PFAS cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PFAS
Entrez Gene ID	489490
Full Name	phosphoribosylformylglycinamidine synthase
General protein information	Preferred Names phosphoribosylformylglycinamidine synthase Names phosphoribosylformylglycinamidine synthase phosphoribosylformylglycinamidine synthase (FGAR amidotransferase)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_845074.5	XP_850167.2	phosphoribosylformylglycinamidine synthase
XM_005620022.2	XP_005620079.1	phosphoribosylformylglycinamidine synthase
XM_014113585.1	XP_013969060.1	phosphoribosylformylglycinamidine synthase
XM_014113586.1	XP_013969061.1	phosphoribosylformylglycinamidine synthase
XM_014113585.2	XP_013969060.1	phosphoribosylformylglycinamidine synthase
XM_014113586.2	XP_013969061.1	phosphoribosylformylglycinamidine synthase
XM_845074.4	XP_850167.2	phosphoribosylformylglycinamidine synthase
XM_022418557.1	XP_022274265.1	phosphoribosylformylglycinamidine synthase

Pathways from BioSystems

Homologies

	PFAS	XP_001112405.1
Pan troglodytes (chimpanzee)	PFAS	XP_511854.3
Bos taurus (cattle)	PFAS	NP_001243493.1
Mus musculus (house mouse)	Pfas	NP_001152991.1
Drosophila melanogaster (fruit fly)	ade2	NP_723146.1
Caenorhabditis elegans (roundworm)	CELE_F10F2.2	NP_497942.2
Homo sapiens (human)	PFAS	NP_036525.1
Canis lupus familiaris (dog)	PFAS	XP_005620078.1
Rattus norvegicus (Norway rat)	Pfas	NP_001099261.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002091	XP_320948.2
Arabidopsis thaliana (thale cress)	PUR4	NP_177566.3
Danio rerio (zebrafish)	pfas	NP_001038667.1
Oryza sativa (rice)	Os01g0888500	NP_001045039.1
Xenopus tropicalis (tropical clawed frog)	pfas	NP_001135692.2

Gene Ontology

The following PFAS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFAS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe06286	XM_014113585.2 Latest version!	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe06286	XM_022418557.1 Latest version!	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe06286	XM_014113586.2 Latest version!	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe06286	XM_845074.5 Latest version!	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe06286	XM_005620022.2 Latest version!	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OCe06286	XM_014113585.1	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe06286	XM_014113586.1	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe06286	XM_845074.4	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFAS ( XM_845074.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_850167.2 Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

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CloneID	OCe06286
Clone ID Related Accession (Same CDS sequence)	XM_845074.5 , XM_005620022.2 , XM_014113585.1 , XM_014113586.1 , XM_014113585.2 , XM_014113586.2 , XM_845074.4 , XM_022418557.1
Accession Version	XM_845074.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	phosphoribosylformylglycinamidine synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_845074.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_850167.2
CDS	244..4269
Translation	MTSVLHFYVHPSGHERAASEHTQRKLQGKLPALQSVKTELCYNVNWTAKSPPNAEEMKKLMWLFGCPLLLGDVAQESWLHPDSSDLLLEVGPRLNFSTPASTNVVSVCWAAGLGAVDRVETTRRYLLSFAHPPSTEMETIALATLHDRMTEQHFPHPIQSFSLGSISTPLNGHINILAEGRCALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHMDGQELAHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVQFLRPEDPTQPSCFQPHRALRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPENFARPLEVAIEASNGASDYGNKFGEPVLAGFTRSFGLQLPDGQRREWIKPIMFSGGIGSMEAEHVRKEPPEPGMDIVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAARGNPICSLHDQGAGGNGNVLKELSDPAGAVIYTSRFQLGDPTLNALEIWGAEYQESNALLLRPLDRDFLSRVSARERCPTCFVGTITGDKRIVLVDDREYPVGRNGHEDASLSSPPTPVDLELDWVLGKMPRKEFFLKRSHPVLKPLALPPGLRVRQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLHTPLADVAVVALSHQELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCPDITATVTPDLKHPGGRGHLLYVSLSPGQHRLGGTALAQCFSQLGEQPPNLDLPENLVRAFSVTQGLLRDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVHVPAPGVDALSVLFAEEPGLVLEVQESDLAQVLKRYRVAGLHCMELGPTGDAGPHALVRVSVNGAVVLEEAVGQLRAVWEETSFQLDRLQAEPRCVAEEEQGLRERMGPRYCLPAAFPKASVPREPGGPVPRVAILREEGSNGDREMADAFHLAGFEVWDVTTQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPVARAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGSPSEEAKETKETSLDSWPAPPGLVLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQIEARGLAPLHWADDNGKPTEQYPMNPNGSPGGVAGVCSLDGRHLAVMPHPERAVRPWQWAWRPPLFDTVTTSPWLQLFINARDWTRKGGC

