DIAPH3 cDNA ORF clone, Canis lupus familiaris(dog)

The following DIAPH3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIAPH3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe23596 XM_005633946.2
Latest version!
Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe23596 XM_005633947.3
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe23596 XM_005633948.3
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe23597 XM_022408011.1
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe23597 XM_005633950.2
Latest version!
Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe23597 XM_005633951.3
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe23598 XM_005633952.3
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe23592 XM_005633942.2
Latest version!
Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe23589 XM_542593.6
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe23590 XM_005633940.3
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe23593 XM_005633943.1
Latest version!
Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe23595 XM_005633945.3
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe296160 XM_022408007.1
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe296161 XM_022408008.1
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe296162 XM_022408009.1
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe296163 XM_022408010.1
Latest version!
Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OCe23596 XM_005633948.2 Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe23589 XM_542593.5 Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe23595 XM_005633945.2 Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe23596 XM_005633947.2 Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe23597 XM_005633951.2 Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe23590 XM_005633940.2 Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe23598 XM_005633952.2 Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe23596
    Clone ID Related Accession (Same CDS sequence) XM_005633947.2 , XM_005633948.3 , XM_005633948.2 , XM_005633947.3 , XM_005633946.2
    Accession Version XM_005633947.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein diaphanous homolog 3 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTAACGG CTGAACATTT CCACCTCAGG CAGGGGACAA GTTGGGTAGA AAGCTTTGGA 
    CGCGAGGGGC TTGGATTATT ACTGGACATT TTGGAAAGAC TGATTAGTGG CAAAATACAA
    GAGAAAATTG TAAAGAAGAA TCAGCATAAA GTCATACAGT GTCTAAAGGC TTTGATGAAT
    ACACAGTATG GCTTGGAAAG AATTATGAGT GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA
    GCCATCGATC CTGAGCACCC AAGTATGATG ACAGATGTGG TGAAACTCCT CTCTGCGGTA
    TGCATTGTAG GAGAGGAAAG CATTCTCGAA GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA
    GAAGAAAGAA AAATCGACAG ATTTTCATCC ATTGTGGAAG GTCTCCGGCA TGATTCAGTT
    CAACTGCAAG TAGCGTGTAT GCAGCTCATC AATGCCCTTG TTACATCTCC TGATGATTTG
    GACTTCAGGC TTCACATCAG AAATGAATTT ATGCGTTGTG GATTGAAAGA GATCTTGCCA
    GATTTAAAAT GTATGAAGAA TGATAGCCTG GATATCCAAC TTAAAGTCTT TGATGAGCAT
    AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT CGCCTTGAAG ATATTAGAGC TGAACTTGAT
    GAAGCATATG ATATTTACAA CATGGTGTGG AACTCAGTTA AGGAAACTAA AGCAGAGGGA
    TATTTCATTT CTATTCTTCA GCACCTTTTG CTGATTCGAA ATGATTATAG TATAAGGCAA
    CAGTACTTCA AATTAATTGA TGAGTGTATA TCTCAGATTG TATTGCATAG AGATGGGATG
    GATCCAGACT TCACATATCG GAAAAGACTG GATTTAGATT TAAGTCAGTT TGTTGATACT
    TGCATAGATC AAGCAAAACT AGAAGAGTTT GAAGAGAAAG CGTCAGAACT GCACAAGAAA
    TTTGAAAAAG AGTTTACCGA CCACCAAGAA ACTCAGGCTC AATTGCAGAA AAAAGAAGCA
    AAGATTAATG AACTTCAAGC AGAGTTACAA GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA
    GCTGGTAGAA ATCACTCTTT GCCACAGTCA AAAGAAAATG GAACTGGCCC CCCAGCACTT
    GCTCCTCCCC TGCCACTGCC ATCTTGTGGA GGCATGCCGC CTCCACCCCC TCCTCCCCCA
    CCTCCTCCGC TTCCAGGAAT GCCAATGCCT TTTGGTGGTC CCGTGCCTCC TCCACCACCT
    CCTCTGGGAT TTCTCAGTGG GCGAAACTCT CCTCCTCCAC CAACCCTACC ATTTGGGTTA
    AAACCAAAGA AAGAATTTAA ACCTGAAACC AGCATGAGAA GATTGAATTG GTTAAAGATC
    AGACCTCACG AAATGACTGA AAGCTGTTTC TGGATCAAAG TAAATGAAAA TAAGTATGAA
    AATGTGGATT TGCTCTGTAA ACTTGAGAAT ACGTTTTGTT GCCAACAAAA AGAGAGAAGA
    GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT ATTAAGAAAA AAATTAAAGA GCTTAAATTT
    TTAGATTCTA AAATTGCCCA GAATCTTTCC ATCTTCCTGA GCTCTTTTCG GGTGCCATAT
    GAGGAAATCA AAACGATGAT ATTGGAAGTG GATGAAACAC AGTTGGCAGA GTCTATGATT
    CAGAACCTAA TAAAGCATCT TCCTGATCAA GAACAATTAA GCTCACTGTC TCAGTTCAAG
    AATGACTATA ACAACCTATG TGAACCCGAG CAGTTTGCTG TTGTGATGAG CAATGTGAAG
    AGACTACGGC CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC
    AACATCAAAC CTGACATCAT GGCTGTCAGT ACTGCCTGCG AGGAGATAAA GAAGAGCAAA
    AGCTTCAGCA AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC
    CGGAATGCTC AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACGAAA
    TCAGCAGATC AGAAAACAAC ACTACTTCAT TTTCTGGTGG AAGTATGTGA AGAAAAGTAT
    CCTGATATCC TGAATTTTGT GGATGATTTG GAACATTTAG ACAAAGCTAG TAAAGTCTCT
    GTAGAAACGC TGGAAAAGAA TTTGAAGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAA
    GATTTGGAAA CCTTTCCCCC TCCTGAGGAC TTGCATGATA AGTTTGTGAC AAAGATGTCC
    AGCTTTGTTA TCAGCGCAAA AGAACAATAT GAGAAACTTC TAAAGTTACA TGAAAATATG
    GAGAAGTTAT ACCAGAGTGT GATGGGATAC TATGCCATTG ACGTGAAGAA GATGTCCGTG
    GAAGACTTTT TTAATGACTT GAATAATTTC AGAACCACAT TCATGCAAGC AAGAAAGGAG
    AACATCAAAA AAAGAGAAGC AGAGGAAAGG GAAAAACGTG CCCAAATAGC CAAAGAATTG
    GCAGAGAAAG AAAGACTTGA ACGCCAACAA AAGAAAAAGC GCTTGTTAGA AATGAAGACT
    GAGGGTGATG AGACAGGAGT GATGGATAGT CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC
    TTCCGCGACC GAAGAAAAAG GACGCCAAAG CCAAAAGATA TTCGGCAAAG TCTCAGTCCC
    ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT AACCATGAAA ATCAGAAAGT GCAGTTGACA
    GAAGGGTCAC GTTCACACTA CAATATCAAT TGCAACTCAA CGAGGACTCC AGTCGCCAAG
    GAGCTTAATT ATAATCTAGA CACTCATACG TCTACAGGGA GGATCAAGGC AGTTGAGAAG
    AAGGAAGCCT GTAATGTAGA AAGCAACAGA AAAAAGGAAA TGGAACTTCT TGGCTCGGTT
    TCTAAAAACG AATCTGTTCC CGAAGTTGAA GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634004.1
    CDS267..3266
    Translation

    Target ORF information:

    RefSeq Version XM_005633947.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633947.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTAACGG CTGAACATTT CCACCTCAGG CAGGGGACAA GTTGGGTAGA AAGCTTTGGA 
    CGCGAGGGGC TTGGATTATT ACTGGACATT TTGGAAAGAC TGATTAGTGG CAAAATACAA
    GAGAAAATTG TAAAGAAGAA TCAGCATAAA GTCATACAGT GTCTAAAGGC TTTGATGAAT
    ACACAGTATG GCTTGGAAAG AATTATGAGT GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA
    GCCATCGATC CTGAGCACCC AAGTATGATG ACAGATGTGG TGAAACTCCT CTCTGCGGTA
    TGCATTGTAG GAGAGGAAAG CATTCTCGAA GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA
    GAAGAAAGAA AAATCGACAG ATTTTCATCC ATTGTGGAAG GTCTCCGGCA TGATTCAGTT
    CAACTGCAAG TAGCGTGTAT GCAGCTCATC AATGCCCTTG TTACATCTCC TGATGATTTG
    GACTTCAGGC TTCACATCAG AAATGAATTT ATGCGTTGTG GATTGAAAGA GATCTTGCCA
    GATTTAAAAT GTATGAAGAA TGATAGCCTG GATATCCAAC TTAAAGTCTT TGATGAGCAT
    AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT CGCCTTGAAG ATATTAGAGC TGAACTTGAT
    GAAGCATATG ATATTTACAA CATGGTGTGG AACTCAGTTA AGGAAACTAA AGCAGAGGGA
    TATTTCATTT CTATTCTTCA GCACCTTTTG CTGATTCGAA ATGATTATAG TATAAGGCAA
    CAGTACTTCA AATTAATTGA TGAGTGTATA TCTCAGATTG TATTGCATAG AGATGGGATG
    GATCCAGACT TCACATATCG GAAAAGACTG GATTTAGATT TAAGTCAGTT TGTTGATACT
    TGCATAGATC AAGCAAAACT AGAAGAGTTT GAAGAGAAAG CGTCAGAACT GCACAAGAAA
    TTTGAAAAAG AGTTTACCGA CCACCAAGAA ACTCAGGCTC AATTGCAGAA AAAAGAAGCA
    AAGATTAATG AACTTCAAGC AGAGTTACAA GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA
    GCTGGTAGAA ATCACTCTTT GCCACAGTCA AAAGAAAATG GAACTGGCCC CCCAGCACTT
    GCTCCTCCCC TGCCACTGCC ATCTTGTGGA GGCATGCCGC CTCCACCCCC TCCTCCCCCA
    CCTCCTCCGC TTCCAGGAAT GCCAATGCCT TTTGGTGGTC CCGTGCCTCC TCCACCACCT
    CCTCTGGGAT TTCTCAGTGG GCGAAACTCT CCTCCTCCAC CAACCCTACC ATTTGGGTTA
    AAACCAAAGA AAGAATTTAA ACCTGAAACC AGCATGAGAA GATTGAATTG GTTAAAGATC
    AGACCTCACG AAATGACTGA AAGCTGTTTC TGGATCAAAG TAAATGAAAA TAAGTATGAA
    AATGTGGATT TGCTCTGTAA ACTTGAGAAT ACGTTTTGTT GCCAACAAAA AGAGAGAAGA
    GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT ATTAAGAAAA AAATTAAAGA GCTTAAATTT
    TTAGATTCTA AAATTGCCCA GAATCTTTCC ATCTTCCTGA GCTCTTTTCG GGTGCCATAT
    GAGGAAATCA AAACGATGAT ATTGGAAGTG GATGAAACAC AGTTGGCAGA GTCTATGATT
    CAGAACCTAA TAAAGCATCT TCCTGATCAA GAACAATTAA GCTCACTGTC TCAGTTCAAG
    AATGACTATA ACAACCTATG TGAACCCGAG CAGTTTGCTG TTGTGATGAG CAATGTGAAG
    AGACTACGGC CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC
    AACATCAAAC CTGACATCAT GGCTGTCAGT ACTGCCTGCG AGGAGATAAA GAAGAGCAAA
    AGCTTCAGCA AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC
    CGGAATGCTC AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACGAAA
    TCAGCAGATC AGAAAACAAC ACTACTTCAT TTTCTGGTGG AAGTATGTGA AGAAAAGTAT
    CCTGATATCC TGAATTTTGT GGATGATTTG GAACATTTAG ACAAAGCTAG TAAAGTCTCT
    GTAGAAACGC TGGAAAAGAA TTTGAAGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAA
    GATTTGGAAA CCTTTCCCCC TCCTGAGGAC TTGCATGATA AGTTTGTGAC AAAGATGTCC
    AGCTTTGTTA TCAGCGCAAA AGAACAATAT GAGAAACTTC TAAAGTTACA TGAAAATATG
    GAGAAGTTAT ACCAGAGTGT GATGGGATAC TATGCCATTG ACGTGAAGAA GATGTCCGTG
    GAAGACTTTT TTAATGACTT GAATAATTTC AGAACCACAT TCATGCAAGC AAGAAAGGAG
    AACATCAAAA AAAGAGAAGC AGAGGAAAGG GAAAAACGTG CCCAAATAGC CAAAGAATTG
    GCAGAGAAAG AAAGACTTGA ACGCCAACAA AAGAAAAAGC GCTTGTTAGA AATGAAGACT
    GAGGGTGATG AGACAGGAGT GATGGATAGT CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC
    TTCCGCGACC GAAGAAAAAG GACGCCAAAG CCAAAAGATA TTCGGCAAAG TCTCAGTCCC
    ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT AACCATGAAA ATCAGAAAGT GCAGTTGACA
    GAAGGGTCAC GTTCACACTA CAATATCAAT TGCAACTCAA CGAGGACTCC AGTCGCCAAG
    GAGCTTAATT ATAATCTAGA CACTCATACG TCTACAGGGA GGATCAAGGC AGTTGAGAAG
    AAGGAAGCCT GTAATGTAGA AAGCAACAGA AAAAAGGAAA TGGAACTTCT TGGCTCGGTT
    TCTAAAAACG AATCTGTTCC CGAAGTTGAA GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23596
    Clone ID Related Accession (Same CDS sequence) XM_005633947.2 , XM_005633948.3 , XM_005633948.2 , XM_005633947.3 , XM_005633946.2
    Accession Version XM_005633948.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633948.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTAACGG CTGAACATTT CCACCTCAGG CAGGGGACAA GTTGGGTAGA AAGCTTTGGA 
    CGCGAGGGGC TTGGATTATT ACTGGACATT TTGGAAAGAC TGATTAGTGG CAAAATACAA
    GAGAAAATTG TAAAGAAGAA TCAGCATAAA GTCATACAGT GTCTAAAGGC TTTGATGAAT
    ACACAGTATG GCTTGGAAAG AATTATGAGT GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA
    GCCATCGATC CTGAGCACCC AAGTATGATG ACAGATGTGG TGAAACTCCT CTCTGCGGTA
    TGCATTGTAG GAGAGGAAAG CATTCTCGAA GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA
    GAAGAAAGAA AAATCGACAG ATTTTCATCC ATTGTGGAAG GTCTCCGGCA TGATTCAGTT
    CAACTGCAAG TAGCGTGTAT GCAGCTCATC AATGCCCTTG TTACATCTCC TGATGATTTG
    GACTTCAGGC TTCACATCAG AAATGAATTT ATGCGTTGTG GATTGAAAGA GATCTTGCCA
    GATTTAAAAT GTATGAAGAA TGATAGCCTG GATATCCAAC TTAAAGTCTT TGATGAGCAT
    AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT CGCCTTGAAG ATATTAGAGC TGAACTTGAT
    GAAGCATATG ATATTTACAA CATGGTGTGG AACTCAGTTA AGGAAACTAA AGCAGAGGGA
    TATTTCATTT CTATTCTTCA GCACCTTTTG CTGATTCGAA ATGATTATAG TATAAGGCAA
    CAGTACTTCA AATTAATTGA TGAGTGTATA TCTCAGATTG TATTGCATAG AGATGGGATG
    GATCCAGACT TCACATATCG GAAAAGACTG GATTTAGATT TAAGTCAGTT TGTTGATACT
    TGCATAGATC AAGCAAAACT AGAAGAGTTT GAAGAGAAAG CGTCAGAACT GCACAAGAAA
    TTTGAAAAAG AGTTTACCGA CCACCAAGAA ACTCAGGCTC AATTGCAGAA AAAAGAAGCA
    AAGATTAATG AACTTCAAGC AGAGTTACAA GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA
    GCTGGTAGAA ATCACTCTTT GCCACAGTCA AAAGAAAATG GAACTGGCCC CCCAGCACTT
    GCTCCTCCCC TGCCACTGCC ATCTTGTGGA GGCATGCCGC CTCCACCCCC TCCTCCCCCA
    CCTCCTCCGC TTCCAGGAAT GCCAATGCCT TTTGGTGGTC CCGTGCCTCC TCCACCACCT
    CCTCTGGGAT TTCTCAGTGG GCGAAACTCT CCTCCTCCAC CAACCCTACC ATTTGGGTTA
    AAACCAAAGA AAGAATTTAA ACCTGAAACC AGCATGAGAA GATTGAATTG GTTAAAGATC
    AGACCTCACG AAATGACTGA AAGCTGTTTC TGGATCAAAG TAAATGAAAA TAAGTATGAA
    AATGTGGATT TGCTCTGTAA ACTTGAGAAT ACGTTTTGTT GCCAACAAAA AGAGAGAAGA
    GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT ATTAAGAAAA AAATTAAAGA GCTTAAATTT
    TTAGATTCTA AAATTGCCCA GAATCTTTCC ATCTTCCTGA GCTCTTTTCG GGTGCCATAT
    GAGGAAATCA AAACGATGAT ATTGGAAGTG GATGAAACAC AGTTGGCAGA GTCTATGATT
    CAGAACCTAA TAAAGCATCT TCCTGATCAA GAACAATTAA GCTCACTGTC TCAGTTCAAG
    AATGACTATA ACAACCTATG TGAACCCGAG CAGTTTGCTG TTGTGATGAG CAATGTGAAG
    AGACTACGGC CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC
    AACATCAAAC CTGACATCAT GGCTGTCAGT ACTGCCTGCG AGGAGATAAA GAAGAGCAAA
    AGCTTCAGCA AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC
    CGGAATGCTC AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACGAAA
    TCAGCAGATC AGAAAACAAC ACTACTTCAT TTTCTGGTGG AAGTATGTGA AGAAAAGTAT
    CCTGATATCC TGAATTTTGT GGATGATTTG GAACATTTAG ACAAAGCTAG TAAAGTCTCT
    GTAGAAACGC TGGAAAAGAA TTTGAAGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAA
    GATTTGGAAA CCTTTCCCCC TCCTGAGGAC TTGCATGATA AGTTTGTGAC AAAGATGTCC
    AGCTTTGTTA TCAGCGCAAA AGAACAATAT GAGAAACTTC TAAAGTTACA TGAAAATATG
    GAGAAGTTAT ACCAGAGTGT GATGGGATAC TATGCCATTG ACGTGAAGAA GATGTCCGTG
    GAAGACTTTT TTAATGACTT GAATAATTTC AGAACCACAT TCATGCAAGC AAGAAAGGAG
    AACATCAAAA AAAGAGAAGC AGAGGAAAGG GAAAAACGTG CCCAAATAGC CAAAGAATTG
    GCAGAGAAAG AAAGACTTGA ACGCCAACAA AAGAAAAAGC GCTTGTTAGA AATGAAGACT
    GAGGGTGATG AGACAGGAGT GATGGATAGT CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC
    TTCCGCGACC GAAGAAAAAG GACGCCAAAG CCAAAAGATA TTCGGCAAAG TCTCAGTCCC
    ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT AACCATGAAA ATCAGAAAGT GCAGTTGACA
    GAAGGGTCAC GTTCACACTA CAATATCAAT TGCAACTCAA CGAGGACTCC AGTCGCCAAG
    GAGCTTAATT ATAATCTAGA CACTCATACG TCTACAGGGA GGATCAAGGC AGTTGAGAAG
    AAGGAAGCCT GTAATGTAGA AAGCAACAGA AAAAAGGAAA TGGAACTTCT TGGCTCGGTT
    TCTAAAAACG AATCTGTTCC CGAAGTTGAA GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634005.1
    CDS122..3121
    Translation

    Target ORF information:

    RefSeq Version XM_005633948.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633948.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTAACGG CTGAACATTT CCACCTCAGG CAGGGGACAA GTTGGGTAGA AAGCTTTGGA 
    CGCGAGGGGC TTGGATTATT ACTGGACATT TTGGAAAGAC TGATTAGTGG CAAAATACAA
    GAGAAAATTG TAAAGAAGAA TCAGCATAAA GTCATACAGT GTCTAAAGGC TTTGATGAAT
    ACACAGTATG GCTTGGAAAG AATTATGAGT GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA
    GCCATCGATC CTGAGCACCC AAGTATGATG ACAGATGTGG TGAAACTCCT CTCTGCGGTA
    TGCATTGTAG GAGAGGAAAG CATTCTCGAA GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA
    GAAGAAAGAA AAATCGACAG ATTTTCATCC ATTGTGGAAG GTCTCCGGCA TGATTCAGTT
    CAACTGCAAG TAGCGTGTAT GCAGCTCATC AATGCCCTTG TTACATCTCC TGATGATTTG
    GACTTCAGGC TTCACATCAG AAATGAATTT ATGCGTTGTG GATTGAAAGA GATCTTGCCA
    GATTTAAAAT GTATGAAGAA TGATAGCCTG GATATCCAAC TTAAAGTCTT TGATGAGCAT
    AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT CGCCTTGAAG ATATTAGAGC TGAACTTGAT
    GAAGCATATG ATATTTACAA CATGGTGTGG AACTCAGTTA AGGAAACTAA AGCAGAGGGA
    TATTTCATTT CTATTCTTCA GCACCTTTTG CTGATTCGAA ATGATTATAG TATAAGGCAA
    CAGTACTTCA AATTAATTGA TGAGTGTATA TCTCAGATTG TATTGCATAG AGATGGGATG
    GATCCAGACT TCACATATCG GAAAAGACTG GATTTAGATT TAAGTCAGTT TGTTGATACT
    TGCATAGATC AAGCAAAACT AGAAGAGTTT GAAGAGAAAG CGTCAGAACT GCACAAGAAA
    TTTGAAAAAG AGTTTACCGA CCACCAAGAA ACTCAGGCTC AATTGCAGAA AAAAGAAGCA
    AAGATTAATG AACTTCAAGC AGAGTTACAA GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA
    GCTGGTAGAA ATCACTCTTT GCCACAGTCA AAAGAAAATG GAACTGGCCC CCCAGCACTT
    GCTCCTCCCC TGCCACTGCC ATCTTGTGGA GGCATGCCGC CTCCACCCCC TCCTCCCCCA
    CCTCCTCCGC TTCCAGGAAT GCCAATGCCT TTTGGTGGTC CCGTGCCTCC TCCACCACCT
    CCTCTGGGAT TTCTCAGTGG GCGAAACTCT CCTCCTCCAC CAACCCTACC ATTTGGGTTA
    AAACCAAAGA AAGAATTTAA ACCTGAAACC AGCATGAGAA GATTGAATTG GTTAAAGATC
    AGACCTCACG AAATGACTGA AAGCTGTTTC TGGATCAAAG TAAATGAAAA TAAGTATGAA
    AATGTGGATT TGCTCTGTAA ACTTGAGAAT ACGTTTTGTT GCCAACAAAA AGAGAGAAGA
    GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT ATTAAGAAAA AAATTAAAGA GCTTAAATTT
    TTAGATTCTA AAATTGCCCA GAATCTTTCC ATCTTCCTGA GCTCTTTTCG GGTGCCATAT
    GAGGAAATCA AAACGATGAT ATTGGAAGTG GATGAAACAC AGTTGGCAGA GTCTATGATT
    CAGAACCTAA TAAAGCATCT TCCTGATCAA GAACAATTAA GCTCACTGTC TCAGTTCAAG
    AATGACTATA ACAACCTATG TGAACCCGAG CAGTTTGCTG TTGTGATGAG CAATGTGAAG
    AGACTACGGC CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC
    AACATCAAAC CTGACATCAT GGCTGTCAGT ACTGCCTGCG AGGAGATAAA GAAGAGCAAA
    AGCTTCAGCA AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC
    CGGAATGCTC AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACGAAA
    TCAGCAGATC AGAAAACAAC ACTACTTCAT TTTCTGGTGG AAGTATGTGA AGAAAAGTAT
    CCTGATATCC TGAATTTTGT GGATGATTTG GAACATTTAG ACAAAGCTAG TAAAGTCTCT
    GTAGAAACGC TGGAAAAGAA TTTGAAGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAA
    GATTTGGAAA CCTTTCCCCC TCCTGAGGAC TTGCATGATA AGTTTGTGAC AAAGATGTCC
    AGCTTTGTTA TCAGCGCAAA AGAACAATAT GAGAAACTTC TAAAGTTACA TGAAAATATG
    GAGAAGTTAT ACCAGAGTGT GATGGGATAC TATGCCATTG ACGTGAAGAA GATGTCCGTG
    GAAGACTTTT TTAATGACTT GAATAATTTC AGAACCACAT TCATGCAAGC AAGAAAGGAG
    AACATCAAAA AAAGAGAAGC AGAGGAAAGG GAAAAACGTG CCCAAATAGC CAAAGAATTG
    GCAGAGAAAG AAAGACTTGA ACGCCAACAA AAGAAAAAGC GCTTGTTAGA AATGAAGACT
    GAGGGTGATG AGACAGGAGT GATGGATAGT CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC
    TTCCGCGACC GAAGAAAAAG GACGCCAAAG CCAAAAGATA TTCGGCAAAG TCTCAGTCCC
    ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT AACCATGAAA ATCAGAAAGT GCAGTTGACA
    GAAGGGTCAC GTTCACACTA CAATATCAAT TGCAACTCAA CGAGGACTCC AGTCGCCAAG
    GAGCTTAATT ATAATCTAGA CACTCATACG TCTACAGGGA GGATCAAGGC AGTTGAGAAG
    AAGGAAGCCT GTAATGTAGA AAGCAACAGA AAAAAGGAAA TGGAACTTCT TGGCTCGGTT
    TCTAAAAACG AATCTGTTCC CGAAGTTGAA GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23596
    Clone ID Related Accession (Same CDS sequence) XM_005633947.2 , XM_005633948.3 , XM_005633948.2 , XM_005633947.3 , XM_005633946.2
    Accession Version XM_005633948.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein diaphanous homolog 3 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633948.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTAACGG CTGAACATTT CCACCTCAGG CAGGGGACAA GTTGGGTAGA AAGCTTTGGA 
    CGCGAGGGGC TTGGATTATT ACTGGACATT TTGGAAAGAC TGATTAGTGG CAAAATACAA
    GAGAAAATTG TAAAGAAGAA TCAGCATAAA GTCATACAGT GTCTAAAGGC TTTGATGAAT
    ACACAGTATG GCTTGGAAAG AATTATGAGT GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA
    GCCATCGATC CTGAGCACCC AAGTATGATG ACAGATGTGG TGAAACTCCT CTCTGCGGTA
    TGCATTGTAG GAGAGGAAAG CATTCTCGAA GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA
    GAAGAAAGAA AAATCGACAG ATTTTCATCC ATTGTGGAAG GTCTCCGGCA TGATTCAGTT
    CAACTGCAAG TAGCGTGTAT GCAGCTCATC AATGCCCTTG TTACATCTCC TGATGATTTG
    GACTTCAGGC TTCACATCAG AAATGAATTT ATGCGTTGTG GATTGAAAGA GATCTTGCCA
    GATTTAAAAT GTATGAAGAA TGATAGCCTG GATATCCAAC TTAAAGTCTT TGATGAGCAT
    AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT CGCCTTGAAG ATATTAGAGC TGAACTTGAT
    GAAGCATATG ATATTTACAA CATGGTGTGG AACTCAGTTA AGGAAACTAA AGCAGAGGGA
    TATTTCATTT CTATTCTTCA GCACCTTTTG CTGATTCGAA ATGATTATAG TATAAGGCAA
    CAGTACTTCA AATTAATTGA TGAGTGTATA TCTCAGATTG TATTGCATAG AGATGGGATG
    GATCCAGACT TCACATATCG GAAAAGACTG GATTTAGATT TAAGTCAGTT TGTTGATACT
    TGCATAGATC AAGCAAAACT AGAAGAGTTT GAAGAGAAAG CGTCAGAACT GCACAAGAAA
    TTTGAAAAAG AGTTTACCGA CCACCAAGAA ACTCAGGCTC AATTGCAGAA AAAAGAAGCA
    AAGATTAATG AACTTCAAGC AGAGTTACAA GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA
    GCTGGTAGAA ATCACTCTTT GCCACAGTCA AAAGAAAATG GAACTGGCCC CCCAGCACTT
    GCTCCTCCCC TGCCACTGCC ATCTTGTGGA GGCATGCCGC CTCCACCCCC TCCTCCCCCA
    CCTCCTCCGC TTCCAGGAAT GCCAATGCCT TTTGGTGGTC CCGTGCCTCC TCCACCACCT
    CCTCTGGGAT TTCTCAGTGG GCGAAACTCT CCTCCTCCAC CAACCCTACC ATTTGGGTTA
    AAACCAAAGA AAGAATTTAA ACCTGAAACC AGCATGAGAA GATTGAATTG GTTAAAGATC
    AGACCTCACG AAATGACTGA AAGCTGTTTC TGGATCAAAG TAAATGAAAA TAAGTATGAA
    AATGTGGATT TGCTCTGTAA ACTTGAGAAT ACGTTTTGTT GCCAACAAAA AGAGAGAAGA
    GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT ATTAAGAAAA AAATTAAAGA GCTTAAATTT
    TTAGATTCTA AAATTGCCCA GAATCTTTCC ATCTTCCTGA GCTCTTTTCG GGTGCCATAT
    GAGGAAATCA AAACGATGAT ATTGGAAGTG GATGAAACAC AGTTGGCAGA GTCTATGATT
    CAGAACCTAA TAAAGCATCT TCCTGATCAA GAACAATTAA GCTCACTGTC TCAGTTCAAG
    AATGACTATA ACAACCTATG TGAACCCGAG CAGTTTGCTG TTGTGATGAG CAATGTGAAG
    AGACTACGGC CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC
    AACATCAAAC CTGACATCAT GGCTGTCAGT ACTGCCTGCG AGGAGATAAA GAAGAGCAAA
    AGCTTCAGCA AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC
    CGGAATGCTC AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACGAAA
    TCAGCAGATC AGAAAACAAC ACTACTTCAT TTTCTGGTGG AAGTATGTGA AGAAAAGTAT
    CCTGATATCC TGAATTTTGT GGATGATTTG GAACATTTAG ACAAAGCTAG TAAAGTCTCT
    GTAGAAACGC TGGAAAAGAA TTTGAAGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAA
    GATTTGGAAA CCTTTCCCCC TCCTGAGGAC TTGCATGATA AGTTTGTGAC AAAGATGTCC
    AGCTTTGTTA TCAGCGCAAA AGAACAATAT GAGAAACTTC TAAAGTTACA TGAAAATATG
    GAGAAGTTAT ACCAGAGTGT GATGGGATAC TATGCCATTG ACGTGAAGAA GATGTCCGTG
    GAAGACTTTT TTAATGACTT GAATAATTTC AGAACCACAT TCATGCAAGC AAGAAAGGAG
    AACATCAAAA AAAGAGAAGC AGAGGAAAGG GAAAAACGTG CCCAAATAGC CAAAGAATTG
    GCAGAGAAAG AAAGACTTGA ACGCCAACAA AAGAAAAAGC GCTTGTTAGA AATGAAGACT
    GAGGGTGATG AGACAGGAGT GATGGATAGT CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC
    TTCCGCGACC GAAGAAAAAG GACGCCAAAG CCAAAAGATA TTCGGCAAAG TCTCAGTCCC
    ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT AACCATGAAA ATCAGAAAGT GCAGTTGACA
    GAAGGGTCAC GTTCACACTA CAATATCAAT TGCAACTCAA CGAGGACTCC AGTCGCCAAG
    GAGCTTAATT ATAATCTAGA CACTCATACG TCTACAGGGA GGATCAAGGC AGTTGAGAAG
    AAGGAAGCCT GTAATGTAGA AAGCAACAGA AAAAAGGAAA TGGAACTTCT TGGCTCGGTT
    TCTAAAAACG AATCTGTTCC CGAAGTTGAA GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634005.1
    CDS149..3148
    Translation

    Target ORF information:

    RefSeq Version XM_005633948.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633948.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTAACGG CTGAACATTT CCACCTCAGG CAGGGGACAA GTTGGGTAGA AAGCTTTGGA 
    CGCGAGGGGC TTGGATTATT ACTGGACATT TTGGAAAGAC TGATTAGTGG CAAAATACAA
    GAGAAAATTG TAAAGAAGAA TCAGCATAAA GTCATACAGT GTCTAAAGGC TTTGATGAAT
    ACACAGTATG GCTTGGAAAG AATTATGAGT GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA
    GCCATCGATC CTGAGCACCC AAGTATGATG ACAGATGTGG TGAAACTCCT CTCTGCGGTA
    TGCATTGTAG GAGAGGAAAG CATTCTCGAA GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA
    GAAGAAAGAA AAATCGACAG ATTTTCATCC ATTGTGGAAG GTCTCCGGCA TGATTCAGTT
    CAACTGCAAG TAGCGTGTAT GCAGCTCATC AATGCCCTTG TTACATCTCC TGATGATTTG
    GACTTCAGGC TTCACATCAG AAATGAATTT ATGCGTTGTG GATTGAAAGA GATCTTGCCA
    GATTTAAAAT GTATGAAGAA TGATAGCCTG GATATCCAAC TTAAAGTCTT TGATGAGCAT
    AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT CGCCTTGAAG ATATTAGAGC TGAACTTGAT
    GAAGCATATG ATATTTACAA CATGGTGTGG AACTCAGTTA AGGAAACTAA AGCAGAGGGA
    TATTTCATTT CTATTCTTCA GCACCTTTTG CTGATTCGAA ATGATTATAG TATAAGGCAA
    CAGTACTTCA AATTAATTGA TGAGTGTATA TCTCAGATTG TATTGCATAG AGATGGGATG
    GATCCAGACT TCACATATCG GAAAAGACTG GATTTAGATT TAAGTCAGTT TGTTGATACT
    TGCATAGATC AAGCAAAACT AGAAGAGTTT GAAGAGAAAG CGTCAGAACT GCACAAGAAA
    TTTGAAAAAG AGTTTACCGA CCACCAAGAA ACTCAGGCTC AATTGCAGAA AAAAGAAGCA
    AAGATTAATG AACTTCAAGC AGAGTTACAA GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA
    GCTGGTAGAA ATCACTCTTT GCCACAGTCA AAAGAAAATG GAACTGGCCC CCCAGCACTT
    GCTCCTCCCC TGCCACTGCC ATCTTGTGGA GGCATGCCGC CTCCACCCCC TCCTCCCCCA
    CCTCCTCCGC TTCCAGGAAT GCCAATGCCT TTTGGTGGTC CCGTGCCTCC TCCACCACCT
    CCTCTGGGAT TTCTCAGTGG GCGAAACTCT CCTCCTCCAC CAACCCTACC ATTTGGGTTA
    AAACCAAAGA AAGAATTTAA ACCTGAAACC AGCATGAGAA GATTGAATTG GTTAAAGATC
    AGACCTCACG AAATGACTGA AAGCTGTTTC TGGATCAAAG TAAATGAAAA TAAGTATGAA
    AATGTGGATT TGCTCTGTAA ACTTGAGAAT ACGTTTTGTT GCCAACAAAA AGAGAGAAGA
    GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT ATTAAGAAAA AAATTAAAGA GCTTAAATTT
    TTAGATTCTA AAATTGCCCA GAATCTTTCC ATCTTCCTGA GCTCTTTTCG GGTGCCATAT
    GAGGAAATCA AAACGATGAT ATTGGAAGTG GATGAAACAC AGTTGGCAGA GTCTATGATT
    CAGAACCTAA TAAAGCATCT TCCTGATCAA GAACAATTAA GCTCACTGTC TCAGTTCAAG
    AATGACTATA ACAACCTATG TGAACCCGAG CAGTTTGCTG TTGTGATGAG CAATGTGAAG
    AGACTACGGC CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC
    AACATCAAAC CTGACATCAT GGCTGTCAGT ACTGCCTGCG AGGAGATAAA GAAGAGCAAA
    AGCTTCAGCA AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC
    CGGAATGCTC AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACGAAA
    TCAGCAGATC AGAAAACAAC ACTACTTCAT TTTCTGGTGG AAGTATGTGA AGAAAAGTAT
    CCTGATATCC TGAATTTTGT GGATGATTTG GAACATTTAG ACAAAGCTAG TAAAGTCTCT
    GTAGAAACGC TGGAAAAGAA TTTGAAGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAA
    GATTTGGAAA CCTTTCCCCC TCCTGAGGAC TTGCATGATA AGTTTGTGAC AAAGATGTCC
    AGCTTTGTTA TCAGCGCAAA AGAACAATAT GAGAAACTTC TAAAGTTACA TGAAAATATG
    GAGAAGTTAT ACCAGAGTGT GATGGGATAC TATGCCATTG ACGTGAAGAA GATGTCCGTG
    GAAGACTTTT TTAATGACTT GAATAATTTC AGAACCACAT TCATGCAAGC AAGAAAGGAG
    AACATCAAAA AAAGAGAAGC AGAGGAAAGG GAAAAACGTG CCCAAATAGC CAAAGAATTG
    GCAGAGAAAG AAAGACTTGA ACGCCAACAA AAGAAAAAGC GCTTGTTAGA AATGAAGACT
    GAGGGTGATG AGACAGGAGT GATGGATAGT CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC
    TTCCGCGACC GAAGAAAAAG GACGCCAAAG CCAAAAGATA TTCGGCAAAG TCTCAGTCCC
    ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT AACCATGAAA ATCAGAAAGT GCAGTTGACA
    GAAGGGTCAC GTTCACACTA CAATATCAAT TGCAACTCAA CGAGGACTCC AGTCGCCAAG
    GAGCTTAATT ATAATCTAGA CACTCATACG TCTACAGGGA GGATCAAGGC AGTTGAGAAG
    AAGGAAGCCT GTAATGTAGA AAGCAACAGA AAAAAGGAAA TGGAACTTCT TGGCTCGGTT
    TCTAAAAACG AATCTGTTCC CGAAGTTGAA GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23596
    Clone ID Related Accession (Same CDS sequence) XM_005633947.2 , XM_005633948.3 , XM_005633948.2 , XM_005633947.3 , XM_005633946.2
    Accession Version XM_005633947.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633947.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTAACGG CTGAACATTT CCACCTCAGG CAGGGGACAA GTTGGGTAGA AAGCTTTGGA 
    CGCGAGGGGC TTGGATTATT ACTGGACATT TTGGAAAGAC TGATTAGTGG CAAAATACAA
    GAGAAAATTG TAAAGAAGAA TCAGCATAAA GTCATACAGT GTCTAAAGGC TTTGATGAAT
    ACACAGTATG GCTTGGAAAG AATTATGAGT GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA
    GCCATCGATC CTGAGCACCC AAGTATGATG ACAGATGTGG TGAAACTCCT CTCTGCGGTA
    TGCATTGTAG GAGAGGAAAG CATTCTCGAA GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA
    GAAGAAAGAA AAATCGACAG ATTTTCATCC ATTGTGGAAG GTCTCCGGCA TGATTCAGTT
    CAACTGCAAG TAGCGTGTAT GCAGCTCATC AATGCCCTTG TTACATCTCC TGATGATTTG
    GACTTCAGGC TTCACATCAG AAATGAATTT ATGCGTTGTG GATTGAAAGA GATCTTGCCA
    GATTTAAAAT GTATGAAGAA TGATAGCCTG GATATCCAAC TTAAAGTCTT TGATGAGCAT
    AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT CGCCTTGAAG ATATTAGAGC TGAACTTGAT
    GAAGCATATG ATATTTACAA CATGGTGTGG AACTCAGTTA AGGAAACTAA AGCAGAGGGA
    TATTTCATTT CTATTCTTCA GCACCTTTTG CTGATTCGAA ATGATTATAG TATAAGGCAA
    CAGTACTTCA AATTAATTGA TGAGTGTATA TCTCAGATTG TATTGCATAG AGATGGGATG
    GATCCAGACT TCACATATCG GAAAAGACTG GATTTAGATT TAAGTCAGTT TGTTGATACT
    TGCATAGATC AAGCAAAACT AGAAGAGTTT GAAGAGAAAG CGTCAGAACT GCACAAGAAA
    TTTGAAAAAG AGTTTACCGA CCACCAAGAA ACTCAGGCTC AATTGCAGAA AAAAGAAGCA
    AAGATTAATG AACTTCAAGC AGAGTTACAA GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA
    GCTGGTAGAA ATCACTCTTT GCCACAGTCA AAAGAAAATG GAACTGGCCC CCCAGCACTT
    GCTCCTCCCC TGCCACTGCC ATCTTGTGGA GGCATGCCGC CTCCACCCCC TCCTCCCCCA
    CCTCCTCCGC TTCCAGGAAT GCCAATGCCT TTTGGTGGTC CCGTGCCTCC TCCACCACCT
    CCTCTGGGAT TTCTCAGTGG GCGAAACTCT CCTCCTCCAC CAACCCTACC ATTTGGGTTA
    AAACCAAAGA AAGAATTTAA ACCTGAAACC AGCATGAGAA GATTGAATTG GTTAAAGATC
    AGACCTCACG AAATGACTGA AAGCTGTTTC TGGATCAAAG TAAATGAAAA TAAGTATGAA
    AATGTGGATT TGCTCTGTAA ACTTGAGAAT ACGTTTTGTT GCCAACAAAA AGAGAGAAGA
    GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT ATTAAGAAAA AAATTAAAGA GCTTAAATTT
    TTAGATTCTA AAATTGCCCA GAATCTTTCC ATCTTCCTGA GCTCTTTTCG GGTGCCATAT
    GAGGAAATCA AAACGATGAT ATTGGAAGTG GATGAAACAC AGTTGGCAGA GTCTATGATT
    CAGAACCTAA TAAAGCATCT TCCTGATCAA GAACAATTAA GCTCACTGTC TCAGTTCAAG
    AATGACTATA ACAACCTATG TGAACCCGAG CAGTTTGCTG TTGTGATGAG CAATGTGAAG
    AGACTACGGC CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC
    AACATCAAAC CTGACATCAT GGCTGTCAGT ACTGCCTGCG AGGAGATAAA GAAGAGCAAA
    AGCTTCAGCA AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC
    CGGAATGCTC AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACGAAA
    TCAGCAGATC AGAAAACAAC ACTACTTCAT TTTCTGGTGG AAGTATGTGA AGAAAAGTAT
    CCTGATATCC TGAATTTTGT GGATGATTTG GAACATTTAG ACAAAGCTAG TAAAGTCTCT
    GTAGAAACGC TGGAAAAGAA TTTGAAGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAA
    GATTTGGAAA CCTTTCCCCC TCCTGAGGAC TTGCATGATA AGTTTGTGAC AAAGATGTCC
    AGCTTTGTTA TCAGCGCAAA AGAACAATAT GAGAAACTTC TAAAGTTACA TGAAAATATG
    GAGAAGTTAT ACCAGAGTGT GATGGGATAC TATGCCATTG ACGTGAAGAA GATGTCCGTG
    GAAGACTTTT TTAATGACTT GAATAATTTC AGAACCACAT TCATGCAAGC AAGAAAGGAG
    AACATCAAAA AAAGAGAAGC AGAGGAAAGG GAAAAACGTG CCCAAATAGC CAAAGAATTG
    GCAGAGAAAG AAAGACTTGA ACGCCAACAA AAGAAAAAGC GCTTGTTAGA AATGAAGACT
    GAGGGTGATG AGACAGGAGT GATGGATAGT CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC
    TTCCGCGACC GAAGAAAAAG GACGCCAAAG CCAAAAGATA TTCGGCAAAG TCTCAGTCCC
    ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT AACCATGAAA ATCAGAAAGT GCAGTTGACA
    GAAGGGTCAC GTTCACACTA CAATATCAAT TGCAACTCAA CGAGGACTCC AGTCGCCAAG
    GAGCTTAATT ATAATCTAGA CACTCATACG TCTACAGGGA GGATCAAGGC AGTTGAGAAG
    AAGGAAGCCT GTAATGTAGA AAGCAACAGA AAAAAGGAAA TGGAACTTCT TGGCTCGGTT
    TCTAAAAACG AATCTGTTCC CGAAGTTGAA GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634004.1
    CDS216..3215
    Translation

    Target ORF information:

    RefSeq Version XM_005633947.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633947.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTAACGG CTGAACATTT CCACCTCAGG CAGGGGACAA GTTGGGTAGA AAGCTTTGGA 
    CGCGAGGGGC TTGGATTATT ACTGGACATT TTGGAAAGAC TGATTAGTGG CAAAATACAA
    GAGAAAATTG TAAAGAAGAA TCAGCATAAA GTCATACAGT GTCTAAAGGC TTTGATGAAT
    ACACAGTATG GCTTGGAAAG AATTATGAGT GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA
    GCCATCGATC CTGAGCACCC AAGTATGATG ACAGATGTGG TGAAACTCCT CTCTGCGGTA
    TGCATTGTAG GAGAGGAAAG CATTCTCGAA GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA
    GAAGAAAGAA AAATCGACAG ATTTTCATCC ATTGTGGAAG GTCTCCGGCA TGATTCAGTT
    CAACTGCAAG TAGCGTGTAT GCAGCTCATC AATGCCCTTG TTACATCTCC TGATGATTTG
    GACTTCAGGC TTCACATCAG AAATGAATTT ATGCGTTGTG GATTGAAAGA GATCTTGCCA
    GATTTAAAAT GTATGAAGAA TGATAGCCTG GATATCCAAC TTAAAGTCTT TGATGAGCAT
    AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT CGCCTTGAAG ATATTAGAGC TGAACTTGAT
    GAAGCATATG ATATTTACAA CATGGTGTGG AACTCAGTTA AGGAAACTAA AGCAGAGGGA
    TATTTCATTT CTATTCTTCA GCACCTTTTG CTGATTCGAA ATGATTATAG TATAAGGCAA
    CAGTACTTCA AATTAATTGA TGAGTGTATA TCTCAGATTG TATTGCATAG AGATGGGATG
    GATCCAGACT TCACATATCG GAAAAGACTG GATTTAGATT TAAGTCAGTT TGTTGATACT
    TGCATAGATC AAGCAAAACT AGAAGAGTTT GAAGAGAAAG CGTCAGAACT GCACAAGAAA
    TTTGAAAAAG AGTTTACCGA CCACCAAGAA ACTCAGGCTC AATTGCAGAA AAAAGAAGCA
    AAGATTAATG AACTTCAAGC AGAGTTACAA GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA
    GCTGGTAGAA ATCACTCTTT GCCACAGTCA AAAGAAAATG GAACTGGCCC CCCAGCACTT
    GCTCCTCCCC TGCCACTGCC ATCTTGTGGA GGCATGCCGC CTCCACCCCC TCCTCCCCCA
    CCTCCTCCGC TTCCAGGAAT GCCAATGCCT TTTGGTGGTC CCGTGCCTCC TCCACCACCT
    CCTCTGGGAT TTCTCAGTGG GCGAAACTCT CCTCCTCCAC CAACCCTACC ATTTGGGTTA
    AAACCAAAGA AAGAATTTAA ACCTGAAACC AGCATGAGAA GATTGAATTG GTTAAAGATC
    AGACCTCACG AAATGACTGA AAGCTGTTTC TGGATCAAAG TAAATGAAAA TAAGTATGAA
    AATGTGGATT TGCTCTGTAA ACTTGAGAAT ACGTTTTGTT GCCAACAAAA AGAGAGAAGA
    GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT ATTAAGAAAA AAATTAAAGA GCTTAAATTT
    TTAGATTCTA AAATTGCCCA GAATCTTTCC ATCTTCCTGA GCTCTTTTCG GGTGCCATAT
    GAGGAAATCA AAACGATGAT ATTGGAAGTG GATGAAACAC AGTTGGCAGA GTCTATGATT
    CAGAACCTAA TAAAGCATCT TCCTGATCAA GAACAATTAA GCTCACTGTC TCAGTTCAAG
    AATGACTATA ACAACCTATG TGAACCCGAG CAGTTTGCTG TTGTGATGAG CAATGTGAAG
    AGACTACGGC CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC
    AACATCAAAC CTGACATCAT GGCTGTCAGT ACTGCCTGCG AGGAGATAAA GAAGAGCAAA
    AGCTTCAGCA AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC
    CGGAATGCTC AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACGAAA
    TCAGCAGATC AGAAAACAAC ACTACTTCAT TTTCTGGTGG AAGTATGTGA AGAAAAGTAT
    CCTGATATCC TGAATTTTGT GGATGATTTG GAACATTTAG ACAAAGCTAG TAAAGTCTCT
    GTAGAAACGC TGGAAAAGAA TTTGAAGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAA
    GATTTGGAAA CCTTTCCCCC TCCTGAGGAC TTGCATGATA AGTTTGTGAC AAAGATGTCC
    AGCTTTGTTA TCAGCGCAAA AGAACAATAT GAGAAACTTC TAAAGTTACA TGAAAATATG
    GAGAAGTTAT ACCAGAGTGT GATGGGATAC TATGCCATTG ACGTGAAGAA GATGTCCGTG
    GAAGACTTTT TTAATGACTT GAATAATTTC AGAACCACAT TCATGCAAGC AAGAAAGGAG
    AACATCAAAA AAAGAGAAGC AGAGGAAAGG GAAAAACGTG CCCAAATAGC CAAAGAATTG
    GCAGAGAAAG AAAGACTTGA ACGCCAACAA AAGAAAAAGC GCTTGTTAGA AATGAAGACT
    GAGGGTGATG AGACAGGAGT GATGGATAGT CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC
    TTCCGCGACC GAAGAAAAAG GACGCCAAAG CCAAAAGATA TTCGGCAAAG TCTCAGTCCC
    ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT AACCATGAAA ATCAGAAAGT GCAGTTGACA
    GAAGGGTCAC GTTCACACTA CAATATCAAT TGCAACTCAA CGAGGACTCC AGTCGCCAAG
    GAGCTTAATT ATAATCTAGA CACTCATACG TCTACAGGGA GGATCAAGGC AGTTGAGAAG
    AAGGAAGCCT GTAATGTAGA AAGCAACAGA AAAAAGGAAA TGGAACTTCT TGGCTCGGTT
    TCTAAAAACG AATCTGTTCC CGAAGTTGAA GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23596
    Clone ID Related Accession (Same CDS sequence) XM_005633947.2 , XM_005633948.3 , XM_005633948.2 , XM_005633947.3 , XM_005633946.2
    Accession Version XM_005633946.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein diaphanous homolog 3 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633946.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTAACGG CTGAACATTT CCACCTCAGG CAGGGGACAA GTTGGGTAGA AAGCTTTGGA 
    CGCGAGGGGC TTGGATTATT ACTGGACATT TTGGAAAGAC TGATTAGTGG CAAAATACAA
    GAGAAAATTG TAAAGAAGAA TCAGCATAAA GTCATACAGT GTCTAAAGGC TTTGATGAAT
    ACACAGTATG GCTTGGAAAG AATTATGAGT GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA
    GCCATCGATC CTGAGCACCC AAGTATGATG ACAGATGTGG TGAAACTCCT CTCTGCGGTA
    TGCATTGTAG GAGAGGAAAG CATTCTCGAA GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA
    GAAGAAAGAA AAATCGACAG ATTTTCATCC ATTGTGGAAG GTCTCCGGCA TGATTCAGTT
    CAACTGCAAG TAGCGTGTAT GCAGCTCATC AATGCCCTTG TTACATCTCC TGATGATTTG
    GACTTCAGGC TTCACATCAG AAATGAATTT ATGCGTTGTG GATTGAAAGA GATCTTGCCA
    GATTTAAAAT GTATGAAGAA TGATAGCCTG GATATCCAAC TTAAAGTCTT TGATGAGCAT
    AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT CGCCTTGAAG ATATTAGAGC TGAACTTGAT
    GAAGCATATG ATATTTACAA CATGGTGTGG AACTCAGTTA AGGAAACTAA AGCAGAGGGA
    TATTTCATTT CTATTCTTCA GCACCTTTTG CTGATTCGAA ATGATTATAG TATAAGGCAA
    CAGTACTTCA AATTAATTGA TGAGTGTATA TCTCAGATTG TATTGCATAG AGATGGGATG
    GATCCAGACT TCACATATCG GAAAAGACTG GATTTAGATT TAAGTCAGTT TGTTGATACT
    TGCATAGATC AAGCAAAACT AGAAGAGTTT GAAGAGAAAG CGTCAGAACT GCACAAGAAA
    TTTGAAAAAG AGTTTACCGA CCACCAAGAA ACTCAGGCTC AATTGCAGAA AAAAGAAGCA
    AAGATTAATG AACTTCAAGC AGAGTTACAA GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA
    GCTGGTAGAA ATCACTCTTT GCCACAGTCA AAAGAAAATG GAACTGGCCC CCCAGCACTT
    GCTCCTCCCC TGCCACTGCC ATCTTGTGGA GGCATGCCGC CTCCACCCCC TCCTCCCCCA
    CCTCCTCCGC TTCCAGGAAT GCCAATGCCT TTTGGTGGTC CCGTGCCTCC TCCACCACCT
    CCTCTGGGAT TTCTCAGTGG GCGAAACTCT CCTCCTCCAC CAACCCTACC ATTTGGGTTA
    AAACCAAAGA AAGAATTTAA ACCTGAAACC AGCATGAGAA GATTGAATTG GTTAAAGATC
    AGACCTCACG AAATGACTGA AAGCTGTTTC TGGATCAAAG TAAATGAAAA TAAGTATGAA
    AATGTGGATT TGCTCTGTAA ACTTGAGAAT ACGTTTTGTT GCCAACAAAA AGAGAGAAGA
    GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT ATTAAGAAAA AAATTAAAGA GCTTAAATTT
    TTAGATTCTA AAATTGCCCA GAATCTTTCC ATCTTCCTGA GCTCTTTTCG GGTGCCATAT
    GAGGAAATCA AAACGATGAT ATTGGAAGTG GATGAAACAC AGTTGGCAGA GTCTATGATT
    CAGAACCTAA TAAAGCATCT TCCTGATCAA GAACAATTAA GCTCACTGTC TCAGTTCAAG
    AATGACTATA ACAACCTATG TGAACCCGAG CAGTTTGCTG TTGTGATGAG CAATGTGAAG
    AGACTACGGC CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC
    AACATCAAAC CTGACATCAT GGCTGTCAGT ACTGCCTGCG AGGAGATAAA GAAGAGCAAA
    AGCTTCAGCA AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC
    CGGAATGCTC AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACGAAA
    TCAGCAGATC AGAAAACAAC ACTACTTCAT TTTCTGGTGG AAGTATGTGA AGAAAAGTAT
    CCTGATATCC TGAATTTTGT GGATGATTTG GAACATTTAG ACAAAGCTAG TAAAGTCTCT
    GTAGAAACGC TGGAAAAGAA TTTGAAGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAA
    GATTTGGAAA CCTTTCCCCC TCCTGAGGAC TTGCATGATA AGTTTGTGAC AAAGATGTCC
    AGCTTTGTTA TCAGCGCAAA AGAACAATAT GAGAAACTTC TAAAGTTACA TGAAAATATG
    GAGAAGTTAT ACCAGAGTGT GATGGGATAC TATGCCATTG ACGTGAAGAA GATGTCCGTG
    GAAGACTTTT TTAATGACTT GAATAATTTC AGAACCACAT TCATGCAAGC AAGAAAGGAG
    AACATCAAAA AAAGAGAAGC AGAGGAAAGG GAAAAACGTG CCCAAATAGC CAAAGAATTG
    GCAGAGAAAG AAAGACTTGA ACGCCAACAA AAGAAAAAGC GCTTGTTAGA AATGAAGACT
    GAGGGTGATG AGACAGGAGT GATGGATAGT CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC
    TTCCGCGACC GAAGAAAAAG GACGCCAAAG CCAAAAGATA TTCGGCAAAG TCTCAGTCCC
    ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT AACCATGAAA ATCAGAAAGT GCAGTTGACA
    GAAGGGTCAC GTTCACACTA CAATATCAAT TGCAACTCAA CGAGGACTCC AGTCGCCAAG
    GAGCTTAATT ATAATCTAGA CACTCATACG TCTACAGGGA GGATCAAGGC AGTTGAGAAG
    AAGGAAGCCT GTAATGTAGA AAGCAACAGA AAAAAGGAAA TGGAACTTCT TGGCTCGGTT
    TCTAAAAACG AATCTGTTCC CGAAGTTGAA GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634003.1
    CDS199..3198
    Translation

