PTCH1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following PTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe01271 XM_003638871.3
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Canis lupus familiaris patched 1 (PTCH1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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OCe01271 XM_003638871.2 Canis lupus familiaris patched 1 (PTCH1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe01271
    Clone ID Related Accession (Same CDS sequence) XM_003638871.3 , XM_003638871.2
    Accession Version XM_003638871.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LOW QUALITY PROTEIN: protein patched homolog 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003638871.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCGGAGCCC CAGGACCGCG GCGGTGGCGG CGGCAGCGGG 
    GCCCCCGGCC GGCCGGCCGG CGGCGGGAGA CGCAGACGGG CCGGGGGGCT GCGCCGCAGC
    GCCGCGCTGG ACCGGGACTA TCTGCACCGG CCCAGCTACT GCGACGCCGC CTTCGCTCTG
    GAGCAGATTT CCAAGGGGAA AGCTACTGGC CGGAAAGCGC CGCTGTGGCT GAGAGCGAAG
    TTTCAGAGAC TCTTATTTAA ACTGGGTTGT TACATTCAAA AAAACTGCGG CAAGTTCTTG
    GTTGTGGGCC TCCTCATATT TGGGGCCTTC GCTGTGGGAT TAAAGGCAGC TAATCTCGAG
    ACCAACGTGG AGGAGCTGTG GGTGGAAGTT GGTGGACGAG TAAGTCGTGA ATTAAATTAT
    ACTCGCCAGA AGATTGGAGA AGAGGCTATG TTTAATCCTC AACTCATGAT TCAGACCCCG
    AAAGAAGAAG GTGCTAACGT CCTGACCACA GAAGCTCTCC GACAACACCT GGACTCAGCG
    CTCCAGGCCA GCCGGGTCCA TGTGTATATG TATAACAGGC AGTGGAAATT GGAACATTTG
    TGTTACAAAT CTGGAGAACT TATCACAGAA ACAGGTTACA TGGATCAGAT AATCGAATAT
    CTTTACCCTT GTTTAATTAT TACACCTTTG GACTGCTTCT GGGAAGGGGC AAAGTTACAA
    TCTGGGACAG CATACCTACT AGGTAAGCCT CCTTTGCAGT GGACAAACTT TGACCCTTTG
    GAATTCCTGG AAGAGTTAAA GAAAATAAAC TATCAAGTGG ACAGCTGGGA GGAAATGCTG
    AATAAAGCTG AAGTTGGTCA TGGTTACATG GACCGGCCCT GCCTCAACCC GGCTGATCCG
    GACTGCCCCG CCACAGCCCC CAACAAAAAT GCAACCAAAC CTCTTGATGT GGCCCTTGTT
    TTGAATGGTG GATGTCATGG ATTATCCAGA AAGTACATGC ACTGGCAGGA GGAACTGATT
    GTGGGTGGCA CAGTCAAGAA CAGCACCGGG AAGCTTGTCA GCGCCCACGC CCTGCAAACC
    ATGTTTCAGT TAATGACTCC CAAGCAAATG TACGAACACT TCAAGGGGTA TGAGTATGTC
    TCACACATTA ACTGGAATGA GGACAAGGCA GCAGCCATCC TGGAGGCTTG GCAGAGGACA
    TATGTGGAGG TGGTTCATCA AAGTGTCGCT CCAAACTCCA CTCAGAAAGT GCTTTCCTTC
    ACCACCACAA CCCTGGATGA CATCCTGAAG TCCTTCTCTG ATGTCAGCGT CATCCGAGTG
    GCTAGTGGCT ACCTCCTGAT GCTTGCCTAT GCCTGTTTAA CCATGCTGCG CTGGGATTGC
    TCCAAGTCCC AGGGTGCCGT GGGGCTGGCT GGCGTCCTGT TGGTTGCACT GTCAGTGGCT
    GCAGGATTGG GCCTGTGCTC ATTGATCGGG ATTTCCTTTA ACGCTGCAAC AACTCAGGTT
    TTGCCATTTC TCGCTCTTGG TGTTGGTGTG GATGATGTTT TCCTTCTGGC ACATGCATTT
    AGTGAAACAG GACAGAATAA AAGAATCCCT TTTGAGGATA GGACTGGGGA GTGCCTGAAG
    CGCACAGGAG CCAGTGTGGC GCTCACCTCC ATCAGCAATG TCACAGCCTT CTTCATGGCT