Target ORF information:

RefSeq Version	XM_845074.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_845074.5

ORF Insert Sequence:

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_005620022.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620079.1 Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe06286
Clone ID Related Accession (Same CDS sequence)	XM_845074.5 , XM_005620022.2 , XM_014113585.1 , XM_014113586.1 , XM_014113585.2 , XM_014113586.2 , XM_845074.4 , XM_022418557.1
Accession Version	XM_005620022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	phosphoribosylformylglycinamidine synthase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620079.1
CDS	354..4379
Translation	MTSVLHFYVHPSGHERAASEHTQRKLQGKLPALQSVKTELCYNVNWTAKSPPNAEEMKKLMWLFGCPLLLGDVAQESWLHPDSSDLLLEVGPRLNFSTPASTNVVSVCWAAGLGAVDRVETTRRYLLSFAHPPSTEMETIALATLHDRMTEQHFPHPIQSFSLGSISTPLNGHINILAEGRCALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHMDGQELAHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVQFLRPEDPTQPSCFQPHRALRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPENFARPLEVAIEASNGASDYGNKFGEPVLAGFTRSFGLQLPDGQRREWIKPIMFSGGIGSMEAEHVRKEPPEPGMDIVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAARGNPICSLHDQGAGGNGNVLKELSDPAGAVIYTSRFQLGDPTLNALEIWGAEYQESNALLLRPLDRDFLSRVSARERCPTCFVGTITGDKRIVLVDDREYPVGRNGHEDASLSSPPTPVDLELDWVLGKMPRKEFFLKRSHPVLKPLALPPGLRVRQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLHTPLADVAVVALSHQELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCPDITATVTPDLKHPGGRGHLLYVSLSPGQHRLGGTALAQCFSQLGEQPPNLDLPENLVRAFSVTQGLLRDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVHVPAPGVDALSVLFAEEPGLVLEVQESDLAQVLKRYRVAGLHCMELGPTGDAGPHALVRVSVNGAVVLEEAVGQLRAVWEETSFQLDRLQAEPRCVAEEEQGLRERMGPRYCLPAAFPKASVPREPGGPVPRVAILREEGSNGDREMADAFHLAGFEVWDVTTQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPVARAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGSPSEEAKETKETSLDSWPAPPGLVLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQIEARGLAPLHWADDNGKPTEQYPMNPNGSPGGVAGVCSLDGRHLAVMPHPERAVRPWQWAWRPPLFDTVTTSPWLQLFINARDWTRKGGC

Target ORF information:

RefSeq Version	XM_005620022.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620022.2

ORF Insert Sequence:

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_014113585.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969060.1 Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

