IARS cDNA ORF clone, Canis lupus familiaris(Dog)

The following IARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe286113 XM_022422371.1
Latest version!
Canis lupus familiaris isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe01491 XM_014118130.2
Latest version!
Canis lupus familiaris isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe01491 XM_541332.6
Latest version!
Canis lupus familiaris isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OCe01491 XM_014118130.1 Canis lupus familiaris isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe01491 XM_541332.5 Canis lupus familiaris isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe286113
    Clone ID Related Accession (Same CDS sequence) XM_022422371.1
    Accession Version XM_022422371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTCTGGTCCG AATTTAATTG TTTTCAAGAA TGCTTAACGC AATCTAAACA TAAGCCAAAA
    TTTACATTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACCAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAAGA TGTAGCCAAA ATGGGGATTG TAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG
    TGGGTCTTCA AACAGCTCTA TGACAAAGGC CTTGTATATA GAGGCGTGAA AGTCATGCCT
    TTCTCCACCG CGTGCAACAC TCCACTGTCC AACTTCGAGT CTCACCAGAA CTACAAGGAT
    GTTCAAGACC CTTCAGTGTT TATAACTTTC CCTTTGGAGG AAGACGAAAA TGTGTCTTTG
    GTGGCCTGGA CAACCACTCC TTGGACTCTG CCTAGTAACC TGGCCCTGTG TGTTAATCCA
    GAAATGCAGT ATGTGAAGAT TAAAGATGTC ACCAGAGGAA AGTTACTCAT CTTGATGGAA
    GCCAGATTAC TAGCCCTCTA TAAGGTGGAG ACAGACTACG AGATCCTCGA AAGATTTCCC
    GGTAGCTATC TCAAAGGCAA GAAGTACAGG CCGCTGTTTG ACTACTTTGT GCAGCTCAAG
    GAGAATGGTG CTTTCACGGT GCTTGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACCGGA
    GTGGTACACC AGGCTCCTTA CTTCGGGGCT GACGACTATC GGGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCTCC CCCTGTTTGC CCTGTGGATG CCTCAGGCTG TTTCACAGCA
    GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT AATCAGGACC
    CTGAAGGAAA GAGGCCGGCT TCTCATTGCC AGCACCCTCA CTCACAGCTA CCCGTTCTGC
    TGGAGGTCAG ATACTCCCCT GATTTACAAG GCTGTGCCTA GCTGGTTCGT GCGCGTGGAG
    CACATGGTGG ACCAACTCCT GCAGAACAAC GACCTGTGTT ACTGGGTCCC AGAGTTCGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC
    AGATACTGGG GCACGCCCAT CCCCCTGTGG GTCAGCGATG ACTTCGAGGA GGTAGTCTGC
    ATCGGGTCGG TGGCAGAACT GGAAGAACTG ACGGGAGCAA AGGTCTCTGA TCTCCACAGA
    GAGAGTATTG ACCACCTGAC CATCCCTTCT CACTGCGGGA AAGGAGTGTT GCGGCGCATC
    TCCGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT
    CCTTTTGAAA ACAAAAGGGA GTTTGATGAT GCGTTCCCTG CAGACTTCAT CGCTGAAGGC
    ATTGACCAGA CCAGAGGATG GTTTTACACC TTGCTGGTGC TGGCCACTGC TCTCTTTGGA
    CAGCCGCCTT TCAGGAACGT GATTGTGAAC GGGCTGGTCC TGGCAAGTGA TGGTCAGAAG
    ATGAGCAAAC GAAAAAAGAA TTATCCGGAT CCACTTTCAA TCATCCATAA ATACGGTGCC
    GACGCCCTCA GATTATATCT GATCAACTCC CCAGTGGTGA GAGCAGAAAA CCTGCGATTT
    AAGGAGGAGG GTGTGCGTGA TGTCCTGAAG GATGTGCTGC TCCCTTGGTA CAATGCCTAC
    CGTTTCTTCA TCCAGAACGT GCTGAGGCTG CAGAAGGAAG AAGAAATGGA ATTCATCTAC
    AACGAGAACA CAGCTAGAGA AAGCGCTAAC GTCACAGACC GGTGGGTCCT GTCCTGCATG
    CAGTCGCTGG TTGGGTTCTT TGAGACGGAA ATGGCAGCCT ACAGGCTGTA TACCGTGGTA
    CCTCGCCTGG TCAAGTTTGT GGATGTCCTC ACCAACTGGT ACGTCAGGAT GAACCGCAGA
    AGGTTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACGG CCCTGGAGAC CTTGTTCAGT
    GTCCTGCTGT CTCTGTGCAG ACTGATGGCC CCCTATACAC CTTTCCTCAC CGAGTTGATG
    TACCAGAATC TAAAGCTGCT TATCGACCCT GTTTCTGTCC AGGACAAGGA CACACAGAGC
    ATCCACTACC TCATGCTGCC CCACGTTCGA GAGGAGCTGA TTGACAAGAG CACGGAGAGC
    GCAGTGTCTC GGATGCAGTC TGTGATTGAA CTGGGACGAG TGATTCGAGA CCGCAGAACC
    ATTCCAATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAGGATCC GGAAGCTCTT
    CATGAGATCA GGTCTCTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AGAGGTTACC
    TTGTCTACAG ATAAAAACAA GTATGGCATT CGCCTCAGGG CAGAACCTGA TCACATGGTC
    CTAGGGAAGC GTCTGAAGGG AGCCTTCCGG GCAGTCATGG CGGCCATCAA GCAGCTGAGC
    AGTGAGGAGC TGGAGTGCTT CCAGGAGAGT GGGACCATTG TTGTCGAAGG CCATGAGCTG
    CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA AAACAGGAGG GACGACGCAG
    TATGAAGCAC ACTCAGATGC CCAGGCTTTG GTTCTCTTAG ATGTCACCCC CGACCAGTCA
    ATGGTAGATG AAGGAGTGGC TCGGGAGGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCGACTGA TGAAATAACA GTTTATTATA AAGCAAAGTC TGAAGGGAAG
    TATCTGAATA ATGTTATCGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAAGCTCCC
    TTGAAACCGT ATCCAGTTCC AGTGTCTGAT AAAATCATTA TTCAAGAAGA AATGCAGTTA
    AAGGGTTCTG CCCTGGAAAT TACACTCACC AGAGGATCCT CCCTGCCTGG TCCTGTTTGT
    GCATATGTCA ATCTTAACAT TTATGCAAAT GGCAGCGAGC AAGAAATACA AAACCAGACT
    GATTTACTGA GTATTAGTGG AAAAACACTT CGCGTGGCGG CAGGGTCAGC CCCCGCTGTA
    GTGGACGGCC CCAGCACTGC ACTCTGCCGT TACATCAACC TACAGCTCCT GAACGCAGGC
    CCGCAAGAGT GTGTGAAGGG AACAGTGGGC ACACTCCTGC TGGAAAACCC GCCTGGGCAG
    AATGGACTTA CCCACCAGGG CCTCCTGTAT GAAGCAGCCA AGGTGTTTGG TCTTCGCAGC
    AGGAAGCTCA AGCTGTTTCT GAACGAGACT CAGACACATG AAATTACAGA AGAAATCCCC
    ATGAAGGCTT TGAACATGAA GACTGTGTAT GTTTCTGTAT TACCGACGAC GGCAGACTTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022278079.1
    CDS144..3806
    Translation

    Target ORF information:

    RefSeq Version XM_022422371.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022422371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTCTGGTCCG AATTTAATTG TTTTCAAGAA TGCTTAACGC AATCTAAACA TAAGCCAAAA
    TTTACATTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACCAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAAGA TGTAGCCAAA ATGGGGATTG TAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG
    TGGGTCTTCA AACAGCTCTA TGACAAAGGC CTTGTATATA GAGGCGTGAA AGTCATGCCT
    TTCTCCACCG CGTGCAACAC TCCACTGTCC AACTTCGAGT CTCACCAGAA CTACAAGGAT
    GTTCAAGACC CTTCAGTGTT TATAACTTTC CCTTTGGAGG AAGACGAAAA TGTGTCTTTG
    GTGGCCTGGA CAACCACTCC TTGGACTCTG CCTAGTAACC TGGCCCTGTG TGTTAATCCA
