NLRC5 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLRC5
Entrez Gene ID	487268
Full Name	NLR family CARD domain containing 5
General protein information	Preferred Names NLR family CARD domain containing 5 Names protein NLRC5
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_544394.5	XP_544394.5	protein NLRC5 isoform X1
XM_014109337.1	XP_013964812.1	protein NLRC5 isoform X2
XM_014109337.2	XP_013964812.1	protein NLRC5 isoform X2
XM_014109333.1	XP_013964808.1	protein NLRC5 isoform X1
XM_544394.6	XP_544394.5	protein NLRC5 isoform X1
XM_014109333.2	XP_013964808.1	protein NLRC5 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NLRC5	NP_115582.4
Gallus gallus (chicken)	NLRC5	XP_004944113.1
Bos taurus (cattle)	NLRC5	XP_002694836.1
Danio rerio (zebrafish)	si:ch211-122c9.5	XP_003200494.2
Xenopus tropicalis (tropical clawed frog)	LOC101730474	XP_004914291.1
Pan troglodytes (chimpanzee)	NLRC5	XP_003952898.1
	NLRC5	XP_001095341.2
Mus musculus (house mouse)	Nlrc5	NP_001028379.2
Canis lupus familiaris (dog)	NLRC5	XP_544394.4

Gene Ontology

The following NLRC5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRC5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe35403	XM_014109333.2 Latest version!	Canis lupus familiaris NLR family CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe35403	XM_544394.6 Latest version!	Canis lupus familiaris NLR family CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe35404	XM_014109337.2 Latest version!	Canis lupus familiaris NLR family CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
OCe35404	XM_014109337.1	Canis lupus familiaris NLR family, CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe35403	XM_544394.5	Canis lupus familiaris NLR family, CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe35403	XM_014109333.1	Canis lupus familiaris NLR family, CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLRC5 ( XM_544394.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_544394.5 Canis lupus familiaris NLR family, CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

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CloneID	OCe35403
Clone ID Related Accession (Same CDS sequence)	XM_544394.5 , XM_014109333.1 , XM_544394.6 , XM_014109333.2
Accession Version	XM_544394.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein NLRC5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_544394.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGCTTT 
AAGAGCAGGA AATGTGGGGA TGCCTTTGCA GAAGCCCTCT CCAAGAGCCT GCCAACAATG 
GAGAGCCTGA AGAAGCTCGG GTTAGCAGGA TGTAAGATCA CTGCCCGAGG CATCAGCCAC 
CTGATGCAGG CTTTGCGCCT CTGTCCACAG CTGGAAGAGA TCAGCTTCCA GGACAACCAG 
CTGAAGGATA GGGCCGTGCT GAATATCGTG GAAGTACTTC CTTCTTTGCC ACGGCTCCAG 
AAGCTTGACC TGAGCCGCAG CAATGTCTCC GTGTCAACCT TACTCTGCTT GACAAAGGTG 
GCGGTCACAT GCCCTACTGT CAAGATGCTA CAGGTCAGGG AGACGGACCT CATCTTTCTT 
CTTTCCCCGC CCACAGAGAC TGCTGCAGAG CTTGGAGCAG CACCAGATCT ACAGGGAAAT 
GCCATCCAGA GGAAAGAGGC TCAGAGCAGA AGCCTGGCAC TCAGGCTCCT GCAGTGCCAG 
CTCGGAATTC ATGATGTGCA GGAGCTCATT GCCCAGCTCC GGGAAGGTCC CCGCCTGGAG 
GAAGTAAACC TTTCAGGAAA CCAGCTGGAT GATGAAGGCT GTCGACTGAT GGCAGAAGCT 
GCATCCCAGC TGCACATCAC CAGGAATCTA GACCTCAGCA ACAACGGGCT ATCTGTGGAC 
AGGGTACACT GTATGCTGAG TGCGGTGAAC ACATGCCAGA ACTTGGCAGA GCTTCATATC 
AGCCTGCTGC ATAAAACTGT GGTCTTCACA TTTGCCCCAG AGCAGGAGGA GCAGGAGGGG 
ATCTGGAAGA GGACTGCGCT TCGGGACAGC CTCACGCACC AGGTGCCCTT TGAGCTGCCC 
CTGCGCTCCA CGAGGATCAG GCTGACACAC TGTGGCTTGC AAGCCAAGCA CCTAGAGCAG 
CTCTGCAGGG CCCTGGGAGG AAGCTGCCAC CACAGTCACC TGGACCTCTC AGGCAATGCT 
CTGGGGGATG AAGGCGTGGC CCAGCTGGCT CAGCTGCTCC CAGGGCTGGG TGCTCTGCAG 
TCCTTGAACC TCAGTGAGAA TGGTTTGTCC CTGGATACAG TGTTTATTTT GATCCAGTGC 
TTCTCGACTC TGCAGTGGCT TCTTCACTTG GATATCGGCT TCGAGAGCCA GCACGTCGTC 
CTGAGAGGTG ACAGAAGAGG CAGGGATCGA TTGGCTGGTG GATGTCCGCC AGAGTTCCTA 
GCTGGAGTCC CATTCTTGGC CTTTGATCAG TGCTGCGTCC CCGAGAGCTT CTGCCTCAGG 
CAATGTCAGC TGGAGCCCCT GAACCTCACC CGCCTCTGCG AGACTCTGGA GAAGTGCCCC 
GGGCCTCTGG AAGTCGAATT GTCCTTTGAG GTCCTGAGCG ACCAGAGCCT GGAGACCCTG 
CTGCAGCACT TACCCCGGCT GCCCCAGCTA AGCCTGCTGC AGCTAAGCCA GACGAGGCTT 
TCCCCAAAGA GCCTCCTCCT GCTGGCCGAC CTCTTCAGCT TATGCCCCCG GGTTCAGAAG 
GTGGATATCA GGTCCCTGTA TCACATGACA CTGCACTTCA GGTCTGGCGA GGAGCATGAA 
GGCGAGTGCT GTGGCAGGTT CACGGGCTGT GGCCTTAGTC AGGAGCATGT GGAGCCGCTC 
TGCTGGTTGC TGAGCAAATG CGAAGACCTC AACCAGTTGG ACCTCTCAGC AAACATGCTG 
GGTGATGACG GGCTCAGGTG CCTCCTGGAA TGTCTGCCTC AATTGCCCAT CTCTGGTTCG 
CTTGACCTGA GTCACAACAG CATCTCCCTA GAGAGTGCCC TGGGCCTGGT GAAGACACTC 
CCCTCCTGCC CGCACATCCG GGAGGCTTCT GTGAACCTGG GCTCTGAGCA GAGCTTCTGG 
ATCTACTTCT CCCGAAAGGA GGAGGCCGGG AAGACACTAA GGCTGAGCCA GTGCAACCTC 
ATGCCAGAGC ATGTGCCCAG ACTGGCCACC CACCTGAACC AGGCCCTGAA GCTGACAGCG 
CTCACGCTGA CCCAGTGCGG CCTGGGCCTG GAGCAGCTGA TAGTTCTCCT GAGTCTGCTG 
AGATGTCCAG TGGACCTGTT CACTCTCAGG GTGGAGGAGC CGTGGGTAGG CAAAGCCGGG 
GTGCTCGCCC TGCTAAGAGT CTGCACCCAG ACCTCAGGCA ACATCACTGA AATAAGCATT 
TCGGAGACCC AGCAGCAGCT CTGTCTCCAG CTGGAGTTTC CCCCTCAGGA GAACCCAGAA 
GCCGTGACGC TCAGGTTGGC TCACTGTGAC CTTGAGACCC ACCACAGCCT TCTTGCTGGG 
CAGCTGATGG AGACATGTGC CAGGCTGCAG CAGCTCAGCT TGTCCCAGGT GAACCTCTGC 
GACTCCAGCT CCCTGCTGCT AAAAAACCTC CTGGTGTCTC TCTCTGAGCT GAAGACATTC 
AGACTGACCT CCAGCTGTAT GAGCTCCAAG GGCCTGGCCC ACCTGACATT TGGCCTGAGA 
AACTGCCACC ACCTGGAGGA GCTGGACTTG TCCAACAATC AGTTTGGTGA GGAGGACACT 
GAAGTCCTGA TGACGGTCCT TGAGGGCAAG TGCTGGCTGA AGAAGCTCGA CCTCAGCCAT 
CTCCCACTAG GCAGCTCCAC CCTGGCCATG TTCACTCAAG GACTAAGCCA CATGACCCTC 
CTACAGAGCC TCCGGCTGAG CAGGAACGGT ATTTGTGATG TCGGCTGCCA CCAACTTTCC 
GAGGCCCTCA GAGCTGCCAT GAGCTTGAAG GAGCTGGGCT TGAGCCACAA CCAGATCAGA 
GACACCGGTG CCCAGCACTT AGCTGCTGTC CTACCAGGAC TGCCGGAGCT CAGGAAGATA 
GACCTCTCAG GGAATGGCAT TGGTCCAGCT GGAGGGACGC GGCTGGCAGA GTCCCTCACC 
CTCTGCAGGC ACTTGGAGGA GCTGATGCTT GGCTACAACG TCCTGGGGGA TACCACAGCC 
CTGGGCCTGG CCCAGGGACT GCCCTGCTGC CTGAGGGTCC TGCACCTGCC GTCTAGCCGT 
CTGGGCCCAA AGGGAGCGCT GAGCCTGAGC CAGGCCCTGG ATGGATGCCC ACATGTGGAA 
GAGATCAGCT TGGCAGAGAA CAGTCTGGCC AGAGGGGTCC TGCATTTCTG TCAGGGGCTC 
CCCCTGCTGA GGCAGATAGA CCTGGTTTCG TGTGAGATCG ACAACCACAC CGCCAAGCCC 
CTGGCTGCCA GCCTGGTGCT CTGCCCAGCC CTGGAAGAAA TCCTGCTGTC CTGGAATCTG 
CTCGGGGACG AGGCGGCTGC CGAGTTGGCC CGGGTCCTGC CGCAGATGGG CCGGCTGAAG 
AGAATGGACC TGGAGAAGAA TCGGATCACA GCTTGTGGAG CCTGGCTCCT GGCTGAAGGG 
CTGGCACAAG GGTCTGGCAT CCAAGTCATT CGCCTCTGGA ACAATCCCAT CTCCCCCACC 
ATGGTCCAGC ATCTACAGAG CCAGGAGCCC AGGCTGGACT TCGCTTTCTT CGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_544394.5
CDS	291..5870
Translation	MDHVSFQLGTTNLWSWLVRLLSKDPEWLNAKVKFFLPKMDLSSGDEAQDTTQRVMLQLRRLHTQGRATWQAFIHCVCMELDVPLDLEVPLLSTWGHGDGFPSQLGAGEESQPASQLHHGLKRPHQSCGSSPRLKQCKNHQLELARRYLKLLRTSAQQRYSRIPGPGQPLAFHQAYIPPILQWSRTTAPFSAQDGAVVGDPKAEDDTDVSIRDLFSTRADKGPRVTVLLGKAGMGKTTLAHRLCQRWAAGQLDRFQALFLFEFRQLNLITSSVTLPQLLFDLYLSPEAGPDSVFQYLEEHAGGVLLIFDGLDQALHSHSSTEPEGPEAPSPALALFSGLCRGTILPGCWVLATSRPGKLPDCLPTEAATVHMWGFDRPRVEEYVGRFFSDQEWQEAALAELRENRHLQSMCAVPALCQVTCLCLHHLLPGNSPGQSAALLPTVTQNYVQMVSTLSPRGYLPPESLLGLSEMALRGLETGKVIFSVGDISPPMVAFGAALGLLTSFCICTGPGHQGIGYAFTHLSLQEFFAALYLMASPKVDKNVLARHVTLNSRWVLRTKARLGLLDHLPTFLAGLASCACRPFLSHVAQQDEMWVSDKQAAVMLALRKLAIRRLTGPKVVALCHCVGETQEPELASLMAQSLPSHLSFHNFPLTYTDLSALTNILRHRNASIHLDFEGCPLEPHCPEALAGCKQIENLSFKSRKCGDAFAEALSKSLPTMESLKKLGLAGCKITARGISHLMQALRLCPQLEEISFQDNQLKDRAVLNIVEVLPSLPRLQKLDLSRSNVSVSTLLCLTKVAVTCPTVKMLQVRETDLIFLLSPPTETAAELGAAPDLQGNAIQRKEAQSRSLALRLLQCQLGIHDVQELIAQLREGPRLEEVNLSGNQLDDEGCRLMAEAASQLHITRNLDLSNNGLSVDRVHCMLSAVNTCQNLAELHISLLHKTVVFTFAPEQEEQEGIWKRTALRDSLTHQVPFELPLRSTRIRLTHCGLQAKHLEQLCRALGGSCHHSHLDLSGNALGDEGVAQLAQLLPGLGALQSLNLSENGLSLDTVFILIQCFSTLQWLLHLDIGFESQHVVLRGDRRGRDRLAGGCPPEFLAGVPFLAFDQCCVPESFCLRQCQLEPLNLTRLCETLEKCPGPLEVELSFEVLSDQSLETLLQHLPRLPQLSLLQLSQTRLSPKSLLLLADLFSLCPRVQKVDIRSLYHMTLHFRSGEEHEGECCGRFTGCGLSQEHVEPLCWLLSKCEDLNQLDLSANMLGDDGLRCLLECLPQLPISGSLDLSHNSISLESALGLVKTLPSCPHIREASVNLGSEQSFWIYFSRKEEAGKTLRLSQCNLMPEHVPRLATHLNQALKLTALTLTQCGLGLEQLIVLLSLLRCPVDLFTLRVEEPWVGKAGVLALLRVCTQTSGNITEISISETQQQLCLQLEFPPQENPEAVTLRLAHCDLETHHSLLAGQLMETCARLQQLSLSQVNLCDSSSLLLKNLLVSLSELKTFRLTSSCMSSKGLAHLTFGLRNCHHLEELDLSNNQFGEEDTEVLMTVLEGKCWLKKLDLSHLPLGSSTLAMFTQGLSHMTLLQSLRLSRNGICDVGCHQLSEALRAAMSLKELGLSHNQIRDTGAQHLAAVLPGLPELRKIDLSGNGIGPAGGTRLAESLTLCRHLEELMLGYNVLGDTTALGLAQGLPCCLRVLHLPSSRLGPKGALSLSQALDGCPHVEEISLAENSLARGVLHFCQGLPLLRQIDLVSCEIDNHTAKPLAASLVLCPALEEILLSWNLLGDEAAAELARVLPQMGRLKRMDLEKNRITACGAWLLAEGLAQGSGIQVIRLWNNPISPTMVQHLQSQEPRLDFAFFDK

Target ORF information:

RefSeq Version	XM_544394.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NLR family, CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_544394.5

ORF Insert Sequence:

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGCTTT 
AAGAGCAGGA AATGTGGGGA TGCCTTTGCA GAAGCCCTCT CCAAGAGCCT GCCAACAATG 
GAGAGCCTGA AGAAGCTCGG GTTAGCAGGA TGTAAGATCA CTGCCCGAGG CATCAGCCAC 
CTGATGCAGG CTTTGCGCCT CTGTCCACAG CTGGAAGAGA TCAGCTTCCA GGACAACCAG 
CTGAAGGATA GGGCCGTGCT GAATATCGTG GAAGTACTTC CTTCTTTGCC ACGGCTCCAG 
AAGCTTGACC TGAGCCGCAG CAATGTCTCC GTGTCAACCT TACTCTGCTT GACAAAGGTG 
GCGGTCACAT GCCCTACTGT CAAGATGCTA CAGGTCAGGG AGACGGACCT CATCTTTCTT 
CTTTCCCCGC CCACAGAGAC TGCTGCAGAG CTTGGAGCAG CACCAGATCT ACAGGGAAAT 
GCCATCCAGA GGAAAGAGGC TCAGAGCAGA AGCCTGGCAC TCAGGCTCCT GCAGTGCCAG 
CTCGGAATTC ATGATGTGCA GGAGCTCATT GCCCAGCTCC GGGAAGGTCC CCGCCTGGAG 
GAAGTAAACC TTTCAGGAAA CCAGCTGGAT GATGAAGGCT GTCGACTGAT GGCAGAAGCT 
GCATCCCAGC TGCACATCAC CAGGAATCTA GACCTCAGCA ACAACGGGCT ATCTGTGGAC 
AGGGTACACT GTATGCTGAG TGCGGTGAAC ACATGCCAGA ACTTGGCAGA GCTTCATATC 
AGCCTGCTGC ATAAAACTGT GGTCTTCACA TTTGCCCCAG AGCAGGAGGA GCAGGAGGGG 
ATCTGGAAGA GGACTGCGCT TCGGGACAGC CTCACGCACC AGGTGCCCTT TGAGCTGCCC 
CTGCGCTCCA CGAGGATCAG GCTGACACAC TGTGGCTTGC AAGCCAAGCA CCTAGAGCAG 
CTCTGCAGGG CCCTGGGAGG AAGCTGCCAC CACAGTCACC TGGACCTCTC AGGCAATGCT 
CTGGGGGATG AAGGCGTGGC CCAGCTGGCT CAGCTGCTCC CAGGGCTGGG TGCTCTGCAG 
TCCTTGAACC TCAGTGAGAA TGGTTTGTCC CTGGATACAG TGTTTATTTT GATCCAGTGC 
TTCTCGACTC TGCAGTGGCT TCTTCACTTG GATATCGGCT TCGAGAGCCA GCACGTCGTC 
CTGAGAGGTG ACAGAAGAGG CAGGGATCGA TTGGCTGGTG GATGTCCGCC AGAGTTCCTA 
GCTGGAGTCC CATTCTTGGC CTTTGATCAG TGCTGCGTCC CCGAGAGCTT CTGCCTCAGG 
CAATGTCAGC TGGAGCCCCT GAACCTCACC CGCCTCTGCG AGACTCTGGA GAAGTGCCCC 
GGGCCTCTGG AAGTCGAATT GTCCTTTGAG GTCCTGAGCG ACCAGAGCCT GGAGACCCTG 
CTGCAGCACT TACCCCGGCT GCCCCAGCTA AGCCTGCTGC AGCTAAGCCA GACGAGGCTT 
TCCCCAAAGA GCCTCCTCCT GCTGGCCGAC CTCTTCAGCT TATGCCCCCG GGTTCAGAAG 
GTGGATATCA GGTCCCTGTA TCACATGACA CTGCACTTCA GGTCTGGCGA GGAGCATGAA 
GGCGAGTGCT GTGGCAGGTT CACGGGCTGT GGCCTTAGTC AGGAGCATGT GGAGCCGCTC 
TGCTGGTTGC TGAGCAAATG CGAAGACCTC AACCAGTTGG ACCTCTCAGC AAACATGCTG 
GGTGATGACG GGCTCAGGTG CCTCCTGGAA TGTCTGCCTC AATTGCCCAT CTCTGGTTCG 
CTTGACCTGA GTCACAACAG CATCTCCCTA GAGAGTGCCC TGGGCCTGGT GAAGACACTC 
CCCTCCTGCC CGCACATCCG GGAGGCTTCT GTGAACCTGG GCTCTGAGCA GAGCTTCTGG 
ATCTACTTCT CCCGAAAGGA GGAGGCCGGG AAGACACTAA GGCTGAGCCA GTGCAACCTC 
ATGCCAGAGC ATGTGCCCAG ACTGGCCACC CACCTGAACC AGGCCCTGAA GCTGACAGCG 
CTCACGCTGA CCCAGTGCGG CCTGGGCCTG GAGCAGCTGA TAGTTCTCCT GAGTCTGCTG 
AGATGTCCAG TGGACCTGTT CACTCTCAGG GTGGAGGAGC CGTGGGTAGG CAAAGCCGGG 
GTGCTCGCCC TGCTAAGAGT CTGCACCCAG ACCTCAGGCA ACATCACTGA AATAAGCATT 
TCGGAGACCC AGCAGCAGCT CTGTCTCCAG CTGGAGTTTC CCCCTCAGGA GAACCCAGAA 
GCCGTGACGC TCAGGTTGGC TCACTGTGAC CTTGAGACCC ACCACAGCCT TCTTGCTGGG 
CAGCTGATGG AGACATGTGC CAGGCTGCAG CAGCTCAGCT TGTCCCAGGT GAACCTCTGC 
GACTCCAGCT CCCTGCTGCT AAAAAACCTC CTGGTGTCTC TCTCTGAGCT GAAGACATTC 
AGACTGACCT CCAGCTGTAT GAGCTCCAAG GGCCTGGCCC ACCTGACATT TGGCCTGAGA 
AACTGCCACC ACCTGGAGGA GCTGGACTTG TCCAACAATC AGTTTGGTGA GGAGGACACT 
GAAGTCCTGA TGACGGTCCT TGAGGGCAAG TGCTGGCTGA AGAAGCTCGA CCTCAGCCAT 
CTCCCACTAG GCAGCTCCAC CCTGGCCATG TTCACTCAAG GACTAAGCCA CATGACCCTC 
CTACAGAGCC TCCGGCTGAG CAGGAACGGT ATTTGTGATG TCGGCTGCCA CCAACTTTCC 
GAGGCCCTCA GAGCTGCCAT GAGCTTGAAG GAGCTGGGCT TGAGCCACAA CCAGATCAGA 
GACACCGGTG CCCAGCACTT AGCTGCTGTC CTACCAGGAC TGCCGGAGCT CAGGAAGATA 
GACCTCTCAG GGAATGGCAT TGGTCCAGCT GGAGGGACGC GGCTGGCAGA GTCCCTCACC 
CTCTGCAGGC ACTTGGAGGA GCTGATGCTT GGCTACAACG TCCTGGGGGA TACCACAGCC 
CTGGGCCTGG CCCAGGGACT GCCCTGCTGC CTGAGGGTCC TGCACCTGCC GTCTAGCCGT 
CTGGGCCCAA AGGGAGCGCT GAGCCTGAGC CAGGCCCTGG ATGGATGCCC ACATGTGGAA 
GAGATCAGCT TGGCAGAGAA CAGTCTGGCC AGAGGGGTCC TGCATTTCTG TCAGGGGCTC 
CCCCTGCTGA GGCAGATAGA CCTGGTTTCG TGTGAGATCG ACAACCACAC CGCCAAGCCC 
CTGGCTGCCA GCCTGGTGCT CTGCCCAGCC CTGGAAGAAA TCCTGCTGTC CTGGAATCTG 
CTCGGGGACG AGGCGGCTGC CGAGTTGGCC CGGGTCCTGC CGCAGATGGG CCGGCTGAAG 
AGAATGGACC TGGAGAAGAA TCGGATCACA GCTTGTGGAG CCTGGCTCCT GGCTGAAGGG 
CTGGCACAAG GGTCTGGCAT CCAAGTCATT CGCCTCTGGA ACAATCCCAT CTCCCCCACC 
ATGGTCCAGC ATCTACAGAG CCAGGAGCCC AGGCTGGACT TCGCTTTCTT CGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_014109333.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964808.1 Canis lupus familiaris NLR family, CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.

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CloneID	OCe35403
Clone ID Related Accession (Same CDS sequence)	XM_544394.5 , XM_014109333.1 , XM_544394.6 , XM_014109333.2
Accession Version	XM_014109333.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein NLRC5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGCTTT 
AAGAGCAGGA AATGTGGGGA TGCCTTTGCA GAAGCCCTCT CCAAGAGCCT GCCAACAATG 
GAGAGCCTGA AGAAGCTCGG GTTAGCAGGA TGTAAGATCA CTGCCCGAGG CATCAGCCAC 
CTGATGCAGG CTTTGCGCCT CTGTCCACAG CTGGAAGAGA TCAGCTTCCA GGACAACCAG 
CTGAAGGATA GGGCCGTGCT GAATATCGTG GAAGTACTTC CTTCTTTGCC ACGGCTCCAG 
AAGCTTGACC TGAGCCGCAG CAATGTCTCC GTGTCAACCT TACTCTGCTT GACAAAGGTG 
GCGGTCACAT GCCCTACTGT CAAGATGCTA CAGGTCAGGG AGACGGACCT CATCTTTCTT 
CTTTCCCCGC CCACAGAGAC TGCTGCAGAG CTTGGAGCAG CACCAGATCT ACAGGGAAAT 
GCCATCCAGA GGAAAGAGGC TCAGAGCAGA AGCCTGGCAC TCAGGCTCCT GCAGTGCCAG 
CTCGGAATTC ATGATGTGCA GGAGCTCATT GCCCAGCTCC GGGAAGGTCC CCGCCTGGAG 
GAAGTAAACC TTTCAGGAAA CCAGCTGGAT GATGAAGGCT GTCGACTGAT GGCAGAAGCT 
GCATCCCAGC TGCACATCAC CAGGAATCTA GACCTCAGCA ACAACGGGCT ATCTGTGGAC 
AGGGTACACT GTATGCTGAG TGCGGTGAAC ACATGCCAGA ACTTGGCAGA GCTTCATATC 
AGCCTGCTGC ATAAAACTGT GGTCTTCACA TTTGCCCCAG AGCAGGAGGA GCAGGAGGGG 
ATCTGGAAGA GGACTGCGCT TCGGGACAGC CTCACGCACC AGGTGCCCTT TGAGCTGCCC 
CTGCGCTCCA CGAGGATCAG GCTGACACAC TGTGGCTTGC AAGCCAAGCA CCTAGAGCAG 
CTCTGCAGGG CCCTGGGAGG AAGCTGCCAC CACAGTCACC TGGACCTCTC AGGCAATGCT 
CTGGGGGATG AAGGCGTGGC CCAGCTGGCT CAGCTGCTCC CAGGGCTGGG TGCTCTGCAG 
TCCTTGAACC TCAGTGAGAA TGGTTTGTCC CTGGATACAG TGTTTATTTT GATCCAGTGC 
TTCTCGACTC TGCAGTGGCT TCTTCACTTG GATATCGGCT TCGAGAGCCA GCACGTCGTC 
CTGAGAGGTG ACAGAAGAGG CAGGGATCGA TTGGCTGGTG GATGTCCGCC AGAGTTCCTA 
GCTGGAGTCC CATTCTTGGC CTTTGATCAG TGCTGCGTCC CCGAGAGCTT CTGCCTCAGG 
CAATGTCAGC TGGAGCCCCT GAACCTCACC CGCCTCTGCG AGACTCTGGA GAAGTGCCCC 
GGGCCTCTGG AAGTCGAATT GTCCTTTGAG GTCCTGAGCG ACCAGAGCCT GGAGACCCTG 
CTGCAGCACT TACCCCGGCT GCCCCAGCTA AGCCTGCTGC AGCTAAGCCA GACGAGGCTT 
TCCCCAAAGA GCCTCCTCCT GCTGGCCGAC CTCTTCAGCT TATGCCCCCG GGTTCAGAAG 
GTGGATATCA GGTCCCTGTA TCACATGACA CTGCACTTCA GGTCTGGCGA GGAGCATGAA 
GGCGAGTGCT GTGGCAGGTT CACGGGCTGT GGCCTTAGTC AGGAGCATGT GGAGCCGCTC 
TGCTGGTTGC TGAGCAAATG CGAAGACCTC AACCAGTTGG ACCTCTCAGC AAACATGCTG 
GGTGATGACG GGCTCAGGTG CCTCCTGGAA TGTCTGCCTC AATTGCCCAT CTCTGGTTCG 
CTTGACCTGA GTCACAACAG CATCTCCCTA GAGAGTGCCC TGGGCCTGGT GAAGACACTC 
CCCTCCTGCC CGCACATCCG GGAGGCTTCT GTGAACCTGG GCTCTGAGCA GAGCTTCTGG 
ATCTACTTCT CCCGAAAGGA GGAGGCCGGG AAGACACTAA GGCTGAGCCA GTGCAACCTC 
ATGCCAGAGC ATGTGCCCAG ACTGGCCACC CACCTGAACC AGGCCCTGAA GCTGACAGCG 
CTCACGCTGA CCCAGTGCGG CCTGGGCCTG GAGCAGCTGA TAGTTCTCCT GAGTCTGCTG 
AGATGTCCAG TGGACCTGTT CACTCTCAGG GTGGAGGAGC CGTGGGTAGG CAAAGCCGGG 
GTGCTCGCCC TGCTAAGAGT CTGCACCCAG ACCTCAGGCA ACATCACTGA AATAAGCATT 
TCGGAGACCC AGCAGCAGCT CTGTCTCCAG CTGGAGTTTC CCCCTCAGGA GAACCCAGAA 
GCCGTGACGC TCAGGTTGGC TCACTGTGAC CTTGAGACCC ACCACAGCCT TCTTGCTGGG 
CAGCTGATGG AGACATGTGC CAGGCTGCAG CAGCTCAGCT TGTCCCAGGT GAACCTCTGC 
GACTCCAGCT CCCTGCTGCT AAAAAACCTC CTGGTGTCTC TCTCTGAGCT GAAGACATTC 
AGACTGACCT CCAGCTGTAT GAGCTCCAAG GGCCTGGCCC ACCTGACATT TGGCCTGAGA 
AACTGCCACC ACCTGGAGGA GCTGGACTTG TCCAACAATC AGTTTGGTGA GGAGGACACT 
GAAGTCCTGA TGACGGTCCT TGAGGGCAAG TGCTGGCTGA AGAAGCTCGA CCTCAGCCAT 
CTCCCACTAG GCAGCTCCAC CCTGGCCATG TTCACTCAAG GACTAAGCCA CATGACCCTC 
CTACAGAGCC TCCGGCTGAG CAGGAACGGT ATTTGTGATG TCGGCTGCCA CCAACTTTCC 
GAGGCCCTCA GAGCTGCCAT GAGCTTGAAG GAGCTGGGCT TGAGCCACAA CCAGATCAGA 
GACACCGGTG CCCAGCACTT AGCTGCTGTC CTACCAGGAC TGCCGGAGCT CAGGAAGATA 
GACCTCTCAG GGAATGGCAT TGGTCCAGCT GGAGGGACGC GGCTGGCAGA GTCCCTCACC 
CTCTGCAGGC ACTTGGAGGA GCTGATGCTT GGCTACAACG TCCTGGGGGA TACCACAGCC 
CTGGGCCTGG CCCAGGGACT GCCCTGCTGC CTGAGGGTCC TGCACCTGCC GTCTAGCCGT 
CTGGGCCCAA AGGGAGCGCT GAGCCTGAGC CAGGCCCTGG ATGGATGCCC ACATGTGGAA 
GAGATCAGCT TGGCAGAGAA CAGTCTGGCC AGAGGGGTCC TGCATTTCTG TCAGGGGCTC 
CCCCTGCTGA GGCAGATAGA CCTGGTTTCG TGTGAGATCG ACAACCACAC CGCCAAGCCC 
CTGGCTGCCA GCCTGGTGCT CTGCCCAGCC CTGGAAGAAA TCCTGCTGTC CTGGAATCTG 
CTCGGGGACG AGGCGGCTGC CGAGTTGGCC CGGGTCCTGC CGCAGATGGG CCGGCTGAAG 
AGAATGGACC TGGAGAAGAA TCGGATCACA GCTTGTGGAG CCTGGCTCCT GGCTGAAGGG 
CTGGCACAAG GGTCTGGCAT CCAAGTCATT CGCCTCTGGA ACAATCCCAT CTCCCCCACC 
ATGGTCCAGC ATCTACAGAG CCAGGAGCCC AGGCTGGACT TCGCTTTCTT CGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964808.1
CDS	217..5796
Translation	MDHVSFQLGTTNLWSWLVRLLSKDPEWLNAKVKFFLPKMDLSSGDEAQDTTQRVMLQLRRLHTQGRATWQAFIHCVCMELDVPLDLEVPLLSTWGHGDGFPSQLGAGEESQPASQLHHGLKRPHQSCGSSPRLKQCKNHQLELARRYLKLLRTSAQQRYSRIPGPGQPLAFHQAYIPPILQWSRTTAPFSAQDGAVVGDPKAEDDTDVSIRDLFSTRADKGPRVTVLLGKAGMGKTTLAHRLCQRWAAGQLDRFQALFLFEFRQLNLITSSVTLPQLLFDLYLSPEAGPDSVFQYLEEHAGGVLLIFDGLDQALHSHSSTEPEGPEAPSPALALFSGLCRGTILPGCWVLATSRPGKLPDCLPTEAATVHMWGFDRPRVEEYVGRFFSDQEWQEAALAELRENRHLQSMCAVPALCQVTCLCLHHLLPGNSPGQSAALLPTVTQNYVQMVSTLSPRGYLPPESLLGLSEMALRGLETGKVIFSVGDISPPMVAFGAALGLLTSFCICTGPGHQGIGYAFTHLSLQEFFAALYLMASPKVDKNVLARHVTLNSRWVLRTKARLGLLDHLPTFLAGLASCACRPFLSHVAQQDEMWVSDKQAAVMLALRKLAIRRLTGPKVVALCHCVGETQEPELASLMAQSLPSHLSFHNFPLTYTDLSALTNILRHRNASIHLDFEGCPLEPHCPEALAGCKQIENLSFKSRKCGDAFAEALSKSLPTMESLKKLGLAGCKITARGISHLMQALRLCPQLEEISFQDNQLKDRAVLNIVEVLPSLPRLQKLDLSRSNVSVSTLLCLTKVAVTCPTVKMLQVRETDLIFLLSPPTETAAELGAAPDLQGNAIQRKEAQSRSLALRLLQCQLGIHDVQELIAQLREGPRLEEVNLSGNQLDDEGCRLMAEAASQLHITRNLDLSNNGLSVDRVHCMLSAVNTCQNLAELHISLLHKTVVFTFAPEQEEQEGIWKRTALRDSLTHQVPFELPLRSTRIRLTHCGLQAKHLEQLCRALGGSCHHSHLDLSGNALGDEGVAQLAQLLPGLGALQSLNLSENGLSLDTVFILIQCFSTLQWLLHLDIGFESQHVVLRGDRRGRDRLAGGCPPEFLAGVPFLAFDQCCVPESFCLRQCQLEPLNLTRLCETLEKCPGPLEVELSFEVLSDQSLETLLQHLPRLPQLSLLQLSQTRLSPKSLLLLADLFSLCPRVQKVDIRSLYHMTLHFRSGEEHEGECCGRFTGCGLSQEHVEPLCWLLSKCEDLNQLDLSANMLGDDGLRCLLECLPQLPISGSLDLSHNSISLESALGLVKTLPSCPHIREASVNLGSEQSFWIYFSRKEEAGKTLRLSQCNLMPEHVPRLATHLNQALKLTALTLTQCGLGLEQLIVLLSLLRCPVDLFTLRVEEPWVGKAGVLALLRVCTQTSGNITEISISETQQQLCLQLEFPPQENPEAVTLRLAHCDLETHHSLLAGQLMETCARLQQLSLSQVNLCDSSSLLLKNLLVSLSELKTFRLTSSCMSSKGLAHLTFGLRNCHHLEELDLSNNQFGEEDTEVLMTVLEGKCWLKKLDLSHLPLGSSTLAMFTQGLSHMTLLQSLRLSRNGICDVGCHQLSEALRAAMSLKELGLSHNQIRDTGAQHLAAVLPGLPELRKIDLSGNGIGPAGGTRLAESLTLCRHLEELMLGYNVLGDTTALGLAQGLPCCLRVLHLPSSRLGPKGALSLSQALDGCPHVEEISLAENSLARGVLHFCQGLPLLRQIDLVSCEIDNHTAKPLAASLVLCPALEEILLSWNLLGDEAAAELARVLPQMGRLKRMDLEKNRITACGAWLLAEGLAQGSGIQVIRLWNNPISPTMVQHLQSQEPRLDFAFFDK