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CloneID	OCe06286
Clone ID Related Accession (Same CDS sequence)	XM_845074.5 , XM_005620022.2 , XM_014113585.1 , XM_014113586.1 , XM_014113585.2 , XM_014113586.2 , XM_845074.4 , XM_022418557.1
Accession Version	XM_014113585.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	phosphoribosylformylglycinamidine synthase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969060.1
CDS	359..4384
Translation	MTSVLHFYVHPSGHERAASEHTQRKLQGKLPALQSVKTELCYNVNWTAKSPPNAEEMKKLMWLFGCPLLLGDVAQESWLHPDSSDLLLEVGPRLNFSTPASTNVVSVCWAAGLGAVDRVETTRRYLLSFAHPPSTEMETIALATLHDRMTEQHFPHPIQSFSLGSISTPLNGHINILAEGRCALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHMDGQELAHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVQFLRPEDPTQPSCFQPHRALRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPENFARPLEVAIEASNGASDYGNKFGEPVLAGFTRSFGLQLPDGQRREWIKPIMFSGGIGSMEAEHVRKEPPEPGMDIVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAARGNPICSLHDQGAGGNGNVLKELSDPAGAVIYTSRFQLGDPTLNALEIWGAEYQESNALLLRPLDRDFLSRVSARERCPTCFVGTITGDKRIVLVDDREYPVGRNGHEDASLSSPPTPVDLELDWVLGKMPRKEFFLKRSHPVLKPLALPPGLRVRQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLHTPLADVAVVALSHQELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCPDITATVTPDLKHPGGRGHLLYVSLSPGQHRLGGTALAQCFSQLGEQPPNLDLPENLVRAFSVTQGLLRDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVHVPAPGVDALSVLFAEEPGLVLEVQESDLAQVLKRYRVAGLHCMELGPTGDAGPHALVRVSVNGAVVLEEAVGQLRAVWEETSFQLDRLQAEPRCVAEEEQGLRERMGPRYCLPAAFPKASVPREPGGPVPRVAILREEGSNGDREMADAFHLAGFEVWDVTTQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPVARAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGSPSEEAKETKETSLDSWPAPPGLVLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQIEARGLAPLHWADDNGKPTEQYPMNPNGSPGGVAGVCSLDGRHLAVMPHPERAVRPWQWAWRPPLFDTVTTSPWLQLFINARDWTRKGGC

Target ORF information:

RefSeq Version	XM_014113585.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113585.1

ORF Insert Sequence:

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_014113586.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969061.1 Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X4, mRNA.

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CloneID	OCe06286
Clone ID Related Accession (Same CDS sequence)	XM_845074.5 , XM_005620022.2 , XM_014113585.1 , XM_014113586.1 , XM_014113585.2 , XM_014113586.2 , XM_845074.4 , XM_022418557.1
Accession Version	XM_014113586.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	phosphoribosylformylglycinamidine synthase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969061.1
CDS	121..4146
Translation	MTSVLHFYVHPSGHERAASEHTQRKLQGKLPALQSVKTELCYNVNWTAKSPPNAEEMKKLMWLFGCPLLLGDVAQESWLHPDSSDLLLEVGPRLNFSTPASTNVVSVCWAAGLGAVDRVETTRRYLLSFAHPPSTEMETIALATLHDRMTEQHFPHPIQSFSLGSISTPLNGHINILAEGRCALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHMDGQELAHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVQFLRPEDPTQPSCFQPHRALRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPENFARPLEVAIEASNGASDYGNKFGEPVLAGFTRSFGLQLPDGQRREWIKPIMFSGGIGSMEAEHVRKEPPEPGMDIVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAARGNPICSLHDQGAGGNGNVLKELSDPAGAVIYTSRFQLGDPTLNALEIWGAEYQESNALLLRPLDRDFLSRVSARERCPTCFVGTITGDKRIVLVDDREYPVGRNGHEDASLSSPPTPVDLELDWVLGKMPRKEFFLKRSHPVLKPLALPPGLRVRQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLHTPLADVAVVALSHQELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCPDITATVTPDLKHPGGRGHLLYVSLSPGQHRLGGTALAQCFSQLGEQPPNLDLPENLVRAFSVTQGLLRDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVHVPAPGVDALSVLFAEEPGLVLEVQESDLAQVLKRYRVAGLHCMELGPTGDAGPHALVRVSVNGAVVLEEAVGQLRAVWEETSFQLDRLQAEPRCVAEEEQGLRERMGPRYCLPAAFPKASVPREPGGPVPRVAILREEGSNGDREMADAFHLAGFEVWDVTTQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPVARAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGSPSEEAKETKETSLDSWPAPPGLVLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQIEARGLAPLHWADDNGKPTEQYPMNPNGSPGGVAGVCSLDGRHLAVMPHPERAVRPWQWAWRPPLFDTVTTSPWLQLFINARDWTRKGGC