    GAAATGCAGT ATGTGAAGAT TAAAGATGTC ACCAGAGGAA AGTTACTCAT CTTGATGGAA
    GCCAGATTAC TAGCCCTCTA TAAGGTGGAG ACAGACTACG AGATCCTCGA AAGATTTCCC
    GGTAGCTATC TCAAAGGCAA GAAGTACAGG CCGCTGTTTG ACTACTTTGT GCAGCTCAAG
    GAGAATGGTG CTTTCACGGT GCTTGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACCGGA
    GTGGTACACC AGGCTCCTTA CTTCGGGGCT GACGACTATC GGGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCTCC CCCTGTTTGC CCTGTGGATG CCTCAGGCTG TTTCACAGCA
    GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT AATCAGGACC
    CTGAAGGAAA GAGGCCGGCT TCTCATTGCC AGCACCCTCA CTCACAGCTA CCCGTTCTGC
    TGGAGGTCAG ATACTCCCCT GATTTACAAG GCTGTGCCTA GCTGGTTCGT GCGCGTGGAG
    CACATGGTGG ACCAACTCCT GCAGAACAAC GACCTGTGTT ACTGGGTCCC AGAGTTCGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC
    AGATACTGGG GCACGCCCAT CCCCCTGTGG GTCAGCGATG ACTTCGAGGA GGTAGTCTGC
    ATCGGGTCGG TGGCAGAACT GGAAGAACTG ACGGGAGCAA AGGTCTCTGA TCTCCACAGA
    GAGAGTATTG ACCACCTGAC CATCCCTTCT CACTGCGGGA AAGGAGTGTT GCGGCGCATC
    TCCGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT
    CCTTTTGAAA ACAAAAGGGA GTTTGATGAT GCGTTCCCTG CAGACTTCAT CGCTGAAGGC
    ATTGACCAGA CCAGAGGATG GTTTTACACC TTGCTGGTGC TGGCCACTGC TCTCTTTGGA
    CAGCCGCCTT TCAGGAACGT GATTGTGAAC GGGCTGGTCC TGGCAAGTGA TGGTCAGAAG
    ATGAGCAAAC GAAAAAAGAA TTATCCGGAT CCACTTTCAA TCATCCATAA ATACGGTGCC
    GACGCCCTCA GATTATATCT GATCAACTCC CCAGTGGTGA GAGCAGAAAA CCTGCGATTT
    AAGGAGGAGG GTGTGCGTGA TGTCCTGAAG GATGTGCTGC TCCCTTGGTA CAATGCCTAC
    CGTTTCTTCA TCCAGAACGT GCTGAGGCTG CAGAAGGAAG AAGAAATGGA ATTCATCTAC
    AACGAGAACA CAGCTAGAGA AAGCGCTAAC GTCACAGACC GGTGGGTCCT GTCCTGCATG
    CAGTCGCTGG TTGGGTTCTT TGAGACGGAA ATGGCAGCCT ACAGGCTGTA TACCGTGGTA
    CCTCGCCTGG TCAAGTTTGT GGATGTCCTC ACCAACTGGT ACGTCAGGAT GAACCGCAGA
    AGGTTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACGG CCCTGGAGAC CTTGTTCAGT
    GTCCTGCTGT CTCTGTGCAG ACTGATGGCC CCCTATACAC CTTTCCTCAC CGAGTTGATG
    TACCAGAATC TAAAGCTGCT TATCGACCCT GTTTCTGTCC AGGACAAGGA CACACAGAGC
    ATCCACTACC TCATGCTGCC CCACGTTCGA GAGGAGCTGA TTGACAAGAG CACGGAGAGC
    GCAGTGTCTC GGATGCAGTC TGTGATTGAA CTGGGACGAG TGATTCGAGA CCGCAGAACC
    ATTCCAATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAGGATCC GGAAGCTCTT
    CATGAGATCA GGTCTCTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AGAGGTTACC
    TTGTCTACAG ATAAAAACAA GTATGGCATT CGCCTCAGGG CAGAACCTGA TCACATGGTC
    CTAGGGAAGC GTCTGAAGGG AGCCTTCCGG GCAGTCATGG CGGCCATCAA GCAGCTGAGC
    AGTGAGGAGC TGGAGTGCTT CCAGGAGAGT GGGACCATTG TTGTCGAAGG CCATGAGCTG
    CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA AAACAGGAGG GACGACGCAG
    TATGAAGCAC ACTCAGATGC CCAGGCTTTG GTTCTCTTAG ATGTCACCCC CGACCAGTCA
    ATGGTAGATG AAGGAGTGGC TCGGGAGGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCGACTGA TGAAATAACA GTTTATTATA AAGCAAAGTC TGAAGGGAAG
    TATCTGAATA ATGTTATCGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAAGCTCCC
    TTGAAACCGT ATCCAGTTCC AGTGTCTGAT AAAATCATTA TTCAAGAAGA AATGCAGTTA
    AAGGGTTCTG CCCTGGAAAT TACACTCACC AGAGGATCCT CCCTGCCTGG TCCTGTTTGT
    GCATATGTCA ATCTTAACAT TTATGCAAAT GGCAGCGAGC AAGAAATACA AAACCAGACT
    GATTTACTGA GTATTAGTGG AAAAACACTT CGCGTGGCGG CAGGGTCAGC CCCCGCTGTA
    GTGGACGGCC CCAGCACTGC ACTCTGCCGT TACATCAACC TACAGCTCCT GAACGCAGGC
    CCGCAAGAGT GTGTGAAGGG AACAGTGGGC ACACTCCTGC TGGAAAACCC GCCTGGGCAG
    AATGGACTTA CCCACCAGGG CCTCCTGTAT GAAGCAGCCA AGGTGTTTGG TCTTCGCAGC
    AGGAAGCTCA AGCTGTTTCT GAACGAGACT CAGACACATG AAATTACAGA AGAAATCCCC
    ATGAAGGCTT TGAACATGAA GACTGTGTAT GTTTCTGTAT TACCGACGAC GGCAGACTTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01491
    Clone ID Related Accession (Same CDS sequence) XM_014118130.2 , XM_014118130.1 , XM_541332.6 , XM_541332.5
    Accession Version XM_014118130.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTCTGGTCCG AATTTAATTG TTTTCAAGAA TGCTTAACGC AATCTAAACA TAAGCCAAAA
    TTTACATTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACCAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAAGA TGTAGCCAAA ATGGGGATTG TAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG
    TGGGTCTTCA AACAGCTCTA TGACAAAGGC CTTGTATATA GAGGCGTGAA AGTCATGCCT
    TTCTCCACCG CGTGCAACAC TCCACTGTCC AACTTCGAGT CTCACCAGAA CTACAAGGAT
    GTTCAAGACC CTTCAGTGTT TATAACTTTC CCTTTGGAGG AAGACGAAAA TGTGTCTTTG
    GTGGCCTGGA CAACCACTCC TTGGACTCTG CCTAGTAACC TGGCCCTGTG TGTTAATCCA
    GAAATGCAGT ATGTGAAGAT TAAAGATGTC ACCAGAGGAA AGTTACTCAT CTTGATGGAA
    GCCAGATTAC TAGCCCTCTA TAAGGTGGAG ACAGACTACG AGATCCTCGA AAGATTTCCC
    GGTAGCTATC TCAAAGGCAA GAAGTACAGG CCGCTGTTTG ACTACTTTGT GCAGCTCAAG
    GAGAATGGTG CTTTCACGGT GCTTGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACCGGA
    GTGGTACACC AGGCTCCTTA CTTCGGGGCT GACGACTATC GGGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCTCC CCCTGTTTGC CCTGTGGATG CCTCAGGCTG TTTCACAGCA
    GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT AATCAGGACC
    CTGAAGGAAA GAGGCCGGCT TCTCATTGCC AGCACCCTCA CTCACAGCTA CCCGTTCTGC
    TGGAGGTCAG ATACTCCCCT GATTTACAAG GCTGTGCCTA GCTGGTTCGT GCGCGTGGAG
    CACATGGTGG ACCAACTCCT GCAGAACAAC GACCTGTGTT ACTGGGTCCC AGAGTTCGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC
    AGATACTGGG GCACGCCCAT CCCCCTGTGG GTCAGCGATG ACTTCGAGGA GGTAGTCTGC
    ATCGGGTCGG TGGCAGAACT GGAAGAACTG ACGGGAGCAA AGGTCTCTGA TCTCCACAGA
    GAGAGTATTG ACCACCTGAC CATCCCTTCT CACTGCGGGA AAGGAGTGTT GCGGCGCATC
    TCCGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT
    CCTTTTGAAA ACAAAAGGGA GTTTGATGAT GCGTTCCCTG CAGACTTCAT CGCTGAAGGC
    ATTGACCAGA CCAGAGGATG GTTTTACACC TTGCTGGTGC TGGCCACTGC TCTCTTTGGA
    CAGCCGCCTT TCAGGAACGT GATTGTGAAC GGGCTGGTCC TGGCAAGTGA TGGTCAGAAG
    ATGAGCAAAC GAAAAAAGAA TTATCCGGAT CCACTTTCAA TCATCCATAA ATACGGTGCC
