CACNA1D gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014122084.2	XP_013977559.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
XM_014122090.2	XP_013977565.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
XM_003432787.3	XP_003432835.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
XM_022406592.1	XP_022262300.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
XM_022406595.1	XP_022262303.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
XM_014122088.1	XP_013977563.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
XM_014122083.2	XP_013977558.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
XM_022406588.1	XP_022262296.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
XM_014122087.2	XP_013977562.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
XM_853570.4	XP_858663.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
XM_022406591.1	XP_022262299.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
XM_014122091.2	XP_013977566.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
XM_022406594.1	XP_022262302.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
XM_014122084.1	XP_013977559.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
XM_014122089.1	XP_013977564.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
XM_014122093.1	XP_013977568.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
XM_014122083.1	XP_013977558.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
XM_014122091.1	XP_013977566.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
XM_844338.4	XP_849431.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
XM_014122088.2	XP_013977563.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
XM_014122089.2	XP_013977564.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
XM_014122086.1	XP_013977561.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
XM_014122087.1	XP_013977562.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
XM_014122090.1	XP_013977565.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
XM_014122085.2	XP_013977560.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
XM_022406589.1	XP_022262297.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
XM_022406593.1	XP_022262301.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
XM_014122092.1	XP_013977567.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
XM_014122085.1	XP_013977560.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
XM_014122094.1	XP_013977569.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
XM_014122086.2	XP_013977561.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
XM_005632418.2	XP_005632475.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
XM_014122092.2	XP_013977567.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe41794	XM_014122091.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe41795	XM_014122092.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe21537	XM_844338.4 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe21538	XM_853570.4 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe21539	XM_005632418.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe21540	XM_003432787.3 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe295372	XM_022406588.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe295373	XM_022406589.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe295374	XM_022406591.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe295375	XM_022406592.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe295376	XM_022406593.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe295377	XM_022406594.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe295378	XM_022406595.1 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41786	XM_014122083.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41787	XM_014122084.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41788	XM_014122085.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41789	XM_014122086.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41790	XM_014122087.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41791	XM_014122088.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41792	XM_014122089.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41793	XM_014122090.2 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41796	XM_014122093.1 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41797	XM_014122094.1 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
OCe41787	XM_014122084.1	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41786	XM_014122083.1	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41794	XM_014122091.1	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe41791	XM_014122088.1	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41792	XM_014122089.1	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41790	XM_014122087.1	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41793	XM_014122090.1	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41788	XM_014122085.1	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41789	XM_014122086.1	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe41795	XM_014122092.1	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

CACNA1D ( XM_014122091.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977566.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OCe41794
Clone ID Related Accession (Same CDS sequence)	XM_014122091.2 , XM_014122091.1
Accession Version	XM_014122091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAACAGGAA ATTCGGGGTG TCATAGCCAT CATAACCATA 
ATTCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977566.1
CDS	312..5540
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKQEIRGVIAIITIIP

Target ORF information:

RefSeq Version	XM_014122091.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122091.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAACAGGAA ATTCGGGGTG TCATAGCCAT CATAACCATA 
ATTCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122091.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977566.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X13, mRNA.

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CloneID	OCe41794
Clone ID Related Accession (Same CDS sequence)	XM_014122091.2 , XM_014122091.1
Accession Version	XM_014122091.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAACAGGAA ATTCGGGGTG TCATAGCCAT CATAACCATA 
ATTCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977566.1
CDS	296..5524
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKQEIRGVIAIITIIP

Target ORF information:

RefSeq Version	XM_014122091.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122091.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAACAGGAA ATTCGGGGTG TCATAGCCAT CATAACCATA 
ATTCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122092.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977567.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X14, mRNA.

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CloneID	OCe41795
Clone ID Related Accession (Same CDS sequence)	XM_014122092.1 , XM_014122092.2
Accession Version	XM_014122092.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGATG CTTGAACGGA TGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977567.1
CDS	296..5383
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQMLERML

Target ORF information:

RefSeq Version	XM_014122092.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122092.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGATG CTTGAACGGA TGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122092.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977567.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X18, mRNA.

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CloneID	OCe41795
Clone ID Related Accession (Same CDS sequence)	XM_014122092.1 , XM_014122092.2
Accession Version	XM_014122092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGATG CTTGAACGGA TGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977567.1
CDS	312..5399
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQMLERML

Target ORF information:

RefSeq Version	XM_014122092.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122092.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGATG CTTGAACGGA TGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_844338.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_849431.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe21537
Clone ID Related Accession (Same CDS sequence)	XM_844338.4
Accession Version	XM_844338.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_844338.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_849431.1
CDS	1..6546
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_844338.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_844338.4

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_853570.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_858663.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X12, mRNA.

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CloneID	OCe21538
Clone ID Related Accession (Same CDS sequence)	XM_853570.4
Accession Version	XM_853570.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_853570.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_858663.1
CDS	1..6486
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_853570.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_853570.4

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_005632418.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005632475.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X13, mRNA.

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CloneID	OCe21539
Clone ID Related Accession (Same CDS sequence)	XM_005632418.2
Accession Version	XM_005632418.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005632418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005632475.1
CDS	1..6441
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_005632418.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632418.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_003432787.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432835.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X15, mRNA.

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CloneID	OCe21540
Clone ID Related Accession (Same CDS sequence)	XM_003432787.3
Accession Version	XM_003432787.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432787.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGATCTGAC TCGGGAGATG AGCAGTTCCC AACCATCTGC 
CGAGAGGATC CGGAGCCCCA CGGCTACTTC AGGGACGCCC GCTGCTTGGA GGAGCAGGAG 
TACTTCAGCG GCGAGGAGGG CTATGAGGAT GTGGGCTCTC CGGCCGGGGG CAGGCAGAGC 
TACAACTACT ACAACCGATA CCCGGGCAGC AGCTGGGACT TCGAGCGGCC CCGAGGCTAC 
CATCACCCCC AAGGCTTCTT GGAGGATGAC GACTCGCCCG TTTGCTATGA CTCCCGGAGA 
TCCCCAAGGA GACGCCTACT ACCTCCCACC CCGACATCGC ACCGGAGATC CTCCTTCAAC 
TTTGAGTGCC TGCGCCGGCA GAGCAGCCAG GAGGAGGTCT CGCTGTCCCC CACCTTCCCC 
CACCGCACAG CCCTGCCACT GCACCTGATG CAGCAACAGA TCATGGCAGT TGCGGGCCTG 
GATTCCAGTA AAGCCCAGAA GTACTCGCCG AGCCACTCAA CCCGGTCGTG GGCCACCCCT 
CCAGCGACCC CTCCGTACCG AGACTGGACG CCGTGCTACG CGCCCCTGAT CCAGGTGGAG 
CGGCCAGAGG CCCTGGACCA GGTCAACGGC AGCCTTCCAT CCCTGCACCG AAGCTCATGG 
TACACGGACG AGCCCGGCAT CTCATACCGA ACTTTCACAC CAGCCAGCCT GACTGTCCCC 
AGCAGCTTCC GCAACAAAAA CAGTGACAAG CAGAGGAGTG CAGACAGCTT GGTGGAGGCA 
GTCCTGATAT CCGAAGGCTT AGGACGGTAT GCAAGGGACC CAAAATTTGT ATCGGCAACA 
AAACACGAAA TCGCCGATGC CTGTGACCTG ACCATCGATG AGATGGAGAG CGCAGCCAGC 
AACCTGCTCA ATGGGACTGT GCATCCCCGG GCTAACGGGG GCGTGGGCCC TGTCTCACAC 
CGGCAGGACT ATGAGCTCCA GGACTTTGGG CCGGGCTACA GCGATGAGGA GCCTGACCTC 
GGAAGAGATG AGGAGGACCT GGCAGATGAA ATGATATGCA TCACCACCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432835.1
CDS	1..6414
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_003432787.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432787.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGATCTGAC TCGGGAGATG AGCAGTTCCC AACCATCTGC 
CGAGAGGATC CGGAGCCCCA CGGCTACTTC AGGGACGCCC GCTGCTTGGA GGAGCAGGAG 
TACTTCAGCG GCGAGGAGGG CTATGAGGAT GTGGGCTCTC CGGCCGGGGG CAGGCAGAGC 
TACAACTACT ACAACCGATA CCCGGGCAGC AGCTGGGACT TCGAGCGGCC CCGAGGCTAC 
CATCACCCCC AAGGCTTCTT GGAGGATGAC GACTCGCCCG TTTGCTATGA CTCCCGGAGA 
TCCCCAAGGA GACGCCTACT ACCTCCCACC CCGACATCGC ACCGGAGATC CTCCTTCAAC 
TTTGAGTGCC TGCGCCGGCA GAGCAGCCAG GAGGAGGTCT CGCTGTCCCC CACCTTCCCC 
CACCGCACAG CCCTGCCACT GCACCTGATG CAGCAACAGA TCATGGCAGT TGCGGGCCTG 
GATTCCAGTA AAGCCCAGAA GTACTCGCCG AGCCACTCAA CCCGGTCGTG GGCCACCCCT 
CCAGCGACCC CTCCGTACCG AGACTGGACG CCGTGCTACG CGCCCCTGAT CCAGGTGGAG 
CGGCCAGAGG CCCTGGACCA GGTCAACGGC AGCCTTCCAT CCCTGCACCG AAGCTCATGG 
TACACGGACG AGCCCGGCAT CTCATACCGA ACTTTCACAC CAGCCAGCCT GACTGTCCCC 
AGCAGCTTCC GCAACAAAAA CAGTGACAAG CAGAGGAGTG CAGACAGCTT GGTGGAGGCA 
GTCCTGATAT CCGAAGGCTT AGGACGGTAT GCAAGGGACC CAAAATTTGT ATCGGCAACA 
AAACACGAAA TCGCCGATGC CTGTGACCTG ACCATCGATG AGATGGAGAG CGCAGCCAGC 
AACCTGCTCA ATGGGACTGT GCATCCCCGG GCTAACGGGG GCGTGGGCCC TGTCTCACAC 
CGGCAGGACT ATGAGCTCCA GGACTTTGGG CCGGGCTACA GCGATGAGGA GCCTGACCTC 
GGAAGAGATG AGGAGGACCT GGCAGATGAA ATGATATGCA TCACCACCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_022406588.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262296.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.

