GLI3 cDNA ORF clone, Canis lupus familiaris(Dog)

The following GLI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe19652 XM_540363.5
Latest version!
Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe294660 XM_022405177.1
Latest version!
Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe294661 XM_022405178.1
Latest version!
Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe41022 XM_014120824.1
Latest version!
Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe41023 XM_014120825.2
Latest version!
Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
OCe19652 XM_540363.4 Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe41023 XM_014120825.1 Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe19652
    Clone ID Related Accession (Same CDS sequence) XM_540363.5 , XM_540363.4
    Accession Version XM_540363.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product transcriptional activator GLI3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_540363.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
    GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG
    GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC
    TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC
    CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA
    CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT
    GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG
    AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG
    CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG
    GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC
    GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC
    CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT
    CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC
    AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA
    ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG
    CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC
    CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC
    CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGA ACAAGCCGAC GAGTGAGTCC
    GCGGTGAGTA GCACTGGTGA CCTGATGCAC AACAAGCGGT CCAAGATCAA ACCTGACGAA
    GACCTACCCA GCCCGGGGCC ACGGGGCCAG CAGGAACAGC CAGAAGGAAC AACCCTGGTA
    AAGGAAGAGG GTGACAGAGA CGAAAGCAAA CAAGAGCCCG AAGTCATCTA TGAGACCAAC
    TGCCACTGGG AAGGTTGCAC GCGGGAGTTT GACACCCAGG AGCAGCTGGT GCACCATATA
    AATAATGACC ATATTCACGG CGAGAAGAAG GAGTTCGTGT GCCGGTGGAT GGATTGCTCG
    AGGGAGCAGA AGCCCTTCAA AGCCCAGTAC ATGCTGGTGG TGCACATGAG GAGACACACC
    GGGGAGAAAC CTCACAAATG CACTTTTGAA GGTTGTACAA AGGCCTACTC GAGACTAGAA
    AACTTGAAAA CGCACTTGAG ATCTCACACT GGAGAGAAAC CGTACGTCTG TGAGCACGAA
    GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AGCACCAAAA CAGGACGCAT
    TCCAATGAGA AACCCTATGT GTGCAAAATC CCAGGCTGCA CCAAGCGCTA CACAGACCCA
    AGTTCCCTCC GGAAACATGT GAAGACCGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG
    CAGCGTGGGG ACATACATCC TCGGCCCCCC CCACCCCGAG ATTCGGGCAG CCATTCACAG
    TCCAGGTCGC CTGGCCGGCA GACTCAGGGA GCCCTTGGGG AGCAGAAGGA CCTCAGCAAC
    ACTACCTCAA AGCGGGAAGA ATGCCTCCAA GTGAAAGCTG TCAAGGCCGA GAAGCCCATG
    ACATCTCAGC CAAGCCCTGG TGGTGAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC
    AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGACG GAGGTGGCGT GGGAGACCTC
    GGGACCATCG ACGAAACCCC AATCATGGAC TCGACCATTT CCACCGCAAC CACCGCCCTC
    GCCTTGCAAG CCAGGAGGAA CCCGGCAGGG GCCAAATGGA TGGAGCATGT GAAACTAGAA
    AGGCTCAAAC AAGTGAATGG AATGCTTCCA CGACTGAACC CCGTTCTTCC CCCCAAAGCC
    CCTGCAGTCT CTCCTCTCAT AGGAAACGGC ACTCAGCCCA GCACCAGCTG CAGTTTGGGC
    GGGCCCATAA CCCTCCTCCC GAACAGGAGC GACCTCTCCG GGGTGGACAT CACCATGCTG
    AACATGCTCA ACCGGAGGGA CAGCAGCGCC AGCACCATCA GCTCCGCCTA CCTGAGCAGC
    CGCCGCTCCT CGGGCATCTC CCCCTGCTTT TCCAGCCGCC GGTCCAGCGA GGCGTCCCAG
    GCCGAGGGCC GGCCCCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC
    GCGTCGCGCA GGTCCAGCGA GGCGAGCCAG GGCGACGGGC TGCCCAGCCT GCTCAGCCTC
    ACGCCGGCGC AGCAGTACCG CCTGAAGGCC AAGTACGCGG CGGCCACGGG CGGGCCGCCC
    CCCACCCCGC TGCCCAACAT GGAGAGGATG AGCCTCAAGA CCAGGATGGC CCTGCTCGGG
    GACGCCCGGG AGCCCGCGGG GGCCCTGCCG CCCGTGCCCC CTCCGCGGCG GTGCAGCGAC
    GGCGGCGCCC ACGGCTACGG CCGCCGCCCC CTGCTGCCCC TGGACGCGCT GGGCAACGGC
    GTGCGCAGGG CCAGCGACCC GGTGCGGACG GCGTCCGACG GCCTCTCCCT GCCCCGCGTG
    CAGCGCTTCG GCAGCCTTCA CAGCTTCAAC CCGCCCGGGC CGCCTCCCAC CCTGGAGAAG
    CGGCACCTGG TGCTCCAGAA TTACACGCGG CCCGACGGCG GCCAGCCCCG CCACTTCCCG
    CCGTCCCCCT GTCCGCCGAG CATCACCGAG AACGTCACCC TGGAGGCCCT GACCATGGAC
    GCCGACGTGA GCCTCACCGA TGAGGACTTC CTGCCCGACG ACGTGGTGCA GTATCTGAAT
    TCCCAGAACC CGGCCGGGTT CGAGCAGCCC TTCCAGAGCG CCGTCTCCGA GGACAGCAAA
    GCGCCCCACG GGCCCGGCGA CTTCGACGGC CCGGGGCTGC CGGACGGTCA CGCGGGCCAC
    CCAGGCCACC CGGGCCACCC GGGCCCCGCC GGCCAGCCGT ACCGCCCGCT GGAGCAGCCG
    TGCCCCGAAG CCAGCAAAGC CGACCTGCCC ATTCAGTGGA ACGAAGTGAG CTCGGGCAGC
    GCCGACCTGT GCTCCTCCAA GCTCAAGTGT GGCCAGCGGC CGGCGGCGCA GCAGGCCCGG
    GCCTTCGGCT TCTACGGCAA CGTGGGCGCC CCCCCGCAGC CCGCCTTGCG GAGCGGGCCC
    GGCCCCGCCG GCGCCTATGC CCCCCTGGCC GAGCCCGGCC CGGCCTACGG CGGCCCCGAG
    CCCTTGGCGG GCCACGGCGC TGGAGCGGGC AGCCCCGGAG ACGCGTTCCA CGAACAGCCC
    TGCAAGGCCC CGCAGTTCGG GAGCTGCCTG GGCAGGCAGC CGGGCGCCCC CGGCCCCCTC
    GACGGCGGCT GCGCTGCGGC GGCAAAGCTC AAGAGCAGCA CGGCGCCGGG GCCCGGGGGC
    CAGCCCGACT TTGGCCTGCT GCCGGGGCCC GGCGAGTCAG CGGGCGGCGC CGGGAGCGGC
    CTGGCCACCC AAGACCTGGC AGGACAGGGG TACCTGGCCC ATCCGCTGCT CCACGACGGC
    GTGCCCCACC AAGGGGCGGG CCGCGCAGGC CAGCAGATGC TAGGGCAGGT TAGCGCTACC
    TCACACGTCA ACGTCTATCT GGGGCCCGAG GGCGGCCTGC CAGGGCCGCC GTGCGTCAGC
    GGCCAGCCCG CCGGCTTGGC GGCCACCCGG GGCTACCAGC CGTGTGCGGC CTACGGGGGC
    GGCAGGCGCC AGGCTGTGCC GAGGGGCAGC CTCGCTCTGC AGCAGGCACA GCTCAGTGAC
    CCAAGTCCGA CCTGCAGGGT GAACGGCATC AAGGTGGAGA TGCAGGGGCA GCCCCATCAG
    CTCTGTTCGA ACACGCAGAG TTACTCTGGT CAGTTCTATG ACCAAACCGT TGGCTTCAGT
    CAGCAAGACA TGAAAACTGG TTCATTCTCC ATTTCAGAAG CCAGCTGCCT GCTACAGGGG
    GCCAGCGCCG AGAACTCTGA ATTACTTTCC CCAGGTGCTA ACCAGGTGAC GAGCACGGTT
    GACAGCCTCG ACAGTCATGA CCTGGAAGGG GTGCAGATCG ATTTTGACGC CATCATAGAC
    GACGGGGACC ACGCCAGCCT GATGTCAGGA GCCCTGAGTC CCAGTATCAT CCAGAACCTC
    TCCCATAGCT CCTCCCGCCT CACGACACCC CGCGCGTCCC TCCCGCTGCC CACGCTGTCC
    GTGAGCACCA CCAACATGGC CATCGGGGAC ATGAGCTCCC TGCTGACCTC CCTGGCGGAA
