NEK1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEK1
Entrez Gene ID	486058
Full Name	NIMA related kinase 1
General protein information	Preferred Names NIMA related kinase 1 Names serine/threonine-protein kinase Nek1 NIMA (never in mitosis gene a)-related kinase 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014107564.2	XP_013963039.1	serine/threonine-protein kinase Nek1 isoform X1
XM_014107564.1	XP_013963039.1	serine/threonine-protein kinase Nek1 isoform X1
XM_014107565.1	XP_013963040.1	serine/threonine-protein kinase Nek1 isoform X1
XM_014107565.2	XP_013963040.1	serine/threonine-protein kinase Nek1 isoform X1
XM_022410111.1	XP_022265819.1	serine/threonine-protein kinase Nek1 isoform X3
XM_014107566.1	XP_013963041.1	serine/threonine-protein kinase Nek1 isoform X2
XM_022410110.1	XP_022265818.1	serine/threonine-protein kinase Nek1 isoform X1
XM_014107566.2	XP_013963041.1	serine/threonine-protein kinase Nek1 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NEK1	XP_003586402.2
Rattus norvegicus (Norway rat)	Nek1	NP_001099552.2
Gallus gallus (chicken)	NEK1	XP_420401.3
Homo sapiens (human)	NEK1	NP_001186326.1
	NEK1	XP_001083275.1
Canis lupus familiaris (dog)	NEK1	XP_543184.2
Mus musculus (house mouse)	Nek1	NP_780298.2
Pan troglodytes (chimpanzee)	NEK1	XP_526727.2
Danio rerio (zebrafish)	nek1	XP_005160601.1
Xenopus tropicalis (tropical clawed frog)	nek1	NP_001090680.1

Gene Ontology

The following NEK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe297348	XM_022410111.1 Latest version!	Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe43094	XM_022410110.1 Latest version!	Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe43094	XM_014107565.2 Latest version!	Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe43094	XM_014107564.2 Latest version!	Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe43095	XM_014107566.2 Latest version!	Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OCe43094	XM_014107564.1	Canis lupus familiaris NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe43094	XM_014107565.1	Canis lupus familiaris NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe43095	XM_014107566.1	Canis lupus familiaris NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEK1 ( XM_022410111.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265819.1 Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe297348
Clone ID Related Accession (Same CDS sequence)	XM_022410111.1
Accession Version	XM_022410111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAAGCTGAT 
GACTTAGAAA CAGAAGTTCT GCAAGAGCCA GGTGGAGCAA ACAAAGATGG TAGCTTGCCA 
TGCACGATAA TTGATGTGTG GATTAGTGAG ACAAAAGAAA CAAAGGAAAC TGAGTTTGAA 
GATAGGATCA CTGTTCAGCA AAATGAAGTT TCTGAAGATG AAATCCCAAT GAATGTGGAC 
CACCTTAGTG AAGTCGATAA AAGACCTGAA ATAGATGATT CTCAGCATTC TAAATGTGGT 
ATAGATAAAT CTGTGCAACC AGAGCCATTT TTCCATAGAG TGGTTCTTTC AAAACACTTG 
AATGCAGTTT CTCAAATTCA GTCAATTCTA TGTTCACCAG AAGAATCCTT TCCACTTCGA 
TCTCGCTCTG ATTCACCACC GAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTAT TTGATGCAAA CAACCCAAAG ATGCTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAATTGT TCAGAACACT GATGGATGTT CCTACTGTAG GAGATGTATG TCAAGACAAT 
CTTGAAATAG ATGATATTGA AGATGAGCAT ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTTTTTG AAGAAACTGA CACAGATTTA CAAGAGCTTC AGGCCTCTAT GGAACAGTTA 
CTTAGGGAAC AACCTGGAGA AGAATACAGT GAGGAGGAAG AATCAGTCTT AAAGAACAGT 
GACATAGAGC AGACTGCAAA TGGGACAGAT CTGGCAGATG AGGATGACAA TCCCAGCAGT 
GAAAGTGCCC TAAATGAAGA ATGGCACTCA GATAACAGTG ATGGTGATAT TACTAGTGAA 
TGTGAATATG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCACCT GGAACAGGAA 
ATAGGCTTTG AAAAATTCTT TGAGGTTTAC GAGAAAATAA AAGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAACAATA GTTCAGGATA TTTTAGGAAA TGAACATGAA 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTATCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265819.1
CDS	316..3864
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDDRQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRNLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKCGAQPIPAKRPASGQSLASVVSAQKITKPAAKYGVPLTYKKFGDKKLHEKKPLQKHKQAHQIPVKKVNPGEERRKMFEEAARKRRLEFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGCGEVKAPFFGSGGAVAPSSFSSRGQYEHYHAIFDQMQQQRVENNEAKWKGEIYGRRHPERQKGQLAVERAKQVEEFLQRKREARQNKARAEGHMGILQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEADYSEGQEGSEEADVRRKKVETFKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPPLGQHEAGGSPSKQQMRSVISVTSALKEVGMDRSLSDTQETSEDMQKANSAISSKREILRRLNQNLKAQEDEEEKQCHSDTCEIVVNEGAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSATEKHTMGEVIKLDPGGSPRKVWGRSPTDSVLKILGEAELQLQTELLENTAIRSEADDLETEVLQEPGGANKDGSLPCTIIDVWISETKETKETEFEDRITVQQNEVSEDEIPMNVDHLSEVDKRPEIDDSQHSKCGIDKSVQPEPFFHRVVLSKHLNAVSQIQSILCSPEESFPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVCQDNLEIDDIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGDITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAIHEDEDENIEICSTIVQDILGNEHEHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_022410111.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410111.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAAGCTGAT 
GACTTAGAAA CAGAAGTTCT GCAAGAGCCA GGTGGAGCAA ACAAAGATGG TAGCTTGCCA 
TGCACGATAA TTGATGTGTG GATTAGTGAG ACAAAAGAAA CAAAGGAAAC TGAGTTTGAA 
GATAGGATCA CTGTTCAGCA AAATGAAGTT TCTGAAGATG AAATCCCAAT GAATGTGGAC 
CACCTTAGTG AAGTCGATAA AAGACCTGAA ATAGATGATT CTCAGCATTC TAAATGTGGT 
ATAGATAAAT CTGTGCAACC AGAGCCATTT TTCCATAGAG TGGTTCTTTC AAAACACTTG 
AATGCAGTTT CTCAAATTCA GTCAATTCTA TGTTCACCAG AAGAATCCTT TCCACTTCGA 
TCTCGCTCTG ATTCACCACC GAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTAT TTGATGCAAA CAACCCAAAG ATGCTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAATTGT TCAGAACACT GATGGATGTT CCTACTGTAG GAGATGTATG TCAAGACAAT 