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CloneID	OCe295372
Clone ID Related Accession (Same CDS sequence)	XM_022406588.1
Accession Version	XM_022406588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACGTTGCTGT GGACTTTTAT AAAGTCCTTT 
CAGGCTCTGC CCTATGTCGC CCTCCTCATA GCCATGCTGT TCTTCATCTA CGCGGTTATC 
GGCATGCAGA TGTTTGGAAA GGTTGCTATG AGAGATAACA ACCAGATCAA CAGGAACAAC 
AATTTCCAGA CCTTTCCTCA GGCGGTGCTG CTACTCTTCA GGTGTGCCAC GGGTGAAGCC 
TGGCAGGAGA TCATGCTGGC CTGTCTCCCG GGGAAGCTCT GTGACCCCGA GTCAGACTAT 
AACCCTGGGG AGGAGTACAC ATGTGGGAGC AACTTTGCCA TTGTTTATTT CATCAGTTTT 
TACATGCTCT GCGCATTTCT GATCATCAAC CTGTTTGTGG CTGTCATCAT GGACAACTTT 
GATTATCTGA CTCGGGACTG GTCCATTCTA GGGCCTCACC ATTTAGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAGGCAAAA GGAAGGATCA AGCACCTTGA TGTGGTCACT 
CTGCTCCGAC GCATCCAGCC TCCCCTGGGG TTTGGGAAAT TATGCCCACA CAGAGTAGCC 
TGTAAGAGAC TAGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTTAAT 
GCAACCCTGT TTGCTTTGGT TCGAACGGCT CTTAAAATCA AGACAGAAGG GAACCTGGAA 
CAAGCTAATG AGGAACTCCG AGCTGTGATA AAGAAAATCT GGAAGAAAAC CAGCATGAAA 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA ACAAGGACTG 
GTGGGAAAAT ACCCTGCGAA GAACACCACC ATTGCCCTAC AGGCAGGATT AAGGACACTG 
CATGACATTG GGCCAGAAAT CCGGCGTGCT ATATCATGTG ATTTGCAAGA TGACGAGCCG 
GAGGAAACAA AACGAGAAGA AGAAGAAGAT GGATTCAAAA GAAATGGTGC CCTGCTTGGA 
AACCATGTCA ATCATGTTAA TAGTGATAGG AGAGACCCCC TTCAGCAGAC CAATACCACC 
CACCGTCCCC TGCATGTCCA AAGGCCTTCA GTTCCACCTG CAAGTGATAC TGAGAGACCG 
CTGTTTCCTC CAGCAGGAAA TTCGGGGTGT CATAGCCATC ATAACCATAA TTCCGTAGGA 
AAGCAAGCTC CCAGCTCAAC AAATGCCAAT CTCAATAATG CCAATATGTC CAAAGCTGCC 
CCTGGAAAGC GGCCCAGCAT TGGGAACCTT GAGCATATGT CTGAAAATGG GCATCACTCC 
TCCCACAAGC ATGACCGGGA GCCTCAAAGA AGGTCCAGTA TTAAAAGAAC CCGCTATTAT 
GAAACTTACA TTAGATCTGA CTCGGGAGAT GAGCAGTTCC CAACCATCTG CCGAGAGGAT 
CCGGAGCCCC ACGGCTACTT CAGGGACGCC CGCTGCTTGG AGGAGCAGGA GTACTTCAGC 
GGCGAGGAGG GCTATGAGGA TGTGGGCTCT CCGGCCGGGG GCAGGCAGAG CTACAACTAC 
TACAACCGAT ACCCGGGCAG CAGCTGGGAC TTCGAGCGGC CCCGAGGCTA CCATCACCCC 
CAAGGCTTCT TGGAGGATGA CGACTCGCCC GTTTGCTATG ACTCCCGGAG ATCCCCAAGG 
AGACGCCTAC TACCTCCCAC CCCGACATCG CACCGGAGAT CCTCCTTCAA CTTTGAGTGC 
CTGCGCCGGC AGAGCAGCCA GGAGGAGGTC TCGCTGTCCC CCACCTTCCC CCACCGCACA 
GCCCTGCCAC TGCACCTGAT GCAGCAACAG ATCATGGCAG TTGCGGGCCT GGATTCCAGT 
AAAGCCCAGA AGTACTCGCC GAGCCACTCA ACCCGGTCGT GGGCCACCCC TCCAGCGACC 
CCTCCGTACC GAGACTGGAC GCCGTGCTAC GCGCCCCTGA TCCAGGTGGA GCGGCCAGAG 
GCCCTGGACC AGGTCAACGG CAGCCTTCCA TCCCTGCACC GAAGCTCATG GTACACGGAC 
GAGCCCGGCA TCTCATACCG AACTTTCACA CCAGCCAGCC TGACTGTCCC CAGCAGCTTC 
CGCAACAAAA ACAGTGACAA GCAGAGGAGT GCAGACAGCT TGGTGGAGGC AGTCCTGATA 
TCCGAAGGCT TAGGACGGTA TGCAAGGGAC CCAAAATTTG TATCGGCAAC AAAACACGAA 
ATCGCCGATG CCTGTGACCT GACCATCGAT GAGATGGAGA GCGCAGCCAG CAACCTGCTC 
AATGGGACTG TGCATCCCCG GGCTAACGGG GGCGTGGGCC CTGTCTCACA CCGGCAGGAC 
TATGAGCTCC AGGACTTTGG GCCGGGCTAC AGCGATGAGG AGCCTGACCT CGGAAGAGAT 
GAGGAGGACC TGGCAGATGA AATGATATGC ATCACCACCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262296.1
CDS	308..6892
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_022406588.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406588.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACGTTGCTGT GGACTTTTAT AAAGTCCTTT 
CAGGCTCTGC CCTATGTCGC CCTCCTCATA GCCATGCTGT TCTTCATCTA CGCGGTTATC 
GGCATGCAGA TGTTTGGAAA GGTTGCTATG AGAGATAACA ACCAGATCAA CAGGAACAAC 
AATTTCCAGA CCTTTCCTCA GGCGGTGCTG CTACTCTTCA GGTGTGCCAC GGGTGAAGCC 
TGGCAGGAGA TCATGCTGGC CTGTCTCCCG GGGAAGCTCT GTGACCCCGA GTCAGACTAT 
AACCCTGGGG AGGAGTACAC ATGTGGGAGC AACTTTGCCA TTGTTTATTT CATCAGTTTT 
TACATGCTCT GCGCATTTCT GATCATCAAC CTGTTTGTGG CTGTCATCAT GGACAACTTT 
GATTATCTGA CTCGGGACTG GTCCATTCTA GGGCCTCACC ATTTAGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAGGCAAAA GGAAGGATCA AGCACCTTGA TGTGGTCACT 
CTGCTCCGAC GCATCCAGCC TCCCCTGGGG TTTGGGAAAT TATGCCCACA CAGAGTAGCC 
TGTAAGAGAC TAGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTTAAT 
GCAACCCTGT TTGCTTTGGT TCGAACGGCT CTTAAAATCA AGACAGAAGG GAACCTGGAA 
CAAGCTAATG AGGAACTCCG AGCTGTGATA AAGAAAATCT GGAAGAAAAC CAGCATGAAA 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA ACAAGGACTG 
GTGGGAAAAT ACCCTGCGAA GAACACCACC ATTGCCCTAC AGGCAGGATT AAGGACACTG 
CATGACATTG GGCCAGAAAT CCGGCGTGCT ATATCATGTG ATTTGCAAGA TGACGAGCCG 
GAGGAAACAA AACGAGAAGA AGAAGAAGAT GGATTCAAAA GAAATGGTGC CCTGCTTGGA 
AACCATGTCA ATCATGTTAA TAGTGATAGG AGAGACCCCC TTCAGCAGAC CAATACCACC 
CACCGTCCCC TGCATGTCCA AAGGCCTTCA GTTCCACCTG CAAGTGATAC TGAGAGACCG 
CTGTTTCCTC CAGCAGGAAA TTCGGGGTGT CATAGCCATC ATAACCATAA TTCCGTAGGA 
AAGCAAGCTC CCAGCTCAAC AAATGCCAAT CTCAATAATG CCAATATGTC CAAAGCTGCC 
CCTGGAAAGC GGCCCAGCAT TGGGAACCTT GAGCATATGT CTGAAAATGG GCATCACTCC 
TCCCACAAGC ATGACCGGGA GCCTCAAAGA AGGTCCAGTA TTAAAAGAAC CCGCTATTAT 
GAAACTTACA TTAGATCTGA CTCGGGAGAT GAGCAGTTCC CAACCATCTG CCGAGAGGAT 
CCGGAGCCCC ACGGCTACTT CAGGGACGCC CGCTGCTTGG AGGAGCAGGA GTACTTCAGC 
GGCGAGGAGG GCTATGAGGA TGTGGGCTCT CCGGCCGGGG GCAGGCAGAG CTACAACTAC 
TACAACCGAT ACCCGGGCAG CAGCTGGGAC TTCGAGCGGC CCCGAGGCTA CCATCACCCC 
CAAGGCTTCT TGGAGGATGA CGACTCGCCC GTTTGCTATG ACTCCCGGAG ATCCCCAAGG 
AGACGCCTAC TACCTCCCAC CCCGACATCG CACCGGAGAT CCTCCTTCAA CTTTGAGTGC 
CTGCGCCGGC AGAGCAGCCA GGAGGAGGTC TCGCTGTCCC CCACCTTCCC CCACCGCACA 
GCCCTGCCAC TGCACCTGAT GCAGCAACAG ATCATGGCAG TTGCGGGCCT GGATTCCAGT 
AAAGCCCAGA AGTACTCGCC GAGCCACTCA ACCCGGTCGT GGGCCACCCC TCCAGCGACC 
CCTCCGTACC GAGACTGGAC GCCGTGCTAC GCGCCCCTGA TCCAGGTGGA GCGGCCAGAG 
GCCCTGGACC AGGTCAACGG CAGCCTTCCA TCCCTGCACC GAAGCTCATG GTACACGGAC 
GAGCCCGGCA TCTCATACCG AACTTTCACA CCAGCCAGCC TGACTGTCCC CAGCAGCTTC 
CGCAACAAAA ACAGTGACAA GCAGAGGAGT GCAGACAGCT TGGTGGAGGC AGTCCTGATA 
TCCGAAGGCT TAGGACGGTA TGCAAGGGAC CCAAAATTTG TATCGGCAAC AAAACACGAA 
ATCGCCGATG CCTGTGACCT GACCATCGAT GAGATGGAGA GCGCAGCCAG CAACCTGCTC 
AATGGGACTG TGCATCCCCG GGCTAACGGG GGCGTGGGCC CTGTCTCACA CCGGCAGGAC 
TATGAGCTCC AGGACTTTGG GCCGGGCTAC AGCGATGAGG AGCCTGACCT CGGAAGAGAT 
GAGGAGGACC TGGCAGATGA AATGATATGC ATCACCACCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_022406589.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262297.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X11, mRNA.

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CloneID	OCe295373
Clone ID Related Accession (Same CDS sequence)	XM_022406589.1
Accession Version	XM_022406589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262297.1
CDS	308..6808
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_022406589.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406589.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_022406591.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262299.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X14, mRNA.