    GAAAGCAAAT TCCTTGCAGT GATGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_540363.2
    CDS132..4901
    Translation

    Target ORF information:

    RefSeq Version XM_540363.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_540363.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
    GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG
    GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC
    TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC
    CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA
    CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT
    GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG
    AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG
    CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG
    GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC
    GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC
    CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT
    CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC
    AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA
    ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG
    CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC
    CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC
    CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGA ACAAGCCGAC GAGTGAGTCC
    GCGGTGAGTA GCACTGGTGA CCTGATGCAC AACAAGCGGT CCAAGATCAA ACCTGACGAA
    GACCTACCCA GCCCGGGGCC ACGGGGCCAG CAGGAACAGC CAGAAGGAAC AACCCTGGTA
    AAGGAAGAGG GTGACAGAGA CGAAAGCAAA CAAGAGCCCG AAGTCATCTA TGAGACCAAC
    TGCCACTGGG AAGGTTGCAC GCGGGAGTTT GACACCCAGG AGCAGCTGGT GCACCATATA
    AATAATGACC ATATTCACGG CGAGAAGAAG GAGTTCGTGT GCCGGTGGAT GGATTGCTCG
    AGGGAGCAGA AGCCCTTCAA AGCCCAGTAC ATGCTGGTGG TGCACATGAG GAGACACACC
    GGGGAGAAAC CTCACAAATG CACTTTTGAA GGTTGTACAA AGGCCTACTC GAGACTAGAA
    AACTTGAAAA CGCACTTGAG ATCTCACACT GGAGAGAAAC CGTACGTCTG TGAGCACGAA
    GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AGCACCAAAA CAGGACGCAT
    TCCAATGAGA AACCCTATGT GTGCAAAATC CCAGGCTGCA CCAAGCGCTA CACAGACCCA
    AGTTCCCTCC GGAAACATGT GAAGACCGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG
    CAGCGTGGGG ACATACATCC TCGGCCCCCC CCACCCCGAG ATTCGGGCAG CCATTCACAG
    TCCAGGTCGC CTGGCCGGCA GACTCAGGGA GCCCTTGGGG AGCAGAAGGA CCTCAGCAAC
    ACTACCTCAA AGCGGGAAGA ATGCCTCCAA GTGAAAGCTG TCAAGGCCGA GAAGCCCATG
    ACATCTCAGC CAAGCCCTGG TGGTGAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC
    AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGACG GAGGTGGCGT GGGAGACCTC
    GGGACCATCG ACGAAACCCC AATCATGGAC TCGACCATTT CCACCGCAAC CACCGCCCTC
    GCCTTGCAAG CCAGGAGGAA CCCGGCAGGG GCCAAATGGA TGGAGCATGT GAAACTAGAA
    AGGCTCAAAC AAGTGAATGG AATGCTTCCA CGACTGAACC CCGTTCTTCC CCCCAAAGCC
    CCTGCAGTCT CTCCTCTCAT AGGAAACGGC ACTCAGCCCA GCACCAGCTG CAGTTTGGGC
    GGGCCCATAA CCCTCCTCCC GAACAGGAGC GACCTCTCCG GGGTGGACAT CACCATGCTG
    AACATGCTCA ACCGGAGGGA CAGCAGCGCC AGCACCATCA GCTCCGCCTA CCTGAGCAGC
    CGCCGCTCCT CGGGCATCTC CCCCTGCTTT TCCAGCCGCC GGTCCAGCGA GGCGTCCCAG
    GCCGAGGGCC GGCCCCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC
    GCGTCGCGCA GGTCCAGCGA GGCGAGCCAG GGCGACGGGC TGCCCAGCCT GCTCAGCCTC
    ACGCCGGCGC AGCAGTACCG CCTGAAGGCC AAGTACGCGG CGGCCACGGG CGGGCCGCCC
    CCCACCCCGC TGCCCAACAT GGAGAGGATG AGCCTCAAGA CCAGGATGGC CCTGCTCGGG
    GACGCCCGGG AGCCCGCGGG GGCCCTGCCG CCCGTGCCCC CTCCGCGGCG GTGCAGCGAC
    GGCGGCGCCC ACGGCTACGG CCGCCGCCCC CTGCTGCCCC TGGACGCGCT GGGCAACGGC
    GTGCGCAGGG CCAGCGACCC GGTGCGGACG GCGTCCGACG GCCTCTCCCT GCCCCGCGTG
    CAGCGCTTCG GCAGCCTTCA CAGCTTCAAC CCGCCCGGGC CGCCTCCCAC CCTGGAGAAG
    CGGCACCTGG TGCTCCAGAA TTACACGCGG CCCGACGGCG GCCAGCCCCG CCACTTCCCG
    CCGTCCCCCT GTCCGCCGAG CATCACCGAG AACGTCACCC TGGAGGCCCT GACCATGGAC
    GCCGACGTGA GCCTCACCGA TGAGGACTTC CTGCCCGACG ACGTGGTGCA GTATCTGAAT
    TCCCAGAACC CGGCCGGGTT CGAGCAGCCC TTCCAGAGCG CCGTCTCCGA GGACAGCAAA
    GCGCCCCACG GGCCCGGCGA CTTCGACGGC CCGGGGCTGC CGGACGGTCA CGCGGGCCAC
    CCAGGCCACC CGGGCCACCC GGGCCCCGCC GGCCAGCCGT ACCGCCCGCT GGAGCAGCCG
    TGCCCCGAAG CCAGCAAAGC CGACCTGCCC ATTCAGTGGA ACGAAGTGAG CTCGGGCAGC
    GCCGACCTGT GCTCCTCCAA GCTCAAGTGT GGCCAGCGGC CGGCGGCGCA GCAGGCCCGG
    GCCTTCGGCT TCTACGGCAA CGTGGGCGCC CCCCCGCAGC CCGCCTTGCG GAGCGGGCCC
    GGCCCCGCCG GCGCCTATGC CCCCCTGGCC GAGCCCGGCC CGGCCTACGG CGGCCCCGAG
    CCCTTGGCGG GCCACGGCGC TGGAGCGGGC AGCCCCGGAG ACGCGTTCCA CGAACAGCCC
    TGCAAGGCCC CGCAGTTCGG GAGCTGCCTG GGCAGGCAGC CGGGCGCCCC CGGCCCCCTC
    GACGGCGGCT GCGCTGCGGC GGCAAAGCTC AAGAGCAGCA CGGCGCCGGG GCCCGGGGGC
    CAGCCCGACT TTGGCCTGCT GCCGGGGCCC GGCGAGTCAG CGGGCGGCGC CGGGAGCGGC
    CTGGCCACCC AAGACCTGGC AGGACAGGGG TACCTGGCCC ATCCGCTGCT CCACGACGGC
    GTGCCCCACC AAGGGGCGGG CCGCGCAGGC CAGCAGATGC TAGGGCAGGT TAGCGCTACC
    TCACACGTCA ACGTCTATCT GGGGCCCGAG GGCGGCCTGC CAGGGCCGCC GTGCGTCAGC
    GGCCAGCCCG CCGGCTTGGC GGCCACCCGG GGCTACCAGC CGTGTGCGGC CTACGGGGGC
    GGCAGGCGCC AGGCTGTGCC GAGGGGCAGC CTCGCTCTGC AGCAGGCACA GCTCAGTGAC
    CCAAGTCCGA CCTGCAGGGT GAACGGCATC AAGGTGGAGA TGCAGGGGCA GCCCCATCAG
    CTCTGTTCGA ACACGCAGAG TTACTCTGGT CAGTTCTATG ACCAAACCGT TGGCTTCAGT
    CAGCAAGACA TGAAAACTGG TTCATTCTCC ATTTCAGAAG CCAGCTGCCT GCTACAGGGG
    GCCAGCGCCG AGAACTCTGA ATTACTTTCC CCAGGTGCTA ACCAGGTGAC GAGCACGGTT
    GACAGCCTCG ACAGTCATGA CCTGGAAGGG GTGCAGATCG ATTTTGACGC CATCATAGAC
    GACGGGGACC ACGCCAGCCT GATGTCAGGA GCCCTGAGTC CCAGTATCAT CCAGAACCTC
    TCCCATAGCT CCTCCCGCCT CACGACACCC CGCGCGTCCC TCCCGCTGCC CACGCTGTCC
    GTGAGCACCA CCAACATGGC CATCGGGGAC ATGAGCTCCC TGCTGACCTC CCTGGCGGAA
    GAAAGCAAAT TCCTTGCAGT GATGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19652
    Clone ID Related Accession (Same CDS sequence) XM_540363.5 , XM_540363.4
    Accession Version XM_540363.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product transcriptional activator GLI3 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726091.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_540363.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
    GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG
    GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC
    TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC
    CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA
    CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT
    GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG
    AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG
    CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG
    GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC
    GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC
    CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT
    CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC
    AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA
    ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG
    CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC
    CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC
    CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGA ACAAGCCGAC GAGTGAGTCC
    GCGGTGAGTA GCACTGGTGA CCTGATGCAC AACAAGCGGT CCAAGATCAA ACCTGACGAA
    GACCTACCCA GCCCGGGGCC ACGGGGCCAG CAGGAACAGC CAGAAGGAAC AACCCTGGTA
    AAGGAAGAGG GTGACAGAGA CGAAAGCAAA CAAGAGCCCG AAGTCATCTA TGAGACCAAC
    TGCCACTGGG AAGGTTGCAC GCGGGAGTTT GACACCCAGG AGCAGCTGGT GCACCATATA
    AATAATGACC ATATTCACGG CGAGAAGAAG GAGTTCGTGT GCCGGTGGAT GGATTGCTCG
    AGGGAGCAGA AGCCCTTCAA AGCCCAGTAC ATGCTGGTGG TGCACATGAG GAGACACACC
    GGGGAGAAAC CTCACAAATG CACTTTTGAA GGTTGTACAA AGGCCTACTC GAGACTAGAA
    AACTTGAAAA CGCACTTGAG ATCTCACACT GGAGAGAAAC CGTACGTCTG TGAGCACGAA
    GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AGCACCAAAA CAGGACGCAT
    TCCAATGAGA AACCCTATGT GTGCAAAATC CCAGGCTGCA CCAAGCGCTA CACAGACCCA
    AGTTCCCTCC GGAAACATGT GAAGACCGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG
    CAGCGTGGGG ACATACATCC TCGGCCCCCC CCACCCCGAG ATTCGGGCAG CCATTCACAG
    TCCAGGTCGC CTGGCCGGCA GACTCAGGGA GCCCTTGGGG AGCAGAAGGA CCTCAGCAAC
    ACTACCTCAA AGCGGGAAGA ATGCCTCCAA GTGAAAGCTG TCAAGGCCGA GAAGCCCATG
    ACATCTCAGC CAAGCCCTGG TGGTGAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC
    AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGACG GAGGTGGCGT GGGAGACCTC
    GGGACCATCG ACGAAACCCC AATCATGGAC TCGACCATTT CCACCGCAAC CACCGCCCTC
    GCCTTGCAAG CCAGGAGGAA CCCGGCAGGG GCCAAATGGA TGGAGCATGT GAAACTAGAA
    AGGCTCAAAC AAGTGAATGG AATGCTTCCA CGACTGAACC CCGTTCTTCC CCCCAAAGCC
    CCTGCAGTCT CTCCTCTCAT AGGAAACGGC ACTCAGCCCA GCACCAGCTG CAGTTTGGGC
    GGGCCCATAA CCCTCCTCCC GAACAGGAGC GACCTCTCCG GGGTGGACAT CACCATGCTG
    AACATGCTCA ACCGGAGGGA CAGCAGCGCC AGCACCATCA GCTCCGCCTA CCTGAGCAGC
    CGCCGCTCCT CGGGCATCTC CCCCTGCTTT TCCAGCCGCC GGTCCAGCGA GGCGTCCCAG
    GCCGAGGGCC GGCCCCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC
    GCGTCGCGCA GGTCCAGCGA GGCGAGCCAG GGCGACGGGC TGCCCAGCCT GCTCAGCCTC
    ACGCCGGCGC AGCAGTACCG CCTGAAGGCC AAGTACGCGG CGGCCACGGG CGGGCCGCCC
    CCCACCCCGC TGCCCAACAT GGAGAGGATG AGCCTCAAGA CCAGGATGGC CCTGCTCGGG
    GACGCCCGGG AGCCCGCGGG GGCCCTGCCG CCCGTGCCCC CTCCGCGGCG GTGCAGCGAC
    GGCGGCGCCC ACGGCTACGG CCGCCGCCCC CTGCTGCCCC TGGACGCGCT GGGCAACGGC
    GTGCGCAGGG CCAGCGACCC GGTGCGGACG GCGTCCGACG GCCTCTCCCT GCCCCGCGTG
    CAGCGCTTCG GCAGCCTTCA CAGCTTCAAC CCGCCCGGGC CGCCTCCCAC CCTGGAGAAG
    CGGCACCTGG TGCTCCAGAA TTACACGCGG CCCGACGGCG GCCAGCCCCG CCACTTCCCG
    CCGTCCCCCT GTCCGCCGAG CATCACCGAG AACGTCACCC TGGAGGCCCT GACCATGGAC
    GCCGACGTGA GCCTCACCGA TGAGGACTTC CTGCCCGACG ACGTGGTGCA GTATCTGAAT
    TCCCAGAACC CGGCCGGGTT CGAGCAGCCC TTCCAGAGCG CCGTCTCCGA GGACAGCAAA
    GCGCCCCACG GGCCCGGCGA CTTCGACGGC CCGGGGCTGC CGGACGGTCA CGCGGGCCAC
    CCAGGCCACC CGGGCCACCC GGGCCCCGCC GGCCAGCCGT ACCGCCCGCT GGAGCAGCCG
    TGCCCCGAAG CCAGCAAAGC CGACCTGCCC ATTCAGTGGA ACGAAGTGAG CTCGGGCAGC
    GCCGACCTGT GCTCCTCCAA GCTCAAGTGT GGCCAGCGGC CGGCGGCGCA GCAGGCCCGG
    GCCTTCGGCT TCTACGGCAA CGTGGGCGCC CCCCCGCAGC CCGCCTTGCG GAGCGGGCCC
    GGCCCCGCCG GCGCCTATGC CCCCCTGGCC GAGCCCGGCC CGGCCTACGG CGGCCCCGAG
    CCCTTGGCGG GCCACGGCGC TGGAGCGGGC AGCCCCGGAG ACGCGTTCCA CGAACAGCCC
    TGCAAGGCCC CGCAGTTCGG GAGCTGCCTG GGCAGGCAGC CGGGCGCCCC CGGCCCCCTC
    GACGGCGGCT GCGCTGCGGC GGCAAAGCTC AAGAGCAGCA CGGCGCCGGG GCCCGGGGGC
    CAGCCCGACT TTGGCCTGCT GCCGGGGCCC GGCGAGTCAG CGGGCGGCGC CGGGAGCGGC
    CTGGCCACCC AAGACCTGGC AGGACAGGGG TACCTGGCCC ATCCGCTGCT CCACGACGGC
    GTGCCCCACC AAGGGGCGGG CCGCGCAGGC CAGCAGATGC TAGGGCAGGT TAGCGCTACC
    TCACACGTCA ACGTCTATCT GGGGCCCGAG GGCGGCCTGC CAGGGCCGCC GTGCGTCAGC
    GGCCAGCCCG CCGGCTTGGC GGCCACCCGG GGCTACCAGC CGTGTGCGGC CTACGGGGGC
    GGCAGGCGCC AGGCTGTGCC GAGGGGCAGC CTCGCTCTGC AGCAGGCACA GCTCAGTGAC
    CCAAGTCCGA CCTGCAGGGT GAACGGCATC AAGGTGGAGA TGCAGGGGCA GCCCCATCAG
    CTCTGTTCGA ACACGCAGAG TTACTCTGGT CAGTTCTATG ACCAAACCGT TGGCTTCAGT
    CAGCAAGACA TGAAAACTGG TTCATTCTCC ATTTCAGAAG CCAGCTGCCT GCTACAGGGG
    GCCAGCGCCG AGAACTCTGA ATTACTTTCC CCAGGTGCTA ACCAGGTGAC GAGCACGGTT
    GACAGCCTCG ACAGTCATGA CCTGGAAGGG GTGCAGATCG ATTTTGACGC CATCATAGAC
    GACGGGGACC ACGCCAGCCT GATGTCAGGA GCCCTGAGTC CCAGTATCAT CCAGAACCTC
    TCCCATAGCT CCTCCCGCCT CACGACACCC CGCGCGTCCC TCCCGCTGCC CACGCTGTCC
    GTGAGCACCA CCAACATGGC CATCGGGGAC ATGAGCTCCC TGCTGACCTC CCTGGCGGAA
    GAAAGCAAAT TCCTTGCAGT GATGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_540363.2
    CDS119..4888
    Translation

    Target ORF information:

    RefSeq Version XM_540363.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_540363.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
    GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG
    GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC
    TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC
    CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA
    CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT
    GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG
    AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG
    CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG
    GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC
    GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC
    CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT
    CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC
    AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA
    ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG
    CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC
    CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC
    CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGA ACAAGCCGAC GAGTGAGTCC
    GCGGTGAGTA GCACTGGTGA CCTGATGCAC AACAAGCGGT CCAAGATCAA ACCTGACGAA