    Target ORF information:

    RefSeq Version XM_005633946.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633946.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTAACGG CTGAACATTT CCACCTCAGG CAGGGGACAA GTTGGGTAGA AAGCTTTGGA 
    CGCGAGGGGC TTGGATTATT ACTGGACATT TTGGAAAGAC TGATTAGTGG CAAAATACAA
    GAGAAAATTG TAAAGAAGAA TCAGCATAAA GTCATACAGT GTCTAAAGGC TTTGATGAAT
    ACACAGTATG GCTTGGAAAG AATTATGAGT GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA
    GCCATCGATC CTGAGCACCC AAGTATGATG ACAGATGTGG TGAAACTCCT CTCTGCGGTA
    TGCATTGTAG GAGAGGAAAG CATTCTCGAA GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA
    GAAGAAAGAA AAATCGACAG ATTTTCATCC ATTGTGGAAG GTCTCCGGCA TGATTCAGTT
    CAACTGCAAG TAGCGTGTAT GCAGCTCATC AATGCCCTTG TTACATCTCC TGATGATTTG
    GACTTCAGGC TTCACATCAG AAATGAATTT ATGCGTTGTG GATTGAAAGA GATCTTGCCA
    GATTTAAAAT GTATGAAGAA TGATAGCCTG GATATCCAAC TTAAAGTCTT TGATGAGCAT
    AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT CGCCTTGAAG ATATTAGAGC TGAACTTGAT
    GAAGCATATG ATATTTACAA CATGGTGTGG AACTCAGTTA AGGAAACTAA AGCAGAGGGA
    TATTTCATTT CTATTCTTCA GCACCTTTTG CTGATTCGAA ATGATTATAG TATAAGGCAA
    CAGTACTTCA AATTAATTGA TGAGTGTATA TCTCAGATTG TATTGCATAG AGATGGGATG
    GATCCAGACT TCACATATCG GAAAAGACTG GATTTAGATT TAAGTCAGTT TGTTGATACT
    TGCATAGATC AAGCAAAACT AGAAGAGTTT GAAGAGAAAG CGTCAGAACT GCACAAGAAA
    TTTGAAAAAG AGTTTACCGA CCACCAAGAA ACTCAGGCTC AATTGCAGAA AAAAGAAGCA
    AAGATTAATG AACTTCAAGC AGAGTTACAA GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA
    GCTGGTAGAA ATCACTCTTT GCCACAGTCA AAAGAAAATG GAACTGGCCC CCCAGCACTT
    GCTCCTCCCC TGCCACTGCC ATCTTGTGGA GGCATGCCGC CTCCACCCCC TCCTCCCCCA
    CCTCCTCCGC TTCCAGGAAT GCCAATGCCT TTTGGTGGTC CCGTGCCTCC TCCACCACCT
    CCTCTGGGAT TTCTCAGTGG GCGAAACTCT CCTCCTCCAC CAACCCTACC ATTTGGGTTA
    AAACCAAAGA AAGAATTTAA ACCTGAAACC AGCATGAGAA GATTGAATTG GTTAAAGATC
    AGACCTCACG AAATGACTGA AAGCTGTTTC TGGATCAAAG TAAATGAAAA TAAGTATGAA
    AATGTGGATT TGCTCTGTAA ACTTGAGAAT ACGTTTTGTT GCCAACAAAA AGAGAGAAGA
    GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT ATTAAGAAAA AAATTAAAGA GCTTAAATTT
    TTAGATTCTA AAATTGCCCA GAATCTTTCC ATCTTCCTGA GCTCTTTTCG GGTGCCATAT
    GAGGAAATCA AAACGATGAT ATTGGAAGTG GATGAAACAC AGTTGGCAGA GTCTATGATT
    CAGAACCTAA TAAAGCATCT TCCTGATCAA GAACAATTAA GCTCACTGTC TCAGTTCAAG
    AATGACTATA ACAACCTATG TGAACCCGAG CAGTTTGCTG TTGTGATGAG CAATGTGAAG
    AGACTACGGC CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC
    AACATCAAAC CTGACATCAT GGCTGTCAGT ACTGCCTGCG AGGAGATAAA GAAGAGCAAA
    AGCTTCAGCA AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC
    CGGAATGCTC AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACGAAA
    TCAGCAGATC AGAAAACAAC ACTACTTCAT TTTCTGGTGG AAGTATGTGA AGAAAAGTAT
    CCTGATATCC TGAATTTTGT GGATGATTTG GAACATTTAG ACAAAGCTAG TAAAGTCTCT
    GTAGAAACGC TGGAAAAGAA TTTGAAGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAA
    GATTTGGAAA CCTTTCCCCC TCCTGAGGAC TTGCATGATA AGTTTGTGAC AAAGATGTCC
    AGCTTTGTTA TCAGCGCAAA AGAACAATAT GAGAAACTTC TAAAGTTACA TGAAAATATG
    GAGAAGTTAT ACCAGAGTGT GATGGGATAC TATGCCATTG ACGTGAAGAA GATGTCCGTG
    GAAGACTTTT TTAATGACTT GAATAATTTC AGAACCACAT TCATGCAAGC AAGAAAGGAG
    AACATCAAAA AAAGAGAAGC AGAGGAAAGG GAAAAACGTG CCCAAATAGC CAAAGAATTG
    GCAGAGAAAG AAAGACTTGA ACGCCAACAA AAGAAAAAGC GCTTGTTAGA AATGAAGACT
    GAGGGTGATG AGACAGGAGT GATGGATAGT CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC
    TTCCGCGACC GAAGAAAAAG GACGCCAAAG CCAAAAGATA TTCGGCAAAG TCTCAGTCCC
    ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT AACCATGAAA ATCAGAAAGT GCAGTTGACA
    GAAGGGTCAC GTTCACACTA CAATATCAAT TGCAACTCAA CGAGGACTCC AGTCGCCAAG
    GAGCTTAATT ATAATCTAGA CACTCATACG TCTACAGGGA GGATCAAGGC AGTTGAGAAG
    AAGGAAGCCT GTAATGTAGA AAGCAACAGA AAAAAGGAAA TGGAACTTCT TGGCTCGGTT
    TCTAAAAACG AATCTGTTCC CGAAGTTGAA GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23597
    Clone ID Related Accession (Same CDS sequence) XM_005633950.2 , XM_005633951.2 , XM_022408011.1 , XM_005633951.3
    Accession Version XM_005633950.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein diaphanous homolog 3 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTTTCAA GTTCAAGGAA CAGTTGGGTA GAAAGCTTTG GACGCGAGGG GCTTGGATTA 
    TTACTGGACA TTTTGGAAAG ACTGATTAGT GGCAAAATAC AAGAGAAAAT TGTAAAGAAG
    AATCAGCATA AAGTCATACA GTGTCTAAAG GCTTTGATGA ATACACAGTA TGGCTTGGAA
    AGAATTATGA GTGAAGAGAG GAGTCTTTCT CTATTAGCCA AAGCCATCGA TCCTGAGCAC
    CCAAGTATGA TGACAGATGT GGTGAAACTC CTCTCTGCGG TATGCATTGT AGGAGAGGAA
    AGCATTCTCG AAGAGGTTTT AGAAGCTTTA ACATCAGCTG GAGAAGAAAG AAAAATCGAC
    AGATTTTCAT CCATTGTGGA AGGTCTCCGG CATGATTCAG TTCAACTGCA AGTAGCGTGT
    ATGCAGCTCA TCAATGCCCT TGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACATC
    AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATCTTGC CAGATTTAAA ATGTATGAAG
    AATGATAGCC TGGATATCCA ACTTAAAGTC TTTGATGAGC ATAAGGAAGA AGATTTGCTT
    GAGTTCTCCC ATCGCCTTGA AGATATTAGA GCTGAACTTG ATGAAGCATA TGATATTTAC
    AACATGGTGT GGAACTCAGT TAAGGAAACT AAAGCAGAGG GATATTTCAT TTCTATTCTT
    CAGCACCTTT TGCTGATTCG AAATGATTAT AGTATAAGGC AACAGTACTT CAAATTAATT
    GATGAGTGTA TATCTCAGAT TGTATTGCAT AGAGATGGGA TGGATCCAGA CTTCACATAT
    CGGAAAAGAC TGGATTTAGA TTTAAGTCAG TTTGTTGATA CTTGCATAGA TCAAGCAAAA
    CTAGAAGAGT TTGAAGAGAA AGCGTCAGAA CTGCACAAGA AATTTGAAAA AGAGTTTACC
    GACCACCAAG AAACTCAGGC TCAATTGCAG AAAAAAGAAG CAAAGATTAA TGAACTTCAA
    GCAGAGTTAC AAGCTTTTAA GTCAGAGTTT GGTGCCTTGC CAGCTGGTAG AAATCACTCT
    TTGCCACAGT CAAAAGAAAA TGGAACTGGC CCCCCAGCAC TTGCTCCTCC CCTGCCACTG
    CCATCTTGTG GAGGCATGCC GCCTCCACCC CCTCCTCCCC CACCTCCTCC GCTTCCAGGA
    ATGCCAATGC CTTTTGGTGG TCCCGTGCCT CCTCCACCAC CTCCTCTGGG ATTTCTCAGT
    GGGCGAAACT CTCCTCCTCC ACCAACCCTA CCATTTGGGT TAAAACCAAA GAAAGAATTT
    AAACCTGAAA CCAGCATGAG AAGATTGAAT TGGTTAAAGA TCAGACCTCA CGAAATGACT
    GAAAGCTGTT TCTGGATCAA AGTAAATGAA AATAAGTATG AAAATGTGGA TTTGCTCTGT
    AAACTTGAGA ATACGTTTTG TTGCCAACAA AAAGAGAGAA GAGAAGAAGA AGATTTTGAA
    GAAAAGAAAG CTATTAAGAA AAAAATTAAA GAGCTTAAAT TTTTAGATTC TAAAATTGCC
    CAGAATCTTT CCATCTTCCT GAGCTCTTTT CGGGTGCCAT ATGAGGAAAT CAAAACGATG
    ATATTGGAAG TGGATGAAAC ACAGTTGGCA GAGTCTATGA TTCAGAACCT AATAAAGCAT
    CTTCCTGATC AAGAACAATT AAGCTCACTG TCTCAGTTCA AGAATGACTA TAACAACCTA
    TGTGAACCCG AGCAGTTTGC TGTTGTGATG AGCAATGTGA AGAGACTACG GCCACGGCTC
    AGTGCTATTC TCTTTAAGCT TCAGTTTGAA GAGCAGGTGA ACAACATCAA ACCTGACATC
    ATGGCTGTCA GTACTGCCTG CGAGGAGATA AAGAAGAGCA AAAGCTTCAG CAAGTTGCTG
    GAACTTGTAT TGCTAATGGG AAACTACATG AATGCTGGCT CCCGGAATGC TCAAACCTTC
    GGATTTAACC TTAGCTCTCT CTGTAAACTA AAGGACACGA AATCAGCAGA TCAGAAAACA
    ACACTACTTC ATTTTCTGGT GGAAGTATGT GAAGAAAAGT ATCCTGATAT CCTGAATTTT
    GTGGATGATT TGGAACATTT AGACAAAGCT AGTAAAGTCT CTGTAGAAAC GCTGGAAAAG
    AATTTGAAGC AGATGGGAAG GCAGCTTCAA CAGCTTGAGA AAGATTTGGA AACCTTTCCC
    CCTCCTGAGG ACTTGCATGA TAAGTTTGTG ACAAAGATGT CCAGCTTTGT TATCAGCGCA
    AAAGAACAAT ATGAGAAACT TCTAAAGTTA CATGAAAATA TGGAGAAGTT ATACCAGAGT
    GTGATGGGAT ACTATGCCAT TGACGTGAAG AAGATGTCCG TGGAAGACTT TTTTAATGAC
    TTGAATAATT TCAGAACCAC ATTCATGCAA GCAAGAAAGG AGAACATCAA AAAAAGAGAA
    GCAGAGGAAA GGGAAAAACG TGCCCAAATA GCCAAAGAAT TGGCAGAGAA AGAAAGACTT
    GAACGCCAAC AAAAGAAAAA GCGCTTGTTA GAAATGAAGA CTGAGGGTGA TGAGACAGGA
    GTGATGGATA GTCTGCTGGA GGCTTTGCAG TCGGGCGCTG CCTTCCGCGA CCGAAGAAAA
    AGGACGCCAA AGCCAAAAGA TATTCGGCAA AGTCTCAGTC CCATGTCCCA GAGGCCTGTT
    CTGAAAGTTT GTAACCATGA AAATCAGAAA GTGCAGTTGA CAGAAGGGTC ACGTTCACAC
    TACAATATCA ATTGCAACTC AACGAGGACT CCAGTCGCCA AGGAGCTTAA TTATAATCTA
    GACACTCATA CGTCTACAGG GAGGATCAAG GCAGTTGAGA AGAAGGAAGC CTGTAATGTA
    GAAAGCAACA GAAAAAAGGA AATGGAACTT CTTGGCTCGG TTTCTAAAAA CGAATCTGTT
    CCCGAAGTTG AAGCCCTGCT GGCAAGATTA CGAGCTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634007.1
    CDS263..3244
    Translation

    Target ORF information:

    RefSeq Version XM_005633950.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633950.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTTTCAA GTTCAAGGAA CAGTTGGGTA GAAAGCTTTG GACGCGAGGG GCTTGGATTA 
    TTACTGGACA TTTTGGAAAG ACTGATTAGT GGCAAAATAC AAGAGAAAAT TGTAAAGAAG
    AATCAGCATA AAGTCATACA GTGTCTAAAG GCTTTGATGA ATACACAGTA TGGCTTGGAA
    AGAATTATGA GTGAAGAGAG GAGTCTTTCT CTATTAGCCA AAGCCATCGA TCCTGAGCAC
    CCAAGTATGA TGACAGATGT GGTGAAACTC CTCTCTGCGG TATGCATTGT AGGAGAGGAA
    AGCATTCTCG AAGAGGTTTT AGAAGCTTTA ACATCAGCTG GAGAAGAAAG AAAAATCGAC
    AGATTTTCAT CCATTGTGGA AGGTCTCCGG CATGATTCAG TTCAACTGCA AGTAGCGTGT
    ATGCAGCTCA TCAATGCCCT TGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACATC
    AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATCTTGC CAGATTTAAA ATGTATGAAG
    AATGATAGCC TGGATATCCA ACTTAAAGTC TTTGATGAGC ATAAGGAAGA AGATTTGCTT
    GAGTTCTCCC ATCGCCTTGA AGATATTAGA GCTGAACTTG ATGAAGCATA TGATATTTAC
    AACATGGTGT GGAACTCAGT TAAGGAAACT AAAGCAGAGG GATATTTCAT TTCTATTCTT
    CAGCACCTTT TGCTGATTCG AAATGATTAT AGTATAAGGC AACAGTACTT CAAATTAATT
    GATGAGTGTA TATCTCAGAT TGTATTGCAT AGAGATGGGA TGGATCCAGA CTTCACATAT
    CGGAAAAGAC TGGATTTAGA TTTAAGTCAG TTTGTTGATA CTTGCATAGA TCAAGCAAAA
    CTAGAAGAGT TTGAAGAGAA AGCGTCAGAA CTGCACAAGA AATTTGAAAA AGAGTTTACC
    GACCACCAAG AAACTCAGGC TCAATTGCAG AAAAAAGAAG CAAAGATTAA TGAACTTCAA
    GCAGAGTTAC AAGCTTTTAA GTCAGAGTTT GGTGCCTTGC CAGCTGGTAG AAATCACTCT
    TTGCCACAGT CAAAAGAAAA TGGAACTGGC CCCCCAGCAC TTGCTCCTCC CCTGCCACTG
    CCATCTTGTG GAGGCATGCC GCCTCCACCC CCTCCTCCCC CACCTCCTCC GCTTCCAGGA
    ATGCCAATGC CTTTTGGTGG TCCCGTGCCT CCTCCACCAC CTCCTCTGGG ATTTCTCAGT
    GGGCGAAACT CTCCTCCTCC ACCAACCCTA CCATTTGGGT TAAAACCAAA GAAAGAATTT
    AAACCTGAAA CCAGCATGAG AAGATTGAAT TGGTTAAAGA TCAGACCTCA CGAAATGACT
    GAAAGCTGTT TCTGGATCAA AGTAAATGAA AATAAGTATG AAAATGTGGA TTTGCTCTGT
    AAACTTGAGA ATACGTTTTG TTGCCAACAA AAAGAGAGAA GAGAAGAAGA AGATTTTGAA
    GAAAAGAAAG CTATTAAGAA AAAAATTAAA GAGCTTAAAT TTTTAGATTC TAAAATTGCC
    CAGAATCTTT CCATCTTCCT GAGCTCTTTT CGGGTGCCAT ATGAGGAAAT CAAAACGATG
    ATATTGGAAG TGGATGAAAC ACAGTTGGCA GAGTCTATGA TTCAGAACCT AATAAAGCAT
    CTTCCTGATC AAGAACAATT AAGCTCACTG TCTCAGTTCA AGAATGACTA TAACAACCTA
    TGTGAACCCG AGCAGTTTGC TGTTGTGATG AGCAATGTGA AGAGACTACG GCCACGGCTC
    AGTGCTATTC TCTTTAAGCT TCAGTTTGAA GAGCAGGTGA ACAACATCAA ACCTGACATC
    ATGGCTGTCA GTACTGCCTG CGAGGAGATA AAGAAGAGCA AAAGCTTCAG CAAGTTGCTG
    GAACTTGTAT TGCTAATGGG AAACTACATG AATGCTGGCT CCCGGAATGC TCAAACCTTC
    GGATTTAACC TTAGCTCTCT CTGTAAACTA AAGGACACGA AATCAGCAGA TCAGAAAACA
    ACACTACTTC ATTTTCTGGT GGAAGTATGT GAAGAAAAGT ATCCTGATAT CCTGAATTTT
    GTGGATGATT TGGAACATTT AGACAAAGCT AGTAAAGTCT CTGTAGAAAC GCTGGAAAAG
    AATTTGAAGC AGATGGGAAG GCAGCTTCAA CAGCTTGAGA AAGATTTGGA AACCTTTCCC
    CCTCCTGAGG ACTTGCATGA TAAGTTTGTG ACAAAGATGT CCAGCTTTGT TATCAGCGCA
    AAAGAACAAT ATGAGAAACT TCTAAAGTTA CATGAAAATA TGGAGAAGTT ATACCAGAGT
    GTGATGGGAT ACTATGCCAT TGACGTGAAG AAGATGTCCG TGGAAGACTT TTTTAATGAC
    TTGAATAATT TCAGAACCAC ATTCATGCAA GCAAGAAAGG AGAACATCAA AAAAAGAGAA
    GCAGAGGAAA GGGAAAAACG TGCCCAAATA GCCAAAGAAT TGGCAGAGAA AGAAAGACTT
    GAACGCCAAC AAAAGAAAAA GCGCTTGTTA GAAATGAAGA CTGAGGGTGA TGAGACAGGA
    GTGATGGATA GTCTGCTGGA GGCTTTGCAG TCGGGCGCTG CCTTCCGCGA CCGAAGAAAA
    AGGACGCCAA AGCCAAAAGA TATTCGGCAA AGTCTCAGTC CCATGTCCCA GAGGCCTGTT
    CTGAAAGTTT GTAACCATGA AAATCAGAAA GTGCAGTTGA CAGAAGGGTC ACGTTCACAC
    TACAATATCA ATTGCAACTC AACGAGGACT CCAGTCGCCA AGGAGCTTAA TTATAATCTA
    GACACTCATA CGTCTACAGG GAGGATCAAG GCAGTTGAGA AGAAGGAAGC CTGTAATGTA
    GAAAGCAACA GAAAAAAGGA AATGGAACTT CTTGGCTCGG TTTCTAAAAA CGAATCTGTT
    CCCGAAGTTG AAGCCCTGCT GGCAAGATTA CGAGCTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23597
    Clone ID Related Accession (Same CDS sequence) XM_005633950.2 , XM_005633951.2 , XM_022408011.1 , XM_005633951.3
    Accession Version XM_005633951.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein diaphanous homolog 3 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTTTCAA GTTCAAGGAA CAGTTGGGTA GAAAGCTTTG GACGCGAGGG GCTTGGATTA 
    TTACTGGACA TTTTGGAAAG ACTGATTAGT GGCAAAATAC AAGAGAAAAT TGTAAAGAAG
    AATCAGCATA AAGTCATACA GTGTCTAAAG GCTTTGATGA ATACACAGTA TGGCTTGGAA
    AGAATTATGA GTGAAGAGAG GAGTCTTTCT CTATTAGCCA AAGCCATCGA TCCTGAGCAC
    CCAAGTATGA TGACAGATGT GGTGAAACTC CTCTCTGCGG TATGCATTGT AGGAGAGGAA
    AGCATTCTCG AAGAGGTTTT AGAAGCTTTA ACATCAGCTG GAGAAGAAAG AAAAATCGAC
    AGATTTTCAT CCATTGTGGA AGGTCTCCGG CATGATTCAG TTCAACTGCA AGTAGCGTGT
    ATGCAGCTCA TCAATGCCCT TGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACATC
    AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATCTTGC CAGATTTAAA ATGTATGAAG
    AATGATAGCC TGGATATCCA ACTTAAAGTC TTTGATGAGC ATAAGGAAGA AGATTTGCTT
    GAGTTCTCCC ATCGCCTTGA AGATATTAGA GCTGAACTTG ATGAAGCATA TGATATTTAC
    AACATGGTGT GGAACTCAGT TAAGGAAACT AAAGCAGAGG GATATTTCAT TTCTATTCTT
    CAGCACCTTT TGCTGATTCG AAATGATTAT AGTATAAGGC AACAGTACTT CAAATTAATT
    GATGAGTGTA TATCTCAGAT TGTATTGCAT AGAGATGGGA TGGATCCAGA CTTCACATAT
    CGGAAAAGAC TGGATTTAGA TTTAAGTCAG TTTGTTGATA CTTGCATAGA TCAAGCAAAA
    CTAGAAGAGT TTGAAGAGAA AGCGTCAGAA CTGCACAAGA AATTTGAAAA AGAGTTTACC
    GACCACCAAG AAACTCAGGC TCAATTGCAG AAAAAAGAAG CAAAGATTAA TGAACTTCAA
    GCAGAGTTAC AAGCTTTTAA GTCAGAGTTT GGTGCCTTGC CAGCTGGTAG AAATCACTCT
    TTGCCACAGT CAAAAGAAAA TGGAACTGGC CCCCCAGCAC TTGCTCCTCC CCTGCCACTG
    CCATCTTGTG GAGGCATGCC GCCTCCACCC CCTCCTCCCC CACCTCCTCC GCTTCCAGGA
    ATGCCAATGC CTTTTGGTGG TCCCGTGCCT CCTCCACCAC CTCCTCTGGG ATTTCTCAGT
    GGGCGAAACT CTCCTCCTCC ACCAACCCTA CCATTTGGGT TAAAACCAAA GAAAGAATTT
    AAACCTGAAA CCAGCATGAG AAGATTGAAT TGGTTAAAGA TCAGACCTCA CGAAATGACT
    GAAAGCTGTT TCTGGATCAA AGTAAATGAA AATAAGTATG AAAATGTGGA TTTGCTCTGT
    AAACTTGAGA ATACGTTTTG TTGCCAACAA AAAGAGAGAA GAGAAGAAGA AGATTTTGAA
    GAAAAGAAAG CTATTAAGAA AAAAATTAAA GAGCTTAAAT TTTTAGATTC TAAAATTGCC
    CAGAATCTTT CCATCTTCCT GAGCTCTTTT CGGGTGCCAT ATGAGGAAAT CAAAACGATG
    ATATTGGAAG TGGATGAAAC ACAGTTGGCA GAGTCTATGA TTCAGAACCT AATAAAGCAT
    CTTCCTGATC AAGAACAATT AAGCTCACTG TCTCAGTTCA AGAATGACTA TAACAACCTA
    TGTGAACCCG AGCAGTTTGC TGTTGTGATG AGCAATGTGA AGAGACTACG GCCACGGCTC
    AGTGCTATTC TCTTTAAGCT TCAGTTTGAA GAGCAGGTGA ACAACATCAA ACCTGACATC
    ATGGCTGTCA GTACTGCCTG CGAGGAGATA AAGAAGAGCA AAAGCTTCAG CAAGTTGCTG
    GAACTTGTAT TGCTAATGGG AAACTACATG AATGCTGGCT CCCGGAATGC TCAAACCTTC
    GGATTTAACC TTAGCTCTCT CTGTAAACTA AAGGACACGA AATCAGCAGA TCAGAAAACA
    ACACTACTTC ATTTTCTGGT GGAAGTATGT GAAGAAAAGT ATCCTGATAT CCTGAATTTT
    GTGGATGATT TGGAACATTT AGACAAAGCT AGTAAAGTCT CTGTAGAAAC GCTGGAAAAG
    AATTTGAAGC AGATGGGAAG GCAGCTTCAA CAGCTTGAGA AAGATTTGGA AACCTTTCCC
    CCTCCTGAGG ACTTGCATGA TAAGTTTGTG ACAAAGATGT CCAGCTTTGT TATCAGCGCA
    AAAGAACAAT ATGAGAAACT TCTAAAGTTA CATGAAAATA TGGAGAAGTT ATACCAGAGT
    GTGATGGGAT ACTATGCCAT TGACGTGAAG AAGATGTCCG TGGAAGACTT TTTTAATGAC
    TTGAATAATT TCAGAACCAC ATTCATGCAA GCAAGAAAGG AGAACATCAA AAAAAGAGAA
    GCAGAGGAAA GGGAAAAACG TGCCCAAATA GCCAAAGAAT TGGCAGAGAA AGAAAGACTT
    GAACGCCAAC AAAAGAAAAA GCGCTTGTTA GAAATGAAGA CTGAGGGTGA TGAGACAGGA
    GTGATGGATA GTCTGCTGGA GGCTTTGCAG TCGGGCGCTG CCTTCCGCGA CCGAAGAAAA
    AGGACGCCAA AGCCAAAAGA TATTCGGCAA AGTCTCAGTC CCATGTCCCA GAGGCCTGTT
    CTGAAAGTTT GTAACCATGA AAATCAGAAA GTGCAGTTGA CAGAAGGGTC ACGTTCACAC
    TACAATATCA ATTGCAACTC AACGAGGACT CCAGTCGCCA AGGAGCTTAA TTATAATCTA
    GACACTCATA CGTCTACAGG GAGGATCAAG GCAGTTGAGA AGAAGGAAGC CTGTAATGTA
    GAAAGCAACA GAAAAAAGGA AATGGAACTT CTTGGCTCGG TTTCTAAAAA CGAATCTGTT
    CCCGAAGTTG AAGCCCTGCT GGCAAGATTA CGAGCTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634008.1
    CDS81..3062
    Translation

    Target ORF information:

    RefSeq Version XM_005633951.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633951.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTTTCAA GTTCAAGGAA CAGTTGGGTA GAAAGCTTTG GACGCGAGGG GCTTGGATTA 
    TTACTGGACA TTTTGGAAAG ACTGATTAGT GGCAAAATAC AAGAGAAAAT TGTAAAGAAG
    AATCAGCATA AAGTCATACA GTGTCTAAAG GCTTTGATGA ATACACAGTA TGGCTTGGAA
    AGAATTATGA GTGAAGAGAG GAGTCTTTCT CTATTAGCCA AAGCCATCGA TCCTGAGCAC
    CCAAGTATGA TGACAGATGT GGTGAAACTC CTCTCTGCGG TATGCATTGT AGGAGAGGAA
    AGCATTCTCG AAGAGGTTTT AGAAGCTTTA ACATCAGCTG GAGAAGAAAG AAAAATCGAC
    AGATTTTCAT CCATTGTGGA AGGTCTCCGG CATGATTCAG TTCAACTGCA AGTAGCGTGT
    ATGCAGCTCA TCAATGCCCT TGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACATC
    AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATCTTGC CAGATTTAAA ATGTATGAAG
    AATGATAGCC TGGATATCCA ACTTAAAGTC TTTGATGAGC ATAAGGAAGA AGATTTGCTT
    GAGTTCTCCC ATCGCCTTGA AGATATTAGA GCTGAACTTG ATGAAGCATA TGATATTTAC
    AACATGGTGT GGAACTCAGT TAAGGAAACT AAAGCAGAGG GATATTTCAT TTCTATTCTT
    CAGCACCTTT TGCTGATTCG AAATGATTAT AGTATAAGGC AACAGTACTT CAAATTAATT
    GATGAGTGTA TATCTCAGAT TGTATTGCAT AGAGATGGGA TGGATCCAGA CTTCACATAT
    CGGAAAAGAC TGGATTTAGA TTTAAGTCAG TTTGTTGATA CTTGCATAGA TCAAGCAAAA
    CTAGAAGAGT TTGAAGAGAA AGCGTCAGAA CTGCACAAGA AATTTGAAAA AGAGTTTACC
    GACCACCAAG AAACTCAGGC TCAATTGCAG AAAAAAGAAG CAAAGATTAA TGAACTTCAA
    GCAGAGTTAC AAGCTTTTAA GTCAGAGTTT GGTGCCTTGC CAGCTGGTAG AAATCACTCT
    TTGCCACAGT CAAAAGAAAA TGGAACTGGC CCCCCAGCAC TTGCTCCTCC CCTGCCACTG
    CCATCTTGTG GAGGCATGCC GCCTCCACCC CCTCCTCCCC CACCTCCTCC GCTTCCAGGA
    ATGCCAATGC CTTTTGGTGG TCCCGTGCCT CCTCCACCAC CTCCTCTGGG ATTTCTCAGT
    GGGCGAAACT CTCCTCCTCC ACCAACCCTA CCATTTGGGT TAAAACCAAA GAAAGAATTT
    AAACCTGAAA CCAGCATGAG AAGATTGAAT TGGTTAAAGA TCAGACCTCA CGAAATGACT
    GAAAGCTGTT TCTGGATCAA AGTAAATGAA AATAAGTATG AAAATGTGGA TTTGCTCTGT
    AAACTTGAGA ATACGTTTTG TTGCCAACAA AAAGAGAGAA GAGAAGAAGA AGATTTTGAA
    GAAAAGAAAG CTATTAAGAA AAAAATTAAA GAGCTTAAAT TTTTAGATTC TAAAATTGCC
    CAGAATCTTT CCATCTTCCT GAGCTCTTTT CGGGTGCCAT ATGAGGAAAT CAAAACGATG
    ATATTGGAAG TGGATGAAAC ACAGTTGGCA GAGTCTATGA TTCAGAACCT AATAAAGCAT
    CTTCCTGATC AAGAACAATT AAGCTCACTG TCTCAGTTCA AGAATGACTA TAACAACCTA
    TGTGAACCCG AGCAGTTTGC TGTTGTGATG AGCAATGTGA AGAGACTACG GCCACGGCTC
    AGTGCTATTC TCTTTAAGCT TCAGTTTGAA GAGCAGGTGA ACAACATCAA ACCTGACATC
    ATGGCTGTCA GTACTGCCTG CGAGGAGATA AAGAAGAGCA AAAGCTTCAG CAAGTTGCTG
    GAACTTGTAT TGCTAATGGG AAACTACATG AATGCTGGCT CCCGGAATGC TCAAACCTTC
    GGATTTAACC TTAGCTCTCT CTGTAAACTA AAGGACACGA AATCAGCAGA TCAGAAAACA
    ACACTACTTC ATTTTCTGGT GGAAGTATGT GAAGAAAAGT ATCCTGATAT CCTGAATTTT
    GTGGATGATT TGGAACATTT AGACAAAGCT AGTAAAGTCT CTGTAGAAAC GCTGGAAAAG
    AATTTGAAGC AGATGGGAAG GCAGCTTCAA CAGCTTGAGA AAGATTTGGA AACCTTTCCC
    CCTCCTGAGG ACTTGCATGA TAAGTTTGTG ACAAAGATGT CCAGCTTTGT TATCAGCGCA
    AAAGAACAAT ATGAGAAACT TCTAAAGTTA CATGAAAATA TGGAGAAGTT ATACCAGAGT
    GTGATGGGAT ACTATGCCAT TGACGTGAAG AAGATGTCCG TGGAAGACTT TTTTAATGAC
    TTGAATAATT TCAGAACCAC ATTCATGCAA GCAAGAAAGG AGAACATCAA AAAAAGAGAA
    GCAGAGGAAA GGGAAAAACG TGCCCAAATA GCCAAAGAAT TGGCAGAGAA AGAAAGACTT
    GAACGCCAAC AAAAGAAAAA GCGCTTGTTA GAAATGAAGA CTGAGGGTGA TGAGACAGGA
    GTGATGGATA GTCTGCTGGA GGCTTTGCAG TCGGGCGCTG CCTTCCGCGA CCGAAGAAAA
    AGGACGCCAA AGCCAAAAGA TATTCGGCAA AGTCTCAGTC CCATGTCCCA GAGGCCTGTT
    CTGAAAGTTT GTAACCATGA AAATCAGAAA GTGCAGTTGA CAGAAGGGTC ACGTTCACAC
    TACAATATCA ATTGCAACTC AACGAGGACT CCAGTCGCCA AGGAGCTTAA TTATAATCTA
    GACACTCATA CGTCTACAGG GAGGATCAAG GCAGTTGAGA AGAAGGAAGC CTGTAATGTA
    GAAAGCAACA GAAAAAAGGA AATGGAACTT CTTGGCTCGG TTTCTAAAAA CGAATCTGTT
    CCCGAAGTTG AAGCCCTGCT GGCAAGATTA CGAGCTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23597
    Clone ID Related Accession (Same CDS sequence) XM_005633950.2 , XM_005633951.2 , XM_022408011.1 , XM_005633951.3
    Accession Version XM_022408011.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTTTCAA GTTCAAGGAA CAGTTGGGTA GAAAGCTTTG GACGCGAGGG GCTTGGATTA 
    TTACTGGACA TTTTGGAAAG ACTGATTAGT GGCAAAATAC AAGAGAAAAT TGTAAAGAAG
    AATCAGCATA AAGTCATACA GTGTCTAAAG GCTTTGATGA ATACACAGTA TGGCTTGGAA
    AGAATTATGA GTGAAGAGAG GAGTCTTTCT CTATTAGCCA AAGCCATCGA TCCTGAGCAC
    CCAAGTATGA TGACAGATGT GGTGAAACTC CTCTCTGCGG TATGCATTGT AGGAGAGGAA
    AGCATTCTCG AAGAGGTTTT AGAAGCTTTA ACATCAGCTG GAGAAGAAAG AAAAATCGAC
    AGATTTTCAT CCATTGTGGA AGGTCTCCGG CATGATTCAG TTCAACTGCA AGTAGCGTGT
    ATGCAGCTCA TCAATGCCCT TGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACATC
    AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATCTTGC CAGATTTAAA ATGTATGAAG
    AATGATAGCC TGGATATCCA ACTTAAAGTC TTTGATGAGC ATAAGGAAGA AGATTTGCTT
    GAGTTCTCCC ATCGCCTTGA AGATATTAGA GCTGAACTTG ATGAAGCATA TGATATTTAC
    AACATGGTGT GGAACTCAGT TAAGGAAACT AAAGCAGAGG GATATTTCAT TTCTATTCTT
    CAGCACCTTT TGCTGATTCG AAATGATTAT AGTATAAGGC AACAGTACTT CAAATTAATT
    GATGAGTGTA TATCTCAGAT TGTATTGCAT AGAGATGGGA TGGATCCAGA CTTCACATAT
    CGGAAAAGAC TGGATTTAGA TTTAAGTCAG TTTGTTGATA CTTGCATAGA TCAAGCAAAA
    CTAGAAGAGT TTGAAGAGAA AGCGTCAGAA CTGCACAAGA AATTTGAAAA AGAGTTTACC
    GACCACCAAG AAACTCAGGC TCAATTGCAG AAAAAAGAAG CAAAGATTAA TGAACTTCAA
    GCAGAGTTAC AAGCTTTTAA GTCAGAGTTT GGTGCCTTGC CAGCTGGTAG AAATCACTCT
    TTGCCACAGT CAAAAGAAAA TGGAACTGGC CCCCCAGCAC TTGCTCCTCC CCTGCCACTG
    CCATCTTGTG GAGGCATGCC GCCTCCACCC CCTCCTCCCC CACCTCCTCC GCTTCCAGGA
    ATGCCAATGC CTTTTGGTGG TCCCGTGCCT CCTCCACCAC CTCCTCTGGG ATTTCTCAGT
    GGGCGAAACT CTCCTCCTCC ACCAACCCTA CCATTTGGGT TAAAACCAAA GAAAGAATTT
    AAACCTGAAA CCAGCATGAG AAGATTGAAT TGGTTAAAGA TCAGACCTCA CGAAATGACT
    GAAAGCTGTT TCTGGATCAA AGTAAATGAA AATAAGTATG AAAATGTGGA TTTGCTCTGT
    AAACTTGAGA ATACGTTTTG TTGCCAACAA AAAGAGAGAA GAGAAGAAGA AGATTTTGAA
    GAAAAGAAAG CTATTAAGAA AAAAATTAAA GAGCTTAAAT TTTTAGATTC TAAAATTGCC
    CAGAATCTTT CCATCTTCCT GAGCTCTTTT CGGGTGCCAT ATGAGGAAAT CAAAACGATG
    ATATTGGAAG TGGATGAAAC ACAGTTGGCA GAGTCTATGA TTCAGAACCT AATAAAGCAT
    CTTCCTGATC AAGAACAATT AAGCTCACTG TCTCAGTTCA AGAATGACTA TAACAACCTA
    TGTGAACCCG AGCAGTTTGC TGTTGTGATG AGCAATGTGA AGAGACTACG GCCACGGCTC
    AGTGCTATTC TCTTTAAGCT TCAGTTTGAA GAGCAGGTGA ACAACATCAA ACCTGACATC
    ATGGCTGTCA GTACTGCCTG CGAGGAGATA AAGAAGAGCA AAAGCTTCAG CAAGTTGCTG
    GAACTTGTAT TGCTAATGGG AAACTACATG AATGCTGGCT CCCGGAATGC TCAAACCTTC
    GGATTTAACC TTAGCTCTCT CTGTAAACTA AAGGACACGA AATCAGCAGA TCAGAAAACA
    ACACTACTTC ATTTTCTGGT GGAAGTATGT GAAGAAAAGT ATCCTGATAT CCTGAATTTT
    GTGGATGATT TGGAACATTT AGACAAAGCT AGTAAAGTCT CTGTAGAAAC GCTGGAAAAG
    AATTTGAAGC AGATGGGAAG GCAGCTTCAA CAGCTTGAGA AAGATTTGGA AACCTTTCCC
    CCTCCTGAGG ACTTGCATGA TAAGTTTGTG ACAAAGATGT CCAGCTTTGT TATCAGCGCA
    AAAGAACAAT ATGAGAAACT TCTAAAGTTA CATGAAAATA TGGAGAAGTT ATACCAGAGT
    GTGATGGGAT ACTATGCCAT TGACGTGAAG AAGATGTCCG TGGAAGACTT TTTTAATGAC
    TTGAATAATT TCAGAACCAC ATTCATGCAA GCAAGAAAGG AGAACATCAA AAAAAGAGAA
    GCAGAGGAAA GGGAAAAACG TGCCCAAATA GCCAAAGAAT TGGCAGAGAA AGAAAGACTT
    GAACGCCAAC AAAAGAAAAA GCGCTTGTTA GAAATGAAGA CTGAGGGTGA TGAGACAGGA
    GTGATGGATA GTCTGCTGGA GGCTTTGCAG TCGGGCGCTG CCTTCCGCGA CCGAAGAAAA
    AGGACGCCAA AGCCAAAAGA TATTCGGCAA AGTCTCAGTC CCATGTCCCA GAGGCCTGTT
    CTGAAAGTTT GTAACCATGA AAATCAGAAA GTGCAGTTGA CAGAAGGGTC ACGTTCACAC
    TACAATATCA ATTGCAACTC AACGAGGACT CCAGTCGCCA AGGAGCTTAA TTATAATCTA
    GACACTCATA CGTCTACAGG GAGGATCAAG GCAGTTGAGA AGAAGGAAGC CTGTAATGTA
    GAAAGCAACA GAAAAAAGGA AATGGAACTT CTTGGCTCGG TTTCTAAAAA CGAATCTGTT
    CCCGAAGTTG AAGCCCTGCT GGCAAGATTA CGAGCTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022263719.1
    CDS280..3261
    Translation

    Target ORF information:

    RefSeq Version XM_022408011.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022408011.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTTTCAA GTTCAAGGAA CAGTTGGGTA GAAAGCTTTG GACGCGAGGG GCTTGGATTA 
    TTACTGGACA TTTTGGAAAG ACTGATTAGT GGCAAAATAC AAGAGAAAAT TGTAAAGAAG
    AATCAGCATA AAGTCATACA GTGTCTAAAG GCTTTGATGA ATACACAGTA TGGCTTGGAA
    AGAATTATGA GTGAAGAGAG GAGTCTTTCT CTATTAGCCA AAGCCATCGA TCCTGAGCAC
    CCAAGTATGA TGACAGATGT GGTGAAACTC CTCTCTGCGG TATGCATTGT AGGAGAGGAA
    AGCATTCTCG AAGAGGTTTT AGAAGCTTTA ACATCAGCTG GAGAAGAAAG AAAAATCGAC
    AGATTTTCAT CCATTGTGGA AGGTCTCCGG CATGATTCAG TTCAACTGCA AGTAGCGTGT
    ATGCAGCTCA TCAATGCCCT TGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACATC
    AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATCTTGC CAGATTTAAA ATGTATGAAG
    AATGATAGCC TGGATATCCA ACTTAAAGTC TTTGATGAGC ATAAGGAAGA AGATTTGCTT
    GAGTTCTCCC ATCGCCTTGA AGATATTAGA GCTGAACTTG ATGAAGCATA TGATATTTAC
    AACATGGTGT GGAACTCAGT TAAGGAAACT AAAGCAGAGG GATATTTCAT TTCTATTCTT
    CAGCACCTTT TGCTGATTCG AAATGATTAT AGTATAAGGC AACAGTACTT CAAATTAATT
    GATGAGTGTA TATCTCAGAT TGTATTGCAT AGAGATGGGA TGGATCCAGA CTTCACATAT
    CGGAAAAGAC TGGATTTAGA TTTAAGTCAG TTTGTTGATA CTTGCATAGA TCAAGCAAAA
    CTAGAAGAGT TTGAAGAGAA AGCGTCAGAA CTGCACAAGA AATTTGAAAA AGAGTTTACC
    GACCACCAAG AAACTCAGGC TCAATTGCAG AAAAAAGAAG CAAAGATTAA TGAACTTCAA
    GCAGAGTTAC AAGCTTTTAA GTCAGAGTTT GGTGCCTTGC CAGCTGGTAG AAATCACTCT
    TTGCCACAGT CAAAAGAAAA TGGAACTGGC CCCCCAGCAC TTGCTCCTCC CCTGCCACTG
    CCATCTTGTG GAGGCATGCC GCCTCCACCC CCTCCTCCCC CACCTCCTCC GCTTCCAGGA
    ATGCCAATGC CTTTTGGTGG TCCCGTGCCT CCTCCACCAC CTCCTCTGGG ATTTCTCAGT
    GGGCGAAACT CTCCTCCTCC ACCAACCCTA CCATTTGGGT TAAAACCAAA GAAAGAATTT
    AAACCTGAAA CCAGCATGAG AAGATTGAAT TGGTTAAAGA TCAGACCTCA CGAAATGACT
    GAAAGCTGTT TCTGGATCAA AGTAAATGAA AATAAGTATG AAAATGTGGA TTTGCTCTGT
    AAACTTGAGA ATACGTTTTG TTGCCAACAA AAAGAGAGAA GAGAAGAAGA AGATTTTGAA
    GAAAAGAAAG CTATTAAGAA AAAAATTAAA GAGCTTAAAT TTTTAGATTC TAAAATTGCC
    CAGAATCTTT CCATCTTCCT GAGCTCTTTT CGGGTGCCAT ATGAGGAAAT CAAAACGATG
    ATATTGGAAG TGGATGAAAC ACAGTTGGCA GAGTCTATGA TTCAGAACCT AATAAAGCAT
    CTTCCTGATC AAGAACAATT AAGCTCACTG TCTCAGTTCA AGAATGACTA TAACAACCTA
    TGTGAACCCG AGCAGTTTGC TGTTGTGATG AGCAATGTGA AGAGACTACG GCCACGGCTC
    AGTGCTATTC TCTTTAAGCT TCAGTTTGAA GAGCAGGTGA ACAACATCAA ACCTGACATC
    ATGGCTGTCA GTACTGCCTG CGAGGAGATA AAGAAGAGCA AAAGCTTCAG CAAGTTGCTG
    GAACTTGTAT TGCTAATGGG AAACTACATG AATGCTGGCT CCCGGAATGC TCAAACCTTC
    GGATTTAACC TTAGCTCTCT CTGTAAACTA AAGGACACGA AATCAGCAGA TCAGAAAACA
    ACACTACTTC ATTTTCTGGT GGAAGTATGT GAAGAAAAGT ATCCTGATAT CCTGAATTTT
    GTGGATGATT TGGAACATTT AGACAAAGCT AGTAAAGTCT CTGTAGAAAC GCTGGAAAAG
    AATTTGAAGC AGATGGGAAG GCAGCTTCAA CAGCTTGAGA AAGATTTGGA AACCTTTCCC
    CCTCCTGAGG ACTTGCATGA TAAGTTTGTG ACAAAGATGT CCAGCTTTGT TATCAGCGCA
    AAAGAACAAT ATGAGAAACT TCTAAAGTTA CATGAAAATA TGGAGAAGTT ATACCAGAGT
    GTGATGGGAT ACTATGCCAT TGACGTGAAG AAGATGTCCG TGGAAGACTT TTTTAATGAC
    TTGAATAATT TCAGAACCAC ATTCATGCAA GCAAGAAAGG AGAACATCAA AAAAAGAGAA
    GCAGAGGAAA GGGAAAAACG TGCCCAAATA GCCAAAGAAT TGGCAGAGAA AGAAAGACTT
    GAACGCCAAC AAAAGAAAAA GCGCTTGTTA GAAATGAAGA CTGAGGGTGA TGAGACAGGA
    GTGATGGATA GTCTGCTGGA GGCTTTGCAG TCGGGCGCTG CCTTCCGCGA CCGAAGAAAA
    AGGACGCCAA AGCCAAAAGA TATTCGGCAA AGTCTCAGTC CCATGTCCCA GAGGCCTGTT
    CTGAAAGTTT GTAACCATGA AAATCAGAAA GTGCAGTTGA CAGAAGGGTC ACGTTCACAC
    TACAATATCA ATTGCAACTC AACGAGGACT CCAGTCGCCA AGGAGCTTAA TTATAATCTA
    GACACTCATA CGTCTACAGG GAGGATCAAG GCAGTTGAGA AGAAGGAAGC CTGTAATGTA
    GAAAGCAACA GAAAAAAGGA AATGGAACTT CTTGGCTCGG TTTCTAAAAA CGAATCTGTT
    CCCGAAGTTG AAGCCCTGCT GGCAAGATTA CGAGCTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23597
    Clone ID Related Accession (Same CDS sequence) XM_005633950.2 , XM_005633951.2 , XM_022408011.1 , XM_005633951.3
    Accession Version XM_005633951.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTTTCAA GTTCAAGGAA CAGTTGGGTA GAAAGCTTTG GACGCGAGGG GCTTGGATTA 
    TTACTGGACA TTTTGGAAAG ACTGATTAGT GGCAAAATAC AAGAGAAAAT TGTAAAGAAG
    AATCAGCATA AAGTCATACA GTGTCTAAAG GCTTTGATGA ATACACAGTA TGGCTTGGAA
    AGAATTATGA GTGAAGAGAG GAGTCTTTCT CTATTAGCCA AAGCCATCGA TCCTGAGCAC
    CCAAGTATGA TGACAGATGT GGTGAAACTC CTCTCTGCGG TATGCATTGT AGGAGAGGAA
    AGCATTCTCG AAGAGGTTTT AGAAGCTTTA ACATCAGCTG GAGAAGAAAG AAAAATCGAC
    AGATTTTCAT CCATTGTGGA AGGTCTCCGG CATGATTCAG TTCAACTGCA AGTAGCGTGT
    ATGCAGCTCA TCAATGCCCT TGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACATC
    AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATCTTGC CAGATTTAAA ATGTATGAAG
    AATGATAGCC TGGATATCCA ACTTAAAGTC TTTGATGAGC ATAAGGAAGA AGATTTGCTT
    GAGTTCTCCC ATCGCCTTGA AGATATTAGA GCTGAACTTG ATGAAGCATA TGATATTTAC
    AACATGGTGT GGAACTCAGT TAAGGAAACT AAAGCAGAGG GATATTTCAT TTCTATTCTT
    CAGCACCTTT TGCTGATTCG AAATGATTAT AGTATAAGGC AACAGTACTT CAAATTAATT
    GATGAGTGTA TATCTCAGAT TGTATTGCAT AGAGATGGGA TGGATCCAGA CTTCACATAT
    CGGAAAAGAC TGGATTTAGA TTTAAGTCAG TTTGTTGATA CTTGCATAGA TCAAGCAAAA
    CTAGAAGAGT TTGAAGAGAA AGCGTCAGAA CTGCACAAGA AATTTGAAAA AGAGTTTACC
    GACCACCAAG AAACTCAGGC TCAATTGCAG AAAAAAGAAG CAAAGATTAA TGAACTTCAA
    GCAGAGTTAC AAGCTTTTAA GTCAGAGTTT GGTGCCTTGC CAGCTGGTAG AAATCACTCT
    TTGCCACAGT CAAAAGAAAA TGGAACTGGC CCCCCAGCAC TTGCTCCTCC CCTGCCACTG
    CCATCTTGTG GAGGCATGCC GCCTCCACCC CCTCCTCCCC CACCTCCTCC GCTTCCAGGA
    ATGCCAATGC CTTTTGGTGG TCCCGTGCCT CCTCCACCAC CTCCTCTGGG ATTTCTCAGT
    GGGCGAAACT CTCCTCCTCC ACCAACCCTA CCATTTGGGT TAAAACCAAA GAAAGAATTT
    AAACCTGAAA CCAGCATGAG AAGATTGAAT TGGTTAAAGA TCAGACCTCA CGAAATGACT
    GAAAGCTGTT TCTGGATCAA AGTAAATGAA AATAAGTATG AAAATGTGGA TTTGCTCTGT
    AAACTTGAGA ATACGTTTTG TTGCCAACAA AAAGAGAGAA GAGAAGAAGA AGATTTTGAA
    GAAAAGAAAG CTATTAAGAA AAAAATTAAA GAGCTTAAAT TTTTAGATTC TAAAATTGCC
    CAGAATCTTT CCATCTTCCT GAGCTCTTTT CGGGTGCCAT ATGAGGAAAT CAAAACGATG
    ATATTGGAAG TGGATGAAAC ACAGTTGGCA GAGTCTATGA TTCAGAACCT AATAAAGCAT
    CTTCCTGATC AAGAACAATT AAGCTCACTG TCTCAGTTCA AGAATGACTA TAACAACCTA
    TGTGAACCCG AGCAGTTTGC TGTTGTGATG AGCAATGTGA AGAGACTACG GCCACGGCTC
    AGTGCTATTC TCTTTAAGCT TCAGTTTGAA GAGCAGGTGA ACAACATCAA ACCTGACATC
    ATGGCTGTCA GTACTGCCTG CGAGGAGATA AAGAAGAGCA AAAGCTTCAG CAAGTTGCTG
    GAACTTGTAT TGCTAATGGG AAACTACATG AATGCTGGCT CCCGGAATGC TCAAACCTTC
    GGATTTAACC TTAGCTCTCT CTGTAAACTA AAGGACACGA AATCAGCAGA TCAGAAAACA
    ACACTACTTC ATTTTCTGGT GGAAGTATGT GAAGAAAAGT ATCCTGATAT CCTGAATTTT
    GTGGATGATT TGGAACATTT AGACAAAGCT AGTAAAGTCT CTGTAGAAAC GCTGGAAAAG
    AATTTGAAGC AGATGGGAAG GCAGCTTCAA CAGCTTGAGA AAGATTTGGA AACCTTTCCC
    CCTCCTGAGG ACTTGCATGA TAAGTTTGTG ACAAAGATGT CCAGCTTTGT TATCAGCGCA
    AAAGAACAAT ATGAGAAACT TCTAAAGTTA CATGAAAATA TGGAGAAGTT ATACCAGAGT
    GTGATGGGAT ACTATGCCAT TGACGTGAAG AAGATGTCCG TGGAAGACTT TTTTAATGAC
    TTGAATAATT TCAGAACCAC ATTCATGCAA GCAAGAAAGG AGAACATCAA AAAAAGAGAA
    GCAGAGGAAA GGGAAAAACG TGCCCAAATA GCCAAAGAAT TGGCAGAGAA AGAAAGACTT
    GAACGCCAAC AAAAGAAAAA GCGCTTGTTA GAAATGAAGA CTGAGGGTGA TGAGACAGGA
    GTGATGGATA GTCTGCTGGA GGCTTTGCAG TCGGGCGCTG CCTTCCGCGA CCGAAGAAAA
    AGGACGCCAA AGCCAAAAGA TATTCGGCAA AGTCTCAGTC CCATGTCCCA GAGGCCTGTT
    CTGAAAGTTT GTAACCATGA AAATCAGAAA GTGCAGTTGA CAGAAGGGTC ACGTTCACAC
    TACAATATCA ATTGCAACTC AACGAGGACT CCAGTCGCCA AGGAGCTTAA TTATAATCTA
    GACACTCATA CGTCTACAGG GAGGATCAAG GCAGTTGAGA AGAAGGAAGC CTGTAATGTA
    GAAAGCAACA GAAAAAAGGA AATGGAACTT CTTGGCTCGG TTTCTAAAAA CGAATCTGTT
    CCCGAAGTTG AAGCCCTGCT GGCAAGATTA CGAGCTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634008.1
    CDS84..3065
    Translation

    Target ORF information:

    RefSeq Version XM_005633951.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633951.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTTTCAA GTTCAAGGAA CAGTTGGGTA GAAAGCTTTG GACGCGAGGG GCTTGGATTA 
    TTACTGGACA TTTTGGAAAG ACTGATTAGT GGCAAAATAC AAGAGAAAAT TGTAAAGAAG
    AATCAGCATA AAGTCATACA GTGTCTAAAG GCTTTGATGA ATACACAGTA TGGCTTGGAA
    AGAATTATGA GTGAAGAGAG GAGTCTTTCT CTATTAGCCA AAGCCATCGA TCCTGAGCAC
    CCAAGTATGA TGACAGATGT GGTGAAACTC CTCTCTGCGG TATGCATTGT AGGAGAGGAA
    AGCATTCTCG AAGAGGTTTT AGAAGCTTTA ACATCAGCTG GAGAAGAAAG AAAAATCGAC
    AGATTTTCAT CCATTGTGGA AGGTCTCCGG CATGATTCAG TTCAACTGCA AGTAGCGTGT
    ATGCAGCTCA TCAATGCCCT TGTTACATCT CCTGATGATT TGGACTTCAG GCTTCACATC
    AGAAATGAAT TTATGCGTTG TGGATTGAAA GAGATCTTGC CAGATTTAAA ATGTATGAAG
    AATGATAGCC TGGATATCCA ACTTAAAGTC TTTGATGAGC ATAAGGAAGA AGATTTGCTT
    GAGTTCTCCC ATCGCCTTGA AGATATTAGA GCTGAACTTG ATGAAGCATA TGATATTTAC
    AACATGGTGT GGAACTCAGT TAAGGAAACT AAAGCAGAGG GATATTTCAT TTCTATTCTT
    CAGCACCTTT TGCTGATTCG AAATGATTAT AGTATAAGGC AACAGTACTT CAAATTAATT
    GATGAGTGTA TATCTCAGAT TGTATTGCAT AGAGATGGGA TGGATCCAGA CTTCACATAT
    CGGAAAAGAC TGGATTTAGA TTTAAGTCAG TTTGTTGATA CTTGCATAGA TCAAGCAAAA
    CTAGAAGAGT TTGAAGAGAA AGCGTCAGAA CTGCACAAGA AATTTGAAAA AGAGTTTACC
    GACCACCAAG AAACTCAGGC TCAATTGCAG AAAAAAGAAG CAAAGATTAA TGAACTTCAA
    GCAGAGTTAC AAGCTTTTAA GTCAGAGTTT GGTGCCTTGC CAGCTGGTAG AAATCACTCT
    TTGCCACAGT CAAAAGAAAA TGGAACTGGC CCCCCAGCAC TTGCTCCTCC CCTGCCACTG
    CCATCTTGTG GAGGCATGCC GCCTCCACCC CCTCCTCCCC CACCTCCTCC GCTTCCAGGA
    ATGCCAATGC CTTTTGGTGG TCCCGTGCCT CCTCCACCAC CTCCTCTGGG ATTTCTCAGT
    GGGCGAAACT CTCCTCCTCC ACCAACCCTA CCATTTGGGT TAAAACCAAA GAAAGAATTT
    AAACCTGAAA CCAGCATGAG AAGATTGAAT TGGTTAAAGA TCAGACCTCA CGAAATGACT
    GAAAGCTGTT TCTGGATCAA AGTAAATGAA AATAAGTATG AAAATGTGGA TTTGCTCTGT
    AAACTTGAGA ATACGTTTTG TTGCCAACAA AAAGAGAGAA GAGAAGAAGA AGATTTTGAA
    GAAAAGAAAG CTATTAAGAA AAAAATTAAA GAGCTTAAAT TTTTAGATTC TAAAATTGCC
    CAGAATCTTT CCATCTTCCT GAGCTCTTTT CGGGTGCCAT ATGAGGAAAT CAAAACGATG
    ATATTGGAAG TGGATGAAAC ACAGTTGGCA GAGTCTATGA TTCAGAACCT AATAAAGCAT
    CTTCCTGATC AAGAACAATT AAGCTCACTG TCTCAGTTCA AGAATGACTA TAACAACCTA
    TGTGAACCCG AGCAGTTTGC TGTTGTGATG AGCAATGTGA AGAGACTACG GCCACGGCTC
    AGTGCTATTC TCTTTAAGCT TCAGTTTGAA GAGCAGGTGA ACAACATCAA ACCTGACATC
    ATGGCTGTCA GTACTGCCTG CGAGGAGATA AAGAAGAGCA AAAGCTTCAG CAAGTTGCTG
    GAACTTGTAT TGCTAATGGG AAACTACATG AATGCTGGCT CCCGGAATGC TCAAACCTTC
    GGATTTAACC TTAGCTCTCT CTGTAAACTA AAGGACACGA AATCAGCAGA TCAGAAAACA
    ACACTACTTC ATTTTCTGGT GGAAGTATGT GAAGAAAAGT ATCCTGATAT CCTGAATTTT
    GTGGATGATT TGGAACATTT AGACAAAGCT AGTAAAGTCT CTGTAGAAAC GCTGGAAAAG
    AATTTGAAGC AGATGGGAAG GCAGCTTCAA CAGCTTGAGA AAGATTTGGA AACCTTTCCC
    CCTCCTGAGG ACTTGCATGA TAAGTTTGTG ACAAAGATGT CCAGCTTTGT TATCAGCGCA
    AAAGAACAAT ATGAGAAACT TCTAAAGTTA CATGAAAATA TGGAGAAGTT ATACCAGAGT
    GTGATGGGAT ACTATGCCAT TGACGTGAAG AAGATGTCCG TGGAAGACTT TTTTAATGAC
    TTGAATAATT TCAGAACCAC ATTCATGCAA GCAAGAAAGG AGAACATCAA AAAAAGAGAA
    GCAGAGGAAA GGGAAAAACG TGCCCAAATA GCCAAAGAAT TGGCAGAGAA AGAAAGACTT
    GAACGCCAAC AAAAGAAAAA GCGCTTGTTA GAAATGAAGA CTGAGGGTGA TGAGACAGGA
    GTGATGGATA GTCTGCTGGA GGCTTTGCAG TCGGGCGCTG CCTTCCGCGA CCGAAGAAAA
    AGGACGCCAA AGCCAAAAGA TATTCGGCAA AGTCTCAGTC CCATGTCCCA GAGGCCTGTT
    CTGAAAGTTT GTAACCATGA AAATCAGAAA GTGCAGTTGA CAGAAGGGTC ACGTTCACAC
    TACAATATCA ATTGCAACTC AACGAGGACT CCAGTCGCCA AGGAGCTTAA TTATAATCTA
    GACACTCATA CGTCTACAGG GAGGATCAAG GCAGTTGAGA AGAAGGAAGC CTGTAATGTA
    GAAAGCAACA GAAAAAAGGA AATGGAACTT CTTGGCTCGG TTTCTAAAAA CGAATCTGTT
    CCCGAAGTTG AAGCCCTGCT GGCAAGATTA CGAGCTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23598
    Clone ID Related Accession (Same CDS sequence) XM_005633952.2 , XM_005633952.3
    Accession Version XM_005633952.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein diaphanous homolog 3 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAACAA GTTGGGTAGA AAGCTTTGGA CGCGAGGGGC TTGGATTATT ACTGGACATT 
    TTGGAAAGAC TGATTAGTGG CAAAATACAA GAGAAAATTG TAAAGAAGAA TCAGCATAAA
    GTCATACAGT GTCTAAAGGC TTTGATGAAT ACACAGTATG GCTTGGAAAG AATTATGAGT
    GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA GCCATCGATC CTGAGCACCC AAGTATGATG
    ACAGATGTGG TGAAACTCCT CTCTGCGGTA TGCATTGTAG GAGAGGAAAG CATTCTCGAA
    GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA GAAGAAAGAA AAATCGACAG ATTTTCATCC
    ATTGTGGAAG GTCTCCGGCA TGATTCAGTT CAACTGCAAG TAGCGTGTAT GCAGCTCATC
    AATGCCCTTG TTACATCTCC TGATGATTTG GACTTCAGGC TTCACATCAG AAATGAATTT
    ATGCGTTGTG GATTGAAAGA GATCTTGCCA GATTTAAAAT GTATGAAGAA TGATAGCCTG
    GATATCCAAC TTAAAGTCTT TGATGAGCAT AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT
    CGCCTTGAAG ATATTAGAGC TGAACTTGAT GAAGCATATG ATATTTACAA CATGGTGTGG
    AACTCAGTTA AGGAAACTAA AGCAGAGGGA TATTTCATTT CTATTCTTCA GCACCTTTTG
    CTGATTCGAA ATGATTATAG TATAAGGCAA CAGTACTTCA AATTAATTGA TGAGTGTATA
    TCTCAGATTG TATTGCATAG AGATGGGATG GATCCAGACT TCACATATCG GAAAAGACTG
    GATTTAGATT TAAGTCAGTT TGTTGATACT TGCATAGATC AAGCAAAACT AGAAGAGTTT
    GAAGAGAAAG CGTCAGAACT GCACAAGAAA TTTGAAAAAG AGTTTACCGA CCACCAAGAA
    ACTCAGGCTC AATTGCAGAA AAAAGAAGCA AAGATTAATG AACTTCAAGC AGAGTTACAA
    GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA GCTGGTAGAA ATCACTCTTT GCCACAGTCA
    AAAGAAAATG GAACTGGCCC CCCAGCACTT GCTCCTCCCC TGCCACTGCC ATCTTGTGGA
    GGCATGCCGC CTCCACCCCC TCCTCCCCCA CCTCCTCCGC TTCCAGGAAT GCCAATGCCT
    TTTGGTGGTC CCGTGCCTCC TCCACCACCT CCTCTGGGAT TTCTCAGTGG GCGAAACTCT
    CCTCCTCCAC CAACCCTACC ATTTGGGTTA AAACCAAAGA AAGAATTTAA ACCTGAAACC
    AGCATGAGAA GATTGAATTG GTTAAAGATC AGACCTCACG AAATGACTGA AAGCTGTTTC
    TGGATCAAAG TAAATGAAAA TAAGTATGAA AATGTGGATT TGCTCTGTAA ACTTGAGAAT
    ACGTTTTGTT GCCAACAAAA AGAGAGAAGA GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT
    ATTAAGAAAA AAATTAAAGA GCTTAAATTT TTAGATTCTA AAATTGCCCA GAATCTTTCC
    ATCTTCCTGA GCTCTTTTCG GGTGCCATAT GAGGAAATCA AAACGATGAT ATTGGAAGTG
    GATGAAACAC AGTTGGCAGA GTCTATGATT CAGAACCTAA TAAAGCATCT TCCTGATCAA
    GAACAATTAA GCTCACTGTC TCAGTTCAAG AATGACTATA ACAACCTATG TGAACCCGAG
    CAGTTTGCTG TTGTGATGAG CAATGTGAAG AGACTACGGC CACGGCTCAG TGCTATTCTC
    TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC AACATCAAAC CTGACATCAT GGCTGTCAGT
    ACTGCCTGCG AGGAGATAAA GAAGAGCAAA AGCTTCAGCA AGTTGCTGGA ACTTGTATTG
    CTAATGGGAA ACTACATGAA TGCTGGCTCC CGGAATGCTC AAACCTTCGG ATTTAACCTT
    AGCTCTCTCT GTAAACTAAA GGACACGAAA TCAGCAGATC AGAAAACAAC ACTACTTCAT
    TTTCTGGTGG AAGTATGTGA AGAAAAGTAT CCTGATATCC TGAATTTTGT GGATGATTTG
    GAACATTTAG ACAAAGCTAG TAAAGTCTCT GTAGAAACGC TGGAAAAGAA TTTGAAGCAG
    ATGGGAAGGC AGCTTCAACA GCTTGAGAAA GATTTGGAAA CCTTTCCCCC TCCTGAGGAC
    TTGCATGATA AGTTTGTGAC AAAGATGTCC AGCTTTGTTA TCAGCGCAAA AGAACAATAT
    GAGAAACTTC TAAAGTTACA TGAAAATATG GAGAAGTTAT ACCAGAGTGT GATGGGATAC
    TATGCCATTG ACGTGAAGAA GATGTCCGTG GAAGACTTTT TTAATGACTT GAATAATTTC
    AGAACCACAT TCATGCAAGC AAGAAAGGAG AACATCAAAA AAAGAGAAGC AGAGGAAAGG
    GAAAAACGTG CCCAAATAGC CAAAGAATTG GCAGAGAAAG AAAGACTTGA ACGCCAACAA
    AAGAAAAAGC GCTTGTTAGA AATGAAGACT GAGGGTGATG AGACAGGAGT GATGGATAGT
    CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC TTCCGCGACC GAAGAAAAAG GACGCCAAAG
    CCAAAAGATA TTCGGCAAAG TCTCAGTCCC ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT
    AACCATGAAA ATCAGAAAGT GCAGTTGACA GAAGGGTCAC GTTCACACTA CAATATCAAT
    TGCAACTCAA CGAGGACTCC AGTCGCCAAG GAGCTTAATT ATAATCTAGA CACTCATACG
    TCTACAGGGA GGATCAAGGC AGTTGAGAAG AAGGAAGCCT GTAATGTAGA AAGCAACAGA
    AAAAAGGAAA TGGAACTTCT TGGCTCGGTT TCTAAAAACG AATCTGTTCC CGAAGTTGAA
    GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634009.1
    CDS104..3073
    Translation

    Target ORF information:

    RefSeq Version XM_005633952.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633952.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAACAA GTTGGGTAGA AAGCTTTGGA CGCGAGGGGC TTGGATTATT ACTGGACATT 
    TTGGAAAGAC TGATTAGTGG CAAAATACAA GAGAAAATTG TAAAGAAGAA TCAGCATAAA
    GTCATACAGT GTCTAAAGGC TTTGATGAAT ACACAGTATG GCTTGGAAAG AATTATGAGT
    GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA GCCATCGATC CTGAGCACCC AAGTATGATG
    ACAGATGTGG TGAAACTCCT CTCTGCGGTA TGCATTGTAG GAGAGGAAAG CATTCTCGAA
    GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA GAAGAAAGAA AAATCGACAG ATTTTCATCC
    ATTGTGGAAG GTCTCCGGCA TGATTCAGTT CAACTGCAAG TAGCGTGTAT GCAGCTCATC
    AATGCCCTTG TTACATCTCC TGATGATTTG GACTTCAGGC TTCACATCAG AAATGAATTT
    ATGCGTTGTG GATTGAAAGA GATCTTGCCA GATTTAAAAT GTATGAAGAA TGATAGCCTG
    GATATCCAAC TTAAAGTCTT TGATGAGCAT AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT
    CGCCTTGAAG ATATTAGAGC TGAACTTGAT GAAGCATATG ATATTTACAA CATGGTGTGG
    AACTCAGTTA AGGAAACTAA AGCAGAGGGA TATTTCATTT CTATTCTTCA GCACCTTTTG
    CTGATTCGAA ATGATTATAG TATAAGGCAA CAGTACTTCA AATTAATTGA TGAGTGTATA
    TCTCAGATTG TATTGCATAG AGATGGGATG GATCCAGACT TCACATATCG GAAAAGACTG
    GATTTAGATT TAAGTCAGTT TGTTGATACT TGCATAGATC AAGCAAAACT AGAAGAGTTT
    GAAGAGAAAG CGTCAGAACT GCACAAGAAA TTTGAAAAAG AGTTTACCGA CCACCAAGAA
    ACTCAGGCTC AATTGCAGAA AAAAGAAGCA AAGATTAATG AACTTCAAGC AGAGTTACAA
    GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA GCTGGTAGAA ATCACTCTTT GCCACAGTCA
    AAAGAAAATG GAACTGGCCC CCCAGCACTT GCTCCTCCCC TGCCACTGCC ATCTTGTGGA
    GGCATGCCGC CTCCACCCCC TCCTCCCCCA CCTCCTCCGC TTCCAGGAAT GCCAATGCCT
    TTTGGTGGTC CCGTGCCTCC TCCACCACCT CCTCTGGGAT TTCTCAGTGG GCGAAACTCT
    CCTCCTCCAC CAACCCTACC ATTTGGGTTA AAACCAAAGA AAGAATTTAA ACCTGAAACC
    AGCATGAGAA GATTGAATTG GTTAAAGATC AGACCTCACG AAATGACTGA AAGCTGTTTC
    TGGATCAAAG TAAATGAAAA TAAGTATGAA AATGTGGATT TGCTCTGTAA ACTTGAGAAT
    ACGTTTTGTT GCCAACAAAA AGAGAGAAGA GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT
    ATTAAGAAAA AAATTAAAGA GCTTAAATTT TTAGATTCTA AAATTGCCCA GAATCTTTCC
    ATCTTCCTGA GCTCTTTTCG GGTGCCATAT GAGGAAATCA AAACGATGAT ATTGGAAGTG
    GATGAAACAC AGTTGGCAGA GTCTATGATT CAGAACCTAA TAAAGCATCT TCCTGATCAA
    GAACAATTAA GCTCACTGTC TCAGTTCAAG AATGACTATA ACAACCTATG TGAACCCGAG
    CAGTTTGCTG TTGTGATGAG CAATGTGAAG AGACTACGGC CACGGCTCAG TGCTATTCTC
    TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC AACATCAAAC CTGACATCAT GGCTGTCAGT
    ACTGCCTGCG AGGAGATAAA GAAGAGCAAA AGCTTCAGCA AGTTGCTGGA ACTTGTATTG
    CTAATGGGAA ACTACATGAA TGCTGGCTCC CGGAATGCTC AAACCTTCGG ATTTAACCTT
    AGCTCTCTCT GTAAACTAAA GGACACGAAA TCAGCAGATC AGAAAACAAC ACTACTTCAT
    TTTCTGGTGG AAGTATGTGA AGAAAAGTAT CCTGATATCC TGAATTTTGT GGATGATTTG
    GAACATTTAG ACAAAGCTAG TAAAGTCTCT GTAGAAACGC TGGAAAAGAA TTTGAAGCAG
    ATGGGAAGGC AGCTTCAACA GCTTGAGAAA GATTTGGAAA CCTTTCCCCC TCCTGAGGAC
    TTGCATGATA AGTTTGTGAC AAAGATGTCC AGCTTTGTTA TCAGCGCAAA AGAACAATAT
    GAGAAACTTC TAAAGTTACA TGAAAATATG GAGAAGTTAT ACCAGAGTGT GATGGGATAC
    TATGCCATTG ACGTGAAGAA GATGTCCGTG GAAGACTTTT TTAATGACTT GAATAATTTC
    AGAACCACAT TCATGCAAGC AAGAAAGGAG AACATCAAAA AAAGAGAAGC AGAGGAAAGG
    GAAAAACGTG CCCAAATAGC CAAAGAATTG GCAGAGAAAG AAAGACTTGA ACGCCAACAA
    AAGAAAAAGC GCTTGTTAGA AATGAAGACT GAGGGTGATG AGACAGGAGT GATGGATAGT
    CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC TTCCGCGACC GAAGAAAAAG GACGCCAAAG
    CCAAAAGATA TTCGGCAAAG TCTCAGTCCC ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT
    AACCATGAAA ATCAGAAAGT GCAGTTGACA GAAGGGTCAC GTTCACACTA CAATATCAAT
    TGCAACTCAA CGAGGACTCC AGTCGCCAAG GAGCTTAATT ATAATCTAGA CACTCATACG
    TCTACAGGGA GGATCAAGGC AGTTGAGAAG AAGGAAGCCT GTAATGTAGA AAGCAACAGA
    AAAAAGGAAA TGGAACTTCT TGGCTCGGTT TCTAAAAACG AATCTGTTCC CGAAGTTGAA
    GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23598
    Clone ID Related Accession (Same CDS sequence) XM_005633952.2 , XM_005633952.3
    Accession Version XM_005633952.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAACAA GTTGGGTAGA AAGCTTTGGA CGCGAGGGGC TTGGATTATT ACTGGACATT 
    TTGGAAAGAC TGATTAGTGG CAAAATACAA GAGAAAATTG TAAAGAAGAA TCAGCATAAA
    GTCATACAGT GTCTAAAGGC TTTGATGAAT ACACAGTATG GCTTGGAAAG AATTATGAGT
    GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA GCCATCGATC CTGAGCACCC AAGTATGATG
    ACAGATGTGG TGAAACTCCT CTCTGCGGTA TGCATTGTAG GAGAGGAAAG CATTCTCGAA
    GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA GAAGAAAGAA AAATCGACAG ATTTTCATCC
    ATTGTGGAAG GTCTCCGGCA TGATTCAGTT CAACTGCAAG TAGCGTGTAT GCAGCTCATC
    AATGCCCTTG TTACATCTCC TGATGATTTG GACTTCAGGC TTCACATCAG AAATGAATTT
    ATGCGTTGTG GATTGAAAGA GATCTTGCCA GATTTAAAAT GTATGAAGAA TGATAGCCTG
    GATATCCAAC TTAAAGTCTT TGATGAGCAT AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT
    CGCCTTGAAG ATATTAGAGC TGAACTTGAT GAAGCATATG ATATTTACAA CATGGTGTGG
    AACTCAGTTA AGGAAACTAA AGCAGAGGGA TATTTCATTT CTATTCTTCA GCACCTTTTG
    CTGATTCGAA ATGATTATAG TATAAGGCAA CAGTACTTCA AATTAATTGA TGAGTGTATA
    TCTCAGATTG TATTGCATAG AGATGGGATG GATCCAGACT TCACATATCG GAAAAGACTG
    GATTTAGATT TAAGTCAGTT TGTTGATACT TGCATAGATC AAGCAAAACT AGAAGAGTTT
    GAAGAGAAAG CGTCAGAACT GCACAAGAAA TTTGAAAAAG AGTTTACCGA CCACCAAGAA
    ACTCAGGCTC AATTGCAGAA AAAAGAAGCA AAGATTAATG AACTTCAAGC AGAGTTACAA
    GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA GCTGGTAGAA ATCACTCTTT GCCACAGTCA
    AAAGAAAATG GAACTGGCCC CCCAGCACTT GCTCCTCCCC TGCCACTGCC ATCTTGTGGA
    GGCATGCCGC CTCCACCCCC TCCTCCCCCA CCTCCTCCGC TTCCAGGAAT GCCAATGCCT
    TTTGGTGGTC CCGTGCCTCC TCCACCACCT CCTCTGGGAT TTCTCAGTGG GCGAAACTCT
    CCTCCTCCAC CAACCCTACC ATTTGGGTTA AAACCAAAGA AAGAATTTAA ACCTGAAACC
    AGCATGAGAA GATTGAATTG GTTAAAGATC AGACCTCACG AAATGACTGA AAGCTGTTTC
    TGGATCAAAG TAAATGAAAA TAAGTATGAA AATGTGGATT TGCTCTGTAA ACTTGAGAAT
    ACGTTTTGTT GCCAACAAAA AGAGAGAAGA GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT
    ATTAAGAAAA AAATTAAAGA GCTTAAATTT TTAGATTCTA AAATTGCCCA GAATCTTTCC
    ATCTTCCTGA GCTCTTTTCG GGTGCCATAT GAGGAAATCA AAACGATGAT ATTGGAAGTG
    GATGAAACAC AGTTGGCAGA GTCTATGATT CAGAACCTAA TAAAGCATCT TCCTGATCAA
    GAACAATTAA GCTCACTGTC TCAGTTCAAG AATGACTATA ACAACCTATG TGAACCCGAG
    CAGTTTGCTG TTGTGATGAG CAATGTGAAG AGACTACGGC CACGGCTCAG TGCTATTCTC
    TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC AACATCAAAC CTGACATCAT GGCTGTCAGT
    ACTGCCTGCG AGGAGATAAA GAAGAGCAAA AGCTTCAGCA AGTTGCTGGA ACTTGTATTG
    CTAATGGGAA ACTACATGAA TGCTGGCTCC CGGAATGCTC AAACCTTCGG ATTTAACCTT
    AGCTCTCTCT GTAAACTAAA GGACACGAAA TCAGCAGATC AGAAAACAAC ACTACTTCAT
    TTTCTGGTGG AAGTATGTGA AGAAAAGTAT CCTGATATCC TGAATTTTGT GGATGATTTG
    GAACATTTAG ACAAAGCTAG TAAAGTCTCT GTAGAAACGC TGGAAAAGAA TTTGAAGCAG
    ATGGGAAGGC AGCTTCAACA GCTTGAGAAA GATTTGGAAA CCTTTCCCCC TCCTGAGGAC
    TTGCATGATA AGTTTGTGAC AAAGATGTCC AGCTTTGTTA TCAGCGCAAA AGAACAATAT
    GAGAAACTTC TAAAGTTACA TGAAAATATG GAGAAGTTAT ACCAGAGTGT GATGGGATAC
    TATGCCATTG ACGTGAAGAA GATGTCCGTG GAAGACTTTT TTAATGACTT GAATAATTTC
    AGAACCACAT TCATGCAAGC AAGAAAGGAG AACATCAAAA AAAGAGAAGC AGAGGAAAGG
    GAAAAACGTG CCCAAATAGC CAAAGAATTG GCAGAGAAAG AAAGACTTGA ACGCCAACAA
    AAGAAAAAGC GCTTGTTAGA AATGAAGACT GAGGGTGATG AGACAGGAGT GATGGATAGT
    CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC TTCCGCGACC GAAGAAAAAG GACGCCAAAG
    CCAAAAGATA TTCGGCAAAG TCTCAGTCCC ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT
    AACCATGAAA ATCAGAAAGT GCAGTTGACA GAAGGGTCAC GTTCACACTA CAATATCAAT
    TGCAACTCAA CGAGGACTCC AGTCGCCAAG GAGCTTAATT ATAATCTAGA CACTCATACG
    TCTACAGGGA GGATCAAGGC AGTTGAGAAG AAGGAAGCCT GTAATGTAGA AAGCAACAGA
    AAAAAGGAAA TGGAACTTCT TGGCTCGGTT TCTAAAAACG AATCTGTTCC CGAAGTTGAA
    GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634009.1
    CDS132..3101
    Translation

    Target ORF information:

    RefSeq Version XM_005633952.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633952.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCAACAA GTTGGGTAGA AAGCTTTGGA CGCGAGGGGC TTGGATTATT ACTGGACATT 
    TTGGAAAGAC TGATTAGTGG CAAAATACAA GAGAAAATTG TAAAGAAGAA TCAGCATAAA
    GTCATACAGT GTCTAAAGGC TTTGATGAAT ACACAGTATG GCTTGGAAAG AATTATGAGT
    GAAGAGAGGA GTCTTTCTCT ATTAGCCAAA GCCATCGATC CTGAGCACCC AAGTATGATG
    ACAGATGTGG TGAAACTCCT CTCTGCGGTA TGCATTGTAG GAGAGGAAAG CATTCTCGAA
    GAGGTTTTAG AAGCTTTAAC ATCAGCTGGA GAAGAAAGAA AAATCGACAG ATTTTCATCC
    ATTGTGGAAG GTCTCCGGCA TGATTCAGTT CAACTGCAAG TAGCGTGTAT GCAGCTCATC
    AATGCCCTTG TTACATCTCC TGATGATTTG GACTTCAGGC TTCACATCAG AAATGAATTT
    ATGCGTTGTG GATTGAAAGA GATCTTGCCA GATTTAAAAT GTATGAAGAA TGATAGCCTG
    GATATCCAAC TTAAAGTCTT TGATGAGCAT AAGGAAGAAG ATTTGCTTGA GTTCTCCCAT
    CGCCTTGAAG ATATTAGAGC TGAACTTGAT GAAGCATATG ATATTTACAA CATGGTGTGG
    AACTCAGTTA AGGAAACTAA AGCAGAGGGA TATTTCATTT CTATTCTTCA GCACCTTTTG
    CTGATTCGAA ATGATTATAG TATAAGGCAA CAGTACTTCA AATTAATTGA TGAGTGTATA
    TCTCAGATTG TATTGCATAG AGATGGGATG GATCCAGACT TCACATATCG GAAAAGACTG
    GATTTAGATT TAAGTCAGTT TGTTGATACT TGCATAGATC AAGCAAAACT AGAAGAGTTT
    GAAGAGAAAG CGTCAGAACT GCACAAGAAA TTTGAAAAAG AGTTTACCGA CCACCAAGAA
    ACTCAGGCTC AATTGCAGAA AAAAGAAGCA AAGATTAATG AACTTCAAGC AGAGTTACAA
    GCTTTTAAGT CAGAGTTTGG TGCCTTGCCA GCTGGTAGAA ATCACTCTTT GCCACAGTCA
    AAAGAAAATG GAACTGGCCC CCCAGCACTT GCTCCTCCCC TGCCACTGCC ATCTTGTGGA
    GGCATGCCGC CTCCACCCCC TCCTCCCCCA CCTCCTCCGC TTCCAGGAAT GCCAATGCCT
    TTTGGTGGTC CCGTGCCTCC TCCACCACCT CCTCTGGGAT TTCTCAGTGG GCGAAACTCT
    CCTCCTCCAC CAACCCTACC ATTTGGGTTA AAACCAAAGA AAGAATTTAA ACCTGAAACC
    AGCATGAGAA GATTGAATTG GTTAAAGATC AGACCTCACG AAATGACTGA AAGCTGTTTC
    TGGATCAAAG TAAATGAAAA TAAGTATGAA AATGTGGATT TGCTCTGTAA ACTTGAGAAT
    ACGTTTTGTT GCCAACAAAA AGAGAGAAGA GAAGAAGAAG ATTTTGAAGA AAAGAAAGCT
    ATTAAGAAAA AAATTAAAGA GCTTAAATTT TTAGATTCTA AAATTGCCCA GAATCTTTCC
    ATCTTCCTGA GCTCTTTTCG GGTGCCATAT GAGGAAATCA AAACGATGAT ATTGGAAGTG
    GATGAAACAC AGTTGGCAGA GTCTATGATT CAGAACCTAA TAAAGCATCT TCCTGATCAA
    GAACAATTAA GCTCACTGTC TCAGTTCAAG AATGACTATA ACAACCTATG TGAACCCGAG
    CAGTTTGCTG TTGTGATGAG CAATGTGAAG AGACTACGGC CACGGCTCAG TGCTATTCTC
    TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC AACATCAAAC CTGACATCAT GGCTGTCAGT
    ACTGCCTGCG AGGAGATAAA GAAGAGCAAA AGCTTCAGCA AGTTGCTGGA ACTTGTATTG
    CTAATGGGAA ACTACATGAA TGCTGGCTCC CGGAATGCTC AAACCTTCGG ATTTAACCTT
    AGCTCTCTCT GTAAACTAAA GGACACGAAA TCAGCAGATC AGAAAACAAC ACTACTTCAT
    TTTCTGGTGG AAGTATGTGA AGAAAAGTAT CCTGATATCC TGAATTTTGT GGATGATTTG
    GAACATTTAG ACAAAGCTAG TAAAGTCTCT GTAGAAACGC TGGAAAAGAA TTTGAAGCAG
    ATGGGAAGGC AGCTTCAACA GCTTGAGAAA GATTTGGAAA CCTTTCCCCC TCCTGAGGAC
    TTGCATGATA AGTTTGTGAC AAAGATGTCC AGCTTTGTTA TCAGCGCAAA AGAACAATAT
    GAGAAACTTC TAAAGTTACA TGAAAATATG GAGAAGTTAT ACCAGAGTGT GATGGGATAC
    TATGCCATTG ACGTGAAGAA GATGTCCGTG GAAGACTTTT TTAATGACTT GAATAATTTC
    AGAACCACAT TCATGCAAGC AAGAAAGGAG AACATCAAAA AAAGAGAAGC AGAGGAAAGG
    GAAAAACGTG CCCAAATAGC CAAAGAATTG GCAGAGAAAG AAAGACTTGA ACGCCAACAA
    AAGAAAAAGC GCTTGTTAGA AATGAAGACT GAGGGTGATG AGACAGGAGT GATGGATAGT
    CTGCTGGAGG CTTTGCAGTC GGGCGCTGCC TTCCGCGACC GAAGAAAAAG GACGCCAAAG
    CCAAAAGATA TTCGGCAAAG TCTCAGTCCC ATGTCCCAGA GGCCTGTTCT GAAAGTTTGT
    AACCATGAAA ATCAGAAAGT GCAGTTGACA GAAGGGTCAC GTTCACACTA CAATATCAAT
    TGCAACTCAA CGAGGACTCC AGTCGCCAAG GAGCTTAATT ATAATCTAGA CACTCATACG
    TCTACAGGGA GGATCAAGGC AGTTGAGAAG AAGGAAGCCT GTAATGTAGA AAGCAACAGA
    AAAAAGGAAA TGGAACTTCT TGGCTCGGTT TCTAAAAACG AATCTGTTCC CGAAGTTGAA
    GCCCTGCTGG CAAGATTACG AGCTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23592
    Clone ID Related Accession (Same CDS sequence) XM_005633942.2
    Accession Version XM_005633942.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein diaphanous homolog 3 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633942.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGGATA AATTTGCCAG CATAAGAATT CCAGGGAGCA AGAAGGAGAG ACCTCCACTT 
    TCTAACCTGA AGACTGCATT TGCAAGCAAT GATTGCTCTT CAGAGGTGAT GGAAAACATT
    CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA GAACTTTTCG AAAAGATGAT GGAAGATATG
    AATTTAAATG AAGATAAGAA GGCACCATTG CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA
    ATGGTGATGC AGTACATTAA TACTGCTTCT AAGACAGGAA GTTTTAAGAG TAAGCGCCAG
    ATCTCACCTC AGGAATTCAT CCATGAACTG AAAATGGGGT CTGCAGATGA GAGACTTGTA
    ACATGCCTGG AGTCCCTCCG TGTGTCTTTG ACTAGTAATC CTGTGAGTTG GGTAGAAAGC
    TTTGGACGCG AGGGGCTTGG ATTATTACTG GACATTTTGG AAAGACTGAT TAGTGGCAAA
    ATACAAGAGA AAATTGTAAA GAAGAATCAG CATAAAGTCA TACAGTGTCT AAAGGCTTTG
    ATGAATACAC AGTATGGCTT GGAAAGAATT ATGAGTGAAG AGAGGAGTCT TTCTCTATTA
    GCCAAAGCCA TCGATCCTGA GCACCCAAGT ATGATGACAG ATGTGGTGAA ACTCCTCTCT
    GCGGTATGCA TTGTAGGAGA GGAAAGCATT CTCGAAGAGG TTTTAGAAGC TTTAACATCA
    GCTGGAGAAG AAAGAAAAAT CGACAGATTT TCATCCATTG TGGAAGGTCT CCGGCATGAT
    TCAGTTCAAC TGCAAGTAGC GTGTATGCAG CTCATCAATG CCCTTGTTAC ATCTCCTGAT
    GATTTGGACT TCAGGCTTCA CATCAGAAAT GAATTTATGC GTTGTGGATT GAAAGAGATC
    TTGCCAGATT TAAAATGTAT GAAGAATGAT AGCCTGGATA TCCAACTTAA AGTCTTTGAT
    GAGCATAAGG AAGAAGATTT GCTTGAGTTC TCCCATCGCC TTGAAGATAT TAGAGCTGAA
    CTTGATGAAG CATATGATAT TTACAACATG GTGTGGAACT CAGTTAAGGA AACTAAAGCA
    GAGGGATATT TCATTTCTAT TCTTCAGCAC CTTTTGCTGA TTCGAAATGA TTATAGTATA
    AGGCAACAGT ACTTCAAATT AATTGATGAG TGTATATCTC AGATTGTATT GCATAGAGAT
    GGGATGGATC CAGACTTCAC ATATCGGAAA AGACTGGATT TAGATTTAAG TCAGTTTGTT
    GATACTTGCA TAGATCAAGC AAAACTAGAA GAGTTTGAAG AGAAAGCGTC AGAACTGCAC
    AAGAAATTTG AAAAAGAGTT TACCGACCAC CAAGAAACTC AGGCTCAATT GCAGAAAAAA
    GAAGCAAAGA TTAATGAACT TCAAGCAGAG TTACAAGCTT TTAAGTCAGA GTTTGGTGCC
    TTGCCAGCTG GTAGAAATCA CTCTTTGCCA CAGTCAAAAG AAAATGGAAC TGGCCCCCCA
    GCACTTGCTC CTCCCCTGCC ACTGCCATCT TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT
    CCCCCACCTC CTCCGCTTCC AGGAATGCCA ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA
    CCACCTCCTC TGGGATTTCT CAGTGGGCGA AACTCTCCTC CTCCACCAAC CCTACCATTT
    GGGTTAAAAC CAAAGAAAGA ATTTAAACCT GAAACCAGCA TGAGAAGATT GAATTGGTTA
    AAGATCAGAC CTCACGAAAT GACTGAAAGC TGTTTCTGGA TCAAAGTAAA TGAAAATAAG
    TATGAAAATG TGGATTTGCT CTGTAAACTT GAGAATACGT TTTGTTGCCA ACAAAAAGAG
    AGAAGAGAAG AAGAAGATTT TGAAGAAAAG AAAGCTATTA AGAAAAAAAT TAAAGAGCTT
    AAATTTTTAG ATTCTAAAAT TGCCCAGAAT CTTTCCATCT TCCTGAGCTC TTTTCGGGTG
    CCATATGAGG AAATCAAAAC GATGATATTG GAAGTGGATG AAACACAGTT GGCAGAGTCT
    ATGATTCAGA ACCTAATAAA GCATCTTCCT GATCAAGAAC AATTAAGCTC ACTGTCTCAG
    TTCAAGAATG ACTATAACAA CCTATGTGAA CCCGAGCAGT TTGCTGTTGT GATGAGCAAT
    GTGAAGAGAC TACGGCCACG GCTCAGTGCT ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG
    GTGAACAACA TCAAACCTGA CATCATGGCT GTCAGTACTG CCTGCGAGGA GATAAAGAAG
    AGCAAAAGCT TCAGCAAGTT GCTGGAACTT GTATTGCTAA TGGGAAACTA CATGAATGCT
    GGCTCCCGGA ATGCTCAAAC CTTCGGATTT AACCTTAGCT CTCTCTGTAA ACTAAAGGAC
    ACGAAATCAG CAGATCAGAA AACAACACTA CTTCATTTTC TGGTGGAAGT ATGTGAAGAA
    AAGTATCCTG ATATCCTGAA TTTTGTGGAT GATTTGGAAC ATTTAGACAA AGCTAGTAAA
    GTCTCTGTAG AAACGCTGGA AAAGAATTTG AAGCAGATGG GAAGGCAGCT TCAACAGCTT
    GAGAAAGATT TGGAAACCTT TCCCCCTCCT GAGGACTTGC ATGATAAGTT TGTGACAAAG
    ATGTCCAGCT TTGTTATCAG CGCAAAAGAA CAATATGAGA AACTTCTAAA GTTACATGAA
    AATATGGAGA AGTTATACCA GAGTGTGATG GGATACTATG CCATTGACGT GAAGAAGATG
    TCCGTGGAAG ACTTTTTTAA TGACTTGAAT AATTTCAGAA CCACATTCAT GCAAGCAAGA
    AAGGAGAACA TCAAAAAAAG AGAAGCAGAG GAAAGGGAAA AACGTGCCCA AATAGCCAAA
    GAATTGGCAG AGAAAGAAAG ACTTGAACGC CAACAAAAGA AAAAGCGCTT GTTAGAAATG
    AAGACTGAGG GTGATGAGAC AGGAGTGATG GATAGTCTGC TGGAGGCTTT GCAGTCGGGC
    GCTGCCTTCC GCGACCGAAG AAAAAGGACG CCAAAGCCAA AAGATATTCG GCAAAGTCTC
    AGTCCCATGT CCCAGAGGCC TGTTCTGAAA GTTTGTAACC ATGAAAATCA GAAAGTGCAG
    TTGACAGAAG GGTCACGTTC ACACTACAAT ATCAATTGCA ACTCAACGAG GACTCCAGTC
    GCCAAGGAGC TTAATTATAA TCTAGACACT CATACGTCTA CAGGGAGGAT CAAGGCAGTT
    GAGAAGAAGG AAGCCTGTAA TGTAGAAAGC AACAGAAAAA AGGAAATGGA ACTTCTTGGC
    TCGGTTTCTA AAAACGAATC TGTTCCCGAA GTTGAAGCCC TGCTGGCAAG ATTACGAGCT
    TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005633999.1
    CDS229..3594
    Translation

    Target ORF information:

    RefSeq Version XM_005633942.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633942.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGGATA AATTTGCCAG CATAAGAATT CCAGGGAGCA AGAAGGAGAG ACCTCCACTT 
    TCTAACCTGA AGACTGCATT TGCAAGCAAT GATTGCTCTT CAGAGGTGAT GGAAAACATT
    CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA GAACTTTTCG AAAAGATGAT GGAAGATATG
    AATTTAAATG AAGATAAGAA GGCACCATTG CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA
    ATGGTGATGC AGTACATTAA TACTGCTTCT AAGACAGGAA GTTTTAAGAG TAAGCGCCAG
    ATCTCACCTC AGGAATTCAT CCATGAACTG AAAATGGGGT CTGCAGATGA GAGACTTGTA
    ACATGCCTGG AGTCCCTCCG TGTGTCTTTG ACTAGTAATC CTGTGAGTTG GGTAGAAAGC
    TTTGGACGCG AGGGGCTTGG ATTATTACTG GACATTTTGG AAAGACTGAT TAGTGGCAAA
    ATACAAGAGA AAATTGTAAA GAAGAATCAG CATAAAGTCA TACAGTGTCT AAAGGCTTTG
    ATGAATACAC AGTATGGCTT GGAAAGAATT ATGAGTGAAG AGAGGAGTCT TTCTCTATTA
    GCCAAAGCCA TCGATCCTGA GCACCCAAGT ATGATGACAG ATGTGGTGAA ACTCCTCTCT
    GCGGTATGCA TTGTAGGAGA GGAAAGCATT CTCGAAGAGG TTTTAGAAGC TTTAACATCA
    GCTGGAGAAG AAAGAAAAAT CGACAGATTT TCATCCATTG TGGAAGGTCT CCGGCATGAT
    TCAGTTCAAC TGCAAGTAGC GTGTATGCAG CTCATCAATG CCCTTGTTAC ATCTCCTGAT
    GATTTGGACT TCAGGCTTCA CATCAGAAAT GAATTTATGC GTTGTGGATT GAAAGAGATC
    TTGCCAGATT TAAAATGTAT GAAGAATGAT AGCCTGGATA TCCAACTTAA AGTCTTTGAT
    GAGCATAAGG AAGAAGATTT GCTTGAGTTC TCCCATCGCC TTGAAGATAT TAGAGCTGAA
    CTTGATGAAG CATATGATAT TTACAACATG GTGTGGAACT CAGTTAAGGA AACTAAAGCA
    GAGGGATATT TCATTTCTAT TCTTCAGCAC CTTTTGCTGA TTCGAAATGA TTATAGTATA
    AGGCAACAGT ACTTCAAATT AATTGATGAG TGTATATCTC AGATTGTATT GCATAGAGAT
    GGGATGGATC CAGACTTCAC ATATCGGAAA AGACTGGATT TAGATTTAAG TCAGTTTGTT
    GATACTTGCA TAGATCAAGC AAAACTAGAA GAGTTTGAAG AGAAAGCGTC AGAACTGCAC
    AAGAAATTTG AAAAAGAGTT TACCGACCAC CAAGAAACTC AGGCTCAATT GCAGAAAAAA
    GAAGCAAAGA TTAATGAACT TCAAGCAGAG TTACAAGCTT TTAAGTCAGA GTTTGGTGCC
    TTGCCAGCTG GTAGAAATCA CTCTTTGCCA CAGTCAAAAG AAAATGGAAC TGGCCCCCCA
    GCACTTGCTC CTCCCCTGCC ACTGCCATCT TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT
    CCCCCACCTC CTCCGCTTCC AGGAATGCCA ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA
    CCACCTCCTC TGGGATTTCT CAGTGGGCGA AACTCTCCTC CTCCACCAAC CCTACCATTT
    GGGTTAAAAC CAAAGAAAGA ATTTAAACCT GAAACCAGCA TGAGAAGATT GAATTGGTTA
    AAGATCAGAC CTCACGAAAT GACTGAAAGC TGTTTCTGGA TCAAAGTAAA TGAAAATAAG
    TATGAAAATG TGGATTTGCT CTGTAAACTT GAGAATACGT TTTGTTGCCA ACAAAAAGAG
    AGAAGAGAAG AAGAAGATTT TGAAGAAAAG AAAGCTATTA AGAAAAAAAT TAAAGAGCTT
    AAATTTTTAG ATTCTAAAAT TGCCCAGAAT CTTTCCATCT TCCTGAGCTC TTTTCGGGTG
    CCATATGAGG AAATCAAAAC GATGATATTG GAAGTGGATG AAACACAGTT GGCAGAGTCT
    ATGATTCAGA ACCTAATAAA GCATCTTCCT GATCAAGAAC AATTAAGCTC ACTGTCTCAG
    TTCAAGAATG ACTATAACAA CCTATGTGAA CCCGAGCAGT TTGCTGTTGT GATGAGCAAT
    GTGAAGAGAC TACGGCCACG GCTCAGTGCT ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG
    GTGAACAACA TCAAACCTGA CATCATGGCT GTCAGTACTG CCTGCGAGGA GATAAAGAAG
    AGCAAAAGCT TCAGCAAGTT GCTGGAACTT GTATTGCTAA TGGGAAACTA CATGAATGCT
    GGCTCCCGGA ATGCTCAAAC CTTCGGATTT AACCTTAGCT CTCTCTGTAA ACTAAAGGAC
    ACGAAATCAG CAGATCAGAA AACAACACTA CTTCATTTTC TGGTGGAAGT ATGTGAAGAA
    AAGTATCCTG ATATCCTGAA TTTTGTGGAT GATTTGGAAC ATTTAGACAA AGCTAGTAAA
    GTCTCTGTAG AAACGCTGGA AAAGAATTTG AAGCAGATGG GAAGGCAGCT TCAACAGCTT
    GAGAAAGATT TGGAAACCTT TCCCCCTCCT GAGGACTTGC ATGATAAGTT TGTGACAAAG
    ATGTCCAGCT TTGTTATCAG CGCAAAAGAA CAATATGAGA AACTTCTAAA GTTACATGAA
    AATATGGAGA AGTTATACCA GAGTGTGATG GGATACTATG CCATTGACGT GAAGAAGATG
    TCCGTGGAAG ACTTTTTTAA TGACTTGAAT AATTTCAGAA CCACATTCAT GCAAGCAAGA
    AAGGAGAACA TCAAAAAAAG AGAAGCAGAG GAAAGGGAAA AACGTGCCCA AATAGCCAAA
    GAATTGGCAG AGAAAGAAAG ACTTGAACGC CAACAAAAGA AAAAGCGCTT GTTAGAAATG
    AAGACTGAGG GTGATGAGAC AGGAGTGATG GATAGTCTGC TGGAGGCTTT GCAGTCGGGC
    GCTGCCTTCC GCGACCGAAG AAAAAGGACG CCAAAGCCAA AAGATATTCG GCAAAGTCTC
    AGTCCCATGT CCCAGAGGCC TGTTCTGAAA GTTTGTAACC ATGAAAATCA GAAAGTGCAG
    TTGACAGAAG GGTCACGTTC ACACTACAAT ATCAATTGCA ACTCAACGAG GACTCCAGTC
    GCCAAGGAGC TTAATTATAA TCTAGACACT CATACGTCTA CAGGGAGGAT CAAGGCAGTT
    GAGAAGAAGG AAGCCTGTAA TGTAGAAAGC AACAGAAAAA AGGAAATGGA ACTTCTTGGC
    TCGGTTTCTA AAAACGAATC TGTTCCCGAA GTTGAAGCCC TGCTGGCAAG ATTACGAGCT
    TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23589
    Clone ID Related Accession (Same CDS sequence) XM_542593.5 , XM_542593.6
    Accession Version XM_542593.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein diaphanous homolog 3 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_542593.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGAAAATCA GAAAGTGCAG TTGACAGAAG GGTCACGTTC ACACTACAAT
    ATCAATTGCA ACTCAACGAG GACTCCAGTC GCCAAGGAGC TTAATTATAA TCTAGACACT
    CATACGTCTA CAGGGAGGAT CAAGGCAGTT GAGAAGAAGG AAGCCTGTAA TGTAGAAAGC
    AACAGAAAAA AGGAAATGGA ACTTCTTGGC TCGGTTTCTA AAAACGAATC TGTTCCCGAA
    GTTGAAGCCC TGCTGGCAAG ATTACGAGCT TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_542593.3
    CDS133..3708
    Translation

    Target ORF information:

    RefSeq Version XM_542593.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_542593.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGAAAATCA GAAAGTGCAG TTGACAGAAG GGTCACGTTC ACACTACAAT
    ATCAATTGCA ACTCAACGAG GACTCCAGTC GCCAAGGAGC TTAATTATAA TCTAGACACT
    CATACGTCTA CAGGGAGGAT CAAGGCAGTT GAGAAGAAGG AAGCCTGTAA TGTAGAAAGC
    AACAGAAAAA AGGAAATGGA ACTTCTTGGC TCGGTTTCTA AAAACGAATC TGTTCCCGAA
    GTTGAAGCCC TGCTGGCAAG ATTACGAGCT TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23589
    Clone ID Related Accession (Same CDS sequence) XM_542593.5 , XM_542593.6
    Accession Version XM_542593.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_542593.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGAAAATCA GAAAGTGCAG TTGACAGAAG GGTCACGTTC ACACTACAAT
    ATCAATTGCA ACTCAACGAG GACTCCAGTC GCCAAGGAGC TTAATTATAA TCTAGACACT
    CATACGTCTA CAGGGAGGAT CAAGGCAGTT GAGAAGAAGG AAGCCTGTAA TGTAGAAAGC
    AACAGAAAAA AGGAAATGGA ACTTCTTGGC TCGGTTTCTA AAAACGAATC TGTTCCCGAA
    GTTGAAGCCC TGCTGGCAAG ATTACGAGCT TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_542593.3
    CDS16..3591
    Translation

    Target ORF information:

    RefSeq Version XM_542593.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_542593.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGAAAATCA GAAAGTGCAG TTGACAGAAG GGTCACGTTC ACACTACAAT
    ATCAATTGCA ACTCAACGAG GACTCCAGTC GCCAAGGAGC TTAATTATAA TCTAGACACT
    CATACGTCTA CAGGGAGGAT CAAGGCAGTT GAGAAGAAGG AAGCCTGTAA TGTAGAAAGC
    AACAGAAAAA AGGAAATGGA ACTTCTTGGC TCGGTTTCTA AAAACGAATC TGTTCCCGAA
    GTTGAAGCCC TGCTGGCAAG ATTACGAGCT TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23590
    Clone ID Related Accession (Same CDS sequence) XM_005633940.2 , XM_005633940.3
    Accession Version XM_005633940.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein diaphanous homolog 3 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CTGGATAAAT TTGCCAGCAT AAGAATTCCA GGGAGCAAGA AGGAGAGACC TCCACTTTCT
    AACCTGAAGA CTGCATTTGC AAGCAATGAT TGCTCTTCAG AGGTGATGGA AAACATTCCA
    AAGTCACTGT CAGAGAATGA AGTCTTAGAA CTTTTCGAAA AGATGATGGA AGATATGAAT
    TTAAATGAAG ATAAGAAGGC ACCATTGCGG GAAAAGGACT TCAGCATCAA AAAAGAAATG
    GTGATGCAGT ACATTAATAC TGCTTCTAAG ACAGGAAGTT TTAAGAGTAA GCGCCAGATC
    TCACCTCAGG AATTCATCCA TGAACTGAAA ATGGGGTCTG CAGATGAGAG ACTTGTAACA
    TGCCTGGAGT CCCTCCGTGT GTCTTTGACT AGTAATCCTG TGAGTTGGGT AGAAAGCTTT
    GGACGCGAGG GGCTTGGATT ATTACTGGAC ATTTTGGAAA GACTGATTAG TGGCAAAATA
    CAAGAGAAAA TTGTAAAGAA GAATCAGCAT AAAGTCATAC AGTGTCTAAA GGCTTTGATG
    AATACACAGT ATGGCTTGGA AAGAATTATG AGTGAAGAGA GGAGTCTTTC TCTATTAGCC
    AAAGCCATCG ATCCTGAGCA CCCAAGTATG ATGACAGATG TGGTGAAACT CCTCTCTGCG
    GTATGCATTG TAGGAGAGGA AAGCATTCTC GAAGAGGTTT TAGAAGCTTT AACATCAGCT
    GGAGAAGAAA GAAAAATCGA CAGATTTTCA TCCATTGTGG AAGGTCTCCG GCATGATTCA
    GTTCAACTGC AAGTAGCGTG TATGCAGCTC ATCAATGCCC TTGTTACATC TCCTGATGAT
    TTGGACTTCA GGCTTCACAT CAGAAATGAA TTTATGCGTT GTGGATTGAA AGAGATCTTG
    CCAGATTTAA AATGTATGAA GAATGATAGC CTGGATATCC AACTTAAAGT CTTTGATGAG
    CATAAGGAAG AAGATTTGCT TGAGTTCTCC CATCGCCTTG AAGATATTAG AGCTGAACTT
    GATGAAGCAT ATGATATTTA CAACATGGTG TGGAACTCAG TTAAGGAAAC TAAAGCAGAG
    GGATATTTCA TTTCTATTCT TCAGCACCTT TTGCTGATTC GAAATGATTA TAGTATAAGG
    CAACAGTACT TCAAATTAAT TGATGAGTGT ATATCTCAGA TTGTATTGCA TAGAGATGGG
    ATGGATCCAG ACTTCACATA TCGGAAAAGA CTGGATTTAG ATTTAAGTCA GTTTGTTGAT
    ACTTGCATAG ATCAAGCAAA ACTAGAAGAG TTTGAAGAGA AAGCGTCAGA ACTGCACAAG
    AAATTTGAAA AAGAGTTTAC CGACCACCAA GAAACTCAGG CTCAATTGCA GAAAAAAGAA
    GCAAAGATTA ATGAACTTCA AGCAGAGTTA CAAGCTTTTA AGTCAGAGTT TGGTGCCTTG
    CCAGCTGGTA GAAATCACTC TTTGCCACAG TCAAAAGAAA ATGGAACTGG CCCCCCAGCA
    CTTGCTCCTC CCCTGCCACT GCCATCTTGT GGAGGCATGC CGCCTCCACC CCCTCCTCCC
    CCACCTCCTC CGCTTCCAGG AATGCCAATG CCTTTTGGTG GTCCCGTGCC TCCTCCACCA
    CCTCCTCTGG GATTTCTCAG TGGGCGAAAC TCTCCTCCTC CACCAACCCT ACCATTTGGG
    TTAAAACCAA AGAAAGAATT TAAACCTGAA ACCAGCATGA GAAGATTGAA TTGGTTAAAG
    ATCAGACCTC ACGAAATGAC TGAAAGCTGT TTCTGGATCA AAGTAAATGA AAATAAGTAT
    GAAAATGTGG ATTTGCTCTG TAAACTTGAG AATACGTTTT GTTGCCAACA AAAAGAGAGA
    AGAGAAGAAG AAGATTTTGA AGAAAAGAAA GCTATTAAGA AAAAAATTAA AGAGCTTAAA
    TTTTTAGATT CTAAAATTGC CCAGAATCTT TCCATCTTCC TGAGCTCTTT TCGGGTGCCA
    TATGAGGAAA TCAAAACGAT GATATTGGAA GTGGATGAAA CACAGTTGGC AGAGTCTATG
    ATTCAGAACC TAATAAAGCA TCTTCCTGAT CAAGAACAAT TAAGCTCACT GTCTCAGTTC
    AAGAATGACT ATAACAACCT ATGTGAACCC GAGCAGTTTG CTGTTGTGAT GAGCAATGTG
    AAGAGACTAC GGCCACGGCT CAGTGCTATT CTCTTTAAGC TTCAGTTTGA AGAGCAGGTG
    AACAACATCA AACCTGACAT CATGGCTGTC AGTACTGCCT GCGAGGAGAT AAAGAAGAGC
    AAAAGCTTCA GCAAGTTGCT GGAACTTGTA TTGCTAATGG GAAACTACAT GAATGCTGGC
    TCCCGGAATG CTCAAACCTT CGGATTTAAC CTTAGCTCTC TCTGTAAACT AAAGGACACG
    AAATCAGCAG ATCAGAAAAC AACACTACTT CATTTTCTGG TGGAAGTATG TGAAGAAAAG
    TATCCTGATA TCCTGAATTT TGTGGATGAT TTGGAACATT TAGACAAAGC TAGTAAAGTC
    TCTGTAGAAA CGCTGGAAAA GAATTTGAAG CAGATGGGAA GGCAGCTTCA ACAGCTTGAG
    AAAGATTTGG AAACCTTTCC CCCTCCTGAG GACTTGCATG ATAAGTTTGT GACAAAGATG
    TCCAGCTTTG TTATCAGCGC AAAAGAACAA TATGAGAAAC TTCTAAAGTT ACATGAAAAT
    ATGGAGAAGT TATACCAGAG TGTGATGGGA TACTATGCCA TTGACGTGAA GAAGATGTCC
    GTGGAAGACT TTTTTAATGA CTTGAATAAT TTCAGAACCA CATTCATGCA AGCAAGAAAG
    GAGAACATCA AAAAAAGAGA AGCAGAGGAA AGGGAAAAAC GTGCCCAAAT AGCCAAAGAA
    TTGGCAGAGA AAGAAAGACT TGAACGCCAA CAAAAGAAAA AGCGCTTGTT AGAAATGAAG
    ACTGAGGGTG ATGAGACAGG AGTGATGGAT AGTCTGCTGG AGGCTTTGCA GTCGGGCGCT
    GCCTTCCGCG ACCGAAGAAA AAGGACGCCA AAGCCAAAAG ATATTCGGCA AAGTCTCAGT
    CCCATGTCCC AGAGGCCTGT TCTGAAAGTT TGTAACCATG AAAATCAGAA AGTGCAGTTG
    ACAGAAGGGT CACGTTCACA CTACAATATC AATTGCAACT CAACGAGGAC TCCAGTCGCC
    AAGGAGCTTA ATTATAATCT AGACACTCAT ACGTCTACAG GGAGGATCAA GGCAGTTGAG
    AAGAAGGAAG CCTGTAATGT AGAAAGCAAC AGAAAAAAGG AAATGGAACT TCTTGGCTCG
    GTTTCTAAAA ACGAATCTGT TCCCGAAGTT GAAGCCCTGC TGGCAAGATT ACGAGCTTTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005633997.1
    CDS133..3675
    Translation

    Target ORF information:

    RefSeq Version XM_005633940.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633940.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CTGGATAAAT TTGCCAGCAT AAGAATTCCA GGGAGCAAGA AGGAGAGACC TCCACTTTCT
    AACCTGAAGA CTGCATTTGC AAGCAATGAT TGCTCTTCAG AGGTGATGGA AAACATTCCA
    AAGTCACTGT CAGAGAATGA AGTCTTAGAA CTTTTCGAAA AGATGATGGA AGATATGAAT
    TTAAATGAAG ATAAGAAGGC ACCATTGCGG GAAAAGGACT TCAGCATCAA AAAAGAAATG
    GTGATGCAGT ACATTAATAC TGCTTCTAAG ACAGGAAGTT TTAAGAGTAA GCGCCAGATC
    TCACCTCAGG AATTCATCCA TGAACTGAAA ATGGGGTCTG CAGATGAGAG ACTTGTAACA
    TGCCTGGAGT CCCTCCGTGT GTCTTTGACT AGTAATCCTG TGAGTTGGGT AGAAAGCTTT
    GGACGCGAGG GGCTTGGATT ATTACTGGAC ATTTTGGAAA GACTGATTAG TGGCAAAATA
    CAAGAGAAAA TTGTAAAGAA GAATCAGCAT AAAGTCATAC AGTGTCTAAA GGCTTTGATG
    AATACACAGT ATGGCTTGGA AAGAATTATG AGTGAAGAGA GGAGTCTTTC TCTATTAGCC
    AAAGCCATCG ATCCTGAGCA CCCAAGTATG ATGACAGATG TGGTGAAACT CCTCTCTGCG
    GTATGCATTG TAGGAGAGGA AAGCATTCTC GAAGAGGTTT TAGAAGCTTT AACATCAGCT
    GGAGAAGAAA GAAAAATCGA CAGATTTTCA TCCATTGTGG AAGGTCTCCG GCATGATTCA
    GTTCAACTGC AAGTAGCGTG TATGCAGCTC ATCAATGCCC TTGTTACATC TCCTGATGAT
    TTGGACTTCA GGCTTCACAT CAGAAATGAA TTTATGCGTT GTGGATTGAA AGAGATCTTG
    CCAGATTTAA AATGTATGAA GAATGATAGC CTGGATATCC AACTTAAAGT CTTTGATGAG
    CATAAGGAAG AAGATTTGCT TGAGTTCTCC CATCGCCTTG AAGATATTAG AGCTGAACTT
    GATGAAGCAT ATGATATTTA CAACATGGTG TGGAACTCAG TTAAGGAAAC TAAAGCAGAG
    GGATATTTCA TTTCTATTCT TCAGCACCTT TTGCTGATTC GAAATGATTA TAGTATAAGG
    CAACAGTACT TCAAATTAAT TGATGAGTGT ATATCTCAGA TTGTATTGCA TAGAGATGGG
    ATGGATCCAG ACTTCACATA TCGGAAAAGA CTGGATTTAG ATTTAAGTCA GTTTGTTGAT
    ACTTGCATAG ATCAAGCAAA ACTAGAAGAG TTTGAAGAGA AAGCGTCAGA ACTGCACAAG
    AAATTTGAAA AAGAGTTTAC CGACCACCAA GAAACTCAGG CTCAATTGCA GAAAAAAGAA
    GCAAAGATTA ATGAACTTCA AGCAGAGTTA CAAGCTTTTA AGTCAGAGTT TGGTGCCTTG
    CCAGCTGGTA GAAATCACTC TTTGCCACAG TCAAAAGAAA ATGGAACTGG CCCCCCAGCA
    CTTGCTCCTC CCCTGCCACT GCCATCTTGT GGAGGCATGC CGCCTCCACC CCCTCCTCCC
    CCACCTCCTC CGCTTCCAGG AATGCCAATG CCTTTTGGTG GTCCCGTGCC TCCTCCACCA
    CCTCCTCTGG GATTTCTCAG TGGGCGAAAC TCTCCTCCTC CACCAACCCT ACCATTTGGG
    TTAAAACCAA AGAAAGAATT TAAACCTGAA ACCAGCATGA GAAGATTGAA TTGGTTAAAG
    ATCAGACCTC ACGAAATGAC TGAAAGCTGT TTCTGGATCA AAGTAAATGA AAATAAGTAT
    GAAAATGTGG ATTTGCTCTG TAAACTTGAG AATACGTTTT GTTGCCAACA AAAAGAGAGA
    AGAGAAGAAG AAGATTTTGA AGAAAAGAAA GCTATTAAGA AAAAAATTAA AGAGCTTAAA
    TTTTTAGATT CTAAAATTGC CCAGAATCTT TCCATCTTCC TGAGCTCTTT TCGGGTGCCA
    TATGAGGAAA TCAAAACGAT GATATTGGAA GTGGATGAAA CACAGTTGGC AGAGTCTATG
    ATTCAGAACC TAATAAAGCA TCTTCCTGAT CAAGAACAAT TAAGCTCACT GTCTCAGTTC
    AAGAATGACT ATAACAACCT ATGTGAACCC GAGCAGTTTG CTGTTGTGAT GAGCAATGTG
    AAGAGACTAC GGCCACGGCT CAGTGCTATT CTCTTTAAGC TTCAGTTTGA AGAGCAGGTG
    AACAACATCA AACCTGACAT CATGGCTGTC AGTACTGCCT GCGAGGAGAT AAAGAAGAGC
    AAAAGCTTCA GCAAGTTGCT GGAACTTGTA TTGCTAATGG GAAACTACAT GAATGCTGGC
    TCCCGGAATG CTCAAACCTT CGGATTTAAC CTTAGCTCTC TCTGTAAACT AAAGGACACG
    AAATCAGCAG ATCAGAAAAC AACACTACTT CATTTTCTGG TGGAAGTATG TGAAGAAAAG
    TATCCTGATA TCCTGAATTT TGTGGATGAT TTGGAACATT TAGACAAAGC TAGTAAAGTC
    TCTGTAGAAA CGCTGGAAAA GAATTTGAAG CAGATGGGAA GGCAGCTTCA ACAGCTTGAG
    AAAGATTTGG AAACCTTTCC CCCTCCTGAG GACTTGCATG ATAAGTTTGT GACAAAGATG
    TCCAGCTTTG TTATCAGCGC AAAAGAACAA TATGAGAAAC TTCTAAAGTT ACATGAAAAT
    ATGGAGAAGT TATACCAGAG TGTGATGGGA TACTATGCCA TTGACGTGAA GAAGATGTCC
    GTGGAAGACT TTTTTAATGA CTTGAATAAT TTCAGAACCA CATTCATGCA AGCAAGAAAG
    GAGAACATCA AAAAAAGAGA AGCAGAGGAA AGGGAAAAAC GTGCCCAAAT AGCCAAAGAA
    TTGGCAGAGA AAGAAAGACT TGAACGCCAA CAAAAGAAAA AGCGCTTGTT AGAAATGAAG
    ACTGAGGGTG ATGAGACAGG AGTGATGGAT AGTCTGCTGG AGGCTTTGCA GTCGGGCGCT
    GCCTTCCGCG ACCGAAGAAA AAGGACGCCA AAGCCAAAAG ATATTCGGCA AAGTCTCAGT
    CCCATGTCCC AGAGGCCTGT TCTGAAAGTT TGTAACCATG AAAATCAGAA AGTGCAGTTG
    ACAGAAGGGT CACGTTCACA CTACAATATC AATTGCAACT CAACGAGGAC TCCAGTCGCC
    AAGGAGCTTA ATTATAATCT AGACACTCAT ACGTCTACAG GGAGGATCAA GGCAGTTGAG
    AAGAAGGAAG CCTGTAATGT AGAAAGCAAC AGAAAAAAGG AAATGGAACT TCTTGGCTCG
    GTTTCTAAAA ACGAATCTGT TCCCGAAGTT GAAGCCCTGC TGGCAAGATT ACGAGCTTTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23590
    Clone ID Related Accession (Same CDS sequence) XM_005633940.2 , XM_005633940.3
    Accession Version XM_005633940.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633940.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CTGGATAAAT TTGCCAGCAT AAGAATTCCA GGGAGCAAGA AGGAGAGACC TCCACTTTCT
    AACCTGAAGA CTGCATTTGC AAGCAATGAT TGCTCTTCAG AGGTGATGGA AAACATTCCA
    AAGTCACTGT CAGAGAATGA AGTCTTAGAA CTTTTCGAAA AGATGATGGA AGATATGAAT
    TTAAATGAAG ATAAGAAGGC ACCATTGCGG GAAAAGGACT TCAGCATCAA AAAAGAAATG
    GTGATGCAGT ACATTAATAC TGCTTCTAAG ACAGGAAGTT TTAAGAGTAA GCGCCAGATC
    TCACCTCAGG AATTCATCCA TGAACTGAAA ATGGGGTCTG CAGATGAGAG ACTTGTAACA
    TGCCTGGAGT CCCTCCGTGT GTCTTTGACT AGTAATCCTG TGAGTTGGGT AGAAAGCTTT
    GGACGCGAGG GGCTTGGATT ATTACTGGAC ATTTTGGAAA GACTGATTAG TGGCAAAATA
    CAAGAGAAAA TTGTAAAGAA GAATCAGCAT AAAGTCATAC AGTGTCTAAA GGCTTTGATG
    AATACACAGT ATGGCTTGGA AAGAATTATG AGTGAAGAGA GGAGTCTTTC TCTATTAGCC
    AAAGCCATCG ATCCTGAGCA CCCAAGTATG ATGACAGATG TGGTGAAACT CCTCTCTGCG
    GTATGCATTG TAGGAGAGGA AAGCATTCTC GAAGAGGTTT TAGAAGCTTT AACATCAGCT
    GGAGAAGAAA GAAAAATCGA CAGATTTTCA TCCATTGTGG AAGGTCTCCG GCATGATTCA
    GTTCAACTGC AAGTAGCGTG TATGCAGCTC ATCAATGCCC TTGTTACATC TCCTGATGAT
    TTGGACTTCA GGCTTCACAT CAGAAATGAA TTTATGCGTT GTGGATTGAA AGAGATCTTG
    CCAGATTTAA AATGTATGAA GAATGATAGC CTGGATATCC AACTTAAAGT CTTTGATGAG
    CATAAGGAAG AAGATTTGCT TGAGTTCTCC CATCGCCTTG AAGATATTAG AGCTGAACTT
    GATGAAGCAT ATGATATTTA CAACATGGTG TGGAACTCAG TTAAGGAAAC TAAAGCAGAG
    GGATATTTCA TTTCTATTCT TCAGCACCTT TTGCTGATTC GAAATGATTA TAGTATAAGG
    CAACAGTACT TCAAATTAAT TGATGAGTGT ATATCTCAGA TTGTATTGCA TAGAGATGGG
    ATGGATCCAG ACTTCACATA TCGGAAAAGA CTGGATTTAG ATTTAAGTCA GTTTGTTGAT
    ACTTGCATAG ATCAAGCAAA ACTAGAAGAG TTTGAAGAGA AAGCGTCAGA ACTGCACAAG
    AAATTTGAAA AAGAGTTTAC CGACCACCAA GAAACTCAGG CTCAATTGCA GAAAAAAGAA
    GCAAAGATTA ATGAACTTCA AGCAGAGTTA CAAGCTTTTA AGTCAGAGTT TGGTGCCTTG
    CCAGCTGGTA GAAATCACTC TTTGCCACAG TCAAAAGAAA ATGGAACTGG CCCCCCAGCA
    CTTGCTCCTC CCCTGCCACT GCCATCTTGT GGAGGCATGC CGCCTCCACC CCCTCCTCCC
    CCACCTCCTC CGCTTCCAGG AATGCCAATG CCTTTTGGTG GTCCCGTGCC TCCTCCACCA
    CCTCCTCTGG GATTTCTCAG TGGGCGAAAC TCTCCTCCTC CACCAACCCT ACCATTTGGG
    TTAAAACCAA AGAAAGAATT TAAACCTGAA ACCAGCATGA GAAGATTGAA TTGGTTAAAG
    ATCAGACCTC ACGAAATGAC TGAAAGCTGT TTCTGGATCA AAGTAAATGA AAATAAGTAT
    GAAAATGTGG ATTTGCTCTG TAAACTTGAG AATACGTTTT GTTGCCAACA AAAAGAGAGA
    AGAGAAGAAG AAGATTTTGA AGAAAAGAAA GCTATTAAGA AAAAAATTAA AGAGCTTAAA
    TTTTTAGATT CTAAAATTGC CCAGAATCTT TCCATCTTCC TGAGCTCTTT TCGGGTGCCA
    TATGAGGAAA TCAAAACGAT GATATTGGAA GTGGATGAAA CACAGTTGGC AGAGTCTATG
    ATTCAGAACC TAATAAAGCA TCTTCCTGAT CAAGAACAAT TAAGCTCACT GTCTCAGTTC
    AAGAATGACT ATAACAACCT ATGTGAACCC GAGCAGTTTG CTGTTGTGAT GAGCAATGTG
    AAGAGACTAC GGCCACGGCT CAGTGCTATT CTCTTTAAGC TTCAGTTTGA AGAGCAGGTG
    AACAACATCA AACCTGACAT CATGGCTGTC AGTACTGCCT GCGAGGAGAT AAAGAAGAGC
    AAAAGCTTCA GCAAGTTGCT GGAACTTGTA TTGCTAATGG GAAACTACAT GAATGCTGGC
    TCCCGGAATG CTCAAACCTT CGGATTTAAC CTTAGCTCTC TCTGTAAACT AAAGGACACG
    AAATCAGCAG ATCAGAAAAC AACACTACTT CATTTTCTGG TGGAAGTATG TGAAGAAAAG
    TATCCTGATA TCCTGAATTT TGTGGATGAT TTGGAACATT TAGACAAAGC TAGTAAAGTC
    TCTGTAGAAA CGCTGGAAAA GAATTTGAAG CAGATGGGAA GGCAGCTTCA ACAGCTTGAG
    AAAGATTTGG AAACCTTTCC CCCTCCTGAG GACTTGCATG ATAAGTTTGT GACAAAGATG
    TCCAGCTTTG TTATCAGCGC AAAAGAACAA TATGAGAAAC TTCTAAAGTT ACATGAAAAT
    ATGGAGAAGT TATACCAGAG TGTGATGGGA TACTATGCCA TTGACGTGAA GAAGATGTCC
    GTGGAAGACT TTTTTAATGA CTTGAATAAT TTCAGAACCA CATTCATGCA AGCAAGAAAG
    GAGAACATCA AAAAAAGAGA AGCAGAGGAA AGGGAAAAAC GTGCCCAAAT AGCCAAAGAA
    TTGGCAGAGA AAGAAAGACT TGAACGCCAA CAAAAGAAAA AGCGCTTGTT AGAAATGAAG
    ACTGAGGGTG ATGAGACAGG AGTGATGGAT AGTCTGCTGG AGGCTTTGCA GTCGGGCGCT
    GCCTTCCGCG ACCGAAGAAA AAGGACGCCA AAGCCAAAAG ATATTCGGCA AAGTCTCAGT
    CCCATGTCCC AGAGGCCTGT TCTGAAAGTT TGTAACCATG AAAATCAGAA AGTGCAGTTG
    ACAGAAGGGT CACGTTCACA CTACAATATC AATTGCAACT CAACGAGGAC TCCAGTCGCC
    AAGGAGCTTA ATTATAATCT AGACACTCAT ACGTCTACAG GGAGGATCAA GGCAGTTGAG
    AAGAAGGAAG CCTGTAATGT AGAAAGCAAC AGAAAAAAGG AAATGGAACT TCTTGGCTCG
    GTTTCTAAAA ACGAATCTGT TCCCGAAGTT GAAGCCCTGC TGGCAAGATT ACGAGCTTTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005633997.1
    CDS16..3558
    Translation