Target ORF information:

RefSeq Version	XM_014113586.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113586.1

ORF Insert Sequence:

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_014113585.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969060.1 Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe06286
Clone ID Related Accession (Same CDS sequence)	XM_845074.5 , XM_005620022.2 , XM_014113585.1 , XM_014113586.1 , XM_014113585.2 , XM_014113586.2 , XM_845074.4 , XM_022418557.1
Accession Version	XM_014113585.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	phosphoribosylformylglycinamidine synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969060.1
CDS	168..4193
Translation	MTSVLHFYVHPSGHERAASEHTQRKLQGKLPALQSVKTELCYNVNWTAKSPPNAEEMKKLMWLFGCPLLLGDVAQESWLHPDSSDLLLEVGPRLNFSTPASTNVVSVCWAAGLGAVDRVETTRRYLLSFAHPPSTEMETIALATLHDRMTEQHFPHPIQSFSLGSISTPLNGHINILAEGRCALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHMDGQELAHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVQFLRPEDPTQPSCFQPHRALRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPENFARPLEVAIEASNGASDYGNKFGEPVLAGFTRSFGLQLPDGQRREWIKPIMFSGGIGSMEAEHVRKEPPEPGMDIVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAARGNPICSLHDQGAGGNGNVLKELSDPAGAVIYTSRFQLGDPTLNALEIWGAEYQESNALLLRPLDRDFLSRVSARERCPTCFVGTITGDKRIVLVDDREYPVGRNGHEDASLSSPPTPVDLELDWVLGKMPRKEFFLKRSHPVLKPLALPPGLRVRQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLHTPLADVAVVALSHQELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCPDITATVTPDLKHPGGRGHLLYVSLSPGQHRLGGTALAQCFSQLGEQPPNLDLPENLVRAFSVTQGLLRDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVHVPAPGVDALSVLFAEEPGLVLEVQESDLAQVLKRYRVAGLHCMELGPTGDAGPHALVRVSVNGAVVLEEAVGQLRAVWEETSFQLDRLQAEPRCVAEEEQGLRERMGPRYCLPAAFPKASVPREPGGPVPRVAILREEGSNGDREMADAFHLAGFEVWDVTTQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPVARAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGSPSEEAKETKETSLDSWPAPPGLVLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQIEARGLAPLHWADDNGKPTEQYPMNPNGSPGGVAGVCSLDGRHLAVMPHPERAVRPWQWAWRPPLFDTVTTSPWLQLFINARDWTRKGGC

Target ORF information:

RefSeq Version	XM_014113585.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113585.2

ORF Insert Sequence:

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_014113586.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969061.1 Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe06286
Clone ID Related Accession (Same CDS sequence)	XM_845074.5 , XM_005620022.2 , XM_014113585.1 , XM_014113586.1 , XM_014113585.2 , XM_014113586.2 , XM_845074.4 , XM_022418557.1
Accession Version	XM_014113586.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	phosphoribosylformylglycinamidine synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113586.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969061.1
CDS	173..4198
Translation	MTSVLHFYVHPSGHERAASEHTQRKLQGKLPALQSVKTELCYNVNWTAKSPPNAEEMKKLMWLFGCPLLLGDVAQESWLHPDSSDLLLEVGPRLNFSTPASTNVVSVCWAAGLGAVDRVETTRRYLLSFAHPPSTEMETIALATLHDRMTEQHFPHPIQSFSLGSISTPLNGHINILAEGRCALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHMDGQELAHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVQFLRPEDPTQPSCFQPHRALRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPENFARPLEVAIEASNGASDYGNKFGEPVLAGFTRSFGLQLPDGQRREWIKPIMFSGGIGSMEAEHVRKEPPEPGMDIVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAARGNPICSLHDQGAGGNGNVLKELSDPAGAVIYTSRFQLGDPTLNALEIWGAEYQESNALLLRPLDRDFLSRVSARERCPTCFVGTITGDKRIVLVDDREYPVGRNGHEDASLSSPPTPVDLELDWVLGKMPRKEFFLKRSHPVLKPLALPPGLRVRQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLHTPLADVAVVALSHQELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCPDITATVTPDLKHPGGRGHLLYVSLSPGQHRLGGTALAQCFSQLGEQPPNLDLPENLVRAFSVTQGLLRDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVHVPAPGVDALSVLFAEEPGLVLEVQESDLAQVLKRYRVAGLHCMELGPTGDAGPHALVRVSVNGAVVLEEAVGQLRAVWEETSFQLDRLQAEPRCVAEEEQGLRERMGPRYCLPAAFPKASVPREPGGPVPRVAILREEGSNGDREMADAFHLAGFEVWDVTTQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPVARAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGSPSEEAKETKETSLDSWPAPPGLVLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQIEARGLAPLHWADDNGKPTEQYPMNPNGSPGGVAGVCSLDGRHLAVMPHPERAVRPWQWAWRPPLFDTVTTSPWLQLFINARDWTRKGGC

Target ORF information:

RefSeq Version	XM_014113586.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113586.2

ORF Insert Sequence:

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_845074.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_850167.2 Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

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CloneID	OCe06286
Clone ID Related Accession (Same CDS sequence)	XM_845074.5 , XM_005620022.2 , XM_014113585.1 , XM_014113586.1 , XM_014113585.2 , XM_014113586.2 , XM_845074.4 , XM_022418557.1
Accession Version	XM_845074.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	phosphoribosylformylglycinamidine synthase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_845074.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_850167.2
CDS	193..4218
Translation	MTSVLHFYVHPSGHERAASEHTQRKLQGKLPALQSVKTELCYNVNWTAKSPPNAEEMKKLMWLFGCPLLLGDVAQESWLHPDSSDLLLEVGPRLNFSTPASTNVVSVCWAAGLGAVDRVETTRRYLLSFAHPPSTEMETIALATLHDRMTEQHFPHPIQSFSLGSISTPLNGHINILAEGRCALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHMDGQELAHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVQFLRPEDPTQPSCFQPHRALRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPENFARPLEVAIEASNGASDYGNKFGEPVLAGFTRSFGLQLPDGQRREWIKPIMFSGGIGSMEAEHVRKEPPEPGMDIVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAARGNPICSLHDQGAGGNGNVLKELSDPAGAVIYTSRFQLGDPTLNALEIWGAEYQESNALLLRPLDRDFLSRVSARERCPTCFVGTITGDKRIVLVDDREYPVGRNGHEDASLSSPPTPVDLELDWVLGKMPRKEFFLKRSHPVLKPLALPPGLRVRQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLHTPLADVAVVALSHQELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCPDITATVTPDLKHPGGRGHLLYVSLSPGQHRLGGTALAQCFSQLGEQPPNLDLPENLVRAFSVTQGLLRDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVHVPAPGVDALSVLFAEEPGLVLEVQESDLAQVLKRYRVAGLHCMELGPTGDAGPHALVRVSVNGAVVLEEAVGQLRAVWEETSFQLDRLQAEPRCVAEEEQGLRERMGPRYCLPAAFPKASVPREPGGPVPRVAILREEGSNGDREMADAFHLAGFEVWDVTTQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPVARAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGSPSEEAKETKETSLDSWPAPPGLVLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQIEARGLAPLHWADDNGKPTEQYPMNPNGSPGGVAGVCSLDGRHLAVMPHPERAVRPWQWAWRPPLFDTVTTSPWLQLFINARDWTRKGGC