    GCTCTGATCC CTATTCCCGC TCTGCGAGCC TTCTCCCTTC AGGCGGCTGT AGTAGTGGTA
    TTCAATTTTG CTATGGTTCT GCTCATTTTT CCTGCAATTC TCAGCATGGA TTTGTATCGA
    CGTGAGGACA GGAGATTGGA TATTTTCTGC TGTTTTACAA GCCCCTGCGT CAGCAGAGTG
    ATTCAGGTTG AACCTCAGGC CTACACGGAG ACTCACGATA ACACTCGCTA CAGTCCCCCG
    CCCCCCTACA GCAGCCACAG CTTCACCCAC GAAACCCAGA TCACCATGCA GTCCACAGTA
    CAGCTCCGCA CAGAGTATGA CCCCCACACG CACGTGTACT ACACCACCGC CGAGCCACGC
    TCTGAGATCT CCGTGCAGCC CGTCACCATG ACCCAGGATA CCCTCAGCTG CCAGAGCCCT
    GAGAGCACCA GCTCCACGAG GGACCTGCTC TCCCAGTTCT CTGACTCCAG CCTCCACTGC
    CTTGAGCCCC CTTGCACCAA GTGGACACTT TCATCTTTTG CCGAGAAACA CTATGCTCCT
    TTCCTCTTGA AACCAAAAGC CAAGGTCGTG GTCATCTTCC TTTTCCTGGG CTTGCTGGGG
    GTCAGCCTTT ATGGGACCAC CCGAGTGCGC GATGGGCTGG ACCTCACAGA CATTGTGCCC
    CGGGAAACCA GAGAATATGA TTTTATTGCT GCACAGTTCA AATACTTCTC TTTCTACAAC
    ATGTATATAG TCACCCAGAA AGCAGACTAC CCAAATATCC AGCACTTACT TTACGACCTT
    CACAAGAGTT TCAGCAATGT GAAGTATGTC ATGTTGGAAG AAAATAAACA GCTTCCCAAA
    ATGTGGCTGC ATTACTTCCG AGACTGGTTA CAAGGACTTC AGGACGCATT TGACAGTGAC
    TGGGAAACTG GAAAANNTCA TGCCAACAAA TTCAAAAAAG GATCAGATGA TGGGGTCCTT
    GCCTACAAAC TCCTGGTGCA GACCGGCAGC CGCGATAAAC CCATCGACAT CAGTCAGTTG
    ACCAAGCAGC GCCTGGTGGA TGCACATGGC ATCATCAACC CCAGTGCATT CTACATCTAC
    CTGACTGCCT GGGTGAGCAA TGACCCTGTG GCTTATGCCG CCTCCCAGGC CAACATCCGG
    CCTCACCGTC CTGAGTGGGT GCATGACAAA GCTGACTACA TGCCCGAAAC CAGGCTCAGA
    ATCCCAGCAG CAGAGCCCAT CGAATACGCT CAGTTCCCTT TCTACCTCAA TGGCTTGCGG
    GACACATCTG ACTTCGTGGA AGCTATTGAA AAGGTCCGAA GCATCTGCAA CAACTACACC
    AGCCTGGGGC TGTCCAGCTA CCCCAACGGC TACCCCTTCC TCTTCTGGGA GCAGTACATT
    GGCCTCCGCC ACTGGCTTCT GTTGTCTGTC AGCGTGGTGC TGGCCTGCAC GTTCCTCGTA
    TGCGCCGTCT TCCTCCTGAA CCCCTGGACG GCCGGGATCA TTGTGACGGT GCTGGCTTTG
    ATGACAGTCG AGCTCTTTGG CATGATGGGC CTCATTGGAA TCAAGCTGAG TGCTGTGCCA
    GTGGTCATCC TGATCGCTTC TGTCGGCATA GGAGTGGAGT TCACCGTTCA CGTTGCTCTG
    GCTTTCCTGA CAGCCATTGG TGATAAGAAC CGCAGAGCCG TGCTGGCGCT GGAGCACATG
    TTCGCGCCCG TCCTGGATGG CGCCGTGTCC ACTCTGCTGG GAGTGCTGAT GCTGGCGGGC
    TCGGAGTTTG ATTTCATTGT CAGGTATTTC TTTGCTGTTC TGGCGATCCT AACAATCCTG
    GGTGTTCTCA ACGGGCTGGT TTTGCTGCCA GTCCTTCTGT CCTTCTTCGG ACCGTATCCT
    GAGGTATCTC CAGCCAATGG GCTGAACCGA CTGCCCACCC CTTCCCCTGA GCCACCCCCC
    AGTGTGGTCC GGTTTGCCGT GCCACCCAGC CATGCAAACA ATGGGTCTGA TTCCTCTGAC
    TCGGAGTACA GTTCTCAGAC TACAGTGTCG GGCATCAGTG AAGAGCTTCG GCACTACGAG
    GCCCAGCAGG GCACCAGGGG CCCTGCCCAC CAGGTGATCG TGGAAGCCAC AGAAAACCCC
    GTCTTTGCCC GCTCCACTGT GGTCCATCCC GAAGCGAGAC ATCACCCACC CTCAAACCCT
    CGACAGCAGC CCCCCCTGGA CTCAGAGCCC CTTCCTCCTG GGCGACCAGG CCAGCAGCCC
    CGGAGGGAAC CCCCCAGAGA AGGCTTGCGG CCGCCCCCCT ACAGACCGCG CAGAGACGCT
    TTTGAAATTT CTACTGACGG GCATCCTGGC CCTGGCAGTA GGGACCGTGC GGGCCCCCGG
    GGAGCCCGTT CTAACAACCC CCGGCACCCA GTGTTCACTG CCATGGGCAG CTCCGCGCCC
