ACTN4 cDNA ORF clone, Canis lupus familiaris(dog)

The following ACTN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe02226 XM_541640.6
Latest version!
Canis lupus familiaris actinin alpha 4 (ACTN4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe02227 XM_005616611.3
Latest version!
Canis lupus familiaris actinin alpha 4 (ACTN4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe02228 XM_848317.5
Latest version!
Canis lupus familiaris actinin alpha 4 (ACTN4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe02229 XM_862326.5
Latest version!
Canis lupus familiaris actinin alpha 4 (ACTN4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe02230 XM_862275.5
Latest version!
Canis lupus familiaris actinin alpha 4 (ACTN4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
OCe02228 XM_848317.4 Canis lupus familiaris actinin, alpha 4 (ACTN4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe02227 XM_005616611.2 Canis lupus familiaris actinin, alpha 4 (ACTN4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe02226 XM_541640.5 Canis lupus familiaris actinin, alpha 4 (ACTN4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe02229 XM_862326.4 Canis lupus familiaris actinin, alpha 4 (ACTN4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe02230 XM_862275.4 Canis lupus familiaris actinin, alpha 4 (ACTN4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe02226
    Clone ID Related Accession (Same CDS sequence) XM_541640.6 , XM_541640.5
    Accession Version XM_541640.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-actinin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_541640.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGTGAT GCCGAGTTCA ACCGCATCAT GAGCGTGGTT
    GACCCCAACC ACAGTGGCCT CGTGACCTTC CAAGCCTTCA TTGATTTCAT GTCAAGGGAG
    ACCACCGACA CAGACACAGC TGACCAGGTC ATTGCCTCCT TCAAGGTTCT GGCAGGGGAC
    AAGAACTTCA TCACGGCTGA GGAGCTGCGG AGAGAGCTGC CCCCGGACCA GGCCGAGTAC
    TGCATCGCTC GCATGGCTCC ATACCAGGGC CCTGACGCTG TGCCCGGCGC CCTCGACTAC
    AAGTCCTTCT CCACAGCCCT GTACGGGGAG AGTGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_541640.2
    CDS86..2887
    Translation

    Target ORF information:

    RefSeq Version XM_541640.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actinin alpha 4 (ACTN4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_541640.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGTGAT GCCGAGTTCA ACCGCATCAT GAGCGTGGTT
    GACCCCAACC ACAGTGGCCT CGTGACCTTC CAAGCCTTCA TTGATTTCAT GTCAAGGGAG
    ACCACCGACA CAGACACAGC TGACCAGGTC ATTGCCTCCT TCAAGGTTCT GGCAGGGGAC
    AAGAACTTCA TCACGGCTGA GGAGCTGCGG AGAGAGCTGC CCCCGGACCA GGCCGAGTAC
    TGCATCGCTC GCATGGCTCC ATACCAGGGC CCTGACGCTG TGCCCGGCGC CCTCGACTAC
    AAGTCCTTCT CCACAGCCCT GTACGGGGAG AGTGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02226
    Clone ID Related Accession (Same CDS sequence) XM_541640.6 , XM_541640.5
    Accession Version XM_541640.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product alpha-actinin-4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_541640.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGTGAT GCCGAGTTCA ACCGCATCAT GAGCGTGGTT
    GACCCCAACC ACAGTGGCCT CGTGACCTTC CAAGCCTTCA TTGATTTCAT GTCAAGGGAG
    ACCACCGACA CAGACACAGC TGACCAGGTC ATTGCCTCCT TCAAGGTTCT GGCAGGGGAC
    AAGAACTTCA TCACGGCTGA GGAGCTGCGG AGAGAGCTGC CCCCGGACCA GGCCGAGTAC
    TGCATCGCTC GCATGGCTCC ATACCAGGGC CCTGACGCTG TGCCCGGCGC CCTCGACTAC
    AAGTCCTTCT CCACAGCCCT GTACGGGGAG AGTGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_541640.2
    CDS132..2933
    Translation

    Target ORF information:

    RefSeq Version XM_541640.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actinin, alpha 4 (ACTN4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_541640.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGTGAT GCCGAGTTCA ACCGCATCAT GAGCGTGGTT
    GACCCCAACC ACAGTGGCCT CGTGACCTTC CAAGCCTTCA TTGATTTCAT GTCAAGGGAG
    ACCACCGACA CAGACACAGC TGACCAGGTC ATTGCCTCCT TCAAGGTTCT GGCAGGGGAC
    AAGAACTTCA TCACGGCTGA GGAGCTGCGG AGAGAGCTGC CCCCGGACCA GGCCGAGTAC
    TGCATCGCTC GCATGGCTCC ATACCAGGGC CCTGACGCTG TGCCCGGCGC CCTCGACTAC
    AAGTCCTTCT CCACAGCCCT GTACGGGGAG AGTGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02227
    Clone ID Related Accession (Same CDS sequence) XM_005616611.3 , XM_005616611.2
    Accession Version XM_005616611.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-actinin-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005616611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCGGGG GCTCAAAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGTGAT GCCGAGTTCA ACCGCATCAT GAGCGTGGTT
    GACCCCAACC ACAGTGGCCT CGTGACCTTC CAAGCCTTCA TTGATTTCAT GTCAAGGGAG
    ACCACCGACA CAGACACAGC TGACCAGGTC ATTGCCTCCT TCAAGGTTCT GGCAGGGGAC
    AAGAACTTCA TCACGGCTGA GGAGCTGCGG AGAGAGCTGC CCCCGGACCA GGCCGAGTAC
    TGCATCGCTC GCATGGCTCC ATACCAGGGC CCTGACGCTG TGCCCGGCGC CCTCGACTAC
    AAGTCCTTCT CCACAGCCCT GTACGGGGAG AGTGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616668.1
    CDS86..2887
    Translation

    Target ORF information:

    RefSeq Version XM_005616611.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actinin alpha 4 (ACTN4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616611.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCGGGG GCTCAAAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGTGAT GCCGAGTTCA ACCGCATCAT GAGCGTGGTT
    GACCCCAACC ACAGTGGCCT CGTGACCTTC CAAGCCTTCA TTGATTTCAT GTCAAGGGAG
    ACCACCGACA CAGACACAGC TGACCAGGTC ATTGCCTCCT TCAAGGTTCT GGCAGGGGAC
    AAGAACTTCA TCACGGCTGA GGAGCTGCGG AGAGAGCTGC CCCCGGACCA GGCCGAGTAC
    TGCATCGCTC GCATGGCTCC ATACCAGGGC CCTGACGCTG TGCCCGGCGC CCTCGACTAC
    AAGTCCTTCT CCACAGCCCT GTACGGGGAG AGTGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02227
    Clone ID Related Accession (Same CDS sequence) XM_005616611.3 , XM_005616611.2
    Accession Version XM_005616611.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product alpha-actinin-4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616611.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCGGGG GCTCAAAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGTGAT GCCGAGTTCA ACCGCATCAT GAGCGTGGTT
    GACCCCAACC ACAGTGGCCT CGTGACCTTC CAAGCCTTCA TTGATTTCAT GTCAAGGGAG
    ACCACCGACA CAGACACAGC TGACCAGGTC ATTGCCTCCT TCAAGGTTCT GGCAGGGGAC
    AAGAACTTCA TCACGGCTGA GGAGCTGCGG AGAGAGCTGC CCCCGGACCA GGCCGAGTAC
    TGCATCGCTC GCATGGCTCC ATACCAGGGC CCTGACGCTG TGCCCGGCGC CCTCGACTAC
    AAGTCCTTCT CCACAGCCCT GTACGGGGAG AGTGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616668.1
    CDS132..2933
    Translation

    Target ORF information:

    RefSeq Version XM_005616611.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actinin, alpha 4 (ACTN4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616611.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCGGGG GCTCAAAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGTGAT GCCGAGTTCA ACCGCATCAT GAGCGTGGTT
    GACCCCAACC ACAGTGGCCT CGTGACCTTC CAAGCCTTCA TTGATTTCAT GTCAAGGGAG
    ACCACCGACA CAGACACAGC TGACCAGGTC ATTGCCTCCT TCAAGGTTCT GGCAGGGGAC
    AAGAACTTCA TCACGGCTGA GGAGCTGCGG AGAGAGCTGC CCCCGGACCA GGCCGAGTAC
    TGCATCGCTC GCATGGCTCC ATACCAGGGC CCTGACGCTG TGCCCGGCGC CCTCGACTAC
    AAGTCCTTCT CCACAGCCCT GTACGGGGAG AGTGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02228
    Clone ID Related Accession (Same CDS sequence) XM_848317.5 , XM_848317.4
    Accession Version XM_848317.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-actinin-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_848317.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGGG TGATGCCGAG TTCAACCGCA TCATGAGCGT GGTTGACCCC
    AACCACAGTG GCCTCGTGAC CTTCCAAGCC TTCATTGATT TCATGTCAAG GGAGACCACC
    GACACAGACA CAGCTGACCA GGTCATTGCC TCCTTCAAGG TTCTGGCAGG GGACAAGAAC
    TTCATCACGG CTGAGGAGCT GCGGAGAGAG CTGCCCCCGG ACCAGGCCGA GTACTGCATC
    GCTCGCATGG CTCCATACCA GGGCCCTGAC GCTGTGCCCG GCGCCCTCGA CTACAAGTCC
    TTCTCCACAG CCCTGTACGG GGAGAGTGAC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_853410.1
    CDS85..2820
    Translation

