NLRP3 cDNA ORF clone, Canis lupus familiaris(Dog)

The following NLRP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe10057 XM_005623150.3
Latest version!
Canis lupus familiaris NLR family pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe37742 XM_014115621.1
Latest version!
Canis lupus familiaris NLR family, pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe10054 XM_843284.5
Latest version!
Canis lupus familiaris NLR family pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe10055 XM_005623148.3
Latest version!
Canis lupus familiaris NLR family pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe10056 XM_005623149.3
Latest version!
Canis lupus familiaris NLR family pyrin domain containing 3 (NLRP3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe10054 XM_843284.4 Canis lupus familiaris NLR family, pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe10055 XM_005623148.2 Canis lupus familiaris NLR family, pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe10056 XM_005623149.2 Canis lupus familiaris NLR family, pyrin domain containing 3 (NLRP3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe10057 XM_005623150.2 Canis lupus familiaris NLR family, pyrin domain containing 3 (NLRP3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe10057
    Clone ID Related Accession (Same CDS sequence) XM_005623150.3 , XM_005623150.2
    Accession Version XM_005623150.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623150.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGT GAATTCTGGC
    CTCACACCAG GTTGCTGTCC AGCTCTGGCC TCCATGCTCA GCACTAACCA GAAGCTCACA
    CACCTTTACC TACGGGGCAA TGCTCTTGGA GACACAGGGG TGAAGCTACT CTGTGAGGGC
    CTCTTGCACC CCAACTGCAA GCTTCAGATA TTAGAATTGG ATGGCTGTAG CCTCACCTCA
    CACTGTTGCT GGGATCTGTC CACACTTCTG ACCTCCAGCA AGAGCCTCCG GGAGCTAAGC
    CTGGGCAGCA ATGACCTGGG AGATCTAGGG GTCATGCTGC TCTGCGAAGT GCTAAAACAA
    CAGGGCTGCA TCCTACAAAG CCTGAAGTTG TGTGAAATGT ATTTCAATTA TGATACAAAA
    TGTGCATTAG AAACACTTCA AGAAGAAAAG CCTGAGCTGA CCATTGTCTT TGAGCCTTTT
    GGTAGTGACA GAAGCAGGCT GCCAGATGCC AGCGTTTGGG CTCCCTGCCA TCTAGGTGTC
    CACAAGTGGG AGCGAGCACT CCTCCATCCA GGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623207.1
    CDS388..3243
    Translation

    Target ORF information:

    RefSeq Version XM_005623150.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NLR family pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623150.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGT GAATTCTGGC
    CTCACACCAG GTTGCTGTCC AGCTCTGGCC TCCATGCTCA GCACTAACCA GAAGCTCACA
    CACCTTTACC TACGGGGCAA TGCTCTTGGA GACACAGGGG TGAAGCTACT CTGTGAGGGC
    CTCTTGCACC CCAACTGCAA GCTTCAGATA TTAGAATTGG ATGGCTGTAG CCTCACCTCA
    CACTGTTGCT GGGATCTGTC CACACTTCTG ACCTCCAGCA AGAGCCTCCG GGAGCTAAGC
    CTGGGCAGCA ATGACCTGGG AGATCTAGGG GTCATGCTGC TCTGCGAAGT GCTAAAACAA
    CAGGGCTGCA TCCTACAAAG CCTGAAGTTG TGTGAAATGT ATTTCAATTA TGATACAAAA
    TGTGCATTAG AAACACTTCA AGAAGAAAAG CCTGAGCTGA CCATTGTCTT TGAGCCTTTT
    GGTAGTGACA GAAGCAGGCT GCCAGATGCC AGCGTTTGGG CTCCCTGCCA TCTAGGTGTC
    CACAAGTGGG AGCGAGCACT CCTCCATCCA GGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe10057
    Clone ID Related Accession (Same CDS sequence) XM_005623150.3 , XM_005623150.2
    Accession Version XM_005623150.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726070.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGT GAATTCTGGC
    CTCACACCAG GTTGCTGTCC AGCTCTGGCC TCCATGCTCA GCACTAACCA GAAGCTCACA
    CACCTTTACC TACGGGGCAA TGCTCTTGGA GACACAGGGG TGAAGCTACT CTGTGAGGGC
    CTCTTGCACC CCAACTGCAA GCTTCAGATA TTAGAATTGG ATGGCTGTAG CCTCACCTCA
    CACTGTTGCT GGGATCTGTC CACACTTCTG ACCTCCAGCA AGAGCCTCCG GGAGCTAAGC
    CTGGGCAGCA ATGACCTGGG AGATCTAGGG GTCATGCTGC TCTGCGAAGT GCTAAAACAA
    CAGGGCTGCA TCCTACAAAG CCTGAAGTTG TGTGAAATGT ATTTCAATTA TGATACAAAA
    TGTGCATTAG AAACACTTCA AGAAGAAAAG CCTGAGCTGA CCATTGTCTT TGAGCCTTTT
    GGTAGTGACA GAAGCAGGCT GCCAGATGCC AGCGTTTGGG CTCCCTGCCA TCTAGGTGTC
    CACAAGTGGG AGCGAGCACT CCTCCATCCA GGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623207.1
    CDS319..3174
    Translation

    Target ORF information:

    RefSeq Version XM_005623150.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NLR family, pyrin domain containing 3 (NLRP3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623150.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGT GAATTCTGGC
    CTCACACCAG GTTGCTGTCC AGCTCTGGCC TCCATGCTCA GCACTAACCA GAAGCTCACA
    CACCTTTACC TACGGGGCAA TGCTCTTGGA GACACAGGGG TGAAGCTACT CTGTGAGGGC
    CTCTTGCACC CCAACTGCAA GCTTCAGATA TTAGAATTGG ATGGCTGTAG CCTCACCTCA
    CACTGTTGCT GGGATCTGTC CACACTTCTG ACCTCCAGCA AGAGCCTCCG GGAGCTAAGC
    CTGGGCAGCA ATGACCTGGG AGATCTAGGG GTCATGCTGC TCTGCGAAGT GCTAAAACAA
    CAGGGCTGCA TCCTACAAAG CCTGAAGTTG TGTGAAATGT ATTTCAATTA TGATACAAAA
    TGTGCATTAG AAACACTTCA AGAAGAAAAG CCTGAGCTGA CCATTGTCTT TGAGCCTTTT
    GGTAGTGACA GAAGCAGGCT GCCAGATGCC AGCGTTTGGG CTCCCTGCCA TCTAGGTGTC
    CACAAGTGGG AGCGAGCACT CCTCCATCCA GGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37742
    Clone ID Related Accession (Same CDS sequence) XM_014115621.1
    Accession Version XM_014115621.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726070.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGTTTAT TGGGGTACCT ATCCAGAATC TCTATTTGTA AAAAAAAGAA AGATTACTGC 
    AAGAAGTACA AAAAACACGT GAGGAGCAGA TTCCAGTGTA TTAAAGACAG GAATGCCCGT
    TTGGGTGAGA CTGTGAACCT CAACAAACGC TACACTAGGC TGCGTCTTGT CAAGGAACAC
    CAGAGCCAGC AGGAGAGGGA GCATGAGCTC CTGGCCATTG GCAGGACTTC AGCCAAGACG
    CTGGATAGCC CCATGAGTTC CGTGAATGTG GAATTGCTGT TTGAGCCTGA TGACCAACAC
    TTGGAGCCCG TTCACACAGT GGTATTCCAG GGATCGGCAG GCATTGGGAA AACTATACTG
    GCCAGGAAGA TCATGTTGGA CTGGGCATCA GACAAAATTT ACCAAGACAG GTTTGACTAC
    TTGTTTTATA TCCACTGTCG GGAGGTAAGC CTCGGGACAC GGAGGAGCCT GGGGGACCTG
    ATTGTCAGCT GCTGCCCTGA CCCAAACCCA CCCATATGTA AGATTGTGAG CAAGCCTTCC
    AGGATTCTCT TCCTTATGGA TGGCTTCGAT GAGCTGCAGG GTGCCTTTGA TGAGCATACA
    GAGGCTCTCT GCACAAACTG GCAGAAGGTG GAGCGGGGAG ACATTCTCCT GAGCAGCCTC
    ATCAGGAAGA GGCTGCTTCC TGAGGCCTCT TTACTCATCA CCACAAGACC TGTGGCTCTG
    GAGAAACTGC AGCATTTGCT GGACCGTCCC CGTCATGTGG AGATCCTGGG TTTCTCAGAG
    GCCAAAAGAA AGGAATATTT CTTCAAGTAT TTCTCAGATG AGCAGCAAGC CACAGAAGCT
    TTCAGGCTGA TTCAGGAGAA TGAGATCCTC TTTACCATGT GCTTTATCCC CTTGGTCTGC
    TGGATTGTGT GCACTGGGTT GAAGCAGCAA ATGGACAGTG GCAAGAATCT TGCCCAGACA
    TCCAAGACTA CCACTTCAGT GTATATCTTC TTCCTCTCCA GTTTGTTGCA ATCTCACAGA
    GAGACCCAGA AGCACCAAGT CTCTGCCAGC CTCCGGGGTC TCTGCTCGTT GGCAGCAGAT
    GGAATCTGGA ACCAGAAAAT CCTGTTTGAT GAGTGTGACC TTAGGAATCA CGGCCTTCAG
    AAGGCAGATG TGTCTGCTTT CTTGAGAATG AACCTATTCC AAAAGGAAGT GGACTGTGAG
    AAATTCTACA GCTTCATCCA CATGACTTTC CAGGAGTTCT TTGCTGCCAT GTACTACCTT
    CTGGAAGAGG AGGAACAGGG AAGGATGAGG AACCTGCCAT GGAGTAGTTC CAAGCTTCCC
    AACCGAGATG TGAAGGTTCT TCTTGAAAAC TATGGCAAAT TTGAAAAGGG ATATCTGATT
    TTTGTTGTCC GTTTCCTCTT TGGCCTTGTA AATCAGGAGA GAACCTCCTA CTTGGAGAAA
    AAATTGAGTT GCAAGATCTC TCAACAAATC AGACTGGAGC TGCTCAAATG GATTGAAGAG
    AAAGCCAAGG GCAAGAATCT GCAGATCCAG CCCAGCCAGC TGGAGTTGTT CTATTGTTTG
    TATGAGATGC AAGAGGAGGA TTTTGTGCGA AAGGCCATGG GCCATTTTCC CAAAATTGAG
    ATCAGTCTTT CCACCAGAAT GGACCATGTG GTTTCTTCCT TTTGTATTGA GAACTGTTGC
    AGGGTGGAAT CACTTTCCCT GAGGCTGCTT CATAACTCCC CAAAGGAGGA AGAGGAGGAG
    GAGGAAGAGG AGAATGAGAA GGAAATGCAA CACTATGATG TGGATCACTG TGTCCTCCTA
    GATCCTCATA CCACCTATGC TTATCGGTTG GTGAATTGCC AGGTCACTGT CAGCCTTTGG
    CAGGGTCTCT TCTCAGTCCT GAGCAGAAAC TCACTGAGTC TCACTGAACT GAATCTCAGT
    GACAATGCTC TGGGGGACCA AGGGGTAAAT GTGTTATGTG AAATGCTCCA GCATCCTGGC
    TGTAATATTC GGAAATTGTG GTTGGGGCAG TGCTGCCTTT CCTATCGGTG CTGCTTCAAC
    ATCTCCTCGG TCCTGAGCAA CAACCAGAAG CTGGAGGAAT TGGATCTCAG CCACAACGCC
    CTGGGAGACT TTGGAATTAG ACTTCTTTGT GTGGGGCTGA AGCATCTGTT CTGCAATCTG
    AACAAGCTTT GGTTGGTCAG TTGCTGTCTC ACACCAGCAT GTTGTGAGGA CCTTGCATCC
    GTGCTGAGCA CCAACCAGTC CCTGACCAGA CTCTACCTGG GGGAAAATTC CCTGGAAGAT
    GCAGGAGTTG GAGTTCTATG TGAAAAAGCA AAGCACCCAC AATGTAAACT TCAGAGACTG
    GGGTTGGTGA ATTCTGGCCT CACACCAGGT TGCTGTCCAG CTCTGGCCTC CATGCTCAGC
    ACTAACCAGA AGCTCACACA CCTTTACCTA CGGGGCAATG CTCTTGGAGA CACAGGGGTG
    AAGCTACTCT GTGAGGGCCT CTTGCACCCC AACTGCAAGC TTCAGATATT AGAATTGGAT
    GGCTGTAGCC TCACCTCACA CTGTTGCTGG GATCTGTCCA CACTTCTGAC CTCCAGCAAG
    AGCCTCCGGG AGCTAAGCCT GGGCAGCAAT GACCTGGGAG ATCTAGGGGT CATGCTGCTC
    TGCGAAGTGC TAAAACAACA GGGCTGCATC CTACAAAGCC TGAAGTTGTG TGAAATGTAT
    TTCAATTATG ATACAAAATG TGCATTAGAA ACACTTCAAG AAGAAAAGCC TGAGCTGACC
    ATTGTCTTTG AGCCTTTTGG TAGTGACAGA AGCAGGCTGC CAGATGCCAG CGTTTGGGCT
    CCCTGCCATC TAGGTGTCCA CAAGTGGGAG CGAGCACTCC TCCATCCAGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013971096.1
    CDS192..3065
    Translation

    Target ORF information:

    RefSeq Version XM_014115621.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NLR family, pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115621.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGTTTAT TGGGGTACCT ATCCAGAATC TCTATTTGTA AAAAAAAGAA AGATTACTGC 
    AAGAAGTACA AAAAACACGT GAGGAGCAGA TTCCAGTGTA TTAAAGACAG GAATGCCCGT
    TTGGGTGAGA CTGTGAACCT CAACAAACGC TACACTAGGC TGCGTCTTGT CAAGGAACAC
    CAGAGCCAGC AGGAGAGGGA GCATGAGCTC CTGGCCATTG GCAGGACTTC AGCCAAGACG
    CTGGATAGCC CCATGAGTTC CGTGAATGTG GAATTGCTGT TTGAGCCTGA TGACCAACAC
    TTGGAGCCCG TTCACACAGT GGTATTCCAG GGATCGGCAG GCATTGGGAA AACTATACTG
    GCCAGGAAGA TCATGTTGGA CTGGGCATCA GACAAAATTT ACCAAGACAG GTTTGACTAC
    TTGTTTTATA TCCACTGTCG GGAGGTAAGC CTCGGGACAC GGAGGAGCCT GGGGGACCTG
    ATTGTCAGCT GCTGCCCTGA CCCAAACCCA CCCATATGTA AGATTGTGAG CAAGCCTTCC
    AGGATTCTCT TCCTTATGGA TGGCTTCGAT GAGCTGCAGG GTGCCTTTGA TGAGCATACA
    GAGGCTCTCT GCACAAACTG GCAGAAGGTG GAGCGGGGAG ACATTCTCCT GAGCAGCCTC
    ATCAGGAAGA GGCTGCTTCC TGAGGCCTCT TTACTCATCA CCACAAGACC TGTGGCTCTG
    GAGAAACTGC AGCATTTGCT GGACCGTCCC CGTCATGTGG AGATCCTGGG TTTCTCAGAG
    GCCAAAAGAA AGGAATATTT CTTCAAGTAT TTCTCAGATG AGCAGCAAGC CACAGAAGCT
    TTCAGGCTGA TTCAGGAGAA TGAGATCCTC TTTACCATGT GCTTTATCCC CTTGGTCTGC
    TGGATTGTGT GCACTGGGTT GAAGCAGCAA ATGGACAGTG GCAAGAATCT TGCCCAGACA
    TCCAAGACTA CCACTTCAGT GTATATCTTC TTCCTCTCCA GTTTGTTGCA ATCTCACAGA
    GAGACCCAGA AGCACCAAGT CTCTGCCAGC CTCCGGGGTC TCTGCTCGTT GGCAGCAGAT
    GGAATCTGGA ACCAGAAAAT CCTGTTTGAT GAGTGTGACC TTAGGAATCA CGGCCTTCAG
    AAGGCAGATG TGTCTGCTTT CTTGAGAATG AACCTATTCC AAAAGGAAGT GGACTGTGAG
    AAATTCTACA GCTTCATCCA CATGACTTTC CAGGAGTTCT TTGCTGCCAT GTACTACCTT
    CTGGAAGAGG AGGAACAGGG AAGGATGAGG AACCTGCCAT GGAGTAGTTC CAAGCTTCCC
    AACCGAGATG TGAAGGTTCT TCTTGAAAAC TATGGCAAAT TTGAAAAGGG ATATCTGATT
    TTTGTTGTCC GTTTCCTCTT TGGCCTTGTA AATCAGGAGA GAACCTCCTA CTTGGAGAAA
    AAATTGAGTT GCAAGATCTC TCAACAAATC AGACTGGAGC TGCTCAAATG GATTGAAGAG
    AAAGCCAAGG GCAAGAATCT GCAGATCCAG CCCAGCCAGC TGGAGTTGTT CTATTGTTTG
    TATGAGATGC AAGAGGAGGA TTTTGTGCGA AAGGCCATGG GCCATTTTCC CAAAATTGAG
    ATCAGTCTTT CCACCAGAAT GGACCATGTG GTTTCTTCCT TTTGTATTGA GAACTGTTGC
    AGGGTGGAAT CACTTTCCCT GAGGCTGCTT CATAACTCCC CAAAGGAGGA AGAGGAGGAG
    GAGGAAGAGG AGAATGAGAA GGAAATGCAA CACTATGATG TGGATCACTG TGTCCTCCTA
    GATCCTCATA CCACCTATGC TTATCGGTTG GTGAATTGCC AGGTCACTGT CAGCCTTTGG
    CAGGGTCTCT TCTCAGTCCT GAGCAGAAAC TCACTGAGTC TCACTGAACT GAATCTCAGT
    GACAATGCTC TGGGGGACCA AGGGGTAAAT GTGTTATGTG AAATGCTCCA GCATCCTGGC
    TGTAATATTC GGAAATTGTG GTTGGGGCAG TGCTGCCTTT CCTATCGGTG CTGCTTCAAC
    ATCTCCTCGG TCCTGAGCAA CAACCAGAAG CTGGAGGAAT TGGATCTCAG CCACAACGCC
    CTGGGAGACT TTGGAATTAG ACTTCTTTGT GTGGGGCTGA AGCATCTGTT CTGCAATCTG
    AACAAGCTTT GGTTGGTCAG TTGCTGTCTC ACACCAGCAT GTTGTGAGGA CCTTGCATCC
    GTGCTGAGCA CCAACCAGTC CCTGACCAGA CTCTACCTGG GGGAAAATTC CCTGGAAGAT
    GCAGGAGTTG GAGTTCTATG TGAAAAAGCA AAGCACCCAC AATGTAAACT TCAGAGACTG
    GGGTTGGTGA ATTCTGGCCT CACACCAGGT TGCTGTCCAG CTCTGGCCTC CATGCTCAGC
    ACTAACCAGA AGCTCACACA CCTTTACCTA CGGGGCAATG CTCTTGGAGA CACAGGGGTG
    AAGCTACTCT GTGAGGGCCT CTTGCACCCC AACTGCAAGC TTCAGATATT AGAATTGGAT
    GGCTGTAGCC TCACCTCACA CTGTTGCTGG GATCTGTCCA CACTTCTGAC CTCCAGCAAG
    AGCCTCCGGG AGCTAAGCCT GGGCAGCAAT GACCTGGGAG ATCTAGGGGT CATGCTGCTC
    TGCGAAGTGC TAAAACAACA GGGCTGCATC CTACAAAGCC TGAAGTTGTG TGAAATGTAT
    TTCAATTATG ATACAAAATG TGCATTAGAA ACACTTCAAG AAGAAAAGCC TGAGCTGACC
    ATTGTCTTTG AGCCTTTTGG TAGTGACAGA AGCAGGCTGC CAGATGCCAG CGTTTGGGCT
    CCCTGCCATC TAGGTGTCCA CAAGTGGGAG CGAGCACTCC TCCATCCAGG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe10054
    Clone ID Related Accession (Same CDS sequence) XM_843284.4 , XM_843284.5
    Accession Version XM_843284.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726070.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_843284.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGG GCAGTGCTGC
    CTTTCCTATC GGTGCTGCTT CAACATCTCC TCGGTCCTGA GCAACAACCA GAAGCTGGAG
    GAATTGGATC TCAGCCACAA CGCCCTGGGA GACTTTGGAA TTAGACTTCT TTGTGTGGGG
    CTGAAGCATC TGTTCTGCAA TCTGAACAAG CTTTGGTTGG TCAGTTGCTG TCTCACACCA
    GCATGTTGTG AGGACCTTGC ATCCGTGCTG AGCACCAACC AGTCCCTGAC CAGACTCTAC
    CTGGGGGAAA ATTCCCTGGA AGATGCAGGA GTTGGAGTTC TATGTGAAAA AGCAAAGCAC
    CCACAATGTA AACTTCAGAG ACTGGGGTTG GTGAATTCTG GCCTCACACC AGGTTGCTGT
    CCAGCTCTGG CCTCCATGCT CAGCACTAAC CAGAAGCTCA CACACCTTTA CCTACGGGGC
    AATGCTCTTG GAGACACAGG GGTGAAGCTA CTCTGTGAGG GCCTCTTGCA CCCCAACTGC
    AAGCTTCAGA TATTAGAATT GGATGGCTGT AGCCTCACCT CACACTGTTG CTGGGATCTG
    TCCACACTTC TGACCTCCAG CAAGAGCCTC CGGGAGCTAA GCCTGGGCAG CAATGACCTG
    GGAGATCTAG GGGTCATGCT GCTCTGCGAA GTGCTAAAAC AACAGGGCTG CATCCTACAA
    AGCCTGAAGT TGTGTGAAAT GTATTTCAAT TATGATACAA AATGTGCATT AGAAACACTT
    CAAGAAGAAA AGCCTGAGCT GACCATTGTC TTTGAGCCTT TTGGTAGTGA CAGAAGCAGG
    CTGCCAGATG CCAGCGTTTG GGCTCCCTGC CATCTAGGTG TCCACAAGTG GGAGCGAGCA
    CTCCTCCATC CAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_848377.2
    CDS319..3516
    Translation

    Target ORF information:

    RefSeq Version XM_843284.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NLR family, pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_843284.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGG GCAGTGCTGC
    CTTTCCTATC GGTGCTGCTT CAACATCTCC TCGGTCCTGA GCAACAACCA GAAGCTGGAG
    GAATTGGATC TCAGCCACAA CGCCCTGGGA GACTTTGGAA TTAGACTTCT TTGTGTGGGG
    CTGAAGCATC TGTTCTGCAA TCTGAACAAG CTTTGGTTGG TCAGTTGCTG TCTCACACCA
    GCATGTTGTG AGGACCTTGC ATCCGTGCTG AGCACCAACC AGTCCCTGAC CAGACTCTAC
    CTGGGGGAAA ATTCCCTGGA AGATGCAGGA GTTGGAGTTC TATGTGAAAA AGCAAAGCAC
    CCACAATGTA AACTTCAGAG ACTGGGGTTG GTGAATTCTG GCCTCACACC AGGTTGCTGT
    CCAGCTCTGG CCTCCATGCT CAGCACTAAC CAGAAGCTCA CACACCTTTA CCTACGGGGC
    AATGCTCTTG GAGACACAGG GGTGAAGCTA CTCTGTGAGG GCCTCTTGCA CCCCAACTGC
    AAGCTTCAGA TATTAGAATT GGATGGCTGT AGCCTCACCT CACACTGTTG CTGGGATCTG
    TCCACACTTC TGACCTCCAG CAAGAGCCTC CGGGAGCTAA GCCTGGGCAG CAATGACCTG
    GGAGATCTAG GGGTCATGCT GCTCTGCGAA GTGCTAAAAC AACAGGGCTG CATCCTACAA
    AGCCTGAAGT TGTGTGAAAT GTATTTCAAT TATGATACAA AATGTGCATT AGAAACACTT
    CAAGAAGAAA AGCCTGAGCT GACCATTGTC TTTGAGCCTT TTGGTAGTGA CAGAAGCAGG
    CTGCCAGATG CCAGCGTTTG GGCTCCCTGC CATCTAGGTG TCCACAAGTG GGAGCGAGCA
    CTCCTCCATC CAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe10054
    Clone ID Related Accession (Same CDS sequence) XM_843284.4 , XM_843284.5
    Accession Version XM_843284.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_843284.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGG GCAGTGCTGC
    CTTTCCTATC GGTGCTGCTT CAACATCTCC TCGGTCCTGA GCAACAACCA GAAGCTGGAG
    GAATTGGATC TCAGCCACAA CGCCCTGGGA GACTTTGGAA TTAGACTTCT TTGTGTGGGG
    CTGAAGCATC TGTTCTGCAA TCTGAACAAG CTTTGGTTGG TCAGTTGCTG TCTCACACCA
    GCATGTTGTG AGGACCTTGC ATCCGTGCTG AGCACCAACC AGTCCCTGAC CAGACTCTAC
    CTGGGGGAAA ATTCCCTGGA AGATGCAGGA GTTGGAGTTC TATGTGAAAA AGCAAAGCAC
    CCACAATGTA AACTTCAGAG ACTGGGGTTG GTGAATTCTG GCCTCACACC AGGTTGCTGT
    CCAGCTCTGG CCTCCATGCT CAGCACTAAC CAGAAGCTCA CACACCTTTA CCTACGGGGC
    AATGCTCTTG GAGACACAGG GGTGAAGCTA CTCTGTGAGG GCCTCTTGCA CCCCAACTGC
    AAGCTTCAGA TATTAGAATT GGATGGCTGT AGCCTCACCT CACACTGTTG CTGGGATCTG
    TCCACACTTC TGACCTCCAG CAAGAGCCTC CGGGAGCTAA GCCTGGGCAG CAATGACCTG
    GGAGATCTAG GGGTCATGCT GCTCTGCGAA GTGCTAAAAC AACAGGGCTG CATCCTACAA
    AGCCTGAAGT TGTGTGAAAT GTATTTCAAT TATGATACAA AATGTGCATT AGAAACACTT
    CAAGAAGAAA AGCCTGAGCT GACCATTGTC TTTGAGCCTT TTGGTAGTGA CAGAAGCAGG
    CTGCCAGATG CCAGCGTTTG GGCTCCCTGC CATCTAGGTG TCCACAAGTG GGAGCGAGCA
    CTCCTCCATC CAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_848377.2
    CDS387..3584
    Translation