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CloneID	OCe295374
Clone ID Related Accession (Same CDS sequence)	XM_022406591.1
Accession Version	XM_022406591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGGTTGG TGAAACTTCT CAGCAGGGGG GAAGGCATCC GGACGTTGCT GTGGACTTTT 
ATAAAGTCCT TTCAGGCTCT GCCCTATGTC GCCCTCCTCA TAGCCATGCT GTTCTTCATC 
TACGCGGTTA TCGGCATGCA GATGTTTGGA AAGGTTGCTA TGAGAGATAA CAACCAGATC 
AACAGGAACA ACAATTTCCA GACCTTTCCT CAGGCGGTGC TGCTACTCTT CAGGTGTGCC 
ACGGGTGAAG CCTGGCAGGA GATCATGCTG GCCTGTCTCC CGGGGAAGCT CTGTGACCCC 
GAGTCAGACT ATAACCCTGG GGAGGAGTAC ACATGTGGGA GCAACTTTGC CATTGTTTAT 
TTCATCAGTT TTTACATGCT CTGCGCATTT CTGATCATCA ACCTGTTTGT GGCTGTCATC 
ATGGACAACT TTGATTATCT GACTCGGGAC TGGTCCATTC TAGGGCCTCA CCATTTAGAC 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAGGCAA AAGGAAGGAT CAAGCACCTT 
GATGTGGTCA CTCTGCTCCG ACGCATCCAG CCTCCCCTGG GGTTTGGGAA ATTATGCCCA 
CACAGAGTAG CCTGTAAGAG ACTAGTTGCC ATGAACATGC CTCTCAACAG TGATGGGACA 
GTCATGTTTA ATGCAACCCT GTTTGCTTTG GTTCGAACGG CTCTTAAAAT CAAGACAGAA 
GGGAACCTGG AACAAGCTAA TGAGGAACTC CGAGCTGTGA TAAAGAAAAT CTGGAAGAAA 
ACCAGCATGA AACTGCTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA ATACCCTGCG AAGAACACCA CCATTGCCCT ACAGGCAGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCATG TGATTTGCAA 
GATGACGAGC CGGAGGAAAC AAAACGAGAA GAAGAAGAAG ATGGATTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGACCC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAGTTCCACC TGCAAGTGAT 
ACTGAGAGAC CGCTGTTTCC TCCAGCAGGA AATTCGGGGT GTCATAGCCA TCATAACCAT 
AATTCCGTAG GAAAGCAAGC TCCCAGCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TCCAAAGCTG CCCCTGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCATAT GTCTGAAAAT 
GGGCATCACT CCTCCCACAA GCATGACCGG GAGCCTCAAA GAAGGTCCAG TATTAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGATCT GACTCGGGAG ATGAGCAGTT CCCAACCATC 
TGCCGAGAGG ATCCGGAGCC CCACGGCTAC TTCAGGGACG CCCGCTGCTT GGAGGAGCAG 
GAGTACTTCA GCGGCGAGGA GGGCTATGAG GATGTGGGCT CTCCGGCCGG GGGCAGGCAG 
AGCTACAACT ACTACAACCG ATACCCGGGC AGCAGCTGGG ACTTCGAGCG GCCCCGAGGC 
TACCATCACC CCCAAGGCTT CTTGGAGGAT GACGACTCGC CCGTTTGCTA TGACTCCCGG 
AGATCCCCAA GGAGACGCCT ACTACCTCCC ACCCCGACAT CGCACCGGAG ATCCTCCTTC 
AACTTTGAGT GCCTGCGCCG GCAGAGCAGC CAGGAGGAGG TCTCGCTGTC CCCCACCTTC 
CCCCACCGCA CAGCCCTGCC ACTGCACCTG ATGCAGCAAC AGATCATGGC AGTTGCGGGC 
CTGGATTCCA GTAAAGCCCA GAAGTACTCG CCGAGCCACT CAACCCGGTC GTGGGCCACC 
CCTCCAGCGA CCCCTCCGTA CCGAGACTGG ACGCCGTGCT ACGCGCCCCT GATCCAGGTG 
GAGCGGCCAG AGGCCCTGGA CCAGGTCAAC GGCAGCCTTC CATCCCTGCA CCGAAGCTCA 
TGGTACACGG ACGAGCCCGG CATCTCATAC CGAACTTTCA CACCAGCCAG CCTGACTGTC 
CCCAGCAGCT TCCGCAACAA AAACAGTGAC AAGCAGAGGA GTGCAGACAG CTTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTAGGACGG TATGCAAGGG ACCCAAAATT TGTATCGGCA 
ACAAAACACG AAATCGCCGA TGCCTGTGAC CTGACCATCG ATGAGATGGA GAGCGCAGCC 
AGCAACCTGC TCAATGGGAC TGTGCATCCC CGGGCTAACG GGGGCGTGGG CCCTGTCTCA 
CACCGGCAGG ACTATGAGCT CCAGGACTTT GGGCCGGGCT ACAGCGATGA GGAGCCTGAC 
CTCGGAAGAG ATGAGGAGGA CCTGGCAGAT GAAATGATAT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262299.1
CDS	308..6724
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_022406591.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406591.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGGTTGG TGAAACTTCT CAGCAGGGGG GAAGGCATCC GGACGTTGCT GTGGACTTTT 
ATAAAGTCCT TTCAGGCTCT GCCCTATGTC GCCCTCCTCA TAGCCATGCT GTTCTTCATC 
TACGCGGTTA TCGGCATGCA GATGTTTGGA AAGGTTGCTA TGAGAGATAA CAACCAGATC 
AACAGGAACA ACAATTTCCA GACCTTTCCT CAGGCGGTGC TGCTACTCTT CAGGTGTGCC 
ACGGGTGAAG CCTGGCAGGA GATCATGCTG GCCTGTCTCC CGGGGAAGCT CTGTGACCCC 
GAGTCAGACT ATAACCCTGG GGAGGAGTAC ACATGTGGGA GCAACTTTGC CATTGTTTAT 
TTCATCAGTT TTTACATGCT CTGCGCATTT CTGATCATCA ACCTGTTTGT GGCTGTCATC 
ATGGACAACT TTGATTATCT GACTCGGGAC TGGTCCATTC TAGGGCCTCA CCATTTAGAC 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAGGCAA AAGGAAGGAT CAAGCACCTT 
GATGTGGTCA CTCTGCTCCG ACGCATCCAG CCTCCCCTGG GGTTTGGGAA ATTATGCCCA 
CACAGAGTAG CCTGTAAGAG ACTAGTTGCC ATGAACATGC CTCTCAACAG TGATGGGACA 
GTCATGTTTA ATGCAACCCT GTTTGCTTTG GTTCGAACGG CTCTTAAAAT CAAGACAGAA 
GGGAACCTGG AACAAGCTAA TGAGGAACTC CGAGCTGTGA TAAAGAAAAT CTGGAAGAAA 
ACCAGCATGA AACTGCTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA ATACCCTGCG AAGAACACCA CCATTGCCCT ACAGGCAGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCATG TGATTTGCAA 
GATGACGAGC CGGAGGAAAC AAAACGAGAA GAAGAAGAAG ATGGATTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGACCC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAGTTCCACC TGCAAGTGAT 
ACTGAGAGAC CGCTGTTTCC TCCAGCAGGA AATTCGGGGT GTCATAGCCA TCATAACCAT 
AATTCCGTAG GAAAGCAAGC TCCCAGCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TCCAAAGCTG CCCCTGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCATAT GTCTGAAAAT 
GGGCATCACT CCTCCCACAA GCATGACCGG GAGCCTCAAA GAAGGTCCAG TATTAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGATCT GACTCGGGAG ATGAGCAGTT CCCAACCATC 
TGCCGAGAGG ATCCGGAGCC CCACGGCTAC TTCAGGGACG CCCGCTGCTT GGAGGAGCAG 
GAGTACTTCA GCGGCGAGGA GGGCTATGAG GATGTGGGCT CTCCGGCCGG GGGCAGGCAG 
AGCTACAACT ACTACAACCG ATACCCGGGC AGCAGCTGGG ACTTCGAGCG GCCCCGAGGC 
TACCATCACC CCCAAGGCTT CTTGGAGGAT GACGACTCGC CCGTTTGCTA TGACTCCCGG 
AGATCCCCAA GGAGACGCCT ACTACCTCCC ACCCCGACAT CGCACCGGAG ATCCTCCTTC 
AACTTTGAGT GCCTGCGCCG GCAGAGCAGC CAGGAGGAGG TCTCGCTGTC CCCCACCTTC 
CCCCACCGCA CAGCCCTGCC ACTGCACCTG ATGCAGCAAC AGATCATGGC AGTTGCGGGC 
CTGGATTCCA GTAAAGCCCA GAAGTACTCG CCGAGCCACT CAACCCGGTC GTGGGCCACC 
CCTCCAGCGA CCCCTCCGTA CCGAGACTGG ACGCCGTGCT ACGCGCCCCT GATCCAGGTG 
GAGCGGCCAG AGGCCCTGGA CCAGGTCAAC GGCAGCCTTC CATCCCTGCA CCGAAGCTCA 
TGGTACACGG ACGAGCCCGG CATCTCATAC CGAACTTTCA CACCAGCCAG CCTGACTGTC 
CCCAGCAGCT TCCGCAACAA AAACAGTGAC AAGCAGAGGA GTGCAGACAG CTTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTAGGACGG TATGCAAGGG ACCCAAAATT TGTATCGGCA 
ACAAAACACG AAATCGCCGA TGCCTGTGAC CTGACCATCG ATGAGATGGA GAGCGCAGCC 
AGCAACCTGC TCAATGGGAC TGTGCATCCC CGGGCTAACG GGGGCGTGGG CCCTGTCTCA 
CACCGGCAGG ACTATGAGCT CCAGGACTTT GGGCCGGGCT ACAGCGATGA GGAGCCTGAC 
CTCGGAAGAG ATGAGGAGGA CCTGGCAGAT GAAATGATAT GCATCACCAC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_022406592.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262300.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X16, mRNA.

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CloneID	OCe295375
Clone ID Related Accession (Same CDS sequence)	XM_022406592.1
Accession Version	XM_022406592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGCTC TGCCCTATGT CGCCCTCCTC ATAGCCATGC TGTTCTTCAT CTACGCGGTT 
ATCGGCATGC AGATGTTTGG AAAGGTTGCT ATGAGAGATA ACAACCAGAT CAACAGGAAC 
AACAATTTCC AGACCTTTCC TCAGGCGGTG CTGCTACTCT TCAGGTGTGC CACGGGTGAA 
GCCTGGCAGG AGATCATGCT GGCCTGTCTC CCGGGGAAGC TCTGTGACCC CGAGTCAGAC 
TATAACCCTG GGGAGGAGTA CACATGTGGG AGCAACTTTG CCATTGTTTA TTTCATCAGT 
TTTTACATGC TCTGCGCATT TCTGATCATC AACCTGTTTG TGGCTGTCAT CATGGACAAC 
TTTGATTATC TGACTCGGGA CTGGTCCATT CTAGGGCCTC ACCATTTAGA CGAATTCAAA 
AGAATATGGT CAGAATATGA CCCTGAGGCA AAAGGAAGGA TCAAGCACCT TGATGTGGTC 
ACTCTGCTCC GACGCATCCA GCCTCCCCTG GGGTTTGGGA AATTATGCCC ACACAGAGTA 
GCCTGTAAGA GACTAGTTGC CATGAACATG CCTCTCAACA GTGATGGGAC AGTCATGTTT 
AATGCAACCC TGTTTGCTTT GGTTCGAACG GCTCTTAAAA TCAAGACAGA AGGGAACCTG 
GAACAAGCTA ATGAGGAACT CCGAGCTGTG ATAAAGAAAA TCTGGAAGAA AACCAGCATG 
AAACTGCTTG ACCAAGTTGT CCCTCCAGCT GGTGATGATG AGGTAACCGT GGGGAAGTTC 
TATGCCACTT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGGAA AGAACAAGGA 
CTGGTGGGAA AATACCCTGC GAAGAACACC ACCATTGCCC TACAGGCAGG ATTAAGGACA 
CTGCATGACA TTGGGCCAGA AATCCGGCGT GCTATATCAT GTGATTTGCA AGATGACGAG 
CCGGAGGAAA CAAAACGAGA AGAAGAAGAA GATGGATTCA AAAGAAATGG TGCCCTGCTT 
GGAAACCATG TCAATCATGT TAATAGTGAT AGGAGAGACC CCCTTCAGCA GACCAATACC 
ACCCACCGTC CCCTGCATGT CCAAAGGCCT TCAGTTCCAC CTGCAAGTGA TACTGAGAGA 
CCGCTGTTTC CTCCAGCAGG AAATTCGGGG TGTCATAGCC ATCATAACCA TAATTCCGTA 
GGAAAGCAAG CTCCCAGCTC AACAAATGCC AATCTCAATA ATGCCAATAT GTCCAAAGCT 
GCCCCTGGAA AGCGGCCCAG CATTGGGAAC CTTGAGCATA TGTCTGAAAA TGGGCATCAC 
TCCTCCCACA AGCATGACCG GGAGCCTCAA AGAAGGTCCA GTATTAAAAG AACCCGCTAT 
TATGAAACTT ACATTAGATC TGACTCGGGA GATGAGCAGT TCCCAACCAT CTGCCGAGAG 
GATCCGGAGC CCCACGGCTA CTTCAGGGAC GCCCGCTGCT TGGAGGAGCA GGAGTACTTC 
AGCGGCGAGG AGGGCTATGA GGATGTGGGC TCTCCGGCCG GGGGCAGGCA GAGCTACAAC 
TACTACAACC GATACCCGGG CAGCAGCTGG GACTTCGAGC GGCCCCGAGG CTACCATCAC 
CCCCAAGGCT TCTTGGAGGA TGACGACTCG CCCGTTTGCT ATGACTCCCG GAGATCCCCA 
AGGAGACGCC TACTACCTCC CACCCCGACA TCGCACCGGA GATCCTCCTT CAACTTTGAG 
TGCCTGCGCC GGCAGAGCAG CCAGGAGGAG GTCTCGCTGT CCCCCACCTT CCCCCACCGC 
ACAGCCCTGC CACTGCACCT GATGCAGCAA CAGATCATGG CAGTTGCGGG CCTGGATTCC 
AGTAAAGCCC AGAAGTACTC GCCGAGCCAC TCAACCCGGT CGTGGGCCAC CCCTCCAGCG 
ACCCCTCCGT ACCGAGACTG GACGCCGTGC TACGCGCCCC TGATCCAGGT GGAGCGGCCA 
GAGGCCCTGG ACCAGGTCAA CGGCAGCCTT CCATCCCTGC ACCGAAGCTC ATGGTACACG 
GACGAGCCCG GCATCTCATA CCGAACTTTC ACACCAGCCA GCCTGACTGT CCCCAGCAGC 
TTCCGCAACA AAAACAGTGA CAAGCAGAGG AGTGCAGACA GCTTGGTGGA GGCAGTCCTG 
ATATCCGAAG GCTTAGGACG GTATGCAAGG GACCCAAAAT TTGTATCGGC AACAAAACAC 
GAAATCGCCG ATGCCTGTGA CCTGACCATC GATGAGATGG AGAGCGCAGC CAGCAACCTG 
CTCAATGGGA CTGTGCATCC CCGGGCTAAC GGGGGCGTGG GCCCTGTCTC ACACCGGCAG 
GACTATGAGC TCCAGGACTT TGGGCCGGGC TACAGCGATG AGGAGCCTGA CCTCGGAAGA 
GATGAGGAGG ACCTGGCAGA TGAAATGATA TGCATCACCA CCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262300.1
CDS	308..6595
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_022406592.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406592.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGCTC TGCCCTATGT CGCCCTCCTC ATAGCCATGC TGTTCTTCAT CTACGCGGTT 
ATCGGCATGC AGATGTTTGG AAAGGTTGCT ATGAGAGATA ACAACCAGAT CAACAGGAAC 
AACAATTTCC AGACCTTTCC TCAGGCGGTG CTGCTACTCT TCAGGTGTGC CACGGGTGAA 
GCCTGGCAGG AGATCATGCT GGCCTGTCTC CCGGGGAAGC TCTGTGACCC CGAGTCAGAC 
TATAACCCTG GGGAGGAGTA CACATGTGGG AGCAACTTTG CCATTGTTTA TTTCATCAGT 
TTTTACATGC TCTGCGCATT TCTGATCATC AACCTGTTTG TGGCTGTCAT CATGGACAAC 
TTTGATTATC TGACTCGGGA CTGGTCCATT CTAGGGCCTC ACCATTTAGA CGAATTCAAA 
AGAATATGGT CAGAATATGA CCCTGAGGCA AAAGGAAGGA TCAAGCACCT TGATGTGGTC 
ACTCTGCTCC GACGCATCCA GCCTCCCCTG GGGTTTGGGA AATTATGCCC ACACAGAGTA 
GCCTGTAAGA GACTAGTTGC CATGAACATG CCTCTCAACA GTGATGGGAC AGTCATGTTT 
AATGCAACCC TGTTTGCTTT GGTTCGAACG GCTCTTAAAA TCAAGACAGA AGGGAACCTG 
GAACAAGCTA ATGAGGAACT CCGAGCTGTG ATAAAGAAAA TCTGGAAGAA AACCAGCATG 
AAACTGCTTG ACCAAGTTGT CCCTCCAGCT GGTGATGATG AGGTAACCGT GGGGAAGTTC 
TATGCCACTT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGGAA AGAACAAGGA 
CTGGTGGGAA AATACCCTGC GAAGAACACC ACCATTGCCC TACAGGCAGG ATTAAGGACA 
CTGCATGACA TTGGGCCAGA AATCCGGCGT GCTATATCAT GTGATTTGCA AGATGACGAG 
CCGGAGGAAA CAAAACGAGA AGAAGAAGAA GATGGATTCA AAAGAAATGG TGCCCTGCTT 
GGAAACCATG TCAATCATGT TAATAGTGAT AGGAGAGACC CCCTTCAGCA GACCAATACC 
ACCCACCGTC CCCTGCATGT CCAAAGGCCT TCAGTTCCAC CTGCAAGTGA TACTGAGAGA 
CCGCTGTTTC CTCCAGCAGG AAATTCGGGG TGTCATAGCC ATCATAACCA TAATTCCGTA 
GGAAAGCAAG CTCCCAGCTC AACAAATGCC AATCTCAATA ATGCCAATAT GTCCAAAGCT 
GCCCCTGGAA AGCGGCCCAG CATTGGGAAC CTTGAGCATA TGTCTGAAAA TGGGCATCAC 
TCCTCCCACA AGCATGACCG GGAGCCTCAA AGAAGGTCCA GTATTAAAAG AACCCGCTAT 
TATGAAACTT ACATTAGATC TGACTCGGGA GATGAGCAGT TCCCAACCAT CTGCCGAGAG 
GATCCGGAGC CCCACGGCTA CTTCAGGGAC GCCCGCTGCT TGGAGGAGCA GGAGTACTTC 
AGCGGCGAGG AGGGCTATGA GGATGTGGGC TCTCCGGCCG GGGGCAGGCA GAGCTACAAC 
TACTACAACC GATACCCGGG CAGCAGCTGG GACTTCGAGC GGCCCCGAGG CTACCATCAC 
CCCCAAGGCT TCTTGGAGGA TGACGACTCG CCCGTTTGCT ATGACTCCCG GAGATCCCCA 
AGGAGACGCC TACTACCTCC CACCCCGACA TCGCACCGGA GATCCTCCTT CAACTTTGAG 
TGCCTGCGCC GGCAGAGCAG CCAGGAGGAG GTCTCGCTGT CCCCCACCTT CCCCCACCGC 
ACAGCCCTGC CACTGCACCT GATGCAGCAA CAGATCATGG CAGTTGCGGG CCTGGATTCC 
AGTAAAGCCC AGAAGTACTC GCCGAGCCAC TCAACCCGGT CGTGGGCCAC CCCTCCAGCG 
ACCCCTCCGT ACCGAGACTG GACGCCGTGC TACGCGCCCC TGATCCAGGT GGAGCGGCCA 
GAGGCCCTGG ACCAGGTCAA CGGCAGCCTT CCATCCCTGC ACCGAAGCTC ATGGTACACG 
GACGAGCCCG GCATCTCATA CCGAACTTTC ACACCAGCCA GCCTGACTGT CCCCAGCAGC 
TTCCGCAACA AAAACAGTGA CAAGCAGAGG AGTGCAGACA GCTTGGTGGA GGCAGTCCTG 
ATATCCGAAG GCTTAGGACG GTATGCAAGG GACCCAAAAT TTGTATCGGC AACAAAACAC 
GAAATCGCCG ATGCCTGTGA CCTGACCATC GATGAGATGG AGAGCGCAGC CAGCAACCTG 
CTCAATGGGA CTGTGCATCC CCGGGCTAAC GGGGGCGTGG GCCCTGTCTC ACACCGGCAG 
GACTATGAGC TCCAGGACTT TGGGCCGGGC TACAGCGATG AGGAGCCTGA CCTCGGAAGA 
GATGAGGAGG ACCTGGCAGA TGAAATGATA TGCATCACCA CCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_022406593.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262301.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X19, mRNA.

