TRIM66 cDNA ORF clone, Canis lupus familiaris(dog)

The following TRIM66 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIM66 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe296054 XM_022407811.1
Latest version!
Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe42226 XM_014122779.2
Latest version!
Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe42227 XM_014122781.1
Latest version!
Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42227 XM_022407812.1
Latest version!
Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42227 XM_014122780.2
Latest version!
Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42227 XM_014122782.2
Latest version!
Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42227 XM_014122783.2
Latest version!
Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42227 XM_014122784.1
Latest version!
Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe23168 XM_005633636.3
Latest version!
Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe23169 XM_005633637.3
Latest version!
Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe42226 XM_014122779.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe42227 XM_014122783.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42227 XM_014122780.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42227 XM_014122782.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe23168 XM_005633636.2 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe23169 XM_005633637.2 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe296054
    Clone ID Related Accession (Same CDS sequence) XM_022407811.1
    Accession Version XM_022407811.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tripartite motif-containing protein 66 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
    CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA
    AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC
    AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA
    AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC
    TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC
    TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG
    CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG
    AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG
    GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA
    CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA
    GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG
    GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT
    CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG
    CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC
    AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG
    GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC
    ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC
    AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATGC AGCGATTGCT GGAGGCAAAC
    TGTAACACAG ATCCGGGCTC CCCCTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG
    ACCAAGCAGC TGGCTTCCCT TGGCTGTATA ACTACTGAAG GTGGACAGCT GTCCCGGGCA
    GATACTCCTG CTTATGGAGG CTTACAGGGA CCATCATCCT TTTATCAAAG CCACCAGTCC
    CCAGTGGCTC AGCAAGAGAC TCTTAGCCAC CCCTCACATA AGTTCCAGTC TCCAGCACTG
    TGTTCTTCAT CTGTATGCTG CTCCCACTGC TCCCCAGCCT CGCCTTCCCT CAAAGGCCAG
    GTCCCCCCAC CCAGCATACA CTCAGCCCAC AGCTTTAGGC AGCCCTCAGA GATGGCACCC
    CAACAGCTAG GCTCTCTGCA GTGCTCTACT CTGCTGCCCA GGGAGAAGGA GCTGCCTTGC
    AATCCTCAGC CACCAAAACT GATGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG
    GAGAACACAT CCCAGCGGCT GGGGCAACAG CCAACTTCCC AGCCAGTATG CATTGTGCCC
    CCGCAGGATG TTCAGCAAGG AGCCCATGCC CAACCCACCT TACAGACACC CTCTATCCAA
    GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGCCACT TTCAGCAGCA GCCACAGCAG
    CAACTGCCAC CTCCGCCACC TCTGCCACCC CCACCCCAGC AGCAGCCACT GCCACCTCTA
    CCTCCACCCC AGCATCCAGC TTCTAGTCAG CATGGGAGCC CTCCTGGGCC TGCCTGTTCC
    CAGAACATGG ATATAATGCA CCACAAATTT GAGCTGGAGG AGATGCAGAA GGACCTGGAG
    CTTCTTCTTC AGGCTCAACA GCCCAGCCTA CAGCTGAATC AGACCAAATC TCCTCAGCAT
    CTTCAGCAAA CCATTGTGGG ACAGATCAAC TACATTGTGA GGCAGCCAGC ACCCGTCCAG
    TCCCAGAGCC AAGAGGATGC TGCACAGGCT TCGGATGAGC ACCCCACATC TGAAGGTCCA
    AAGCCGCCTC TCCCACTTGA CAAGAATACT GCTGCTAACT TGCCCCAGGC GTCTGGGGAG
    GAAACCCCTC CTGGTGTCCC CCCACCAGAC GGCACCATCC CGCACTCCTC TCCAAGTGTG
    GTGAGAAAGC ACTCCACTTC AGTGAGCATC ATGGGCTTCT CCAACACGCT AGAGATGGAG
    CTGTCATCTA CCAGGCTAGC CAGGACCCTG GAGCCGCAGA TCCAGAGCGT GAGCAGCCTG
    ACTGCTGCCC CTTCCCACGT GGTCCCAAGC CTGCTGAGCG GCACCCCCAC AACTGTGTCC
    AGCCTGCTGA GCGGTCAGAG CCAGGCTGTG CCCAGCCTGA CAGTTAGTCC TCTGCAGACC
    ATACCCAGTC TTGTACGTGG CACACCCCGG ACCGTGCCCG GTTTGATGAG TGATTCCTCC
    CAGGCCATCA CAAGCCTAGC AAGTGATCAC TCACAGGCCA GGCCCAAGCT GATGTCTGGT
    CCCACCCAGA CTCTGCCAAG TCTGGCTACT TGTCCACTGC AGAGCATGCC TCCGGCTTCT
    GACACGCAAC CGGAAACTGC ATCCAGTGGC AGTCCTGGCT CTGGCGGAGC TGTGGGGAGC
    CTGTGCCCTG GAGATGGGGC TGACCCCTCT CTTGGGAATG CCCTGTGTAA GATGGAAAGT
    GAGGATTCTT CCCGCTTCAC TGACTCTCTG GAACAGGACC CCACAACTCC TGGTCTGGAG
    GTTTCCAAGG ATTTGGCTAT CCCTTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG
    AAACCTCCAG TGGTCCCAGC AGTCAGCACA GCTATGGCTC TGCAGCAGTA CCAGAACCCA
    AAAGAGTATG AGAATTTTGA AGAAGCCCTG GAGCTGAATG TAAAAGAGAA CCAGAGTATC
    AGGCGAGAGC CTAAGATTCC CTATGTGCGG CTGGAGCGGC TTAAGATCTG TGCTGCCTCC
    TCGGGAGAGA TGCCTGTGTT CAAGCTGAAG CCCCAGAAGA ATGAGCAGGA TGGGAGCTTC
    CTGCTGATCA TCGAGTGTGG CACAGAGTCC TCCAGCATGT CCATTAAGGT CAGCCAGAAC
    AACCTGCCTG ATGCCATCCA GGCCCCAAGT CTGGGGGGAA GAAAGGTCAC TGTCACTTCT
    CTGGCTGGAC AACAGCCACC AGAAGGGGAG GGTGCATCTC CTGAAGAACA AAGGCTCATC
    CCTCGGACCC TTGGAGCCAA GAAGGGGCCC CCAGTACCAA TAGAGAATGA GGACTTCTGT
    GCTGTGTGCC TCAATGGGGG CGAGCTACTG TGCTGTGACC GCTGCCCTAA GGTGTTCCAC
    CTCTCCTGCC ACCTGCCGGC CTTGCTTGGC TTCCCAGGGG GAGATTGGGT GTGTACCTTG
    TGCCGCAGCC TGACCCAGCC CGAGATGGAG TATGACTGTG AGAATGCCCG CTATTACCAA
    CCTGGGATGC GGGACACCCC TGGCCTGAGC ATCTATGACC AAAAGAAGTG TGAAAAGTTG
    GTTCTGTCCT TGTGCTGCAA TAGCCTCAGC CTGCCCTTCC ACGAGCCTGT CAGCCCGCTG
    GCCCGGCATT ACTACCAGAT TATCAAGAGG CCCATGGACC TGTCAATCAT CCGGAGGAAG
    CTGCAAAAGA AGGACCCAGC TCACTACACC ACCCCAGAGG AGGTGGTGTC AGACGTCCGC
    CTCATGTTCT GGAACTGTGC TAAATTCAAT TATCCTGACT CAGAAGTTGC AGAGGCTGGC
    CGCTGCCTGG AAGTTTTCTT TGAGGGCTGG TTGAAGGAGA TCTATCCAGA AAAACAGTTT
    GCCCAGCCGA GGCAGGAGGA CTCAGACTCC GAGGAGGTGT CTAGCGAGAG TGGCTATTCC
    ACTCCCCAGG GCTTCCCATG GCCTCCCTAT GTGCAGGAGG GCATCCAACC CAAGAGGCGG
    CGGCGACATA TGGAGAATGA AAGAGCAAAA AGAATGTCAT TCCGCTTGGC CAGCAGCGTC
    TCCCAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022263519.1
    CDS94..4245
    Translation

    Target ORF information:

    RefSeq Version XM_022407811.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022407811.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
    CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA
    AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC
    AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA
    AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC
    TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC
    TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG
    CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG
    AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG
    GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA
    CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA
    GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG
    GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT
    CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG
    CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC
    AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG
    GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC
    ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC
    AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATGC AGCGATTGCT GGAGGCAAAC
    TGTAACACAG ATCCGGGCTC CCCCTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG
    ACCAAGCAGC TGGCTTCCCT TGGCTGTATA ACTACTGAAG GTGGACAGCT GTCCCGGGCA
    GATACTCCTG CTTATGGAGG CTTACAGGGA CCATCATCCT TTTATCAAAG CCACCAGTCC
    CCAGTGGCTC AGCAAGAGAC TCTTAGCCAC CCCTCACATA AGTTCCAGTC TCCAGCACTG
    TGTTCTTCAT CTGTATGCTG CTCCCACTGC TCCCCAGCCT CGCCTTCCCT CAAAGGCCAG
    GTCCCCCCAC CCAGCATACA CTCAGCCCAC AGCTTTAGGC AGCCCTCAGA GATGGCACCC
    CAACAGCTAG GCTCTCTGCA GTGCTCTACT CTGCTGCCCA GGGAGAAGGA GCTGCCTTGC
    AATCCTCAGC CACCAAAACT GATGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG
    GAGAACACAT CCCAGCGGCT GGGGCAACAG CCAACTTCCC AGCCAGTATG CATTGTGCCC
    CCGCAGGATG TTCAGCAAGG AGCCCATGCC CAACCCACCT TACAGACACC CTCTATCCAA
    GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGCCACT TTCAGCAGCA GCCACAGCAG
    CAACTGCCAC CTCCGCCACC TCTGCCACCC CCACCCCAGC AGCAGCCACT GCCACCTCTA
    CCTCCACCCC AGCATCCAGC TTCTAGTCAG CATGGGAGCC CTCCTGGGCC TGCCTGTTCC
    CAGAACATGG ATATAATGCA CCACAAATTT GAGCTGGAGG AGATGCAGAA GGACCTGGAG
    CTTCTTCTTC AGGCTCAACA GCCCAGCCTA CAGCTGAATC AGACCAAATC TCCTCAGCAT
    CTTCAGCAAA CCATTGTGGG ACAGATCAAC TACATTGTGA GGCAGCCAGC ACCCGTCCAG
    TCCCAGAGCC AAGAGGATGC TGCACAGGCT TCGGATGAGC ACCCCACATC TGAAGGTCCA
    AAGCCGCCTC TCCCACTTGA CAAGAATACT GCTGCTAACT TGCCCCAGGC GTCTGGGGAG
    GAAACCCCTC CTGGTGTCCC CCCACCAGAC GGCACCATCC CGCACTCCTC TCCAAGTGTG
    GTGAGAAAGC ACTCCACTTC AGTGAGCATC ATGGGCTTCT CCAACACGCT AGAGATGGAG
    CTGTCATCTA CCAGGCTAGC CAGGACCCTG GAGCCGCAGA TCCAGAGCGT GAGCAGCCTG
    ACTGCTGCCC CTTCCCACGT GGTCCCAAGC CTGCTGAGCG GCACCCCCAC AACTGTGTCC
    AGCCTGCTGA GCGGTCAGAG CCAGGCTGTG CCCAGCCTGA CAGTTAGTCC TCTGCAGACC
    ATACCCAGTC TTGTACGTGG CACACCCCGG ACCGTGCCCG GTTTGATGAG TGATTCCTCC
    CAGGCCATCA CAAGCCTAGC AAGTGATCAC TCACAGGCCA GGCCCAAGCT GATGTCTGGT
    CCCACCCAGA CTCTGCCAAG TCTGGCTACT TGTCCACTGC AGAGCATGCC TCCGGCTTCT
    GACACGCAAC CGGAAACTGC ATCCAGTGGC AGTCCTGGCT CTGGCGGAGC TGTGGGGAGC
    CTGTGCCCTG GAGATGGGGC TGACCCCTCT CTTGGGAATG CCCTGTGTAA GATGGAAAGT
    GAGGATTCTT CCCGCTTCAC TGACTCTCTG GAACAGGACC CCACAACTCC TGGTCTGGAG
    GTTTCCAAGG ATTTGGCTAT CCCTTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG
    AAACCTCCAG TGGTCCCAGC AGTCAGCACA GCTATGGCTC TGCAGCAGTA CCAGAACCCA
    AAAGAGTATG AGAATTTTGA AGAAGCCCTG GAGCTGAATG TAAAAGAGAA CCAGAGTATC
    AGGCGAGAGC CTAAGATTCC CTATGTGCGG CTGGAGCGGC TTAAGATCTG TGCTGCCTCC
    TCGGGAGAGA TGCCTGTGTT CAAGCTGAAG CCCCAGAAGA ATGAGCAGGA TGGGAGCTTC
    CTGCTGATCA TCGAGTGTGG CACAGAGTCC TCCAGCATGT CCATTAAGGT CAGCCAGAAC
    AACCTGCCTG ATGCCATCCA GGCCCCAAGT CTGGGGGGAA GAAAGGTCAC TGTCACTTCT
    CTGGCTGGAC AACAGCCACC AGAAGGGGAG GGTGCATCTC CTGAAGAACA AAGGCTCATC
    CCTCGGACCC TTGGAGCCAA GAAGGGGCCC CCAGTACCAA TAGAGAATGA GGACTTCTGT
    GCTGTGTGCC TCAATGGGGG CGAGCTACTG TGCTGTGACC GCTGCCCTAA GGTGTTCCAC
    CTCTCCTGCC ACCTGCCGGC CTTGCTTGGC TTCCCAGGGG GAGATTGGGT GTGTACCTTG
    TGCCGCAGCC TGACCCAGCC CGAGATGGAG TATGACTGTG AGAATGCCCG CTATTACCAA
    CCTGGGATGC GGGACACCCC TGGCCTGAGC ATCTATGACC AAAAGAAGTG TGAAAAGTTG
    GTTCTGTCCT TGTGCTGCAA TAGCCTCAGC CTGCCCTTCC ACGAGCCTGT CAGCCCGCTG
    GCCCGGCATT ACTACCAGAT TATCAAGAGG CCCATGGACC TGTCAATCAT CCGGAGGAAG
    CTGCAAAAGA AGGACCCAGC TCACTACACC ACCCCAGAGG AGGTGGTGTC AGACGTCCGC
    CTCATGTTCT GGAACTGTGC TAAATTCAAT TATCCTGACT CAGAAGTTGC AGAGGCTGGC
    CGCTGCCTGG AAGTTTTCTT TGAGGGCTGG TTGAAGGAGA TCTATCCAGA AAAACAGTTT
    GCCCAGCCGA GGCAGGAGGA CTCAGACTCC GAGGAGGTGT CTAGCGAGAG TGGCTATTCC
    ACTCCCCAGG GCTTCCCATG GCCTCCCTAT GTGCAGGAGG GCATCCAACC CAAGAGGCGG
    CGGCGACATA TGGAGAATGA AAGAGCAAAA AGAATGTCAT TCCGCTTGGC CAGCAGCGTC
    TCCCAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42226
    Clone ID Related Accession (Same CDS sequence) XM_014122779.1 , XM_014122779.2
    Accession Version XM_014122779.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tripartite motif-containing protein 66 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGGAG TGGAGCTTGC TCGATCTACA CGCTGCTGCA CACTTCAGGA TATCATGGGA 
    AGAGCCCCAG TCCCGGTCAC AAGCACAGCC ACGGATACGG GCTTGAGATT TATGGGGCTG
    CCACTAGTTG ACCAGAAACA CAGGAATGGT CAGATGGAGG CCATGATATT GACCTGCTCA
    TTGTGCCAAC AAGACCTGCA AAATGTGGGC GCCCGGTTCC TACCCTGCCA GCATTTGCTC
    TGCAAGAACT GCTTCCAGGG CTTCATCCAG GAGCTGAGAC AGGTTTCCAA AGCTCACAGG
    ACCGTTCCAC CTGAGCTCAT CTCCTGTCCT GGGTGTAAGC GAGTATATCT TACCAGGGAT
    GTAAATGAAC ATTTTTTTCT GCAGTGCTTT TCTACAGGGC GATCCAAGAT GGCCAGGAAC
    TGCTCCGAGT GCAAGGAGAA GAGGGCAGCG CATATCCTGT GCACCTACTG CAACCGCTGG
    CTGTGCAGCT CATGCACAGA GGAGCACCGG CATGGCCCCG CCCCTGGGGG CCCACTCTTT
    CCGCGGGCCC ACAAGGGACC ACCAGGAGTG AGCAGTGGTT CTGGGGACTT CGCCTTGTAC
    TGTCCTCTAC ACACACAGGA AGTGCTCAAG CTATTCTGCG AGACCTGTGA TGTGCTCACG
    TGCCATAGCT GCCTCATGGT GGAACACAAA GAACACAGGT GTAGACATCT TGAAGAAGTT
    TTGCAAAACC AGAGGATGCT TCTAGAAAGT GTGACTACAC AGGTGGCTCA TAAGAAATCT
    ACTCTACAGA CATCTGCAAA GCAAATTGAG GACAGGATTT TTGAGGTGAA GCATCAACAC
    AGGAAGGTGG AAAACCAGAT CAAGATGGCC AAGATGGTTC TGATGAATGA GCTGAACAAA
    CAGGCCAACG GGCTCATAGA GGAGTTGGAG GGTATTACTA ATGAGAGAAA GCGGAAGCTG
    GAACAGCAGT TACAGAGCAT CATGGTTCTC AACCGTCAGT TTGAGCATGT GCAGAACTTC
    ATCAACTGGG CTGTCTGCAG CAAGACCAGT GTCCCTTTCC TGTTCAGCAA AGAGCTGATT
    GTGTTTCAGA TGCAGCGATT GCTGGAGGCA AACTGTAACA CAGATCCGGG CTCCCCCTGG
    AGTATCAGAT TCACCTGGGA GCCTAACTTC TGGACCAAGC AGCTGGCTTC CCTTGGCTGT
    ATAACTACTG AAGGTGGACA GCTGTCCCGG GCAGATACTC CTGCTTATGG AGGCTTACAG
    GGACCATCAT CCTTTTATCA AAGCCACCAG TCCCCAGTGG CTCAGCAAGA GACTCTTAGC
    CACCCCTCAC ATAAGTTCCA GTCTCCAGCA CTGTGTTCTT CATCTGTATG CTGCTCCCAC
    TGCTCCCCAG CCTCGCCTTC CCTCAAAGGC CAGGTCCCCC CACCCAGCAT ACACTCAGCC
    CACAGCTTTA GGCAGCCCTC AGAGATGGCA CCCCAACAGC TAGGCTCTCT GCAGTGCTCT
    ACTCTGCTGC CCAGGGAGAA GGAGCTGCCT TGCAATCCTC AGCCACCAAA ACTGATGCAG
    CCCTGGCTGG AAACCCAGCC CCCCGTGGAG CAGGAGAACA CATCCCAGCG GCTGGGGCAA
    CAGCCAACTT CCCAGCCAGT ATGCATTGTG CCCCCGCAGG ATGTTCAGCA AGGAGCCCAT
    GCCCAACCCA CCTTACAGAC ACCCTCTATC CAAGTCCAGT TTGGCCACCA CCAGAAGCTG
    AAGCTCAGCC ACTTTCAGCA GCAGCCACAG CAGCAACTGC CACCTCCGCC ACCTCTGCCA
    CCCCCACCCC AGCAGCAGCC ACTGCCACCT CTACCTCCAC CCCAGCATCC AGCTTCTAGT
    CAGCATGGGA GCCCTCCTGG GCCTGCCTGT TCCCAGAACA TGGATATAAT GCACCACAAA
    TTTGAGCTGG AGGAGATGCA GAAGGACCTG GAGCTTCTTC TTCAGGCTCA ACAGCCCAGC
    CTACAGCTGA ATCAGACCAA ATCTCCTCAG CATCTTCAGC AAACCATTGT GGGACAGATC
    AACTACATTG TGAGGCAGCC AGCACCCGTC CAGTCCCAGA GCCAAGAGGA TGCTGCACAG
    GCTTCGGATG AGCACCCCAC ATCTGAAGGT CCAAAGCCGC CTCTCCCACT TGACAAGAAT
    ACTGCTGCTA ACTTGCCCCA GGCGTCTGGG GAGGAAACCC CTCCTGGTGT CCCCCCACCA
    GACGGCACCA TCCCGCACTC CTCTCCAAGT GTGGTGAGAA AGCACTCCAC TTCAGTGAGC
    ATCATGGGCT TCTCCAACAC GCTAGAGATG GAGCTGTCAT CTACCAGGCT AGCCAGGACC
    CTGGAGCCGC AGATCCAGAG CGTGAGCAGC CTGACTGCTG CCCCTTCCCA CGTGGTCCCA
    AGCCTGCTGA GCGGCACCCC CACAACTGTG TCCAGCCTGC TGAGCGGTCA GAGCCAGGCT
    GTGCCCAGCC TGACAGTTAG TCCTCTGCAG ACCATACCCA GTCTTGTACG TGGCACACCC
    CGGACCGTGC CCGGTTTGAT GAGTGATTCC TCCCAGGCCA TCACAAGCCT AGCAAGTGAT
    CACTCACAGG CCAGGCCCAA GCTGATGTCT GGTCCCACCC AGACTCTGCC AAGTCTGGCT
    ACTTGTCCAC TGCAGAGCAT GCCTCCGGCT TCTGACACGC AACCGGAAAC TGCATCCAGT
    GGCAGTCCTG GCTCTGGCGG AGCTGTGGGG AGCCTGTGCC CTGGAGATGG GGCTGACCCC
    TCTCTTGGGA ATGCCCTGTG TAAGATGGAA AGTGAGGATT CTTCCCGCTT CACTGACTCT
    CTGGAACAGG ACCCCACAAC TCCTGGTCTG GAGGTTTCCA AGGATTTGGC TATCCCTTCA
    GAACTGGAGG AGCCAATTAA CCTCTCTGTG AAGAAACCTC CAGTGGTCCC AGCAGTCAGC
    ACAGCTATGG CTCTGCAGCA GTACCAGAAC CCAAAAGAGT ATGAGAATTT TGAAGAAGCC
    CTGGAGCTGA ATGTAAAAGA GAACCAGAGT ATCAGGCGAG AGCCTAAGAT TCCCTATGTG
    CGGCTGGAGC GGCTTAAGAT CTGTGCTGCC TCCTCGGGAG AGATGCCTGT GTTCAAGCTG
    AAGCCCCAGA AGAATGAGCA GGATGGGAGC TTCCTGCTGA TCATCGAGTG TGGCACAGAG
    TCCTCCAGCA TGTCCATTAA GGTCAGCCAG AACAACCTGC CTGATGCCAT CCAGGCCCCA
    AGTCTGGGGG GAAGAAAGGT CACTGTCACT TCTCTGGCTG GACAACAGCC ACCAGAAGGG
    GAGGGTGCAT CTCCTGAAGA ACAAAGGCTC ATCCCTCGGA CCCTTGGAGC CAAGAAGGGG
    CCCCCAGTAC CAATAGAGAA TGAGGACTTC TGTGCTGTGT GCCTCAATGG GGGCGAGCTA
    CTGTGCTGTG ACCGCTGCCC TAAGGTGTTC CACCTCTCCT GCCACCTGCC GGCCTTGCTT
    GGCTTCCCAG GGGGAGATTG GGTGTGTACC TTGTGCCGCA GCCTGACCCA GCCCGAGATG
    GAGTATGACT GTGAGAATGC CCGCTATTAC CAACCTGGGA TGCGGGACAC CCCTGGCCTG
    AGCATCTATG ACCAAAAGAA GTGTGAAAAG TTGGTTCTGT CCTTGTGCTG CAATAGCCTC
    AGCCTGCCCT TCCACGAGCC TGTCAGCCCG CTGGCCCGGC ATTACTACCA GATTATCAAG
    AGGCCCATGG ACCTGTCAAT CATCCGGAGG AAGCTGCAAA AGAAGGACCC AGCTCACTAC
    ACCACCCCAG AGGAGGTGGT GTCAGACGTC CGCCTCATGT TCTGGAACTG TGCTAAATTC
    AATTATCCTG ACTCAGAAGT TGCAGAGGCT GGCCGCTGCC TGGAAGTTTT CTTTGAGGGC
    TGGTTGAAGG AGATCTATCC AGAAAAACAG TTTGCCCAGC CGAGGCAGGA GGACTCAGAC
    TCCGAGGAGG TGTCTAGCGA GAGTGGCTAT TCCACTCCCC AGGGCTTCCC ATGGCCTCCC
    TATGTGCAGG AGGGCATCCA ACCCAAGAGG CGGCGGCGAC ATATGGAGAA TGAAAGAGCA
    AAAAGAATGT CATTCCGCTT GGCCAGCAGC GTCTCCCAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013978254.1
    CDS137..4261
    Translation