CTTGAAATAG ATGATATTGA AGATGAGCAT ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTTTTTG AAGAAACTGA CACAGATTTA CAAGAGCTTC AGGCCTCTAT GGAACAGTTA 
CTTAGGGAAC AACCTGGAGA AGAATACAGT GAGGAGGAAG AATCAGTCTT AAAGAACAGT 
GACATAGAGC AGACTGCAAA TGGGACAGAT CTGGCAGATG AGGATGACAA TCCCAGCAGT 
GAAAGTGCCC TAAATGAAGA ATGGCACTCA GATAACAGTG ATGGTGATAT TACTAGTGAA 
TGTGAATATG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCACCT GGAACAGGAA 
ATAGGCTTTG AAAAATTCTT TGAGGTTTAC GAGAAAATAA AAGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAACAATA GTTCAGGATA TTTTAGGAAA TGAACATGAA 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTATCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_014107564.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963039.1 Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe43094
Clone ID Related Accession (Same CDS sequence)	XM_014107564.2 , XM_014107564.1 , XM_014107565.1 , XM_014107565.2 , XM_022410110.1
Accession Version	XM_014107564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAGATTTCT 
CCTGAAAAGG AGAAGTATAA ATCTTTAATT ACTGGAGAAG AAAAAGTAAA ATATATCTCA 
CCTGAAATGG ATTCATCAGC TGCTGTTGAT TCTTCTGTTG AAACGAACAG TCCAGAGTTT 
AGAGAGTCAT CTCCTCAGAT ATCATTGAAA CCAGAAGGAA ATGTGGATGA AGCTGATGAC 
TTAGAAACAG AAGTTCTGCA AGAGCCAGGT GGAGCAAACA AAGATGGTAG CTTGCCATGC 
ACGATAATTG ATGTGTGGAT TAGTGAGACA AAAGAAACAA AGGAAACTGA GTTTGAAGAT 
AGGATCACTG TTCAGCAAAA TGAAGTTTCT GAAGATGAAA TCCCAATGAA TGTGGACCAC 
CTTAGTGAAG TCGATAAAAG ACCTGAAATA GATGATTCTC AGCATTCTAA ATGTGGTATA 
GATAAATCTG TGCAACCAGA GCCATTTTTC CATAGAGTGG TTCTTTCAAA ACACTTGAAT 
GCAGTTTCTC AAATTCAGTC AATTCTATGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CGCTCTGATT CACCACCGAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTATTTG ATGCAAACAA CCCAAAGATG CTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAATTGTTCA GAACACTGAT GGATGTTCCT ACTGTAGGAG ATGTATGTCA AGACAATCTT 
GAAATAGATG ATATTGAAGA TGAGCATATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTTTTTGAAG AAACTGACAC AGATTTACAA GAGCTTCAGG CCTCTATGGA ACAGTTACTT 
AGGGAACAAC CTGGAGAAGA ATACAGTGAG GAGGAAGAAT CAGTCTTAAA GAACAGTGAC 
ATAGAGCAGA CTGCAAATGG GACAGATCTG GCAGATGAGG ATGACAATCC CAGCAGTGAA 
AGTGCCCTAA ATGAAGAATG GCACTCAGAT AACAGTGATG GTGATATTAC TAGTGAATGT 
GAATATGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCACCTGGA ACAGGAAATA 
GGCTTTGAAA AATTCTTTGA GGTTTACGAG AAAATAAAAG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AACAATAGTT CAGGATATTT TAGGAAATGA ACATGAACAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTATCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963039.1
CDS	316..4041
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDDRQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRNLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKCGAQPIPAKRPASGQSLASVVSAQKITKPAAKYGVPLTYKKFGDKKLHEKKPLQKHKQAHQIPVKKVNPGEERRKMFEEAARKRRLEFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGCGEVKAPFFGSGGAVAPSSFSSRGQYEHYHAIFDQMQQQRVENNEAKWKGEIYGRRHPERQKGQLAVERAKQVEEFLQRKREARQNKARAEGHMGILQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEADYSEGQEGSEEADVRRKKVETFKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPPLGQHEAGGSPSKQQMRSVISVTSALKEVGMDRSLSDTQETSEDMQKANSAISSKREILRRLNQNLKAQEDEEEKQCHSDTCEIVVNEGAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSATEKHTMGEVIKLDPGGSPRKVWGRSPTDSVLKILGEAELQLQTELLENTAIRSEISPEKEKYKSLITGEEKVKYISPEMDSSAAVDSSVETNSPEFRESSPQISLKPEGNVDEADDLETEVLQEPGGANKDGSLPCTIIDVWISETKETKETEFEDRITVQQNEVSEDEIPMNVDHLSEVDKRPEIDDSQHSKCGIDKSVQPEPFFHRVVLSKHLNAVSQIQSILCSPEESFPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVCQDNLEIDDIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGDITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAIHEDEDENIEICSTIVQDILGNEHEHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_014107564.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107564.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAGATTTCT 
CCTGAAAAGG AGAAGTATAA ATCTTTAATT ACTGGAGAAG AAAAAGTAAA ATATATCTCA 
CCTGAAATGG ATTCATCAGC TGCTGTTGAT TCTTCTGTTG AAACGAACAG TCCAGAGTTT 
AGAGAGTCAT CTCCTCAGAT ATCATTGAAA CCAGAAGGAA ATGTGGATGA AGCTGATGAC 
TTAGAAACAG AAGTTCTGCA AGAGCCAGGT GGAGCAAACA AAGATGGTAG CTTGCCATGC 
ACGATAATTG ATGTGTGGAT TAGTGAGACA AAAGAAACAA AGGAAACTGA GTTTGAAGAT 
AGGATCACTG TTCAGCAAAA TGAAGTTTCT GAAGATGAAA TCCCAATGAA TGTGGACCAC 
CTTAGTGAAG TCGATAAAAG ACCTGAAATA GATGATTCTC AGCATTCTAA ATGTGGTATA 
GATAAATCTG TGCAACCAGA GCCATTTTTC CATAGAGTGG TTCTTTCAAA ACACTTGAAT 
GCAGTTTCTC AAATTCAGTC AATTCTATGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CGCTCTGATT CACCACCGAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTATTTG ATGCAAACAA CCCAAAGATG CTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAATTGTTCA GAACACTGAT GGATGTTCCT ACTGTAGGAG ATGTATGTCA AGACAATCTT 
GAAATAGATG ATATTGAAGA TGAGCATATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTTTTTGAAG AAACTGACAC AGATTTACAA GAGCTTCAGG CCTCTATGGA ACAGTTACTT 
AGGGAACAAC CTGGAGAAGA ATACAGTGAG GAGGAAGAAT CAGTCTTAAA GAACAGTGAC 
ATAGAGCAGA CTGCAAATGG GACAGATCTG GCAGATGAGG ATGACAATCC CAGCAGTGAA 
AGTGCCCTAA ATGAAGAATG GCACTCAGAT AACAGTGATG GTGATATTAC TAGTGAATGT 
GAATATGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCACCTGGA ACAGGAAATA 
GGCTTTGAAA AATTCTTTGA GGTTTACGAG AAAATAAAAG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AACAATAGTT CAGGATATTT TAGGAAATGA ACATGAACAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTATCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_014107564.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963039.1 Canis lupus familiaris NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.

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CloneID	OCe43094
Clone ID Related Accession (Same CDS sequence)	XM_014107564.2 , XM_014107564.1 , XM_014107565.1 , XM_014107565.2 , XM_022410110.1