    GACCTACCCA GCCCGGGGCC ACGGGGCCAG CAGGAACAGC CAGAAGGAAC AACCCTGGTA
    AAGGAAGAGG GTGACAGAGA CGAAAGCAAA CAAGAGCCCG AAGTCATCTA TGAGACCAAC
    TGCCACTGGG AAGGTTGCAC GCGGGAGTTT GACACCCAGG AGCAGCTGGT GCACCATATA
    AATAATGACC ATATTCACGG CGAGAAGAAG GAGTTCGTGT GCCGGTGGAT GGATTGCTCG
    AGGGAGCAGA AGCCCTTCAA AGCCCAGTAC ATGCTGGTGG TGCACATGAG GAGACACACC
    GGGGAGAAAC CTCACAAATG CACTTTTGAA GGTTGTACAA AGGCCTACTC GAGACTAGAA
    AACTTGAAAA CGCACTTGAG ATCTCACACT GGAGAGAAAC CGTACGTCTG TGAGCACGAA
    GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AGCACCAAAA CAGGACGCAT
    TCCAATGAGA AACCCTATGT GTGCAAAATC CCAGGCTGCA CCAAGCGCTA CACAGACCCA
    AGTTCCCTCC GGAAACATGT GAAGACCGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG
    CAGCGTGGGG ACATACATCC TCGGCCCCCC CCACCCCGAG ATTCGGGCAG CCATTCACAG
    TCCAGGTCGC CTGGCCGGCA GACTCAGGGA GCCCTTGGGG AGCAGAAGGA CCTCAGCAAC
    ACTACCTCAA AGCGGGAAGA ATGCCTCCAA GTGAAAGCTG TCAAGGCCGA GAAGCCCATG
    ACATCTCAGC CAAGCCCTGG TGGTGAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC
    AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGACG GAGGTGGCGT GGGAGACCTC
    GGGACCATCG ACGAAACCCC AATCATGGAC TCGACCATTT CCACCGCAAC CACCGCCCTC
    GCCTTGCAAG CCAGGAGGAA CCCGGCAGGG GCCAAATGGA TGGAGCATGT GAAACTAGAA
    AGGCTCAAAC AAGTGAATGG AATGCTTCCA CGACTGAACC CCGTTCTTCC CCCCAAAGCC
    CCTGCAGTCT CTCCTCTCAT AGGAAACGGC ACTCAGCCCA GCACCAGCTG CAGTTTGGGC
    GGGCCCATAA CCCTCCTCCC GAACAGGAGC GACCTCTCCG GGGTGGACAT CACCATGCTG
    AACATGCTCA ACCGGAGGGA CAGCAGCGCC AGCACCATCA GCTCCGCCTA CCTGAGCAGC
    CGCCGCTCCT CGGGCATCTC CCCCTGCTTT TCCAGCCGCC GGTCCAGCGA GGCGTCCCAG
    GCCGAGGGCC GGCCCCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC
    GCGTCGCGCA GGTCCAGCGA GGCGAGCCAG GGCGACGGGC TGCCCAGCCT GCTCAGCCTC
    ACGCCGGCGC AGCAGTACCG CCTGAAGGCC AAGTACGCGG CGGCCACGGG CGGGCCGCCC
    CCCACCCCGC TGCCCAACAT GGAGAGGATG AGCCTCAAGA CCAGGATGGC CCTGCTCGGG
    GACGCCCGGG AGCCCGCGGG GGCCCTGCCG CCCGTGCCCC CTCCGCGGCG GTGCAGCGAC
    GGCGGCGCCC ACGGCTACGG CCGCCGCCCC CTGCTGCCCC TGGACGCGCT GGGCAACGGC
    GTGCGCAGGG CCAGCGACCC GGTGCGGACG GCGTCCGACG GCCTCTCCCT GCCCCGCGTG
    CAGCGCTTCG GCAGCCTTCA CAGCTTCAAC CCGCCCGGGC CGCCTCCCAC CCTGGAGAAG
    CGGCACCTGG TGCTCCAGAA TTACACGCGG CCCGACGGCG GCCAGCCCCG CCACTTCCCG
    CCGTCCCCCT GTCCGCCGAG CATCACCGAG AACGTCACCC TGGAGGCCCT GACCATGGAC
    GCCGACGTGA GCCTCACCGA TGAGGACTTC CTGCCCGACG ACGTGGTGCA GTATCTGAAT
    TCCCAGAACC CGGCCGGGTT CGAGCAGCCC TTCCAGAGCG CCGTCTCCGA GGACAGCAAA
    GCGCCCCACG GGCCCGGCGA CTTCGACGGC CCGGGGCTGC CGGACGGTCA CGCGGGCCAC
    CCAGGCCACC CGGGCCACCC GGGCCCCGCC GGCCAGCCGT ACCGCCCGCT GGAGCAGCCG
    TGCCCCGAAG CCAGCAAAGC CGACCTGCCC ATTCAGTGGA ACGAAGTGAG CTCGGGCAGC
    GCCGACCTGT GCTCCTCCAA GCTCAAGTGT GGCCAGCGGC CGGCGGCGCA GCAGGCCCGG
    GCCTTCGGCT TCTACGGCAA CGTGGGCGCC CCCCCGCAGC CCGCCTTGCG GAGCGGGCCC
    GGCCCCGCCG GCGCCTATGC CCCCCTGGCC GAGCCCGGCC CGGCCTACGG CGGCCCCGAG
    CCCTTGGCGG GCCACGGCGC TGGAGCGGGC AGCCCCGGAG ACGCGTTCCA CGAACAGCCC
    TGCAAGGCCC CGCAGTTCGG GAGCTGCCTG GGCAGGCAGC CGGGCGCCCC CGGCCCCCTC
    GACGGCGGCT GCGCTGCGGC GGCAAAGCTC AAGAGCAGCA CGGCGCCGGG GCCCGGGGGC
    CAGCCCGACT TTGGCCTGCT GCCGGGGCCC GGCGAGTCAG CGGGCGGCGC CGGGAGCGGC
    CTGGCCACCC AAGACCTGGC AGGACAGGGG TACCTGGCCC ATCCGCTGCT CCACGACGGC
    GTGCCCCACC AAGGGGCGGG CCGCGCAGGC CAGCAGATGC TAGGGCAGGT TAGCGCTACC
    TCACACGTCA ACGTCTATCT GGGGCCCGAG GGCGGCCTGC CAGGGCCGCC GTGCGTCAGC
    GGCCAGCCCG CCGGCTTGGC GGCCACCCGG GGCTACCAGC CGTGTGCGGC CTACGGGGGC
    GGCAGGCGCC AGGCTGTGCC GAGGGGCAGC CTCGCTCTGC AGCAGGCACA GCTCAGTGAC
    CCAAGTCCGA CCTGCAGGGT GAACGGCATC AAGGTGGAGA TGCAGGGGCA GCCCCATCAG
    CTCTGTTCGA ACACGCAGAG TTACTCTGGT CAGTTCTATG ACCAAACCGT TGGCTTCAGT
    CAGCAAGACA TGAAAACTGG TTCATTCTCC ATTTCAGAAG CCAGCTGCCT GCTACAGGGG
    GCCAGCGCCG AGAACTCTGA ATTACTTTCC CCAGGTGCTA ACCAGGTGAC GAGCACGGTT
    GACAGCCTCG ACAGTCATGA CCTGGAAGGG GTGCAGATCG ATTTTGACGC CATCATAGAC
    GACGGGGACC ACGCCAGCCT GATGTCAGGA GCCCTGAGTC CCAGTATCAT CCAGAACCTC
    TCCCATAGCT CCTCCCGCCT CACGACACCC CGCGCGTCCC TCCCGCTGCC CACGCTGTCC
    GTGAGCACCA CCAACATGGC CATCGGGGAC ATGAGCTCCC TGCTGACCTC CCTGGCGGAA
    GAAAGCAAAT TCCTTGCAGT GATGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294660
    Clone ID Related Accession (Same CDS sequence) XM_022405177.1
    Accession Version XM_022405177.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGAAAAG ACCGAAGAAA AATGATGGGA GAATATTCTA CTAGGTATAG ACTGCCAACC 
    TGTTACCCAG CTCCGTGCAG GACCACAGAT CTAGTCATTC CATGGCTGCA TCCTAGAGAT
    TGTCAAGAGG ATGAAAGTCC TGGACAGACC TATCACAGAG AGAGAAGAAA CGCAGTCACT
    ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC
    GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG
    CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG
    CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA
    AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT
    TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC
    CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG
    GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG
    ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT
    GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC
    AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC
    GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT
    CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC
    AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT
    GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT
    GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG
    ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC
    CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT
    TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA
    CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA
    CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG
    CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG
    ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA
    GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT
    TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG
    CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC
    CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG
    GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC
    ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG
    AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC
    CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC
    AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC
    ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC
    CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG
    GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC
    TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG
    CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC
    GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC
    CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG
    TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG
    GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG
    CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC
    CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC
    CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG
    ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG
    TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG
    GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC
    GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG
    GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC
    TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG
    CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG
    AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC
    GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC
    GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC
    GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG
    CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC
    GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC
    CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT
    AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG
    TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC
    TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG
    CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG
    CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT
    GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG
    CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG
    AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC
    ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC
    CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC
    ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC
    CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260885.1
    CDS110..4888
    Translation

    Target ORF information:

    RefSeq Version XM_022405177.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022405177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGAAAAG ACCGAAGAAA AATGATGGGA GAATATTCTA CTAGGTATAG ACTGCCAACC 
    TGTTACCCAG CTCCGTGCAG GACCACAGAT CTAGTCATTC CATGGCTGCA TCCTAGAGAT
    TGTCAAGAGG ATGAAAGTCC TGGACAGACC TATCACAGAG AGAGAAGAAA CGCAGTCACT
    ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC
    GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG
    CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG
    CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA
    AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT
    TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC
    CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG
    GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG
    ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT
    GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC
    AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC
    GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT
    CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC
    AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT
    GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT
    GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG
    ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC
    CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT
    TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA
    CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA
    CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG
    CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG
    ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA
    GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT
    TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG
    CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC
    CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG
    GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC
    ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG
    AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC
    CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC
    AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC
    ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC
    CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG
    GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC
    TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG
    CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC
    GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC
    CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG
    TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG
    GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG
    CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC
    CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC
    CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG
    ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG
    TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG
    GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC
    GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG
    GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC
    TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG
    CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG
    AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC
    GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC
    GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC
    GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG
    CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC
    GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC
    CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT
    AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG
    TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC
    TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG
    CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG
    CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT
    GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG
    CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG
    AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC
    ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC
    CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC
    ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC
    CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294661
    Clone ID Related Accession (Same CDS sequence) XM_022405178.1
    Accession Version XM_022405178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product transcriptional activator GLI3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGAAAAG ACCGAAGAAA AATGATGGGA GAATATTCTA CTAGGTATAG ACTGCCAACC 
    TGTTACCCAG CTCCGTGCAG GACCACAGAT CTAGTCATTC CATGGCTGCA TCCTAGAGAT
    TGTCAAGAGG ATGAAAGTCC TGGACAGACC TATCACAGAG AGAGAAGAAA CGCAGTCACT
    ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC
    GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG
    CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG
    CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA
    AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT
    TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC
    CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG
    GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG
    ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT
    GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC
    AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC
    GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT
    CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC
    AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT
    GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT
    GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG
    ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC
    CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT
    TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA
    CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA
    CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG
    CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG
    ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA
    GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT
    TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG
    CCCATGACAT CTCAGCCAAG CCCTGGTGGT GAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGACGGAGG TGGCGTGGGA
    GACCTCGGGA CCATCGACGA AACCCCAATC ATGGACTCGA CCATTTCCAC CGCAACCACC
    GCCCTCGCCT TGCAAGCCAG GAGGAACCCG GCAGGGGCCA AATGGATGGA GCATGTGAAA
    CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCGT TCTTCCCCCC
    AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACTC AGCCCAGCAC CAGCTGCAGT
    TTGGGCGGGC CCATAACCCT CCTCCCGAAC AGGAGCGACC TCTCCGGGGT GGACATCACC
    ATGCTGAACA TGCTCAACCG GAGGGACAGC AGCGCCAGCA CCATCAGCTC CGCCTACCTG
    AGCAGCCGCC GCTCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCCGGTC CAGCGAGGCG
    TCCCAGGCCG AGGGCCGGCC CCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC
    ACCGACGCGT CGCGCAGGTC CAGCGAGGCG AGCCAGGGCG ACGGGCTGCC CAGCCTGCTC
    AGCCTCACGC CGGCGCAGCA GTACCGCCTG AAGGCCAAGT ACGCGGCGGC CACGGGCGGG
    CCGCCCCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCAG GATGGCCCTG
    CTCGGGGACG CCCGGGAGCC CGCGGGGGCC CTGCCGCCCG TGCCCCCTCC GCGGCGGTGC
    AGCGACGGCG GCGCCCACGG CTACGGCCGC CGCCCCCTGC TGCCCCTGGA CGCGCTGGGC
    AACGGCGTGC GCAGGGCCAG CGACCCGGTG CGGACGGCGT CCGACGGCCT CTCCCTGCCC
    CGCGTGCAGC GCTTCGGCAG CCTTCACAGC TTCAACCCGC CCGGGCCGCC TCCCACCCTG
    GAGAAGCGGC ACCTGGTGCT CCAGAATTAC ACGCGGCCCG ACGGCGGCCA GCCCCGCCAC
    TTCCCGCCGT CCCCCTGTCC GCCGAGCATC ACCGAGAACG TCACCCTGGA GGCCCTGACC
    ATGGACGCCG ACGTGAGCCT CACCGATGAG GACTTCCTGC CCGACGACGT GGTGCAGTAT
    CTGAATTCCC AGAACCCGGC CGGGTTCGAG CAGCCCTTCC AGAGCGCCGT CTCCGAGGAC
    AGCAAAGCGC CCCACGGGCC CGGCGACTTC GACGGCCCGG GGCTGCCGGA CGGTCACGCG
    GGCCACCCAG GCCACCCGGG CCACCCGGGC CCCGCCGGCC AGCCGTACCG CCCGCTGGAG
    CAGCCGTGCC CCGAAGCCAG CAAAGCCGAC CTGCCCATTC AGTGGAACGA AGTGAGCTCG
    GGCAGCGCCG ACCTGTGCTC CTCCAAGCTC AAGTGTGGCC AGCGGCCGGC GGCGCAGCAG
    GCCCGGGCCT TCGGCTTCTA CGGCAACGTG GGCGCCCCCC CGCAGCCCGC CTTGCGGAGC
    GGGCCCGGCC CCGCCGGCGC CTATGCCCCC CTGGCCGAGC CCGGCCCGGC CTACGGCGGC
    CCCGAGCCCT TGGCGGGCCA CGGCGCTGGA GCGGGCAGCC CCGGAGACGC GTTCCACGAA
    CAGCCCTGCA AGGCCCCGCA GTTCGGGAGC TGCCTGGGCA GGCAGCCGGG CGCCCCCGGC
    CCCCTCGACG GCGGCTGCGC TGCGGCGGCA AAGCTCAAGA GCAGCACGGC GCCGGGGCCC
    GGGGGCCAGC CCGACTTTGG CCTGCTGCCG GGGCCCGGCG AGTCAGCGGG CGGCGCCGGG
    AGCGGCCTGG CCACCCAAGA CCTGGCAGGA CAGGGGTACC TGGCCCATCC GCTGCTCCAC
    GACGGCGTGC CCCACCAAGG GGCGGGCCGC GCAGGCCAGC AGATGCTAGG GCAGGTTAGC
    GCTACCTCAC ACGTCAACGT CTATCTGGGG CCCGAGGGCG GCCTGCCAGG GCCGCCGTGC
    GTCAGCGGCC AGCCCGCCGG CTTGGCGGCC ACCCGGGGCT ACCAGCCGTG TGCGGCCTAC
    GGGGGCGGCA GGCGCCAGGC TGTGCCGAGG GGCAGCCTCG CTCTGCAGCA GGCACAGCTC
    AGTGACCCAA GTCCGACCTG CAGGGTGAAC GGCATCAAGG TGGAGATGCA GGGGCAGCCC
    CATCAGCTCT GTTCGAACAC GCAGAGTTAC TCTGGTCAGT TCTATGACCA AACCGTTGGC
    TTCAGTCAGC AAGACATGAA AACTGGTTCA TTCTCCATTT CAGAAGCCAG CTGCCTGCTA
    CAGGGGGCCA GCGCCGAGAA CTCTGAATTA CTTTCCCCAG GTGCTAACCA GGTGACGAGC
    ACGGTTGACA GCCTCGACAG TCATGACCTG GAAGGGGTGC AGATCGATTT TGACGCCATC
    ATAGACGACG GGGACCACGC CAGCCTGATG TCAGGAGCCC TGAGTCCCAG TATCATCCAG
    AACCTCTCCC ATAGCTCCTC CCGCCTCACG ACACCCCGCG CGTCCCTCCC GCTGCCCACG
    CTGTCCGTGA GCACCACCAA CATGGCCATC GGGGACATGA GCTCCCTGCT GACCTCCCTG
    GCGGAAGAAA GCAAATTCCT TGCAGTGATG CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260886.1
    CDS110..4885
    Translation

    Target ORF information:

    RefSeq Version XM_022405178.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022405178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGAAAAG ACCGAAGAAA AATGATGGGA GAATATTCTA CTAGGTATAG ACTGCCAACC 