    Target ORF information:

    RefSeq Version XM_843284.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NLR family pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_843284.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGG GCAGTGCTGC
    CTTTCCTATC GGTGCTGCTT CAACATCTCC TCGGTCCTGA GCAACAACCA GAAGCTGGAG
    GAATTGGATC TCAGCCACAA CGCCCTGGGA GACTTTGGAA TTAGACTTCT TTGTGTGGGG
    CTGAAGCATC TGTTCTGCAA TCTGAACAAG CTTTGGTTGG TCAGTTGCTG TCTCACACCA
    GCATGTTGTG AGGACCTTGC ATCCGTGCTG AGCACCAACC AGTCCCTGAC CAGACTCTAC
    CTGGGGGAAA ATTCCCTGGA AGATGCAGGA GTTGGAGTTC TATGTGAAAA AGCAAAGCAC
    CCACAATGTA AACTTCAGAG ACTGGGGTTG GTGAATTCTG GCCTCACACC AGGTTGCTGT
    CCAGCTCTGG CCTCCATGCT CAGCACTAAC CAGAAGCTCA CACACCTTTA CCTACGGGGC
    AATGCTCTTG GAGACACAGG GGTGAAGCTA CTCTGTGAGG GCCTCTTGCA CCCCAACTGC
    AAGCTTCAGA TATTAGAATT GGATGGCTGT AGCCTCACCT CACACTGTTG CTGGGATCTG
    TCCACACTTC TGACCTCCAG CAAGAGCCTC CGGGAGCTAA GCCTGGGCAG CAATGACCTG
    GGAGATCTAG GGGTCATGCT GCTCTGCGAA GTGCTAAAAC AACAGGGCTG CATCCTACAA
    AGCCTGAAGT TGTGTGAAAT GTATTTCAAT TATGATACAA AATGTGCATT AGAAACACTT
    CAAGAAGAAA AGCCTGAGCT GACCATTGTC TTTGAGCCTT TTGGTAGTGA CAGAAGCAGG
    CTGCCAGATG CCAGCGTTTG GGCTCCCTGC CATCTAGGTG TCCACAAGTG GGAGCGAGCA
    CTCCTCCATC CAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe10055
    Clone ID Related Accession (Same CDS sequence) XM_005623148.3 , XM_005623148.2
    Accession Version XM_005623148.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GATAATTCAC ATCTGGAATG CCGAGAAGAA AGCCTTGAAG AGGAGTGGAT GGGTTTATTG
    GGGTACCTAT CCAGAATCTC TATTTGTAAA AAAAAGAAAG ATTACTGCAA GAAGTACAAA
    AAACACGTGA GGAGCAGATT CCAGTGTATT AAAGACAGGA ATGCCCGTTT GGGTGAGACT
    GTGAACCTCA ACAAACGCTA CACTAGGCTG CGTCTTGTCA AGGAACACCA GAGCCAGCAG
    GAGAGGGAGC ATGAGCTCCT GGCCATTGGC AGGACTTCAG CCAAGACGCT GGATAGCCCC
    ATGAGTTCCG TGAATGTGGA ATTGCTGTTT GAGCCTGATG ACCAACACTT GGAGCCCGTT
    CACACAGTGG TATTCCAGGG ATCGGCAGGC ATTGGGAAAA CTATACTGGC CAGGAAGATC
    ATGTTGGACT GGGCATCAGA CAAAATTTAC CAAGACAGGT TTGACTACTT GTTTTATATC
    CACTGTCGGG AGGTAAGCCT CGGGACACGG AGGAGCCTGG GGGACCTGAT TGTCAGCTGC
    TGCCCTGACC CAAACCCACC CATATGTAAG ATTGTGAGCA AGCCTTCCAG GATTCTCTTC
    CTTATGGATG GCTTCGATGA GCTGCAGGGT GCCTTTGATG AGCATACAGA GGCTCTCTGC
    ACAAACTGGC AGAAGGTGGA GCGGGGAGAC ATTCTCCTGA GCAGCCTCAT CAGGAAGAGG
    CTGCTTCCTG AGGCCTCTTT ACTCATCACC ACAAGACCTG TGGCTCTGGA GAAACTGCAG
    CATTTGCTGG ACCGTCCCCG TCATGTGGAG ATCCTGGGTT TCTCAGAGGC CAAAAGAAAG
    GAATATTTCT TCAAGTATTT CTCAGATGAG CAGCAAGCCA CAGAAGCTTT CAGGCTGATT
    CAGGAGAATG AGATCCTCTT TACCATGTGC TTTATCCCCT TGGTCTGCTG GATTGTGTGC
    ACTGGGTTGA AGCAGCAAAT GGACAGTGGC AAGAATCTTG CCCAGACATC CAAGACTACC
    ACTTCAGTGT ATATCTTCTT CCTCTCCAGT TTGTTGCAAT CTCACAGAGA GACCCAGAAG
    CACCAAGTCT CTGCCAGCCT CCGGGGTCTC TGCTCGTTGG CAGCAGATGG AATCTGGAAC
    CAGAAAATCC TGTTTGATGA GTGTGACCTT AGGAATCACG GCCTTCAGAA GGCAGATGTG
    TCTGCTTTCT TGAGAATGAA CCTATTCCAA AAGGAAGTGG ACTGTGAGAA ATTCTACAGC
    TTCATCCACA TGACTTTCCA GGAGTTCTTT GCTGCCATGT ACTACCTTCT GGAAGAGGAG
    GAACAGGGAA GGATGAGGAA CCTGCCATGG AGTAGTTCCA AGCTTCCCAA CCGAGATGTG
    AAGGTTCTTC TTGAAAACTA TGGCAAATTT GAAAAGGGAT ATCTGATTTT TGTTGTCCGT
    TTCCTCTTTG GCCTTGTAAA TCAGGAGAGA ACCTCCTACT TGGAGAAAAA ATTGAGTTGC
    AAGATCTCTC AACAAATCAG ACTGGAGCTG CTCAAATGGA TTGAAGAGAA AGCCAAGGGC
    AAGAATCTGC AGATCCAGCC CAGCCAGCTG GAGTTGTTCT ATTGTTTGTA TGAGATGCAA
    GAGGAGGATT TTGTGCGAAA GGCCATGGGC CATTTTCCCA AAATTGAGAT CAGTCTTTCC
    ACCAGAATGG ACCATGTGGT TTCTTCCTTT TGTATTGAGA ACTGTTGCAG GGTGGAATCA
    CTTTCCCTGA GGCTGCTTCA TAACTCCCCA AAGGAGGAAG AGGAGGAGGA GGAAGAGGAG
    AATGAGAAGG AAATGCAACA CTATGATGTG GATCACTGTG TCCTCCTAGA TCCTCATACC
    ACCTATGCTT ATCGGTTGGT GAATTGCCAG GTCACTGTCA GCCTTTGGCA GGGTCTCTTC
    TCAGTCCTGA GCAGAAACTC ACTGAGTCTC ACTGAACTGA ATCTCAGTGA CAATGCTCTG
    GGGGACCAAG GGGTAAATGT GTTATGTGAA ATGCTCCAGC ATCCTGGCTG TAATATTCGG
    AAATTGTGGT TGGGGCAGTG CTGCCTTTCC TATCGGTGCT GCTTCAACAT CTCCTCGGTC
    CTGAGCAACA ACCAGAAGCT GGAGGAATTG GATCTCAGCC ACAACGCCCT GGGAGACTTT
    GGAATTAGAC TTCTTTGTGT GGGGCTGAAG CATCTGTTCT GCAATCTGAA CAAGCTTTGG
    TTGGTCAGTT GCTGTCTCAC ACCAGCATGT TGTGAGGACC TTGCATCCGT GCTGAGCACC
    AACCAGTCCC TGACCAGACT CTACCTGGGG GAAAATTCCC TGGAAGATGC AGGAGTTGGA
    GTTCTATGTG AAAAAGCAAA GCACCCACAA TGTAAACTTC AGAGACTGGG GTTGGTGAAT
    TCTGGCCTCA CACCAGGTTG CTGTCCAGCT CTGGCCTCCA TGCTCAGCAC TAACCAGAAG
    CTCACACACC TTTACCTACG GGGCAATGCT CTTGGAGACA CAGGGGTGAA GCTACTCTGT
    GAGGGCCTCT TGCACCCCAA CTGCAAGCTT CAGATATTAG AATTGGATGG CTGTAGCCTC
    ACCTCACACT GTTGCTGGGA TCTGTCCACA CTTCTGACCT CCAGCAAGAG CCTCCGGGAG
    CTAAGCCTGG GCAGCAATGA CCTGGGAGAT CTAGGGGTCA TGCTGCTCTG CGAAGTGCTA
    AAACAACAGG GCTGCATCCT ACAAAGCCTG AAGTTGTGTG AAATGTATTT CAATTATGAT
    ACAAAATGTG CATTAGAAAC ACTTCAAGAA GAAAAGCCTG AGCTGACCAT TGTCTTTGAG
    CCTTTTGGTA GTGACAGAAG CAGGCTGCCA GATGCCAGCG TTTGGGCTCC CTGCCATCTA
    GGTGTCCACA AGTGGGAGCG AGCACTCCTC CATCCAGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623205.1
    CDS387..3488
    Translation