    AGCTACTGCC AGCCCATCAC CACTGTGACG GCCTCGGCGT CCGTGACTGT TGCTGTGCAC
    CCCCCGCCTG CGCCTGGGCC TGGCTCTGCT TGGAACCCCA GAGGAGGACT CTGTCCGGGC
    TACGGGGACT GCCCGGAGAC TGACCACAGG CTATTTGAGG ACCCCCACGT GCCTTTTAAC
    GTCCGGTGCG AGAGGAGGGA TTCCAAGGTG GAAGTCATAG AGCTGCAGGA TGTGGAATGT
    GAGGAGAGAC CCCGGGGCGG TAGCTCCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003638919.1
    CDS16..4368
    Translation

    Target ORF information:

    RefSeq Version XM_003638871.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris patched 1 (PTCH1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003638871.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCGGAGCCC CAGGACCGCG GCGGTGGCGG CGGCAGCGGG 
    GCCCCCGGCC GGCCGGCCGG CGGCGGGAGA CGCAGACGGG CCGGGGGGCT GCGCCGCAGC
    GCCGCGCTGG ACCGGGACTA TCTGCACCGG CCCAGCTACT GCGACGCCGC CTTCGCTCTG
    GAGCAGATTT CCAAGGGGAA AGCTACTGGC CGGAAAGCGC CGCTGTGGCT GAGAGCGAAG
    TTTCAGAGAC TCTTATTTAA ACTGGGTTGT TACATTCAAA AAAACTGCGG CAAGTTCTTG
    GTTGTGGGCC TCCTCATATT TGGGGCCTTC GCTGTGGGAT TAAAGGCAGC TAATCTCGAG
    ACCAACGTGG AGGAGCTGTG GGTGGAAGTT GGTGGACGAG TAAGTCGTGA ATTAAATTAT
    ACTCGCCAGA AGATTGGAGA AGAGGCTATG TTTAATCCTC AACTCATGAT TCAGACCCCG
    AAAGAAGAAG GTGCTAACGT CCTGACCACA GAAGCTCTCC GACAACACCT GGACTCAGCG
    CTCCAGGCCA GCCGGGTCCA TGTGTATATG TATAACAGGC AGTGGAAATT GGAACATTTG
    TGTTACAAAT CTGGAGAACT TATCACAGAA ACAGGTTACA TGGATCAGAT AATCGAATAT
    CTTTACCCTT GTTTAATTAT TACACCTTTG GACTGCTTCT GGGAAGGGGC AAAGTTACAA
    TCTGGGACAG CATACCTACT AGGTAAGCCT CCTTTGCAGT GGACAAACTT TGACCCTTTG
    GAATTCCTGG AAGAGTTAAA GAAAATAAAC TATCAAGTGG ACAGCTGGGA GGAAATGCTG
    AATAAAGCTG AAGTTGGTCA TGGTTACATG GACCGGCCCT GCCTCAACCC GGCTGATCCG
    GACTGCCCCG CCACAGCCCC CAACAAAAAT GCAACCAAAC CTCTTGATGT GGCCCTTGTT
    TTGAATGGTG GATGTCATGG ATTATCCAGA AAGTACATGC ACTGGCAGGA GGAACTGATT
    GTGGGTGGCA CAGTCAAGAA CAGCACCGGG AAGCTTGTCA GCGCCCACGC CCTGCAAACC
    ATGTTTCAGT TAATGACTCC CAAGCAAATG TACGAACACT TCAAGGGGTA TGAGTATGTC
    TCACACATTA ACTGGAATGA GGACAAGGCA GCAGCCATCC TGGAGGCTTG GCAGAGGACA
    TATGTGGAGG TGGTTCATCA AAGTGTCGCT CCAAACTCCA CTCAGAAAGT GCTTTCCTTC
    ACCACCACAA CCCTGGATGA CATCCTGAAG TCCTTCTCTG ATGTCAGCGT CATCCGAGTG
    GCTAGTGGCT ACCTCCTGAT GCTTGCCTAT GCCTGTTTAA CCATGCTGCG CTGGGATTGC
    TCCAAGTCCC AGGGTGCCGT GGGGCTGGCT GGCGTCCTGT TGGTTGCACT GTCAGTGGCT
    GCAGGATTGG GCCTGTGCTC ATTGATCGGG ATTTCCTTTA ACGCTGCAAC AACTCAGGTT
    TTGCCATTTC TCGCTCTTGG TGTTGGTGTG GATGATGTTT TCCTTCTGGC ACATGCATTT
    AGTGAAACAG GACAGAATAA AAGAATCCCT TTTGAGGATA GGACTGGGGA GTGCCTGAAG
    CGCACAGGAG CCAGTGTGGC GCTCACCTCC ATCAGCAATG TCACAGCCTT CTTCATGGCT
    GCTCTGATCC CTATTCCCGC TCTGCGAGCC TTCTCCCTTC AGGCGGCTGT AGTAGTGGTA
    TTCAATTTTG CTATGGTTCT GCTCATTTTT CCTGCAATTC TCAGCATGGA TTTGTATCGA
