MBD5 cDNA ORF clone, Canis lupus familiaris(dog)

The following MBD5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe295176 XM_022406168.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe21068 XM_022406167.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe295174 XM_022406165.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe295175 XM_022406166.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41423 XM_022406157.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe41423 XM_022406156.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe41423 XM_022406159.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe41423 XM_022406161.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe41423 XM_014121498.2
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe41423 XM_022406158.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe41423 XM_022406160.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe41423 XM_022406152.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe41423 XM_022406153.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe41423 XM_022406162.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe41423 XM_022406155.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe41423 XM_022406163.1
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe21067 XM_005631969.3
Latest version!
Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OCe21067 XM_005631969.2 Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe41423 XM_014121498.1 Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe295176
    Clone ID Related Accession (Same CDS sequence) XM_022406168.1
    Accession Version XM_022406168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGGAGCCC
    GAGAAGTTGA AGACACTAAC AGAAGGTTTA GAAGCCTACA GCCGAGCCCG GAAAAGGAAC
    AGAAAAAGTG GAAAGCTAAA TAACCATTTA GAAGCTGCTA TTCATGAGGC CATGAGTGAA
    CTGGACAAAA TGTCTGGGAC TGTTCACCAA ATCCCACAGG GAGACAGACA AATGAGACCC
    CCCAAACCCA AGAGGAGGAA GATCTCCAGA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261876.1
    CDS1564..5556
    Translation

    Target ORF information:

    RefSeq Version XM_022406168.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGGAGCCC
    GAGAAGTTGA AGACACTAAC AGAAGGTTTA GAAGCCTACA GCCGAGCCCG GAAAAGGAAC
    AGAAAAAGTG GAAAGCTAAA TAACCATTTA GAAGCTGCTA TTCATGAGGC CATGAGTGAA
    CTGGACAAAA TGTCTGGGAC TGTTCACCAA ATCCCACAGG GAGACAGACA AATGAGACCC
    CCCAAACCCA AGAGGAGGAA GATCTCCAGA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe21068
    Clone ID Related Accession (Same CDS sequence) XM_022406167.1
    Accession Version XM_022406167.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGACATGTC CTCAATAAAC
    AATACTTTGA ATAACCATCA ACTGACTCAT CTGCAGTCGC TGTTAAACAA CAATCAGATG
    TTTCCTCCAA ATCAGCAGCA GCAGCAGCAG CTTCTTCAAG GGTACCAGAA CCTGCAGGCA
    TTTCAAGGAC AGCCCACAAT TCCTTGCCCG GCTAACAGTA ACCCCATGGC TTGTCTGTTT
    CAGAACTTTC AGGTGAGAAT GCAGGAAGAT GCAGCTCTCC TAAACAAAAG AATAAGCTCT
    CAGCCGGGGC TCACGGCACT TCCTGAGAAT CCAAACACTA CACTTCCACC TTTCCAAGAT
    ACATCTTGTG AGTTGCAACC GAGGATTGAC CCATCTCTTG GTCAGCAGGT GAAAGATGGC
    CTCATTGTGG GTGGCCAAGG TGATGCTTCT GTAGATGCCA TTTACAAAGC AGTTGTTGAT
    GCAGCCAGCA AAGGAATGCA GGTTGTCATC ACCACTGCAG TCAACAGTAC AACTCAGATC
    AGCCCCATTC CAGCTCTGAG TGCCATGAGT GCCTTCACTG CCTCGATTGG TGACCCGTTA
    AGTCTCCCCA GTGCTGTCAG TGCGGTCATT CATGGACGAA ACATGGGCAG TGTCGATCAT
    GATGGTAGGC TGAGGAATGC AAGAGGGGCT CGGCTGCCCA GGAATCTGGA CCATGGGAAA
    AACTCAAATG AAGGAGATGG GTTTGAATAT TTCAAGTCAG CAAGTTGCCA CACATCCAAA
    AAACAGTGGG ATGGGGAGCA AAGCCCTGGA GGGGAGCGAA ACAGGTGGAA GTGTGAGGAA
    TTTTTAGATC ATCCAGGCCA TATCCACAGT AGTCCTTGTC ACGAAAGGTC CAACAATGTC
    TCTACACTGC CATTTCTGCC TGGGGAACAG CACCCGGTAC TGTTACCACC AAGAAACTGT
    CAAGGGGATA AAATTCTGGA GGAAAATTTC AGGTATAATA ACTACAAAAG AACTATGATG
    AGTTTTAAGG AGAGACTAGA GAACACTGTG GAAAGATGTG CACACATTAA TGGGAACAGA
    CCTCGACAGA GTCGGGGCTT TGGAGAGCTG CTGAGTACCA CAAGACAAGA CCTGGTCCTA
    GAGGAGCAGT CTCCGAGCTC CTCAAATAGT TTGGAAAATT CTCTAGTCAA AGACTACATC
    CATTACAATG GAGACTTTAA TGCCAAAAGC ATTAATGGGT GTGTGCCTAG CCCTTCAGAT
    GCTAAAAGCA TTAGTAGTGA AGATGACCTA AGGAATCCAG ATTCCCCTTC TTCAAATGAG
    TTGATACATT ATAGGCCAAG GACGTTCAAC GTTGGCGACT TGGTCTGGGG CCAAATCAAA
    GGACTTACTT CTTGGCCTGG AAAATTAGTA AGAGAAGACG ACGTTCACAA TTCATGTCAA
    CAAAGCCCCG AGGAAGGGAA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTA
    GAAGCCTACA GCCGAGCCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA
    GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTTCACCAA
    ATCCCACAGG GAGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261875.1
    CDS1564..6066
    Translation

