PIEZO1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following PIEZO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIEZO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe288455 XM_022419088.1
Latest version!
Canis lupus familiaris piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCe288456 XM_022419089.1
Latest version!
Canis lupus familiaris piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCe288457 XM_022419090.1
Latest version!
Canis lupus familiaris piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCe06942 XM_005620574.2
Latest version!
Canis lupus familiaris piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe06943 XM_005620575.1
Latest version!
Canis lupus familiaris piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe06944 XM_005620576.1
Latest version!
Canis lupus familiaris piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OCe06942 XM_005620574.1 Canis lupus familiaris piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe288455
    Clone ID Related Accession (Same CDS sequence) XM_022419088.1
    Accession Version XM_022419088.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product piezo-type mechanosensitive ion channel component 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGCTGCCCA CACAGGTCTT CCTGTTGTCC CCTCCTCTTA GCTTCCCTGC CACACCCAAG 
    CTCCTGATCT GGGTGAGGGC CTCGGGCCCC AGGACAAAGC CAGGATGGAG GACAGGCCAA
    GCCTGCCTGT TCCGCTATAA CGCGCTGTCT CTGGTCTATC TGCTCTTCCT GCTGCTGCTG
    CCCTGGTTCC CGGGCCCCTG TCGGCGCGGC TTCCCAGGCC ACACTCGTCG CCTGCTCCGG
    GCCCTGCTGG TTTTCAGCCT CATCTTCCTG GCAGCTCACC TGACCTTCCA GATATGCCTG
    CACACTGTGC CCCACCTGTA CCAGCTTCTG GAGCCCAGCT GTGGCCCCTG GGAGACCCTC
    TCTCGGCACA TTGGGGTCAC AAGGCTGGAC CTGAAGGACA TCCCCACTGC TGTCCGGCTG
    GTGGCCCCCG ACCTTGGTGT CCTGGTGGTC TGCGCTGTGT GCCTCGGCCT CTGCGGGCGC
    CTCACACAGG AAGCCCCACG GAGCCAGCGT GCCCAGGAGC TGGACGATGA TGATGATGGG
    GAAGTGGACA CCGGCTCCCC TGTGGGGCTT CAGGGAGCCC CCGTGCAGGC CCCCACGCGG
    AGGTCACGGT TGGCCACCCG GTTCCAGATC ACAGCCCACT GGCTGTTGGT GGCCGCGGGA
    AGGACCCTGG CTATTGTGCT GCTCGCATTG GCAGGCATCG CCCACCCCTC TGCCTTCTCC
    AGCGTCTACT TCCTAGTCTT CCTGGCCACC TGCACCTGGT GGGCATGCCA CTTTCCCATC
    AGCCTCCTGG GCTTCAGCAC GCTCTGCGTC ACAGTGGGCT GCTTCGGTGC CGGCCATCTC
    CTCTGCATCT ACTGCTACCA GACGCCCTTT GCCCAGACCG TGCTCCCTCC TGCTGGAATC
    TGGGCCAGGG TGTTTGGTCT CAAGGCCTTG GTGACCCACG GCAACTGCTC CAGCCCCAAC
    GTGCTGGTCT TCAGTACCAG CCACGACTGG CCCATCTACG TGAGCCCCGG CATCTTGCTG
    CTTCTCTACT ACACGGTGAC CTCACTCCTG AAGCTCCGCA CTCGCCAGCC GTTGGACCAG
    AGGAAGGAAG TGGCCAGGAA CGACGAGGAG CAGGAGGTAG AGCTGACCCA GGTGGACCAG
    TGGCTCCAGG ACCAGGCCAG AGCTGCAGCC ACCAAGGAGG AGGGGGCTGC CCAGCACATG
    CTGCCCACAA CCGTGGGGTC TGACTGGGAG ACCGACAGCT GTACGGTGCA TGACCTGACT
    GGCCACACCC CCGTCCGGCA GCGTCCAGTG CACCCCAGGC TGGCTGAGCC TCAAGAGACA
    TCTACCCTCC ACAGTCTGGG TCATCTCATT ATGGACCAGA GCTACGTGTG TGCCCTCATT
    GCCATGATGG TATGGAGCAT CACCTACCAT AGTTGGCTGA CCTTCGTGCT GCTGCTCTGG
    GCCTGCCTCA TCTGGACGGT GCGCAGCCGC CACCAGATGG CCATGCTCTG CTCACCCTTC
    ATCCTGCTCT ACGGGCTGGC TCTGTGTGGC CTGCGCTATG TGTGGGCCAT GGACCTGCGT
    CCCGAGCTGC CTACCACCCT GGGCCCCGTC AGCCTGCGTC AGCTGGGGCT GGAGCATACC
    CGCTACCCTT GCCTGGACCT TGGCGCCATG TTGCTCTACA CCCTGACCTT CTGGCTGCTG
    CTGCGCCGGT TTGTCAAGGA GAAGCTGCTG AAGAAGGGGA AGGTACCTGC TGCCCTGACA
    GAGGTCACTG TGGCTGACAC AGAGCCAACA CGGGCACGGA CGCTGCTGCA GAGCCTGGGG
    GAGCTGGTCA CAGGCCTGTA CGCCAAGTAC TGGATCTACG TGTGTGCCGG CATGTTCATT
    GTGGTCAGCT TCGCCGGCCG CCTCGTCGTC TACAAGATCG TGTACATGCT CCTTTTCCTG
    CTCTGCCTCA CCCTCTTCCA GGTCTACTAC AGCCTGTGGA GGAAGCTGCT CAAGGTGTTC
    TGGTGGCTGG TGGTGGCCTA TACCATGCTG GTGCTCATCG CCGTCTACAC TTTCCAGTTC
    CAGGACTTCC CCATGTACTG GCGCAACCTG ACGGGCCTCA CTGACGAACA GCTGGGGGAC
    CTGGGCCTGG AGCAGTTCAG CGTGTCGGAG CTTTTCTCCA GCATCCTGAT CCCGGGCTTC
    TTCCTGTTGG CCTGCATCCT GCAGCTGCAC TATTTCCACC GGCCGTTCAT GCAGCTCACG
    GACCTGGAGC ATGTGCCCCC GCCTGGTGCC TGCCCCCCAC GGTGGGCCCA CAGGCAGGAT
    GTGGTCAGTG GGACCCCGCT GCTGCAGGAG GAGGAGGACG GGCTGAGCAC CGCCTGCCCC
    CACCAGGCCA TGCAGGTCAC AGAAGCCAAC AAGTGGGGCC TGGTGGCGGA ACGGCTGCTG
    GACCTGGCTT CCGGCTTCTC GGACGTCCTC ACCCGTGTGC AGGTGCTGCT GCGGCGCCTT
    CTAGAGTTGC ACATCCTCAA GCTGGTGGCC CTGTACACTG TGTGGGTGGC CCTGAAGGAG
    GTGTCCGTGA TGAACCTGCT GCTGGTCGTG CTGTGGGCCT TCGCGCTGCC CTACCCCCGC
    TTCCGGCCCA TGGCCTCCTG CCTGTCCACT GTGTGGACCT GCATCATCAT CGTGTGCAAG
    ATGCTGTACC AGCTCAAGGT CGTAAGCCCG CACGAGTACT CCAGCAACTG CACCGAGCCC
    CTCCCCAACA GCACCAACCT GCAGAAGATG GAGATCAAGC AGTCCTTGCT ATACCGTGGG
    CCCGTTGACC CTGCCAACTG GTTTGGGGTG CGGAAGGGCT TCCCAAACCT AGGCTACATC
    CAGAACCACC TGCAGGTCCT GTTGCTGCTG GTGTTCGAGG CCATCGTCTA CCGGCGTCAG
    GAGCACCACC GTCGGCAGCA CCAGCTGGCC CCACTGCCCG CCCAGGCCGT CTGTGCCGAC
    GGCACCCGCC AGCGGCTGGA CCAGGACCTG CTCAGCTGCC TCAAGTACTT TGTCAACTTC
    TTTTTCTACA AATTTGGGCT AGAGATATGC TTCCTGATGG CCGTGAACGT GATTGGGCAG
    CGCATGAACT TCATGGTCAT CCTGCACGGC TGCTGGCTGG TGGCCATCCT CACACGGCGG
    CGCCGTGCAG CCATCGCCCG CCTCTGGCCC AACTACTGTC TCTTCCTGTC CCTGTTCTTG
    CTGTACCAGT ACCTGCTGTG CCTGGGCATC CCCCCGGCCC TGTGCATCGA CTACCCGTGG
    CGCTGGAGCC AGGCCATCCC CATGAACTCA GCACTCATCA AGTGGCTGTA CCTGCCCGAC
    TTCTTCAGAA CCCCCAATTC CACCAACCTC ATCAGCGACT TCCTGCTCCT GCTCTGCGCC
    TCCCAGCAGT GGCAGGTGTT CTCGGCTGAG CGCACGGAGG AATGGCAGCA CATGGCCGGC
    GTCAACACTG ACCGCCTGGA GCTGCCATTG GGTGAGCCCA ACGCCGTGCC CAACTTCATC
    TACTGCAGGT CCTACCTCGA CATGCTGAAG GTGGCTGTCT TCCGCTACCT CTTCTGGCTG
    GTGCTGGTAG TGGTATTTGT CACGGGAGCC ACCCGCGTCA GCGTCTTCGG GCTGGGCTAC
    CTGTTGGCCT GTTTCTATCT GCTGCTCTTC GGCACCAGCC TATTACAGAA GCACACACGC
    ACCCGCCTCG TGCTGTGGGA CTGCCTCATT CTCTACAACG TCACTGTCAT CATCTCTAAG
    AACATGCTCT CGCTCCTGTC CTGCGTTTTT GTGGAGCAAA TGCAGAGCAA CTTCTGCTGG
    GTTATCCAGC TTTTCAGCCT CGTGTGCACC GTCAAAGGCT ACTATGATCC CAAGGAGATG
    CTGAGCAGGG ACCGGGATTG CCTGCTGCCC GTGGAAGAGG CCGGTGTGCT CTGGGACAGC
    ATCTGCTTCC TGTTCCTTCT GCTGCAGCGG CGCGTCTTCC TCAGCCGCTA CTTCCTGCAT
    GTCAGCGCCG AGCTCCAGGC CACCGCCTTG CAGGCCTCCA GGGGCTTCGC GCTGTACAAT
    GCTGCCAACT TCAAAAGCAT CGATCTCCAC CGTAAGGCCG AGGAGAAGTC CCTGGCCCAG
    CTGAAAAGAC AGATGGAGCG CATCCGTGCC AAGCAAGAGA AGCACAGGCA GAGCCGAGCG
    GGCCGCAGCC GACCCCAGGA CACCCCAGAC CCCACCCAGG AGCCAGGGCC TGGCAGTCCA
    GGGGGCTCCT CCCTGCCACG GAGACAGTGG TGGCGGCCCT GGCTGGACCA CGCCACAGTC
    ATCCACTCTG GGGACTACTT CCTATTTGAG TCGGATAGTG AGGAGGAGGA GGAGGCCCTG
    CCCGAGGACC CCAGGCCGTC AGCACAGAGT GCCTTTCAGA TGGCGTACCA GGCATGGGTG
    ACCAATGCGC AGACAGTGCT GAGGCAGCAG CGGGAGGAGC AGGCGAGGCA GGACCAGACA
    GGGCAGTTGC CCACGGGAGG CGGCCCGGGA CAGGAGGCAG AGCCAGCGGA TGGCCCAGAC
    GAGGTGGTGG CTGGCCGCAG CCACGTGATG CAGCGAGTGC TGAGCACTGT GCAGTTTCTG
    TGGGTGCTGG GCCAAGCGCT GGTAGATGGG CTGACAGACT GGCTGCACAC CTTCACACGG
    CACCACCGCG CCATGAGTGA CGTGCTGCGC GCCGAGCGCT ACCTGTACAC ACAGGCGCTG
    GTTGAGGGCA GAGAGGTGCA CCGGAGCTTG CTGGACCAGC TCTACACTGG TGAAGCCGAG
    GCTGCCCCCG CAGTCCCCTT GGGGGTCCGA GATGCACCAA GTACAGCATC AAGTGGGCTG
    GGGGCTGAGG AGCCGCTGAG CACTGCGACA GAGGACACTG GCAGCCCCCT GAGCACCGGC
    TACCACACCC GCAGCAGCAG CAGCGAGGAG GTGGCCACTG AGCCTGGGGC TTCCCTACAC
    GGCTCTCGGG AGCTTCCTGC TGGCACTCCT ACCCGGATGC GCACAGCCAG TGAGCTGCTC
    CTCAGCAGGC ACCTCCGCAT CCAGGAGCTG GAGGAGGCCG AGCTGTTTGA AGCAGGGCAG
    GGCCGGGCAC TGCGGCTGCT GCAGGCCGCA TACCAGTGTG TGGCTGCCCA CTCAGAACTG
    CTCTGCTACT TCATCATCAT CCTCAACCAC ATGGTCACTG CCTCAGCCAC ATCCCTGGTG
    CTGCCTGTGC TCGTCTTCCT GTGGGCCATG CTGTCCATCC CCAGGCCCAG CAAGCGCTTC
    TGGATGACGG CCATCATCTT CACCGAGGTG TCTGTGGTCA CCAAGTACCT GTTCCAGTTT
    GGCTTCTTCC CCTGGAACAC ACACACAGTG CTCCGGCGCT ACGAGAACAA GCCGTACTTC
    CCACCGCGCA TCCTGGGTGT GGAGAAGACA GACAGCTACA TAAAGTATGA CCTGGTGCAG
    CTCATGGCCC TCTTCTTCCA CCGCTCCCAG CTGCTGTGCT ATGGTCTCTG GGATCATGAG
    GAGGACCCGC TGTCCAAGGA GCATGACCGT GGAAGTGAGA AGAAAGGGAC CGAGGAGGAA
    CAGGCACCAC TGGAACCCCA GACTGAGGAA GGCACAGGGC CCCAGGGCGA GCCAGTGGTG
    GCCGGGGCCC TCACCCAGGA CCACATCCAG GCAGAAGCAG GGGATGGGCC CCCAGAGCCT
    CCTGTGGAGC TCAAGCCCCG AGACATAAAG CGTATCAGCC TGCGTTTCAG GAAGAGGCGG
    AGGGAGACCA CAGAGCCTGT ACAACCCACA GCCACTGAAG GTGTGGTGGT GGCCTCCCGC
    AGAGAGAAGT GGAGTCGCCC CCGGGAAAGA ATGACAGCCA TGGGGCTCCG ACTGCAGACC
    TTCTGCCTGG CCGTGGCCCA GAGCATGTAC CGGCCCCTGC GGCGCTTCTT CGATGACATC
    TTGCACACCA AGTACCGTGC AGCCACCGAT GTCTACGCGC TCATGTTTCT GGCTGATGTG
    GTCGACTTCA TCATCATCAT CTTCGGCTTC TGGGCCTTTG GGAAACACTC GGCAGCCACA
    GACATCACAT CCTCGCTGTC GGACGACCAG GTGCCGGAGG CCTTCCTGGT CATGCTGCTC
    ATCCAGTTCA GCACCATGGT CATTGACCGT GCCCTCTACC TCCGCAAGAC CGTGCTGGGC
    AAGCTGGCCT TCCAGGTTGT CCTGGTGCTG GCCATCCACC TCTGGATGTT CTTTATTCTG
    CCCGCTGTCA CCGAGAGGAT GTTCAACCAG AACGCGGTGG CTCAGCTGTG GTACTTCGTG
    AAGTGCATCT ACTTCTCCCT GTCTGCCTAC CAGATCCGCT GTGGCTATCC GACCCGTATC
    CTTGGCAACT TCCTCACCAA GAAGTACAAT CACCTGAACC TCTTCCTCTT CCAGGGGTTC
    CGGCTTGTGC CATTCCTGGT GGAGCTGCGG GCCGTGATGG ACTGGGTATG GACAGACACC
    ACGCTGTCCC TGTCCAGCTG GATGTGTGTG GAGGACATCT ATGCCAACAT CTTCATCATC
    AAGTGCAGCC GTGAGACTGA GAAGAAATAT CCCCAGCCGA AGGGGCAGAA GAAGAAGAAG
    ATTGTCAAGT ACGGCATGGG CGGTCTCATC ATCCTGTTCC TCGTGGCTAT CATCTGGTTC
    CCGTTGCTTT TCATGTCCCT GGTACGCTCT GTGGTTGGTG TCGTCAACCA GCCCATCGAT
    GTTACTGTCA CCCTTAAGCT GGGTGGCTAT GAGCCCCTCT TTACCATGAG TGCCCAGCAG
    CCGTCCATCG TGCCGTTCAC ACAGCAGGCC TATGAGGAGC TGTCCAGACA GTTTGACCCT
    AACCCGTTGG CCATGCAGTT CATCAGCCAG TACAGCCCCG AGGACATCGT CACGGCACAG
    ATCGAGGGCA GTTCAGGGGC ACTGTGGCGC ATCAGCCCAC CCAGCCGGGC CCAGATGAAG
    CGGGAGCTAT ACAATGGTAC AGCTGACATC ACCCTGCGCT TCACCTGGAA CTTCCAGAGG
    GACCTGGCCA AGGGCGGCAC CGTGGAGTAC ACCAATGAGA AGCACACCCT GGACTGGGCC
    CCAAATAGCA CTGAGCGGCG GCAGCTGGCC AGCCTGCTTG AGGGCACCTC GGACCAGTCC
    GTGGTCATCC CCCACCTCTT TCCCAAGTAC ATCCGTGCCC CTAATGGGCC TGAAGCCAAC
    CCAGTGAAGC AGCTGCAGCC CAACGAGGAG GCCGACTACC TAGGCGTGCG CATCCAGCTG
    CGGAGGGAGC GCGTGGGCTC AGGGGCCGCT GGCTTCCTTG AGTGGTGGGT CATCGAGCTG
    CAGGATTGCC AGGCCGAGTG CAACCTGCTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCACCCAGCC TTGGCTTCCT GGCTGGCTAC GGGATCATGG GGCTCTATGT GTCCATCGTG
    CTGGTTATCG GCAAGTTTGT ACGCGGCTTC TTCAGTGAGA TCTCCCACTC CATCATGTTT
    GAGGAGCTGC CGTGTGTGGA CCGCATCCTC AAGCTCTGCC AGGACATCTT CCTGGTGCGG
    GAGACGCGGG AGCTGGAGCT GGAAGAGGAG CTTTATGCCA AGCTTATCTT CCTGTACCGC
    TCGCCTGAGA CGATGATCAA GTGGACCCGT GAGAAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274796.1
    CDS23..7624
    Translation

    Target ORF information:

    RefSeq Version XM_022419088.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419088.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGCTGCCCA CACAGGTCTT CCTGTTGTCC CCTCCTCTTA GCTTCCCTGC CACACCCAAG 
    CTCCTGATCT GGGTGAGGGC CTCGGGCCCC AGGACAAAGC CAGGATGGAG GACAGGCCAA
    GCCTGCCTGT TCCGCTATAA CGCGCTGTCT CTGGTCTATC TGCTCTTCCT GCTGCTGCTG
    CCCTGGTTCC CGGGCCCCTG TCGGCGCGGC TTCCCAGGCC ACACTCGTCG CCTGCTCCGG
    GCCCTGCTGG TTTTCAGCCT CATCTTCCTG GCAGCTCACC TGACCTTCCA GATATGCCTG
    CACACTGTGC CCCACCTGTA CCAGCTTCTG GAGCCCAGCT GTGGCCCCTG GGAGACCCTC
    TCTCGGCACA TTGGGGTCAC AAGGCTGGAC CTGAAGGACA TCCCCACTGC TGTCCGGCTG
    GTGGCCCCCG ACCTTGGTGT CCTGGTGGTC TGCGCTGTGT GCCTCGGCCT CTGCGGGCGC
    CTCACACAGG AAGCCCCACG GAGCCAGCGT GCCCAGGAGC TGGACGATGA TGATGATGGG
    GAAGTGGACA CCGGCTCCCC TGTGGGGCTT CAGGGAGCCC CCGTGCAGGC CCCCACGCGG
    AGGTCACGGT TGGCCACCCG GTTCCAGATC ACAGCCCACT GGCTGTTGGT GGCCGCGGGA
    AGGACCCTGG CTATTGTGCT GCTCGCATTG GCAGGCATCG CCCACCCCTC TGCCTTCTCC
    AGCGTCTACT TCCTAGTCTT CCTGGCCACC TGCACCTGGT GGGCATGCCA CTTTCCCATC
    AGCCTCCTGG GCTTCAGCAC GCTCTGCGTC ACAGTGGGCT GCTTCGGTGC CGGCCATCTC
    CTCTGCATCT ACTGCTACCA GACGCCCTTT GCCCAGACCG TGCTCCCTCC TGCTGGAATC
    TGGGCCAGGG TGTTTGGTCT CAAGGCCTTG GTGACCCACG GCAACTGCTC CAGCCCCAAC
    GTGCTGGTCT TCAGTACCAG CCACGACTGG CCCATCTACG TGAGCCCCGG CATCTTGCTG
    CTTCTCTACT ACACGGTGAC CTCACTCCTG AAGCTCCGCA CTCGCCAGCC GTTGGACCAG
    AGGAAGGAAG TGGCCAGGAA CGACGAGGAG CAGGAGGTAG AGCTGACCCA GGTGGACCAG
    TGGCTCCAGG ACCAGGCCAG AGCTGCAGCC ACCAAGGAGG AGGGGGCTGC CCAGCACATG
    CTGCCCACAA CCGTGGGGTC TGACTGGGAG ACCGACAGCT GTACGGTGCA TGACCTGACT
    GGCCACACCC CCGTCCGGCA GCGTCCAGTG CACCCCAGGC TGGCTGAGCC TCAAGAGACA
    TCTACCCTCC ACAGTCTGGG TCATCTCATT ATGGACCAGA GCTACGTGTG TGCCCTCATT
    GCCATGATGG TATGGAGCAT CACCTACCAT AGTTGGCTGA CCTTCGTGCT GCTGCTCTGG
    GCCTGCCTCA TCTGGACGGT GCGCAGCCGC CACCAGATGG CCATGCTCTG CTCACCCTTC
    ATCCTGCTCT ACGGGCTGGC TCTGTGTGGC CTGCGCTATG TGTGGGCCAT GGACCTGCGT
    CCCGAGCTGC CTACCACCCT GGGCCCCGTC AGCCTGCGTC AGCTGGGGCT GGAGCATACC
    CGCTACCCTT GCCTGGACCT TGGCGCCATG TTGCTCTACA CCCTGACCTT CTGGCTGCTG
    CTGCGCCGGT TTGTCAAGGA GAAGCTGCTG AAGAAGGGGA AGGTACCTGC TGCCCTGACA
    GAGGTCACTG TGGCTGACAC AGAGCCAACA CGGGCACGGA CGCTGCTGCA GAGCCTGGGG
    GAGCTGGTCA CAGGCCTGTA CGCCAAGTAC TGGATCTACG TGTGTGCCGG CATGTTCATT
    GTGGTCAGCT TCGCCGGCCG CCTCGTCGTC TACAAGATCG TGTACATGCT CCTTTTCCTG
    CTCTGCCTCA CCCTCTTCCA GGTCTACTAC AGCCTGTGGA GGAAGCTGCT CAAGGTGTTC
    TGGTGGCTGG TGGTGGCCTA TACCATGCTG GTGCTCATCG CCGTCTACAC TTTCCAGTTC
    CAGGACTTCC CCATGTACTG GCGCAACCTG ACGGGCCTCA CTGACGAACA GCTGGGGGAC
    CTGGGCCTGG AGCAGTTCAG CGTGTCGGAG CTTTTCTCCA GCATCCTGAT CCCGGGCTTC
    TTCCTGTTGG CCTGCATCCT GCAGCTGCAC TATTTCCACC GGCCGTTCAT GCAGCTCACG
    GACCTGGAGC ATGTGCCCCC GCCTGGTGCC TGCCCCCCAC GGTGGGCCCA CAGGCAGGAT
    GTGGTCAGTG GGACCCCGCT GCTGCAGGAG GAGGAGGACG GGCTGAGCAC CGCCTGCCCC
    CACCAGGCCA TGCAGGTCAC AGAAGCCAAC AAGTGGGGCC TGGTGGCGGA ACGGCTGCTG
    GACCTGGCTT CCGGCTTCTC GGACGTCCTC ACCCGTGTGC AGGTGCTGCT GCGGCGCCTT
    CTAGAGTTGC ACATCCTCAA GCTGGTGGCC CTGTACACTG TGTGGGTGGC CCTGAAGGAG
    GTGTCCGTGA TGAACCTGCT GCTGGTCGTG CTGTGGGCCT TCGCGCTGCC CTACCCCCGC
    TTCCGGCCCA TGGCCTCCTG CCTGTCCACT GTGTGGACCT GCATCATCAT CGTGTGCAAG
    ATGCTGTACC AGCTCAAGGT CGTAAGCCCG CACGAGTACT CCAGCAACTG CACCGAGCCC
    CTCCCCAACA GCACCAACCT GCAGAAGATG GAGATCAAGC AGTCCTTGCT ATACCGTGGG
    CCCGTTGACC CTGCCAACTG GTTTGGGGTG CGGAAGGGCT TCCCAAACCT AGGCTACATC
    CAGAACCACC TGCAGGTCCT GTTGCTGCTG GTGTTCGAGG CCATCGTCTA CCGGCGTCAG
    GAGCACCACC GTCGGCAGCA CCAGCTGGCC CCACTGCCCG CCCAGGCCGT CTGTGCCGAC
    GGCACCCGCC AGCGGCTGGA CCAGGACCTG CTCAGCTGCC TCAAGTACTT TGTCAACTTC
    TTTTTCTACA AATTTGGGCT AGAGATATGC TTCCTGATGG CCGTGAACGT GATTGGGCAG
    CGCATGAACT TCATGGTCAT CCTGCACGGC TGCTGGCTGG TGGCCATCCT CACACGGCGG
    CGCCGTGCAG CCATCGCCCG CCTCTGGCCC AACTACTGTC TCTTCCTGTC CCTGTTCTTG
    CTGTACCAGT ACCTGCTGTG CCTGGGCATC CCCCCGGCCC TGTGCATCGA CTACCCGTGG
    CGCTGGAGCC AGGCCATCCC CATGAACTCA GCACTCATCA AGTGGCTGTA CCTGCCCGAC
    TTCTTCAGAA CCCCCAATTC CACCAACCTC ATCAGCGACT TCCTGCTCCT GCTCTGCGCC
    TCCCAGCAGT GGCAGGTGTT CTCGGCTGAG CGCACGGAGG AATGGCAGCA CATGGCCGGC
    GTCAACACTG ACCGCCTGGA GCTGCCATTG GGTGAGCCCA ACGCCGTGCC CAACTTCATC
    TACTGCAGGT CCTACCTCGA CATGCTGAAG GTGGCTGTCT TCCGCTACCT CTTCTGGCTG
    GTGCTGGTAG TGGTATTTGT CACGGGAGCC ACCCGCGTCA GCGTCTTCGG GCTGGGCTAC
    CTGTTGGCCT GTTTCTATCT GCTGCTCTTC GGCACCAGCC TATTACAGAA GCACACACGC
    ACCCGCCTCG TGCTGTGGGA CTGCCTCATT CTCTACAACG TCACTGTCAT CATCTCTAAG
    AACATGCTCT CGCTCCTGTC CTGCGTTTTT GTGGAGCAAA TGCAGAGCAA CTTCTGCTGG
    GTTATCCAGC TTTTCAGCCT CGTGTGCACC GTCAAAGGCT ACTATGATCC CAAGGAGATG
    CTGAGCAGGG ACCGGGATTG CCTGCTGCCC GTGGAAGAGG CCGGTGTGCT CTGGGACAGC
    ATCTGCTTCC TGTTCCTTCT GCTGCAGCGG CGCGTCTTCC TCAGCCGCTA CTTCCTGCAT
    GTCAGCGCCG AGCTCCAGGC CACCGCCTTG CAGGCCTCCA GGGGCTTCGC GCTGTACAAT
    GCTGCCAACT TCAAAAGCAT CGATCTCCAC CGTAAGGCCG AGGAGAAGTC CCTGGCCCAG
    CTGAAAAGAC AGATGGAGCG CATCCGTGCC AAGCAAGAGA AGCACAGGCA GAGCCGAGCG
    GGCCGCAGCC GACCCCAGGA CACCCCAGAC CCCACCCAGG AGCCAGGGCC TGGCAGTCCA
    GGGGGCTCCT CCCTGCCACG GAGACAGTGG TGGCGGCCCT GGCTGGACCA CGCCACAGTC
    ATCCACTCTG GGGACTACTT CCTATTTGAG TCGGATAGTG AGGAGGAGGA GGAGGCCCTG
    CCCGAGGACC CCAGGCCGTC AGCACAGAGT GCCTTTCAGA TGGCGTACCA GGCATGGGTG
    ACCAATGCGC AGACAGTGCT GAGGCAGCAG CGGGAGGAGC AGGCGAGGCA GGACCAGACA
    GGGCAGTTGC CCACGGGAGG CGGCCCGGGA CAGGAGGCAG AGCCAGCGGA TGGCCCAGAC
    GAGGTGGTGG CTGGCCGCAG CCACGTGATG CAGCGAGTGC TGAGCACTGT GCAGTTTCTG
    TGGGTGCTGG GCCAAGCGCT GGTAGATGGG CTGACAGACT GGCTGCACAC CTTCACACGG
    CACCACCGCG CCATGAGTGA CGTGCTGCGC GCCGAGCGCT ACCTGTACAC ACAGGCGCTG
    GTTGAGGGCA GAGAGGTGCA CCGGAGCTTG CTGGACCAGC TCTACACTGG TGAAGCCGAG
    GCTGCCCCCG CAGTCCCCTT GGGGGTCCGA GATGCACCAA GTACAGCATC AAGTGGGCTG
    GGGGCTGAGG AGCCGCTGAG CACTGCGACA GAGGACACTG GCAGCCCCCT GAGCACCGGC