    TGTTACCCAG CTCCGTGCAG GACCACAGAT CTAGTCATTC CATGGCTGCA TCCTAGAGAT
    TGTCAAGAGG ATGAAAGTCC TGGACAGACC TATCACAGAG AGAGAAGAAA CGCAGTCACT
    ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC
    GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG
    CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG
    CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA
    AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT
    TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC
    CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG
    GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG
    ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT
    GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC
    AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC
    GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT
    CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC
    AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT
    GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT
    GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG
    ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC
    CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT
    TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA
    CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA
    CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG
    CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG
    ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA
    GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT
    TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG
    CCCATGACAT CTCAGCCAAG CCCTGGTGGT GAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGACGGAGG TGGCGTGGGA
    GACCTCGGGA CCATCGACGA AACCCCAATC ATGGACTCGA CCATTTCCAC CGCAACCACC
    GCCCTCGCCT TGCAAGCCAG GAGGAACCCG GCAGGGGCCA AATGGATGGA GCATGTGAAA
    CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCGT TCTTCCCCCC
    AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACTC AGCCCAGCAC CAGCTGCAGT
    TTGGGCGGGC CCATAACCCT CCTCCCGAAC AGGAGCGACC TCTCCGGGGT GGACATCACC
    ATGCTGAACA TGCTCAACCG GAGGGACAGC AGCGCCAGCA CCATCAGCTC CGCCTACCTG
    AGCAGCCGCC GCTCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCCGGTC CAGCGAGGCG
    TCCCAGGCCG AGGGCCGGCC CCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC
    ACCGACGCGT CGCGCAGGTC CAGCGAGGCG AGCCAGGGCG ACGGGCTGCC CAGCCTGCTC
    AGCCTCACGC CGGCGCAGCA GTACCGCCTG AAGGCCAAGT ACGCGGCGGC CACGGGCGGG
    CCGCCCCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCAG GATGGCCCTG
    CTCGGGGACG CCCGGGAGCC CGCGGGGGCC CTGCCGCCCG TGCCCCCTCC GCGGCGGTGC
    AGCGACGGCG GCGCCCACGG CTACGGCCGC CGCCCCCTGC TGCCCCTGGA CGCGCTGGGC
    AACGGCGTGC GCAGGGCCAG CGACCCGGTG CGGACGGCGT CCGACGGCCT CTCCCTGCCC
    CGCGTGCAGC GCTTCGGCAG CCTTCACAGC TTCAACCCGC CCGGGCCGCC TCCCACCCTG
    GAGAAGCGGC ACCTGGTGCT CCAGAATTAC ACGCGGCCCG ACGGCGGCCA GCCCCGCCAC
    TTCCCGCCGT CCCCCTGTCC GCCGAGCATC ACCGAGAACG TCACCCTGGA GGCCCTGACC
    ATGGACGCCG ACGTGAGCCT CACCGATGAG GACTTCCTGC CCGACGACGT GGTGCAGTAT
    CTGAATTCCC AGAACCCGGC CGGGTTCGAG CAGCCCTTCC AGAGCGCCGT CTCCGAGGAC
    AGCAAAGCGC CCCACGGGCC CGGCGACTTC GACGGCCCGG GGCTGCCGGA CGGTCACGCG
    GGCCACCCAG GCCACCCGGG CCACCCGGGC CCCGCCGGCC AGCCGTACCG CCCGCTGGAG
    CAGCCGTGCC CCGAAGCCAG CAAAGCCGAC CTGCCCATTC AGTGGAACGA AGTGAGCTCG
    GGCAGCGCCG ACCTGTGCTC CTCCAAGCTC AAGTGTGGCC AGCGGCCGGC GGCGCAGCAG
    GCCCGGGCCT TCGGCTTCTA CGGCAACGTG GGCGCCCCCC CGCAGCCCGC CTTGCGGAGC
    GGGCCCGGCC CCGCCGGCGC CTATGCCCCC CTGGCCGAGC CCGGCCCGGC CTACGGCGGC
    CCCGAGCCCT TGGCGGGCCA CGGCGCTGGA GCGGGCAGCC CCGGAGACGC GTTCCACGAA
    CAGCCCTGCA AGGCCCCGCA GTTCGGGAGC TGCCTGGGCA GGCAGCCGGG CGCCCCCGGC
    CCCCTCGACG GCGGCTGCGC TGCGGCGGCA AAGCTCAAGA GCAGCACGGC GCCGGGGCCC
    GGGGGCCAGC CCGACTTTGG CCTGCTGCCG GGGCCCGGCG AGTCAGCGGG CGGCGCCGGG
    AGCGGCCTGG CCACCCAAGA CCTGGCAGGA CAGGGGTACC TGGCCCATCC GCTGCTCCAC
    GACGGCGTGC CCCACCAAGG GGCGGGCCGC GCAGGCCAGC AGATGCTAGG GCAGGTTAGC
    GCTACCTCAC ACGTCAACGT CTATCTGGGG CCCGAGGGCG GCCTGCCAGG GCCGCCGTGC
    GTCAGCGGCC AGCCCGCCGG CTTGGCGGCC ACCCGGGGCT ACCAGCCGTG TGCGGCCTAC
    GGGGGCGGCA GGCGCCAGGC TGTGCCGAGG GGCAGCCTCG CTCTGCAGCA GGCACAGCTC
    AGTGACCCAA GTCCGACCTG CAGGGTGAAC GGCATCAAGG TGGAGATGCA GGGGCAGCCC
    CATCAGCTCT GTTCGAACAC GCAGAGTTAC TCTGGTCAGT TCTATGACCA AACCGTTGGC
    TTCAGTCAGC AAGACATGAA AACTGGTTCA TTCTCCATTT CAGAAGCCAG CTGCCTGCTA
    CAGGGGGCCA GCGCCGAGAA CTCTGAATTA CTTTCCCCAG GTGCTAACCA GGTGACGAGC
    ACGGTTGACA GCCTCGACAG TCATGACCTG GAAGGGGTGC AGATCGATTT TGACGCCATC
    ATAGACGACG GGGACCACGC CAGCCTGATG TCAGGAGCCC TGAGTCCCAG TATCATCCAG
    AACCTCTCCC ATAGCTCCTC CCGCCTCACG ACACCCCGCG CGTCCCTCCC GCTGCCCACG
    CTGTCCGTGA GCACCACCAA CATGGCCATC GGGGACATGA GCTCCCTGCT GACCTCCCTG
    GCGGAAGAAA GCAAATTCCT TGCAGTGATG CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41022
    Clone ID Related Accession (Same CDS sequence) XM_014120824.1
    Accession Version XM_014120824.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product transcriptional activator GLI3 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726091.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
    GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG
    GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC
    TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC
    CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA
    CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT
    GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG
    AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG
    CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG
    GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC
    GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC
    CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT
    CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC
    AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA
    ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG
    CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC
    CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC
    CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGC AGAACAAGCC GACGAGTGAG
    TCCGCGGTGA GTAGCACTGG TGACCTGATG CACAACAAGC GGTCCAAGAT CAAACCTGAC
