LOC491954 cDNA ORF clone, Canis lupus familiaris(dog)

The following LOC491954 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC491954 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe285310 XM_022415945.1
Latest version!
Canis lupus familiaris NHS like 2 (LOC491954), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe285311 XM_022415946.1
Latest version!
Canis lupus familiaris NHS like 2 (LOC491954), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe285309 XM_022415943.1
Latest version!
Canis lupus familiaris NHS like 2 (LOC491954), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe45522 XM_014111505.1
Latest version!
Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45523 XM_014111506.1
Latest version!
Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe45524 XM_014111507.1
Latest version!
Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe45525 XM_014111508.1
Latest version!
Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe45526 XM_014111509.1
Latest version!
Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe32539 XM_549074.5
Latest version!
Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe32540 XM_005641480.3
Latest version!
Canis lupus familiaris NHS like 2 (LOC491954), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe32541 XM_005641481.2
Latest version!
Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe32541 XM_005641482.2
Latest version!
Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe32541 XM_022415944.1
Latest version!
Canis lupus familiaris NHS like 2 (LOC491954), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe32542 XM_005641483.2
Latest version!
Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe32540 XM_005641480.2 Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe285310
    Clone ID Related Accession (Same CDS sequence) XM_022415945.1
    Accession Version XM_022415945.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NHS-like protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAGAG CCGAGTTTGT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGAGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGG TCATAGCAAC AGGCCTGCAG GCAGTGTGGC CCACTCTGCC
    ACCTCAGACA TCAAGTCCAG TCATTCCATC CCAGAAGGGG TTCATGGAAG AATTGCCATT
    GGTCAGGAAG CTCAGTTCCC AAATCTCACC TCACCAGTAC TGAGGAGTCC TTCTAGTGAT
    TCAGAAGAAC CTCTCCAGGC ATGTGGTGGC ACAAACCCTC CTGGTATGGA GAGCATAGGA
    ATGGTGTATA GCATCCCCAG TTCTTGCAAT GGACCAACGG AATCAACGTT CTCTGCTTCC
    TGGAAGGGAG ATACTTTTAC CTACATGACT CCAAGTGTCA CCAGTCAGAA CAATCAAATC
    AGTCAAAATG GAAGAAATCC TTCCTCTGGG AATGCTTGGG TCTCTCTGCA CACACTGCCA
    CCTCTAGTCC CCAAGGAGGC CACTACCTTC TTTGTTACCC GAGAGAACCC AACAGGATGC
    AGTGGGGCAA CTGGCAACTC TGAGCATCCT ACTCAACGAA GGCAAGTATC AGAGCGACCC
    TCCAAGATGG GCCTTCTCAC CAGTGGTACC TCAAGGCTGG AGACAGGCCG GGGGGGTGCC
    AGCAGGTTCC GGGAGCGGTC ACTGTCTGTG CCCATGGACT CTGGCACCAC GGATGTGGAC
    TATGATGAGG AACAGAAGGC CAGTGAAGCC TGTGCCCTGC CTTATGCCAG TACAAGCTCT
    GAGGGCAGTA ACAGTACTGA CAACATTGCC TCCCTTAGCA CCCAACAAGA GGCCCAGCCC
    AGAAGGCAGA GATCCAAGAG TATCTCACTC CGGAAGGCCA AGAAGAAGCC TTCCCCACCG
    ATGCGCAGTG TCTCACTGCT CAAAGATGGG CCAGTCCTCT TGCCAGAACA TGGGTCAGCG
    CTGCCCAAGG ACCAGAGGCC CAGGAGTCTC TGCCTCTCCT TGGAACACCA AGGGCGTCAG
    TCATCCCACC CAGATGCTCA GGGTCACCCA GCTGTGCCAA CCGTCAAAGA TCCAGAAGGT
    GCACAATTCT CCCACCACTG GTATCCTACT AACTGGAAGT CTGGCGACAC CTACCAATCC
    TTGTCCAGTT CCAGCACGGC CACTGGCACA ACAGTCATCG AGTGCACTCA AGTTCAGGGA
    AGCTCAGAGT CTCTTGCCTC CCCTTCCACA TCCAGAGCCA CGACACCTTC CCAACTCTCT
    ATCGAGGTGG AGGCCAGGGA AGTGTCCTCC CCAGGAAGGC CCACTGGGCT GATGTCACCT
    TCCAGTGGCT ACTCCAGCCA ATCAGAGACA CCAACACCCA CTGTCTCCAT GTCCTTGACC
    CTGGGCCACC TGCCCCCTCC AAGTGGCAGT GTCCGGGTGC GGCCAGTGGT ACCCGAGAGG
    AAGTCATCAC TCCCCCCGAC ATCGCCTATG GAGAAAATCT CCAAGTCCCG GTTGTCCTTT
    GACCTGCCAC TGACCTCCTC AACCAACCTG GATCTGTCTG GGATGAGCAT CTCCATCCGA
    AGCAAAACCA AGGTGAGCCG GCATCATTCA GATACAAATT TTGGGGCTAA GCTGGCCCTG
    AAAAAGAGCC CCAACCAGCC ATTTATGCCC ATGGTCACTC AGTCTGACCT ACGTTCCATT
    CACCTGAGGT CTGTCAGCAA GTCTGAGCCG GAAGATGACA TCGAGAGCCC TGACTATGCT
    GAGGACGCAG GAGCAGAGGT CTTCGTATTG CTGGAGAGAA AGATGAAACC TCCCATAGCC
    GAGAAGCCCC TTGTGGCCCG AAGTCCTCCC AGCTTGGTCC ACAAGCCACC ATCTGCCCCT
    GAGGAGTACC CACTCACTTC ACCGACCTTG GCTGTGACCC CCAAGATCTC TGTTCAACAC
    ATGCGGCCAC TCCCCAAAGA CATCTACACG GTGATGCGGA AACCAAAGTC CCCCGGCTTC
    CCCGAGGGCA GAGGCTCCAG GGAGCCACCA GCGCCCTCTT CTCTTGCTTT CACACCTTTT
    GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA ACACAGCAAC CTCCCCAGGG CACACTGGAG
    GATGGGGGCC CCAAGGTGAG AGCCCTGCCT GAAAGAATCA GCCTCCAGAG CCAGGAAGAA
    GCAGAGAAAA AGAAAGGCAA GATTCCACCT CCTGTCCCCA AAAAACCCAG CGTGCTGTAC
    CTGCCTCTCA CATCACCCAC AGCACACATG GAGGTGTTCA GGGCCGAACT GAGGCTGCCC
    CACAGCCCCA TCATCACCCT GCAGGAAGAC ACCAAGTGTC CGCCCACCAG TGACCCCCTG
    AAGGCACCTG GTGTGAGGCC AACTTCACCG CTGCAGGCTG AAGGTGAAAG GGAGGCAAGT
    CCTCTAGGGA GTTCTGTGGA ACCGAGCACT GCAGAAAAAA GCATAATCAG TGACAAAACA
    GCTGAATGGA TCGCCGAAGA TGATGATGAC GTGTTTGTGG CTTCACGCAC AACCGAAGAT
    TTATTTACTG TGATACACAG GTCCAAAAGA AAGCTGCTTG GCTGGAAAGA GCCTGGGGAG
    GCCTTTGCTG GTGGCAGCAG ATCAAGCTCC CATTCACCAA TAAAGAACAC AGCTGATTTT
    TCCATCAGTG AGTCAGCTGT CTCTGCGGGG TCGAGTGGCA GCGCCAACCT GGATGCTGGC
    AGAAACAATG ATTTCAAGGC CTTGCTGCAA AAGAAGGGAA GCAAGGCAAC CCCCAAGTCC
    CGCCCCTCAG CTGCTGAACT GCTCAAGACC ACTAATCCGC TGGCTCGGAG AATTATTGCA
    CAATTTTCCA AAGAGTATGA GACCACCAAC AACCCTGGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271653.1
    CDS496..3480
    Translation

    Target ORF information:

    RefSeq Version XM_022415945.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NHS like 2 (LOC491954), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415945.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAGAG CCGAGTTTGT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGAGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGG TCATAGCAAC AGGCCTGCAG GCAGTGTGGC CCACTCTGCC
    ACCTCAGACA TCAAGTCCAG TCATTCCATC CCAGAAGGGG TTCATGGAAG AATTGCCATT
    GGTCAGGAAG CTCAGTTCCC AAATCTCACC TCACCAGTAC TGAGGAGTCC TTCTAGTGAT
    TCAGAAGAAC CTCTCCAGGC ATGTGGTGGC ACAAACCCTC CTGGTATGGA GAGCATAGGA
    ATGGTGTATA GCATCCCCAG TTCTTGCAAT GGACCAACGG AATCAACGTT CTCTGCTTCC
    TGGAAGGGAG ATACTTTTAC CTACATGACT CCAAGTGTCA CCAGTCAGAA CAATCAAATC
    AGTCAAAATG GAAGAAATCC TTCCTCTGGG AATGCTTGGG TCTCTCTGCA CACACTGCCA
    CCTCTAGTCC CCAAGGAGGC CACTACCTTC TTTGTTACCC GAGAGAACCC AACAGGATGC
    AGTGGGGCAA CTGGCAACTC TGAGCATCCT ACTCAACGAA GGCAAGTATC AGAGCGACCC
    TCCAAGATGG GCCTTCTCAC CAGTGGTACC TCAAGGCTGG AGACAGGCCG GGGGGGTGCC
    AGCAGGTTCC GGGAGCGGTC ACTGTCTGTG CCCATGGACT CTGGCACCAC GGATGTGGAC
    TATGATGAGG AACAGAAGGC CAGTGAAGCC TGTGCCCTGC CTTATGCCAG TACAAGCTCT
    GAGGGCAGTA ACAGTACTGA CAACATTGCC TCCCTTAGCA CCCAACAAGA GGCCCAGCCC
    AGAAGGCAGA GATCCAAGAG TATCTCACTC CGGAAGGCCA AGAAGAAGCC TTCCCCACCG
    ATGCGCAGTG TCTCACTGCT CAAAGATGGG CCAGTCCTCT TGCCAGAACA TGGGTCAGCG
    CTGCCCAAGG ACCAGAGGCC CAGGAGTCTC TGCCTCTCCT TGGAACACCA AGGGCGTCAG
    TCATCCCACC CAGATGCTCA GGGTCACCCA GCTGTGCCAA CCGTCAAAGA TCCAGAAGGT
    GCACAATTCT CCCACCACTG GTATCCTACT AACTGGAAGT CTGGCGACAC CTACCAATCC
    TTGTCCAGTT CCAGCACGGC CACTGGCACA ACAGTCATCG AGTGCACTCA AGTTCAGGGA
    AGCTCAGAGT CTCTTGCCTC CCCTTCCACA TCCAGAGCCA CGACACCTTC CCAACTCTCT
    ATCGAGGTGG AGGCCAGGGA AGTGTCCTCC CCAGGAAGGC CCACTGGGCT GATGTCACCT
    TCCAGTGGCT ACTCCAGCCA ATCAGAGACA CCAACACCCA CTGTCTCCAT GTCCTTGACC
    CTGGGCCACC TGCCCCCTCC AAGTGGCAGT GTCCGGGTGC GGCCAGTGGT ACCCGAGAGG
    AAGTCATCAC TCCCCCCGAC ATCGCCTATG GAGAAAATCT CCAAGTCCCG GTTGTCCTTT
    GACCTGCCAC TGACCTCCTC AACCAACCTG GATCTGTCTG GGATGAGCAT CTCCATCCGA
    AGCAAAACCA AGGTGAGCCG GCATCATTCA GATACAAATT TTGGGGCTAA GCTGGCCCTG
    AAAAAGAGCC CCAACCAGCC ATTTATGCCC ATGGTCACTC AGTCTGACCT ACGTTCCATT
    CACCTGAGGT CTGTCAGCAA GTCTGAGCCG GAAGATGACA TCGAGAGCCC TGACTATGCT
    GAGGACGCAG GAGCAGAGGT CTTCGTATTG CTGGAGAGAA AGATGAAACC TCCCATAGCC
    GAGAAGCCCC TTGTGGCCCG AAGTCCTCCC AGCTTGGTCC ACAAGCCACC ATCTGCCCCT
    GAGGAGTACC CACTCACTTC ACCGACCTTG GCTGTGACCC CCAAGATCTC TGTTCAACAC
    ATGCGGCCAC TCCCCAAAGA CATCTACACG GTGATGCGGA AACCAAAGTC CCCCGGCTTC
    CCCGAGGGCA GAGGCTCCAG GGAGCCACCA GCGCCCTCTT CTCTTGCTTT CACACCTTTT
    GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA ACACAGCAAC CTCCCCAGGG CACACTGGAG
    GATGGGGGCC CCAAGGTGAG AGCCCTGCCT GAAAGAATCA GCCTCCAGAG CCAGGAAGAA
    GCAGAGAAAA AGAAAGGCAA GATTCCACCT CCTGTCCCCA AAAAACCCAG CGTGCTGTAC
    CTGCCTCTCA CATCACCCAC AGCACACATG GAGGTGTTCA GGGCCGAACT GAGGCTGCCC
    CACAGCCCCA TCATCACCCT GCAGGAAGAC ACCAAGTGTC CGCCCACCAG TGACCCCCTG
    AAGGCACCTG GTGTGAGGCC AACTTCACCG CTGCAGGCTG AAGGTGAAAG GGAGGCAAGT
    CCTCTAGGGA GTTCTGTGGA ACCGAGCACT GCAGAAAAAA GCATAATCAG TGACAAAACA
    GCTGAATGGA TCGCCGAAGA TGATGATGAC GTGTTTGTGG CTTCACGCAC AACCGAAGAT
    TTATTTACTG TGATACACAG GTCCAAAAGA AAGCTGCTTG GCTGGAAAGA GCCTGGGGAG
    GCCTTTGCTG GTGGCAGCAG ATCAAGCTCC CATTCACCAA TAAAGAACAC AGCTGATTTT
    TCCATCAGTG AGTCAGCTGT CTCTGCGGGG TCGAGTGGCA GCGCCAACCT GGATGCTGGC
    AGAAACAATG ATTTCAAGGC CTTGCTGCAA AAGAAGGGAA GCAAGGCAAC CCCCAAGTCC
    CGCCCCTCAG CTGCTGAACT GCTCAAGACC ACTAATCCGC TGGCTCGGAG AATTATTGCA
    CAATTTTCCA AAGAGTATGA GACCACCAAC AACCCTGGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285311
    Clone ID Related Accession (Same CDS sequence) XM_022415946.1
    Accession Version XM_022415946.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NHS-like protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGAGAG CCGAGTTTGT CACCCTCTTC TGGAGCCGGG GAGGTCATAG CAACAGGCCT 
    GCAGGCAGTG TGGCCCACTC TGCCACCTCA GACATCAAGT CCAGTCATTC CATCCCAGAA
    GGGGTTCATG GAAGAATTGC CATTGGTCAG GAAGCTCAGT TCCCAAATCT CACCTCACCA
    GTACTGAGGA GTCCTTCTAG TGATTCAGAA GAACCTCTCC AGGCATGTGG TGGCACAAAC
    CCTCCTGGTA TGGAGAGCAT AGGAATGGTG TATAGCATCC CCAGTTCTTG CAATGGACCA
    ACGGAATCAA CGTTCTCTGC TTCCTGGAAG GGAGATACTT TTACCTACAT GACTCCAAGT
    GTCACCAGTC AGAACAATCA AATCAGTCAA AATGGAAGAA ATCCTTCCTC TGGGAATGCT
    TGGGTCTCTC TGCACACACT GCCACCTCTA GTCCCCAAGG AGGCCACTAC CTTCTTTGTT
    ACCCGAGAGA ACCCAACAGG ATGCAGTGGG GCAACTGGCA ACTCTGAGCA TCCTACTCAA
    CGAAGGCAAG TATCAGAGCG ACCCTCCAAG ATGGGCCTTC TCACCAGTGG TACCTCAAGG
    CTGGAGACAG GCCGGGGGGG TGCCAGCAGG TTCCGGGAGC GGTCACTGTC TGTGCCCATG
    GACTCTGGCA CCACGGATGT GGACTATGAT GAGGAACAGA AGGCCAGTGA AGCCTGTGCC
    CTGCCTTATG CCAGTACAAG CTCTGAGGGC AGTAACAGTA CTGACAACAT TGCCTCCCTT
    AGCACCCAAC AAGAGGCCCA GCCCAGAAGG CAGAGATCCA AGAGTATCTC ACTCCGGAAG
    GCCAAGAAGA AGCCTTCCCC ACCGATGCGC AGTGTCTCAC TGCTCAAAGA TGGGCCAGTC
    CTCTTGCCAG AACATGGGTC AGCGCTGCCC AAGGACCAGA GGCCCAGGAG TCTCTGCCTC
    TCCTTGGAAC ACCAAGGGCG TCAGTCATCC CACCCAGATG CTCAGGGTCA CCCAGCTGTG
    CCAACCGTCA AAGATCCAGA AGGTGCACAA TTCTCCCACC ACTGGTATCC TACTAACTGG
    AAGTCTGGCG ACACCTACCA ATCCTTGTCC AGTTCCAGCA CGGCCACTGG CACAACAGTC
    ATCGAGTGCA CTCAAGTTCA GGGAAGCTCA GAGTCTCTTG CCTCCCCTTC CACATCCAGA
    GCCACGACAC CTTCCCAACT CTCTATCGAG GTGGAGGCCA GGGAAGTGTC CTCCCCAGGA
    AGGCCCACTG GGCTGATGTC ACCTTCCAGT GGCTACTCCA GCCAATCAGA GACACCAACA
    CCCACTGTCT CCATGTCCTT GACCCTGGGC CACCTGCCCC CTCCAAGTGG CAGTGTCCGG
    GTGCGGCCAG TGGTACCCGA GAGGAAGTCA TCACTCCCCC CGACATCGCC TATGGAGAAA
    ATCTCCAAGT CCCGGTTGTC CTTTGACCTG CCACTGACCT CCTCAACCAA CCTGGATCTG
    TCTGGGATGA GCATCTCCAT CCGAAGCAAA ACCAAGGTGA GCCGGCATCA TTCAGATACA
    AATTTTGGGG CTAAGCTGGC CCTGAAAAAG AGCCCCAACC AGCCATTTAT GCCCATGGTC
    ACTCAGTCTG ACCTACGTTC CATTCACCTG AGGTCTGTCA GCAAGTCTGA GCCGGAAGAT
    GACATCGAGA GCCCTGACTA TGCTGAGGAC GCAGGAGCAG AGGTCTTCGT ATTGCTGGAG
    AGAAAGATGA AACCTCCCAT AGCCGAGAAG CCCCTTGTGG CCCGAAGTCC TCCCAGCTTG
    GTCCACAAGC CACCATCTGC CCCTGAGGAG TACCCACTCA CTTCACCGAC CTTGGCTGTG
    ACCCCCAAGA TCTCTGTTCA ACACATGCGG CCACTCCCCA AAGACATCTA CACGGTGATG
    CGGAAACCAA AGTCCCCCGG CTTCCCCGAG GGCAGAGGCT CCAGGGAGCC ACCAGCGCCC
    TCTTCTCTTG CTTTCACACC TTTTGCCAGT TCCTCTGGTG CTTTCTTCTC AGGAACACAG
    CAACCTCCCC AGGGCACACT GGAGGATGGG GGCCCCAAGG TGAGAGCCCT GCCTGAAAGA
    ATCAGCCTCC AGAGCCAGGA AGAAGCAGAG AAAAAGAAAG GCAAGATTCC ACCTCCTGTC
    CCCAAAAAAC CCAGCGTGCT GTACCTGCCT CTCACATCAC CCACAGCACA CATGGAGGTG
    TTCAGGGCCG AACTGAGGCT GCCCCACAGC CCCATCATCA CCCTGCAGGA AGACACCAAG
    TGTCCGCCCA CCAGTGACCC CCTGAAGGCA CCTGGTGTGA GGCCAACTTC ACCGCTGCAG
    GCTGAAGGTG AAAGGGAGGC AAGTCCTCTA GGGAGTTCTG TGGAACCGAG CACTGCAGAA
    AAAAGCATAA TCAGTGACAA AACAGCTGAA TGGATCGCCG AAGATGATGA TGACGTGTTT
    GTGGCTTCAC GCACAACCGA AGATTTATTT ACTGTGATAC ACAGGTCCAA AAGAAAGCTG
    CTTGGCTGGA AAGAGCCTGG GGAGGCCTTT GCTGGTGGCA GCAGATCAAG CTCCCATTCA
    CCAATAAAGA ACACAGCTGA TTTTTCCATC AGTGAGTCAG CTGTCTCTGC GGGGTCGAGT
    GGCAGCGCCA ACCTGGATGC TGGCAGAAAC AATGATTTCA AGGCCTTGCT GCAAAAGAAG
    GGAAGCAAGG CAACCCCCAA GTCCCGCCCC TCAGCTGCTG AACTGCTCAA GACCACTAAT
    CCGCTGGCTC GGAGAATTAT TGCACAATTT TCCAAAGAGT ATGAGACCAC CAACAACCCT
    GGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271654.1
    CDS494..3322
    Translation

    Target ORF information:

    RefSeq Version XM_022415946.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NHS like 2 (LOC491954), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415946.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGAGAG CCGAGTTTGT CACCCTCTTC TGGAGCCGGG GAGGTCATAG CAACAGGCCT 
    GCAGGCAGTG TGGCCCACTC TGCCACCTCA GACATCAAGT CCAGTCATTC CATCCCAGAA
    GGGGTTCATG GAAGAATTGC CATTGGTCAG GAAGCTCAGT TCCCAAATCT CACCTCACCA
    GTACTGAGGA GTCCTTCTAG TGATTCAGAA GAACCTCTCC AGGCATGTGG TGGCACAAAC
    CCTCCTGGTA TGGAGAGCAT AGGAATGGTG TATAGCATCC CCAGTTCTTG CAATGGACCA
    ACGGAATCAA CGTTCTCTGC TTCCTGGAAG GGAGATACTT TTACCTACAT GACTCCAAGT
    GTCACCAGTC AGAACAATCA AATCAGTCAA AATGGAAGAA ATCCTTCCTC TGGGAATGCT
    TGGGTCTCTC TGCACACACT GCCACCTCTA GTCCCCAAGG AGGCCACTAC CTTCTTTGTT
    ACCCGAGAGA ACCCAACAGG ATGCAGTGGG GCAACTGGCA ACTCTGAGCA TCCTACTCAA
    CGAAGGCAAG TATCAGAGCG ACCCTCCAAG ATGGGCCTTC TCACCAGTGG TACCTCAAGG
    CTGGAGACAG GCCGGGGGGG TGCCAGCAGG TTCCGGGAGC GGTCACTGTC TGTGCCCATG
    GACTCTGGCA CCACGGATGT GGACTATGAT GAGGAACAGA AGGCCAGTGA AGCCTGTGCC
    CTGCCTTATG CCAGTACAAG CTCTGAGGGC AGTAACAGTA CTGACAACAT TGCCTCCCTT
    AGCACCCAAC AAGAGGCCCA GCCCAGAAGG CAGAGATCCA AGAGTATCTC ACTCCGGAAG
    GCCAAGAAGA AGCCTTCCCC ACCGATGCGC AGTGTCTCAC TGCTCAAAGA TGGGCCAGTC
    CTCTTGCCAG AACATGGGTC AGCGCTGCCC AAGGACCAGA GGCCCAGGAG TCTCTGCCTC
    TCCTTGGAAC ACCAAGGGCG TCAGTCATCC CACCCAGATG CTCAGGGTCA CCCAGCTGTG
    CCAACCGTCA AAGATCCAGA AGGTGCACAA TTCTCCCACC ACTGGTATCC TACTAACTGG
    AAGTCTGGCG ACACCTACCA ATCCTTGTCC AGTTCCAGCA CGGCCACTGG CACAACAGTC
    ATCGAGTGCA CTCAAGTTCA GGGAAGCTCA GAGTCTCTTG CCTCCCCTTC CACATCCAGA
    GCCACGACAC CTTCCCAACT CTCTATCGAG GTGGAGGCCA GGGAAGTGTC CTCCCCAGGA
    AGGCCCACTG GGCTGATGTC ACCTTCCAGT GGCTACTCCA GCCAATCAGA GACACCAACA
    CCCACTGTCT CCATGTCCTT GACCCTGGGC CACCTGCCCC CTCCAAGTGG CAGTGTCCGG
    GTGCGGCCAG TGGTACCCGA GAGGAAGTCA TCACTCCCCC CGACATCGCC TATGGAGAAA
    ATCTCCAAGT CCCGGTTGTC CTTTGACCTG CCACTGACCT CCTCAACCAA CCTGGATCTG
    TCTGGGATGA GCATCTCCAT CCGAAGCAAA ACCAAGGTGA GCCGGCATCA TTCAGATACA
    AATTTTGGGG CTAAGCTGGC CCTGAAAAAG AGCCCCAACC AGCCATTTAT GCCCATGGTC
    ACTCAGTCTG ACCTACGTTC CATTCACCTG AGGTCTGTCA GCAAGTCTGA GCCGGAAGAT
    GACATCGAGA GCCCTGACTA TGCTGAGGAC GCAGGAGCAG AGGTCTTCGT ATTGCTGGAG
    AGAAAGATGA AACCTCCCAT AGCCGAGAAG CCCCTTGTGG CCCGAAGTCC TCCCAGCTTG
    GTCCACAAGC CACCATCTGC CCCTGAGGAG TACCCACTCA CTTCACCGAC CTTGGCTGTG
    ACCCCCAAGA TCTCTGTTCA ACACATGCGG CCACTCCCCA AAGACATCTA CACGGTGATG
    CGGAAACCAA AGTCCCCCGG CTTCCCCGAG GGCAGAGGCT CCAGGGAGCC ACCAGCGCCC
    TCTTCTCTTG CTTTCACACC TTTTGCCAGT TCCTCTGGTG CTTTCTTCTC AGGAACACAG
    CAACCTCCCC AGGGCACACT GGAGGATGGG GGCCCCAAGG TGAGAGCCCT GCCTGAAAGA
    ATCAGCCTCC AGAGCCAGGA AGAAGCAGAG AAAAAGAAAG GCAAGATTCC ACCTCCTGTC
    CCCAAAAAAC CCAGCGTGCT GTACCTGCCT CTCACATCAC CCACAGCACA CATGGAGGTG
    TTCAGGGCCG AACTGAGGCT GCCCCACAGC CCCATCATCA CCCTGCAGGA AGACACCAAG
    TGTCCGCCCA CCAGTGACCC CCTGAAGGCA CCTGGTGTGA GGCCAACTTC ACCGCTGCAG
    GCTGAAGGTG AAAGGGAGGC AAGTCCTCTA GGGAGTTCTG TGGAACCGAG CACTGCAGAA
    AAAAGCATAA TCAGTGACAA AACAGCTGAA TGGATCGCCG AAGATGATGA TGACGTGTTT
    GTGGCTTCAC GCACAACCGA AGATTTATTT ACTGTGATAC ACAGGTCCAA AAGAAAGCTG
    CTTGGCTGGA AAGAGCCTGG GGAGGCCTTT GCTGGTGGCA GCAGATCAAG CTCCCATTCA
    CCAATAAAGA ACACAGCTGA TTTTTCCATC AGTGAGTCAG CTGTCTCTGC GGGGTCGAGT
    GGCAGCGCCA ACCTGGATGC TGGCAGAAAC AATGATTTCA AGGCCTTGCT GCAAAAGAAG
    GGAAGCAAGG CAACCCCCAA GTCCCGCCCC TCAGCTGCTG AACTGCTCAA GACCACTAAT
    CCGCTGGCTC GGAGAATTAT TGCACAATTT TCCAAAGAGT ATGAGACCAC CAACAACCCT
    GGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285309
    Clone ID Related Accession (Same CDS sequence) XM_022415943.1
    Accession Version XM_022415943.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3309bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NHS-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGAGAG CCGAGTTTGT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGAGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTACT CAGAGGCCAG ACTTCTGGGG
    CAGACCTTCC GCTCTGCTGA TGAGGCCCCC GAGGCCACTC CTAGCCCAAG GCCCCAGGCT
    GGCAGGCGTC TGGAGCTTGT ATTGATGCCT ACAAAACGGC AGCTGAGCGA GGATGAGACT
    ACCACCCAGG GTGTGAGGGC CCCTGAGGCC TCCTCGAGCC TGTCTACCAC AGCCGACAAG
    CAAGCTGCCT GGAATAGCCC CTTCCCTCTG CCCATCCTAG AGGAGAAGCG GTGGCTTCAG
    CCTTGCTCCA CGCAATCTGA CATTGTGCCC ATCAACATCT CTGGTCATAG CAACAGGCCT
    GCAGGCAGTG TGGCCCACTC TGCCACCTCA GACATCAAGT CCAGTCATTC CATCCCAGAA
    GGGGTTCATG GAAGAATTGC CATTGGTCAG GAAGCTCAGT TCCCAAATCT CACCTCACCA
    GTACTGAGGA GTCCTTCTAG TGATTCAGAA GAACCTCTCC AGGCATGTGG TGGCACAAAC
    CCTCCTGGTA TGGAGAGCAT AGGAATGGTG TATAGCATCC CCAGTTCTTG CAATGGACCA
    ACGGAATCAA CGTTCTCTGC TTCCTGGAAG GGAGATACTT TTACCTACAT GACTCCAAGT
    GTCACCAGTC AGAACAATCA AATCAGTCAA AATGGAAGAA ATCCTTCCTC TGGGAATGCT
    TGGGTCTCTC TGCACACACT GCCACCTCTA GTCCCCAAGG AGGCCACTAC CTTCTTTGTT
    ACCCGAGAGA ACCCAACAGG ATGCAGTGGG GCAACTGGCA ACTCTGAGCA TCCTACTCAA
    CGAAGGCAAG TATCAGAGCG ACCCTCCAAG ATGGGCCTTC TCACCAGTGG TACCTCAAGG
    CTGGAGACAG GCCGGGGGGG TGCCAGCAGG TTCCGGGAGC GGTCACTGTC TGTGCCCATG
    GACTCTGGCA CCACGGATGT GGACTATGAT GAGGAACAGA AGGCCAGTGA AGCCTGTGCC
    CTGCCTTATG CCAGTACAAG CTCTGAGGGC AGTAACAGTA CTGACAACAT TGCCTCCCTT
    AGCACCCAAC AAGAGGCCCA GCCCAGAAGG CAGAGATCCA AGAGTATCTC ACTCCGGAAG
    GCCAAGAAGA AGCCTTCCCC ACCGATGCGC AGTGTCTCAC TGCTCAAAGA TGGGCCAGTC
    CTCTTGCCAG AACATGGGTC AGCGCTGCCC AAGGACCAGA GGCCCAGGAG TCTCTGCCTC
    TCCTTGGAAC ACCAAGGGCG TCAGTCATCC CACCCAGATG CTCAGGGTCA CCCAGCTGTG
    CCAACCGTCA AAGATCCAGA AGGTGCACAA TTCTCCCACC ACTGGTATCC TACTAACTGG
    AAGTCTGGCG ACACCTACCA ATCCTTGTCC AGTTCCAGCA CGGCCACTGG CACAACAGTC
    ATCGAGTGCA CTCAAGTTCA GGGAAGCTCA GAGTCTCTTG CCTCCCCTTC CACATCCAGA
    GCCACGACAC CTTCCCAACT CTCTATCGAG GTGGAGGCCA GGGAAGTGTC CTCCCCAGGA
    AGGCCCACTG GGCTGATGTC ACCTTCCAGT GGCTACTCCA GCCAATCAGA GACACCAACA
    CCCACTGTCT CCATGTCCTT GACCCTGGGC CACCTGCCCC CTCCAAGTGG CAGTGTCCGG
    GTGCGGCCAG TGGTACCCGA GAGGAAGTCA TCACTCCCCC CGACATCGCC TATGGAGAAA
    ATCTCCAAGT CCCGGTTGTC CTTTGACCTG CCACTGACCT CCTCAACCAA CCTGGATCTG
    TCTGGGATGA GCATCTCCAT CCGAAGCAAA ACCAAGGTGA GCCGGCATCA TTCAGATACA
    AATTTTGGGG CTAAGCTGGC CCTGAAAAAG AGCCCCAACC AGCCATTTAT GCCCATGGTC
    ACTCAGTCTG ACCTACGTTC CATTCACCTG AGGTCTGTCA GCAAGTCTGA GCCGGAAGAT
    GACATCGAGA GCCCTGACTA TGCTGAGGAC GCAGGAGCAG AGGTCTTCGT ATTGCTGGAG
    AGAAAGATGA AACCTCCCAT AGCCGAGAAG CCCCTTGTGG CCCGAAGTCC TCCCAGCTTG
    GTCCACAAGC CACCATCTGC CCCTGAGGAG TACCCACTCA CTTCACCGAC CTTGGCTGTG
    ACCCCCAAGA TCTCTGTTCA ACACATGCGG CCACTCCCCA AAGACATCTA CACGGTGATG
    CGGAAACCAA AGTCCCCCGG CTTCCCCGAG GGCAGAGGCT CCAGGGAGCC ACCAGCGCCC
    TCTTCTCTTG CTTTCACACC TTTTGCCAGT TCCTCTGGTG CTTTCTTCTC AGGAACACAG
    CAACCTCCCC AGGGCACACT GGAGGATGGG GGCCCCAAGG TGAGAGCCCT GCCTGAAAGA
    ATCAGCCTCC AGAGCCAGGA AGAAGCAGAG AAAAAGAAAG GCAAGATTCC ACCTCCTGTC
    CCCAAAAAAC CCAGCGTGCT GTACCTGCCT CTCACATCAC CCACAGCACA CATGGAGGTG
    TTCAGGGCCG AACTGAGGCT GCCCCACAGC CCCATCATCA CCCTGCAGGA AGACACCAAG
    TGTCCGCCCA CCAGTGACCC CCTGAAGGCA CCTGGTGTGA GGCCAACTTC ACCGCTGCAG
    GCTGAAGGTG AAAGGGAGGC AAGTCCTCTA GGGAGTTCTG TGGAACCGAG CACTGCAGAA
    AAAAGCATAA TCAGTGACAA AACAGCTGAA TGGATCGCCG AAGATGATGA TGACGTGTTT
    GTGGCTTCAC GCACAACCGA AGATTTATTT ACTGTGATAC ACAGGTCCAA AAGAAAGCTG
    CTTGGCTGGA AAGAGCCTGG GGAGGCCTTT GCTGGTGGCA GCAGATCAAG CTCCCATTCA
    CCAATAAAGA ACACAGCTGA TTTTTCCATC AGTGAGTCAG CTGTCTCTGC GGGGTCGAGT
    GGCAGCGCCA ACCTGGATGC TGGCAGAAAC AATGATTTCA AGGCCTTGCT GCAAAAGAAG
    GGAAGCAAGG CAACCCCCAA GTCCCGCCCC TCAGCTGCTG AACTGCTCAA GACCACTAAT
    CCGCTGGCTC GGAGAATTAT TGCACAATTT TCCAAAGAGT ATGAGACCAC CAACAACCCT
    GGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271651.1
    CDS499..3807
    Translation

    Target ORF information:

    RefSeq Version XM_022415943.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NHS like 2 (LOC491954), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415943.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGAGAG CCGAGTTTGT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGAGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTACT CAGAGGCCAG ACTTCTGGGG
    CAGACCTTCC GCTCTGCTGA TGAGGCCCCC GAGGCCACTC CTAGCCCAAG GCCCCAGGCT
    GGCAGGCGTC TGGAGCTTGT ATTGATGCCT ACAAAACGGC AGCTGAGCGA GGATGAGACT
    ACCACCCAGG GTGTGAGGGC CCCTGAGGCC TCCTCGAGCC TGTCTACCAC AGCCGACAAG
    CAAGCTGCCT GGAATAGCCC CTTCCCTCTG CCCATCCTAG AGGAGAAGCG GTGGCTTCAG
    CCTTGCTCCA CGCAATCTGA CATTGTGCCC ATCAACATCT CTGGTCATAG CAACAGGCCT
    GCAGGCAGTG TGGCCCACTC TGCCACCTCA GACATCAAGT CCAGTCATTC CATCCCAGAA
    GGGGTTCATG GAAGAATTGC CATTGGTCAG GAAGCTCAGT TCCCAAATCT CACCTCACCA
    GTACTGAGGA GTCCTTCTAG TGATTCAGAA GAACCTCTCC AGGCATGTGG TGGCACAAAC
    CCTCCTGGTA TGGAGAGCAT AGGAATGGTG TATAGCATCC CCAGTTCTTG CAATGGACCA
    ACGGAATCAA CGTTCTCTGC TTCCTGGAAG GGAGATACTT TTACCTACAT GACTCCAAGT
    GTCACCAGTC AGAACAATCA AATCAGTCAA AATGGAAGAA ATCCTTCCTC TGGGAATGCT
    TGGGTCTCTC TGCACACACT GCCACCTCTA GTCCCCAAGG AGGCCACTAC CTTCTTTGTT
    ACCCGAGAGA ACCCAACAGG ATGCAGTGGG GCAACTGGCA ACTCTGAGCA TCCTACTCAA
    CGAAGGCAAG TATCAGAGCG ACCCTCCAAG ATGGGCCTTC TCACCAGTGG TACCTCAAGG
    CTGGAGACAG GCCGGGGGGG TGCCAGCAGG TTCCGGGAGC GGTCACTGTC TGTGCCCATG
    GACTCTGGCA CCACGGATGT GGACTATGAT GAGGAACAGA AGGCCAGTGA AGCCTGTGCC
    CTGCCTTATG CCAGTACAAG CTCTGAGGGC AGTAACAGTA CTGACAACAT TGCCTCCCTT
    AGCACCCAAC AAGAGGCCCA GCCCAGAAGG CAGAGATCCA AGAGTATCTC ACTCCGGAAG
    GCCAAGAAGA AGCCTTCCCC ACCGATGCGC AGTGTCTCAC TGCTCAAAGA TGGGCCAGTC
    CTCTTGCCAG AACATGGGTC AGCGCTGCCC AAGGACCAGA GGCCCAGGAG TCTCTGCCTC
    TCCTTGGAAC ACCAAGGGCG TCAGTCATCC CACCCAGATG CTCAGGGTCA CCCAGCTGTG
    CCAACCGTCA AAGATCCAGA AGGTGCACAA TTCTCCCACC ACTGGTATCC TACTAACTGG
    AAGTCTGGCG ACACCTACCA ATCCTTGTCC AGTTCCAGCA CGGCCACTGG CACAACAGTC
    ATCGAGTGCA CTCAAGTTCA GGGAAGCTCA GAGTCTCTTG CCTCCCCTTC CACATCCAGA
    GCCACGACAC CTTCCCAACT CTCTATCGAG GTGGAGGCCA GGGAAGTGTC CTCCCCAGGA
    AGGCCCACTG GGCTGATGTC ACCTTCCAGT GGCTACTCCA GCCAATCAGA GACACCAACA
    CCCACTGTCT CCATGTCCTT GACCCTGGGC CACCTGCCCC CTCCAAGTGG CAGTGTCCGG
    GTGCGGCCAG TGGTACCCGA GAGGAAGTCA TCACTCCCCC CGACATCGCC TATGGAGAAA
    ATCTCCAAGT CCCGGTTGTC CTTTGACCTG CCACTGACCT CCTCAACCAA CCTGGATCTG
    TCTGGGATGA GCATCTCCAT CCGAAGCAAA ACCAAGGTGA GCCGGCATCA TTCAGATACA
    AATTTTGGGG CTAAGCTGGC CCTGAAAAAG AGCCCCAACC AGCCATTTAT GCCCATGGTC
    ACTCAGTCTG ACCTACGTTC CATTCACCTG AGGTCTGTCA GCAAGTCTGA GCCGGAAGAT
    GACATCGAGA GCCCTGACTA TGCTGAGGAC GCAGGAGCAG AGGTCTTCGT ATTGCTGGAG
    AGAAAGATGA AACCTCCCAT AGCCGAGAAG CCCCTTGTGG CCCGAAGTCC TCCCAGCTTG
    GTCCACAAGC CACCATCTGC CCCTGAGGAG TACCCACTCA CTTCACCGAC CTTGGCTGTG
    ACCCCCAAGA TCTCTGTTCA ACACATGCGG CCACTCCCCA AAGACATCTA CACGGTGATG
    CGGAAACCAA AGTCCCCCGG CTTCCCCGAG GGCAGAGGCT CCAGGGAGCC ACCAGCGCCC
    TCTTCTCTTG CTTTCACACC TTTTGCCAGT TCCTCTGGTG CTTTCTTCTC AGGAACACAG
    CAACCTCCCC AGGGCACACT GGAGGATGGG GGCCCCAAGG TGAGAGCCCT GCCTGAAAGA
    ATCAGCCTCC AGAGCCAGGA AGAAGCAGAG AAAAAGAAAG GCAAGATTCC ACCTCCTGTC
    CCCAAAAAAC CCAGCGTGCT GTACCTGCCT CTCACATCAC CCACAGCACA CATGGAGGTG
    TTCAGGGCCG AACTGAGGCT GCCCCACAGC CCCATCATCA CCCTGCAGGA AGACACCAAG
    TGTCCGCCCA CCAGTGACCC CCTGAAGGCA CCTGGTGTGA GGCCAACTTC ACCGCTGCAG
    GCTGAAGGTG AAAGGGAGGC AAGTCCTCTA GGGAGTTCTG TGGAACCGAG CACTGCAGAA
    AAAAGCATAA TCAGTGACAA AACAGCTGAA TGGATCGCCG AAGATGATGA TGACGTGTTT
    GTGGCTTCAC GCACAACCGA AGATTTATTT ACTGTGATAC ACAGGTCCAA AAGAAAGCTG
    CTTGGCTGGA AAGAGCCTGG GGAGGCCTTT GCTGGTGGCA GCAGATCAAG CTCCCATTCA
    CCAATAAAGA ACACAGCTGA TTTTTCCATC AGTGAGTCAG CTGTCTCTGC GGGGTCGAGT
    GGCAGCGCCA ACCTGGATGC TGGCAGAAAC AATGATTTCA AGGCCTTGCT GCAAAAGAAG
    GGAAGCAAGG CAACCCCCAA GTCCCGCCCC TCAGCTGCTG AACTGCTCAA GACCACTAAT
    CCGCTGGCTC GGAGAATTAT TGCACAATTT TCCAAAGAGT ATGAGACCAC CAACAACCCT
    GGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45522
    Clone ID Related Accession (Same CDS sequence) XM_014111505.1
    Accession Version XM_014111505.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGCAA CCCGCCGCAG 
    CCGCTCACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCCTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GCTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAA CCTGTTCCAG CGCACTGTGC GCCTCCGCCG CCGCCTTCCC
    TGCCACCTCC TCGGCCTGGA GGACGATGAG GAAGAGCTAG CTGCAGCTAA CTCGGGTCGG
    GAAAATGCGA CAGCGACTGC CCACTCGAGG TCGTCATGGC GACAGCCAGT GAACGTGTTC
    CTCTCCTCGG GCAGGCCCCC GAGTGTAGAG GAGCTGCTTC GAGAGGCGCA GCTCAATCTC
    CAGAGCCTGT TGCAAGAAGA ATATGAGGAA CAGTACTCAG AGGCCAGACT TCTGGGGCAG
    ACCTTCCGCT CTGCTGATGA GGCCCCCGAG GCCACTCCTA GCCCAAGGCC CCAGGCTGGC
    AGGCGTCTGG AGCTTGTATT GATGCCTACA AAACGGCAGC TGAGCGAGGA TGAGACTACC
    ACCCAGGGTG TGAGGGCCCC TGAGGCCTCC TCGAGCCTGT CTACCACAGC CGACAAGCAA
    GCTGCCTGGA ATAGCCCCTT CCCTCTGCCC ATCCTAGAGG AGAAGCGGTG GCTTCAGCCT
    TGCTCCACGC AATCTGACAT TGTGCCCATC AACATCTCTG GTCATAGCAA CAGGCCTGCA
    GGCAGTGTGG CCCACTCTGC CACCTCAGAC ATCAAGTCCA GTCATTCCAT CCCAGAAGGG
    GTTCATGGAA GAATTGCCAT TGGTCAGGAA GCTCAGTTCC CAAATCTCAC CTCACCAGTA
    CTGAGGAGTC CTTCTAGTGA TTCAGAAGAA CCTCTCCAGG CATGTGGTGG CACAAACCCT
    CCTGGTATGG AGAGCATAGG AATGGTGTAT AGCATCCCCA GTTCTTGCAA TGGACCAACG
    GAATCAACGT TCTCTGCTTC CTGGAAGGGA GATACTTTTA CCTACATGAC TCCAAGTGTC
    ACCAGTCAGA ACAATCAAAT CAGTCAAAAT GGAAGAAATC CTTCCTCTGG GAATGCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTAGTC CCCAAGGAGG CCACTACCTT CTTTGTTACC
    CGAGAGAACC CAACAGGATG CAGTGGGGCA ACTGGCAACT CTGAGCATCC TACTCAACGA
    AGGCAAGTAT CAGAGCGACC CTCCAAGATG GGCCTTCTCA CCAGTGGTAC CTCAAGGCTG
    GAGACAGGCC GGGGGGGTGC CAGCAGGTTC CGGGAGCGGT CACTGTCTGT GCCCATGGAC
    TCTGGCACCA CGGATGTGGA CTATGATGAG GAACAGAAGG CCAGTGAAGC CTGTGCCCTG
    CCTTATGCCA GTACAAGCTC TGAGGGCAGT AACAGTACTG ACAACATTGC CTCCCTTAGC
    ACCCAACAAG AGGCCCAGCC CAGAAGGCAG AGATCCAAGA GTATCTCACT CCGGAAGGCC
    AAGAAGAAGC CTTCCCCACC GATGCGCAGT GTCTCACTGC TCAAAGATGG GCCAGTCCTC
    TTGCCAGAAC ATGGGTCAGC GCTGCCCAAG GACCAGAGGC CCAGGAGTCT CTGCCTCTCC
    TTGGAACACC AAGGGCGTCA GTCATCCCAC CCAGATGCTC AGGGTCACCC AGCTGTGCCA
    ACCGTCAAAG ATCCAGAAGG TGCACAATTC TCCCACCACT GGTATCCTAC TAACTGGAAG
    TCTGGCGACA CCTACCAATC CTTGTCCAGT TCCAGCACGG CCACTGGCAC AACAGTCATC
    GAGTGCACTC AAGTTCAGGG AAGCTCAGAG TCTCTTGCCT CCCCTTCCAC ATCCAGAGCC
    ACGACACCTT CCCAACTCTC TATCGAGGTG GAGGCCAGGG AAGTGTCCTC CCCAGGAAGG
    CCCACTGGGC TGATGTCACC TTCCAGTGGC TACTCCAGCC AATCAGAGAC ACCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC CTGCCCCCTC CAAGTGGCAG TGTCCGGGTG
    CGGCCAGTGG TACCCGAGAG GAAGTCATCA CTCCCCCCGA CATCGCCTAT GGAGAAAATC
    TCCAAGTCCC GGTTGTCCTT TGACCTGCCA CTGACCTCCT CAACCAACCT GGATCTGTCT
    GGGATGAGCA TCTCCATCCG AAGCAAAACC AAGGTGAGCC GGCATCATTC AGATACAAAT
    TTTGGGGCTA AGCTGGCCCT GAAAAAGAGC CCCAACCAGC CATTTATGCC CATGGTCACT
    CAGTCTGACC TACGTTCCAT TCACCTGAGG TCTGTCAGCA AGTCTGAGCC GGAAGATGAC
    ATCGAGAGCC CTGACTATGC TGAGGACGCA GGAGCAGAGG TCTTCGTATT GCTGGAGAGA
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CTTGTGGCCC GAAGTCCTCC CAGCTTGGTC
    CACAAGCCAC CATCTGCCCC TGAGGAGTAC CCACTCACTT CACCGACCTT GGCTGTGACC
    CCCAAGATCT CTGTTCAACA CATGCGGCCA CTCCCCAAAG ACATCTACAC GGTGATGCGG
    AAACCAAAGT CCCCCGGCTT CCCCGAGGGC AGAGGCTCCA GGGAGCCACC AGCGCCCTCT
    TCTCTTGCTT TCACACCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GCACACTGGA GGATGGGGGC CCCAAGGTGA GAGCCCTGCC TGAAAGAATC
    AGCCTCCAGA GCCAGGAAGA AGCAGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTCCCC
    AAAAAACCCA GCGTGCTGTA CCTGCCTCTC ACATCACCCA CAGCACACAT GGAGGTGTTC
    AGGGCCGAAC TGAGGCTGCC CCACAGCCCC ATCATCACCC TGCAGGAAGA CACCAAGTGT
    CCGCCCACCA GTGACCCCCT GAAGGCACCT GGTGTGAGGC CAACTTCACC GCTGCAGGCT
    GAAGGTGAAA GGGAGGCAAG TCCTCTAGGG AGTTCTGTGG AACCGAGCAC TGCAGAAAAA
    AGCATAATCA GTGACAAAAC AGCTGAATGG ATCGCCGAAG ATGATGATGA CGTGTTTGTG
    GCTTCACGCA CAACCGAAGA TTTATTTACT GTGATACACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAAG AGCCTGGGGA GGCCTTTGCT GGTGGCAGCA GATCAAGCTC CCATTCACCA
    ATAAAGAACA CAGCTGATTT TTCCATCAGT GAGTCAGCTG TCTCTGCGGG GTCGAGTGGC
    AGCGCCAACC TGGATGCTGG CAGAAACAAT GATTTCAAGG CCTTGCTGCA AAAGAAGGGA
    AGCAAGGCAA CCCCCAAGTC CCGCCCCTCA GCTGCTGAAC TGCTCAAGAC CACTAATCCG
    CTGGCTCGGA GAATTATTGC ACAATTTTCC AAAGAGTATG AGACCACCAA CAACCCTGGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966980.1
    CDS416..3961
    Translation

    Target ORF information:

    RefSeq Version XM_014111505.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111505.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGCAA CCCGCCGCAG 
    CCGCTCACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCCTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GCTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAA CCTGTTCCAG CGCACTGTGC GCCTCCGCCG CCGCCTTCCC
    TGCCACCTCC TCGGCCTGGA GGACGATGAG GAAGAGCTAG CTGCAGCTAA CTCGGGTCGG
    GAAAATGCGA CAGCGACTGC CCACTCGAGG TCGTCATGGC GACAGCCAGT GAACGTGTTC
    CTCTCCTCGG GCAGGCCCCC GAGTGTAGAG GAGCTGCTTC GAGAGGCGCA GCTCAATCTC
    CAGAGCCTGT TGCAAGAAGA ATATGAGGAA CAGTACTCAG AGGCCAGACT TCTGGGGCAG
    ACCTTCCGCT CTGCTGATGA GGCCCCCGAG GCCACTCCTA GCCCAAGGCC CCAGGCTGGC
    AGGCGTCTGG AGCTTGTATT GATGCCTACA AAACGGCAGC TGAGCGAGGA TGAGACTACC
    ACCCAGGGTG TGAGGGCCCC TGAGGCCTCC TCGAGCCTGT CTACCACAGC CGACAAGCAA
    GCTGCCTGGA ATAGCCCCTT CCCTCTGCCC ATCCTAGAGG AGAAGCGGTG GCTTCAGCCT
    TGCTCCACGC AATCTGACAT TGTGCCCATC AACATCTCTG GTCATAGCAA CAGGCCTGCA
    GGCAGTGTGG CCCACTCTGC CACCTCAGAC ATCAAGTCCA GTCATTCCAT CCCAGAAGGG
    GTTCATGGAA GAATTGCCAT TGGTCAGGAA GCTCAGTTCC CAAATCTCAC CTCACCAGTA
    CTGAGGAGTC CTTCTAGTGA TTCAGAAGAA CCTCTCCAGG CATGTGGTGG CACAAACCCT
    CCTGGTATGG AGAGCATAGG AATGGTGTAT AGCATCCCCA GTTCTTGCAA TGGACCAACG
    GAATCAACGT TCTCTGCTTC CTGGAAGGGA GATACTTTTA CCTACATGAC TCCAAGTGTC
    ACCAGTCAGA ACAATCAAAT CAGTCAAAAT GGAAGAAATC CTTCCTCTGG GAATGCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTAGTC CCCAAGGAGG CCACTACCTT CTTTGTTACC
    CGAGAGAACC CAACAGGATG CAGTGGGGCA ACTGGCAACT CTGAGCATCC TACTCAACGA
    AGGCAAGTAT CAGAGCGACC CTCCAAGATG GGCCTTCTCA CCAGTGGTAC CTCAAGGCTG
    GAGACAGGCC GGGGGGGTGC CAGCAGGTTC CGGGAGCGGT CACTGTCTGT GCCCATGGAC
    TCTGGCACCA CGGATGTGGA CTATGATGAG GAACAGAAGG CCAGTGAAGC CTGTGCCCTG
    CCTTATGCCA GTACAAGCTC TGAGGGCAGT AACAGTACTG ACAACATTGC CTCCCTTAGC
    ACCCAACAAG AGGCCCAGCC CAGAAGGCAG AGATCCAAGA GTATCTCACT CCGGAAGGCC
    AAGAAGAAGC CTTCCCCACC GATGCGCAGT GTCTCACTGC TCAAAGATGG GCCAGTCCTC
    TTGCCAGAAC ATGGGTCAGC GCTGCCCAAG GACCAGAGGC CCAGGAGTCT CTGCCTCTCC
    TTGGAACACC AAGGGCGTCA GTCATCCCAC CCAGATGCTC AGGGTCACCC AGCTGTGCCA
    ACCGTCAAAG ATCCAGAAGG TGCACAATTC TCCCACCACT GGTATCCTAC TAACTGGAAG
    TCTGGCGACA CCTACCAATC CTTGTCCAGT TCCAGCACGG CCACTGGCAC AACAGTCATC
    GAGTGCACTC AAGTTCAGGG AAGCTCAGAG TCTCTTGCCT CCCCTTCCAC ATCCAGAGCC
    ACGACACCTT CCCAACTCTC TATCGAGGTG GAGGCCAGGG AAGTGTCCTC CCCAGGAAGG
    CCCACTGGGC TGATGTCACC TTCCAGTGGC TACTCCAGCC AATCAGAGAC ACCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC CTGCCCCCTC CAAGTGGCAG TGTCCGGGTG
    CGGCCAGTGG TACCCGAGAG GAAGTCATCA CTCCCCCCGA CATCGCCTAT GGAGAAAATC
    TCCAAGTCCC GGTTGTCCTT TGACCTGCCA CTGACCTCCT CAACCAACCT GGATCTGTCT
    GGGATGAGCA TCTCCATCCG AAGCAAAACC AAGGTGAGCC GGCATCATTC AGATACAAAT
    TTTGGGGCTA AGCTGGCCCT GAAAAAGAGC CCCAACCAGC CATTTATGCC CATGGTCACT
    CAGTCTGACC TACGTTCCAT TCACCTGAGG TCTGTCAGCA AGTCTGAGCC GGAAGATGAC
    ATCGAGAGCC CTGACTATGC TGAGGACGCA GGAGCAGAGG TCTTCGTATT GCTGGAGAGA
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CTTGTGGCCC GAAGTCCTCC CAGCTTGGTC
    CACAAGCCAC CATCTGCCCC TGAGGAGTAC CCACTCACTT CACCGACCTT GGCTGTGACC
    CCCAAGATCT CTGTTCAACA CATGCGGCCA CTCCCCAAAG ACATCTACAC GGTGATGCGG
    AAACCAAAGT CCCCCGGCTT CCCCGAGGGC AGAGGCTCCA GGGAGCCACC AGCGCCCTCT
    TCTCTTGCTT TCACACCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GCACACTGGA GGATGGGGGC CCCAAGGTGA GAGCCCTGCC TGAAAGAATC
    AGCCTCCAGA GCCAGGAAGA AGCAGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTCCCC
    AAAAAACCCA GCGTGCTGTA CCTGCCTCTC ACATCACCCA CAGCACACAT GGAGGTGTTC
    AGGGCCGAAC TGAGGCTGCC CCACAGCCCC ATCATCACCC TGCAGGAAGA CACCAAGTGT
    CCGCCCACCA GTGACCCCCT GAAGGCACCT GGTGTGAGGC CAACTTCACC GCTGCAGGCT
    GAAGGTGAAA GGGAGGCAAG TCCTCTAGGG AGTTCTGTGG AACCGAGCAC TGCAGAAAAA
    AGCATAATCA GTGACAAAAC AGCTGAATGG ATCGCCGAAG ATGATGATGA CGTGTTTGTG
    GCTTCACGCA CAACCGAAGA TTTATTTACT GTGATACACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAAG AGCCTGGGGA GGCCTTTGCT GGTGGCAGCA GATCAAGCTC CCATTCACCA
    ATAAAGAACA CAGCTGATTT TTCCATCAGT GAGTCAGCTG TCTCTGCGGG GTCGAGTGGC
    AGCGCCAACC TGGATGCTGG CAGAAACAAT GATTTCAAGG CCTTGCTGCA AAAGAAGGGA
    AGCAAGGCAA CCCCCAAGTC CCGCCCCTCA GCTGCTGAAC TGCTCAAGAC CACTAATCCG
    CTGGCTCGGA GAATTATTGC ACAATTTTCC AAAGAGTATG AGACCACCAA CAACCCTGGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45523
    Clone ID Related Accession (Same CDS sequence) XM_014111506.1
    Accession Version XM_014111506.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGCAA CCCGCCGCAG 
    CCGCTCACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCCTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GCTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAA CCTGTTCCAG CGCACTGTGC GCCTCCGCCG CCGCCTTCCC
    TGCCACCTCC TCGGCCTGGA GGACGATGAG GAAGAGCTAG AAGAATATGA GGAACAGTAC
    TCAGAGGCCA GACTTCTGGG GCAGACCTTC CGCTCTGCTG ATGAGGCCCC CGAGGCCACT
    CCTAGCCCAA GGCCCCAGGC TGGCAGGCGT CTGGAGCTTG TATTGATGCC TACAAAACGG
    CAGCTGAGCG AGGATGAGAC TACCACCCAG GGTGTGAGGG CCCCTGAGGC CTCCTCGAGC
    CTGTCTACCA CAGCCGACAA GCAAGCTGCC TGGAATAGCC CCTTCCCTCT GCCCATCCTA
    GAGGAGAAGC GGTGGCTTCA GCCTTGCTCC ACGCAATCTG ACATTGTGCC CATCAACATC
    TCTGGGCAGC AGTTTGATAA ACATGCAAGT TTGCGACACT CGTTGTTTAA CACAGAGACA
    GCTGTGAACC CCAAATCCAC CCTGAGGCGG AGGCGGACCA TTATTGGATT CTCTAACTTT
    TCCCAGCGAG ACCAAGGTCA TAGCAACAGG CCTGCAGGCA GTGTGGCCCA CTCTGCCACC
    TCAGACATCA AGTCCAGTCA TTCCATCCCA GAAGGGGTTC ATGGAAGAAT TGCCATTGGT
    CAGGAAGCTC AGTTCCCAAA TCTCACCTCA CCAGTACTGA GGAGTCCTTC TAGTGATTCA
    GAAGAACCTC TCCAGGCATG TGGTGGCACA AACCCTCCTG GTATGGAGAG CATAGGAATG
    GTGTATAGCA TCCCCAGTTC TTGCAATGGA CCAACGGAAT CAACGTTCTC TGCTTCCTGG
    AAGGGAGATA CTTTTACCTA CATGACTCCA AGTGTCACCA GTCAGAACAA TCAAATCAGT
    CAAAATGGAA GAAATCCTTC CTCTGGGAAT GCTTGGGTCT CTCTGCACAC ACTGCCACCT
    CTAGTCCCCA AGGAGGCCAC TACCTTCTTT GTTACCCGAG AGAACCCAAC AGGATGCAGT
    GGGGCAACTG GCAACTCTGA GCATCCTACT CAACGAAGGC AAGTATCAGA GCGACCCTCC
    AAGATGGGCC TTCTCACCAG TGGTACCTCA AGGCTGGAGA CAGGCCGGGG GGGTGCCAGC
    AGGTTCCGGG AGCGGTCACT GTCTGTGCCC ATGGACTCTG GCACCACGGA TGTGGACTAT
    GATGAGGAAC AGAAGGCCAG TGAAGCCTGT GCCCTGCCTT ATGCCAGTAC AAGCTCTGAG
    GGCAGTAACA GTACTGACAA CATTGCCTCC CTTAGCACCC AACAAGAGGC CCAGCCCAGA
    AGGCAGAGAT CCAAGAGTAT CTCACTCCGG AAGGCCAAGA AGAAGCCTTC CCCACCGATG
    CGCAGTGTCT CACTGCTCAA AGATGGGCCA GTCCTCTTGC CAGAACATGG GTCAGCGCTG
    CCCAAGGACC AGAGGCCCAG GAGTCTCTGC CTCTCCTTGG AACACCAAGG GCGTCAGTCA
    TCCCACCCAG ATGCTCAGGG TCACCCAGCT GTGCCAACCG TCAAAGATCC AGAAGGTGCA
    CAATTCTCCC ACCACTGGTA TCCTACTAAC TGGAAGTCTG GCGACACCTA CCAATCCTTG
    TCCAGTTCCA GCACGGCCAC TGGCACAACA GTCATCGAGT GCACTCAAGT TCAGGGAAGC
    TCAGAGTCTC TTGCCTCCCC TTCCACATCC AGAGCCACGA CACCTTCCCA ACTCTCTATC
    GAGGTGGAGG CCAGGGAAGT GTCCTCCCCA GGAAGGCCCA CTGGGCTGAT GTCACCTTCC
    AGTGGCTACT CCAGCCAATC AGAGACACCA ACACCCACTG TCTCCATGTC CTTGACCCTG
    GGCCACCTGC CCCCTCCAAG TGGCAGTGTC CGGGTGCGGC CAGTGGTACC CGAGAGGAAG
    TCATCACTCC CCCCGACATC GCCTATGGAG AAAATCTCCA AGTCCCGGTT GTCCTTTGAC
    CTGCCACTGA CCTCCTCAAC CAACCTGGAT CTGTCTGGGA TGAGCATCTC CATCCGAAGC
    AAAACCAAGG TGAGCCGGCA TCATTCAGAT ACAAATTTTG GGGCTAAGCT GGCCCTGAAA
    AAGAGCCCCA ACCAGCCATT TATGCCCATG GTCACTCAGT CTGACCTACG TTCCATTCAC
    CTGAGGTCTG TCAGCAAGTC TGAGCCGGAA GATGACATCG AGAGCCCTGA CTATGCTGAG
    GACGCAGGAG CAGAGGTCTT CGTATTGCTG GAGAGAAAGA TGAAACCTCC CATAGCCGAG
    AAGCCCCTTG TGGCCCGAAG TCCTCCCAGC TTGGTCCACA AGCCACCATC TGCCCCTGAG
    GAGTACCCAC TCACTTCACC GACCTTGGCT GTGACCCCCA AGATCTCTGT TCAACACATG
    CGGCCACTCC CCAAAGACAT CTACACGGTG ATGCGGAAAC CAAAGTCCCC CGGCTTCCCC
    GAGGGCAGAG GCTCCAGGGA GCCACCAGCG CCCTCTTCTC TTGCTTTCAC ACCTTTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGGCAC ACTGGAGGAT
    GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA AGAATCAGCC TCCAGAGCCA GGAAGAAGCA
    GAGAAAAAGA AAGGCAAGAT TCCACCTCCT GTCCCCAAAA AACCCAGCGT GCTGTACCTG
    CCTCTCACAT CACCCACAGC ACACATGGAG GTGTTCAGGG CCGAACTGAG GCTGCCCCAC
    AGCCCCATCA TCACCCTGCA GGAAGACACC AAGTGTCCGC CCACCAGTGA CCCCCTGAAG
    GCACCTGGTG TGAGGCCAAC TTCACCGCTG CAGGCTGAAG GTGAAAGGGA GGCAAGTCCT
    CTAGGGAGTT CTGTGGAACC GAGCACTGCA GAAAAAAGCA TAATCAGTGA CAAAACAGCT
    GAATGGATCG CCGAAGATGA TGATGACGTG TTTGTGGCTT CACGCACAAC CGAAGATTTA
    TTTACTGTGA TACACAGGTC CAAAAGAAAG CTGCTTGGCT GGAAAGAGCC TGGGGAGGCC
    TTTGCTGGTG GCAGCAGATC AAGCTCCCAT TCACCAATAA AGAACACAGC TGATTTTTCC
    ATCAGTGAGT CAGCTGTCTC TGCGGGGTCG AGTGGCAGCG CCAACCTGGA TGCTGGCAGA
    AACAATGATT TCAAGGCCTT GCTGCAAAAG AAGGGAAGCA AGGCAACCCC CAAGTCCCGC
    CCCTCAGCTG CTGAACTGCT CAAGACCACT AATCCGCTGG CTCGGAGAAT TATTGCACAA
    TTTTCCAAAG AGTATGAGAC CACCAACAAC CCTGGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966981.1
    CDS415..3936
    Translation

    Target ORF information:

    RefSeq Version XM_014111506.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111506.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGCAA CCCGCCGCAG 
    CCGCTCACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCCTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GCTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAA CCTGTTCCAG CGCACTGTGC GCCTCCGCCG CCGCCTTCCC
    TGCCACCTCC TCGGCCTGGA GGACGATGAG GAAGAGCTAG AAGAATATGA GGAACAGTAC
    TCAGAGGCCA GACTTCTGGG GCAGACCTTC CGCTCTGCTG ATGAGGCCCC CGAGGCCACT
    CCTAGCCCAA GGCCCCAGGC TGGCAGGCGT CTGGAGCTTG TATTGATGCC TACAAAACGG
    CAGCTGAGCG AGGATGAGAC TACCACCCAG GGTGTGAGGG CCCCTGAGGC CTCCTCGAGC
    CTGTCTACCA CAGCCGACAA GCAAGCTGCC TGGAATAGCC CCTTCCCTCT GCCCATCCTA
    GAGGAGAAGC GGTGGCTTCA GCCTTGCTCC ACGCAATCTG ACATTGTGCC CATCAACATC
    TCTGGGCAGC AGTTTGATAA ACATGCAAGT TTGCGACACT CGTTGTTTAA CACAGAGACA
    GCTGTGAACC CCAAATCCAC CCTGAGGCGG AGGCGGACCA TTATTGGATT CTCTAACTTT
    TCCCAGCGAG ACCAAGGTCA TAGCAACAGG CCTGCAGGCA GTGTGGCCCA CTCTGCCACC
    TCAGACATCA AGTCCAGTCA TTCCATCCCA GAAGGGGTTC ATGGAAGAAT TGCCATTGGT
    CAGGAAGCTC AGTTCCCAAA TCTCACCTCA CCAGTACTGA GGAGTCCTTC TAGTGATTCA
    GAAGAACCTC TCCAGGCATG TGGTGGCACA AACCCTCCTG GTATGGAGAG CATAGGAATG
    GTGTATAGCA TCCCCAGTTC TTGCAATGGA CCAACGGAAT CAACGTTCTC TGCTTCCTGG
    AAGGGAGATA CTTTTACCTA CATGACTCCA AGTGTCACCA GTCAGAACAA TCAAATCAGT
    CAAAATGGAA GAAATCCTTC CTCTGGGAAT GCTTGGGTCT CTCTGCACAC ACTGCCACCT
    CTAGTCCCCA AGGAGGCCAC TACCTTCTTT GTTACCCGAG AGAACCCAAC AGGATGCAGT
    GGGGCAACTG GCAACTCTGA GCATCCTACT CAACGAAGGC AAGTATCAGA GCGACCCTCC
    AAGATGGGCC TTCTCACCAG TGGTACCTCA AGGCTGGAGA CAGGCCGGGG GGGTGCCAGC
    AGGTTCCGGG AGCGGTCACT GTCTGTGCCC ATGGACTCTG GCACCACGGA TGTGGACTAT
    GATGAGGAAC AGAAGGCCAG TGAAGCCTGT GCCCTGCCTT ATGCCAGTAC AAGCTCTGAG
    GGCAGTAACA GTACTGACAA CATTGCCTCC CTTAGCACCC AACAAGAGGC CCAGCCCAGA
    AGGCAGAGAT CCAAGAGTAT CTCACTCCGG AAGGCCAAGA AGAAGCCTTC CCCACCGATG
    CGCAGTGTCT CACTGCTCAA AGATGGGCCA GTCCTCTTGC CAGAACATGG GTCAGCGCTG
    CCCAAGGACC AGAGGCCCAG GAGTCTCTGC CTCTCCTTGG AACACCAAGG GCGTCAGTCA
    TCCCACCCAG ATGCTCAGGG TCACCCAGCT GTGCCAACCG TCAAAGATCC AGAAGGTGCA
    CAATTCTCCC ACCACTGGTA TCCTACTAAC TGGAAGTCTG GCGACACCTA CCAATCCTTG
    TCCAGTTCCA GCACGGCCAC TGGCACAACA GTCATCGAGT GCACTCAAGT TCAGGGAAGC
    TCAGAGTCTC TTGCCTCCCC TTCCACATCC AGAGCCACGA CACCTTCCCA ACTCTCTATC
    GAGGTGGAGG CCAGGGAAGT GTCCTCCCCA GGAAGGCCCA CTGGGCTGAT GTCACCTTCC
    AGTGGCTACT CCAGCCAATC AGAGACACCA ACACCCACTG TCTCCATGTC CTTGACCCTG
    GGCCACCTGC CCCCTCCAAG TGGCAGTGTC CGGGTGCGGC CAGTGGTACC CGAGAGGAAG
    TCATCACTCC CCCCGACATC GCCTATGGAG AAAATCTCCA AGTCCCGGTT GTCCTTTGAC
    CTGCCACTGA CCTCCTCAAC CAACCTGGAT CTGTCTGGGA TGAGCATCTC CATCCGAAGC
    AAAACCAAGG TGAGCCGGCA TCATTCAGAT ACAAATTTTG GGGCTAAGCT GGCCCTGAAA
    AAGAGCCCCA ACCAGCCATT TATGCCCATG GTCACTCAGT CTGACCTACG TTCCATTCAC
    CTGAGGTCTG TCAGCAAGTC TGAGCCGGAA GATGACATCG AGAGCCCTGA CTATGCTGAG
    GACGCAGGAG CAGAGGTCTT CGTATTGCTG GAGAGAAAGA TGAAACCTCC CATAGCCGAG
    AAGCCCCTTG TGGCCCGAAG TCCTCCCAGC TTGGTCCACA AGCCACCATC TGCCCCTGAG
    GAGTACCCAC TCACTTCACC GACCTTGGCT GTGACCCCCA AGATCTCTGT TCAACACATG
    CGGCCACTCC CCAAAGACAT CTACACGGTG ATGCGGAAAC CAAAGTCCCC CGGCTTCCCC
    GAGGGCAGAG GCTCCAGGGA GCCACCAGCG CCCTCTTCTC TTGCTTTCAC ACCTTTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGGCAC ACTGGAGGAT
    GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA AGAATCAGCC TCCAGAGCCA GGAAGAAGCA
    GAGAAAAAGA AAGGCAAGAT TCCACCTCCT GTCCCCAAAA AACCCAGCGT GCTGTACCTG
    CCTCTCACAT CACCCACAGC ACACATGGAG GTGTTCAGGG CCGAACTGAG GCTGCCCCAC
    AGCCCCATCA TCACCCTGCA GGAAGACACC AAGTGTCCGC CCACCAGTGA CCCCCTGAAG
    GCACCTGGTG TGAGGCCAAC TTCACCGCTG CAGGCTGAAG GTGAAAGGGA GGCAAGTCCT
    CTAGGGAGTT CTGTGGAACC GAGCACTGCA GAAAAAAGCA TAATCAGTGA CAAAACAGCT
    GAATGGATCG CCGAAGATGA TGATGACGTG TTTGTGGCTT CACGCACAAC CGAAGATTTA
    TTTACTGTGA TACACAGGTC CAAAAGAAAG CTGCTTGGCT GGAAAGAGCC TGGGGAGGCC
    TTTGCTGGTG GCAGCAGATC AAGCTCCCAT TCACCAATAA AGAACACAGC TGATTTTTCC
    ATCAGTGAGT CAGCTGTCTC TGCGGGGTCG AGTGGCAGCG CCAACCTGGA TGCTGGCAGA
    AACAATGATT TCAAGGCCTT GCTGCAAAAG AAGGGAAGCA AGGCAACCCC CAAGTCCCGC
    CCCTCAGCTG CTGAACTGCT CAAGACCACT AATCCGCTGG CTCGGAGAAT TATTGCACAA
    TTTTCCAAAG AGTATGAGAC CACCAACAAC CCTGGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45524
    Clone ID Related Accession (Same CDS sequence) XM_014111507.1
    Accession Version XM_014111507.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGGTCCA GTCGATCTGC AGTCAGGAGA CCCACTGCAG CTAACTCGGG TCGGGAAAAT 
    GCGACAGCGA CTGCCCACTC GAGGTCGTCA TGGCGACAGC CAGTGAACGT GTTCCTCTCC
    TCGGGCAGGC CCCCGAGTGT AGAGGAGCTG CTTCGAGAGG CGCAGCTCAA TCTCCAGAGC
    CTGTTGCAAG AAGAATATGA GGAACAGTAC TCAGAGGCCA GACTTCTGGG GCAGACCTTC
    CGCTCTGCTG ATGAGGCCCC CGAGGCCACT CCTAGCCCAA GGCCCCAGGC TGGCAGGCGT
    CTGGAGCTTG TATTGATGCC TACAAAACGG CAGCTGAGCG AGGATGAGAC TACCACCCAG
    GGTGTGAGGG CCCCTGAGGC CTCCTCGAGC CTGTCTACCA CAGCCGACAA GCAAGCTGCC
    TGGAATAGCC CCTTCCCTCT GCCCATCCTA GAGGAGAAGC GGTGGCTTCA GCCTTGCTCC
    ACGCAATCTG ACATTGTGCC CATCAACATC TCTGGGCAGC AGTTTGATAA ACATGCAAGT
    TTGCGACACT CGTTGTTTAA CACAGAGACA GCTGTGAACC CCAAATCCAC CCTGAGGCGG
    AGGCGGACCA TTATTGGATT CTCTAACTTT TCCCAGCGAG ACCAAGGTCA TAGCAACAGG
    CCTGCAGGCA GTGTGGCCCA CTCTGCCACC TCAGACATCA AGTCCAGTCA TTCCATCCCA
    GAAGGGGTTC ATGGAAGAAT TGCCATTGGT CAGGAAGCTC AGTTCCCAAA TCTCACCTCA
    CCAGTACTGA GGAGTCCTTC TAGTGATTCA GAAGAACCTC TCCAGGCATG TGGTGGCACA
    AACCCTCCTG GTATGGAGAG CATAGGAATG GTGTATAGCA TCCCCAGTTC TTGCAATGGA
    CCAACGGAAT CAACGTTCTC TGCTTCCTGG AAGGGAGATA CTTTTACCTA CATGACTCCA
    AGTGTCACCA GTCAGAACAA TCAAATCAGT CAAAATGGAA GAAATCCTTC CTCTGGGAAT
    GCTTGGGTCT CTCTGCACAC ACTGCCACCT CTAGTCCCCA AGGAGGCCAC TACCTTCTTT
    GTTACCCGAG AGAACCCAAC AGGATGCAGT GGGGCAACTG GCAACTCTGA GCATCCTACT
    CAACGAAGGC AAGTATCAGA GCGACCCTCC AAGATGGGCC TTCTCACCAG TGGTACCTCA
    AGGCTGGAGA CAGGCCGGGG GGGTGCCAGC AGGTTCCGGG AGCGGTCACT GTCTGTGCCC
    ATGGACTCTG GCACCACGGA TGTGGACTAT GATGAGGAAC AGAAGGCCAG TGAAGCCTGT
    GCCCTGCCTT ATGCCAGTAC AAGCTCTGAG GGCAGTAACA GTACTGACAA CATTGCCTCC
    CTTAGCACCC AACAAGAGGC CCAGCCCAGA AGGCAGAGAT CCAAGAGTAT CTCACTCCGG
    AAGGCCAAGA AGAAGCCTTC CCCACCGATG CGCAGTGTCT CACTGCTCAA AGATGGGCCA
    GTCCTCTTGC CAGAACATGG GTCAGCGCTG CCCAAGGACC AGAGGCCCAG GAGTCTCTGC
    CTCTCCTTGG AACACCAAGG GCGTCAGTCA TCCCACCCAG ATGCTCAGGG TCACCCAGCT
    GTGCCAACCG TCAAAGATCC AGAAGGTGCA CAATTCTCCC ACCACTGGTA TCCTACTAAC
    TGGAAGTCTG GCGACACCTA CCAATCCTTG TCCAGTTCCA GCACGGCCAC TGGCACAACA
    GTCATCGAGT GCACTCAAGT TCAGGGAAGC TCAGAGTCTC TTGCCTCCCC TTCCACATCC
    AGAGCCACGA CACCTTCCCA ACTCTCTATC GAGGTGGAGG CCAGGGAAGT GTCCTCCCCA
    GGAAGGCCCA CTGGGCTGAT GTCACCTTCC AGTGGCTACT CCAGCCAATC AGAGACACCA
    ACACCCACTG TCTCCATGTC CTTGACCCTG GGCCACCTGC CCCCTCCAAG TGGCAGTGTC
    CGGGTGCGGC CAGTGGTACC CGAGAGGAAG TCATCACTCC CCCCGACATC GCCTATGGAG
    AAAATCTCCA AGTCCCGGTT GTCCTTTGAC CTGCCACTGA CCTCCTCAAC CAACCTGGAT
    CTGTCTGGGA TGAGCATCTC CATCCGAAGC AAAACCAAGG TGAGCCGGCA TCATTCAGAT
    ACAAATTTTG GGGCTAAGCT GGCCCTGAAA AAGAGCCCCA ACCAGCCATT TATGCCCATG
    GTCACTCAGT CTGACCTACG TTCCATTCAC CTGAGGTCTG TCAGCAAGTC TGAGCCGGAA
    GATGACATCG AGAGCCCTGA CTATGCTGAG GACGCAGGAG CAGAGGTCTT CGTATTGCTG
    GAGAGAAAGA TGAAACCTCC CATAGCCGAG AAGCCCCTTG TGGCCCGAAG TCCTCCCAGC
    TTGGTCCACA AGCCACCATC TGCCCCTGAG GAGTACCCAC TCACTTCACC GACCTTGGCT
    GTGACCCCCA AGATCTCTGT TCAACACATG CGGCCACTCC CCAAAGACAT CTACACGGTG
    ATGCGGAAAC CAAAGTCCCC CGGCTTCCCC GAGGGCAGAG GCTCCAGGGA GCCACCAGCG
    CCCTCTTCTC TTGCTTTCAC ACCTTTTGCC AGTTCCTCTG GTGCTTTCTT CTCAGGAACA
    CAGCAACCTC CCCAGGGCAC ACTGGAGGAT GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA
    AGAATCAGCC TCCAGAGCCA GGAAGAAGCA GAGAAAAAGA AAGGCAAGAT TCCACCTCCT
    GTCCCCAAAA AACCCAGCGT GCTGTACCTG CCTCTCACAT CACCCACAGC ACACATGGAG
    GTGTTCAGGG CCGAACTGAG GCTGCCCCAC AGCCCCATCA TCACCCTGCA GGAAGACACC
    AAGTGTCCGC CCACCAGTGA CCCCCTGAAG GCACCTGGTG TGAGGCCAAC TTCACCGCTG
    CAGGCTGAAG GTGAAAGGGA GGCAAGTCCT CTAGGGAGTT CTGTGGAACC GAGCACTGCA
    GAAAAAAGCA TAATCAGTGA CAAAACAGCT GAATGGATCG CCGAAGATGA TGATGACGTG
    TTTGTGGCTT CACGCACAAC CGAAGATTTA TTTACTGTGA TACACAGGTC CAAAAGAAAG
    CTGCTTGGCT GGAAAGAGCC TGGGGAGGCC TTTGCTGGTG GCAGCAGATC AAGCTCCCAT
    TCACCAATAA AGAACACAGC TGATTTTTCC ATCAGTGAGT CAGCTGTCTC TGCGGGGTCG
    AGTGGCAGCG CCAACCTGGA TGCTGGCAGA AACAATGATT TCAAGGCCTT GCTGCAAAAG
    AAGGGAAGCA AGGCAACCCC CAAGTCCCGC CCCTCAGCTG CTGAACTGCT CAAGACCACT
    AATCCGCTGG CTCGGAGAAT TATTGCACAA TTTTCCAAAG AGTATGAGAC CACCAACAAC
    CCTGGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966982.1
    CDS168..3599
    Translation

    Target ORF information:

    RefSeq Version XM_014111507.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111507.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGGTCCA GTCGATCTGC AGTCAGGAGA CCCACTGCAG CTAACTCGGG TCGGGAAAAT 
    GCGACAGCGA CTGCCCACTC GAGGTCGTCA TGGCGACAGC CAGTGAACGT GTTCCTCTCC
    TCGGGCAGGC CCCCGAGTGT AGAGGAGCTG CTTCGAGAGG CGCAGCTCAA TCTCCAGAGC
    CTGTTGCAAG AAGAATATGA GGAACAGTAC TCAGAGGCCA GACTTCTGGG GCAGACCTTC
    CGCTCTGCTG ATGAGGCCCC CGAGGCCACT CCTAGCCCAA GGCCCCAGGC TGGCAGGCGT
    CTGGAGCTTG TATTGATGCC TACAAAACGG CAGCTGAGCG AGGATGAGAC TACCACCCAG
    GGTGTGAGGG CCCCTGAGGC CTCCTCGAGC CTGTCTACCA CAGCCGACAA GCAAGCTGCC
    TGGAATAGCC CCTTCCCTCT GCCCATCCTA GAGGAGAAGC GGTGGCTTCA GCCTTGCTCC
    ACGCAATCTG ACATTGTGCC CATCAACATC TCTGGGCAGC AGTTTGATAA ACATGCAAGT
    TTGCGACACT CGTTGTTTAA CACAGAGACA GCTGTGAACC CCAAATCCAC CCTGAGGCGG
    AGGCGGACCA TTATTGGATT CTCTAACTTT TCCCAGCGAG ACCAAGGTCA TAGCAACAGG
    CCTGCAGGCA GTGTGGCCCA CTCTGCCACC TCAGACATCA AGTCCAGTCA TTCCATCCCA
    GAAGGGGTTC ATGGAAGAAT TGCCATTGGT CAGGAAGCTC AGTTCCCAAA TCTCACCTCA
    CCAGTACTGA GGAGTCCTTC TAGTGATTCA GAAGAACCTC TCCAGGCATG TGGTGGCACA
    AACCCTCCTG GTATGGAGAG CATAGGAATG GTGTATAGCA TCCCCAGTTC TTGCAATGGA
    CCAACGGAAT CAACGTTCTC TGCTTCCTGG AAGGGAGATA CTTTTACCTA CATGACTCCA
    AGTGTCACCA GTCAGAACAA TCAAATCAGT CAAAATGGAA GAAATCCTTC CTCTGGGAAT
    GCTTGGGTCT CTCTGCACAC ACTGCCACCT CTAGTCCCCA AGGAGGCCAC TACCTTCTTT
    GTTACCCGAG AGAACCCAAC AGGATGCAGT GGGGCAACTG GCAACTCTGA GCATCCTACT
    CAACGAAGGC AAGTATCAGA GCGACCCTCC AAGATGGGCC TTCTCACCAG TGGTACCTCA
    AGGCTGGAGA CAGGCCGGGG GGGTGCCAGC AGGTTCCGGG AGCGGTCACT GTCTGTGCCC
    ATGGACTCTG GCACCACGGA TGTGGACTAT GATGAGGAAC AGAAGGCCAG TGAAGCCTGT
    GCCCTGCCTT ATGCCAGTAC AAGCTCTGAG GGCAGTAACA GTACTGACAA CATTGCCTCC
    CTTAGCACCC AACAAGAGGC CCAGCCCAGA AGGCAGAGAT CCAAGAGTAT CTCACTCCGG
    AAGGCCAAGA AGAAGCCTTC CCCACCGATG CGCAGTGTCT CACTGCTCAA AGATGGGCCA
    GTCCTCTTGC CAGAACATGG GTCAGCGCTG CCCAAGGACC AGAGGCCCAG GAGTCTCTGC
    CTCTCCTTGG AACACCAAGG GCGTCAGTCA TCCCACCCAG ATGCTCAGGG TCACCCAGCT
    GTGCCAACCG TCAAAGATCC AGAAGGTGCA CAATTCTCCC ACCACTGGTA TCCTACTAAC
    TGGAAGTCTG GCGACACCTA CCAATCCTTG TCCAGTTCCA GCACGGCCAC TGGCACAACA
    GTCATCGAGT GCACTCAAGT TCAGGGAAGC TCAGAGTCTC TTGCCTCCCC TTCCACATCC
    AGAGCCACGA CACCTTCCCA ACTCTCTATC GAGGTGGAGG CCAGGGAAGT GTCCTCCCCA
    GGAAGGCCCA CTGGGCTGAT GTCACCTTCC AGTGGCTACT CCAGCCAATC AGAGACACCA
    ACACCCACTG TCTCCATGTC CTTGACCCTG GGCCACCTGC CCCCTCCAAG TGGCAGTGTC
    CGGGTGCGGC CAGTGGTACC CGAGAGGAAG TCATCACTCC CCCCGACATC GCCTATGGAG
    AAAATCTCCA AGTCCCGGTT GTCCTTTGAC CTGCCACTGA CCTCCTCAAC CAACCTGGAT
    CTGTCTGGGA TGAGCATCTC CATCCGAAGC AAAACCAAGG TGAGCCGGCA TCATTCAGAT
    ACAAATTTTG GGGCTAAGCT GGCCCTGAAA AAGAGCCCCA ACCAGCCATT TATGCCCATG
    GTCACTCAGT CTGACCTACG TTCCATTCAC CTGAGGTCTG TCAGCAAGTC TGAGCCGGAA
    GATGACATCG AGAGCCCTGA CTATGCTGAG GACGCAGGAG CAGAGGTCTT CGTATTGCTG
    GAGAGAAAGA TGAAACCTCC CATAGCCGAG AAGCCCCTTG TGGCCCGAAG TCCTCCCAGC
    TTGGTCCACA AGCCACCATC TGCCCCTGAG GAGTACCCAC TCACTTCACC GACCTTGGCT
    GTGACCCCCA AGATCTCTGT TCAACACATG CGGCCACTCC CCAAAGACAT CTACACGGTG
    ATGCGGAAAC CAAAGTCCCC CGGCTTCCCC GAGGGCAGAG GCTCCAGGGA GCCACCAGCG
    CCCTCTTCTC TTGCTTTCAC ACCTTTTGCC AGTTCCTCTG GTGCTTTCTT CTCAGGAACA
    CAGCAACCTC CCCAGGGCAC ACTGGAGGAT GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA
    AGAATCAGCC TCCAGAGCCA GGAAGAAGCA GAGAAAAAGA AAGGCAAGAT TCCACCTCCT
    GTCCCCAAAA AACCCAGCGT GCTGTACCTG CCTCTCACAT CACCCACAGC ACACATGGAG
    GTGTTCAGGG CCGAACTGAG GCTGCCCCAC AGCCCCATCA TCACCCTGCA GGAAGACACC
    AAGTGTCCGC CCACCAGTGA CCCCCTGAAG GCACCTGGTG TGAGGCCAAC TTCACCGCTG
    CAGGCTGAAG GTGAAAGGGA GGCAAGTCCT CTAGGGAGTT CTGTGGAACC GAGCACTGCA
    GAAAAAAGCA TAATCAGTGA CAAAACAGCT GAATGGATCG CCGAAGATGA TGATGACGTG
    TTTGTGGCTT CACGCACAAC CGAAGATTTA TTTACTGTGA TACACAGGTC CAAAAGAAAG
    CTGCTTGGCT GGAAAGAGCC TGGGGAGGCC TTTGCTGGTG GCAGCAGATC AAGCTCCCAT
    TCACCAATAA AGAACACAGC TGATTTTTCC ATCAGTGAGT CAGCTGTCTC TGCGGGGTCG
    AGTGGCAGCG CCAACCTGGA TGCTGGCAGA AACAATGATT TCAAGGCCTT GCTGCAAAAG
    AAGGGAAGCA AGGCAACCCC CAAGTCCCGC CCCTCAGCTG CTGAACTGCT CAAGACCACT
    AATCCGCTGG CTCGGAGAAT TATTGCACAA TTTTCCAAAG AGTATGAGAC CACCAACAAC
    CCTGGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45525
    Clone ID Related Accession (Same CDS sequence) XM_014111508.1
    Accession Version XM_014111508.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAACAC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGA GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCAGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCCGAGGC CACTCCTAGC CCAAGGCCCC AGGCTGGCAG GCGTCTGGAG
    CTTGTATTGA TGCCTACAAA ACGGCAGCTG AGCGAGGATG AGACTACCAC CCAGGGTGTG
    AGGGCCCCTG AGGCCTCCTC GAGCCTGTCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTCC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGCCTTG CTCCACGCAA
    TCTGACATTG TGCCCATCAA CATCTCTGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCTGTG AACCCCAAAT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTTTTCCCAG CGAGACCAAG GTCATAGCAA CAGGCCTGCA
    GGCAGTGTGG CCCACTCTGC CACCTCAGAC ATCAAGTCCA GTCATTCCAT CCCAGAAGGG
    GTTCATGGAA GAATTGCCAT TGGTCAGGAA GCTCAGTTCC CAAATCTCAC CTCACCAGTA
    CTGAGGAGTC CTTCTAGTGA TTCAGAAGAA CCTCTCCAGG CATGTGGTGG CACAAACCCT
    CCTGGTATGG AGAGCATAGG AATGGTGTAT AGCATCCCCA GTTCTTGCAA TGGACCAACG
    GAATCAACGT TCTCTGCTTC CTGGAAGGGA GATACTTTTA CCTACATGAC TCCAAGTGTC
    ACCAGTCAGA ACAATCAAAT CAGTCAAAAT GGAAGAAATC CTTCCTCTGG GAATGCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTAGTC CCCAAGGAGG CCACTACCTT CTTTGTTACC
    CGAGAGAACC CAACAGGATG CAGTGGGGCA ACTGGCAACT CTGAGCATCC TACTCAACGA
    AGGCAAGTAT CAGAGCGACC CTCCAAGATG GGCCTTCTCA CCAGTGGTAC CTCAAGGCTG
    GAGACAGGCC GGGGGGGTGC CAGCAGGTTC CGGGAGCGGT CACTGTCTGT GCCCATGGAC
    TCTGGCACCA CGGATGTGGA CTATGATGAG GAACAGAAGG CCAGTGAAGC CTGTGCCCTG
    CCTTATGCCA GTACAAGCTC TGAGGGCAGT AACAGTACTG ACAACATTGC CTCCCTTAGC
    ACCCAACAAG AGGCCCAGCC CAGAAGGCAG AGATCCAAGA GTATCTCACT CCGGAAGGCC
    AAGAAGAAGC CTTCCCCACC GATGCGCAGT GTCTCACTGC TCAAAGATGG GCCAGTCCTC
    TTGCCAGAAC ATGGGTCAGC GCTGCCCAAG GACCAGAGGC CCAGGAGTCT CTGCCTCTCC
    TTGGAACACC AAGGGCGTCA GTCATCCCAC CCAGATGCTC AGGGTCACCC AGCTGTGCCA
    ACCGTCAAAG ATCCAGAAGG TGCACAATTC TCCCACCACT GGTATCCTAC TAACTGGAAG
    TCTGGCGACA CCTACCAATC CTTGTCCAGT TCCAGCACGG CCACTGGCAC AACAGTCATC
    GAGTGCACTC AAGTTCAGGG AAGCTCAGAG TCTCTTGCCT CCCCTTCCAC ATCCAGAGCC
    ACGACACCTT CCCAACTCTC TATCGAGGTG GAGGCCAGGG AAGTGTCCTC CCCAGGAAGG
    CCCACTGGGC TGATGTCACC TTCCAGTGGC TACTCCAGCC AATCAGAGAC ACCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC CTGCCCCCTC CAAGTGGCAG TGTCCGGGTG
    CGGCCAGTGG TACCCGAGAG GAAGTCATCA CTCCCCCCGA CATCGCCTAT GGAGAAAATC
    TCCAAGTCCC GGTTGTCCTT TGACCTGCCA CTGACCTCCT CAACCAACCT GGATCTGTCT
    GGGATGAGCA TCTCCATCCG AAGCAAAACC AAGGTGAGCC GGCATCATTC AGATACAAAT
    TTTGGGGCTA AGCTGGCCCT GAAAAAGAGC CCCAACCAGC CATTTATGCC CATGGTCACT
    CAGTCTGACC TACGTTCCAT TCACCTGAGG TCTGTCAGCA AGTCTGAGCC GGAAGATGAC
    ATCGAGAGCC CTGACTATGC TGAGGACGCA GGAGCAGAGG TCTTCGTATT GCTGGAGAGA
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CTTGTGGCCC GAAGTCCTCC CAGCTTGGTC
    CACAAGCCAC CATCTGCCCC TGAGGAGTAC CCACTCACTT CACCGACCTT GGCTGTGACC
    CCCAAGATCT CTGTTCAACA CATGCGGCCA CTCCCCAAAG ACATCTACAC GGTGATGCGG
    AAACCAAAGT CCCCCGGCTT CCCCGAGGGC AGAGGCTCCA GGGAGCCACC AGCGCCCTCT
    TCTCTTGCTT TCACACCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GCACACTGGA GGATGGGGGC CCCAAGGTGA GAGCCCTGCC TGAAAGAATC
    AGCCTCCAGA GCCAGGAAGA AGCAGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTCCCC
    AAAAAACCCA GCGTGCTGTA CCTGCCTCTC ACATCACCCA CAGCACACAT GGAGGTGTTC
    AGGGCCGAAC TGAGGCTGCC CCACAGCCCC ATCATCACCC TGCAGGAAGA CACCAAGTGT
    CCGCCCACCA GTGACCCCCT GAAGGCACCT GGTGTGAGGC CAACTTCACC GCTGCAGGCT
    GAAGGTGAAA GGGAGGCAAG TCCTCTAGGG AGTTCTGTGG AACCGAGCAC TGCAGAAAAA
    AGCATAATCA GTGACAAAAC AGCTGAATGG ATCGCCGAAG ATGATGATGA CGTGTTTGTG
    GCTTCACGCA CAACCGAAGA TTTATTTACT GTGATACACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAAG AGCCTGGGGA GGCCTTTGCT GGTGGCAGCA GATCAAGCTC CCATTCACCA
    ATAAAGAACA CAGCTGATTT TTCCATCAGT GAGTCAGCTG TCTCTGCGGG GTCGAGTGGC
    AGCGCCAACC TGGATGCTGG CAGAAACAAT GATTTCAAGG CCTTGCTGCA AAAGAAGGGA
    AGCAAGGCAA CCCCCAAGTC CCGCCCCTCA GCTGCTGAAC TGCTCAAGAC CACTAATCCG
    CTGGCTCGGA GAATTATTGC ACAATTTTCC AAAGAGTATG AGACCACCAA CAACCCTGGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966983.1
    CDS41..3466
    Translation

    Target ORF information:

    RefSeq Version XM_014111508.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111508.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCAACAC AGCCTAGAGC ATGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA GCTGCTTCGA GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTACTCAGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    GCTGATGAGG CCCCCGAGGC CACTCCTAGC CCAAGGCCCC AGGCTGGCAG GCGTCTGGAG
    CTTGTATTGA TGCCTACAAA ACGGCAGCTG AGCGAGGATG AGACTACCAC CCAGGGTGTG
    AGGGCCCCTG AGGCCTCCTC GAGCCTGTCT ACCACAGCCG ACAAGCAAGC TGCCTGGAAT
    AGCCCCTTCC CTCTGCCCAT CCTAGAGGAG AAGCGGTGGC TTCAGCCTTG CTCCACGCAA
    TCTGACATTG TGCCCATCAA CATCTCTGGG CAGCAGTTTG ATAAACATGC AAGTTTGCGA
    CACTCGTTGT TTAACACAGA GACAGCTGTG AACCCCAAAT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTTTTCCCAG CGAGACCAAG GTCATAGCAA CAGGCCTGCA
    GGCAGTGTGG CCCACTCTGC CACCTCAGAC ATCAAGTCCA GTCATTCCAT CCCAGAAGGG
    GTTCATGGAA GAATTGCCAT TGGTCAGGAA GCTCAGTTCC CAAATCTCAC CTCACCAGTA
    CTGAGGAGTC CTTCTAGTGA TTCAGAAGAA CCTCTCCAGG CATGTGGTGG CACAAACCCT
    CCTGGTATGG AGAGCATAGG AATGGTGTAT AGCATCCCCA GTTCTTGCAA TGGACCAACG
    GAATCAACGT TCTCTGCTTC CTGGAAGGGA GATACTTTTA CCTACATGAC TCCAAGTGTC
    ACCAGTCAGA ACAATCAAAT CAGTCAAAAT GGAAGAAATC CTTCCTCTGG GAATGCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTAGTC CCCAAGGAGG CCACTACCTT CTTTGTTACC
    CGAGAGAACC CAACAGGATG CAGTGGGGCA ACTGGCAACT CTGAGCATCC TACTCAACGA
    AGGCAAGTAT CAGAGCGACC CTCCAAGATG GGCCTTCTCA CCAGTGGTAC CTCAAGGCTG
    GAGACAGGCC GGGGGGGTGC CAGCAGGTTC CGGGAGCGGT CACTGTCTGT GCCCATGGAC
    TCTGGCACCA CGGATGTGGA CTATGATGAG GAACAGAAGG CCAGTGAAGC CTGTGCCCTG
    CCTTATGCCA GTACAAGCTC TGAGGGCAGT AACAGTACTG ACAACATTGC CTCCCTTAGC
    ACCCAACAAG AGGCCCAGCC CAGAAGGCAG AGATCCAAGA GTATCTCACT CCGGAAGGCC
    AAGAAGAAGC CTTCCCCACC GATGCGCAGT GTCTCACTGC TCAAAGATGG GCCAGTCCTC
    TTGCCAGAAC ATGGGTCAGC GCTGCCCAAG GACCAGAGGC CCAGGAGTCT CTGCCTCTCC
    TTGGAACACC AAGGGCGTCA GTCATCCCAC CCAGATGCTC AGGGTCACCC AGCTGTGCCA
    ACCGTCAAAG ATCCAGAAGG TGCACAATTC TCCCACCACT GGTATCCTAC TAACTGGAAG
    TCTGGCGACA CCTACCAATC CTTGTCCAGT TCCAGCACGG CCACTGGCAC AACAGTCATC
    GAGTGCACTC AAGTTCAGGG AAGCTCAGAG TCTCTTGCCT CCCCTTCCAC ATCCAGAGCC
    ACGACACCTT CCCAACTCTC TATCGAGGTG GAGGCCAGGG AAGTGTCCTC CCCAGGAAGG
    CCCACTGGGC TGATGTCACC TTCCAGTGGC TACTCCAGCC AATCAGAGAC ACCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC CTGCCCCCTC CAAGTGGCAG TGTCCGGGTG
    CGGCCAGTGG TACCCGAGAG GAAGTCATCA CTCCCCCCGA CATCGCCTAT GGAGAAAATC
    TCCAAGTCCC GGTTGTCCTT TGACCTGCCA CTGACCTCCT CAACCAACCT GGATCTGTCT
    GGGATGAGCA TCTCCATCCG AAGCAAAACC AAGGTGAGCC GGCATCATTC AGATACAAAT
    TTTGGGGCTA AGCTGGCCCT GAAAAAGAGC CCCAACCAGC CATTTATGCC CATGGTCACT
    CAGTCTGACC TACGTTCCAT TCACCTGAGG TCTGTCAGCA AGTCTGAGCC GGAAGATGAC
    ATCGAGAGCC CTGACTATGC TGAGGACGCA GGAGCAGAGG TCTTCGTATT GCTGGAGAGA
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CTTGTGGCCC GAAGTCCTCC CAGCTTGGTC
    CACAAGCCAC CATCTGCCCC TGAGGAGTAC CCACTCACTT CACCGACCTT GGCTGTGACC
    CCCAAGATCT CTGTTCAACA CATGCGGCCA CTCCCCAAAG ACATCTACAC GGTGATGCGG
    AAACCAAAGT CCCCCGGCTT CCCCGAGGGC AGAGGCTCCA GGGAGCCACC AGCGCCCTCT
    TCTCTTGCTT TCACACCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GCACACTGGA GGATGGGGGC CCCAAGGTGA GAGCCCTGCC TGAAAGAATC
    AGCCTCCAGA GCCAGGAAGA AGCAGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTCCCC
    AAAAAACCCA GCGTGCTGTA CCTGCCTCTC ACATCACCCA CAGCACACAT GGAGGTGTTC
    AGGGCCGAAC TGAGGCTGCC CCACAGCCCC ATCATCACCC TGCAGGAAGA CACCAAGTGT
    CCGCCCACCA GTGACCCCCT GAAGGCACCT GGTGTGAGGC CAACTTCACC GCTGCAGGCT
    GAAGGTGAAA GGGAGGCAAG TCCTCTAGGG AGTTCTGTGG AACCGAGCAC TGCAGAAAAA
    AGCATAATCA GTGACAAAAC AGCTGAATGG ATCGCCGAAG ATGATGATGA CGTGTTTGTG
    GCTTCACGCA CAACCGAAGA TTTATTTACT GTGATACACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAAG AGCCTGGGGA GGCCTTTGCT GGTGGCAGCA GATCAAGCTC CCATTCACCA
    ATAAAGAACA CAGCTGATTT TTCCATCAGT GAGTCAGCTG TCTCTGCGGG GTCGAGTGGC
    AGCGCCAACC TGGATGCTGG CAGAAACAAT GATTTCAAGG CCTTGCTGCA AAAGAAGGGA
    AGCAAGGCAA CCCCCAAGTC CCGCCCCTCA GCTGCTGAAC TGCTCAAGAC CACTAATCCG
    CTGGCTCGGA GAATTATTGC ACAATTTTCC AAAGAGTATG AGACCACCAA CAACCCTGGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45526
    Clone ID Related Accession (Same CDS sequence) XM_014111509.1
    Accession Version XM_014111509.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGCAA CCCGCCGCAG 
    CCGCTCACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCCTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GCTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAA CCTGTTCCAG CGCACTGTGC GCCTCCGCCG CCGCCTTCCC
    TGCCACCTCC TCGGCCTGGA GGACGATGAG GAAGAGCTAG GTGCCTCCCA TTGGTTAAAC
    CTAACCCGAA ACCAGAGGGC AAGGGAACCT GTTGATGCAG TCCATACAGG TCATAGCAAC
    AGGCCTGCAG GCAGTGTGGC CCACTCTGCC ACCTCAGACA TCAAGTCCAG TCATTCCATC
    CCAGAAGGGG TTCATGGAAG AATTGCCATT GGTCAGGAAG CTCAGTTCCC AAATCTCACC
    TCACCAGTAC TGAGGAGTCC TTCTAGTGAT TCAGAAGAAC CTCTCCAGGC ATGTGGTGGC
    ACAAACCCTC CTGGTATGGA GAGCATAGGA ATGGTGTATA GCATCCCCAG TTCTTGCAAT
    GGACCAACGG AATCAACGTT CTCTGCTTCC TGGAAGGGAG ATACTTTTAC CTACATGACT
    CCAAGTGTCA CCAGTCAGAA CAATCAAATC AGTCAAAATG GAAGAAATCC TTCCTCTGGG
    AATGCTTGGG TCTCTCTGCA CACACTGCCA CCTCTAGTCC CCAAGGAGGC CACTACCTTC
    TTTGTTACCC GAGAGAACCC AACAGGATGC AGTGGGGCAA CTGGCAACTC TGAGCATCCT
    ACTCAACGAA GGCAAGTATC AGAGCGACCC TCCAAGATGG GCCTTCTCAC CAGTGGTACC
    TCAAGGCTGG AGACAGGCCG GGGGGGTGCC AGCAGGTTCC GGGAGCGGTC ACTGTCTGTG
    CCCATGGACT CTGGCACCAC GGATGTGGAC TATGATGAGG AACAGAAGGC CAGTGAAGCC
    TGTGCCCTGC CTTATGCCAG TACAAGCTCT GAGGGCAGTA ACAGTACTGA CAACATTGCC
    TCCCTTAGCA CCCAACAAGA GGCCCAGCCC AGAAGGCAGA GATCCAAGAG TATCTCACTC
    CGGAAGGCCA AGAAGAAGCC TTCCCCACCG ATGCGCAGTG TCTCACTGCT CAAAGATGGG
    CCAGTCCTCT TGCCAGAACA TGGGTCAGCG CTGCCCAAGG ACCAGAGGCC CAGGAGTCTC
    TGCCTCTCCT TGGAACACCA AGGGCGTCAG TCATCCCACC CAGATGCTCA GGGTCACCCA
    GCTGTGCCAA CCGTCAAAGA TCCAGAAGGT GCACAATTCT CCCACCACTG GTATCCTACT
    AACTGGAAGT CTGGCGACAC CTACCAATCC TTGTCCAGTT CCAGCACGGC CACTGGCACA
    ACAGTCATCG AGTGCACTCA AGTTCAGGGA AGCTCAGAGT CTCTTGCCTC CCCTTCCACA
    TCCAGAGCCA CGACACCTTC CCAACTCTCT ATCGAGGTGG AGGCCAGGGA AGTGTCCTCC
    CCAGGAAGGC CCACTGGGCT GATGTCACCT TCCAGTGGCT ACTCCAGCCA ATCAGAGACA
    CCAACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACC TGCCCCCTCC AAGTGGCAGT
    GTCCGGGTGC GGCCAGTGGT ACCCGAGAGG AAGTCATCAC TCCCCCCGAC ATCGCCTATG
    GAGAAAATCT CCAAGTCCCG GTTGTCCTTT GACCTGCCAC TGACCTCCTC AACCAACCTG
    GATCTGTCTG GGATGAGCAT CTCCATCCGA AGCAAAACCA AGGTGAGCCG GCATCATTCA
    GATACAAATT TTGGGGCTAA GCTGGCCCTG AAAAAGAGCC CCAACCAGCC ATTTATGCCC
    ATGGTCACTC AGTCTGACCT ACGTTCCATT CACCTGAGGT CTGTCAGCAA GTCTGAGCCG
    GAAGATGACA TCGAGAGCCC TGACTATGCT GAGGACGCAG GAGCAGAGGT CTTCGTATTG
    CTGGAGAGAA AGATGAAACC TCCCATAGCC GAGAAGCCCC TTGTGGCCCG AAGTCCTCCC
    AGCTTGGTCC ACAAGCCACC ATCTGCCCCT GAGGAGTACC CACTCACTTC ACCGACCTTG
    GCTGTGACCC CCAAGATCTC TGTTCAACAC ATGCGGCCAC TCCCCAAAGA CATCTACACG
    GTGATGCGGA AACCAAAGTC CCCCGGCTTC CCCGAGGGCA GAGGCTCCAG GGAGCCACCA
    GCGCCCTCTT CTCTTGCTTT CACACCTTTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGG CACACTGGAG GATGGGGGCC CCAAGGTGAG AGCCCTGCCT
    GAAAGAATCA GCCTCCAGAG CCAGGAAGAA GCAGAGAAAA AGAAAGGCAA GATTCCACCT
    CCTGTCCCCA AAAAACCCAG CGTGCTGTAC CTGCCTCTCA CATCACCCAC AGCACACATG
    GAGGTGTTCA GGGCCGAACT GAGGCTGCCC CACAGCCCCA TCATCACCCT GCAGGAAGAC
    ACCAAGTGTC CGCCCACCAG TGACCCCCTG AAGGCACCTG GTGTGAGGCC AACTTCACCG
    CTGCAGGCTG AAGGTGAAAG GGAGGCAAGT CCTCTAGGGA GTTCTGTGGA ACCGAGCACT
    GCAGAAAAAA GCATAATCAG TGACAAAACA GCTGAATGGA TCGCCGAAGA TGATGATGAC
    GTGTTTGTGG CTTCACGCAC AACCGAAGAT TTATTTACTG TGATACACAG GTCCAAAAGA
    AAGCTGCTTG GCTGGAAAGA GCCTGGGGAG GCCTTTGCTG GTGGCAGCAG ATCAAGCTCC
    CATTCACCAA TAAAGAACAC AGCTGATTTT TCCATCAGTG AGTCAGCTGT CTCTGCGGGG
    TCGAGTGGCA GCGCCAACCT GGATGCTGGC AGAAACAATG ATTTCAAGGC CTTGCTGCAA
    AAGAAGGGAA GCAAGGCAAC CCCCAAGTCC CGCCCCTCAG CTGCTGAACT GCTCAAGACC
    ACTAATCCGC TGGCTCGGAG AATTATTGCA CAATTTTCCA AAGAGTATGA GACCACCAAC
    AACCCTGGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966984.1
    CDS413..3547
    Translation

    Target ORF information:

    RefSeq Version XM_014111509.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111509.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGCAA CCCGCCGCAG 
    CCGCTCACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCCTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GCTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAA CCTGTTCCAG CGCACTGTGC GCCTCCGCCG CCGCCTTCCC
    TGCCACCTCC TCGGCCTGGA GGACGATGAG GAAGAGCTAG GTGCCTCCCA TTGGTTAAAC
    CTAACCCGAA ACCAGAGGGC AAGGGAACCT GTTGATGCAG TCCATACAGG TCATAGCAAC
    AGGCCTGCAG GCAGTGTGGC CCACTCTGCC ACCTCAGACA TCAAGTCCAG TCATTCCATC
    CCAGAAGGGG TTCATGGAAG AATTGCCATT GGTCAGGAAG CTCAGTTCCC AAATCTCACC
    TCACCAGTAC TGAGGAGTCC TTCTAGTGAT TCAGAAGAAC CTCTCCAGGC ATGTGGTGGC
    ACAAACCCTC CTGGTATGGA GAGCATAGGA ATGGTGTATA GCATCCCCAG TTCTTGCAAT
    GGACCAACGG AATCAACGTT CTCTGCTTCC TGGAAGGGAG ATACTTTTAC CTACATGACT
    CCAAGTGTCA CCAGTCAGAA CAATCAAATC AGTCAAAATG GAAGAAATCC TTCCTCTGGG
    AATGCTTGGG TCTCTCTGCA CACACTGCCA CCTCTAGTCC CCAAGGAGGC CACTACCTTC
    TTTGTTACCC GAGAGAACCC AACAGGATGC AGTGGGGCAA CTGGCAACTC TGAGCATCCT
    ACTCAACGAA GGCAAGTATC AGAGCGACCC TCCAAGATGG GCCTTCTCAC CAGTGGTACC
    TCAAGGCTGG AGACAGGCCG GGGGGGTGCC AGCAGGTTCC GGGAGCGGTC ACTGTCTGTG
    CCCATGGACT CTGGCACCAC GGATGTGGAC TATGATGAGG AACAGAAGGC CAGTGAAGCC
    TGTGCCCTGC CTTATGCCAG TACAAGCTCT GAGGGCAGTA ACAGTACTGA CAACATTGCC
    TCCCTTAGCA CCCAACAAGA GGCCCAGCCC AGAAGGCAGA GATCCAAGAG TATCTCACTC
    CGGAAGGCCA AGAAGAAGCC TTCCCCACCG ATGCGCAGTG TCTCACTGCT CAAAGATGGG
    CCAGTCCTCT TGCCAGAACA TGGGTCAGCG CTGCCCAAGG ACCAGAGGCC CAGGAGTCTC
    TGCCTCTCCT TGGAACACCA AGGGCGTCAG TCATCCCACC CAGATGCTCA GGGTCACCCA
    GCTGTGCCAA CCGTCAAAGA TCCAGAAGGT GCACAATTCT CCCACCACTG GTATCCTACT
    AACTGGAAGT CTGGCGACAC CTACCAATCC TTGTCCAGTT CCAGCACGGC CACTGGCACA
    ACAGTCATCG AGTGCACTCA AGTTCAGGGA AGCTCAGAGT CTCTTGCCTC CCCTTCCACA
    TCCAGAGCCA CGACACCTTC CCAACTCTCT ATCGAGGTGG AGGCCAGGGA AGTGTCCTCC
    CCAGGAAGGC CCACTGGGCT GATGTCACCT TCCAGTGGCT ACTCCAGCCA ATCAGAGACA
    CCAACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACC TGCCCCCTCC AAGTGGCAGT
    GTCCGGGTGC GGCCAGTGGT ACCCGAGAGG AAGTCATCAC TCCCCCCGAC ATCGCCTATG
    GAGAAAATCT CCAAGTCCCG GTTGTCCTTT GACCTGCCAC TGACCTCCTC AACCAACCTG
    GATCTGTCTG GGATGAGCAT CTCCATCCGA AGCAAAACCA AGGTGAGCCG GCATCATTCA
    GATACAAATT TTGGGGCTAA GCTGGCCCTG AAAAAGAGCC CCAACCAGCC ATTTATGCCC
    ATGGTCACTC AGTCTGACCT ACGTTCCATT CACCTGAGGT CTGTCAGCAA GTCTGAGCCG
    GAAGATGACA TCGAGAGCCC TGACTATGCT GAGGACGCAG GAGCAGAGGT CTTCGTATTG
    CTGGAGAGAA AGATGAAACC TCCCATAGCC GAGAAGCCCC TTGTGGCCCG AAGTCCTCCC
    AGCTTGGTCC ACAAGCCACC ATCTGCCCCT GAGGAGTACC CACTCACTTC ACCGACCTTG
    GCTGTGACCC CCAAGATCTC TGTTCAACAC ATGCGGCCAC TCCCCAAAGA CATCTACACG
    GTGATGCGGA AACCAAAGTC CCCCGGCTTC CCCGAGGGCA GAGGCTCCAG GGAGCCACCA
    GCGCCCTCTT CTCTTGCTTT CACACCTTTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGG CACACTGGAG GATGGGGGCC CCAAGGTGAG AGCCCTGCCT
    GAAAGAATCA GCCTCCAGAG CCAGGAAGAA GCAGAGAAAA AGAAAGGCAA GATTCCACCT
    CCTGTCCCCA AAAAACCCAG CGTGCTGTAC CTGCCTCTCA CATCACCCAC AGCACACATG
    GAGGTGTTCA GGGCCGAACT GAGGCTGCCC CACAGCCCCA TCATCACCCT GCAGGAAGAC
    ACCAAGTGTC CGCCCACCAG TGACCCCCTG AAGGCACCTG GTGTGAGGCC AACTTCACCG
    CTGCAGGCTG AAGGTGAAAG GGAGGCAAGT CCTCTAGGGA GTTCTGTGGA ACCGAGCACT
    GCAGAAAAAA GCATAATCAG TGACAAAACA GCTGAATGGA TCGCCGAAGA TGATGATGAC
    GTGTTTGTGG CTTCACGCAC AACCGAAGAT TTATTTACTG TGATACACAG GTCCAAAAGA
    AAGCTGCTTG GCTGGAAAGA GCCTGGGGAG GCCTTTGCTG GTGGCAGCAG ATCAAGCTCC
    CATTCACCAA TAAAGAACAC AGCTGATTTT TCCATCAGTG AGTCAGCTGT CTCTGCGGGG
    TCGAGTGGCA GCGCCAACCT GGATGCTGGC AGAAACAATG ATTTCAAGGC CTTGCTGCAA
    AAGAAGGGAA GCAAGGCAAC CCCCAAGTCC CGCCCCTCAG CTGCTGAACT GCTCAAGACC
    ACTAATCCGC TGGCTCGGAG AATTATTGCA CAATTTTCCA AAGAGTATGA GACCACCAAC
    AACCCTGGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32539
    Clone ID Related Accession (Same CDS sequence) XM_549074.5
    Accession Version XM_549074.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_549074.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGCAA CCCGCCGCAG 
    CCGCTCACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCCTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GCTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAA CCTGTTCCAG CGCACTGTGC GCCTCCGCCG CCGCCTTCCC
    TGCCACCTCC TCGGCCTGGA GGACGATGAG GAAGAGCTAG CTGCAGCTAA CTCGGGTCGG
    GAAAATGCGA CAGCGACTGC CCACTCGAGG TCGTCATGGC GACAGCCAGT GAACGTGTTC
    CTCTCCTCGG GCAGGCCCCC GAGTGTAGAG GAGCTGCTTC GAGAGGCGCA GCTCAATCTC
    CAGAGCCTGT TGCAAGAAGA ATATGAGGAA CAGTACTCAG AGGCCAGACT TCTGGGGCAG
    ACCTTCCGCT CTGCTGATGA GGCCCCCGAG GCCACTCCTA GCCCAAGGCC CCAGGCTGGC
    AGGCGTCTGG AGCTTGTATT GATGCCTACA AAACGGCAGC TGAGCGAGGA TGAGACTACC
    ACCCAGGGTG TGAGGGCCCC TGAGGCCTCC TCGAGCCTGT CTACCACAGC CGACAAGCAA
    GCTGCCTGGA ATAGCCCCTT CCCTCTGCCC ATCCTAGAGG AGAAGCGGTG GCTTCAGCCT
    TGCTCCACGC AATCTGACAT TGTGCCCATC AACATCTCTG GGCAGCAGTT TGATAAACAT
    GCAAGTTTGC GACACTCGTT GTTTAACACA GAGACAGCTG TGAACCCCAA ATCCACCCTG
    AGGCGGAGGC GGACCATTAT TGGATTCTCT AACTTTTCCC AGCGAGACCA AGGTCATAGC
    AACAGGCCTG CAGGCAGTGT GGCCCACTCT GCCACCTCAG ACATCAAGTC CAGTCATTCC
    ATCCCAGAAG GGGTTCATGG AAGAATTGCC ATTGGTCAGG AAGCTCAGTT CCCAAATCTC
    ACCTCACCAG TACTGAGGAG TCCTTCTAGT GATTCAGAAG AACCTCTCCA GGCATGTGGT
    GGCACAAACC CTCCTGGTAT GGAGAGCATA GGAATGGTGT ATAGCATCCC CAGTTCTTGC
    AATGGACCAA CGGAATCAAC GTTCTCTGCT TCCTGGAAGG GAGATACTTT TACCTACATG
    ACTCCAAGTG TCACCAGTCA GAACAATCAA ATCAGTCAAA ATGGAAGAAA TCCTTCCTCT
    GGGAATGCTT GGGTCTCTCT GCACACACTG CCACCTCTAG TCCCCAAGGA GGCCACTACC
    TTCTTTGTTA CCCGAGAGAA CCCAACAGGA TGCAGTGGGG CAACTGGCAA CTCTGAGCAT
    CCTACTCAAC GAAGGCAAGT ATCAGAGCGA CCCTCCAAGA TGGGCCTTCT CACCAGTGGT
    ACCTCAAGGC TGGAGACAGG CCGGGGGGGT GCCAGCAGGT TCCGGGAGCG GTCACTGTCT
    GTGCCCATGG ACTCTGGCAC CACGGATGTG GACTATGATG AGGAACAGAA GGCCAGTGAA
    GCCTGTGCCC TGCCTTATGC CAGTACAAGC TCTGAGGGCA GTAACAGTAC TGACAACATT
    GCCTCCCTTA GCACCCAACA AGAGGCCCAG CCCAGAAGGC AGAGATCCAA GAGTATCTCA
    CTCCGGAAGG CCAAGAAGAA GCCTTCCCCA CCGATGCGCA GTGTCTCACT GCTCAAAGAT
    GGGCCAGTCC TCTTGCCAGA ACATGGGTCA GCGCTGCCCA AGGACCAGAG GCCCAGGAGT
    CTCTGCCTCT CCTTGGAACA CCAAGGGCGT CAGTCATCCC ACCCAGATGC TCAGGGTCAC
    CCAGCTGTGC CAACCGTCAA AGATCCAGAA GGTGCACAAT TCTCCCACCA CTGGTATCCT
    ACTAACTGGA AGTCTGGCGA CACCTACCAA TCCTTGTCCA GTTCCAGCAC GGCCACTGGC
    ACAACAGTCA TCGAGTGCAC TCAAGTTCAG GGAAGCTCAG AGTCTCTTGC CTCCCCTTCC
    ACATCCAGAG CCACGACACC TTCCCAACTC TCTATCGAGG TGGAGGCCAG GGAAGTGTCC
    TCCCCAGGAA GGCCCACTGG GCTGATGTCA CCTTCCAGTG GCTACTCCAG CCAATCAGAG
    ACACCAACAC CCACTGTCTC CATGTCCTTG ACCCTGGGCC ACCTGCCCCC TCCAAGTGGC
    AGTGTCCGGG TGCGGCCAGT GGTACCCGAG AGGAAGTCAT CACTCCCCCC GACATCGCCT
    ATGGAGAAAA TCTCCAAGTC CCGGTTGTCC TTTGACCTGC CACTGACCTC CTCAACCAAC
    CTGGATCTGT CTGGGATGAG CATCTCCATC CGAAGCAAAA CCAAGGTGAG CCGGCATCAT
    TCAGATACAA ATTTTGGGGC TAAGCTGGCC CTGAAAAAGA GCCCCAACCA GCCATTTATG
    CCCATGGTCA CTCAGTCTGA CCTACGTTCC ATTCACCTGA GGTCTGTCAG CAAGTCTGAG
    CCGGAAGATG ACATCGAGAG CCCTGACTAT GCTGAGGACG CAGGAGCAGA GGTCTTCGTA
    TTGCTGGAGA GAAAGATGAA ACCTCCCATA GCCGAGAAGC CCCTTGTGGC CCGAAGTCCT
    CCCAGCTTGG TCCACAAGCC ACCATCTGCC CCTGAGGAGT ACCCACTCAC TTCACCGACC
    TTGGCTGTGA CCCCCAAGAT CTCTGTTCAA CACATGCGGC CACTCCCCAA AGACATCTAC
    ACGGTGATGC GGAAACCAAA GTCCCCCGGC TTCCCCGAGG GCAGAGGCTC CAGGGAGCCA
    CCAGCGCCCT CTTCTCTTGC TTTCACACCT TTTGCCAGTT CCTCTGGTGC TTTCTTCTCA
    GGAACACAGC AACCTCCCCA GGGCACACTG GAGGATGGGG GCCCCAAGGT GAGAGCCCTG
    CCTGAAAGAA TCAGCCTCCA GAGCCAGGAA GAAGCAGAGA AAAAGAAAGG CAAGATTCCA
    CCTCCTGTCC CCAAAAAACC CAGCGTGCTG TACCTGCCTC TCACATCACC CACAGCACAC
    ATGGAGGTGT TCAGGGCCGA ACTGAGGCTG CCCCACAGCC CCATCATCAC CCTGCAGGAA
    GACACCAAGT GTCCGCCCAC CAGTGACCCC CTGAAGGCAC CTGGTGTGAG GCCAACTTCA
    CCGCTGCAGG CTGAAGGTGA AAGGGAGGCA AGTCCTCTAG GGAGTTCTGT GGAACCGAGC
    ACTGCAGAAA AAAGCATAAT CAGTGACAAA ACAGCTGAAT GGATCGCCGA AGATGATGAT
    GACGTGTTTG TGGCTTCACG CACAACCGAA GATTTATTTA CTGTGATACA CAGGTCCAAA
    AGAAAGCTGC TTGGCTGGAA AGAGCCTGGG GAGGCCTTTG CTGGTGGCAG CAGATCAAGC
    TCCCATTCAC CAATAAAGAA CACAGCTGAT TTTTCCATCA GTGAGTCAGC TGTCTCTGCG
    GGGTCGAGTG GCAGCGCCAA CCTGGATGCT GGCAGAAACA ATGATTTCAA GGCCTTGCTG
    CAAAAGAAGG GAAGCAAGGC AACCCCCAAG TCCCGCCCCT CAGCTGCTGA ACTGCTCAAG
    ACCACTAATC CGCTGGCTCG GAGAATTATT GCACAATTTT CCAAAGAGTA TGAGACCACC
    AACAACCCTG GTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_549074.3
    CDS416..4093
    Translation

    Target ORF information:

    RefSeq Version XM_549074.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_549074.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGCAA CCCGCCGCAG 
    CCGCTCACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCCTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GCTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAA CCTGTTCCAG CGCACTGTGC GCCTCCGCCG CCGCCTTCCC
    TGCCACCTCC TCGGCCTGGA GGACGATGAG GAAGAGCTAG CTGCAGCTAA CTCGGGTCGG
    GAAAATGCGA CAGCGACTGC CCACTCGAGG TCGTCATGGC GACAGCCAGT GAACGTGTTC
    CTCTCCTCGG GCAGGCCCCC GAGTGTAGAG GAGCTGCTTC GAGAGGCGCA GCTCAATCTC
    CAGAGCCTGT TGCAAGAAGA ATATGAGGAA CAGTACTCAG AGGCCAGACT TCTGGGGCAG
    ACCTTCCGCT CTGCTGATGA GGCCCCCGAG GCCACTCCTA GCCCAAGGCC CCAGGCTGGC
    AGGCGTCTGG AGCTTGTATT GATGCCTACA AAACGGCAGC TGAGCGAGGA TGAGACTACC
    ACCCAGGGTG TGAGGGCCCC TGAGGCCTCC TCGAGCCTGT CTACCACAGC CGACAAGCAA
    GCTGCCTGGA ATAGCCCCTT CCCTCTGCCC ATCCTAGAGG AGAAGCGGTG GCTTCAGCCT
    TGCTCCACGC AATCTGACAT TGTGCCCATC AACATCTCTG GGCAGCAGTT TGATAAACAT
    GCAAGTTTGC GACACTCGTT GTTTAACACA GAGACAGCTG TGAACCCCAA ATCCACCCTG
    AGGCGGAGGC GGACCATTAT TGGATTCTCT AACTTTTCCC AGCGAGACCA AGGTCATAGC
    AACAGGCCTG CAGGCAGTGT GGCCCACTCT GCCACCTCAG ACATCAAGTC CAGTCATTCC
    ATCCCAGAAG GGGTTCATGG AAGAATTGCC ATTGGTCAGG AAGCTCAGTT CCCAAATCTC
    ACCTCACCAG TACTGAGGAG TCCTTCTAGT GATTCAGAAG AACCTCTCCA GGCATGTGGT
    GGCACAAACC CTCCTGGTAT GGAGAGCATA GGAATGGTGT ATAGCATCCC CAGTTCTTGC
    AATGGACCAA CGGAATCAAC GTTCTCTGCT TCCTGGAAGG GAGATACTTT TACCTACATG
    ACTCCAAGTG TCACCAGTCA GAACAATCAA ATCAGTCAAA ATGGAAGAAA TCCTTCCTCT
    GGGAATGCTT GGGTCTCTCT GCACACACTG CCACCTCTAG TCCCCAAGGA GGCCACTACC
    TTCTTTGTTA CCCGAGAGAA CCCAACAGGA TGCAGTGGGG CAACTGGCAA CTCTGAGCAT
    CCTACTCAAC GAAGGCAAGT ATCAGAGCGA CCCTCCAAGA TGGGCCTTCT CACCAGTGGT
    ACCTCAAGGC TGGAGACAGG CCGGGGGGGT GCCAGCAGGT TCCGGGAGCG GTCACTGTCT
    GTGCCCATGG ACTCTGGCAC CACGGATGTG GACTATGATG AGGAACAGAA GGCCAGTGAA
    GCCTGTGCCC TGCCTTATGC CAGTACAAGC TCTGAGGGCA GTAACAGTAC TGACAACATT
    GCCTCCCTTA GCACCCAACA AGAGGCCCAG CCCAGAAGGC AGAGATCCAA GAGTATCTCA
    CTCCGGAAGG CCAAGAAGAA GCCTTCCCCA CCGATGCGCA GTGTCTCACT GCTCAAAGAT
    GGGCCAGTCC TCTTGCCAGA ACATGGGTCA GCGCTGCCCA AGGACCAGAG GCCCAGGAGT
    CTCTGCCTCT CCTTGGAACA CCAAGGGCGT CAGTCATCCC ACCCAGATGC TCAGGGTCAC
    CCAGCTGTGC CAACCGTCAA AGATCCAGAA GGTGCACAAT TCTCCCACCA CTGGTATCCT
    ACTAACTGGA AGTCTGGCGA CACCTACCAA TCCTTGTCCA GTTCCAGCAC GGCCACTGGC
    ACAACAGTCA TCGAGTGCAC TCAAGTTCAG GGAAGCTCAG AGTCTCTTGC CTCCCCTTCC
    ACATCCAGAG CCACGACACC TTCCCAACTC TCTATCGAGG TGGAGGCCAG GGAAGTGTCC
    TCCCCAGGAA GGCCCACTGG GCTGATGTCA CCTTCCAGTG GCTACTCCAG CCAATCAGAG
    ACACCAACAC CCACTGTCTC CATGTCCTTG ACCCTGGGCC ACCTGCCCCC TCCAAGTGGC
    AGTGTCCGGG TGCGGCCAGT GGTACCCGAG AGGAAGTCAT CACTCCCCCC GACATCGCCT
    ATGGAGAAAA TCTCCAAGTC CCGGTTGTCC TTTGACCTGC CACTGACCTC CTCAACCAAC
    CTGGATCTGT CTGGGATGAG CATCTCCATC CGAAGCAAAA CCAAGGTGAG CCGGCATCAT
    TCAGATACAA ATTTTGGGGC TAAGCTGGCC CTGAAAAAGA GCCCCAACCA GCCATTTATG
    CCCATGGTCA CTCAGTCTGA CCTACGTTCC ATTCACCTGA GGTCTGTCAG CAAGTCTGAG
    CCGGAAGATG ACATCGAGAG CCCTGACTAT GCTGAGGACG CAGGAGCAGA GGTCTTCGTA
    TTGCTGGAGA GAAAGATGAA ACCTCCCATA GCCGAGAAGC CCCTTGTGGC CCGAAGTCCT
    CCCAGCTTGG TCCACAAGCC ACCATCTGCC CCTGAGGAGT ACCCACTCAC TTCACCGACC
    TTGGCTGTGA CCCCCAAGAT CTCTGTTCAA CACATGCGGC CACTCCCCAA AGACATCTAC
    ACGGTGATGC GGAAACCAAA GTCCCCCGGC TTCCCCGAGG GCAGAGGCTC CAGGGAGCCA
    CCAGCGCCCT CTTCTCTTGC TTTCACACCT TTTGCCAGTT CCTCTGGTGC TTTCTTCTCA
    GGAACACAGC AACCTCCCCA GGGCACACTG GAGGATGGGG GCCCCAAGGT GAGAGCCCTG
    CCTGAAAGAA TCAGCCTCCA GAGCCAGGAA GAAGCAGAGA AAAAGAAAGG CAAGATTCCA
    CCTCCTGTCC CCAAAAAACC CAGCGTGCTG TACCTGCCTC TCACATCACC CACAGCACAC
    ATGGAGGTGT TCAGGGCCGA ACTGAGGCTG CCCCACAGCC CCATCATCAC CCTGCAGGAA
    GACACCAAGT GTCCGCCCAC CAGTGACCCC CTGAAGGCAC CTGGTGTGAG GCCAACTTCA
    CCGCTGCAGG CTGAAGGTGA AAGGGAGGCA AGTCCTCTAG GGAGTTCTGT GGAACCGAGC
    ACTGCAGAAA AAAGCATAAT CAGTGACAAA ACAGCTGAAT GGATCGCCGA AGATGATGAT
    GACGTGTTTG TGGCTTCACG CACAACCGAA GATTTATTTA CTGTGATACA CAGGTCCAAA
    AGAAAGCTGC TTGGCTGGAA AGAGCCTGGG GAGGCCTTTG CTGGTGGCAG CAGATCAAGC
    TCCCATTCAC CAATAAAGAA CACAGCTGAT TTTTCCATCA GTGAGTCAGC TGTCTCTGCG
    GGGTCGAGTG GCAGCGCCAA CCTGGATGCT GGCAGAAACA ATGATTTCAA GGCCTTGCTG
    CAAAAGAAGG GAAGCAAGGC AACCCCCAAG TCCCGCCCCT CAGCTGCTGA ACTGCTCAAG
    ACCACTAATC CGCTGGCTCG GAGAATTATT GCACAATTTT CCAAAGAGTA TGAGACCACC
    AACAACCCTG GTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32540
    Clone ID Related Accession (Same CDS sequence) XM_005641480.3 , XM_005641480.2
    Accession Version XM_005641480.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NHS-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641480.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGAGAG CCGAGTTTGT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGAGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTACT CAGAGGCCAG ACTTCTGGGG
    CAGACCTTCC GCTCTGCTGA TGAGGCCCCC GAGGCCACTC CTAGCCCAAG GCCCCAGGCT
    GGCAGGCGTC TGGAGCTTGT ATTGATGCCT ACAAAACGGC AGCTGAGCGA GGATGAGACT
    ACCACCCAGG GTGTGAGGGC CCCTGAGGCC TCCTCGAGCC TGTCTACCAC AGCCGACAAG
    CAAGCTGCCT GGAATAGCCC CTTCCCTCTG CCCATCCTAG AGGAGAAGCG GTGGCTTCAG
    CCTTGCTCCA CGCAATCTGA CATTGTGCCC ATCAACATCT CTGGGCAGCA GTTTGATAAA
    CATGCAAGTT TGCGACACTC GTTGTTTAAC ACAGAGACAG CTGTGAACCC CAAATCCACC
    CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTTTT CCCAGCGAGA CCAAGGTCAT
    AGCAACAGGC CTGCAGGCAG TGTGGCCCAC TCTGCCACCT CAGACATCAA GTCCAGTCAT
    TCCATCCCAG AAGGGGTTCA TGGAAGAATT GCCATTGGTC AGGAAGCTCA GTTCCCAAAT
    CTCACCTCAC CAGTACTGAG GAGTCCTTCT AGTGATTCAG AAGAACCTCT CCAGGCATGT
    GGTGGCACAA ACCCTCCTGG TATGGAGAGC ATAGGAATGG TGTATAGCAT CCCCAGTTCT
    TGCAATGGAC CAACGGAATC AACGTTCTCT GCTTCCTGGA AGGGAGATAC TTTTACCTAC
    ATGACTCCAA GTGTCACCAG TCAGAACAAT CAAATCAGTC AAAATGGAAG AAATCCTTCC
    TCTGGGAATG CTTGGGTCTC TCTGCACACA CTGCCACCTC TAGTCCCCAA GGAGGCCACT
    ACCTTCTTTG TTACCCGAGA GAACCCAACA GGATGCAGTG GGGCAACTGG CAACTCTGAG
    CATCCTACTC AACGAAGGCA AGTATCAGAG CGACCCTCCA AGATGGGCCT TCTCACCAGT
    GGTACCTCAA GGCTGGAGAC AGGCCGGGGG GGTGCCAGCA GGTTCCGGGA GCGGTCACTG
    TCTGTGCCCA TGGACTCTGG CACCACGGAT GTGGACTATG ATGAGGAACA GAAGGCCAGT
    GAAGCCTGTG CCCTGCCTTA TGCCAGTACA AGCTCTGAGG GCAGTAACAG TACTGACAAC
    ATTGCCTCCC TTAGCACCCA ACAAGAGGCC CAGCCCAGAA GGCAGAGATC CAAGAGTATC
    TCACTCCGGA AGGCCAAGAA GAAGCCTTCC CCACCGATGC GCAGTGTCTC ACTGCTCAAA
    GATGGGCCAG TCCTCTTGCC AGAACATGGG TCAGCGCTGC CCAAGGACCA GAGGCCCAGG
    AGTCTCTGCC TCTCCTTGGA ACACCAAGGG CGTCAGTCAT CCCACCCAGA TGCTCAGGGT
    CACCCAGCTG TGCCAACCGT CAAAGATCCA GAAGGTGCAC AATTCTCCCA CCACTGGTAT
    CCTACTAACT GGAAGTCTGG CGACACCTAC CAATCCTTGT CCAGTTCCAG CACGGCCACT
    GGCACAACAG TCATCGAGTG CACTCAAGTT CAGGGAAGCT CAGAGTCTCT TGCCTCCCCT
    TCCACATCCA GAGCCACGAC ACCTTCCCAA CTCTCTATCG AGGTGGAGGC CAGGGAAGTG
    TCCTCCCCAG GAAGGCCCAC TGGGCTGATG TCACCTTCCA GTGGCTACTC CAGCCAATCA
    GAGACACCAA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACCTGCC CCCTCCAAGT
    GGCAGTGTCC GGGTGCGGCC AGTGGTACCC GAGAGGAAGT CATCACTCCC CCCGACATCG
    CCTATGGAGA AAATCTCCAA GTCCCGGTTG TCCTTTGACC TGCCACTGAC CTCCTCAACC
    AACCTGGATC TGTCTGGGAT GAGCATCTCC ATCCGAAGCA AAACCAAGGT GAGCCGGCAT
    CATTCAGATA CAAATTTTGG GGCTAAGCTG GCCCTGAAAA AGAGCCCCAA CCAGCCATTT
    ATGCCCATGG TCACTCAGTC TGACCTACGT TCCATTCACC TGAGGTCTGT CAGCAAGTCT
    GAGCCGGAAG ATGACATCGA GAGCCCTGAC TATGCTGAGG ACGCAGGAGC AGAGGTCTTC
    GTATTGCTGG AGAGAAAGAT GAAACCTCCC ATAGCCGAGA AGCCCCTTGT GGCCCGAAGT
    CCTCCCAGCT TGGTCCACAA GCCACCATCT GCCCCTGAGG AGTACCCACT CACTTCACCG
    ACCTTGGCTG TGACCCCCAA GATCTCTGTT CAACACATGC GGCCACTCCC CAAAGACATC
    TACACGGTGA TGCGGAAACC AAAGTCCCCC GGCTTCCCCG AGGGCAGAGG CTCCAGGGAG
    CCACCAGCGC CCTCTTCTCT TGCTTTCACA CCTTTTGCCA GTTCCTCTGG TGCTTTCTTC
    TCAGGAACAC AGCAACCTCC CCAGGGCACA CTGGAGGATG GGGGCCCCAA GGTGAGAGCC
    CTGCCTGAAA GAATCAGCCT CCAGAGCCAG GAAGAAGCAG AGAAAAAGAA AGGCAAGATT
    CCACCTCCTG TCCCCAAAAA ACCCAGCGTG CTGTACCTGC CTCTCACATC ACCCACAGCA
    CACATGGAGG TGTTCAGGGC CGAACTGAGG CTGCCCCACA GCCCCATCAT CACCCTGCAG
    GAAGACACCA AGTGTCCGCC CACCAGTGAC CCCCTGAAGG CACCTGGTGT GAGGCCAACT
    TCACCGCTGC AGGCTGAAGG TGAAAGGGAG GCAAGTCCTC TAGGGAGTTC TGTGGAACCG
    AGCACTGCAG AAAAAAGCAT AATCAGTGAC AAAACAGCTG AATGGATCGC CGAAGATGAT
    GATGACGTGT TTGTGGCTTC ACGCACAACC GAAGATTTAT TTACTGTGAT ACACAGGTCC
    AAAAGAAAGC TGCTTGGCTG GAAAGAGCCT GGGGAGGCCT TTGCTGGTGG CAGCAGATCA
    AGCTCCCATT CACCAATAAA GAACACAGCT GATTTTTCCA TCAGTGAGTC AGCTGTCTCT
    GCGGGGTCGA GTGGCAGCGC CAACCTGGAT GCTGGCAGAA ACAATGATTT CAAGGCCTTG
    CTGCAAAAGA AGGGAAGCAA GGCAACCCCC AAGTCCCGCC CCTCAGCTGC TGAACTGCTC
    AAGACCACTA ATCCGCTGGC TCGGAGAATT ATTGCACAAT TTTCCAAAGA GTATGAGACC
    ACCAACAACC CTGGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641537.1
    CDS499..3939
    Translation