    Target ORF information:

    RefSeq Version XM_014122779.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014122779.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGGAG TGGAGCTTGC TCGATCTACA CGCTGCTGCA CACTTCAGGA TATCATGGGA 
    AGAGCCCCAG TCCCGGTCAC AAGCACAGCC ACGGATACGG GCTTGAGATT TATGGGGCTG
    CCACTAGTTG ACCAGAAACA CAGGAATGGT CAGATGGAGG CCATGATATT GACCTGCTCA
    TTGTGCCAAC AAGACCTGCA AAATGTGGGC GCCCGGTTCC TACCCTGCCA GCATTTGCTC
    TGCAAGAACT GCTTCCAGGG CTTCATCCAG GAGCTGAGAC AGGTTTCCAA AGCTCACAGG
    ACCGTTCCAC CTGAGCTCAT CTCCTGTCCT GGGTGTAAGC GAGTATATCT TACCAGGGAT
    GTAAATGAAC ATTTTTTTCT GCAGTGCTTT TCTACAGGGC GATCCAAGAT GGCCAGGAAC
    TGCTCCGAGT GCAAGGAGAA GAGGGCAGCG CATATCCTGT GCACCTACTG CAACCGCTGG
    CTGTGCAGCT CATGCACAGA GGAGCACCGG CATGGCCCCG CCCCTGGGGG CCCACTCTTT
    CCGCGGGCCC ACAAGGGACC ACCAGGAGTG AGCAGTGGTT CTGGGGACTT CGCCTTGTAC
    TGTCCTCTAC ACACACAGGA AGTGCTCAAG CTATTCTGCG AGACCTGTGA TGTGCTCACG
    TGCCATAGCT GCCTCATGGT GGAACACAAA GAACACAGGT GTAGACATCT TGAAGAAGTT
    TTGCAAAACC AGAGGATGCT TCTAGAAAGT GTGACTACAC AGGTGGCTCA TAAGAAATCT
    ACTCTACAGA CATCTGCAAA GCAAATTGAG GACAGGATTT TTGAGGTGAA GCATCAACAC
    AGGAAGGTGG AAAACCAGAT CAAGATGGCC AAGATGGTTC TGATGAATGA GCTGAACAAA
    CAGGCCAACG GGCTCATAGA GGAGTTGGAG GGTATTACTA ATGAGAGAAA GCGGAAGCTG
    GAACAGCAGT TACAGAGCAT CATGGTTCTC AACCGTCAGT TTGAGCATGT GCAGAACTTC
    ATCAACTGGG CTGTCTGCAG CAAGACCAGT GTCCCTTTCC TGTTCAGCAA AGAGCTGATT
    GTGTTTCAGA TGCAGCGATT GCTGGAGGCA AACTGTAACA CAGATCCGGG CTCCCCCTGG
    AGTATCAGAT TCACCTGGGA GCCTAACTTC TGGACCAAGC AGCTGGCTTC CCTTGGCTGT
    ATAACTACTG AAGGTGGACA GCTGTCCCGG GCAGATACTC CTGCTTATGG AGGCTTACAG
    GGACCATCAT CCTTTTATCA AAGCCACCAG TCCCCAGTGG CTCAGCAAGA GACTCTTAGC
    CACCCCTCAC ATAAGTTCCA GTCTCCAGCA CTGTGTTCTT CATCTGTATG CTGCTCCCAC
    TGCTCCCCAG CCTCGCCTTC CCTCAAAGGC CAGGTCCCCC CACCCAGCAT ACACTCAGCC
    CACAGCTTTA GGCAGCCCTC AGAGATGGCA CCCCAACAGC TAGGCTCTCT GCAGTGCTCT
    ACTCTGCTGC CCAGGGAGAA GGAGCTGCCT TGCAATCCTC AGCCACCAAA ACTGATGCAG
    CCCTGGCTGG AAACCCAGCC CCCCGTGGAG CAGGAGAACA CATCCCAGCG GCTGGGGCAA
    CAGCCAACTT CCCAGCCAGT ATGCATTGTG CCCCCGCAGG ATGTTCAGCA AGGAGCCCAT
    GCCCAACCCA CCTTACAGAC ACCCTCTATC CAAGTCCAGT TTGGCCACCA CCAGAAGCTG
    AAGCTCAGCC ACTTTCAGCA GCAGCCACAG CAGCAACTGC CACCTCCGCC ACCTCTGCCA
    CCCCCACCCC AGCAGCAGCC ACTGCCACCT CTACCTCCAC CCCAGCATCC AGCTTCTAGT
    CAGCATGGGA GCCCTCCTGG GCCTGCCTGT TCCCAGAACA TGGATATAAT GCACCACAAA
    TTTGAGCTGG AGGAGATGCA GAAGGACCTG GAGCTTCTTC TTCAGGCTCA ACAGCCCAGC
    CTACAGCTGA ATCAGACCAA ATCTCCTCAG CATCTTCAGC AAACCATTGT GGGACAGATC
    AACTACATTG TGAGGCAGCC AGCACCCGTC CAGTCCCAGA GCCAAGAGGA TGCTGCACAG
    GCTTCGGATG AGCACCCCAC ATCTGAAGGT CCAAAGCCGC CTCTCCCACT TGACAAGAAT
    ACTGCTGCTA ACTTGCCCCA GGCGTCTGGG GAGGAAACCC CTCCTGGTGT CCCCCCACCA
    GACGGCACCA TCCCGCACTC CTCTCCAAGT GTGGTGAGAA AGCACTCCAC TTCAGTGAGC
    ATCATGGGCT TCTCCAACAC GCTAGAGATG GAGCTGTCAT CTACCAGGCT AGCCAGGACC
    CTGGAGCCGC AGATCCAGAG CGTGAGCAGC CTGACTGCTG CCCCTTCCCA CGTGGTCCCA
    AGCCTGCTGA GCGGCACCCC CACAACTGTG TCCAGCCTGC TGAGCGGTCA GAGCCAGGCT
    GTGCCCAGCC TGACAGTTAG TCCTCTGCAG ACCATACCCA GTCTTGTACG TGGCACACCC
    CGGACCGTGC CCGGTTTGAT GAGTGATTCC TCCCAGGCCA TCACAAGCCT AGCAAGTGAT
    CACTCACAGG CCAGGCCCAA GCTGATGTCT GGTCCCACCC AGACTCTGCC AAGTCTGGCT
    ACTTGTCCAC TGCAGAGCAT GCCTCCGGCT TCTGACACGC AACCGGAAAC TGCATCCAGT
    GGCAGTCCTG GCTCTGGCGG AGCTGTGGGG AGCCTGTGCC CTGGAGATGG GGCTGACCCC
    TCTCTTGGGA ATGCCCTGTG TAAGATGGAA AGTGAGGATT CTTCCCGCTT CACTGACTCT
    CTGGAACAGG ACCCCACAAC TCCTGGTCTG GAGGTTTCCA AGGATTTGGC TATCCCTTCA
    GAACTGGAGG AGCCAATTAA CCTCTCTGTG AAGAAACCTC CAGTGGTCCC AGCAGTCAGC
    ACAGCTATGG CTCTGCAGCA GTACCAGAAC CCAAAAGAGT ATGAGAATTT TGAAGAAGCC
    CTGGAGCTGA ATGTAAAAGA GAACCAGAGT ATCAGGCGAG AGCCTAAGAT TCCCTATGTG
    CGGCTGGAGC GGCTTAAGAT CTGTGCTGCC TCCTCGGGAG AGATGCCTGT GTTCAAGCTG
    AAGCCCCAGA AGAATGAGCA GGATGGGAGC TTCCTGCTGA TCATCGAGTG TGGCACAGAG
    TCCTCCAGCA TGTCCATTAA GGTCAGCCAG AACAACCTGC CTGATGCCAT CCAGGCCCCA
    AGTCTGGGGG GAAGAAAGGT CACTGTCACT TCTCTGGCTG GACAACAGCC ACCAGAAGGG
    GAGGGTGCAT CTCCTGAAGA ACAAAGGCTC ATCCCTCGGA CCCTTGGAGC CAAGAAGGGG
    CCCCCAGTAC CAATAGAGAA TGAGGACTTC TGTGCTGTGT GCCTCAATGG GGGCGAGCTA
    CTGTGCTGTG ACCGCTGCCC TAAGGTGTTC CACCTCTCCT GCCACCTGCC GGCCTTGCTT
    GGCTTCCCAG GGGGAGATTG GGTGTGTACC TTGTGCCGCA GCCTGACCCA GCCCGAGATG
    GAGTATGACT GTGAGAATGC CCGCTATTAC CAACCTGGGA TGCGGGACAC CCCTGGCCTG
    AGCATCTATG ACCAAAAGAA GTGTGAAAAG TTGGTTCTGT CCTTGTGCTG CAATAGCCTC
    AGCCTGCCCT TCCACGAGCC TGTCAGCCCG CTGGCCCGGC ATTACTACCA GATTATCAAG
    AGGCCCATGG ACCTGTCAAT CATCCGGAGG AAGCTGCAAA AGAAGGACCC AGCTCACTAC
    ACCACCCCAG AGGAGGTGGT GTCAGACGTC CGCCTCATGT TCTGGAACTG TGCTAAATTC
    AATTATCCTG ACTCAGAAGT TGCAGAGGCT GGCCGCTGCC TGGAAGTTTT CTTTGAGGGC
    TGGTTGAAGG AGATCTATCC AGAAAAACAG TTTGCCCAGC CGAGGCAGGA GGACTCAGAC
    TCCGAGGAGG TGTCTAGCGA GAGTGGCTAT TCCACTCCCC AGGGCTTCCC ATGGCCTCCC
    TATGTGCAGG AGGGCATCCA ACCCAAGAGG CGGCGGCGAC ATATGGAGAA TGAAAGAGCA
    AAAAGAATGT CATTCCGCTT GGCCAGCAGC GTCTCCCAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42226
    Clone ID Related Accession (Same CDS sequence) XM_014122779.1 , XM_014122779.2
