OTOF cDNA ORF clone, Canis lupus familiaris(dog)

The following OTOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OTOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe294274 XM_022404549.1
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe18751 XM_022404546.1
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe18751 XM_844665.4
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe18752 XM_005630229.2
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe18753 XM_005630230.2
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe18754 XM_005630231.2
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe18755 XM_022404547.1
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe18755 XM_005630232.2
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe18756 XM_005630233.2
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe18756 XM_005630234.2
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe18756 XM_022404548.1
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe294268 XM_005630234.3
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294269 XM_022404540.1
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294270 XM_022404541.1
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294271 XM_022404542.1
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294272 XM_022404543.1
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294273 XM_022404544.1
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe40800 XM_014120469.1
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe18750 XM_022404545.1
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe18750 XM_005630228.2
Latest version!
Canis lupus familiaris otoferlin (OTOF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe294274
    Clone ID Related Accession (Same CDS sequence) XM_022404549.1
    Accession Version XM_022404549.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATCAAGA CTGAGAAGTC TTACCCTGAG CGTCGCCTCC GGGGTGTCCT GGAGGAGCTC 
    AGCTGTGGCT GCTACCGCTT CCTCTCCCTC GCCGACAAGG ACCAGGGCCA CTCATCCCGC
    ACCAGGCTGG ACCGGGAGCG CCTCAAGTCC TGCATGCGGG AGCTGGAAAG CATGGGACAG
    CAGGCCAAGA CCCTGCGGGC TCAGGTGAAG AAGCACACGG TGCGGGACAA GCTGAGGCTA
    TGCCAGAATT TCCTGCAGAA GCTGCGCTTC CTGGCAGATG AGCCCCAGCA CAGCATCCCC
    GATGTCTTCA TCTGGATGAT GAGCAATAAC AAGCGCATTG CCTACGCCCG GGTGCCCTCC
    AAGGACCTGC TCTTCTCCAT CGTTGAGGAG GAGCTGGGAA AGGACTGCGC CAAGGTCAAG
    ACGCTCTTCC TCAAGCTGCC AGGCAAGCGG GGCTTCGGCT CAGCAGGCTG GACGGTGCAG
    GCCAAGCTGG AGCTCTACCT GTGGCTGGGC CTCAGCAAGC AGCGCAAGGA CTTCCTGTGC
    GGGCTGCCCT GTGGCTTCGA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCACTCCTTC
    CCACCCATCA GCCTGGTCTA CACCAAGAAG CAGGCCTTCC AGCTCCGAGC CCACATGTAC
    CAGGCTCGCA GCCTCTTTGC TGCTGACAGC AGTGGCCTCT CAGACCCCTT CGCCCGCGTC
    TTCTTCATCA ACCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG CCCCACCTGG
    GACCAGATGC TGGTATTTGA CAACCTGGAG CTTTATGGTG AAGCCCATGA GTTGAGGGAT
    GATCCCCCTA TCATTGTCAT CGAGATCTAT GACCAGGACG CCATGGGCAA AGCCGACTTC
    ATGGGCCGGA CCTTTGCCAA GCCCCTGGTG AAGATGGCCG ACGAGGCGTA CTGCCCACCC
    CGCTTCCCAC CCCAGCTCGA GTACTACCAA ATCTACAGAG GCAATGCCAC GGCTGGGGAT
    CTGCTGGCTG CCTTCGAGCT GCTGCAGATT GGGCCAGCAG GGAAGGCAGA CCTGCCACCC
    ATCAACGGCC CTGTGGACAT GGACCGAGGT CCCATCATGC CCGTGCCTGT GGGCATCCGG
    CCCGTGCTCA GCAAATACCG AATCGAGGTG CTCTTCTGGG GCCTGAGAGA CCTGAAGCGG
    GTGAACCTGG CCCAGGTGGA CCGGCCGCGG GTGGACATCG AGTGTGCAGG AAAGGGAGTG
    CAGTCGTCCC TGATCCACAA TTACAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG
    TTTGAAGTGG ACCTCCCAGA AAACGAGCTG TTGCACCCGC CCCTGAACAT CCGAGTGGTG
    GACTGCCGGG CCTTCGGCCG CTACACCCTG GTGGGCTCCC ATGCCGTCAG CGCTCTGCGG
    CGATTCATCT ACAGGCCCCC AGACCGCTCA GCCCCCAACT GGAACACCAC AGTCAGGCTC
    CTCCGGGGTG GCCGTGTGCT GTGCAATGGG GGCCCCTCCT CTCACCCCAC AGGGGAAGTC
    GTGGTGAACA TGGAGCCAGA GGTGCCTGTC AAGAAGCTGG AGACCATGGT GAAGCTGGAC
    GCGACTTCTG ATGCTGTCAT CAAGGTGGAT GTGGCTGACG AGGAGAAGGA GAAGAAAAAG
    AAGAAGAAGG GCAACGCAGA AGAGCCAGAG GAAGAGGAGC CAGATGAGAG CATGCTGGAC
    TGGTGGTCCA AGTACTTTGC TTCCATCGAT ACCATGAAGG AGCAACTTCG GCAGCAAGAG
    GTCTCAGGCA CTGACTTGGA GGAGAAAGAG GAGGTGGACA ACACCGAGGG CCTGAAGGGA
    CCGATGAAGG TCAAGGAGAA GGCAAAGGCA GCAAAGGAAG AGAAGAAAAA GAGAACCCAG
    GGCCCCGGCG CCGGCCAGGG GTCTGAAGTC CCTGAGAAGA AGAAACCCAA GATTGATGAG
    CTGAAGGTGT ACTCCAAGGA GCTGGAGTCC GAGTTCGACA ACTTTGAGGA CTGGCTGCAC
    ACTTTCAACC TGCTGCGGGG CAAGACGGGA GATGATGAGG ACGGCTCCAC TGAGGAGGAG
    CGCATCGTGG GCAGGTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAT
    GTGTCCCGGG AAGCCGGCTA CGACCCCACC TATGGCATGT TCCAGGGCAT TCCCAGTAAT
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCAGGG CCACGGACCT GCACCCCGCA
    GACATCAACG GCAAAGCAGA CCCCTACATC GCCATCCGGC TGGGCAAGAC AGACATCCGG
    GACAAGGAGA ACTACATCTC TAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATT
    GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCCG TGTACGACTG GGACCTGGTC
    GGCACCGACG ACCTCATTGG GGAAACCAAG ATTGACCTGG AAAACCGCTT CTACAGCAAG
    CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCATAC ATGGCTACAA TATCTGGCGG
    GACCCCATGA AGCCCAGCCA GATTCTGACC CGTCTCTGCA AGGAGGGCAA AGTGGATGGC
    CCCCACTTTG GGCCCCCCGG GAGAGTGAAA GTGTCCAACC GCGTCTTCAC TGGGCCCTCC
    GAGATCGAGG ACGAGAATGG TCAGAGGAAG CCCACAGAAG AGCACGTGGC ACTGTCTGCC
    CTAAGGCACT GGGAGGACAT CCCCCGTGTG GGCTGCCGCC TCGTGCCTGA ACATGTGGAG
    ACTCGGCCGC TGCTCAACCC TGACAAGCCA GGCATCGAGC AGGGCCGCCT GGAGCTGTGG
    GTGGATATGT TCCCCATGGA CATGCCAGCA CCCGGCACAC CACTGGACAT CTCCCCTCGG
    AAGCCCAAGA AGTACGAGCT CCGGGTCATC GTGTGGAACA CGGACGAGGT GGTCCTGGAG
    GACGATGACT TCTTTACTGG GGAGAAGTCC AGCGACATTT TTGTGCGGGG GTGGCTGAAG
    GGCCAGCAGG AGGACAAGCA GGACACGGAT GTCCACTATC ACTCCCTCAC CGGCGAGGGC
    AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTATCTGG CCGCCGAGGA AAAGATTGTC
    ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACGG AGTACAAGAT CCCCGCGCGG
    CTCACCTTGC AGATCTGGGA CGCCGACCAC TTCTCCGCGG ACGATTTCCT GGGGGCCATC
    GAGCTGGACC TGAACCGGTT CCCCCGGGGC GCGAAGACAG CCAAGCAGTG CACCATGGAG
    ATGGCCACGG GGGAGGTGGA CGTGCCCCTG GTTTCCATCT TCAAGCAGAA GCGCGTCAAA
    GGCTGGTGGC CGCTCCTGGC CCGCGATGAG AATGATGAGT TCGAGCTCAC GGGCAAGGTG
    GAGGCAGAGT TGCATCTCCT GACAGCCGAG GAGGCAGAGA AGAACCCCGT GGGCCTGGCC
    CGCAATGAAC CTGACCCCCT AGAGAAACCC AAGCGGCCAG ACACGAGCTT CATCTGGTTC
    TTGAACCCTC TCAAGTCTGC CCGCTACTTC TTGTGGCACA CATACCGCTG GCTGCTCCTC
    AAACTGCTGC TGCTCATGCT GCTGCTCCTC CTCCTCGCCC TCTTCCTCTA CTCTGTGCCC
    GGCTACCTGG TCAAGAAGAT CCTGGGGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260257.1
    CDS527..4279
    Translation

    Target ORF information:

    RefSeq Version XM_022404549.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404549.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATCAAGA CTGAGAAGTC TTACCCTGAG CGTCGCCTCC GGGGTGTCCT GGAGGAGCTC 
    AGCTGTGGCT GCTACCGCTT CCTCTCCCTC GCCGACAAGG ACCAGGGCCA CTCATCCCGC
    ACCAGGCTGG ACCGGGAGCG CCTCAAGTCC TGCATGCGGG AGCTGGAAAG CATGGGACAG
    CAGGCCAAGA CCCTGCGGGC TCAGGTGAAG AAGCACACGG TGCGGGACAA GCTGAGGCTA
    TGCCAGAATT TCCTGCAGAA GCTGCGCTTC CTGGCAGATG AGCCCCAGCA CAGCATCCCC
    GATGTCTTCA TCTGGATGAT GAGCAATAAC AAGCGCATTG CCTACGCCCG GGTGCCCTCC
    AAGGACCTGC TCTTCTCCAT CGTTGAGGAG GAGCTGGGAA AGGACTGCGC CAAGGTCAAG
    ACGCTCTTCC TCAAGCTGCC AGGCAAGCGG GGCTTCGGCT CAGCAGGCTG GACGGTGCAG
    GCCAAGCTGG AGCTCTACCT GTGGCTGGGC CTCAGCAAGC AGCGCAAGGA CTTCCTGTGC
    GGGCTGCCCT GTGGCTTCGA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCACTCCTTC
    CCACCCATCA GCCTGGTCTA CACCAAGAAG CAGGCCTTCC AGCTCCGAGC CCACATGTAC
    CAGGCTCGCA GCCTCTTTGC TGCTGACAGC AGTGGCCTCT CAGACCCCTT CGCCCGCGTC
    TTCTTCATCA ACCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG CCCCACCTGG
    GACCAGATGC TGGTATTTGA CAACCTGGAG CTTTATGGTG AAGCCCATGA GTTGAGGGAT
    GATCCCCCTA TCATTGTCAT CGAGATCTAT GACCAGGACG CCATGGGCAA AGCCGACTTC
    ATGGGCCGGA CCTTTGCCAA GCCCCTGGTG AAGATGGCCG ACGAGGCGTA CTGCCCACCC
    CGCTTCCCAC CCCAGCTCGA GTACTACCAA ATCTACAGAG GCAATGCCAC GGCTGGGGAT
    CTGCTGGCTG CCTTCGAGCT GCTGCAGATT GGGCCAGCAG GGAAGGCAGA CCTGCCACCC
    ATCAACGGCC CTGTGGACAT GGACCGAGGT CCCATCATGC CCGTGCCTGT GGGCATCCGG
    CCCGTGCTCA GCAAATACCG AATCGAGGTG CTCTTCTGGG GCCTGAGAGA CCTGAAGCGG
    GTGAACCTGG CCCAGGTGGA CCGGCCGCGG GTGGACATCG AGTGTGCAGG AAAGGGAGTG
    CAGTCGTCCC TGATCCACAA TTACAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG
    TTTGAAGTGG ACCTCCCAGA AAACGAGCTG TTGCACCCGC CCCTGAACAT CCGAGTGGTG
    GACTGCCGGG CCTTCGGCCG CTACACCCTG GTGGGCTCCC ATGCCGTCAG CGCTCTGCGG
    CGATTCATCT ACAGGCCCCC AGACCGCTCA GCCCCCAACT GGAACACCAC AGTCAGGCTC
    CTCCGGGGTG GCCGTGTGCT GTGCAATGGG GGCCCCTCCT CTCACCCCAC AGGGGAAGTC
    GTGGTGAACA TGGAGCCAGA GGTGCCTGTC AAGAAGCTGG AGACCATGGT GAAGCTGGAC
    GCGACTTCTG ATGCTGTCAT CAAGGTGGAT GTGGCTGACG AGGAGAAGGA GAAGAAAAAG
    AAGAAGAAGG GCAACGCAGA AGAGCCAGAG GAAGAGGAGC CAGATGAGAG CATGCTGGAC
    TGGTGGTCCA AGTACTTTGC TTCCATCGAT ACCATGAAGG AGCAACTTCG GCAGCAAGAG
    GTCTCAGGCA CTGACTTGGA GGAGAAAGAG GAGGTGGACA ACACCGAGGG CCTGAAGGGA
    CCGATGAAGG TCAAGGAGAA GGCAAAGGCA GCAAAGGAAG AGAAGAAAAA GAGAACCCAG
    GGCCCCGGCG CCGGCCAGGG GTCTGAAGTC CCTGAGAAGA AGAAACCCAA GATTGATGAG
    CTGAAGGTGT ACTCCAAGGA GCTGGAGTCC GAGTTCGACA ACTTTGAGGA CTGGCTGCAC
    ACTTTCAACC TGCTGCGGGG CAAGACGGGA GATGATGAGG ACGGCTCCAC TGAGGAGGAG
    CGCATCGTGG GCAGGTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAT
    GTGTCCCGGG AAGCCGGCTA CGACCCCACC TATGGCATGT TCCAGGGCAT TCCCAGTAAT
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCAGGG CCACGGACCT GCACCCCGCA
    GACATCAACG GCAAAGCAGA CCCCTACATC GCCATCCGGC TGGGCAAGAC AGACATCCGG
    GACAAGGAGA ACTACATCTC TAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATT
    GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCCG TGTACGACTG GGACCTGGTC
    GGCACCGACG ACCTCATTGG GGAAACCAAG ATTGACCTGG AAAACCGCTT CTACAGCAAG
    CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCATAC ATGGCTACAA TATCTGGCGG
    GACCCCATGA AGCCCAGCCA GATTCTGACC CGTCTCTGCA AGGAGGGCAA AGTGGATGGC
    CCCCACTTTG GGCCCCCCGG GAGAGTGAAA GTGTCCAACC GCGTCTTCAC TGGGCCCTCC
    GAGATCGAGG ACGAGAATGG TCAGAGGAAG CCCACAGAAG AGCACGTGGC ACTGTCTGCC
    CTAAGGCACT GGGAGGACAT CCCCCGTGTG GGCTGCCGCC TCGTGCCTGA ACATGTGGAG
    ACTCGGCCGC TGCTCAACCC TGACAAGCCA GGCATCGAGC AGGGCCGCCT GGAGCTGTGG
    GTGGATATGT TCCCCATGGA CATGCCAGCA CCCGGCACAC CACTGGACAT CTCCCCTCGG
    AAGCCCAAGA AGTACGAGCT CCGGGTCATC GTGTGGAACA CGGACGAGGT GGTCCTGGAG
    GACGATGACT TCTTTACTGG GGAGAAGTCC AGCGACATTT TTGTGCGGGG GTGGCTGAAG
    GGCCAGCAGG AGGACAAGCA GGACACGGAT GTCCACTATC ACTCCCTCAC CGGCGAGGGC
    AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTATCTGG CCGCCGAGGA AAAGATTGTC
    ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACGG AGTACAAGAT CCCCGCGCGG
    CTCACCTTGC AGATCTGGGA CGCCGACCAC TTCTCCGCGG ACGATTTCCT GGGGGCCATC
    GAGCTGGACC TGAACCGGTT CCCCCGGGGC GCGAAGACAG CCAAGCAGTG CACCATGGAG
    ATGGCCACGG GGGAGGTGGA CGTGCCCCTG GTTTCCATCT TCAAGCAGAA GCGCGTCAAA
    GGCTGGTGGC CGCTCCTGGC CCGCGATGAG AATGATGAGT TCGAGCTCAC GGGCAAGGTG
    GAGGCAGAGT TGCATCTCCT GACAGCCGAG GAGGCAGAGA AGAACCCCGT GGGCCTGGCC
    CGCAATGAAC CTGACCCCCT AGAGAAACCC AAGCGGCCAG ACACGAGCTT CATCTGGTTC
    TTGAACCCTC TCAAGTCTGC CCGCTACTTC TTGTGGCACA CATACCGCTG GCTGCTCCTC
    AAACTGCTGC TGCTCATGCT GCTGCTCCTC CTCCTCGCCC TCTTCCTCTA CTCTGTGCCC
    GGCTACCTGG TCAAGAAGAT CCTGGGGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18751
    Clone ID Related Accession (Same CDS sequence) XM_022404546.1 , XM_844665.4
    Accession Version XM_022404546.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGTCAGGCT CCTCCGGGGT GGCCGTGTGC TGTGCAATGG GGGCCCCTCC
    TCTCACCCCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAAGCGGCCA
    GACACGAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CTTGTGGCAC
    ACATACCGCT GGCTGCTCCT CAAACTGCTG CTGCTCATGC TGCTGCTCCT CCTCCTCGCC
    CTCTTCCTCT ACTCTGTGCC CGGCTACCTG GTCAAGAAGA TCCTGGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260254.1
    CDS1..5994
    Translation

    Target ORF information:

    RefSeq Version XM_022404546.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGTCAGGCT CCTCCGGGGT GGCCGTGTGC TGTGCAATGG GGGCCCCTCC
    TCTCACCCCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAAGCGGCCA
    GACACGAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CTTGTGGCAC
    ACATACCGCT GGCTGCTCCT CAAACTGCTG CTGCTCATGC TGCTGCTCCT CCTCCTCGCC
    CTCTTCCTCT ACTCTGTGCC CGGCTACCTG GTCAAGAAGA TCCTGGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18751
    Clone ID Related Accession (Same CDS sequence) XM_022404546.1 , XM_844665.4
    Accession Version XM_844665.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product otoferlin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_844665.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGTCAGGCT CCTCCGGGGT GGCCGTGTGC TGTGCAATGG GGGCCCCTCC
    TCTCACCCCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAAGCGGCCA
    GACACGAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CTTGTGGCAC
    ACATACCGCT GGCTGCTCCT CAAACTGCTG CTGCTCATGC TGCTGCTCCT CCTCCTCGCC
    CTCTTCCTCT ACTCTGTGCC CGGCTACCTG GTCAAGAAGA TCCTGGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_849758.1
    CDS1..5994
    Translation

    Target ORF information:

    RefSeq Version XM_844665.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_844665.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGTCAGGCT CCTCCGGGGT GGCCGTGTGC TGTGCAATGG GGGCCCCTCC
    TCTCACCCCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAAGCGGCCA
    GACACGAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CTTGTGGCAC
    ACATACCGCT GGCTGCTCCT CAAACTGCTG CTGCTCATGC TGCTGCTCCT CCTCCTCGCC
    CTCTTCCTCT ACTCTGTGCC CGGCTACCTG GTCAAGAAGA TCCTGGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18752
    Clone ID Related Accession (Same CDS sequence) XM_005630229.2
    Accession Version XM_005630229.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630229.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGTCAGGCT CCTCCGGGGT GGCCGTGTGC TGTGCAATGG GGGCCCCTCC
    TCTCACCCCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCT
    GACACCGCCT TTGTCTGGTT CCTCAACCCC CTCAAGTCCA TCAAGTACCT CATCTGCACG
    CGATACAAGT GGCTCATCAT CAAGATCGTG CTGGCGCTGC TGGGGCTGCT TATGCTTGCC
    CTCTTCCTCT ACAGCCTCCC CGGCTACATG GTCAAGAAGC TCCTCGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630286.1
    CDS1..5994
    Translation

    Target ORF information:

    RefSeq Version XM_005630229.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630229.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGTCAGGCT CCTCCGGGGT GGCCGTGTGC TGTGCAATGG GGGCCCCTCC
    TCTCACCCCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCT
    GACACCGCCT TTGTCTGGTT CCTCAACCCC CTCAAGTCCA TCAAGTACCT CATCTGCACG
    CGATACAAGT GGCTCATCAT CAAGATCGTG CTGGCGCTGC TGGGGCTGCT TATGCTTGCC
    CTCTTCCTCT ACAGCCTCCC CGGCTACATG GTCAAGAAGC TCCTCGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18753
    Clone ID Related Accession (Same CDS sequence) XM_005630230.2
    Accession Version XM_005630230.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product otoferlin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAAGC GGCCAGACAC GAGCTTCATC
    TGGTTCTTGA ACCCTCTCAA GTCTGCCCGC TACTTCTTGT GGCACACATA CCGCTGGCTG
    CTCCTCAAAC TGCTGCTGCT CATGCTGCTG CTCCTCCTCC TCGCCCTCTT CCTCTACTCT
    GTGCCCGGCT ACCTGGTCAA GAAGATCCTG GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630287.1
    CDS369..6347
    Translation

    Target ORF information:

    RefSeq Version XM_005630230.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630230.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAAGC GGCCAGACAC GAGCTTCATC
    TGGTTCTTGA ACCCTCTCAA GTCTGCCCGC TACTTCTTGT GGCACACATA CCGCTGGCTG
    CTCCTCAAAC TGCTGCTGCT CATGCTGCTG CTCCTCCTCC TCGCCCTCTT CCTCTACTCT
    GTGCCCGGCT ACCTGGTCAA GAAGATCCTG GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18754
    Clone ID Related Accession (Same CDS sequence) XM_005630231.2
    Accession Version XM_005630231.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630231.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAACC GGCCTGACAC CGCCTTTGTC
    TGGTTCCTCA ACCCCCTCAA GTCCATCAAG TACCTCATCT GCACGCGATA CAAGTGGCTC
    ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTTATGC TTGCCCTCTT CCTCTACAGC
    CTCCCCGGCT ACATGGTCAA GAAGCTCCTC GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630288.1
    CDS1..5979
    Translation

    Target ORF information:

    RefSeq Version XM_005630231.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630231.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAACC GGCCTGACAC CGCCTTTGTC
    TGGTTCCTCA ACCCCCTCAA GTCCATCAAG TACCTCATCT GCACGCGATA CAAGTGGCTC
    ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTTATGC TTGCCCTCTT CCTCTACAGC
    CTCCCCGGCT ACATGGTCAA GAAGCTCCTC GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18755
    Clone ID Related Accession (Same CDS sequence) XM_005630232.2 , XM_022404547.1
    Accession Version XM_005630232.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product otoferlin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630232.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAAGCGGCCA
    GACACGAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CTTGTGGCAC
    ACATACCGCT GGCTGCTCCT CAAACTGCTG CTGCTCATGC TGCTGCTCCT CCTCCTCGCC
    CTCTTCCTCT ACTCTGTGCC CGGCTACCTG GTCAAGAAGA TCCTGGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630289.1
    CDS1..5934
    Translation

    Target ORF information:

    RefSeq Version XM_005630232.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630232.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAAGCGGCCA
    GACACGAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CTTGTGGCAC
    ACATACCGCT GGCTGCTCCT CAAACTGCTG CTGCTCATGC TGCTGCTCCT CCTCCTCGCC
    CTCTTCCTCT ACTCTGTGCC CGGCTACCTG GTCAAGAAGA TCCTGGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18755
    Clone ID Related Accession (Same CDS sequence) XM_005630232.2 , XM_022404547.1
    Accession Version XM_022404547.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAAGCGGCCA
    GACACGAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CTTGTGGCAC
    ACATACCGCT GGCTGCTCCT CAAACTGCTG CTGCTCATGC TGCTGCTCCT CCTCCTCGCC
    CTCTTCCTCT ACTCTGTGCC CGGCTACCTG GTCAAGAAGA TCCTGGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260255.1
    CDS1..5934
    Translation

    Target ORF information:

    RefSeq Version XM_022404547.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404547.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAAGCGGCCA
    GACACGAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CTTGTGGCAC
    ACATACCGCT GGCTGCTCCT CAAACTGCTG CTGCTCATGC TGCTGCTCCT CCTCCTCGCC
    CTCTTCCTCT ACTCTGTGCC CGGCTACCTG GTCAAGAAGA TCCTGGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18756
    Clone ID Related Accession (Same CDS sequence) XM_005630233.2 , XM_005630234.2 , XM_022404548.1
    Accession Version XM_005630233.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product otoferlin isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630233.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGTGCTGC AGAAGGTGGT GGAGGAGAAC CACGTGGAGG TGTCCGACAC GCTGATTGAT 
    GACAACAATG CTATCATCAA GACCAGCCTG TGCGTAGAGG TGCGGTATCA GGCCACAGAC
    GGCACAGTGG GCTCCTGGGA TGATGGGGAC TTCCTGGGAG ACGAATCCCT CCATGAAGAA
    GAGAAGGACA GCCAGGAGAC AGATGGGCTG CTCCCTGGCT CCCGCCCCAG CTCCCGGACT
    CCAGGCGAGA AGAGCTTCCG GAGCAAAGGC AGAGAGAAGA CCAAGGGAGG CAAAGATGGC
    GAGCACAAAG CCGGGAGAAG TGTGTTCTCT GCCATGAAGC TCGGCAAGAA CCGGACCCAC
    AAGGAGGAGC CGCAGAGACA AGATGAACCG GCTGTGCTGG AGATGGAGGA CCTTGACCAC
    TTGGCCATTC GGCTGGGGGA TGGGCTGGAC CCCGATTCTG TGTCTCTAGC CTCTGTCACA
    GCTCTCACCA CCAACGTCTC CAACAAGAGG TCCAAGCCAG ACATTAAGAT GGAGCCATGT
    GCTGGGCGGC CCATGGATTA CCAGGTCAGC ATCACTGTGA TCGAGGCCCG GCAGCTCGTG
    GGCTTGAACA TGGACCCCGT GGTGTGCGTG GAGGTGGGTG ATGACAAGAA GTGCACATCC
    ATGAAGGAGT CCACTAACTG CCCCTACTAC AATGAGTACT TTGTCTTCGA CTTCCACGTC
    TCTCCTGACG TTATGTTCGA CAAGATCATC AAGATCTCGG TGATTCACTC CAAGAACCTG
    CTGCGAAGTG GTACCCTAGT GGGGTCCTTC AAAATGGATG TGGGAACCGT GTACTCCCAG
    CCTGAGCACC AGTTCCACCA CAAGTGGGCC ATCCTGTCAG ACCCCGACGA CATCTCCGCT
    GGGCTGAAGG GCTACGTGAA GTGTGACATC GCTGTGGTGG GCAAGGGGGA CAACATCAAG
    ACACCCCACA AGGCCAACGA GACAGATGAG GATGACATTG AGGGGAACTT GCTGCTGCCC
    GAGGGGGTGC CTGCTGAACG CCAGTGGGCC CGGTTCTACG TGAAAATTTA CCGAGCTGAG
    GGGCTGCCCC GTATGAACAC CAGCCTCATG GCCAATGTGA AGAAGGCTTT CATTGGTGAG
    AACAAGGACC TGGTCGACCC CTATGTGCAA GTCTTCTTTG CTGGCCAGAA GGGCAAGACT
    TCAGTGCAGA AAAGTAGCTA CGAGCCCCTC TGGAATGAGC AGGTCATCTT TACAGACCTG
    TTCCCCCCAC TCTGCAAGCG CATGAAGGTG CAGATCCGAG ACTCAGACAA GGTCAACGAT
    GTGGCCATCG GCACCCACTT CATTGACCTG CGCAAGATTT CCAATGACGG AGACAAAGGC
    TTCCTGCCCA CGCTGGGCCC GGCCTGGGTG AACATGTACG GCTCCACCCG CAACTACACG
    CTGCTGGACG AGCACCAGGA CCTGAATGAG GGCCTGGGCG AGGGCGTGTC GTTCCGCGCC
    CGCCTCATGC TGGGCCTGGC TGTGGAGATC CTGGACACCT CCAACCCCGA GCTCACCAGC
    TCCACGGAGG TACAGGTGGA GCAGGCCACG CCCGTCTCGG AGAGCTGCAC AGGAAAAATG
    GAAGAATTCT TTCTATTTGG AGCCTTCCTA GAGGCCTCAA TGATCGACCG GAAAAACGGA
    GACAAGCCAA TCACCTTTGA GGTCACCATA GGTAACTATG GGAATGAAGT TGATGGCCTG
    TCGAGGCCCC AGCGGGCTCG GCTTCGGAAG GAGCCTGGGG ACGAAGAAGA GGTCGATCTG
    ATCCAGAACT CAAGTGATGA TGAAGCTGAT GAAGGTGGGG ACCTGGCCTC CGTGTCCTCC
    ACCCCTCCCA TGCGGCCCCA GATCACAGAC AGGAACTACT TCCATCTACC CTACCTGGAG
    CGCAAGCCCT GCATCTACAT GAAGAGCTGG TGGCCCGACC AGCGCCGCCG CCTCTACAAT
    GCCAACATTA TGGATCGCAT TGCAGACAAG CTGGAAGAAG GCCTGAATGA CGTGCAGGAG
    ATGATCAAGA CTGAGAAGTC TTACCCTGAG CGTCGCCTCC GGGGTGTCCT GGAGGAGCTC
    AGCTGTGGCT GCTACCGCTT CCTCTCCCTC GCCGACAAGG ACCAGGGCCA CTCATCCCGC
    ACCAGGCTGG ACCGGGAGCG CCTCAAGTCC TGCATGCGGG AGCTGGAAAG CATGGGACAG
    CAGGCCAAGA CCCTGCGGGC TCAGGTGAAG AAGCACACGG TGCGGGACAA GCTGAGGCTA
    TGCCAGAATT TCCTGCAGAA GCTGCGCTTC CTGGCAGATG AGCCCCAGCA CAGCATCCCC
    GATGTCTTCA TCTGGATGAT GAGCAATAAC AAGCGCATTG CCTACGCCCG GGTGCCCTCC
    AAGGACCTGC TCTTCTCCAT CGTTGAGGAG GAGCTGGGAA AGGACTGCGC CAAGGTCAAG
    ACGCTCTTCC TCAAGCTGCC AGGCAAGCGG GGCTTCGGCT CAGCAGGCTG GACGGTGCAG
    GCCAAGCTGG AGCTCTACCT GTGGCTGGGC CTCAGCAAGC AGCGCAAGGA CTTCCTGTGC
    GGGCTGCCCT GTGGCTTCGA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCACTCCTTC
    CCACCCATCA GCCTGGTCTA CACCAAGAAG CAGGCCTTCC AGCTCCGAGC CCACATGTAC
    CAGGCTCGCA GCCTCTTTGC TGCTGACAGC AGTGGCCTCT CAGACCCCTT CGCCCGCGTC
    TTCTTCATCA ACCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG CCCCACCTGG
    GACCAGATGC TGGTATTTGA CAACCTGGAG CTTTATGGTG AAGCCCATGA GTTGAGGGAT
    GATCCCCCTA TCATTGTCAT CGAGATCTAT GACCAGGACG CCATGGGCAA AGCCGACTTC
    ATGGGCCGGA CCTTTGCCAA GCCCCTGGTG AAGATGGCCG ACGAGGCGTA CTGCCCACCC
    CGCTTCCCAC CCCAGCTCGA GTACTACCAA ATCTACAGAG GCAATGCCAC GGCTGGGGAT
    CTGCTGGCTG CCTTCGAGCT GCTGCAGATT GGGCCAGCAG GGAAGGCAGA CCTGCCACCC
    ATCAACGGCC CTGTGGACAT GGACCGAGGT CCCATCATGC CCGTGCCTGT GGGCATCCGG
    CCCGTGCTCA GCAAATACCG AATCGAGGTG CTCTTCTGGG GCCTGAGAGA CCTGAAGCGG
    GTGAACCTGG CCCAGGTGGA CCGGCCGCGG GTGGACATCG AGTGTGCAGG AAAGGGAGTG
    CAGTCGTCCC TGATCCACAA TTACAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG
    TTTGAAGTGG ACCTCCCAGA AAACGAGCTG TTGCACCCGC CCCTGAACAT CCGAGTGGTG
    GACTGCCGGG CCTTCGGCCG CTACACCCTG GTGGGCTCCC ATGCCGTCAG CGCTCTGCGG
    CGATTCATCT ACAGGCCCCC AGACCGCTCA GCCCCCAACT GGAACACCAC AGTCAGGCTC
    CTCCGGGGTG GCCGTGTGCT GTGCAATGGG GGCCCCTCCT CTCACCCCAC AGGGGAAGTC
    GTGGTGAACA TGGAGCCAGA GGTGCCTGTC AAGAAGCTGG AGACCATGGT GAAGCTGGAC
    GCGACTTCTG ATGCTGTCAT CAAGGTGGAT GTGGCTGACG AGGAGAAGGA GAAGAAAAAG
    AAGAAGAAGG GCAACGCAGA AGAGCCAGAG GAAGAGGAGC CAGATGAGAG CATGCTGGAC
    TGGTGGTCCA AGTACTTTGC TTCCATCGAT ACCATGAAGG AGCAACTTCG GCAGCAAGAG
    GTCTCAGGCA CTGACTTGGA GGAGAAAGAG GAGGTGGACA ACACCGAGGG CCTGAAGGGA
    CCGATGAAGG TCAAGGAGAA GGCAAAGGCA GCAAAGGAAG AGAAGAAAAA GAGAACCCAG
    GGCCCCGGCG CCGGCCAGGG GTCTGAAGTC CCTGAGAAGA AGAAACCCAA GATTGATGAG
    CTGAAGGTGT ACTCCAAGGA GCTGGAGTCC GAGTTCGACA ACTTTGAGGA CTGGCTGCAC
    ACTTTCAACC TGCTGCGGGG CAAGACGGGA GATGATGAGG ACGGCTCCAC TGAGGAGGAG
    CGCATCGTGG GCAGGTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAT
    GTGTCCCGGG AAGCCGGCTA CGACCCCACC TATGGCATGT TCCAGGGCAT TCCCAGTAAT
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCAGGG CCACGGACCT GCACCCCGCA
    GACATCAACG GCAAAGCAGA CCCCTACATC GCCATCCGGC TGGGCAAGAC AGACATCCGG
    GACAAGGAGA ACTACATCTC TAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATT
    GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCCG TGTACGACTG GGACCTGGTC
    GGCACCGACG ACCTCATTGG GGAAACCAAG ATTGACCTGG AAAACCGCTT CTACAGCAAG
    CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCATAC ATGGCTACAA TATCTGGCGG
    GACCCCATGA AGCCCAGCCA GATTCTGACC CGTCTCTGCA AGGAGGGCAA AGTGGATGGC
    CCCCACTTTG GGCCCCCCGG GAGAGTGAAA GTGTCCAACC GCGTCTTCAC TGGGCCCTCC
    GAGATCGAGG ACGAGAATGG TCAGAGGAAG CCCACAGAAG AGCACGTGGC ACTGTCTGCC
    CTAAGGCACT GGGAGGACAT CCCCCGTGTG GGCTGCCGCC TCGTGCCTGA ACATGTGGAG
    ACTCGGCCGC TGCTCAACCC TGACAAGCCA GGCATCGAGC AGGGCCGCCT GGAGCTGTGG
    GTGGATATGT TCCCCATGGA CATGCCAGCA CCCGGCACAC CACTGGACAT CTCCCCTCGG
    AAGCCCAAGA AGTACGAGCT CCGGGTCATC GTGTGGAACA CGGACGAGGT GGTCCTGGAG
    GACGATGACT TCTTTACTGG GGAGAAGTCC AGCGACATTT TTGTGCGGGG GTGGCTGAAG
    GGCCAGCAGG AGGACAAGCA GGACACGGAT GTCCACTATC ACTCCCTCAC CGGCGAGGGC
    AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTATCTGG CCGCCGAGGA AAAGATTGTC
    ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACGG AGTACAAGAT CCCCGCGCGG
    CTCACCTTGC AGATCTGGGA CGCCGACCAC TTCTCCGCGG ACGATTTCCT GGGGGCCATC
    GAGCTGGACC TGAACCGGTT CCCCCGGGGC GCGAAGACAG CCAAGCAGTG CACCATGGAG
    ATGGCCACGG GGGAGGTGGA CGTGCCCCTG GTTTCCATCT TCAAGCAGAA GCGCGTCAAA
    GGCTGGTGGC CGCTCCTGGC CCGCGATGAG AATGATGAGT TCGAGCTCAC GGGCAAGGTG
    GAGGCAGAGT TGCATCTCCT GACAGCCGAG GAGGCAGAGA AGAACCCCGT GGGCCTGGCC
    CGCAATGAAC CTGACCCCCT AGAGAAACCC AAGCGGCCAG ACACGAGCTT CATCTGGTTC
    TTGAACCCTC TCAAGTCTGC CCGCTACTTC TTGTGGCACA CATACCGCTG GCTGCTCCTC
    AAACTGCTGC TGCTCATGCT GCTGCTCCTC CTCCTCGCCC TCTTCCTCTA CTCTGTGCCC
    GGCTACCTGG TCAAGAAGAT CCTGGGGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630290.1
    CDS95..5887
    Translation