    Target ORF information:

    RefSeq Version XM_005641480.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NHS like 2 (LOC491954), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641480.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGAGAG CCGAGTTTGT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGAGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTACT CAGAGGCCAG ACTTCTGGGG
    CAGACCTTCC GCTCTGCTGA TGAGGCCCCC GAGGCCACTC CTAGCCCAAG GCCCCAGGCT
    GGCAGGCGTC TGGAGCTTGT ATTGATGCCT ACAAAACGGC AGCTGAGCGA GGATGAGACT
    ACCACCCAGG GTGTGAGGGC CCCTGAGGCC TCCTCGAGCC TGTCTACCAC AGCCGACAAG
    CAAGCTGCCT GGAATAGCCC CTTCCCTCTG CCCATCCTAG AGGAGAAGCG GTGGCTTCAG
    CCTTGCTCCA CGCAATCTGA CATTGTGCCC ATCAACATCT CTGGGCAGCA GTTTGATAAA
    CATGCAAGTT TGCGACACTC GTTGTTTAAC ACAGAGACAG CTGTGAACCC CAAATCCACC
    CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTTTT CCCAGCGAGA CCAAGGTCAT
    AGCAACAGGC CTGCAGGCAG TGTGGCCCAC TCTGCCACCT CAGACATCAA GTCCAGTCAT
    TCCATCCCAG AAGGGGTTCA TGGAAGAATT GCCATTGGTC AGGAAGCTCA GTTCCCAAAT
    CTCACCTCAC CAGTACTGAG GAGTCCTTCT AGTGATTCAG AAGAACCTCT CCAGGCATGT
    GGTGGCACAA ACCCTCCTGG TATGGAGAGC ATAGGAATGG TGTATAGCAT CCCCAGTTCT
    TGCAATGGAC CAACGGAATC AACGTTCTCT GCTTCCTGGA AGGGAGATAC TTTTACCTAC
    ATGACTCCAA GTGTCACCAG TCAGAACAAT CAAATCAGTC AAAATGGAAG AAATCCTTCC
    TCTGGGAATG CTTGGGTCTC TCTGCACACA CTGCCACCTC TAGTCCCCAA GGAGGCCACT
    ACCTTCTTTG TTACCCGAGA GAACCCAACA GGATGCAGTG GGGCAACTGG CAACTCTGAG
    CATCCTACTC AACGAAGGCA AGTATCAGAG CGACCCTCCA AGATGGGCCT TCTCACCAGT
    GGTACCTCAA GGCTGGAGAC AGGCCGGGGG GGTGCCAGCA GGTTCCGGGA GCGGTCACTG
    TCTGTGCCCA TGGACTCTGG CACCACGGAT GTGGACTATG ATGAGGAACA GAAGGCCAGT
    GAAGCCTGTG CCCTGCCTTA TGCCAGTACA AGCTCTGAGG GCAGTAACAG TACTGACAAC
    ATTGCCTCCC TTAGCACCCA ACAAGAGGCC CAGCCCAGAA GGCAGAGATC CAAGAGTATC
    TCACTCCGGA AGGCCAAGAA GAAGCCTTCC CCACCGATGC GCAGTGTCTC ACTGCTCAAA
    GATGGGCCAG TCCTCTTGCC AGAACATGGG TCAGCGCTGC CCAAGGACCA GAGGCCCAGG
    AGTCTCTGCC TCTCCTTGGA ACACCAAGGG CGTCAGTCAT CCCACCCAGA TGCTCAGGGT
    CACCCAGCTG TGCCAACCGT CAAAGATCCA GAAGGTGCAC AATTCTCCCA CCACTGGTAT
    CCTACTAACT GGAAGTCTGG CGACACCTAC CAATCCTTGT CCAGTTCCAG CACGGCCACT
    GGCACAACAG TCATCGAGTG CACTCAAGTT CAGGGAAGCT CAGAGTCTCT TGCCTCCCCT
    TCCACATCCA GAGCCACGAC ACCTTCCCAA CTCTCTATCG AGGTGGAGGC CAGGGAAGTG
    TCCTCCCCAG GAAGGCCCAC TGGGCTGATG TCACCTTCCA GTGGCTACTC CAGCCAATCA
    GAGACACCAA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACCTGCC CCCTCCAAGT
    GGCAGTGTCC GGGTGCGGCC AGTGGTACCC GAGAGGAAGT CATCACTCCC CCCGACATCG
    CCTATGGAGA AAATCTCCAA GTCCCGGTTG TCCTTTGACC TGCCACTGAC CTCCTCAACC
    AACCTGGATC TGTCTGGGAT GAGCATCTCC ATCCGAAGCA AAACCAAGGT GAGCCGGCAT
    CATTCAGATA CAAATTTTGG GGCTAAGCTG GCCCTGAAAA AGAGCCCCAA CCAGCCATTT
    ATGCCCATGG TCACTCAGTC TGACCTACGT TCCATTCACC TGAGGTCTGT CAGCAAGTCT
    GAGCCGGAAG ATGACATCGA GAGCCCTGAC TATGCTGAGG ACGCAGGAGC AGAGGTCTTC
    GTATTGCTGG AGAGAAAGAT GAAACCTCCC ATAGCCGAGA AGCCCCTTGT GGCCCGAAGT
    CCTCCCAGCT TGGTCCACAA GCCACCATCT GCCCCTGAGG AGTACCCACT CACTTCACCG
    ACCTTGGCTG TGACCCCCAA GATCTCTGTT CAACACATGC GGCCACTCCC CAAAGACATC
    TACACGGTGA TGCGGAAACC AAAGTCCCCC GGCTTCCCCG AGGGCAGAGG CTCCAGGGAG
    CCACCAGCGC CCTCTTCTCT TGCTTTCACA CCTTTTGCCA GTTCCTCTGG TGCTTTCTTC
    TCAGGAACAC AGCAACCTCC CCAGGGCACA CTGGAGGATG GGGGCCCCAA GGTGAGAGCC
    CTGCCTGAAA GAATCAGCCT CCAGAGCCAG GAAGAAGCAG AGAAAAAGAA AGGCAAGATT
    CCACCTCCTG TCCCCAAAAA ACCCAGCGTG CTGTACCTGC CTCTCACATC ACCCACAGCA
    CACATGGAGG TGTTCAGGGC CGAACTGAGG CTGCCCCACA GCCCCATCAT CACCCTGCAG
    GAAGACACCA AGTGTCCGCC CACCAGTGAC CCCCTGAAGG CACCTGGTGT GAGGCCAACT
    TCACCGCTGC AGGCTGAAGG TGAAAGGGAG GCAAGTCCTC TAGGGAGTTC TGTGGAACCG
    AGCACTGCAG AAAAAAGCAT AATCAGTGAC AAAACAGCTG AATGGATCGC CGAAGATGAT
    GATGACGTGT TTGTGGCTTC ACGCACAACC GAAGATTTAT TTACTGTGAT ACACAGGTCC
    AAAAGAAAGC TGCTTGGCTG GAAAGAGCCT GGGGAGGCCT TTGCTGGTGG CAGCAGATCA
    AGCTCCCATT CACCAATAAA GAACACAGCT GATTTTTCCA TCAGTGAGTC AGCTGTCTCT
    GCGGGGTCGA GTGGCAGCGC CAACCTGGAT GCTGGCAGAA ACAATGATTT CAAGGCCTTG
    CTGCAAAAGA AGGGAAGCAA GGCAACCCCC AAGTCCCGCC CCTCAGCTGC TGAACTGCTC
    AAGACCACTA ATCCGCTGGC TCGGAGAATT ATTGCACAAT TTTCCAAAGA GTATGAGACC
    ACCAACAACC CTGGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32540
    Clone ID Related Accession (Same CDS sequence) XM_005641480.3 , XM_005641480.2
    Accession Version XM_005641480.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGAGAG CCGAGTTTGT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGAGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTACT CAGAGGCCAG ACTTCTGGGG
    CAGACCTTCC GCTCTGCTGA TGAGGCCCCC GAGGCCACTC CTAGCCCAAG GCCCCAGGCT
    GGCAGGCGTC TGGAGCTTGT ATTGATGCCT ACAAAACGGC AGCTGAGCGA GGATGAGACT
    ACCACCCAGG GTGTGAGGGC CCCTGAGGCC TCCTCGAGCC TGTCTACCAC AGCCGACAAG
    CAAGCTGCCT GGAATAGCCC CTTCCCTCTG CCCATCCTAG AGGAGAAGCG GTGGCTTCAG
    CCTTGCTCCA CGCAATCTGA CATTGTGCCC ATCAACATCT CTGGGCAGCA GTTTGATAAA
    CATGCAAGTT TGCGACACTC GTTGTTTAAC ACAGAGACAG CTGTGAACCC CAAATCCACC
    CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTTTT CCCAGCGAGA CCAAGGTCAT
    AGCAACAGGC CTGCAGGCAG TGTGGCCCAC TCTGCCACCT CAGACATCAA GTCCAGTCAT
    TCCATCCCAG AAGGGGTTCA TGGAAGAATT GCCATTGGTC AGGAAGCTCA GTTCCCAAAT
    CTCACCTCAC CAGTACTGAG GAGTCCTTCT AGTGATTCAG AAGAACCTCT CCAGGCATGT
    GGTGGCACAA ACCCTCCTGG TATGGAGAGC ATAGGAATGG TGTATAGCAT CCCCAGTTCT
    TGCAATGGAC CAACGGAATC AACGTTCTCT GCTTCCTGGA AGGGAGATAC TTTTACCTAC
    ATGACTCCAA GTGTCACCAG TCAGAACAAT CAAATCAGTC AAAATGGAAG AAATCCTTCC
    TCTGGGAATG CTTGGGTCTC TCTGCACACA CTGCCACCTC TAGTCCCCAA GGAGGCCACT
    ACCTTCTTTG TTACCCGAGA GAACCCAACA GGATGCAGTG GGGCAACTGG CAACTCTGAG
    CATCCTACTC AACGAAGGCA AGTATCAGAG CGACCCTCCA AGATGGGCCT TCTCACCAGT
    GGTACCTCAA GGCTGGAGAC AGGCCGGGGG GGTGCCAGCA GGTTCCGGGA GCGGTCACTG
    TCTGTGCCCA TGGACTCTGG CACCACGGAT GTGGACTATG ATGAGGAACA GAAGGCCAGT
    GAAGCCTGTG CCCTGCCTTA TGCCAGTACA AGCTCTGAGG GCAGTAACAG TACTGACAAC
    ATTGCCTCCC TTAGCACCCA ACAAGAGGCC CAGCCCAGAA GGCAGAGATC CAAGAGTATC
    TCACTCCGGA AGGCCAAGAA GAAGCCTTCC CCACCGATGC GCAGTGTCTC ACTGCTCAAA
    GATGGGCCAG TCCTCTTGCC AGAACATGGG TCAGCGCTGC CCAAGGACCA GAGGCCCAGG
    AGTCTCTGCC TCTCCTTGGA ACACCAAGGG CGTCAGTCAT CCCACCCAGA TGCTCAGGGT
    CACCCAGCTG TGCCAACCGT CAAAGATCCA GAAGGTGCAC AATTCTCCCA CCACTGGTAT
    CCTACTAACT GGAAGTCTGG CGACACCTAC CAATCCTTGT CCAGTTCCAG CACGGCCACT
    GGCACAACAG TCATCGAGTG CACTCAAGTT CAGGGAAGCT CAGAGTCTCT TGCCTCCCCT
    TCCACATCCA GAGCCACGAC ACCTTCCCAA CTCTCTATCG AGGTGGAGGC CAGGGAAGTG
    TCCTCCCCAG GAAGGCCCAC TGGGCTGATG TCACCTTCCA GTGGCTACTC CAGCCAATCA
    GAGACACCAA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACCTGCC CCCTCCAAGT
    GGCAGTGTCC GGGTGCGGCC AGTGGTACCC GAGAGGAAGT CATCACTCCC CCCGACATCG
    CCTATGGAGA AAATCTCCAA GTCCCGGTTG TCCTTTGACC TGCCACTGAC CTCCTCAACC
    AACCTGGATC TGTCTGGGAT GAGCATCTCC ATCCGAAGCA AAACCAAGGT GAGCCGGCAT
    CATTCAGATA CAAATTTTGG GGCTAAGCTG GCCCTGAAAA AGAGCCCCAA CCAGCCATTT
    ATGCCCATGG TCACTCAGTC TGACCTACGT TCCATTCACC TGAGGTCTGT CAGCAAGTCT
    GAGCCGGAAG ATGACATCGA GAGCCCTGAC TATGCTGAGG ACGCAGGAGC AGAGGTCTTC
    GTATTGCTGG AGAGAAAGAT GAAACCTCCC ATAGCCGAGA AGCCCCTTGT GGCCCGAAGT
    CCTCCCAGCT TGGTCCACAA GCCACCATCT GCCCCTGAGG AGTACCCACT CACTTCACCG
    ACCTTGGCTG TGACCCCCAA GATCTCTGTT CAACACATGC GGCCACTCCC CAAAGACATC
    TACACGGTGA TGCGGAAACC AAAGTCCCCC GGCTTCCCCG AGGGCAGAGG CTCCAGGGAG
    CCACCAGCGC CCTCTTCTCT TGCTTTCACA CCTTTTGCCA GTTCCTCTGG TGCTTTCTTC
    TCAGGAACAC AGCAACCTCC CCAGGGCACA CTGGAGGATG GGGGCCCCAA GGTGAGAGCC
    CTGCCTGAAA GAATCAGCCT CCAGAGCCAG GAAGAAGCAG AGAAAAAGAA AGGCAAGATT
    CCACCTCCTG TCCCCAAAAA ACCCAGCGTG CTGTACCTGC CTCTCACATC ACCCACAGCA
    CACATGGAGG TGTTCAGGGC CGAACTGAGG CTGCCCCACA GCCCCATCAT CACCCTGCAG
    GAAGACACCA AGTGTCCGCC CACCAGTGAC CCCCTGAAGG CACCTGGTGT GAGGCCAACT
    TCACCGCTGC AGGCTGAAGG TGAAAGGGAG GCAAGTCCTC TAGGGAGTTC TGTGGAACCG
    AGCACTGCAG AAAAAAGCAT AATCAGTGAC AAAACAGCTG AATGGATCGC CGAAGATGAT
    GATGACGTGT TTGTGGCTTC ACGCACAACC GAAGATTTAT TTACTGTGAT ACACAGGTCC
    AAAAGAAAGC TGCTTGGCTG GAAAGAGCCT GGGGAGGCCT TTGCTGGTGG CAGCAGATCA
    AGCTCCCATT CACCAATAAA GAACACAGCT GATTTTTCCA TCAGTGAGTC AGCTGTCTCT
    GCGGGGTCGA GTGGCAGCGC CAACCTGGAT GCTGGCAGAA ACAATGATTT CAAGGCCTTG
    CTGCAAAAGA AGGGAAGCAA GGCAACCCCC AAGTCCCGCC CCTCAGCTGC TGAACTGCTC
    AAGACCACTA ATCCGCTGGC TCGGAGAATT ATTGCACAAT TTTCCAAAGA GTATGAGACC
    ACCAACAACC CTGGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641537.1
    CDS494..3934
    Translation

    Target ORF information:

    RefSeq Version XM_005641480.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641480.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGAGAG CCGAGTTTGT CACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAGCTGC TTCGAGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTACT CAGAGGCCAG ACTTCTGGGG
    CAGACCTTCC GCTCTGCTGA TGAGGCCCCC GAGGCCACTC CTAGCCCAAG GCCCCAGGCT
    GGCAGGCGTC TGGAGCTTGT ATTGATGCCT ACAAAACGGC AGCTGAGCGA GGATGAGACT
    ACCACCCAGG GTGTGAGGGC CCCTGAGGCC TCCTCGAGCC TGTCTACCAC AGCCGACAAG
    CAAGCTGCCT GGAATAGCCC CTTCCCTCTG CCCATCCTAG AGGAGAAGCG GTGGCTTCAG
    CCTTGCTCCA CGCAATCTGA CATTGTGCCC ATCAACATCT CTGGGCAGCA GTTTGATAAA
    CATGCAAGTT TGCGACACTC GTTGTTTAAC ACAGAGACAG CTGTGAACCC CAAATCCACC
    CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTTTT CCCAGCGAGA CCAAGGTCAT
    AGCAACAGGC CTGCAGGCAG TGTGGCCCAC TCTGCCACCT CAGACATCAA GTCCAGTCAT
    TCCATCCCAG AAGGGGTTCA TGGAAGAATT GCCATTGGTC AGGAAGCTCA GTTCCCAAAT
    CTCACCTCAC CAGTACTGAG GAGTCCTTCT AGTGATTCAG AAGAACCTCT CCAGGCATGT
    GGTGGCACAA ACCCTCCTGG TATGGAGAGC ATAGGAATGG TGTATAGCAT CCCCAGTTCT
    TGCAATGGAC CAACGGAATC AACGTTCTCT GCTTCCTGGA AGGGAGATAC TTTTACCTAC
    ATGACTCCAA GTGTCACCAG TCAGAACAAT CAAATCAGTC AAAATGGAAG AAATCCTTCC
    TCTGGGAATG CTTGGGTCTC TCTGCACACA CTGCCACCTC TAGTCCCCAA GGAGGCCACT
    ACCTTCTTTG TTACCCGAGA GAACCCAACA GGATGCAGTG GGGCAACTGG CAACTCTGAG
    CATCCTACTC AACGAAGGCA AGTATCAGAG CGACCCTCCA AGATGGGCCT TCTCACCAGT
    GGTACCTCAA GGCTGGAGAC AGGCCGGGGG GGTGCCAGCA GGTTCCGGGA GCGGTCACTG
    TCTGTGCCCA TGGACTCTGG CACCACGGAT GTGGACTATG ATGAGGAACA GAAGGCCAGT
    GAAGCCTGTG CCCTGCCTTA TGCCAGTACA AGCTCTGAGG GCAGTAACAG TACTGACAAC
    ATTGCCTCCC TTAGCACCCA ACAAGAGGCC CAGCCCAGAA GGCAGAGATC CAAGAGTATC
    TCACTCCGGA AGGCCAAGAA GAAGCCTTCC CCACCGATGC GCAGTGTCTC ACTGCTCAAA
    GATGGGCCAG TCCTCTTGCC AGAACATGGG TCAGCGCTGC CCAAGGACCA GAGGCCCAGG
    AGTCTCTGCC TCTCCTTGGA ACACCAAGGG CGTCAGTCAT CCCACCCAGA TGCTCAGGGT
    CACCCAGCTG TGCCAACCGT CAAAGATCCA GAAGGTGCAC AATTCTCCCA CCACTGGTAT
    CCTACTAACT GGAAGTCTGG CGACACCTAC CAATCCTTGT CCAGTTCCAG CACGGCCACT
    GGCACAACAG TCATCGAGTG CACTCAAGTT CAGGGAAGCT CAGAGTCTCT TGCCTCCCCT
    TCCACATCCA GAGCCACGAC ACCTTCCCAA CTCTCTATCG AGGTGGAGGC CAGGGAAGTG
    TCCTCCCCAG GAAGGCCCAC TGGGCTGATG TCACCTTCCA GTGGCTACTC CAGCCAATCA
    GAGACACCAA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACCTGCC CCCTCCAAGT
    GGCAGTGTCC GGGTGCGGCC AGTGGTACCC GAGAGGAAGT CATCACTCCC CCCGACATCG
    CCTATGGAGA AAATCTCCAA GTCCCGGTTG TCCTTTGACC TGCCACTGAC CTCCTCAACC
    AACCTGGATC TGTCTGGGAT GAGCATCTCC ATCCGAAGCA AAACCAAGGT GAGCCGGCAT
    CATTCAGATA CAAATTTTGG GGCTAAGCTG GCCCTGAAAA AGAGCCCCAA CCAGCCATTT
    ATGCCCATGG TCACTCAGTC TGACCTACGT TCCATTCACC TGAGGTCTGT CAGCAAGTCT
    GAGCCGGAAG ATGACATCGA GAGCCCTGAC TATGCTGAGG ACGCAGGAGC AGAGGTCTTC
    GTATTGCTGG AGAGAAAGAT GAAACCTCCC ATAGCCGAGA AGCCCCTTGT GGCCCGAAGT
    CCTCCCAGCT TGGTCCACAA GCCACCATCT GCCCCTGAGG AGTACCCACT CACTTCACCG
    ACCTTGGCTG TGACCCCCAA GATCTCTGTT CAACACATGC GGCCACTCCC CAAAGACATC
    TACACGGTGA TGCGGAAACC AAAGTCCCCC GGCTTCCCCG AGGGCAGAGG CTCCAGGGAG
    CCACCAGCGC CCTCTTCTCT TGCTTTCACA CCTTTTGCCA GTTCCTCTGG TGCTTTCTTC
    TCAGGAACAC AGCAACCTCC CCAGGGCACA CTGGAGGATG GGGGCCCCAA GGTGAGAGCC
    CTGCCTGAAA GAATCAGCCT CCAGAGCCAG GAAGAAGCAG AGAAAAAGAA AGGCAAGATT
    CCACCTCCTG TCCCCAAAAA ACCCAGCGTG CTGTACCTGC CTCTCACATC ACCCACAGCA
    CACATGGAGG TGTTCAGGGC CGAACTGAGG CTGCCCCACA GCCCCATCAT CACCCTGCAG
    GAAGACACCA AGTGTCCGCC CACCAGTGAC CCCCTGAAGG CACCTGGTGT GAGGCCAACT
    TCACCGCTGC AGGCTGAAGG TGAAAGGGAG GCAAGTCCTC TAGGGAGTTC TGTGGAACCG
    AGCACTGCAG AAAAAAGCAT AATCAGTGAC AAAACAGCTG AATGGATCGC CGAAGATGAT
    GATGACGTGT TTGTGGCTTC ACGCACAACC GAAGATTTAT TTACTGTGAT ACACAGGTCC
    AAAAGAAAGC TGCTTGGCTG GAAAGAGCCT GGGGAGGCCT TTGCTGGTGG CAGCAGATCA
    AGCTCCCATT CACCAATAAA GAACACAGCT GATTTTTCCA TCAGTGAGTC AGCTGTCTCT
    GCGGGGTCGA GTGGCAGCGC CAACCTGGAT GCTGGCAGAA ACAATGATTT CAAGGCCTTG
    CTGCAAAAGA AGGGAAGCAA GGCAACCCCC AAGTCCCGCC CCTCAGCTGC TGAACTGCTC
    AAGACCACTA ATCCGCTGGC TCGGAGAATT ATTGCACAAT TTTCCAAAGA GTATGAGACC
    ACCAACAACC CTGGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32541
    Clone ID Related Accession (Same CDS sequence) XM_005641481.2 , XM_005641482.2 , XM_022415944.1
    Accession Version XM_005641481.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 2 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTACAA AACGGCAGCT GAGCGAGGAT GAGACTACCA CCCAGGGTGT GAGGGCCCCT 
    GAGGCCTCCT CGAGCCTGTC TACCACAGCC GACAAGCAAG CTGCCTGGAA TAGCCCCTTC
    CCTCTGCCCA TCCTAGAGGA GAAGCGGTGG CTTCAGCCTT GCTCCACGCA ATCTGACATT
    GTGCCCATCA ACATCTCTGG GCAGCAGTTT GATAAACATG CAAGTTTGCG ACACTCGTTG
    TTTAACACAG AGACAGCTGT GAACCCCAAA TCCACCCTGA GGCGGAGGCG GACCATTATT
    GGATTCTCTA ACTTTTCCCA GCGAGACCAA GGTCATAGCA ACAGGCCTGC AGGCAGTGTG
    GCCCACTCTG CCACCTCAGA CATCAAGTCC AGTCATTCCA TCCCAGAAGG GGTTCATGGA
    AGAATTGCCA TTGGTCAGGA AGCTCAGTTC CCAAATCTCA CCTCACCAGT ACTGAGGAGT
    CCTTCTAGTG ATTCAGAAGA ACCTCTCCAG GCATGTGGTG GCACAAACCC TCCTGGTATG
    GAGAGCATAG GAATGGTGTA TAGCATCCCC AGTTCTTGCA ATGGACCAAC GGAATCAACG
    TTCTCTGCTT CCTGGAAGGG AGATACTTTT ACCTACATGA CTCCAAGTGT CACCAGTCAG
    AACAATCAAA TCAGTCAAAA TGGAAGAAAT CCTTCCTCTG GGAATGCTTG GGTCTCTCTG
    CACACACTGC CACCTCTAGT CCCCAAGGAG GCCACTACCT TCTTTGTTAC CCGAGAGAAC
    CCAACAGGAT GCAGTGGGGC AACTGGCAAC TCTGAGCATC CTACTCAACG AAGGCAAGTA
    TCAGAGCGAC CCTCCAAGAT GGGCCTTCTC ACCAGTGGTA CCTCAAGGCT GGAGACAGGC
    CGGGGGGGTG CCAGCAGGTT CCGGGAGCGG TCACTGTCTG TGCCCATGGA CTCTGGCACC
    ACGGATGTGG ACTATGATGA GGAACAGAAG GCCAGTGAAG CCTGTGCCCT GCCTTATGCC
    AGTACAAGCT CTGAGGGCAG TAACAGTACT GACAACATTG CCTCCCTTAG CACCCAACAA
    GAGGCCCAGC CCAGAAGGCA GAGATCCAAG AGTATCTCAC TCCGGAAGGC CAAGAAGAAG
    CCTTCCCCAC CGATGCGCAG TGTCTCACTG CTCAAAGATG GGCCAGTCCT CTTGCCAGAA
    CATGGGTCAG CGCTGCCCAA GGACCAGAGG CCCAGGAGTC TCTGCCTCTC CTTGGAACAC
    CAAGGGCGTC AGTCATCCCA CCCAGATGCT CAGGGTCACC CAGCTGTGCC AACCGTCAAA
    GATCCAGAAG GTGCACAATT CTCCCACCAC TGGTATCCTA CTAACTGGAA GTCTGGCGAC
    ACCTACCAAT CCTTGTCCAG TTCCAGCACG GCCACTGGCA CAACAGTCAT CGAGTGCACT
    CAAGTTCAGG GAAGCTCAGA GTCTCTTGCC TCCCCTTCCA CATCCAGAGC CACGACACCT
    TCCCAACTCT CTATCGAGGT GGAGGCCAGG GAAGTGTCCT CCCCAGGAAG GCCCACTGGG
    CTGATGTCAC CTTCCAGTGG CTACTCCAGC CAATCAGAGA CACCAACACC CACTGTCTCC
    ATGTCCTTGA CCCTGGGCCA CCTGCCCCCT CCAAGTGGCA GTGTCCGGGT GCGGCCAGTG
    GTACCCGAGA GGAAGTCATC ACTCCCCCCG ACATCGCCTA TGGAGAAAAT CTCCAAGTCC
    CGGTTGTCCT TTGACCTGCC ACTGACCTCC TCAACCAACC TGGATCTGTC TGGGATGAGC
    ATCTCCATCC GAAGCAAAAC CAAGGTGAGC CGGCATCATT CAGATACAAA TTTTGGGGCT
    AAGCTGGCCC TGAAAAAGAG CCCCAACCAG CCATTTATGC CCATGGTCAC TCAGTCTGAC
    CTACGTTCCA TTCACCTGAG GTCTGTCAGC AAGTCTGAGC CGGAAGATGA CATCGAGAGC
    CCTGACTATG CTGAGGACGC AGGAGCAGAG GTCTTCGTAT TGCTGGAGAG AAAGATGAAA
    CCTCCCATAG CCGAGAAGCC CCTTGTGGCC CGAAGTCCTC CCAGCTTGGT CCACAAGCCA
    CCATCTGCCC CTGAGGAGTA CCCACTCACT TCACCGACCT TGGCTGTGAC CCCCAAGATC
    TCTGTTCAAC ACATGCGGCC ACTCCCCAAA GACATCTACA CGGTGATGCG GAAACCAAAG
    TCCCCCGGCT TCCCCGAGGG CAGAGGCTCC AGGGAGCCAC CAGCGCCCTC TTCTCTTGCT
    TTCACACCTT TTGCCAGTTC CTCTGGTGCT TTCTTCTCAG GAACACAGCA ACCTCCCCAG
    GGCACACTGG AGGATGGGGG CCCCAAGGTG AGAGCCCTGC CTGAAAGAAT CAGCCTCCAG
    AGCCAGGAAG AAGCAGAGAA AAAGAAAGGC AAGATTCCAC CTCCTGTCCC CAAAAAACCC
    AGCGTGCTGT ACCTGCCTCT CACATCACCC ACAGCACACA TGGAGGTGTT CAGGGCCGAA
    CTGAGGCTGC CCCACAGCCC CATCATCACC CTGCAGGAAG ACACCAAGTG TCCGCCCACC
    AGTGACCCCC TGAAGGCACC TGGTGTGAGG CCAACTTCAC CGCTGCAGGC TGAAGGTGAA
    AGGGAGGCAA GTCCTCTAGG GAGTTCTGTG GAACCGAGCA CTGCAGAAAA AAGCATAATC
    AGTGACAAAA CAGCTGAATG GATCGCCGAA GATGATGATG ACGTGTTTGT GGCTTCACGC
    ACAACCGAAG ATTTATTTAC TGTGATACAC AGGTCCAAAA GAAAGCTGCT TGGCTGGAAA
    GAGCCTGGGG AGGCCTTTGC TGGTGGCAGC AGATCAAGCT CCCATTCACC AATAAAGAAC
    ACAGCTGATT TTTCCATCAG TGAGTCAGCT GTCTCTGCGG GGTCGAGTGG CAGCGCCAAC
    CTGGATGCTG GCAGAAACAA TGATTTCAAG GCCTTGCTGC AAAAGAAGGG AAGCAAGGCA
    ACCCCCAAGT CCCGCCCCTC AGCTGCTGAA CTGCTCAAGA CCACTAATCC GCTGGCTCGG
    AGAATTATTG CACAATTTTC CAAAGAGTAT GAGACCACCA ACAACCCTGG TACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641538.1
    CDS1228..4344
    Translation