    Accession Version XM_014122779.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tripartite motif-containing protein 66 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGGAG TGGAGCTTGC TCGATCTACA CGCTGCTGCA CACTTCAGGA TATCATGGGA 
    AGAGCCCCAG TCCCGGTCAC AAGCACAGCC ACGGATACGG GCTTGAGATT TATGGGGCTG
    CCACTAGTTG ACCAGAAACA CAGGAATGGT CAGATGGAGG CCATGATATT GACCTGCTCA
    TTGTGCCAAC AAGACCTGCA AAATGTGGGC GCCCGGTTCC TACCCTGCCA GCATTTGCTC
    TGCAAGAACT GCTTCCAGGG CTTCATCCAG GAGCTGAGAC AGGTTTCCAA AGCTCACAGG
    ACCGTTCCAC CTGAGCTCAT CTCCTGTCCT GGGTGTAAGC GAGTATATCT TACCAGGGAT
    GTAAATGAAC ATTTTTTTCT GCAGTGCTTT TCTACAGGGC GATCCAAGAT GGCCAGGAAC
    TGCTCCGAGT GCAAGGAGAA GAGGGCAGCG CATATCCTGT GCACCTACTG CAACCGCTGG
    CTGTGCAGCT CATGCACAGA GGAGCACCGG CATGGCCCCG CCCCTGGGGG CCCACTCTTT
    CCGCGGGCCC ACAAGGGACC ACCAGGAGTG AGCAGTGGTT CTGGGGACTT CGCCTTGTAC
    TGTCCTCTAC ACACACAGGA AGTGCTCAAG CTATTCTGCG AGACCTGTGA TGTGCTCACG
    TGCCATAGCT GCCTCATGGT GGAACACAAA GAACACAGGT GTAGACATCT TGAAGAAGTT
    TTGCAAAACC AGAGGATGCT TCTAGAAAGT GTGACTACAC AGGTGGCTCA TAAGAAATCT
    ACTCTACAGA CATCTGCAAA GCAAATTGAG GACAGGATTT TTGAGGTGAA GCATCAACAC
    AGGAAGGTGG AAAACCAGAT CAAGATGGCC AAGATGGTTC TGATGAATGA GCTGAACAAA
    CAGGCCAACG GGCTCATAGA GGAGTTGGAG GGTATTACTA ATGAGAGAAA GCGGAAGCTG
    GAACAGCAGT TACAGAGCAT CATGGTTCTC AACCGTCAGT TTGAGCATGT GCAGAACTTC
    ATCAACTGGG CTGTCTGCAG CAAGACCAGT GTCCCTTTCC TGTTCAGCAA AGAGCTGATT
    GTGTTTCAGA TGCAGCGATT GCTGGAGGCA AACTGTAACA CAGATCCGGG CTCCCCCTGG
    AGTATCAGAT TCACCTGGGA GCCTAACTTC TGGACCAAGC AGCTGGCTTC CCTTGGCTGT
    ATAACTACTG AAGGTGGACA GCTGTCCCGG GCAGATACTC CTGCTTATGG AGGCTTACAG
    GGACCATCAT CCTTTTATCA AAGCCACCAG TCCCCAGTGG CTCAGCAAGA GACTCTTAGC
    CACCCCTCAC ATAAGTTCCA GTCTCCAGCA CTGTGTTCTT CATCTGTATG CTGCTCCCAC
    TGCTCCCCAG CCTCGCCTTC CCTCAAAGGC CAGGTCCCCC CACCCAGCAT ACACTCAGCC
    CACAGCTTTA GGCAGCCCTC AGAGATGGCA CCCCAACAGC TAGGCTCTCT GCAGTGCTCT
    ACTCTGCTGC CCAGGGAGAA GGAGCTGCCT TGCAATCCTC AGCCACCAAA ACTGATGCAG
    CCCTGGCTGG AAACCCAGCC CCCCGTGGAG CAGGAGAACA CATCCCAGCG GCTGGGGCAA
    CAGCCAACTT CCCAGCCAGT ATGCATTGTG CCCCCGCAGG ATGTTCAGCA AGGAGCCCAT
    GCCCAACCCA CCTTACAGAC ACCCTCTATC CAAGTCCAGT TTGGCCACCA CCAGAAGCTG
    AAGCTCAGCC ACTTTCAGCA GCAGCCACAG CAGCAACTGC CACCTCCGCC ACCTCTGCCA
    CCCCCACCCC AGCAGCAGCC ACTGCCACCT CTACCTCCAC CCCAGCATCC AGCTTCTAGT
    CAGCATGGGA GCCCTCCTGG GCCTGCCTGT TCCCAGAACA TGGATATAAT GCACCACAAA
    TTTGAGCTGG AGGAGATGCA GAAGGACCTG GAGCTTCTTC TTCAGGCTCA ACAGCCCAGC
    CTACAGCTGA ATCAGACCAA ATCTCCTCAG CATCTTCAGC AAACCATTGT GGGACAGATC
    AACTACATTG TGAGGCAGCC AGCACCCGTC CAGTCCCAGA GCCAAGAGGA TGCTGCACAG
    GCTTCGGATG AGCACCCCAC ATCTGAAGGT CCAAAGCCGC CTCTCCCACT TGACAAGAAT
    ACTGCTGCTA ACTTGCCCCA GGCGTCTGGG GAGGAAACCC CTCCTGGTGT CCCCCCACCA
    GACGGCACCA TCCCGCACTC CTCTCCAAGT GTGGTGAGAA AGCACTCCAC TTCAGTGAGC
    ATCATGGGCT TCTCCAACAC GCTAGAGATG GAGCTGTCAT CTACCAGGCT AGCCAGGACC
    CTGGAGCCGC AGATCCAGAG CGTGAGCAGC CTGACTGCTG CCCCTTCCCA CGTGGTCCCA
    AGCCTGCTGA GCGGCACCCC CACAACTGTG TCCAGCCTGC TGAGCGGTCA GAGCCAGGCT
    GTGCCCAGCC TGACAGTTAG TCCTCTGCAG ACCATACCCA GTCTTGTACG TGGCACACCC
    CGGACCGTGC CCGGTTTGAT GAGTGATTCC TCCCAGGCCA TCACAAGCCT AGCAAGTGAT
    CACTCACAGG CCAGGCCCAA GCTGATGTCT GGTCCCACCC AGACTCTGCC AAGTCTGGCT
    ACTTGTCCAC TGCAGAGCAT GCCTCCGGCT TCTGACACGC AACCGGAAAC TGCATCCAGT
    GGCAGTCCTG GCTCTGGCGG AGCTGTGGGG AGCCTGTGCC CTGGAGATGG GGCTGACCCC
    TCTCTTGGGA ATGCCCTGTG TAAGATGGAA AGTGAGGATT CTTCCCGCTT CACTGACTCT
    CTGGAACAGG ACCCCACAAC TCCTGGTCTG GAGGTTTCCA AGGATTTGGC TATCCCTTCA
    GAACTGGAGG AGCCAATTAA CCTCTCTGTG AAGAAACCTC CAGTGGTCCC AGCAGTCAGC
    ACAGCTATGG CTCTGCAGCA GTACCAGAAC CCAAAAGAGT ATGAGAATTT TGAAGAAGCC
    CTGGAGCTGA ATGTAAAAGA GAACCAGAGT ATCAGGCGAG AGCCTAAGAT TCCCTATGTG
    CGGCTGGAGC GGCTTAAGAT CTGTGCTGCC TCCTCGGGAG AGATGCCTGT GTTCAAGCTG
    AAGCCCCAGA AGAATGAGCA GGATGGGAGC TTCCTGCTGA TCATCGAGTG TGGCACAGAG
    TCCTCCAGCA TGTCCATTAA GGTCAGCCAG AACAACCTGC CTGATGCCAT CCAGGCCCCA
    AGTCTGGGGG GAAGAAAGGT CACTGTCACT TCTCTGGCTG GACAACAGCC ACCAGAAGGG
    GAGGGTGCAT CTCCTGAAGA ACAAAGGCTC ATCCCTCGGA CCCTTGGAGC CAAGAAGGGG
    CCCCCAGTAC CAATAGAGAA TGAGGACTTC TGTGCTGTGT GCCTCAATGG GGGCGAGCTA
    CTGTGCTGTG ACCGCTGCCC TAAGGTGTTC CACCTCTCCT GCCACCTGCC GGCCTTGCTT
    GGCTTCCCAG GGGGAGATTG GGTGTGTACC TTGTGCCGCA GCCTGACCCA GCCCGAGATG
    GAGTATGACT GTGAGAATGC CCGCTATTAC CAACCTGGGA TGCGGGACAC CCCTGGCCTG
    AGCATCTATG ACCAAAAGAA GTGTGAAAAG TTGGTTCTGT CCTTGTGCTG CAATAGCCTC
    AGCCTGCCCT TCCACGAGCC TGTCAGCCCG CTGGCCCGGC ATTACTACCA GATTATCAAG
    AGGCCCATGG ACCTGTCAAT CATCCGGAGG AAGCTGCAAA AGAAGGACCC AGCTCACTAC
    ACCACCCCAG AGGAGGTGGT GTCAGACGTC CGCCTCATGT TCTGGAACTG TGCTAAATTC
    AATTATCCTG ACTCAGAAGT TGCAGAGGCT GGCCGCTGCC TGGAAGTTTT CTTTGAGGGC
    TGGTTGAAGG AGATCTATCC AGAAAAACAG TTTGCCCAGC CGAGGCAGGA GGACTCAGAC
    TCCGAGGAGG TGTCTAGCGA GAGTGGCTAT TCCACTCCCC AGGGCTTCCC ATGGCCTCCC
    TATGTGCAGG AGGGCATCCA ACCCAAGAGG CGGCGGCGAC ATATGGAGAA TGAAAGAGCA
    AAAAGAATGT CATTCCGCTT GGCCAGCAGC GTCTCCCAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013978254.1
    CDS185..4309
    Translation