    GACGCCCTCA GATTATATCT GATCAACTCC CCAGTGGTGA GAGCAGAAAA CCTGCGATTT
    AAGGAGGAGG GTGTGCGTGA TGTCCTGAAG GATGTGCTGC TCCCTTGGTA CAATGCCTAC
    CGTTTCTTCA TCCAGAACGT GCTGAGGCTG CAGAAGGAAG AAGAAATGGA ATTCATCTAC
    AACGAGAACA CAGCTAGAGA AAGCGCTAAC GTCACAGACC GGTGGGTCCT GTCCTGCATG
    CAGTCGCTGG TTGGGTTCTT TGAGACGGAA ATGGCAGCCT ACAGGCTGTA TACCGTGGTA
    CCTCGCCTGG TCAAGTTTGT GGATGTCCTC ACCAACTGGT ACGTCAGGAT GAACCGCAGA
    AGGTTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACGG CCCTGGAGAC CTTGTTCAGT
    GTCCTGCTGT CTCTGTGCAG ACTGATGGCC CCCTATACAC CTTTCCTCAC CGAGTTGATG
    TACCAGAATC TAAAGCTGCT TATCGACCCT GTTTCTGTCC AGGACAAGGA CACACAGAGC
    ATCCACTACC TCATGCTGCC CCACGTTCGA GAGGAGCTGA TTGACAAGAG CACGGAGAGC
    GCAGTGTCTC GGATGCAGTC TGTGATTGAA CTGGGACGAG TGATTCGAGA CCGCAGAACC
    ATTCCAATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAGGATCC GGAAGCTCTT
    CATGAGATCA GGTCTCTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AGAGGTTACC
    TTGTCTACAG ATAAAAACAA GTATGGCATT CGCCTCAGGG CAGAACCTGA TCACATGGTC
    CTAGGGAAGC GTCTGAAGGG AGCCTTCCGG GCAGTCATGG CGGCCATCAA GCAGCTGAGC
    AGTGAGGAGC TGGAGTGCTT CCAGGAGAGT GGGACCATTG TTGTCGAAGG CCATGAGCTG
    CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA AAACAGGAGG GACGACGCAG
    TATGAAGCAC ACTCAGATGC CCAGGCTTTG GTTCTCTTAG ATGTCACCCC CGACCAGTCA
    ATGGTAGATG AAGGAGTGGC TCGGGAGGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCGACTGA TGAAATAACA GTTTATTATA AAGCAAAGTC TGAAGGGAAG
    TATCTGAATA ATGTTATCGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAAGCTCCC
    TTGAAACCGT ATCCAGTTCC AGTGTCTGAT AAAATCATTA TTCAAGAAGA AATGCAGTTA
    AAGGGTTCTG CCCTGGAAAT TACACTCACC AGAGGATCCT CCCTGCCTGG TCCTGTTTGT
    GCATATGTCA ATCTTAACAT TTATGCAAAT GGCAGCGAGC AAGGTGGAGT ATTGCTTCTG
    GAAAACCCAA AAGGCGATAA CAGGTTGGAT GTTTTAAAGC TGAAGAGCAT CATTACTAGT
    ATTTTTGGTG TACGGAATTC TGAGCTGGCT GTCCTCCATG GTGCAGTGGA AATACAAAAC
    CAGACTGATT TACTGAGTAT TAGTGGAAAA ACACTTCGCG TGGCGGCAGG GTCAGCCCCC
    GCTGTAGTGG ACGGCCCCAG CACTGCACTC TGCCGTTACA TCAACCTACA GCTCCTGAAC
    GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACAC TCCTGCTGGA AAACCCGCCT
    GGGCAGAATG GACTTACCCA CCAGGGCCTC CTGTATGAAG CAGCCAAGGT GTTTGGTCTT
    CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACATGAAAT TACAGAAGAA
    ATCCCCATGA AGGCTTTGAA CATGAAGACT GTGTATGTTT CTGTATTACC GACGACGGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973605.1
    CDS35..3823
    Translation

    Target ORF information:

    RefSeq Version XM_014118130.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118130.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTCTGGTCCG AATTTAATTG TTTTCAAGAA TGCTTAACGC AATCTAAACA TAAGCCAAAA
    TTTACATTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACCAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAAGA TGTAGCCAAA ATGGGGATTG TAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG
    TGGGTCTTCA AACAGCTCTA TGACAAAGGC CTTGTATATA GAGGCGTGAA AGTCATGCCT
    TTCTCCACCG CGTGCAACAC TCCACTGTCC AACTTCGAGT CTCACCAGAA CTACAAGGAT
    GTTCAAGACC CTTCAGTGTT TATAACTTTC CCTTTGGAGG AAGACGAAAA TGTGTCTTTG
    GTGGCCTGGA CAACCACTCC TTGGACTCTG CCTAGTAACC TGGCCCTGTG TGTTAATCCA
    GAAATGCAGT ATGTGAAGAT TAAAGATGTC ACCAGAGGAA AGTTACTCAT CTTGATGGAA
    GCCAGATTAC TAGCCCTCTA TAAGGTGGAG ACAGACTACG AGATCCTCGA AAGATTTCCC
    GGTAGCTATC TCAAAGGCAA GAAGTACAGG CCGCTGTTTG ACTACTTTGT GCAGCTCAAG
    GAGAATGGTG CTTTCACGGT GCTTGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACCGGA
    GTGGTACACC AGGCTCCTTA CTTCGGGGCT GACGACTATC GGGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCTCC CCCTGTTTGC CCTGTGGATG CCTCAGGCTG TTTCACAGCA
    GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT AATCAGGACC
    CTGAAGGAAA GAGGCCGGCT TCTCATTGCC AGCACCCTCA CTCACAGCTA CCCGTTCTGC
    TGGAGGTCAG ATACTCCCCT GATTTACAAG GCTGTGCCTA GCTGGTTCGT GCGCGTGGAG
    CACATGGTGG ACCAACTCCT GCAGAACAAC GACCTGTGTT ACTGGGTCCC AGAGTTCGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC
    AGATACTGGG GCACGCCCAT CCCCCTGTGG GTCAGCGATG ACTTCGAGGA GGTAGTCTGC
    ATCGGGTCGG TGGCAGAACT GGAAGAACTG ACGGGAGCAA AGGTCTCTGA TCTCCACAGA
    GAGAGTATTG ACCACCTGAC CATCCCTTCT CACTGCGGGA AAGGAGTGTT GCGGCGCATC
    TCCGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT
    CCTTTTGAAA ACAAAAGGGA GTTTGATGAT GCGTTCCCTG CAGACTTCAT CGCTGAAGGC
    ATTGACCAGA CCAGAGGATG GTTTTACACC TTGCTGGTGC TGGCCACTGC TCTCTTTGGA
    CAGCCGCCTT TCAGGAACGT GATTGTGAAC GGGCTGGTCC TGGCAAGTGA TGGTCAGAAG
    ATGAGCAAAC GAAAAAAGAA TTATCCGGAT CCACTTTCAA TCATCCATAA ATACGGTGCC
    GACGCCCTCA GATTATATCT GATCAACTCC CCAGTGGTGA GAGCAGAAAA CCTGCGATTT
    AAGGAGGAGG GTGTGCGTGA TGTCCTGAAG GATGTGCTGC TCCCTTGGTA CAATGCCTAC
    CGTTTCTTCA TCCAGAACGT GCTGAGGCTG CAGAAGGAAG AAGAAATGGA ATTCATCTAC
    AACGAGAACA CAGCTAGAGA AAGCGCTAAC GTCACAGACC GGTGGGTCCT GTCCTGCATG
    CAGTCGCTGG TTGGGTTCTT TGAGACGGAA ATGGCAGCCT ACAGGCTGTA TACCGTGGTA
    CCTCGCCTGG TCAAGTTTGT GGATGTCCTC ACCAACTGGT ACGTCAGGAT GAACCGCAGA
    AGGTTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACGG CCCTGGAGAC CTTGTTCAGT
    GTCCTGCTGT CTCTGTGCAG ACTGATGGCC CCCTATACAC CTTTCCTCAC CGAGTTGATG
    TACCAGAATC TAAAGCTGCT TATCGACCCT GTTTCTGTCC AGGACAAGGA CACACAGAGC
    ATCCACTACC TCATGCTGCC CCACGTTCGA GAGGAGCTGA TTGACAAGAG CACGGAGAGC
    GCAGTGTCTC GGATGCAGTC TGTGATTGAA CTGGGACGAG TGATTCGAGA CCGCAGAACC
    ATTCCAATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAGGATCC GGAAGCTCTT
    CATGAGATCA GGTCTCTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AGAGGTTACC
    TTGTCTACAG ATAAAAACAA GTATGGCATT CGCCTCAGGG CAGAACCTGA TCACATGGTC
    CTAGGGAAGC GTCTGAAGGG AGCCTTCCGG GCAGTCATGG CGGCCATCAA GCAGCTGAGC
    AGTGAGGAGC TGGAGTGCTT CCAGGAGAGT GGGACCATTG TTGTCGAAGG CCATGAGCTG
    CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA AAACAGGAGG GACGACGCAG