    Target ORF information:

    RefSeq Version XM_848317.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actinin alpha 4 (ACTN4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_848317.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGGG TGATGCCGAG TTCAACCGCA TCATGAGCGT GGTTGACCCC
    AACCACAGTG GCCTCGTGAC CTTCCAAGCC TTCATTGATT TCATGTCAAG GGAGACCACC
    GACACAGACA CAGCTGACCA GGTCATTGCC TCCTTCAAGG TTCTGGCAGG GGACAAGAAC
    TTCATCACGG CTGAGGAGCT GCGGAGAGAG CTGCCCCCGG ACCAGGCCGA GTACTGCATC
    GCTCGCATGG CTCCATACCA GGGCCCTGAC GCTGTGCCCG GCGCCCTCGA CTACAAGTCC
    TTCTCCACAG CCCTGTACGG GGAGAGTGAC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02228
    Clone ID Related Accession (Same CDS sequence) XM_848317.5 , XM_848317.4
    Accession Version XM_848317.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product alpha-actinin-4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_848317.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGGG TGATGCCGAG TTCAACCGCA TCATGAGCGT GGTTGACCCC
    AACCACAGTG GCCTCGTGAC CTTCCAAGCC TTCATTGATT TCATGTCAAG GGAGACCACC
    GACACAGACA CAGCTGACCA GGTCATTGCC TCCTTCAAGG TTCTGGCAGG GGACAAGAAC
    TTCATCACGG CTGAGGAGCT GCGGAGAGAG CTGCCCCCGG ACCAGGCCGA GTACTGCATC
    GCTCGCATGG CTCCATACCA GGGCCCTGAC GCTGTGCCCG GCGCCCTCGA CTACAAGTCC
    TTCTCCACAG CCCTGTACGG GGAGAGTGAC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_853410.1
    CDS1..2736
    Translation

    Target ORF information:

    RefSeq Version XM_848317.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actinin, alpha 4 (ACTN4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_848317.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAATG ACCGGCAGGG TGATGCCGAG TTCAACCGCA TCATGAGCGT GGTTGACCCC
    AACCACAGTG GCCTCGTGAC CTTCCAAGCC TTCATTGATT TCATGTCAAG GGAGACCACC
    GACACAGACA CAGCTGACCA GGTCATTGCC TCCTTCAAGG TTCTGGCAGG GGACAAGAAC
    TTCATCACGG CTGAGGAGCT GCGGAGAGAG CTGCCCCCGG ACCAGGCCGA GTACTGCATC
    GCTCGCATGG CTCCATACCA GGGCCCTGAC GCTGTGCCCG GCGCCCTCGA CTACAAGTCC
    TTCTCCACAG CCCTGTACGG GGAGAGTGAC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02229
    Clone ID Related Accession (Same CDS sequence) XM_862326.4 , XM_862326.5
    Accession Version XM_862326.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product alpha-actinin-4 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_862326.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGTGATG CCGAGTTCAA CCGCATCATG AGCGTGGTTG ACCCCAACCA CAGTGGCCTC
    GTGACCTTCC AAGCCTTCAT TGATTTCATG TCAAGGGAGA CCACCGACAC AGACACAGCT
    GACCAGGTCA TTGCCTCCTT CAAGGTTCTG GCAGGGGACA AGAACTTCAT CACGGCTGAG
    GAGCTGCGGA GAGAGCTGCC CCCGGACCAG GCCGAGTACT GCATCGCTCG CATGGCTCCA
    TACCAGGGCC CTGACGCTGT GCCCGGCGCC CTCGACTACA AGTCCTTCTC CACAGCCCTG
    TACGGGGAGA GTGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_867419.1
    CDS132..2852
    Translation

    Target ORF information:

    RefSeq Version XM_862326.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actinin, alpha 4 (ACTN4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_862326.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGTGATG CCGAGTTCAA CCGCATCATG AGCGTGGTTG ACCCCAACCA CAGTGGCCTC
    GTGACCTTCC AAGCCTTCAT TGATTTCATG TCAAGGGAGA CCACCGACAC AGACACAGCT
    GACCAGGTCA TTGCCTCCTT CAAGGTTCTG GCAGGGGACA AGAACTTCAT CACGGCTGAG
    GAGCTGCGGA GAGAGCTGCC CCCGGACCAG GCCGAGTACT GCATCGCTCG CATGGCTCCA
    TACCAGGGCC CTGACGCTGT GCCCGGCGCC CTCGACTACA AGTCCTTCTC CACAGCCCTG
    TACGGGGAGA GTGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02229
    Clone ID Related Accession (Same CDS sequence) XM_862326.4 , XM_862326.5
    Accession Version XM_862326.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-actinin-4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_862326.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGTGATG CCGAGTTCAA CCGCATCATG AGCGTGGTTG ACCCCAACCA CAGTGGCCTC
    GTGACCTTCC AAGCCTTCAT TGATTTCATG TCAAGGGAGA CCACCGACAC AGACACAGCT
    GACCAGGTCA TTGCCTCCTT CAAGGTTCTG GCAGGGGACA AGAACTTCAT CACGGCTGAG
    GAGCTGCGGA GAGAGCTGCC CCCGGACCAG GCCGAGTACT GCATCGCTCG CATGGCTCCA
    TACCAGGGCC CTGACGCTGT GCCCGGCGCC CTCGACTACA AGTCCTTCTC CACAGCCCTG
    TACGGGGAGA GTGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_867419.1
    CDS86..2806
    Translation

    Target ORF information:

    RefSeq Version XM_862326.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actinin alpha 4 (ACTN4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_862326.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGTGATG CCGAGTTCAA CCGCATCATG AGCGTGGTTG ACCCCAACCA CAGTGGCCTC
    GTGACCTTCC AAGCCTTCAT TGATTTCATG TCAAGGGAGA CCACCGACAC AGACACAGCT
    GACCAGGTCA TTGCCTCCTT CAAGGTTCTG GCAGGGGACA AGAACTTCAT CACGGCTGAG
    GAGCTGCGGA GAGAGCTGCC CCCGGACCAG GCCGAGTACT GCATCGCTCG CATGGCTCCA
    TACCAGGGCC CTGACGCTGT GCCCGGCGCC CTCGACTACA AGTCCTTCTC CACAGCCCTG
    TACGGGGAGA GTGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02230
    Clone ID Related Accession (Same CDS sequence) XM_862275.4 , XM_862275.5
    Accession Version XM_862275.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product alpha-actinin-4 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_862275.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCGGGG GCTCAAAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGTGATG CCGAGTTCAA CCGCATCATG AGCGTGGTTG ACCCCAACCA CAGTGGCCTC
    GTGACCTTCC AAGCCTTCAT TGATTTCATG TCAAGGGAGA CCACCGACAC AGACACAGCT
    GACCAGGTCA TTGCCTCCTT CAAGGTTCTG GCAGGGGACA AGAACTTCAT CACGGCTGAG
    GAGCTGCGGA GAGAGCTGCC CCCGGACCAG GCCGAGTACT GCATCGCTCG CATGGCTCCA
    TACCAGGGCC CTGACGCTGT GCCCGGCGCC CTCGACTACA AGTCCTTCTC CACAGCCCTG
    TACGGGGAGA GTGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_867368.1
    CDS132..2852
    Translation

    Target ORF information:

    RefSeq Version XM_862275.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actinin, alpha 4 (ACTN4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_862275.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCGGGG GCTCAAAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGTGATG CCGAGTTCAA CCGCATCATG AGCGTGGTTG ACCCCAACCA CAGTGGCCTC
    GTGACCTTCC AAGCCTTCAT TGATTTCATG TCAAGGGAGA CCACCGACAC AGACACAGCT
    GACCAGGTCA TTGCCTCCTT CAAGGTTCTG GCAGGGGACA AGAACTTCAT CACGGCTGAG
    GAGCTGCGGA GAGAGCTGCC CCCGGACCAG GCCGAGTACT GCATCGCTCG CATGGCTCCA
    TACCAGGGCC CTGACGCTGT GCCCGGCGCC CTCGACTACA AGTCCTTCTC CACAGCCCTG
    TACGGGGAGA GTGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02230
    Clone ID Related Accession (Same CDS sequence) XM_862275.4 , XM_862275.5
    Accession Version XM_862275.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-actinin-4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_862275.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCGGGG GCTCAAAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGTGATG CCGAGTTCAA CCGCATCATG AGCGTGGTTG ACCCCAACCA CAGTGGCCTC
    GTGACCTTCC AAGCCTTCAT TGATTTCATG TCAAGGGAGA CCACCGACAC AGACACAGCT
    GACCAGGTCA TTGCCTCCTT CAAGGTTCTG GCAGGGGACA AGAACTTCAT CACGGCTGAG
    GAGCTGCGGA GAGAGCTGCC CCCGGACCAG GCCGAGTACT GCATCGCTCG CATGGCTCCA
    TACCAGGGCC CTGACGCTGT GCCCGGCGCC CTCGACTACA AGTCCTTCTC CACAGCCCTG
    TACGGGGAGA GTGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_867368.1
    CDS86..2806
    Translation