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CloneID	OCe295376
Clone ID Related Accession (Same CDS sequence)	XM_022406593.1
Accession Version	XM_022406593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACGTTGCTGT GGACTTTTAT AAAGTCCTTT 
CAGGCTCTGC CCTATGTCGC CCTCCTCATA GCCATGCTGT TCTTCATCTA CGCGGTTATC 
GGCATGCAGA TGTTTGGAAA GGTTGCTATG AGAGATAACA ACCAGATCAA CAGGAACAAC 
AATTTCCAGA CCTTTCCTCA GGCGGTGCTG CTACTCTTCA GGTGTGCCAC GGGTGAAGCC 
TGGCAGGAGA TCATGCTGGC CTGTCTCCCG GGGAAGCTCT GTGACCCCGA GTCAGACTAT 
AACCCTGGGG AGGAGTACAC ATGTGGGAGC AACTTTGCCA TTGTTTATTT CATCAGTTTT 
TACATGCTCT GCGCATTTCT GATCATCAAC CTGTTTGTGG CTGTCATCAT GGACAACTTT 
GATTATCTGA CTCGGGACTG GTCCATTCTA GGGCCTCACC ATTTAGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAGGCAAAA GGAAGGATCA AGCACCTTGA TGTGGTCACT 
CTGCTCCGAC GCATCCAGCC TCCCCTGGGG TTTGGGAAAT TATGCCCACA CAGAGTAGCC 
TGTAAGAGAC TAGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTTAAT 
GCAACCCTGT TTGCTTTGGT TCGAACGGCT CTTAAAATCA AGACAGAAGG GAACCTGGAA 
CAAGCTAATG AGGAACTCCG AGCTGTGATA AAGAAAATCT GGAAGAAAAC CAGCATGAAA 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA ACAAGGACTG 
GTGGGAAAAT ACCCTGCGAA GAACACCACC ATTGCCCTAC AGGCAGGATT AAGGACACTG 
CATGACATTG GGCCAGAAAT CCGGCGTGCT ATATCATGTG ATTTGCAAGA TGACGAGCCG 
GAGGAAACAA AACGAGAAGA AGAAGAAGAT GGATTCAAAA GAAATGGTGC CCTGCTTGGA 
AACCATGTCA ATCATGTTAA TAGTGATAGG AGAGACCCCC TTCAGCAGAC CAATACCACC 
CACCGTCCCC TGCATGTCCA AAGGCCTTCA GTTCCACCTG CAAGTGATAC TGAGAGACCG 
CTGTTTCCTC CAGCAGGAAA TTCGGGGTGT CATAGCCATC ATAACCATAA TTCCGTAGGA 
AAGCAAGCTC CCAGCTCAAC AAATGCCAAT CTCAATAATG CCAATATGTC CAAAGCTGCC 
CCTGGAAAGC GGCCCAGCAT TGGGAACCTT GAGCATATGT CTGAAAATGG GCATCACTCC 
TCCCACAAGC ATGACCGGGA GCCTCAAAGA AGGTCCAGTA TTAAAAGAAC CCGCTATTAT 
GAAACTTACA TTAGATCTGA CTCGGGAGAT GAGCAGTTCC CAACCATCTG CCGAGAGGAT 
CCGGAGCCCC ACGGCTACTT CAGGGACGCC CGCTGCTTGG AGGAGCAGGA GTACTTCAGC 
GGCGAGGAGG GCTATGAGGA TGTGGGCTCT CCGGCCGGGG GCAGGCAGAG CTACAACTAC 
TACAACCGAT ACCCGGGCAG CAGCTGGGAC TTCGAGCGGC CCCGAGGCTA CCATCACCCC 
CAAGGCTTCT TGGAGGATGA CGACTCGCCC GTTTGCTATG ACTCCCGGAG ATCCCCAAGG 
AGACGCCTAC TACCTCCCAC CCCGACATCG CACCGGAGAT CCTCCTTCAA CTTTGAGTGC 
CTGCGCCGGC AGAGCAGCCA GGAGGAGGTC TCGCTGTCCC CCACCTTCCC CCACCGCACA 
GCCCTGCCAC TGCACCTGAT GCAGCAACAG ATCATGGCAG TTGCGGGCCT GGATTCCAGT 
AAAGCCCAGA AGTACTCGCC GAGCCACTCA ACCCGGTCGT GGGCCACCCC TCCAGCGACC 
CCTCCGTACC GAGACTGGAC GCCGTGCTAC GCGCCCCTGA TCCAGGTGGA GCGGCCAGAG 
GCCCTGGACC AGGTCAACGG CAGCCTTCCA TCCCTGCACC GAAGCTCATG GTACACGGAC 
GAGCCCGGCA TCTCATACCG AACTTTCACA CCAGCCAGCC TGACTGTCCC CAGCAGCTTC 
CGCAACAAAA ACAGTGACAA GCAGAGGAGT GCAGACAGCT TGGTGGAGGC AGTCCTGATA 
TCCGAAGGCT TAGGACGGTA TGCAAGGGAC CCAAAATTTG TATCGGCAAC AAAACACGAA 
ATCGCCGATG CCTGTGACCT GACCATCGAT GAGATGGAGA GCGCAGCCAG CAACCTGCTC 
AATGGGACTG TGCATCCCCG GGCTAACGGG GGCGTGGGCC CTGTCTCACA CCGGCAGGAC 
TATGAGCTCC AGGACTTTGG GCCGGGCTAC AGCGATGAGG AGCCTGACCT CGGAAGAGAT 
GAGGAGGACC TGGCAGATGA AATGATATGC ATCACCACCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262301.1
CDS	308..6832
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_022406593.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406593.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATAACTCT 
GAAGAGAGCA ATAGAATCTC CATCACCTTT TTCCGTCTTT TCCGAGTGAT GCGGTTGGTG 
AAACTTCTCA GCAGGGGGGA AGGCATCCGG ACGTTGCTGT GGACTTTTAT AAAGTCCTTT 
CAGGCTCTGC CCTATGTCGC CCTCCTCATA GCCATGCTGT TCTTCATCTA CGCGGTTATC 
GGCATGCAGA TGTTTGGAAA GGTTGCTATG AGAGATAACA ACCAGATCAA CAGGAACAAC 
AATTTCCAGA CCTTTCCTCA GGCGGTGCTG CTACTCTTCA GGTGTGCCAC GGGTGAAGCC 
TGGCAGGAGA TCATGCTGGC CTGTCTCCCG GGGAAGCTCT GTGACCCCGA GTCAGACTAT 
AACCCTGGGG AGGAGTACAC ATGTGGGAGC AACTTTGCCA TTGTTTATTT CATCAGTTTT 
TACATGCTCT GCGCATTTCT GATCATCAAC CTGTTTGTGG CTGTCATCAT GGACAACTTT 
GATTATCTGA CTCGGGACTG GTCCATTCTA GGGCCTCACC ATTTAGACGA ATTCAAAAGA 
ATATGGTCAG AATATGACCC TGAGGCAAAA GGAAGGATCA AGCACCTTGA TGTGGTCACT 
CTGCTCCGAC GCATCCAGCC TCCCCTGGGG TTTGGGAAAT TATGCCCACA CAGAGTAGCC 
TGTAAGAGAC TAGTTGCCAT GAACATGCCT CTCAACAGTG ATGGGACAGT CATGTTTAAT 
GCAACCCTGT TTGCTTTGGT TCGAACGGCT CTTAAAATCA AGACAGAAGG GAACCTGGAA 
CAAGCTAATG AGGAACTCCG AGCTGTGATA AAGAAAATCT GGAAGAAAAC CAGCATGAAA 
CTGCTTGACC AAGTTGTCCC TCCAGCTGGT GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACTTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGGAAAGA ACAAGGACTG 
GTGGGAAAAT ACCCTGCGAA GAACACCACC ATTGCCCTAC AGGCAGGATT AAGGACACTG 
CATGACATTG GGCCAGAAAT CCGGCGTGCT ATATCATGTG ATTTGCAAGA TGACGAGCCG 
GAGGAAACAA AACGAGAAGA AGAAGAAGAT GGATTCAAAA GAAATGGTGC CCTGCTTGGA 
AACCATGTCA ATCATGTTAA TAGTGATAGG AGAGACCCCC TTCAGCAGAC CAATACCACC 
CACCGTCCCC TGCATGTCCA AAGGCCTTCA GTTCCACCTG CAAGTGATAC TGAGAGACCG 
CTGTTTCCTC CAGCAGGAAA TTCGGGGTGT CATAGCCATC ATAACCATAA TTCCGTAGGA 
AAGCAAGCTC CCAGCTCAAC AAATGCCAAT CTCAATAATG CCAATATGTC CAAAGCTGCC 
CCTGGAAAGC GGCCCAGCAT TGGGAACCTT GAGCATATGT CTGAAAATGG GCATCACTCC 
TCCCACAAGC ATGACCGGGA GCCTCAAAGA AGGTCCAGTA TTAAAAGAAC CCGCTATTAT 
GAAACTTACA TTAGATCTGA CTCGGGAGAT GAGCAGTTCC CAACCATCTG CCGAGAGGAT 
CCGGAGCCCC ACGGCTACTT CAGGGACGCC CGCTGCTTGG AGGAGCAGGA GTACTTCAGC 
GGCGAGGAGG GCTATGAGGA TGTGGGCTCT CCGGCCGGGG GCAGGCAGAG CTACAACTAC 
TACAACCGAT ACCCGGGCAG CAGCTGGGAC TTCGAGCGGC CCCGAGGCTA CCATCACCCC 
CAAGGCTTCT TGGAGGATGA CGACTCGCCC GTTTGCTATG ACTCCCGGAG ATCCCCAAGG 
AGACGCCTAC TACCTCCCAC CCCGACATCG CACCGGAGAT CCTCCTTCAA CTTTGAGTGC 
CTGCGCCGGC AGAGCAGCCA GGAGGAGGTC TCGCTGTCCC CCACCTTCCC CCACCGCACA 
GCCCTGCCAC TGCACCTGAT GCAGCAACAG ATCATGGCAG TTGCGGGCCT GGATTCCAGT 
AAAGCCCAGA AGTACTCGCC GAGCCACTCA ACCCGGTCGT GGGCCACCCC TCCAGCGACC 
CCTCCGTACC GAGACTGGAC GCCGTGCTAC GCGCCCCTGA TCCAGGTGGA GCGGCCAGAG 
GCCCTGGACC AGGTCAACGG CAGCCTTCCA TCCCTGCACC GAAGCTCATG GTACACGGAC 
GAGCCCGGCA TCTCATACCG AACTTTCACA CCAGCCAGCC TGACTGTCCC CAGCAGCTTC 
CGCAACAAAA ACAGTGACAA GCAGAGGAGT GCAGACAGCT TGGTGGAGGC AGTCCTGATA 
TCCGAAGGCT TAGGACGGTA TGCAAGGGAC CCAAAATTTG TATCGGCAAC AAAACACGAA 
ATCGCCGATG CCTGTGACCT GACCATCGAT GAGATGGAGA GCGCAGCCAG CAACCTGCTC 
AATGGGACTG TGCATCCCCG GGCTAACGGG GGCGTGGGCC CTGTCTCACA CCGGCAGGAC 
TATGAGCTCC AGGACTTTGG GCCGGGCTAC AGCGATGAGG AGCCTGACCT CGGAAGAGAT 
GAGGAGGACC TGGCAGATGA AATGATATGC ATCACCACCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_022406594.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262302.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X20, mRNA.