Target ORF information:

RefSeq Version	XM_014109333.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NLR family, CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109333.1

ORF Insert Sequence:

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGCTTT 
AAGAGCAGGA AATGTGGGGA TGCCTTTGCA GAAGCCCTCT CCAAGAGCCT GCCAACAATG 
GAGAGCCTGA AGAAGCTCGG GTTAGCAGGA TGTAAGATCA CTGCCCGAGG CATCAGCCAC 
CTGATGCAGG CTTTGCGCCT CTGTCCACAG CTGGAAGAGA TCAGCTTCCA GGACAACCAG 
CTGAAGGATA GGGCCGTGCT GAATATCGTG GAAGTACTTC CTTCTTTGCC ACGGCTCCAG 
AAGCTTGACC TGAGCCGCAG CAATGTCTCC GTGTCAACCT TACTCTGCTT GACAAAGGTG 
GCGGTCACAT GCCCTACTGT CAAGATGCTA CAGGTCAGGG AGACGGACCT CATCTTTCTT 
CTTTCCCCGC CCACAGAGAC TGCTGCAGAG CTTGGAGCAG CACCAGATCT ACAGGGAAAT 
GCCATCCAGA GGAAAGAGGC TCAGAGCAGA AGCCTGGCAC TCAGGCTCCT GCAGTGCCAG 
CTCGGAATTC ATGATGTGCA GGAGCTCATT GCCCAGCTCC GGGAAGGTCC CCGCCTGGAG 
GAAGTAAACC TTTCAGGAAA CCAGCTGGAT GATGAAGGCT GTCGACTGAT GGCAGAAGCT 
GCATCCCAGC TGCACATCAC CAGGAATCTA GACCTCAGCA ACAACGGGCT ATCTGTGGAC 
AGGGTACACT GTATGCTGAG TGCGGTGAAC ACATGCCAGA ACTTGGCAGA GCTTCATATC 
AGCCTGCTGC ATAAAACTGT GGTCTTCACA TTTGCCCCAG AGCAGGAGGA GCAGGAGGGG 
ATCTGGAAGA GGACTGCGCT TCGGGACAGC CTCACGCACC AGGTGCCCTT TGAGCTGCCC 
CTGCGCTCCA CGAGGATCAG GCTGACACAC TGTGGCTTGC AAGCCAAGCA CCTAGAGCAG 
CTCTGCAGGG CCCTGGGAGG AAGCTGCCAC CACAGTCACC TGGACCTCTC AGGCAATGCT 
CTGGGGGATG AAGGCGTGGC CCAGCTGGCT CAGCTGCTCC CAGGGCTGGG TGCTCTGCAG 
TCCTTGAACC TCAGTGAGAA TGGTTTGTCC CTGGATACAG TGTTTATTTT GATCCAGTGC 
TTCTCGACTC TGCAGTGGCT TCTTCACTTG GATATCGGCT TCGAGAGCCA GCACGTCGTC 
CTGAGAGGTG ACAGAAGAGG CAGGGATCGA TTGGCTGGTG GATGTCCGCC AGAGTTCCTA 
GCTGGAGTCC CATTCTTGGC CTTTGATCAG TGCTGCGTCC CCGAGAGCTT CTGCCTCAGG 
CAATGTCAGC TGGAGCCCCT GAACCTCACC CGCCTCTGCG AGACTCTGGA GAAGTGCCCC 
GGGCCTCTGG AAGTCGAATT GTCCTTTGAG GTCCTGAGCG ACCAGAGCCT GGAGACCCTG 
CTGCAGCACT TACCCCGGCT GCCCCAGCTA AGCCTGCTGC AGCTAAGCCA GACGAGGCTT 
TCCCCAAAGA GCCTCCTCCT GCTGGCCGAC CTCTTCAGCT TATGCCCCCG GGTTCAGAAG 
GTGGATATCA GGTCCCTGTA TCACATGACA CTGCACTTCA GGTCTGGCGA GGAGCATGAA 
GGCGAGTGCT GTGGCAGGTT CACGGGCTGT GGCCTTAGTC AGGAGCATGT GGAGCCGCTC 
TGCTGGTTGC TGAGCAAATG CGAAGACCTC AACCAGTTGG ACCTCTCAGC AAACATGCTG 
GGTGATGACG GGCTCAGGTG CCTCCTGGAA TGTCTGCCTC AATTGCCCAT CTCTGGTTCG 
CTTGACCTGA GTCACAACAG CATCTCCCTA GAGAGTGCCC TGGGCCTGGT GAAGACACTC 
CCCTCCTGCC CGCACATCCG GGAGGCTTCT GTGAACCTGG GCTCTGAGCA GAGCTTCTGG 
ATCTACTTCT CCCGAAAGGA GGAGGCCGGG AAGACACTAA GGCTGAGCCA GTGCAACCTC 
ATGCCAGAGC ATGTGCCCAG ACTGGCCACC CACCTGAACC AGGCCCTGAA GCTGACAGCG 
CTCACGCTGA CCCAGTGCGG CCTGGGCCTG GAGCAGCTGA TAGTTCTCCT GAGTCTGCTG 
AGATGTCCAG TGGACCTGTT CACTCTCAGG GTGGAGGAGC CGTGGGTAGG CAAAGCCGGG 
GTGCTCGCCC TGCTAAGAGT CTGCACCCAG ACCTCAGGCA ACATCACTGA AATAAGCATT 
TCGGAGACCC AGCAGCAGCT CTGTCTCCAG CTGGAGTTTC CCCCTCAGGA GAACCCAGAA 
GCCGTGACGC TCAGGTTGGC TCACTGTGAC CTTGAGACCC ACCACAGCCT TCTTGCTGGG 
CAGCTGATGG AGACATGTGC CAGGCTGCAG CAGCTCAGCT TGTCCCAGGT GAACCTCTGC 
GACTCCAGCT CCCTGCTGCT AAAAAACCTC CTGGTGTCTC TCTCTGAGCT GAAGACATTC 
AGACTGACCT CCAGCTGTAT GAGCTCCAAG GGCCTGGCCC ACCTGACATT TGGCCTGAGA 
AACTGCCACC ACCTGGAGGA GCTGGACTTG TCCAACAATC AGTTTGGTGA GGAGGACACT 
GAAGTCCTGA TGACGGTCCT TGAGGGCAAG TGCTGGCTGA AGAAGCTCGA CCTCAGCCAT 
CTCCCACTAG GCAGCTCCAC CCTGGCCATG TTCACTCAAG GACTAAGCCA CATGACCCTC 
CTACAGAGCC TCCGGCTGAG CAGGAACGGT ATTTGTGATG TCGGCTGCCA CCAACTTTCC 
GAGGCCCTCA GAGCTGCCAT GAGCTTGAAG GAGCTGGGCT TGAGCCACAA CCAGATCAGA 
GACACCGGTG CCCAGCACTT AGCTGCTGTC CTACCAGGAC TGCCGGAGCT CAGGAAGATA 
GACCTCTCAG GGAATGGCAT TGGTCCAGCT GGAGGGACGC GGCTGGCAGA GTCCCTCACC 
CTCTGCAGGC ACTTGGAGGA GCTGATGCTT GGCTACAACG TCCTGGGGGA TACCACAGCC 
CTGGGCCTGG CCCAGGGACT GCCCTGCTGC CTGAGGGTCC TGCACCTGCC GTCTAGCCGT 
CTGGGCCCAA AGGGAGCGCT GAGCCTGAGC CAGGCCCTGG ATGGATGCCC ACATGTGGAA 
GAGATCAGCT TGGCAGAGAA CAGTCTGGCC AGAGGGGTCC TGCATTTCTG TCAGGGGCTC 
CCCCTGCTGA GGCAGATAGA CCTGGTTTCG TGTGAGATCG ACAACCACAC CGCCAAGCCC 
CTGGCTGCCA GCCTGGTGCT CTGCCCAGCC CTGGAAGAAA TCCTGCTGTC CTGGAATCTG 
CTCGGGGACG AGGCGGCTGC CGAGTTGGCC CGGGTCCTGC CGCAGATGGG CCGGCTGAAG 
AGAATGGACC TGGAGAAGAA TCGGATCACA GCTTGTGGAG CCTGGCTCCT GGCTGAAGGG 
CTGGCACAAG GGTCTGGCAT CCAAGTCATT CGCCTCTGGA ACAATCCCAT CTCCCCCACC 
ATGGTCCAGC ATCTACAGAG CCAGGAGCCC AGGCTGGACT TCGCTTTCTT CGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_544394.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_544394.5 Canis lupus familiaris NLR family CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