    TACCACACCC GCAGCAGCAG CAGCGAGGAG GTGGCCACTG AGCCTGGGGC TTCCCTACAC
    GGCTCTCGGG AGCTTCCTGC TGGCACTCCT ACCCGGATGC GCACAGCCAG TGAGCTGCTC
    CTCAGCAGGC ACCTCCGCAT CCAGGAGCTG GAGGAGGCCG AGCTGTTTGA AGCAGGGCAG
    GGCCGGGCAC TGCGGCTGCT GCAGGCCGCA TACCAGTGTG TGGCTGCCCA CTCAGAACTG
    CTCTGCTACT TCATCATCAT CCTCAACCAC ATGGTCACTG CCTCAGCCAC ATCCCTGGTG
    CTGCCTGTGC TCGTCTTCCT GTGGGCCATG CTGTCCATCC CCAGGCCCAG CAAGCGCTTC
    TGGATGACGG CCATCATCTT CACCGAGGTG TCTGTGGTCA CCAAGTACCT GTTCCAGTTT
    GGCTTCTTCC CCTGGAACAC ACACACAGTG CTCCGGCGCT ACGAGAACAA GCCGTACTTC
    CCACCGCGCA TCCTGGGTGT GGAGAAGACA GACAGCTACA TAAAGTATGA CCTGGTGCAG
    CTCATGGCCC TCTTCTTCCA CCGCTCCCAG CTGCTGTGCT ATGGTCTCTG GGATCATGAG
    GAGGACCCGC TGTCCAAGGA GCATGACCGT GGAAGTGAGA AGAAAGGGAC CGAGGAGGAA
    CAGGCACCAC TGGAACCCCA GACTGAGGAA GGCACAGGGC CCCAGGGCGA GCCAGTGGTG
    GCCGGGGCCC TCACCCAGGA CCACATCCAG GCAGAAGCAG GGGATGGGCC CCCAGAGCCT
    CCTGTGGAGC TCAAGCCCCG AGACATAAAG CGTATCAGCC TGCGTTTCAG GAAGAGGCGG
    AGGGAGACCA CAGAGCCTGT ACAACCCACA GCCACTGAAG GTGTGGTGGT GGCCTCCCGC
    AGAGAGAAGT GGAGTCGCCC CCGGGAAAGA ATGACAGCCA TGGGGCTCCG ACTGCAGACC
    TTCTGCCTGG CCGTGGCCCA GAGCATGTAC CGGCCCCTGC GGCGCTTCTT CGATGACATC
    TTGCACACCA AGTACCGTGC AGCCACCGAT GTCTACGCGC TCATGTTTCT GGCTGATGTG
    GTCGACTTCA TCATCATCAT CTTCGGCTTC TGGGCCTTTG GGAAACACTC GGCAGCCACA
    GACATCACAT CCTCGCTGTC GGACGACCAG GTGCCGGAGG CCTTCCTGGT CATGCTGCTC
    ATCCAGTTCA GCACCATGGT CATTGACCGT GCCCTCTACC TCCGCAAGAC CGTGCTGGGC
    AAGCTGGCCT TCCAGGTTGT CCTGGTGCTG GCCATCCACC TCTGGATGTT CTTTATTCTG
    CCCGCTGTCA CCGAGAGGAT GTTCAACCAG AACGCGGTGG CTCAGCTGTG GTACTTCGTG
    AAGTGCATCT ACTTCTCCCT GTCTGCCTAC CAGATCCGCT GTGGCTATCC GACCCGTATC
    CTTGGCAACT TCCTCACCAA GAAGTACAAT CACCTGAACC TCTTCCTCTT CCAGGGGTTC
    CGGCTTGTGC CATTCCTGGT GGAGCTGCGG GCCGTGATGG ACTGGGTATG GACAGACACC
    ACGCTGTCCC TGTCCAGCTG GATGTGTGTG GAGGACATCT ATGCCAACAT CTTCATCATC
    AAGTGCAGCC GTGAGACTGA GAAGAAATAT CCCCAGCCGA AGGGGCAGAA GAAGAAGAAG
    ATTGTCAAGT ACGGCATGGG CGGTCTCATC ATCCTGTTCC TCGTGGCTAT CATCTGGTTC
    CCGTTGCTTT TCATGTCCCT GGTACGCTCT GTGGTTGGTG TCGTCAACCA GCCCATCGAT
    GTTACTGTCA CCCTTAAGCT GGGTGGCTAT GAGCCCCTCT TTACCATGAG TGCCCAGCAG
    CCGTCCATCG TGCCGTTCAC ACAGCAGGCC TATGAGGAGC TGTCCAGACA GTTTGACCCT
    AACCCGTTGG CCATGCAGTT CATCAGCCAG TACAGCCCCG AGGACATCGT CACGGCACAG
    ATCGAGGGCA GTTCAGGGGC ACTGTGGCGC ATCAGCCCAC CCAGCCGGGC CCAGATGAAG
    CGGGAGCTAT ACAATGGTAC AGCTGACATC ACCCTGCGCT TCACCTGGAA CTTCCAGAGG
    GACCTGGCCA AGGGCGGCAC CGTGGAGTAC ACCAATGAGA AGCACACCCT GGACTGGGCC
    CCAAATAGCA CTGAGCGGCG GCAGCTGGCC AGCCTGCTTG AGGGCACCTC GGACCAGTCC
    GTGGTCATCC CCCACCTCTT TCCCAAGTAC ATCCGTGCCC CTAATGGGCC TGAAGCCAAC
    CCAGTGAAGC AGCTGCAGCC CAACGAGGAG GCCGACTACC TAGGCGTGCG CATCCAGCTG
    CGGAGGGAGC GCGTGGGCTC AGGGGCCGCT GGCTTCCTTG AGTGGTGGGT CATCGAGCTG
    CAGGATTGCC AGGCCGAGTG CAACCTGCTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCACCCAGCC TTGGCTTCCT GGCTGGCTAC GGGATCATGG GGCTCTATGT GTCCATCGTG
    CTGGTTATCG GCAAGTTTGT ACGCGGCTTC TTCAGTGAGA TCTCCCACTC CATCATGTTT
    GAGGAGCTGC CGTGTGTGGA CCGCATCCTC AAGCTCTGCC AGGACATCTT CCTGGTGCGG
    GAGACGCGGG AGCTGGAGCT GGAAGAGGAG CTTTATGCCA AGCTTATCTT CCTGTACCGC
    TCGCCTGAGA CGATGATCAA GTGGACCCGT GAGAAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288456
    Clone ID Related Accession (Same CDS sequence) XM_022419089.1
    Accession Version XM_022419089.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product piezo-type mechanosensitive ion channel component 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGCTGCCCA CACAGGTCTT CCTGTTGTCC CCTCCTCTTA GCTTCCCTGC CACACCCAAG 
    CTCCTGATCT GGGTGAGGGC CTCGGGCCCC AGGACAAAGC CAGGATGGAG GACAGGCCAA
    GCCTGCCTGT TCCGCTATAA CGCGCTGTCT CTGGTCTATC TGCTCTTCCT GCTGCTGCTG
    CCCTGGTTCC CGGGCCCCTG TCGGCGCGGC TTCCCAGGCC ACACTCGTCG CCTGCTCCGG
    GCCCTGCTGG TTTTCAGCCT CATCTTCCTG GCAGCTCACC TGACCTTCCA GATATGCCTG
    CACACTGTGC CCCACCTGTA CCAGCTTCTG GAGCCCAGCT GTGGCCCCTG GGAGACCCTC
    TCTCGGCACA TTGGGGTCAC AAGGCTGGAC CTGAAGGACA TCCCCACTGC TGTCCGGCTG
    GTGGCCCCCG ACCTTGGTGT CCTGGTGGTC TGCGCTGTGT GCCTCGGCCT CTGCGGGCGC
    CTCACACAGG AAGCCCCACG GAGCCAGCGT GCCCAGGAGC TGGACGATGA TGATGATGGG
    GAAGTGGACA CCGGCTCCCC TGTGGGGCTT CAGGGAGCCC CCGTGCAGGC CCCCACGCGG
    AGGTCACGGT TGGCCACCCG GTTCCAGATC ACAGCCCACT GGCTGTTGGT GGCCGCGGGA
    AGGACCCTGG CTATTGTGCT GCTCGCATTG GCAGGCATCG CCCACCCCTC TGCCTTCTCC
    AGCGTCTACT TCCTAGTCTT CCTGGCCACC TGCACCTGGT GGGCATGCCA CTTTCCCATC
    AGCCTCCTGG GCTTCAGCAC GCTCTGCGTC ACAGTGGGCT GCTTCGGTGC CGGCCATCTC
    CTCTGCATCT ACTGCTACCA GACGCCCTTT GCCCAGACCG TGCTCCCTCC TGCTGGAATC
    TGGGCCAGGG TGTTTGGTCT CAAGGCCTTG GTGACCCACG GCAACTGCTC CAGCCCCAAC
    GTGCTGGTCT TCAGTACCAG CCACGACTGG CCCATCTACG TGAGCCCCGG CATCTTGCTG
    CTTCTCTACT ACACGGTGAC CTCACTCCTG AAGCTCCGCA CTCGCCAGCC GTTGGACCAG
    AGGAAGGAAG TGGCCAGGAA CGACGAGGAG CAGGAGGTAG AGCTGACCCA GGTGGACCAG
    TGGCTCCAGG ACCAGGCCAG AGCTGCAGCC ACCAAGGAGG AGGGGGCTGC CCAGCACATG
    CTGCCCACAA CCGTGGGGTC TGACTGGGAG ACCGACAGCT GTACGGTGCA TGACCTGACT
    GGCCACACCC CCGTCCGGCA GCGTCCAGTG CACCCCAGGC TGGCTGAGCC TCAAGAGACA
    TCTACCCTCC ACAGTCTGGG TCATCTCATT ATGGACCAGA GCTACGTGTG TGCCCTCATT
    GCCATGATGG TATGGAGCAT CACCTACCAT AGTTGGCTGA CCTTCGTGCT GCTGCTCTGG
    GCCTGCCTCA TCTGGACGGT GCGCAGCCGC CACCAGATGG CCATGCTCTG CTCACCCTTC
    ATCCTGCTCT ACGGGCTGGC TCTGTGTGGC CTGCGCTATG TGTGGGCCAT GGACCTGCGT
    CCCGAGCTGC CTACCACCCT GGGCCCCGTC AGCCTGCGTC AGCTGGGGCT GGAGCATACC
    CGCTACCCTT GCCTGGACCT TGGCGCCATG TTGCTCTACA CCCTGACCTT CTGGCTGCTG
    CTGCGCCGGT TTGTCAAGGA GAAGCTGCTG AAGAAGGGGA AGGTACCTGC TGCCCTGACA
    GAGGTCACTG TGGCTGACAC AGAGCCAACA CGGGCACGGA CGCTGCTGCA GAGCCTGGGG
    GAGCTGGTCA CAGGCCTGTA CGCCAAGTAC TGGATCTACG TGTGTGCCGG CATGTTCATT
    GTGGTCAGCT TCGCCGGCCG CCTCGTCGTC TACAAGATCG TGTACATGCT CCTTTTCCTG
    CTCTGCCTCA CCCTCTTCCA GGTCTACTAC AGCCTGTGGA GGAAGCTGCT CAAGGTGTTC
    TGGTGGCTGG TGGTGGCCTA TACCATGCTG GTGCTCATCG CCGTCTACAC TTTCCAGTTC
    CAGGACTTCC CCATGTACTG GCGCAACCTG ACGGGCCTCA CTGACGAACA GCTGGGGGAC
    CTGGGCCTGG AGCAGTTCAG CGTGTCGGAG CTTTTCTCCA GCATCCTGAT CCCGGGCTTC
    TTCCTGTTGG CCTGCATCCT GCAGCTGCAC TATTTCCACC GGCCGTTCAT GCAGCTCACG
    GACCTGGAGC ATGTGCCCCC GCCTGGTGCC TGCCCCCCAC GGTGGGCCCA CAGGCAGGAT
    GTGGTCAGTG GGACCCCGCT GCTGCAGGAG GAGGAGGACG GGCTGAGCAC CGCCTGCCCC
    CACCAGGCCA TGCAGGTCAC AGAAGCCAAC AAGTGGGGCC TGGTGGCGGA ACGGCTGCTG
    GACCTGGCTT CCGGCTTCTC GGACGTCCTC ACCCGTGTGC AGGTGCTGCT GCGGCGCCTT
    CTAGAGTTGC ACATCCTCAA GCTGGTGGCC CTGTACACTG TGTGGGTGGC CCTGAAGGAG
    GTGTCCGTGA TGAACCTGCT GCTGGTCGTG CTGTGGGCCT TCGCGCTGCC CTACCCCCGC
    TTCCGGCCCA TGGCCTCCTG CCTGTCCACT GTGTGGACCT GCATCATCAT CGTGTGCAAG
    ATGCTGTACC AGCTCAAGGT CGTAAGCCCG CACGAGTACT CCAGCAACTG CACCGAGCCC
    CTCCCCAACA GCACCAACCT GCAGAAGATG GAGATCAAGC AGTCCTTGCT ATACCGTGGG
    CCCGTTGACC CTGCCAACTG GTTTGGGGTG CGGAAGGGCT TCCCAAACCT AGGCTACATC
    CAGAACCACC TGCAGGTCCT GTTGCTGCTG GTGTTCGAGG CCATCGTCTA CCGGCGTCAG
    GAGCACCACC GTCGGCAGCA CCAGCTGGCC CCACTGCCCG CCCAGGCCGT CTGTGCCGAC
    GGCACCCGCC AGCGGCTGGA CCAGGACCTG CTCAGCTGCC TCAAGTACTT TGTCAACTTC
    TTTTTCTACA AATTTGGGCT AGAGATATGC TTCCTGATGG CCGTGAACGT GATTGGGCAG
    CGCATGAACT TCATGGTCAT CCTGCACGGC TGCTGGCTGG TGGCCATCCT CACACGGCGG
    CGCCGTGCAG CCATCGCCCG CCTCTGGCCC AACTACTGTC TCTTCCTGTC CCTGTTCTTG
    CTGTACCAGT ACCTGCTGTG CCTGGGCATC CCCCCGGCCC TGTGCATCGA CTACCCGTGG
    CGCTGGAGCC AGGCCATCCC CATGAACTCA GCACTCATCA AGTGGCTGTA CCTGCCCGAC
    TTCTTCAGAA CCCCCAATTC CACCAACCTC ATCAGCGACT TCCTGCTCCT GCTCTGCGCC
    TCCCAGCAGT GGCAGGTGTT CTCGGCTGAG CGCACGGAGG AATGGCAGCA CATGGCCGGC
    GTCAACACTG ACCGCCTGGA GCTGCCATTG GGTGAGCCCA ACGCCGTGCC CAACTTCATC
    TACTGCAGGT CCTACCTCGA CATGCTGAAG GTGGCTGTCT TCCGCTACCT CTTCTGGCTG
    GTGCTGGTAG TGGTATTTGT CACGGGAGCC ACCCGCGTCA GCGTCTTCGG GCTGGGCTAC
    CTGTTGGCCT GTTTCTATCT GCTGCTCTTC GGCACCAGCC TATTACAGAA GCACACACGC
    ACCCGCCTCG TGCTGTGGGA CTGCCTCATT CTCTACAACG TCACTGTCAT CATCTCTAAG
    AACATGCTCT CGCTCCTGTC CTGCGTTTTT GTGGAGCAAA TGCAGAGCAA CTTCTGCTGG
    GTTATCCAGC TTTTCAGCCT CGTGTGCACC GTCAAAGGCT ACTATGATCC CAAGGAGATG
    CTGAGCAGGG ACCGGGATTG CCTGCTGCCC GTGGAAGAGG CCGGTGTGCT CTGGGACAGC
    ATCTGCTTCC TGTTCCTTCT GCTGCAGCGG CGCGTCTTCC TCAGCCGCTA CTTCCTGCAT
    GTCAGCGCCG AGCTCCAGGC CACCGCCTTG CAGGCCTCCA GGGGCTTCGC GCTGTACAAT
    GCTGCCAACT TCAAAAGCAT CGATCTCCAC CGTAAGGCCG AGGAGAAGTC CCTGGCCCAG
    CTGAAAAGAC AGATGGAGCG CATCCGTGCC AAGCAAGAGA AGCACAGGCA GAGCCGAGCG
    GGCCGCAGCC GACCCCAGGA CACCCCAGAC CCCACCCAGG AGCCAGGGCC TGGCAGTCCA
    GGGGGCTCCT CCCTGCCACG GAGACAGTGG TGGCGGCCCT GGCTGGACCA CGCCACAGTC
    ATCCACTCTG GGGACTACTT CCTATTTGAG TCGGATAGTG AGGAGGAGGA GGAGGCCCTG
    CCCGAGGACC CCAGGCCGTC AGCACAGAGT GCCTTTCAGA TGGCGTACCA GGCATGGGTG
    ACCAATGCGC AGACAGTGCT GAGGCAGCAG CGGGAGGAGC AGGCGAGGCA GGACCAGACA
    GGGCAGTTGC CCACGGGAGG CGGCCCGGGA CAGGAGGCAG AGCCAGCGGA TGGCCCAGAC
    GAGGTGGTGG CTGGCCGCAG CCACGTGATG CAGCGAGTGC TGAGCACTGT GCAGTTTCTG
    TGGGTGCTGG GCCAAGCGCT GGTAGATGGG CTGACAGACT GGCTGCACAC CTTCACACGG
    CACCACCGCG CCATGAGTGA CGTGCTGCGC GCCGAGCGCT ACCTGTACAC ACAGGCGCTG
    GTTGAGGGCA GAGAGGTGCA CCGGAGCTTG CTGGACCAGC TCTACACTGG TGAAGCCGAG
    GCTGCCCCCG CAGTCCCCTT GGGGGTCCGA GATGCACCAA GTACAGCATC AAGTGGGCTG
    GGGGCTGAGG AGCCGCTGAG CACTGCGACA GAGGACACTG GCAGCCCCCT GAGCACCGGC
    TACCACACCC GCAGCAGCAG CAGCGAGGAG GTGGCCACTG AGCCTGGGGC TTCCCTACAC
    GGCTCTCGGG AGCTTCCTGC TGGCACTCCT ACCCGGATGC GCACAGCCAG TGAGCTGCTC
    CTCAGCAGGC ACCTCCGCAT CCAGGAGCTG GAGGAGGCCG AGCTGTTTGA AGCAGGGCAG
    GGCCGGGCAC TGCGGCTGCT GCAGGCCGCA TACCAGTGTG TGGCTGCCCA CTCAGAACTG
    CTCTGCTACT TCATCATCAT CCTCAACCAC ATGGTCACTG CCTCAGCCAC ATCCCTGGTG
    CTGCCTGTGC TCGTCTTCCT GTGGGCCATG CTGTCCATCC CCAGGCCCAG CAAGCGCTTC
    TGGATGACGG CCATCATCTT CACCGAGGTG TCTGTGGTCA CCAAGTACCT GTTCCAGTTT
    GGCTTCTTCC CCTGGAACAC ACACACAGTG CTCCGGCGCT ACGAGAACAA GCCGTACTTC
    CCACCGCGCA TCCTGGGTGT GGAGAAGACA GACAGCTACA TAAAGTATGA CCTGGTGCAG
    CTCATGGCCC TCTTCTTCCA CCGCTCCCAG CTGCTGTGCT ATGGTCTCTG GGATCATGAG
    GAGGACCCGC TGTCCAAGGA GCATGACCGT GGAAGTGAGA AGAAAGGGAC CGAGGAGGAA
    CAGGCACCAC TGGAACCCCA GACTGAGGAA GGCACAGGGC CCCAGGGCGA GCCAGTGGTG
    GCCGGGGCCC TCACCCAGGA CCACATCCAG GCAGAAGCAG GGGATGGGCC CCCAGAGCCT
    CCTGTGGAGC TCAAGCCCCG AGACATAAAG CGTATCAGCC TGCGTTTCAG GAAGAGGCGG
    AGGGAGACCA CAGAGCCTGT ACAACCCACA GCCACTGGTG TGGTGGTGGC CTCCCGCAGA
    GAGAAGTGGA GTCGCCCCCG GGAAAGAATG ACAGCCATGG GGCTCCGACT GCAGACCTTC
    TGCCTGGCCG TGGCCCAGAG CATGTACCGG CCCCTGCGGC GCTTCTTCGA TGACATCTTG
    CACACCAAGT ACCGTGCAGC CACCGATGTC TACGCGCTCA TGTTTCTGGC TGATGTGGTC
    GACTTCATCA TCATCATCTT CGGCTTCTGG GCCTTTGGGA AACACTCGGC AGCCACAGAC
    ATCACATCCT CGCTGTCGGA CGACCAGGTG CCGGAGGCCT TCCTGGTCAT GCTGCTCATC
    CAGTTCAGCA CCATGGTCAT TGACCGTGCC CTCTACCTCC GCAAGACCGT GCTGGGCAAG
    CTGGCCTTCC AGGTTGTCCT GGTGCTGGCC ATCCACCTCT GGATGTTCTT TATTCTGCCC
    GCTGTCACCG AGAGGATGTT CAACCAGAAC GCGGTGGCTC AGCTGTGGTA CTTCGTGAAG
    TGCATCTACT TCTCCCTGTC TGCCTACCAG ATCCGCTGTG GCTATCCGAC CCGTATCCTT
    GGCAACTTCC TCACCAAGAA GTACAATCAC CTGAACCTCT TCCTCTTCCA GGGGTTCCGG
    CTTGTGCCAT TCCTGGTGGA GCTGCGGGCC GTGATGGACT GGGTATGGAC AGACACCACG
    CTGTCCCTGT CCAGCTGGAT GTGTGTGGAG GACATCTATG CCAACATCTT CATCATCAAG
    TGCAGCCGTG AGACTGAGAA GAAATATCCC CAGCCGAAGG GGCAGAAGAA GAAGAAGATT
    GTCAAGTACG GCATGGGCGG TCTCATCATC CTGTTCCTCG TGGCTATCAT CTGGTTCCCG
    TTGCTTTTCA TGTCCCTGGT ACGCTCTGTG GTTGGTGTCG TCAACCAGCC CATCGATGTT
    ACTGTCACCC TTAAGCTGGG TGGCTATGAG CCCCTCTTTA CCATGAGTGC CCAGCAGCCG
    TCCATCGTGC CGTTCACACA GCAGGCCTAT GAGGAGCTGT CCAGACAGTT TGACCCTAAC
    CCGTTGGCCA TGCAGTTCAT CAGCCAGTAC AGCCCCGAGG ACATCGTCAC GGCACAGATC
    GAGGGCAGTT CAGGGGCACT GTGGCGCATC AGCCCACCCA GCCGGGCCCA GATGAAGCGG
    GAGCTATACA ATGGTACAGC TGACATCACC CTGCGCTTCA CCTGGAACTT CCAGAGGGAC
    CTGGCCAAGG GCGGCACCGT GGAGTACACC AATGAGAAGC ACACCCTGGA CTGGGCCCCA
    AATAGCACTG AGCGGCGGCA GCTGGCCAGC CTGCTTGAGG GCACCTCGGA CCAGTCCGTG
    GTCATCCCCC ACCTCTTTCC CAAGTACATC CGTGCCCCTA ATGGGCCTGA AGCCAACCCA
    GTGAAGCAGC TGCAGCCCAA CGAGGAGGCC GACTACCTAG GCGTGCGCAT CCAGCTGCGG
    AGGGAGCGCG TGGGCTCAGG GGCCGCTGGC TTCCTTGAGT GGTGGGTCAT CGAGCTGCAG
    GATTGCCAGG CCGAGTGCAA CCTGCTGCCC ATGGTCATCT TCAGTGACAA GGTCAGCCCA
    CCCAGCCTTG GCTTCCTGGC TGGCTACGGG ATCATGGGGC TCTATGTGTC CATCGTGCTG
    GTTATCGGCA AGTTTGTACG CGGCTTCTTC AGTGAGATCT CCCACTCCAT CATGTTTGAG
    GAGCTGCCGT GTGTGGACCG CATCCTCAAG CTCTGCCAGG ACATCTTCCT GGTGCGGGAG
    ACGCGGGAGC TGGAGCTGGA AGAGGAGCTT TATGCCAAGC TTATCTTCCT GTACCGCTCG
    CCTGAGACGA TGATCAAGTG GACCCGTGAG AAGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274797.1
    CDS25..7623
    Translation

    Target ORF information:

    RefSeq Version XM_022419089.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419089.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGCTGCCCA CACAGGTCTT CCTGTTGTCC CCTCCTCTTA GCTTCCCTGC CACACCCAAG 
    CTCCTGATCT GGGTGAGGGC CTCGGGCCCC AGGACAAAGC CAGGATGGAG GACAGGCCAA
    GCCTGCCTGT TCCGCTATAA CGCGCTGTCT CTGGTCTATC TGCTCTTCCT GCTGCTGCTG
    CCCTGGTTCC CGGGCCCCTG TCGGCGCGGC TTCCCAGGCC ACACTCGTCG CCTGCTCCGG
    GCCCTGCTGG TTTTCAGCCT CATCTTCCTG GCAGCTCACC TGACCTTCCA GATATGCCTG
    CACACTGTGC CCCACCTGTA CCAGCTTCTG GAGCCCAGCT GTGGCCCCTG GGAGACCCTC
    TCTCGGCACA TTGGGGTCAC AAGGCTGGAC CTGAAGGACA TCCCCACTGC TGTCCGGCTG
    GTGGCCCCCG ACCTTGGTGT CCTGGTGGTC TGCGCTGTGT GCCTCGGCCT CTGCGGGCGC
    CTCACACAGG AAGCCCCACG GAGCCAGCGT GCCCAGGAGC TGGACGATGA TGATGATGGG
    GAAGTGGACA CCGGCTCCCC TGTGGGGCTT CAGGGAGCCC CCGTGCAGGC CCCCACGCGG
    AGGTCACGGT TGGCCACCCG GTTCCAGATC ACAGCCCACT GGCTGTTGGT GGCCGCGGGA
    AGGACCCTGG CTATTGTGCT GCTCGCATTG GCAGGCATCG CCCACCCCTC TGCCTTCTCC
    AGCGTCTACT TCCTAGTCTT CCTGGCCACC TGCACCTGGT GGGCATGCCA CTTTCCCATC
    AGCCTCCTGG GCTTCAGCAC GCTCTGCGTC ACAGTGGGCT GCTTCGGTGC CGGCCATCTC
    CTCTGCATCT ACTGCTACCA GACGCCCTTT GCCCAGACCG TGCTCCCTCC TGCTGGAATC
    TGGGCCAGGG TGTTTGGTCT CAAGGCCTTG GTGACCCACG GCAACTGCTC CAGCCCCAAC
    GTGCTGGTCT TCAGTACCAG CCACGACTGG CCCATCTACG TGAGCCCCGG CATCTTGCTG
    CTTCTCTACT ACACGGTGAC CTCACTCCTG AAGCTCCGCA CTCGCCAGCC GTTGGACCAG
    AGGAAGGAAG TGGCCAGGAA CGACGAGGAG CAGGAGGTAG AGCTGACCCA GGTGGACCAG
    TGGCTCCAGG ACCAGGCCAG AGCTGCAGCC ACCAAGGAGG AGGGGGCTGC CCAGCACATG
    CTGCCCACAA CCGTGGGGTC TGACTGGGAG ACCGACAGCT GTACGGTGCA TGACCTGACT
    GGCCACACCC CCGTCCGGCA GCGTCCAGTG CACCCCAGGC TGGCTGAGCC TCAAGAGACA
    TCTACCCTCC ACAGTCTGGG TCATCTCATT ATGGACCAGA GCTACGTGTG TGCCCTCATT
    GCCATGATGG TATGGAGCAT CACCTACCAT AGTTGGCTGA CCTTCGTGCT GCTGCTCTGG
    GCCTGCCTCA TCTGGACGGT GCGCAGCCGC CACCAGATGG CCATGCTCTG CTCACCCTTC
    ATCCTGCTCT ACGGGCTGGC TCTGTGTGGC CTGCGCTATG TGTGGGCCAT GGACCTGCGT
    CCCGAGCTGC CTACCACCCT GGGCCCCGTC AGCCTGCGTC AGCTGGGGCT GGAGCATACC
    CGCTACCCTT GCCTGGACCT TGGCGCCATG TTGCTCTACA CCCTGACCTT CTGGCTGCTG
    CTGCGCCGGT TTGTCAAGGA GAAGCTGCTG AAGAAGGGGA AGGTACCTGC TGCCCTGACA
    GAGGTCACTG TGGCTGACAC AGAGCCAACA CGGGCACGGA CGCTGCTGCA GAGCCTGGGG
    GAGCTGGTCA CAGGCCTGTA CGCCAAGTAC TGGATCTACG TGTGTGCCGG CATGTTCATT
    GTGGTCAGCT TCGCCGGCCG CCTCGTCGTC TACAAGATCG TGTACATGCT CCTTTTCCTG
    CTCTGCCTCA CCCTCTTCCA GGTCTACTAC AGCCTGTGGA GGAAGCTGCT CAAGGTGTTC
    TGGTGGCTGG TGGTGGCCTA TACCATGCTG GTGCTCATCG CCGTCTACAC TTTCCAGTTC
    CAGGACTTCC CCATGTACTG GCGCAACCTG ACGGGCCTCA CTGACGAACA GCTGGGGGAC
    CTGGGCCTGG AGCAGTTCAG CGTGTCGGAG CTTTTCTCCA GCATCCTGAT CCCGGGCTTC
    TTCCTGTTGG CCTGCATCCT GCAGCTGCAC TATTTCCACC GGCCGTTCAT GCAGCTCACG
    GACCTGGAGC ATGTGCCCCC GCCTGGTGCC TGCCCCCCAC GGTGGGCCCA CAGGCAGGAT
    GTGGTCAGTG GGACCCCGCT GCTGCAGGAG GAGGAGGACG GGCTGAGCAC CGCCTGCCCC
    CACCAGGCCA TGCAGGTCAC AGAAGCCAAC AAGTGGGGCC TGGTGGCGGA ACGGCTGCTG
    GACCTGGCTT CCGGCTTCTC GGACGTCCTC ACCCGTGTGC AGGTGCTGCT GCGGCGCCTT
    CTAGAGTTGC ACATCCTCAA GCTGGTGGCC CTGTACACTG TGTGGGTGGC CCTGAAGGAG
    GTGTCCGTGA TGAACCTGCT GCTGGTCGTG CTGTGGGCCT TCGCGCTGCC CTACCCCCGC
    TTCCGGCCCA TGGCCTCCTG CCTGTCCACT GTGTGGACCT GCATCATCAT CGTGTGCAAG
    ATGCTGTACC AGCTCAAGGT CGTAAGCCCG CACGAGTACT CCAGCAACTG CACCGAGCCC
    CTCCCCAACA GCACCAACCT GCAGAAGATG GAGATCAAGC AGTCCTTGCT ATACCGTGGG
    CCCGTTGACC CTGCCAACTG GTTTGGGGTG CGGAAGGGCT TCCCAAACCT AGGCTACATC
    CAGAACCACC TGCAGGTCCT GTTGCTGCTG GTGTTCGAGG CCATCGTCTA CCGGCGTCAG