    Target ORF information:

    RefSeq Version XM_022406167.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGACATGTC CTCAATAAAC
    AATACTTTGA ATAACCATCA ACTGACTCAT CTGCAGTCGC TGTTAAACAA CAATCAGATG
    TTTCCTCCAA ATCAGCAGCA GCAGCAGCAG CTTCTTCAAG GGTACCAGAA CCTGCAGGCA
    TTTCAAGGAC AGCCCACAAT TCCTTGCCCG GCTAACAGTA ACCCCATGGC TTGTCTGTTT
    CAGAACTTTC AGGTGAGAAT GCAGGAAGAT GCAGCTCTCC TAAACAAAAG AATAAGCTCT
    CAGCCGGGGC TCACGGCACT TCCTGAGAAT CCAAACACTA CACTTCCACC TTTCCAAGAT
    ACATCTTGTG AGTTGCAACC GAGGATTGAC CCATCTCTTG GTCAGCAGGT GAAAGATGGC
    CTCATTGTGG GTGGCCAAGG TGATGCTTCT GTAGATGCCA TTTACAAAGC AGTTGTTGAT
    GCAGCCAGCA AAGGAATGCA GGTTGTCATC ACCACTGCAG TCAACAGTAC AACTCAGATC
    AGCCCCATTC CAGCTCTGAG TGCCATGAGT GCCTTCACTG CCTCGATTGG TGACCCGTTA
    AGTCTCCCCA GTGCTGTCAG TGCGGTCATT CATGGACGAA ACATGGGCAG TGTCGATCAT
    GATGGTAGGC TGAGGAATGC AAGAGGGGCT CGGCTGCCCA GGAATCTGGA CCATGGGAAA
    AACTCAAATG AAGGAGATGG GTTTGAATAT TTCAAGTCAG CAAGTTGCCA CACATCCAAA
    AAACAGTGGG ATGGGGAGCA AAGCCCTGGA GGGGAGCGAA ACAGGTGGAA GTGTGAGGAA
    TTTTTAGATC ATCCAGGCCA TATCCACAGT AGTCCTTGTC ACGAAAGGTC CAACAATGTC
    TCTACACTGC CATTTCTGCC TGGGGAACAG CACCCGGTAC TGTTACCACC AAGAAACTGT
    CAAGGGGATA AAATTCTGGA GGAAAATTTC AGGTATAATA ACTACAAAAG AACTATGATG
    AGTTTTAAGG AGAGACTAGA GAACACTGTG GAAAGATGTG CACACATTAA TGGGAACAGA
    CCTCGACAGA GTCGGGGCTT TGGAGAGCTG CTGAGTACCA CAAGACAAGA CCTGGTCCTA
    GAGGAGCAGT CTCCGAGCTC CTCAAATAGT TTGGAAAATT CTCTAGTCAA AGACTACATC
    CATTACAATG GAGACTTTAA TGCCAAAAGC ATTAATGGGT GTGTGCCTAG CCCTTCAGAT
    GCTAAAAGCA TTAGTAGTGA AGATGACCTA AGGAATCCAG ATTCCCCTTC TTCAAATGAG
    TTGATACATT ATAGGCCAAG GACGTTCAAC GTTGGCGACT TGGTCTGGGG CCAAATCAAA
    GGACTTACTT CTTGGCCTGG AAAATTAGTA AGAGAAGACG ACGTTCACAA TTCATGTCAA
    CAAAGCCCCG AGGAAGGGAA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTA
    GAAGCCTACA GCCGAGCCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA
    GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTTCACCAA
    ATCCCACAGG GAGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe295174
    Clone ID Related Accession (Same CDS sequence) XM_022406165.1
    Accession Version XM_022406165.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5115bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    ATTCTCTCAG TGATGCATAA AGAAGCGCAC AACCATCCCC ATGGTACAGA TGAGGGAGCT
    GAAACTCAGC GAAGTAGCAT TCCTAAGGAC ACAAGTGACA GGCAGTTAAG CCAGGACCCA
    ACCCCCGTCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261873.1
    CDS1564..6678
    Translation