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CloneID	OCe295377
Clone ID Related Accession (Same CDS sequence)	XM_022406594.1
Accession Version	XM_022406594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262302.1
CDS	308..6748
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_022406594.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406594.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_022406595.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262303.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X21, mRNA.

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CloneID	OCe295378
Clone ID Related Accession (Same CDS sequence)	XM_022406595.1
Accession Version	XM_022406595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262303.1
CDS	308..6748
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_022406595.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406595.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122083.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977558.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.

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CloneID	OCe41786
Clone ID Related Accession (Same CDS sequence)	XM_014122083.2 , XM_014122083.1
Accession Version	XM_014122083.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977558.1
CDS	308..6937
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122083.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122083.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122083.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977558.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X1, mRNA.

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CloneID	OCe41786
Clone ID Related Accession (Same CDS sequence)	XM_014122083.2 , XM_014122083.1
Accession Version	XM_014122083.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977558.1
CDS	296..6925
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122083.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122083.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122084.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977559.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.

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CloneID	OCe41787
Clone ID Related Accession (Same CDS sequence)	XM_014122084.2 , XM_014122084.1
Accession Version	XM_014122084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977559.1
CDS	308..6937
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122084.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122084.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122084.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977559.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X2, mRNA.

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CloneID	OCe41787
Clone ID Related Accession (Same CDS sequence)	XM_014122084.2 , XM_014122084.1
Accession Version	XM_014122084.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977559.1
CDS	296..6925
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122084.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122084.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122085.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977560.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.

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CloneID	OCe41788
Clone ID Related Accession (Same CDS sequence)	XM_014122085.2 , XM_014122085.1
Accession Version	XM_014122085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGATCTGACT CGGGAGATGA GCAGTTCCCA 
ACCATCTGCC GAGAGGATCC GGAGCCCCAC GGCTACTTCA GGGACGCCCG CTGCTTGGAG 
GAGCAGGAGT ACTTCAGCGG CGAGGAGGGC TATGAGGATG TGGGCTCTCC GGCCGGGGGC 
AGGCAGAGCT ACAACTACTA CAACCGATAC CCGGGCAGCA GCTGGGACTT CGAGCGGCCC 
CGAGGCTACC ATCACCCCCA AGGCTTCTTG GAGGATGACG ACTCGCCCGT TTGCTATGAC 
TCCCGGAGAT CCCCAAGGAG ACGCCTACTA CCTCCCACCC CGACATCGCA CCGGAGATCC 
TCCTTCAACT TTGAGTGCCT GCGCCGGCAG AGCAGCCAGG AGGAGGTCTC GCTGTCCCCC 
ACCTTCCCCC ACCGCACAGC CCTGCCACTG CACCTGATGC AGCAACAGAT CATGGCAGTT 
GCGGGCCTGG ATTCCAGTAA AGCCCAGAAG TACTCGCCGA GCCACTCAAC CCGGTCGTGG 
GCCACCCCTC CAGCGACCCC TCCGTACCGA GACTGGACGC CGTGCTACGC GCCCCTGATC 
CAGGTGGAGC GGCCAGAGGC CCTGGACCAG GTCAACGGCA GCCTTCCATC CCTGCACCGA 
AGCTCATGGT ACACGGACGA GCCCGGCATC TCATACCGAA CTTTCACACC AGCCAGCCTG 
ACTGTCCCCA GCAGCTTCCG CAACAAAAAC AGTGACAAGC AGAGGAGTGC AGACAGCTTG 
GTGGAGGCAG TCCTGATATC CGAAGGCTTA GGACGGTATG CAAGGGACCC AAAATTTGTA 
TCGGCAACAA AACACGAAAT CGCCGATGCC TGTGACCTGA CCATCGATGA GATGGAGAGC 
GCAGCCAGCA ACCTGCTCAA TGGGACTGTG CATCCCCGGG CTAACGGGGG CGTGGGCCCT 
GTCTCACACC GGCAGGACTA TGAGCTCCAG GACTTTGGGC CGGGCTACAG CGATGAGGAG 
CCTGACCTCG GAAGAGATGA GGAGGACCTG GCAGATGAAA TGATATGCAT CACCACCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977560.1
CDS	308..6910
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122085.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122085.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGATCTGACT CGGGAGATGA GCAGTTCCCA 
ACCATCTGCC GAGAGGATCC GGAGCCCCAC GGCTACTTCA GGGACGCCCG CTGCTTGGAG 
GAGCAGGAGT ACTTCAGCGG CGAGGAGGGC TATGAGGATG TGGGCTCTCC GGCCGGGGGC 
AGGCAGAGCT ACAACTACTA CAACCGATAC CCGGGCAGCA GCTGGGACTT CGAGCGGCCC 
CGAGGCTACC ATCACCCCCA AGGCTTCTTG GAGGATGACG ACTCGCCCGT TTGCTATGAC 
TCCCGGAGAT CCCCAAGGAG ACGCCTACTA CCTCCCACCC CGACATCGCA CCGGAGATCC 
TCCTTCAACT TTGAGTGCCT GCGCCGGCAG AGCAGCCAGG AGGAGGTCTC GCTGTCCCCC 
ACCTTCCCCC ACCGCACAGC CCTGCCACTG CACCTGATGC AGCAACAGAT CATGGCAGTT 
GCGGGCCTGG ATTCCAGTAA AGCCCAGAAG TACTCGCCGA GCCACTCAAC CCGGTCGTGG 
GCCACCCCTC CAGCGACCCC TCCGTACCGA GACTGGACGC CGTGCTACGC GCCCCTGATC 
CAGGTGGAGC GGCCAGAGGC CCTGGACCAG GTCAACGGCA GCCTTCCATC CCTGCACCGA 
AGCTCATGGT ACACGGACGA GCCCGGCATC TCATACCGAA CTTTCACACC AGCCAGCCTG 
ACTGTCCCCA GCAGCTTCCG CAACAAAAAC AGTGACAAGC AGAGGAGTGC AGACAGCTTG 
GTGGAGGCAG TCCTGATATC CGAAGGCTTA GGACGGTATG CAAGGGACCC AAAATTTGTA 
TCGGCAACAA AACACGAAAT CGCCGATGCC TGTGACCTGA CCATCGATGA GATGGAGAGC 
GCAGCCAGCA ACCTGCTCAA TGGGACTGTG CATCCCCGGG CTAACGGGGG CGTGGGCCCT 
GTCTCACACC GGCAGGACTA TGAGCTCCAG GACTTTGGGC CGGGCTACAG CGATGAGGAG 
CCTGACCTCG GAAGAGATGA GGAGGACCTG GCAGATGAAA TGATATGCAT CACCACCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122085.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977560.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X3, mRNA.

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CloneID	OCe41788
Clone ID Related Accession (Same CDS sequence)	XM_014122085.2 , XM_014122085.1
Accession Version	XM_014122085.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGATCTGACT CGGGAGATGA GCAGTTCCCA 
ACCATCTGCC GAGAGGATCC GGAGCCCCAC GGCTACTTCA GGGACGCCCG CTGCTTGGAG 
GAGCAGGAGT ACTTCAGCGG CGAGGAGGGC TATGAGGATG TGGGCTCTCC GGCCGGGGGC 
AGGCAGAGCT ACAACTACTA CAACCGATAC CCGGGCAGCA GCTGGGACTT CGAGCGGCCC 
CGAGGCTACC ATCACCCCCA AGGCTTCTTG GAGGATGACG ACTCGCCCGT TTGCTATGAC 
TCCCGGAGAT CCCCAAGGAG ACGCCTACTA CCTCCCACCC CGACATCGCA CCGGAGATCC 
TCCTTCAACT TTGAGTGCCT GCGCCGGCAG AGCAGCCAGG AGGAGGTCTC GCTGTCCCCC 
ACCTTCCCCC ACCGCACAGC CCTGCCACTG CACCTGATGC AGCAACAGAT CATGGCAGTT 
GCGGGCCTGG ATTCCAGTAA AGCCCAGAAG TACTCGCCGA GCCACTCAAC CCGGTCGTGG 
GCCACCCCTC CAGCGACCCC TCCGTACCGA GACTGGACGC CGTGCTACGC GCCCCTGATC 
CAGGTGGAGC GGCCAGAGGC CCTGGACCAG GTCAACGGCA GCCTTCCATC CCTGCACCGA 
AGCTCATGGT ACACGGACGA GCCCGGCATC TCATACCGAA CTTTCACACC AGCCAGCCTG 
ACTGTCCCCA GCAGCTTCCG CAACAAAAAC AGTGACAAGC AGAGGAGTGC AGACAGCTTG 
GTGGAGGCAG TCCTGATATC CGAAGGCTTA GGACGGTATG CAAGGGACCC AAAATTTGTA 
TCGGCAACAA AACACGAAAT CGCCGATGCC TGTGACCTGA CCATCGATGA GATGGAGAGC 
GCAGCCAGCA ACCTGCTCAA TGGGACTGTG CATCCCCGGG CTAACGGGGG CGTGGGCCCT 
GTCTCACACC GGCAGGACTA TGAGCTCCAG GACTTTGGGC CGGGCTACAG CGATGAGGAG 
CCTGACCTCG GAAGAGATGA GGAGGACCTG GCAGATGAAA TGATATGCAT CACCACCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977560.1
CDS	296..6898
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122085.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122085.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGATCTGACT CGGGAGATGA GCAGTTCCCA 
ACCATCTGCC GAGAGGATCC GGAGCCCCAC GGCTACTTCA GGGACGCCCG CTGCTTGGAG 
GAGCAGGAGT ACTTCAGCGG CGAGGAGGGC TATGAGGATG TGGGCTCTCC GGCCGGGGGC 
AGGCAGAGCT ACAACTACTA CAACCGATAC CCGGGCAGCA GCTGGGACTT CGAGCGGCCC 
CGAGGCTACC ATCACCCCCA AGGCTTCTTG GAGGATGACG ACTCGCCCGT TTGCTATGAC 
TCCCGGAGAT CCCCAAGGAG ACGCCTACTA CCTCCCACCC CGACATCGCA CCGGAGATCC 
TCCTTCAACT TTGAGTGCCT GCGCCGGCAG AGCAGCCAGG AGGAGGTCTC GCTGTCCCCC 
ACCTTCCCCC ACCGCACAGC CCTGCCACTG CACCTGATGC AGCAACAGAT CATGGCAGTT 
GCGGGCCTGG ATTCCAGTAA AGCCCAGAAG TACTCGCCGA GCCACTCAAC CCGGTCGTGG 
GCCACCCCTC CAGCGACCCC TCCGTACCGA GACTGGACGC CGTGCTACGC GCCCCTGATC 
CAGGTGGAGC GGCCAGAGGC CCTGGACCAG GTCAACGGCA GCCTTCCATC CCTGCACCGA 
AGCTCATGGT ACACGGACGA GCCCGGCATC TCATACCGAA CTTTCACACC AGCCAGCCTG 
ACTGTCCCCA GCAGCTTCCG CAACAAAAAC AGTGACAAGC AGAGGAGTGC AGACAGCTTG 
GTGGAGGCAG TCCTGATATC CGAAGGCTTA GGACGGTATG CAAGGGACCC AAAATTTGTA 
TCGGCAACAA AACACGAAAT CGCCGATGCC TGTGACCTGA CCATCGATGA GATGGAGAGC 
GCAGCCAGCA ACCTGCTCAA TGGGACTGTG CATCCCCGGG CTAACGGGGG CGTGGGCCCT 
GTCTCACACC GGCAGGACTA TGAGCTCCAG GACTTTGGGC CGGGCTACAG CGATGAGGAG 
CCTGACCTCG GAAGAGATGA GGAGGACCTG GCAGATGAAA TGATATGCAT CACCACCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122086.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977561.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X4, mRNA.