    Target ORF information:

    RefSeq Version XM_005630233.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630233.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGTGCTGC AGAAGGTGGT GGAGGAGAAC CACGTGGAGG TGTCCGACAC GCTGATTGAT 
    GACAACAATG CTATCATCAA GACCAGCCTG TGCGTAGAGG TGCGGTATCA GGCCACAGAC
    GGCACAGTGG GCTCCTGGGA TGATGGGGAC TTCCTGGGAG ACGAATCCCT CCATGAAGAA
    GAGAAGGACA GCCAGGAGAC AGATGGGCTG CTCCCTGGCT CCCGCCCCAG CTCCCGGACT
    CCAGGCGAGA AGAGCTTCCG GAGCAAAGGC AGAGAGAAGA CCAAGGGAGG CAAAGATGGC
    GAGCACAAAG CCGGGAGAAG TGTGTTCTCT GCCATGAAGC TCGGCAAGAA CCGGACCCAC
    AAGGAGGAGC CGCAGAGACA AGATGAACCG GCTGTGCTGG AGATGGAGGA CCTTGACCAC
    TTGGCCATTC GGCTGGGGGA TGGGCTGGAC CCCGATTCTG TGTCTCTAGC CTCTGTCACA
    GCTCTCACCA CCAACGTCTC CAACAAGAGG TCCAAGCCAG ACATTAAGAT GGAGCCATGT
    GCTGGGCGGC CCATGGATTA CCAGGTCAGC ATCACTGTGA TCGAGGCCCG GCAGCTCGTG
    GGCTTGAACA TGGACCCCGT GGTGTGCGTG GAGGTGGGTG ATGACAAGAA GTGCACATCC
    ATGAAGGAGT CCACTAACTG CCCCTACTAC AATGAGTACT TTGTCTTCGA CTTCCACGTC
    TCTCCTGACG TTATGTTCGA CAAGATCATC AAGATCTCGG TGATTCACTC CAAGAACCTG
    CTGCGAAGTG GTACCCTAGT GGGGTCCTTC AAAATGGATG TGGGAACCGT GTACTCCCAG
    CCTGAGCACC AGTTCCACCA CAAGTGGGCC ATCCTGTCAG ACCCCGACGA CATCTCCGCT
    GGGCTGAAGG GCTACGTGAA GTGTGACATC GCTGTGGTGG GCAAGGGGGA CAACATCAAG
    ACACCCCACA AGGCCAACGA GACAGATGAG GATGACATTG AGGGGAACTT GCTGCTGCCC
    GAGGGGGTGC CTGCTGAACG CCAGTGGGCC CGGTTCTACG TGAAAATTTA CCGAGCTGAG
    GGGCTGCCCC GTATGAACAC CAGCCTCATG GCCAATGTGA AGAAGGCTTT CATTGGTGAG
    AACAAGGACC TGGTCGACCC CTATGTGCAA GTCTTCTTTG CTGGCCAGAA GGGCAAGACT
    TCAGTGCAGA AAAGTAGCTA CGAGCCCCTC TGGAATGAGC AGGTCATCTT TACAGACCTG
    TTCCCCCCAC TCTGCAAGCG CATGAAGGTG CAGATCCGAG ACTCAGACAA GGTCAACGAT
    GTGGCCATCG GCACCCACTT CATTGACCTG CGCAAGATTT CCAATGACGG AGACAAAGGC
    TTCCTGCCCA CGCTGGGCCC GGCCTGGGTG AACATGTACG GCTCCACCCG CAACTACACG
    CTGCTGGACG AGCACCAGGA CCTGAATGAG GGCCTGGGCG AGGGCGTGTC GTTCCGCGCC
    CGCCTCATGC TGGGCCTGGC TGTGGAGATC CTGGACACCT CCAACCCCGA GCTCACCAGC
    TCCACGGAGG TACAGGTGGA GCAGGCCACG CCCGTCTCGG AGAGCTGCAC AGGAAAAATG
    GAAGAATTCT TTCTATTTGG AGCCTTCCTA GAGGCCTCAA TGATCGACCG GAAAAACGGA
    GACAAGCCAA TCACCTTTGA GGTCACCATA GGTAACTATG GGAATGAAGT TGATGGCCTG
    TCGAGGCCCC AGCGGGCTCG GCTTCGGAAG GAGCCTGGGG ACGAAGAAGA GGTCGATCTG
    ATCCAGAACT CAAGTGATGA TGAAGCTGAT GAAGGTGGGG ACCTGGCCTC CGTGTCCTCC
    ACCCCTCCCA TGCGGCCCCA GATCACAGAC AGGAACTACT TCCATCTACC CTACCTGGAG
    CGCAAGCCCT GCATCTACAT GAAGAGCTGG TGGCCCGACC AGCGCCGCCG CCTCTACAAT
    GCCAACATTA TGGATCGCAT TGCAGACAAG CTGGAAGAAG GCCTGAATGA CGTGCAGGAG
    ATGATCAAGA CTGAGAAGTC TTACCCTGAG CGTCGCCTCC GGGGTGTCCT GGAGGAGCTC
    AGCTGTGGCT GCTACCGCTT CCTCTCCCTC GCCGACAAGG ACCAGGGCCA CTCATCCCGC
    ACCAGGCTGG ACCGGGAGCG CCTCAAGTCC TGCATGCGGG AGCTGGAAAG CATGGGACAG
    CAGGCCAAGA CCCTGCGGGC TCAGGTGAAG AAGCACACGG TGCGGGACAA GCTGAGGCTA
    TGCCAGAATT TCCTGCAGAA GCTGCGCTTC CTGGCAGATG AGCCCCAGCA CAGCATCCCC
    GATGTCTTCA TCTGGATGAT GAGCAATAAC AAGCGCATTG CCTACGCCCG GGTGCCCTCC
    AAGGACCTGC TCTTCTCCAT CGTTGAGGAG GAGCTGGGAA AGGACTGCGC CAAGGTCAAG
    ACGCTCTTCC TCAAGCTGCC AGGCAAGCGG GGCTTCGGCT CAGCAGGCTG GACGGTGCAG
    GCCAAGCTGG AGCTCTACCT GTGGCTGGGC CTCAGCAAGC AGCGCAAGGA CTTCCTGTGC
    GGGCTGCCCT GTGGCTTCGA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCACTCCTTC
    CCACCCATCA GCCTGGTCTA CACCAAGAAG CAGGCCTTCC AGCTCCGAGC CCACATGTAC
    CAGGCTCGCA GCCTCTTTGC TGCTGACAGC AGTGGCCTCT CAGACCCCTT CGCCCGCGTC
    TTCTTCATCA ACCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG CCCCACCTGG
    GACCAGATGC TGGTATTTGA CAACCTGGAG CTTTATGGTG AAGCCCATGA GTTGAGGGAT
    GATCCCCCTA TCATTGTCAT CGAGATCTAT GACCAGGACG CCATGGGCAA AGCCGACTTC
    ATGGGCCGGA CCTTTGCCAA GCCCCTGGTG AAGATGGCCG ACGAGGCGTA CTGCCCACCC
    CGCTTCCCAC CCCAGCTCGA GTACTACCAA ATCTACAGAG GCAATGCCAC GGCTGGGGAT
    CTGCTGGCTG CCTTCGAGCT GCTGCAGATT GGGCCAGCAG GGAAGGCAGA CCTGCCACCC
    ATCAACGGCC CTGTGGACAT GGACCGAGGT CCCATCATGC CCGTGCCTGT GGGCATCCGG
    CCCGTGCTCA GCAAATACCG AATCGAGGTG CTCTTCTGGG GCCTGAGAGA CCTGAAGCGG
    GTGAACCTGG CCCAGGTGGA CCGGCCGCGG GTGGACATCG AGTGTGCAGG AAAGGGAGTG
    CAGTCGTCCC TGATCCACAA TTACAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG
    TTTGAAGTGG ACCTCCCAGA AAACGAGCTG TTGCACCCGC CCCTGAACAT CCGAGTGGTG
    GACTGCCGGG CCTTCGGCCG CTACACCCTG GTGGGCTCCC ATGCCGTCAG CGCTCTGCGG
    CGATTCATCT ACAGGCCCCC AGACCGCTCA GCCCCCAACT GGAACACCAC AGTCAGGCTC
    CTCCGGGGTG GCCGTGTGCT GTGCAATGGG GGCCCCTCCT CTCACCCCAC AGGGGAAGTC
    GTGGTGAACA TGGAGCCAGA GGTGCCTGTC AAGAAGCTGG AGACCATGGT GAAGCTGGAC
    GCGACTTCTG ATGCTGTCAT CAAGGTGGAT GTGGCTGACG AGGAGAAGGA GAAGAAAAAG
    AAGAAGAAGG GCAACGCAGA AGAGCCAGAG GAAGAGGAGC CAGATGAGAG CATGCTGGAC
    TGGTGGTCCA AGTACTTTGC TTCCATCGAT ACCATGAAGG AGCAACTTCG GCAGCAAGAG
    GTCTCAGGCA CTGACTTGGA GGAGAAAGAG GAGGTGGACA ACACCGAGGG CCTGAAGGGA
    CCGATGAAGG TCAAGGAGAA GGCAAAGGCA GCAAAGGAAG AGAAGAAAAA GAGAACCCAG
    GGCCCCGGCG CCGGCCAGGG GTCTGAAGTC CCTGAGAAGA AGAAACCCAA GATTGATGAG
    CTGAAGGTGT ACTCCAAGGA GCTGGAGTCC GAGTTCGACA ACTTTGAGGA CTGGCTGCAC
    ACTTTCAACC TGCTGCGGGG CAAGACGGGA GATGATGAGG ACGGCTCCAC TGAGGAGGAG
    CGCATCGTGG GCAGGTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAT
    GTGTCCCGGG AAGCCGGCTA CGACCCCACC TATGGCATGT TCCAGGGCAT TCCCAGTAAT
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCAGGG CCACGGACCT GCACCCCGCA
    GACATCAACG GCAAAGCAGA CCCCTACATC GCCATCCGGC TGGGCAAGAC AGACATCCGG
    GACAAGGAGA ACTACATCTC TAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATT
    GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCCG TGTACGACTG GGACCTGGTC
    GGCACCGACG ACCTCATTGG GGAAACCAAG ATTGACCTGG AAAACCGCTT CTACAGCAAG
    CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCATAC ATGGCTACAA TATCTGGCGG
    GACCCCATGA AGCCCAGCCA GATTCTGACC CGTCTCTGCA AGGAGGGCAA AGTGGATGGC
    CCCCACTTTG GGCCCCCCGG GAGAGTGAAA GTGTCCAACC GCGTCTTCAC TGGGCCCTCC
    GAGATCGAGG ACGAGAATGG TCAGAGGAAG CCCACAGAAG AGCACGTGGC ACTGTCTGCC
    CTAAGGCACT GGGAGGACAT CCCCCGTGTG GGCTGCCGCC TCGTGCCTGA ACATGTGGAG
    ACTCGGCCGC TGCTCAACCC TGACAAGCCA GGCATCGAGC AGGGCCGCCT GGAGCTGTGG
    GTGGATATGT TCCCCATGGA CATGCCAGCA CCCGGCACAC CACTGGACAT CTCCCCTCGG
    AAGCCCAAGA AGTACGAGCT CCGGGTCATC GTGTGGAACA CGGACGAGGT GGTCCTGGAG
    GACGATGACT TCTTTACTGG GGAGAAGTCC AGCGACATTT TTGTGCGGGG GTGGCTGAAG
    GGCCAGCAGG AGGACAAGCA GGACACGGAT GTCCACTATC ACTCCCTCAC CGGCGAGGGC
    AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTATCTGG CCGCCGAGGA AAAGATTGTC
    ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACGG AGTACAAGAT CCCCGCGCGG
    CTCACCTTGC AGATCTGGGA CGCCGACCAC TTCTCCGCGG ACGATTTCCT GGGGGCCATC
    GAGCTGGACC TGAACCGGTT CCCCCGGGGC GCGAAGACAG CCAAGCAGTG CACCATGGAG
    ATGGCCACGG GGGAGGTGGA CGTGCCCCTG GTTTCCATCT TCAAGCAGAA GCGCGTCAAA
    GGCTGGTGGC CGCTCCTGGC CCGCGATGAG AATGATGAGT TCGAGCTCAC GGGCAAGGTG
    GAGGCAGAGT TGCATCTCCT GACAGCCGAG GAGGCAGAGA AGAACCCCGT GGGCCTGGCC
    CGCAATGAAC CTGACCCCCT AGAGAAACCC AAGCGGCCAG ACACGAGCTT CATCTGGTTC
    TTGAACCCTC TCAAGTCTGC CCGCTACTTC TTGTGGCACA CATACCGCTG GCTGCTCCTC
    AAACTGCTGC TGCTCATGCT GCTGCTCCTC CTCCTCGCCC TCTTCCTCTA CTCTGTGCCC
    GGCTACCTGG TCAAGAAGAT CCTGGGGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18756
    Clone ID Related Accession (Same CDS sequence) XM_005630233.2 , XM_005630234.2 , XM_022404548.1