    CGTGAGGACA GGAGATTGGA TATTTTCTGC TGTTTTACAA GCCCCTGCGT CAGCAGAGTG
    ATTCAGGTTG AACCTCAGGC CTACACGGAG ACTCACGATA ACACTCGCTA CAGTCCCCCG
    CCCCCCTACA GCAGCCACAG CTTCACCCAC GAAACCCAGA TCACCATGCA GTCCACAGTA
    CAGCTCCGCA CAGAGTATGA CCCCCACACG CACGTGTACT ACACCACCGC CGAGCCACGC
    TCTGAGATCT CCGTGCAGCC CGTCACCATG ACCCAGGATA CCCTCAGCTG CCAGAGCCCT
    GAGAGCACCA GCTCCACGAG GGACCTGCTC TCCCAGTTCT CTGACTCCAG CCTCCACTGC
    CTTGAGCCCC CTTGCACCAA GTGGACACTT TCATCTTTTG CCGAGAAACA CTATGCTCCT
    TTCCTCTTGA AACCAAAAGC CAAGGTCGTG GTCATCTTCC TTTTCCTGGG CTTGCTGGGG
    GTCAGCCTTT ATGGGACCAC CCGAGTGCGC GATGGGCTGG ACCTCACAGA CATTGTGCCC
    CGGGAAACCA GAGAATATGA TTTTATTGCT GCACAGTTCA AATACTTCTC TTTCTACAAC
    ATGTATATAG TCACCCAGAA AGCAGACTAC CCAAATATCC AGCACTTACT TTACGACCTT
    CACAAGAGTT TCAGCAATGT GAAGTATGTC ATGTTGGAAG AAAATAAACA GCTTCCCAAA
    ATGTGGCTGC ATTACTTCCG AGACTGGTTA CAAGGACTTC AGGACGCATT TGACAGTGAC
    TGGGAAACTG GAAAANNTCA TGCCAACAAA TTCAAAAAAG GATCAGATGA TGGGGTCCTT
    GCCTACAAAC TCCTGGTGCA GACCGGCAGC CGCGATAAAC CCATCGACAT CAGTCAGTTG
    ACCAAGCAGC GCCTGGTGGA TGCACATGGC ATCATCAACC CCAGTGCATT CTACATCTAC
    CTGACTGCCT GGGTGAGCAA TGACCCTGTG GCTTATGCCG CCTCCCAGGC CAACATCCGG
    CCTCACCGTC CTGAGTGGGT GCATGACAAA GCTGACTACA TGCCCGAAAC CAGGCTCAGA
    ATCCCAGCAG CAGAGCCCAT CGAATACGCT CAGTTCCCTT TCTACCTCAA TGGCTTGCGG
    GACACATCTG ACTTCGTGGA AGCTATTGAA AAGGTCCGAA GCATCTGCAA CAACTACACC
    AGCCTGGGGC TGTCCAGCTA CCCCAACGGC TACCCCTTCC TCTTCTGGGA GCAGTACATT
    GGCCTCCGCC ACTGGCTTCT GTTGTCTGTC AGCGTGGTGC TGGCCTGCAC GTTCCTCGTA
    TGCGCCGTCT TCCTCCTGAA CCCCTGGACG GCCGGGATCA TTGTGACGGT GCTGGCTTTG
    ATGACAGTCG AGCTCTTTGG CATGATGGGC CTCATTGGAA TCAAGCTGAG TGCTGTGCCA
    GTGGTCATCC TGATCGCTTC TGTCGGCATA GGAGTGGAGT TCACCGTTCA CGTTGCTCTG
    GCTTTCCTGA CAGCCATTGG TGATAAGAAC CGCAGAGCCG TGCTGGCGCT GGAGCACATG
    TTCGCGCCCG TCCTGGATGG CGCCGTGTCC ACTCTGCTGG GAGTGCTGAT GCTGGCGGGC
    TCGGAGTTTG ATTTCATTGT CAGGTATTTC TTTGCTGTTC TGGCGATCCT AACAATCCTG
    GGTGTTCTCA ACGGGCTGGT TTTGCTGCCA GTCCTTCTGT CCTTCTTCGG ACCGTATCCT
    GAGGTATCTC CAGCCAATGG GCTGAACCGA CTGCCCACCC CTTCCCCTGA GCCACCCCCC
    AGTGTGGTCC GGTTTGCCGT GCCACCCAGC CATGCAAACA ATGGGTCTGA TTCCTCTGAC
    TCGGAGTACA GTTCTCAGAC TACAGTGTCG GGCATCAGTG AAGAGCTTCG GCACTACGAG
    GCCCAGCAGG GCACCAGGGG CCCTGCCCAC CAGGTGATCG TGGAAGCCAC AGAAAACCCC
    GTCTTTGCCC GCTCCACTGT GGTCCATCCC GAAGCGAGAC ATCACCCACC CTCAAACCCT
    CGACAGCAGC CCCCCCTGGA CTCAGAGCCC CTTCCTCCTG GGCGACCAGG CCAGCAGCCC
    CGGAGGGAAC CCCCCAGAGA AGGCTTGCGG CCGCCCCCCT ACAGACCGCG CAGAGACGCT
    TTTGAAATTT CTACTGACGG GCATCCTGGC CCTGGCAGTA GGGACCGTGC GGGCCCCCGG
    GGAGCCCGTT CTAACAACCC CCGGCACCCA GTGTTCACTG CCATGGGCAG CTCCGCGCCC
    AGCTACTGCC AGCCCATCAC CACTGTGACG GCCTCGGCGT CCGTGACTGT TGCTGTGCAC
    CCCCCGCCTG CGCCTGGGCC TGGCTCTGCT TGGAACCCCA GAGGAGGACT CTGTCCGGGC