    Target ORF information:

    RefSeq Version XM_022406165.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406165.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    ATTCTCTCAG TGATGCATAA AGAAGCGCAC AACCATCCCC ATGGTACAGA TGAGGGAGCT
    GAAACTCAGC GAAGTAGCAT TCCTAAGGAC ACAAGTGACA GGCAGTTAAG CCAGGACCCA
    ACCCCCGTCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe295175
    Clone ID Related Accession (Same CDS sequence) XM_022406166.1
    Accession Version XM_022406166.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGACATGTC CTCAATAAAC
    AATACTTTGA ATAACCATCA ACTGACTCAT CTGCAGTCGC TGTTAAACAA CAATCAGATG
    TTTCCTCCAA ATCAGCAGCA GCAGCAGCAG CTTCTTCAAG GGTACCAGAA CCTGCAGGCA
    TTTCAAGGAC AGCCCACAAT TCCTTGCCCG GCTAACAGTA ACCCCATGGC TTGTCTGTTT
    CAGAACTTTC AGGTGAGAAT GCAGGAAGAT GCAGCTCTCC TAAACAAAAG AATAAGCTCT
    CAGCCGGGGC TCACGGCACT TCCTGAGAAT CCAAACACTA CACTTCCACC TTTCCAAGAT
    ACATCTTGTG AGTTGCAACC GAGGATTGAC CCATCTCTTG GTCAGCAGGT GAAAGATGGC
    CTCATTGTGG GTGGCCAAGG TGATGCTTCT GTAGATGCCA TTTACAAAGC AGTTGTTGAT
    GCAGCCAGCA AAGGAATGCA GGTTGTCATC ACCACTGCAG TCAACAGTAC AACTCAGATC
    AGCCCCATTC CAGCTCTGAG TGCCATGAGT GCCTTCACTG CCTCGATTGG TGACCCGTTA
    AGTCTCCCCA GTGCTGTCAG TGCGGTCATT CATGGACGAA ACATGGGCAG TGTCGATCAT
    GATGGTAGGC TGAGGAATGC AAGAGGGGCT CGGCTGCCCA GGAATCTGGA CCATGGGAAA
    AACTCAAATG AAGGAGATGG GTTTGAATAT TTCAAGTCAG CAAGTTGCCA CACATCCAAA
    AAACAGTGGG ATGGGGAGCA AAGCCCTGGA GGGGAGCGAA ACAGGTGGAA GTGTGAGGAA
    TTTTTAGATC ATCCAGGCCA TATCCACAGT AGTCCTTGTC ACGAAAGGTC CAACAATGTC
    TCTACACTGC CATTTCTGCC TGGGGAACAG CACCCGGTAC TGTTACCACC AAGAAACTGT
    CAAGGGGATA AAATTCTGGA GGAAAATTTC AGGTATAATA ACTACAAAAG AACTATGATG
    AGTTTTAAGG AGAGACTAGA GAACACTGTG GAAAGATGTG CACACATTAA TGGGAACAGA
    CCTCGACAGA GTCGGGGCTT TGGAGAGCTG CTGAGTACCA CAAGACAAGA CCTGGTCCTA
    GAGGAGCAGT CTCCGAGCTC CTCAAATAGT TTGGAAAATT CTCTAGTCAA AGACTACATC
    CATTACAATG GAGACTTTAA TGCCAAAAGC ATTAATGGGT GTGTGCCTAG CCCTTCAGAT
    GCTAAAAGCA TTAGTAGTGA AGATGACCTA AGGAATCCAG ATTCCCCTTC TTCAAATGAG
    TTGATACATT ATAGGCCAAG GACGTTCAAC GTTGGCGACT TGGTCTGGGG CCAAATCAAA
    GGACTTACTT CTTGGCCTGG AAAATTAGTA AGAGAAGACG ACGTTCACAA TTCATGTCAA
    CAAAGCCCCG AGGAAGGGAA GGCCGTACAG GTGGTCCCTG GCCTGACAGA TCAATGCTGT
    CCCTGGACTA CTGCCGCGGG CCGAGGTGTC CATGCTGTCC CTAAGTTGAT GACGCTTCAG
    CCAGCTGTCA TGATAGTCAA CGACCTGGTG GAGCCCGAGA AGTTGAAGAC ACTAACAGAA
    GGTTTAGAAG CCTACAGCCG AGCCCGGAAA AGGAACAGAA AAAGTGGAAA GCTAAATAAC
    CATTTAGAAG CTGCTATTCA TGAGGCCATG AGTGAACTGG ACAAAATGTC TGGGACTGTT
    CACCAAATCC CACAGGGAGA CAGACAAATG AGACCCCCCA AACCCAAGAG GAGGAAGATC
    TCCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261874.1
    CDS1564..6192
    Translation