    Target ORF information:

    RefSeq Version XM_014122779.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014122779.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGGAG TGGAGCTTGC TCGATCTACA CGCTGCTGCA CACTTCAGGA TATCATGGGA 
    AGAGCCCCAG TCCCGGTCAC AAGCACAGCC ACGGATACGG GCTTGAGATT TATGGGGCTG
    CCACTAGTTG ACCAGAAACA CAGGAATGGT CAGATGGAGG CCATGATATT GACCTGCTCA
    TTGTGCCAAC AAGACCTGCA AAATGTGGGC GCCCGGTTCC TACCCTGCCA GCATTTGCTC
    TGCAAGAACT GCTTCCAGGG CTTCATCCAG GAGCTGAGAC AGGTTTCCAA AGCTCACAGG
    ACCGTTCCAC CTGAGCTCAT CTCCTGTCCT GGGTGTAAGC GAGTATATCT TACCAGGGAT
    GTAAATGAAC ATTTTTTTCT GCAGTGCTTT TCTACAGGGC GATCCAAGAT GGCCAGGAAC
    TGCTCCGAGT GCAAGGAGAA GAGGGCAGCG CATATCCTGT GCACCTACTG CAACCGCTGG
    CTGTGCAGCT CATGCACAGA GGAGCACCGG CATGGCCCCG CCCCTGGGGG CCCACTCTTT
    CCGCGGGCCC ACAAGGGACC ACCAGGAGTG AGCAGTGGTT CTGGGGACTT CGCCTTGTAC
    TGTCCTCTAC ACACACAGGA AGTGCTCAAG CTATTCTGCG AGACCTGTGA TGTGCTCACG
    TGCCATAGCT GCCTCATGGT GGAACACAAA GAACACAGGT GTAGACATCT TGAAGAAGTT
    TTGCAAAACC AGAGGATGCT TCTAGAAAGT GTGACTACAC AGGTGGCTCA TAAGAAATCT
    ACTCTACAGA CATCTGCAAA GCAAATTGAG GACAGGATTT TTGAGGTGAA GCATCAACAC
    AGGAAGGTGG AAAACCAGAT CAAGATGGCC AAGATGGTTC TGATGAATGA GCTGAACAAA
    CAGGCCAACG GGCTCATAGA GGAGTTGGAG GGTATTACTA ATGAGAGAAA GCGGAAGCTG
    GAACAGCAGT TACAGAGCAT CATGGTTCTC AACCGTCAGT TTGAGCATGT GCAGAACTTC
    ATCAACTGGG CTGTCTGCAG CAAGACCAGT GTCCCTTTCC TGTTCAGCAA AGAGCTGATT
    GTGTTTCAGA TGCAGCGATT GCTGGAGGCA AACTGTAACA CAGATCCGGG CTCCCCCTGG
    AGTATCAGAT TCACCTGGGA GCCTAACTTC TGGACCAAGC AGCTGGCTTC CCTTGGCTGT
    ATAACTACTG AAGGTGGACA GCTGTCCCGG GCAGATACTC CTGCTTATGG AGGCTTACAG
    GGACCATCAT CCTTTTATCA AAGCCACCAG TCCCCAGTGG CTCAGCAAGA GACTCTTAGC
    CACCCCTCAC ATAAGTTCCA GTCTCCAGCA CTGTGTTCTT CATCTGTATG CTGCTCCCAC
    TGCTCCCCAG CCTCGCCTTC CCTCAAAGGC CAGGTCCCCC CACCCAGCAT ACACTCAGCC
    CACAGCTTTA GGCAGCCCTC AGAGATGGCA CCCCAACAGC TAGGCTCTCT GCAGTGCTCT
    ACTCTGCTGC CCAGGGAGAA GGAGCTGCCT TGCAATCCTC AGCCACCAAA ACTGATGCAG
    CCCTGGCTGG AAACCCAGCC CCCCGTGGAG CAGGAGAACA CATCCCAGCG GCTGGGGCAA
    CAGCCAACTT CCCAGCCAGT ATGCATTGTG CCCCCGCAGG ATGTTCAGCA AGGAGCCCAT
    GCCCAACCCA CCTTACAGAC ACCCTCTATC CAAGTCCAGT TTGGCCACCA CCAGAAGCTG
    AAGCTCAGCC ACTTTCAGCA GCAGCCACAG CAGCAACTGC CACCTCCGCC ACCTCTGCCA
    CCCCCACCCC AGCAGCAGCC ACTGCCACCT CTACCTCCAC CCCAGCATCC AGCTTCTAGT
    CAGCATGGGA GCCCTCCTGG GCCTGCCTGT TCCCAGAACA TGGATATAAT GCACCACAAA
    TTTGAGCTGG AGGAGATGCA GAAGGACCTG GAGCTTCTTC TTCAGGCTCA ACAGCCCAGC
    CTACAGCTGA ATCAGACCAA ATCTCCTCAG CATCTTCAGC AAACCATTGT GGGACAGATC
    AACTACATTG TGAGGCAGCC AGCACCCGTC CAGTCCCAGA GCCAAGAGGA TGCTGCACAG
    GCTTCGGATG AGCACCCCAC ATCTGAAGGT CCAAAGCCGC CTCTCCCACT TGACAAGAAT
    ACTGCTGCTA ACTTGCCCCA GGCGTCTGGG GAGGAAACCC CTCCTGGTGT CCCCCCACCA
    GACGGCACCA TCCCGCACTC CTCTCCAAGT GTGGTGAGAA AGCACTCCAC TTCAGTGAGC
    ATCATGGGCT TCTCCAACAC GCTAGAGATG GAGCTGTCAT CTACCAGGCT AGCCAGGACC
    CTGGAGCCGC AGATCCAGAG CGTGAGCAGC CTGACTGCTG CCCCTTCCCA CGTGGTCCCA
    AGCCTGCTGA GCGGCACCCC CACAACTGTG TCCAGCCTGC TGAGCGGTCA GAGCCAGGCT
    GTGCCCAGCC TGACAGTTAG TCCTCTGCAG ACCATACCCA GTCTTGTACG TGGCACACCC
    CGGACCGTGC CCGGTTTGAT GAGTGATTCC TCCCAGGCCA TCACAAGCCT AGCAAGTGAT
    CACTCACAGG CCAGGCCCAA GCTGATGTCT GGTCCCACCC AGACTCTGCC AAGTCTGGCT
    ACTTGTCCAC TGCAGAGCAT GCCTCCGGCT TCTGACACGC AACCGGAAAC TGCATCCAGT
    GGCAGTCCTG GCTCTGGCGG AGCTGTGGGG AGCCTGTGCC CTGGAGATGG GGCTGACCCC
    TCTCTTGGGA ATGCCCTGTG TAAGATGGAA AGTGAGGATT CTTCCCGCTT CACTGACTCT
    CTGGAACAGG ACCCCACAAC TCCTGGTCTG GAGGTTTCCA AGGATTTGGC TATCCCTTCA
    GAACTGGAGG AGCCAATTAA CCTCTCTGTG AAGAAACCTC CAGTGGTCCC AGCAGTCAGC
    ACAGCTATGG CTCTGCAGCA GTACCAGAAC CCAAAAGAGT ATGAGAATTT TGAAGAAGCC
    CTGGAGCTGA ATGTAAAAGA GAACCAGAGT ATCAGGCGAG AGCCTAAGAT TCCCTATGTG
    CGGCTGGAGC GGCTTAAGAT CTGTGCTGCC TCCTCGGGAG AGATGCCTGT GTTCAAGCTG
    AAGCCCCAGA AGAATGAGCA GGATGGGAGC TTCCTGCTGA TCATCGAGTG TGGCACAGAG
    TCCTCCAGCA TGTCCATTAA GGTCAGCCAG AACAACCTGC CTGATGCCAT CCAGGCCCCA
    AGTCTGGGGG GAAGAAAGGT CACTGTCACT TCTCTGGCTG GACAACAGCC ACCAGAAGGG
    GAGGGTGCAT CTCCTGAAGA ACAAAGGCTC ATCCCTCGGA CCCTTGGAGC CAAGAAGGGG
    CCCCCAGTAC CAATAGAGAA TGAGGACTTC TGTGCTGTGT GCCTCAATGG GGGCGAGCTA
    CTGTGCTGTG ACCGCTGCCC TAAGGTGTTC CACCTCTCCT GCCACCTGCC GGCCTTGCTT
    GGCTTCCCAG GGGGAGATTG GGTGTGTACC TTGTGCCGCA GCCTGACCCA GCCCGAGATG
    GAGTATGACT GTGAGAATGC CCGCTATTAC CAACCTGGGA TGCGGGACAC CCCTGGCCTG
    AGCATCTATG ACCAAAAGAA GTGTGAAAAG TTGGTTCTGT CCTTGTGCTG CAATAGCCTC
    AGCCTGCCCT TCCACGAGCC TGTCAGCCCG CTGGCCCGGC ATTACTACCA GATTATCAAG
    AGGCCCATGG ACCTGTCAAT CATCCGGAGG AAGCTGCAAA AGAAGGACCC AGCTCACTAC
    ACCACCCCAG AGGAGGTGGT GTCAGACGTC CGCCTCATGT TCTGGAACTG TGCTAAATTC
    AATTATCCTG ACTCAGAAGT TGCAGAGGCT GGCCGCTGCC TGGAAGTTTT CTTTGAGGGC
    TGGTTGAAGG AGATCTATCC AGAAAAACAG TTTGCCCAGC CGAGGCAGGA GGACTCAGAC
    TCCGAGGAGG TGTCTAGCGA GAGTGGCTAT TCCACTCCCC AGGGCTTCCC ATGGCCTCCC
    TATGTGCAGG AGGGCATCCA ACCCAAGAGG CGGCGGCGAC ATATGGAGAA TGAAAGAGCA
    AAAAGAATGT CATTCCGCTT GGCCAGCAGC GTCTCCCAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42227
    Clone ID Related Accession (Same CDS sequence) XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
    Accession Version XM_014122784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tripartite motif-containing protein 66 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013978259.1
    CDS140..3856
    Translation