    TACGGGGACT GCCCGGAGAC TGACCACAGG CTATTTGAGG ACCCCCACGT GCCTTTTAAC
    GTCCGGTGCG AGAGGAGGGA TTCCAAGGTG GAAGTCATAG AGCTGCAGGA TGTGGAATGT
    GAGGAGAGAC CCCGGGGCGG TAGCTCCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01271
    Clone ID Related Accession (Same CDS sequence) XM_003638871.3 , XM_003638871.2
    Accession Version XM_003638871.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LOW QUALITY PROTEIN: protein patched homolog 1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_003638871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCGGAGCCC CAGGACCGCG GCGGTGGCGG CGGCAGCGGG 
    GCCCCCGGCC GGCCGGCCGG CGGCGGGAGA CGCAGACGGG CCGGGGGGCT GCGCCGCAGC
    GCCGCGCTGG ACCGGGACTA TCTGCACCGG CCCAGCTACT GCGACGCCGC CTTCGCTCTG
    GAGCAGATTT CCAAGGGGAA AGCTACTGGC CGGAAAGCGC CGCTGTGGCT GAGAGCGAAG
    TTTCAGAGAC TCTTATTTAA ACTGGGTTGT TACATTCAAA AAAACTGCGG CAAGTTCTTG
    GTTGTGGGCC TCCTCATATT TGGGGCCTTC GCTGTGGGAT TAAAGGCAGC TAATCTCGAG
    ACCAACGTGG AGGAGCTGTG GGTGGAAGTT GGTGGACGAG TAAGTCGTGA ATTAAATTAT
    ACTCGCCAGA AGATTGGAGA AGAGGCTATG TTTAATCCTC AACTCATGAT TCAGACCCCG
    AAAGAAGAAG GTGCTAACGT CCTGACCACA GAAGCTCTCC GACAACACCT GGACTCAGCG
    CTCCAGGCCA GCCGGGTCCA TGTGTATATG TATAACAGGC AGTGGAAATT GGAACATTTG
    TGTTACAAAT CTGGAGAACT TATCACAGAA ACAGGTTACA TGGATCAGAT AATCGAATAT
    CTTTACCCTT GTTTAATTAT TACACCTTTG GACTGCTTCT GGGAAGGGGC AAAGTTACAA
    TCTGGGACAG CATACCTACT AGGTAAGCCT CCTTTGCAGT GGACAAACTT TGACCCTTTG
    GAATTCCTGG AAGAGTTAAA GAAAATAAAC TATCAAGTGG ACAGCTGGGA GGAAATGCTG
    AATAAAGCTG AAGTTGGTCA TGGTTACATG GACCGGCCCT GCCTCAACCC GGCTGATCCG
    GACTGCCCCG CCACAGCCCC CAACAAAAAT GCAACCAAAC CTCTTGATGT GGCCCTTGTT
    TTGAATGGTG GATGTCATGG ATTATCCAGA AAGTACATGC ACTGGCAGGA GGAACTGATT
    GTGGGTGGCA CAGTCAAGAA CAGCACCGGG AAGCTTGTCA GCGCCCACGC CCTGCAAACC
    ATGTTTCAGT TAATGACTCC CAAGCAAATG TACGAACACT TCAAGGGGTA TGAGTATGTC
    TCACACATTA ACTGGAATGA GGACAAGGCA GCAGCCATCC TGGAGGCTTG GCAGAGGACA
    TATGTGGAGG TGGTTCATCA AAGTGTCGCT CCAAACTCCA CTCAGAAAGT GCTTTCCTTC
    ACCACCACAA CCCTGGATGA CATCCTGAAG TCCTTCTCTG ATGTCAGCGT CATCCGAGTG
    GCTAGTGGCT ACCTCCTGAT GCTTGCCTAT GCCTGTTTAA CCATGCTGCG CTGGGATTGC
    TCCAAGTCCC AGGGTGCCGT GGGGCTGGCT GGCGTCCTGT TGGTTGCACT GTCAGTGGCT
    GCAGGATTGG GCCTGTGCTC ATTGATCGGG ATTTCCTTTA ACGCTGCAAC AACTCAGGTT
    TTGCCATTTC TCGCTCTTGG TGTTGGTGTG GATGATGTTT TCCTTCTGGC ACATGCATTT
    AGTGAAACAG GACAGAATAA AAGAATCCCT TTTGAGGATA GGACTGGGGA GTGCCTGAAG
    CGCACAGGAG CCAGTGTGGC GCTCACCTCC ATCAGCAATG TCACAGCCTT CTTCATGGCT
    GCTCTGATCC CTATTCCCGC TCTGCGAGCC TTCTCCCTTC AGGCGGCTGT AGTAGTGGTA
    TTCAATTTTG CTATGGTTCT GCTCATTTTT CCTGCAATTC TCAGCATGGA TTTGTATCGA
    CGTGAGGACA GGAGATTGGA TATTTTCTGC TGTTTTACAA GCCCCTGCGT CAGCAGAGTG
    ATTCAGGTTG AACCTCAGGC CTACACGGAG ACTCACGATA ACACTCGCTA CAGTCCCCCG