    Target ORF information:

    RefSeq Version XM_022406166.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406166.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GTGACATGTC CTCAATAAAC
    AATACTTTGA ATAACCATCA ACTGACTCAT CTGCAGTCGC TGTTAAACAA CAATCAGATG
    TTTCCTCCAA ATCAGCAGCA GCAGCAGCAG CTTCTTCAAG GGTACCAGAA CCTGCAGGCA
    TTTCAAGGAC AGCCCACAAT TCCTTGCCCG GCTAACAGTA ACCCCATGGC TTGTCTGTTT
    CAGAACTTTC AGGTGAGAAT GCAGGAAGAT GCAGCTCTCC TAAACAAAAG AATAAGCTCT
    CAGCCGGGGC TCACGGCACT TCCTGAGAAT CCAAACACTA CACTTCCACC TTTCCAAGAT
    ACATCTTGTG AGTTGCAACC GAGGATTGAC CCATCTCTTG GTCAGCAGGT GAAAGATGGC
    CTCATTGTGG GTGGCCAAGG TGATGCTTCT GTAGATGCCA TTTACAAAGC AGTTGTTGAT
    GCAGCCAGCA AAGGAATGCA GGTTGTCATC ACCACTGCAG TCAACAGTAC AACTCAGATC
    AGCCCCATTC CAGCTCTGAG TGCCATGAGT GCCTTCACTG CCTCGATTGG TGACCCGTTA
    AGTCTCCCCA GTGCTGTCAG TGCGGTCATT CATGGACGAA ACATGGGCAG TGTCGATCAT
    GATGGTAGGC TGAGGAATGC AAGAGGGGCT CGGCTGCCCA GGAATCTGGA CCATGGGAAA
    AACTCAAATG AAGGAGATGG GTTTGAATAT TTCAAGTCAG CAAGTTGCCA CACATCCAAA
    AAACAGTGGG ATGGGGAGCA AAGCCCTGGA GGGGAGCGAA ACAGGTGGAA GTGTGAGGAA
    TTTTTAGATC ATCCAGGCCA TATCCACAGT AGTCCTTGTC ACGAAAGGTC CAACAATGTC
    TCTACACTGC CATTTCTGCC TGGGGAACAG CACCCGGTAC TGTTACCACC AAGAAACTGT
    CAAGGGGATA AAATTCTGGA GGAAAATTTC AGGTATAATA ACTACAAAAG AACTATGATG
    AGTTTTAAGG AGAGACTAGA GAACACTGTG GAAAGATGTG CACACATTAA TGGGAACAGA
    CCTCGACAGA GTCGGGGCTT TGGAGAGCTG CTGAGTACCA CAAGACAAGA CCTGGTCCTA
    GAGGAGCAGT CTCCGAGCTC CTCAAATAGT TTGGAAAATT CTCTAGTCAA AGACTACATC
    CATTACAATG GAGACTTTAA TGCCAAAAGC ATTAATGGGT GTGTGCCTAG CCCTTCAGAT
    GCTAAAAGCA TTAGTAGTGA AGATGACCTA AGGAATCCAG ATTCCCCTTC TTCAAATGAG
    TTGATACATT ATAGGCCAAG GACGTTCAAC GTTGGCGACT TGGTCTGGGG CCAAATCAAA
    GGACTTACTT CTTGGCCTGG AAAATTAGTA AGAGAAGACG ACGTTCACAA TTCATGTCAA
    CAAAGCCCCG AGGAAGGGAA GGCCGTACAG GTGGTCCCTG GCCTGACAGA TCAATGCTGT
    CCCTGGACTA CTGCCGCGGG CCGAGGTGTC CATGCTGTCC CTAAGTTGAT GACGCTTCAG
    CCAGCTGTCA TGATAGTCAA CGACCTGGTG GAGCCCGAGA AGTTGAAGAC ACTAACAGAA
    GGTTTAGAAG CCTACAGCCG AGCCCGGAAA AGGAACAGAA AAAGTGGAAA GCTAAATAAC
    CATTTAGAAG CTGCTATTCA TGAGGCCATG AGTGAACTGG ACAAAATGTC TGGGACTGTT
    CACCAAATCC CACAGGGAGA CAGACAAATG AGACCCCCCA AACCCAAGAG GAGGAAGATC
    TCCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_022406152.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261860.1
    CDS1564..6891
    Translation

    Target ORF information:

    RefSeq Version XM_022406152.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406152.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_022406157.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261865.1
    CDS1322..6649
    Translation