    Target ORF information:

    RefSeq Version XM_005623148.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NLR family pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623148.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GATAATTCAC ATCTGGAATG CCGAGAAGAA AGCCTTGAAG AGGAGTGGAT GGGTTTATTG
    GGGTACCTAT CCAGAATCTC TATTTGTAAA AAAAAGAAAG ATTACTGCAA GAAGTACAAA
    AAACACGTGA GGAGCAGATT CCAGTGTATT AAAGACAGGA ATGCCCGTTT GGGTGAGACT
    GTGAACCTCA ACAAACGCTA CACTAGGCTG CGTCTTGTCA AGGAACACCA GAGCCAGCAG
    GAGAGGGAGC ATGAGCTCCT GGCCATTGGC AGGACTTCAG CCAAGACGCT GGATAGCCCC
    ATGAGTTCCG TGAATGTGGA ATTGCTGTTT GAGCCTGATG ACCAACACTT GGAGCCCGTT
    CACACAGTGG TATTCCAGGG ATCGGCAGGC ATTGGGAAAA CTATACTGGC CAGGAAGATC
    ATGTTGGACT GGGCATCAGA CAAAATTTAC CAAGACAGGT TTGACTACTT GTTTTATATC
    CACTGTCGGG AGGTAAGCCT CGGGACACGG AGGAGCCTGG GGGACCTGAT TGTCAGCTGC
    TGCCCTGACC CAAACCCACC CATATGTAAG ATTGTGAGCA AGCCTTCCAG GATTCTCTTC
    CTTATGGATG GCTTCGATGA GCTGCAGGGT GCCTTTGATG AGCATACAGA GGCTCTCTGC
    ACAAACTGGC AGAAGGTGGA GCGGGGAGAC ATTCTCCTGA GCAGCCTCAT CAGGAAGAGG
    CTGCTTCCTG AGGCCTCTTT ACTCATCACC ACAAGACCTG TGGCTCTGGA GAAACTGCAG
    CATTTGCTGG ACCGTCCCCG TCATGTGGAG ATCCTGGGTT TCTCAGAGGC CAAAAGAAAG
    GAATATTTCT TCAAGTATTT CTCAGATGAG CAGCAAGCCA CAGAAGCTTT CAGGCTGATT
    CAGGAGAATG AGATCCTCTT TACCATGTGC TTTATCCCCT TGGTCTGCTG GATTGTGTGC
    ACTGGGTTGA AGCAGCAAAT GGACAGTGGC AAGAATCTTG CCCAGACATC CAAGACTACC
    ACTTCAGTGT ATATCTTCTT CCTCTCCAGT TTGTTGCAAT CTCACAGAGA GACCCAGAAG
    CACCAAGTCT CTGCCAGCCT CCGGGGTCTC TGCTCGTTGG CAGCAGATGG AATCTGGAAC
    CAGAAAATCC TGTTTGATGA GTGTGACCTT AGGAATCACG GCCTTCAGAA GGCAGATGTG
    TCTGCTTTCT TGAGAATGAA CCTATTCCAA AAGGAAGTGG ACTGTGAGAA ATTCTACAGC
    TTCATCCACA TGACTTTCCA GGAGTTCTTT GCTGCCATGT ACTACCTTCT GGAAGAGGAG
    GAACAGGGAA GGATGAGGAA CCTGCCATGG AGTAGTTCCA AGCTTCCCAA CCGAGATGTG
    AAGGTTCTTC TTGAAAACTA TGGCAAATTT GAAAAGGGAT ATCTGATTTT TGTTGTCCGT
    TTCCTCTTTG GCCTTGTAAA TCAGGAGAGA ACCTCCTACT TGGAGAAAAA ATTGAGTTGC
    AAGATCTCTC AACAAATCAG ACTGGAGCTG CTCAAATGGA TTGAAGAGAA AGCCAAGGGC
    AAGAATCTGC AGATCCAGCC CAGCCAGCTG GAGTTGTTCT ATTGTTTGTA TGAGATGCAA
    GAGGAGGATT TTGTGCGAAA GGCCATGGGC CATTTTCCCA AAATTGAGAT CAGTCTTTCC
    ACCAGAATGG ACCATGTGGT TTCTTCCTTT TGTATTGAGA ACTGTTGCAG GGTGGAATCA
    CTTTCCCTGA GGCTGCTTCA TAACTCCCCA AAGGAGGAAG AGGAGGAGGA GGAAGAGGAG
    AATGAGAAGG AAATGCAACA CTATGATGTG GATCACTGTG TCCTCCTAGA TCCTCATACC
    ACCTATGCTT ATCGGTTGGT GAATTGCCAG GTCACTGTCA GCCTTTGGCA GGGTCTCTTC
    TCAGTCCTGA GCAGAAACTC ACTGAGTCTC ACTGAACTGA ATCTCAGTGA CAATGCTCTG
    GGGGACCAAG GGGTAAATGT GTTATGTGAA ATGCTCCAGC ATCCTGGCTG TAATATTCGG
    AAATTGTGGT TGGGGCAGTG CTGCCTTTCC TATCGGTGCT GCTTCAACAT CTCCTCGGTC
    CTGAGCAACA ACCAGAAGCT GGAGGAATTG GATCTCAGCC ACAACGCCCT GGGAGACTTT
    GGAATTAGAC TTCTTTGTGT GGGGCTGAAG CATCTGTTCT GCAATCTGAA CAAGCTTTGG
    TTGGTCAGTT GCTGTCTCAC ACCAGCATGT TGTGAGGACC TTGCATCCGT GCTGAGCACC
    AACCAGTCCC TGACCAGACT CTACCTGGGG GAAAATTCCC TGGAAGATGC AGGAGTTGGA
    GTTCTATGTG AAAAAGCAAA GCACCCACAA TGTAAACTTC AGAGACTGGG GTTGGTGAAT
    TCTGGCCTCA CACCAGGTTG CTGTCCAGCT CTGGCCTCCA TGCTCAGCAC TAACCAGAAG
    CTCACACACC TTTACCTACG GGGCAATGCT CTTGGAGACA CAGGGGTGAA GCTACTCTGT
    GAGGGCCTCT TGCACCCCAA CTGCAAGCTT CAGATATTAG AATTGGATGG CTGTAGCCTC
    ACCTCACACT GTTGCTGGGA TCTGTCCACA CTTCTGACCT CCAGCAAGAG CCTCCGGGAG
    CTAAGCCTGG GCAGCAATGA CCTGGGAGAT CTAGGGGTCA TGCTGCTCTG CGAAGTGCTA
    AAACAACAGG GCTGCATCCT ACAAAGCCTG AAGTTGTGTG AAATGTATTT CAATTATGAT
    ACAAAATGTG CATTAGAAAC ACTTCAAGAA GAAAAGCCTG AGCTGACCAT TGTCTTTGAG
    CCTTTTGGTA GTGACAGAAG CAGGCTGCCA GATGCCAGCG TTTGGGCTCC CTGCCATCTA
    GGTGTCCACA AGTGGGAGCG AGCACTCCTC CATCCAGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe10055