    TATGAAGCAC ACTCAGATGC CCAGGCTTTG GTTCTCTTAG ATGTCACCCC CGACCAGTCA
    ATGGTAGATG AAGGAGTGGC TCGGGAGGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCGACTGA TGAAATAACA GTTTATTATA AAGCAAAGTC TGAAGGGAAG
    TATCTGAATA ATGTTATCGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAAGCTCCC
    TTGAAACCGT ATCCAGTTCC AGTGTCTGAT AAAATCATTA TTCAAGAAGA AATGCAGTTA
    AAGGGTTCTG CCCTGGAAAT TACACTCACC AGAGGATCCT CCCTGCCTGG TCCTGTTTGT
    GCATATGTCA ATCTTAACAT TTATGCAAAT GGCAGCGAGC AAGGTGGAGT ATTGCTTCTG
    GAAAACCCAA AAGGCGATAA CAGGTTGGAT GTTTTAAAGC TGAAGAGCAT CATTACTAGT
    ATTTTTGGTG TACGGAATTC TGAGCTGGCT GTCCTCCATG GTGCAGTGGA AATACAAAAC
    CAGACTGATT TACTGAGTAT TAGTGGAAAA ACACTTCGCG TGGCGGCAGG GTCAGCCCCC
    GCTGTAGTGG ACGGCCCCAG CACTGCACTC TGCCGTTACA TCAACCTACA GCTCCTGAAC
    GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACAC TCCTGCTGGA AAACCCGCCT
    GGGCAGAATG GACTTACCCA CCAGGGCCTC CTGTATGAAG CAGCCAAGGT GTTTGGTCTT
    CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACATGAAAT TACAGAAGAA
    ATCCCCATGA AGGCTTTGAA CATGAAGACT GTGTATGTTT CTGTATTACC GACGACGGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01491
    Clone ID Related Accession (Same CDS sequence) XM_014118130.2 , XM_014118130.1 , XM_541332.6 , XM_541332.5
    Accession Version XM_014118130.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product isoleucine--tRNA ligase, cytoplasmic
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTCTGGTCCG AATTTAATTG TTTTCAAGAA TGCTTAACGC AATCTAAACA TAAGCCAAAA
    TTTACATTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACCAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAAGA TGTAGCCAAA ATGGGGATTG TAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG
    TGGGTCTTCA AACAGCTCTA TGACAAAGGC CTTGTATATA GAGGCGTGAA AGTCATGCCT
    TTCTCCACCG CGTGCAACAC TCCACTGTCC AACTTCGAGT CTCACCAGAA CTACAAGGAT
    GTTCAAGACC CTTCAGTGTT TATAACTTTC CCTTTGGAGG AAGACGAAAA TGTGTCTTTG
    GTGGCCTGGA CAACCACTCC TTGGACTCTG CCTAGTAACC TGGCCCTGTG TGTTAATCCA
    GAAATGCAGT ATGTGAAGAT TAAAGATGTC ACCAGAGGAA AGTTACTCAT CTTGATGGAA
    GCCAGATTAC TAGCCCTCTA TAAGGTGGAG ACAGACTACG AGATCCTCGA AAGATTTCCC
    GGTAGCTATC TCAAAGGCAA GAAGTACAGG CCGCTGTTTG ACTACTTTGT GCAGCTCAAG
    GAGAATGGTG CTTTCACGGT GCTTGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACCGGA
    GTGGTACACC AGGCTCCTTA CTTCGGGGCT GACGACTATC GGGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCTCC CCCTGTTTGC CCTGTGGATG CCTCAGGCTG TTTCACAGCA
    GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT AATCAGGACC
    CTGAAGGAAA GAGGCCGGCT TCTCATTGCC AGCACCCTCA CTCACAGCTA CCCGTTCTGC
    TGGAGGTCAG ATACTCCCCT GATTTACAAG GCTGTGCCTA GCTGGTTCGT GCGCGTGGAG
    CACATGGTGG ACCAACTCCT GCAGAACAAC GACCTGTGTT ACTGGGTCCC AGAGTTCGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC
    AGATACTGGG GCACGCCCAT CCCCCTGTGG GTCAGCGATG ACTTCGAGGA GGTAGTCTGC
    ATCGGGTCGG TGGCAGAACT GGAAGAACTG ACGGGAGCAA AGGTCTCTGA TCTCCACAGA
    GAGAGTATTG ACCACCTGAC CATCCCTTCT CACTGCGGGA AAGGAGTGTT GCGGCGCATC
    TCCGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT
    CCTTTTGAAA ACAAAAGGGA GTTTGATGAT GCGTTCCCTG CAGACTTCAT CGCTGAAGGC
    ATTGACCAGA CCAGAGGATG GTTTTACACC TTGCTGGTGC TGGCCACTGC TCTCTTTGGA
    CAGCCGCCTT TCAGGAACGT GATTGTGAAC GGGCTGGTCC TGGCAAGTGA TGGTCAGAAG
    ATGAGCAAAC GAAAAAAGAA TTATCCGGAT CCACTTTCAA TCATCCATAA ATACGGTGCC
    GACGCCCTCA GATTATATCT GATCAACTCC CCAGTGGTGA GAGCAGAAAA CCTGCGATTT
    AAGGAGGAGG GTGTGCGTGA TGTCCTGAAG GATGTGCTGC TCCCTTGGTA CAATGCCTAC
    CGTTTCTTCA TCCAGAACGT GCTGAGGCTG CAGAAGGAAG AAGAAATGGA ATTCATCTAC
    AACGAGAACA CAGCTAGAGA AAGCGCTAAC GTCACAGACC GGTGGGTCCT GTCCTGCATG
    CAGTCGCTGG TTGGGTTCTT TGAGACGGAA ATGGCAGCCT ACAGGCTGTA TACCGTGGTA
    CCTCGCCTGG TCAAGTTTGT GGATGTCCTC ACCAACTGGT ACGTCAGGAT GAACCGCAGA
    AGGTTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACGG CCCTGGAGAC CTTGTTCAGT
    GTCCTGCTGT CTCTGTGCAG ACTGATGGCC CCCTATACAC CTTTCCTCAC CGAGTTGATG
    TACCAGAATC TAAAGCTGCT TATCGACCCT GTTTCTGTCC AGGACAAGGA CACACAGAGC
    ATCCACTACC TCATGCTGCC CCACGTTCGA GAGGAGCTGA TTGACAAGAG CACGGAGAGC
    GCAGTGTCTC GGATGCAGTC TGTGATTGAA CTGGGACGAG TGATTCGAGA CCGCAGAACC
    ATTCCAATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAGGATCC GGAAGCTCTT
    CATGAGATCA GGTCTCTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AGAGGTTACC
    TTGTCTACAG ATAAAAACAA GTATGGCATT CGCCTCAGGG CAGAACCTGA TCACATGGTC
    CTAGGGAAGC GTCTGAAGGG AGCCTTCCGG GCAGTCATGG CGGCCATCAA GCAGCTGAGC
    AGTGAGGAGC TGGAGTGCTT CCAGGAGAGT GGGACCATTG TTGTCGAAGG CCATGAGCTG
    CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA AAACAGGAGG GACGACGCAG
    TATGAAGCAC ACTCAGATGC CCAGGCTTTG GTTCTCTTAG ATGTCACCCC CGACCAGTCA
    ATGGTAGATG AAGGAGTGGC TCGGGAGGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCGACTGA TGAAATAACA GTTTATTATA AAGCAAAGTC TGAAGGGAAG
    TATCTGAATA ATGTTATCGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAAGCTCCC
    TTGAAACCGT ATCCAGTTCC AGTGTCTGAT AAAATCATTA TTCAAGAAGA AATGCAGTTA
    AAGGGTTCTG CCCTGGAAAT TACACTCACC AGAGGATCCT CCCTGCCTGG TCCTGTTTGT
    GCATATGTCA ATCTTAACAT TTATGCAAAT GGCAGCGAGC AAGGTGGAGT ATTGCTTCTG
    GAAAACCCAA AAGGCGATAA CAGGTTGGAT GTTTTAAAGC TGAAGAGCAT CATTACTAGT
    ATTTTTGGTG TACGGAATTC TGAGCTGGCT GTCCTCCATG GTGCAGTGGA AATACAAAAC
    CAGACTGATT TACTGAGTAT TAGTGGAAAA ACACTTCGCG TGGCGGCAGG GTCAGCCCCC
    GCTGTAGTGG ACGGCCCCAG CACTGCACTC TGCCGTTACA TCAACCTACA GCTCCTGAAC
    GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACAC TCCTGCTGGA AAACCCGCCT
    GGGCAGAATG GACTTACCCA CCAGGGCCTC CTGTATGAAG CAGCCAAGGT GTTTGGTCTT
    CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACATGAAAT TACAGAAGAA
    ATCCCCATGA AGGCTTTGAA CATGAAGACT GTGTATGTTT CTGTATTACC GACGACGGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973605.1