Accession Version	XM_014107564.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAGATTTCT 
CCTGAAAAGG AGAAGTATAA ATCTTTAATT ACTGGAGAAG AAAAAGTAAA ATATATCTCA 
CCTGAAATGG ATTCATCAGC TGCTGTTGAT TCTTCTGTTG AAACGAACAG TCCAGAGTTT 
AGAGAGTCAT CTCCTCAGAT ATCATTGAAA CCAGAAGGAA ATGTGGATGA AGCTGATGAC 
TTAGAAACAG AAGTTCTGCA AGAGCCAGGT GGAGCAAACA AAGATGGTAG CTTGCCATGC 
ACGATAATTG ATGTGTGGAT TAGTGAGACA AAAGAAACAA AGGAAACTGA GTTTGAAGAT 
AGGATCACTG TTCAGCAAAA TGAAGTTTCT GAAGATGAAA TCCCAATGAA TGTGGACCAC 
CTTAGTGAAG TCGATAAAAG ACCTGAAATA GATGATTCTC AGCATTCTAA ATGTGGTATA 
GATAAATCTG TGCAACCAGA GCCATTTTTC CATAGAGTGG TTCTTTCAAA ACACTTGAAT 
GCAGTTTCTC AAATTCAGTC AATTCTATGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CGCTCTGATT CACCACCGAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTATTTG ATGCAAACAA CCCAAAGATG CTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAATTGTTCA GAACACTGAT GGATGTTCCT ACTGTAGGAG ATGTATGTCA AGACAATCTT 
GAAATAGATG ATATTGAAGA TGAGCATATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTTTTTGAAG AAACTGACAC AGATTTACAA GAGCTTCAGG CCTCTATGGA ACAGTTACTT 
AGGGAACAAC CTGGAGAAGA ATACAGTGAG GAGGAAGAAT CAGTCTTAAA GAACAGTGAC 
ATAGAGCAGA CTGCAAATGG GACAGATCTG GCAGATGAGG ATGACAATCC CAGCAGTGAA 
AGTGCCCTAA ATGAAGAATG GCACTCAGAT AACAGTGATG GTGATATTAC TAGTGAATGT 
GAATATGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCACCTGGA ACAGGAAATA 
GGCTTTGAAA AATTCTTTGA GGTTTACGAG AAAATAAAAG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AACAATAGTT CAGGATATTT TAGGAAATGA ACATGAACAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTATCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963039.1
CDS	303..4028
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDDRQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRNLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKCGAQPIPAKRPASGQSLASVVSAQKITKPAAKYGVPLTYKKFGDKKLHEKKPLQKHKQAHQIPVKKVNPGEERRKMFEEAARKRRLEFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGCGEVKAPFFGSGGAVAPSSFSSRGQYEHYHAIFDQMQQQRVENNEAKWKGEIYGRRHPERQKGQLAVERAKQVEEFLQRKREARQNKARAEGHMGILQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEADYSEGQEGSEEADVRRKKVETFKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPPLGQHEAGGSPSKQQMRSVISVTSALKEVGMDRSLSDTQETSEDMQKANSAISSKREILRRLNQNLKAQEDEEEKQCHSDTCEIVVNEGAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSATEKHTMGEVIKLDPGGSPRKVWGRSPTDSVLKILGEAELQLQTELLENTAIRSEISPEKEKYKSLITGEEKVKYISPEMDSSAAVDSSVETNSPEFRESSPQISLKPEGNVDEADDLETEVLQEPGGANKDGSLPCTIIDVWISETKETKETEFEDRITVQQNEVSEDEIPMNVDHLSEVDKRPEIDDSQHSKCGIDKSVQPEPFFHRVVLSKHLNAVSQIQSILCSPEESFPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVCQDNLEIDDIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGDITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAIHEDEDENIEICSTIVQDILGNEHEHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_014107564.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107564.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAGATTTCT 
CCTGAAAAGG AGAAGTATAA ATCTTTAATT ACTGGAGAAG AAAAAGTAAA ATATATCTCA 
CCTGAAATGG ATTCATCAGC TGCTGTTGAT TCTTCTGTTG AAACGAACAG TCCAGAGTTT 
AGAGAGTCAT CTCCTCAGAT ATCATTGAAA CCAGAAGGAA ATGTGGATGA AGCTGATGAC 
TTAGAAACAG AAGTTCTGCA AGAGCCAGGT GGAGCAAACA AAGATGGTAG CTTGCCATGC 
ACGATAATTG ATGTGTGGAT TAGTGAGACA AAAGAAACAA AGGAAACTGA GTTTGAAGAT 
AGGATCACTG TTCAGCAAAA TGAAGTTTCT GAAGATGAAA TCCCAATGAA TGTGGACCAC 
CTTAGTGAAG TCGATAAAAG ACCTGAAATA GATGATTCTC AGCATTCTAA ATGTGGTATA 
GATAAATCTG TGCAACCAGA GCCATTTTTC CATAGAGTGG TTCTTTCAAA ACACTTGAAT 
GCAGTTTCTC AAATTCAGTC AATTCTATGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CGCTCTGATT CACCACCGAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTATTTG ATGCAAACAA CCCAAAGATG CTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAATTGTTCA GAACACTGAT GGATGTTCCT ACTGTAGGAG ATGTATGTCA AGACAATCTT 
GAAATAGATG ATATTGAAGA TGAGCATATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTTTTTGAAG AAACTGACAC AGATTTACAA GAGCTTCAGG CCTCTATGGA ACAGTTACTT 
AGGGAACAAC CTGGAGAAGA ATACAGTGAG GAGGAAGAAT CAGTCTTAAA GAACAGTGAC 
ATAGAGCAGA CTGCAAATGG GACAGATCTG GCAGATGAGG ATGACAATCC CAGCAGTGAA 
AGTGCCCTAA ATGAAGAATG GCACTCAGAT AACAGTGATG GTGATATTAC TAGTGAATGT 
GAATATGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCACCTGGA ACAGGAAATA 
GGCTTTGAAA AATTCTTTGA GGTTTACGAG AAAATAAAAG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AACAATAGTT CAGGATATTT TAGGAAATGA ACATGAACAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTATCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_014107565.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963040.1 Canis lupus familiaris NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe43094
Clone ID Related Accession (Same CDS sequence)	XM_014107564.2 , XM_014107564.1 , XM_014107565.1 , XM_014107565.2 , XM_022410110.1
Accession Version	XM_014107565.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAGATTTCT 
CCTGAAAAGG AGAAGTATAA ATCTTTAATT ACTGGAGAAG AAAAAGTAAA ATATATCTCA 
CCTGAAATGG ATTCATCAGC TGCTGTTGAT TCTTCTGTTG AAACGAACAG TCCAGAGTTT 
AGAGAGTCAT CTCCTCAGAT ATCATTGAAA CCAGAAGGAA ATGTGGATGA AGCTGATGAC 
TTAGAAACAG AAGTTCTGCA AGAGCCAGGT GGAGCAAACA AAGATGGTAG CTTGCCATGC 
ACGATAATTG ATGTGTGGAT TAGTGAGACA AAAGAAACAA AGGAAACTGA GTTTGAAGAT 
AGGATCACTG TTCAGCAAAA TGAAGTTTCT GAAGATGAAA TCCCAATGAA TGTGGACCAC 
CTTAGTGAAG TCGATAAAAG ACCTGAAATA GATGATTCTC AGCATTCTAA ATGTGGTATA 
GATAAATCTG TGCAACCAGA GCCATTTTTC CATAGAGTGG TTCTTTCAAA ACACTTGAAT 
GCAGTTTCTC AAATTCAGTC AATTCTATGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CGCTCTGATT CACCACCGAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTATTTG ATGCAAACAA CCCAAAGATG CTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAATTGTTCA GAACACTGAT GGATGTTCCT ACTGTAGGAG ATGTATGTCA AGACAATCTT 