    CCCCCCTACA GCAGCCACAG CTTCACCCAC GAAACCCAGA TCACCATGCA GTCCACAGTA
    CAGCTCCGCA CAGAGTATGA CCCCCACACG CACGTGTACT ACACCACCGC CGAGCCACGC
    TCTGAGATCT CCGTGCAGCC CGTCACCATG ACCCAGGATA CCCTCAGCTG CCAGAGCCCT
    GAGAGCACCA GCTCCACGAG GGACCTGCTC TCCCAGTTCT CTGACTCCAG CCTCCACTGC
    CTTGAGCCCC CTTGCACCAA GTGGACACTT TCATCTTTTG CCGAGAAACA CTATGCTCCT
    TTCCTCTTGA AACCAAAAGC CAAGGTCGTG GTCATCTTCC TTTTCCTGGG CTTGCTGGGG
    GTCAGCCTTT ATGGGACCAC CCGAGTGCGC GATGGGCTGG ACCTCACAGA CATTGTGCCC
    CGGGAAACCA GAGAATATGA TTTTATTGCT GCACAGTTCA AATACTTCTC TTTCTACAAC
    ATGTATATAG TCACCCAGAA AGCAGACTAC CCAAATATCC AGCACTTACT TTACGACCTT
    CACAAGAGTT TCAGCAATGT GAAGTATGTC ATGTTGGAAG AAAATAAACA GCTTCCCAAA
    ATGTGGCTGC ATTACTTCCG AGACTGGTTA CAAGGACTTC AGGACGCATT TGACAGTGAC
    TGGGAAACTG GAAAANNTCA TGCCAACAAA TTCAAAAAAG GATCAGATGA TGGGGTCCTT
    GCCTACAAAC TCCTGGTGCA GACCGGCAGC CGCGATAAAC CCATCGACAT CAGTCAGTTG
    ACCAAGCAGC GCCTGGTGGA TGCACATGGC ATCATCAACC CCAGTGCATT CTACATCTAC
    CTGACTGCCT GGGTGAGCAA TGACCCTGTG GCTTATGCCG CCTCCCAGGC CAACATCCGG
    CCTCACCGTC CTGAGTGGGT GCATGACAAA GCTGACTACA TGCCCGAAAC CAGGCTCAGA
    ATCCCAGCAG CAGAGCCCAT CGAATACGCT CAGTTCCCTT TCTACCTCAA TGGCTTGCGG
    GACACATCTG ACTTCGTGGA AGCTATTGAA AAGGTCCGAA GCATCTGCAA CAACTACACC
    AGCCTGGGGC TGTCCAGCTA CCCCAACGGC TACCCCTTCC TCTTCTGGGA GCAGTACATT
    GGCCTCCGCC ACTGGCTTCT GTTGTCTGTC AGCGTGGTGC TGGCCTGCAC GTTCCTCGTA
    TGCGCCGTCT TCCTCCTGAA CCCCTGGACG GCCGGGATCA TTGTGACGGT GCTGGCTTTG
    ATGACAGTCG AGCTCTTTGG CATGATGGGC CTCATTGGAA TCAAGCTGAG TGCTGTGCCA
    GTGGTCATCC TGATCGCTTC TGTCGGCATA GGAGTGGAGT TCACCGTTCA CGTTGCTCTG
    GCTTTCCTGA CAGCCATTGG TGATAAGAAC CGCAGAGCCG TGCTGGCGCT GGAGCACATG
    TTCGCGCCCG TCCTGGATGG CGCCGTGTCC ACTCTGCTGG GAGTGCTGAT GCTGGCGGGC
    TCGGAGTTTG ATTTCATTGT CAGGTATTTC TTTGCTGTTC TGGCGATCCT AACAATCCTG
    GGTGTTCTCA ACGGGCTGGT TTTGCTGCCA GTCCTTCTGT CCTTCTTCGG ACCGTATCCT
    GAGGTATCTC CAGCCAATGG GCTGAACCGA CTGCCCACCC CTTCCCCTGA GCCACCCCCC
    AGTGTGGTCC GGTTTGCCGT GCCACCCAGC CATGCAAACA ATGGGTCTGA TTCCTCTGAC
    TCGGAGTACA GTTCTCAGAC TACAGTGTCG GGCATCAGTG AAGAGCTTCG GCACTACGAG
    GCCCAGCAGG GCACCAGGGG CCCTGCCCAC CAGGTGATCG TGGAAGCCAC AGAAAACCCC
    GTCTTTGCCC GCTCCACTGT GGTCCATCCC GAAGCGAGAC ATCACCCACC CTCAAACCCT
    CGACAGCAGC CCCCCCTGGA CTCAGAGCCC CTTCCTCCTG GGCGACCAGG CCAGCAGCCC
    CGGAGGGAAC CCCCCAGAGA AGGCTTGCGG CCGCCCCCCT ACAGACCGCG CAGAGACGCT
    TTTGAAATTT CTACTGACGG GCATCCTGGC CCTGGCAGTA GGGACCGTGC GGGCCCCCGG
    GGAGCCCGTT CTAACAACCC CCGGCACCCA GTGTTCACTG CCATGGGCAG CTCCGCGCCC
    AGCTACTGCC AGCCCATCAC CACTGTGACG GCCTCGGCGT CCGTGACTGT TGCTGTGCAC
    CCCCCGCCTG CGCCTGGGCC TGGCTCTGCT TGGAACCCCA GAGGAGGACT CTGTCCGGGC
    TACGGGGACT GCCCGGAGAC TGACCACAGG CTATTTGAGG ACCCCCACGT GCCTTTTAAC