    Clone ID Related Accession (Same CDS sequence) XM_005623148.3 , XM_005623148.2
    Accession Version XM_005623148.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726070.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GATAATTCAC ATCTGGAATG CCGAGAAGAA AGCCTTGAAG AGGAGTGGAT GGGTTTATTG
    GGGTACCTAT CCAGAATCTC TATTTGTAAA AAAAAGAAAG ATTACTGCAA GAAGTACAAA
    AAACACGTGA GGAGCAGATT CCAGTGTATT AAAGACAGGA ATGCCCGTTT GGGTGAGACT
    GTGAACCTCA ACAAACGCTA CACTAGGCTG CGTCTTGTCA AGGAACACCA GAGCCAGCAG
    GAGAGGGAGC ATGAGCTCCT GGCCATTGGC AGGACTTCAG CCAAGACGCT GGATAGCCCC
    ATGAGTTCCG TGAATGTGGA ATTGCTGTTT GAGCCTGATG ACCAACACTT GGAGCCCGTT
    CACACAGTGG TATTCCAGGG ATCGGCAGGC ATTGGGAAAA CTATACTGGC CAGGAAGATC
    ATGTTGGACT GGGCATCAGA CAAAATTTAC CAAGACAGGT TTGACTACTT GTTTTATATC
    CACTGTCGGG AGGTAAGCCT CGGGACACGG AGGAGCCTGG GGGACCTGAT TGTCAGCTGC
    TGCCCTGACC CAAACCCACC CATATGTAAG ATTGTGAGCA AGCCTTCCAG GATTCTCTTC
    CTTATGGATG GCTTCGATGA GCTGCAGGGT GCCTTTGATG AGCATACAGA GGCTCTCTGC
    ACAAACTGGC AGAAGGTGGA GCGGGGAGAC ATTCTCCTGA GCAGCCTCAT CAGGAAGAGG
    CTGCTTCCTG AGGCCTCTTT ACTCATCACC ACAAGACCTG TGGCTCTGGA GAAACTGCAG
    CATTTGCTGG ACCGTCCCCG TCATGTGGAG ATCCTGGGTT TCTCAGAGGC CAAAAGAAAG
    GAATATTTCT TCAAGTATTT CTCAGATGAG CAGCAAGCCA CAGAAGCTTT CAGGCTGATT
    CAGGAGAATG AGATCCTCTT TACCATGTGC TTTATCCCCT TGGTCTGCTG GATTGTGTGC
    ACTGGGTTGA AGCAGCAAAT GGACAGTGGC AAGAATCTTG CCCAGACATC CAAGACTACC
    ACTTCAGTGT ATATCTTCTT CCTCTCCAGT TTGTTGCAAT CTCACAGAGA GACCCAGAAG
    CACCAAGTCT CTGCCAGCCT CCGGGGTCTC TGCTCGTTGG CAGCAGATGG AATCTGGAAC
    CAGAAAATCC TGTTTGATGA GTGTGACCTT AGGAATCACG GCCTTCAGAA GGCAGATGTG
    TCTGCTTTCT TGAGAATGAA CCTATTCCAA AAGGAAGTGG ACTGTGAGAA ATTCTACAGC
    TTCATCCACA TGACTTTCCA GGAGTTCTTT GCTGCCATGT ACTACCTTCT GGAAGAGGAG
    GAACAGGGAA GGATGAGGAA CCTGCCATGG AGTAGTTCCA AGCTTCCCAA CCGAGATGTG
    AAGGTTCTTC TTGAAAACTA TGGCAAATTT GAAAAGGGAT ATCTGATTTT TGTTGTCCGT
    TTCCTCTTTG GCCTTGTAAA TCAGGAGAGA ACCTCCTACT TGGAGAAAAA ATTGAGTTGC
    AAGATCTCTC AACAAATCAG ACTGGAGCTG CTCAAATGGA TTGAAGAGAA AGCCAAGGGC
    AAGAATCTGC AGATCCAGCC CAGCCAGCTG GAGTTGTTCT ATTGTTTGTA TGAGATGCAA
    GAGGAGGATT TTGTGCGAAA GGCCATGGGC CATTTTCCCA AAATTGAGAT CAGTCTTTCC
    ACCAGAATGG ACCATGTGGT TTCTTCCTTT TGTATTGAGA ACTGTTGCAG GGTGGAATCA
    CTTTCCCTGA GGCTGCTTCA TAACTCCCCA AAGGAGGAAG AGGAGGAGGA GGAAGAGGAG
    AATGAGAAGG AAATGCAACA CTATGATGTG GATCACTGTG TCCTCCTAGA TCCTCATACC
    ACCTATGCTT ATCGGTTGGT GAATTGCCAG GTCACTGTCA GCCTTTGGCA GGGTCTCTTC
    TCAGTCCTGA GCAGAAACTC ACTGAGTCTC ACTGAACTGA ATCTCAGTGA CAATGCTCTG
    GGGGACCAAG GGGTAAATGT GTTATGTGAA ATGCTCCAGC ATCCTGGCTG TAATATTCGG
    AAATTGTGGT TGGGGCAGTG CTGCCTTTCC TATCGGTGCT GCTTCAACAT CTCCTCGGTC
    CTGAGCAACA ACCAGAAGCT GGAGGAATTG GATCTCAGCC ACAACGCCCT GGGAGACTTT
    GGAATTAGAC TTCTTTGTGT GGGGCTGAAG CATCTGTTCT GCAATCTGAA CAAGCTTTGG
    TTGGTCAGTT GCTGTCTCAC ACCAGCATGT TGTGAGGACC TTGCATCCGT GCTGAGCACC
    AACCAGTCCC TGACCAGACT CTACCTGGGG GAAAATTCCC TGGAAGATGC AGGAGTTGGA
    GTTCTATGTG AAAAAGCAAA GCACCCACAA TGTAAACTTC AGAGACTGGG GTTGGTGAAT
    TCTGGCCTCA CACCAGGTTG CTGTCCAGCT CTGGCCTCCA TGCTCAGCAC TAACCAGAAG
    CTCACACACC TTTACCTACG GGGCAATGCT CTTGGAGACA CAGGGGTGAA GCTACTCTGT
    GAGGGCCTCT TGCACCCCAA CTGCAAGCTT CAGATATTAG AATTGGATGG CTGTAGCCTC
    ACCTCACACT GTTGCTGGGA TCTGTCCACA CTTCTGACCT CCAGCAAGAG CCTCCGGGAG
    CTAAGCCTGG GCAGCAATGA CCTGGGAGAT CTAGGGGTCA TGCTGCTCTG CGAAGTGCTA
    AAACAACAGG GCTGCATCCT ACAAAGCCTG AAGTTGTGTG AAATGTATTT CAATTATGAT
    ACAAAATGTG CATTAGAAAC ACTTCAAGAA GAAAAGCCTG AGCTGACCAT TGTCTTTGAG
    CCTTTTGGTA GTGACAGAAG CAGGCTGCCA GATGCCAGCG TTTGGGCTCC CTGCCATCTA
    GGTGTCCACA AGTGGGAGCG AGCACTCCTC CATCCAGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623205.1
    CDS319..3420
    Translation