    Accession Version XM_005630234.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product otoferlin isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGTGCTGC AGAAGGTGGT GGAGGAGAAC CACGTGGAGG TGTCCGACAC GCTGATTGAT 
    GACAACAATG CTATCATCAA GACCAGCCTG TGCGTAGAGG TGCGGTATCA GGCCACAGAC
    GGCACAGTGG GCTCCTGGGA TGATGGGGAC TTCCTGGGAG ACGAATCCCT CCATGAAGAA
    GAGAAGGACA GCCAGGAGAC AGATGGGCTG CTCCCTGGCT CCCGCCCCAG CTCCCGGACT
    CCAGGCGAGA AGAGCTTCCG GAGCAAAGGC AGAGAGAAGA CCAAGGGAGG CAAAGATGGC
    GAGCACAAAG CCGGGAGAAG TGTGTTCTCT GCCATGAAGC TCGGCAAGAA CCGGACCCAC
    AAGGAGGAGC CGCAGAGACA AGATGAACCG GCTGTGCTGG AGATGGAGGA CCTTGACCAC
    TTGGCCATTC GGCTGGGGGA TGGGCTGGAC CCCGATTCTG TGTCTCTAGC CTCTGTCACA
    GCTCTCACCA CCAACGTCTC CAACAAGAGG TCCAAGCCAG ACATTAAGAT GGAGCCATGT
    GCTGGGCGGC CCATGGATTA CCAGGTCAGC ATCACTGTGA TCGAGGCCCG GCAGCTCGTG
    GGCTTGAACA TGGACCCCGT GGTGTGCGTG GAGGTGGGTG ATGACAAGAA GTGCACATCC
    ATGAAGGAGT CCACTAACTG CCCCTACTAC AATGAGTACT TTGTCTTCGA CTTCCACGTC
    TCTCCTGACG TTATGTTCGA CAAGATCATC AAGATCTCGG TGATTCACTC CAAGAACCTG
    CTGCGAAGTG GTACCCTAGT GGGGTCCTTC AAAATGGATG TGGGAACCGT GTACTCCCAG
    CCTGAGCACC AGTTCCACCA CAAGTGGGCC ATCCTGTCAG ACCCCGACGA CATCTCCGCT
    GGGCTGAAGG GCTACGTGAA GTGTGACATC GCTGTGGTGG GCAAGGGGGA CAACATCAAG
    ACACCCCACA AGGCCAACGA GACAGATGAG GATGACATTG AGGGGAACTT GCTGCTGCCC
    GAGGGGGTGC CTGCTGAACG CCAGTGGGCC CGGTTCTACG TGAAAATTTA CCGAGCTGAG
    GGGCTGCCCC GTATGAACAC CAGCCTCATG GCCAATGTGA AGAAGGCTTT CATTGGTGAG
    AACAAGGACC TGGTCGACCC CTATGTGCAA GTCTTCTTTG CTGGCCAGAA GGGCAAGACT
    TCAGTGCAGA AAAGTAGCTA CGAGCCCCTC TGGAATGAGC AGGTCATCTT TACAGACCTG
    TTCCCCCCAC TCTGCAAGCG CATGAAGGTG CAGATCCGAG ACTCAGACAA GGTCAACGAT
    GTGGCCATCG GCACCCACTT CATTGACCTG CGCAAGATTT CCAATGACGG AGACAAAGGC
    TTCCTGCCCA CGCTGGGCCC GGCCTGGGTG AACATGTACG GCTCCACCCG CAACTACACG
    CTGCTGGACG AGCACCAGGA CCTGAATGAG GGCCTGGGCG AGGGCGTGTC GTTCCGCGCC
    CGCCTCATGC TGGGCCTGGC TGTGGAGATC CTGGACACCT CCAACCCCGA GCTCACCAGC
    TCCACGGAGG TACAGGTGGA GCAGGCCACG CCCGTCTCGG AGAGCTGCAC AGGAAAAATG
    GAAGAATTCT TTCTATTTGG AGCCTTCCTA GAGGCCTCAA TGATCGACCG GAAAAACGGA
    GACAAGCCAA TCACCTTTGA GGTCACCATA GGTAACTATG GGAATGAAGT TGATGGCCTG
    TCGAGGCCCC AGCGGGCTCG GCTTCGGAAG GAGCCTGGGG ACGAAGAAGA GGTCGATCTG
    ATCCAGAACT CAAGTGATGA TGAAGCTGAT GAAGGTGGGG ACCTGGCCTC CGTGTCCTCC
    ACCCCTCCCA TGCGGCCCCA GATCACAGAC AGGAACTACT TCCATCTACC CTACCTGGAG
    CGCAAGCCCT GCATCTACAT GAAGAGCTGG TGGCCCGACC AGCGCCGCCG CCTCTACAAT
    GCCAACATTA TGGATCGCAT TGCAGACAAG CTGGAAGAAG GCCTGAATGA CGTGCAGGAG
    ATGATCAAGA CTGAGAAGTC TTACCCTGAG CGTCGCCTCC GGGGTGTCCT GGAGGAGCTC
    AGCTGTGGCT GCTACCGCTT CCTCTCCCTC GCCGACAAGG ACCAGGGCCA CTCATCCCGC
    ACCAGGCTGG ACCGGGAGCG CCTCAAGTCC TGCATGCGGG AGCTGGAAAG CATGGGACAG
    CAGGCCAAGA CCCTGCGGGC TCAGGTGAAG AAGCACACGG TGCGGGACAA GCTGAGGCTA
    TGCCAGAATT TCCTGCAGAA GCTGCGCTTC CTGGCAGATG AGCCCCAGCA CAGCATCCCC
    GATGTCTTCA TCTGGATGAT GAGCAATAAC AAGCGCATTG CCTACGCCCG GGTGCCCTCC
    AAGGACCTGC TCTTCTCCAT CGTTGAGGAG GAGCTGGGAA AGGACTGCGC CAAGGTCAAG
    ACGCTCTTCC TCAAGCTGCC AGGCAAGCGG GGCTTCGGCT CAGCAGGCTG GACGGTGCAG
    GCCAAGCTGG AGCTCTACCT GTGGCTGGGC CTCAGCAAGC AGCGCAAGGA CTTCCTGTGC
    GGGCTGCCCT GTGGCTTCGA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCACTCCTTC
    CCACCCATCA GCCTGGTCTA CACCAAGAAG CAGGCCTTCC AGCTCCGAGC CCACATGTAC
    CAGGCTCGCA GCCTCTTTGC TGCTGACAGC AGTGGCCTCT CAGACCCCTT CGCCCGCGTC
    TTCTTCATCA ACCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG CCCCACCTGG
    GACCAGATGC TGGTATTTGA CAACCTGGAG CTTTATGGTG AAGCCCATGA GTTGAGGGAT
    GATCCCCCTA TCATTGTCAT CGAGATCTAT GACCAGGACG CCATGGGCAA AGCCGACTTC
    ATGGGCCGGA CCTTTGCCAA GCCCCTGGTG AAGATGGCCG ACGAGGCGTA CTGCCCACCC
    CGCTTCCCAC CCCAGCTCGA GTACTACCAA ATCTACAGAG GCAATGCCAC GGCTGGGGAT
    CTGCTGGCTG CCTTCGAGCT GCTGCAGATT GGGCCAGCAG GGAAGGCAGA CCTGCCACCC
    ATCAACGGCC CTGTGGACAT GGACCGAGGT CCCATCATGC CCGTGCCTGT GGGCATCCGG
    CCCGTGCTCA GCAAATACCG AATCGAGGTG CTCTTCTGGG GCCTGAGAGA CCTGAAGCGG
    GTGAACCTGG CCCAGGTGGA CCGGCCGCGG GTGGACATCG AGTGTGCAGG AAAGGGAGTG
    CAGTCGTCCC TGATCCACAA TTACAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG
    TTTGAAGTGG ACCTCCCAGA AAACGAGCTG TTGCACCCGC CCCTGAACAT CCGAGTGGTG
    GACTGCCGGG CCTTCGGCCG CTACACCCTG GTGGGCTCCC ATGCCGTCAG CGCTCTGCGG
    CGATTCATCT ACAGGCCCCC AGACCGCTCA GCCCCCAACT GGAACACCAC AGTCAGGCTC
    CTCCGGGGTG GCCGTGTGCT GTGCAATGGG GGCCCCTCCT CTCACCCCAC AGGGGAAGTC
    GTGGTGAACA TGGAGCCAGA GGTGCCTGTC AAGAAGCTGG AGACCATGGT GAAGCTGGAC
    GCGACTTCTG ATGCTGTCAT CAAGGTGGAT GTGGCTGACG AGGAGAAGGA GAAGAAAAAG
    AAGAAGAAGG GCAACGCAGA AGAGCCAGAG GAAGAGGAGC CAGATGAGAG CATGCTGGAC
    TGGTGGTCCA AGTACTTTGC TTCCATCGAT ACCATGAAGG AGCAACTTCG GCAGCAAGAG
    GTCTCAGGCA CTGACTTGGA GGAGAAAGAG GAGGTGGACA ACACCGAGGG CCTGAAGGGA
    CCGATGAAGG TCAAGGAGAA GGCAAAGGCA GCAAAGGAAG AGAAGAAAAA GAGAACCCAG
    GGCCCCGGCG CCGGCCAGGG GTCTGAAGTC CCTGAGAAGA AGAAACCCAA GATTGATGAG
    CTGAAGGTGT ACTCCAAGGA GCTGGAGTCC GAGTTCGACA ACTTTGAGGA CTGGCTGCAC
    ACTTTCAACC TGCTGCGGGG CAAGACGGGA GATGATGAGG ACGGCTCCAC TGAGGAGGAG
    CGCATCGTGG GCAGGTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAT
    GTGTCCCGGG AAGCCGGCTA CGACCCCACC TATGGCATGT TCCAGGGCAT TCCCAGTAAT
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCAGGG CCACGGACCT GCACCCCGCA
    GACATCAACG GCAAAGCAGA CCCCTACATC GCCATCCGGC TGGGCAAGAC AGACATCCGG
    GACAAGGAGA ACTACATCTC TAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATT
    GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCCG TGTACGACTG GGACCTGGTC
    GGCACCGACG ACCTCATTGG GGAAACCAAG ATTGACCTGG AAAACCGCTT CTACAGCAAG
    CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCATAC ATGGCTACAA TATCTGGCGG
    GACCCCATGA AGCCCAGCCA GATTCTGACC CGTCTCTGCA AGGAGGGCAA AGTGGATGGC
    CCCCACTTTG GGCCCCCCGG GAGAGTGAAA GTGTCCAACC GCGTCTTCAC TGGGCCCTCC
    GAGATCGAGG ACGAGAATGG TCAGAGGAAG CCCACAGAAG AGCACGTGGC ACTGTCTGCC
    CTAAGGCACT GGGAGGACAT CCCCCGTGTG GGCTGCCGCC TCGTGCCTGA ACATGTGGAG
    ACTCGGCCGC TGCTCAACCC TGACAAGCCA GGCATCGAGC AGGGCCGCCT GGAGCTGTGG
    GTGGATATGT TCCCCATGGA CATGCCAGCA CCCGGCACAC CACTGGACAT CTCCCCTCGG
    AAGCCCAAGA AGTACGAGCT CCGGGTCATC GTGTGGAACA CGGACGAGGT GGTCCTGGAG
    GACGATGACT TCTTTACTGG GGAGAAGTCC AGCGACATTT TTGTGCGGGG GTGGCTGAAG
    GGCCAGCAGG AGGACAAGCA GGACACGGAT GTCCACTATC ACTCCCTCAC CGGCGAGGGC
    AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTATCTGG CCGCCGAGGA AAAGATTGTC
    ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACGG AGTACAAGAT CCCCGCGCGG
    CTCACCTTGC AGATCTGGGA CGCCGACCAC TTCTCCGCGG ACGATTTCCT GGGGGCCATC
    GAGCTGGACC TGAACCGGTT CCCCCGGGGC GCGAAGACAG CCAAGCAGTG CACCATGGAG
    ATGGCCACGG GGGAGGTGGA CGTGCCCCTG GTTTCCATCT TCAAGCAGAA GCGCGTCAAA
    GGCTGGTGGC CGCTCCTGGC CCGCGATGAG AATGATGAGT TCGAGCTCAC GGGCAAGGTG
    GAGGCAGAGT TGCATCTCCT GACAGCCGAG GAGGCAGAGA AGAACCCCGT GGGCCTGGCC
    CGCAATGAAC CTGACCCCCT AGAGAAACCC AAGCGGCCAG ACACGAGCTT CATCTGGTTC
    TTGAACCCTC TCAAGTCTGC CCGCTACTTC TTGTGGCACA CATACCGCTG GCTGCTCCTC
    AAACTGCTGC TGCTCATGCT GCTGCTCCTC CTCCTCGCCC TCTTCCTCTA CTCTGTGCCC
    GGCTACCTGG TCAAGAAGAT CCTGGGGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630291.1
    CDS300..6092
    Translation