    GAGCACCACC GTCGGCAGCA CCAGCTGGCC CCACTGCCCG CCCAGGCCGT CTGTGCCGAC
    GGCACCCGCC AGCGGCTGGA CCAGGACCTG CTCAGCTGCC TCAAGTACTT TGTCAACTTC
    TTTTTCTACA AATTTGGGCT AGAGATATGC TTCCTGATGG CCGTGAACGT GATTGGGCAG
    CGCATGAACT TCATGGTCAT CCTGCACGGC TGCTGGCTGG TGGCCATCCT CACACGGCGG
    CGCCGTGCAG CCATCGCCCG CCTCTGGCCC AACTACTGTC TCTTCCTGTC CCTGTTCTTG
    CTGTACCAGT ACCTGCTGTG CCTGGGCATC CCCCCGGCCC TGTGCATCGA CTACCCGTGG
    CGCTGGAGCC AGGCCATCCC CATGAACTCA GCACTCATCA AGTGGCTGTA CCTGCCCGAC
    TTCTTCAGAA CCCCCAATTC CACCAACCTC ATCAGCGACT TCCTGCTCCT GCTCTGCGCC
    TCCCAGCAGT GGCAGGTGTT CTCGGCTGAG CGCACGGAGG AATGGCAGCA CATGGCCGGC
    GTCAACACTG ACCGCCTGGA GCTGCCATTG GGTGAGCCCA ACGCCGTGCC CAACTTCATC
    TACTGCAGGT CCTACCTCGA CATGCTGAAG GTGGCTGTCT TCCGCTACCT CTTCTGGCTG
    GTGCTGGTAG TGGTATTTGT CACGGGAGCC ACCCGCGTCA GCGTCTTCGG GCTGGGCTAC
    CTGTTGGCCT GTTTCTATCT GCTGCTCTTC GGCACCAGCC TATTACAGAA GCACACACGC
    ACCCGCCTCG TGCTGTGGGA CTGCCTCATT CTCTACAACG TCACTGTCAT CATCTCTAAG
    AACATGCTCT CGCTCCTGTC CTGCGTTTTT GTGGAGCAAA TGCAGAGCAA CTTCTGCTGG
    GTTATCCAGC TTTTCAGCCT CGTGTGCACC GTCAAAGGCT ACTATGATCC CAAGGAGATG
    CTGAGCAGGG ACCGGGATTG CCTGCTGCCC GTGGAAGAGG CCGGTGTGCT CTGGGACAGC
    ATCTGCTTCC TGTTCCTTCT GCTGCAGCGG CGCGTCTTCC TCAGCCGCTA CTTCCTGCAT
    GTCAGCGCCG AGCTCCAGGC CACCGCCTTG CAGGCCTCCA GGGGCTTCGC GCTGTACAAT
    GCTGCCAACT TCAAAAGCAT CGATCTCCAC CGTAAGGCCG AGGAGAAGTC CCTGGCCCAG
    CTGAAAAGAC AGATGGAGCG CATCCGTGCC AAGCAAGAGA AGCACAGGCA GAGCCGAGCG
    GGCCGCAGCC GACCCCAGGA CACCCCAGAC CCCACCCAGG AGCCAGGGCC TGGCAGTCCA
    GGGGGCTCCT CCCTGCCACG GAGACAGTGG TGGCGGCCCT GGCTGGACCA CGCCACAGTC
    ATCCACTCTG GGGACTACTT CCTATTTGAG TCGGATAGTG AGGAGGAGGA GGAGGCCCTG
    CCCGAGGACC CCAGGCCGTC AGCACAGAGT GCCTTTCAGA TGGCGTACCA GGCATGGGTG
    ACCAATGCGC AGACAGTGCT GAGGCAGCAG CGGGAGGAGC AGGCGAGGCA GGACCAGACA
    GGGCAGTTGC CCACGGGAGG CGGCCCGGGA CAGGAGGCAG AGCCAGCGGA TGGCCCAGAC
    GAGGTGGTGG CTGGCCGCAG CCACGTGATG CAGCGAGTGC TGAGCACTGT GCAGTTTCTG
    TGGGTGCTGG GCCAAGCGCT GGTAGATGGG CTGACAGACT GGCTGCACAC CTTCACACGG
    CACCACCGCG CCATGAGTGA CGTGCTGCGC GCCGAGCGCT ACCTGTACAC ACAGGCGCTG
    GTTGAGGGCA GAGAGGTGCA CCGGAGCTTG CTGGACCAGC TCTACACTGG TGAAGCCGAG
    GCTGCCCCCG CAGTCCCCTT GGGGGTCCGA GATGCACCAA GTACAGCATC AAGTGGGCTG
    GGGGCTGAGG AGCCGCTGAG CACTGCGACA GAGGACACTG GCAGCCCCCT GAGCACCGGC
    TACCACACCC GCAGCAGCAG CAGCGAGGAG GTGGCCACTG AGCCTGGGGC TTCCCTACAC
    GGCTCTCGGG AGCTTCCTGC TGGCACTCCT ACCCGGATGC GCACAGCCAG TGAGCTGCTC
    CTCAGCAGGC ACCTCCGCAT CCAGGAGCTG GAGGAGGCCG AGCTGTTTGA AGCAGGGCAG
    GGCCGGGCAC TGCGGCTGCT GCAGGCCGCA TACCAGTGTG TGGCTGCCCA CTCAGAACTG
    CTCTGCTACT TCATCATCAT CCTCAACCAC ATGGTCACTG CCTCAGCCAC ATCCCTGGTG
    CTGCCTGTGC TCGTCTTCCT GTGGGCCATG CTGTCCATCC CCAGGCCCAG CAAGCGCTTC
    TGGATGACGG CCATCATCTT CACCGAGGTG TCTGTGGTCA CCAAGTACCT GTTCCAGTTT
    GGCTTCTTCC CCTGGAACAC ACACACAGTG CTCCGGCGCT ACGAGAACAA GCCGTACTTC
    CCACCGCGCA TCCTGGGTGT GGAGAAGACA GACAGCTACA TAAAGTATGA CCTGGTGCAG
    CTCATGGCCC TCTTCTTCCA CCGCTCCCAG CTGCTGTGCT ATGGTCTCTG GGATCATGAG
    GAGGACCCGC TGTCCAAGGA GCATGACCGT GGAAGTGAGA AGAAAGGGAC CGAGGAGGAA
    CAGGCACCAC TGGAACCCCA GACTGAGGAA GGCACAGGGC CCCAGGGCGA GCCAGTGGTG
    GCCGGGGCCC TCACCCAGGA CCACATCCAG GCAGAAGCAG GGGATGGGCC CCCAGAGCCT
    CCTGTGGAGC TCAAGCCCCG AGACATAAAG CGTATCAGCC TGCGTTTCAG GAAGAGGCGG
    AGGGAGACCA CAGAGCCTGT ACAACCCACA GCCACTGGTG TGGTGGTGGC CTCCCGCAGA
    GAGAAGTGGA GTCGCCCCCG GGAAAGAATG ACAGCCATGG GGCTCCGACT GCAGACCTTC
    TGCCTGGCCG TGGCCCAGAG CATGTACCGG CCCCTGCGGC GCTTCTTCGA TGACATCTTG
    CACACCAAGT ACCGTGCAGC CACCGATGTC TACGCGCTCA TGTTTCTGGC TGATGTGGTC
    GACTTCATCA TCATCATCTT CGGCTTCTGG GCCTTTGGGA AACACTCGGC AGCCACAGAC
    ATCACATCCT CGCTGTCGGA CGACCAGGTG CCGGAGGCCT TCCTGGTCAT GCTGCTCATC
    CAGTTCAGCA CCATGGTCAT TGACCGTGCC CTCTACCTCC GCAAGACCGT GCTGGGCAAG
    CTGGCCTTCC AGGTTGTCCT GGTGCTGGCC ATCCACCTCT GGATGTTCTT TATTCTGCCC
    GCTGTCACCG AGAGGATGTT CAACCAGAAC GCGGTGGCTC AGCTGTGGTA CTTCGTGAAG
    TGCATCTACT TCTCCCTGTC TGCCTACCAG ATCCGCTGTG GCTATCCGAC CCGTATCCTT
    GGCAACTTCC TCACCAAGAA GTACAATCAC CTGAACCTCT TCCTCTTCCA GGGGTTCCGG
    CTTGTGCCAT TCCTGGTGGA GCTGCGGGCC GTGATGGACT GGGTATGGAC AGACACCACG
    CTGTCCCTGT CCAGCTGGAT GTGTGTGGAG GACATCTATG CCAACATCTT CATCATCAAG
    TGCAGCCGTG AGACTGAGAA GAAATATCCC CAGCCGAAGG GGCAGAAGAA GAAGAAGATT
    GTCAAGTACG GCATGGGCGG TCTCATCATC CTGTTCCTCG TGGCTATCAT CTGGTTCCCG
    TTGCTTTTCA TGTCCCTGGT ACGCTCTGTG GTTGGTGTCG TCAACCAGCC CATCGATGTT
    ACTGTCACCC TTAAGCTGGG TGGCTATGAG CCCCTCTTTA CCATGAGTGC CCAGCAGCCG
    TCCATCGTGC CGTTCACACA GCAGGCCTAT GAGGAGCTGT CCAGACAGTT TGACCCTAAC
    CCGTTGGCCA TGCAGTTCAT CAGCCAGTAC AGCCCCGAGG ACATCGTCAC GGCACAGATC
    GAGGGCAGTT CAGGGGCACT GTGGCGCATC AGCCCACCCA GCCGGGCCCA GATGAAGCGG
    GAGCTATACA ATGGTACAGC TGACATCACC CTGCGCTTCA CCTGGAACTT CCAGAGGGAC
    CTGGCCAAGG GCGGCACCGT GGAGTACACC AATGAGAAGC ACACCCTGGA CTGGGCCCCA
    AATAGCACTG AGCGGCGGCA GCTGGCCAGC CTGCTTGAGG GCACCTCGGA CCAGTCCGTG
    GTCATCCCCC ACCTCTTTCC CAAGTACATC CGTGCCCCTA ATGGGCCTGA AGCCAACCCA
    GTGAAGCAGC TGCAGCCCAA CGAGGAGGCC GACTACCTAG GCGTGCGCAT CCAGCTGCGG
    AGGGAGCGCG TGGGCTCAGG GGCCGCTGGC TTCCTTGAGT GGTGGGTCAT CGAGCTGCAG
    GATTGCCAGG CCGAGTGCAA CCTGCTGCCC ATGGTCATCT TCAGTGACAA GGTCAGCCCA
    CCCAGCCTTG GCTTCCTGGC TGGCTACGGG ATCATGGGGC TCTATGTGTC CATCGTGCTG
    GTTATCGGCA AGTTTGTACG CGGCTTCTTC AGTGAGATCT CCCACTCCAT CATGTTTGAG
    GAGCTGCCGT GTGTGGACCG CATCCTCAAG CTCTGCCAGG ACATCTTCCT GGTGCGGGAG
    ACGCGGGAGC TGGAGCTGGA AGAGGAGCTT TATGCCAAGC TTATCTTCCT GTACCGCTCG
    CCTGAGACGA TGATCAAGTG GACCCGTGAG AAGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288457
    Clone ID Related Accession (Same CDS sequence) XM_022419090.1
    Accession Version XM_022419090.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product piezo-type mechanosensitive ion channel component 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    481
    541
    601
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGCTGCCCA CACAGGTCTT CCTGTTGTCC CCTCCTCTTA GCTTCCCTGC CACACCCAAG 
    CTCCTGATCT GGGTGAGGGC CTCGGGCCCC AGGACAAAGC CAGGATGGAG GACAGGCCAA
    GCCTGCCTGT TCCGCTATAA CGCGCTGTCT CTGGTCTATC TGCTCTTCCT GCTGCTGCTG
    CCCTGGTTCC CGGGCCCCTG TCGGCGCGGC TTCCCAGGCC ACACTCGTCG CCTGCTCCGG
    GCCCTGCTGG TTTTCAGCCT CATCTTCCTG GCAGCTCACC TGACCTTCCA GATATGCCTG
    CACACTGTGC CCCACCTGTA CCAGCTTCTG GAGCCCAGCT GTGGCCCCTG GGAGACCCTC
    TCTCGGCACA TTGGGGTCAC AAGGCTGGAC CTGAAGGACA TCCCCACTGC TGTCCGGCTG
    GTGGCCCCCG ACCTTGGTGT CCTGGTGGTC TGCGCTGTGT GCCTCGGCCT CTGCGGGCGC
    CTCACACAGG AAGCCCCACG GAGCCAGCGT GCCCAGGAGC TGGACGATGA TGATGATGGG
    GAAGTGGACA CCGGCTCCCC TGTGGGGCTT CAGGGAGCCC CCGTGCAGGC CCCCACGCGG
    AGGTCACGGT TGGCCACCCG GTTCCAGATC ACAGCCCACT GGCTGTTGGT GGCCGCGGGA
    AGGACCCTGG CTATTGTGCT GCTCGCATTG GCAGGCATCG CCCACCCCTC TGCCTTCTCC
    AGCGTCTACT TCCTAGTCTT CCTGGCCACC TGCACCTGGT GGGCATGCCA CTTTCCCATC
    AGCCTCCTGG GCTTCAGCAC GCTCTGCGTC ACAGTGGGCT GCTTCGGTGC CGGCCATCTC
    CTCTGCATCT ACTGCTACCA GACGCCCTTT GCCCAGACCG TGCTCCCTCC TGCTGGAATC
    TGGGCCAGGG TGTTTGGTCT CAAGGCCTTG GTGACCCACG GCAACTGCTC CAGCCCCAAC
    GTGCTGGTCT TCAGTACCAG CCACGACTGG CCCATCTACG TGAGCCCCGG CATCTTGCTG
    CTTCTCTACT ACACGGTGAC CTCACTCCTG AAGCTCCGCA CTCGCCAGCC GTTGGACCAG
    AGGAAGGAAG TGGCCAGGAA CGACGAGGAG CAGGAGGTAG AGCTGACCCA GGTGGACCAG
    TGGCTCCAGG ACCAGGCCAG AGCTGCAGCC ACCAAGGAGG AGGGGGCTGC CCAGCACATG
    CTGCCCACAA CCGTGGGGTC TGACTGGGAG ACCGACAGCT GTACGGTGCA TGACCTGACT
    GGCCACACCC CCGTCCGGCA GCGTCCAGTG CACCCCAGGC TGGCTGAGCC TCAAGAGACA
    TCTACCCTCC ACAGTCTGGG TCATCTCATT ATGGACCAGA GCTACGTGTG TGCCCTCATT
    GCCATGATGG TATGGAGCAT CACCTACCAT AGTTGGCTGA CCTTCGTGCT GCTGCTCTGG
    GCCTGCCTCA TCTGGACGGT GCGCAGCCGC CACCAGATGG CCATGCTCTG CTCACCCTTC
    ATCCTGCTCT ACGGGCTGGC TCTGTGTGGC CTGCGCTATG TGTGGGCCAT GGACCTGCGT
    CCCGAGCTGC CTACCACCCT GGGCCCCGTC AGCCTGCGTC AGCTGGGGCT GGAGCATACC
    CGCTACCCTT GCCTGGACCT TGGCGCCATG TTGCTCTACA CCCTGACCTT CTGGCTGCTG
    CTGCGCCGGT TTGTCAAGGA GAAGCTGCTG AAGAAGGGGA AGGTACCTGC TGCCCTGACA
    GAGGTCACTG TGGCTGACAC AGAGCCAACA CGGGCACGGA CGCTGCTGCA GAGCCTGGGG
    GAGCTGGTCA CAGGCCTGTA CGCCAAGTAC TGGATCTACG TGTGTGCCGG CATGTTCATT
    GTGGTCAGCT TCGCCGGCCG CCTCGTCGTC TACAAGATCG TGTACATGCT CCTTTTCCTG
    CTCTGCCTCA CCCTCTTCCA GGTCTACTAC AGCCTGTGGA GGAAGCTGCT CAAGGTGTTC
    TGGTGGCTGG TGGTGGCCTA TACCATGCTG GTGCTCATCG CCGTCTACAC TTTCCAGTTC
    CAGGACTTCC CCATGTACTG GCGCAACCTG ACGGGCCTCA CTGACGAACA GCTGGGGGAC
    CTGGGCCTGG AGCAGTTCAG CGTGTCGGAG CTTTTCTCCA GCATCCTGAT CCCGGGCTTC
    TTCCTGTTGG CCTGCATCCT GCAGCTGCAC TATTTCCACC GGCCGTTCAT GCAGCTCACG
    GACCTGGAGC ATGTGCCCCC GCCTGGTGCC TGCCCCCCAC GGTGGGCCCA CAGGCAGGAT
    GTGGTCAGTG GGACCCCGCT GCTGCAGGAG GAGGAGGACG GGCTGAGCAC CGCCTGCCCC
    CACCAGGCCA TGCAGGTCAC AGAAGCCAAC AAGTGGGGCC TGGTGGCGGA ACGGCTGCTG
    GACCTGGCTT CCGGCTTCTC GGACGTCCTC ACCCGTGTGC AGGTGCTGCT GCGGCGCCTT
    CTAGAGTTGC ACATCCTCAA GCTGGTGGCC CTGTACACTG TGTGGGTGGC CCTGAAGGAG
    GTGTCCGTGA TGAACCTGCT GCTGGTCGTG CTGTGGGCCT TCGCGCTGCC CTACCCCCGC
    TTCCGGCCCA TGGCCTCCTG CCTGTCCACT GTGTGGACCT GCATCATCAT CGTGTGCAAG
    ATGCTGTACC AGCTCAAGGT CGTAAGCCCG CACGAGTACT CCAGCAACTG CACCGAGCCC
    CTCCCCAACA GCACCAACCT GCAGAAGATG GAGATCAAGC AGTCCTTGCT ATACCGTGGG
    CCCGTTGACC CTGCCAACTG GTTTGGGGTG CGGAAGGGCT TCCCAAACCT AGGCTACATC
    CAGAACCACC TGCAGGTCCT GTTGCTGCTG GTGTTCGAGG CCATCGTCTA CCGGCGTCAG
    GAGCACCACC GTCGGCAGCA CCAGCTGGCC CCACTGCCCG CCCAGGCCGT CTGTGCCGAC
    GGCACCCGCC AGCGGCTGGA CCAGGACCTG CTCAGCTGCC TCAAGTACTT TGTCAACTTC
    TTTTTCTACA AATTTGGGCT AGAGATATGC TTCCTGATGG CCGTGAACGT GATTGGGCAG
    CGCATGAACT TCATGGTCAT CCTGCACGGC TGCTGGCTGG TGGCCATCCT CACACGGCGG
    CGCCGTGCAG CCATCGCCCG CCTCTGGCCC AACTACTGTC TCTTCCTGTC CCTGTTCTTG
    CTGTACCAGT ACCTGCTGTG CCTGGGCATC CCCCCGGCCC TGTGCATCGA CTACCCGTGG
    CGCTGGAGCC AGGCCATCCC CATGAACTCA GCACTCATCA AGTGGCTGTA CCTGCCCGAC
    TTCTTCAGAA CCCCCAATTC CACCAACCTC ATCAGCGACT TCCTGCTCCT GCTCTGCGCC
    TCCCAGCAGT GGCAGGTGTT CTCGGCTGAG CGCACGGAGG AATGGCAGCA CATGGCCGGC
    GTCAACACTG ACCGCCTGGA GCTGCCATTG GGTGAGCCCA ACGCCGTGCC CAACTTCATC
    TACTGCAGGT CCTACCTCGA CATGCTGAAG GTGGCTGTCT TCCGCTACCT CTTCTGGCTG
    GTGCTGGTAG TGGTATTTGT CACGGGAGCC ACCCGCGTCA GCGTCTTCGG GCTGGGCTAC
    CTGTTGGCCT GTTTCTATCT GCTGCTCTTC GGCACCAGCC TATTACAGAA GCACACACGC
    ACCCGCCTCG TGCTGTGGGA CTGCCTCATT CTCTACAACG TCACTGTCAT CATCTCTAAG
    AACATGCTCT CGCTCCTGTC CTGCGTTTTT GTGGAGCAAA TGCAGAGCAA CTTCTGCTGG
    GTTATCCAGC TTTTCAGCCT CGTGTGCACC GTCAAAGGCT ACTATGATCC CAAGGAGATG
    CTGAGCAGGG ACCGGGATTG CCTGCTGCCC GTGGAAGAGG CCGGTGTGCT CTGGGACAGC
    ATCTGCTTCC TGTTCCTTCT GCTGCAGCGG CGCGTCTTCC TCAGCCGCTA CTTCCTGCAT
    GTCAGCGCCG AGCTCCAGGC CACCGCCTTG CAGGCCTCCA GGGGCTTCGC GCTGTACAAT
    GCTGCCAACT TCAAAAGCAT CGATCTCCAC CGTAAGGCCG AGGAGAAGTC CCTGGCCCAG
    CTGAAAAGAC AGATGGAGCG CATCCGTGCC AAGCAAGAGA AGCACAGGCA GAGCCGAGCG
    GGCCGCAGCC GACCCCAGGA CACCCCAGAC CCCACCCAGG AGCCAGGGCC TGGCAGTCCA
    GGGGGCTCCT CCCTGCCACG GAGACAGTGG TGGCGGCCCT GGCTGGACCA CGCCACAGTC
    ATCCACTCTG GGGACTACTT CCTATTTGAG TCGGATAGTG AGGAGGAGGA GGAGGCCCTG
    CCCGAGGACC CCAGGCCGTC AGCACAGAGT GCCTTTCAGA TGGCGTACCA GGCATGGGTG
    ACCAATGCGC AGACAGTGCT GAGGCAGCAG CGGGAGGAGC AGGCGAGGCA GGACCAGACA
    GGGCAGTTGC CCACGGGAGG CGGCCCGGGA CAGGAGGCAG AGCCAGCGGA TGGCCCAGAC
    GAGGTGGTGG CTGGCCGCAG CCACGTGATG CAGCGAGTGC TGAGCACTGT GCAGTTTCTG
    TGGGTGCTGG GCCAAGCGCT GGTAGATGGG CTGACAGACT GGCTGCACAC CTTCACACGG
    CACCACCGCG CCATGAGTGA CGTGCTGCGC GCCGAGCGCT ACCTGTACAC ACAGGCGCTG
    GTTGAGGGCA GAGAGGTGCA CCGGAGCTTG CTGGACCAGC TCTACACTGG TGAAGCCGAG
    GCTGCCCCCG CAGTCCCCTT GGGGGTCCGA GATGCACCAA GTACAGCATC AAGCAGCAGC
    AGCGAGGAGG TGGCCACTGA GCCTGGGGCT TCCCTACACG GCTCTCGGGA GCTTCCTGCT
    GGCACTCCTA CCCGGATGCG CACAGCCAGT GAGCTGCTCC TCAGCAGGCA CCTCCGCATC
    CAGGAGCTGG AGGAGGCCGA GCTGTTTGAA GCAGGGCAGG GCCGGGCACT GCGGCTGCTG
    CAGGCCGCAT ACCAGTGTGT GGCTGCCCAC TCAGAACTGC TCTGCTACTT CATCATCATC
    CTCAACCACA TGGTCACTGC CTCAGCCACA TCCCTGGTGC TGCCTGTGCT CGTCTTCCTG
    TGGGCCATGC TGTCCATCCC CAGGCCCAGC AAGCGCTTCT GGATGACGGC CATCATCTTC
    ACCGAGGTGT CTGTGGTCAC CAAGTACCTG TTCCAGTTTG GCTTCTTCCC CTGGAACACA
    CACACAGTGC TCCGGCGCTA CGAGAACAAG CCGTACTTCC CACCGCGCAT CCTGGGTGTG
    GAGAAGACAG ACAGCTACAT AAAGTATGAC CTGGTGCAGC TCATGGCCCT CTTCTTCCAC
    CGCTCCCAGC TGCTGTGCTA TGGTCTCTGG GATCATGAGG AGGACCCGCT GTCCAAGGAG
    CATGACCGTG GAAGTGAGAA GAAAGGGACC GAGGAGGAAC AGGCACCACT GGAACCCCAG
    ACTGAGGAAG GCACAGGGCC CCAGGGCGAG CCAGTGGTGG CCGGGGCCCT CACCCAGGAC
    CACATCCAGG CAGAAGCAGG GGATGGGCCC CCAGAGCCTC CTGTGGAGCT CAAGCCCCGA
    GACATAAAGC GTATCAGCCT GCGTTTCAGG AAGAGGCGGA GGGAGACCAC AGAGCCTGTA
    CAACCCACAG CCACTGAAGG TGTGGTGGTG GCCTCCCGCA GAGAGAAGTG GAGTCGCCCC
    CGGGAAAGAA TGACAGCCAT GGGGCTCCGA CTGCAGACCT TCTGCCTGGC CGTGGCCCAG
    AGCATGTACC GGCCCCTGCG GCGCTTCTTC GATGACATCT TGCACACCAA GTACCGTGCA
    GCCACCGATG TCTACGCGCT CATGTTTCTG GCTGATGTGG TCGACTTCAT CATCATCATC
    TTCGGCTTCT GGGCCTTTGG GAAACACTCG GCAGCCACAG ACATCACATC CTCGCTGTCG
    GACGACCAGG TGCCGGAGGC CTTCCTGGTC ATGCTGCTCA TCCAGTTCAG CACCATGGTC
    ATTGACCGTG CCCTCTACCT CCGCAAGACC GTGCTGGGCA AGCTGGCCTT CCAGGTTGTC
    CTGGTGCTGG CCATCCACCT CTGGATGTTC TTTATTCTGC CCGCTGTCAC CGAGAGGATG
    TTCAACCAGA ACGCGGTGGC TCAGCTGTGG TACTTCGTGA AGTGCATCTA CTTCTCCCTG
    TCTGCCTACC AGATCCGCTG TGGCTATCCG ACCCGTATCC TTGGCAACTT CCTCACCAAG
    AAGTACAATC ACCTGAACCT CTTCCTCTTC CAGGGGTTCC GGCTTGTGCC ATTCCTGGTG
    GAGCTGCGGG CCGTGATGGA CTGGGTATGG ACAGACACCA CGCTGTCCCT GTCCAGCTGG
    ATGTGTGTGG AGGACATCTA TGCCAACATC TTCATCATCA AGTGCAGCCG TGAGACTGAG
    AAGAAATATC CCCAGCCGAA GGGGCAGAAG AAGAAGAAGA TTGTCAAGTA CGGCATGGGC
    GGTCTCATCA TCCTGTTCCT CGTGGCTATC ATCTGGTTCC CGTTGCTTTT CATGTCCCTG
    GTACGCTCTG TGGTTGGTGT CGTCAACCAG CCCATCGATG TTACTGTCAC CCTTAAGCTG
    GGTGGCTATG AGCCCCTCTT TACCATGAGT GCCCAGCAGC CGTCCATCGT GCCGTTCACA
    CAGCAGGCCT ATGAGGAGCT GTCCAGACAG TTTGACCCTA ACCCGTTGGC CATGCAGTTC
    ATCAGCCAGT ACAGCCCCGA GGACATCGTC ACGGCACAGA TCGAGGGCAG TTCAGGGGCA
    CTGTGGCGCA TCAGCCCACC CAGCCGGGCC CAGATGAAGC GGGAGCTATA CAATGGTACA
    GCTGACATCA CCCTGCGCTT CACCTGGAAC TTCCAGAGGG ACCTGGCCAA GGGCGGCACC
    GTGGAGTACA CCAATGAGAA GCACACCCTG GACTGGGCCC CAAATAGCAC TGAGCGGCGG
    CAGCTGGCCA GCCTGCTTGA GGGCACCTCG GACCAGTCCG TGGTCATCCC CCACCTCTTT
    CCCAAGTACA TCCGTGCCCC TAATGGGCCT GAAGCCAACC CAGTGAAGCA GCTGCAGCCC
    AACGAGGAGG CCGACTACCT AGGCGTGCGC ATCCAGCTGC GGAGGGAGCG CGTGGGCTCA
    GGGGCCGCTG GCTTCCTTGA GTGGTGGGTC ATCGAGCTGC AGGATTGCCA GGCCGAGTGC
    AACCTGCTGC CCATGGTCAT CTTCAGTGAC AAGGTCAGCC CACCCAGCCT TGGCTTCCTG
    GCTGGCTACG GGATCATGGG GCTCTATGTG TCCATCGTGC TGGTTATCGG CAAGTTTGTA
    CGCGGCTTCT TCAGTGAGAT CTCCCACTCC ATCATGTTTG AGGAGCTGCC GTGTGTGGAC
    CGCATCCTCA AGCTCTGCCA GGACATCTTC CTGGTGCGGG AGACGCGGGA GCTGGAGCTG
    GAAGAGGAGC TTTATGCCAA GCTTATCTTC CTGTACCGCT CGCCTGAGAC GATGATCAAG
    TGGACCCGTG AGAAGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274798.1
    CDS23..7543
    Translation

    Target ORF information:

    RefSeq Version XM_022419090.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022419090.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGCTGCCCA CACAGGTCTT CCTGTTGTCC CCTCCTCTTA GCTTCCCTGC CACACCCAAG 
    CTCCTGATCT GGGTGAGGGC CTCGGGCCCC AGGACAAAGC CAGGATGGAG GACAGGCCAA
    GCCTGCCTGT TCCGCTATAA CGCGCTGTCT CTGGTCTATC TGCTCTTCCT GCTGCTGCTG
    CCCTGGTTCC CGGGCCCCTG TCGGCGCGGC TTCCCAGGCC ACACTCGTCG CCTGCTCCGG
    GCCCTGCTGG TTTTCAGCCT CATCTTCCTG GCAGCTCACC TGACCTTCCA GATATGCCTG
    CACACTGTGC CCCACCTGTA CCAGCTTCTG GAGCCCAGCT GTGGCCCCTG GGAGACCCTC
    TCTCGGCACA TTGGGGTCAC AAGGCTGGAC CTGAAGGACA TCCCCACTGC TGTCCGGCTG
    GTGGCCCCCG ACCTTGGTGT CCTGGTGGTC TGCGCTGTGT GCCTCGGCCT CTGCGGGCGC
    CTCACACAGG AAGCCCCACG GAGCCAGCGT GCCCAGGAGC TGGACGATGA TGATGATGGG
    GAAGTGGACA CCGGCTCCCC TGTGGGGCTT CAGGGAGCCC CCGTGCAGGC CCCCACGCGG
    AGGTCACGGT TGGCCACCCG GTTCCAGATC ACAGCCCACT GGCTGTTGGT GGCCGCGGGA
    AGGACCCTGG CTATTGTGCT GCTCGCATTG GCAGGCATCG CCCACCCCTC TGCCTTCTCC
    AGCGTCTACT TCCTAGTCTT CCTGGCCACC TGCACCTGGT GGGCATGCCA CTTTCCCATC
    AGCCTCCTGG GCTTCAGCAC GCTCTGCGTC ACAGTGGGCT GCTTCGGTGC CGGCCATCTC
    CTCTGCATCT ACTGCTACCA GACGCCCTTT GCCCAGACCG TGCTCCCTCC TGCTGGAATC
    TGGGCCAGGG TGTTTGGTCT CAAGGCCTTG GTGACCCACG GCAACTGCTC CAGCCCCAAC