    GTCCGGTGCG AGAGGAGGGA TTCCAAGGTG GAAGTCATAG AGCTGCAGGA TGTGGAATGT
    GAGGAGAGAC CCCGGGGCGG TAGCTCCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003638919.1
    CDS48..4400
    Translation

    Target ORF information:

    RefSeq Version XM_003638871.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris patched 1 (PTCH1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003638871.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCGGAGCCC CAGGACCGCG GCGGTGGCGG CGGCAGCGGG 
    GCCCCCGGCC GGCCGGCCGG CGGCGGGAGA CGCAGACGGG CCGGGGGGCT GCGCCGCAGC
    GCCGCGCTGG ACCGGGACTA TCTGCACCGG CCCAGCTACT GCGACGCCGC CTTCGCTCTG
    GAGCAGATTT CCAAGGGGAA AGCTACTGGC CGGAAAGCGC CGCTGTGGCT GAGAGCGAAG
    TTTCAGAGAC TCTTATTTAA ACTGGGTTGT TACATTCAAA AAAACTGCGG CAAGTTCTTG
    GTTGTGGGCC TCCTCATATT TGGGGCCTTC GCTGTGGGAT TAAAGGCAGC TAATCTCGAG
    ACCAACGTGG AGGAGCTGTG GGTGGAAGTT GGTGGACGAG TAAGTCGTGA ATTAAATTAT
    ACTCGCCAGA AGATTGGAGA AGAGGCTATG TTTAATCCTC AACTCATGAT TCAGACCCCG
    AAAGAAGAAG GTGCTAACGT CCTGACCACA GAAGCTCTCC GACAACACCT GGACTCAGCG
    CTCCAGGCCA GCCGGGTCCA TGTGTATATG TATAACAGGC AGTGGAAATT GGAACATTTG
    TGTTACAAAT CTGGAGAACT TATCACAGAA ACAGGTTACA TGGATCAGAT AATCGAATAT
    CTTTACCCTT GTTTAATTAT TACACCTTTG GACTGCTTCT GGGAAGGGGC AAAGTTACAA
    TCTGGGACAG CATACCTACT AGGTAAGCCT CCTTTGCAGT GGACAAACTT TGACCCTTTG
    GAATTCCTGG AAGAGTTAAA GAAAATAAAC TATCAAGTGG ACAGCTGGGA GGAAATGCTG
    AATAAAGCTG AAGTTGGTCA TGGTTACATG GACCGGCCCT GCCTCAACCC GGCTGATCCG
    GACTGCCCCG CCACAGCCCC CAACAAAAAT GCAACCAAAC CTCTTGATGT GGCCCTTGTT
    TTGAATGGTG GATGTCATGG ATTATCCAGA AAGTACATGC ACTGGCAGGA GGAACTGATT
    GTGGGTGGCA CAGTCAAGAA CAGCACCGGG AAGCTTGTCA GCGCCCACGC CCTGCAAACC
    ATGTTTCAGT TAATGACTCC CAAGCAAATG TACGAACACT TCAAGGGGTA TGAGTATGTC
    TCACACATTA ACTGGAATGA GGACAAGGCA GCAGCCATCC TGGAGGCTTG GCAGAGGACA
    TATGTGGAGG TGGTTCATCA AAGTGTCGCT CCAAACTCCA CTCAGAAAGT GCTTTCCTTC
    ACCACCACAA CCCTGGATGA CATCCTGAAG TCCTTCTCTG ATGTCAGCGT CATCCGAGTG
    GCTAGTGGCT ACCTCCTGAT GCTTGCCTAT GCCTGTTTAA CCATGCTGCG CTGGGATTGC
    TCCAAGTCCC AGGGTGCCGT GGGGCTGGCT GGCGTCCTGT TGGTTGCACT GTCAGTGGCT
    GCAGGATTGG GCCTGTGCTC ATTGATCGGG ATTTCCTTTA ACGCTGCAAC AACTCAGGTT
    TTGCCATTTC TCGCTCTTGG TGTTGGTGTG GATGATGTTT TCCTTCTGGC ACATGCATTT
    AGTGAAACAG GACAGAATAA AAGAATCCCT TTTGAGGATA GGACTGGGGA GTGCCTGAAG
    CGCACAGGAG CCAGTGTGGC GCTCACCTCC ATCAGCAATG TCACAGCCTT CTTCATGGCT
    GCTCTGATCC CTATTCCCGC TCTGCGAGCC TTCTCCCTTC AGGCGGCTGT AGTAGTGGTA
    TTCAATTTTG CTATGGTTCT GCTCATTTTT CCTGCAATTC TCAGCATGGA TTTGTATCGA
    CGTGAGGACA GGAGATTGGA TATTTTCTGC TGTTTTACAA GCCCCTGCGT CAGCAGAGTG
    ATTCAGGTTG AACCTCAGGC CTACACGGAG ACTCACGATA ACACTCGCTA CAGTCCCCCG
    CCCCCCTACA GCAGCCACAG CTTCACCCAC GAAACCCAGA TCACCATGCA GTCCACAGTA
    CAGCTCCGCA CAGAGTATGA CCCCCACACG CACGTGTACT ACACCACCGC CGAGCCACGC