    Target ORF information:

    RefSeq Version XM_005630234.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630234.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGTGCTGC AGAAGGTGGT GGAGGAGAAC CACGTGGAGG TGTCCGACAC GCTGATTGAT 
    GACAACAATG CTATCATCAA GACCAGCCTG TGCGTAGAGG TGCGGTATCA GGCCACAGAC
    GGCACAGTGG GCTCCTGGGA TGATGGGGAC TTCCTGGGAG ACGAATCCCT CCATGAAGAA
    GAGAAGGACA GCCAGGAGAC AGATGGGCTG CTCCCTGGCT CCCGCCCCAG CTCCCGGACT
    CCAGGCGAGA AGAGCTTCCG GAGCAAAGGC AGAGAGAAGA CCAAGGGAGG CAAAGATGGC
    GAGCACAAAG CCGGGAGAAG TGTGTTCTCT GCCATGAAGC TCGGCAAGAA CCGGACCCAC
    AAGGAGGAGC CGCAGAGACA AGATGAACCG GCTGTGCTGG AGATGGAGGA CCTTGACCAC
    TTGGCCATTC GGCTGGGGGA TGGGCTGGAC CCCGATTCTG TGTCTCTAGC CTCTGTCACA
    GCTCTCACCA CCAACGTCTC CAACAAGAGG TCCAAGCCAG ACATTAAGAT GGAGCCATGT
    GCTGGGCGGC CCATGGATTA CCAGGTCAGC ATCACTGTGA TCGAGGCCCG GCAGCTCGTG
    GGCTTGAACA TGGACCCCGT GGTGTGCGTG GAGGTGGGTG ATGACAAGAA GTGCACATCC
    ATGAAGGAGT CCACTAACTG CCCCTACTAC AATGAGTACT TTGTCTTCGA CTTCCACGTC
    TCTCCTGACG TTATGTTCGA CAAGATCATC AAGATCTCGG TGATTCACTC CAAGAACCTG
    CTGCGAAGTG GTACCCTAGT GGGGTCCTTC AAAATGGATG TGGGAACCGT GTACTCCCAG
    CCTGAGCACC AGTTCCACCA CAAGTGGGCC ATCCTGTCAG ACCCCGACGA CATCTCCGCT
    GGGCTGAAGG GCTACGTGAA GTGTGACATC GCTGTGGTGG GCAAGGGGGA CAACATCAAG
    ACACCCCACA AGGCCAACGA GACAGATGAG GATGACATTG AGGGGAACTT GCTGCTGCCC
    GAGGGGGTGC CTGCTGAACG CCAGTGGGCC CGGTTCTACG TGAAAATTTA CCGAGCTGAG
    GGGCTGCCCC GTATGAACAC CAGCCTCATG GCCAATGTGA AGAAGGCTTT CATTGGTGAG
    AACAAGGACC TGGTCGACCC CTATGTGCAA GTCTTCTTTG CTGGCCAGAA GGGCAAGACT
    TCAGTGCAGA AAAGTAGCTA CGAGCCCCTC TGGAATGAGC AGGTCATCTT TACAGACCTG
    TTCCCCCCAC TCTGCAAGCG CATGAAGGTG CAGATCCGAG ACTCAGACAA GGTCAACGAT
    GTGGCCATCG GCACCCACTT CATTGACCTG CGCAAGATTT CCAATGACGG AGACAAAGGC
    TTCCTGCCCA CGCTGGGCCC GGCCTGGGTG AACATGTACG GCTCCACCCG CAACTACACG
    CTGCTGGACG AGCACCAGGA CCTGAATGAG GGCCTGGGCG AGGGCGTGTC GTTCCGCGCC
    CGCCTCATGC TGGGCCTGGC TGTGGAGATC CTGGACACCT CCAACCCCGA GCTCACCAGC
    TCCACGGAGG TACAGGTGGA GCAGGCCACG CCCGTCTCGG AGAGCTGCAC AGGAAAAATG
    GAAGAATTCT TTCTATTTGG AGCCTTCCTA GAGGCCTCAA TGATCGACCG GAAAAACGGA
    GACAAGCCAA TCACCTTTGA GGTCACCATA GGTAACTATG GGAATGAAGT TGATGGCCTG
    TCGAGGCCCC AGCGGGCTCG GCTTCGGAAG GAGCCTGGGG ACGAAGAAGA GGTCGATCTG
    ATCCAGAACT CAAGTGATGA TGAAGCTGAT GAAGGTGGGG ACCTGGCCTC CGTGTCCTCC
    ACCCCTCCCA TGCGGCCCCA GATCACAGAC AGGAACTACT TCCATCTACC CTACCTGGAG
    CGCAAGCCCT GCATCTACAT GAAGAGCTGG TGGCCCGACC AGCGCCGCCG CCTCTACAAT
    GCCAACATTA TGGATCGCAT TGCAGACAAG CTGGAAGAAG GCCTGAATGA CGTGCAGGAG
    ATGATCAAGA CTGAGAAGTC TTACCCTGAG CGTCGCCTCC GGGGTGTCCT GGAGGAGCTC
    AGCTGTGGCT GCTACCGCTT CCTCTCCCTC GCCGACAAGG ACCAGGGCCA CTCATCCCGC
    ACCAGGCTGG ACCGGGAGCG CCTCAAGTCC TGCATGCGGG AGCTGGAAAG CATGGGACAG
    CAGGCCAAGA CCCTGCGGGC TCAGGTGAAG AAGCACACGG TGCGGGACAA GCTGAGGCTA
    TGCCAGAATT TCCTGCAGAA GCTGCGCTTC CTGGCAGATG AGCCCCAGCA CAGCATCCCC
    GATGTCTTCA TCTGGATGAT GAGCAATAAC AAGCGCATTG CCTACGCCCG GGTGCCCTCC
    AAGGACCTGC TCTTCTCCAT CGTTGAGGAG GAGCTGGGAA AGGACTGCGC CAAGGTCAAG
    ACGCTCTTCC TCAAGCTGCC AGGCAAGCGG GGCTTCGGCT CAGCAGGCTG GACGGTGCAG
    GCCAAGCTGG AGCTCTACCT GTGGCTGGGC CTCAGCAAGC AGCGCAAGGA CTTCCTGTGC
    GGGCTGCCCT GTGGCTTCGA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCACTCCTTC
    CCACCCATCA GCCTGGTCTA CACCAAGAAG CAGGCCTTCC AGCTCCGAGC CCACATGTAC
    CAGGCTCGCA GCCTCTTTGC TGCTGACAGC AGTGGCCTCT CAGACCCCTT CGCCCGCGTC
    TTCTTCATCA ACCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG CCCCACCTGG
    GACCAGATGC TGGTATTTGA CAACCTGGAG CTTTATGGTG AAGCCCATGA GTTGAGGGAT
    GATCCCCCTA TCATTGTCAT CGAGATCTAT GACCAGGACG CCATGGGCAA AGCCGACTTC
    ATGGGCCGGA CCTTTGCCAA GCCCCTGGTG AAGATGGCCG ACGAGGCGTA CTGCCCACCC
    CGCTTCCCAC CCCAGCTCGA GTACTACCAA ATCTACAGAG GCAATGCCAC GGCTGGGGAT
    CTGCTGGCTG CCTTCGAGCT GCTGCAGATT GGGCCAGCAG GGAAGGCAGA CCTGCCACCC
    ATCAACGGCC CTGTGGACAT GGACCGAGGT CCCATCATGC CCGTGCCTGT GGGCATCCGG
    CCCGTGCTCA GCAAATACCG AATCGAGGTG CTCTTCTGGG GCCTGAGAGA CCTGAAGCGG
    GTGAACCTGG CCCAGGTGGA CCGGCCGCGG GTGGACATCG AGTGTGCAGG AAAGGGAGTG
    CAGTCGTCCC TGATCCACAA TTACAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG
    TTTGAAGTGG ACCTCCCAGA AAACGAGCTG TTGCACCCGC CCCTGAACAT CCGAGTGGTG
    GACTGCCGGG CCTTCGGCCG CTACACCCTG GTGGGCTCCC ATGCCGTCAG CGCTCTGCGG
    CGATTCATCT ACAGGCCCCC AGACCGCTCA GCCCCCAACT GGAACACCAC AGTCAGGCTC
    CTCCGGGGTG GCCGTGTGCT GTGCAATGGG GGCCCCTCCT CTCACCCCAC AGGGGAAGTC
    GTGGTGAACA TGGAGCCAGA GGTGCCTGTC AAGAAGCTGG AGACCATGGT GAAGCTGGAC
    GCGACTTCTG ATGCTGTCAT CAAGGTGGAT GTGGCTGACG AGGAGAAGGA GAAGAAAAAG
    AAGAAGAAGG GCAACGCAGA AGAGCCAGAG GAAGAGGAGC CAGATGAGAG CATGCTGGAC
    TGGTGGTCCA AGTACTTTGC TTCCATCGAT ACCATGAAGG AGCAACTTCG GCAGCAAGAG
    GTCTCAGGCA CTGACTTGGA GGAGAAAGAG GAGGTGGACA ACACCGAGGG CCTGAAGGGA
    CCGATGAAGG TCAAGGAGAA GGCAAAGGCA GCAAAGGAAG AGAAGAAAAA GAGAACCCAG
    GGCCCCGGCG CCGGCCAGGG GTCTGAAGTC CCTGAGAAGA AGAAACCCAA GATTGATGAG
    CTGAAGGTGT ACTCCAAGGA GCTGGAGTCC GAGTTCGACA ACTTTGAGGA CTGGCTGCAC
    ACTTTCAACC TGCTGCGGGG CAAGACGGGA GATGATGAGG ACGGCTCCAC TGAGGAGGAG
    CGCATCGTGG GCAGGTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAT
    GTGTCCCGGG AAGCCGGCTA CGACCCCACC TATGGCATGT TCCAGGGCAT TCCCAGTAAT
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCAGGG CCACGGACCT GCACCCCGCA
    GACATCAACG GCAAAGCAGA CCCCTACATC GCCATCCGGC TGGGCAAGAC AGACATCCGG
    GACAAGGAGA ACTACATCTC TAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATT
    GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCCG TGTACGACTG GGACCTGGTC
    GGCACCGACG ACCTCATTGG GGAAACCAAG ATTGACCTGG AAAACCGCTT CTACAGCAAG
    CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCATAC ATGGCTACAA TATCTGGCGG
    GACCCCATGA AGCCCAGCCA GATTCTGACC CGTCTCTGCA AGGAGGGCAA AGTGGATGGC
    CCCCACTTTG GGCCCCCCGG GAGAGTGAAA GTGTCCAACC GCGTCTTCAC TGGGCCCTCC
    GAGATCGAGG ACGAGAATGG TCAGAGGAAG CCCACAGAAG AGCACGTGGC ACTGTCTGCC
    CTAAGGCACT GGGAGGACAT CCCCCGTGTG GGCTGCCGCC TCGTGCCTGA ACATGTGGAG
    ACTCGGCCGC TGCTCAACCC TGACAAGCCA GGCATCGAGC AGGGCCGCCT GGAGCTGTGG
    GTGGATATGT TCCCCATGGA CATGCCAGCA CCCGGCACAC CACTGGACAT CTCCCCTCGG
    AAGCCCAAGA AGTACGAGCT CCGGGTCATC GTGTGGAACA CGGACGAGGT GGTCCTGGAG
    GACGATGACT TCTTTACTGG GGAGAAGTCC AGCGACATTT TTGTGCGGGG GTGGCTGAAG
    GGCCAGCAGG AGGACAAGCA GGACACGGAT GTCCACTATC ACTCCCTCAC CGGCGAGGGC
    AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTATCTGG CCGCCGAGGA AAAGATTGTC
    ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACGG AGTACAAGAT CCCCGCGCGG
    CTCACCTTGC AGATCTGGGA CGCCGACCAC TTCTCCGCGG ACGATTTCCT GGGGGCCATC
    GAGCTGGACC TGAACCGGTT CCCCCGGGGC GCGAAGACAG CCAAGCAGTG CACCATGGAG
    ATGGCCACGG GGGAGGTGGA CGTGCCCCTG GTTTCCATCT TCAAGCAGAA GCGCGTCAAA
    GGCTGGTGGC CGCTCCTGGC CCGCGATGAG AATGATGAGT TCGAGCTCAC GGGCAAGGTG
    GAGGCAGAGT TGCATCTCCT GACAGCCGAG GAGGCAGAGA AGAACCCCGT GGGCCTGGCC
    CGCAATGAAC CTGACCCCCT AGAGAAACCC AAGCGGCCAG ACACGAGCTT CATCTGGTTC
    TTGAACCCTC TCAAGTCTGC CCGCTACTTC TTGTGGCACA CATACCGCTG GCTGCTCCTC
    AAACTGCTGC TGCTCATGCT GCTGCTCCTC CTCCTCGCCC TCTTCCTCTA CTCTGTGCCC
    GGCTACCTGG TCAAGAAGAT CCTGGGGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18756
    Clone ID Related Accession (Same CDS sequence) XM_005630233.2 , XM_005630234.2 , XM_022404548.1
    Accession Version XM_022404548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGTGCTGC AGAAGGTGGT GGAGGAGAAC CACGTGGAGG TGTCCGACAC GCTGATTGAT 
    GACAACAATG CTATCATCAA GACCAGCCTG TGCGTAGAGG TGCGGTATCA GGCCACAGAC
    GGCACAGTGG GCTCCTGGGA TGATGGGGAC TTCCTGGGAG ACGAATCCCT CCATGAAGAA
    GAGAAGGACA GCCAGGAGAC AGATGGGCTG CTCCCTGGCT CCCGCCCCAG CTCCCGGACT
    CCAGGCGAGA AGAGCTTCCG GAGCAAAGGC AGAGAGAAGA CCAAGGGAGG CAAAGATGGC
    GAGCACAAAG CCGGGAGAAG TGTGTTCTCT GCCATGAAGC TCGGCAAGAA CCGGACCCAC
    AAGGAGGAGC CGCAGAGACA AGATGAACCG GCTGTGCTGG AGATGGAGGA CCTTGACCAC
    TTGGCCATTC GGCTGGGGGA TGGGCTGGAC CCCGATTCTG TGTCTCTAGC CTCTGTCACA
    GCTCTCACCA CCAACGTCTC CAACAAGAGG TCCAAGCCAG ACATTAAGAT GGAGCCATGT
    GCTGGGCGGC CCATGGATTA CCAGGTCAGC ATCACTGTGA TCGAGGCCCG GCAGCTCGTG
    GGCTTGAACA TGGACCCCGT GGTGTGCGTG GAGGTGGGTG ATGACAAGAA GTGCACATCC
    ATGAAGGAGT CCACTAACTG CCCCTACTAC AATGAGTACT TTGTCTTCGA CTTCCACGTC
    TCTCCTGACG TTATGTTCGA CAAGATCATC AAGATCTCGG TGATTCACTC CAAGAACCTG
    CTGCGAAGTG GTACCCTAGT GGGGTCCTTC AAAATGGATG TGGGAACCGT GTACTCCCAG
    CCTGAGCACC AGTTCCACCA CAAGTGGGCC ATCCTGTCAG ACCCCGACGA CATCTCCGCT
    GGGCTGAAGG GCTACGTGAA GTGTGACATC GCTGTGGTGG GCAAGGGGGA CAACATCAAG
    ACACCCCACA AGGCCAACGA GACAGATGAG GATGACATTG AGGGGAACTT GCTGCTGCCC
    GAGGGGGTGC CTGCTGAACG CCAGTGGGCC CGGTTCTACG TGAAAATTTA CCGAGCTGAG
    GGGCTGCCCC GTATGAACAC CAGCCTCATG GCCAATGTGA AGAAGGCTTT CATTGGTGAG
    AACAAGGACC TGGTCGACCC CTATGTGCAA GTCTTCTTTG CTGGCCAGAA GGGCAAGACT
    TCAGTGCAGA AAAGTAGCTA CGAGCCCCTC TGGAATGAGC AGGTCATCTT TACAGACCTG
    TTCCCCCCAC TCTGCAAGCG CATGAAGGTG CAGATCCGAG ACTCAGACAA GGTCAACGAT
    GTGGCCATCG GCACCCACTT CATTGACCTG CGCAAGATTT CCAATGACGG AGACAAAGGC
    TTCCTGCCCA CGCTGGGCCC GGCCTGGGTG AACATGTACG GCTCCACCCG CAACTACACG
    CTGCTGGACG AGCACCAGGA CCTGAATGAG GGCCTGGGCG AGGGCGTGTC GTTCCGCGCC
    CGCCTCATGC TGGGCCTGGC TGTGGAGATC CTGGACACCT CCAACCCCGA GCTCACCAGC
    TCCACGGAGG TACAGGTGGA GCAGGCCACG CCCGTCTCGG AGAGCTGCAC AGGAAAAATG
    GAAGAATTCT TTCTATTTGG AGCCTTCCTA GAGGCCTCAA TGATCGACCG GAAAAACGGA
    GACAAGCCAA TCACCTTTGA GGTCACCATA GGTAACTATG GGAATGAAGT TGATGGCCTG
    TCGAGGCCCC AGCGGGCTCG GCTTCGGAAG GAGCCTGGGG ACGAAGAAGA GGTCGATCTG
    ATCCAGAACT CAAGTGATGA TGAAGCTGAT GAAGGTGGGG ACCTGGCCTC CGTGTCCTCC
    ACCCCTCCCA TGCGGCCCCA GATCACAGAC AGGAACTACT TCCATCTACC CTACCTGGAG
    CGCAAGCCCT GCATCTACAT GAAGAGCTGG TGGCCCGACC AGCGCCGCCG CCTCTACAAT
    GCCAACATTA TGGATCGCAT TGCAGACAAG CTGGAAGAAG GCCTGAATGA CGTGCAGGAG
    ATGATCAAGA CTGAGAAGTC TTACCCTGAG CGTCGCCTCC GGGGTGTCCT GGAGGAGCTC
    AGCTGTGGCT GCTACCGCTT CCTCTCCCTC GCCGACAAGG ACCAGGGCCA CTCATCCCGC
    ACCAGGCTGG ACCGGGAGCG CCTCAAGTCC TGCATGCGGG AGCTGGAAAG CATGGGACAG
    CAGGCCAAGA CCCTGCGGGC TCAGGTGAAG AAGCACACGG TGCGGGACAA GCTGAGGCTA
    TGCCAGAATT TCCTGCAGAA GCTGCGCTTC CTGGCAGATG AGCCCCAGCA CAGCATCCCC
    GATGTCTTCA TCTGGATGAT GAGCAATAAC AAGCGCATTG CCTACGCCCG GGTGCCCTCC
    AAGGACCTGC TCTTCTCCAT CGTTGAGGAG GAGCTGGGAA AGGACTGCGC CAAGGTCAAG
    ACGCTCTTCC TCAAGCTGCC AGGCAAGCGG GGCTTCGGCT CAGCAGGCTG GACGGTGCAG
    GCCAAGCTGG AGCTCTACCT GTGGCTGGGC CTCAGCAAGC AGCGCAAGGA CTTCCTGTGC
    GGGCTGCCCT GTGGCTTCGA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCACTCCTTC
    CCACCCATCA GCCTGGTCTA CACCAAGAAG CAGGCCTTCC AGCTCCGAGC CCACATGTAC
    CAGGCTCGCA GCCTCTTTGC TGCTGACAGC AGTGGCCTCT CAGACCCCTT CGCCCGCGTC
    TTCTTCATCA ACCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG CCCCACCTGG
    GACCAGATGC TGGTATTTGA CAACCTGGAG CTTTATGGTG AAGCCCATGA GTTGAGGGAT
    GATCCCCCTA TCATTGTCAT CGAGATCTAT GACCAGGACG CCATGGGCAA AGCCGACTTC
    ATGGGCCGGA CCTTTGCCAA GCCCCTGGTG AAGATGGCCG ACGAGGCGTA CTGCCCACCC
    CGCTTCCCAC CCCAGCTCGA GTACTACCAA ATCTACAGAG GCAATGCCAC GGCTGGGGAT
    CTGCTGGCTG CCTTCGAGCT GCTGCAGATT GGGCCAGCAG GGAAGGCAGA CCTGCCACCC
    ATCAACGGCC CTGTGGACAT GGACCGAGGT CCCATCATGC CCGTGCCTGT GGGCATCCGG
    CCCGTGCTCA GCAAATACCG AATCGAGGTG CTCTTCTGGG GCCTGAGAGA CCTGAAGCGG
    GTGAACCTGG CCCAGGTGGA CCGGCCGCGG GTGGACATCG AGTGTGCAGG AAAGGGAGTG
    CAGTCGTCCC TGATCCACAA TTACAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG
    TTTGAAGTGG ACCTCCCAGA AAACGAGCTG TTGCACCCGC CCCTGAACAT CCGAGTGGTG
    GACTGCCGGG CCTTCGGCCG CTACACCCTG GTGGGCTCCC ATGCCGTCAG CGCTCTGCGG
    CGATTCATCT ACAGGCCCCC AGACCGCTCA GCCCCCAACT GGAACACCAC AGTCAGGCTC
    CTCCGGGGTG GCCGTGTGCT GTGCAATGGG GGCCCCTCCT CTCACCCCAC AGGGGAAGTC
    GTGGTGAACA TGGAGCCAGA GGTGCCTGTC AAGAAGCTGG AGACCATGGT GAAGCTGGAC
    GCGACTTCTG ATGCTGTCAT CAAGGTGGAT GTGGCTGACG AGGAGAAGGA GAAGAAAAAG
    AAGAAGAAGG GCAACGCAGA AGAGCCAGAG GAAGAGGAGC CAGATGAGAG CATGCTGGAC
    TGGTGGTCCA AGTACTTTGC TTCCATCGAT ACCATGAAGG AGCAACTTCG GCAGCAAGAG
    GTCTCAGGCA CTGACTTGGA GGAGAAAGAG GAGGTGGACA ACACCGAGGG CCTGAAGGGA
    CCGATGAAGG TCAAGGAGAA GGCAAAGGCA GCAAAGGAAG AGAAGAAAAA GAGAACCCAG
    GGCCCCGGCG CCGGCCAGGG GTCTGAAGTC CCTGAGAAGA AGAAACCCAA GATTGATGAG
    CTGAAGGTGT ACTCCAAGGA GCTGGAGTCC GAGTTCGACA ACTTTGAGGA CTGGCTGCAC
    ACTTTCAACC TGCTGCGGGG CAAGACGGGA GATGATGAGG ACGGCTCCAC TGAGGAGGAG
    CGCATCGTGG GCAGGTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAT
    GTGTCCCGGG AAGCCGGCTA CGACCCCACC TATGGCATGT TCCAGGGCAT TCCCAGTAAT
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCAGGG CCACGGACCT GCACCCCGCA
    GACATCAACG GCAAAGCAGA CCCCTACATC GCCATCCGGC TGGGCAAGAC AGACATCCGG
    GACAAGGAGA ACTACATCTC TAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATT
    GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCCG TGTACGACTG GGACCTGGTC
    GGCACCGACG ACCTCATTGG GGAAACCAAG ATTGACCTGG AAAACCGCTT CTACAGCAAG
    CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCATAC ATGGCTACAA TATCTGGCGG
    GACCCCATGA AGCCCAGCCA GATTCTGACC CGTCTCTGCA AGGAGGGCAA AGTGGATGGC
    CCCCACTTTG GGCCCCCCGG GAGAGTGAAA GTGTCCAACC GCGTCTTCAC TGGGCCCTCC
    GAGATCGAGG ACGAGAATGG TCAGAGGAAG CCCACAGAAG AGCACGTGGC ACTGTCTGCC
    CTAAGGCACT GGGAGGACAT CCCCCGTGTG GGCTGCCGCC TCGTGCCTGA ACATGTGGAG
    ACTCGGCCGC TGCTCAACCC TGACAAGCCA GGCATCGAGC AGGGCCGCCT GGAGCTGTGG
    GTGGATATGT TCCCCATGGA CATGCCAGCA CCCGGCACAC CACTGGACAT CTCCCCTCGG
    AAGCCCAAGA AGTACGAGCT CCGGGTCATC GTGTGGAACA CGGACGAGGT GGTCCTGGAG
    GACGATGACT TCTTTACTGG GGAGAAGTCC AGCGACATTT TTGTGCGGGG GTGGCTGAAG
    GGCCAGCAGG AGGACAAGCA GGACACGGAT GTCCACTATC ACTCCCTCAC CGGCGAGGGC
    AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTATCTGG CCGCCGAGGA AAAGATTGTC
    ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACGG AGTACAAGAT CCCCGCGCGG
    CTCACCTTGC AGATCTGGGA CGCCGACCAC TTCTCCGCGG ACGATTTCCT GGGGGCCATC
    GAGCTGGACC TGAACCGGTT CCCCCGGGGC GCGAAGACAG CCAAGCAGTG CACCATGGAG
    ATGGCCACGG GGGAGGTGGA CGTGCCCCTG GTTTCCATCT TCAAGCAGAA GCGCGTCAAA
    GGCTGGTGGC CGCTCCTGGC CCGCGATGAG AATGATGAGT TCGAGCTCAC GGGCAAGGTG
    GAGGCAGAGT TGCATCTCCT GACAGCCGAG GAGGCAGAGA AGAACCCCGT GGGCCTGGCC
    CGCAATGAAC CTGACCCCCT AGAGAAACCC AAGCGGCCAG ACACGAGCTT CATCTGGTTC
    TTGAACCCTC TCAAGTCTGC CCGCTACTTC TTGTGGCACA CATACCGCTG GCTGCTCCTC
    AAACTGCTGC TGCTCATGCT GCTGCTCCTC CTCCTCGCCC TCTTCCTCTA CTCTGTGCCC
    GGCTACCTGG TCAAGAAGAT CCTGGGGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260256.1
    CDS144..5936
    Translation

    Target ORF information:

    RefSeq Version XM_022404548.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGTGCTGC AGAAGGTGGT GGAGGAGAAC CACGTGGAGG TGTCCGACAC GCTGATTGAT 
    GACAACAATG CTATCATCAA GACCAGCCTG TGCGTAGAGG TGCGGTATCA GGCCACAGAC
    GGCACAGTGG GCTCCTGGGA TGATGGGGAC TTCCTGGGAG ACGAATCCCT CCATGAAGAA
    GAGAAGGACA GCCAGGAGAC AGATGGGCTG CTCCCTGGCT CCCGCCCCAG CTCCCGGACT
    CCAGGCGAGA AGAGCTTCCG GAGCAAAGGC AGAGAGAAGA CCAAGGGAGG CAAAGATGGC
    GAGCACAAAG CCGGGAGAAG TGTGTTCTCT GCCATGAAGC TCGGCAAGAA CCGGACCCAC
    AAGGAGGAGC CGCAGAGACA AGATGAACCG GCTGTGCTGG AGATGGAGGA CCTTGACCAC
    TTGGCCATTC GGCTGGGGGA TGGGCTGGAC CCCGATTCTG TGTCTCTAGC CTCTGTCACA
    GCTCTCACCA CCAACGTCTC CAACAAGAGG TCCAAGCCAG ACATTAAGAT GGAGCCATGT
    GCTGGGCGGC CCATGGATTA CCAGGTCAGC ATCACTGTGA TCGAGGCCCG GCAGCTCGTG
    GGCTTGAACA TGGACCCCGT GGTGTGCGTG GAGGTGGGTG ATGACAAGAA GTGCACATCC
    ATGAAGGAGT CCACTAACTG CCCCTACTAC AATGAGTACT TTGTCTTCGA CTTCCACGTC
    TCTCCTGACG TTATGTTCGA CAAGATCATC AAGATCTCGG TGATTCACTC CAAGAACCTG
    CTGCGAAGTG GTACCCTAGT GGGGTCCTTC AAAATGGATG TGGGAACCGT GTACTCCCAG
    CCTGAGCACC AGTTCCACCA CAAGTGGGCC ATCCTGTCAG ACCCCGACGA CATCTCCGCT
    GGGCTGAAGG GCTACGTGAA GTGTGACATC GCTGTGGTGG GCAAGGGGGA CAACATCAAG
    ACACCCCACA AGGCCAACGA GACAGATGAG GATGACATTG AGGGGAACTT GCTGCTGCCC
    GAGGGGGTGC CTGCTGAACG CCAGTGGGCC CGGTTCTACG TGAAAATTTA CCGAGCTGAG
    GGGCTGCCCC GTATGAACAC CAGCCTCATG GCCAATGTGA AGAAGGCTTT CATTGGTGAG
    AACAAGGACC TGGTCGACCC CTATGTGCAA GTCTTCTTTG CTGGCCAGAA GGGCAAGACT
    TCAGTGCAGA AAAGTAGCTA CGAGCCCCTC TGGAATGAGC AGGTCATCTT TACAGACCTG
    TTCCCCCCAC TCTGCAAGCG CATGAAGGTG CAGATCCGAG ACTCAGACAA GGTCAACGAT
    GTGGCCATCG GCACCCACTT CATTGACCTG CGCAAGATTT CCAATGACGG AGACAAAGGC
    TTCCTGCCCA CGCTGGGCCC GGCCTGGGTG AACATGTACG GCTCCACCCG CAACTACACG
    CTGCTGGACG AGCACCAGGA CCTGAATGAG GGCCTGGGCG AGGGCGTGTC GTTCCGCGCC
    CGCCTCATGC TGGGCCTGGC TGTGGAGATC CTGGACACCT CCAACCCCGA GCTCACCAGC
    TCCACGGAGG TACAGGTGGA GCAGGCCACG CCCGTCTCGG AGAGCTGCAC AGGAAAAATG
    GAAGAATTCT TTCTATTTGG AGCCTTCCTA GAGGCCTCAA TGATCGACCG GAAAAACGGA
    GACAAGCCAA TCACCTTTGA GGTCACCATA GGTAACTATG GGAATGAAGT TGATGGCCTG
    TCGAGGCCCC AGCGGGCTCG GCTTCGGAAG GAGCCTGGGG ACGAAGAAGA GGTCGATCTG
    ATCCAGAACT CAAGTGATGA TGAAGCTGAT GAAGGTGGGG ACCTGGCCTC CGTGTCCTCC
    ACCCCTCCCA TGCGGCCCCA GATCACAGAC AGGAACTACT TCCATCTACC CTACCTGGAG
    CGCAAGCCCT GCATCTACAT GAAGAGCTGG TGGCCCGACC AGCGCCGCCG CCTCTACAAT
    GCCAACATTA TGGATCGCAT TGCAGACAAG CTGGAAGAAG GCCTGAATGA CGTGCAGGAG
    ATGATCAAGA CTGAGAAGTC TTACCCTGAG CGTCGCCTCC GGGGTGTCCT GGAGGAGCTC
    AGCTGTGGCT GCTACCGCTT CCTCTCCCTC GCCGACAAGG ACCAGGGCCA CTCATCCCGC
    ACCAGGCTGG ACCGGGAGCG CCTCAAGTCC TGCATGCGGG AGCTGGAAAG CATGGGACAG
    CAGGCCAAGA CCCTGCGGGC TCAGGTGAAG AAGCACACGG TGCGGGACAA GCTGAGGCTA
    TGCCAGAATT TCCTGCAGAA GCTGCGCTTC CTGGCAGATG AGCCCCAGCA CAGCATCCCC
    GATGTCTTCA TCTGGATGAT GAGCAATAAC AAGCGCATTG CCTACGCCCG GGTGCCCTCC
    AAGGACCTGC TCTTCTCCAT CGTTGAGGAG GAGCTGGGAA AGGACTGCGC CAAGGTCAAG
    ACGCTCTTCC TCAAGCTGCC AGGCAAGCGG GGCTTCGGCT CAGCAGGCTG GACGGTGCAG
    GCCAAGCTGG AGCTCTACCT GTGGCTGGGC CTCAGCAAGC AGCGCAAGGA CTTCCTGTGC
    GGGCTGCCCT GTGGCTTCGA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCACTCCTTC
    CCACCCATCA GCCTGGTCTA CACCAAGAAG CAGGCCTTCC AGCTCCGAGC CCACATGTAC
    CAGGCTCGCA GCCTCTTTGC TGCTGACAGC AGTGGCCTCT CAGACCCCTT CGCCCGCGTC
    TTCTTCATCA ACCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG CCCCACCTGG
    GACCAGATGC TGGTATTTGA CAACCTGGAG CTTTATGGTG AAGCCCATGA GTTGAGGGAT
    GATCCCCCTA TCATTGTCAT CGAGATCTAT GACCAGGACG CCATGGGCAA AGCCGACTTC
    ATGGGCCGGA CCTTTGCCAA GCCCCTGGTG AAGATGGCCG ACGAGGCGTA CTGCCCACCC
    CGCTTCCCAC CCCAGCTCGA GTACTACCAA ATCTACAGAG GCAATGCCAC GGCTGGGGAT
    CTGCTGGCTG CCTTCGAGCT GCTGCAGATT GGGCCAGCAG GGAAGGCAGA CCTGCCACCC
    ATCAACGGCC CTGTGGACAT GGACCGAGGT CCCATCATGC CCGTGCCTGT GGGCATCCGG
    CCCGTGCTCA GCAAATACCG AATCGAGGTG CTCTTCTGGG GCCTGAGAGA CCTGAAGCGG
    GTGAACCTGG CCCAGGTGGA CCGGCCGCGG GTGGACATCG AGTGTGCAGG AAAGGGAGTG
    CAGTCGTCCC TGATCCACAA TTACAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG
    TTTGAAGTGG ACCTCCCAGA AAACGAGCTG TTGCACCCGC CCCTGAACAT CCGAGTGGTG
    GACTGCCGGG CCTTCGGCCG CTACACCCTG GTGGGCTCCC ATGCCGTCAG CGCTCTGCGG
    CGATTCATCT ACAGGCCCCC AGACCGCTCA GCCCCCAACT GGAACACCAC AGTCAGGCTC
    CTCCGGGGTG GCCGTGTGCT GTGCAATGGG GGCCCCTCCT CTCACCCCAC AGGGGAAGTC
    GTGGTGAACA TGGAGCCAGA GGTGCCTGTC AAGAAGCTGG AGACCATGGT GAAGCTGGAC
    GCGACTTCTG ATGCTGTCAT CAAGGTGGAT GTGGCTGACG AGGAGAAGGA GAAGAAAAAG
    AAGAAGAAGG GCAACGCAGA AGAGCCAGAG GAAGAGGAGC CAGATGAGAG CATGCTGGAC
    TGGTGGTCCA AGTACTTTGC TTCCATCGAT ACCATGAAGG AGCAACTTCG GCAGCAAGAG
    GTCTCAGGCA CTGACTTGGA GGAGAAAGAG GAGGTGGACA ACACCGAGGG CCTGAAGGGA
    CCGATGAAGG TCAAGGAGAA GGCAAAGGCA GCAAAGGAAG AGAAGAAAAA GAGAACCCAG
    GGCCCCGGCG CCGGCCAGGG GTCTGAAGTC CCTGAGAAGA AGAAACCCAA GATTGATGAG
    CTGAAGGTGT ACTCCAAGGA GCTGGAGTCC GAGTTCGACA ACTTTGAGGA CTGGCTGCAC
    ACTTTCAACC TGCTGCGGGG CAAGACGGGA GATGATGAGG ACGGCTCCAC TGAGGAGGAG
    CGCATCGTGG GCAGGTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAT
    GTGTCCCGGG AAGCCGGCTA CGACCCCACC TATGGCATGT TCCAGGGCAT TCCCAGTAAT
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCAGGG CCACGGACCT GCACCCCGCA
    GACATCAACG GCAAAGCAGA CCCCTACATC GCCATCCGGC TGGGCAAGAC AGACATCCGG
    GACAAGGAGA ACTACATCTC TAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATT
    GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCCG TGTACGACTG GGACCTGGTC
    GGCACCGACG ACCTCATTGG GGAAACCAAG ATTGACCTGG AAAACCGCTT CTACAGCAAG
    CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCATAC ATGGCTACAA TATCTGGCGG
    GACCCCATGA AGCCCAGCCA GATTCTGACC CGTCTCTGCA AGGAGGGCAA AGTGGATGGC
    CCCCACTTTG GGCCCCCCGG GAGAGTGAAA GTGTCCAACC GCGTCTTCAC TGGGCCCTCC
    GAGATCGAGG ACGAGAATGG TCAGAGGAAG CCCACAGAAG AGCACGTGGC ACTGTCTGCC
    CTAAGGCACT GGGAGGACAT CCCCCGTGTG GGCTGCCGCC TCGTGCCTGA ACATGTGGAG
    ACTCGGCCGC TGCTCAACCC TGACAAGCCA GGCATCGAGC AGGGCCGCCT GGAGCTGTGG
    GTGGATATGT TCCCCATGGA CATGCCAGCA CCCGGCACAC CACTGGACAT CTCCCCTCGG
    AAGCCCAAGA AGTACGAGCT CCGGGTCATC GTGTGGAACA CGGACGAGGT GGTCCTGGAG
    GACGATGACT TCTTTACTGG GGAGAAGTCC AGCGACATTT TTGTGCGGGG GTGGCTGAAG
    GGCCAGCAGG AGGACAAGCA GGACACGGAT GTCCACTATC ACTCCCTCAC CGGCGAGGGC
    AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTATCTGG CCGCCGAGGA AAAGATTGTC
    ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACGG AGTACAAGAT CCCCGCGCGG
    CTCACCTTGC AGATCTGGGA CGCCGACCAC TTCTCCGCGG ACGATTTCCT GGGGGCCATC
    GAGCTGGACC TGAACCGGTT CCCCCGGGGC GCGAAGACAG CCAAGCAGTG CACCATGGAG
    ATGGCCACGG GGGAGGTGGA CGTGCCCCTG GTTTCCATCT TCAAGCAGAA GCGCGTCAAA
    GGCTGGTGGC CGCTCCTGGC CCGCGATGAG AATGATGAGT TCGAGCTCAC GGGCAAGGTG
    GAGGCAGAGT TGCATCTCCT GACAGCCGAG GAGGCAGAGA AGAACCCCGT GGGCCTGGCC
    CGCAATGAAC CTGACCCCCT AGAGAAACCC AAGCGGCCAG ACACGAGCTT CATCTGGTTC
    TTGAACCCTC TCAAGTCTGC CCGCTACTTC TTGTGGCACA CATACCGCTG GCTGCTCCTC
    AAACTGCTGC TGCTCATGCT GCTGCTCCTC CTCCTCGCCC TCTTCCTCTA CTCTGTGCCC
    GGCTACCTGG TCAAGAAGAT CCTGGGGGCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294268
    Clone ID Related Accession (Same CDS sequence) XM_005630234.3
    Accession Version XM_005630234.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630234.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGTC
    AGGCTCCTCC GGGGTGGCCG TGTGCTGTGC AATGGGGGCC CCTCCTCTCA CCCCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAAGC GGCCAGACAC GAGCTTCATC
    TGGTTCTTGA ACCCTCTCAA GTCTGCCCGC TACTTCTTGT GGCACACATA CCGCTGGCTG
    CTCCTCAAAC TGCTGCTGCT CATGCTGCTG CTCCTCCTCC TCGCCCTCTT CCTCTACTCT
    GTGCCCGGCT ACCTGGTCAA GAAGATCCTG GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630291.2
    CDS125..6343
    Translation

    Target ORF information:

    RefSeq Version XM_005630234.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630234.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGTC
    AGGCTCCTCC GGGGTGGCCG TGTGCTGTGC AATGGGGGCC CCTCCTCTCA CCCCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAAGC GGCCAGACAC GAGCTTCATC
    TGGTTCTTGA ACCCTCTCAA GTCTGCCCGC TACTTCTTGT GGCACACATA CCGCTGGCTG
    CTCCTCAAAC TGCTGCTGCT CATGCTGCTG CTCCTCCTCC TCGCCCTCTT CCTCTACTCT
    GTGCCCGGCT ACCTGGTCAA GAAGATCCTG GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294269
    Clone ID Related Accession (Same CDS sequence) XM_022404540.1
    Accession Version XM_022404540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGTC
    AGGCTCCTCC GGGGTGGCCG TGTGCTGTGC AATGGGGGCC CCTCCTCTCA CCCCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAACC GGCCTGACAC CGCCTTTGTC
    TGGTTCCTCA ACCCCCTCAA GTCCATCAAG TACCTCATCT GCACGCGATA CAAGTGGCTC
    ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTTATGC TTGCCCTCTT CCTCTACAGC
    CTCCCCGGCT ACATGGTCAA GAAGCTCCTC GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260248.1
    CDS124..6342
    Translation

    Target ORF information:

    RefSeq Version XM_022404540.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGTC
    AGGCTCCTCC GGGGTGGCCG TGTGCTGTGC AATGGGGGCC CCTCCTCTCA CCCCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAACC GGCCTGACAC CGCCTTTGTC
    TGGTTCCTCA ACCCCCTCAA GTCCATCAAG TACCTCATCT GCACGCGATA CAAGTGGCTC
    ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTTATGC TTGCCCTCTT CCTCTACAGC
    CTCCCCGGCT ACATGGTCAA GAAGCTCCTC GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294270
    Clone ID Related Accession (Same CDS sequence) XM_022404541.1
    Accession Version XM_022404541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGTCAGGCT CCTCCGGGGT GGCCGTGTGC TGTGCAATGG GGGCCCCTCC
    TCTCACCCCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAAGCGGCCA
    GACACGAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CTTGTGGCAC
    ACATACCGCT GGCTGCTCCT CAAACTGCTG CTGCTCATGC TGCTGCTCCT CCTCCTCGCC
    CTCTTCCTCT ACTCTGTGCC CGGCTACCTG GTCAAGAAGA TCCTGGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260249.1
    CDS124..6297
    Translation

    Target ORF information:

    RefSeq Version XM_022404541.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGA GCCGGGAGAA GTGTGTTCTC TGCCATGAAG
    CTCGGCAAGA ACCGGACCCA CAAGGAGGAG CCGCAGAGAC AAGATGAACC GGCTGTGCTG
    GAGATGGAGG ACCTTGACCA CTTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCCGATTCT
    GTGTCTCTAG CCTCTGTCAC AGCTCTCACC ACCAACGTCT CCAACAAGAG GTCCAAGCCA
    GACATTAAGA TGGAGCCATG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACTGTG
    ATCGAGGCCC GGCAGCTCGT GGGCTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGT
    GATGACAAGA AGTGCACATC CATGAAGGAG TCCACTAACT GCCCCTACTA CAATGAGTAC
    TTTGTCTTCG ACTTCCACGT CTCTCCTGAC GTTATGTTCG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGAAGT GGTACCCTAG TGGGGTCCTT CAAAATGGAT
    GTGGGAACCG TGTACTCCCA GCCTGAGCAC CAGTTCCACC ACAAGTGGGC CATCCTGTCA
    GACCCCGACG ACATCTCCGC TGGGCTGAAG GGCTACGTGA AGTGTGACAT CGCTGTGGTG
    GGCAAGGGGG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT
    GAGGGGAACT TGCTGCTGCC CGAGGGGGTG CCTGCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAAATTT ACCGAGCTGA GGGGCTGCCC CGTATGAACA CCAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGTAGCT ACGAGCCCCT CTGGAATGAG
    CAGGTCATCT TTACAGACCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA
    GACTCAGACA AGGTCAACGA TGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAATGACG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CGGCCTGGGT GAACATGTAC
    GGCTCCACCC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGC
    GAGGGCGTGT CGTTCCGCGC CCGCCTCATG CTGGGCCTGG CTGTGGAGAT CCTGGACACC
    TCCAACCCCG AGCTCACCAG CTCCACGGAG GTACAGGTGG AGCAGGCCAC GCCCGTCTCG
    GAGAGCTGCA CAGGAAAAAT GGAAGAATTC TTTCTATTTG GAGCCTTCCT AGAGGCCTCA
    ATGATCGACC GGAAAAACGG AGACAAGCCA ATCACCTTTG AGGTCACCAT AGGTAACTAT
    GGGAATGAAG TTGATGGCCT GTCGAGGCCC CAGCGGGCTC GGCTTCGGAA GGAGCCTGGG
    GACGAAGAAG AGGTCGATCT GATCCAGAAC TCAAGTGATG ATGAAGCTGA TGAAGGTGGG
    GACCTGGCCT CCGTGTCCTC CACCCCTCCC ATGCGGCCCC AGATCACAGA CAGGAACTAC
    TTCCATCTAC CCTACCTGGA GCGCAAGCCC TGCATCTACA TGAAGAGCTG GTGGCCCGAC
    CAGCGCCGCC GCCTCTACAA TGCCAACATT ATGGATCGCA TTGCAGACAA GCTGGAAGAA
    GGCCTGAATG ACGTGCAGGA GATGATCAAG ACTGAGAAGT CTTACCCTGA GCGTCGCCTC
    CGGGGTGTCC TGGAGGAGCT CAGCTGTGGC TGCTACCGCT TCCTCTCCCT CGCCGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GCCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGACA GCAGGCCAAG ACCCTGCGGG CTCAGGTGAA GAAGCACACG
    GTGCGGGACA AGCTGAGGCT ATGCCAGAAT TTCCTGCAGA AGCTGCGCTT CCTGGCAGAT
    GAGCCCCAGC ACAGCATCCC CGATGTCTTC ATCTGGATGA TGAGCAATAA CAAGCGCATT
    GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTTGAGGA GGAGCTGGGA
    AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTCAAGCTGC CAGGCAAGCG GGGCTTCGGC
    TCAGCAGGCT GGACGGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGCAAG
    CAGCGCAAGG ACTTCCTGTG CGGGCTGCCC TGTGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TGCACTCCTT CCCACCCATC AGCCTGGTCT ACACCAAGAA GCAGGCCTTC
    CAGCTCCGAG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CAGTGGCCTC
    TCAGACCCCT TCGCCCGCGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTGCTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTATTTG ACAACCTGGA GCTTTATGGT
    GAAGCCCATG AGTTGAGGGA TGATCCCCCT ATCATTGTCA TCGAGATCTA TGACCAGGAC
    GCCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCCCAGCTCG AGTACTACCA AATCTACAGA
    GGCAATGCCA CGGCTGGGGA TCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCAGCA
    GGGAAGGCAG ACCTGCCACC CATCAACGGC CCTGTGGACA TGGACCGAGG TCCCATCATG
    CCCGTGCCTG TGGGCATCCG GCCCGTGCTC AGCAAATACC GAATCGAGGT GCTCTTCTGG
    GGCCTGAGAG ACCTGAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCGCG GGTGGACATC
    GAGTGTGCAG GAAAGGGAGT GCAGTCGTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AAAACGAGCT GTTGCACCCG
    CCCCTGAACA TCCGAGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTGGGCTCC
    CATGCCGTCA GCGCTCTGCG GCGATTCATC TACAGGCCCC CAGACCGCTC AGCCCCCAAC
    TGGAACACCA CAGTCAGGCT CCTCCGGGGT GGCCGTGTGC TGTGCAATGG GGGCCCCTCC
    TCTCACCCCA CAGGGGAAGT CGTGGTGAAC ATGGAGCCAG AGGTGCCTGT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCT GATGCTGTCA TCAAGGTGGA TGTGGCTGAC
    GAGGAGAAGG AGAAGAAAAA GAAGAAGAAG GGCAACGCAG AAGAGCCAGA GGAAGAGGAG
    CCAGATGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CTTCCATCGA TACCATGAAG
    GAGCAACTTC GGCAGCAAGA GGTCTCAGGC ACTGACTTGG AGGAGAAAGA GGAGGTGGAC
    AACACCGAGG GCCTGAAGGG ACCGATGAAG GTCAAGGAGA AGGCAAAGGC AGCAAAGGAA
    GAGAAGAAAA AGAGAACCCA GGGCCCCGGC GCCGGCCAGG GGTCTGAAGT CCCTGAGAAG
    AAGAAACCCA AGATTGATGA GCTGAAGGTG TACTCCAAGG AGCTGGAGTC CGAGTTCGAC
    AACTTTGAGG ACTGGCTGCA CACTTTCAAC CTGCTGCGGG GCAAGACGGG AGATGATGAG
    GACGGCTCCA CTGAGGAGGA GCGCATCGTG GGCAGGTTCA AGGGCTCCCT CTGCGTGTAC
    AAAGTGCCAC TCCCAGAGGA TGTGTCCCGG GAAGCCGGCT ACGACCCCAC CTATGGCATG
    TTCCAGGGCA TTCCCAGTAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCAGG
    GCCACGGACC TGCACCCCGC AGACATCAAC GGCAAAGCAG ACCCCTACAT CGCCATCCGG
    CTGGGCAAGA CAGACATCCG GGACAAGGAG AACTACATCT CTAAGCAGCT CAACCCTGTC
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCC
    GTGTACGACT GGGACCTGGT CGGCACCGAC GACCTCATTG GGGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCATA
    CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATTCTGAC CCGTCTCTGC
    AAGGAGGGCA AAGTGGATGG CCCCCACTTT GGGCCCCCCG GGAGAGTGAA AGTGTCCAAC
    CGCGTCTTCA CTGGGCCCTC CGAGATCGAG GACGAGAATG GTCAGAGGAA GCCCACAGAA
    GAGCACGTGG CACTGTCTGC CCTAAGGCAC TGGGAGGACA TCCCCCGTGT GGGCTGCCGC
    CTCGTGCCTG AACATGTGGA GACTCGGCCG CTGCTCAACC CTGACAAGCC AGGCATCGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGATATG TTCCCCATGG ACATGCCAGC ACCCGGCACA
    CCACTGGACA TCTCCCCTCG GAAGCCCAAG AAGTACGAGC TCCGGGTCAT CGTGTGGAAC
    ACGGACGAGG TGGTCCTGGA GGACGATGAC TTCTTTACTG GGGAGAAGTC CAGCGACATT
    TTTGTGCGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACGGA TGTCCACTAT
    CACTCCCTCA CCGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTATCTG
    GCCGCCGAGG AAAAGATTGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACG
    GAGTACAAGA TCCCCGCGCG GCTCACCTTG CAGATCTGGG ACGCCGACCA CTTCTCCGCG
    GACGATTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCCCGGGG CGCGAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACG GGGGAGGTGG ACGTGCCCCT GGTTTCCATC
    TTCAAGCAGA AGCGCGTCAA AGGCTGGTGG CCGCTCCTGG CCCGCGATGA GAATGATGAG
    TTCGAGCTCA CGGGCAAGGT GGAGGCAGAG TTGCATCTCC TGACAGCCGA GGAGGCAGAG
    AAGAACCCCG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAAGCGGCCA
    GACACGAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CTTGTGGCAC
    ACATACCGCT GGCTGCTCCT CAAACTGCTG CTGCTCATGC TGCTGCTCCT CCTCCTCGCC
    CTCTTCCTCT ACTCTGTGCC CGGCTACCTG GTCAAGAAGA TCCTGGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294271
    Clone ID Related Accession (Same CDS sequence) XM_022404542.1
    Accession Version XM_022404542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAAGC GGCCAGACAC GAGCTTCATC
    TGGTTCTTGA ACCCTCTCAA GTCTGCCCGC TACTTCTTGT GGCACACATA CCGCTGGCTG
    CTCCTCAAAC TGCTGCTGCT CATGCTGCTG CTCCTCCTCC TCGCCCTCTT CCTCTACTCT
    GTGCCCGGCT ACCTGGTCAA GAAGATCCTG GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260250.1
    CDS125..6283
    Translation

    Target ORF information:

    RefSeq Version XM_022404542.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAAGC GGCCAGACAC GAGCTTCATC
    TGGTTCTTGA ACCCTCTCAA GTCTGCCCGC TACTTCTTGT GGCACACATA CCGCTGGCTG
    CTCCTCAAAC TGCTGCTGCT CATGCTGCTG CTCCTCCTCC TCGCCCTCTT CCTCTACTCT
    GTGCCCGGCT ACCTGGTCAA GAAGATCCTG GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294272
    Clone ID Related Accession (Same CDS sequence) XM_022404543.1
    Accession Version XM_022404543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAACC GGCCTGACAC CGCCTTTGTC
    TGGTTCCTCA ACCCCCTCAA GTCCATCAAG TACCTCATCT GCACGCGATA CAAGTGGCTC
    ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTTATGC TTGCCCTCTT CCTCTACAGC
    CTCCCCGGCT ACATGGTCAA GAAGCTCCTC GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260251.1
    CDS125..6283
    Translation

    Target ORF information:

    RefSeq Version XM_022404543.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAACC GGCCTGACAC CGCCTTTGTC
    TGGTTCCTCA ACCCCCTCAA GTCCATCAAG TACCTCATCT GCACGCGATA CAAGTGGCTC
    ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTTATGC TTGCCCTCTT CCTCTACAGC
    CTCCCCGGCT ACATGGTCAA GAAGCTCCTC GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294273
    Clone ID Related Accession (Same CDS sequence) XM_022404544.1
    Accession Version XM_022404544.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GATTGGGCCA
    GCAGGGAAGG CAGACCTGCC ACCCATCAAC GGCCCTGTGG ACATGGACCG AGGTCCCATC
    ATGCCCGTGC CTGTGGGCAT CCGGCCCGTG CTCAGCAAAT ACCGAATCGA GGTGCTCTTC
    TGGGGCCTGA GAGACCTGAA GCGGGTGAAC CTGGCCCAGG TGGACCGGCC GCGGGTGGAC
    ATCGAGTGTG CAGGAAAGGG AGTGCAGTCG TCCCTGATCC ACAATTACAA GAAGAACCCC
    AACTTCAACA CCCTCGTCAA GTGGTTTGAA GTGGACCTCC CAGAAAACGA GCTGTTGCAC
    CCGCCCCTGA ACATCCGAGT GGTGGACTGC CGGGCCTTCG GCCGCTACAC CCTGGTGGGC
    TCCCATGCCG TCAGCGCTCT GCGGCGATTC ATCTACAGGC CCCCAGACCG CTCAGCCCCC
    AACTGGAACA CCACAGTCAG GCTCCTCCGG GGTGGCCGTG TGCTGTGCAA TGGGGGCCCC
    TCCTCTCACC CCACAGGGGA AGTCGTGGTG AACATGGAGC CAGAGGTGCC TGTCAAGAAG
    CTGGAGACCA TGGTGAAGCT GGACGCGACT TCTGATGCTG TCATCAAGGT GGATGTGGCT
    GACGAGGAGA AGGAGAAGAA AAAGAAGAAG AAGGGCAACG CAGAAGAGCC AGAGGAAGAG
    GAGCCAGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCTTCCAT CGATACCATG
    AAGGAGCAAC TTCGGCAGCA AGAGGTCTCA GGCACTGACT TGGAGGAGAA AGAGGAGGTG
    GACAACACCG AGGGCCTGAA GGGACCGATG AAGGTCAAGG AGAAGGCAAA GGCAGCAAAG
    GAAGAGAAGA AAAAGAGAAC CCAGGGCCCC GGCGCCGGCC AGGGGTCTGA AGTCCCTGAG
    AAGAAGAAAC CCAAGATTGA TGAGCTGAAG GTGTACTCCA AGGAGCTGGA GTCCGAGTTC
    GACAACTTTG AGGACTGGCT GCACACTTTC AACCTGCTGC GGGGCAAGAC GGGAGATGAT
    GAGGACGGCT CCACTGAGGA GGAGCGCATC GTGGGCAGGT TCAAGGGCTC CCTCTGCGTG
    TACAAAGTGC CACTCCCAGA GGATGTGTCC CGGGAAGCCG GCTACGACCC CACCTATGGC
    ATGTTCCAGG GCATTCCCAG TAATGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC
    AGGGCCACGG ACCTGCACCC CGCAGACATC AACGGCAAAG CAGACCCCTA CATCGCCATC
    CGGCTGGGCA AGACAGACAT CCGGGACAAG GAGAACTACA TCTCTAAGCA GCTCAACCCT
    GTCTTTGGGA AGTCCTTTGA CATTGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACGGTG
    GCCGTGTACG ACTGGGACCT GGTCGGCACC GACGACCTCA TTGGGGAAAC CAAGATTGAC
    CTGGAAAACC GCTTCTACAG CAAGCACCGC GCCACCTGCG GCATCGCCCA GACCTACTCC
    ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGTCTC
    TGCAAGGAGG GCAAAGTGGA TGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAAGTGTCC
    AACCGCGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCCACA
    GAAGAGCACG TGGCACTGTC TGCCCTAAGG CACTGGGAGG ACATCCCCCG TGTGGGCTGC
    CGCCTCGTGC CTGAACATGT GGAGACTCGG CCGCTGCTCA ACCCTGACAA GCCAGGCATC
    GAGCAGGGCC GCCTGGAGCT GTGGGTGGAT ATGTTCCCCA TGGACATGCC AGCACCCGGC
    ACACCACTGG ACATCTCCCC TCGGAAGCCC AAGAAGTACG AGCTCCGGGT CATCGTGTGG
    AACACGGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC
    ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGATGTCCAC
    TATCACTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAT
    CTGGCCGCCG AGGAAAAGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG
    ACGGAGTACA AGATCCCCGC GCGGCTCACC TTGCAGATCT GGGACGCCGA CCACTTCTCC
    GCGGACGATT TCCTGGGGGC CATCGAGCTG GACCTGAACC GGTTCCCCCG GGGCGCGAAG
    ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC
    ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCGCTCC TGGCCCGCGA TGAGAATGAT
    GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAGTTGCATC TCCTGACAGC CGAGGAGGCA
    GAGAAGAACC CCGTGGGCCT GGCCCGCAAT GAACCTGACC CCCTAGAGAA ACCCAAGCGG
    CCAGACACGA GCTTCATCTG GTTCTTGAAC CCTCTCAAGT CTGCCCGCTA CTTCTTGTGG
    CACACATACC GCTGGCTGCT CCTCAAACTG CTGCTGCTCA TGCTGCTGCT CCTCCTCCTC
    GCCCTCTTCC TCTACTCTGT GCCCGGCTAC CTGGTCAAGA AGATCCTGGG GGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260252.1
    CDS126..6182
    Translation

    Target ORF information:

    RefSeq Version XM_022404544.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404544.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGAAGGAAA GAATTAATAG GAAAATCCTA AGCCACCTTT TCTTACACAC CCAGCTTGAC 
    ATCAGGAGCA TCTCAAAATC CAGTTCGTGC CCTCCATGCC TGGGGCCACC CGTAGGAGAA
    AGACGAGGAT CTTACACCCT GGAAGAAGGG GTTGGAGTGG AAATCTCAGA CACTTTGAGA
    GAAAGGCCAA AGAATGGAAG AGACAGTCAG ACTTGCATCT TGGATGTTTG GAGCTTGATA
    ACAGCCAGTG AAAGGAAAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GATTGGGCCA
    GCAGGGAAGG CAGACCTGCC ACCCATCAAC GGCCCTGTGG ACATGGACCG AGGTCCCATC
    ATGCCCGTGC CTGTGGGCAT CCGGCCCGTG CTCAGCAAAT ACCGAATCGA GGTGCTCTTC
    TGGGGCCTGA GAGACCTGAA GCGGGTGAAC CTGGCCCAGG TGGACCGGCC GCGGGTGGAC
    ATCGAGTGTG CAGGAAAGGG AGTGCAGTCG TCCCTGATCC ACAATTACAA GAAGAACCCC
    AACTTCAACA CCCTCGTCAA GTGGTTTGAA GTGGACCTCC CAGAAAACGA GCTGTTGCAC
    CCGCCCCTGA ACATCCGAGT GGTGGACTGC CGGGCCTTCG GCCGCTACAC CCTGGTGGGC
    TCCCATGCCG TCAGCGCTCT GCGGCGATTC ATCTACAGGC CCCCAGACCG CTCAGCCCCC
    AACTGGAACA CCACAGTCAG GCTCCTCCGG GGTGGCCGTG TGCTGTGCAA TGGGGGCCCC
    TCCTCTCACC CCACAGGGGA AGTCGTGGTG AACATGGAGC CAGAGGTGCC TGTCAAGAAG
    CTGGAGACCA TGGTGAAGCT GGACGCGACT TCTGATGCTG TCATCAAGGT GGATGTGGCT
    GACGAGGAGA AGGAGAAGAA AAAGAAGAAG AAGGGCAACG CAGAAGAGCC AGAGGAAGAG
    GAGCCAGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCTTCCAT CGATACCATG
    AAGGAGCAAC TTCGGCAGCA AGAGGTCTCA GGCACTGACT TGGAGGAGAA AGAGGAGGTG
    GACAACACCG AGGGCCTGAA GGGACCGATG AAGGTCAAGG AGAAGGCAAA GGCAGCAAAG
    GAAGAGAAGA AAAAGAGAAC CCAGGGCCCC GGCGCCGGCC AGGGGTCTGA AGTCCCTGAG
    AAGAAGAAAC CCAAGATTGA TGAGCTGAAG GTGTACTCCA AGGAGCTGGA GTCCGAGTTC
    GACAACTTTG AGGACTGGCT GCACACTTTC AACCTGCTGC GGGGCAAGAC GGGAGATGAT
    GAGGACGGCT CCACTGAGGA GGAGCGCATC GTGGGCAGGT TCAAGGGCTC CCTCTGCGTG
    TACAAAGTGC CACTCCCAGA GGATGTGTCC CGGGAAGCCG GCTACGACCC CACCTATGGC
    ATGTTCCAGG GCATTCCCAG TAATGACCCC ATCAATGTGC TGGTCCGAGT CTATGTGGTC
    AGGGCCACGG ACCTGCACCC CGCAGACATC AACGGCAAAG CAGACCCCTA CATCGCCATC
    CGGCTGGGCA AGACAGACAT CCGGGACAAG GAGAACTACA TCTCTAAGCA GCTCAACCCT
    GTCTTTGGGA AGTCCTTTGA CATTGAGGCC TCCTTCCCCA TGGAATCCAT GCTGACGGTG
    GCCGTGTACG ACTGGGACCT GGTCGGCACC GACGACCTCA TTGGGGAAAC CAAGATTGAC
    CTGGAAAACC GCTTCTACAG CAAGCACCGC GCCACCTGCG GCATCGCCCA GACCTACTCC
    ATACATGGCT ACAATATCTG GCGGGACCCC ATGAAGCCCA GCCAGATTCT GACCCGTCTC
    TGCAAGGAGG GCAAAGTGGA TGGCCCCCAC TTTGGGCCCC CCGGGAGAGT GAAAGTGTCC
    AACCGCGTCT TCACTGGGCC CTCCGAGATC GAGGACGAGA ATGGTCAGAG GAAGCCCACA
    GAAGAGCACG TGGCACTGTC TGCCCTAAGG CACTGGGAGG ACATCCCCCG TGTGGGCTGC
    CGCCTCGTGC CTGAACATGT GGAGACTCGG CCGCTGCTCA ACCCTGACAA GCCAGGCATC
    GAGCAGGGCC GCCTGGAGCT GTGGGTGGAT ATGTTCCCCA TGGACATGCC AGCACCCGGC
    ACACCACTGG ACATCTCCCC TCGGAAGCCC AAGAAGTACG AGCTCCGGGT CATCGTGTGG
    AACACGGACG AGGTGGTCCT GGAGGACGAT GACTTCTTTA CTGGGGAGAA GTCCAGCGAC
    ATTTTTGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC GGATGTCCAC
    TATCACTCCC TCACCGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAT
    CTGGCCGCCG AGGAAAAGAT TGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG
    ACGGAGTACA AGATCCCCGC GCGGCTCACC TTGCAGATCT GGGACGCCGA CCACTTCTCC
    GCGGACGATT TCCTGGGGGC CATCGAGCTG GACCTGAACC GGTTCCCCCG GGGCGCGAAG
    ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACGGGGGAGG TGGACGTGCC CCTGGTTTCC
    ATCTTCAAGC AGAAGCGCGT CAAAGGCTGG TGGCCGCTCC TGGCCCGCGA TGAGAATGAT
    GAGTTCGAGC TCACGGGCAA GGTGGAGGCA GAGTTGCATC TCCTGACAGC CGAGGAGGCA
    GAGAAGAACC CCGTGGGCCT GGCCCGCAAT GAACCTGACC CCCTAGAGAA ACCCAAGCGG
    CCAGACACGA GCTTCATCTG GTTCTTGAAC CCTCTCAAGT CTGCCCGCTA CTTCTTGTGG
    CACACATACC GCTGGCTGCT CCTCAAACTG CTGCTGCTCA TGCTGCTGCT CCTCCTCCTC
    GCCCTCTTCC TCTACTCTGT GCCCGGCTAC CTGGTCAAGA AGATCCTGGG GGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40800
    Clone ID Related Accession (Same CDS sequence) XM_014120469.1
    Accession Version XM_014120469.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6039bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product otoferlin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGTC
    AGGCTCCTCC GGGGTGGCCG TGTGCTGTGC AATGGGGGCC CCTCCTCTCA CCCCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAACC GGCCTGACAC CGCCTTTGTC
    TGGTTCCTCA ACCCCCTCAA GTCCATCAAG TACCTCATCT GCACGCGATA CAAGTGGCTC
    ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTTATGC TTGCCCTCTT CCTCTACAGC
    CTCCCCGGCT ACATGGTCAA GAAGCTCCTC GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975944.1
    CDS368..6406
    Translation