    CDS33..3821
    Translation

    Target ORF information:

    RefSeq Version XM_014118130.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118130.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTCTGGTCCG AATTTAATTG TTTTCAAGAA TGCTTAACGC AATCTAAACA TAAGCCAAAA
    TTTACATTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACCAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAAGA TGTAGCCAAA ATGGGGATTG TAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG
    TGGGTCTTCA AACAGCTCTA TGACAAAGGC CTTGTATATA GAGGCGTGAA AGTCATGCCT
    TTCTCCACCG CGTGCAACAC TCCACTGTCC AACTTCGAGT CTCACCAGAA CTACAAGGAT
    GTTCAAGACC CTTCAGTGTT TATAACTTTC CCTTTGGAGG AAGACGAAAA TGTGTCTTTG
    GTGGCCTGGA CAACCACTCC TTGGACTCTG CCTAGTAACC TGGCCCTGTG TGTTAATCCA
    GAAATGCAGT ATGTGAAGAT TAAAGATGTC ACCAGAGGAA AGTTACTCAT CTTGATGGAA
    GCCAGATTAC TAGCCCTCTA TAAGGTGGAG ACAGACTACG AGATCCTCGA AAGATTTCCC
    GGTAGCTATC TCAAAGGCAA GAAGTACAGG CCGCTGTTTG ACTACTTTGT GCAGCTCAAG
    GAGAATGGTG CTTTCACGGT GCTTGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACCGGA
    GTGGTACACC AGGCTCCTTA CTTCGGGGCT GACGACTATC GGGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCTCC CCCTGTTTGC CCTGTGGATG CCTCAGGCTG TTTCACAGCA
    GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT AATCAGGACC
    CTGAAGGAAA GAGGCCGGCT TCTCATTGCC AGCACCCTCA CTCACAGCTA CCCGTTCTGC
    TGGAGGTCAG ATACTCCCCT GATTTACAAG GCTGTGCCTA GCTGGTTCGT GCGCGTGGAG
    CACATGGTGG ACCAACTCCT GCAGAACAAC GACCTGTGTT ACTGGGTCCC AGAGTTCGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC
    AGATACTGGG GCACGCCCAT CCCCCTGTGG GTCAGCGATG ACTTCGAGGA GGTAGTCTGC
    ATCGGGTCGG TGGCAGAACT GGAAGAACTG ACGGGAGCAA AGGTCTCTGA TCTCCACAGA
    GAGAGTATTG ACCACCTGAC CATCCCTTCT CACTGCGGGA AAGGAGTGTT GCGGCGCATC
    TCCGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT
    CCTTTTGAAA ACAAAAGGGA GTTTGATGAT GCGTTCCCTG CAGACTTCAT CGCTGAAGGC
    ATTGACCAGA CCAGAGGATG GTTTTACACC TTGCTGGTGC TGGCCACTGC TCTCTTTGGA
    CAGCCGCCTT TCAGGAACGT GATTGTGAAC GGGCTGGTCC TGGCAAGTGA TGGTCAGAAG
    ATGAGCAAAC GAAAAAAGAA TTATCCGGAT CCACTTTCAA TCATCCATAA ATACGGTGCC
    GACGCCCTCA GATTATATCT GATCAACTCC CCAGTGGTGA GAGCAGAAAA CCTGCGATTT
    AAGGAGGAGG GTGTGCGTGA TGTCCTGAAG GATGTGCTGC TCCCTTGGTA CAATGCCTAC
    CGTTTCTTCA TCCAGAACGT GCTGAGGCTG CAGAAGGAAG AAGAAATGGA ATTCATCTAC
    AACGAGAACA CAGCTAGAGA AAGCGCTAAC GTCACAGACC GGTGGGTCCT GTCCTGCATG
    CAGTCGCTGG TTGGGTTCTT TGAGACGGAA ATGGCAGCCT ACAGGCTGTA TACCGTGGTA
    CCTCGCCTGG TCAAGTTTGT GGATGTCCTC ACCAACTGGT ACGTCAGGAT GAACCGCAGA
    AGGTTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACGG CCCTGGAGAC CTTGTTCAGT
    GTCCTGCTGT CTCTGTGCAG ACTGATGGCC CCCTATACAC CTTTCCTCAC CGAGTTGATG
    TACCAGAATC TAAAGCTGCT TATCGACCCT GTTTCTGTCC AGGACAAGGA CACACAGAGC
    ATCCACTACC TCATGCTGCC CCACGTTCGA GAGGAGCTGA TTGACAAGAG CACGGAGAGC
    GCAGTGTCTC GGATGCAGTC TGTGATTGAA CTGGGACGAG TGATTCGAGA CCGCAGAACC
    ATTCCAATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAGGATCC GGAAGCTCTT
    CATGAGATCA GGTCTCTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AGAGGTTACC
    TTGTCTACAG ATAAAAACAA GTATGGCATT CGCCTCAGGG CAGAACCTGA TCACATGGTC
    CTAGGGAAGC GTCTGAAGGG AGCCTTCCGG GCAGTCATGG CGGCCATCAA GCAGCTGAGC
    AGTGAGGAGC TGGAGTGCTT CCAGGAGAGT GGGACCATTG TTGTCGAAGG CCATGAGCTG
    CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA AAACAGGAGG GACGACGCAG
    TATGAAGCAC ACTCAGATGC CCAGGCTTTG GTTCTCTTAG ATGTCACCCC CGACCAGTCA
    ATGGTAGATG AAGGAGTGGC TCGGGAGGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCGACTGA TGAAATAACA GTTTATTATA AAGCAAAGTC TGAAGGGAAG
    TATCTGAATA ATGTTATCGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAAGCTCCC
    TTGAAACCGT ATCCAGTTCC AGTGTCTGAT AAAATCATTA TTCAAGAAGA AATGCAGTTA
    AAGGGTTCTG CCCTGGAAAT TACACTCACC AGAGGATCCT CCCTGCCTGG TCCTGTTTGT
    GCATATGTCA ATCTTAACAT TTATGCAAAT GGCAGCGAGC AAGGTGGAGT ATTGCTTCTG
    GAAAACCCAA AAGGCGATAA CAGGTTGGAT GTTTTAAAGC TGAAGAGCAT CATTACTAGT
    ATTTTTGGTG TACGGAATTC TGAGCTGGCT GTCCTCCATG GTGCAGTGGA AATACAAAAC
    CAGACTGATT TACTGAGTAT TAGTGGAAAA ACACTTCGCG TGGCGGCAGG GTCAGCCCCC
    GCTGTAGTGG ACGGCCCCAG CACTGCACTC TGCCGTTACA TCAACCTACA GCTCCTGAAC
    GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACAC TCCTGCTGGA AAACCCGCCT
    GGGCAGAATG GACTTACCCA CCAGGGCCTC CTGTATGAAG CAGCCAAGGT GTTTGGTCTT
    CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACATGAAAT TACAGAAGAA
    ATCCCCATGA AGGCTTTGAA CATGAAGACT GTGTATGTTT CTGTATTACC GACGACGGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01491
    Clone ID Related Accession (Same CDS sequence) XM_014118130.2 , XM_014118130.1 , XM_541332.6 , XM_541332.5
    Accession Version XM_541332.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_541332.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTCTGGTCCG AATTTAATTG TTTTCAAGAA TGCTTAACGC AATCTAAACA TAAGCCAAAA
    TTTACATTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACCAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAAGA TGTAGCCAAA ATGGGGATTG TAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG
    TGGGTCTTCA AACAGCTCTA TGACAAAGGC CTTGTATATA GAGGCGTGAA AGTCATGCCT
    TTCTCCACCG CGTGCAACAC TCCACTGTCC AACTTCGAGT CTCACCAGAA CTACAAGGAT
    GTTCAAGACC CTTCAGTGTT TATAACTTTC CCTTTGGAGG AAGACGAAAA TGTGTCTTTG
    GTGGCCTGGA CAACCACTCC TTGGACTCTG CCTAGTAACC TGGCCCTGTG TGTTAATCCA
    GAAATGCAGT ATGTGAAGAT TAAAGATGTC ACCAGAGGAA AGTTACTCAT CTTGATGGAA
    GCCAGATTAC TAGCCCTCTA TAAGGTGGAG ACAGACTACG AGATCCTCGA AAGATTTCCC
    GGTAGCTATC TCAAAGGCAA GAAGTACAGG CCGCTGTTTG ACTACTTTGT GCAGCTCAAG
    GAGAATGGTG CTTTCACGGT GCTTGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACCGGA
    GTGGTACACC AGGCTCCTTA CTTCGGGGCT GACGACTATC GGGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCTCC CCCTGTTTGC CCTGTGGATG CCTCAGGCTG TTTCACAGCA
    GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT AATCAGGACC
    CTGAAGGAAA GAGGCCGGCT TCTCATTGCC AGCACCCTCA CTCACAGCTA CCCGTTCTGC
    TGGAGGTCAG ATACTCCCCT GATTTACAAG GCTGTGCCTA GCTGGTTCGT GCGCGTGGAG
    CACATGGTGG ACCAACTCCT GCAGAACAAC GACCTGTGTT ACTGGGTCCC AGAGTTCGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC
    AGATACTGGG GCACGCCCAT CCCCCTGTGG GTCAGCGATG ACTTCGAGGA GGTAGTCTGC
    ATCGGGTCGG TGGCAGAACT GGAAGAACTG ACGGGAGCAA AGGTCTCTGA TCTCCACAGA
    GAGAGTATTG ACCACCTGAC CATCCCTTCT CACTGCGGGA AAGGAGTGTT GCGGCGCATC
    TCCGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT
    CCTTTTGAAA ACAAAAGGGA GTTTGATGAT GCGTTCCCTG CAGACTTCAT CGCTGAAGGC
    ATTGACCAGA CCAGAGGATG GTTTTACACC TTGCTGGTGC TGGCCACTGC TCTCTTTGGA
    CAGCCGCCTT TCAGGAACGT GATTGTGAAC GGGCTGGTCC TGGCAAGTGA TGGTCAGAAG
    ATGAGCAAAC GAAAAAAGAA TTATCCGGAT CCACTTTCAA TCATCCATAA ATACGGTGCC
    GACGCCCTCA GATTATATCT GATCAACTCC CCAGTGGTGA GAGCAGAAAA CCTGCGATTT
    AAGGAGGAGG GTGTGCGTGA TGTCCTGAAG GATGTGCTGC TCCCTTGGTA CAATGCCTAC
    CGTTTCTTCA TCCAGAACGT GCTGAGGCTG CAGAAGGAAG AAGAAATGGA ATTCATCTAC
    AACGAGAACA CAGCTAGAGA AAGCGCTAAC GTCACAGACC GGTGGGTCCT GTCCTGCATG
    CAGTCGCTGG TTGGGTTCTT TGAGACGGAA ATGGCAGCCT ACAGGCTGTA TACCGTGGTA
    CCTCGCCTGG TCAAGTTTGT GGATGTCCTC ACCAACTGGT ACGTCAGGAT GAACCGCAGA
    AGGTTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACGG CCCTGGAGAC CTTGTTCAGT
    GTCCTGCTGT CTCTGTGCAG ACTGATGGCC CCCTATACAC CTTTCCTCAC CGAGTTGATG
    TACCAGAATC TAAAGCTGCT TATCGACCCT GTTTCTGTCC AGGACAAGGA CACACAGAGC
    ATCCACTACC TCATGCTGCC CCACGTTCGA GAGGAGCTGA TTGACAAGAG CACGGAGAGC
    GCAGTGTCTC GGATGCAGTC TGTGATTGAA CTGGGACGAG TGATTCGAGA CCGCAGAACC
    ATTCCAATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAGGATCC GGAAGCTCTT
    CATGAGATCA GGTCTCTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AGAGGTTACC
    TTGTCTACAG ATAAAAACAA GTATGGCATT CGCCTCAGGG CAGAACCTGA TCACATGGTC
    CTAGGGAAGC GTCTGAAGGG AGCCTTCCGG GCAGTCATGG CGGCCATCAA GCAGCTGAGC
    AGTGAGGAGC TGGAGTGCTT CCAGGAGAGT GGGACCATTG TTGTCGAAGG CCATGAGCTG
    CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA AAACAGGAGG GACGACGCAG
    TATGAAGCAC ACTCAGATGC CCAGGCTTTG GTTCTCTTAG ATGTCACCCC CGACCAGTCA
    ATGGTAGATG AAGGAGTGGC TCGGGAGGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCGACTGA TGAAATAACA GTTTATTATA AAGCAAAGTC TGAAGGGAAG
    TATCTGAATA ATGTTATCGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAAGCTCCC
    TTGAAACCGT ATCCAGTTCC AGTGTCTGAT AAAATCATTA TTCAAGAAGA AATGCAGTTA
    AAGGGTTCTG CCCTGGAAAT TACACTCACC AGAGGATCCT CCCTGCCTGG TCCTGTTTGT
    GCATATGTCA ATCTTAACAT TTATGCAAAT GGCAGCGAGC AAGGTGGAGT ATTGCTTCTG
    GAAAACCCAA AAGGCGATAA CAGGTTGGAT GTTTTAAAGC TGAAGAGCAT CATTACTAGT
    ATTTTTGGTG TACGGAATTC TGAGCTGGCT GTCCTCCATG GTGCAGTGGA AATACAAAAC
    CAGACTGATT TACTGAGTAT TAGTGGAAAA ACACTTCGCG TGGCGGCAGG GTCAGCCCCC
    GCTGTAGTGG ACGGCCCCAG CACTGCACTC TGCCGTTACA TCAACCTACA GCTCCTGAAC
    GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACAC TCCTGCTGGA AAACCCGCCT
    GGGCAGAATG GACTTACCCA CCAGGGCCTC CTGTATGAAG CAGCCAAGGT GTTTGGTCTT
    CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACATGAAAT TACAGAAGAA
    ATCCCCATGA AGGCTTTGAA CATGAAGACT GTGTATGTTT CTGTATTACC GACGACGGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_541332.3
    CDS169..3957
    Translation

    Target ORF information:

    RefSeq Version XM_541332.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_541332.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTCTGGTCCG AATTTAATTG TTTTCAAGAA TGCTTAACGC AATCTAAACA TAAGCCAAAA
    TTTACATTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACCAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAAGA TGTAGCCAAA ATGGGGATTG TAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG
    TGGGTCTTCA AACAGCTCTA TGACAAAGGC CTTGTATATA GAGGCGTGAA AGTCATGCCT
    TTCTCCACCG CGTGCAACAC TCCACTGTCC AACTTCGAGT CTCACCAGAA CTACAAGGAT
    GTTCAAGACC CTTCAGTGTT TATAACTTTC CCTTTGGAGG AAGACGAAAA TGTGTCTTTG
    GTGGCCTGGA CAACCACTCC TTGGACTCTG CCTAGTAACC TGGCCCTGTG TGTTAATCCA
    GAAATGCAGT ATGTGAAGAT TAAAGATGTC ACCAGAGGAA AGTTACTCAT CTTGATGGAA
    GCCAGATTAC TAGCCCTCTA TAAGGTGGAG ACAGACTACG AGATCCTCGA AAGATTTCCC
    GGTAGCTATC TCAAAGGCAA GAAGTACAGG CCGCTGTTTG ACTACTTTGT GCAGCTCAAG
    GAGAATGGTG CTTTCACGGT GCTTGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACCGGA
    GTGGTACACC AGGCTCCTTA CTTCGGGGCT GACGACTATC GGGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCTCC CCCTGTTTGC CCTGTGGATG CCTCAGGCTG TTTCACAGCA
    GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT AATCAGGACC
    CTGAAGGAAA GAGGCCGGCT TCTCATTGCC AGCACCCTCA CTCACAGCTA CCCGTTCTGC
    TGGAGGTCAG ATACTCCCCT GATTTACAAG GCTGTGCCTA GCTGGTTCGT GCGCGTGGAG
    CACATGGTGG ACCAACTCCT GCAGAACAAC GACCTGTGTT ACTGGGTCCC AGAGTTCGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC
    AGATACTGGG GCACGCCCAT CCCCCTGTGG GTCAGCGATG ACTTCGAGGA GGTAGTCTGC
    ATCGGGTCGG TGGCAGAACT GGAAGAACTG ACGGGAGCAA AGGTCTCTGA TCTCCACAGA
    GAGAGTATTG ACCACCTGAC CATCCCTTCT CACTGCGGGA AAGGAGTGTT GCGGCGCATC
    TCCGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT
    CCTTTTGAAA ACAAAAGGGA GTTTGATGAT GCGTTCCCTG CAGACTTCAT CGCTGAAGGC
    ATTGACCAGA CCAGAGGATG GTTTTACACC TTGCTGGTGC TGGCCACTGC TCTCTTTGGA
    CAGCCGCCTT TCAGGAACGT GATTGTGAAC GGGCTGGTCC TGGCAAGTGA TGGTCAGAAG
    ATGAGCAAAC GAAAAAAGAA TTATCCGGAT CCACTTTCAA TCATCCATAA ATACGGTGCC
    GACGCCCTCA GATTATATCT GATCAACTCC CCAGTGGTGA GAGCAGAAAA CCTGCGATTT
    AAGGAGGAGG GTGTGCGTGA TGTCCTGAAG GATGTGCTGC TCCCTTGGTA CAATGCCTAC
    CGTTTCTTCA TCCAGAACGT GCTGAGGCTG CAGAAGGAAG AAGAAATGGA ATTCATCTAC