    GTGCTGGTCT TCAGTACCAG CCACGACTGG CCCATCTACG TGAGCCCCGG CATCTTGCTG
    CTTCTCTACT ACACGGTGAC CTCACTCCTG AAGCTCCGCA CTCGCCAGCC GTTGGACCAG
    AGGAAGGAAG TGGCCAGGAA CGACGAGGAG CAGGAGGTAG AGCTGACCCA GGTGGACCAG
    TGGCTCCAGG ACCAGGCCAG AGCTGCAGCC ACCAAGGAGG AGGGGGCTGC CCAGCACATG
    CTGCCCACAA CCGTGGGGTC TGACTGGGAG ACCGACAGCT GTACGGTGCA TGACCTGACT
    GGCCACACCC CCGTCCGGCA GCGTCCAGTG CACCCCAGGC TGGCTGAGCC TCAAGAGACA
    TCTACCCTCC ACAGTCTGGG TCATCTCATT ATGGACCAGA GCTACGTGTG TGCCCTCATT
    GCCATGATGG TATGGAGCAT CACCTACCAT AGTTGGCTGA CCTTCGTGCT GCTGCTCTGG
    GCCTGCCTCA TCTGGACGGT GCGCAGCCGC CACCAGATGG CCATGCTCTG CTCACCCTTC
    ATCCTGCTCT ACGGGCTGGC TCTGTGTGGC CTGCGCTATG TGTGGGCCAT GGACCTGCGT
    CCCGAGCTGC CTACCACCCT GGGCCCCGTC AGCCTGCGTC AGCTGGGGCT GGAGCATACC
    CGCTACCCTT GCCTGGACCT TGGCGCCATG TTGCTCTACA CCCTGACCTT CTGGCTGCTG
    CTGCGCCGGT TTGTCAAGGA GAAGCTGCTG AAGAAGGGGA AGGTACCTGC TGCCCTGACA
    GAGGTCACTG TGGCTGACAC AGAGCCAACA CGGGCACGGA CGCTGCTGCA GAGCCTGGGG
    GAGCTGGTCA CAGGCCTGTA CGCCAAGTAC TGGATCTACG TGTGTGCCGG CATGTTCATT
    GTGGTCAGCT TCGCCGGCCG CCTCGTCGTC TACAAGATCG TGTACATGCT CCTTTTCCTG
    CTCTGCCTCA CCCTCTTCCA GGTCTACTAC AGCCTGTGGA GGAAGCTGCT CAAGGTGTTC
    TGGTGGCTGG TGGTGGCCTA TACCATGCTG GTGCTCATCG CCGTCTACAC TTTCCAGTTC
    CAGGACTTCC CCATGTACTG GCGCAACCTG ACGGGCCTCA CTGACGAACA GCTGGGGGAC
    CTGGGCCTGG AGCAGTTCAG CGTGTCGGAG CTTTTCTCCA GCATCCTGAT CCCGGGCTTC
    TTCCTGTTGG CCTGCATCCT GCAGCTGCAC TATTTCCACC GGCCGTTCAT GCAGCTCACG
    GACCTGGAGC ATGTGCCCCC GCCTGGTGCC TGCCCCCCAC GGTGGGCCCA CAGGCAGGAT
    GTGGTCAGTG GGACCCCGCT GCTGCAGGAG GAGGAGGACG GGCTGAGCAC CGCCTGCCCC
    CACCAGGCCA TGCAGGTCAC AGAAGCCAAC AAGTGGGGCC TGGTGGCGGA ACGGCTGCTG
    GACCTGGCTT CCGGCTTCTC GGACGTCCTC ACCCGTGTGC AGGTGCTGCT GCGGCGCCTT
    CTAGAGTTGC ACATCCTCAA GCTGGTGGCC CTGTACACTG TGTGGGTGGC CCTGAAGGAG
    GTGTCCGTGA TGAACCTGCT GCTGGTCGTG CTGTGGGCCT TCGCGCTGCC CTACCCCCGC
    TTCCGGCCCA TGGCCTCCTG CCTGTCCACT GTGTGGACCT GCATCATCAT CGTGTGCAAG
    ATGCTGTACC AGCTCAAGGT CGTAAGCCCG CACGAGTACT CCAGCAACTG CACCGAGCCC
    CTCCCCAACA GCACCAACCT GCAGAAGATG GAGATCAAGC AGTCCTTGCT ATACCGTGGG
    CCCGTTGACC CTGCCAACTG GTTTGGGGTG CGGAAGGGCT TCCCAAACCT AGGCTACATC
    CAGAACCACC TGCAGGTCCT GTTGCTGCTG GTGTTCGAGG CCATCGTCTA CCGGCGTCAG
    GAGCACCACC GTCGGCAGCA CCAGCTGGCC CCACTGCCCG CCCAGGCCGT CTGTGCCGAC
    GGCACCCGCC AGCGGCTGGA CCAGGACCTG CTCAGCTGCC TCAAGTACTT TGTCAACTTC
    TTTTTCTACA AATTTGGGCT AGAGATATGC TTCCTGATGG CCGTGAACGT GATTGGGCAG
    CGCATGAACT TCATGGTCAT CCTGCACGGC TGCTGGCTGG TGGCCATCCT CACACGGCGG
    CGCCGTGCAG CCATCGCCCG CCTCTGGCCC AACTACTGTC TCTTCCTGTC CCTGTTCTTG
    CTGTACCAGT ACCTGCTGTG CCTGGGCATC CCCCCGGCCC TGTGCATCGA CTACCCGTGG
    CGCTGGAGCC AGGCCATCCC CATGAACTCA GCACTCATCA AGTGGCTGTA CCTGCCCGAC
    TTCTTCAGAA CCCCCAATTC CACCAACCTC ATCAGCGACT TCCTGCTCCT GCTCTGCGCC
    TCCCAGCAGT GGCAGGTGTT CTCGGCTGAG CGCACGGAGG AATGGCAGCA CATGGCCGGC
    GTCAACACTG ACCGCCTGGA GCTGCCATTG GGTGAGCCCA ACGCCGTGCC CAACTTCATC
    TACTGCAGGT CCTACCTCGA CATGCTGAAG GTGGCTGTCT TCCGCTACCT CTTCTGGCTG
    GTGCTGGTAG TGGTATTTGT CACGGGAGCC ACCCGCGTCA GCGTCTTCGG GCTGGGCTAC
    CTGTTGGCCT GTTTCTATCT GCTGCTCTTC GGCACCAGCC TATTACAGAA GCACACACGC
    ACCCGCCTCG TGCTGTGGGA CTGCCTCATT CTCTACAACG TCACTGTCAT CATCTCTAAG
    AACATGCTCT CGCTCCTGTC CTGCGTTTTT GTGGAGCAAA TGCAGAGCAA CTTCTGCTGG
    GTTATCCAGC TTTTCAGCCT CGTGTGCACC GTCAAAGGCT ACTATGATCC CAAGGAGATG
    CTGAGCAGGG ACCGGGATTG CCTGCTGCCC GTGGAAGAGG CCGGTGTGCT CTGGGACAGC
    ATCTGCTTCC TGTTCCTTCT GCTGCAGCGG CGCGTCTTCC TCAGCCGCTA CTTCCTGCAT
    GTCAGCGCCG AGCTCCAGGC CACCGCCTTG CAGGCCTCCA GGGGCTTCGC GCTGTACAAT
    GCTGCCAACT TCAAAAGCAT CGATCTCCAC CGTAAGGCCG AGGAGAAGTC CCTGGCCCAG
    CTGAAAAGAC AGATGGAGCG CATCCGTGCC AAGCAAGAGA AGCACAGGCA GAGCCGAGCG
    GGCCGCAGCC GACCCCAGGA CACCCCAGAC CCCACCCAGG AGCCAGGGCC TGGCAGTCCA
    GGGGGCTCCT CCCTGCCACG GAGACAGTGG TGGCGGCCCT GGCTGGACCA CGCCACAGTC
    ATCCACTCTG GGGACTACTT CCTATTTGAG TCGGATAGTG AGGAGGAGGA GGAGGCCCTG
    CCCGAGGACC CCAGGCCGTC AGCACAGAGT GCCTTTCAGA TGGCGTACCA GGCATGGGTG
    ACCAATGCGC AGACAGTGCT GAGGCAGCAG CGGGAGGAGC AGGCGAGGCA GGACCAGACA
    GGGCAGTTGC CCACGGGAGG CGGCCCGGGA CAGGAGGCAG AGCCAGCGGA TGGCCCAGAC
    GAGGTGGTGG CTGGCCGCAG CCACGTGATG CAGCGAGTGC TGAGCACTGT GCAGTTTCTG
    TGGGTGCTGG GCCAAGCGCT GGTAGATGGG CTGACAGACT GGCTGCACAC CTTCACACGG
    CACCACCGCG CCATGAGTGA CGTGCTGCGC GCCGAGCGCT ACCTGTACAC ACAGGCGCTG
    GTTGAGGGCA GAGAGGTGCA CCGGAGCTTG CTGGACCAGC TCTACACTGG TGAAGCCGAG
    GCTGCCCCCG CAGTCCCCTT GGGGGTCCGA GATGCACCAA GTACAGCATC AAGCAGCAGC
    AGCGAGGAGG TGGCCACTGA GCCTGGGGCT TCCCTACACG GCTCTCGGGA GCTTCCTGCT
    GGCACTCCTA CCCGGATGCG CACAGCCAGT GAGCTGCTCC TCAGCAGGCA CCTCCGCATC
    CAGGAGCTGG AGGAGGCCGA GCTGTTTGAA GCAGGGCAGG GCCGGGCACT GCGGCTGCTG
    CAGGCCGCAT ACCAGTGTGT GGCTGCCCAC TCAGAACTGC TCTGCTACTT CATCATCATC
    CTCAACCACA TGGTCACTGC CTCAGCCACA TCCCTGGTGC TGCCTGTGCT CGTCTTCCTG
    TGGGCCATGC TGTCCATCCC CAGGCCCAGC AAGCGCTTCT GGATGACGGC CATCATCTTC
    ACCGAGGTGT CTGTGGTCAC CAAGTACCTG TTCCAGTTTG GCTTCTTCCC CTGGAACACA
    CACACAGTGC TCCGGCGCTA CGAGAACAAG CCGTACTTCC CACCGCGCAT CCTGGGTGTG
    GAGAAGACAG ACAGCTACAT AAAGTATGAC CTGGTGCAGC TCATGGCCCT CTTCTTCCAC
    CGCTCCCAGC TGCTGTGCTA TGGTCTCTGG GATCATGAGG AGGACCCGCT GTCCAAGGAG
    CATGACCGTG GAAGTGAGAA GAAAGGGACC GAGGAGGAAC AGGCACCACT GGAACCCCAG
    ACTGAGGAAG GCACAGGGCC CCAGGGCGAG CCAGTGGTGG CCGGGGCCCT CACCCAGGAC
    CACATCCAGG CAGAAGCAGG GGATGGGCCC CCAGAGCCTC CTGTGGAGCT CAAGCCCCGA
    GACATAAAGC GTATCAGCCT GCGTTTCAGG AAGAGGCGGA GGGAGACCAC AGAGCCTGTA
    CAACCCACAG CCACTGAAGG TGTGGTGGTG GCCTCCCGCA GAGAGAAGTG GAGTCGCCCC
    CGGGAAAGAA TGACAGCCAT GGGGCTCCGA CTGCAGACCT TCTGCCTGGC CGTGGCCCAG
    AGCATGTACC GGCCCCTGCG GCGCTTCTTC GATGACATCT TGCACACCAA GTACCGTGCA
    GCCACCGATG TCTACGCGCT CATGTTTCTG GCTGATGTGG TCGACTTCAT CATCATCATC
    TTCGGCTTCT GGGCCTTTGG GAAACACTCG GCAGCCACAG ACATCACATC CTCGCTGTCG
    GACGACCAGG TGCCGGAGGC CTTCCTGGTC ATGCTGCTCA TCCAGTTCAG CACCATGGTC
    ATTGACCGTG CCCTCTACCT CCGCAAGACC GTGCTGGGCA AGCTGGCCTT CCAGGTTGTC
    CTGGTGCTGG CCATCCACCT CTGGATGTTC TTTATTCTGC CCGCTGTCAC CGAGAGGATG
    TTCAACCAGA ACGCGGTGGC TCAGCTGTGG TACTTCGTGA AGTGCATCTA CTTCTCCCTG
    TCTGCCTACC AGATCCGCTG TGGCTATCCG ACCCGTATCC TTGGCAACTT CCTCACCAAG
    AAGTACAATC ACCTGAACCT CTTCCTCTTC CAGGGGTTCC GGCTTGTGCC ATTCCTGGTG
    GAGCTGCGGG CCGTGATGGA CTGGGTATGG ACAGACACCA CGCTGTCCCT GTCCAGCTGG
    ATGTGTGTGG AGGACATCTA TGCCAACATC TTCATCATCA AGTGCAGCCG TGAGACTGAG
    AAGAAATATC CCCAGCCGAA GGGGCAGAAG AAGAAGAAGA TTGTCAAGTA CGGCATGGGC
    GGTCTCATCA TCCTGTTCCT CGTGGCTATC ATCTGGTTCC CGTTGCTTTT CATGTCCCTG
    GTACGCTCTG TGGTTGGTGT CGTCAACCAG CCCATCGATG TTACTGTCAC CCTTAAGCTG
    GGTGGCTATG AGCCCCTCTT TACCATGAGT GCCCAGCAGC CGTCCATCGT GCCGTTCACA
    CAGCAGGCCT ATGAGGAGCT GTCCAGACAG TTTGACCCTA ACCCGTTGGC CATGCAGTTC
    ATCAGCCAGT ACAGCCCCGA GGACATCGTC ACGGCACAGA TCGAGGGCAG TTCAGGGGCA
    CTGTGGCGCA TCAGCCCACC CAGCCGGGCC CAGATGAAGC GGGAGCTATA CAATGGTACA
    GCTGACATCA CCCTGCGCTT CACCTGGAAC TTCCAGAGGG ACCTGGCCAA GGGCGGCACC
    GTGGAGTACA CCAATGAGAA GCACACCCTG GACTGGGCCC CAAATAGCAC TGAGCGGCGG
    CAGCTGGCCA GCCTGCTTGA GGGCACCTCG GACCAGTCCG TGGTCATCCC CCACCTCTTT
    CCCAAGTACA TCCGTGCCCC TAATGGGCCT GAAGCCAACC CAGTGAAGCA GCTGCAGCCC
    AACGAGGAGG CCGACTACCT AGGCGTGCGC ATCCAGCTGC GGAGGGAGCG CGTGGGCTCA
    GGGGCCGCTG GCTTCCTTGA GTGGTGGGTC ATCGAGCTGC AGGATTGCCA GGCCGAGTGC
    AACCTGCTGC CCATGGTCAT CTTCAGTGAC AAGGTCAGCC CACCCAGCCT TGGCTTCCTG
    GCTGGCTACG GGATCATGGG GCTCTATGTG TCCATCGTGC TGGTTATCGG CAAGTTTGTA
    CGCGGCTTCT TCAGTGAGAT CTCCCACTCC ATCATGTTTG AGGAGCTGCC GTGTGTGGAC
    CGCATCCTCA AGCTCTGCCA GGACATCTTC CTGGTGCGGG AGACGCGGGA GCTGGAGCTG
    GAAGAGGAGC TTTATGCCAA GCTTATCTTC CTGTACCGCT CGCCTGAGAC GATGATCAAG
    TGGACCCGTG AGAAGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06942
    Clone ID Related Accession (Same CDS sequence) XM_005620574.2 , XM_005620574.1
    Accession Version XM_005620574.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product piezo-type mechanosensitive ion channel component 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620574.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAGCGGC ACGTGCTCGG CGCCGTCCTC TACTGGCTGC TGCTGCCCTT GGCACTCCTG 
    GCTGCCTGCC TGTTCCGCTA TAACGCGCTG TCTCTGGTCT ATCTGCTCTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTGTCGGCGC GGCTTCCCAG GCCACACTCG TCGCCTGCTC
    CGGGCCCTGC TGGTTTTCAG CCTCATCTTC CTGGCAGCTC ACCTGACCTT CCAGATATGC
    CTGCACACTG TGCCCCACCT GTACCAGCTT CTGGAGCCCA GCTGTGGCCC CTGGGAGACC
    CTCTCTCGGC ACATTGGGGT CACAAGGCTG GACCTGAAGG ACATCCCCAC TGCTGTCCGG
    CTGGTGGCCC CCGACCTTGG TGTCCTGGTG GTCTGCGCTG TGTGCCTCGG CCTCTGCGGG
    CGCCTCACAC AGGAAGCCCC ACGGAGCCAG CGTGCCCAGG AGCTGGACGA TGATGATGAT
    GGGGAAGTGG ACACCGGCTC CCCTGTGGGG CTTCAGGGAG CCCCCGTGCA GGCCCCCACG
    CGGAGGTCAC GGTTGGCCAC CCGGTTCCAG ATCACAGCCC ACTGGCTGTT GGTGGCCGCG
    GGAAGGACCC TGGCTATTGT GCTGCTCGCA TTGGCAGGCA TCGCCCACCC CTCTGCCTTC
    TCCAGCGTCT ACTTCCTAGT CTTCCTGGCC ACCTGCACCT GGTGGGCATG CCACTTTCCC
    ATCAGCCTCC TGGGCTTCAG CACGCTCTGC GTCACAGTGG GCTGCTTCGG TGCCGGCCAT
    CTCCTCTGCA TCTACTGCTA CCAGACGCCC TTTGCCCAGA CCGTGCTCCC TCCTGCTGGA
    ATCTGGGCCA GGGTGTTTGG TCTCAAGGCC TTGGTGACCC ACGGCAACTG CTCCAGCCCC
    AACGTGCTGG TCTTCAGTAC CAGCCACGAC TGGCCCATCT ACGTGAGCCC CGGCATCTTG
    CTGCTTCTCT ACTACACGGT GACCTCACTC CTGAAGCTCC GCACTCGCCA GCCGTTGGAC
    CAGAGGAAGG AAGTGGCCAG GAACGACGAG GAGCAGGAGG TAGAGCTGAC CCAGGTGGAC
    CAGTGGCTCC AGGACCAGGC CAGAGCTGCA GCCACCAAGG AGGAGGGGGC TGCCCAGCAC
    ATGCTGCCCA CAACCGTGGG GTCTGACTGG GAGACCGACA GCTGTACGGT GCATGACCTG
    ACTGGCCACA CCCCCGTCCG GCAGCGTCCA GTGCACCCCA GGCTGGCTGA GCCTCAAGAG
    ACATCTACCC TCCACAGTCT GGGTCATCTC ATTATGGACC AGAGCTACGT GTGTGCCCTC
    ATTGCCATGA TGGTATGGAG CATCACCTAC CATAGTTGGC TGACCTTCGT GCTGCTGCTC
    TGGGCCTGCC TCATCTGGAC GGTGCGCAGC CGCCACCAGA TGGCCATGCT CTGCTCACCC
    TTCATCCTGC TCTACGGGCT GGCTCTGTGT GGCCTGCGCT ATGTGTGGGC CATGGACCTG
    CGTCCCGAGC TGCCTACCAC CCTGGGCCCC GTCAGCCTGC GTCAGCTGGG GCTGGAGCAT
    ACCCGCTACC CTTGCCTGGA CCTTGGCGCC ATGTTGCTCT ACACCCTGAC CTTCTGGCTG
    CTGCTGCGCC GGTTTGTCAA GGAGAAGCTG CTGAAGAAGG GGAAGGTACC TGCTGCCCTG
    ACAGAGGTCA CTGTGGCTGA CACAGAGCCA ACACGGGCAC GGACGCTGCT GCAGAGCCTG
    GGGGAGCTGG TCACAGGCCT GTACGCCAAG TACTGGATCT ACGTGTGTGC CGGCATGTTC
    ATTGTGGTCA GCTTCGCCGG CCGCCTCGTC GTCTACAAGA TCGTGTACAT GCTCCTTTTC
    CTGCTCTGCC TCACCCTCTT CCAGGTCTAC TACAGCCTGT GGAGGAAGCT GCTCAAGGTG
    TTCTGGTGGC TGGTGGTGGC CTATACCATG CTGGTGCTCA TCGCCGTCTA CACTTTCCAG
    TTCCAGGACT TCCCCATGTA CTGGCGCAAC CTGACGGGCC TCACTGACGA ACAGCTGGGG
    GACCTGGGCC TGGAGCAGTT CAGCGTGTCG GAGCTTTTCT CCAGCATCCT GATCCCGGGC
    TTCTTCCTGT TGGCCTGCAT CCTGCAGCTG CACTATTTCC ACCGGCCGTT CATGCAGCTC
    ACGGACCTGG AGCATGTGCC CCCGCCTGGT GCCTGCCCCC CACGGTGGGC CCACAGGCAG
    GATGTGGTCA GTGGGACCCC GCTGCTGCAG GAGGAGGAGG ACGGGCTGAG CACCGCCTGC
    CCCCACCAGG CCATGCAGGT CACAGAAGCC AACAAGTGGG GCCTGGTGGC GGAACGGCTG
    CTGGACCTGG CTTCCGGCTT CTCGGACGTC CTCACCCGTG TGCAGGTGCT GCTGCGGCGC
    CTTCTAGAGT TGCACATCCT CAAGCTGGTG GCCCTGTACA CTGTGTGGGT GGCCCTGAAG
    GAGGTGTCCG TGATGAACCT GCTGCTGGTC GTGCTGTGGG CCTTCGCGCT GCCCTACCCC
    CGCTTCCGGC CCATGGCCTC CTGCCTGTCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTCGTAAGC CCGCACGAGT ACTCCAGCAA CTGCACCGAG
    CCCCTCCCCA ACAGCACCAA CCTGCAGAAG ATGGAGATCA AGCAGTCCTT GCTATACCGT
    GGGCCCGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCAAA CCTAGGCTAC
    ATCCAGAACC ACCTGCAGGT CCTGTTGCTG CTGGTGTTCG AGGCCATCGT CTACCGGCGT
    CAGGAGCACC ACCGTCGGCA GCACCAGCTG GCCCCACTGC CCGCCCAGGC CGTCTGTGCC
    GACGGCACCC GCCAGCGGCT GGACCAGGAC CTGCTCAGCT GCCTCAAGTA CTTTGTCAAC
    TTCTTTTTCT ACAAATTTGG GCTAGAGATA TGCTTCCTGA TGGCCGTGAA CGTGATTGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTCACACGG
    CGGCGCCGTG CAGCCATCGC CCGCCTCTGG CCCAACTACT GTCTCTTCCT GTCCCTGTTC
    TTGCTGTACC AGTACCTGCT GTGCCTGGGC ATCCCCCCGG CCCTGTGCAT CGACTACCCG
    TGGCGCTGGA GCCAGGCCAT CCCCATGAAC TCAGCACTCA TCAAGTGGCT GTACCTGCCC
    GACTTCTTCA GAACCCCCAA TTCCACCAAC CTCATCAGCG ACTTCCTGCT CCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCT GAGCGCACGG AGGAATGGCA GCACATGGCC
    GGCGTCAACA CTGACCGCCT GGAGCTGCCA TTGGGTGAGC CCAACGCCGT GCCCAACTTC
    ATCTACTGCA GGTCCTACCT CGACATGCTG AAGGTGGCTG TCTTCCGCTA CCTCTTCTGG
    CTGGTGCTGG TAGTGGTATT TGTCACGGGA GCCACCCGCG TCAGCGTCTT CGGGCTGGGC
    TACCTGTTGG CCTGTTTCTA TCTGCTGCTC TTCGGCACCA GCCTATTACA GAAGCACACA
    CGCACCCGCC TCGTGCTGTG GGACTGCCTC ATTCTCTACA ACGTCACTGT CATCATCTCT
    AAGAACATGC TCTCGCTCCT GTCCTGCGTT TTTGTGGAGC AAATGCAGAG CAACTTCTGC
    TGGGTTATCC AGCTTTTCAG CCTCGTGTGC ACCGTCAAAG GCTACTATGA TCCCAAGGAG
    ATGCTGAGCA GGGACCGGGA TTGCCTGCTG CCCGTGGAAG AGGCCGGTGT GCTCTGGGAC
    AGCATCTGCT TCCTGTTCCT TCTGCTGCAG CGGCGCGTCT TCCTCAGCCG CTACTTCCTG
    CATGTCAGCG CCGAGCTCCA GGCCACCGCC TTGCAGGCCT CCAGGGGCTT CGCGCTGTAC
    AATGCTGCCA ACTTCAAAAG CATCGATCTC CACCGTAAGG CCGAGGAGAA GTCCCTGGCC
    CAGCTGAAAA GACAGATGGA GCGCATCCGT GCCAAGCAAG AGAAGCACAG GCAGAGCCGA
    GCGGGCCGCA GCCGACCCCA GGACACCCCA GACCCCACCC AGGAGCCAGG GCCTGGCAGT
    CCAGGGGGCT CCTCCCTGCC ACGGAGACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGGGACTA CTTCCTATTT GAGTCGGATA GTGAGGAGGA GGAGGAGGCC
    CTGCCCGAGG ACCCCAGGCC GTCAGCACAG AGTGCCTTTC AGATGGCGTA CCAGGCATGG
    GTGACCAATG CGCAGACAGT GCTGAGGCAG CAGCGGGAGG AGCAGGCGAG GCAGGACCAG
    ACAGGGCAGT TGCCCACGGG AGGCGGCCCG GGACAGGAGG CAGAGCCAGC GGATGGCCCA
    GACGAGGTGG TGGCTGGCCG CAGCCACGTG ATGCAGCGAG TGCTGAGCAC TGTGCAGTTT
    CTGTGGGTGC TGGGCCAAGC GCTGGTAGAT GGGCTGACAG ACTGGCTGCA CACCTTCACA
    CGGCACCACC GCGCCATGAG TGACGTGCTG CGCGCCGAGC GCTACCTGTA CACACAGGCG
    CTGGTTGAGG GCAGAGAGGT GCACCGGAGC TTGCTGGACC AGCTCTACAC TGGTGAAGCC
    GAGGCTGCCC CCGCAGTCCC CTTGGGGGTC CGAGATGCAC CAAGTACAGC ATCAAGTGGG
    CTGGGGGCTG AGGAGCCGCT GAGCACTGCG ACAGAGGACA CTGGCAGCCC CCTGAGCACC
    GGCTACCACA CCCGCAGCAG CAGCAGCGAG GAGGTGGCCA CTGAGCCTGG GGCTTCCCTA
    CACGGCTCTC GGGAGCTTCC TGCTGGCACT CCTACCCGGA TGCGCACAGC CAGTGAGCTG
    CTCCTCAGCA GGCACCTCCG CATCCAGGAG CTGGAGGAGG CCGAGCTGTT TGAAGCAGGG
    CAGGGCCGGG CACTGCGGCT GCTGCAGGCC GCATACCAGT GTGTGGCTGC CCACTCAGAA
    CTGCTCTGCT ACTTCATCAT CATCCTCAAC CACATGGTCA CTGCCTCAGC CACATCCCTG
    GTGCTGCCTG TGCTCGTCTT CCTGTGGGCC ATGCTGTCCA TCCCCAGGCC CAGCAAGCGC
    TTCTGGATGA CGGCCATCAT CTTCACCGAG GTGTCTGTGG TCACCAAGTA CCTGTTCCAG
    TTTGGCTTCT TCCCCTGGAA CACACACACA GTGCTCCGGC GCTACGAGAA CAAGCCGTAC
    TTCCCACCGC GCATCCTGGG TGTGGAGAAG ACAGACAGCT ACATAAAGTA TGACCTGGTG
    CAGCTCATGG CCCTCTTCTT CCACCGCTCC CAGCTGCTGT GCTATGGTCT CTGGGATCAT
    GAGGAGGACC CGCTGTCCAA GGAGCATGAC CGTGGAAGTG AGAAGAAAGG GACCGAGGAG
    GAACAGGCAC CACTGGAACC CCAGACTGAG GAAGGCACAG GGCCCCAGGG CGAGCCAGTG
    GTGGCCGGGG CCCTCACCCA GGACCACATC CAGGCAGAAG CAGGGGATGG GCCCCCAGAG
    CCTCCTGTGG AGCTCAAGCC CCGAGACATA AAGCGTATCA GCCTGCGTTT CAGGAAGAGG
    CGGAGGGAGA CCACAGAGCC TGTACAACCC ACAGCCACTG AAGGTGTGGT GGTGGCCTCC
    CGCAGAGAGA AGTGGAGTCG CCCCCGGGAA AGAATGACAG CCATGGGGCT CCGACTGCAG
    ACCTTCTGCC TGGCCGTGGC CCAGAGCATG TACCGGCCCC TGCGGCGCTT CTTCGATGAC
    ATCTTGCACA CCAAGTACCG TGCAGCCACC GATGTCTACG CGCTCATGTT TCTGGCTGAT
    GTGGTCGACT TCATCATCAT CATCTTCGGC TTCTGGGCCT TTGGGAAACA CTCGGCAGCC
    ACAGACATCA CATCCTCGCT GTCGGACGAC CAGGTGCCGG AGGCCTTCCT GGTCATGCTG
    CTCATCCAGT TCAGCACCAT GGTCATTGAC CGTGCCCTCT ACCTCCGCAA GACCGTGCTG
    GGCAAGCTGG CCTTCCAGGT TGTCCTGGTG CTGGCCATCC ACCTCTGGAT GTTCTTTATT
    CTGCCCGCTG TCACCGAGAG GATGTTCAAC CAGAACGCGG TGGCTCAGCT GTGGTACTTC
    GTGAAGTGCA TCTACTTCTC CCTGTCTGCC TACCAGATCC GCTGTGGCTA TCCGACCCGT
    ATCCTTGGCA ACTTCCTCAC CAAGAAGTAC AATCACCTGA ACCTCTTCCT CTTCCAGGGG
    TTCCGGCTTG TGCCATTCCT GGTGGAGCTG CGGGCCGTGA TGGACTGGGT ATGGACAGAC
    ACCACGCTGT CCCTGTCCAG CTGGATGTGT GTGGAGGACA TCTATGCCAA CATCTTCATC
    ATCAAGTGCA GCCGTGAGAC TGAGAAGAAA TATCCCCAGC CGAAGGGGCA GAAGAAGAAG
    AAGATTGTCA AGTACGGCAT GGGCGGTCTC ATCATCCTGT TCCTCGTGGC TATCATCTGG
    TTCCCGTTGC TTTTCATGTC CCTGGTACGC TCTGTGGTTG GTGTCGTCAA CCAGCCCATC
    GATGTTACTG TCACCCTTAA GCTGGGTGGC TATGAGCCCC TCTTTACCAT GAGTGCCCAG
    CAGCCGTCCA TCGTGCCGTT CACACAGCAG GCCTATGAGG AGCTGTCCAG ACAGTTTGAC
    CCTAACCCGT TGGCCATGCA GTTCATCAGC CAGTACAGCC CCGAGGACAT CGTCACGGCA
    CAGATCGAGG GCAGTTCAGG GGCACTGTGG CGCATCAGCC CACCCAGCCG GGCCCAGATG