    Target ORF information:

    RefSeq Version XM_014120469.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120469.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGTC
    AGGCTCCTCC GGGGTGGCCG TGTGCTGTGC AATGGGGGCC CCTCCTCTCA CCCCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAACC GGCCTGACAC CGCCTTTGTC
    TGGTTCCTCA ACCCCCTCAA GTCCATCAAG TACCTCATCT GCACGCGATA CAAGTGGCTC
    ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTTATGC TTGCCCTCTT CCTCTACAGC
    CTCCCCGGCT ACATGGTCAA GAAGCTCCTC GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18750
    Clone ID Related Accession (Same CDS sequence) XM_022404545.1 , XM_005630228.2
    Accession Version XM_022404545.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6039bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product otoferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGTC
    AGGCTCCTCC GGGGTGGCCG TGTGCTGTGC AATGGGGGCC CCTCCTCTCA CCCCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAAGC GGCCAGACAC GAGCTTCATC
    TGGTTCTTGA ACCCTCTCAA GTCTGCCCGC TACTTCTTGT GGCACACATA CCGCTGGCTG
    CTCCTCAAAC TGCTGCTGCT CATGCTGCTG CTCCTCCTCC TCGCCCTCTT CCTCTACTCT
    GTGCCCGGCT ACCTGGTCAA GAAGATCCTG GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260253.1
    CDS371..6409
    Translation

    Target ORF information:

    RefSeq Version XM_022404545.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404545.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGTC
    AGGCTCCTCC GGGGTGGCCG TGTGCTGTGC AATGGGGGCC CCTCCTCTCA CCCCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAAGC GGCCAGACAC GAGCTTCATC
    TGGTTCTTGA ACCCTCTCAA GTCTGCCCGC TACTTCTTGT GGCACACATA CCGCTGGCTG
    CTCCTCAAAC TGCTGCTGCT CATGCTGCTG CTCCTCCTCC TCGCCCTCTT CCTCTACTCT
    GTGCCCGGCT ACCTGGTCAA GAAGATCCTG GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe18750
    Clone ID Related Accession (Same CDS sequence) XM_022404545.1 , XM_005630228.2
    Accession Version XM_005630228.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6039bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product otoferlin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630228.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGTC
    AGGCTCCTCC GGGGTGGCCG TGTGCTGTGC AATGGGGGCC CCTCCTCTCA CCCCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAAGC GGCCAGACAC GAGCTTCATC
    TGGTTCTTGA ACCCTCTCAA GTCTGCCCGC TACTTCTTGT GGCACACATA CCGCTGGCTG
    CTCCTCAAAC TGCTGCTGCT CATGCTGCTG CTCCTCCTCC TCGCCCTCTT CCTCTACTCT
    GTGCCCGGCT ACCTGGTCAA GAAGATCCTG GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630285.1
    CDS369..6407
    Translation

    Target ORF information:

    RefSeq Version XM_005630228.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris otoferlin (OTOF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630228.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC TTGTCCACCT CAAGACGGTC TCGGAGCTGC GGGGCAGGGG CGACAGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAAGCCTTC TACTCCCGAG TGCTGGAGAA CTGCGAGGAT
    GTGGCGGACT TCGACGAGAC CTTCCGGTGG CCAGTGGCCA GCAACATCGA CAGCAATGAG
    ATACTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT AATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGTC CGACACGCTG
    ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TAGAGGTGCG GTATCAGGCC
    ACAGACGGCA CAGTGGGCTC CTGGGATGAT GGGGACTTCC TGGGAGACGA ATCCCTCCAT
    GAAGAAGAGA AGGACAGCCA GGAGACAGAT GGGCTGCTCC CTGGCTCCCG CCCCAGCTCC
    CGGACTCCAG GCGAGAAGAG CTTCCGGAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAAA
    GATGGCGAGC ACAAAGCCGG GAGAAGTGTG TTCTCTGCCA TGAAGCTCGG CAAGAACCGG
    ACCCACAAGG AGGAGCCGCA GAGACAAGAT GAACCGGCTG TGCTGGAGAT GGAGGACCTT
    GACCACTTGG CCATTCGGCT GGGGGATGGG CTGGACCCCG ATTCTGTGTC TCTAGCCTCT
    GTCACAGCTC TCACCACCAA CGTCTCCAAC AAGAGGTCCA AGCCAGACAT TAAGATGGAG
    CCATGTGCTG GGCGGCCCAT GGATTACCAG GTCAGCATCA CTGTGATCGA GGCCCGGCAG
    CTCGTGGGCT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGTGATGA CAAGAAGTGC
    ACATCCATGA AGGAGTCCAC TAACTGCCCC TACTACAATG AGTACTTTGT CTTCGACTTC
    CACGTCTCTC CTGACGTTAT GTTCGACAAG ATCATCAAGA TCTCGGTGAT TCACTCCAAG
    AACCTGCTGC GAAGTGGTAC CCTAGTGGGG TCCTTCAAAA TGGATGTGGG AACCGTGTAC
    TCCCAGCCTG AGCACCAGTT CCACCACAAG TGGGCCATCC TGTCAGACCC CGACGACATC
    TCCGCTGGGC TGAAGGGCTA CGTGAAGTGT GACATCGCTG TGGTGGGCAA GGGGGACAAC
    ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAGGG GAACTTGCTG
    CTGCCCGAGG GGGTGCCTGC TGAACGCCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA
    GCTGAGGGGC TGCCCCGTAT GAACACCAGC CTCATGGCCA ATGTGAAGAA GGCTTTCATT
    GGTGAGAACA AGGACCTGGT CGACCCCTAT GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC
    AAGACTTCAG TGCAGAAAAG TAGCTACGAG CCCCTCTGGA ATGAGCAGGT CATCTTTACA
    GACCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC AGACAAGGTC
    AACGATGTGG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA TGACGGAGAC
    AAAGGCTTCC TGCCCACGCT GGGCCCGGCC TGGGTGAACA TGTACGGCTC CACCCGCAAC
    TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGCGAGGG CGTGTCGTTC
    CGCGCCCGCC TCATGCTGGG CCTGGCTGTG GAGATCCTGG ACACCTCCAA CCCCGAGCTC
    ACCAGCTCCA CGGAGGTACA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA
    AAAATGGAAG AATTCTTTCT ATTTGGAGCC TTCCTAGAGG CCTCAATGAT CGACCGGAAA
    AACGGAGACA AGCCAATCAC CTTTGAGGTC ACCATAGGTA ACTATGGGAA TGAAGTTGAT
    GGCCTGTCGA GGCCCCAGCG GGCTCGGCTT CGGAAGGAGC CTGGGGACGA AGAAGAGGTC
    GATCTGATCC AGAACTCAAG TGATGATGAA GCTGATGAAG GTGGGGACCT GGCCTCCGTG
    TCCTCCACCC CTCCCATGCG GCCCCAGATC ACAGACAGGA ACTACTTCCA TCTACCCTAC
    CTGGAGCGCA AGCCCTGCAT CTACATGAAG AGCTGGTGGC CCGACCAGCG CCGCCGCCTC
    TACAATGCCA ACATTATGGA TCGCATTGCA GACAAGCTGG AAGAAGGCCT GAATGACGTG
    CAGGAGATGA TCAAGACTGA GAAGTCTTAC CCTGAGCGTC GCCTCCGGGG TGTCCTGGAG
    GAGCTCAGCT GTGGCTGCTA CCGCTTCCTC TCCCTCGCCG ACAAGGACCA GGGCCACTCA
    TCCCGCACCA GGCTGGACCG GGAGCGCCTC AAGTCCTGCA TGCGGGAGCT GGAAAGCATG
    GGACAGCAGG CCAAGACCCT GCGGGCTCAG GTGAAGAAGC ACACGGTGCG GGACAAGCTG
    AGGCTATGCC AGAATTTCCT GCAGAAGCTG CGCTTCCTGG CAGATGAGCC CCAGCACAGC
    ATCCCCGATG TCTTCATCTG GATGATGAGC AATAACAAGC GCATTGCCTA CGCCCGGGTG
    CCCTCCAAGG ACCTGCTCTT CTCCATCGTT GAGGAGGAGC TGGGAAAGGA CTGCGCCAAG
    GTCAAGACGC TCTTCCTCAA GCTGCCAGGC AAGCGGGGCT TCGGCTCAGC AGGCTGGACG
    GTGCAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GCAAGCAGCG CAAGGACTTC
    CTGTGCGGGC TGCCCTGTGG CTTCGAGGAG GTCAAGGCAG CCCAGGGCCT GGGCCTGCAC
    TCCTTCCCAC CCATCAGCCT GGTCTACACC AAGAAGCAGG CCTTCCAGCT CCGAGCCCAC
    ATGTACCAGG CTCGCAGCCT CTTTGCTGCT GACAGCAGTG GCCTCTCAGA CCCCTTCGCC
    CGCGTCTTCT TCATCAACCA GAGTCAGTGC ACAGAGGTGC TGAATGAGAC CCTGTGCCCC
    ACCTGGGACC AGATGCTGGT ATTTGACAAC CTGGAGCTTT ATGGTGAAGC CCATGAGTTG
    AGGGATGATC CCCCTATCAT TGTCATCGAG ATCTATGACC AGGACGCCAT GGGCAAAGCC
    GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCCGACGA GGCGTACTGC
    CCACCCCGCT TCCCACCCCA GCTCGAGTAC TACCAAATCT ACAGAGGCAA TGCCACGGCT
    GGGGATCTGC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CAGCAGGGAA GGCAGACCTG
    CCACCCATCA ACGGCCCTGT GGACATGGAC CGAGGTCCCA TCATGCCCGT GCCTGTGGGC
    ATCCGGCCCG TGCTCAGCAA ATACCGAATC GAGGTGCTCT TCTGGGGCCT GAGAGACCTG
    AAGCGGGTGA ACCTGGCCCA GGTGGACCGG CCGCGGGTGG ACATCGAGTG TGCAGGAAAG
    GGAGTGCAGT CGTCCCTGAT CCACAATTAC AAGAAGAACC CCAACTTCAA CACCCTCGTC
    AAGTGGTTTG AAGTGGACCT CCCAGAAAAC GAGCTGTTGC ACCCGCCCCT GAACATCCGA
    GTGGTGGACT GCCGGGCCTT CGGCCGCTAC ACCCTGGTGG GCTCCCATGC CGTCAGCGCT
    CTGCGGCGAT TCATCTACAG GCCCCCAGAC CGCTCAGCCC CCAACTGGAA CACCACAGTC
    AGGCTCCTCC GGGGTGGCCG TGTGCTGTGC AATGGGGGCC CCTCCTCTCA CCCCACAGGG
    GAAGTCGTGG TGAACATGGA GCCAGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG
    CTGGACGCGA CTTCTGATGC TGTCATCAAG GTGGATGTGG CTGACGAGGA GAAGGAGAAG
    AAAAAGAAGA AGAAGGGCAA CGCAGAAGAG CCAGAGGAAG AGGAGCCAGA TGAGAGCATG
    CTGGACTGGT GGTCCAAGTA CTTTGCTTCC ATCGATACCA TGAAGGAGCA ACTTCGGCAG
    CAAGAGGTCT CAGGCACTGA CTTGGAGGAG AAAGAGGAGG TGGACAACAC CGAGGGCCTG
    AAGGGACCGA TGAAGGTCAA GGAGAAGGCA AAGGCAGCAA AGGAAGAGAA GAAAAAGAGA
    ACCCAGGGCC CCGGCGCCGG CCAGGGGTCT GAAGTCCCTG AGAAGAAGAA ACCCAAGATT
    GATGAGCTGA AGGTGTACTC CAAGGAGCTG GAGTCCGAGT TCGACAACTT TGAGGACTGG
    CTGCACACTT TCAACCTGCT GCGGGGCAAG ACGGGAGATG ATGAGGACGG CTCCACTGAG
    GAGGAGCGCA TCGTGGGCAG GTTCAAGGGC TCCCTCTGCG TGTACAAAGT GCCACTCCCA
    GAGGATGTGT CCCGGGAAGC CGGCTACGAC CCCACCTATG GCATGTTCCA GGGCATTCCC
    AGTAATGACC CCATCAATGT GCTGGTCCGA GTCTATGTGG TCAGGGCCAC GGACCTGCAC
    CCCGCAGACA TCAACGGCAA AGCAGACCCC TACATCGCCA TCCGGCTGGG CAAGACAGAC
    ATCCGGGACA AGGAGAACTA CATCTCTAAG CAGCTCAACC CTGTCTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAATCC ATGCTGACGG TGGCCGTGTA CGACTGGGAC
    CTGGTCGGCA CCGACGACCT CATTGGGGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCACC GCGCCACCTG CGGCATCGCC CAGACCTACT CCATACATGG CTACAATATC
    TGGCGGGACC CCATGAAGCC CAGCCAGATT CTGACCCGTC TCTGCAAGGA GGGCAAAGTG
    GATGGCCCCC ACTTTGGGCC CCCCGGGAGA GTGAAAGTGT CCAACCGCGT CTTCACTGGG
    CCCTCCGAGA TCGAGGACGA GAATGGTCAG AGGAAGCCCA CAGAAGAGCA CGTGGCACTG
    TCTGCCCTAA GGCACTGGGA GGACATCCCC CGTGTGGGCT GCCGCCTCGT GCCTGAACAT
    GTGGAGACTC GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TCGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ATATGTTCCC CATGGACATG CCAGCACCCG GCACACCACT GGACATCTCC
    CCTCGGAAGC CCAAGAAGTA CGAGCTCCGG GTCATCGTGT GGAACACGGA CGAGGTGGTC
    CTGGAGGACG ATGACTTCTT TACTGGGGAG AAGTCCAGCG ACATTTTTGT GCGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAGCAGGAC ACGGATGTCC ACTATCACTC CCTCACCGGC
    GAGGGCAACT TCAACTGGCG CTACCTGTTC CCCTTCGACT ATCTGGCCGC CGAGGAAAAG
    ATTGTCATCT CCAAGAAGGA GTCCATGTTC TCCTGGGACG AGACGGAGTA CAAGATCCCC
    GCGCGGCTCA CCTTGCAGAT CTGGGACGCC GACCACTTCT CCGCGGACGA TTTCCTGGGG
    GCCATCGAGC TGGACCTGAA CCGGTTCCCC CGGGGCGCGA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACGGGGGA GGTGGACGTG CCCCTGGTTT CCATCTTCAA GCAGAAGCGC
    GTCAAAGGCT GGTGGCCGCT CCTGGCCCGC GATGAGAATG ATGAGTTCGA GCTCACGGGC
    AAGGTGGAGG CAGAGTTGCA TCTCCTGACA GCCGAGGAGG CAGAGAAGAA CCCCGTGGGC
    CTGGCCCGCA ATGAACCTGA CCCCCTAGAG AAACCCAAGC GGCCAGACAC GAGCTTCATC
    TGGTTCTTGA ACCCTCTCAA GTCTGCCCGC TACTTCTTGT GGCACACATA CCGCTGGCTG
    CTCCTCAAAC TGCTGCTGCT CATGCTGCTG CTCCTCCTCC TCGCCCTCTT CCTCTACTCT
    GTGCCCGGCT ACCTGGTCAA GAAGATCCTG GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.