGCC AACTTCTCTT 
CCTCCTGGCT TTACCATCGG CGCTGTCTGC AAAAGGGAAA ACCCGCTTGA CGCTGTTGTC 
TTTCATCCCA AAAACTGTGG GAAAACGCTG AGCCTCCTTC CTGAAAAGNN NNNGATTGGA 
ACCAGTTCAC TTCGGAGAGC AGCGCAGCTC AAAAAGAAGT TCCCTCAGTT AGAATTCAGG 
GATATTAGAG GGAATTTAAA TACCCGCTTA AAGAAGCTAG ATGAGAAGGA AGACTTCAGC 
GCCATCATCC TGGCTGCTGC TGGACTGAAG AGAATGGGCT GGGAGAATCG CATTGGCCAG 
CTCCTAAGCC CTGAAGATTG TCTGTATGCT GTTGGACAGG GTGCCTTAGC AGTGGAAGTT 
CGTGCCAAAG ACCAAGAGAT GCTCAATATG GTGTCTGCCC TGCATGATGC AGACACCGTG 
TTATGCTGCA TTGCCGAGAG AGCCTTTATG AAACGTTTGG AGGGTGGATG TAGCGTCCCT 
GTCGCCGTCA ACACTGTGCT GAAAGATGGC CAGTTGTATT TGACAGGAGC AGTCTACAGT 
TTGGATGGAT CCGATAGCCT GAAGGAGACT ATGCAGACCA GTGTTAGTTA TCCCCAACAG 
AATGAAGATG GACCAAATGA TGATGTGCAG CATGTTGGCA TCACAGCCAG GCATGTCCCT 
GGTCAGGCCC AGGAAGCTGC AGAGAACCTC GGTGTTGAAC TAGCTAGTTT ACTTCTGAGC 
AAGGGAGCGA AGCATATCCT TAGCGTGGCA AGGCAGCTCA ACGATGCCCG CTAA