    Target ORF information:

    RefSeq Version XM_005641481.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641481.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTACAA AACGGCAGCT GAGCGAGGAT GAGACTACCA CCCAGGGTGT GAGGGCCCCT 
    GAGGCCTCCT CGAGCCTGTC TACCACAGCC GACAAGCAAG CTGCCTGGAA TAGCCCCTTC
    CCTCTGCCCA TCCTAGAGGA GAAGCGGTGG CTTCAGCCTT GCTCCACGCA ATCTGACATT
    GTGCCCATCA ACATCTCTGG GCAGCAGTTT GATAAACATG CAAGTTTGCG ACACTCGTTG
    TTTAACACAG AGACAGCTGT GAACCCCAAA TCCACCCTGA GGCGGAGGCG GACCATTATT
    GGATTCTCTA ACTTTTCCCA GCGAGACCAA GGTCATAGCA ACAGGCCTGC AGGCAGTGTG
    GCCCACTCTG CCACCTCAGA CATCAAGTCC AGTCATTCCA TCCCAGAAGG GGTTCATGGA
    AGAATTGCCA TTGGTCAGGA AGCTCAGTTC CCAAATCTCA CCTCACCAGT ACTGAGGAGT
    CCTTCTAGTG ATTCAGAAGA ACCTCTCCAG GCATGTGGTG GCACAAACCC TCCTGGTATG
    GAGAGCATAG GAATGGTGTA TAGCATCCCC AGTTCTTGCA ATGGACCAAC GGAATCAACG
    TTCTCTGCTT CCTGGAAGGG AGATACTTTT ACCTACATGA CTCCAAGTGT CACCAGTCAG
    AACAATCAAA TCAGTCAAAA TGGAAGAAAT CCTTCCTCTG GGAATGCTTG GGTCTCTCTG
    CACACACTGC CACCTCTAGT CCCCAAGGAG GCCACTACCT TCTTTGTTAC CCGAGAGAAC
    CCAACAGGAT GCAGTGGGGC AACTGGCAAC TCTGAGCATC CTACTCAACG AAGGCAAGTA
    TCAGAGCGAC CCTCCAAGAT GGGCCTTCTC ACCAGTGGTA CCTCAAGGCT GGAGACAGGC
    CGGGGGGGTG CCAGCAGGTT CCGGGAGCGG TCACTGTCTG TGCCCATGGA CTCTGGCACC
    ACGGATGTGG ACTATGATGA GGAACAGAAG GCCAGTGAAG CCTGTGCCCT GCCTTATGCC
    AGTACAAGCT CTGAGGGCAG TAACAGTACT GACAACATTG CCTCCCTTAG CACCCAACAA
    GAGGCCCAGC CCAGAAGGCA GAGATCCAAG AGTATCTCAC TCCGGAAGGC CAAGAAGAAG
    CCTTCCCCAC CGATGCGCAG TGTCTCACTG CTCAAAGATG GGCCAGTCCT CTTGCCAGAA
    CATGGGTCAG CGCTGCCCAA GGACCAGAGG CCCAGGAGTC TCTGCCTCTC CTTGGAACAC
    CAAGGGCGTC AGTCATCCCA CCCAGATGCT CAGGGTCACC CAGCTGTGCC AACCGTCAAA
    GATCCAGAAG GTGCACAATT CTCCCACCAC TGGTATCCTA CTAACTGGAA GTCTGGCGAC
    ACCTACCAAT CCTTGTCCAG TTCCAGCACG GCCACTGGCA CAACAGTCAT CGAGTGCACT
    CAAGTTCAGG GAAGCTCAGA GTCTCTTGCC TCCCCTTCCA CATCCAGAGC CACGACACCT
    TCCCAACTCT CTATCGAGGT GGAGGCCAGG GAAGTGTCCT CCCCAGGAAG GCCCACTGGG
    CTGATGTCAC CTTCCAGTGG CTACTCCAGC CAATCAGAGA CACCAACACC CACTGTCTCC
    ATGTCCTTGA CCCTGGGCCA CCTGCCCCCT CCAAGTGGCA GTGTCCGGGT GCGGCCAGTG
    GTACCCGAGA GGAAGTCATC ACTCCCCCCG ACATCGCCTA TGGAGAAAAT CTCCAAGTCC
    CGGTTGTCCT TTGACCTGCC ACTGACCTCC TCAACCAACC TGGATCTGTC TGGGATGAGC
    ATCTCCATCC GAAGCAAAAC CAAGGTGAGC CGGCATCATT CAGATACAAA TTTTGGGGCT
    AAGCTGGCCC TGAAAAAGAG CCCCAACCAG CCATTTATGC CCATGGTCAC TCAGTCTGAC
    CTACGTTCCA TTCACCTGAG GTCTGTCAGC AAGTCTGAGC CGGAAGATGA CATCGAGAGC
    CCTGACTATG CTGAGGACGC AGGAGCAGAG GTCTTCGTAT TGCTGGAGAG AAAGATGAAA
    CCTCCCATAG CCGAGAAGCC CCTTGTGGCC CGAAGTCCTC CCAGCTTGGT CCACAAGCCA
    CCATCTGCCC CTGAGGAGTA CCCACTCACT TCACCGACCT TGGCTGTGAC CCCCAAGATC
    TCTGTTCAAC ACATGCGGCC ACTCCCCAAA GACATCTACA CGGTGATGCG GAAACCAAAG
    TCCCCCGGCT TCCCCGAGGG CAGAGGCTCC AGGGAGCCAC CAGCGCCCTC TTCTCTTGCT
    TTCACACCTT TTGCCAGTTC CTCTGGTGCT TTCTTCTCAG GAACACAGCA ACCTCCCCAG
    GGCACACTGG AGGATGGGGG CCCCAAGGTG AGAGCCCTGC CTGAAAGAAT CAGCCTCCAG
    AGCCAGGAAG AAGCAGAGAA AAAGAAAGGC AAGATTCCAC CTCCTGTCCC CAAAAAACCC
    AGCGTGCTGT ACCTGCCTCT CACATCACCC ACAGCACACA TGGAGGTGTT CAGGGCCGAA
    CTGAGGCTGC CCCACAGCCC CATCATCACC CTGCAGGAAG ACACCAAGTG TCCGCCCACC
    AGTGACCCCC TGAAGGCACC TGGTGTGAGG CCAACTTCAC CGCTGCAGGC TGAAGGTGAA
    AGGGAGGCAA GTCCTCTAGG GAGTTCTGTG GAACCGAGCA CTGCAGAAAA AAGCATAATC
    AGTGACAAAA CAGCTGAATG GATCGCCGAA GATGATGATG ACGTGTTTGT GGCTTCACGC
    ACAACCGAAG ATTTATTTAC TGTGATACAC AGGTCCAAAA GAAAGCTGCT TGGCTGGAAA
    GAGCCTGGGG AGGCCTTTGC TGGTGGCAGC AGATCAAGCT CCCATTCACC AATAAAGAAC
    ACAGCTGATT TTTCCATCAG TGAGTCAGCT GTCTCTGCGG GGTCGAGTGG CAGCGCCAAC
    CTGGATGCTG GCAGAAACAA TGATTTCAAG GCCTTGCTGC AAAAGAAGGG AAGCAAGGCA
    ACCCCCAAGT CCCGCCCCTC AGCTGCTGAA CTGCTCAAGA CCACTAATCC GCTGGCTCGG
    AGAATTATTG CACAATTTTC CAAAGAGTAT GAGACCACCA ACAACCCTGG TACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32541
    Clone ID Related Accession (Same CDS sequence) XM_005641481.2 , XM_005641482.2 , XM_022415944.1
    Accession Version XM_005641482.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 2 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTACAA AACGGCAGCT GAGCGAGGAT GAGACTACCA CCCAGGGTGT GAGGGCCCCT 
    GAGGCCTCCT CGAGCCTGTC TACCACAGCC GACAAGCAAG CTGCCTGGAA TAGCCCCTTC
    CCTCTGCCCA TCCTAGAGGA GAAGCGGTGG CTTCAGCCTT GCTCCACGCA ATCTGACATT
    GTGCCCATCA ACATCTCTGG GCAGCAGTTT GATAAACATG CAAGTTTGCG ACACTCGTTG
    TTTAACACAG AGACAGCTGT GAACCCCAAA TCCACCCTGA GGCGGAGGCG GACCATTATT
    GGATTCTCTA ACTTTTCCCA GCGAGACCAA GGTCATAGCA ACAGGCCTGC AGGCAGTGTG
    GCCCACTCTG CCACCTCAGA CATCAAGTCC AGTCATTCCA TCCCAGAAGG GGTTCATGGA
    AGAATTGCCA TTGGTCAGGA AGCTCAGTTC CCAAATCTCA CCTCACCAGT ACTGAGGAGT
    CCTTCTAGTG ATTCAGAAGA ACCTCTCCAG GCATGTGGTG GCACAAACCC TCCTGGTATG
    GAGAGCATAG GAATGGTGTA TAGCATCCCC AGTTCTTGCA ATGGACCAAC GGAATCAACG
    TTCTCTGCTT CCTGGAAGGG AGATACTTTT ACCTACATGA CTCCAAGTGT CACCAGTCAG
    AACAATCAAA TCAGTCAAAA TGGAAGAAAT CCTTCCTCTG GGAATGCTTG GGTCTCTCTG
    CACACACTGC CACCTCTAGT CCCCAAGGAG GCCACTACCT TCTTTGTTAC CCGAGAGAAC
    CCAACAGGAT GCAGTGGGGC AACTGGCAAC TCTGAGCATC CTACTCAACG AAGGCAAGTA
    TCAGAGCGAC CCTCCAAGAT GGGCCTTCTC ACCAGTGGTA CCTCAAGGCT GGAGACAGGC
    CGGGGGGGTG CCAGCAGGTT CCGGGAGCGG TCACTGTCTG TGCCCATGGA CTCTGGCACC
    ACGGATGTGG ACTATGATGA GGAACAGAAG GCCAGTGAAG CCTGTGCCCT GCCTTATGCC
    AGTACAAGCT CTGAGGGCAG TAACAGTACT GACAACATTG CCTCCCTTAG CACCCAACAA
    GAGGCCCAGC CCAGAAGGCA GAGATCCAAG AGTATCTCAC TCCGGAAGGC CAAGAAGAAG
    CCTTCCCCAC CGATGCGCAG TGTCTCACTG CTCAAAGATG GGCCAGTCCT CTTGCCAGAA
    CATGGGTCAG CGCTGCCCAA GGACCAGAGG CCCAGGAGTC TCTGCCTCTC CTTGGAACAC
    CAAGGGCGTC AGTCATCCCA CCCAGATGCT CAGGGTCACC CAGCTGTGCC AACCGTCAAA
    GATCCAGAAG GTGCACAATT CTCCCACCAC TGGTATCCTA CTAACTGGAA GTCTGGCGAC
    ACCTACCAAT CCTTGTCCAG TTCCAGCACG GCCACTGGCA CAACAGTCAT CGAGTGCACT
    CAAGTTCAGG GAAGCTCAGA GTCTCTTGCC TCCCCTTCCA CATCCAGAGC CACGACACCT
    TCCCAACTCT CTATCGAGGT GGAGGCCAGG GAAGTGTCCT CCCCAGGAAG GCCCACTGGG
    CTGATGTCAC CTTCCAGTGG CTACTCCAGC CAATCAGAGA CACCAACACC CACTGTCTCC
    ATGTCCTTGA CCCTGGGCCA CCTGCCCCCT CCAAGTGGCA GTGTCCGGGT GCGGCCAGTG
    GTACCCGAGA GGAAGTCATC ACTCCCCCCG ACATCGCCTA TGGAGAAAAT CTCCAAGTCC
    CGGTTGTCCT TTGACCTGCC ACTGACCTCC TCAACCAACC TGGATCTGTC TGGGATGAGC
    ATCTCCATCC GAAGCAAAAC CAAGGTGAGC CGGCATCATT CAGATACAAA TTTTGGGGCT
    AAGCTGGCCC TGAAAAAGAG CCCCAACCAG CCATTTATGC CCATGGTCAC TCAGTCTGAC
    CTACGTTCCA TTCACCTGAG GTCTGTCAGC AAGTCTGAGC CGGAAGATGA CATCGAGAGC
    CCTGACTATG CTGAGGACGC AGGAGCAGAG GTCTTCGTAT TGCTGGAGAG AAAGATGAAA
    CCTCCCATAG CCGAGAAGCC CCTTGTGGCC CGAAGTCCTC CCAGCTTGGT CCACAAGCCA
    CCATCTGCCC CTGAGGAGTA CCCACTCACT TCACCGACCT TGGCTGTGAC CCCCAAGATC
    TCTGTTCAAC ACATGCGGCC ACTCCCCAAA GACATCTACA CGGTGATGCG GAAACCAAAG
    TCCCCCGGCT TCCCCGAGGG CAGAGGCTCC AGGGAGCCAC CAGCGCCCTC TTCTCTTGCT
    TTCACACCTT TTGCCAGTTC CTCTGGTGCT TTCTTCTCAG GAACACAGCA ACCTCCCCAG
    GGCACACTGG AGGATGGGGG CCCCAAGGTG AGAGCCCTGC CTGAAAGAAT CAGCCTCCAG
    AGCCAGGAAG AAGCAGAGAA AAAGAAAGGC AAGATTCCAC CTCCTGTCCC CAAAAAACCC
    AGCGTGCTGT ACCTGCCTCT CACATCACCC ACAGCACACA TGGAGGTGTT CAGGGCCGAA
    CTGAGGCTGC CCCACAGCCC CATCATCACC CTGCAGGAAG ACACCAAGTG TCCGCCCACC
    AGTGACCCCC TGAAGGCACC TGGTGTGAGG CCAACTTCAC CGCTGCAGGC TGAAGGTGAA
    AGGGAGGCAA GTCCTCTAGG GAGTTCTGTG GAACCGAGCA CTGCAGAAAA AAGCATAATC
    AGTGACAAAA CAGCTGAATG GATCGCCGAA GATGATGATG ACGTGTTTGT GGCTTCACGC
    ACAACCGAAG ATTTATTTAC TGTGATACAC AGGTCCAAAA GAAAGCTGCT TGGCTGGAAA
    GAGCCTGGGG AGGCCTTTGC TGGTGGCAGC AGATCAAGCT CCCATTCACC AATAAAGAAC
    ACAGCTGATT TTTCCATCAG TGAGTCAGCT GTCTCTGCGG GGTCGAGTGG CAGCGCCAAC
    CTGGATGCTG GCAGAAACAA TGATTTCAAG GCCTTGCTGC AAAAGAAGGG AAGCAAGGCA
    ACCCCCAAGT CCCGCCCCTC AGCTGCTGAA CTGCTCAAGA CCACTAATCC GCTGGCTCGG
    AGAATTATTG CACAATTTTC CAAAGAGTAT GAGACCACCA ACAACCCTGG TACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641539.1
    CDS1876..4992
    Translation

    Target ORF information:

    RefSeq Version XM_005641482.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641482.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTACAA AACGGCAGCT GAGCGAGGAT GAGACTACCA CCCAGGGTGT GAGGGCCCCT 
    GAGGCCTCCT CGAGCCTGTC TACCACAGCC GACAAGCAAG CTGCCTGGAA TAGCCCCTTC
    CCTCTGCCCA TCCTAGAGGA GAAGCGGTGG CTTCAGCCTT GCTCCACGCA ATCTGACATT
    GTGCCCATCA ACATCTCTGG GCAGCAGTTT GATAAACATG CAAGTTTGCG ACACTCGTTG
    TTTAACACAG AGACAGCTGT GAACCCCAAA TCCACCCTGA GGCGGAGGCG GACCATTATT
    GGATTCTCTA ACTTTTCCCA GCGAGACCAA GGTCATAGCA ACAGGCCTGC AGGCAGTGTG
    GCCCACTCTG CCACCTCAGA CATCAAGTCC AGTCATTCCA TCCCAGAAGG GGTTCATGGA
    AGAATTGCCA TTGGTCAGGA AGCTCAGTTC CCAAATCTCA CCTCACCAGT ACTGAGGAGT
    CCTTCTAGTG ATTCAGAAGA ACCTCTCCAG GCATGTGGTG GCACAAACCC TCCTGGTATG
    GAGAGCATAG GAATGGTGTA TAGCATCCCC AGTTCTTGCA ATGGACCAAC GGAATCAACG
    TTCTCTGCTT CCTGGAAGGG AGATACTTTT ACCTACATGA CTCCAAGTGT CACCAGTCAG
    AACAATCAAA TCAGTCAAAA TGGAAGAAAT CCTTCCTCTG GGAATGCTTG GGTCTCTCTG
    CACACACTGC CACCTCTAGT CCCCAAGGAG GCCACTACCT TCTTTGTTAC CCGAGAGAAC
    CCAACAGGAT GCAGTGGGGC AACTGGCAAC TCTGAGCATC CTACTCAACG AAGGCAAGTA
    TCAGAGCGAC CCTCCAAGAT GGGCCTTCTC ACCAGTGGTA CCTCAAGGCT GGAGACAGGC
    CGGGGGGGTG CCAGCAGGTT CCGGGAGCGG TCACTGTCTG TGCCCATGGA CTCTGGCACC
    ACGGATGTGG ACTATGATGA GGAACAGAAG GCCAGTGAAG CCTGTGCCCT GCCTTATGCC
    AGTACAAGCT CTGAGGGCAG TAACAGTACT GACAACATTG CCTCCCTTAG CACCCAACAA
    GAGGCCCAGC CCAGAAGGCA GAGATCCAAG AGTATCTCAC TCCGGAAGGC CAAGAAGAAG
    CCTTCCCCAC CGATGCGCAG TGTCTCACTG CTCAAAGATG GGCCAGTCCT CTTGCCAGAA
    CATGGGTCAG CGCTGCCCAA GGACCAGAGG CCCAGGAGTC TCTGCCTCTC CTTGGAACAC
    CAAGGGCGTC AGTCATCCCA CCCAGATGCT CAGGGTCACC CAGCTGTGCC AACCGTCAAA
    GATCCAGAAG GTGCACAATT CTCCCACCAC TGGTATCCTA CTAACTGGAA GTCTGGCGAC
    ACCTACCAAT CCTTGTCCAG TTCCAGCACG GCCACTGGCA CAACAGTCAT CGAGTGCACT
    CAAGTTCAGG GAAGCTCAGA GTCTCTTGCC TCCCCTTCCA CATCCAGAGC CACGACACCT
    TCCCAACTCT CTATCGAGGT GGAGGCCAGG GAAGTGTCCT CCCCAGGAAG GCCCACTGGG
    CTGATGTCAC CTTCCAGTGG CTACTCCAGC CAATCAGAGA CACCAACACC CACTGTCTCC
    ATGTCCTTGA CCCTGGGCCA CCTGCCCCCT CCAAGTGGCA GTGTCCGGGT GCGGCCAGTG
    GTACCCGAGA GGAAGTCATC ACTCCCCCCG ACATCGCCTA TGGAGAAAAT CTCCAAGTCC
    CGGTTGTCCT TTGACCTGCC ACTGACCTCC TCAACCAACC TGGATCTGTC TGGGATGAGC
    ATCTCCATCC GAAGCAAAAC CAAGGTGAGC CGGCATCATT CAGATACAAA TTTTGGGGCT
    AAGCTGGCCC TGAAAAAGAG CCCCAACCAG CCATTTATGC CCATGGTCAC TCAGTCTGAC
    CTACGTTCCA TTCACCTGAG GTCTGTCAGC AAGTCTGAGC CGGAAGATGA CATCGAGAGC
    CCTGACTATG CTGAGGACGC AGGAGCAGAG GTCTTCGTAT TGCTGGAGAG AAAGATGAAA
    CCTCCCATAG CCGAGAAGCC CCTTGTGGCC CGAAGTCCTC CCAGCTTGGT CCACAAGCCA
    CCATCTGCCC CTGAGGAGTA CCCACTCACT TCACCGACCT TGGCTGTGAC CCCCAAGATC
    TCTGTTCAAC ACATGCGGCC ACTCCCCAAA GACATCTACA CGGTGATGCG GAAACCAAAG
    TCCCCCGGCT TCCCCGAGGG CAGAGGCTCC AGGGAGCCAC CAGCGCCCTC TTCTCTTGCT
    TTCACACCTT TTGCCAGTTC CTCTGGTGCT TTCTTCTCAG GAACACAGCA ACCTCCCCAG
    GGCACACTGG AGGATGGGGG CCCCAAGGTG AGAGCCCTGC CTGAAAGAAT CAGCCTCCAG
    AGCCAGGAAG AAGCAGAGAA AAAGAAAGGC AAGATTCCAC CTCCTGTCCC CAAAAAACCC
    AGCGTGCTGT ACCTGCCTCT CACATCACCC ACAGCACACA TGGAGGTGTT CAGGGCCGAA
    CTGAGGCTGC CCCACAGCCC CATCATCACC CTGCAGGAAG ACACCAAGTG TCCGCCCACC
    AGTGACCCCC TGAAGGCACC TGGTGTGAGG CCAACTTCAC CGCTGCAGGC TGAAGGTGAA
    AGGGAGGCAA GTCCTCTAGG GAGTTCTGTG GAACCGAGCA CTGCAGAAAA AAGCATAATC
    AGTGACAAAA CAGCTGAATG GATCGCCGAA GATGATGATG ACGTGTTTGT GGCTTCACGC
    ACAACCGAAG ATTTATTTAC TGTGATACAC AGGTCCAAAA GAAAGCTGCT TGGCTGGAAA
    GAGCCTGGGG AGGCCTTTGC TGGTGGCAGC AGATCAAGCT CCCATTCACC AATAAAGAAC
    ACAGCTGATT TTTCCATCAG TGAGTCAGCT GTCTCTGCGG GGTCGAGTGG CAGCGCCAAC
    CTGGATGCTG GCAGAAACAA TGATTTCAAG GCCTTGCTGC AAAAGAAGGG AAGCAAGGCA
    ACCCCCAAGT CCCGCCCCTC AGCTGCTGAA CTGCTCAAGA CCACTAATCC GCTGGCTCGG
    AGAATTATTG CACAATTTTC CAAAGAGTAT GAGACCACCA ACAACCCTGG TACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32541
    Clone ID Related Accession (Same CDS sequence) XM_005641481.2 , XM_005641482.2 , XM_022415944.1
    Accession Version XM_022415944.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NHS-like protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTACAA AACGGCAGCT GAGCGAGGAT GAGACTACCA CCCAGGGTGT GAGGGCCCCT 
    GAGGCCTCCT CGAGCCTGTC TACCACAGCC GACAAGCAAG CTGCCTGGAA TAGCCCCTTC
    CCTCTGCCCA TCCTAGAGGA GAAGCGGTGG CTTCAGCCTT GCTCCACGCA ATCTGACATT
    GTGCCCATCA ACATCTCTGG GCAGCAGTTT GATAAACATG CAAGTTTGCG ACACTCGTTG
    TTTAACACAG AGACAGCTGT GAACCCCAAA TCCACCCTGA GGCGGAGGCG GACCATTATT
    GGATTCTCTA ACTTTTCCCA GCGAGACCAA GGTCATAGCA ACAGGCCTGC AGGCAGTGTG
    GCCCACTCTG CCACCTCAGA CATCAAGTCC AGTCATTCCA TCCCAGAAGG GGTTCATGGA
    AGAATTGCCA TTGGTCAGGA AGCTCAGTTC CCAAATCTCA CCTCACCAGT ACTGAGGAGT
    CCTTCTAGTG ATTCAGAAGA ACCTCTCCAG GCATGTGGTG GCACAAACCC TCCTGGTATG
    GAGAGCATAG GAATGGTGTA TAGCATCCCC AGTTCTTGCA ATGGACCAAC GGAATCAACG
    TTCTCTGCTT CCTGGAAGGG AGATACTTTT ACCTACATGA CTCCAAGTGT CACCAGTCAG
    AACAATCAAA TCAGTCAAAA TGGAAGAAAT CCTTCCTCTG GGAATGCTTG GGTCTCTCTG
    CACACACTGC CACCTCTAGT CCCCAAGGAG GCCACTACCT TCTTTGTTAC CCGAGAGAAC
    CCAACAGGAT GCAGTGGGGC AACTGGCAAC TCTGAGCATC CTACTCAACG AAGGCAAGTA
    TCAGAGCGAC CCTCCAAGAT GGGCCTTCTC ACCAGTGGTA CCTCAAGGCT GGAGACAGGC
    CGGGGGGGTG CCAGCAGGTT CCGGGAGCGG TCACTGTCTG TGCCCATGGA CTCTGGCACC
    ACGGATGTGG ACTATGATGA GGAACAGAAG GCCAGTGAAG CCTGTGCCCT GCCTTATGCC
    AGTACAAGCT CTGAGGGCAG TAACAGTACT GACAACATTG CCTCCCTTAG CACCCAACAA
    GAGGCCCAGC CCAGAAGGCA GAGATCCAAG AGTATCTCAC TCCGGAAGGC CAAGAAGAAG
    CCTTCCCCAC CGATGCGCAG TGTCTCACTG CTCAAAGATG GGCCAGTCCT CTTGCCAGAA
    CATGGGTCAG CGCTGCCCAA GGACCAGAGG CCCAGGAGTC TCTGCCTCTC CTTGGAACAC
    CAAGGGCGTC AGTCATCCCA CCCAGATGCT CAGGGTCACC CAGCTGTGCC AACCGTCAAA
    GATCCAGAAG GTGCACAATT CTCCCACCAC TGGTATCCTA CTAACTGGAA GTCTGGCGAC
    ACCTACCAAT CCTTGTCCAG TTCCAGCACG GCCACTGGCA CAACAGTCAT CGAGTGCACT
    CAAGTTCAGG GAAGCTCAGA GTCTCTTGCC TCCCCTTCCA CATCCAGAGC CACGACACCT
    TCCCAACTCT CTATCGAGGT GGAGGCCAGG GAAGTGTCCT CCCCAGGAAG GCCCACTGGG
    CTGATGTCAC CTTCCAGTGG CTACTCCAGC CAATCAGAGA CACCAACACC CACTGTCTCC
    ATGTCCTTGA CCCTGGGCCA CCTGCCCCCT CCAAGTGGCA GTGTCCGGGT GCGGCCAGTG
    GTACCCGAGA GGAAGTCATC ACTCCCCCCG ACATCGCCTA TGGAGAAAAT CTCCAAGTCC
    CGGTTGTCCT TTGACCTGCC ACTGACCTCC TCAACCAACC TGGATCTGTC TGGGATGAGC
    ATCTCCATCC GAAGCAAAAC CAAGGTGAGC CGGCATCATT CAGATACAAA TTTTGGGGCT
    AAGCTGGCCC TGAAAAAGAG CCCCAACCAG CCATTTATGC CCATGGTCAC TCAGTCTGAC
    CTACGTTCCA TTCACCTGAG GTCTGTCAGC AAGTCTGAGC CGGAAGATGA CATCGAGAGC
    CCTGACTATG CTGAGGACGC AGGAGCAGAG GTCTTCGTAT TGCTGGAGAG AAAGATGAAA
    CCTCCCATAG CCGAGAAGCC CCTTGTGGCC CGAAGTCCTC CCAGCTTGGT CCACAAGCCA
    CCATCTGCCC CTGAGGAGTA CCCACTCACT TCACCGACCT TGGCTGTGAC CCCCAAGATC
    TCTGTTCAAC ACATGCGGCC ACTCCCCAAA GACATCTACA CGGTGATGCG GAAACCAAAG
    TCCCCCGGCT TCCCCGAGGG CAGAGGCTCC AGGGAGCCAC CAGCGCCCTC TTCTCTTGCT
    TTCACACCTT TTGCCAGTTC CTCTGGTGCT TTCTTCTCAG GAACACAGCA ACCTCCCCAG
    GGCACACTGG AGGATGGGGG CCCCAAGGTG AGAGCCCTGC CTGAAAGAAT CAGCCTCCAG
    AGCCAGGAAG AAGCAGAGAA AAAGAAAGGC AAGATTCCAC CTCCTGTCCC CAAAAAACCC
    AGCGTGCTGT ACCTGCCTCT CACATCACCC ACAGCACACA TGGAGGTGTT CAGGGCCGAA
    CTGAGGCTGC CCCACAGCCC CATCATCACC CTGCAGGAAG ACACCAAGTG TCCGCCCACC
    AGTGACCCCC TGAAGGCACC TGGTGTGAGG CCAACTTCAC CGCTGCAGGC TGAAGGTGAA
    AGGGAGGCAA GTCCTCTAGG GAGTTCTGTG GAACCGAGCA CTGCAGAAAA AAGCATAATC
    AGTGACAAAA CAGCTGAATG GATCGCCGAA GATGATGATG ACGTGTTTGT GGCTTCACGC
    ACAACCGAAG ATTTATTTAC TGTGATACAC AGGTCCAAAA GAAAGCTGCT TGGCTGGAAA
    GAGCCTGGGG AGGCCTTTGC TGGTGGCAGC AGATCAAGCT CCCATTCACC AATAAAGAAC
    ACAGCTGATT TTTCCATCAG TGAGTCAGCT GTCTCTGCGG GGTCGAGTGG CAGCGCCAAC
    CTGGATGCTG GCAGAAACAA TGATTTCAAG GCCTTGCTGC AAAAGAAGGG AAGCAAGGCA
    ACCCCCAAGT CCCGCCCCTC AGCTGCTGAA CTGCTCAAGA CCACTAATCC GCTGGCTCGG
    AGAATTATTG CACAATTTTC CAAAGAGTAT GAGACCACCA ACAACCCTGG TACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271652.1
    CDS833..3949
    Translation