    Target ORF information:

    RefSeq Version XM_022406157.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406157.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_022406156.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261864.1
    CDS1465..6792
    Translation

    Target ORF information:

    RefSeq Version XM_022406156.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_022406158.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261866.1
    CDS1223..6550
    Translation

    Target ORF information:

    RefSeq Version XM_022406158.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406158.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_022406162.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261870.1
    CDS1094..6421
    Translation

    Target ORF information:

    RefSeq Version XM_022406162.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406162.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_014121498.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976973.1
    CDS778..6105
    Translation

    Target ORF information:

    RefSeq Version XM_014121498.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121498.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_022406153.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261861.1
    CDS1439..6766
    Translation

    Target ORF information:

    RefSeq Version XM_022406153.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_022406155.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261863.1
    CDS1510..6837
    Translation

    Target ORF information:

    RefSeq Version XM_022406155.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_022406160.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261868.1
    CDS1336..6663
    Translation

    Target ORF information:

    RefSeq Version XM_022406160.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406160.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_014121498.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976973.1
    CDS758..6085
    Translation

    Target ORF information:

    RefSeq Version XM_014121498.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121498.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_022406159.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261867.1
    CDS1268..6595
    Translation

    Target ORF information:

    RefSeq Version XM_022406159.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406159.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_022406161.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261869.1
    CDS1101..6428
    Translation

    Target ORF information:

    RefSeq Version XM_022406161.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406161.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41423
    Clone ID Related Accession (Same CDS sequence) XM_022406152.1 , XM_022406157.1 , XM_022406156.1 , XM_022406158.1 , XM_022406162.1 , XM_014121498.2 , XM_022406153.1 , XM_022406155.1 , XM_022406160.1 , XM_014121498.1 , XM_022406159.1 , XM_022406161.1 , XM_022406163.1
    Accession Version XM_022406163.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261871.1
    CDS1049..6376
    Translation

    Target ORF information:

    RefSeq Version XM_022406163.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022406163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GCCGTACAGG TGGTCCCTGG CCTGACAGAT CAATGCTGTC CCTGGACTAC TGCCGCGGGC
    CGAGGTGTCC ATGCTGTCCC TAAGTTGATG ACGCTTCAGC CAGCTGTCAT GATAGTCAAC
    GACCTGGTGG AGCCCGAGAA GTTGAAGACA CTAACAGAAG GTTTAGAAGC CTACAGCCGA
    GCCCGGAAAA GGAACAGAAA AAGTGGAAAG CTAAATAACC ATTTAGAAGC TGCTATTCAT
    GAGGCCATGA GTGAACTGGA CAAAATGTCT GGGACTGTTC ACCAAATCCC ACAGGGAGAC
    AGACAAATGA GACCCCCCAA ACCCAAGAGG AGGAAGATCT CCAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe21067
    Clone ID Related Accession (Same CDS sequence) XM_005631969.2 , XM_005631969.3
    Accession Version XM_005631969.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product methyl-CpG-binding domain protein 5 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726094.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GTGGAGCCCG AGAAGTTGAA GACACTAACA GAAGGTTTAG AAGCCTACAG CCGAGCCCGG
    AAAAGGAACA GAAAAAGTGG AAAGCTAAAT AACCATTTAG AAGCTGCTAT TCATGAGGCC
    ATGAGTGAAC TGGACAAAAT GTCTGGGACT GTTCACCAAA TCCCACAGGG AGACAGACAA
    ATGAGACCCC CCAAACCCAA GAGGAGGAAG ATCTCCAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005632026.1
    CDS1513..6714
    Translation

    Target ORF information:

    RefSeq Version XM_005631969.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631969.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GTGGAGCCCG AGAAGTTGAA GACACTAACA GAAGGTTTAG AAGCCTACAG CCGAGCCCGG
    AAAAGGAACA GAAAAAGTGG AAAGCTAAAT AACCATTTAG AAGCTGCTAT TCATGAGGCC
    ATGAGTGAAC TGGACAAAAT GTCTGGGACT GTTCACCAAA TCCCACAGGG AGACAGACAA
    ATGAGACCCC CCAAACCCAA GAGGAGGAAG ATCTCCAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe21067
    Clone ID Related Accession (Same CDS sequence) XM_005631969.2 , XM_005631969.3
    Accession Version XM_005631969.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product methyl-CpG-binding domain protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006601.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631969.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GTGGAGCCCG AGAAGTTGAA GACACTAACA GAAGGTTTAG AAGCCTACAG CCGAGCCCGG
    AAAAGGAACA GAAAAAGTGG AAAGCTAAAT AACCATTTAG AAGCTGCTAT TCATGAGGCC
    ATGAGTGAAC TGGACAAAAT GTCTGGGACT GTTCACCAAA TCCCACAGGG AGACAGACAA
    ATGAGACCCC CCAAACCCAA GAGGAGGAAG ATCTCCAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005632026.1
    CDS1564..6765
    Translation

    Target ORF information:

    RefSeq Version XM_005631969.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris methyl-CpG binding domain protein 5 (MBD5), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631969.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAGGACTG TGACGGAGGG GACAAAGATG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG GCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA CGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTGT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACTGC AGAAGATGTT AAAGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATCAT TGCCGTGGCC ACACTTCACA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGGGGA GGAACAAATG CAACTCCAGT AGTACCTTCA
    CGGGCAGCAA CTCCAAGATC CGTAAGAAAT AAGTCGCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATCGGAT CATCCAATGC CATGGGAAGG
    TTATATGTAC AAGAACTGCC TGGAAGCCAG CAACAAGAAC TCCACCCTGT CTACCCCCGG
    CAGAGATTGG GCAGCAGTGA ACACGGACAG AAATCTCCAT TCCGTGGCAG CCACGGAGGC
    CTGCCCAGCC CAGCATCGTC AGGTTCCCAG ATATATGGAG ACGGCTCAAT CTCTCCAAGG
    ACTGACCCAC TCGGAAGCCC AGATGTCTTC ACAAGAAATA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA TTTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCGGTGAAT CAAAACCCTG TTATCATTAA TCCAACTAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT GTGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCTTTGCCA ACCGTAAAAC CTGGTCATAT GAATCATGGG
    AGTCATATAC AAAGAGTTCA GCATTCAGCT TCAACCTCCT TGTCCCCTTC TCCAGTGACA
    TCCCCAGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCACGGA AATTTCATGA TGCCACCTCT AGGACCCCAG
    GCCACTTGTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACGAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATGAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTGGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACTGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCTGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGGGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACTCATTGCG GAAAAGAAAA
    CAGCCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCGGTT
    CCTAAACCTG GACCCGACTT GCTAAGAAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGGCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCCAG TGCTCTTCAG AATTCACTGA CACAGAACAT ACCTTTGAGA
    GGGGAAGCCG TGCACTGCCA CAATGCAAAC ACTAACTTTG TTCACAGTAA CAGTCCAGTC
    CCAAACCACC ATCTTGCAGG TTTAATAAAT CAGATTCAGG CTAGCGGGAA CTGTGGGATG
    CTCAGTCAGT CGGGCATGGC TTTAGGAAAT TCATTACATC CCAATCCACC TCAGTCAAGA
    ATTTCAACGT CCTCCACTCC AGTGATACCA AACAGCATTG TTAGCAGCTA TAATCAAACA
    AGTTCTGAAG CAGGCGGTTC AGGACCATCC TCCTCCATAG CCATAGCTGG CACCAACCAC
    CCTGCCATCA CAAAGACAAC ATCTGTTCTT CAAGATGGCG TCATAGTTAC CACCGCAGCT
    GGAAACCCAC TGCAGAGTCA GCTGCCTATT GGGAGTGATT TTCCTTATGT TGGCCAGGAG
    CACGCACTTC ATTTTCCATC CAACAGCACT TCAAACAACC ATCTTCCACA CCCCTTGAAC
    CCCAGCCTCC TCAGTTCTCT ACCTATCTCT TTGCCAGTGA ATCAACAGCA TCTCCTAAAC