GAAATAGATG ATATTGAAGA TGAGCATATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTTTTTGAAG AAACTGACAC AGATTTACAA GAGCTTCAGG CCTCTATGGA ACAGTTACTT 
AGGGAACAAC CTGGAGAAGA ATACAGTGAG GAGGAAGAAT CAGTCTTAAA GAACAGTGAC 
ATAGAGCAGA CTGCAAATGG GACAGATCTG GCAGATGAGG ATGACAATCC CAGCAGTGAA 
AGTGCCCTAA ATGAAGAATG GCACTCAGAT AACAGTGATG GTGATATTAC TAGTGAATGT 
GAATATGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCACCTGGA ACAGGAAATA 
GGCTTTGAAA AATTCTTTGA GGTTTACGAG AAAATAAAAG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AACAATAGTT CAGGATATTT TAGGAAATGA ACATGAACAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTATCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963040.1
CDS	752..4477
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDDRQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRNLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKCGAQPIPAKRPASGQSLASVVSAQKITKPAAKYGVPLTYKKFGDKKLHEKKPLQKHKQAHQIPVKKVNPGEERRKMFEEAARKRRLEFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGCGEVKAPFFGSGGAVAPSSFSSRGQYEHYHAIFDQMQQQRVENNEAKWKGEIYGRRHPERQKGQLAVERAKQVEEFLQRKREARQNKARAEGHMGILQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEADYSEGQEGSEEADVRRKKVETFKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPPLGQHEAGGSPSKQQMRSVISVTSALKEVGMDRSLSDTQETSEDMQKANSAISSKREILRRLNQNLKAQEDEEEKQCHSDTCEIVVNEGAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSATEKHTMGEVIKLDPGGSPRKVWGRSPTDSVLKILGEAELQLQTELLENTAIRSEISPEKEKYKSLITGEEKVKYISPEMDSSAAVDSSVETNSPEFRESSPQISLKPEGNVDEADDLETEVLQEPGGANKDGSLPCTIIDVWISETKETKETEFEDRITVQQNEVSEDEIPMNVDHLSEVDKRPEIDDSQHSKCGIDKSVQPEPFFHRVVLSKHLNAVSQIQSILCSPEESFPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVCQDNLEIDDIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGDITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAIHEDEDENIEICSTIVQDILGNEHEHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_014107565.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107565.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAGATTTCT 
CCTGAAAAGG AGAAGTATAA ATCTTTAATT ACTGGAGAAG AAAAAGTAAA ATATATCTCA 
CCTGAAATGG ATTCATCAGC TGCTGTTGAT TCTTCTGTTG AAACGAACAG TCCAGAGTTT 
AGAGAGTCAT CTCCTCAGAT ATCATTGAAA CCAGAAGGAA ATGTGGATGA AGCTGATGAC 
TTAGAAACAG AAGTTCTGCA AGAGCCAGGT GGAGCAAACA AAGATGGTAG CTTGCCATGC 
ACGATAATTG ATGTGTGGAT TAGTGAGACA AAAGAAACAA AGGAAACTGA GTTTGAAGAT 
AGGATCACTG TTCAGCAAAA TGAAGTTTCT GAAGATGAAA TCCCAATGAA TGTGGACCAC 
CTTAGTGAAG TCGATAAAAG ACCTGAAATA GATGATTCTC AGCATTCTAA ATGTGGTATA 
GATAAATCTG TGCAACCAGA GCCATTTTTC CATAGAGTGG TTCTTTCAAA ACACTTGAAT 
GCAGTTTCTC AAATTCAGTC AATTCTATGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CGCTCTGATT CACCACCGAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTATTTG ATGCAAACAA CCCAAAGATG CTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAATTGTTCA GAACACTGAT GGATGTTCCT ACTGTAGGAG ATGTATGTCA AGACAATCTT 
GAAATAGATG ATATTGAAGA TGAGCATATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTTTTTGAAG AAACTGACAC AGATTTACAA GAGCTTCAGG CCTCTATGGA ACAGTTACTT 
AGGGAACAAC CTGGAGAAGA ATACAGTGAG GAGGAAGAAT CAGTCTTAAA GAACAGTGAC 
ATAGAGCAGA CTGCAAATGG GACAGATCTG GCAGATGAGG ATGACAATCC CAGCAGTGAA 
AGTGCCCTAA ATGAAGAATG GCACTCAGAT AACAGTGATG GTGATATTAC TAGTGAATGT 
GAATATGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCACCTGGA ACAGGAAATA 
GGCTTTGAAA AATTCTTTGA GGTTTACGAG AAAATAAAAG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AACAATAGTT CAGGATATTT TAGGAAATGA ACATGAACAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTATCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_014107565.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963040.1 Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe43094
Clone ID Related Accession (Same CDS sequence)	XM_014107564.2 , XM_014107564.1 , XM_014107565.1 , XM_014107565.2 , XM_022410110.1
Accession Version	XM_014107565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAGATTTCT 
CCTGAAAAGG AGAAGTATAA ATCTTTAATT ACTGGAGAAG AAAAAGTAAA ATATATCTCA 
CCTGAAATGG ATTCATCAGC TGCTGTTGAT TCTTCTGTTG AAACGAACAG TCCAGAGTTT 
AGAGAGTCAT CTCCTCAGAT ATCATTGAAA CCAGAAGGAA ATGTGGATGA AGCTGATGAC 
TTAGAAACAG AAGTTCTGCA AGAGCCAGGT GGAGCAAACA AAGATGGTAG CTTGCCATGC 
ACGATAATTG ATGTGTGGAT TAGTGAGACA AAAGAAACAA AGGAAACTGA GTTTGAAGAT 
AGGATCACTG TTCAGCAAAA TGAAGTTTCT GAAGATGAAA TCCCAATGAA TGTGGACCAC 
CTTAGTGAAG TCGATAAAAG ACCTGAAATA GATGATTCTC AGCATTCTAA ATGTGGTATA 
GATAAATCTG TGCAACCAGA GCCATTTTTC CATAGAGTGG TTCTTTCAAA ACACTTGAAT 
GCAGTTTCTC AAATTCAGTC AATTCTATGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CGCTCTGATT CACCACCGAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTATTTG ATGCAAACAA CCCAAAGATG CTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAATTGTTCA GAACACTGAT GGATGTTCCT ACTGTAGGAG ATGTATGTCA AGACAATCTT 
GAAATAGATG ATATTGAAGA TGAGCATATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTTTTTGAAG AAACTGACAC AGATTTACAA GAGCTTCAGG CCTCTATGGA ACAGTTACTT 
AGGGAACAAC CTGGAGAAGA ATACAGTGAG GAGGAAGAAT CAGTCTTAAA GAACAGTGAC 
ATAGAGCAGA CTGCAAATGG GACAGATCTG GCAGATGAGG ATGACAATCC CAGCAGTGAA 
AGTGCCCTAA ATGAAGAATG GCACTCAGAT AACAGTGATG GTGATATTAC TAGTGAATGT 
GAATATGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCACCTGGA ACAGGAAATA 
GGCTTTGAAA AATTCTTTGA GGTTTACGAG AAAATAAAAG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AACAATAGTT CAGGATATTT TAGGAAATGA ACATGAACAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTATCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963040.1
CDS	708..4433