    Target ORF information:

    RefSeq Version XM_005623148.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NLR family, pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623148.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GATAATTCAC ATCTGGAATG CCGAGAAGAA AGCCTTGAAG AGGAGTGGAT GGGTTTATTG
    GGGTACCTAT CCAGAATCTC TATTTGTAAA AAAAAGAAAG ATTACTGCAA GAAGTACAAA
    AAACACGTGA GGAGCAGATT CCAGTGTATT AAAGACAGGA ATGCCCGTTT GGGTGAGACT
    GTGAACCTCA ACAAACGCTA CACTAGGCTG CGTCTTGTCA AGGAACACCA GAGCCAGCAG
    GAGAGGGAGC ATGAGCTCCT GGCCATTGGC AGGACTTCAG CCAAGACGCT GGATAGCCCC
    ATGAGTTCCG TGAATGTGGA ATTGCTGTTT GAGCCTGATG ACCAACACTT GGAGCCCGTT
    CACACAGTGG TATTCCAGGG ATCGGCAGGC ATTGGGAAAA CTATACTGGC CAGGAAGATC
    ATGTTGGACT GGGCATCAGA CAAAATTTAC CAAGACAGGT TTGACTACTT GTTTTATATC
    CACTGTCGGG AGGTAAGCCT CGGGACACGG AGGAGCCTGG GGGACCTGAT TGTCAGCTGC
    TGCCCTGACC CAAACCCACC CATATGTAAG ATTGTGAGCA AGCCTTCCAG GATTCTCTTC
    CTTATGGATG GCTTCGATGA GCTGCAGGGT GCCTTTGATG AGCATACAGA GGCTCTCTGC
    ACAAACTGGC AGAAGGTGGA GCGGGGAGAC ATTCTCCTGA GCAGCCTCAT CAGGAAGAGG
    CTGCTTCCTG AGGCCTCTTT ACTCATCACC ACAAGACCTG TGGCTCTGGA GAAACTGCAG
    CATTTGCTGG ACCGTCCCCG TCATGTGGAG ATCCTGGGTT TCTCAGAGGC CAAAAGAAAG
    GAATATTTCT TCAAGTATTT CTCAGATGAG CAGCAAGCCA CAGAAGCTTT CAGGCTGATT
    CAGGAGAATG AGATCCTCTT TACCATGTGC TTTATCCCCT TGGTCTGCTG GATTGTGTGC
    ACTGGGTTGA AGCAGCAAAT GGACAGTGGC AAGAATCTTG CCCAGACATC CAAGACTACC
    ACTTCAGTGT ATATCTTCTT CCTCTCCAGT TTGTTGCAAT CTCACAGAGA GACCCAGAAG
    CACCAAGTCT CTGCCAGCCT CCGGGGTCTC TGCTCGTTGG CAGCAGATGG AATCTGGAAC
    CAGAAAATCC TGTTTGATGA GTGTGACCTT AGGAATCACG GCCTTCAGAA GGCAGATGTG
    TCTGCTTTCT TGAGAATGAA CCTATTCCAA AAGGAAGTGG ACTGTGAGAA ATTCTACAGC
    TTCATCCACA TGACTTTCCA GGAGTTCTTT GCTGCCATGT ACTACCTTCT GGAAGAGGAG
    GAACAGGGAA GGATGAGGAA CCTGCCATGG AGTAGTTCCA AGCTTCCCAA CCGAGATGTG
    AAGGTTCTTC TTGAAAACTA TGGCAAATTT GAAAAGGGAT ATCTGATTTT TGTTGTCCGT
    TTCCTCTTTG GCCTTGTAAA TCAGGAGAGA ACCTCCTACT TGGAGAAAAA ATTGAGTTGC
    AAGATCTCTC AACAAATCAG ACTGGAGCTG CTCAAATGGA TTGAAGAGAA AGCCAAGGGC
    AAGAATCTGC AGATCCAGCC CAGCCAGCTG GAGTTGTTCT ATTGTTTGTA TGAGATGCAA
    GAGGAGGATT TTGTGCGAAA GGCCATGGGC CATTTTCCCA AAATTGAGAT CAGTCTTTCC
    ACCAGAATGG ACCATGTGGT TTCTTCCTTT TGTATTGAGA ACTGTTGCAG GGTGGAATCA
    CTTTCCCTGA GGCTGCTTCA TAACTCCCCA AAGGAGGAAG AGGAGGAGGA GGAAGAGGAG
    AATGAGAAGG AAATGCAACA CTATGATGTG GATCACTGTG TCCTCCTAGA TCCTCATACC
    ACCTATGCTT ATCGGTTGGT GAATTGCCAG GTCACTGTCA GCCTTTGGCA GGGTCTCTTC
    TCAGTCCTGA GCAGAAACTC ACTGAGTCTC ACTGAACTGA ATCTCAGTGA CAATGCTCTG
    GGGGACCAAG GGGTAAATGT GTTATGTGAA ATGCTCCAGC ATCCTGGCTG TAATATTCGG
    AAATTGTGGT TGGGGCAGTG CTGCCTTTCC TATCGGTGCT GCTTCAACAT CTCCTCGGTC
    CTGAGCAACA ACCAGAAGCT GGAGGAATTG GATCTCAGCC ACAACGCCCT GGGAGACTTT
    GGAATTAGAC TTCTTTGTGT GGGGCTGAAG CATCTGTTCT GCAATCTGAA CAAGCTTTGG
    TTGGTCAGTT GCTGTCTCAC ACCAGCATGT TGTGAGGACC TTGCATCCGT GCTGAGCACC
    AACCAGTCCC TGACCAGACT CTACCTGGGG GAAAATTCCC TGGAAGATGC AGGAGTTGGA
    GTTCTATGTG AAAAAGCAAA GCACCCACAA TGTAAACTTC AGAGACTGGG GTTGGTGAAT
    TCTGGCCTCA CACCAGGTTG CTGTCCAGCT CTGGCCTCCA TGCTCAGCAC TAACCAGAAG
    CTCACACACC TTTACCTACG GGGCAATGCT CTTGGAGACA CAGGGGTGAA GCTACTCTGT
    GAGGGCCTCT TGCACCCCAA CTGCAAGCTT CAGATATTAG AATTGGATGG CTGTAGCCTC
    ACCTCACACT GTTGCTGGGA TCTGTCCACA CTTCTGACCT CCAGCAAGAG CCTCCGGGAG
    CTAAGCCTGG GCAGCAATGA CCTGGGAGAT CTAGGGGTCA TGCTGCTCTG CGAAGTGCTA
    AAACAACAGG GCTGCATCCT ACAAAGCCTG AAGTTGTGTG AAATGTATTT CAATTATGAT
    ACAAAATGTG CATTAGAAAC ACTTCAAGAA GAAAAGCCTG AGCTGACCAT TGTCTTTGAG
    CCTTTTGGTA GTGACAGAAG CAGGCTGCCA GATGCCAGCG TTTGGGCTCC CTGCCATCTA
    GGTGTCCACA AGTGGGAGCG AGCACTCCTC CATCCAGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe10056
    Clone ID Related Accession (Same CDS sequence) XM_005623149.3 , XM_005623149.2
    Accession Version XM_005623149.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3027bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623149.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGG GCAGTGCTGC
    CTTTCCTATC GGTGCTGCTT CAACATCTCC TCGGTCCTGA GCAACAACCA GAAGCTGGAG
    GAATTGGATC TCAGCCACAA CGCCCTGGGA GACTTTGGAA TTAGACTTCT TTGTGTGGGG
    CTGAAGCATC TGTTCTGCAA TCTGAACAAG CTTTGGTTGG TGAATTCTGG CCTCACACCA
    GGTTGCTGTC CAGCTCTGGC CTCCATGCTC AGCACTAACC AGAAGCTCAC ACACCTTTAC
    CTACGGGGCA ATGCTCTTGG AGACACAGGG GTGAAGCTAC TCTGTGAGGG CCTCTTGCAC
    CCCAACTGCA AGCTTCAGAT ATTAGAATTG GATGGCTGTA GCCTCACCTC ACACTGTTGC
    TGGGATCTGT CCACACTTCT GACCTCCAGC AAGAGCCTCC GGGAGCTAAG CCTGGGCAGC
    AATGACCTGG GAGATCTAGG GGTCATGCTG CTCTGCGAAG TGCTAAAACA ACAGGGCTGC
    ATCCTACAAA GCCTGAAGTT GTGTGAAATG TATTTCAATT ATGATACAAA ATGTGCATTA
    GAAACACTTC AAGAAGAAAA GCCTGAGCTG ACCATTGTCT TTGAGCCTTT TGGTAGTGAC
    AGAAGCAGGC TGCCAGATGC CAGCGTTTGG GCTCCCTGCC ATCTAGGTGT CCACAAGTGG
    GAGCGAGCAC TCCTCCATCC AGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623206.1
    CDS388..3414
    Translation

    Target ORF information:

    RefSeq Version XM_005623149.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NLR family pyrin domain containing 3 (NLRP3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623149.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGG GCAGTGCTGC
    CTTTCCTATC GGTGCTGCTT CAACATCTCC TCGGTCCTGA GCAACAACCA GAAGCTGGAG
    GAATTGGATC TCAGCCACAA CGCCCTGGGA GACTTTGGAA TTAGACTTCT TTGTGTGGGG
    CTGAAGCATC TGTTCTGCAA TCTGAACAAG CTTTGGTTGG TGAATTCTGG CCTCACACCA
    GGTTGCTGTC CAGCTCTGGC CTCCATGCTC AGCACTAACC AGAAGCTCAC ACACCTTTAC
    CTACGGGGCA ATGCTCTTGG AGACACAGGG GTGAAGCTAC TCTGTGAGGG CCTCTTGCAC
    CCCAACTGCA AGCTTCAGAT ATTAGAATTG GATGGCTGTA GCCTCACCTC ACACTGTTGC
    TGGGATCTGT CCACACTTCT GACCTCCAGC AAGAGCCTCC GGGAGCTAAG CCTGGGCAGC
    AATGACCTGG GAGATCTAGG GGTCATGCTG CTCTGCGAAG TGCTAAAACA ACAGGGCTGC
    ATCCTACAAA GCCTGAAGTT GTGTGAAATG TATTTCAATT ATGATACAAA ATGTGCATTA
    GAAACACTTC AAGAAGAAAA GCCTGAGCTG ACCATTGTCT TTGAGCCTTT TGGTAGTGAC
    AGAAGCAGGC TGCCAGATGC CAGCGTTTGG GCTCCCTGCC ATCTAGGTGT CCACAAGTGG
    GAGCGAGCAC TCCTCCATCC AGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe10056
    Clone ID Related Accession (Same CDS sequence) XM_005623149.3 , XM_005623149.2
    Accession Version XM_005623149.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3027bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726070.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGG GCAGTGCTGC
    CTTTCCTATC GGTGCTGCTT CAACATCTCC TCGGTCCTGA GCAACAACCA GAAGCTGGAG
    GAATTGGATC TCAGCCACAA CGCCCTGGGA GACTTTGGAA TTAGACTTCT TTGTGTGGGG
    CTGAAGCATC TGTTCTGCAA TCTGAACAAG CTTTGGTTGG TGAATTCTGG CCTCACACCA
    GGTTGCTGTC CAGCTCTGGC CTCCATGCTC AGCACTAACC AGAAGCTCAC ACACCTTTAC
    CTACGGGGCA ATGCTCTTGG AGACACAGGG GTGAAGCTAC TCTGTGAGGG CCTCTTGCAC
    CCCAACTGCA AGCTTCAGAT ATTAGAATTG GATGGCTGTA GCCTCACCTC ACACTGTTGC
    TGGGATCTGT CCACACTTCT GACCTCCAGC AAGAGCCTCC GGGAGCTAAG CCTGGGCAGC
    AATGACCTGG GAGATCTAGG GGTCATGCTG CTCTGCGAAG TGCTAAAACA ACAGGGCTGC
    ATCCTACAAA GCCTGAAGTT GTGTGAAATG TATTTCAATT ATGATACAAA ATGTGCATTA
    GAAACACTTC AAGAAGAAAA GCCTGAGCTG ACCATTGTCT TTGAGCCTTT TGGTAGTGAC
    AGAAGCAGGC TGCCAGATGC CAGCGTTTGG GCTCCCTGCC ATCTAGGTGT CCACAAGTGG
    GAGCGAGCAC TCCTCCATCC AGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623206.1
    CDS319..3345
    Translation