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CloneID	OCe41789
Clone ID Related Accession (Same CDS sequence)	XM_014122086.1 , XM_014122086.2
Accession Version	XM_014122086.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977561.1
CDS	296..6865
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122086.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122086.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122086.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977561.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.

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CloneID	OCe41789
Clone ID Related Accession (Same CDS sequence)	XM_014122086.1 , XM_014122086.2
Accession Version	XM_014122086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977561.1
CDS	308..6877
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122086.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122086.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122087.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977562.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.

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CloneID	OCe41790
Clone ID Related Accession (Same CDS sequence)	XM_014122087.2 , XM_014122087.1
Accession Version	XM_014122087.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977562.1
CDS	308..6877
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122087.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122087.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122087.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977562.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X5, mRNA.

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CloneID	OCe41790
Clone ID Related Accession (Same CDS sequence)	XM_014122087.2 , XM_014122087.1
Accession Version	XM_014122087.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977562.1
CDS	296..6865
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122087.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122087.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGGCA GGATTAAGGA CACTGCATGA CATTGGGCCA 
GAAATCCGGC GTGCTATATC ATGTGATTTG CAAGATGACG AGCCGGAGGA AACAAAACGA 
GAAGAAGAAG AAGATGGATT CAAAAGAAAT GGTGCCCTGC TTGGAAACCA TGTCAATCAT 
GTTAATAGTG ATAGGAGAGA CCCCCTTCAG CAGACCAATA CCACCCACCG TCCCCTGCAT 
GTCCAAAGGC CTTCAGTTCC ACCTGCAAGT GATACTGAGA GACCGCTGTT TCCTCCAGCA 
GGAAATTCGG GGTGTCATAG CCATCATAAC CATAATTCCG TAGGAAAGCA AGCTCCCAGC 
TCAACAAATG CCAATCTCAA TAATGCCAAT ATGTCCAAAG CTGCCCCTGG AAAGCGGCCC 
AGCATTGGGA ACCTTGAGCA TATGTCTGAA AATGGGCATC ACTCCTCCCA CAAGCATGAC 
CGGGAGCCTC AAAGAAGGTC CAGTATTAAA AGAACCCGCT ATTATGAAAC TTACATTAGA 
TCTGACTCGG GAGATGAGCA GTTCCCAACC ATCTGCCGAG AGGATCCGGA GCCCCACGGC 
TACTTCAGGG ACGCCCGCTG CTTGGAGGAG CAGGAGTACT TCAGCGGCGA GGAGGGCTAT 
GAGGATGTGG GCTCTCCGGC CGGGGGCAGG CAGAGCTACA ACTACTACAA CCGATACCCG 
GGCAGCAGCT GGGACTTCGA GCGGCCCCGA GGCTACCATC ACCCCCAAGG CTTCTTGGAG 
GATGACGACT CGCCCGTTTG CTATGACTCC CGGAGATCCC CAAGGAGACG CCTACTACCT 
CCCACCCCGA CATCGCACCG GAGATCCTCC TTCAACTTTG AGTGCCTGCG CCGGCAGAGC 
AGCCAGGAGG AGGTCTCGCT GTCCCCCACC TTCCCCCACC GCACAGCCCT GCCACTGCAC 
CTGATGCAGC AACAGATCAT GGCAGTTGCG GGCCTGGATT CCAGTAAAGC CCAGAAGTAC 
TCGCCGAGCC ACTCAACCCG GTCGTGGGCC ACCCCTCCAG CGACCCCTCC GTACCGAGAC 
TGGACGCCGT GCTACGCGCC CCTGATCCAG GTGGAGCGGC CAGAGGCCCT GGACCAGGTC 
AACGGCAGCC TTCCATCCCT GCACCGAAGC TCATGGTACA CGGACGAGCC CGGCATCTCA 
TACCGAACTT TCACACCAGC CAGCCTGACT GTCCCCAGCA GCTTCCGCAA CAAAAACAGT 
GACAAGCAGA GGAGTGCAGA CAGCTTGGTG GAGGCAGTCC TGATATCCGA AGGCTTAGGA 
CGGTATGCAA GGGACCCAAA ATTTGTATCG GCAACAAAAC ACGAAATCGC CGATGCCTGT 
GACCTGACCA TCGATGAGAT GGAGAGCGCA GCCAGCAACC TGCTCAATGG GACTGTGCAT 
CCCCGGGCTA ACGGGGGCGT GGGCCCTGTC TCACACCGGC AGGACTATGA GCTCCAGGAC 
TTTGGGCCGG GCTACAGCGA TGAGGAGCCT GACCTCGGAA GAGATGAGGA GGACCTGGCA 
GATGAAATGA TATGCATCAC CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122088.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977563.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X7, mRNA.

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CloneID	OCe41791
Clone ID Related Accession (Same CDS sequence)	XM_014122088.1 , XM_014122088.2
Accession Version	XM_014122088.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977563.1
CDS	296..6841
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122088.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122088.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122088.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977563.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.

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CloneID	OCe41791
Clone ID Related Accession (Same CDS sequence)	XM_014122088.1 , XM_014122088.2
Accession Version	XM_014122088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977563.1
CDS	308..6853
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122088.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122088.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122089.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977564.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X8, mRNA.

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CloneID	OCe41792
Clone ID Related Accession (Same CDS sequence)	XM_014122089.1 , XM_014122089.2
Accession Version	XM_014122089.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGAGA AATGGTGCCC TGCTTGGAAA CCATGTCAAT 
CATGTTAATA GTGATAGGAG AGACCCCCTT CAGCAGACCA ATACCACCCA CCGTCCCCTG 
CATGTCCAAA GGCCTTCAGT TCCACCTGCA AGTGATACTG AGAGACCGCT GTTTCCTCCA 
GCAGGAAATT CGGGGTGTCA TAGCCATCAT AACCATAATT CCGTAGGAAA GCAAGCTCCC 
AGCTCAACAA ATGCCAATCT CAATAATGCC AATATGTCCA AAGCTGCCCC TGGAAAGCGG 
CCCAGCATTG GGAACCTTGA GCATATGTCT GAAAATGGGC ATCACTCCTC CCACAAGCAT 
GACCGGGAGC CTCAAAGAAG GTCCAGTATT AAAAGAACCC GCTATTATGA AACTTACATT 
AGATCTGACT CGGGAGATGA GCAGTTCCCA ACCATCTGCC GAGAGGATCC GGAGCCCCAC 
GGCTACTTCA GGGACGCCCG CTGCTTGGAG GAGCAGGAGT ACTTCAGCGG CGAGGAGGGC 
TATGAGGATG TGGGCTCTCC GGCCGGGGGC AGGCAGAGCT ACAACTACTA CAACCGATAC 
CCGGGCAGCA GCTGGGACTT CGAGCGGCCC CGAGGCTACC ATCACCCCCA AGGCTTCTTG 
GAGGATGACG ACTCGCCCGT TTGCTATGAC TCCCGGAGAT CCCCAAGGAG ACGCCTACTA 
CCTCCCACCC CGACATCGCA CCGGAGATCC TCCTTCAACT TTGAGTGCCT GCGCCGGCAG 
AGCAGCCAGG AGGAGGTCTC GCTGTCCCCC ACCTTCCCCC ACCGCACAGC CCTGCCACTG 
CACCTGATGC AGCAACAGAT CATGGCAGTT GCGGGCCTGG ATTCCAGTAA AGCCCAGAAG 
TACTCGCCGA GCCACTCAAC CCGGTCGTGG GCCACCCCTC CAGCGACCCC TCCGTACCGA 
GACTGGACGC CGTGCTACGC GCCCCTGATC CAGGTGGAGC GGCCAGAGGC CCTGGACCAG 
GTCAACGGCA GCCTTCCATC CCTGCACCGA AGCTCATGGT ACACGGACGA GCCCGGCATC 
TCATACCGAA CTTTCACACC AGCCAGCCTG ACTGTCCCCA GCAGCTTCCG CAACAAAAAC 
AGTGACAAGC AGAGGAGTGC AGACAGCTTG GTGGAGGCAG TCCTGATATC CGAAGGCTTA 
GGACGGTATG CAAGGGACCC AAAATTTGTA TCGGCAACAA AACACGAAAT CGCCGATGCC 
TGTGACCTGA CCATCGATGA GATGGAGAGC GCAGCCAGCA ACCTGCTCAA TGGGACTGTG 
CATCCCCGGG CTAACGGGGG CGTGGGCCCT GTCTCACACC GGCAGGACTA TGAGCTCCAG 
GACTTTGGGC CGGGCTACAG CGATGAGGAG CCTGACCTCG GAAGAGATGA GGAGGACCTG 
GCAGATGAAA TGATATGCAT CACCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977564.1
CDS	296..6808
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122089.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122089.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGAGA AATGGTGCCC TGCTTGGAAA CCATGTCAAT 
CATGTTAATA GTGATAGGAG AGACCCCCTT CAGCAGACCA ATACCACCCA CCGTCCCCTG 
CATGTCCAAA GGCCTTCAGT TCCACCTGCA AGTGATACTG AGAGACCGCT GTTTCCTCCA 
GCAGGAAATT CGGGGTGTCA TAGCCATCAT AACCATAATT CCGTAGGAAA GCAAGCTCCC 
AGCTCAACAA ATGCCAATCT CAATAATGCC AATATGTCCA AAGCTGCCCC TGGAAAGCGG 
CCCAGCATTG GGAACCTTGA GCATATGTCT GAAAATGGGC ATCACTCCTC CCACAAGCAT 
GACCGGGAGC CTCAAAGAAG GTCCAGTATT AAAAGAACCC GCTATTATGA AACTTACATT 
AGATCTGACT CGGGAGATGA GCAGTTCCCA ACCATCTGCC GAGAGGATCC GGAGCCCCAC 
GGCTACTTCA GGGACGCCCG CTGCTTGGAG GAGCAGGAGT ACTTCAGCGG CGAGGAGGGC 
TATGAGGATG TGGGCTCTCC GGCCGGGGGC AGGCAGAGCT ACAACTACTA CAACCGATAC 
CCGGGCAGCA GCTGGGACTT CGAGCGGCCC CGAGGCTACC ATCACCCCCA AGGCTTCTTG 
GAGGATGACG ACTCGCCCGT TTGCTATGAC TCCCGGAGAT CCCCAAGGAG ACGCCTACTA 
CCTCCCACCC CGACATCGCA CCGGAGATCC TCCTTCAACT TTGAGTGCCT GCGCCGGCAG 
AGCAGCCAGG AGGAGGTCTC GCTGTCCCCC ACCTTCCCCC ACCGCACAGC CCTGCCACTG 
CACCTGATGC AGCAACAGAT CATGGCAGTT GCGGGCCTGG ATTCCAGTAA AGCCCAGAAG 
TACTCGCCGA GCCACTCAAC CCGGTCGTGG GCCACCCCTC CAGCGACCCC TCCGTACCGA 
GACTGGACGC CGTGCTACGC GCCCCTGATC CAGGTGGAGC GGCCAGAGGC CCTGGACCAG 
GTCAACGGCA GCCTTCCATC CCTGCACCGA AGCTCATGGT ACACGGACGA GCCCGGCATC 
TCATACCGAA CTTTCACACC AGCCAGCCTG ACTGTCCCCA GCAGCTTCCG CAACAAAAAC 
AGTGACAAGC AGAGGAGTGC AGACAGCTTG GTGGAGGCAG TCCTGATATC CGAAGGCTTA 
GGACGGTATG CAAGGGACCC AAAATTTGTA TCGGCAACAA AACACGAAAT CGCCGATGCC 
TGTGACCTGA CCATCGATGA GATGGAGAGC GCAGCCAGCA ACCTGCTCAA TGGGACTGTG 
CATCCCCGGG CTAACGGGGG CGTGGGCCCT GTCTCACACC GGCAGGACTA TGAGCTCCAG 
GACTTTGGGC CGGGCTACAG CGATGAGGAG CCTGACCTCG GAAGAGATGA GGAGGACCTG 
GCAGATGAAA TGATATGCAT CACCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122089.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977564.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.