    AACGAGAACA CAGCTAGAGA AAGCGCTAAC GTCACAGACC GGTGGGTCCT GTCCTGCATG
    CAGTCGCTGG TTGGGTTCTT TGAGACGGAA ATGGCAGCCT ACAGGCTGTA TACCGTGGTA
    CCTCGCCTGG TCAAGTTTGT GGATGTCCTC ACCAACTGGT ACGTCAGGAT GAACCGCAGA
    AGGTTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACGG CCCTGGAGAC CTTGTTCAGT
    GTCCTGCTGT CTCTGTGCAG ACTGATGGCC CCCTATACAC CTTTCCTCAC CGAGTTGATG
    TACCAGAATC TAAAGCTGCT TATCGACCCT GTTTCTGTCC AGGACAAGGA CACACAGAGC
    ATCCACTACC TCATGCTGCC CCACGTTCGA GAGGAGCTGA TTGACAAGAG CACGGAGAGC
    GCAGTGTCTC GGATGCAGTC TGTGATTGAA CTGGGACGAG TGATTCGAGA CCGCAGAACC
    ATTCCAATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAGGATCC GGAAGCTCTT
    CATGAGATCA GGTCTCTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AGAGGTTACC
    TTGTCTACAG ATAAAAACAA GTATGGCATT CGCCTCAGGG CAGAACCTGA TCACATGGTC
    CTAGGGAAGC GTCTGAAGGG AGCCTTCCGG GCAGTCATGG CGGCCATCAA GCAGCTGAGC
    AGTGAGGAGC TGGAGTGCTT CCAGGAGAGT GGGACCATTG TTGTCGAAGG CCATGAGCTG
    CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA AAACAGGAGG GACGACGCAG
    TATGAAGCAC ACTCAGATGC CCAGGCTTTG GTTCTCTTAG ATGTCACCCC CGACCAGTCA
    ATGGTAGATG AAGGAGTGGC TCGGGAGGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCGACTGA TGAAATAACA GTTTATTATA AAGCAAAGTC TGAAGGGAAG
    TATCTGAATA ATGTTATCGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAAGCTCCC
    TTGAAACCGT ATCCAGTTCC AGTGTCTGAT AAAATCATTA TTCAAGAAGA AATGCAGTTA
    AAGGGTTCTG CCCTGGAAAT TACACTCACC AGAGGATCCT CCCTGCCTGG TCCTGTTTGT
    GCATATGTCA ATCTTAACAT TTATGCAAAT GGCAGCGAGC AAGGTGGAGT ATTGCTTCTG
    GAAAACCCAA AAGGCGATAA CAGGTTGGAT GTTTTAAAGC TGAAGAGCAT CATTACTAGT
    ATTTTTGGTG TACGGAATTC TGAGCTGGCT GTCCTCCATG GTGCAGTGGA AATACAAAAC
    CAGACTGATT TACTGAGTAT TAGTGGAAAA ACACTTCGCG TGGCGGCAGG GTCAGCCCCC
    GCTGTAGTGG ACGGCCCCAG CACTGCACTC TGCCGTTACA TCAACCTACA GCTCCTGAAC
    GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACAC TCCTGCTGGA AAACCCGCCT
    GGGCAGAATG GACTTACCCA CCAGGGCCTC CTGTATGAAG CAGCCAAGGT GTTTGGTCTT
    CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACATGAAAT TACAGAAGAA
    ATCCCCATGA AGGCTTTGAA CATGAAGACT GTGTATGTTT CTGTATTACC GACGACGGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01491
    Clone ID Related Accession (Same CDS sequence) XM_014118130.2 , XM_014118130.1 , XM_541332.6 , XM_541332.5
    Accession Version XM_541332.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product isoleucine--tRNA ligase, cytoplasmic
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_541332.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTCTGGTCCG AATTTAATTG TTTTCAAGAA TGCTTAACGC AATCTAAACA TAAGCCAAAA
    TTTACATTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACCAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAAGA TGTAGCCAAA ATGGGGATTG TAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG
    TGGGTCTTCA AACAGCTCTA TGACAAAGGC CTTGTATATA GAGGCGTGAA AGTCATGCCT
    TTCTCCACCG CGTGCAACAC TCCACTGTCC AACTTCGAGT CTCACCAGAA CTACAAGGAT
    GTTCAAGACC CTTCAGTGTT TATAACTTTC CCTTTGGAGG AAGACGAAAA TGTGTCTTTG
    GTGGCCTGGA CAACCACTCC TTGGACTCTG CCTAGTAACC TGGCCCTGTG TGTTAATCCA
    GAAATGCAGT ATGTGAAGAT TAAAGATGTC ACCAGAGGAA AGTTACTCAT CTTGATGGAA
    GCCAGATTAC TAGCCCTCTA TAAGGTGGAG ACAGACTACG AGATCCTCGA AAGATTTCCC
    GGTAGCTATC TCAAAGGCAA GAAGTACAGG CCGCTGTTTG ACTACTTTGT GCAGCTCAAG
    GAGAATGGTG CTTTCACGGT GCTTGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACCGGA
    GTGGTACACC AGGCTCCTTA CTTCGGGGCT GACGACTATC GGGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCTCC CCCTGTTTGC CCTGTGGATG CCTCAGGCTG TTTCACAGCA
    GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT AATCAGGACC
    CTGAAGGAAA GAGGCCGGCT TCTCATTGCC AGCACCCTCA CTCACAGCTA CCCGTTCTGC
    TGGAGGTCAG ATACTCCCCT GATTTACAAG GCTGTGCCTA GCTGGTTCGT GCGCGTGGAG
    CACATGGTGG ACCAACTCCT GCAGAACAAC GACCTGTGTT ACTGGGTCCC AGAGTTCGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC
    AGATACTGGG GCACGCCCAT CCCCCTGTGG GTCAGCGATG ACTTCGAGGA GGTAGTCTGC
    ATCGGGTCGG TGGCAGAACT GGAAGAACTG ACGGGAGCAA AGGTCTCTGA TCTCCACAGA
    GAGAGTATTG ACCACCTGAC CATCCCTTCT CACTGCGGGA AAGGAGTGTT GCGGCGCATC
    TCCGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT
    CCTTTTGAAA ACAAAAGGGA GTTTGATGAT GCGTTCCCTG CAGACTTCAT CGCTGAAGGC
    ATTGACCAGA CCAGAGGATG GTTTTACACC TTGCTGGTGC TGGCCACTGC TCTCTTTGGA
    CAGCCGCCTT TCAGGAACGT GATTGTGAAC GGGCTGGTCC TGGCAAGTGA TGGTCAGAAG
    ATGAGCAAAC GAAAAAAGAA TTATCCGGAT CCACTTTCAA TCATCCATAA ATACGGTGCC
    GACGCCCTCA GATTATATCT GATCAACTCC CCAGTGGTGA GAGCAGAAAA CCTGCGATTT
    AAGGAGGAGG GTGTGCGTGA TGTCCTGAAG GATGTGCTGC TCCCTTGGTA CAATGCCTAC
    CGTTTCTTCA TCCAGAACGT GCTGAGGCTG CAGAAGGAAG AAGAAATGGA ATTCATCTAC
    AACGAGAACA CAGCTAGAGA AAGCGCTAAC GTCACAGACC GGTGGGTCCT GTCCTGCATG
    CAGTCGCTGG TTGGGTTCTT TGAGACGGAA ATGGCAGCCT ACAGGCTGTA TACCGTGGTA
    CCTCGCCTGG TCAAGTTTGT GGATGTCCTC ACCAACTGGT ACGTCAGGAT GAACCGCAGA
    AGGTTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACGG CCCTGGAGAC CTTGTTCAGT
    GTCCTGCTGT CTCTGTGCAG ACTGATGGCC CCCTATACAC CTTTCCTCAC CGAGTTGATG
    TACCAGAATC TAAAGCTGCT TATCGACCCT GTTTCTGTCC AGGACAAGGA CACACAGAGC
    ATCCACTACC TCATGCTGCC CCACGTTCGA GAGGAGCTGA TTGACAAGAG CACGGAGAGC
    GCAGTGTCTC GGATGCAGTC TGTGATTGAA CTGGGACGAG TGATTCGAGA CCGCAGAACC
    ATTCCAATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAGGATCC GGAAGCTCTT
    CATGAGATCA GGTCTCTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AGAGGTTACC
    TTGTCTACAG ATAAAAACAA GTATGGCATT CGCCTCAGGG CAGAACCTGA TCACATGGTC
    CTAGGGAAGC GTCTGAAGGG AGCCTTCCGG GCAGTCATGG CGGCCATCAA GCAGCTGAGC
    AGTGAGGAGC TGGAGTGCTT CCAGGAGAGT GGGACCATTG TTGTCGAAGG CCATGAGCTG
    CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA AAACAGGAGG GACGACGCAG