    Target ORF information:

    RefSeq Version XM_005623149.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NLR family, pyrin domain containing 3 (NLRP3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623149.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCAAGCG TCCGCTGCAA GCTGGCTCGT TACCTGGAGG ACCTGGAAGA TGCAGACTTT 
    AAGAAATTCA AGATGCACTT ACAAGACTAC CCTTCTCAGA AAGGGTTCAG CCCTCTCCCT
    CGGAGTCAGA CAGAGAAAGC TGACCACATG GATCTGGCTA CTCTGATGAT CGACTTCAAT
    GGTGAGGAGA AGGCATGGGC CATGGCAGTG TGGATTTTTG CTGCAATCAA CAGAAGAGAC
    CTTTATGAGA AAGCTAAGAG GGATGAGCTG GAGTGGGATA ATTCACATCT GGAATGCCGA
    GAAGAAAGCC TTGAAGAGGA GTGGATGGGT TTATTGGGGT ACCTATCCAG AATCTCTATT
    TGTAAAAAAA AGAAAGATTA CTGCAAGAAG TACAAAAAAC ACGTGAGGAG CAGATTCCAG
    TGTATTAAAG ACAGGAATGC CCGTTTGGGT GAGACTGTGA ACCTCAACAA ACGCTACACT
    AGGCTGCGTC TTGTCAAGGA ACACCAGAGC CAGCAGGAGA GGGAGCATGA GCTCCTGGCC
    ATTGGCAGGA CTTCAGCCAA GACGCTGGAT AGCCCCATGA GTTCCGTGAA TGTGGAATTG
    CTGTTTGAGC CTGATGACCA ACACTTGGAG CCCGTTCACA CAGTGGTATT CCAGGGATCG
    GCAGGCATTG GGAAAACTAT ACTGGCCAGG AAGATCATGT TGGACTGGGC ATCAGACAAA
    ATTTACCAAG ACAGGTTTGA CTACTTGTTT TATATCCACT GTCGGGAGGT AAGCCTCGGG
    ACACGGAGGA GCCTGGGGGA CCTGATTGTC AGCTGCTGCC CTGACCCAAA CCCACCCATA
    TGTAAGATTG TGAGCAAGCC TTCCAGGATT CTCTTCCTTA TGGATGGCTT CGATGAGCTG
    CAGGGTGCCT TTGATGAGCA TACAGAGGCT CTCTGCACAA ACTGGCAGAA GGTGGAGCGG
    GGAGACATTC TCCTGAGCAG CCTCATCAGG AAGAGGCTGC TTCCTGAGGC CTCTTTACTC
    ATCACCACAA GACCTGTGGC TCTGGAGAAA CTGCAGCATT TGCTGGACCG TCCCCGTCAT
    GTGGAGATCC TGGGTTTCTC AGAGGCCAAA AGAAAGGAAT ATTTCTTCAA GTATTTCTCA
    GATGAGCAGC AAGCCACAGA AGCTTTCAGG CTGATTCAGG AGAATGAGAT CCTCTTTACC
    ATGTGCTTTA TCCCCTTGGT CTGCTGGATT GTGTGCACTG GGTTGAAGCA GCAAATGGAC
    AGTGGCAAGA ATCTTGCCCA GACATCCAAG ACTACCACTT CAGTGTATAT CTTCTTCCTC
    TCCAGTTTGT TGCAATCTCA CAGAGAGACC CAGAAGCACC AAGTCTCTGC CAGCCTCCGG
    GGTCTCTGCT CGTTGGCAGC AGATGGAATC TGGAACCAGA AAATCCTGTT TGATGAGTGT
    GACCTTAGGA ATCACGGCCT TCAGAAGGCA GATGTGTCTG CTTTCTTGAG AATGAACCTA
    TTCCAAAAGG AAGTGGACTG TGAGAAATTC TACAGCTTCA TCCACATGAC TTTCCAGGAG
    TTCTTTGCTG CCATGTACTA CCTTCTGGAA GAGGAGGAAC AGGGAAGGAT GAGGAACCTG
    CCATGGAGTA GTTCCAAGCT TCCCAACCGA GATGTGAAGG TTCTTCTTGA AAACTATGGC
    AAATTTGAAA AGGGATATCT GATTTTTGTT GTCCGTTTCC TCTTTGGCCT TGTAAATCAG
    GAGAGAACCT CCTACTTGGA GAAAAAATTG AGTTGCAAGA TCTCTCAACA AATCAGACTG
    GAGCTGCTCA AATGGATTGA AGAGAAAGCC AAGGGCAAGA ATCTGCAGAT CCAGCCCAGC
    CAGCTGGAGT TGTTCTATTG TTTGTATGAG ATGCAAGAGG AGGATTTTGT GCGAAAGGCC
    ATGGGCCATT TTCCCAAAAT TGAGATCAGT CTTTCCACCA GAATGGACCA TGTGGTTTCT
    TCCTTTTGTA TTGAGAACTG TTGCAGGGTG GAATCACTTT CCCTGAGGCT GCTTCATAAC
    TCCCCAAAGG AGGAAGAGGA GGAGGAGGAA GAGGAGAATG AGAAGGAAAT GCAACACTAT
    GATGTGGATC ACTGTGTCCT CCTAGATCCT CATACCACCT ATGCTTATCG GTTGGTGAAT
    TGCCAGGTCA CTGTCAGCCT TTGGCAGGGT CTCTTCTCAG TCCTGAGCAG AAACTCACTG
    AGTCTCACTG AACTGAATCT CAGTGACAAT GCTCTGGGGG ACCAAGGGGT AAATGTGTTA
    TGTGAAATGC TCCAGCATCC TGGCTGTAAT ATTCGGAAAT TGTGGTTGGG GCAGTGCTGC
    CTTTCCTATC GGTGCTGCTT CAACATCTCC TCGGTCCTGA GCAACAACCA GAAGCTGGAG
    GAATTGGATC TCAGCCACAA CGCCCTGGGA GACTTTGGAA TTAGACTTCT TTGTGTGGGG
    CTGAAGCATC TGTTCTGCAA TCTGAACAAG CTTTGGTTGG TGAATTCTGG CCTCACACCA
    GGTTGCTGTC CAGCTCTGGC CTCCATGCTC AGCACTAACC AGAAGCTCAC ACACCTTTAC
    CTACGGGGCA ATGCTCTTGG AGACACAGGG GTGAAGCTAC TCTGTGAGGG CCTCTTGCAC
    CCCAACTGCA AGCTTCAGAT ATTAGAATTG GATGGCTGTA GCCTCACCTC ACACTGTTGC
    TGGGATCTGT CCACACTTCT GACCTCCAGC AAGAGCCTCC GGGAGCTAAG CCTGGGCAGC
    AATGACCTGG GAGATCTAGG GGTCATGCTG CTCTGCGAAG TGCTAAAACA ACAGGGCTGC
    ATCCTACAAA GCCTGAAGTT GTGTGAAATG TATTTCAATT ATGATACAAA ATGTGCATTA
    GAAACACTTC AAGAAGAAAA GCCTGAGCTG ACCATTGTCT TTGAGCCTTT TGGTAGTGAC
    AGAAGCAGGC TGCCAGATGC CAGCGTTTGG GCTCCCTGCC ATCTAGGTGT CCACAAGTGG
    GAGCGAGCAC TCCTCCATCC AGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.