    Target ORF information:

    RefSeq Version XM_005633940.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633940.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CTGGATAAAT TTGCCAGCAT AAGAATTCCA GGGAGCAAGA AGGAGAGACC TCCACTTTCT
    AACCTGAAGA CTGCATTTGC AAGCAATGAT TGCTCTTCAG AGGTGATGGA AAACATTCCA
    AAGTCACTGT CAGAGAATGA AGTCTTAGAA CTTTTCGAAA AGATGATGGA AGATATGAAT
    TTAAATGAAG ATAAGAAGGC ACCATTGCGG GAAAAGGACT TCAGCATCAA AAAAGAAATG
    GTGATGCAGT ACATTAATAC TGCTTCTAAG ACAGGAAGTT TTAAGAGTAA GCGCCAGATC
    TCACCTCAGG AATTCATCCA TGAACTGAAA ATGGGGTCTG CAGATGAGAG ACTTGTAACA
    TGCCTGGAGT CCCTCCGTGT GTCTTTGACT AGTAATCCTG TGAGTTGGGT AGAAAGCTTT
    GGACGCGAGG GGCTTGGATT ATTACTGGAC ATTTTGGAAA GACTGATTAG TGGCAAAATA
    CAAGAGAAAA TTGTAAAGAA GAATCAGCAT AAAGTCATAC AGTGTCTAAA GGCTTTGATG
    AATACACAGT ATGGCTTGGA AAGAATTATG AGTGAAGAGA GGAGTCTTTC TCTATTAGCC
    AAAGCCATCG ATCCTGAGCA CCCAAGTATG ATGACAGATG TGGTGAAACT CCTCTCTGCG
    GTATGCATTG TAGGAGAGGA AAGCATTCTC GAAGAGGTTT TAGAAGCTTT AACATCAGCT
    GGAGAAGAAA GAAAAATCGA CAGATTTTCA TCCATTGTGG AAGGTCTCCG GCATGATTCA
    GTTCAACTGC AAGTAGCGTG TATGCAGCTC ATCAATGCCC TTGTTACATC TCCTGATGAT
    TTGGACTTCA GGCTTCACAT CAGAAATGAA TTTATGCGTT GTGGATTGAA AGAGATCTTG
    CCAGATTTAA AATGTATGAA GAATGATAGC CTGGATATCC AACTTAAAGT CTTTGATGAG
    CATAAGGAAG AAGATTTGCT TGAGTTCTCC CATCGCCTTG AAGATATTAG AGCTGAACTT
    GATGAAGCAT ATGATATTTA CAACATGGTG TGGAACTCAG TTAAGGAAAC TAAAGCAGAG
    GGATATTTCA TTTCTATTCT TCAGCACCTT TTGCTGATTC GAAATGATTA TAGTATAAGG
    CAACAGTACT TCAAATTAAT TGATGAGTGT ATATCTCAGA TTGTATTGCA TAGAGATGGG
    ATGGATCCAG ACTTCACATA TCGGAAAAGA CTGGATTTAG ATTTAAGTCA GTTTGTTGAT
    ACTTGCATAG ATCAAGCAAA ACTAGAAGAG TTTGAAGAGA AAGCGTCAGA ACTGCACAAG
    AAATTTGAAA AAGAGTTTAC CGACCACCAA GAAACTCAGG CTCAATTGCA GAAAAAAGAA
    GCAAAGATTA ATGAACTTCA AGCAGAGTTA CAAGCTTTTA AGTCAGAGTT TGGTGCCTTG
    CCAGCTGGTA GAAATCACTC TTTGCCACAG TCAAAAGAAA ATGGAACTGG CCCCCCAGCA
    CTTGCTCCTC CCCTGCCACT GCCATCTTGT GGAGGCATGC CGCCTCCACC CCCTCCTCCC
    CCACCTCCTC CGCTTCCAGG AATGCCAATG CCTTTTGGTG GTCCCGTGCC TCCTCCACCA
    CCTCCTCTGG GATTTCTCAG TGGGCGAAAC TCTCCTCCTC CACCAACCCT ACCATTTGGG
    TTAAAACCAA AGAAAGAATT TAAACCTGAA ACCAGCATGA GAAGATTGAA TTGGTTAAAG
    ATCAGACCTC ACGAAATGAC TGAAAGCTGT TTCTGGATCA AAGTAAATGA AAATAAGTAT
    GAAAATGTGG ATTTGCTCTG TAAACTTGAG AATACGTTTT GTTGCCAACA AAAAGAGAGA
    AGAGAAGAAG AAGATTTTGA AGAAAAGAAA GCTATTAAGA AAAAAATTAA AGAGCTTAAA
    TTTTTAGATT CTAAAATTGC CCAGAATCTT TCCATCTTCC TGAGCTCTTT TCGGGTGCCA
    TATGAGGAAA TCAAAACGAT GATATTGGAA GTGGATGAAA CACAGTTGGC AGAGTCTATG
    ATTCAGAACC TAATAAAGCA TCTTCCTGAT CAAGAACAAT TAAGCTCACT GTCTCAGTTC
    AAGAATGACT ATAACAACCT ATGTGAACCC GAGCAGTTTG CTGTTGTGAT GAGCAATGTG
    AAGAGACTAC GGCCACGGCT CAGTGCTATT CTCTTTAAGC TTCAGTTTGA AGAGCAGGTG
    AACAACATCA AACCTGACAT CATGGCTGTC AGTACTGCCT GCGAGGAGAT AAAGAAGAGC
    AAAAGCTTCA GCAAGTTGCT GGAACTTGTA TTGCTAATGG GAAACTACAT GAATGCTGGC
    TCCCGGAATG CTCAAACCTT CGGATTTAAC CTTAGCTCTC TCTGTAAACT AAAGGACACG
    AAATCAGCAG ATCAGAAAAC AACACTACTT CATTTTCTGG TGGAAGTATG TGAAGAAAAG
    TATCCTGATA TCCTGAATTT TGTGGATGAT TTGGAACATT TAGACAAAGC TAGTAAAGTC
    TCTGTAGAAA CGCTGGAAAA GAATTTGAAG CAGATGGGAA GGCAGCTTCA ACAGCTTGAG
    AAAGATTTGG AAACCTTTCC CCCTCCTGAG GACTTGCATG ATAAGTTTGT GACAAAGATG
    TCCAGCTTTG TTATCAGCGC AAAAGAACAA TATGAGAAAC TTCTAAAGTT ACATGAAAAT
    ATGGAGAAGT TATACCAGAG TGTGATGGGA TACTATGCCA TTGACGTGAA GAAGATGTCC
    GTGGAAGACT TTTTTAATGA CTTGAATAAT TTCAGAACCA CATTCATGCA AGCAAGAAAG
    GAGAACATCA AAAAAAGAGA AGCAGAGGAA AGGGAAAAAC GTGCCCAAAT AGCCAAAGAA
    TTGGCAGAGA AAGAAAGACT TGAACGCCAA CAAAAGAAAA AGCGCTTGTT AGAAATGAAG
    ACTGAGGGTG ATGAGACAGG AGTGATGGAT AGTCTGCTGG AGGCTTTGCA GTCGGGCGCT
    GCCTTCCGCG ACCGAAGAAA AAGGACGCCA AAGCCAAAAG ATATTCGGCA AAGTCTCAGT
    CCCATGTCCC AGAGGCCTGT TCTGAAAGTT TGTAACCATG AAAATCAGAA AGTGCAGTTG
    ACAGAAGGGT CACGTTCACA CTACAATATC AATTGCAACT CAACGAGGAC TCCAGTCGCC
    AAGGAGCTTA ATTATAATCT AGACACTCAT ACGTCTACAG GGAGGATCAA GGCAGTTGAG
    AAGAAGGAAG CCTGTAATGT AGAAAGCAAC AGAAAAAAGG AAATGGAACT TCTTGGCTCG
    GTTTCTAAAA ACGAATCTGT TCCCGAAGTT GAAGCCCTGC TGGCAAGATT ACGAGCTTTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23593
    Clone ID Related Accession (Same CDS sequence) XM_005633943.1
    Accession Version XM_005633943.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein diaphanous homolog 3 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGCCAACTA CTTCTACAAT AGAGAGTACT CCCTTCAGCA AAAAGAGCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634000.1
    CDS133..3495
    Translation

    Target ORF information:

    RefSeq Version XM_005633943.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633943.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGCCAACTA CTTCTACAAT AGAGAGTACT CCCTTCAGCA AAAAGAGCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23595
    Clone ID Related Accession (Same CDS sequence) XM_005633945.2 , XM_005633945.3
    Accession Version XM_005633945.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein diaphanous homolog 3 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGGGATGGA GAAAGAGCTG ACATTGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634002.1
    CDS133..3474
    Translation

    Target ORF information:

    RefSeq Version XM_005633945.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diaphanous-related formin 3 (DIAPH3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633945.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGGGATGGA GAAAGAGCTG ACATTGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23595
    Clone ID Related Accession (Same CDS sequence) XM_005633945.2 , XM_005633945.3
    Accession Version XM_005633945.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633945.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGGGATGGA GAAAGAGCTG ACATTGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634002.1
    CDS16..3357
    Translation

    Target ORF information:

    RefSeq Version XM_005633945.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633945.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGGGATGGA GAAAGAGCTG ACATTGTGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe296160
    Clone ID Related Accession (Same CDS sequence) XM_022408007.1
    Accession Version XM_022408007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGCATTTTT GAACACCAGC CACATGCAGA ACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022263715.1
    CDS16..3363
    Translation

    Target ORF information:

    RefSeq Version XM_022408007.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022408007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGCATTTTT GAACACCAGC CACATGCAGA ACCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe296161
    Clone ID Related Accession (Same CDS sequence) XM_022408008.1
    Accession Version XM_022408008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGGTTGTAT ACATCTGTAT CACGGCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022263716.1
    CDS16..3357
    Translation

    Target ORF information:

    RefSeq Version XM_022408008.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022408008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGGTTGTAT ACATCTGTAT CACGGCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe296162
    Clone ID Related Accession (Same CDS sequence) XM_022408009.1
    Accession Version XM_022408009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGATACAAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022263717.1
    CDS16..3339
    Translation

    Target ORF information:

    RefSeq Version XM_022408009.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022408009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GCAAGCAAGA AAGGAGAACA TCAAAAAAAG AGAAGCAGAG
    GAAAGGGAAA AACGTGCCCA AATAGCCAAA GAATTGGCAG AGAAAGAAAG ACTTGAACGC
    CAACAAAAGA AAAAGCGCTT GTTAGAAATG AAGACTGAGG GTGATGAGAC AGGAGTGATG
    GATAGTCTGC TGGAGGCTTT GCAGTCGGGC GCTGCCTTCC GCGACCGAAG AAAAAGGACG
    CCAAAGCCAA AAGATATTCG GCAAAGTCTC AGTCCCATGT CCCAGAGGCC TGTTCTGAAA
    GTTTGTAACC ATGATACAAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe296163
    Clone ID Related Accession (Same CDS sequence) XM_022408010.1
    Accession Version XM_022408010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein diaphanous homolog 3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GATGTTTGTG GGGCTTAAAA GTTTCGAAGG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022263718.1
    CDS16..3069
    Translation

    Target ORF information:

    RefSeq Version XM_022408010.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diaphanous related formin 3 (DIAPH3), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022408010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATCGGC CCCGGCCGCG GCCGCACCAC CCGAAGCCCG GCTCCGCCGC CGCGGGGGCG 
    CCCTACCCCG CCTCCGCCTC GCTCCGCAGC TGCAGGGAAA GCAAGATGCC GCGCAGGAAG
    GGCCCCCAGC GCCCTCCGCC CGGCGCCGTC GAGGAGCCTG GAGAGAAACG CCCCAAGTTC
    CATTTAAATA TTAGGACATT GACGGATGAT ATGCTGGATA AATTTGCCAG CATAAGAATT
    CCAGGGAGCA AGAAGGAGAG ACCTCCACTT TCTAACCTGA AGACTGCATT TGCAAGCAAT
    GATTGCTCTT CAGAGGTGAT GGAAAACATT CCAAAGTCAC TGTCAGAGAA TGAAGTCTTA
    GAACTTTTCG AAAAGATGAT GGAAGATATG AATTTAAATG AAGATAAGAA GGCACCATTG
    CGGGAAAAGG ACTTCAGCAT CAAAAAAGAA ATGGTGATGC AGTACATTAA TACTGCTTCT
    AAGACAGGAA GTTTTAAGAG TAAGCGCCAG ATCTCACCTC AGGAATTCAT CCATGAACTG
    AAAATGGGGT CTGCAGATGA GAGACTTGTA ACATGCCTGG AGTCCCTCCG TGTGTCTTTG
    ACTAGTAATC CTGTGAGTTG GGTAGAAAGC TTTGGACGCG AGGGGCTTGG ATTATTACTG
    GACATTTTGG AAAGACTGAT TAGTGGCAAA ATACAAGAGA AAATTGTAAA GAAGAATCAG
    CATAAAGTCA TACAGTGTCT AAAGGCTTTG ATGAATACAC AGTATGGCTT GGAAAGAATT
    ATGAGTGAAG AGAGGAGTCT TTCTCTATTA GCCAAAGCCA TCGATCCTGA GCACCCAAGT
    ATGATGACAG ATGTGGTGAA ACTCCTCTCT GCGGTATGCA TTGTAGGAGA GGAAAGCATT
    CTCGAAGAGG TTTTAGAAGC TTTAACATCA GCTGGAGAAG AAAGAAAAAT CGACAGATTT
    TCATCCATTG TGGAAGGTCT CCGGCATGAT TCAGTTCAAC TGCAAGTAGC GTGTATGCAG
    CTCATCAATG CCCTTGTTAC ATCTCCTGAT GATTTGGACT TCAGGCTTCA CATCAGAAAT
    GAATTTATGC GTTGTGGATT GAAAGAGATC TTGCCAGATT TAAAATGTAT GAAGAATGAT
    AGCCTGGATA TCCAACTTAA AGTCTTTGAT GAGCATAAGG AAGAAGATTT GCTTGAGTTC
    TCCCATCGCC TTGAAGATAT TAGAGCTGAA CTTGATGAAG CATATGATAT TTACAACATG
    GTGTGGAACT CAGTTAAGGA AACTAAAGCA GAGGGATATT TCATTTCTAT TCTTCAGCAC
    CTTTTGCTGA TTCGAAATGA TTATAGTATA AGGCAACAGT ACTTCAAATT AATTGATGAG
    TGTATATCTC AGATTGTATT GCATAGAGAT GGGATGGATC CAGACTTCAC ATATCGGAAA
    AGACTGGATT TAGATTTAAG TCAGTTTGTT GATACTTGCA TAGATCAAGC AAAACTAGAA
    GAGTTTGAAG AGAAAGCGTC AGAACTGCAC AAGAAATTTG AAAAAGAGTT TACCGACCAC
    CAAGAAACTC AGGCTCAATT GCAGAAAAAA GAAGCAAAGA TTAATGAACT TCAAGCAGAG
    TTACAAGCTT TTAAGTCAGA GTTTGGTGCC TTGCCAGCTG GTAGAAATCA CTCTTTGCCA
    CAGTCAAAAG AAAATGGAAC TGGCCCCCCA GCACTTGCTC CTCCCCTGCC ACTGCCATCT
    TGTGGAGGCA TGCCGCCTCC ACCCCCTCCT CCCCCACCTC CTCCGCTTCC AGGAATGCCA
    ATGCCTTTTG GTGGTCCCGT GCCTCCTCCA CCACCTCCTC TGGGATTTCT CAGTGGGCGA
    AACTCTCCTC CTCCACCAAC CCTACCATTT GGGTTAAAAC CAAAGAAAGA ATTTAAACCT
    GAAACCAGCA TGAGAAGATT GAATTGGTTA AAGATCAGAC CTCACGAAAT GACTGAAAGC
    TGTTTCTGGA TCAAAGTAAA TGAAAATAAG TATGAAAATG TGGATTTGCT CTGTAAACTT
    GAGAATACGT TTTGTTGCCA ACAAAAAGAG AGAAGAGAAG AAGAAGATTT TGAAGAAAAG
    AAAGCTATTA AGAAAAAAAT TAAAGAGCTT AAATTTTTAG ATTCTAAAAT TGCCCAGAAT
    CTTTCCATCT TCCTGAGCTC TTTTCGGGTG CCATATGAGG AAATCAAAAC GATGATATTG
    GAAGTGGATG AAACACAGTT GGCAGAGTCT ATGATTCAGA ACCTAATAAA GCATCTTCCT
    GATCAAGAAC AATTAAGCTC ACTGTCTCAG TTCAAGAATG ACTATAACAA CCTATGTGAA
    CCCGAGCAGT TTGCTGTTGT GATGAGCAAT GTGAAGAGAC TACGGCCACG GCTCAGTGCT
    ATTCTCTTTA AGCTTCAGTT TGAAGAGCAG GTGAACAACA TCAAACCTGA CATCATGGCT
    GTCAGTACTG CCTGCGAGGA GATAAAGAAG AGCAAAAGCT TCAGCAAGTT GCTGGAACTT
    GTATTGCTAA TGGGAAACTA CATGAATGCT GGCTCCCGGA ATGCTCAAAC CTTCGGATTT
    AACCTTAGCT CTCTCTGTAA ACTAAAGGAC ACGAAATCAG CAGATCAGAA AACAACACTA
    CTTCATTTTC TGGTGGAAGT ATGTGAAGAA AAGTATCCTG ATATCCTGAA TTTTGTGGAT
    GATTTGGAAC ATTTAGACAA AGCTAGTAAA GTCTCTGTAG AAACGCTGGA AAAGAATTTG
    AAGCAGATGG GAAGGCAGCT TCAACAGCTT GAGAAAGATT TGGAAACCTT TCCCCCTCCT
    GAGGACTTGC ATGATAAGTT TGTGACAAAG ATGTCCAGCT TTGTTATCAG CGCAAAAGAA
    CAATATGAGA AACTTCTAAA GTTACATGAA AATATGGAGA AGTTATACCA GAGTGTGATG
    GGATACTATG CCATTGACGT GAAGAAGATG TCCGTGGAAG ACTTTTTTAA TGACTTGAAT
    AATTTCAGAA CCACATTCAT GATGTTTGTG GGGCTTAAAA GTTTCGAAGG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.