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDDRQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRNLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKCGAQPIPAKRPASGQSLASVVSAQKITKPAAKYGVPLTYKKFGDKKLHEKKPLQKHKQAHQIPVKKVNPGEERRKMFEEAARKRRLEFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGCGEVKAPFFGSGGAVAPSSFSSRGQYEHYHAIFDQMQQQRVENNEAKWKGEIYGRRHPERQKGQLAVERAKQVEEFLQRKREARQNKARAEGHMGILQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEADYSEGQEGSEEADVRRKKVETFKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPPLGQHEAGGSPSKQQMRSVISVTSALKEVGMDRSLSDTQETSEDMQKANSAISSKREILRRLNQNLKAQEDEEEKQCHSDTCEIVVNEGAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSATEKHTMGEVIKLDPGGSPRKVWGRSPTDSVLKILGEAELQLQTELLENTAIRSEISPEKEKYKSLITGEEKVKYISPEMDSSAAVDSSVETNSPEFRESSPQISLKPEGNVDEADDLETEVLQEPGGANKDGSLPCTIIDVWISETKETKETEFEDRITVQQNEVSEDEIPMNVDHLSEVDKRPEIDDSQHSKCGIDKSVQPEPFFHRVVLSKHLNAVSQIQSILCSPEESFPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVCQDNLEIDDIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGDITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAIHEDEDENIEICSTIVQDILGNEHEHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_014107565.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107565.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAGATTTCT 
CCTGAAAAGG AGAAGTATAA ATCTTTAATT ACTGGAGAAG AAAAAGTAAA ATATATCTCA 
CCTGAAATGG ATTCATCAGC TGCTGTTGAT TCTTCTGTTG AAACGAACAG TCCAGAGTTT 
AGAGAGTCAT CTCCTCAGAT ATCATTGAAA CCAGAAGGAA ATGTGGATGA AGCTGATGAC 
TTAGAAACAG AAGTTCTGCA AGAGCCAGGT GGAGCAAACA AAGATGGTAG CTTGCCATGC 
ACGATAATTG ATGTGTGGAT TAGTGAGACA AAAGAAACAA AGGAAACTGA GTTTGAAGAT 
AGGATCACTG TTCAGCAAAA TGAAGTTTCT GAAGATGAAA TCCCAATGAA TGTGGACCAC 
CTTAGTGAAG TCGATAAAAG ACCTGAAATA GATGATTCTC AGCATTCTAA ATGTGGTATA 
GATAAATCTG TGCAACCAGA GCCATTTTTC CATAGAGTGG TTCTTTCAAA ACACTTGAAT 
GCAGTTTCTC AAATTCAGTC AATTCTATGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CGCTCTGATT CACCACCGAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTATTTG ATGCAAACAA CCCAAAGATG CTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAATTGTTCA GAACACTGAT GGATGTTCCT ACTGTAGGAG ATGTATGTCA AGACAATCTT 
GAAATAGATG ATATTGAAGA TGAGCATATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTTTTTGAAG AAACTGACAC AGATTTACAA GAGCTTCAGG CCTCTATGGA ACAGTTACTT 
AGGGAACAAC CTGGAGAAGA ATACAGTGAG GAGGAAGAAT CAGTCTTAAA GAACAGTGAC 
ATAGAGCAGA CTGCAAATGG GACAGATCTG GCAGATGAGG ATGACAATCC CAGCAGTGAA 
AGTGCCCTAA ATGAAGAATG GCACTCAGAT AACAGTGATG GTGATATTAC TAGTGAATGT 
GAATATGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCACCTGGA ACAGGAAATA 
GGCTTTGAAA AATTCTTTGA GGTTTACGAG AAAATAAAAG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AACAATAGTT CAGGATATTT TAGGAAATGA ACATGAACAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTATCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_022410110.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265818.1 Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

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CloneID	OCe43094
Clone ID Related Accession (Same CDS sequence)	XM_014107564.2 , XM_014107564.1 , XM_014107565.1 , XM_014107565.2 , XM_022410110.1
Accession Version	XM_022410110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAGATTTCT 
CCTGAAAAGG AGAAGTATAA ATCTTTAATT ACTGGAGAAG AAAAAGTAAA ATATATCTCA 
CCTGAAATGG ATTCATCAGC TGCTGTTGAT TCTTCTGTTG AAACGAACAG TCCAGAGTTT 
AGAGAGTCAT CTCCTCAGAT ATCATTGAAA CCAGAAGGAA ATGTGGATGA AGCTGATGAC 
TTAGAAACAG AAGTTCTGCA AGAGCCAGGT GGAGCAAACA AAGATGGTAG CTTGCCATGC 
ACGATAATTG ATGTGTGGAT TAGTGAGACA AAAGAAACAA AGGAAACTGA GTTTGAAGAT 
AGGATCACTG TTCAGCAAAA TGAAGTTTCT GAAGATGAAA TCCCAATGAA TGTGGACCAC 
CTTAGTGAAG TCGATAAAAG ACCTGAAATA GATGATTCTC AGCATTCTAA ATGTGGTATA 
GATAAATCTG TGCAACCAGA GCCATTTTTC CATAGAGTGG TTCTTTCAAA ACACTTGAAT 
GCAGTTTCTC AAATTCAGTC AATTCTATGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CGCTCTGATT CACCACCGAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTATTTG ATGCAAACAA CCCAAAGATG CTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAATTGTTCA GAACACTGAT GGATGTTCCT ACTGTAGGAG ATGTATGTCA AGACAATCTT 
GAAATAGATG ATATTGAAGA TGAGCATATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTTTTTGAAG AAACTGACAC AGATTTACAA GAGCTTCAGG CCTCTATGGA ACAGTTACTT 
AGGGAACAAC CTGGAGAAGA ATACAGTGAG GAGGAAGAAT CAGTCTTAAA GAACAGTGAC 
ATAGAGCAGA CTGCAAATGG GACAGATCTG GCAGATGAGG ATGACAATCC CAGCAGTGAA 
AGTGCCCTAA ATGAAGAATG GCACTCAGAT AACAGTGATG GTGATATTAC TAGTGAATGT 
GAATATGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCACCTGGA ACAGGAAATA 
GGCTTTGAAA AATTCTTTGA GGTTTACGAG AAAATAAAAG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AACAATAGTT CAGGATATTT TAGGAAATGA ACATGAACAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTATCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265818.1
CDS	176..3901
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDDRQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRNLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKCGAQPIPAKRPASGQSLASVVSAQKITKPAAKYGVPLTYKKFGDKKLHEKKPLQKHKQAHQIPVKKVNPGEERRKMFEEAARKRRLEFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGCGEVKAPFFGSGGAVAPSSFSSRGQYEHYHAIFDQMQQQRVENNEAKWKGEIYGRRHPERQKGQLAVERAKQVEEFLQRKREARQNKARAEGHMGILQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEADYSEGQEGSEEADVRRKKVETFKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPPLGQHEAGGSPSKQQMRSVISVTSALKEVGMDRSLSDTQETSEDMQKANSAISSKREILRRLNQNLKAQEDEEEKQCHSDTCEIVVNEGAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSATEKHTMGEVIKLDPGGSPRKVWGRSPTDSVLKILGEAELQLQTELLENTAIRSEISPEKEKYKSLITGEEKVKYISPEMDSSAAVDSSVETNSPEFRESSPQISLKPEGNVDEADDLETEVLQEPGGANKDGSLPCTIIDVWISETKETKETEFEDRITVQQNEVSEDEIPMNVDHLSEVDKRPEIDDSQHSKCGIDKSVQPEPFFHRVVLSKHLNAVSQIQSILCSPEESFPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVCQDNLEIDDIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGDITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAIHEDEDENIEICSTIVQDILGNEHEHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_022410110.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410110.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GGAATCCTGC AAAACCTGGC AGCTATGTAT 