    CAGAATCTAT TAAATATCCT CCAGCCTTCA GCAGGAGAAG GCAAGTCTGA GATCAACCTC
    CACCCTTTAG GTTTTCTCAA CCCGAATGTA AACGCTGCTT TAGCTTTTCT CTCCGGTGAC
    ATGGATGGGC AGGTATTGCA GCCTGTTCAC TTTCAGCTCT TAGCAGCCCT GCTTCAGAAC
    CAAGCCCAAG CAGCTGCCAT GCTTCCCCTG CCATCTTTCA ATCTGACCAT CTCAGATCTT
    TTGCAACAGC AAAATACCCC TTTACCCTCA TTAACACAGA TGACAGCCCC ACCAGACCAT
    TTGCCAAGCA ATCAGTCAGA GAACAGCCGA GCTGAGACCC TTTTAACCAG CCCCCTGGGG
    AACCCTATAC CAAGCTTTGC AGGCAGTGAC ACTACTTTTA ACCCCCTGTT CCTCCCAGCT
    GTCACTGGGG CCTCAGGATT AATGGCCTTG AATCCCCAGC TGTTGGGAGG TGTCCTGAAC
    TCAGCATCGG CCAACACCGC TAATCATCCA GAGGTTTCCA TAGCAACTTC CTCCCAGGCA
    ACCACTACCA CAACCACTAC ATCATCAGCA GTGGCAGCAC TGACTGTCTC AACACTTGGT
    GGGACAGCAG TGGTGTCAAT GGCAGAAACA TTGCTGAATA TATCTAATAA TGCTGGGAAT
    ACACCTGGTC CAGCTAAACT CAACAGTAAC TCTGTGCTGC CACAGCTACT TAACCCTCTA
    CTGGGGACAG GTCTGCTTGG TGACATGTCC TCAATAAACA ATACTTTGAA TAACCATCAA
    CTGACTCATC TGCAGTCGCT GTTAAACAAC AATCAGATGT TTCCTCCAAA TCAGCAGCAG
    CAGCAGCAGC TTCTTCAAGG GTACCAGAAC CTGCAGGCAT TTCAAGGACA GCCCACAATT
    CCTTGCCCGG CTAACAGTAA CCCCATGGCT TGTCTGTTTC AGAACTTTCA GGTGAGAATG
    CAGGAAGATG CAGCTCTCCT AAACAAAAGA ATAAGCTCTC AGCCGGGGCT CACGGCACTT
    CCTGAGAATC CAAACACTAC ACTTCCACCT TTCCAAGATA CATCTTGTGA GTTGCAACCG
    AGGATTGACC CATCTCTTGG TCAGCAGGTG AAAGATGGCC TCATTGTGGG TGGCCAAGGT
    GATGCTTCTG TAGATGCCAT TTACAAAGCA GTTGTTGATG CAGCCAGCAA AGGAATGCAG
    GTTGTCATCA CCACTGCAGT CAACAGTACA ACTCAGATCA GCCCCATTCC AGCTCTGAGT
    GCCATGAGTG CCTTCACTGC CTCGATTGGT GACCCGTTAA GTCTCCCCAG TGCTGTCAGT
    GCGGTCATTC ATGGACGAAA CATGGGCAGT GTCGATCATG ATGGTAGGCT GAGGAATGCA
    AGAGGGGCTC GGCTGCCCAG GAATCTGGAC CATGGGAAAA ACTCAAATGA AGGAGATGGG
    TTTGAATATT TCAAGTCAGC AAGTTGCCAC ACATCCAAAA AACAGTGGGA TGGGGAGCAA
    AGCCCTGGAG GGGAGCGAAA CAGGTGGAAG TGTGAGGAAT TTTTAGATCA TCCAGGCCAT
    ATCCACAGTA GTCCTTGTCA CGAAAGGTCC AACAATGTCT CTACACTGCC ATTTCTGCCT
    GGGGAACAGC ACCCGGTACT GTTACCACCA AGAAACTGTC AAGGGGATAA AATTCTGGAG
    GAAAATTTCA GGTATAATAA CTACAAAAGA ACTATGATGA GTTTTAAGGA GAGACTAGAG
    AACACTGTGG AAAGATGTGC ACACATTAAT GGGAACAGAC CTCGACAGAG TCGGGGCTTT
    GGAGAGCTGC TGAGTACCAC AAGACAAGAC CTGGTCCTAG AGGAGCAGTC TCCGAGCTCC
    TCAAATAGTT TGGAAAATTC TCTAGTCAAA GACTACATCC ATTACAATGG AGACTTTAAT
    GCCAAAAGCA TTAATGGGTG TGTGCCTAGC CCTTCAGATG CTAAAAGCAT TAGTAGTGAA
    GATGACCTAA GGAATCCAGA TTCCCCTTCT TCAAATGAGT TGATACATTA TAGGCCAAGG
    ACGTTCAACG TTGGCGACTT GGTCTGGGGC CAAATCAAAG GACTTACTTC TTGGCCTGGA
    AAATTAGTAA GAGAAGACGA CGTTCACAAT TCATGTCAAC AAAGCCCCGA GGAAGGGAAG
    GTGGAGCCCG AGAAGTTGAA GACACTAACA GAAGGTTTAG AAGCCTACAG CCGAGCCCGG
    AAAAGGAACA GAAAAAGTGG AAAGCTAAAT AACCATTTAG AAGCTGCTAT TCATGAGGCC
    ATGAGTGAAC TGGACAAAAT GTCTGGGACT GTTCACCAAA TCCCACAGGG AGACAGACAA
    ATGAGACCCC CCAAACCCAA GAGGAGGAAG ATCTCCAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.