    AAGCGGGAGC TATACAATGG TACAGCTGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CACCGTGGAG TACACCAATG AGAAGCACAC CCTGGACTGG
    GCCCCAAATA GCACTGAGCG GCGGCAGCTG GCCAGCCTGC TTGAGGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTTCCCAAG TACATCCGTG CCCCTAATGG GCCTGAAGCC
    AACCCAGTGA AGCAGCTGCA GCCCAACGAG GAGGCCGACT ACCTAGGCGT GCGCATCCAG
    CTGCGGAGGG AGCGCGTGGG CTCAGGGGCC GCTGGCTTCC TTGAGTGGTG GGTCATCGAG
    CTGCAGGATT GCCAGGCCGA GTGCAACCTG CTGCCCATGG TCATCTTCAG TGACAAGGTC
    AGCCCACCCA GCCTTGGCTT CCTGGCTGGC TACGGGATCA TGGGGCTCTA TGTGTCCATC
    GTGCTGGTTA TCGGCAAGTT TGTACGCGGC TTCTTCAGTG AGATCTCCCA CTCCATCATG
    TTTGAGGAGC TGCCGTGTGT GGACCGCATC CTCAAGCTCT GCCAGGACAT CTTCCTGGTG
    CGGGAGACGC GGGAGCTGGA GCTGGAAGAG GAGCTTTATG CCAAGCTTAT CTTCCTGTAC
    CGCTCGCCTG AGACGATGAT CAAGTGGACC CGTGAGAAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620631.1
    CDS269..7813
    Translation

    Target ORF information:

    RefSeq Version XM_005620574.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620574.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAGCGGC ACGTGCTCGG CGCCGTCCTC TACTGGCTGC TGCTGCCCTT GGCACTCCTG 
    GCTGCCTGCC TGTTCCGCTA TAACGCGCTG TCTCTGGTCT ATCTGCTCTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTGTCGGCGC GGCTTCCCAG GCCACACTCG TCGCCTGCTC
    CGGGCCCTGC TGGTTTTCAG CCTCATCTTC CTGGCAGCTC ACCTGACCTT CCAGATATGC
    CTGCACACTG TGCCCCACCT GTACCAGCTT CTGGAGCCCA GCTGTGGCCC CTGGGAGACC
    CTCTCTCGGC ACATTGGGGT CACAAGGCTG GACCTGAAGG ACATCCCCAC TGCTGTCCGG
    CTGGTGGCCC CCGACCTTGG TGTCCTGGTG GTCTGCGCTG TGTGCCTCGG CCTCTGCGGG
    CGCCTCACAC AGGAAGCCCC ACGGAGCCAG CGTGCCCAGG AGCTGGACGA TGATGATGAT
    GGGGAAGTGG ACACCGGCTC CCCTGTGGGG CTTCAGGGAG CCCCCGTGCA GGCCCCCACG
    CGGAGGTCAC GGTTGGCCAC CCGGTTCCAG ATCACAGCCC ACTGGCTGTT GGTGGCCGCG
    GGAAGGACCC TGGCTATTGT GCTGCTCGCA TTGGCAGGCA TCGCCCACCC CTCTGCCTTC
    TCCAGCGTCT ACTTCCTAGT CTTCCTGGCC ACCTGCACCT GGTGGGCATG CCACTTTCCC
    ATCAGCCTCC TGGGCTTCAG CACGCTCTGC GTCACAGTGG GCTGCTTCGG TGCCGGCCAT
    CTCCTCTGCA TCTACTGCTA CCAGACGCCC TTTGCCCAGA CCGTGCTCCC TCCTGCTGGA
    ATCTGGGCCA GGGTGTTTGG TCTCAAGGCC TTGGTGACCC ACGGCAACTG CTCCAGCCCC
    AACGTGCTGG TCTTCAGTAC CAGCCACGAC TGGCCCATCT ACGTGAGCCC CGGCATCTTG
    CTGCTTCTCT ACTACACGGT GACCTCACTC CTGAAGCTCC GCACTCGCCA GCCGTTGGAC
    CAGAGGAAGG AAGTGGCCAG GAACGACGAG GAGCAGGAGG TAGAGCTGAC CCAGGTGGAC
    CAGTGGCTCC AGGACCAGGC CAGAGCTGCA GCCACCAAGG AGGAGGGGGC TGCCCAGCAC
    ATGCTGCCCA CAACCGTGGG GTCTGACTGG GAGACCGACA GCTGTACGGT GCATGACCTG
    ACTGGCCACA CCCCCGTCCG GCAGCGTCCA GTGCACCCCA GGCTGGCTGA GCCTCAAGAG
    ACATCTACCC TCCACAGTCT GGGTCATCTC ATTATGGACC AGAGCTACGT GTGTGCCCTC
    ATTGCCATGA TGGTATGGAG CATCACCTAC CATAGTTGGC TGACCTTCGT GCTGCTGCTC
    TGGGCCTGCC TCATCTGGAC GGTGCGCAGC CGCCACCAGA TGGCCATGCT CTGCTCACCC
    TTCATCCTGC TCTACGGGCT GGCTCTGTGT GGCCTGCGCT ATGTGTGGGC CATGGACCTG
    CGTCCCGAGC TGCCTACCAC CCTGGGCCCC GTCAGCCTGC GTCAGCTGGG GCTGGAGCAT
    ACCCGCTACC CTTGCCTGGA CCTTGGCGCC ATGTTGCTCT ACACCCTGAC CTTCTGGCTG
    CTGCTGCGCC GGTTTGTCAA GGAGAAGCTG CTGAAGAAGG GGAAGGTACC TGCTGCCCTG
    ACAGAGGTCA CTGTGGCTGA CACAGAGCCA ACACGGGCAC GGACGCTGCT GCAGAGCCTG
    GGGGAGCTGG TCACAGGCCT GTACGCCAAG TACTGGATCT ACGTGTGTGC CGGCATGTTC
    ATTGTGGTCA GCTTCGCCGG CCGCCTCGTC GTCTACAAGA TCGTGTACAT GCTCCTTTTC
    CTGCTCTGCC TCACCCTCTT CCAGGTCTAC TACAGCCTGT GGAGGAAGCT GCTCAAGGTG
    TTCTGGTGGC TGGTGGTGGC CTATACCATG CTGGTGCTCA TCGCCGTCTA CACTTTCCAG
    TTCCAGGACT TCCCCATGTA CTGGCGCAAC CTGACGGGCC TCACTGACGA ACAGCTGGGG
    GACCTGGGCC TGGAGCAGTT CAGCGTGTCG GAGCTTTTCT CCAGCATCCT GATCCCGGGC
    TTCTTCCTGT TGGCCTGCAT CCTGCAGCTG CACTATTTCC ACCGGCCGTT CATGCAGCTC
    ACGGACCTGG AGCATGTGCC CCCGCCTGGT GCCTGCCCCC CACGGTGGGC CCACAGGCAG
    GATGTGGTCA GTGGGACCCC GCTGCTGCAG GAGGAGGAGG ACGGGCTGAG CACCGCCTGC
    CCCCACCAGG CCATGCAGGT CACAGAAGCC AACAAGTGGG GCCTGGTGGC GGAACGGCTG
    CTGGACCTGG CTTCCGGCTT CTCGGACGTC CTCACCCGTG TGCAGGTGCT GCTGCGGCGC
    CTTCTAGAGT TGCACATCCT CAAGCTGGTG GCCCTGTACA CTGTGTGGGT GGCCCTGAAG
    GAGGTGTCCG TGATGAACCT GCTGCTGGTC GTGCTGTGGG CCTTCGCGCT GCCCTACCCC
    CGCTTCCGGC CCATGGCCTC CTGCCTGTCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTCGTAAGC CCGCACGAGT ACTCCAGCAA CTGCACCGAG
    CCCCTCCCCA ACAGCACCAA CCTGCAGAAG ATGGAGATCA AGCAGTCCTT GCTATACCGT
    GGGCCCGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCAAA CCTAGGCTAC
    ATCCAGAACC ACCTGCAGGT CCTGTTGCTG CTGGTGTTCG AGGCCATCGT CTACCGGCGT
    CAGGAGCACC ACCGTCGGCA GCACCAGCTG GCCCCACTGC CCGCCCAGGC CGTCTGTGCC
    GACGGCACCC GCCAGCGGCT GGACCAGGAC CTGCTCAGCT GCCTCAAGTA CTTTGTCAAC
    TTCTTTTTCT ACAAATTTGG GCTAGAGATA TGCTTCCTGA TGGCCGTGAA CGTGATTGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTCACACGG
    CGGCGCCGTG CAGCCATCGC CCGCCTCTGG CCCAACTACT GTCTCTTCCT GTCCCTGTTC
    TTGCTGTACC AGTACCTGCT GTGCCTGGGC ATCCCCCCGG CCCTGTGCAT CGACTACCCG
    TGGCGCTGGA GCCAGGCCAT CCCCATGAAC TCAGCACTCA TCAAGTGGCT GTACCTGCCC
    GACTTCTTCA GAACCCCCAA TTCCACCAAC CTCATCAGCG ACTTCCTGCT CCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCT GAGCGCACGG AGGAATGGCA GCACATGGCC
    GGCGTCAACA CTGACCGCCT GGAGCTGCCA TTGGGTGAGC CCAACGCCGT GCCCAACTTC
    ATCTACTGCA GGTCCTACCT CGACATGCTG AAGGTGGCTG TCTTCCGCTA CCTCTTCTGG
    CTGGTGCTGG TAGTGGTATT TGTCACGGGA GCCACCCGCG TCAGCGTCTT CGGGCTGGGC
    TACCTGTTGG CCTGTTTCTA TCTGCTGCTC TTCGGCACCA GCCTATTACA GAAGCACACA
    CGCACCCGCC TCGTGCTGTG GGACTGCCTC ATTCTCTACA ACGTCACTGT CATCATCTCT
    AAGAACATGC TCTCGCTCCT GTCCTGCGTT TTTGTGGAGC AAATGCAGAG CAACTTCTGC
    TGGGTTATCC AGCTTTTCAG CCTCGTGTGC ACCGTCAAAG GCTACTATGA TCCCAAGGAG
    ATGCTGAGCA GGGACCGGGA TTGCCTGCTG CCCGTGGAAG AGGCCGGTGT GCTCTGGGAC
    AGCATCTGCT TCCTGTTCCT TCTGCTGCAG CGGCGCGTCT TCCTCAGCCG CTACTTCCTG
    CATGTCAGCG CCGAGCTCCA GGCCACCGCC TTGCAGGCCT CCAGGGGCTT CGCGCTGTAC
    AATGCTGCCA ACTTCAAAAG CATCGATCTC CACCGTAAGG CCGAGGAGAA GTCCCTGGCC
    CAGCTGAAAA GACAGATGGA GCGCATCCGT GCCAAGCAAG AGAAGCACAG GCAGAGCCGA
    GCGGGCCGCA GCCGACCCCA GGACACCCCA GACCCCACCC AGGAGCCAGG GCCTGGCAGT
    CCAGGGGGCT CCTCCCTGCC ACGGAGACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGGGACTA CTTCCTATTT GAGTCGGATA GTGAGGAGGA GGAGGAGGCC
    CTGCCCGAGG ACCCCAGGCC GTCAGCACAG AGTGCCTTTC AGATGGCGTA CCAGGCATGG
    GTGACCAATG CGCAGACAGT GCTGAGGCAG CAGCGGGAGG AGCAGGCGAG GCAGGACCAG
    ACAGGGCAGT TGCCCACGGG AGGCGGCCCG GGACAGGAGG CAGAGCCAGC GGATGGCCCA
    GACGAGGTGG TGGCTGGCCG CAGCCACGTG ATGCAGCGAG TGCTGAGCAC TGTGCAGTTT
    CTGTGGGTGC TGGGCCAAGC GCTGGTAGAT GGGCTGACAG ACTGGCTGCA CACCTTCACA
    CGGCACCACC GCGCCATGAG TGACGTGCTG CGCGCCGAGC GCTACCTGTA CACACAGGCG
    CTGGTTGAGG GCAGAGAGGT GCACCGGAGC TTGCTGGACC AGCTCTACAC TGGTGAAGCC
    GAGGCTGCCC CCGCAGTCCC CTTGGGGGTC CGAGATGCAC CAAGTACAGC ATCAAGTGGG
    CTGGGGGCTG AGGAGCCGCT GAGCACTGCG ACAGAGGACA CTGGCAGCCC CCTGAGCACC
    GGCTACCACA CCCGCAGCAG CAGCAGCGAG GAGGTGGCCA CTGAGCCTGG GGCTTCCCTA
    CACGGCTCTC GGGAGCTTCC TGCTGGCACT CCTACCCGGA TGCGCACAGC CAGTGAGCTG
    CTCCTCAGCA GGCACCTCCG CATCCAGGAG CTGGAGGAGG CCGAGCTGTT TGAAGCAGGG
    CAGGGCCGGG CACTGCGGCT GCTGCAGGCC GCATACCAGT GTGTGGCTGC CCACTCAGAA
    CTGCTCTGCT ACTTCATCAT CATCCTCAAC CACATGGTCA CTGCCTCAGC CACATCCCTG
    GTGCTGCCTG TGCTCGTCTT CCTGTGGGCC ATGCTGTCCA TCCCCAGGCC CAGCAAGCGC
    TTCTGGATGA CGGCCATCAT CTTCACCGAG GTGTCTGTGG TCACCAAGTA CCTGTTCCAG
    TTTGGCTTCT TCCCCTGGAA CACACACACA GTGCTCCGGC GCTACGAGAA CAAGCCGTAC
    TTCCCACCGC GCATCCTGGG TGTGGAGAAG ACAGACAGCT ACATAAAGTA TGACCTGGTG
    CAGCTCATGG CCCTCTTCTT CCACCGCTCC CAGCTGCTGT GCTATGGTCT CTGGGATCAT
    GAGGAGGACC CGCTGTCCAA GGAGCATGAC CGTGGAAGTG AGAAGAAAGG GACCGAGGAG
    GAACAGGCAC CACTGGAACC CCAGACTGAG GAAGGCACAG GGCCCCAGGG CGAGCCAGTG
    GTGGCCGGGG CCCTCACCCA GGACCACATC CAGGCAGAAG CAGGGGATGG GCCCCCAGAG
    CCTCCTGTGG AGCTCAAGCC CCGAGACATA AAGCGTATCA GCCTGCGTTT CAGGAAGAGG
    CGGAGGGAGA CCACAGAGCC TGTACAACCC ACAGCCACTG AAGGTGTGGT GGTGGCCTCC
    CGCAGAGAGA AGTGGAGTCG CCCCCGGGAA AGAATGACAG CCATGGGGCT CCGACTGCAG
    ACCTTCTGCC TGGCCGTGGC CCAGAGCATG TACCGGCCCC TGCGGCGCTT CTTCGATGAC
    ATCTTGCACA CCAAGTACCG TGCAGCCACC GATGTCTACG CGCTCATGTT TCTGGCTGAT
    GTGGTCGACT TCATCATCAT CATCTTCGGC TTCTGGGCCT TTGGGAAACA CTCGGCAGCC
    ACAGACATCA CATCCTCGCT GTCGGACGAC CAGGTGCCGG AGGCCTTCCT GGTCATGCTG
    CTCATCCAGT TCAGCACCAT GGTCATTGAC CGTGCCCTCT ACCTCCGCAA GACCGTGCTG
    GGCAAGCTGG CCTTCCAGGT TGTCCTGGTG CTGGCCATCC ACCTCTGGAT GTTCTTTATT
    CTGCCCGCTG TCACCGAGAG GATGTTCAAC CAGAACGCGG TGGCTCAGCT GTGGTACTTC
    GTGAAGTGCA TCTACTTCTC CCTGTCTGCC TACCAGATCC GCTGTGGCTA TCCGACCCGT
    ATCCTTGGCA ACTTCCTCAC CAAGAAGTAC AATCACCTGA ACCTCTTCCT CTTCCAGGGG
    TTCCGGCTTG TGCCATTCCT GGTGGAGCTG CGGGCCGTGA TGGACTGGGT ATGGACAGAC
    ACCACGCTGT CCCTGTCCAG CTGGATGTGT GTGGAGGACA TCTATGCCAA CATCTTCATC
    ATCAAGTGCA GCCGTGAGAC TGAGAAGAAA TATCCCCAGC CGAAGGGGCA GAAGAAGAAG
    AAGATTGTCA AGTACGGCAT GGGCGGTCTC ATCATCCTGT TCCTCGTGGC TATCATCTGG
    TTCCCGTTGC TTTTCATGTC CCTGGTACGC TCTGTGGTTG GTGTCGTCAA CCAGCCCATC
    GATGTTACTG TCACCCTTAA GCTGGGTGGC TATGAGCCCC TCTTTACCAT GAGTGCCCAG
    CAGCCGTCCA TCGTGCCGTT CACACAGCAG GCCTATGAGG AGCTGTCCAG ACAGTTTGAC
    CCTAACCCGT TGGCCATGCA GTTCATCAGC CAGTACAGCC CCGAGGACAT CGTCACGGCA
    CAGATCGAGG GCAGTTCAGG GGCACTGTGG CGCATCAGCC CACCCAGCCG GGCCCAGATG
    AAGCGGGAGC TATACAATGG TACAGCTGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CACCGTGGAG TACACCAATG AGAAGCACAC CCTGGACTGG
    GCCCCAAATA GCACTGAGCG GCGGCAGCTG GCCAGCCTGC TTGAGGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTTCCCAAG TACATCCGTG CCCCTAATGG GCCTGAAGCC
    AACCCAGTGA AGCAGCTGCA GCCCAACGAG GAGGCCGACT ACCTAGGCGT GCGCATCCAG
    CTGCGGAGGG AGCGCGTGGG CTCAGGGGCC GCTGGCTTCC TTGAGTGGTG GGTCATCGAG
    CTGCAGGATT GCCAGGCCGA GTGCAACCTG CTGCCCATGG TCATCTTCAG TGACAAGGTC
    AGCCCACCCA GCCTTGGCTT CCTGGCTGGC TACGGGATCA TGGGGCTCTA TGTGTCCATC
    GTGCTGGTTA TCGGCAAGTT TGTACGCGGC TTCTTCAGTG AGATCTCCCA CTCCATCATG
    TTTGAGGAGC TGCCGTGTGT GGACCGCATC CTCAAGCTCT GCCAGGACAT CTTCCTGGTG
    CGGGAGACGC GGGAGCTGGA GCTGGAAGAG GAGCTTTATG CCAAGCTTAT CTTCCTGTAC
    CGCTCGCCTG AGACGATGAT CAAGTGGACC CGTGAGAAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06942
    Clone ID Related Accession (Same CDS sequence) XM_005620574.2 , XM_005620574.1
    Accession Version XM_005620574.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product piezo-type mechanosensitive ion channel component 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAGCGGC ACGTGCTCGG CGCCGTCCTC TACTGGCTGC TGCTGCCCTT GGCACTCCTG 
    GCTGCCTGCC TGTTCCGCTA TAACGCGCTG TCTCTGGTCT ATCTGCTCTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTGTCGGCGC GGCTTCCCAG GCCACACTCG TCGCCTGCTC
    CGGGCCCTGC TGGTTTTCAG CCTCATCTTC CTGGCAGCTC ACCTGACCTT CCAGATATGC
    CTGCACACTG TGCCCCACCT GTACCAGCTT CTGGAGCCCA GCTGTGGCCC CTGGGAGACC
    CTCTCTCGGC ACATTGGGGT CACAAGGCTG GACCTGAAGG ACATCCCCAC TGCTGTCCGG
    CTGGTGGCCC CCGACCTTGG TGTCCTGGTG GTCTGCGCTG TGTGCCTCGG CCTCTGCGGG
    CGCCTCACAC AGGAAGCCCC ACGGAGCCAG CGTGCCCAGG AGCTGGACGA TGATGATGAT
    GGGGAAGTGG ACACCGGCTC CCCTGTGGGG CTTCAGGGAG CCCCCGTGCA GGCCCCCACG
    CGGAGGTCAC GGTTGGCCAC CCGGTTCCAG ATCACAGCCC ACTGGCTGTT GGTGGCCGCG
    GGAAGGACCC TGGCTATTGT GCTGCTCGCA TTGGCAGGCA TCGCCCACCC CTCTGCCTTC
    TCCAGCGTCT ACTTCCTAGT CTTCCTGGCC ACCTGCACCT GGTGGGCATG CCACTTTCCC
    ATCAGCCTCC TGGGCTTCAG CACGCTCTGC GTCACAGTGG GCTGCTTCGG TGCCGGCCAT
    CTCCTCTGCA TCTACTGCTA CCAGACGCCC TTTGCCCAGA CCGTGCTCCC TCCTGCTGGA
    ATCTGGGCCA GGGTGTTTGG TCTCAAGGCC TTGGTGACCC ACGGCAACTG CTCCAGCCCC
    AACGTGCTGG TCTTCAGTAC CAGCCACGAC TGGCCCATCT ACGTGAGCCC CGGCATCTTG
    CTGCTTCTCT ACTACACGGT GACCTCACTC CTGAAGCTCC GCACTCGCCA GCCGTTGGAC
    CAGAGGAAGG AAGTGGCCAG GAACGACGAG GAGCAGGAGG TAGAGCTGAC CCAGGTGGAC
    CAGTGGCTCC AGGACCAGGC CAGAGCTGCA GCCACCAAGG AGGAGGGGGC TGCCCAGCAC
    ATGCTGCCCA CAACCGTGGG GTCTGACTGG GAGACCGACA GCTGTACGGT GCATGACCTG
    ACTGGCCACA CCCCCGTCCG GCAGCGTCCA GTGCACCCCA GGCTGGCTGA GCCTCAAGAG
    ACATCTACCC TCCACAGTCT GGGTCATCTC ATTATGGACC AGAGCTACGT GTGTGCCCTC
    ATTGCCATGA TGGTATGGAG CATCACCTAC CATAGTTGGC TGACCTTCGT GCTGCTGCTC
    TGGGCCTGCC TCATCTGGAC GGTGCGCAGC CGCCACCAGA TGGCCATGCT CTGCTCACCC
    TTCATCCTGC TCTACGGGCT GGCTCTGTGT GGCCTGCGCT ATGTGTGGGC CATGGACCTG
    CGTCCCGAGC TGCCTACCAC CCTGGGCCCC GTCAGCCTGC GTCAGCTGGG GCTGGAGCAT
    ACCCGCTACC CTTGCCTGGA CCTTGGCGCC ATGTTGCTCT ACACCCTGAC CTTCTGGCTG
    CTGCTGCGCC GGTTTGTCAA GGAGAAGCTG CTGAAGAAGG GGAAGGTACC TGCTGCCCTG
    ACAGAGGTCA CTGTGGCTGA CACAGAGCCA ACACGGGCAC GGACGCTGCT GCAGAGCCTG
    GGGGAGCTGG TCACAGGCCT GTACGCCAAG TACTGGATCT ACGTGTGTGC CGGCATGTTC
    ATTGTGGTCA GCTTCGCCGG CCGCCTCGTC GTCTACAAGA TCGTGTACAT GCTCCTTTTC
    CTGCTCTGCC TCACCCTCTT CCAGGTCTAC TACAGCCTGT GGAGGAAGCT GCTCAAGGTG
    TTCTGGTGGC TGGTGGTGGC CTATACCATG CTGGTGCTCA TCGCCGTCTA CACTTTCCAG
    TTCCAGGACT TCCCCATGTA CTGGCGCAAC CTGACGGGCC TCACTGACGA ACAGCTGGGG
    GACCTGGGCC TGGAGCAGTT CAGCGTGTCG GAGCTTTTCT CCAGCATCCT GATCCCGGGC
    TTCTTCCTGT TGGCCTGCAT CCTGCAGCTG CACTATTTCC ACCGGCCGTT CATGCAGCTC
    ACGGACCTGG AGCATGTGCC CCCGCCTGGT GCCTGCCCCC CACGGTGGGC CCACAGGCAG
    GATGTGGTCA GTGGGACCCC GCTGCTGCAG GAGGAGGAGG ACGGGCTGAG CACCGCCTGC
    CCCCACCAGG CCATGCAGGT CACAGAAGCC AACAAGTGGG GCCTGGTGGC GGAACGGCTG
    CTGGACCTGG CTTCCGGCTT CTCGGACGTC CTCACCCGTG TGCAGGTGCT GCTGCGGCGC
    CTTCTAGAGT TGCACATCCT CAAGCTGGTG GCCCTGTACA CTGTGTGGGT GGCCCTGAAG
    GAGGTGTCCG TGATGAACCT GCTGCTGGTC GTGCTGTGGG CCTTCGCGCT GCCCTACCCC
    CGCTTCCGGC CCATGGCCTC CTGCCTGTCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTCGTAAGC CCGCACGAGT ACTCCAGCAA CTGCACCGAG
    CCCCTCCCCA ACAGCACCAA CCTGCAGAAG ATGGAGATCA AGCAGTCCTT GCTATACCGT
    GGGCCCGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCAAA CCTAGGCTAC
    ATCCAGAACC ACCTGCAGGT CCTGTTGCTG CTGGTGTTCG AGGCCATCGT CTACCGGCGT
    CAGGAGCACC ACCGTCGGCA GCACCAGCTG GCCCCACTGC CCGCCCAGGC CGTCTGTGCC
    GACGGCACCC GCCAGCGGCT GGACCAGGAC CTGCTCAGCT GCCTCAAGTA CTTTGTCAAC
    TTCTTTTTCT ACAAATTTGG GCTAGAGATA TGCTTCCTGA TGGCCGTGAA CGTGATTGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTCACACGG
    CGGCGCCGTG CAGCCATCGC CCGCCTCTGG CCCAACTACT GTCTCTTCCT GTCCCTGTTC
    TTGCTGTACC AGTACCTGCT GTGCCTGGGC ATCCCCCCGG CCCTGTGCAT CGACTACCCG
    TGGCGCTGGA GCCAGGCCAT CCCCATGAAC TCAGCACTCA TCAAGTGGCT GTACCTGCCC
    GACTTCTTCA GAACCCCCAA TTCCACCAAC CTCATCAGCG ACTTCCTGCT CCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCT GAGCGCACGG AGGAATGGCA GCACATGGCC
    GGCGTCAACA CTGACCGCCT GGAGCTGCCA TTGGGTGAGC CCAACGCCGT GCCCAACTTC
    ATCTACTGCA GGTCCTACCT CGACATGCTG AAGGTGGCTG TCTTCCGCTA CCTCTTCTGG
    CTGGTGCTGG TAGTGGTATT TGTCACGGGA GCCACCCGCG TCAGCGTCTT CGGGCTGGGC
    TACCTGTTGG CCTGTTTCTA TCTGCTGCTC TTCGGCACCA GCCTATTACA GAAGCACACA
    CGCACCCGCC TCGTGCTGTG GGACTGCCTC ATTCTCTACA ACGTCACTGT CATCATCTCT
    AAGAACATGC TCTCGCTCCT GTCCTGCGTT TTTGTGGAGC AAATGCAGAG CAACTTCTGC
    TGGGTTATCC AGCTTTTCAG CCTCGTGTGC ACCGTCAAAG GCTACTATGA TCCCAAGGAG
    ATGCTGAGCA GGGACCGGGA TTGCCTGCTG CCCGTGGAAG AGGCCGGTGT GCTCTGGGAC
    AGCATCTGCT TCCTGTTCCT TCTGCTGCAG CGGCGCGTCT TCCTCAGCCG CTACTTCCTG
    CATGTCAGCG CCGAGCTCCA GGCCACCGCC TTGCAGGCCT CCAGGGGCTT CGCGCTGTAC
    AATGCTGCCA ACTTCAAAAG CATCGATCTC CACCGTAAGG CCGAGGAGAA GTCCCTGGCC
    CAGCTGAAAA GACAGATGGA GCGCATCCGT GCCAAGCAAG AGAAGCACAG GCAGAGCCGA
    GCGGGCCGCA GCCGACCCCA GGACACCCCA GACCCCACCC AGGAGCCAGG GCCTGGCAGT
    CCAGGGGGCT CCTCCCTGCC ACGGAGACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGGGACTA CTTCCTATTT GAGTCGGATA GTGAGGAGGA GGAGGAGGCC
    CTGCCCGAGG ACCCCAGGCC GTCAGCACAG AGTGCCTTTC AGATGGCGTA CCAGGCATGG
    GTGACCAATG CGCAGACAGT GCTGAGGCAG CAGCGGGAGG AGCAGGCGAG GCAGGACCAG
    ACAGGGCAGT TGCCCACGGG AGGCGGCCCG GGACAGGAGG CAGAGCCAGC GGATGGCCCA
    GACGAGGTGG TGGCTGGCCG CAGCCACGTG ATGCAGCGAG TGCTGAGCAC TGTGCAGTTT
    CTGTGGGTGC TGGGCCAAGC GCTGGTAGAT GGGCTGACAG ACTGGCTGCA CACCTTCACA
    CGGCACCACC GCGCCATGAG TGACGTGCTG CGCGCCGAGC GCTACCTGTA CACACAGGCG
    CTGGTTGAGG GCAGAGAGGT GCACCGGAGC TTGCTGGACC AGCTCTACAC TGGTGAAGCC
    GAGGCTGCCC CCGCAGTCCC CTTGGGGGTC CGAGATGCAC CAAGTACAGC ATCAAGTGGG
    CTGGGGGCTG AGGAGCCGCT GAGCACTGCG ACAGAGGACA CTGGCAGCCC CCTGAGCACC
    GGCTACCACA CCCGCAGCAG CAGCAGCGAG GAGGTGGCCA CTGAGCCTGG GGCTTCCCTA
    CACGGCTCTC GGGAGCTTCC TGCTGGCACT CCTACCCGGA TGCGCACAGC CAGTGAGCTG
    CTCCTCAGCA GGCACCTCCG CATCCAGGAG CTGGAGGAGG CCGAGCTGTT TGAAGCAGGG
    CAGGGCCGGG CACTGCGGCT GCTGCAGGCC GCATACCAGT GTGTGGCTGC CCACTCAGAA