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CloneID	OCe35403
Clone ID Related Accession (Same CDS sequence)	XM_544394.5 , XM_014109333.1 , XM_544394.6 , XM_014109333.2
Accession Version	XM_544394.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein NLRC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_544394.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGCTTT 
AAGAGCAGGA AATGTGGGGA TGCCTTTGCA GAAGCCCTCT CCAAGAGCCT GCCAACAATG 
GAGAGCCTGA AGAAGCTCGG GTTAGCAGGA TGTAAGATCA CTGCCCGAGG CATCAGCCAC 
CTGATGCAGG CTTTGCGCCT CTGTCCACAG CTGGAAGAGA TCAGCTTCCA GGACAACCAG 
CTGAAGGATA GGGCCGTGCT GAATATCGTG GAAGTACTTC CTTCTTTGCC ACGGCTCCAG 
AAGCTTGACC TGAGCCGCAG CAATGTCTCC GTGTCAACCT TACTCTGCTT GACAAAGGTG 
GCGGTCACAT GCCCTACTGT CAAGATGCTA CAGGTCAGGG AGACGGACCT CATCTTTCTT 
CTTTCCCCGC CCACAGAGAC TGCTGCAGAG CTTGGAGCAG CACCAGATCT ACAGGGAAAT 
GCCATCCAGA GGAAAGAGGC TCAGAGCAGA AGCCTGGCAC TCAGGCTCCT GCAGTGCCAG 
CTCGGAATTC ATGATGTGCA GGAGCTCATT GCCCAGCTCC GGGAAGGTCC CCGCCTGGAG 
GAAGTAAACC TTTCAGGAAA CCAGCTGGAT GATGAAGGCT GTCGACTGAT GGCAGAAGCT 
GCATCCCAGC TGCACATCAC CAGGAATCTA GACCTCAGCA ACAACGGGCT ATCTGTGGAC 
AGGGTACACT GTATGCTGAG TGCGGTGAAC ACATGCCAGA ACTTGGCAGA GCTTCATATC 
AGCCTGCTGC ATAAAACTGT GGTCTTCACA TTTGCCCCAG AGCAGGAGGA GCAGGAGGGG 
ATCTGGAAGA GGACTGCGCT TCGGGACAGC CTCACGCACC AGGTGCCCTT TGAGCTGCCC 
CTGCGCTCCA CGAGGATCAG GCTGACACAC TGTGGCTTGC AAGCCAAGCA CCTAGAGCAG 
CTCTGCAGGG CCCTGGGAGG AAGCTGCCAC CACAGTCACC TGGACCTCTC AGGCAATGCT 
CTGGGGGATG AAGGCGTGGC CCAGCTGGCT CAGCTGCTCC CAGGGCTGGG TGCTCTGCAG 
TCCTTGAACC TCAGTGAGAA TGGTTTGTCC CTGGATACAG TGTTTATTTT GATCCAGTGC 
TTCTCGACTC TGCAGTGGCT TCTTCACTTG GATATCGGCT TCGAGAGCCA GCACGTCGTC 
CTGAGAGGTG ACAGAAGAGG CAGGGATCGA TTGGCTGGTG GATGTCCGCC AGAGTTCCTA 
GCTGGAGTCC CATTCTTGGC CTTTGATCAG TGCTGCGTCC CCGAGAGCTT CTGCCTCAGG 
CAATGTCAGC TGGAGCCCCT GAACCTCACC CGCCTCTGCG AGACTCTGGA GAAGTGCCCC 
GGGCCTCTGG AAGTCGAATT GTCCTTTGAG GTCCTGAGCG ACCAGAGCCT GGAGACCCTG 
CTGCAGCACT TACCCCGGCT GCCCCAGCTA AGCCTGCTGC AGCTAAGCCA GACGAGGCTT 
TCCCCAAAGA GCCTCCTCCT GCTGGCCGAC CTCTTCAGCT TATGCCCCCG GGTTCAGAAG 
GTGGATATCA GGTCCCTGTA TCACATGACA CTGCACTTCA GGTCTGGCGA GGAGCATGAA 
GGCGAGTGCT GTGGCAGGTT CACGGGCTGT GGCCTTAGTC AGGAGCATGT GGAGCCGCTC 
TGCTGGTTGC TGAGCAAATG CGAAGACCTC AACCAGTTGG ACCTCTCAGC AAACATGCTG 
GGTGATGACG GGCTCAGGTG CCTCCTGGAA TGTCTGCCTC AATTGCCCAT CTCTGGTTCG 
CTTGACCTGA GTCACAACAG CATCTCCCTA GAGAGTGCCC TGGGCCTGGT GAAGACACTC 
CCCTCCTGCC CGCACATCCG GGAGGCTTCT GTGAACCTGG GCTCTGAGCA GAGCTTCTGG 
ATCTACTTCT CCCGAAAGGA GGAGGCCGGG AAGACACTAA GGCTGAGCCA GTGCAACCTC 
ATGCCAGAGC ATGTGCCCAG ACTGGCCACC CACCTGAACC AGGCCCTGAA GCTGACAGCG 
CTCACGCTGA CCCAGTGCGG CCTGGGCCTG GAGCAGCTGA TAGTTCTCCT GAGTCTGCTG 
AGATGTCCAG TGGACCTGTT CACTCTCAGG GTGGAGGAGC CGTGGGTAGG CAAAGCCGGG 
GTGCTCGCCC TGCTAAGAGT CTGCACCCAG ACCTCAGGCA ACATCACTGA AATAAGCATT 
TCGGAGACCC AGCAGCAGCT CTGTCTCCAG CTGGAGTTTC CCCCTCAGGA GAACCCAGAA 
GCCGTGACGC TCAGGTTGGC TCACTGTGAC CTTGAGACCC ACCACAGCCT TCTTGCTGGG 
CAGCTGATGG AGACATGTGC CAGGCTGCAG CAGCTCAGCT TGTCCCAGGT GAACCTCTGC 
GACTCCAGCT CCCTGCTGCT AAAAAACCTC CTGGTGTCTC TCTCTGAGCT GAAGACATTC 
AGACTGACCT CCAGCTGTAT GAGCTCCAAG GGCCTGGCCC ACCTGACATT TGGCCTGAGA 
AACTGCCACC ACCTGGAGGA GCTGGACTTG TCCAACAATC AGTTTGGTGA GGAGGACACT 
GAAGTCCTGA TGACGGTCCT TGAGGGCAAG TGCTGGCTGA AGAAGCTCGA CCTCAGCCAT 
CTCCCACTAG GCAGCTCCAC CCTGGCCATG TTCACTCAAG GACTAAGCCA CATGACCCTC 
CTACAGAGCC TCCGGCTGAG CAGGAACGGT ATTTGTGATG TCGGCTGCCA CCAACTTTCC 
GAGGCCCTCA GAGCTGCCAT GAGCTTGAAG GAGCTGGGCT TGAGCCACAA CCAGATCAGA 
GACACCGGTG CCCAGCACTT AGCTGCTGTC CTACCAGGAC TGCCGGAGCT CAGGAAGATA 
GACCTCTCAG GGAATGGCAT TGGTCCAGCT GGAGGGACGC GGCTGGCAGA GTCCCTCACC 
CTCTGCAGGC ACTTGGAGGA GCTGATGCTT GGCTACAACG TCCTGGGGGA TACCACAGCC 
CTGGGCCTGG CCCAGGGACT GCCCTGCTGC CTGAGGGTCC TGCACCTGCC GTCTAGCCGT 
CTGGGCCCAA AGGGAGCGCT GAGCCTGAGC CAGGCCCTGG ATGGATGCCC ACATGTGGAA 
GAGATCAGCT TGGCAGAGAA CAGTCTGGCC AGAGGGGTCC TGCATTTCTG TCAGGGGCTC 
CCCCTGCTGA GGCAGATAGA CCTGGTTTCG TGTGAGATCG ACAACCACAC CGCCAAGCCC 
CTGGCTGCCA GCCTGGTGCT CTGCCCAGCC CTGGAAGAAA TCCTGCTGTC CTGGAATCTG 
CTCGGGGACG AGGCGGCTGC CGAGTTGGCC CGGGTCCTGC CGCAGATGGG CCGGCTGAAG 
AGAATGGACC TGGAGAAGAA TCGGATCACA GCTTGTGGAG CCTGGCTCCT GGCTGAAGGG 
CTGGCACAAG GGTCTGGCAT CCAAGTCATT CGCCTCTGGA ACAATCCCAT CTCCCCCACC 
ATGGTCCAGC ATCTACAGAG CCAGGAGCCC AGGCTGGACT TCGCTTTCTT CGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_544394.5
CDS	331..5910
Translation	MDHVSFQLGTTNLWSWLVRLLSKDPEWLNAKVKFFLPKMDLSSGDEAQDTTQRVMLQLRRLHTQGRATWQAFIHCVCMELDVPLDLEVPLLSTWGHGDGFPSQLGAGEESQPASQLHHGLKRPHQSCGSSPRLKQCKNHQLELARRYLKLLRTSAQQRYSRIPGPGQPLAFHQAYIPPILQWSRTTAPFSAQDGAVVGDPKAEDDTDVSIRDLFSTRADKGPRVTVLLGKAGMGKTTLAHRLCQRWAAGQLDRFQALFLFEFRQLNLITSSVTLPQLLFDLYLSPEAGPDSVFQYLEEHAGGVLLIFDGLDQALHSHSSTEPEGPEAPSPALALFSGLCRGTILPGCWVLATSRPGKLPDCLPTEAATVHMWGFDRPRVEEYVGRFFSDQEWQEAALAELRENRHLQSMCAVPALCQVTCLCLHHLLPGNSPGQSAALLPTVTQNYVQMVSTLSPRGYLPPESLLGLSEMALRGLETGKVIFSVGDISPPMVAFGAALGLLTSFCICTGPGHQGIGYAFTHLSLQEFFAALYLMASPKVDKNVLARHVTLNSRWVLRTKARLGLLDHLPTFLAGLASCACRPFLSHVAQQDEMWVSDKQAAVMLALRKLAIRRLTGPKVVALCHCVGETQEPELASLMAQSLPSHLSFHNFPLTYTDLSALTNILRHRNASIHLDFEGCPLEPHCPEALAGCKQIENLSFKSRKCGDAFAEALSKSLPTMESLKKLGLAGCKITARGISHLMQALRLCPQLEEISFQDNQLKDRAVLNIVEVLPSLPRLQKLDLSRSNVSVSTLLCLTKVAVTCPTVKMLQVRETDLIFLLSPPTETAAELGAAPDLQGNAIQRKEAQSRSLALRLLQCQLGIHDVQELIAQLREGPRLEEVNLSGNQLDDEGCRLMAEAASQLHITRNLDLSNNGLSVDRVHCMLSAVNTCQNLAELHISLLHKTVVFTFAPEQEEQEGIWKRTALRDSLTHQVPFELPLRSTRIRLTHCGLQAKHLEQLCRALGGSCHHSHLDLSGNALGDEGVAQLAQLLPGLGALQSLNLSENGLSLDTVFILIQCFSTLQWLLHLDIGFESQHVVLRGDRRGRDRLAGGCPPEFLAGVPFLAFDQCCVPESFCLRQCQLEPLNLTRLCETLEKCPGPLEVELSFEVLSDQSLETLLQHLPRLPQLSLLQLSQTRLSPKSLLLLADLFSLCPRVQKVDIRSLYHMTLHFRSGEEHEGECCGRFTGCGLSQEHVEPLCWLLSKCEDLNQLDLSANMLGDDGLRCLLECLPQLPISGSLDLSHNSISLESALGLVKTLPSCPHIREASVNLGSEQSFWIYFSRKEEAGKTLRLSQCNLMPEHVPRLATHLNQALKLTALTLTQCGLGLEQLIVLLSLLRCPVDLFTLRVEEPWVGKAGVLALLRVCTQTSGNITEISISETQQQLCLQLEFPPQENPEAVTLRLAHCDLETHHSLLAGQLMETCARLQQLSLSQVNLCDSSSLLLKNLLVSLSELKTFRLTSSCMSSKGLAHLTFGLRNCHHLEELDLSNNQFGEEDTEVLMTVLEGKCWLKKLDLSHLPLGSSTLAMFTQGLSHMTLLQSLRLSRNGICDVGCHQLSEALRAAMSLKELGLSHNQIRDTGAQHLAAVLPGLPELRKIDLSGNGIGPAGGTRLAESLTLCRHLEELMLGYNVLGDTTALGLAQGLPCCLRVLHLPSSRLGPKGALSLSQALDGCPHVEEISLAENSLARGVLHFCQGLPLLRQIDLVSCEIDNHTAKPLAASLVLCPALEEILLSWNLLGDEAAAELARVLPQMGRLKRMDLEKNRITACGAWLLAEGLAQGSGIQVIRLWNNPISPTMVQHLQSQEPRLDFAFFDK

Target ORF information:

RefSeq Version	XM_544394.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NLR family CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_544394.6

ORF Insert Sequence:

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGCTTT 
AAGAGCAGGA AATGTGGGGA TGCCTTTGCA GAAGCCCTCT CCAAGAGCCT GCCAACAATG 
GAGAGCCTGA AGAAGCTCGG GTTAGCAGGA TGTAAGATCA CTGCCCGAGG CATCAGCCAC 
CTGATGCAGG CTTTGCGCCT CTGTCCACAG CTGGAAGAGA TCAGCTTCCA GGACAACCAG 
CTGAAGGATA GGGCCGTGCT GAATATCGTG GAAGTACTTC CTTCTTTGCC ACGGCTCCAG 
AAGCTTGACC TGAGCCGCAG CAATGTCTCC GTGTCAACCT TACTCTGCTT GACAAAGGTG 
GCGGTCACAT GCCCTACTGT CAAGATGCTA CAGGTCAGGG AGACGGACCT CATCTTTCTT 
CTTTCCCCGC CCACAGAGAC TGCTGCAGAG CTTGGAGCAG CACCAGATCT ACAGGGAAAT 
GCCATCCAGA GGAAAGAGGC TCAGAGCAGA AGCCTGGCAC TCAGGCTCCT GCAGTGCCAG 
CTCGGAATTC ATGATGTGCA GGAGCTCATT GCCCAGCTCC GGGAAGGTCC CCGCCTGGAG 
GAAGTAAACC TTTCAGGAAA CCAGCTGGAT GATGAAGGCT GTCGACTGAT GGCAGAAGCT 
GCATCCCAGC TGCACATCAC CAGGAATCTA GACCTCAGCA ACAACGGGCT ATCTGTGGAC 
AGGGTACACT GTATGCTGAG TGCGGTGAAC ACATGCCAGA ACTTGGCAGA GCTTCATATC 
AGCCTGCTGC ATAAAACTGT GGTCTTCACA TTTGCCCCAG AGCAGGAGGA GCAGGAGGGG 
ATCTGGAAGA GGACTGCGCT TCGGGACAGC CTCACGCACC AGGTGCCCTT TGAGCTGCCC 
CTGCGCTCCA CGAGGATCAG GCTGACACAC TGTGGCTTGC AAGCCAAGCA CCTAGAGCAG 
CTCTGCAGGG CCCTGGGAGG AAGCTGCCAC CACAGTCACC TGGACCTCTC AGGCAATGCT 
CTGGGGGATG AAGGCGTGGC CCAGCTGGCT CAGCTGCTCC CAGGGCTGGG TGCTCTGCAG 
TCCTTGAACC TCAGTGAGAA TGGTTTGTCC CTGGATACAG TGTTTATTTT GATCCAGTGC 
TTCTCGACTC TGCAGTGGCT TCTTCACTTG GATATCGGCT TCGAGAGCCA GCACGTCGTC 
CTGAGAGGTG ACAGAAGAGG CAGGGATCGA TTGGCTGGTG GATGTCCGCC AGAGTTCCTA 
GCTGGAGTCC CATTCTTGGC CTTTGATCAG TGCTGCGTCC CCGAGAGCTT CTGCCTCAGG 
CAATGTCAGC TGGAGCCCCT GAACCTCACC CGCCTCTGCG AGACTCTGGA GAAGTGCCCC 
GGGCCTCTGG AAGTCGAATT GTCCTTTGAG GTCCTGAGCG ACCAGAGCCT GGAGACCCTG 
CTGCAGCACT TACCCCGGCT GCCCCAGCTA AGCCTGCTGC AGCTAAGCCA GACGAGGCTT 
TCCCCAAAGA GCCTCCTCCT GCTGGCCGAC CTCTTCAGCT TATGCCCCCG GGTTCAGAAG 
GTGGATATCA GGTCCCTGTA TCACATGACA CTGCACTTCA GGTCTGGCGA GGAGCATGAA 
GGCGAGTGCT GTGGCAGGTT CACGGGCTGT GGCCTTAGTC AGGAGCATGT GGAGCCGCTC 
TGCTGGTTGC TGAGCAAATG CGAAGACCTC AACCAGTTGG ACCTCTCAGC AAACATGCTG 
GGTGATGACG GGCTCAGGTG CCTCCTGGAA TGTCTGCCTC AATTGCCCAT CTCTGGTTCG 
CTTGACCTGA GTCACAACAG CATCTCCCTA GAGAGTGCCC TGGGCCTGGT GAAGACACTC 
CCCTCCTGCC CGCACATCCG GGAGGCTTCT GTGAACCTGG GCTCTGAGCA GAGCTTCTGG 
ATCTACTTCT CCCGAAAGGA GGAGGCCGGG AAGACACTAA GGCTGAGCCA GTGCAACCTC 
ATGCCAGAGC ATGTGCCCAG ACTGGCCACC CACCTGAACC AGGCCCTGAA GCTGACAGCG 
CTCACGCTGA CCCAGTGCGG CCTGGGCCTG GAGCAGCTGA TAGTTCTCCT GAGTCTGCTG 
AGATGTCCAG TGGACCTGTT CACTCTCAGG GTGGAGGAGC CGTGGGTAGG CAAAGCCGGG 
GTGCTCGCCC TGCTAAGAGT CTGCACCCAG ACCTCAGGCA ACATCACTGA AATAAGCATT 
TCGGAGACCC AGCAGCAGCT CTGTCTCCAG CTGGAGTTTC CCCCTCAGGA GAACCCAGAA 
GCCGTGACGC TCAGGTTGGC TCACTGTGAC CTTGAGACCC ACCACAGCCT TCTTGCTGGG 
CAGCTGATGG AGACATGTGC CAGGCTGCAG CAGCTCAGCT TGTCCCAGGT GAACCTCTGC 
GACTCCAGCT CCCTGCTGCT AAAAAACCTC CTGGTGTCTC TCTCTGAGCT GAAGACATTC 
AGACTGACCT CCAGCTGTAT GAGCTCCAAG GGCCTGGCCC ACCTGACATT TGGCCTGAGA 
AACTGCCACC ACCTGGAGGA GCTGGACTTG TCCAACAATC AGTTTGGTGA GGAGGACACT 
GAAGTCCTGA TGACGGTCCT TGAGGGCAAG TGCTGGCTGA AGAAGCTCGA CCTCAGCCAT 
CTCCCACTAG GCAGCTCCAC CCTGGCCATG TTCACTCAAG GACTAAGCCA CATGACCCTC 
CTACAGAGCC TCCGGCTGAG CAGGAACGGT ATTTGTGATG TCGGCTGCCA CCAACTTTCC 
GAGGCCCTCA GAGCTGCCAT GAGCTTGAAG GAGCTGGGCT TGAGCCACAA CCAGATCAGA 
GACACCGGTG CCCAGCACTT AGCTGCTGTC CTACCAGGAC TGCCGGAGCT CAGGAAGATA 
GACCTCTCAG GGAATGGCAT TGGTCCAGCT GGAGGGACGC GGCTGGCAGA GTCCCTCACC 
CTCTGCAGGC ACTTGGAGGA GCTGATGCTT GGCTACAACG TCCTGGGGGA TACCACAGCC 
CTGGGCCTGG CCCAGGGACT GCCCTGCTGC CTGAGGGTCC TGCACCTGCC GTCTAGCCGT 
CTGGGCCCAA AGGGAGCGCT GAGCCTGAGC CAGGCCCTGG ATGGATGCCC ACATGTGGAA 
GAGATCAGCT TGGCAGAGAA CAGTCTGGCC AGAGGGGTCC TGCATTTCTG TCAGGGGCTC 
CCCCTGCTGA GGCAGATAGA CCTGGTTTCG TGTGAGATCG ACAACCACAC CGCCAAGCCC 
CTGGCTGCCA GCCTGGTGCT CTGCCCAGCC CTGGAAGAAA TCCTGCTGTC CTGGAATCTG 
CTCGGGGACG AGGCGGCTGC CGAGTTGGCC CGGGTCCTGC CGCAGATGGG CCGGCTGAAG 
AGAATGGACC TGGAGAAGAA TCGGATCACA GCTTGTGGAG CCTGGCTCCT GGCTGAAGGG 
CTGGCACAAG GGTCTGGCAT CCAAGTCATT CGCCTCTGGA ACAATCCCAT CTCCCCCACC 
ATGGTCCAGC ATCTACAGAG CCAGGAGCCC AGGCTGGACT TCGCTTTCTT CGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_014109333.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964808.1 Canis lupus familiaris NLR family CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.