    Target ORF information:

    RefSeq Version XM_014122784.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014122784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42227
    Clone ID Related Accession (Same CDS sequence) XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
    Accession Version XM_014122783.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tripartite motif-containing protein 66 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013978258.1
    CDS739..4455
    Translation

    Target ORF information:

    RefSeq Version XM_014122783.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014122783.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42227
    Clone ID Related Accession (Same CDS sequence) XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
    Accession Version XM_014122782.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tripartite motif-containing protein 66 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013978257.1
    CDS690..4406
    Translation

    Target ORF information:

    RefSeq Version XM_014122782.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014122782.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42227
    Clone ID Related Accession (Same CDS sequence) XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
    Accession Version XM_014122780.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tripartite motif-containing protein 66 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013978255.1
    CDS673..4389
    Translation

    Target ORF information:

    RefSeq Version XM_014122780.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014122780.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42227
    Clone ID Related Accession (Same CDS sequence) XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
    Accession Version XM_014122781.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tripartite motif-containing protein 66 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013978256.1
    CDS321..4037
    Translation

    Target ORF information:

    RefSeq Version XM_014122781.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014122781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42227
    Clone ID Related Accession (Same CDS sequence) XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
    Accession Version XM_014122783.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tripartite motif-containing protein 66 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013978258.1
    CDS756..4472
    Translation

    Target ORF information:

    RefSeq Version XM_014122783.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014122783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42227
    Clone ID Related Accession (Same CDS sequence) XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
    Accession Version XM_014122780.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tripartite motif-containing protein 66 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013978255.1
    CDS656..4372
    Translation

    Target ORF information:

    RefSeq Version XM_014122780.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014122780.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42227
    Clone ID Related Accession (Same CDS sequence) XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
    Accession Version XM_022407812.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tripartite motif-containing protein 66 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022263520.1
    CDS118..3834
    Translation

    Target ORF information:

    RefSeq Version XM_022407812.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022407812.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42227
    Clone ID Related Accession (Same CDS sequence) XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
    Accession Version XM_014122782.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tripartite motif-containing protein 66 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013978257.1
    CDS707..4423
    Translation

    Target ORF information:

    RefSeq Version XM_014122782.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014122782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
    TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG
    GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC
    TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT
    GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT
    CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT
    CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG
    AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT
    GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA
    AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT
    GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC
    AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG
    GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT
    TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT
    GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA
    GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA
    TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC
    ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT
    CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA
    AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG
    CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG
    CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC
    CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG
    CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT
    CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA
    ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT
    CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT
    GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG
    GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA
    CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT
    GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC
    ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG
    CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC
    CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT
    CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA
    CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC
    CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG
    CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA
    ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT
    GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC
    TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG
    GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC
    CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT
    TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG
    ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT
    GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG
    TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC
    ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG
    CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA
    GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT
    GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG
    CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC
    CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC
    ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC
    TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC
    CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC
    CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC
    TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT
    TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG
    GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC
    CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG
    AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23168
    Clone ID Related Accession (Same CDS sequence) XM_005633636.2 , XM_005633636.3
    Accession Version XM_005633636.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tripartite motif-containing protein 66 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
    CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA
    AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC
    AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA
    AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC
    TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC
    TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG
    CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG
    AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG
    GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA
    CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA
    GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG
    GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT
    CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG
    CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC
    AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG
    GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC
    ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC
    AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG
    CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG
    CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG
    CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA
    AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG
    TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC
    CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA
    GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG
    GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC
    CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA
    TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA
    CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG
    CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA
    CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG
    CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG
    AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA
    TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA
    GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA
    TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG
    GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC
    TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG
    CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC
    GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC
    ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT
    CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG
    AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG
    CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG
    CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA
    GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT
    AAGATGGAAA GTGAGGATTC TTCCCGCTTC ACTGACTCTC TGGAACAGGA CCCCACAACT
    CCTGGTCTGG AGGTTTCCAA GGATTTGGCT ATCCCTTCAG AACTGGAGGA GCCAATTAAC
    CTCTCTGTGA AGAAACCTCC AGTGGTCCCA GCAGTCAGCA CAGCTATGGC TCTGCAGCAG
    TACCAGAACC CAAAAGAGTA TGAGAATTTT GAAGAAGCCC TGGAGCTGAA TGTAAAAGAG
    AACCAGAGTA TCAGGCGAGA GCCTAAGATT CCCTATGTGC GGCTGGAGCG GCTTAAGATC
    TGTGCTGCCT CCTCGGGAGA GATGCCTGTG TTCAAGCTGA AGCCCCAGAA GAATGAGCAG
    GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACAGAGT CCTCCAGCAT GTCCATTAAG
    GTCAGCCAGA ACAACCTGCC TGATGCCATC CAGGCCCCAA GTCTGGGGGG AAGAAAGGTC
    ACTGTCACTT CTCTGGCTGG ACAACAGCCA CCAGAAGGGG AGGGTGCATC TCCTGAAGAA
    CAAAGGCTCA TCCCTCGGAC CCTTGGAGCC AAGAAGGGGC CCCCAGTACC AATAGAGAAT
    GAGGACTTCT GTGCTGTGTG CCTCAATGGG GGCGAGCTAC TGTGCTGTGA CCGCTGCCCT
    AAGGTGTTCC ACCTCTCCTG CCACCTGCCG GCCTTGCTTG GCTTCCCAGG GGGAGATTGG
    GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTATGACTG TGAGAATGCC
    CGCTATTACC AACCTGGGAT GCGGGACACC CCTGGCCTGA GCATCTATGA CCAAAAGAAG
    TGTGAAAAGT TGGTTCTGTC CTTGTGCTGC AATAGCCTCA GCCTGCCCTT CCACGAGCCT
    GTCAGCCCGC TGGCCCGGCA TTACTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC
    ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTACA CCACCCCAGA GGAGGTGGTG
    TCAGACGTCC GCCTCATGTT CTGGAACTGT GCTAAATTCA ATTATCCTGA CTCAGAAGTT
    GCAGAGGCTG GCCGCTGCCT GGAAGTTTTC TTTGAGGGCT GGTTGAAGGA GATCTATCCA
    GAAAAACAGT TTGCCCAGCC GAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGCGAG
    AGTGGCTATT CCACTCCCCA GGGCTTCCCA TGGCCTCCCT ATGTGCAGGA GGGCATCCAA
    CCCAAGAGGC GGCGGCGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTCCGCTTG
    GCCAGCAGCG TCTCCCAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005633693.1
    CDS39..4202
    Translation

    Target ORF information:

    RefSeq Version XM_005633636.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633636.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
    CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA
    AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC
    AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA
    AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC
    TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC
    TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG
    CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG
    AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG
    GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA
    CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA
    GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG
    GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT
    CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG
    CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC
    AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG
    GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC
    ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC
    AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG
    CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG
    CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG
    CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA
    AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG
    TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC
    CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA
    GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG
    GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC
    CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA
    TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA
    CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG
    CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA
    CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG
    CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG
    AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA
    TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA
    GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA
    TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG
    GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC
    TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG
    CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC
    GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC
    ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT
    CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG
    AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG
    CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG
    CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA
    GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT
    AAGATGGAAA GTGAGGATTC TTCCCGCTTC ACTGACTCTC TGGAACAGGA CCCCACAACT
    CCTGGTCTGG AGGTTTCCAA GGATTTGGCT ATCCCTTCAG AACTGGAGGA GCCAATTAAC
    CTCTCTGTGA AGAAACCTCC AGTGGTCCCA GCAGTCAGCA CAGCTATGGC TCTGCAGCAG
    TACCAGAACC CAAAAGAGTA TGAGAATTTT GAAGAAGCCC TGGAGCTGAA TGTAAAAGAG
    AACCAGAGTA TCAGGCGAGA GCCTAAGATT CCCTATGTGC GGCTGGAGCG GCTTAAGATC
    TGTGCTGCCT CCTCGGGAGA GATGCCTGTG TTCAAGCTGA AGCCCCAGAA GAATGAGCAG
    GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACAGAGT CCTCCAGCAT GTCCATTAAG
    GTCAGCCAGA ACAACCTGCC TGATGCCATC CAGGCCCCAA GTCTGGGGGG AAGAAAGGTC
    ACTGTCACTT CTCTGGCTGG ACAACAGCCA CCAGAAGGGG AGGGTGCATC TCCTGAAGAA
    CAAAGGCTCA TCCCTCGGAC CCTTGGAGCC AAGAAGGGGC CCCCAGTACC AATAGAGAAT
    GAGGACTTCT GTGCTGTGTG CCTCAATGGG GGCGAGCTAC TGTGCTGTGA CCGCTGCCCT
    AAGGTGTTCC ACCTCTCCTG CCACCTGCCG GCCTTGCTTG GCTTCCCAGG GGGAGATTGG
    GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTATGACTG TGAGAATGCC
    CGCTATTACC AACCTGGGAT GCGGGACACC CCTGGCCTGA GCATCTATGA CCAAAAGAAG
    TGTGAAAAGT TGGTTCTGTC CTTGTGCTGC AATAGCCTCA GCCTGCCCTT CCACGAGCCT
    GTCAGCCCGC TGGCCCGGCA TTACTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC
    ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTACA CCACCCCAGA GGAGGTGGTG
    TCAGACGTCC GCCTCATGTT CTGGAACTGT GCTAAATTCA ATTATCCTGA CTCAGAAGTT
    GCAGAGGCTG GCCGCTGCCT GGAAGTTTTC TTTGAGGGCT GGTTGAAGGA GATCTATCCA
    GAAAAACAGT TTGCCCAGCC GAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGCGAG
    AGTGGCTATT CCACTCCCCA GGGCTTCCCA TGGCCTCCCT ATGTGCAGGA GGGCATCCAA
    CCCAAGAGGC GGCGGCGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTCCGCTTG
    GCCAGCAGCG TCTCCCAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23168
    Clone ID Related Accession (Same CDS sequence) XM_005633636.2 , XM_005633636.3
    Accession Version XM_005633636.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tripartite motif-containing protein 66 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633636.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
    CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA
    AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC
    AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA
    AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC
    TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC
    TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG
    CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG
    AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG
    GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA
    CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA
    GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG
    GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT
    CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG
    CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC
    AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG
    GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC
    ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC
    AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG
    CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG
    CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG
    CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA
    AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG
    TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC
    CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA
    GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG
    GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC
    CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA
    TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA
    CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG
    CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA
    CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG
    CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG
    AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA
    TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA
    GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA
    TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG
    GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC
    TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG
    CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC
    GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC
    ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT
    CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG
    AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG
    CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG
    CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA
    GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT
    AAGATGGAAA GTGAGGATTC TTCCCGCTTC ACTGACTCTC TGGAACAGGA CCCCACAACT
    CCTGGTCTGG AGGTTTCCAA GGATTTGGCT ATCCCTTCAG AACTGGAGGA GCCAATTAAC
    CTCTCTGTGA AGAAACCTCC AGTGGTCCCA GCAGTCAGCA CAGCTATGGC TCTGCAGCAG
    TACCAGAACC CAAAAGAGTA TGAGAATTTT GAAGAAGCCC TGGAGCTGAA TGTAAAAGAG
    AACCAGAGTA TCAGGCGAGA GCCTAAGATT CCCTATGTGC GGCTGGAGCG GCTTAAGATC
    TGTGCTGCCT CCTCGGGAGA GATGCCTGTG TTCAAGCTGA AGCCCCAGAA GAATGAGCAG
    GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACAGAGT CCTCCAGCAT GTCCATTAAG
    GTCAGCCAGA ACAACCTGCC TGATGCCATC CAGGCCCCAA GTCTGGGGGG AAGAAAGGTC
    ACTGTCACTT CTCTGGCTGG ACAACAGCCA CCAGAAGGGG AGGGTGCATC TCCTGAAGAA
    CAAAGGCTCA TCCCTCGGAC CCTTGGAGCC AAGAAGGGGC CCCCAGTACC AATAGAGAAT
    GAGGACTTCT GTGCTGTGTG CCTCAATGGG GGCGAGCTAC TGTGCTGTGA CCGCTGCCCT
    AAGGTGTTCC ACCTCTCCTG CCACCTGCCG GCCTTGCTTG GCTTCCCAGG GGGAGATTGG
    GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTATGACTG TGAGAATGCC
    CGCTATTACC AACCTGGGAT GCGGGACACC CCTGGCCTGA GCATCTATGA CCAAAAGAAG
    TGTGAAAAGT TGGTTCTGTC CTTGTGCTGC AATAGCCTCA GCCTGCCCTT CCACGAGCCT
    GTCAGCCCGC TGGCCCGGCA TTACTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC
    ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTACA CCACCCCAGA GGAGGTGGTG
    TCAGACGTCC GCCTCATGTT CTGGAACTGT GCTAAATTCA ATTATCCTGA CTCAGAAGTT
    GCAGAGGCTG GCCGCTGCCT GGAAGTTTTC TTTGAGGGCT GGTTGAAGGA GATCTATCCA
    GAAAAACAGT TTGCCCAGCC GAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGCGAG
    AGTGGCTATT CCACTCCCCA GGGCTTCCCA TGGCCTCCCT ATGTGCAGGA GGGCATCCAA
    CCCAAGAGGC GGCGGCGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTCCGCTTG
    GCCAGCAGCG TCTCCCAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005633693.1
    CDS94..4257
    Translation

    Target ORF information:

    RefSeq Version XM_005633636.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633636.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
    CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA
    AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC
    AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA
    AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC
    TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC
    TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG
    CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG
    AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG
    GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA
    CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA
    GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG
    GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT
    CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG
    CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC
    AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG
    GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC
    ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC
    AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG
    CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG
    CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG
    CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA
    AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG
    TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC
    CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA
    GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG
    GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC
    CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA
    TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA
    CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG
    CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA
    CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG
    CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG
    AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA
    TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA
    GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA
    TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG
    GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC
    TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG
    CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC
    GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC
    ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT
    CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG
    AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG
    CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG
    CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA
    GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT
    AAGATGGAAA GTGAGGATTC TTCCCGCTTC ACTGACTCTC TGGAACAGGA CCCCACAACT
    CCTGGTCTGG AGGTTTCCAA GGATTTGGCT ATCCCTTCAG AACTGGAGGA GCCAATTAAC
    CTCTCTGTGA AGAAACCTCC AGTGGTCCCA GCAGTCAGCA CAGCTATGGC TCTGCAGCAG
    TACCAGAACC CAAAAGAGTA TGAGAATTTT GAAGAAGCCC TGGAGCTGAA TGTAAAAGAG
    AACCAGAGTA TCAGGCGAGA GCCTAAGATT CCCTATGTGC GGCTGGAGCG GCTTAAGATC
    TGTGCTGCCT CCTCGGGAGA GATGCCTGTG TTCAAGCTGA AGCCCCAGAA GAATGAGCAG
    GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACAGAGT CCTCCAGCAT GTCCATTAAG
    GTCAGCCAGA ACAACCTGCC TGATGCCATC CAGGCCCCAA GTCTGGGGGG AAGAAAGGTC
    ACTGTCACTT CTCTGGCTGG ACAACAGCCA CCAGAAGGGG AGGGTGCATC TCCTGAAGAA
    CAAAGGCTCA TCCCTCGGAC CCTTGGAGCC AAGAAGGGGC CCCCAGTACC AATAGAGAAT
    GAGGACTTCT GTGCTGTGTG CCTCAATGGG GGCGAGCTAC TGTGCTGTGA CCGCTGCCCT
    AAGGTGTTCC ACCTCTCCTG CCACCTGCCG GCCTTGCTTG GCTTCCCAGG GGGAGATTGG
    GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTATGACTG TGAGAATGCC
    CGCTATTACC AACCTGGGAT GCGGGACACC CCTGGCCTGA GCATCTATGA CCAAAAGAAG
    TGTGAAAAGT TGGTTCTGTC CTTGTGCTGC AATAGCCTCA GCCTGCCCTT CCACGAGCCT
    GTCAGCCCGC TGGCCCGGCA TTACTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC
    ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTACA CCACCCCAGA GGAGGTGGTG
    TCAGACGTCC GCCTCATGTT CTGGAACTGT GCTAAATTCA ATTATCCTGA CTCAGAAGTT
    GCAGAGGCTG GCCGCTGCCT GGAAGTTTTC TTTGAGGGCT GGTTGAAGGA GATCTATCCA
    GAAAAACAGT TTGCCCAGCC GAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGCGAG
    AGTGGCTATT CCACTCCCCA GGGCTTCCCA TGGCCTCCCT ATGTGCAGGA GGGCATCCAA
    CCCAAGAGGC GGCGGCGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTCCGCTTG
    GCCAGCAGCG TCTCCCAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23169
    Clone ID Related Accession (Same CDS sequence) XM_005633637.2 , XM_005633637.3
    Accession Version XM_005633637.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tripartite motif-containing protein 66 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
    CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA
    AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC
    AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA
    AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC
    TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC
    TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG
    CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG
    AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG
    GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA
    CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA
    GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG
    GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT
    CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG
    CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC
    AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG
    GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC
    ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC
    AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG
    CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG
    CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG
    CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA
    AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG
    TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC
    CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA
    GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG
    GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC
    CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA
    TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA
    CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG
    CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA
    CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG
    CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG
    AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA
    TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA
    GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA
    TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG
    GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC
    TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG
    CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC
    GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC
    ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT
    CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG
    AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG
    CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG
    CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA
    GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT
    AAGGACCCCA CAACTCCTGG TCTGGAGGTT TCCAAGGATT TGGCTATCCC TTCAGAACTG
    GAGGAGCCAA TTAACCTCTC TGTGAAGAAA CCTCCAGTGG TCCCAGCAGT CAGCACAGCT
    ATGGCTCTGC AGCAGTACCA GAACCCAAAA GAGTATGAGA ATTTTGAAGA AGCCCTGGAG
    CTGAATGTAA AAGAGAACCA GAGTATCAGG CGAGAGCCTA AGATTCCCTA TGTGCGGCTG
    GAGCGGCTTA AGATCTGTGC TGCCTCCTCG GGAGAGATGC CTGTGTTCAA GCTGAAGCCC
    CAGAAGAATG AGCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC AGAGTCCTCC
    AGCATGTCCA TTAAGGTCAG CCAGAACAAC CTGCCTGATG CCATCCAGGC CCCAAGTCTG
    GGGGGAAGAA AGGTCACTGT CACTTCTCTG GCTGGACAAC AGCCACCAGA AGGGGAGGGT
    GCATCTCCTG AAGAACAAAG GCTCATCCCT CGGACCCTTG GAGCCAAGAA GGGGCCCCCA
    GTACCAATAG AGAATGAGGA CTTCTGTGCT GTGTGCCTCA ATGGGGGCGA GCTACTGTGC
    TGTGACCGCT GCCCTAAGGT GTTCCACCTC TCCTGCCACC TGCCGGCCTT GCTTGGCTTC
    CCAGGGGGAG ATTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAT
    GACTGTGAGA ATGCCCGCTA TTACCAACCT GGGATGCGGG ACACCCCTGG CCTGAGCATC
    TATGACCAAA AGAAGTGTGA AAAGTTGGTT CTGTCCTTGT GCTGCAATAG CCTCAGCCTG
    CCCTTCCACG AGCCTGTCAG CCCGCTGGCC CGGCATTACT ACCAGATTAT CAAGAGGCCC
    ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTACACCACC
    CCAGAGGAGG TGGTGTCAGA CGTCCGCCTC ATGTTCTGGA ACTGTGCTAA ATTCAATTAT
    CCTGACTCAG AAGTTGCAGA GGCTGGCCGC TGCCTGGAAG TTTTCTTTGA GGGCTGGTTG
    AAGGAGATCT ATCCAGAAAA ACAGTTTGCC CAGCCGAGGC AGGAGGACTC AGACTCCGAG
    GAGGTGTCTA GCGAGAGTGG CTATTCCACT CCCCAGGGCT TCCCATGGCC TCCCTATGTG
    CAGGAGGGCA TCCAACCCAA GAGGCGGCGG CGACATATGG AGAATGAAAG AGCAAAAAGA
    ATGTCATTCC GCTTGGCCAG CAGCGTCTCC CAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005633694.1
    CDS39..4157
    Translation