    TATGAAGCAC ACTCAGATGC CCAGGCTTTG GTTCTCTTAG ATGTCACCCC CGACCAGTCA
    ATGGTAGATG AAGGAGTGGC TCGGGAGGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCGACTGA TGAAATAACA GTTTATTATA AAGCAAAGTC TGAAGGGAAG
    TATCTGAATA ATGTTATCGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAAGCTCCC
    TTGAAACCGT ATCCAGTTCC AGTGTCTGAT AAAATCATTA TTCAAGAAGA AATGCAGTTA
    AAGGGTTCTG CCCTGGAAAT TACACTCACC AGAGGATCCT CCCTGCCTGG TCCTGTTTGT
    GCATATGTCA ATCTTAACAT TTATGCAAAT GGCAGCGAGC AAGGTGGAGT ATTGCTTCTG
    GAAAACCCAA AAGGCGATAA CAGGTTGGAT GTTTTAAAGC TGAAGAGCAT CATTACTAGT
    ATTTTTGGTG TACGGAATTC TGAGCTGGCT GTCCTCCATG GTGCAGTGGA AATACAAAAC
    CAGACTGATT TACTGAGTAT TAGTGGAAAA ACACTTCGCG TGGCGGCAGG GTCAGCCCCC
    GCTGTAGTGG ACGGCCCCAG CACTGCACTC TGCCGTTACA TCAACCTACA GCTCCTGAAC
    GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACAC TCCTGCTGGA AAACCCGCCT
    GGGCAGAATG GACTTACCCA CCAGGGCCTC CTGTATGAAG CAGCCAAGGT GTTTGGTCTT
    CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACATGAAAT TACAGAAGAA
    ATCCCCATGA AGGCTTTGAA CATGAAGACT GTGTATGTTT CTGTATTACC GACGACGGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_541332.3
    CDS119..3907
    Translation

    Target ORF information:

    RefSeq Version XM_541332.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_541332.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAGC AAGTTCCAGA AAACATCAAT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTCTGGTCCG AATTTAATTG TTTTCAAGAA TGCTTAACGC AATCTAAACA TAAGCCAAAA
    TTTACATTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACCAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG
    GGAATCAGAG GACCAGAAGA TGTAGCCAAA ATGGGGATTG TAGAGTATAA CAATCAGTGC
    CGAGCAATTG TGATGAGATA TTCTACTGAG TGGAAGTCCA CTGTTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG
    TGGGTCTTCA AACAGCTCTA TGACAAAGGC CTTGTATATA GAGGCGTGAA AGTCATGCCT
    TTCTCCACCG CGTGCAACAC TCCACTGTCC AACTTCGAGT CTCACCAGAA CTACAAGGAT
    GTTCAAGACC CTTCAGTGTT TATAACTTTC CCTTTGGAGG AAGACGAAAA TGTGTCTTTG
    GTGGCCTGGA CAACCACTCC TTGGACTCTG CCTAGTAACC TGGCCCTGTG TGTTAATCCA
    GAAATGCAGT ATGTGAAGAT TAAAGATGTC ACCAGAGGAA AGTTACTCAT CTTGATGGAA
    GCCAGATTAC TAGCCCTCTA TAAGGTGGAG ACAGACTACG AGATCCTCGA AAGATTTCCC
    GGTAGCTATC TCAAAGGCAA GAAGTACAGG CCGCTGTTTG ACTACTTTGT GCAGCTCAAG
    GAGAATGGTG CTTTCACGGT GCTTGCTGAC AGCTACGTGC GGGAGGAGGA GGGCACCGGA
    GTGGTACACC AGGCTCCTTA CTTCGGGGCT GACGACTATC GGGTCTGTAT GGACTTCAAC
    ATCATTCAGA AAGACTCTCC CCCTGTTTGC CCTGTGGATG CCTCAGGCTG TTTCACAGCA
    GAAGTGACAG ATTTCATGGG ACAGTATGTG AAGGATGCTG ACAAAAACAT AATCAGGACC
    CTGAAGGAAA GAGGCCGGCT TCTCATTGCC AGCACCCTCA CTCACAGCTA CCCGTTCTGC
    TGGAGGTCAG ATACTCCCCT GATTTACAAG GCTGTGCCTA GCTGGTTCGT GCGCGTGGAG
    CACATGGTGG ACCAACTCCT GCAGAACAAC GACCTGTGTT ACTGGGTCCC AGAGTTCGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC
    AGATACTGGG GCACGCCCAT CCCCCTGTGG GTCAGCGATG ACTTCGAGGA GGTAGTCTGC
    ATCGGGTCGG TGGCAGAACT GGAAGAACTG ACGGGAGCAA AGGTCTCTGA TCTCCACAGA
    GAGAGTATTG ACCACCTGAC CATCCCTTCT CACTGCGGGA AAGGAGTGTT GCGGCGCATC
    TCCGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CCTATGCTCA AGTTCATTAT
    CCTTTTGAAA ACAAAAGGGA GTTTGATGAT GCGTTCCCTG CAGACTTCAT CGCTGAAGGC
    ATTGACCAGA CCAGAGGATG GTTTTACACC TTGCTGGTGC TGGCCACTGC TCTCTTTGGA
    CAGCCGCCTT TCAGGAACGT GATTGTGAAC GGGCTGGTCC TGGCAAGTGA TGGTCAGAAG
    ATGAGCAAAC GAAAAAAGAA TTATCCGGAT CCACTTTCAA TCATCCATAA ATACGGTGCC
    GACGCCCTCA GATTATATCT GATCAACTCC CCAGTGGTGA GAGCAGAAAA CCTGCGATTT
    AAGGAGGAGG GTGTGCGTGA TGTCCTGAAG GATGTGCTGC TCCCTTGGTA CAATGCCTAC
    CGTTTCTTCA TCCAGAACGT GCTGAGGCTG CAGAAGGAAG AAGAAATGGA ATTCATCTAC
    AACGAGAACA CAGCTAGAGA AAGCGCTAAC GTCACAGACC GGTGGGTCCT GTCCTGCATG
    CAGTCGCTGG TTGGGTTCTT TGAGACGGAA ATGGCAGCCT ACAGGCTGTA TACCGTGGTA
    CCTCGCCTGG TCAAGTTTGT GGATGTCCTC ACCAACTGGT ACGTCAGGAT GAACCGCAGA
    AGGTTGAAGG GTGAAAACGG GGTGGAGGAC TGTGTTACGG CCCTGGAGAC CTTGTTCAGT
    GTCCTGCTGT CTCTGTGCAG ACTGATGGCC CCCTATACAC CTTTCCTCAC CGAGTTGATG
    TACCAGAATC TAAAGCTGCT TATCGACCCT GTTTCTGTCC AGGACAAGGA CACACAGAGC
    ATCCACTACC TCATGCTGCC CCACGTTCGA GAGGAGCTGA TTGACAAGAG CACGGAGAGC
    GCAGTGTCTC GGATGCAGTC TGTGATTGAA CTGGGACGAG TGATTCGAGA CCGCAGAACC
    ATTCCAATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAGGATCC GGAAGCTCTT
    CATGAGATCA GGTCTCTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AGAGGTTACC
    TTGTCTACAG ATAAAAACAA GTATGGCATT CGCCTCAGGG CAGAACCTGA TCACATGGTC
    CTAGGGAAGC GTCTGAAGGG AGCCTTCCGG GCAGTCATGG CGGCCATCAA GCAGCTGAGC
    AGTGAGGAGC TGGAGTGCTT CCAGGAGAGT GGGACCATTG TTGTCGAAGG CCATGAGCTG
    CATGAAGAGG ACATCCGCCT CATGTACACC TTTGATCAGA AAACAGGAGG GACGACGCAG
    TATGAAGCAC ACTCAGATGC CCAGGCTTTG GTTCTCTTAG ATGTCACCCC CGACCAGTCA
    ATGGTAGATG AAGGAGTGGC TCGGGAGGTC ATCAATCGCA TCCAGAAACT TCGCAAAAAG
    TGCAATCTGG TTCCGACTGA TGAAATAACA GTTTATTATA AAGCAAAGTC TGAAGGGAAG
    TATCTGAATA ATGTTATCGA AAGCCACATG GAGTTCATAT TTGCCACCAT AAAAGCTCCC
    TTGAAACCGT ATCCAGTTCC AGTGTCTGAT AAAATCATTA TTCAAGAAGA AATGCAGTTA
    AAGGGTTCTG CCCTGGAAAT TACACTCACC AGAGGATCCT CCCTGCCTGG TCCTGTTTGT
    GCATATGTCA ATCTTAACAT TTATGCAAAT GGCAGCGAGC AAGGTGGAGT ATTGCTTCTG
    GAAAACCCAA AAGGCGATAA CAGGTTGGAT GTTTTAAAGC TGAAGAGCAT CATTACTAGT
    ATTTTTGGTG TACGGAATTC TGAGCTGGCT GTCCTCCATG GTGCAGTGGA AATACAAAAC
    CAGACTGATT TACTGAGTAT TAGTGGAAAA ACACTTCGCG TGGCGGCAGG GTCAGCCCCC
    GCTGTAGTGG ACGGCCCCAG CACTGCACTC TGCCGTTACA TCAACCTACA GCTCCTGAAC
    GCAGGCCCGC AAGAGTGTGT GAAGGGAACA GTGGGCACAC TCCTGCTGGA AAACCCGCCT
    GGGCAGAATG GACTTACCCA CCAGGGCCTC CTGTATGAAG CAGCCAAGGT GTTTGGTCTT
    CGCAGCAGGA AGCTCAAGCT GTTTCTGAAC GAGACTCAGA CACATGAAAT TACAGAAGAA
    ATCCCCATGA AGGCTTTGAA CATGAAGACT GTGTATGTTT CTGTATTACC GACGACGGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.