    TCTGAGATCT CCGTGCAGCC CGTCACCATG ACCCAGGATA CCCTCAGCTG CCAGAGCCCT
    GAGAGCACCA GCTCCACGAG GGACCTGCTC TCCCAGTTCT CTGACTCCAG CCTCCACTGC
    CTTGAGCCCC CTTGCACCAA GTGGACACTT TCATCTTTTG CCGAGAAACA CTATGCTCCT
    TTCCTCTTGA AACCAAAAGC CAAGGTCGTG GTCATCTTCC TTTTCCTGGG CTTGCTGGGG
    GTCAGCCTTT ATGGGACCAC CCGAGTGCGC GATGGGCTGG ACCTCACAGA CATTGTGCCC
    CGGGAAACCA GAGAATATGA TTTTATTGCT GCACAGTTCA AATACTTCTC TTTCTACAAC
    ATGTATATAG TCACCCAGAA AGCAGACTAC CCAAATATCC AGCACTTACT TTACGACCTT
    CACAAGAGTT TCAGCAATGT GAAGTATGTC ATGTTGGAAG AAAATAAACA GCTTCCCAAA
    ATGTGGCTGC ATTACTTCCG AGACTGGTTA CAAGGACTTC AGGACGCATT TGACAGTGAC
    TGGGAAACTG GAAAANNTCA TGCCAACAAA TTCAAAAAAG GATCAGATGA TGGGGTCCTT
    GCCTACAAAC TCCTGGTGCA GACCGGCAGC CGCGATAAAC CCATCGACAT CAGTCAGTTG
    ACCAAGCAGC GCCTGGTGGA TGCACATGGC ATCATCAACC CCAGTGCATT CTACATCTAC
    CTGACTGCCT GGGTGAGCAA TGACCCTGTG GCTTATGCCG CCTCCCAGGC CAACATCCGG
    CCTCACCGTC CTGAGTGGGT GCATGACAAA GCTGACTACA TGCCCGAAAC CAGGCTCAGA
    ATCCCAGCAG CAGAGCCCAT CGAATACGCT CAGTTCCCTT TCTACCTCAA TGGCTTGCGG
    GACACATCTG ACTTCGTGGA AGCTATTGAA AAGGTCCGAA GCATCTGCAA CAACTACACC
    AGCCTGGGGC TGTCCAGCTA CCCCAACGGC TACCCCTTCC TCTTCTGGGA GCAGTACATT
    GGCCTCCGCC ACTGGCTTCT GTTGTCTGTC AGCGTGGTGC TGGCCTGCAC GTTCCTCGTA
    TGCGCCGTCT TCCTCCTGAA CCCCTGGACG GCCGGGATCA TTGTGACGGT GCTGGCTTTG
    ATGACAGTCG AGCTCTTTGG CATGATGGGC CTCATTGGAA TCAAGCTGAG TGCTGTGCCA
    GTGGTCATCC TGATCGCTTC TGTCGGCATA GGAGTGGAGT TCACCGTTCA CGTTGCTCTG
    GCTTTCCTGA CAGCCATTGG TGATAAGAAC CGCAGAGCCG TGCTGGCGCT GGAGCACATG
    TTCGCGCCCG TCCTGGATGG CGCCGTGTCC ACTCTGCTGG GAGTGCTGAT GCTGGCGGGC
    TCGGAGTTTG ATTTCATTGT CAGGTATTTC TTTGCTGTTC TGGCGATCCT AACAATCCTG
    GGTGTTCTCA ACGGGCTGGT TTTGCTGCCA GTCCTTCTGT CCTTCTTCGG ACCGTATCCT
    GAGGTATCTC CAGCCAATGG GCTGAACCGA CTGCCCACCC CTTCCCCTGA GCCACCCCCC
    AGTGTGGTCC GGTTTGCCGT GCCACCCAGC CATGCAAACA ATGGGTCTGA TTCCTCTGAC
    TCGGAGTACA GTTCTCAGAC TACAGTGTCG GGCATCAGTG AAGAGCTTCG GCACTACGAG
    GCCCAGCAGG GCACCAGGGG CCCTGCCCAC CAGGTGATCG TGGAAGCCAC AGAAAACCCC
    GTCTTTGCCC GCTCCACTGT GGTCCATCCC GAAGCGAGAC ATCACCCACC CTCAAACCCT
    CGACAGCAGC CCCCCCTGGA CTCAGAGCCC CTTCCTCCTG GGCGACCAGG CCAGCAGCCC
    CGGAGGGAAC CCCCCAGAGA AGGCTTGCGG CCGCCCCCCT ACAGACCGCG CAGAGACGCT
    TTTGAAATTT CTACTGACGG GCATCCTGGC CCTGGCAGTA GGGACCGTGC GGGCCCCCGG
    GGAGCCCGTT CTAACAACCC CCGGCACCCA GTGTTCACTG CCATGGGCAG CTCCGCGCCC
    AGCTACTGCC AGCCCATCAC CACTGTGACG GCCTCGGCGT CCGTGACTGT TGCTGTGCAC
    CCCCCGCCTG CGCCTGGGCC TGGCTCTGCT TGGAACCCCA GAGGAGGACT CTGTCCGGGC
    TACGGGGACT GCCCGGAGAC TGACCACAGG CTATTTGAGG ACCCCCACGT GCCTTTTAAC
    GTCCGGTGCG AGAGGAGGGA TTCCAAGGTG GAAGTCATAG AGCTGCAGGA TGTGGAATGT
    GAGGAGAGAC CCCGGGGCGG TAGCTCCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.