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CloneID	OCe41792
Clone ID Related Accession (Same CDS sequence)	XM_014122089.1 , XM_014122089.2
Accession Version	XM_014122089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGAGA AATGGTGCCC TGCTTGGAAA CCATGTCAAT 
CATGTTAATA GTGATAGGAG AGACCCCCTT CAGCAGACCA ATACCACCCA CCGTCCCCTG 
CATGTCCAAA GGCCTTCAGT TCCACCTGCA AGTGATACTG AGAGACCGCT GTTTCCTCCA 
GCAGGAAATT CGGGGTGTCA TAGCCATCAT AACCATAATT CCGTAGGAAA GCAAGCTCCC 
AGCTCAACAA ATGCCAATCT CAATAATGCC AATATGTCCA AAGCTGCCCC TGGAAAGCGG 
CCCAGCATTG GGAACCTTGA GCATATGTCT GAAAATGGGC ATCACTCCTC CCACAAGCAT 
GACCGGGAGC CTCAAAGAAG GTCCAGTATT AAAAGAACCC GCTATTATGA AACTTACATT 
AGATCTGACT CGGGAGATGA GCAGTTCCCA ACCATCTGCC GAGAGGATCC GGAGCCCCAC 
GGCTACTTCA GGGACGCCCG CTGCTTGGAG GAGCAGGAGT ACTTCAGCGG CGAGGAGGGC 
TATGAGGATG TGGGCTCTCC GGCCGGGGGC AGGCAGAGCT ACAACTACTA CAACCGATAC 
CCGGGCAGCA GCTGGGACTT CGAGCGGCCC CGAGGCTACC ATCACCCCCA AGGCTTCTTG 
GAGGATGACG ACTCGCCCGT TTGCTATGAC TCCCGGAGAT CCCCAAGGAG ACGCCTACTA 
CCTCCCACCC CGACATCGCA CCGGAGATCC TCCTTCAACT TTGAGTGCCT GCGCCGGCAG 
AGCAGCCAGG AGGAGGTCTC GCTGTCCCCC ACCTTCCCCC ACCGCACAGC CCTGCCACTG 
CACCTGATGC AGCAACAGAT CATGGCAGTT GCGGGCCTGG ATTCCAGTAA AGCCCAGAAG 
TACTCGCCGA GCCACTCAAC CCGGTCGTGG GCCACCCCTC CAGCGACCCC TCCGTACCGA 
GACTGGACGC CGTGCTACGC GCCCCTGATC CAGGTGGAGC GGCCAGAGGC CCTGGACCAG 
GTCAACGGCA GCCTTCCATC CCTGCACCGA AGCTCATGGT ACACGGACGA GCCCGGCATC 
TCATACCGAA CTTTCACACC AGCCAGCCTG ACTGTCCCCA GCAGCTTCCG CAACAAAAAC 
AGTGACAAGC AGAGGAGTGC AGACAGCTTG GTGGAGGCAG TCCTGATATC CGAAGGCTTA 
GGACGGTATG CAAGGGACCC AAAATTTGTA TCGGCAACAA AACACGAAAT CGCCGATGCC 
TGTGACCTGA CCATCGATGA GATGGAGAGC GCAGCCAGCA ACCTGCTCAA TGGGACTGTG 
CATCCCCGGG CTAACGGGGG CGTGGGCCCT GTCTCACACC GGCAGGACTA TGAGCTCCAG 
GACTTTGGGC CGGGCTACAG CGATGAGGAG CCTGACCTCG GAAGAGATGA GGAGGACCTG 
GCAGATGAAA TGATATGCAT CACCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977564.1
CDS	309..6821
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122089.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122089.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC CACTATTTCA CTGATGCATG GAACACTTTT 
GATGCCTTAA TTGTTGTTGG TAGCGTCGTT GATATTGCTA TAACTGAAGT GAATCCAACT 
GAAAGTGAAA ATGTCCCTGT CCCAACCGCC ACACCCGGGA ACTCTGAAGA GAGCAATAGA 
ATCTCCATCA CCTTTTTCCG TCTTTTCCGA GTGATGCGGT TGGTGAAACT TCTCAGCAGG 
GGGGAAGGCA TCCGGACGTT GCTGTGGACT TTTATAAAGT CCTTTCAGGC TCTGCCCTAT 
GTCGCCCTCC TCATAGCCAT GCTGTTCTTC ATCTACGCGG TTATCGGCAT GCAGATGTTT 
GGAAAGGTTG CTATGAGAGA TAACAACCAG ATCAACAGGA ACAACAATTT CCAGACCTTT 
CCTCAGGCGG TGCTGCTACT CTTCAGGTGT GCCACGGGTG AAGCCTGGCA GGAGATCATG 
CTGGCCTGTC TCCCGGGGAA GCTCTGTGAC CCCGAGTCAG ACTATAACCC TGGGGAGGAG 
TACACATGTG GGAGCAACTT TGCCATTGTT TATTTCATCA GTTTTTACAT GCTCTGCGCA 
TTTCTGATCA TCAACCTGTT TGTGGCTGTC ATCATGGACA ACTTTGATTA TCTGACTCGG 
GACTGGTCCA TTCTAGGGCC TCACCATTTA GACGAATTCA AAAGAATATG GTCAGAATAT 
GACCCTGAGG CAAAAGGAAG GATCAAGCAC CTTGATGTGG TCACTCTGCT CCGACGCATC 
CAGCCTCCCC TGGGGTTTGG GAAATTATGC CCACACAGAG TAGCCTGTAA GAGACTAGTT 
GCCATGAACA TGCCTCTCAA CAGTGATGGG ACAGTCATGT TTAATGCAAC CCTGTTTGCT 
TTGGTTCGAA CGGCTCTTAA AATCAAGACA GAAGGGAACC TGGAACAAGC TAATGAGGAA 
CTCCGAGCTG TGATAAAGAA AATCTGGAAG AAAACCAGCA TGAAACTGCT TGACCAAGTT 
GTCCCTCCAG CTGGTGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC TTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGG AAAGAACAAG GACTGGTGGG AAAATACCCT 
GCGAAGAACA CCACCATTGC CCTACAGAGA AATGGTGCCC TGCTTGGAAA CCATGTCAAT 
CATGTTAATA GTGATAGGAG AGACCCCCTT CAGCAGACCA ATACCACCCA CCGTCCCCTG 
CATGTCCAAA GGCCTTCAGT TCCACCTGCA AGTGATACTG AGAGACCGCT GTTTCCTCCA 
GCAGGAAATT CGGGGTGTCA TAGCCATCAT AACCATAATT CCGTAGGAAA GCAAGCTCCC 
AGCTCAACAA ATGCCAATCT CAATAATGCC AATATGTCCA AAGCTGCCCC TGGAAAGCGG 
CCCAGCATTG GGAACCTTGA GCATATGTCT GAAAATGGGC ATCACTCCTC CCACAAGCAT 
GACCGGGAGC CTCAAAGAAG GTCCAGTATT AAAAGAACCC GCTATTATGA AACTTACATT 
AGATCTGACT CGGGAGATGA GCAGTTCCCA ACCATCTGCC GAGAGGATCC GGAGCCCCAC 
GGCTACTTCA GGGACGCCCG CTGCTTGGAG GAGCAGGAGT ACTTCAGCGG CGAGGAGGGC 
TATGAGGATG TGGGCTCTCC GGCCGGGGGC AGGCAGAGCT ACAACTACTA CAACCGATAC 
CCGGGCAGCA GCTGGGACTT CGAGCGGCCC CGAGGCTACC ATCACCCCCA AGGCTTCTTG 
GAGGATGACG ACTCGCCCGT TTGCTATGAC TCCCGGAGAT CCCCAAGGAG ACGCCTACTA 
CCTCCCACCC CGACATCGCA CCGGAGATCC TCCTTCAACT TTGAGTGCCT GCGCCGGCAG 
AGCAGCCAGG AGGAGGTCTC GCTGTCCCCC ACCTTCCCCC ACCGCACAGC CCTGCCACTG 
CACCTGATGC AGCAACAGAT CATGGCAGTT GCGGGCCTGG ATTCCAGTAA AGCCCAGAAG 
TACTCGCCGA GCCACTCAAC CCGGTCGTGG GCCACCCCTC CAGCGACCCC TCCGTACCGA 
GACTGGACGC CGTGCTACGC GCCCCTGATC CAGGTGGAGC GGCCAGAGGC CCTGGACCAG 
GTCAACGGCA GCCTTCCATC CCTGCACCGA AGCTCATGGT ACACGGACGA GCCCGGCATC 
TCATACCGAA CTTTCACACC AGCCAGCCTG ACTGTCCCCA GCAGCTTCCG CAACAAAAAC 
AGTGACAAGC AGAGGAGTGC AGACAGCTTG GTGGAGGCAG TCCTGATATC CGAAGGCTTA 
GGACGGTATG CAAGGGACCC AAAATTTGTA TCGGCAACAA AACACGAAAT CGCCGATGCC 
TGTGACCTGA CCATCGATGA GATGGAGAGC GCAGCCAGCA ACCTGCTCAA TGGGACTGTG 
CATCCCCGGG CTAACGGGGG CGTGGGCCCT GTCTCACACC GGCAGGACTA TGAGCTCCAG 
GACTTTGGGC CGGGCTACAG CGATGAGGAG CCTGACCTCG GAAGAGATGA GGAGGACCTG 
GCAGATGAAA TGATATGCAT CACCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122090.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977565.1 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X10, mRNA.

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CloneID	OCe41793
Clone ID Related Accession (Same CDS sequence)	XM_014122090.2 , XM_014122090.1
Accession Version	XM_014122090.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977565.1
CDS	308..6808
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122090.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122090.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122090.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977565.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X9, mRNA.

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CloneID	OCe41793
Clone ID Related Accession (Same CDS sequence)	XM_014122090.2 , XM_014122090.1
Accession Version	XM_014122090.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977565.1
CDS	296..6796
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLWCWWKRRGTAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122090.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122090.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGGT GCTGGTGGAA AAGAAGAGGC 
ACGGCCAAGG CTGGGCCCTC TGGGTGTCGG CGGTGGGGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCCGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGG TTGGTGAAAC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACGT TGCTGTGGAC TTTTATAAAG TCCTTTCAGG CTCTGCCCTA TGTCGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTACGCG GTTATCGGCA TGCAGATGTT TGGAAAGGTT 
GCTATGAGAG ATAACAACCA GATCAACAGG AACAACAATT TCCAGACCTT TCCTCAGGCG 
GTGCTGCTAC TCTTCAGGTG TGCCACGGGT GAAGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCGGGGA AGCTCTGTGA CCCCGAGTCA GACTATAACC CTGGGGAGGA GTACACATGT 
GGGAGCAACT TTGCCATTGT TTATTTCATC AGTTTTTACA TGCTCTGCGC ATTTCTGATC 
ATCAACCTGT TTGTGGCTGT CATCATGGAC AACTTTGATT ATCTGACTCG GGACTGGTCC 
ATTCTAGGGC CTCACCATTT AGACGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAAGGAA GGATCAAGCA CCTTGATGTG GTCACTCTGC TCCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAATTATG CCCACACAGA GTAGCCTGTA AGAGACTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGATGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AAATCAAGAC AGAAGGGAAC CTGGAACAAG CTAATGAGGA ACTCCGAGCT 
GTGATAAAGA AAATCTGGAA GAAAACCAGC ATGAAACTGC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAATACCC TGCGAAGAAC 
ACCACCATTG CCCTACAGGC AGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CATGTGATTT GCAAGATGAC GAGCCGGAGG AAACAAAACG AGAAGAAGAA 
GAAGATGGAT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ACCCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAGTTC CACCTGCAAG TGATACTGAG AGACCGCTGT TTCCTCCAGC AGGAAATTCG 
GGGTGTCATA GCCATCATAA CCATAATTCC GTAGGAAAGC AAGCTCCCAG CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCCTG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATATGTCTGA AAATGGGCAT CACTCCTCCC ACAAGCATGA CCGGGAGCCT 
CAAAGAAGGT CCAGTATTAA AAGAACCCGC TATTATGAAA CTTACATTAG ATCTGACTCG 
GGAGATGAGC AGTTCCCAAC CATCTGCCGA GAGGATCCGG AGCCCCACGG CTACTTCAGG 
GACGCCCGCT GCTTGGAGGA GCAGGAGTAC TTCAGCGGCG AGGAGGGCTA TGAGGATGTG 
GGCTCTCCGG CCGGGGGCAG GCAGAGCTAC AACTACTACA ACCGATACCC GGGCAGCAGC 
TGGGACTTCG AGCGGCCCCG AGGCTACCAT CACCCCCAAG GCTTCTTGGA GGATGACGAC 
TCGCCCGTTT GCTATGACTC CCGGAGATCC CCAAGGAGAC GCCTACTACC TCCCACCCCG 
ACATCGCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAG 
GAGGTCTCGC TGTCCCCCAC CTTCCCCCAC CGCACAGCCC TGCCACTGCA CCTGATGCAG 
CAACAGATCA TGGCAGTTGC GGGCCTGGAT TCCAGTAAAG CCCAGAAGTA CTCGCCGAGC 
CACTCAACCC GGTCGTGGGC CACCCCTCCA GCGACCCCTC CGTACCGAGA CTGGACGCCG 
TGCTACGCGC CCCTGATCCA GGTGGAGCGG CCAGAGGCCC TGGACCAGGT CAACGGCAGC 
CTTCCATCCC TGCACCGAAG CTCATGGTAC ACGGACGAGC CCGGCATCTC ATACCGAACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGCA ACAAAAACAG TGACAAGCAG 
AGGAGTGCAG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTAGG ACGGTATGCA 
AGGGACCCAA AATTTGTATC GGCAACAAAA CACGAAATCG CCGATGCCTG TGACCTGACC 
ATCGATGAGA TGGAGAGCGC AGCCAGCAAC CTGCTCAATG GGACTGTGCA TCCCCGGGCT 
AACGGGGGCG TGGGCCCTGT CTCACACCGG CAGGACTATG AGCTCCAGGA CTTTGGGCCG 
GGCTACAGCG ATGAGGAGCC TGACCTCGGA AGAGATGAGG AGGACCTGGC AGATGAAATG 
ATATGCATCA CCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122093.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977568.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X15, mRNA.