Target ORF information:

RefSeq Version	XM_845074.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_845074.4

ORF Insert Sequence:

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_022418557.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022274265.1 Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

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CloneID	OCe06286
Clone ID Related Accession (Same CDS sequence)	XM_845074.5 , XM_005620022.2 , XM_014113585.1 , XM_014113586.1 , XM_014113585.2 , XM_014113586.2 , XM_845074.4 , XM_022418557.1
Accession Version	XM_022418557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	phosphoribosylformylglycinamidine synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022274265.1
CDS	166..4191
Translation	MTSVLHFYVHPSGHERAASEHTQRKLQGKLPALQSVKTELCYNVNWTAKSPPNAEEMKKLMWLFGCPLLLGDVAQESWLHPDSSDLLLEVGPRLNFSTPASTNVVSVCWAAGLGAVDRVETTRRYLLSFAHPPSTEMETIALATLHDRMTEQHFPHPIQSFSLGSISTPLNGHINILAEGRCALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHMDGQELAHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKEVQFLRPEDPTQPSCFQPHRALRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPENFARPLEVAIEASNGASDYGNKFGEPVLAGFTRSFGLQLPDGQRREWIKPIMFSGGIGSMEAEHVRKEPPEPGMDIVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAARGNPICSLHDQGAGGNGNVLKELSDPAGAVIYTSRFQLGDPTLNALEIWGAEYQESNALLLRPLDRDFLSRVSARERCPTCFVGTITGDKRIVLVDDREYPVGRNGHEDASLSSPPTPVDLELDWVLGKMPRKEFFLKRSHPVLKPLALPPGLRVRQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLHTPLADVAVVALSHQELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGSETVRAPGSLVISAYAVCPDITATVTPDLKHPGGRGHLLYVSLSPGQHRLGGTALAQCFSQLGEQPPNLDLPENLVRAFSVTQGLLRDRLLCSGHDVSDGGLITCLLEMAFAGNCGIEVHVPAPGVDALSVLFAEEPGLVLEVQESDLAQVLKRYRVAGLHCMELGPTGDAGPHALVRVSVNGAVVLEEAVGQLRAVWEETSFQLDRLQAEPRCVAEEEQGLRERMGPRYCLPAAFPKASVPREPGGPVPRVAILREEGSNGDREMADAFHLAGFEVWDVTTQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPVARAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGSPSEEAKETKETSLDSWPAPPGLVLRHNLSGRFESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYMAFSSPELQAQIEARGLAPLHWADDNGKPTEQYPMNPNGSPGGVAGVCSLDGRHLAVMPHPERAVRPWQWAWRPPLFDTVTTSPWLQLFINARDWTRKGGC

Target ORF information:

RefSeq Version	XM_022418557.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418557.1

ORF Insert Sequence:

ATGACCTCTG TTCTTCACTT CTATGTCCAT CCCTCTGGCC ATGAGAGGGC AGCTTCAGAA 
CACACTCAAA GGAAGCTCCA AGGGAAGCTG CCAGCACTGC AGTCTGTCAA GACAGAGTTG 
TGCTACAATG TGAACTGGAC AGCCAAGTCC CCCCCAAACG CTGAAGAGAT GAAGAAGCTG 
ATGTGGCTTT TTGGTTGCCC CTTGTTACTG GGTGATGTTG CTCAGGAGTC CTGGCTCCAT 
CCCGACTCTA GTGACCTGCT GCTGGAGGTT GGGCCCAGGC TGAACTTCTC CACGCCAGCA 
TCCACCAACG TCGTGTCAGT GTGCTGGGCT GCTGGGCTGG GGGCTGTGGA TCGTGTGGAG 
ACTACCCGGC GCTACTTGCT CTCGTTTGCC CACCCCCCGT CAACGGAGAT GGAGACCATC 
GCTCTGGCTA CCCTGCATGA TCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGC 
TTCTCCCTCG GGAGCATTTC CACACCTCTC AACGGCCATA TCAACATCCT GGCTGAGGGC 
CGGTGTGCCC TGGAGAAAGC CAACCAGGAG CTAGGCCTGG CACTAGACTC CTGGGACCTA 
GATTTCTATA CCAAACGCTT CCAGGAGCTA CAGAGGAACC CAAGCACTGT GGAGGCTTTT 
GACTTGGCTC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
ATGGATGGTC AGGAGTTGGC ACACTCCCTG TTTGAGTCCA TCATGAGTAC CCAGGCATCC 
TCGAACCCCA ACAATGTGCT CAAGTTCTGT GACAACAGCA GCGCAATCCA GGGGAAGGAA 
GTCCAATTCC TAAGGCCCGA AGACCCCACT CAGCCAAGCT GCTTCCAGCC GCACCGGGCA 
CTGAGACATG TAGTCTTCAC AGCAGAGACC CACAACTTCC CCACAGGAGT AGCCCCCTTC 
AGTGGTGCGA CCACAGGCAC AGGGGGCCGG ATCCGAGATG TCCAGTGCAC GGGCCGAGGG 
GCCCACGTGG TGGCTGGCAC TGCTGGCTAT TGCTTTGGAA ACCTGCACAT CCCAGGTTAT 
AATCTGCCTT GGGAGGATCC AAGCTTCCAG TATCCTGAGA ACTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AGGCCAGTAA TGGGGCTTCT GATTACGGCA ACAAGTTTGG GGAGCCCGTG 
TTGGCCGGCT TCACCCGCTC CTTCGGACTC CAGCTCCCAG ACGGCCAGCG TCGTGAGTGG 
ATCAAGCCCA TCATGTTCAG TGGGGGCATC GGGTCCATGG AAGCCGAGCA TGTGCGCAAG 
GAGCCCCCAG AGCCAGGCAT GGACATTGTA AAGGTCGGGG GTCCTGTCTA TAGAATTGGA 
GTTGGCGGTG GAGCTGCGTC ATCTGTGCAA GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGGGG AGACCCGGAG ATGGAACAAA AGATGAACCG TGTGATCCGG 
GCATGTGTGG AGGCTGCCAG GGGCAACCCT ATCTGCAGCC TCCACGACCA GGGTGCTGGT 
GGCAACGGCA ACGTCCTGAA GGAACTGAGT GACCCCGCCG GAGCTGTCAT TTACACCAGC 
CGCTTCCAGC TCGGGGATCC AACCCTGAAC GCCCTGGAAA TCTGGGGGGC TGAGTATCAG 
GAGTCAAACG CACTTCTGCT GCGGCCCCTG GACCGGGATT TTCTCAGCCG TGTCAGCGCC 
CGGGAACGCT GCCCGACTTG CTTTGTGGGC ACCATTACAG GGGACAAGAG AATAGTGCTG 
GTGGATGATC GGGAGTACCC TGTTGGGAGA AATGGCCATG AGGATGCCTC CCTCTCATCT 
CCCCCAACCC CCGTGGACCT GGAGCTGGAT TGGGTGTTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTCA AGAGGAGTCA CCCCGTGCTG AAGCCTCTGG CCTTGCCCCC AGGTCTGCGA 
GTGCGCCAGG CCCTGGAGCG CGTCCTGAGG CTGCCTGCTG TGGCCAGCAA GCGCTACCTC 
ACTAACAAGG TGGACCGCTC TGTGGGTGGC CTGGTGGCAC AGCAGCAGTG CGTTGGACCC 