    Target ORF information:

    RefSeq Version XM_022415944.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NHS like 2 (LOC491954), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415944.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTACAA AACGGCAGCT GAGCGAGGAT GAGACTACCA CCCAGGGTGT GAGGGCCCCT 
    GAGGCCTCCT CGAGCCTGTC TACCACAGCC GACAAGCAAG CTGCCTGGAA TAGCCCCTTC
    CCTCTGCCCA TCCTAGAGGA GAAGCGGTGG CTTCAGCCTT GCTCCACGCA ATCTGACATT
    GTGCCCATCA ACATCTCTGG GCAGCAGTTT GATAAACATG CAAGTTTGCG ACACTCGTTG
    TTTAACACAG AGACAGCTGT GAACCCCAAA TCCACCCTGA GGCGGAGGCG GACCATTATT
    GGATTCTCTA ACTTTTCCCA GCGAGACCAA GGTCATAGCA ACAGGCCTGC AGGCAGTGTG
    GCCCACTCTG CCACCTCAGA CATCAAGTCC AGTCATTCCA TCCCAGAAGG GGTTCATGGA
    AGAATTGCCA TTGGTCAGGA AGCTCAGTTC CCAAATCTCA CCTCACCAGT ACTGAGGAGT
    CCTTCTAGTG ATTCAGAAGA ACCTCTCCAG GCATGTGGTG GCACAAACCC TCCTGGTATG
    GAGAGCATAG GAATGGTGTA TAGCATCCCC AGTTCTTGCA ATGGACCAAC GGAATCAACG
    TTCTCTGCTT CCTGGAAGGG AGATACTTTT ACCTACATGA CTCCAAGTGT CACCAGTCAG
    AACAATCAAA TCAGTCAAAA TGGAAGAAAT CCTTCCTCTG GGAATGCTTG GGTCTCTCTG
    CACACACTGC CACCTCTAGT CCCCAAGGAG GCCACTACCT TCTTTGTTAC CCGAGAGAAC
    CCAACAGGAT GCAGTGGGGC AACTGGCAAC TCTGAGCATC CTACTCAACG AAGGCAAGTA
    TCAGAGCGAC CCTCCAAGAT GGGCCTTCTC ACCAGTGGTA CCTCAAGGCT GGAGACAGGC
    CGGGGGGGTG CCAGCAGGTT CCGGGAGCGG TCACTGTCTG TGCCCATGGA CTCTGGCACC
    ACGGATGTGG ACTATGATGA GGAACAGAAG GCCAGTGAAG CCTGTGCCCT GCCTTATGCC
    AGTACAAGCT CTGAGGGCAG TAACAGTACT GACAACATTG CCTCCCTTAG CACCCAACAA
    GAGGCCCAGC CCAGAAGGCA GAGATCCAAG AGTATCTCAC TCCGGAAGGC CAAGAAGAAG
    CCTTCCCCAC CGATGCGCAG TGTCTCACTG CTCAAAGATG GGCCAGTCCT CTTGCCAGAA
    CATGGGTCAG CGCTGCCCAA GGACCAGAGG CCCAGGAGTC TCTGCCTCTC CTTGGAACAC
    CAAGGGCGTC AGTCATCCCA CCCAGATGCT CAGGGTCACC CAGCTGTGCC AACCGTCAAA
    GATCCAGAAG GTGCACAATT CTCCCACCAC TGGTATCCTA CTAACTGGAA GTCTGGCGAC
    ACCTACCAAT CCTTGTCCAG TTCCAGCACG GCCACTGGCA CAACAGTCAT CGAGTGCACT
    CAAGTTCAGG GAAGCTCAGA GTCTCTTGCC TCCCCTTCCA CATCCAGAGC CACGACACCT
    TCCCAACTCT CTATCGAGGT GGAGGCCAGG GAAGTGTCCT CCCCAGGAAG GCCCACTGGG
    CTGATGTCAC CTTCCAGTGG CTACTCCAGC CAATCAGAGA CACCAACACC CACTGTCTCC
    ATGTCCTTGA CCCTGGGCCA CCTGCCCCCT CCAAGTGGCA GTGTCCGGGT GCGGCCAGTG
    GTACCCGAGA GGAAGTCATC ACTCCCCCCG ACATCGCCTA TGGAGAAAAT CTCCAAGTCC
    CGGTTGTCCT TTGACCTGCC ACTGACCTCC TCAACCAACC TGGATCTGTC TGGGATGAGC
    ATCTCCATCC GAAGCAAAAC CAAGGTGAGC CGGCATCATT CAGATACAAA TTTTGGGGCT
    AAGCTGGCCC TGAAAAAGAG CCCCAACCAG CCATTTATGC CCATGGTCAC TCAGTCTGAC
    CTACGTTCCA TTCACCTGAG GTCTGTCAGC AAGTCTGAGC CGGAAGATGA CATCGAGAGC
    CCTGACTATG CTGAGGACGC AGGAGCAGAG GTCTTCGTAT TGCTGGAGAG AAAGATGAAA
    CCTCCCATAG CCGAGAAGCC CCTTGTGGCC CGAAGTCCTC CCAGCTTGGT CCACAAGCCA
    CCATCTGCCC CTGAGGAGTA CCCACTCACT TCACCGACCT TGGCTGTGAC CCCCAAGATC
    TCTGTTCAAC ACATGCGGCC ACTCCCCAAA GACATCTACA CGGTGATGCG GAAACCAAAG
    TCCCCCGGCT TCCCCGAGGG CAGAGGCTCC AGGGAGCCAC CAGCGCCCTC TTCTCTTGCT
    TTCACACCTT TTGCCAGTTC CTCTGGTGCT TTCTTCTCAG GAACACAGCA ACCTCCCCAG
    GGCACACTGG AGGATGGGGG CCCCAAGGTG AGAGCCCTGC CTGAAAGAAT CAGCCTCCAG
    AGCCAGGAAG AAGCAGAGAA AAAGAAAGGC AAGATTCCAC CTCCTGTCCC CAAAAAACCC
    AGCGTGCTGT ACCTGCCTCT CACATCACCC ACAGCACACA TGGAGGTGTT CAGGGCCGAA
    CTGAGGCTGC CCCACAGCCC CATCATCACC CTGCAGGAAG ACACCAAGTG TCCGCCCACC
    AGTGACCCCC TGAAGGCACC TGGTGTGAGG CCAACTTCAC CGCTGCAGGC TGAAGGTGAA
    AGGGAGGCAA GTCCTCTAGG GAGTTCTGTG GAACCGAGCA CTGCAGAAAA AAGCATAATC
    AGTGACAAAA CAGCTGAATG GATCGCCGAA GATGATGATG ACGTGTTTGT GGCTTCACGC
    ACAACCGAAG ATTTATTTAC TGTGATACAC AGGTCCAAAA GAAAGCTGCT TGGCTGGAAA
    GAGCCTGGGG AGGCCTTTGC TGGTGGCAGC AGATCAAGCT CCCATTCACC AATAAAGAAC
    ACAGCTGATT TTTCCATCAG TGAGTCAGCT GTCTCTGCGG GGTCGAGTGG CAGCGCCAAC
    CTGGATGCTG GCAGAAACAA TGATTTCAAG GCCTTGCTGC AAAAGAAGGG AAGCAAGGCA
    ACCCCCAAGT CCCGCCCCTC AGCTGCTGAA CTGCTCAAGA CCACTAATCC GCTGGCTCGG
    AGAATTATTG CACAATTTTC CAAAGAGTAT GAGACCACCA ACAACCCTGG TACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32542
    Clone ID Related Accession (Same CDS sequence) XM_005641483.2
    Accession Version XM_005641483.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3066bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NHS-like protein 2 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGCAA CCCGCCGCAG 
    CCGCTCACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCCTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GCTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAA CCTGTTCCAG CGCACTGTGC GCCTCCGCCG CCGCCTTCCC
    TGCCACCTCC TCGGCCTGGA GGACGATGAG GAAGAGCTAG GTCATAGCAA CAGGCCTGCA
    GGCAGTGTGG CCCACTCTGC CACCTCAGAC ATCAAGTCCA GTCATTCCAT CCCAGAAGGG
    GTTCATGGAA GAATTGCCAT TGGTCAGGAA GCTCAGTTCC CAAATCTCAC CTCACCAGTA
    CTGAGGAGTC CTTCTAGTGA TTCAGAAGAA CCTCTCCAGG CATGTGGTGG CACAAACCCT
    CCTGGTATGG AGAGCATAGG AATGGTGTAT AGCATCCCCA GTTCTTGCAA TGGACCAACG
    GAATCAACGT TCTCTGCTTC CTGGAAGGGA GATACTTTTA CCTACATGAC TCCAAGTGTC
    ACCAGTCAGA ACAATCAAAT CAGTCAAAAT GGAAGAAATC CTTCCTCTGG GAATGCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTAGTC CCCAAGGAGG CCACTACCTT CTTTGTTACC
    CGAGAGAACC CAACAGGATG CAGTGGGGCA ACTGGCAACT CTGAGCATCC TACTCAACGA
    AGGCAAGTAT CAGAGCGACC CTCCAAGATG GGCCTTCTCA CCAGTGGTAC CTCAAGGCTG
    GAGACAGGCC GGGGGGGTGC CAGCAGGTTC CGGGAGCGGT CACTGTCTGT GCCCATGGAC
    TCTGGCACCA CGGATGTGGA CTATGATGAG GAACAGAAGG CCAGTGAAGC CTGTGCCCTG
    CCTTATGCCA GTACAAGCTC TGAGGGCAGT AACAGTACTG ACAACATTGC CTCCCTTAGC
    ACCCAACAAG AGGCCCAGCC CAGAAGGCAG AGATCCAAGA GTATCTCACT CCGGAAGGCC
    AAGAAGAAGC CTTCCCCACC GATGCGCAGT GTCTCACTGC TCAAAGATGG GCCAGTCCTC
    TTGCCAGAAC ATGGGTCAGC GCTGCCCAAG GACCAGAGGC CCAGGAGTCT CTGCCTCTCC
    TTGGAACACC AAGGGCGTCA GTCATCCCAC CCAGATGCTC AGGGTCACCC AGCTGTGCCA
    ACCGTCAAAG ATCCAGAAGG TGCACAATTC TCCCACCACT GGTATCCTAC TAACTGGAAG
    TCTGGCGACA CCTACCAATC CTTGTCCAGT TCCAGCACGG CCACTGGCAC AACAGTCATC
    GAGTGCACTC AAGTTCAGGG AAGCTCAGAG TCTCTTGCCT CCCCTTCCAC ATCCAGAGCC
    ACGACACCTT CCCAACTCTC TATCGAGGTG GAGGCCAGGG AAGTGTCCTC CCCAGGAAGG
    CCCACTGGGC TGATGTCACC TTCCAGTGGC TACTCCAGCC AATCAGAGAC ACCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC CTGCCCCCTC CAAGTGGCAG TGTCCGGGTG
    CGGCCAGTGG TACCCGAGAG GAAGTCATCA CTCCCCCCGA CATCGCCTAT GGAGAAAATC
    TCCAAGTCCC GGTTGTCCTT TGACCTGCCA CTGACCTCCT CAACCAACCT GGATCTGTCT
    GGGATGAGCA TCTCCATCCG AAGCAAAACC AAGGTGAGCC GGCATCATTC AGATACAAAT
    TTTGGGGCTA AGCTGGCCCT GAAAAAGAGC CCCAACCAGC CATTTATGCC CATGGTCACT
    CAGTCTGACC TACGTTCCAT TCACCTGAGG TCTGTCAGCA AGTCTGAGCC GGAAGATGAC
    ATCGAGAGCC CTGACTATGC TGAGGACGCA GGAGCAGAGG TCTTCGTATT GCTGGAGAGA
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CTTGTGGCCC GAAGTCCTCC CAGCTTGGTC
    CACAAGCCAC CATCTGCCCC TGAGGAGTAC CCACTCACTT CACCGACCTT GGCTGTGACC
    CCCAAGATCT CTGTTCAACA CATGCGGCCA CTCCCCAAAG ACATCTACAC GGTGATGCGG
    AAACCAAAGT CCCCCGGCTT CCCCGAGGGC AGAGGCTCCA GGGAGCCACC AGCGCCCTCT
    TCTCTTGCTT TCACACCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GCACACTGGA GGATGGGGGC CCCAAGGTGA GAGCCCTGCC TGAAAGAATC
    AGCCTCCAGA GCCAGGAAGA AGCAGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTCCCC
    AAAAAACCCA GCGTGCTGTA CCTGCCTCTC ACATCACCCA CAGCACACAT GGAGGTGTTC
    AGGGCCGAAC TGAGGCTGCC CCACAGCCCC ATCATCACCC TGCAGGAAGA CACCAAGTGT
    CCGCCCACCA GTGACCCCCT GAAGGCACCT GGTGTGAGGC CAACTTCACC GCTGCAGGCT
    GAAGGTGAAA GGGAGGCAAG TCCTCTAGGG AGTTCTGTGG AACCGAGCAC TGCAGAAAAA
    AGCATAATCA GTGACAAAAC AGCTGAATGG ATCGCCGAAG ATGATGATGA CGTGTTTGTG
    GCTTCACGCA CAACCGAAGA TTTATTTACT GTGATACACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAAG AGCCTGGGGA GGCCTTTGCT GGTGGCAGCA GATCAAGCTC CCATTCACCA
    ATAAAGAACA CAGCTGATTT TTCCATCAGT GAGTCAGCTG TCTCTGCGGG GTCGAGTGGC
    AGCGCCAACC TGGATGCTGG CAGAAACAAT GATTTCAAGG CCTTGCTGCA AAAGAAGGGA
    AGCAAGGCAA CCCCCAAGTC CCGCCCCTCA GCTGCTGAAC TGCTCAAGAC CACTAATCCG
    CTGGCTCGGA GAATTATTGC ACAATTTTCC AAAGAGTATG AGACCACCAA CAACCCTGGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641540.1
    CDS379..3444
    Translation

    Target ORF information:

    RefSeq Version XM_005641483.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NHS-like 2 (NHSL2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641483.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCGTTCT ACAGGCGCAC GGTGGTGCCC CAGCGCCTGT GCCCGCGCAA CCCGCCGCAG 
    CCGCTCACCG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGCCTGCT CCGGCAGCTC
    GCCGACCTCT GCGGCCACTC GCTGGCTCTG CTCGAGGACC TCGAGGGGCA CCTGCTGGCC
    CTGGGGCGCC GCACCGACAA CCTGTTCCAG CGCACTGTGC GCCTCCGCCG CCGCCTTCCC
    TGCCACCTCC TCGGCCTGGA GGACGATGAG GAAGAGCTAG GTCATAGCAA CAGGCCTGCA
    GGCAGTGTGG CCCACTCTGC CACCTCAGAC ATCAAGTCCA GTCATTCCAT CCCAGAAGGG
    GTTCATGGAA GAATTGCCAT TGGTCAGGAA GCTCAGTTCC CAAATCTCAC CTCACCAGTA
    CTGAGGAGTC CTTCTAGTGA TTCAGAAGAA CCTCTCCAGG CATGTGGTGG CACAAACCCT
    CCTGGTATGG AGAGCATAGG AATGGTGTAT AGCATCCCCA GTTCTTGCAA TGGACCAACG
    GAATCAACGT TCTCTGCTTC CTGGAAGGGA GATACTTTTA CCTACATGAC TCCAAGTGTC
    ACCAGTCAGA ACAATCAAAT CAGTCAAAAT GGAAGAAATC CTTCCTCTGG GAATGCTTGG
    GTCTCTCTGC ACACACTGCC ACCTCTAGTC CCCAAGGAGG CCACTACCTT CTTTGTTACC
    CGAGAGAACC CAACAGGATG CAGTGGGGCA ACTGGCAACT CTGAGCATCC TACTCAACGA
    AGGCAAGTAT CAGAGCGACC CTCCAAGATG GGCCTTCTCA CCAGTGGTAC CTCAAGGCTG
    GAGACAGGCC GGGGGGGTGC CAGCAGGTTC CGGGAGCGGT CACTGTCTGT GCCCATGGAC
    TCTGGCACCA CGGATGTGGA CTATGATGAG GAACAGAAGG CCAGTGAAGC CTGTGCCCTG
    CCTTATGCCA GTACAAGCTC TGAGGGCAGT AACAGTACTG ACAACATTGC CTCCCTTAGC
    ACCCAACAAG AGGCCCAGCC CAGAAGGCAG AGATCCAAGA GTATCTCACT CCGGAAGGCC
    AAGAAGAAGC CTTCCCCACC GATGCGCAGT GTCTCACTGC TCAAAGATGG GCCAGTCCTC
    TTGCCAGAAC ATGGGTCAGC GCTGCCCAAG GACCAGAGGC CCAGGAGTCT CTGCCTCTCC
    TTGGAACACC AAGGGCGTCA GTCATCCCAC CCAGATGCTC AGGGTCACCC AGCTGTGCCA
    ACCGTCAAAG ATCCAGAAGG TGCACAATTC TCCCACCACT GGTATCCTAC TAACTGGAAG
    TCTGGCGACA CCTACCAATC CTTGTCCAGT TCCAGCACGG CCACTGGCAC AACAGTCATC
    GAGTGCACTC AAGTTCAGGG AAGCTCAGAG TCTCTTGCCT CCCCTTCCAC ATCCAGAGCC
    ACGACACCTT CCCAACTCTC TATCGAGGTG GAGGCCAGGG AAGTGTCCTC CCCAGGAAGG
    CCCACTGGGC TGATGTCACC TTCCAGTGGC TACTCCAGCC AATCAGAGAC ACCAACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC CTGCCCCCTC CAAGTGGCAG TGTCCGGGTG
    CGGCCAGTGG TACCCGAGAG GAAGTCATCA CTCCCCCCGA CATCGCCTAT GGAGAAAATC
    TCCAAGTCCC GGTTGTCCTT TGACCTGCCA CTGACCTCCT CAACCAACCT GGATCTGTCT
    GGGATGAGCA TCTCCATCCG AAGCAAAACC AAGGTGAGCC GGCATCATTC AGATACAAAT
    TTTGGGGCTA AGCTGGCCCT GAAAAAGAGC CCCAACCAGC CATTTATGCC CATGGTCACT
    CAGTCTGACC TACGTTCCAT TCACCTGAGG TCTGTCAGCA AGTCTGAGCC GGAAGATGAC
    ATCGAGAGCC CTGACTATGC TGAGGACGCA GGAGCAGAGG TCTTCGTATT GCTGGAGAGA
    AAGATGAAAC CTCCCATAGC CGAGAAGCCC CTTGTGGCCC GAAGTCCTCC CAGCTTGGTC
    CACAAGCCAC CATCTGCCCC TGAGGAGTAC CCACTCACTT CACCGACCTT GGCTGTGACC
    CCCAAGATCT CTGTTCAACA CATGCGGCCA CTCCCCAAAG ACATCTACAC GGTGATGCGG
    AAACCAAAGT CCCCCGGCTT CCCCGAGGGC AGAGGCTCCA GGGAGCCACC AGCGCCCTCT
    TCTCTTGCTT TCACACCTTT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG GCACACTGGA GGATGGGGGC CCCAAGGTGA GAGCCCTGCC TGAAAGAATC
    AGCCTCCAGA GCCAGGAAGA AGCAGAGAAA AAGAAAGGCA AGATTCCACC TCCTGTCCCC
    AAAAAACCCA GCGTGCTGTA CCTGCCTCTC ACATCACCCA CAGCACACAT GGAGGTGTTC
    AGGGCCGAAC TGAGGCTGCC CCACAGCCCC ATCATCACCC TGCAGGAAGA CACCAAGTGT
    CCGCCCACCA GTGACCCCCT GAAGGCACCT GGTGTGAGGC CAACTTCACC GCTGCAGGCT
    GAAGGTGAAA GGGAGGCAAG TCCTCTAGGG AGTTCTGTGG AACCGAGCAC TGCAGAAAAA
    AGCATAATCA GTGACAAAAC AGCTGAATGG ATCGCCGAAG ATGATGATGA CGTGTTTGTG
    GCTTCACGCA CAACCGAAGA TTTATTTACT GTGATACACA GGTCCAAAAG AAAGCTGCTT
    GGCTGGAAAG AGCCTGGGGA GGCCTTTGCT GGTGGCAGCA GATCAAGCTC CCATTCACCA
    ATAAAGAACA CAGCTGATTT TTCCATCAGT GAGTCAGCTG TCTCTGCGGG GTCGAGTGGC
    AGCGCCAACC TGGATGCTGG CAGAAACAAT GATTTCAAGG CCTTGCTGCA AAAGAAGGGA
    AGCAAGGCAA CCCCCAAGTC CCGCCCCTCA GCTGCTGAAC TGCTCAAGAC CACTAATCCG
    CTGGCTCGGA GAATTATTGC ACAATTTTCC AAAGAGTATG AGACCACCAA CAACCCTGGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.