    Target ORF information:

    RefSeq Version XM_005633637.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633637.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
    CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA
    AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC
    AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA
    AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC
    TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC
    TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG
    CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG
    AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG
    GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA
    CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA
    GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG
    GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT
    CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG
    CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC
    AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG
    GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC
    ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC
    AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG
    CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG
    CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG
    CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA
    AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG
    TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC
    CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA
    GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG
    GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC
    CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA
    TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA
    CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG
    CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA
    CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG
    CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG
    AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA
    TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA
    GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA
    TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG
    GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC
    TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG
    CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC
    GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC
    ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT
    CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG
    AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG
    CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG
    CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA
    GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT
    AAGGACCCCA CAACTCCTGG TCTGGAGGTT TCCAAGGATT TGGCTATCCC TTCAGAACTG
    GAGGAGCCAA TTAACCTCTC TGTGAAGAAA CCTCCAGTGG TCCCAGCAGT CAGCACAGCT
    ATGGCTCTGC AGCAGTACCA GAACCCAAAA GAGTATGAGA ATTTTGAAGA AGCCCTGGAG
    CTGAATGTAA AAGAGAACCA GAGTATCAGG CGAGAGCCTA AGATTCCCTA TGTGCGGCTG
    GAGCGGCTTA AGATCTGTGC TGCCTCCTCG GGAGAGATGC CTGTGTTCAA GCTGAAGCCC
    CAGAAGAATG AGCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC AGAGTCCTCC
    AGCATGTCCA TTAAGGTCAG CCAGAACAAC CTGCCTGATG CCATCCAGGC CCCAAGTCTG
    GGGGGAAGAA AGGTCACTGT CACTTCTCTG GCTGGACAAC AGCCACCAGA AGGGGAGGGT
    GCATCTCCTG AAGAACAAAG GCTCATCCCT CGGACCCTTG GAGCCAAGAA GGGGCCCCCA
    GTACCAATAG AGAATGAGGA CTTCTGTGCT GTGTGCCTCA ATGGGGGCGA GCTACTGTGC
    TGTGACCGCT GCCCTAAGGT GTTCCACCTC TCCTGCCACC TGCCGGCCTT GCTTGGCTTC
    CCAGGGGGAG ATTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAT
    GACTGTGAGA ATGCCCGCTA TTACCAACCT GGGATGCGGG ACACCCCTGG CCTGAGCATC
    TATGACCAAA AGAAGTGTGA AAAGTTGGTT CTGTCCTTGT GCTGCAATAG CCTCAGCCTG
    CCCTTCCACG AGCCTGTCAG CCCGCTGGCC CGGCATTACT ACCAGATTAT CAAGAGGCCC
    ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTACACCACC
    CCAGAGGAGG TGGTGTCAGA CGTCCGCCTC ATGTTCTGGA ACTGTGCTAA ATTCAATTAT
    CCTGACTCAG AAGTTGCAGA GGCTGGCCGC TGCCTGGAAG TTTTCTTTGA GGGCTGGTTG
    AAGGAGATCT ATCCAGAAAA ACAGTTTGCC CAGCCGAGGC AGGAGGACTC AGACTCCGAG
    GAGGTGTCTA GCGAGAGTGG CTATTCCACT CCCCAGGGCT TCCCATGGCC TCCCTATGTG
    CAGGAGGGCA TCCAACCCAA GAGGCGGCGG CGACATATGG AGAATGAAAG AGCAAAAAGA
    ATGTCATTCC GCTTGGCCAG CAGCGTCTCC CAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23169
    Clone ID Related Accession (Same CDS sequence) XM_005633637.2 , XM_005633637.3
    Accession Version XM_005633637.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tripartite motif-containing protein 66 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633637.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
    CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA
    AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC
    AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA
    AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC
    TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC
    TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG
    CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG
    AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG
    GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA
    CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA
    GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG
    GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT
    CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG
    CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC
    AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG
    GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC
    ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC
    AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG
    CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG
    CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG
    CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA
    AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG
    TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC
    CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA
    GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG
    GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC
    CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA
    TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA
    CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG
    CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA
    CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG
    CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG
    AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA
    TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA
    GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA
    TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG
    GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC
    TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG
    CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC
    GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC
    ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT
    CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG
    AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG
    CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG
    CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA
    GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT
    AAGGACCCCA CAACTCCTGG TCTGGAGGTT TCCAAGGATT TGGCTATCCC TTCAGAACTG
    GAGGAGCCAA TTAACCTCTC TGTGAAGAAA CCTCCAGTGG TCCCAGCAGT CAGCACAGCT
    ATGGCTCTGC AGCAGTACCA GAACCCAAAA GAGTATGAGA ATTTTGAAGA AGCCCTGGAG
    CTGAATGTAA AAGAGAACCA GAGTATCAGG CGAGAGCCTA AGATTCCCTA TGTGCGGCTG
    GAGCGGCTTA AGATCTGTGC TGCCTCCTCG GGAGAGATGC CTGTGTTCAA GCTGAAGCCC
    CAGAAGAATG AGCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC AGAGTCCTCC
    AGCATGTCCA TTAAGGTCAG CCAGAACAAC CTGCCTGATG CCATCCAGGC CCCAAGTCTG
    GGGGGAAGAA AGGTCACTGT CACTTCTCTG GCTGGACAAC AGCCACCAGA AGGGGAGGGT
    GCATCTCCTG AAGAACAAAG GCTCATCCCT CGGACCCTTG GAGCCAAGAA GGGGCCCCCA
    GTACCAATAG AGAATGAGGA CTTCTGTGCT GTGTGCCTCA ATGGGGGCGA GCTACTGTGC
    TGTGACCGCT GCCCTAAGGT GTTCCACCTC TCCTGCCACC TGCCGGCCTT GCTTGGCTTC
    CCAGGGGGAG ATTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAT
    GACTGTGAGA ATGCCCGCTA TTACCAACCT GGGATGCGGG ACACCCCTGG CCTGAGCATC
    TATGACCAAA AGAAGTGTGA AAAGTTGGTT CTGTCCTTGT GCTGCAATAG CCTCAGCCTG
    CCCTTCCACG AGCCTGTCAG CCCGCTGGCC CGGCATTACT ACCAGATTAT CAAGAGGCCC
    ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTACACCACC
    CCAGAGGAGG TGGTGTCAGA CGTCCGCCTC ATGTTCTGGA ACTGTGCTAA ATTCAATTAT
    CCTGACTCAG AAGTTGCAGA GGCTGGCCGC TGCCTGGAAG TTTTCTTTGA GGGCTGGTTG
    AAGGAGATCT ATCCAGAAAA ACAGTTTGCC CAGCCGAGGC AGGAGGACTC AGACTCCGAG
    GAGGTGTCTA GCGAGAGTGG CTATTCCACT CCCCAGGGCT TCCCATGGCC TCCCTATGTG
    CAGGAGGGCA TCCAACCCAA GAGGCGGCGG CGACATATGG AGAATGAAAG AGCAAAAAGA
    ATGTCATTCC GCTTGGCCAG CAGCGTCTCC CAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005633694.1
    CDS94..4212
    Translation

    Target ORF information:

    RefSeq Version XM_005633637.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005633637.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
    CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA
    AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC
    AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA
    AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC
    TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC
    TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG
    CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG
    AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG
    GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA
    CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA
    GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG
    GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT
    CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG
    CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC
    AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG
    GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC
    ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC
    AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG
    CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG
    CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG
    CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA
    AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG
    TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC
    CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA
    GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG
    GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC
    CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA
    TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA
    CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG
    CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA
    CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG
    CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG
    AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA
    TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA
    GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA
    TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG
    GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC
    TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG
    CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC
    GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC
    ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT
    CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG
    AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG
    CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG
    CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA
    GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT
    AAGGACCCCA CAACTCCTGG TCTGGAGGTT TCCAAGGATT TGGCTATCCC TTCAGAACTG
    GAGGAGCCAA TTAACCTCTC TGTGAAGAAA CCTCCAGTGG TCCCAGCAGT CAGCACAGCT
    ATGGCTCTGC AGCAGTACCA GAACCCAAAA GAGTATGAGA ATTTTGAAGA AGCCCTGGAG
    CTGAATGTAA AAGAGAACCA GAGTATCAGG CGAGAGCCTA AGATTCCCTA TGTGCGGCTG
    GAGCGGCTTA AGATCTGTGC TGCCTCCTCG GGAGAGATGC CTGTGTTCAA GCTGAAGCCC
    CAGAAGAATG AGCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC AGAGTCCTCC
    AGCATGTCCA TTAAGGTCAG CCAGAACAAC CTGCCTGATG CCATCCAGGC CCCAAGTCTG
    GGGGGAAGAA AGGTCACTGT CACTTCTCTG GCTGGACAAC AGCCACCAGA AGGGGAGGGT
    GCATCTCCTG AAGAACAAAG GCTCATCCCT CGGACCCTTG GAGCCAAGAA GGGGCCCCCA
    GTACCAATAG AGAATGAGGA CTTCTGTGCT GTGTGCCTCA ATGGGGGCGA GCTACTGTGC
    TGTGACCGCT GCCCTAAGGT GTTCCACCTC TCCTGCCACC TGCCGGCCTT GCTTGGCTTC
    CCAGGGGGAG ATTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAT
    GACTGTGAGA ATGCCCGCTA TTACCAACCT GGGATGCGGG ACACCCCTGG CCTGAGCATC
    TATGACCAAA AGAAGTGTGA AAAGTTGGTT CTGTCCTTGT GCTGCAATAG CCTCAGCCTG
    CCCTTCCACG AGCCTGTCAG CCCGCTGGCC CGGCATTACT ACCAGATTAT CAAGAGGCCC
    ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTACACCACC
    CCAGAGGAGG TGGTGTCAGA CGTCCGCCTC ATGTTCTGGA ACTGTGCTAA ATTCAATTAT
    CCTGACTCAG AAGTTGCAGA GGCTGGCCGC TGCCTGGAAG TTTTCTTTGA GGGCTGGTTG
    AAGGAGATCT ATCCAGAAAA ACAGTTTGCC CAGCCGAGGC AGGAGGACTC AGACTCCGAG
    GAGGTGTCTA GCGAGAGTGG CTATTCCACT CCCCAGGGCT TCCCATGGCC TCCCTATGTG
    CAGGAGGGCA TCCAACCCAA GAGGCGGCGG CGACATATGG AGAATGAAAG AGCAAAAAGA
    ATGTCATTCC GCTTGGCCAG CAGCGTCTCC CAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.