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CloneID	OCe35403
Clone ID Related Accession (Same CDS sequence)	XM_544394.5 , XM_014109333.1 , XM_544394.6 , XM_014109333.2
Accession Version	XM_014109333.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein NLRC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGCTTT 
AAGAGCAGGA AATGTGGGGA TGCCTTTGCA GAAGCCCTCT CCAAGAGCCT GCCAACAATG 
GAGAGCCTGA AGAAGCTCGG GTTAGCAGGA TGTAAGATCA CTGCCCGAGG CATCAGCCAC 
CTGATGCAGG CTTTGCGCCT CTGTCCACAG CTGGAAGAGA TCAGCTTCCA GGACAACCAG 
CTGAAGGATA GGGCCGTGCT GAATATCGTG GAAGTACTTC CTTCTTTGCC ACGGCTCCAG 
AAGCTTGACC TGAGCCGCAG CAATGTCTCC GTGTCAACCT TACTCTGCTT GACAAAGGTG 
GCGGTCACAT GCCCTACTGT CAAGATGCTA CAGGTCAGGG AGACGGACCT CATCTTTCTT 
CTTTCCCCGC CCACAGAGAC TGCTGCAGAG CTTGGAGCAG CACCAGATCT ACAGGGAAAT 
GCCATCCAGA GGAAAGAGGC TCAGAGCAGA AGCCTGGCAC TCAGGCTCCT GCAGTGCCAG 
CTCGGAATTC ATGATGTGCA GGAGCTCATT GCCCAGCTCC GGGAAGGTCC CCGCCTGGAG 
GAAGTAAACC TTTCAGGAAA CCAGCTGGAT GATGAAGGCT GTCGACTGAT GGCAGAAGCT 
GCATCCCAGC TGCACATCAC CAGGAATCTA GACCTCAGCA ACAACGGGCT ATCTGTGGAC 
AGGGTACACT GTATGCTGAG TGCGGTGAAC ACATGCCAGA ACTTGGCAGA GCTTCATATC 
AGCCTGCTGC ATAAAACTGT GGTCTTCACA TTTGCCCCAG AGCAGGAGGA GCAGGAGGGG 
ATCTGGAAGA GGACTGCGCT TCGGGACAGC CTCACGCACC AGGTGCCCTT TGAGCTGCCC 
CTGCGCTCCA CGAGGATCAG GCTGACACAC TGTGGCTTGC AAGCCAAGCA CCTAGAGCAG 
CTCTGCAGGG CCCTGGGAGG AAGCTGCCAC CACAGTCACC TGGACCTCTC AGGCAATGCT 
CTGGGGGATG AAGGCGTGGC CCAGCTGGCT CAGCTGCTCC CAGGGCTGGG TGCTCTGCAG 
TCCTTGAACC TCAGTGAGAA TGGTTTGTCC CTGGATACAG TGTTTATTTT GATCCAGTGC 
TTCTCGACTC TGCAGTGGCT TCTTCACTTG GATATCGGCT TCGAGAGCCA GCACGTCGTC 
CTGAGAGGTG ACAGAAGAGG CAGGGATCGA TTGGCTGGTG GATGTCCGCC AGAGTTCCTA 
GCTGGAGTCC CATTCTTGGC CTTTGATCAG TGCTGCGTCC CCGAGAGCTT CTGCCTCAGG 
CAATGTCAGC TGGAGCCCCT GAACCTCACC CGCCTCTGCG AGACTCTGGA GAAGTGCCCC 
GGGCCTCTGG AAGTCGAATT GTCCTTTGAG GTCCTGAGCG ACCAGAGCCT GGAGACCCTG 
CTGCAGCACT TACCCCGGCT GCCCCAGCTA AGCCTGCTGC AGCTAAGCCA GACGAGGCTT 
TCCCCAAAGA GCCTCCTCCT GCTGGCCGAC CTCTTCAGCT TATGCCCCCG GGTTCAGAAG 
GTGGATATCA GGTCCCTGTA TCACATGACA CTGCACTTCA GGTCTGGCGA GGAGCATGAA 
GGCGAGTGCT GTGGCAGGTT CACGGGCTGT GGCCTTAGTC AGGAGCATGT GGAGCCGCTC 
TGCTGGTTGC TGAGCAAATG CGAAGACCTC AACCAGTTGG ACCTCTCAGC AAACATGCTG 
GGTGATGACG GGCTCAGGTG CCTCCTGGAA TGTCTGCCTC AATTGCCCAT CTCTGGTTCG 
CTTGACCTGA GTCACAACAG CATCTCCCTA GAGAGTGCCC TGGGCCTGGT GAAGACACTC 
CCCTCCTGCC CGCACATCCG GGAGGCTTCT GTGAACCTGG GCTCTGAGCA GAGCTTCTGG 
ATCTACTTCT CCCGAAAGGA GGAGGCCGGG AAGACACTAA GGCTGAGCCA GTGCAACCTC 
ATGCCAGAGC ATGTGCCCAG ACTGGCCACC CACCTGAACC AGGCCCTGAA GCTGACAGCG 
CTCACGCTGA CCCAGTGCGG CCTGGGCCTG GAGCAGCTGA TAGTTCTCCT GAGTCTGCTG 
AGATGTCCAG TGGACCTGTT CACTCTCAGG GTGGAGGAGC CGTGGGTAGG CAAAGCCGGG 
GTGCTCGCCC TGCTAAGAGT CTGCACCCAG ACCTCAGGCA ACATCACTGA AATAAGCATT 
TCGGAGACCC AGCAGCAGCT CTGTCTCCAG CTGGAGTTTC CCCCTCAGGA GAACCCAGAA 
GCCGTGACGC TCAGGTTGGC TCACTGTGAC CTTGAGACCC ACCACAGCCT TCTTGCTGGG 
CAGCTGATGG AGACATGTGC CAGGCTGCAG CAGCTCAGCT TGTCCCAGGT GAACCTCTGC 
GACTCCAGCT CCCTGCTGCT AAAAAACCTC CTGGTGTCTC TCTCTGAGCT GAAGACATTC 
AGACTGACCT CCAGCTGTAT GAGCTCCAAG GGCCTGGCCC ACCTGACATT TGGCCTGAGA 
AACTGCCACC ACCTGGAGGA GCTGGACTTG TCCAACAATC AGTTTGGTGA GGAGGACACT 
GAAGTCCTGA TGACGGTCCT TGAGGGCAAG TGCTGGCTGA AGAAGCTCGA CCTCAGCCAT 
CTCCCACTAG GCAGCTCCAC CCTGGCCATG TTCACTCAAG GACTAAGCCA CATGACCCTC 
CTACAGAGCC TCCGGCTGAG CAGGAACGGT ATTTGTGATG TCGGCTGCCA CCAACTTTCC 
GAGGCCCTCA GAGCTGCCAT GAGCTTGAAG GAGCTGGGCT TGAGCCACAA CCAGATCAGA 
GACACCGGTG CCCAGCACTT AGCTGCTGTC CTACCAGGAC TGCCGGAGCT CAGGAAGATA 
GACCTCTCAG GGAATGGCAT TGGTCCAGCT GGAGGGACGC GGCTGGCAGA GTCCCTCACC 
CTCTGCAGGC ACTTGGAGGA GCTGATGCTT GGCTACAACG TCCTGGGGGA TACCACAGCC 
CTGGGCCTGG CCCAGGGACT GCCCTGCTGC CTGAGGGTCC TGCACCTGCC GTCTAGCCGT 
CTGGGCCCAA AGGGAGCGCT GAGCCTGAGC CAGGCCCTGG ATGGATGCCC ACATGTGGAA 
GAGATCAGCT TGGCAGAGAA CAGTCTGGCC AGAGGGGTCC TGCATTTCTG TCAGGGGCTC 
CCCCTGCTGA GGCAGATAGA CCTGGTTTCG TGTGAGATCG ACAACCACAC CGCCAAGCCC 
CTGGCTGCCA GCCTGGTGCT CTGCCCAGCC CTGGAAGAAA TCCTGCTGTC CTGGAATCTG 
CTCGGGGACG AGGCGGCTGC CGAGTTGGCC CGGGTCCTGC CGCAGATGGG CCGGCTGAAG 
AGAATGGACC TGGAGAAGAA TCGGATCACA GCTTGTGGAG CCTGGCTCCT GGCTGAAGGG 
CTGGCACAAG GGTCTGGCAT CCAAGTCATT CGCCTCTGGA ACAATCCCAT CTCCCCCACC 
ATGGTCCAGC ATCTACAGAG CCAGGAGCCC AGGCTGGACT TCGCTTTCTT CGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964808.1
CDS	229..5808
Translation	MDHVSFQLGTTNLWSWLVRLLSKDPEWLNAKVKFFLPKMDLSSGDEAQDTTQRVMLQLRRLHTQGRATWQAFIHCVCMELDVPLDLEVPLLSTWGHGDGFPSQLGAGEESQPASQLHHGLKRPHQSCGSSPRLKQCKNHQLELARRYLKLLRTSAQQRYSRIPGPGQPLAFHQAYIPPILQWSRTTAPFSAQDGAVVGDPKAEDDTDVSIRDLFSTRADKGPRVTVLLGKAGMGKTTLAHRLCQRWAAGQLDRFQALFLFEFRQLNLITSSVTLPQLLFDLYLSPEAGPDSVFQYLEEHAGGVLLIFDGLDQALHSHSSTEPEGPEAPSPALALFSGLCRGTILPGCWVLATSRPGKLPDCLPTEAATVHMWGFDRPRVEEYVGRFFSDQEWQEAALAELRENRHLQSMCAVPALCQVTCLCLHHLLPGNSPGQSAALLPTVTQNYVQMVSTLSPRGYLPPESLLGLSEMALRGLETGKVIFSVGDISPPMVAFGAALGLLTSFCICTGPGHQGIGYAFTHLSLQEFFAALYLMASPKVDKNVLARHVTLNSRWVLRTKARLGLLDHLPTFLAGLASCACRPFLSHVAQQDEMWVSDKQAAVMLALRKLAIRRLTGPKVVALCHCVGETQEPELASLMAQSLPSHLSFHNFPLTYTDLSALTNILRHRNASIHLDFEGCPLEPHCPEALAGCKQIENLSFKSRKCGDAFAEALSKSLPTMESLKKLGLAGCKITARGISHLMQALRLCPQLEEISFQDNQLKDRAVLNIVEVLPSLPRLQKLDLSRSNVSVSTLLCLTKVAVTCPTVKMLQVRETDLIFLLSPPTETAAELGAAPDLQGNAIQRKEAQSRSLALRLLQCQLGIHDVQELIAQLREGPRLEEVNLSGNQLDDEGCRLMAEAASQLHITRNLDLSNNGLSVDRVHCMLSAVNTCQNLAELHISLLHKTVVFTFAPEQEEQEGIWKRTALRDSLTHQVPFELPLRSTRIRLTHCGLQAKHLEQLCRALGGSCHHSHLDLSGNALGDEGVAQLAQLLPGLGALQSLNLSENGLSLDTVFILIQCFSTLQWLLHLDIGFESQHVVLRGDRRGRDRLAGGCPPEFLAGVPFLAFDQCCVPESFCLRQCQLEPLNLTRLCETLEKCPGPLEVELSFEVLSDQSLETLLQHLPRLPQLSLLQLSQTRLSPKSLLLLADLFSLCPRVQKVDIRSLYHMTLHFRSGEEHEGECCGRFTGCGLSQEHVEPLCWLLSKCEDLNQLDLSANMLGDDGLRCLLECLPQLPISGSLDLSHNSISLESALGLVKTLPSCPHIREASVNLGSEQSFWIYFSRKEEAGKTLRLSQCNLMPEHVPRLATHLNQALKLTALTLTQCGLGLEQLIVLLSLLRCPVDLFTLRVEEPWVGKAGVLALLRVCTQTSGNITEISISETQQQLCLQLEFPPQENPEAVTLRLAHCDLETHHSLLAGQLMETCARLQQLSLSQVNLCDSSSLLLKNLLVSLSELKTFRLTSSCMSSKGLAHLTFGLRNCHHLEELDLSNNQFGEEDTEVLMTVLEGKCWLKKLDLSHLPLGSSTLAMFTQGLSHMTLLQSLRLSRNGICDVGCHQLSEALRAAMSLKELGLSHNQIRDTGAQHLAAVLPGLPELRKIDLSGNGIGPAGGTRLAESLTLCRHLEELMLGYNVLGDTTALGLAQGLPCCLRVLHLPSSRLGPKGALSLSQALDGCPHVEEISLAENSLARGVLHFCQGLPLLRQIDLVSCEIDNHTAKPLAASLVLCPALEEILLSWNLLGDEAAAELARVLPQMGRLKRMDLEKNRITACGAWLLAEGLAQGSGIQVIRLWNNPISPTMVQHLQSQEPRLDFAFFDK

Target ORF information:

RefSeq Version	XM_014109333.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NLR family CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109333.2

ORF Insert Sequence:

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGCTTT 
AAGAGCAGGA AATGTGGGGA TGCCTTTGCA GAAGCCCTCT CCAAGAGCCT GCCAACAATG 
GAGAGCCTGA AGAAGCTCGG GTTAGCAGGA TGTAAGATCA CTGCCCGAGG CATCAGCCAC 
CTGATGCAGG CTTTGCGCCT CTGTCCACAG CTGGAAGAGA TCAGCTTCCA GGACAACCAG 
CTGAAGGATA GGGCCGTGCT GAATATCGTG GAAGTACTTC CTTCTTTGCC ACGGCTCCAG 
AAGCTTGACC TGAGCCGCAG CAATGTCTCC GTGTCAACCT TACTCTGCTT GACAAAGGTG 
GCGGTCACAT GCCCTACTGT CAAGATGCTA CAGGTCAGGG AGACGGACCT CATCTTTCTT 
CTTTCCCCGC CCACAGAGAC TGCTGCAGAG CTTGGAGCAG CACCAGATCT ACAGGGAAAT 
GCCATCCAGA GGAAAGAGGC TCAGAGCAGA AGCCTGGCAC TCAGGCTCCT GCAGTGCCAG 
CTCGGAATTC ATGATGTGCA GGAGCTCATT GCCCAGCTCC GGGAAGGTCC CCGCCTGGAG 
GAAGTAAACC TTTCAGGAAA CCAGCTGGAT GATGAAGGCT GTCGACTGAT GGCAGAAGCT 
GCATCCCAGC TGCACATCAC CAGGAATCTA GACCTCAGCA ACAACGGGCT ATCTGTGGAC 
AGGGTACACT GTATGCTGAG TGCGGTGAAC ACATGCCAGA ACTTGGCAGA GCTTCATATC 
AGCCTGCTGC ATAAAACTGT GGTCTTCACA TTTGCCCCAG AGCAGGAGGA GCAGGAGGGG 
ATCTGGAAGA GGACTGCGCT TCGGGACAGC CTCACGCACC AGGTGCCCTT TGAGCTGCCC 
CTGCGCTCCA CGAGGATCAG GCTGACACAC TGTGGCTTGC AAGCCAAGCA CCTAGAGCAG 
CTCTGCAGGG CCCTGGGAGG AAGCTGCCAC CACAGTCACC TGGACCTCTC AGGCAATGCT 
CTGGGGGATG AAGGCGTGGC CCAGCTGGCT CAGCTGCTCC CAGGGCTGGG TGCTCTGCAG 
TCCTTGAACC TCAGTGAGAA TGGTTTGTCC CTGGATACAG TGTTTATTTT GATCCAGTGC 
TTCTCGACTC TGCAGTGGCT TCTTCACTTG GATATCGGCT TCGAGAGCCA GCACGTCGTC 
CTGAGAGGTG ACAGAAGAGG CAGGGATCGA TTGGCTGGTG GATGTCCGCC AGAGTTCCTA 
GCTGGAGTCC CATTCTTGGC CTTTGATCAG TGCTGCGTCC CCGAGAGCTT CTGCCTCAGG 
CAATGTCAGC TGGAGCCCCT GAACCTCACC CGCCTCTGCG AGACTCTGGA GAAGTGCCCC 
GGGCCTCTGG AAGTCGAATT GTCCTTTGAG GTCCTGAGCG ACCAGAGCCT GGAGACCCTG 
CTGCAGCACT TACCCCGGCT GCCCCAGCTA AGCCTGCTGC AGCTAAGCCA GACGAGGCTT 
TCCCCAAAGA GCCTCCTCCT GCTGGCCGAC CTCTTCAGCT TATGCCCCCG GGTTCAGAAG 
GTGGATATCA GGTCCCTGTA TCACATGACA CTGCACTTCA GGTCTGGCGA GGAGCATGAA 
GGCGAGTGCT GTGGCAGGTT CACGGGCTGT GGCCTTAGTC AGGAGCATGT GGAGCCGCTC 
TGCTGGTTGC TGAGCAAATG CGAAGACCTC AACCAGTTGG ACCTCTCAGC AAACATGCTG 
GGTGATGACG GGCTCAGGTG CCTCCTGGAA TGTCTGCCTC AATTGCCCAT CTCTGGTTCG 
CTTGACCTGA GTCACAACAG CATCTCCCTA GAGAGTGCCC TGGGCCTGGT GAAGACACTC 
CCCTCCTGCC CGCACATCCG GGAGGCTTCT GTGAACCTGG GCTCTGAGCA GAGCTTCTGG 
ATCTACTTCT CCCGAAAGGA GGAGGCCGGG AAGACACTAA GGCTGAGCCA GTGCAACCTC 
ATGCCAGAGC ATGTGCCCAG ACTGGCCACC CACCTGAACC AGGCCCTGAA GCTGACAGCG 
CTCACGCTGA CCCAGTGCGG CCTGGGCCTG GAGCAGCTGA TAGTTCTCCT GAGTCTGCTG 
AGATGTCCAG TGGACCTGTT CACTCTCAGG GTGGAGGAGC CGTGGGTAGG CAAAGCCGGG 
GTGCTCGCCC TGCTAAGAGT CTGCACCCAG ACCTCAGGCA ACATCACTGA AATAAGCATT 
TCGGAGACCC AGCAGCAGCT CTGTCTCCAG CTGGAGTTTC CCCCTCAGGA GAACCCAGAA 
GCCGTGACGC TCAGGTTGGC TCACTGTGAC CTTGAGACCC ACCACAGCCT TCTTGCTGGG 
CAGCTGATGG AGACATGTGC CAGGCTGCAG CAGCTCAGCT TGTCCCAGGT GAACCTCTGC 
GACTCCAGCT CCCTGCTGCT AAAAAACCTC CTGGTGTCTC TCTCTGAGCT GAAGACATTC 
AGACTGACCT CCAGCTGTAT GAGCTCCAAG GGCCTGGCCC ACCTGACATT TGGCCTGAGA 
AACTGCCACC ACCTGGAGGA GCTGGACTTG TCCAACAATC AGTTTGGTGA GGAGGACACT 
GAAGTCCTGA TGACGGTCCT TGAGGGCAAG TGCTGGCTGA AGAAGCTCGA CCTCAGCCAT 
CTCCCACTAG GCAGCTCCAC CCTGGCCATG TTCACTCAAG GACTAAGCCA CATGACCCTC 
CTACAGAGCC TCCGGCTGAG CAGGAACGGT ATTTGTGATG TCGGCTGCCA CCAACTTTCC 
GAGGCCCTCA GAGCTGCCAT GAGCTTGAAG GAGCTGGGCT TGAGCCACAA CCAGATCAGA 
GACACCGGTG CCCAGCACTT AGCTGCTGTC CTACCAGGAC TGCCGGAGCT CAGGAAGATA 
GACCTCTCAG GGAATGGCAT TGGTCCAGCT GGAGGGACGC GGCTGGCAGA GTCCCTCACC 
CTCTGCAGGC ACTTGGAGGA GCTGATGCTT GGCTACAACG TCCTGGGGGA TACCACAGCC 
CTGGGCCTGG CCCAGGGACT GCCCTGCTGC CTGAGGGTCC TGCACCTGCC GTCTAGCCGT 
CTGGGCCCAA AGGGAGCGCT GAGCCTGAGC CAGGCCCTGG ATGGATGCCC ACATGTGGAA 
GAGATCAGCT TGGCAGAGAA CAGTCTGGCC AGAGGGGTCC TGCATTTCTG TCAGGGGCTC 
CCCCTGCTGA GGCAGATAGA CCTGGTTTCG TGTGAGATCG ACAACCACAC CGCCAAGCCC 
CTGGCTGCCA GCCTGGTGCT CTGCCCAGCC CTGGAAGAAA TCCTGCTGTC CTGGAATCTG 
CTCGGGGACG AGGCGGCTGC CGAGTTGGCC CGGGTCCTGC CGCAGATGGG CCGGCTGAAG 
AGAATGGACC TGGAGAAGAA TCGGATCACA GCTTGTGGAG CCTGGCTCCT GGCTGAAGGG 
CTGGCACAAG GGTCTGGCAT CCAAGTCATT CGCCTCTGGA ACAATCCCAT CTCCCCCACC 
ATGGTCCAGC ATCTACAGAG CCAGGAGCCC AGGCTGGACT TCGCTTTCTT CGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_014109337.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964812.1 Canis lupus familiaris NLR family, CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.