    Target ORF information:

    RefSeq Version XM_862275.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actinin alpha 4 (ACTN4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_862275.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGGGCAAT 
    GGCGCCGGCG GCGGGGGAAG CATGGGCGAC TACATGGCCC AGGAAGACGA CTGGGACCGG
    GACCTGCTGC TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACATTCAC AGCCTGGTGC
    AACTCCCACC TGAGGAAGGC TGGCACACAG ATAGAGAACA TCGACGAGGA CTTTCGAGAT
    GGGCTCAAGC TCATGCTTCT CCTGGAGGTC ATTTCAGGAG AGCGGTTACC TAAGCCGGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AATGTGAACA AAGCCCTGGA CTTCATCGCC
    AGCAAAGGAG TCAAGCTGGT CTCCATCGGG GCAGAAGAGA TTGTGGACGG CAATGCAAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA AGGGCTGCTC CTGTGGTGCC AGAGGAAGAC GGCGCCATAT
    AAGAACGTCA ACGTGCAGAA TTTCCACATC AGCTGGAAGG ACGGTCTTGC CTTCAATGCA
    CTGATCCACC GGCACAGACC AGAGCTGATC GAATATGACA AGCTGAGAAA GGACGACCCT
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCCGAGAAAT ACCTGGACAT TCCCAAGATG
    TTGGATGCGG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCGGGG GCTCAAAAGG CCGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCTGT CAACCAGGAG AATGAACACC TCATGGAAGA TTATGAGAGG
    CTGGCCAGTG ACCTCCTGGA GTGGATCCGG CGCACCATCC CGTGGCTGGA GGACCGCGTG
    CCGCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGGGA CTACCGGCGC
    GTCCATAAGC CACCCAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACACTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCC GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA TGAGGAGTGG
    CTGCTGAATG AGATCCGCAG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAC GGGAAGGAAG CCATGCTGAA GCAGCGGGAC
    TATGAGACGG CCACCCTGTC GGATATTAAA GCTCTCATTC GCAAGCACGA GGCCTTTGAG
    AGTGACCTGG CCGCCCACCA GGACCGCGTG GAGCAAATCG CAGCAATTGC TCAGGAGCTC
    AATGAGCTGG ACTACTATGA CTCCCACAAT GTCAACACCC GGTGCCAGAA GATCTGTGAC
    CAGTGGGATG CTCTCGGCTC CCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTGG AGACCATCGA CCAGCTACAC TTGGAATATG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGTGC CATGGAGGAC CTCCAGGACA TGTTCATTGT CCACACCATC
    GAGGAGATTG AGGGCCTGAT TTCAGCCCAC GACCAGTTCA AGTCGACACT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAGG CCCAGAGGAT CGCCGAGAGC
    AACCACATCA AACTGTCGGG CAGTAACCCC TACACCACCG TCACCCCGCA GATCATCAAC
    TCTAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAA
    CAGAGCAAGC AGCAGTCCAA TGAGCACCTC CGCCGCCAGT TTGCCAGCCA GGCCAACATC
    GTGGGGCCCT GGATCCAGAC TAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC
    GGGACCCTGG AGGACCAGCT GAACCATCTG AAACGGTACG AGCGCAGCAT CGTGGACTAC
    AAGCCGAACC TGGATCTGCT TGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA CACCATGGAG CACATCCGTG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACGAT CAACGAGGTC GAGAACCAGA TCCTTACCCG TGATGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCATCCT TCAATCACTT TGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGTGATG CCGAGTTCAA CCGCATCATG AGCGTGGTTG ACCCCAACCA CAGTGGCCTC
    GTGACCTTCC AAGCCTTCAT TGATTTCATG TCAAGGGAGA CCACCGACAC AGACACAGCT
    GACCAGGTCA TTGCCTCCTT CAAGGTTCTG GCAGGGGACA AGAACTTCAT CACGGCTGAG
    GAGCTGCGGA GAGAGCTGCC CCCGGACCAG GCCGAGTACT GCATCGCTCG CATGGCTCCA
    TACCAGGGCC CTGACGCTGT GCCCGGCGCC CTCGACTACA AGTCCTTCTC CACAGCCCTG
    TACGGGGAGA GTGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.