    CTGCTCTGCT ACTTCATCAT CATCCTCAAC CACATGGTCA CTGCCTCAGC CACATCCCTG
    GTGCTGCCTG TGCTCGTCTT CCTGTGGGCC ATGCTGTCCA TCCCCAGGCC CAGCAAGCGC
    TTCTGGATGA CGGCCATCAT CTTCACCGAG GTGTCTGTGG TCACCAAGTA CCTGTTCCAG
    TTTGGCTTCT TCCCCTGGAA CACACACACA GTGCTCCGGC GCTACGAGAA CAAGCCGTAC
    TTCCCACCGC GCATCCTGGG TGTGGAGAAG ACAGACAGCT ACATAAAGTA TGACCTGGTG
    CAGCTCATGG CCCTCTTCTT CCACCGCTCC CAGCTGCTGT GCTATGGTCT CTGGGATCAT
    GAGGAGGACC CGCTGTCCAA GGAGCATGAC CGTGGAAGTG AGAAGAAAGG GACCGAGGAG
    GAACAGGCAC CACTGGAACC CCAGACTGAG GAAGGCACAG GGCCCCAGGG CGAGCCAGTG
    GTGGCCGGGG CCCTCACCCA GGACCACATC CAGGCAGAAG CAGGGGATGG GCCCCCAGAG
    CCTCCTGTGG AGCTCAAGCC CCGAGACATA AAGCGTATCA GCCTGCGTTT CAGGAAGAGG
    CGGAGGGAGA CCACAGAGCC TGTACAACCC ACAGCCACTG AAGGTGTGGT GGTGGCCTCC
    CGCAGAGAGA AGTGGAGTCG CCCCCGGGAA AGAATGACAG CCATGGGGCT CCGACTGCAG
    ACCTTCTGCC TGGCCGTGGC CCAGAGCATG TACCGGCCCC TGCGGCGCTT CTTCGATGAC
    ATCTTGCACA CCAAGTACCG TGCAGCCACC GATGTCTACG CGCTCATGTT TCTGGCTGAT
    GTGGTCGACT TCATCATCAT CATCTTCGGC TTCTGGGCCT TTGGGAAACA CTCGGCAGCC
    ACAGACATCA CATCCTCGCT GTCGGACGAC CAGGTGCCGG AGGCCTTCCT GGTCATGCTG
    CTCATCCAGT TCAGCACCAT GGTCATTGAC CGTGCCCTCT ACCTCCGCAA GACCGTGCTG
    GGCAAGCTGG CCTTCCAGGT TGTCCTGGTG CTGGCCATCC ACCTCTGGAT GTTCTTTATT
    CTGCCCGCTG TCACCGAGAG GATGTTCAAC CAGAACGCGG TGGCTCAGCT GTGGTACTTC
    GTGAAGTGCA TCTACTTCTC CCTGTCTGCC TACCAGATCC GCTGTGGCTA TCCGACCCGT
    ATCCTTGGCA ACTTCCTCAC CAAGAAGTAC AATCACCTGA ACCTCTTCCT CTTCCAGGGG
    TTCCGGCTTG TGCCATTCCT GGTGGAGCTG CGGGCCGTGA TGGACTGGGT ATGGACAGAC
    ACCACGCTGT CCCTGTCCAG CTGGATGTGT GTGGAGGACA TCTATGCCAA CATCTTCATC
    ATCAAGTGCA GCCGTGAGAC TGAGAAGAAA TATCCCCAGC CGAAGGGGCA GAAGAAGAAG
    AAGATTGTCA AGTACGGCAT GGGCGGTCTC ATCATCCTGT TCCTCGTGGC TATCATCTGG
    TTCCCGTTGC TTTTCATGTC CCTGGTACGC TCTGTGGTTG GTGTCGTCAA CCAGCCCATC
    GATGTTACTG TCACCCTTAA GCTGGGTGGC TATGAGCCCC TCTTTACCAT GAGTGCCCAG
    CAGCCGTCCA TCGTGCCGTT CACACAGCAG GCCTATGAGG AGCTGTCCAG ACAGTTTGAC
    CCTAACCCGT TGGCCATGCA GTTCATCAGC CAGTACAGCC CCGAGGACAT CGTCACGGCA
    CAGATCGAGG GCAGTTCAGG GGCACTGTGG CGCATCAGCC CACCCAGCCG GGCCCAGATG
    AAGCGGGAGC TATACAATGG TACAGCTGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CACCGTGGAG TACACCAATG AGAAGCACAC CCTGGACTGG
    GCCCCAAATA GCACTGAGCG GCGGCAGCTG GCCAGCCTGC TTGAGGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTTCCCAAG TACATCCGTG CCCCTAATGG GCCTGAAGCC
    AACCCAGTGA AGCAGCTGCA GCCCAACGAG GAGGCCGACT ACCTAGGCGT GCGCATCCAG
    CTGCGGAGGG AGCGCGTGGG CTCAGGGGCC GCTGGCTTCC TTGAGTGGTG GGTCATCGAG
    CTGCAGGATT GCCAGGCCGA GTGCAACCTG CTGCCCATGG TCATCTTCAG TGACAAGGTC
    AGCCCACCCA GCCTTGGCTT CCTGGCTGGC TACGGGATCA TGGGGCTCTA TGTGTCCATC
    GTGCTGGTTA TCGGCAAGTT TGTACGCGGC TTCTTCAGTG AGATCTCCCA CTCCATCATG
    TTTGAGGAGC TGCCGTGTGT GGACCGCATC CTCAAGCTCT GCCAGGACAT CTTCCTGGTG
    CGGGAGACGC GGGAGCTGGA GCTGGAAGAG GAGCTTTATG CCAAGCTTAT CTTCCTGTAC
    CGCTCGCCTG AGACGATGAT CAAGTGGACC CGTGAGAAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620631.1
    CDS269..7813
    Translation

    Target ORF information:

    RefSeq Version XM_005620574.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620574.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAGCGGC ACGTGCTCGG CGCCGTCCTC TACTGGCTGC TGCTGCCCTT GGCACTCCTG 
    GCTGCCTGCC TGTTCCGCTA TAACGCGCTG TCTCTGGTCT ATCTGCTCTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTGTCGGCGC GGCTTCCCAG GCCACACTCG TCGCCTGCTC
    CGGGCCCTGC TGGTTTTCAG CCTCATCTTC CTGGCAGCTC ACCTGACCTT CCAGATATGC
    CTGCACACTG TGCCCCACCT GTACCAGCTT CTGGAGCCCA GCTGTGGCCC CTGGGAGACC
    CTCTCTCGGC ACATTGGGGT CACAAGGCTG GACCTGAAGG ACATCCCCAC TGCTGTCCGG
    CTGGTGGCCC CCGACCTTGG TGTCCTGGTG GTCTGCGCTG TGTGCCTCGG CCTCTGCGGG
    CGCCTCACAC AGGAAGCCCC ACGGAGCCAG CGTGCCCAGG AGCTGGACGA TGATGATGAT
    GGGGAAGTGG ACACCGGCTC CCCTGTGGGG CTTCAGGGAG CCCCCGTGCA GGCCCCCACG
    CGGAGGTCAC GGTTGGCCAC CCGGTTCCAG ATCACAGCCC ACTGGCTGTT GGTGGCCGCG
    GGAAGGACCC TGGCTATTGT GCTGCTCGCA TTGGCAGGCA TCGCCCACCC CTCTGCCTTC
    TCCAGCGTCT ACTTCCTAGT CTTCCTGGCC ACCTGCACCT GGTGGGCATG CCACTTTCCC
    ATCAGCCTCC TGGGCTTCAG CACGCTCTGC GTCACAGTGG GCTGCTTCGG TGCCGGCCAT
    CTCCTCTGCA TCTACTGCTA CCAGACGCCC TTTGCCCAGA CCGTGCTCCC TCCTGCTGGA
    ATCTGGGCCA GGGTGTTTGG TCTCAAGGCC TTGGTGACCC ACGGCAACTG CTCCAGCCCC
    AACGTGCTGG TCTTCAGTAC CAGCCACGAC TGGCCCATCT ACGTGAGCCC CGGCATCTTG
    CTGCTTCTCT ACTACACGGT GACCTCACTC CTGAAGCTCC GCACTCGCCA GCCGTTGGAC
    CAGAGGAAGG AAGTGGCCAG GAACGACGAG GAGCAGGAGG TAGAGCTGAC CCAGGTGGAC
    CAGTGGCTCC AGGACCAGGC CAGAGCTGCA GCCACCAAGG AGGAGGGGGC TGCCCAGCAC
    ATGCTGCCCA CAACCGTGGG GTCTGACTGG GAGACCGACA GCTGTACGGT GCATGACCTG
    ACTGGCCACA CCCCCGTCCG GCAGCGTCCA GTGCACCCCA GGCTGGCTGA GCCTCAAGAG
    ACATCTACCC TCCACAGTCT GGGTCATCTC ATTATGGACC AGAGCTACGT GTGTGCCCTC
    ATTGCCATGA TGGTATGGAG CATCACCTAC CATAGTTGGC TGACCTTCGT GCTGCTGCTC
    TGGGCCTGCC TCATCTGGAC GGTGCGCAGC CGCCACCAGA TGGCCATGCT CTGCTCACCC
    TTCATCCTGC TCTACGGGCT GGCTCTGTGT GGCCTGCGCT ATGTGTGGGC CATGGACCTG
    CGTCCCGAGC TGCCTACCAC CCTGGGCCCC GTCAGCCTGC GTCAGCTGGG GCTGGAGCAT
    ACCCGCTACC CTTGCCTGGA CCTTGGCGCC ATGTTGCTCT ACACCCTGAC CTTCTGGCTG
    CTGCTGCGCC GGTTTGTCAA GGAGAAGCTG CTGAAGAAGG GGAAGGTACC TGCTGCCCTG
    ACAGAGGTCA CTGTGGCTGA CACAGAGCCA ACACGGGCAC GGACGCTGCT GCAGAGCCTG
    GGGGAGCTGG TCACAGGCCT GTACGCCAAG TACTGGATCT ACGTGTGTGC CGGCATGTTC
    ATTGTGGTCA GCTTCGCCGG CCGCCTCGTC GTCTACAAGA TCGTGTACAT GCTCCTTTTC
    CTGCTCTGCC TCACCCTCTT CCAGGTCTAC TACAGCCTGT GGAGGAAGCT GCTCAAGGTG
    TTCTGGTGGC TGGTGGTGGC CTATACCATG CTGGTGCTCA TCGCCGTCTA CACTTTCCAG
    TTCCAGGACT TCCCCATGTA CTGGCGCAAC CTGACGGGCC TCACTGACGA ACAGCTGGGG
    GACCTGGGCC TGGAGCAGTT CAGCGTGTCG GAGCTTTTCT CCAGCATCCT GATCCCGGGC
    TTCTTCCTGT TGGCCTGCAT CCTGCAGCTG CACTATTTCC ACCGGCCGTT CATGCAGCTC
    ACGGACCTGG AGCATGTGCC CCCGCCTGGT GCCTGCCCCC CACGGTGGGC CCACAGGCAG
    GATGTGGTCA GTGGGACCCC GCTGCTGCAG GAGGAGGAGG ACGGGCTGAG CACCGCCTGC
    CCCCACCAGG CCATGCAGGT CACAGAAGCC AACAAGTGGG GCCTGGTGGC GGAACGGCTG
    CTGGACCTGG CTTCCGGCTT CTCGGACGTC CTCACCCGTG TGCAGGTGCT GCTGCGGCGC
    CTTCTAGAGT TGCACATCCT CAAGCTGGTG GCCCTGTACA CTGTGTGGGT GGCCCTGAAG
    GAGGTGTCCG TGATGAACCT GCTGCTGGTC GTGCTGTGGG CCTTCGCGCT GCCCTACCCC
    CGCTTCCGGC CCATGGCCTC CTGCCTGTCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTCGTAAGC CCGCACGAGT ACTCCAGCAA CTGCACCGAG
    CCCCTCCCCA ACAGCACCAA CCTGCAGAAG ATGGAGATCA AGCAGTCCTT GCTATACCGT
    GGGCCCGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCAAA CCTAGGCTAC
    ATCCAGAACC ACCTGCAGGT CCTGTTGCTG CTGGTGTTCG AGGCCATCGT CTACCGGCGT
    CAGGAGCACC ACCGTCGGCA GCACCAGCTG GCCCCACTGC CCGCCCAGGC CGTCTGTGCC
    GACGGCACCC GCCAGCGGCT GGACCAGGAC CTGCTCAGCT GCCTCAAGTA CTTTGTCAAC
    TTCTTTTTCT ACAAATTTGG GCTAGAGATA TGCTTCCTGA TGGCCGTGAA CGTGATTGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTCACACGG
    CGGCGCCGTG CAGCCATCGC CCGCCTCTGG CCCAACTACT GTCTCTTCCT GTCCCTGTTC
    TTGCTGTACC AGTACCTGCT GTGCCTGGGC ATCCCCCCGG CCCTGTGCAT CGACTACCCG
    TGGCGCTGGA GCCAGGCCAT CCCCATGAAC TCAGCACTCA TCAAGTGGCT GTACCTGCCC
    GACTTCTTCA GAACCCCCAA TTCCACCAAC CTCATCAGCG ACTTCCTGCT CCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCT GAGCGCACGG AGGAATGGCA GCACATGGCC
    GGCGTCAACA CTGACCGCCT GGAGCTGCCA TTGGGTGAGC CCAACGCCGT GCCCAACTTC
    ATCTACTGCA GGTCCTACCT CGACATGCTG AAGGTGGCTG TCTTCCGCTA CCTCTTCTGG
    CTGGTGCTGG TAGTGGTATT TGTCACGGGA GCCACCCGCG TCAGCGTCTT CGGGCTGGGC
    TACCTGTTGG CCTGTTTCTA TCTGCTGCTC TTCGGCACCA GCCTATTACA GAAGCACACA
    CGCACCCGCC TCGTGCTGTG GGACTGCCTC ATTCTCTACA ACGTCACTGT CATCATCTCT
    AAGAACATGC TCTCGCTCCT GTCCTGCGTT TTTGTGGAGC AAATGCAGAG CAACTTCTGC
    TGGGTTATCC AGCTTTTCAG CCTCGTGTGC ACCGTCAAAG GCTACTATGA TCCCAAGGAG
    ATGCTGAGCA GGGACCGGGA TTGCCTGCTG CCCGTGGAAG AGGCCGGTGT GCTCTGGGAC
    AGCATCTGCT TCCTGTTCCT TCTGCTGCAG CGGCGCGTCT TCCTCAGCCG CTACTTCCTG
    CATGTCAGCG CCGAGCTCCA GGCCACCGCC TTGCAGGCCT CCAGGGGCTT CGCGCTGTAC
    AATGCTGCCA ACTTCAAAAG CATCGATCTC CACCGTAAGG CCGAGGAGAA GTCCCTGGCC
    CAGCTGAAAA GACAGATGGA GCGCATCCGT GCCAAGCAAG AGAAGCACAG GCAGAGCCGA
    GCGGGCCGCA GCCGACCCCA GGACACCCCA GACCCCACCC AGGAGCCAGG GCCTGGCAGT
    CCAGGGGGCT CCTCCCTGCC ACGGAGACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGGGACTA CTTCCTATTT GAGTCGGATA GTGAGGAGGA GGAGGAGGCC
    CTGCCCGAGG ACCCCAGGCC GTCAGCACAG AGTGCCTTTC AGATGGCGTA CCAGGCATGG
    GTGACCAATG CGCAGACAGT GCTGAGGCAG CAGCGGGAGG AGCAGGCGAG GCAGGACCAG
    ACAGGGCAGT TGCCCACGGG AGGCGGCCCG GGACAGGAGG CAGAGCCAGC GGATGGCCCA
    GACGAGGTGG TGGCTGGCCG CAGCCACGTG ATGCAGCGAG TGCTGAGCAC TGTGCAGTTT
    CTGTGGGTGC TGGGCCAAGC GCTGGTAGAT GGGCTGACAG ACTGGCTGCA CACCTTCACA
    CGGCACCACC GCGCCATGAG TGACGTGCTG CGCGCCGAGC GCTACCTGTA CACACAGGCG
    CTGGTTGAGG GCAGAGAGGT GCACCGGAGC TTGCTGGACC AGCTCTACAC TGGTGAAGCC
    GAGGCTGCCC CCGCAGTCCC CTTGGGGGTC CGAGATGCAC CAAGTACAGC ATCAAGTGGG
    CTGGGGGCTG AGGAGCCGCT GAGCACTGCG ACAGAGGACA CTGGCAGCCC CCTGAGCACC
    GGCTACCACA CCCGCAGCAG CAGCAGCGAG GAGGTGGCCA CTGAGCCTGG GGCTTCCCTA
    CACGGCTCTC GGGAGCTTCC TGCTGGCACT CCTACCCGGA TGCGCACAGC CAGTGAGCTG
    CTCCTCAGCA GGCACCTCCG CATCCAGGAG CTGGAGGAGG CCGAGCTGTT TGAAGCAGGG
    CAGGGCCGGG CACTGCGGCT GCTGCAGGCC GCATACCAGT GTGTGGCTGC CCACTCAGAA
    CTGCTCTGCT ACTTCATCAT CATCCTCAAC CACATGGTCA CTGCCTCAGC CACATCCCTG
    GTGCTGCCTG TGCTCGTCTT CCTGTGGGCC ATGCTGTCCA TCCCCAGGCC CAGCAAGCGC
    TTCTGGATGA CGGCCATCAT CTTCACCGAG GTGTCTGTGG TCACCAAGTA CCTGTTCCAG
    TTTGGCTTCT TCCCCTGGAA CACACACACA GTGCTCCGGC GCTACGAGAA CAAGCCGTAC
    TTCCCACCGC GCATCCTGGG TGTGGAGAAG ACAGACAGCT ACATAAAGTA TGACCTGGTG
    CAGCTCATGG CCCTCTTCTT CCACCGCTCC CAGCTGCTGT GCTATGGTCT CTGGGATCAT
    GAGGAGGACC CGCTGTCCAA GGAGCATGAC CGTGGAAGTG AGAAGAAAGG GACCGAGGAG
    GAACAGGCAC CACTGGAACC CCAGACTGAG GAAGGCACAG GGCCCCAGGG CGAGCCAGTG
    GTGGCCGGGG CCCTCACCCA GGACCACATC CAGGCAGAAG CAGGGGATGG GCCCCCAGAG
    CCTCCTGTGG AGCTCAAGCC CCGAGACATA AAGCGTATCA GCCTGCGTTT CAGGAAGAGG
    CGGAGGGAGA CCACAGAGCC TGTACAACCC ACAGCCACTG AAGGTGTGGT GGTGGCCTCC
    CGCAGAGAGA AGTGGAGTCG CCCCCGGGAA AGAATGACAG CCATGGGGCT CCGACTGCAG
    ACCTTCTGCC TGGCCGTGGC CCAGAGCATG TACCGGCCCC TGCGGCGCTT CTTCGATGAC
    ATCTTGCACA CCAAGTACCG TGCAGCCACC GATGTCTACG CGCTCATGTT TCTGGCTGAT
    GTGGTCGACT TCATCATCAT CATCTTCGGC TTCTGGGCCT TTGGGAAACA CTCGGCAGCC
    ACAGACATCA CATCCTCGCT GTCGGACGAC CAGGTGCCGG AGGCCTTCCT GGTCATGCTG
    CTCATCCAGT TCAGCACCAT GGTCATTGAC CGTGCCCTCT ACCTCCGCAA GACCGTGCTG
    GGCAAGCTGG CCTTCCAGGT TGTCCTGGTG CTGGCCATCC ACCTCTGGAT GTTCTTTATT
    CTGCCCGCTG TCACCGAGAG GATGTTCAAC CAGAACGCGG TGGCTCAGCT GTGGTACTTC
    GTGAAGTGCA TCTACTTCTC CCTGTCTGCC TACCAGATCC GCTGTGGCTA TCCGACCCGT
    ATCCTTGGCA ACTTCCTCAC CAAGAAGTAC AATCACCTGA ACCTCTTCCT CTTCCAGGGG
    TTCCGGCTTG TGCCATTCCT GGTGGAGCTG CGGGCCGTGA TGGACTGGGT ATGGACAGAC
    ACCACGCTGT CCCTGTCCAG CTGGATGTGT GTGGAGGACA TCTATGCCAA CATCTTCATC
    ATCAAGTGCA GCCGTGAGAC TGAGAAGAAA TATCCCCAGC CGAAGGGGCA GAAGAAGAAG
    AAGATTGTCA AGTACGGCAT GGGCGGTCTC ATCATCCTGT TCCTCGTGGC TATCATCTGG
    TTCCCGTTGC TTTTCATGTC CCTGGTACGC TCTGTGGTTG GTGTCGTCAA CCAGCCCATC
    GATGTTACTG TCACCCTTAA GCTGGGTGGC TATGAGCCCC TCTTTACCAT GAGTGCCCAG
    CAGCCGTCCA TCGTGCCGTT CACACAGCAG GCCTATGAGG AGCTGTCCAG ACAGTTTGAC
    CCTAACCCGT TGGCCATGCA GTTCATCAGC CAGTACAGCC CCGAGGACAT CGTCACGGCA
    CAGATCGAGG GCAGTTCAGG GGCACTGTGG CGCATCAGCC CACCCAGCCG GGCCCAGATG
    AAGCGGGAGC TATACAATGG TACAGCTGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CACCGTGGAG TACACCAATG AGAAGCACAC CCTGGACTGG
    GCCCCAAATA GCACTGAGCG GCGGCAGCTG GCCAGCCTGC TTGAGGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTTCCCAAG TACATCCGTG CCCCTAATGG GCCTGAAGCC
    AACCCAGTGA AGCAGCTGCA GCCCAACGAG GAGGCCGACT ACCTAGGCGT GCGCATCCAG
    CTGCGGAGGG AGCGCGTGGG CTCAGGGGCC GCTGGCTTCC TTGAGTGGTG GGTCATCGAG
    CTGCAGGATT GCCAGGCCGA GTGCAACCTG CTGCCCATGG TCATCTTCAG TGACAAGGTC
    AGCCCACCCA GCCTTGGCTT CCTGGCTGGC TACGGGATCA TGGGGCTCTA TGTGTCCATC
    GTGCTGGTTA TCGGCAAGTT TGTACGCGGC TTCTTCAGTG AGATCTCCCA CTCCATCATG
    TTTGAGGAGC TGCCGTGTGT GGACCGCATC CTCAAGCTCT GCCAGGACAT CTTCCTGGTG
    CGGGAGACGC GGGAGCTGGA GCTGGAAGAG GAGCTTTATG CCAAGCTTAT CTTCCTGTAC
    CGCTCGCCTG AGACGATGAT CAAGTGGACC CGTGAGAAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06943
    Clone ID Related Accession (Same CDS sequence) XM_005620575.1
    Accession Version XM_005620575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product piezo-type mechanosensitive ion channel component 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAGCGGC ACGTGCTCGG CGCCGTCCTC TACTGGCTGC TGCTGCCCTT GGCACTCCTG 
    GCTGCCTGCC TGTTCCGCTA TAACGCGCTG TCTCTGGTCT ATCTGCTCTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTGTCGGCGC GGCTTCCCAG GCCACACTCG TCGCCTGCTC
    CGGGCCCTGC TGGTTTTCAG CCTCATCTTC CTGGCAGCTC ACCTGACCTT CCAGATATGC
    CTGCACACTG TGCCCCACCT GTACCAGCTT CTGGAGCCCA GCTGTGGCCC CTGGGAGACC
    CTCTCTCGGC ACATTGGGGT CACAAGGCTG GACCTGAAGG ACATCCCCAC TGCTGTCCGG
    CTGGTGGCCC CCGACCTTGG TGTCCTGGTG GTCTGCGCTG TGTGCCTCGG CCTCTGCGGG
    CGCCTCACAC AGGAAGCCCC ACGGAGCCAG CGTGCCCAGG AGCTGGACGA TGATGATGAT
    GGGGAAGTGG ACACCGGCTC CCCTGTGGGG CTTCAGGGAG CCCCCGTGCA GGCCCCCACG
    CGGAGGTCAC GGTTGGCCAC CCGGTTCCAG ATCACAGCCC ACTGGCTGTT GGTGGCCGCG
    GGAAGGACCC TGGCTATTGT GCTGCTCGCA TTGGCAGGCA TCGCCCACCC CTCTGCCTTC
    TCCAGCGTCT ACTTCCTAGT CTTCCTGGCC ACCTGCACCT GGTGGGCATG CCACTTTCCC
    ATCAGCCTCC TGGGCTTCAG CACGCTCTGC GTCACAGTGG GCTGCTTCGG TGCCGGCCAT
    CTCCTCTGCA TCTACTGCTA CCAGACGCCC TTTGCCCAGA CCGTGCTCCC TCCTGCTGGA
    ATCTGGGCCA GGGTGTTTGG TCTCAAGGCC TTGGTGACCC ACGGCAACTG CTCCAGCCCC
    AACGTGCTGG TCTTCAGTAC CAGCCACGAC TGGCCCATCT ACGTGAGCCC CGGCATCTTG
    CTGCTTCTCT ACTACACGGT GACCTCACTC CTGAAGCTCC GCACTCGCCA GCCGTTGGAC
    CAGAGGAAGG AAGTGGCCAG GAACGACGAG GAGCAGGAGG TAGAGCTGAC CCAGGTGGAC
    CAGTGGCTCC AGGACCAGGC CAGAGCTGCA GCCACCAAGG AGGAGGGGGC TGCCCAGCAC
    ATGCTGCCCA CAACCGTGGG GTCTGACTGG GAGACCGACA GCTGTACGGT GCATGACCTG
    ACTGGCCACA CCCCCGTCCG GCAGCGTCCA GTGCACCCCA GGCTGGCTGA GCCTCAAGAG
    ACATCTACCC TCCACAGTCT GGGTCATCTC ATTATGGACC AGAGCTACGT GTGTGCCCTC
    ATTGCCATGA TGGTATGGAG CATCACCTAC CATAGTTGGC TGACCTTCGT GCTGCTGCTC
    TGGGCCTGCC TCATCTGGAC GGTGCGCAGC CGCCACCAGA TGGCCATGCT CTGCTCACCC
    TTCATCCTGC TCTACGGGCT GGCTCTGTGT GGCCTGCGCT ATGTGTGGGC CATGGACCTG
    CGTCCCGAGC TGCCTACCAC CCTGGGCCCC GTCAGCCTGC GTCAGCTGGG GCTGGAGCAT
    ACCCGCTACC CTTGCCTGGA CCTTGGCGCC ATGTTGCTCT ACACCCTGAC CTTCTGGCTG
    CTGCTGCGCC GGTTTGTCAA GGAGAAGCTG CTGAAGAAGG GGAAGGTACC TGCTGCCCTG
    ACAGAGGTCA CTGTGGCTGA CACAGAGCCA ACACGGGCAC GGACGCTGCT GCAGAGCCTG
    GGGGAGCTGG TCACAGGCCT GTACGCCAAG TACTGGATCT ACGTGTGTGC CGGCATGTTC
    ATTGTGGTCA GCTTCGCCGG CCGCCTCGTC GTCTACAAGA TCGTGTACAT GCTCCTTTTC
    CTGCTCTGCC TCACCCTCTT CCAGGTCTAC TACAGCCTGT GGAGGAAGCT GCTCAAGGTG
    TTCTGGTGGC TGGTGGTGGC CTATACCATG CTGGTGCTCA TCGCCGTCTA CACTTTCCAG
    TTCCAGGACT TCCCCATGTA CTGGCGCAAC CTGACGGGCC TCACTGACGA ACAGCTGGGG
    GACCTGGGCC TGGAGCAGTT CAGCGTGTCG GAGCTTTTCT CCAGCATCCT GATCCCGGGC
    TTCTTCCTGT TGGCCTGCAT CCTGCAGCTG CACTATTTCC ACCGGCCGTT CATGCAGCTC
    ACGGACCTGG AGCATGTGCC CCCGCCTGGT GCCTGCCCCC CACGGTGGGC CCACAGGCAG
    GATGTGGTCA GTGGGACCCC GCTGCTGCAG GAGGAGGAGG ACGGGCTGAG CACCGCCTGC
    CCCCACCAGG CCATGCAGGT CACAGAAGCC AACAAGTGGG GCCTGGTGGC GGAACGGCTG
    CTGGACCTGG CTTCCGGCTT CTCGGACGTC CTCACCCGTG TGCAGGTGCT GCTGCGGCGC
    CTTCTAGAGT TGCACATCCT CAAGCTGGTG GCCCTGTACA CTGTGTGGGT GGCCCTGAAG
    GAGGTGTCCG TGATGAACCT GCTGCTGGTC GTGCTGTGGG CCTTCGCGCT GCCCTACCCC
    CGCTTCCGGC CCATGGCCTC CTGCCTGTCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTCGTAAGC CCGCACGAGT ACTCCAGCAA CTGCACCGAG
    CCCCTCCCCA ACAGCACCAA CCTGCAGAAG ATGGAGATCA AGCAGTCCTT GCTATACCGT
    GGGCCCGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCAAA CCTAGGCTAC
    ATCCAGAACC ACCTGCAGGT CCTGTTGCTG CTGGTGTTCG AGGCCATCGT CTACCGGCGT
    CAGGAGCACC ACCGTCGGCA GCACCAGCTG GCCCCACTGC CCGCCCAGGC CGTCTGTGCC
    GACGGCACCC GCCAGCGGCT GGACCAGGAC CTGCTCAGCT GCCTCAAGTA CTTTGTCAAC
    TTCTTTTTCT ACAAATTTGG GCTAGAGATA TGCTTCCTGA TGGCCGTGAA CGTGATTGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTCACACGG
    CGGCGCCGTG CAGCCATCGC CCGCCTCTGG CCCAACTACT GTCTCTTCCT GTCCCTGTTC
    TTGCTGTACC AGTACCTGCT GTGCCTGGGC ATCCCCCCGG CCCTGTGCAT CGACTACCCG