CTGCACACCC CTCTGGCAGA TGTGGCAGTT GTGGCGTTAA GTCACCAGGA GCTCATTGGA 
GCCGCCACAG CTCTGGGAGA GCAGCCAGTC AAAAGCCTGC TGGACCCAAA GGTTGCTGCC 
CGACTGGCTG TGGCTGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC CGACCTCCGG 
GACGTGAAGT GCAGTGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGAGA GGGTGCGGCC 
CTGGCCGACG CCTGTGAGGC TATGGTGGCA GTGATGGCGG CCTTGGGTGT GGCGGTGGAT 
GGTGGCAAGG ACTCTCTCAG CATGGCCGCC CGGGTCGGCT CTGAGACAGT GCGGGCACCT 
GGATCACTGG TCATTTCAGC TTATGCCGTC TGTCCAGACA TTACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGGAGG GAGAGGCCAT TTGCTGTACG TGTCTCTGAG CCCCGGGCAA 
CACCGGCTAG GGGGCACGGC CCTGGCCCAG TGTTTCTCGC AGCTAGGCGA GCAGCCTCCC 
AACCTGGACC TCCCTGAGAA CTTGGTGCGT GCCTTTAGCG TCACCCAGGG GCTGCTGAGA 
GACCGCCTCC TCTGCTCAGG CCATGATGTC AGTGACGGGG GTCTCATCAC CTGCCTGCTG 
GAGATGGCCT TTGCTGGGAA TTGTGGGATA GAGGTGCATG TGCCTGCCCC TGGGGTCGAT 
GCATTGTCTG TCCTGTTTGC TGAGGAGCCG GGCCTGGTGC TGGAGGTGCA GGAGTCCGAC 
CTGGCCCAGG TGCTGAAGCG CTATCGGGTT GCAGGCCTCC ACTGCATGGA GCTGGGCCCC 
ACGGGTGATG CCGGGCCCCA CGCCTTGGTG CGAGTATCGG TGAACGGGGC CGTGGTCCTG 
GAGGAGGCTG TTGGGCAGCT CCGAGCCGTC TGGGAGGAGA CCAGTTTCCA GCTGGACCGG 
CTGCAGGCAG AGCCCCGCTG CGTGGCCGAG GAAGAACAGG GGCTGAGGGA GCGGATGGGG 
CCCCGCTACT GCCTGCCCGC AGCCTTCCCC AAAGCTTCCG TGCCCCGTGA GCCTGGTGGG 
CCGGTGCCCC GAGTTGCCAT CTTGCGAGAG GAGGGCAGCA ACGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTGGCTGG CTTCGAGGTG TGGGACGTGA CCACGCAGGA CCTCTGCTCT 
GGGGCAATCG GTCTGGACAC GTTCCGTGGC GTGGCCTTCG TGGGCGGCTT TAGCTACGCA 
GATGTCCTGG GCTCTGCCAA GGGGTGGGCG GCCGCTGTGA CCTTCCACCC AGTGGCCAGG 
GCTGAACTGA GGCGCTTCCG GAAGCGGCCC GACACCTTTA GCCTGGGCGT GTGTAATGGC 
TGTCAGCTGC TGGCTCTGCT GGGCTGGGTA GGAGGCAGCC CCAGCGAGGA GGCCAAGGAG 
ACCAAGGAGA CCAGCCTGGA CTCCTGGCCA GCCCCGCCCG GCCTTGTGCT GCGCCACAAC 
CTGTCTGGAC GCTTTGAATC TCGCTGGGCC AGCGTGCGGG TGGGGCCCGG GCCGGCCCTG 
ATGCTGCGCG GGATGGAGGG TGCCGTGCTG CCCGTGTGGA GCGCTCACGG GGAAGGTTAC 
ATGGCGTTTT CTTCTCCGGA GCTCCAAGCC CAGATTGAGG CCAGGGGCTT GGCTCCGCTG 
CACTGGGCAG ACGACAATGG GAAGCCCACA GAACAGTACC CCATGAACCC CAACGGGTCT 
CCAGGAGGGG TGGCCGGCGT CTGCTCCCTT GATGGCCGCC ACCTGGCTGT CATGCCTCAC 
CCTGAGCGGG CTGTGAGGCC CTGGCAGTGG GCATGGCGCC CTCCTCTGTT TGACACTGTG 
ACCACCTCCC CCTGGCTCCA GCTCTTCATC AATGCTCGAG ACTGGACCCG GAAAGGAGGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PFAS cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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