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CloneID	OCe35404
Clone ID Related Accession (Same CDS sequence)	XM_014109337.1 , XM_014109337.2
Accession Version	XM_014109337.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein NLRC5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGGTTA 
GCAGGATGTA AGATCACTGC CCGAGGCATC AGCCACCTGA TGCAGGCTTT GCGCCTCTGT 
CCACAGCTGG AAGAGATCAG CTTCCAGGAC AACCAGCTGA AGGATAGGGC CGTGCTGAAT 
ATCGTGGAAG TACTTCCTTC TTTGCCACGG CTCCAGAAGC TTGACCTGAG CCGCAGCAAT 
GTCTCCGTGT CAACCTTACT CTGCTTGACA AAGGTGGCGG TCACATGCCC TACTGTCAAG 
ATGCTACAGG TCAGGGAGAC GGACCTCATC TTTCTTCTTT CCCCGCCCAC AGAGACTGCT 
GCAGAGCTTG GAGCAGCACC AGATCTACAG GGAAATGCCA TCCAGAGGAA AGAGGCTCAG 
AGCAGAAGCC TGGCACTCAG GCTCCTGCAG TGCCAGCTCG GAATTCATGA TGTGCAGGAG 
CTCATTGCCC AGCTCCGGGA AGGTCCCCGC CTGGAGGAAG TAAACCTTTC AGGAAACCAG 
CTGGATGATG AAGGCTGTCG ACTGATGGCA GAAGCTGCAT CCCAGCTGCA CATCACCAGG 
AATCTAGACC TCAGCAACAA CGGGCTATCT GTGGACAGGG TACACTGTAT GCTGAGTGCG 
GTGAACACAT GCCAGAACTT GGCAGAGCTT CATATCAGCC TGCTGCATAA AACTGTGGTC 
TTCACATTTG CCCCAGAGCA GGAGGAGCAG GAGGGGATCT GGAAGAGGAC TGCGCTTCGG 
GACAGCCTCA CGCACCAGGT GCCCTTTGAG CTGCCCCTGC GCTCCACGAG GATCAGGCTG 
ACACACTGTG GCTTGCAAGC CAAGCACCTA GAGCAGCTCT GCAGGGCCCT GGGAGGAAGC 
TGCCACCACA GTCACCTGGA CCTCTCAGGC AATGCTCTGG GGGATGAAGG CGTGGCCCAG 
CTGGCTCAGC TGCTCCCAGG GCTGGGTGCT CTGCAGTCCT TGAACCTCAG TGAGAATGGT 
TTGTCCCTGG ATACAGTGTT TATTTTGATC CAGTGCTTCT CGACTCTGCA GTGGCTTCTT 
CACTTGGATA TCGGCTTCGA GAGCCAGCAC GTCGTCCTGA GAGGTGACAG AAGAGGCAGG 
GATCGATTGG CTGGTGGATG TCCGCCAGAG TTCCTAGCTG GAGTCCCATT CTTGGCCTTT 
GATCAGTGCT GCGTCCCCGA GAGCTTCTGC CTCAGGCAAT GTCAGCTGGA GCCCCTGAAC 
CTCACCCGCC TCTGCGAGAC TCTGGAGAAG TGCCCCGGGC CTCTGGAAGT CGAATTGTCC 
TTTGAGGTCC TGAGCGACCA GAGCCTGGAG ACCCTGCTGC AGCACTTACC CCGGCTGCCC 
CAGCTAAGCC TGCTGCAGCT AAGCCAGACG AGGCTTTCCC CAAAGAGCCT CCTCCTGCTG 
GCCGACCTCT TCAGCTTATG CCCCCGGGTT CAGAAGGTGG ATATCAGGTC CCTGTATCAC 
ATGACACTGC ACTTCAGGTC TGGCGAGGAG CATGAAGGCG AGTGCTGTGG CAGGTTCACG 
GGCTGTGGCC TTAGTCAGGA GCATGTGGAG CCGCTCTGCT GGTTGCTGAG CAAATGCGAA 
GACCTCAACC AGTTGGACCT CTCAGCAAAC ATGCTGGGTG ATGACGGGCT CAGGTGCCTC 
CTGGAATGTC TGCCTCAATT GCCCATCTCT GGTTCGCTTG ACCTGAGTCA CAACAGCATC 
TCCCTAGAGA GTGCCCTGGG CCTGGTGAAG ACACTCCCCT CCTGCCCGCA CATCCGGGAG 
GCTTCTGTGA ACCTGGGCTC TGAGCAGAGC TTCTGGATCT ACTTCTCCCG AAAGGAGGAG 
GCCGGGAAGA CACTAAGGCT GAGCCAGTGC AACCTCATGC CAGAGCATGT GCCCAGACTG 
GCCACCCACC TGAACCAGGC CCTGAAGCTG ACAGCGCTCA CGCTGACCCA GTGCGGCCTG 
GGCCTGGAGC AGCTGATAGT TCTCCTGAGT CTGCTGAGAT GTCCAGTGGA CCTGTTCACT 
CTCAGGGTGG AGGAGCCGTG GGTAGGCAAA GCCGGGGTGC TCGCCCTGCT AAGAGTCTGC 
ACCCAGACCT CAGGCAACAT CACTGAAATA AGCATTTCGG AGACCCAGCA GCAGCTCTGT 
CTCCAGCTGG AGTTTCCCCC TCAGGAGAAC CCAGAAGCCG TGACGCTCAG GTTGGCTCAC 
TGTGACCTTG AGACCCACCA CAGCCTTCTT GCTGGGCAGC TGATGGAGAC ATGTGCCAGG 
CTGCAGCAGC TCAGCTTGTC CCAGGTGAAC CTCTGCGACT CCAGCTCCCT GCTGCTAAAA 
AACCTCCTGG TGTCTCTCTC TGAGCTGAAG ACATTCAGAC TGACCTCCAG CTGTATGAGC 
TCCAAGGGCC TGGCCCACCT GACATTTGGC CTGAGAAACT GCCACCACCT GGAGGAGCTG 
GACTTGTCCA ACAATCAGTT TGGTGAGGAG GACACTGAAG TCCTGATGAC GGTCCTTGAG 
GGCAAGTGCT GGCTGAAGAA GCTCGACCTC AGCCATCTCC CACTAGGCAG CTCCACCCTG 
GCCATGTTCA CTCAAGGACT AAGCCACATG ACCCTCCTAC AGAGCCTCCG GCTGAGCAGG 
AACGGTATTT GTGATGTCGG CTGCCACCAA CTTTCCGAGG CCCTCAGAGC TGCCATGAGC 
TTGAAGGAGC TGGGCTTGAG CCACAACCAG ATCAGAGACA CCGGTGCCCA GCACTTAGCT 
GCTGTCCTAC CAGGACTGCC GGAGCTCAGG AAGATAGACC TCTCAGGGAA TGGCATTGGT 
CCAGCTGGAG GGACGCGGCT GGCAGAGTCC CTCACCCTCT GCAGGCACTT GGAGGAGCTG 
ATGCTTGGCT ACAACGTCCT GGGGGATACC ACAGCCCTGG GCCTGGCCCA GGGACTGCCC 
TGCTGCCTGA GGGTCCTGCA CCTGCCGTCT AGCCGTCTGG GCCCAAAGGG AGCGCTGAGC 
CTGAGCCAGG CCCTGGATGG ATGCCCACAT GTGGAAGAGA TCAGCTTGGC AGAGAACAGT 
CTGGCCAGAG GGGTCCTGCA TTTCTGTCAG GGGCTCCCCC TGCTGAGGCA GATAGACCTG 
GTTTCGTGTG AGATCGACAA CCACACCGCC AAGCCCCTGG CTGCCAGCCT GGTGCTCTGC 
CCAGCCCTGG AAGAAATCCT GCTGTCCTGG AATCTGCTCG GGGACGAGGC GGCTGCCGAG 
TTGGCCCGGG TCCTGCCGCA GATGGGCCGG CTGAAGAGAA TGGACCTGGA GAAGAATCGG 
ATCACAGCTT GTGGAGCCTG GCTCCTGGCT GAAGGGCTGG CACAAGGGTC TGGCATCCAA 
GTCATTCGCC TCTGGAACAA TCCCATCTCC CCCACCATGG TCCAGCATCT ACAGAGCCAG 
GAGCCCAGGC TGGACTTCGC TTTCTTCGAC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964812.1
CDS	217..5712
Translation	MDHVSFQLGTTNLWSWLVRLLSKDPEWLNAKVKFFLPKMDLSSGDEAQDTTQRVMLQLRRLHTQGRATWQAFIHCVCMELDVPLDLEVPLLSTWGHGDGFPSQLGAGEESQPASQLHHGLKRPHQSCGSSPRLKQCKNHQLELARRYLKLLRTSAQQRYSRIPGPGQPLAFHQAYIPPILQWSRTTAPFSAQDGAVVGDPKAEDDTDVSIRDLFSTRADKGPRVTVLLGKAGMGKTTLAHRLCQRWAAGQLDRFQALFLFEFRQLNLITSSVTLPQLLFDLYLSPEAGPDSVFQYLEEHAGGVLLIFDGLDQALHSHSSTEPEGPEAPSPALALFSGLCRGTILPGCWVLATSRPGKLPDCLPTEAATVHMWGFDRPRVEEYVGRFFSDQEWQEAALAELRENRHLQSMCAVPALCQVTCLCLHHLLPGNSPGQSAALLPTVTQNYVQMVSTLSPRGYLPPESLLGLSEMALRGLETGKVIFSVGDISPPMVAFGAALGLLTSFCICTGPGHQGIGYAFTHLSLQEFFAALYLMASPKVDKNVLARHVTLNSRWVLRTKARLGLLDHLPTFLAGLASCACRPFLSHVAQQDEMWVSDKQAAVMLALRKLAIRRLTGPKVVALCHCVGETQEPELASLMAQSLPSHLSFHNFPLTYTDLSALTNILRHRNASIHLDFEGCPLEPHCPEALAGCKQIENLRLAGCKITARGISHLMQALRLCPQLEEISFQDNQLKDRAVLNIVEVLPSLPRLQKLDLSRSNVSVSTLLCLTKVAVTCPTVKMLQVRETDLIFLLSPPTETAAELGAAPDLQGNAIQRKEAQSRSLALRLLQCQLGIHDVQELIAQLREGPRLEEVNLSGNQLDDEGCRLMAEAASQLHITRNLDLSNNGLSVDRVHCMLSAVNTCQNLAELHISLLHKTVVFTFAPEQEEQEGIWKRTALRDSLTHQVPFELPLRSTRIRLTHCGLQAKHLEQLCRALGGSCHHSHLDLSGNALGDEGVAQLAQLLPGLGALQSLNLSENGLSLDTVFILIQCFSTLQWLLHLDIGFESQHVVLRGDRRGRDRLAGGCPPEFLAGVPFLAFDQCCVPESFCLRQCQLEPLNLTRLCETLEKCPGPLEVELSFEVLSDQSLETLLQHLPRLPQLSLLQLSQTRLSPKSLLLLADLFSLCPRVQKVDIRSLYHMTLHFRSGEEHEGECCGRFTGCGLSQEHVEPLCWLLSKCEDLNQLDLSANMLGDDGLRCLLECLPQLPISGSLDLSHNSISLESALGLVKTLPSCPHIREASVNLGSEQSFWIYFSRKEEAGKTLRLSQCNLMPEHVPRLATHLNQALKLTALTLTQCGLGLEQLIVLLSLLRCPVDLFTLRVEEPWVGKAGVLALLRVCTQTSGNITEISISETQQQLCLQLEFPPQENPEAVTLRLAHCDLETHHSLLAGQLMETCARLQQLSLSQVNLCDSSSLLLKNLLVSLSELKTFRLTSSCMSSKGLAHLTFGLRNCHHLEELDLSNNQFGEEDTEVLMTVLEGKCWLKKLDLSHLPLGSSTLAMFTQGLSHMTLLQSLRLSRNGICDVGCHQLSEALRAAMSLKELGLSHNQIRDTGAQHLAAVLPGLPELRKIDLSGNGIGPAGGTRLAESLTLCRHLEELMLGYNVLGDTTALGLAQGLPCCLRVLHLPSSRLGPKGALSLSQALDGCPHVEEISLAENSLARGVLHFCQGLPLLRQIDLVSCEIDNHTAKPLAASLVLCPALEEILLSWNLLGDEAAAELARVLPQMGRLKRMDLEKNRITACGAWLLAEGLAQGSGIQVIRLWNNPISPTMVQHLQSQEPRLDFAFFDK

Target ORF information:

RefSeq Version	XM_014109337.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NLR family, CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109337.1

ORF Insert Sequence:

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGGTTA 
GCAGGATGTA AGATCACTGC CCGAGGCATC AGCCACCTGA TGCAGGCTTT GCGCCTCTGT 
CCACAGCTGG AAGAGATCAG CTTCCAGGAC AACCAGCTGA AGGATAGGGC CGTGCTGAAT 
ATCGTGGAAG TACTTCCTTC TTTGCCACGG CTCCAGAAGC TTGACCTGAG CCGCAGCAAT 
GTCTCCGTGT CAACCTTACT CTGCTTGACA AAGGTGGCGG TCACATGCCC TACTGTCAAG 
ATGCTACAGG TCAGGGAGAC GGACCTCATC TTTCTTCTTT CCCCGCCCAC AGAGACTGCT 
GCAGAGCTTG GAGCAGCACC AGATCTACAG GGAAATGCCA TCCAGAGGAA AGAGGCTCAG 
AGCAGAAGCC TGGCACTCAG GCTCCTGCAG TGCCAGCTCG GAATTCATGA TGTGCAGGAG 
CTCATTGCCC AGCTCCGGGA AGGTCCCCGC CTGGAGGAAG TAAACCTTTC AGGAAACCAG 
CTGGATGATG AAGGCTGTCG ACTGATGGCA GAAGCTGCAT CCCAGCTGCA CATCACCAGG 
AATCTAGACC TCAGCAACAA CGGGCTATCT GTGGACAGGG TACACTGTAT GCTGAGTGCG 
GTGAACACAT GCCAGAACTT GGCAGAGCTT CATATCAGCC TGCTGCATAA AACTGTGGTC 
TTCACATTTG CCCCAGAGCA GGAGGAGCAG GAGGGGATCT GGAAGAGGAC TGCGCTTCGG 
GACAGCCTCA CGCACCAGGT GCCCTTTGAG CTGCCCCTGC GCTCCACGAG GATCAGGCTG 
ACACACTGTG GCTTGCAAGC CAAGCACCTA GAGCAGCTCT GCAGGGCCCT GGGAGGAAGC 
TGCCACCACA GTCACCTGGA CCTCTCAGGC AATGCTCTGG GGGATGAAGG CGTGGCCCAG 
CTGGCTCAGC TGCTCCCAGG GCTGGGTGCT CTGCAGTCCT TGAACCTCAG TGAGAATGGT 
TTGTCCCTGG ATACAGTGTT TATTTTGATC CAGTGCTTCT CGACTCTGCA GTGGCTTCTT 
CACTTGGATA TCGGCTTCGA GAGCCAGCAC GTCGTCCTGA GAGGTGACAG AAGAGGCAGG 
GATCGATTGG CTGGTGGATG TCCGCCAGAG TTCCTAGCTG GAGTCCCATT CTTGGCCTTT 
GATCAGTGCT GCGTCCCCGA GAGCTTCTGC CTCAGGCAAT GTCAGCTGGA GCCCCTGAAC 
CTCACCCGCC TCTGCGAGAC TCTGGAGAAG TGCCCCGGGC CTCTGGAAGT CGAATTGTCC 
TTTGAGGTCC TGAGCGACCA GAGCCTGGAG ACCCTGCTGC AGCACTTACC CCGGCTGCCC 
CAGCTAAGCC TGCTGCAGCT AAGCCAGACG AGGCTTTCCC CAAAGAGCCT CCTCCTGCTG 
GCCGACCTCT TCAGCTTATG CCCCCGGGTT CAGAAGGTGG ATATCAGGTC CCTGTATCAC 
ATGACACTGC ACTTCAGGTC TGGCGAGGAG CATGAAGGCG AGTGCTGTGG CAGGTTCACG 
GGCTGTGGCC TTAGTCAGGA GCATGTGGAG CCGCTCTGCT GGTTGCTGAG CAAATGCGAA 
GACCTCAACC AGTTGGACCT CTCAGCAAAC ATGCTGGGTG ATGACGGGCT CAGGTGCCTC 
CTGGAATGTC TGCCTCAATT GCCCATCTCT GGTTCGCTTG ACCTGAGTCA CAACAGCATC 
TCCCTAGAGA GTGCCCTGGG CCTGGTGAAG ACACTCCCCT CCTGCCCGCA CATCCGGGAG 
GCTTCTGTGA ACCTGGGCTC TGAGCAGAGC TTCTGGATCT ACTTCTCCCG AAAGGAGGAG 
GCCGGGAAGA CACTAAGGCT GAGCCAGTGC AACCTCATGC CAGAGCATGT GCCCAGACTG 
GCCACCCACC TGAACCAGGC CCTGAAGCTG ACAGCGCTCA CGCTGACCCA GTGCGGCCTG 
GGCCTGGAGC AGCTGATAGT TCTCCTGAGT CTGCTGAGAT GTCCAGTGGA CCTGTTCACT 
CTCAGGGTGG AGGAGCCGTG GGTAGGCAAA GCCGGGGTGC TCGCCCTGCT AAGAGTCTGC 
ACCCAGACCT CAGGCAACAT CACTGAAATA AGCATTTCGG AGACCCAGCA GCAGCTCTGT 
CTCCAGCTGG AGTTTCCCCC TCAGGAGAAC CCAGAAGCCG TGACGCTCAG GTTGGCTCAC 
TGTGACCTTG AGACCCACCA CAGCCTTCTT GCTGGGCAGC TGATGGAGAC ATGTGCCAGG 
CTGCAGCAGC TCAGCTTGTC CCAGGTGAAC CTCTGCGACT CCAGCTCCCT GCTGCTAAAA 
AACCTCCTGG TGTCTCTCTC TGAGCTGAAG ACATTCAGAC TGACCTCCAG CTGTATGAGC 
TCCAAGGGCC TGGCCCACCT GACATTTGGC CTGAGAAACT GCCACCACCT GGAGGAGCTG 
GACTTGTCCA ACAATCAGTT TGGTGAGGAG GACACTGAAG TCCTGATGAC GGTCCTTGAG 
GGCAAGTGCT GGCTGAAGAA GCTCGACCTC AGCCATCTCC CACTAGGCAG CTCCACCCTG 
GCCATGTTCA CTCAAGGACT AAGCCACATG ACCCTCCTAC AGAGCCTCCG GCTGAGCAGG 
AACGGTATTT GTGATGTCGG CTGCCACCAA CTTTCCGAGG CCCTCAGAGC TGCCATGAGC 
TTGAAGGAGC TGGGCTTGAG CCACAACCAG ATCAGAGACA CCGGTGCCCA GCACTTAGCT 
GCTGTCCTAC CAGGACTGCC GGAGCTCAGG AAGATAGACC TCTCAGGGAA TGGCATTGGT 
CCAGCTGGAG GGACGCGGCT GGCAGAGTCC CTCACCCTCT GCAGGCACTT GGAGGAGCTG 
ATGCTTGGCT ACAACGTCCT GGGGGATACC ACAGCCCTGG GCCTGGCCCA GGGACTGCCC 
TGCTGCCTGA GGGTCCTGCA CCTGCCGTCT AGCCGTCTGG GCCCAAAGGG AGCGCTGAGC 
CTGAGCCAGG CCCTGGATGG ATGCCCACAT GTGGAAGAGA TCAGCTTGGC AGAGAACAGT 
CTGGCCAGAG GGGTCCTGCA TTTCTGTCAG GGGCTCCCCC TGCTGAGGCA GATAGACCTG 
GTTTCGTGTG AGATCGACAA CCACACCGCC AAGCCCCTGG CTGCCAGCCT GGTGCTCTGC 
CCAGCCCTGG AAGAAATCCT GCTGTCCTGG AATCTGCTCG GGGACGAGGC GGCTGCCGAG 
TTGGCCCGGG TCCTGCCGCA GATGGGCCGG CTGAAGAGAA TGGACCTGGA GAAGAATCGG 
ATCACAGCTT GTGGAGCCTG GCTCCTGGCT GAAGGGCTGG CACAAGGGTC TGGCATCCAA 
GTCATTCGCC TCTGGAACAA TCCCATCTCC CCCACCATGG TCCAGCATCT ACAGAGCCAG 
GAGCCCAGGC TGGACTTCGC TTTCTTCGAC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRC5 ( XM_014109337.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964812.1 Canis lupus familiaris NLR family CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.

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CloneID	OCe35404
Clone ID Related Accession (Same CDS sequence)	XM_014109337.1 , XM_014109337.2
Accession Version	XM_014109337.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein NLRC5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109337.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGGTTA 
GCAGGATGTA AGATCACTGC CCGAGGCATC AGCCACCTGA TGCAGGCTTT GCGCCTCTGT 
CCACAGCTGG AAGAGATCAG CTTCCAGGAC AACCAGCTGA AGGATAGGGC CGTGCTGAAT 
ATCGTGGAAG TACTTCCTTC TTTGCCACGG CTCCAGAAGC TTGACCTGAG CCGCAGCAAT 
GTCTCCGTGT CAACCTTACT CTGCTTGACA AAGGTGGCGG TCACATGCCC TACTGTCAAG 
ATGCTACAGG TCAGGGAGAC GGACCTCATC TTTCTTCTTT CCCCGCCCAC AGAGACTGCT 
GCAGAGCTTG GAGCAGCACC AGATCTACAG GGAAATGCCA TCCAGAGGAA AGAGGCTCAG 
AGCAGAAGCC TGGCACTCAG GCTCCTGCAG TGCCAGCTCG GAATTCATGA TGTGCAGGAG 
CTCATTGCCC AGCTCCGGGA AGGTCCCCGC CTGGAGGAAG TAAACCTTTC AGGAAACCAG 
CTGGATGATG AAGGCTGTCG ACTGATGGCA GAAGCTGCAT CCCAGCTGCA CATCACCAGG 
AATCTAGACC TCAGCAACAA CGGGCTATCT GTGGACAGGG TACACTGTAT GCTGAGTGCG 
GTGAACACAT GCCAGAACTT GGCAGAGCTT CATATCAGCC TGCTGCATAA AACTGTGGTC 
TTCACATTTG CCCCAGAGCA GGAGGAGCAG GAGGGGATCT GGAAGAGGAC TGCGCTTCGG 
GACAGCCTCA CGCACCAGGT GCCCTTTGAG CTGCCCCTGC GCTCCACGAG GATCAGGCTG 
ACACACTGTG GCTTGCAAGC CAAGCACCTA GAGCAGCTCT GCAGGGCCCT GGGAGGAAGC 
TGCCACCACA GTCACCTGGA CCTCTCAGGC AATGCTCTGG GGGATGAAGG CGTGGCCCAG 
CTGGCTCAGC TGCTCCCAGG GCTGGGTGCT CTGCAGTCCT TGAACCTCAG TGAGAATGGT 
TTGTCCCTGG ATACAGTGTT TATTTTGATC CAGTGCTTCT CGACTCTGCA GTGGCTTCTT 
CACTTGGATA TCGGCTTCGA GAGCCAGCAC GTCGTCCTGA GAGGTGACAG AAGAGGCAGG 
GATCGATTGG CTGGTGGATG TCCGCCAGAG TTCCTAGCTG GAGTCCCATT CTTGGCCTTT 
GATCAGTGCT GCGTCCCCGA GAGCTTCTGC CTCAGGCAAT GTCAGCTGGA GCCCCTGAAC 
CTCACCCGCC TCTGCGAGAC TCTGGAGAAG TGCCCCGGGC CTCTGGAAGT CGAATTGTCC 
TTTGAGGTCC TGAGCGACCA GAGCCTGGAG ACCCTGCTGC AGCACTTACC CCGGCTGCCC 
CAGCTAAGCC TGCTGCAGCT AAGCCAGACG AGGCTTTCCC CAAAGAGCCT CCTCCTGCTG 
GCCGACCTCT TCAGCTTATG CCCCCGGGTT CAGAAGGTGG ATATCAGGTC CCTGTATCAC 
ATGACACTGC ACTTCAGGTC TGGCGAGGAG CATGAAGGCG AGTGCTGTGG CAGGTTCACG 
GGCTGTGGCC TTAGTCAGGA GCATGTGGAG CCGCTCTGCT GGTTGCTGAG CAAATGCGAA 
GACCTCAACC AGTTGGACCT CTCAGCAAAC ATGCTGGGTG ATGACGGGCT CAGGTGCCTC 
CTGGAATGTC TGCCTCAATT GCCCATCTCT GGTTCGCTTG ACCTGAGTCA CAACAGCATC 
TCCCTAGAGA GTGCCCTGGG CCTGGTGAAG ACACTCCCCT CCTGCCCGCA CATCCGGGAG 
GCTTCTGTGA ACCTGGGCTC TGAGCAGAGC TTCTGGATCT ACTTCTCCCG AAAGGAGGAG 
GCCGGGAAGA CACTAAGGCT GAGCCAGTGC AACCTCATGC CAGAGCATGT GCCCAGACTG 
GCCACCCACC TGAACCAGGC CCTGAAGCTG ACAGCGCTCA CGCTGACCCA GTGCGGCCTG 
GGCCTGGAGC AGCTGATAGT TCTCCTGAGT CTGCTGAGAT GTCCAGTGGA CCTGTTCACT 
CTCAGGGTGG AGGAGCCGTG GGTAGGCAAA GCCGGGGTGC TCGCCCTGCT AAGAGTCTGC 
ACCCAGACCT CAGGCAACAT CACTGAAATA AGCATTTCGG AGACCCAGCA GCAGCTCTGT 
CTCCAGCTGG AGTTTCCCCC TCAGGAGAAC CCAGAAGCCG TGACGCTCAG GTTGGCTCAC 
TGTGACCTTG AGACCCACCA CAGCCTTCTT GCTGGGCAGC TGATGGAGAC ATGTGCCAGG 
CTGCAGCAGC TCAGCTTGTC CCAGGTGAAC CTCTGCGACT CCAGCTCCCT GCTGCTAAAA 
AACCTCCTGG TGTCTCTCTC TGAGCTGAAG ACATTCAGAC TGACCTCCAG CTGTATGAGC 
TCCAAGGGCC TGGCCCACCT GACATTTGGC CTGAGAAACT GCCACCACCT GGAGGAGCTG 
GACTTGTCCA ACAATCAGTT TGGTGAGGAG GACACTGAAG TCCTGATGAC GGTCCTTGAG 
GGCAAGTGCT GGCTGAAGAA GCTCGACCTC AGCCATCTCC CACTAGGCAG CTCCACCCTG 
GCCATGTTCA CTCAAGGACT AAGCCACATG ACCCTCCTAC AGAGCCTCCG GCTGAGCAGG 
AACGGTATTT GTGATGTCGG CTGCCACCAA CTTTCCGAGG CCCTCAGAGC TGCCATGAGC 
TTGAAGGAGC TGGGCTTGAG CCACAACCAG ATCAGAGACA CCGGTGCCCA GCACTTAGCT 
GCTGTCCTAC CAGGACTGCC GGAGCTCAGG AAGATAGACC TCTCAGGGAA TGGCATTGGT 
CCAGCTGGAG GGACGCGGCT GGCAGAGTCC CTCACCCTCT GCAGGCACTT GGAGGAGCTG 
ATGCTTGGCT ACAACGTCCT GGGGGATACC ACAGCCCTGG GCCTGGCCCA GGGACTGCCC 
TGCTGCCTGA GGGTCCTGCA CCTGCCGTCT AGCCGTCTGG GCCCAAAGGG AGCGCTGAGC 
CTGAGCCAGG CCCTGGATGG ATGCCCACAT GTGGAAGAGA TCAGCTTGGC AGAGAACAGT 
CTGGCCAGAG GGGTCCTGCA TTTCTGTCAG GGGCTCCCCC TGCTGAGGCA GATAGACCTG 
GTTTCGTGTG AGATCGACAA CCACACCGCC AAGCCCCTGG CTGCCAGCCT GGTGCTCTGC 
CCAGCCCTGG AAGAAATCCT GCTGTCCTGG AATCTGCTCG GGGACGAGGC GGCTGCCGAG 
TTGGCCCGGG TCCTGCCGCA GATGGGCCGG CTGAAGAGAA TGGACCTGGA GAAGAATCGG 
ATCACAGCTT GTGGAGCCTG GCTCCTGGCT GAAGGGCTGG CACAAGGGTC TGGCATCCAA 
GTCATTCGCC TCTGGAACAA TCCCATCTCC CCCACCATGG TCCAGCATCT ACAGAGCCAG 
GAGCCCAGGC TGGACTTCGC TTTCTTCGAC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964812.1
CDS	229..5724
Translation	MDHVSFQLGTTNLWSWLVRLLSKDPEWLNAKVKFFLPKMDLSSGDEAQDTTQRVMLQLRRLHTQGRATWQAFIHCVCMELDVPLDLEVPLLSTWGHGDGFPSQLGAGEESQPASQLHHGLKRPHQSCGSSPRLKQCKNHQLELARRYLKLLRTSAQQRYSRIPGPGQPLAFHQAYIPPILQWSRTTAPFSAQDGAVVGDPKAEDDTDVSIRDLFSTRADKGPRVTVLLGKAGMGKTTLAHRLCQRWAAGQLDRFQALFLFEFRQLNLITSSVTLPQLLFDLYLSPEAGPDSVFQYLEEHAGGVLLIFDGLDQALHSHSSTEPEGPEAPSPALALFSGLCRGTILPGCWVLATSRPGKLPDCLPTEAATVHMWGFDRPRVEEYVGRFFSDQEWQEAALAELRENRHLQSMCAVPALCQVTCLCLHHLLPGNSPGQSAALLPTVTQNYVQMVSTLSPRGYLPPESLLGLSEMALRGLETGKVIFSVGDISPPMVAFGAALGLLTSFCICTGPGHQGIGYAFTHLSLQEFFAALYLMASPKVDKNVLARHVTLNSRWVLRTKARLGLLDHLPTFLAGLASCACRPFLSHVAQQDEMWVSDKQAAVMLALRKLAIRRLTGPKVVALCHCVGETQEPELASLMAQSLPSHLSFHNFPLTYTDLSALTNILRHRNASIHLDFEGCPLEPHCPEALAGCKQIENLRLAGCKITARGISHLMQALRLCPQLEEISFQDNQLKDRAVLNIVEVLPSLPRLQKLDLSRSNVSVSTLLCLTKVAVTCPTVKMLQVRETDLIFLLSPPTETAAELGAAPDLQGNAIQRKEAQSRSLALRLLQCQLGIHDVQELIAQLREGPRLEEVNLSGNQLDDEGCRLMAEAASQLHITRNLDLSNNGLSVDRVHCMLSAVNTCQNLAELHISLLHKTVVFTFAPEQEEQEGIWKRTALRDSLTHQVPFELPLRSTRIRLTHCGLQAKHLEQLCRALGGSCHHSHLDLSGNALGDEGVAQLAQLLPGLGALQSLNLSENGLSLDTVFILIQCFSTLQWLLHLDIGFESQHVVLRGDRRGRDRLAGGCPPEFLAGVPFLAFDQCCVPESFCLRQCQLEPLNLTRLCETLEKCPGPLEVELSFEVLSDQSLETLLQHLPRLPQLSLLQLSQTRLSPKSLLLLADLFSLCPRVQKVDIRSLYHMTLHFRSGEEHEGECCGRFTGCGLSQEHVEPLCWLLSKCEDLNQLDLSANMLGDDGLRCLLECLPQLPISGSLDLSHNSISLESALGLVKTLPSCPHIREASVNLGSEQSFWIYFSRKEEAGKTLRLSQCNLMPEHVPRLATHLNQALKLTALTLTQCGLGLEQLIVLLSLLRCPVDLFTLRVEEPWVGKAGVLALLRVCTQTSGNITEISISETQQQLCLQLEFPPQENPEAVTLRLAHCDLETHHSLLAGQLMETCARLQQLSLSQVNLCDSSSLLLKNLLVSLSELKTFRLTSSCMSSKGLAHLTFGLRNCHHLEELDLSNNQFGEEDTEVLMTVLEGKCWLKKLDLSHLPLGSSTLAMFTQGLSHMTLLQSLRLSRNGICDVGCHQLSEALRAAMSLKELGLSHNQIRDTGAQHLAAVLPGLPELRKIDLSGNGIGPAGGTRLAESLTLCRHLEELMLGYNVLGDTTALGLAQGLPCCLRVLHLPSSRLGPKGALSLSQALDGCPHVEEISLAENSLARGVLHFCQGLPLLRQIDLVSCEIDNHTAKPLAASLVLCPALEEILLSWNLLGDEAAAELARVLPQMGRLKRMDLEKNRITACGAWLLAEGLAQGSGIQVIRLWNNPISPTMVQHLQSQEPRLDFAFFDK