    TGGCGCTGGA GCCAGGCCAT CCCCATGAAC TCAGCACTCA TCAAGTGGCT GTACCTGCCC
    GACTTCTTCA GAACCCCCAA TTCCACCAAC CTCATCAGCG ACTTCCTGCT CCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCT GAGCGCACGG AGGAATGGCA GCACATGGCC
    GGCGTCAACA CTGACCGCCT GGAGCTGCCA TTGGGTGAGC CCAACGCCGT GCCCAACTTC
    ATCTACTGCA GGTCCTACCT CGACATGCTG AAGGTGGCTG TCTTCCGCTA CCTCTTCTGG
    CTGGTGCTGG TAGTGGTATT TGTCACGGGA GCCACCCGCG TCAGCGTCTT CGGGCTGGGC
    TACCTGTTGG CCTGTTTCTA TCTGCTGCTC TTCGGCACCA GCCTATTACA GAAGCACACA
    CGCACCCGCC TCGTGCTGTG GGACTGCCTC ATTCTCTACA ACGTCACTGT CATCATCTCT
    AAGAACATGC TCTCGCTCCT GTCCTGCGTT TTTGTGGAGC AAATGCAGAG CAACTTCTGC
    TGGGTTATCC AGCTTTTCAG CCTCGTGTGC ACCGTCAAAG GCTACTATGA TCCCAAGGAG
    ATGCTGAGCA GGGACCGGGA TTGCCTGCTG CCCGTGGAAG AGGCCGGTGT GCTCTGGGAC
    AGCATCTGCT TCCTGTTCCT TCTGCTGCAG CGGCGCGTCT TCCTCAGCCG CTACTTCCTG
    CATGTCAGCG CCGAGCTCCA GGCCACCGCC TTGCAGGCCT CCAGGGGCTT CGCGCTGTAC
    AATGCTGCCA ACTTCAAAAG CATCGATCTC CACCGTAAGG CCGAGGAGAA GTCCCTGGCC
    CAGCTGAAAA GACAGATGGA GCGCATCCGT GCCAAGCAAG AGAAGCACAG GCAGAGCCGA
    GCGGGCCGCA GCCGACCCCA GGACACCCCA GACCCCACCC AGGAGCCAGG GCCTGGCAGT
    CCAGGGGGCT CCTCCCTGCC ACGGAGACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGGGACTA CTTCCTATTT GAGTCGGATA GTGAGGAGGA GGAGGAGGCC
    CTGCCCGAGG ACCCCAGGCC GTCAGCACAG AGTGCCTTTC AGATGGCGTA CCAGGCATGG
    GTGACCAATG CGCAGACAGT GCTGAGGCAG CAGCGGGAGG AGCAGGCGAG GCAGGACCAG
    ACAGGGCAGT TGCCCACGGG AGGCGGCCCG GGACAGGAGG CAGAGCCAGC GGATGGCCCA
    GACGAGGTGG TGGCTGGCCG CAGCCACGTG ATGCAGCGAG TGCTGAGCAC TGTGCAGTTT
    CTGTGGGTGC TGGGCCAAGC GCTGGTAGAT GGGCTGACAG ACTGGCTGCA CACCTTCACA
    CGGCACCACC GCGCCATGAG TGACGTGCTG CGCGCCGAGC GCTACCTGTA CACACAGGCG
    CTGGTTGAGG GCAGAGAGGT GCACCGGAGC TTGCTGGACC AGCTCTACAC TGGTGAAGCC
    GAGGCTGCCC CCGCAGTCCC CTTGGGGGTC CGAGATGCAC CAAGTACAGC ATCAAGTGGG
    CTGGGGGCTG AGGAGCCGCT GAGCACTGCG ACAGAGGACA CTGGCAGCCC CCTGAGCACC
    GGCTACCACA CCCGCAGCAG CAGCAGCGAG GAGGTGGCCA CTGAGCCTGG GGCTTCCCTA
    CACGGCTCTC GGGAGCTTCC TGCTGGCACT CCTACCCGGA TGCGCACAGC CAGTGAGCTG
    CTCCTCAGCA GGCACCTCCG CATCCAGGAG CTGGAGGAGG CCGAGCTGTT TGAAGCAGGG
    CAGGGCCGGG CACTGCGGCT GCTGCAGGCC GCATACCAGT GTGTGGCTGC CCACTCAGAA
    CTGCTCTGCT ACTTCATCAT CATCCTCAAC CACATGGTCA CTGCCTCAGC CACATCCCTG
    GTGCTGCCTG TGCTCGTCTT CCTGTGGGCC ATGCTGTCCA TCCCCAGGCC CAGCAAGCGC
    TTCTGGATGA CGGCCATCAT CTTCACCGAG GTGTCTGTGG TCACCAAGTA CCTGTTCCAG
    TTTGGCTTCT TCCCCTGGAA CACACACACA GTGCTCCGGC GCTACGAGAA CAAGCCGTAC
    TTCCCACCGC GCATCCTGGG TGTGGAGAAG ACAGACAGCT ACATAAAGTA TGACCTGGTG
    CAGCTCATGG CCCTCTTCTT CCACCGCTCC CAGCTGCTGT GCTATGGTCT CTGGGATCAT
    GAGGAGGACC CGCTGTCCAA GGAGCATGAC CGTGGAAGTG AGAAGAAAGG GACCGAGGAG
    GAACAGGCAC CACTGGAACC CCAGACTGAG GAAGGCACAG GGCCCCAGGG CGAGCCAGTG
    GTGGCCGGGG CCCTCACCCA GGACCACATC CAGGCAGAAG CAGGGGATGG GCCCCCAGAG
    CCTCCTGTGG AGCTCAAGCC CCGAGACATA AAGCGTATCA GCCTGCGTTT CAGGAAGAGG
    CGGAGGGAGA CCACAGAGCC TGTACAACCC ACAGCCACTG GTGTGGTGGT GGCCTCCCGC
    AGAGAGAAGT GGAGTCGCCC CCGGGAAAGA ATGACAGCCA TGGGGCTCCG ACTGCAGACC
    TTCTGCCTGG CCGTGGCCCA GAGCATGTAC CGGCCCCTGC GGCGCTTCTT CGATGACATC
    TTGCACACCA AGTACCGTGC AGCCACCGAT GTCTACGCGC TCATGTTTCT GGCTGATGTG
    GTCGACTTCA TCATCATCAT CTTCGGCTTC TGGGCCTTTG GGAAACACTC GGCAGCCACA
    GACATCACAT CCTCGCTGTC GGACGACCAG GTGCCGGAGG CCTTCCTGGT CATGCTGCTC
    ATCCAGTTCA GCACCATGGT CATTGACCGT GCCCTCTACC TCCGCAAGAC CGTGCTGGGC
    AAGCTGGCCT TCCAGGTTGT CCTGGTGCTG GCCATCCACC TCTGGATGTT CTTTATTCTG
    CCCGCTGTCA CCGAGAGGAT GTTCAACCAG AACGCGGTGG CTCAGCTGTG GTACTTCGTG
    AAGTGCATCT ACTTCTCCCT GTCTGCCTAC CAGATCCGCT GTGGCTATCC GACCCGTATC
    CTTGGCAACT TCCTCACCAA GAAGTACAAT CACCTGAACC TCTTCCTCTT CCAGGGGTTC
    CGGCTTGTGC CATTCCTGGT GGAGCTGCGG GCCGTGATGG ACTGGGTATG GACAGACACC
    ACGCTGTCCC TGTCCAGCTG GATGTGTGTG GAGGACATCT ATGCCAACAT CTTCATCATC
    AAGTGCAGCC GTGAGACTGA GAAGAAATAT CCCCAGCCGA AGGGGCAGAA GAAGAAGAAG
    ATTGTCAAGT ACGGCATGGG CGGTCTCATC ATCCTGTTCC TCGTGGCTAT CATCTGGTTC
    CCGTTGCTTT TCATGTCCCT GGTACGCTCT GTGGTTGGTG TCGTCAACCA GCCCATCGAT
    GTTACTGTCA CCCTTAAGCT GGGTGGCTAT GAGCCCCTCT TTACCATGAG TGCCCAGCAG
    CCGTCCATCG TGCCGTTCAC ACAGCAGGCC TATGAGGAGC TGTCCAGACA GTTTGACCCT
    AACCCGTTGG CCATGCAGTT CATCAGCCAG TACAGCCCCG AGGACATCGT CACGGCACAG
    ATCGAGGGCA GTTCAGGGGC ACTGTGGCGC ATCAGCCCAC CCAGCCGGGC CCAGATGAAG
    CGGGAGCTAT ACAATGGTAC AGCTGACATC ACCCTGCGCT TCACCTGGAA CTTCCAGAGG
    GACCTGGCCA AGGGCGGCAC CGTGGAGTAC ACCAATGAGA AGCACACCCT GGACTGGGCC
    CCAAATAGCA CTGAGCGGCG GCAGCTGGCC AGCCTGCTTG AGGGCACCTC GGACCAGTCC
    GTGGTCATCC CCCACCTCTT TCCCAAGTAC ATCCGTGCCC CTAATGGGCC TGAAGCCAAC
    CCAGTGAAGC AGCTGCAGCC CAACGAGGAG GCCGACTACC TAGGCGTGCG CATCCAGCTG
    CGGAGGGAGC GCGTGGGCTC AGGGGCCGCT GGCTTCCTTG AGTGGTGGGT CATCGAGCTG
    CAGGATTGCC AGGCCGAGTG CAACCTGCTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCACCCAGCC TTGGCTTCCT GGCTGGCTAC GGGATCATGG GGCTCTATGT GTCCATCGTG
    CTGGTTATCG GCAAGTTTGT ACGCGGCTTC TTCAGTGAGA TCTCCCACTC CATCATGTTT
    GAGGAGCTGC CGTGTGTGGA CCGCATCCTC AAGCTCTGCC AGGACATCTT CCTGGTGCGG
    GAGACGCGGG AGCTGGAGCT GGAAGAGGAG CTTTATGCCA AGCTTATCTT CCTGTACCGC
    TCGCCTGAGA CGATGATCAA GTGGACCCGT GAGAAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620632.1
    CDS269..7810
    Translation

    Target ORF information:

    RefSeq Version XM_005620575.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAGCGGC ACGTGCTCGG CGCCGTCCTC TACTGGCTGC TGCTGCCCTT GGCACTCCTG 
    GCTGCCTGCC TGTTCCGCTA TAACGCGCTG TCTCTGGTCT ATCTGCTCTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTGTCGGCGC GGCTTCCCAG GCCACACTCG TCGCCTGCTC
    CGGGCCCTGC TGGTTTTCAG CCTCATCTTC CTGGCAGCTC ACCTGACCTT CCAGATATGC
    CTGCACACTG TGCCCCACCT GTACCAGCTT CTGGAGCCCA GCTGTGGCCC CTGGGAGACC
    CTCTCTCGGC ACATTGGGGT CACAAGGCTG GACCTGAAGG ACATCCCCAC TGCTGTCCGG
    CTGGTGGCCC CCGACCTTGG TGTCCTGGTG GTCTGCGCTG TGTGCCTCGG CCTCTGCGGG
    CGCCTCACAC AGGAAGCCCC ACGGAGCCAG CGTGCCCAGG AGCTGGACGA TGATGATGAT
    GGGGAAGTGG ACACCGGCTC CCCTGTGGGG CTTCAGGGAG CCCCCGTGCA GGCCCCCACG
    CGGAGGTCAC GGTTGGCCAC CCGGTTCCAG ATCACAGCCC ACTGGCTGTT GGTGGCCGCG
    GGAAGGACCC TGGCTATTGT GCTGCTCGCA TTGGCAGGCA TCGCCCACCC CTCTGCCTTC
    TCCAGCGTCT ACTTCCTAGT CTTCCTGGCC ACCTGCACCT GGTGGGCATG CCACTTTCCC
    ATCAGCCTCC TGGGCTTCAG CACGCTCTGC GTCACAGTGG GCTGCTTCGG TGCCGGCCAT
    CTCCTCTGCA TCTACTGCTA CCAGACGCCC TTTGCCCAGA CCGTGCTCCC TCCTGCTGGA
    ATCTGGGCCA GGGTGTTTGG TCTCAAGGCC TTGGTGACCC ACGGCAACTG CTCCAGCCCC
    AACGTGCTGG TCTTCAGTAC CAGCCACGAC TGGCCCATCT ACGTGAGCCC CGGCATCTTG
    CTGCTTCTCT ACTACACGGT GACCTCACTC CTGAAGCTCC GCACTCGCCA GCCGTTGGAC
    CAGAGGAAGG AAGTGGCCAG GAACGACGAG GAGCAGGAGG TAGAGCTGAC CCAGGTGGAC
    CAGTGGCTCC AGGACCAGGC CAGAGCTGCA GCCACCAAGG AGGAGGGGGC TGCCCAGCAC
    ATGCTGCCCA CAACCGTGGG GTCTGACTGG GAGACCGACA GCTGTACGGT GCATGACCTG
    ACTGGCCACA CCCCCGTCCG GCAGCGTCCA GTGCACCCCA GGCTGGCTGA GCCTCAAGAG
    ACATCTACCC TCCACAGTCT GGGTCATCTC ATTATGGACC AGAGCTACGT GTGTGCCCTC
    ATTGCCATGA TGGTATGGAG CATCACCTAC CATAGTTGGC TGACCTTCGT GCTGCTGCTC
    TGGGCCTGCC TCATCTGGAC GGTGCGCAGC CGCCACCAGA TGGCCATGCT CTGCTCACCC
    TTCATCCTGC TCTACGGGCT GGCTCTGTGT GGCCTGCGCT ATGTGTGGGC CATGGACCTG
    CGTCCCGAGC TGCCTACCAC CCTGGGCCCC GTCAGCCTGC GTCAGCTGGG GCTGGAGCAT
    ACCCGCTACC CTTGCCTGGA CCTTGGCGCC ATGTTGCTCT ACACCCTGAC CTTCTGGCTG
    CTGCTGCGCC GGTTTGTCAA GGAGAAGCTG CTGAAGAAGG GGAAGGTACC TGCTGCCCTG
    ACAGAGGTCA CTGTGGCTGA CACAGAGCCA ACACGGGCAC GGACGCTGCT GCAGAGCCTG
    GGGGAGCTGG TCACAGGCCT GTACGCCAAG TACTGGATCT ACGTGTGTGC CGGCATGTTC
    ATTGTGGTCA GCTTCGCCGG CCGCCTCGTC GTCTACAAGA TCGTGTACAT GCTCCTTTTC
    CTGCTCTGCC TCACCCTCTT CCAGGTCTAC TACAGCCTGT GGAGGAAGCT GCTCAAGGTG
    TTCTGGTGGC TGGTGGTGGC CTATACCATG CTGGTGCTCA TCGCCGTCTA CACTTTCCAG
    TTCCAGGACT TCCCCATGTA CTGGCGCAAC CTGACGGGCC TCACTGACGA ACAGCTGGGG
    GACCTGGGCC TGGAGCAGTT CAGCGTGTCG GAGCTTTTCT CCAGCATCCT GATCCCGGGC
    TTCTTCCTGT TGGCCTGCAT CCTGCAGCTG CACTATTTCC ACCGGCCGTT CATGCAGCTC
    ACGGACCTGG AGCATGTGCC CCCGCCTGGT GCCTGCCCCC CACGGTGGGC CCACAGGCAG
    GATGTGGTCA GTGGGACCCC GCTGCTGCAG GAGGAGGAGG ACGGGCTGAG CACCGCCTGC
    CCCCACCAGG CCATGCAGGT CACAGAAGCC AACAAGTGGG GCCTGGTGGC GGAACGGCTG
    CTGGACCTGG CTTCCGGCTT CTCGGACGTC CTCACCCGTG TGCAGGTGCT GCTGCGGCGC
    CTTCTAGAGT TGCACATCCT CAAGCTGGTG GCCCTGTACA CTGTGTGGGT GGCCCTGAAG
    GAGGTGTCCG TGATGAACCT GCTGCTGGTC GTGCTGTGGG CCTTCGCGCT GCCCTACCCC
    CGCTTCCGGC CCATGGCCTC CTGCCTGTCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTCGTAAGC CCGCACGAGT ACTCCAGCAA CTGCACCGAG
    CCCCTCCCCA ACAGCACCAA CCTGCAGAAG ATGGAGATCA AGCAGTCCTT GCTATACCGT
    GGGCCCGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCAAA CCTAGGCTAC
    ATCCAGAACC ACCTGCAGGT CCTGTTGCTG CTGGTGTTCG AGGCCATCGT CTACCGGCGT
    CAGGAGCACC ACCGTCGGCA GCACCAGCTG GCCCCACTGC CCGCCCAGGC CGTCTGTGCC
    GACGGCACCC GCCAGCGGCT GGACCAGGAC CTGCTCAGCT GCCTCAAGTA CTTTGTCAAC
    TTCTTTTTCT ACAAATTTGG GCTAGAGATA TGCTTCCTGA TGGCCGTGAA CGTGATTGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTCACACGG
    CGGCGCCGTG CAGCCATCGC CCGCCTCTGG CCCAACTACT GTCTCTTCCT GTCCCTGTTC
    TTGCTGTACC AGTACCTGCT GTGCCTGGGC ATCCCCCCGG CCCTGTGCAT CGACTACCCG
    TGGCGCTGGA GCCAGGCCAT CCCCATGAAC TCAGCACTCA TCAAGTGGCT GTACCTGCCC
    GACTTCTTCA GAACCCCCAA TTCCACCAAC CTCATCAGCG ACTTCCTGCT CCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCT GAGCGCACGG AGGAATGGCA GCACATGGCC
    GGCGTCAACA CTGACCGCCT GGAGCTGCCA TTGGGTGAGC CCAACGCCGT GCCCAACTTC
    ATCTACTGCA GGTCCTACCT CGACATGCTG AAGGTGGCTG TCTTCCGCTA CCTCTTCTGG
    CTGGTGCTGG TAGTGGTATT TGTCACGGGA GCCACCCGCG TCAGCGTCTT CGGGCTGGGC
    TACCTGTTGG CCTGTTTCTA TCTGCTGCTC TTCGGCACCA GCCTATTACA GAAGCACACA
    CGCACCCGCC TCGTGCTGTG GGACTGCCTC ATTCTCTACA ACGTCACTGT CATCATCTCT
    AAGAACATGC TCTCGCTCCT GTCCTGCGTT TTTGTGGAGC AAATGCAGAG CAACTTCTGC
    TGGGTTATCC AGCTTTTCAG CCTCGTGTGC ACCGTCAAAG GCTACTATGA TCCCAAGGAG
    ATGCTGAGCA GGGACCGGGA TTGCCTGCTG CCCGTGGAAG AGGCCGGTGT GCTCTGGGAC
    AGCATCTGCT TCCTGTTCCT TCTGCTGCAG CGGCGCGTCT TCCTCAGCCG CTACTTCCTG
    CATGTCAGCG CCGAGCTCCA GGCCACCGCC TTGCAGGCCT CCAGGGGCTT CGCGCTGTAC
    AATGCTGCCA ACTTCAAAAG CATCGATCTC CACCGTAAGG CCGAGGAGAA GTCCCTGGCC
    CAGCTGAAAA GACAGATGGA GCGCATCCGT GCCAAGCAAG AGAAGCACAG GCAGAGCCGA
    GCGGGCCGCA GCCGACCCCA GGACACCCCA GACCCCACCC AGGAGCCAGG GCCTGGCAGT
    CCAGGGGGCT CCTCCCTGCC ACGGAGACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGGGACTA CTTCCTATTT GAGTCGGATA GTGAGGAGGA GGAGGAGGCC
    CTGCCCGAGG ACCCCAGGCC GTCAGCACAG AGTGCCTTTC AGATGGCGTA CCAGGCATGG
    GTGACCAATG CGCAGACAGT GCTGAGGCAG CAGCGGGAGG AGCAGGCGAG GCAGGACCAG
    ACAGGGCAGT TGCCCACGGG AGGCGGCCCG GGACAGGAGG CAGAGCCAGC GGATGGCCCA
    GACGAGGTGG TGGCTGGCCG CAGCCACGTG ATGCAGCGAG TGCTGAGCAC TGTGCAGTTT
    CTGTGGGTGC TGGGCCAAGC GCTGGTAGAT GGGCTGACAG ACTGGCTGCA CACCTTCACA
    CGGCACCACC GCGCCATGAG TGACGTGCTG CGCGCCGAGC GCTACCTGTA CACACAGGCG
    CTGGTTGAGG GCAGAGAGGT GCACCGGAGC TTGCTGGACC AGCTCTACAC TGGTGAAGCC
    GAGGCTGCCC CCGCAGTCCC CTTGGGGGTC CGAGATGCAC CAAGTACAGC ATCAAGTGGG
    CTGGGGGCTG AGGAGCCGCT GAGCACTGCG ACAGAGGACA CTGGCAGCCC CCTGAGCACC
    GGCTACCACA CCCGCAGCAG CAGCAGCGAG GAGGTGGCCA CTGAGCCTGG GGCTTCCCTA
    CACGGCTCTC GGGAGCTTCC TGCTGGCACT CCTACCCGGA TGCGCACAGC CAGTGAGCTG
    CTCCTCAGCA GGCACCTCCG CATCCAGGAG CTGGAGGAGG CCGAGCTGTT TGAAGCAGGG
    CAGGGCCGGG CACTGCGGCT GCTGCAGGCC GCATACCAGT GTGTGGCTGC CCACTCAGAA
    CTGCTCTGCT ACTTCATCAT CATCCTCAAC CACATGGTCA CTGCCTCAGC CACATCCCTG
    GTGCTGCCTG TGCTCGTCTT CCTGTGGGCC ATGCTGTCCA TCCCCAGGCC CAGCAAGCGC
    TTCTGGATGA CGGCCATCAT CTTCACCGAG GTGTCTGTGG TCACCAAGTA CCTGTTCCAG
    TTTGGCTTCT TCCCCTGGAA CACACACACA GTGCTCCGGC GCTACGAGAA CAAGCCGTAC
    TTCCCACCGC GCATCCTGGG TGTGGAGAAG ACAGACAGCT ACATAAAGTA TGACCTGGTG
    CAGCTCATGG CCCTCTTCTT CCACCGCTCC CAGCTGCTGT GCTATGGTCT CTGGGATCAT
    GAGGAGGACC CGCTGTCCAA GGAGCATGAC CGTGGAAGTG AGAAGAAAGG GACCGAGGAG
    GAACAGGCAC CACTGGAACC CCAGACTGAG GAAGGCACAG GGCCCCAGGG CGAGCCAGTG
    GTGGCCGGGG CCCTCACCCA GGACCACATC CAGGCAGAAG CAGGGGATGG GCCCCCAGAG
    CCTCCTGTGG AGCTCAAGCC CCGAGACATA AAGCGTATCA GCCTGCGTTT CAGGAAGAGG
    CGGAGGGAGA CCACAGAGCC TGTACAACCC ACAGCCACTG GTGTGGTGGT GGCCTCCCGC
    AGAGAGAAGT GGAGTCGCCC CCGGGAAAGA ATGACAGCCA TGGGGCTCCG ACTGCAGACC
    TTCTGCCTGG CCGTGGCCCA GAGCATGTAC CGGCCCCTGC GGCGCTTCTT CGATGACATC
    TTGCACACCA AGTACCGTGC AGCCACCGAT GTCTACGCGC TCATGTTTCT GGCTGATGTG
    GTCGACTTCA TCATCATCAT CTTCGGCTTC TGGGCCTTTG GGAAACACTC GGCAGCCACA
    GACATCACAT CCTCGCTGTC GGACGACCAG GTGCCGGAGG CCTTCCTGGT CATGCTGCTC
    ATCCAGTTCA GCACCATGGT CATTGACCGT GCCCTCTACC TCCGCAAGAC CGTGCTGGGC
    AAGCTGGCCT TCCAGGTTGT CCTGGTGCTG GCCATCCACC TCTGGATGTT CTTTATTCTG
    CCCGCTGTCA CCGAGAGGAT GTTCAACCAG AACGCGGTGG CTCAGCTGTG GTACTTCGTG
    AAGTGCATCT ACTTCTCCCT GTCTGCCTAC CAGATCCGCT GTGGCTATCC GACCCGTATC
    CTTGGCAACT TCCTCACCAA GAAGTACAAT CACCTGAACC TCTTCCTCTT CCAGGGGTTC
    CGGCTTGTGC CATTCCTGGT GGAGCTGCGG GCCGTGATGG ACTGGGTATG GACAGACACC
    ACGCTGTCCC TGTCCAGCTG GATGTGTGTG GAGGACATCT ATGCCAACAT CTTCATCATC
    AAGTGCAGCC GTGAGACTGA GAAGAAATAT CCCCAGCCGA AGGGGCAGAA GAAGAAGAAG
    ATTGTCAAGT ACGGCATGGG CGGTCTCATC ATCCTGTTCC TCGTGGCTAT CATCTGGTTC
    CCGTTGCTTT TCATGTCCCT GGTACGCTCT GTGGTTGGTG TCGTCAACCA GCCCATCGAT
    GTTACTGTCA CCCTTAAGCT GGGTGGCTAT GAGCCCCTCT TTACCATGAG TGCCCAGCAG
    CCGTCCATCG TGCCGTTCAC ACAGCAGGCC TATGAGGAGC TGTCCAGACA GTTTGACCCT
    AACCCGTTGG CCATGCAGTT CATCAGCCAG TACAGCCCCG AGGACATCGT CACGGCACAG
    ATCGAGGGCA GTTCAGGGGC ACTGTGGCGC ATCAGCCCAC CCAGCCGGGC CCAGATGAAG
    CGGGAGCTAT ACAATGGTAC AGCTGACATC ACCCTGCGCT TCACCTGGAA CTTCCAGAGG
    GACCTGGCCA AGGGCGGCAC CGTGGAGTAC ACCAATGAGA AGCACACCCT GGACTGGGCC
    CCAAATAGCA CTGAGCGGCG GCAGCTGGCC AGCCTGCTTG AGGGCACCTC GGACCAGTCC
    GTGGTCATCC CCCACCTCTT TCCCAAGTAC ATCCGTGCCC CTAATGGGCC TGAAGCCAAC
    CCAGTGAAGC AGCTGCAGCC CAACGAGGAG GCCGACTACC TAGGCGTGCG CATCCAGCTG
    CGGAGGGAGC GCGTGGGCTC AGGGGCCGCT GGCTTCCTTG AGTGGTGGGT CATCGAGCTG
    CAGGATTGCC AGGCCGAGTG CAACCTGCTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCACCCAGCC TTGGCTTCCT GGCTGGCTAC GGGATCATGG GGCTCTATGT GTCCATCGTG
    CTGGTTATCG GCAAGTTTGT ACGCGGCTTC TTCAGTGAGA TCTCCCACTC CATCATGTTT
    GAGGAGCTGC CGTGTGTGGA CCGCATCCTC AAGCTCTGCC AGGACATCTT CCTGGTGCGG
    GAGACGCGGG AGCTGGAGCT GGAAGAGGAG CTTTATGCCA AGCTTATCTT CCTGTACCGC
    TCGCCTGAGA CGATGATCAA GTGGACCCGT GAGAAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06944
    Clone ID Related Accession (Same CDS sequence) XM_005620576.1
    Accession Version XM_005620576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product piezo-type mechanosensitive ion channel component 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGAGCGGC ACGTGCTCGG CGCCGTCCTC TACTGGCTGC TGCTGCCCTT GGCACTCCTG 
    GCTGCCTGCC TGTTCCGCTA TAACGCGCTG TCTCTGGTCT ATCTGCTCTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTGTCGGCGC GGCTTCCCAG GCCACACTCG TCGCCTGCTC
    CGGGCCCTGC TGGTTTTCAG CCTCATCTTC CTGGCAGCTC ACCTGACCTT CCAGATATGC
    CTGCACACTG TGCCCCACCT GTACCAGCTT CTGGAGCCCA GCTGTGGCCC CTGGGAGACC
    CTCTCTCGGC ACATTGGGGT CACAAGGCTG GACCTGAAGG ACATCCCCAC TGCTGTCCGG
    CTGGTGGCCC CCGACCTTGG TGTCCTGGTG GTCTGCGCTG TGTGCCTCGG CCTCTGCGGG
    CGCCTCACAC AGGAAGCCCC ACGGAGCCAG CGTGCCCAGG AGCTGGACGA TGATGATGAT
    GGGGAAGTGG ACACCGGCTC CCCTGTGGGG CTTCAGGGAG CCCCCGTGCA GGCCCCCACG
    CGGAGGTCAC GGTTGGCCAC CCGGTTCCAG ATCACAGCCC ACTGGCTGTT GGTGGCCGCG
    GGAAGGACCC TGGCTATTGT GCTGCTCGCA TTGGCAGGCA TCGCCCACCC CTCTGCCTTC
    TCCAGCGTCT ACTTCCTAGT CTTCCTGGCC ACCTGCACCT GGTGGGCATG CCACTTTCCC
    ATCAGCCTCC TGGGCTTCAG CACGCTCTGC GTCACAGTGG GCTGCTTCGG TGCCGGCCAT
    CTCCTCTGCA TCTACTGCTA CCAGACGCCC TTTGCCCAGA CCGTGCTCCC TCCTGCTGGA
    ATCTGGGCCA GGGTGTTTGG TCTCAAGGCC TTGGTGACCC ACGGCAACTG CTCCAGCCCC
    AACGTGCTGG TCTTCAGTAC CAGCCACGAC TGGCCCATCT ACGTGAGCCC CGGCATCTTG
    CTGCTTCTCT ACTACACGGT GACCTCACTC CTGAAGCTCC GCACTCGCCA GCCGTTGGAC
    CAGAGGAAGG AAGTGGCCAG GAACGACGAG GAGCAGGAGG TAGAGCTGAC CCAGGTGGAC
    CAGTGGCTCC AGGACCAGGC CAGAGCTGCA GCCACCAAGG AGGAGGGGGC TGCCCAGCAC
    ATGCTGCCCA CAACCGTGGG GTCTGACTGG GAGACCGACA GCTGTACGGT GCATGACCTG
    ACTGGCCACA CCCCCGTCCG GCAGCGTCCA GTGCACCCCA GGCTGGCTGA GCCTCAAGAG
    ACATCTACCC TCCACAGTCT GGGTCATCTC ATTATGGACC AGAGCTACGT GTGTGCCCTC
    ATTGCCATGA TGGTATGGAG CATCACCTAC CATAGTTGGC TGACCTTCGT GCTGCTGCTC