GGAGGCAGGT CCAGCTCTTC AAGAGGAGGG AAGCCAAGAA ACAAAGAGGA AGAGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTGCAG AATTTCAATG AACGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTGAT TATTCTGAGG GACAAGAAGG AAGTGAGGAG 
GCTGACGTGC GGCGTAAAAA AGTTGAAACA TTTAAGGCCC AAGCAAGTGC ACGAGCTTCT 
GTACTAAAAG AACAATTAGA ACGAAAGCGA AAGGAAGCAT ATGAGAGAGA AAAGAAAGTA 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTA AAGAATTCTG ATATGTCTCC ACCTTTGGGA 
CAACATGAAG CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTGACT 
TCAGCTTTGA AAGAAGTGGG TATGGATAGA AGTTTAAGTG ATACCCAGGA AACCTCAGAA 
GACATGCAGA AGGCTAACAG TGCTATTTCA AGTAAGCGGG AAATACTACG TAGGTTAAAT 
CAAAATCTTA AAGCTCAAGA AGATGAGGAA GAAAAGCAAT GTCACTCTGA TACATGTGAG 
ATAGTTGTTA ATGAAGGTGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAATGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTGAC ATTGGACACA 
TCCTTTTCTG CAACTGAAAA ACATACAATG GGAGAGGTTA TTAAATTAGA TCCTGGTGGA 
TCTCCAAGAA AAGTCTGGGG GAGAAGTCCT ACAGATTCAG TTCTAAAGAT ACTTGGGGAA 
GCTGAACTGC AACTTCAGAC AGAACTATTG GAAAACACAG CCATTAGAAG TGAGATTTCT 
CCTGAAAAGG AGAAGTATAA ATCTTTAATT ACTGGAGAAG AAAAAGTAAA ATATATCTCA 
CCTGAAATGG ATTCATCAGC TGCTGTTGAT TCTTCTGTTG AAACGAACAG TCCAGAGTTT 
AGAGAGTCAT CTCCTCAGAT ATCATTGAAA CCAGAAGGAA ATGTGGATGA AGCTGATGAC 
TTAGAAACAG AAGTTCTGCA AGAGCCAGGT GGAGCAAACA AAGATGGTAG CTTGCCATGC 
ACGATAATTG ATGTGTGGAT TAGTGAGACA AAAGAAACAA AGGAAACTGA GTTTGAAGAT 
AGGATCACTG TTCAGCAAAA TGAAGTTTCT GAAGATGAAA TCCCAATGAA TGTGGACCAC 
CTTAGTGAAG TCGATAAAAG ACCTGAAATA GATGATTCTC AGCATTCTAA ATGTGGTATA 
GATAAATCTG TGCAACCAGA GCCATTTTTC CATAGAGTGG TTCTTTCAAA ACACTTGAAT 
GCAGTTTCTC AAATTCAGTC AATTCTATGT TCACCAGAAG AATCCTTTCC ACTTCGATCT 
CGCTCTGATT CACCACCGAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTATTTG ATGCAAACAA CCCAAAGATG CTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAATTGTTCA GAACACTGAT GGATGTTCCT ACTGTAGGAG ATGTATGTCA AGACAATCTT 
GAAATAGATG ATATTGAAGA TGAGCATATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTTTTTGAAG AAACTGACAC AGATTTACAA GAGCTTCAGG CCTCTATGGA ACAGTTACTT 
AGGGAACAAC CTGGAGAAGA ATACAGTGAG GAGGAAGAAT CAGTCTTAAA GAACAGTGAC 
ATAGAGCAGA CTGCAAATGG GACAGATCTG GCAGATGAGG ATGACAATCC CAGCAGTGAA 
AGTGCCCTAA ATGAAGAATG GCACTCAGAT AACAGTGATG GTGATATTAC TAGTGAATGT 
GAATATGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCACCTGGA ACAGGAAATA 
GGCTTTGAAA AATTCTTTGA GGTTTACGAG AAAATAAAAG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AACAATAGTT CAGGATATTT TAGGAAATGA ACATGAACAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTATCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_014107566.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963041.1 Canis lupus familiaris NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe43095
Clone ID Related Accession (Same CDS sequence)	XM_014107566.1 , XM_014107566.2
Accession Version	XM_014107566.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GTTTATCTGG CAAGACTGAG GCAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AAAGCCAAAC TTCGTGGTGA AAAGAAAGAA 
GCTGATTATT CTGAGGGACA AGAAGGAAGT GAGGAGGCTG ACGTGCGGCG TAAAAAAGTT 
GAAACATTTA AGGCCCAAGC AAGTGCACGA GCTTCTGTAC TAAAAGAACA ATTAGAACGA 
AAGCGAAAGG AAGCATATGA GAGAGAAAAG AAAGTATGGG AAGAGCATTT GGTGGCTAAA 
GGAGTAAAGA ATTCTGATAT GTCTCCACCT TTGGGACAAC ATGAAGCAGG TGGCTCTCCA 
TCAAAGCAAC AGATGAGATC TGTTATTTCT GTGACTTCAG CTTTGAAAGA AGTGGGTATG 
GATAGAAGTT TAAGTGATAC CCAGGAAACC TCAGAAGACA TGCAGAAGGC TAACAGTGCT 
ATTTCAAGTA AGCGGGAAAT ACTACGTAGG TTAAATCAAA ATCTTAAAGC TCAAGAAGAT 
GAGGAAGAAA AGCAATGTCA CTCTGATACA TGTGAGATAG TTGTTAATGA AGGTGCCAAA 
GAGCATGAAA AAGAAAAATC AGTTTCATCT GATCGCAAGA AATGGGAGGC AGGAGGTCAA 
CTTGTGATTC CTCTGGATGA GTTGACATTG GACACATCCT TTTCTGCAAC TGAAAAACAT 
ACAATGGGAG AGGTTATTAA ATTAGATCCT GGTGGATCTC CAAGAAAAGT CTGGGGGAGA 
AGTCCTACAG ATTCAGTTCT AAAGATACTT GGGGAAGCTG AACTGCAACT TCAGACAGAA 
CTATTGGAAA ACACAGCCAT TAGAAGTGAG ATTTCTCCTG AAAAGGAGAA GTATAAATCT 
TTAATTACTG GAGAAGAAAA AGTAAAATAT ATCTCACCTG AAATGGATTC ATCAGCTGCT 
GTTGATTCTT CTGTTGAAAC GAACAGTCCA GAGTTTAGAG AGTCATCTCC TCAGATATCA 
TTGAAACCAG AAGGAAATGT GGATGAAGCT GATGACTTAG AAACAGAAGT TCTGCAAGAG 
CCAGGTGGAG CAAACAAAGA TGGTAGCTTG CCATGCACGA TAATTGATGT GTGGATTAGT 
GAGACAAAAG AAACAAAGGA AACTGAGTTT GAAGATAGGA TCACTGTTCA GCAAAATGAA 
GTTTCTGAAG ATGAAATCCC AATGAATGTG GACCACCTTA GTGAAGTCGA TAAAAGACCT 
GAAATAGATG ATTCTCAGCA TTCTAAATGT GGTATAGATA AATCTGTGCA ACCAGAGCCA 
TTTTTCCATA GAGTGGTTCT TTCAAAACAC TTGAATGCAG TTTCTCAAAT TCAGTCAATT 
CTATGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCGCT CTGATTCACC ACCGAAAAAT 
AAAAACAAGA ATTCCTTGCT GATTGGACTT TCAACTGGTC TATTTGATGC AAACAACCCA 
AAGATGCTAA GGACATGTTC ACTTCCAGAT CTCTCAAAAT TGTTCAGAAC ACTGATGGAT 
GTTCCTACTG TAGGAGATGT ATGTCAAGAC AATCTTGAAA TAGATGATAT TGAAGATGAG 
CATATTAAAG AAGGACCTTC TGATTCTGAA GACATTGTTT TTGAAGAAAC TGACACAGAT 
TTACAAGAGC TTCAGGCCTC TATGGAACAG TTACTTAGGG AACAACCTGG AGAAGAATAC 
AGTGAGGAGG AAGAATCAGT CTTAAAGAAC AGTGACATAG AGCAGACTGC AAATGGGACA 
GATCTGGCAG ATGAGGATGA CAATCCCAGC AGTGAAAGTG CCCTAAATGA AGAATGGCAC 
TCAGATAACA GTGATGGTGA TATTACTAGT GAATGTGAAT ATGATAGTGT CTTTAACCAT 
TTAGAGGAAC TGAGACTTCA CCTGGAACAG GAAATAGGCT TTGAAAAATT CTTTGAGGTT 
TACGAGAAAA TAAAAGCTAT TCATGAAGAT GAAGATGAAA ATATTGAAAT TTGTTCAACA 
ATAGTTCAGG ATATTTTAGG AAATGAACAT GAACATCTTT ATGCCAAGAT TCTTCATTTA 
GTCATGGCAG ATGGAGCCTA TCAAGAAGAT AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963041.1
CDS	303..3944