Target ORF information:

RefSeq Version	XM_014109337.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NLR family CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109337.2

ORF Insert Sequence:

ATGGACCACG TTAGCTTCCA GCTGGGTACC ACTAACCTGT GGAGCTGGCT CGTGAGGCTG 
CTCAGTAAAG ACCCAGAATG GCTGAATGCC AAGGTGAAGT TCTTCCTCCC CAAGATGGAC 
CTGAGCTCCG GGGACGAGGC CCAGGACACC ACACAGAGGG TCATGCTACA GCTCAGAAGA 
TTGCACACCC AAGGTCGGGC CACTTGGCAG GCATTCATCC ACTGTGTGTG CATGGAGCTC 
GATGTGCCCC TGGACCTGGA GGTGCCACTG CTGAGCACTT GGGGCCATGG AGATGGGTTC 
CCCAGCCAGC TGGGGGCTGG TGAAGAAAGC CAACCTGCCT CTCAGCTCCA CCACGGTCTG 
AAGCGGCCTC ATCAGAGCTG TGGGTCCTCA CCCCGCCTGA AGCAGTGCAA GAATCATCAG 
CTCGAGTTGG CCAGGAGGTA CCTGAAGTTG CTGAGGACTT CTGCCCAGCA GCGCTACAGC 
AGGATTCCCG GGCCGGGGCA ACCCCTTGCC TTCCACCAGG CCTACATCCC CCCAATCCTG 
CAGTGGAGCC GGACCACGGC CCCCTTCAGT GCTCAGGACG GGGCTGTAGT GGGGGACCCC 
AAGGCAGAAG ATGACACGGA CGTGAGCATC CGGGACCTCT TCAGCACCAG GGCCGACAAG 
GGCCCGAGGG TGACGGTGCT CCTGGGGAAG GCAGGCATGG GCAAGACCAC GCTGGCCCAC 
CGGCTCTGCC AGAGGTGGGC TGCTGGCCAG CTTGACCGCT TCCAGGCTCT GTTCCTGTTT 
GAATTCCGTC AGCTCAATCT GATCACCAGT TCCGTGACGC TGCCCCAGCT GCTGTTTGAC 
CTGTACCTGA GCCCCGAGGC GGGCCCCGAC TCGGTCTTCC AGTACCTGGA GGAGCATGCT 
GGAGGAGTCC TGCTGATCTT TGATGGGCTG GACCAGGCCC TCCACTCGCA TTCCAGCACG 
GAGCCTGAAG GCCCCGAGGC CCCAAGTCCG GCCCTTGCCC TCTTCTCCGG GCTCTGCCGG 
GGGACCATCC TGCCTGGCTG CTGGGTTTTG GCCACCTCCC GTCCAGGGAA GCTGCCCGAC 
TGCCTGCCCA CAGAGGCAGC CACGGTCCAC ATGTGGGGCT TTGACAGGCC ACGGGTGGAG 
GAGTATGTGG GCCGCTTCTT CAGTGACCAA GAGTGGCAGG AAGCAGCCCT AGCAGAGCTG 
CGAGAAAACA GGCATCTCCA GAGCATGTGT GCGGTGCCTG CACTGTGCCA AGTCACCTGC 
CTCTGCCTCC ACCATCTGCT CCCAGGCAAC TCTCCAGGCC AGTCTGCAGC CCTCCTGCCC 
ACCGTGACTC AGAACTATGT ACAGATGGTG TCCACTCTCA GCCCCCGTGG GTACCTGCCT 
CCCGAGTCTC TGCTGGGCCT CAGTGAGATG GCTCTGAGGG GCCTGGAGAC TGGGAAGGTT 
ATCTTCTCTG TAGGCGACAT CTCTCCACCC ATGGTGGCTT TTGGGGCAGC CCTCGGCTTG 
TTGACCTCCT TCTGCATCTG CACAGGCCCT GGGCACCAGG GTATAGGCTA TGCCTTCACC 
CACCTCAGCC TACAAGAGTT TTTTGCCGCC TTGTACCTGA TGGCCAGCCC CAAGGTGGAC 
AAAAATGTAC TTGCCCGCCA TGTCACCCTC AATTCTCGCT GGGTGCTACG GACCAAAGCT 
AGACTAGGCC TCTTAGACCA CCTCCCCACC TTCCTGGCAG GGCTGGCATC CTGTGCCTGC 
CGACCCTTCC TTAGCCATGT GGCCCAGCAG GATGAGATGT GGGTGAGTGA CAAGCAGGCT 
GCAGTGATGC TGGCTTTGAG GAAGTTGGCC ATTCGCAGGC TCACAGGACC CAAGGTTGTA 
GCACTCTGTC ACTGTGTGGG TGAGACCCAG GAGCCTGAGC TGGCCAGCCT CATGGCCCAA 
AGTCTCCCCA GTCACCTGTC CTTCCACAAT TTCCCACTGA CCTATACGGA CCTGTCTGCC 
CTGACCAACA TCTTGAGGCA CAGGAATGCC TCCATCCACC TGGATTTTGA GGGCTGCCCC 
CTGGAGCCCC ACTGCCCCGA GGCCCTGGCA GGCTGTAAGC AGATAGAGAA TCTCAGGTTA 
GCAGGATGTA AGATCACTGC CCGAGGCATC AGCCACCTGA TGCAGGCTTT GCGCCTCTGT 
CCACAGCTGG AAGAGATCAG CTTCCAGGAC AACCAGCTGA AGGATAGGGC CGTGCTGAAT 
ATCGTGGAAG TACTTCCTTC TTTGCCACGG CTCCAGAAGC TTGACCTGAG CCGCAGCAAT 
GTCTCCGTGT CAACCTTACT CTGCTTGACA AAGGTGGCGG TCACATGCCC TACTGTCAAG 
ATGCTACAGG TCAGGGAGAC GGACCTCATC TTTCTTCTTT CCCCGCCCAC AGAGACTGCT 
GCAGAGCTTG GAGCAGCACC AGATCTACAG GGAAATGCCA TCCAGAGGAA AGAGGCTCAG 
AGCAGAAGCC TGGCACTCAG GCTCCTGCAG TGCCAGCTCG GAATTCATGA TGTGCAGGAG 
CTCATTGCCC AGCTCCGGGA AGGTCCCCGC CTGGAGGAAG TAAACCTTTC AGGAAACCAG 
CTGGATGATG AAGGCTGTCG ACTGATGGCA GAAGCTGCAT CCCAGCTGCA CATCACCAGG 
AATCTAGACC TCAGCAACAA CGGGCTATCT GTGGACAGGG TACACTGTAT GCTGAGTGCG 
GTGAACACAT GCCAGAACTT GGCAGAGCTT CATATCAGCC TGCTGCATAA AACTGTGGTC 
TTCACATTTG CCCCAGAGCA GGAGGAGCAG GAGGGGATCT GGAAGAGGAC TGCGCTTCGG 
GACAGCCTCA CGCACCAGGT GCCCTTTGAG CTGCCCCTGC GCTCCACGAG GATCAGGCTG 
ACACACTGTG GCTTGCAAGC CAAGCACCTA GAGCAGCTCT GCAGGGCCCT GGGAGGAAGC 
TGCCACCACA GTCACCTGGA CCTCTCAGGC AATGCTCTGG GGGATGAAGG CGTGGCCCAG 
CTGGCTCAGC TGCTCCCAGG GCTGGGTGCT CTGCAGTCCT TGAACCTCAG TGAGAATGGT 
TTGTCCCTGG ATACAGTGTT TATTTTGATC CAGTGCTTCT CGACTCTGCA GTGGCTTCTT 
CACTTGGATA TCGGCTTCGA GAGCCAGCAC GTCGTCCTGA GAGGTGACAG AAGAGGCAGG 
GATCGATTGG CTGGTGGATG TCCGCCAGAG TTCCTAGCTG GAGTCCCATT CTTGGCCTTT 
GATCAGTGCT GCGTCCCCGA GAGCTTCTGC CTCAGGCAAT GTCAGCTGGA GCCCCTGAAC 
CTCACCCGCC TCTGCGAGAC TCTGGAGAAG TGCCCCGGGC CTCTGGAAGT CGAATTGTCC 
TTTGAGGTCC TGAGCGACCA GAGCCTGGAG ACCCTGCTGC AGCACTTACC CCGGCTGCCC 
CAGCTAAGCC TGCTGCAGCT AAGCCAGACG AGGCTTTCCC CAAAGAGCCT CCTCCTGCTG 
GCCGACCTCT TCAGCTTATG CCCCCGGGTT CAGAAGGTGG ATATCAGGTC CCTGTATCAC 
ATGACACTGC ACTTCAGGTC TGGCGAGGAG CATGAAGGCG AGTGCTGTGG CAGGTTCACG 
GGCTGTGGCC TTAGTCAGGA GCATGTGGAG CCGCTCTGCT GGTTGCTGAG CAAATGCGAA 
GACCTCAACC AGTTGGACCT CTCAGCAAAC ATGCTGGGTG ATGACGGGCT CAGGTGCCTC 
CTGGAATGTC TGCCTCAATT GCCCATCTCT GGTTCGCTTG ACCTGAGTCA CAACAGCATC 
TCCCTAGAGA GTGCCCTGGG CCTGGTGAAG ACACTCCCCT CCTGCCCGCA CATCCGGGAG 
GCTTCTGTGA ACCTGGGCTC TGAGCAGAGC TTCTGGATCT ACTTCTCCCG AAAGGAGGAG 
GCCGGGAAGA CACTAAGGCT GAGCCAGTGC AACCTCATGC CAGAGCATGT GCCCAGACTG 
GCCACCCACC TGAACCAGGC CCTGAAGCTG ACAGCGCTCA CGCTGACCCA GTGCGGCCTG 
GGCCTGGAGC AGCTGATAGT TCTCCTGAGT CTGCTGAGAT GTCCAGTGGA CCTGTTCACT 
CTCAGGGTGG AGGAGCCGTG GGTAGGCAAA GCCGGGGTGC TCGCCCTGCT AAGAGTCTGC 
ACCCAGACCT CAGGCAACAT CACTGAAATA AGCATTTCGG AGACCCAGCA GCAGCTCTGT 
CTCCAGCTGG AGTTTCCCCC TCAGGAGAAC CCAGAAGCCG TGACGCTCAG GTTGGCTCAC 
TGTGACCTTG AGACCCACCA CAGCCTTCTT GCTGGGCAGC TGATGGAGAC ATGTGCCAGG 
CTGCAGCAGC TCAGCTTGTC CCAGGTGAAC CTCTGCGACT CCAGCTCCCT GCTGCTAAAA 
AACCTCCTGG TGTCTCTCTC TGAGCTGAAG ACATTCAGAC TGACCTCCAG CTGTATGAGC 
TCCAAGGGCC TGGCCCACCT GACATTTGGC CTGAGAAACT GCCACCACCT GGAGGAGCTG 
GACTTGTCCA ACAATCAGTT TGGTGAGGAG GACACTGAAG TCCTGATGAC GGTCCTTGAG 
GGCAAGTGCT GGCTGAAGAA GCTCGACCTC AGCCATCTCC CACTAGGCAG CTCCACCCTG 
GCCATGTTCA CTCAAGGACT AAGCCACATG ACCCTCCTAC AGAGCCTCCG GCTGAGCAGG 
AACGGTATTT GTGATGTCGG CTGCCACCAA CTTTCCGAGG CCCTCAGAGC TGCCATGAGC 
TTGAAGGAGC TGGGCTTGAG CCACAACCAG ATCAGAGACA CCGGTGCCCA GCACTTAGCT 
GCTGTCCTAC CAGGACTGCC GGAGCTCAGG AAGATAGACC TCTCAGGGAA TGGCATTGGT 
CCAGCTGGAG GGACGCGGCT GGCAGAGTCC CTCACCCTCT GCAGGCACTT GGAGGAGCTG 
ATGCTTGGCT ACAACGTCCT GGGGGATACC ACAGCCCTGG GCCTGGCCCA GGGACTGCCC 
TGCTGCCTGA GGGTCCTGCA CCTGCCGTCT AGCCGTCTGG GCCCAAAGGG AGCGCTGAGC 
CTGAGCCAGG CCCTGGATGG ATGCCCACAT GTGGAAGAGA TCAGCTTGGC AGAGAACAGT 
CTGGCCAGAG GGGTCCTGCA TTTCTGTCAG GGGCTCCCCC TGCTGAGGCA GATAGACCTG 
GTTTCGTGTG AGATCGACAA CCACACCGCC AAGCCCCTGG CTGCCAGCCT GGTGCTCTGC 
CCAGCCCTGG AAGAAATCCT GCTGTCCTGG AATCTGCTCG GGGACGAGGC GGCTGCCGAG 
TTGGCCCGGG TCCTGCCGCA GATGGGCCGG CTGAAGAGAA TGGACCTGGA GAAGAATCGG 
ATCACAGCTT GTGGAGCCTG GCTCCTGGCT GAAGGGCTGG CACAAGGGTC TGGCATCCAA 
GTCATTCGCC TCTGGAACAA TCCCATCTCC CCCACCATGG TCCAGCATCT ACAGAGCCAG 
GAGCCCAGGC TGGACTTCGC TTTCTTCGAC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NLRC5 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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