    TGGGCCTGCC TCATCTGGAC GGTGCGCAGC CGCCACCAGA TGGCCATGCT CTGCTCACCC
    TTCATCCTGC TCTACGGGCT GGCTCTGTGT GGCCTGCGCT ATGTGTGGGC CATGGACCTG
    CGTCCCGAGC TGCCTACCAC CCTGGGCCCC GTCAGCCTGC GTCAGCTGGG GCTGGAGCAT
    ACCCGCTACC CTTGCCTGGA CCTTGGCGCC ATGTTGCTCT ACACCCTGAC CTTCTGGCTG
    CTGCTGCGCC GGTTTGTCAA GGAGAAGCTG CTGAAGAAGG GGAAGGTACC TGCTGCCCTG
    ACAGAGGTCA CTGTGGCTGA CACAGAGCCA ACACGGGCAC GGACGCTGCT GCAGAGCCTG
    GGGGAGCTGG TCACAGGCCT GTACGCCAAG TACTGGATCT ACGTGTGTGC CGGCATGTTC
    ATTGTGGTCA GCTTCGCCGG CCGCCTCGTC GTCTACAAGA TCGTGTACAT GCTCCTTTTC
    CTGCTCTGCC TCACCCTCTT CCAGGTCTAC TACAGCCTGT GGAGGAAGCT GCTCAAGGTG
    TTCTGGTGGC TGGTGGTGGC CTATACCATG CTGGTGCTCA TCGCCGTCTA CACTTTCCAG
    TTCCAGGACT TCCCCATGTA CTGGCGCAAC CTGACGGGCC TCACTGACGA ACAGCTGGGG
    GACCTGGGCC TGGAGCAGTT CAGCGTGTCG GAGCTTTTCT CCAGCATCCT GATCCCGGGC
    TTCTTCCTGT TGGCCTGCAT CCTGCAGCTG CACTATTTCC ACCGGCCGTT CATGCAGCTC
    ACGGACCTGG AGCATGTGCC CCCGCCTGGT GCCTGCCCCC CACGGTGGGC CCACAGGCAG
    GATGTGGTCA GTGGGACCCC GCTGCTGCAG GAGGAGGAGG ACGGGCTGAG CACCGCCTGC
    CCCCACCAGG CCATGCAGGT CACAGAAGCC AACAAGTGGG GCCTGGTGGC GGAACGGCTG
    CTGGACCTGG CTTCCGGCTT CTCGGACGTC CTCACCCGTG TGCAGGTGCT GCTGCGGCGC
    CTTCTAGAGT TGCACATCCT CAAGCTGGTG GCCCTGTACA CTGTGTGGGT GGCCCTGAAG
    GAGGTGTCCG TGATGAACCT GCTGCTGGTC GTGCTGTGGG CCTTCGCGCT GCCCTACCCC
    CGCTTCCGGC CCATGGCCTC CTGCCTGTCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTCGTAAGC CCGCACGAGT ACTCCAGCAA CTGCACCGAG
    CCCCTCCCCA ACAGCACCAA CCTGCAGAAG ATGGAGATCA AGCAGTCCTT GCTATACCGT
    GGGCCCGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCAAA CCTAGGCTAC
    ATCCAGAACC ACCTGCAGGT CCTGTTGCTG CTGGTGTTCG AGGCCATCGT CTACCGGCGT
    CAGGAGCACC ACCGTCGGCA GCACCAGCTG GCCCCACTGC CCGCCCAGGC CGTCTGTGCC
    GACGGCACCC GCCAGCGGCT GGACCAGGAC CTGCTCAGCT GCCTCAAGTA CTTTGTCAAC
    TTCTTTTTCT ACAAATTTGG GCTAGAGATA TGCTTCCTGA TGGCCGTGAA CGTGATTGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTCACACGG
    CGGCGCCGTG CAGCCATCGC CCGCCTCTGG CCCAACTACT GTCTCTTCCT GTCCCTGTTC
    TTGCTGTACC AGTACCTGCT GTGCCTGGGC ATCCCCCCGG CCCTGTGCAT CGACTACCCG
    TGGCGCTGGA GCCAGGCCAT CCCCATGAAC TCAGCACTCA TCAAGTGGCT GTACCTGCCC
    GACTTCTTCA GAACCCCCAA TTCCACCAAC CTCATCAGCG ACTTCCTGCT CCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCT GAGCGCACGG AGGAATGGCA GCACATGGCC
    GGCGTCAACA CTGACCGCCT GGAGCTGCCA TTGGGTGAGC CCAACGCCGT GCCCAACTTC
    ATCTACTGCA GGTCCTACCT CGACATGCTG AAGGTGGCTG TCTTCCGCTA CCTCTTCTGG
    CTGGTGCTGG TAGTGGTATT TGTCACGGGA GCCACCCGCG TCAGCGTCTT CGGGCTGGGC
    TACCTGTTGG CCTGTTTCTA TCTGCTGCTC TTCGGCACCA GCCTATTACA GAAGCACACA
    CGCACCCGCC TCGTGCTGTG GGACTGCCTC ATTCTCTACA ACGTCACTGT CATCATCTCT
    AAGAACATGC TCTCGCTCCT GTCCTGCGTT TTTGTGGAGC AAATGCAGAG CAACTTCTGC
    TGGGTTATCC AGCTTTTCAG CCTCGTGTGC ACCGTCAAAG GCTACTATGA TCCCAAGGAG
    ATGCTGAGCA GGGACCGGGA TTGCCTGCTG CCCGTGGAAG AGGCCGGTGT GCTCTGGGAC
    AGCATCTGCT TCCTGTTCCT TCTGCTGCAG CGGCGCGTCT TCCTCAGCCG CTACTTCCTG
    CATGTCAGCG CCGAGCTCCA GGCCACCGCC TTGCAGGCCT CCAGGGGCTT CGCGCTGTAC
    AATGCTGCCA ACTTCAAAAG CATCGATCTC CACCGTAAGG CCGAGGAGAA GTCCCTGGCC
    CAGCTGAAAA GACAGATGGA GCGCATCCGT GCCAAGCAAG AGAAGCACAG GCAGAGCCGA
    GCGGGCCGCA GCCGACCCCA GGACACCCCA GACCCCACCC AGGAGCCAGG GCCTGGCAGT
    CCAGGGGGCT CCTCCCTGCC ACGGAGACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGGGACTA CTTCCTATTT GAGTCGGATA GTGAGGAGGA GGAGGAGGCC
    CTGCCCGAGG ACCCCAGGCC GTCAGCACAG AGTGCCTTTC AGATGGCGTA CCAGGCATGG
    GTGACCAATG CGCAGACAGT GCTGAGGCAG CAGCGGGAGG AGCAGGCGAG GCAGGACCAG
    ACAGGGCAGT TGCCCACGGG AGGCGGCCCG GGACAGGAGG CAGAGCCAGC GGATGGCCCA
    GACGAGGTGG TGGCTGGCCG CAGCCACGTG ATGCAGCGAG TGCTGAGCAC TGTGCAGTTT
    CTGTGGGTGC TGGGCCAAGC GCTGGTAGAT GGGCTGACAG ACTGGCTGCA CACCTTCACA
    CGGCACCACC GCGCCATGAG TGACGTGCTG CGCGCCGAGC GCTACCTGTA CACACAGGCG
    CTGGTTGAGG GCAGAGAGGT GCACCGGAGC TTGCTGGACC AGCTCTACAC TGGTGAAGCC
    GAGGCTGCCC CCGCAGTCCC CTTGGGGGTC CGAGATGCAC CAAGTACAGC ATCAAGCAGC
    AGCAGCGAGG AGGTGGCCAC TGAGCCTGGG GCTTCCCTAC ACGGCTCTCG GGAGCTTCCT
    GCTGGCACTC CTACCCGGAT GCGCACAGCC AGTGAGCTGC TCCTCAGCAG GCACCTCCGC
    ATCCAGGAGC TGGAGGAGGC CGAGCTGTTT GAAGCAGGGC AGGGCCGGGC ACTGCGGCTG
    CTGCAGGCCG CATACCAGTG TGTGGCTGCC CACTCAGAAC TGCTCTGCTA CTTCATCATC
    ATCCTCAACC ACATGGTCAC TGCCTCAGCC ACATCCCTGG TGCTGCCTGT GCTCGTCTTC
    CTGTGGGCCA TGCTGTCCAT CCCCAGGCCC AGCAAGCGCT TCTGGATGAC GGCCATCATC
    TTCACCGAGG TGTCTGTGGT CACCAAGTAC CTGTTCCAGT TTGGCTTCTT CCCCTGGAAC
    ACACACACAG TGCTCCGGCG CTACGAGAAC AAGCCGTACT TCCCACCGCG CATCCTGGGT
    GTGGAGAAGA CAGACAGCTA CATAAAGTAT GACCTGGTGC AGCTCATGGC CCTCTTCTTC
    CACCGCTCCC AGCTGCTGTG CTATGGTCTC TGGGATCATG AGGAGGACCC GCTGTCCAAG
    GAGCATGACC GTGGAAGTGA GAAGAAAGGG ACCGAGGAGG AACAGGCACC ACTGGAACCC
    CAGACTGAGG AAGGCACAGG GCCCCAGGGC GAGCCAGTGG TGGCCGGGGC CCTCACCCAG
    GACCACATCC AGGCAGAAGC AGGGGATGGG CCCCCAGAGC CTCCTGTGGA GCTCAAGCCC
    CGAGACATAA AGCGTATCAG CCTGCGTTTC AGGAAGAGGC GGAGGGAGAC CACAGAGCCT
    GTACAACCCA CAGCCACTGA AGGTGTGGTG GTGGCCTCCC GCAGAGAGAA GTGGAGTCGC
    CCCCGGGAAA GAATGACAGC CATGGGGCTC CGACTGCAGA CCTTCTGCCT GGCCGTGGCC
    CAGAGCATGT ACCGGCCCCT GCGGCGCTTC TTCGATGACA TCTTGCACAC CAAGTACCGT
    GCAGCCACCG ATGTCTACGC GCTCATGTTT CTGGCTGATG TGGTCGACTT CATCATCATC
    ATCTTCGGCT TCTGGGCCTT TGGGAAACAC TCGGCAGCCA CAGACATCAC ATCCTCGCTG
    TCGGACGACC AGGTGCCGGA GGCCTTCCTG GTCATGCTGC TCATCCAGTT CAGCACCATG
    GTCATTGACC GTGCCCTCTA CCTCCGCAAG ACCGTGCTGG GCAAGCTGGC CTTCCAGGTT
    GTCCTGGTGC TGGCCATCCA CCTCTGGATG TTCTTTATTC TGCCCGCTGT CACCGAGAGG
    ATGTTCAACC AGAACGCGGT GGCTCAGCTG TGGTACTTCG TGAAGTGCAT CTACTTCTCC
    CTGTCTGCCT ACCAGATCCG CTGTGGCTAT CCGACCCGTA TCCTTGGCAA CTTCCTCACC
    AAGAAGTACA ATCACCTGAA CCTCTTCCTC TTCCAGGGGT TCCGGCTTGT GCCATTCCTG
    GTGGAGCTGC GGGCCGTGAT GGACTGGGTA TGGACAGACA CCACGCTGTC CCTGTCCAGC
    TGGATGTGTG TGGAGGACAT CTATGCCAAC ATCTTCATCA TCAAGTGCAG CCGTGAGACT
    GAGAAGAAAT ATCCCCAGCC GAAGGGGCAG AAGAAGAAGA AGATTGTCAA GTACGGCATG
    GGCGGTCTCA TCATCCTGTT CCTCGTGGCT ATCATCTGGT TCCCGTTGCT TTTCATGTCC
    CTGGTACGCT CTGTGGTTGG TGTCGTCAAC CAGCCCATCG ATGTTACTGT CACCCTTAAG
    CTGGGTGGCT ATGAGCCCCT CTTTACCATG AGTGCCCAGC AGCCGTCCAT CGTGCCGTTC
    ACACAGCAGG CCTATGAGGA GCTGTCCAGA CAGTTTGACC CTAACCCGTT GGCCATGCAG
    TTCATCAGCC AGTACAGCCC CGAGGACATC GTCACGGCAC AGATCGAGGG CAGTTCAGGG
    GCACTGTGGC GCATCAGCCC ACCCAGCCGG GCCCAGATGA AGCGGGAGCT ATACAATGGT
    ACAGCTGACA TCACCCTGCG CTTCACCTGG AACTTCCAGA GGGACCTGGC CAAGGGCGGC
    ACCGTGGAGT ACACCAATGA GAAGCACACC CTGGACTGGG CCCCAAATAG CACTGAGCGG
    CGGCAGCTGG CCAGCCTGCT TGAGGGCACC TCGGACCAGT CCGTGGTCAT CCCCCACCTC
    TTTCCCAAGT ACATCCGTGC CCCTAATGGG CCTGAAGCCA ACCCAGTGAA GCAGCTGCAG
    CCCAACGAGG AGGCCGACTA CCTAGGCGTG CGCATCCAGC TGCGGAGGGA GCGCGTGGGC
    TCAGGGGCCG CTGGCTTCCT TGAGTGGTGG GTCATCGAGC TGCAGGATTG CCAGGCCGAG
    TGCAACCTGC TGCCCATGGT CATCTTCAGT GACAAGGTCA GCCCACCCAG CCTTGGCTTC
    CTGGCTGGCT ACGGGATCAT GGGGCTCTAT GTGTCCATCG TGCTGGTTAT CGGCAAGTTT
    GTACGCGGCT TCTTCAGTGA GATCTCCCAC TCCATCATGT TTGAGGAGCT GCCGTGTGTG
    GACCGCATCC TCAAGCTCTG CCAGGACATC TTCCTGGTGC GGGAGACGCG GGAGCTGGAG
    CTGGAAGAGG AGCTTTATGC CAAGCTTATC TTCCTGTACC GCTCGCCTGA GACGATGATC
    AAGTGGACCC GTGAGAAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620633.1
    CDS269..7732
    Translation

    Target ORF information:

    RefSeq Version XM_005620576.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGAGCGGC ACGTGCTCGG CGCCGTCCTC TACTGGCTGC TGCTGCCCTT GGCACTCCTG 
    GCTGCCTGCC TGTTCCGCTA TAACGCGCTG TCTCTGGTCT ATCTGCTCTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTGTCGGCGC GGCTTCCCAG GCCACACTCG TCGCCTGCTC
    CGGGCCCTGC TGGTTTTCAG CCTCATCTTC CTGGCAGCTC ACCTGACCTT CCAGATATGC
    CTGCACACTG TGCCCCACCT GTACCAGCTT CTGGAGCCCA GCTGTGGCCC CTGGGAGACC
    CTCTCTCGGC ACATTGGGGT CACAAGGCTG GACCTGAAGG ACATCCCCAC TGCTGTCCGG
    CTGGTGGCCC CCGACCTTGG TGTCCTGGTG GTCTGCGCTG TGTGCCTCGG CCTCTGCGGG
    CGCCTCACAC AGGAAGCCCC ACGGAGCCAG CGTGCCCAGG AGCTGGACGA TGATGATGAT
    GGGGAAGTGG ACACCGGCTC CCCTGTGGGG CTTCAGGGAG CCCCCGTGCA GGCCCCCACG
    CGGAGGTCAC GGTTGGCCAC CCGGTTCCAG ATCACAGCCC ACTGGCTGTT GGTGGCCGCG
    GGAAGGACCC TGGCTATTGT GCTGCTCGCA TTGGCAGGCA TCGCCCACCC CTCTGCCTTC
    TCCAGCGTCT ACTTCCTAGT CTTCCTGGCC ACCTGCACCT GGTGGGCATG CCACTTTCCC
    ATCAGCCTCC TGGGCTTCAG CACGCTCTGC GTCACAGTGG GCTGCTTCGG TGCCGGCCAT
    CTCCTCTGCA TCTACTGCTA CCAGACGCCC TTTGCCCAGA CCGTGCTCCC TCCTGCTGGA
    ATCTGGGCCA GGGTGTTTGG TCTCAAGGCC TTGGTGACCC ACGGCAACTG CTCCAGCCCC
    AACGTGCTGG TCTTCAGTAC CAGCCACGAC TGGCCCATCT ACGTGAGCCC CGGCATCTTG
    CTGCTTCTCT ACTACACGGT GACCTCACTC CTGAAGCTCC GCACTCGCCA GCCGTTGGAC
    CAGAGGAAGG AAGTGGCCAG GAACGACGAG GAGCAGGAGG TAGAGCTGAC CCAGGTGGAC
    CAGTGGCTCC AGGACCAGGC CAGAGCTGCA GCCACCAAGG AGGAGGGGGC TGCCCAGCAC
    ATGCTGCCCA CAACCGTGGG GTCTGACTGG GAGACCGACA GCTGTACGGT GCATGACCTG
    ACTGGCCACA CCCCCGTCCG GCAGCGTCCA GTGCACCCCA GGCTGGCTGA GCCTCAAGAG
    ACATCTACCC TCCACAGTCT GGGTCATCTC ATTATGGACC AGAGCTACGT GTGTGCCCTC
    ATTGCCATGA TGGTATGGAG CATCACCTAC CATAGTTGGC TGACCTTCGT GCTGCTGCTC
    TGGGCCTGCC TCATCTGGAC GGTGCGCAGC CGCCACCAGA TGGCCATGCT CTGCTCACCC
    TTCATCCTGC TCTACGGGCT GGCTCTGTGT GGCCTGCGCT ATGTGTGGGC CATGGACCTG
    CGTCCCGAGC TGCCTACCAC CCTGGGCCCC GTCAGCCTGC GTCAGCTGGG GCTGGAGCAT
    ACCCGCTACC CTTGCCTGGA CCTTGGCGCC ATGTTGCTCT ACACCCTGAC CTTCTGGCTG
    CTGCTGCGCC GGTTTGTCAA GGAGAAGCTG CTGAAGAAGG GGAAGGTACC TGCTGCCCTG
    ACAGAGGTCA CTGTGGCTGA CACAGAGCCA ACACGGGCAC GGACGCTGCT GCAGAGCCTG
    GGGGAGCTGG TCACAGGCCT GTACGCCAAG TACTGGATCT ACGTGTGTGC CGGCATGTTC
    ATTGTGGTCA GCTTCGCCGG CCGCCTCGTC GTCTACAAGA TCGTGTACAT GCTCCTTTTC
    CTGCTCTGCC TCACCCTCTT CCAGGTCTAC TACAGCCTGT GGAGGAAGCT GCTCAAGGTG
    TTCTGGTGGC TGGTGGTGGC CTATACCATG CTGGTGCTCA TCGCCGTCTA CACTTTCCAG
    TTCCAGGACT TCCCCATGTA CTGGCGCAAC CTGACGGGCC TCACTGACGA ACAGCTGGGG
    GACCTGGGCC TGGAGCAGTT CAGCGTGTCG GAGCTTTTCT CCAGCATCCT GATCCCGGGC
    TTCTTCCTGT TGGCCTGCAT CCTGCAGCTG CACTATTTCC ACCGGCCGTT CATGCAGCTC
    ACGGACCTGG AGCATGTGCC CCCGCCTGGT GCCTGCCCCC CACGGTGGGC CCACAGGCAG
    GATGTGGTCA GTGGGACCCC GCTGCTGCAG GAGGAGGAGG ACGGGCTGAG CACCGCCTGC
    CCCCACCAGG CCATGCAGGT CACAGAAGCC AACAAGTGGG GCCTGGTGGC GGAACGGCTG
    CTGGACCTGG CTTCCGGCTT CTCGGACGTC CTCACCCGTG TGCAGGTGCT GCTGCGGCGC
    CTTCTAGAGT TGCACATCCT CAAGCTGGTG GCCCTGTACA CTGTGTGGGT GGCCCTGAAG
    GAGGTGTCCG TGATGAACCT GCTGCTGGTC GTGCTGTGGG CCTTCGCGCT GCCCTACCCC
    CGCTTCCGGC CCATGGCCTC CTGCCTGTCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTCGTAAGC CCGCACGAGT ACTCCAGCAA CTGCACCGAG
    CCCCTCCCCA ACAGCACCAA CCTGCAGAAG ATGGAGATCA AGCAGTCCTT GCTATACCGT
    GGGCCCGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCAAA CCTAGGCTAC
    ATCCAGAACC ACCTGCAGGT CCTGTTGCTG CTGGTGTTCG AGGCCATCGT CTACCGGCGT
    CAGGAGCACC ACCGTCGGCA GCACCAGCTG GCCCCACTGC CCGCCCAGGC CGTCTGTGCC
    GACGGCACCC GCCAGCGGCT GGACCAGGAC CTGCTCAGCT GCCTCAAGTA CTTTGTCAAC
    TTCTTTTTCT ACAAATTTGG GCTAGAGATA TGCTTCCTGA TGGCCGTGAA CGTGATTGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTCACACGG
    CGGCGCCGTG CAGCCATCGC CCGCCTCTGG CCCAACTACT GTCTCTTCCT GTCCCTGTTC
    TTGCTGTACC AGTACCTGCT GTGCCTGGGC ATCCCCCCGG CCCTGTGCAT CGACTACCCG
    TGGCGCTGGA GCCAGGCCAT CCCCATGAAC TCAGCACTCA TCAAGTGGCT GTACCTGCCC
    GACTTCTTCA GAACCCCCAA TTCCACCAAC CTCATCAGCG ACTTCCTGCT CCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCT GAGCGCACGG AGGAATGGCA GCACATGGCC
    GGCGTCAACA CTGACCGCCT GGAGCTGCCA TTGGGTGAGC CCAACGCCGT GCCCAACTTC
    ATCTACTGCA GGTCCTACCT CGACATGCTG AAGGTGGCTG TCTTCCGCTA CCTCTTCTGG
    CTGGTGCTGG TAGTGGTATT TGTCACGGGA GCCACCCGCG TCAGCGTCTT CGGGCTGGGC
    TACCTGTTGG CCTGTTTCTA TCTGCTGCTC TTCGGCACCA GCCTATTACA GAAGCACACA
    CGCACCCGCC TCGTGCTGTG GGACTGCCTC ATTCTCTACA ACGTCACTGT CATCATCTCT
    AAGAACATGC TCTCGCTCCT GTCCTGCGTT TTTGTGGAGC AAATGCAGAG CAACTTCTGC
    TGGGTTATCC AGCTTTTCAG CCTCGTGTGC ACCGTCAAAG GCTACTATGA TCCCAAGGAG
    ATGCTGAGCA GGGACCGGGA TTGCCTGCTG CCCGTGGAAG AGGCCGGTGT GCTCTGGGAC
    AGCATCTGCT TCCTGTTCCT TCTGCTGCAG CGGCGCGTCT TCCTCAGCCG CTACTTCCTG
    CATGTCAGCG CCGAGCTCCA GGCCACCGCC TTGCAGGCCT CCAGGGGCTT CGCGCTGTAC
    AATGCTGCCA ACTTCAAAAG CATCGATCTC CACCGTAAGG CCGAGGAGAA GTCCCTGGCC
    CAGCTGAAAA GACAGATGGA GCGCATCCGT GCCAAGCAAG AGAAGCACAG GCAGAGCCGA
    GCGGGCCGCA GCCGACCCCA GGACACCCCA GACCCCACCC AGGAGCCAGG GCCTGGCAGT
    CCAGGGGGCT CCTCCCTGCC ACGGAGACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTCATCCACT CTGGGGACTA CTTCCTATTT GAGTCGGATA GTGAGGAGGA GGAGGAGGCC
    CTGCCCGAGG ACCCCAGGCC GTCAGCACAG AGTGCCTTTC AGATGGCGTA CCAGGCATGG
    GTGACCAATG CGCAGACAGT GCTGAGGCAG CAGCGGGAGG AGCAGGCGAG GCAGGACCAG
    ACAGGGCAGT TGCCCACGGG AGGCGGCCCG GGACAGGAGG CAGAGCCAGC GGATGGCCCA
    GACGAGGTGG TGGCTGGCCG CAGCCACGTG ATGCAGCGAG TGCTGAGCAC TGTGCAGTTT
    CTGTGGGTGC TGGGCCAAGC GCTGGTAGAT GGGCTGACAG ACTGGCTGCA CACCTTCACA
    CGGCACCACC GCGCCATGAG TGACGTGCTG CGCGCCGAGC GCTACCTGTA CACACAGGCG
    CTGGTTGAGG GCAGAGAGGT GCACCGGAGC TTGCTGGACC AGCTCTACAC TGGTGAAGCC
    GAGGCTGCCC CCGCAGTCCC CTTGGGGGTC CGAGATGCAC CAAGTACAGC ATCAAGCAGC
    AGCAGCGAGG AGGTGGCCAC TGAGCCTGGG GCTTCCCTAC ACGGCTCTCG GGAGCTTCCT
    GCTGGCACTC CTACCCGGAT GCGCACAGCC AGTGAGCTGC TCCTCAGCAG GCACCTCCGC
    ATCCAGGAGC TGGAGGAGGC CGAGCTGTTT GAAGCAGGGC AGGGCCGGGC ACTGCGGCTG
    CTGCAGGCCG CATACCAGTG TGTGGCTGCC CACTCAGAAC TGCTCTGCTA CTTCATCATC
    ATCCTCAACC ACATGGTCAC TGCCTCAGCC ACATCCCTGG TGCTGCCTGT GCTCGTCTTC
    CTGTGGGCCA TGCTGTCCAT CCCCAGGCCC AGCAAGCGCT TCTGGATGAC GGCCATCATC
    TTCACCGAGG TGTCTGTGGT CACCAAGTAC CTGTTCCAGT TTGGCTTCTT CCCCTGGAAC
    ACACACACAG TGCTCCGGCG CTACGAGAAC AAGCCGTACT TCCCACCGCG CATCCTGGGT
    GTGGAGAAGA CAGACAGCTA CATAAAGTAT GACCTGGTGC AGCTCATGGC CCTCTTCTTC
    CACCGCTCCC AGCTGCTGTG CTATGGTCTC TGGGATCATG AGGAGGACCC GCTGTCCAAG
    GAGCATGACC GTGGAAGTGA GAAGAAAGGG ACCGAGGAGG AACAGGCACC ACTGGAACCC
    CAGACTGAGG AAGGCACAGG GCCCCAGGGC GAGCCAGTGG TGGCCGGGGC CCTCACCCAG
    GACCACATCC AGGCAGAAGC AGGGGATGGG CCCCCAGAGC CTCCTGTGGA GCTCAAGCCC
    CGAGACATAA AGCGTATCAG CCTGCGTTTC AGGAAGAGGC GGAGGGAGAC CACAGAGCCT
    GTACAACCCA CAGCCACTGA AGGTGTGGTG GTGGCCTCCC GCAGAGAGAA GTGGAGTCGC
    CCCCGGGAAA GAATGACAGC CATGGGGCTC CGACTGCAGA CCTTCTGCCT GGCCGTGGCC
    CAGAGCATGT ACCGGCCCCT GCGGCGCTTC TTCGATGACA TCTTGCACAC CAAGTACCGT
    GCAGCCACCG ATGTCTACGC GCTCATGTTT CTGGCTGATG TGGTCGACTT CATCATCATC
    ATCTTCGGCT TCTGGGCCTT TGGGAAACAC TCGGCAGCCA CAGACATCAC ATCCTCGCTG
    TCGGACGACC AGGTGCCGGA GGCCTTCCTG GTCATGCTGC TCATCCAGTT CAGCACCATG
    GTCATTGACC GTGCCCTCTA CCTCCGCAAG ACCGTGCTGG GCAAGCTGGC CTTCCAGGTT
    GTCCTGGTGC TGGCCATCCA CCTCTGGATG TTCTTTATTC TGCCCGCTGT CACCGAGAGG
    ATGTTCAACC AGAACGCGGT GGCTCAGCTG TGGTACTTCG TGAAGTGCAT CTACTTCTCC
    CTGTCTGCCT ACCAGATCCG CTGTGGCTAT CCGACCCGTA TCCTTGGCAA CTTCCTCACC
    AAGAAGTACA ATCACCTGAA CCTCTTCCTC TTCCAGGGGT TCCGGCTTGT GCCATTCCTG
    GTGGAGCTGC GGGCCGTGAT GGACTGGGTA TGGACAGACA CCACGCTGTC CCTGTCCAGC
    TGGATGTGTG TGGAGGACAT CTATGCCAAC ATCTTCATCA TCAAGTGCAG CCGTGAGACT
    GAGAAGAAAT ATCCCCAGCC GAAGGGGCAG AAGAAGAAGA AGATTGTCAA GTACGGCATG
    GGCGGTCTCA TCATCCTGTT CCTCGTGGCT ATCATCTGGT TCCCGTTGCT TTTCATGTCC
    CTGGTACGCT CTGTGGTTGG TGTCGTCAAC CAGCCCATCG ATGTTACTGT CACCCTTAAG
    CTGGGTGGCT ATGAGCCCCT CTTTACCATG AGTGCCCAGC AGCCGTCCAT CGTGCCGTTC
    ACACAGCAGG CCTATGAGGA GCTGTCCAGA CAGTTTGACC CTAACCCGTT GGCCATGCAG
    TTCATCAGCC AGTACAGCCC CGAGGACATC GTCACGGCAC AGATCGAGGG CAGTTCAGGG
    GCACTGTGGC GCATCAGCCC ACCCAGCCGG GCCCAGATGA AGCGGGAGCT ATACAATGGT
    ACAGCTGACA TCACCCTGCG CTTCACCTGG AACTTCCAGA GGGACCTGGC CAAGGGCGGC
    ACCGTGGAGT ACACCAATGA GAAGCACACC CTGGACTGGG CCCCAAATAG CACTGAGCGG
    CGGCAGCTGG CCAGCCTGCT TGAGGGCACC TCGGACCAGT CCGTGGTCAT CCCCCACCTC
    TTTCCCAAGT ACATCCGTGC CCCTAATGGG CCTGAAGCCA ACCCAGTGAA GCAGCTGCAG
    CCCAACGAGG AGGCCGACTA CCTAGGCGTG CGCATCCAGC TGCGGAGGGA GCGCGTGGGC
    TCAGGGGCCG CTGGCTTCCT TGAGTGGTGG GTCATCGAGC TGCAGGATTG CCAGGCCGAG
    TGCAACCTGC TGCCCATGGT CATCTTCAGT GACAAGGTCA GCCCACCCAG CCTTGGCTTC
    CTGGCTGGCT ACGGGATCAT GGGGCTCTAT GTGTCCATCG TGCTGGTTAT CGGCAAGTTT
    GTACGCGGCT TCTTCAGTGA GATCTCCCAC TCCATCATGT TTGAGGAGCT GCCGTGTGTG
    GACCGCATCC TCAAGCTCTG CCAGGACATC TTCCTGGTGC GGGAGACGCG GGAGCTGGAG
    CTGGAAGAGG AGCTTTATGC CAAGCTTATC TTCCTGTACC GCTCGCCTGA GACGATGATC
    AAGTGGACCC GTGAGAAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.