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDDRQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRNLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKCGAQPIPAKRPASGQSLASVVSAQKITKPAAKYGVPLTYKKFGDKKLHEKKPLQKHKQAHQIPVKKVNPGEERRKMFEEAARKRRLEFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGCGEVKAPFFGSGGAVAPSSFSSRGQYEHYHAIFDQMQQQRVENNEAKWKGEIYGRRHPERQKGQLAVERAKQVEEFLQRKREARQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEADYSEGQEGSEEADVRRKKVETFKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPPLGQHEAGGSPSKQQMRSVISVTSALKEVGMDRSLSDTQETSEDMQKANSAISSKREILRRLNQNLKAQEDEEEKQCHSDTCEIVVNEGAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSATEKHTMGEVIKLDPGGSPRKVWGRSPTDSVLKILGEAELQLQTELLENTAIRSEISPEKEKYKSLITGEEKVKYISPEMDSSAAVDSSVETNSPEFRESSPQISLKPEGNVDEADDLETEVLQEPGGANKDGSLPCTIIDVWISETKETKETEFEDRITVQQNEVSEDEIPMNVDHLSEVDKRPEIDDSQHSKCGIDKSVQPEPFFHRVVLSKHLNAVSQIQSILCSPEESFPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVCQDNLEIDDIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGDITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAIHEDEDENIEICSTIVQDILGNEHEHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_014107566.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107566.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GTTTATCTGG CAAGACTGAG GCAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AAAGCCAAAC TTCGTGGTGA AAAGAAAGAA 
GCTGATTATT CTGAGGGACA AGAAGGAAGT GAGGAGGCTG ACGTGCGGCG TAAAAAAGTT 
GAAACATTTA AGGCCCAAGC AAGTGCACGA GCTTCTGTAC TAAAAGAACA ATTAGAACGA 
AAGCGAAAGG AAGCATATGA GAGAGAAAAG AAAGTATGGG AAGAGCATTT GGTGGCTAAA 
GGAGTAAAGA ATTCTGATAT GTCTCCACCT TTGGGACAAC ATGAAGCAGG TGGCTCTCCA 
TCAAAGCAAC AGATGAGATC TGTTATTTCT GTGACTTCAG CTTTGAAAGA AGTGGGTATG 
GATAGAAGTT TAAGTGATAC CCAGGAAACC TCAGAAGACA TGCAGAAGGC TAACAGTGCT 
ATTTCAAGTA AGCGGGAAAT ACTACGTAGG TTAAATCAAA ATCTTAAAGC TCAAGAAGAT 
GAGGAAGAAA AGCAATGTCA CTCTGATACA TGTGAGATAG TTGTTAATGA AGGTGCCAAA 
GAGCATGAAA AAGAAAAATC AGTTTCATCT GATCGCAAGA AATGGGAGGC AGGAGGTCAA 
CTTGTGATTC CTCTGGATGA GTTGACATTG GACACATCCT TTTCTGCAAC TGAAAAACAT 
ACAATGGGAG AGGTTATTAA ATTAGATCCT GGTGGATCTC CAAGAAAAGT CTGGGGGAGA 
AGTCCTACAG ATTCAGTTCT AAAGATACTT GGGGAAGCTG AACTGCAACT TCAGACAGAA 
CTATTGGAAA ACACAGCCAT TAGAAGTGAG ATTTCTCCTG AAAAGGAGAA GTATAAATCT 
TTAATTACTG GAGAAGAAAA AGTAAAATAT ATCTCACCTG AAATGGATTC ATCAGCTGCT 
GTTGATTCTT CTGTTGAAAC GAACAGTCCA GAGTTTAGAG AGTCATCTCC TCAGATATCA 
TTGAAACCAG AAGGAAATGT GGATGAAGCT GATGACTTAG AAACAGAAGT TCTGCAAGAG 
CCAGGTGGAG CAAACAAAGA TGGTAGCTTG CCATGCACGA TAATTGATGT GTGGATTAGT 
GAGACAAAAG AAACAAAGGA AACTGAGTTT GAAGATAGGA TCACTGTTCA GCAAAATGAA 
GTTTCTGAAG ATGAAATCCC AATGAATGTG GACCACCTTA GTGAAGTCGA TAAAAGACCT 
GAAATAGATG ATTCTCAGCA TTCTAAATGT GGTATAGATA AATCTGTGCA ACCAGAGCCA 
TTTTTCCATA GAGTGGTTCT TTCAAAACAC TTGAATGCAG TTTCTCAAAT TCAGTCAATT 
CTATGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCGCT CTGATTCACC ACCGAAAAAT 
AAAAACAAGA ATTCCTTGCT GATTGGACTT TCAACTGGTC TATTTGATGC AAACAACCCA 
AAGATGCTAA GGACATGTTC ACTTCCAGAT CTCTCAAAAT TGTTCAGAAC ACTGATGGAT 
GTTCCTACTG TAGGAGATGT ATGTCAAGAC AATCTTGAAA TAGATGATAT TGAAGATGAG 
CATATTAAAG AAGGACCTTC TGATTCTGAA GACATTGTTT TTGAAGAAAC TGACACAGAT 
TTACAAGAGC TTCAGGCCTC TATGGAACAG TTACTTAGGG AACAACCTGG AGAAGAATAC 
AGTGAGGAGG AAGAATCAGT CTTAAAGAAC AGTGACATAG AGCAGACTGC AAATGGGACA 
GATCTGGCAG ATGAGGATGA CAATCCCAGC AGTGAAAGTG CCCTAAATGA AGAATGGCAC 
TCAGATAACA GTGATGGTGA TATTACTAGT GAATGTGAAT ATGATAGTGT CTTTAACCAT 
TTAGAGGAAC TGAGACTTCA CCTGGAACAG GAAATAGGCT TTGAAAAATT CTTTGAGGTT 
TACGAGAAAA TAAAAGCTAT TCATGAAGAT GAAGATGAAA ATATTGAAAT TTGTTCAACA 
ATAGTTCAGG ATATTTTAGG AAATGAACAT GAACATCTTT ATGCCAAGAT TCTTCATTTA 
GTCATGGCAG ATGGAGCCTA TCAAGAAGAT AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_014107566.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963041.1 Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe43095
Clone ID Related Accession (Same CDS sequence)	XM_014107566.1 , XM_014107566.2
Accession Version	XM_014107566.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GTTTATCTGG CAAGACTGAG GCAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AAAGCCAAAC TTCGTGGTGA AAAGAAAGAA 
GCTGATTATT CTGAGGGACA AGAAGGAAGT GAGGAGGCTG ACGTGCGGCG TAAAAAAGTT 
GAAACATTTA AGGCCCAAGC AAGTGCACGA GCTTCTGTAC TAAAAGAACA ATTAGAACGA 
AAGCGAAAGG AAGCATATGA GAGAGAAAAG AAAGTATGGG AAGAGCATTT GGTGGCTAAA 
GGAGTAAAGA ATTCTGATAT GTCTCCACCT TTGGGACAAC ATGAAGCAGG TGGCTCTCCA 
TCAAAGCAAC AGATGAGATC TGTTATTTCT GTGACTTCAG CTTTGAAAGA AGTGGGTATG 
GATAGAAGTT TAAGTGATAC CCAGGAAACC TCAGAAGACA TGCAGAAGGC TAACAGTGCT 
ATTTCAAGTA AGCGGGAAAT ACTACGTAGG TTAAATCAAA ATCTTAAAGC TCAAGAAGAT 
GAGGAAGAAA AGCAATGTCA CTCTGATACA TGTGAGATAG TTGTTAATGA AGGTGCCAAA 
GAGCATGAAA AAGAAAAATC AGTTTCATCT GATCGCAAGA AATGGGAGGC AGGAGGTCAA 
CTTGTGATTC CTCTGGATGA GTTGACATTG GACACATCCT TTTCTGCAAC TGAAAAACAT 
ACAATGGGAG AGGTTATTAA ATTAGATCCT GGTGGATCTC CAAGAAAAGT CTGGGGGAGA 
AGTCCTACAG ATTCAGTTCT AAAGATACTT GGGGAAGCTG AACTGCAACT TCAGACAGAA 
CTATTGGAAA ACACAGCCAT TAGAAGTGAG ATTTCTCCTG AAAAGGAGAA GTATAAATCT 
TTAATTACTG GAGAAGAAAA AGTAAAATAT ATCTCACCTG AAATGGATTC ATCAGCTGCT 
GTTGATTCTT CTGTTGAAAC GAACAGTCCA GAGTTTAGAG AGTCATCTCC TCAGATATCA 
TTGAAACCAG AAGGAAATGT GGATGAAGCT GATGACTTAG AAACAGAAGT TCTGCAAGAG 
CCAGGTGGAG CAAACAAAGA TGGTAGCTTG CCATGCACGA TAATTGATGT GTGGATTAGT 
GAGACAAAAG AAACAAAGGA AACTGAGTTT GAAGATAGGA TCACTGTTCA GCAAAATGAA 
GTTTCTGAAG ATGAAATCCC AATGAATGTG GACCACCTTA GTGAAGTCGA TAAAAGACCT 
GAAATAGATG ATTCTCAGCA TTCTAAATGT GGTATAGATA AATCTGTGCA ACCAGAGCCA 
TTTTTCCATA GAGTGGTTCT TTCAAAACAC TTGAATGCAG TTTCTCAAAT TCAGTCAATT 
CTATGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCGCT CTGATTCACC ACCGAAAAAT 
AAAAACAAGA ATTCCTTGCT GATTGGACTT TCAACTGGTC TATTTGATGC AAACAACCCA 
AAGATGCTAA GGACATGTTC ACTTCCAGAT CTCTCAAAAT TGTTCAGAAC ACTGATGGAT 
GTTCCTACTG TAGGAGATGT ATGTCAAGAC AATCTTGAAA TAGATGATAT TGAAGATGAG 
CATATTAAAG AAGGACCTTC TGATTCTGAA GACATTGTTT TTGAAGAAAC TGACACAGAT 
TTACAAGAGC TTCAGGCCTC TATGGAACAG TTACTTAGGG AACAACCTGG AGAAGAATAC 
AGTGAGGAGG AAGAATCAGT CTTAAAGAAC AGTGACATAG AGCAGACTGC AAATGGGACA 
GATCTGGCAG ATGAGGATGA CAATCCCAGC AGTGAAAGTG CCCTAAATGA AGAATGGCAC 
TCAGATAACA GTGATGGTGA TATTACTAGT GAATGTGAAT ATGATAGTGT CTTTAACCAT 