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CloneID	OCe41796
Clone ID Related Accession (Same CDS sequence)	XM_014122093.1
Accession Version	XM_014122093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977568.1
CDS	296..6781
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122093.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122093.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_014122094.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977569.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X16, mRNA.

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CloneID	OCe41797
Clone ID Related Accession (Same CDS sequence)	XM_014122094.1
Accession Version	XM_014122094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977569.1
CDS	296..6781
Translation	MMMMMMMKNMHHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIIAEEDPAPCAFSGNGRQCAANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEESKRNTSMPTSETESVNTENVSGGGENPGGCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMATFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDNPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEEDGFKRNGALLGNHVNHVNSDRRDPLQQTNTTHRPLHVQRPSVPPASDTERPLFPPAGNSGCHSHHNHNSVGKQAPSSTNANLNNANMSKAAPGKRPSIGNLEHMSENGHHSSHKHDREPQRRSSIKRTRYYETYIRSDSGDEQFPTICREDPEPHGYFRDARCLEEQEYFSGEEGYEDVGSPAGGRQSYNYYNRYPGSSWDFERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPTSHRRSSFNFECLRRQSSQEEVSLSPTFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYAPLIQVERPEALDQVNGSLPSLHRSSWYTDEPGISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASNLLNGTVHPRANGGVGPVSHRQDYELQDFGPGYSDEEPDLGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_014122094.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122094.1

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAACATG CACCACCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTCCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACCGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT GGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTCTT CTGTTTGTCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATTGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTC TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GTAACCACTC CAGTGGCAAG TCAGGAGGCT TCGATGTCAA AGCCCTCCGC 
GCCTTTCGGG TGTTACGACC ACTTCGACTC GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTCCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACC 
TGTTTTTTTG CTGACTCAGA TATCATAGCT GAAGAGGACC CAGCGCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTGC CGCCAATGGC ACGGAGTGTA GGAGTGGCTG GGTTGGCCCA 
AATGGAGGCA TCACCAATTT TGATAATTTT GCCTTTGCCA TGCTCACCGT GTTCCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCTAA AGCCCGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCAGAAGA CATTGATCCT GAGAACGAGG AAGAAGGAGG AGAGGAAAGC 
AAACGAAACA CCAGCATGCC CACCAGCGAG ACCGAGTCGG TGAACACAGA GAACGTAAGT 
GGCGGAGGCG AGAATCCAGG CGGCTGTGGG AGTCTCTGCC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGC TTCAATCGCA GAAGATGTCG TGCTGCTGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATT GTCCTGGTGT TTCTCAATAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCTGACTGG TTGACGCAGA TTCAAGATAT CGCTAACAAA 
GTCCTCCTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGTCTCCAG 
GCCTATTTCG TCTCTCTCTT CAATCGGTTT GATTGCTTCG TGGTGTGTGG TGGCATTACC 
GAGACCATCC TGGTGGAGCT GGAAATCATG TCTCCTCTGG GGATCTCTGT GTTTCGGTGC 
GTGCGCCTCT TAAGGATTTT CAAGGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCCTCCTTGT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATCATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGTG GCAAGTTTAA TTTTGACGAA 
ACACAGACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CCCTTCTGAC CGTATTCCAG 
ATCCTGACAG GGGAGGACTG GAACGCCGTG ATGTATGATG GCATTATGGC CTATGGCGGC 
CCGTCATCTT CGGGGATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATCCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATCTCGCTGA TGCTGAAAGT 
TTGAATACTG CCCAGAAAGA GGAAGCTGAA GAGAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTGG AGAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AATAAGGTTA CAATTGATGA CTACCGAGAA GAGGATGAAG ACAAGGACCC CTACCCACCT 
TGTGATGTGC CAGTAGGGGA AGAGGAAGAG GAGGAGGAAG AGGATGAACC TGAGGTTCCT 
GCTGGCCCCC GTCCTCGCAG AATCTCAGAG TTGAACATGA AGGAGAAAAT CGCCCCCATC 
CCTGAAGGAA GTGCATTCTT CATTCTGAGC AAGACCAACC CGATCCGCGT GGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTCG TCTTCATCAT GCTGAGTAGT 
GCCGCCCTTG CCGCCGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC TATACTGGGT 
TACTTTGACT ATGCTTTCAC AGCCATCTTT ACTGTTGAAA TCCTGTTAAA GATGGCGACT 
TTTGGAGCTT TCCTCCACAA AGGGGCTTTC TGCAGAAACT ACTTCAATCT GCTGGATATG 
CTGGTTGTTG GGGTGTCACT GGTATCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGG CCACTCAGGG CAATAAACAG AGCAAAAGGA 
CTTAAGCATG TGGTCCAGTG TGTCTTCGTG GCCATCCGGA CCATTGGCAA CATCATGATC 
GTCACCACCC TTCTCCAGTT CATGTTTGCC TGCATCGGAG TACAGTTGTT CAAGGGAAAG 
TTCTATCGCT GTACAGATGA AGCCAAAAGT AACCCCGAAG AGTGCAGGGG GCTTTTCATC 
CTCTACAAGG ATGGGGATGT TGATAACCCT GTGGTCCGGG AGAGAATCTG GCAAAACAGC 
GATTTTAACT TTGACAACGT CCTTTCTGCT ATGATGGCGC TGTTCACGGT CTCCACGTTC 
GAGGGCTGGC CTGCGTTACT GTATAAAGCC ATCGACTCAA ATGGAGAGAA TGTCGGCCCC 
GTCTACAATT ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATCGTGGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATCGTCA CATTTCAGGA GCAGGGAGAG 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CCTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGACGCCAT GGACATTCTG 
AACATGGTCT TCACTGGGGT GTTCACTGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAA 
CCTAAGCACT ATTTCACTGA TGCATGGAAC ACTTTTGATG CCTTAATTGT TGTTGGTAGC 
GTCGTTGATA TTGCTATAAC TGAAGTGAAT CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACCGCCACAC CCGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGGTTGGT GAAACTTCTC AGCAGGGGGG AAGGCATCCG GACGTTGCTG 
TGGACTTTTA TAAAGTCCTT TCAGGCTCTG CCCTATGTCG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ACGCGGTTAT CGGCATGCAG ATGTTTGGAA AGGTTGCTAT GAGAGATAAC 
AACCAGATCA ACAGGAACAA CAATTTCCAG ACCTTTCCTC AGGCGGTGCT GCTACTCTTC 
AGGTGTGCCA CGGGTGAAGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC GGGGAAGCTC 
TGTGACCCCG AGTCAGACTA TAACCCTGGG GAGGAGTACA CATGTGGGAG CAACTTTGCC 
ATTGTTTATT TCATCAGTTT TTACATGCTC TGCGCATTTC TGATCATCAA CCTGTTTGTG 
GCTGTCATCA TGGACAACTT TGATTATCTG ACTCGGGACT GGTCCATTCT AGGGCCTCAC 
CATTTAGACG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA AGGAAGGATC 
AAGCACCTTG ATGTGGTCAC TCTGCTCCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAA 
TTATGCCCAC ACAGAGTAGC CTGTAAGAGA CTAGTTGCCA TGAACATGCC TCTCAACAGT 
GATGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAAATC 
AAGACAGAAG GGAACCTGGA ACAAGCTAAT GAGGAACTCC GAGCTGTGAT AAAGAAAATC 
TGGAAGAAAA CCAGCATGAA ACTGCTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAA TACCCTGCGA AGAACACCAC CATTGCCCTA 
CAGGCAGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCATGT 
GATTTGCAAG ATGACGAGCC GGAGGAAACA AAACGAGAAG AAGAAGAAGA TGGATTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGACCCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AGTTCCACCT 
GCAAGTGATA CTGAGAGACC GCTGTTTCCT CCAGCAGGAA ATTCGGGGTG TCATAGCCAT 
CATAACCATA ATTCCGTAGG AAAGCAAGCT CCCAGCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCCTGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATATG 
TCTGAAAATG GGCATCACTC CTCCCACAAG CATGACCGGG AGCCTCAAAG AAGGTCCAGT 
ATTAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGATCTG ACTCGGGAGA TGAGCAGTTC 
CCAACCATCT GCCGAGAGGA TCCGGAGCCC CACGGCTACT TCAGGGACGC CCGCTGCTTG 
GAGGAGCAGG AGTACTTCAG CGGCGAGGAG GGCTATGAGG ATGTGGGCTC TCCGGCCGGG 
GGCAGGCAGA GCTACAACTA CTACAACCGA TACCCGGGCA GCAGCTGGGA CTTCGAGCGG 
CCCCGAGGCT ACCATCACCC CCAAGGCTTC TTGGAGGATG ACGACTCGCC CGTTTGCTAT 
GACTCCCGGA GATCCCCAAG GAGACGCCTA CTACCTCCCA CCCCGACATC GCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAGGAGGT CTCGCTGTCC 
CCCACCTTCC CCCACCGCAC AGCCCTGCCA CTGCACCTGA TGCAGCAACA GATCATGGCA 
GTTGCGGGCC TGGATTCCAG TAAAGCCCAG AAGTACTCGC CGAGCCACTC AACCCGGTCG 
TGGGCCACCC CTCCAGCGAC CCCTCCGTAC CGAGACTGGA CGCCGTGCTA CGCGCCCCTG 
ATCCAGGTGG AGCGGCCAGA GGCCCTGGAC CAGGTCAACG GCAGCCTTCC ATCCCTGCAC 
CGAAGCTCAT GGTACACGGA CGAGCCCGGC ATCTCATACC GAACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGCAACAAA AACAGTGACA AGCAGAGGAG TGCAGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTAGGACGGT ATGCAAGGGA CCCAAAATTT 
GTATCGGCAA CAAAACACGA AATCGCCGAT GCCTGTGACC TGACCATCGA TGAGATGGAG 
AGCGCAGCCA GCAACCTGCT CAATGGGACT GTGCATCCCC GGGCTAACGG GGGCGTGGGC 
CCTGTCTCAC ACCGGCAGGA CTATGAGCTC CAGGACTTTG GGCCGGGCTA CAGCGATGAG 
GAGCCTGACC TCGGAAGAGA TGAGGAGGAC CTGGCAGATG AAATGATATG CATCACCACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CACNA1D
Entrez Gene ID	484724
Full Name	calcium voltage-gated channel subunit alpha1 D
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 D Names voltage-dependent L-type calcium channel subunit alpha-1D
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Bos taurus (cattle)	CACNA1D	NP_001179954.1
Rattus norvegicus (Norway rat)	Cacna1d	NP_058994.1
Gallus gallus (chicken)	CACNA1D	NP_990365.1
Homo sapiens (human)	CACNA1D	NP_000711.1
Pan troglodytes (chimpanzee)	CACNA1D	XP_001173076.3
	CACNA1D	XP_002802833.1
Mus musculus (house mouse)	Cacna1d	NP_001077085.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012434	XP_001231059.2
Canis lupus familiaris (dog)	CACNA1D	XP_849431.1
Danio rerio (zebrafish)	cacna1da	NP_982351.1
Drosophila melanogaster (fruit fly)	Ca-alpha1D	NP_001260484.1
Caenorhabditis elegans (roundworm)	egl-19	NP_001023079.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CACNA1D cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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