TTAGAGGAAC TGAGACTTCA CCTGGAACAG GAAATAGGCT TTGAAAAATT CTTTGAGGTT 
TACGAGAAAA TAAAAGCTAT TCATGAAGAT GAAGATGAAA ATATTGAAAT TTGTTCAACA 
ATAGTTCAGG ATATTTTAGG AAATGAACAT GAACATCTTT ATGCCAAGAT TCTTCATTTA 
GTCATGGCAG ATGGAGCCTA TCAAGAAGAT AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963041.1
CDS	316..3957
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDDRQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRNLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKCGAQPIPAKRPASGQSLASVVSAQKITKPAAKYGVPLTYKKFGDKKLHEKKPLQKHKQAHQIPVKKVNPGEERRKMFEEAARKRRLEFIEKEKKQKDQISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGCGEVKAPFFGSGGAVAPSSFSSRGQYEHYHAIFDQMQQQRVENNEAKWKGEIYGRRHPERQKGQLAVERAKQVEEFLQRKREARQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEADYSEGQEGSEEADVRRKKVETFKAQASARASVLKEQLERKRKEAYEREKKVWEEHLVAKGVKNSDMSPPLGQHEAGGSPSKQQMRSVISVTSALKEVGMDRSLSDTQETSEDMQKANSAISSKREILRRLNQNLKAQEDEEEKQCHSDTCEIVVNEGAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSATEKHTMGEVIKLDPGGSPRKVWGRSPTDSVLKILGEAELQLQTELLENTAIRSEISPEKEKYKSLITGEEKVKYISPEMDSSAAVDSSVETNSPEFRESSPQISLKPEGNVDEADDLETEVLQEPGGANKDGSLPCTIIDVWISETKETKETEFEDRITVQQNEVSEDEIPMNVDHLSEVDKRPEIDDSQHSKCGIDKSVQPEPFFHRVVLSKHLNAVSQIQSILCSPEESFPLRSRSDSPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVCQDNLEIDDIEDEHIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDIEQTANGTDLADEDDNPSSESALNEEWHSDNSDGDITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAIHEDEDENIEICSTIVQDILGNEHEHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_014107566.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107566.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGA CAGACAATAT GTTATCAAAG AAATTAACAT CTCAAGAATG 
TCCAATAAAG AAAGGGAGGA ATCAAGAAGA GAAGTTGCAG TGTTGGCAAA CATGAAGCAT 
CCAAATATTG TTCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATCGTGATG 
GATTACTGTG AAGGAGGTGA TCTGTTTAAA CGAATTAATG CTCAGAAAGG CATTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCTTGAA ACATGTACAT 
GATCGAAAAA TTCTTCATCG AGACATAAAG TCACAGAATA TATTTTTAAC AAAAGATGGG 
ACAATACAAC TGGGAGATTT TGGAATCGCT AGAGTACTTA ATAGTACTGT GGAGCTGGCT 
AGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TTTGTGAAAA CAAACCTTAC 
AACAATAAAA GTGATATTTG GGCTCTGGGG TGTGTCCTAT ATGAGATGTG CACACTTAAA 
CATGCATTCG AAGCTGGCAA TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTAT CTTTACATTA TTCTTATGAT CTCCGCAATT TACTCTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC ACCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCCAAGTGTG GAGCGCAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAGCTTGGCT 
TCTGTTGTGT CTGCTCAGAA AATTACAAAG CCTGCTGCTA AATATGGAGT ACCTTTAACA 
TATAAGAAAT TTGGAGATAA AAAATTGCAT GAAAAGAAAC CACTTCAAAA ACATAAACAG 
GCCCATCAAA TTCCAGTGAA GAAAGTGAAT CCTGGAGAAG AAAGGAGGAA AATGTTTGAG 
GAAGCAGCAA GAAAGAGAAG GTTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTAGTTTAA TGAAGGCGGA GCAGATGAAA AGACAGGAAA AGGAAAGGTT GGAGAGAATA 
AATAGGGCCA GGGAACAAGG ATGGAGAAAC GTGCTAAGTG CTGGTGGATG TGGTGAAGTA 
AAGGCTCCCT TTTTTGGCAG TGGAGGGGCA GTGGCTCCAT CATCTTTTTC TTCTCGAGGA 
CAATATGAAC ATTACCATGC TATTTTTGAT CAAATGCAGC AACAAAGAGT AGAAAATAAT 
GAAGCGAAAT GGAAAGGAGA AATATATGGC CGAAGACATC CAGAAAGGCA AAAGGGGCAA 
CTAGCTGTGG AAAGAGCTAA ACAAGTGGAA GAGTTCCTAC AGCGAAAACG GGAAGCACGG 
CAAAATAAAG CTCGAGCCGA AGGACATATG GTTTATCTGG CAAGACTGAG GCAAATAAGA 
CTGCAGAATT TCAATGAACG CCAACAGATT AAAGCCAAAC TTCGTGGTGA AAAGAAAGAA 
GCTGATTATT CTGAGGGACA AGAAGGAAGT GAGGAGGCTG ACGTGCGGCG TAAAAAAGTT 
GAAACATTTA AGGCCCAAGC AAGTGCACGA GCTTCTGTAC TAAAAGAACA ATTAGAACGA 
AAGCGAAAGG AAGCATATGA GAGAGAAAAG AAAGTATGGG AAGAGCATTT GGTGGCTAAA 
GGAGTAAAGA ATTCTGATAT GTCTCCACCT TTGGGACAAC ATGAAGCAGG TGGCTCTCCA 
TCAAAGCAAC AGATGAGATC TGTTATTTCT GTGACTTCAG CTTTGAAAGA AGTGGGTATG 
GATAGAAGTT TAAGTGATAC CCAGGAAACC TCAGAAGACA TGCAGAAGGC TAACAGTGCT 
ATTTCAAGTA AGCGGGAAAT ACTACGTAGG TTAAATCAAA ATCTTAAAGC TCAAGAAGAT 
GAGGAAGAAA AGCAATGTCA CTCTGATACA TGTGAGATAG TTGTTAATGA AGGTGCCAAA 
GAGCATGAAA AAGAAAAATC AGTTTCATCT GATCGCAAGA AATGGGAGGC AGGAGGTCAA 
CTTGTGATTC CTCTGGATGA GTTGACATTG GACACATCCT TTTCTGCAAC TGAAAAACAT 
ACAATGGGAG AGGTTATTAA ATTAGATCCT GGTGGATCTC CAAGAAAAGT CTGGGGGAGA 
AGTCCTACAG ATTCAGTTCT AAAGATACTT GGGGAAGCTG AACTGCAACT TCAGACAGAA 
CTATTGGAAA ACACAGCCAT TAGAAGTGAG ATTTCTCCTG AAAAGGAGAA GTATAAATCT 
TTAATTACTG GAGAAGAAAA AGTAAAATAT ATCTCACCTG AAATGGATTC ATCAGCTGCT 
GTTGATTCTT CTGTTGAAAC GAACAGTCCA GAGTTTAGAG AGTCATCTCC TCAGATATCA 
TTGAAACCAG AAGGAAATGT GGATGAAGCT GATGACTTAG AAACAGAAGT TCTGCAAGAG 
CCAGGTGGAG CAAACAAAGA TGGTAGCTTG CCATGCACGA TAATTGATGT GTGGATTAGT 
GAGACAAAAG AAACAAAGGA AACTGAGTTT GAAGATAGGA TCACTGTTCA GCAAAATGAA 
GTTTCTGAAG ATGAAATCCC AATGAATGTG GACCACCTTA GTGAAGTCGA TAAAAGACCT 
GAAATAGATG ATTCTCAGCA TTCTAAATGT GGTATAGATA AATCTGTGCA ACCAGAGCCA 
TTTTTCCATA GAGTGGTTCT TTCAAAACAC TTGAATGCAG TTTCTCAAAT TCAGTCAATT 
CTATGTTCAC CAGAAGAATC CTTTCCACTT CGATCTCGCT CTGATTCACC ACCGAAAAAT 
AAAAACAAGA ATTCCTTGCT GATTGGACTT TCAACTGGTC TATTTGATGC AAACAACCCA 
AAGATGCTAA GGACATGTTC ACTTCCAGAT CTCTCAAAAT TGTTCAGAAC ACTGATGGAT 
GTTCCTACTG TAGGAGATGT ATGTCAAGAC AATCTTGAAA TAGATGATAT TGAAGATGAG 
CATATTAAAG AAGGACCTTC TGATTCTGAA GACATTGTTT TTGAAGAAAC TGACACAGAT 
TTACAAGAGC TTCAGGCCTC TATGGAACAG TTACTTAGGG AACAACCTGG AGAAGAATAC 
AGTGAGGAGG AAGAATCAGT CTTAAAGAAC AGTGACATAG AGCAGACTGC AAATGGGACA 
GATCTGGCAG ATGAGGATGA CAATCCCAGC AGTGAAAGTG CCCTAAATGA AGAATGGCAC 
TCAGATAACA GTGATGGTGA TATTACTAGT GAATGTGAAT ATGATAGTGT CTTTAACCAT 
TTAGAGGAAC TGAGACTTCA CCTGGAACAG GAAATAGGCT TTGAAAAATT CTTTGAGGTT 
TACGAGAAAA TAAAAGCTAT TCATGAAGAT GAAGATGAAA ATATTGAAAT TTGTTCAACA 
ATAGTTCAGG ATATTTTAGG AAATGAACAT GAACATCTTT ATGCCAAGAT TCTTCATTTA 
GTCATGGCAG ATGGAGCCTA TCAAGAAGAT AATGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NEK1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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