    GAAGACCTAC CCAGCCCGGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG
    GTAAAGGAAG AGGGTGACAG AGACGAAAGC AAACAAGAGC CCGAAGTCAT CTATGAGACC
    AACTGCCACT GGGAAGGTTG CACGCGGGAG TTTGACACCC AGGAGCAGCT GGTGCACCAT
    ATAAATAATG ACCATATTCA CGGCGAGAAG AAGGAGTTCG TGTGCCGGTG GATGGATTGC
    TCGAGGGAGC AGAAGCCCTT CAAAGCCCAG TACATGCTGG TGGTGCACAT GAGGAGACAC
    ACCGGGGAGA AACCTCACAA ATGCACTTTT GAAGGTTGTA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACGCACTT GAGATCTCAC ACTGGAGAGA AACCGTACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAGCACCA AAACAGGACG
    CATTCCAATG AGAAACCCTA TGTGTGCAAA ATCCCAGGCT GCACCAAGCG CTACACAGAC
    CCAAGTTCCC TCCGGAAACA TGTGAAGACC GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGTG GGGACATACA TCCTCGGCCC CCCCCACCCC GAGATTCGGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG GCAGACTCAG GGAGCCCTTG GGGAGCAGAA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAG CTGTCAAGGC CGAGAAGCCC
    ATGCAGACAT CTCAGCCAAG CCCTGGTGGT GAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGACGGAGG TGGCGTGGGA
    GACCTCGGGA CCATCGACGA AACCCCAATC ATGGACTCGA CCATTTCCAC CGCAACCACC
    GCCCTCGCCT TGCAAGCCAG GAGGAACCCG GCAGGGGCCA AATGGATGGA GCATGTGAAA
    CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCGT TCTTCCCCCC
    AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACTC AGCCCAGCAC CAGCTGCAGT
    TTGGGCGGGC CCATAACCCT CCTCCCGAAC AGGAGCGACC TCTCCGGGGT GGACATCACC
    ATGCTGAACA TGCTCAACCG GAGGGACAGC AGCGCCAGCA CCATCAGCTC CGCCTACCTG
    AGCAGCCGCC GCTCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCCGGTC CAGCGAGGCG
    TCCCAGGCCG AGGGCCGGCC CCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC
    ACCGACGCGT CGCGCAGGTC CAGCGAGGCG AGCCAGGGCG ACGGGCTGCC CAGCCTGCTC
    AGCCTCACGC CGGCGCAGCA GTACCGCCTG AAGGCCAAGT ACGCGGCGGC CACGGGCGGG
    CCGCCCCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCAG GATGGCCCTG
    CTCGGGGACG CCCGGGAGCC CGCGGGGGCC CTGCCGCCCG TGCCCCCTCC GCGGCGGTGC
    AGCGACGGCG GCGCCCACGG CTACGGCCGC CGCCCCCTGC TGCCCCTGGA CGCGCTGGGC
    AACGGCGTGC GCAGGGCCAG CGACCCGGTG CGGACGGCGT CCGACGGCCT CTCCCTGCCC
    CGCGTGCAGC GCTTCGGCAG CCTTCACAGC TTCAACCCGC CCGGGCCGCC TCCCACCCTG
    GAGAAGCGGC ACCTGGTGCT CCAGAATTAC ACGCGGCCCG ACGGCGGCCA GCCCCGCCAC
    TTCCCGCCGT CCCCCTGTCC GCCGAGCATC ACCGAGAACG TCACCCTGGA GGCCCTGACC
    ATGGACGCCG ACGTGAGCCT CACCGATGAG GACTTCCTGC CCGACGACGT GGTGCAGTAT
    CTGAATTCCC AGAACCCGGC CGGGTTCGAG CAGCCCTTCC AGAGCGCCGT CTCCGAGGAC
    AGCAAAGCGC CCCACGGGCC CGGCGACTTC GACGGCCCGG GGCTGCCGGA CGGTCACGCG
    GGCCACCCAG GCCACCCGGG CCACCCGGGC CCCGCCGGCC AGCCGTACCG CCCGCTGGAG
    CAGCCGTGCC CCGAAGCCAG CAAAGCCGAC CTGCCCATTC AGTGGAACGA AGTGAGCTCG
    GGCAGCGCCG ACCTGTGCTC CTCCAAGCTC AAGTGTGGCC AGCGGCCGGC GGCGCAGCAG
    GCCCGGGCCT TCGGCTTCTA CGGCAACGTG GGCGCCCCCC CGCAGCCCGC CTTGCGGAGC
    GGGCCCGGCC CCGCCGGCGC CTATGCCCCC CTGGCCGAGC CCGGCCCGGC CTACGGCGGC
    CCCGAGCCCT TGGCGGGCCA CGGCGCTGGA GCGGGCAGCC CCGGAGACGC GTTCCACGAA
    CAGCCCTGCA AGGCCCCGCA GTTCGGGAGC TGCCTGGGCA GGCAGCCGGG CGCCCCCGGC
    CCCCTCGACG GCGGCTGCGC TGCGGCGGCA AAGCTCAAGA GCAGCACGGC GCCGGGGCCC
    GGGGGCCAGC CCGACTTTGG CCTGCTGCCG GGGCCCGGCG AGTCAGCGGG CGGCGCCGGG
    AGCGGCCTGG CCACCCAAGA CCTGGCAGGA CAGGGGTACC TGGCCCATCC GCTGCTCCAC
    GACGGCGTGC CCCACCAAGG GGCGGGCCGC GCAGGCCAGC AGATGCTAGG GCAGGTTAGC
    GCTACCTCAC ACGTCAACGT CTATCTGGGG CCCGAGGGCG GCCTGCCAGG GCCGCCGTGC
    GTCAGCGGCC AGCCCGCCGG CTTGGCGGCC ACCCGGGGCT ACCAGCCGTG TGCGGCCTAC
    GGGGGCGGCA GGCGCCAGGC TGTGCCGAGG GGCAGCCTCG CTCTGCAGCA GGCACAGCTC
    AGTGACCCAA GTCCGACCTG CAGGGTGAAC GGCATCAAGG TGGAGATGCA GGGGCAGCCC
    CATCAGCTCT GTTCGAACAC GCAGAGTTAC TCTGGTCAGT TCTATGACCA AACCGTTGGC
    TTCAGTCAGC AAGACATGAA AACTGGTTCA TTCTCCATTT CAGAAGCCAG CTGCCTGCTA
    CAGGGGGCCA GCGCCGAGAA CTCTGAATTA CTTTCCCCAG GTGCTAACCA GGTGACGAGC
    ACGGTTGACA GCCTCGACAG TCATGACCTG GAAGGGGTGC AGATCGATTT TGACGCCATC
    ATAGACGACG GGGACCACGC CAGCCTGATG TCAGGAGCCC TGAGTCCCAG TATCATCCAG
    AACCTCTCCC ATAGCTCCTC CCGCCTCACG ACACCCCGCG CGTCCCTCCC GCTGCCCACG
    CTGTCCGTGA GCACCACCAA CATGGCCATC GGGGACATGA GCTCCCTGCT GACCTCCCTG
    GCGGAAGAAA GCAAATTCCT TGCAGTGATG CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976299.1
    CDS119..4894
    Translation

    Target ORF information:

    RefSeq Version XM_014120824.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
    GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG
    CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG
    GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC
    TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC
    CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA
    CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT
    GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG
    AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC
    ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG
    CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG
    GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC
    GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC
    CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT
    CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC
    AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA
    ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG
    CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC
    CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC
    CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGC AGAACAAGCC GACGAGTGAG
    TCCGCGGTGA GTAGCACTGG TGACCTGATG CACAACAAGC GGTCCAAGAT CAAACCTGAC
    GAAGACCTAC CCAGCCCGGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG
    GTAAAGGAAG AGGGTGACAG AGACGAAAGC AAACAAGAGC CCGAAGTCAT CTATGAGACC
    AACTGCCACT GGGAAGGTTG CACGCGGGAG TTTGACACCC AGGAGCAGCT GGTGCACCAT
    ATAAATAATG ACCATATTCA CGGCGAGAAG AAGGAGTTCG TGTGCCGGTG GATGGATTGC
    TCGAGGGAGC AGAAGCCCTT CAAAGCCCAG TACATGCTGG TGGTGCACAT GAGGAGACAC
    ACCGGGGAGA AACCTCACAA ATGCACTTTT GAAGGTTGTA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACGCACTT GAGATCTCAC ACTGGAGAGA AACCGTACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAGCACCA AAACAGGACG
    CATTCCAATG AGAAACCCTA TGTGTGCAAA ATCCCAGGCT GCACCAAGCG CTACACAGAC
    CCAAGTTCCC TCCGGAAACA TGTGAAGACC GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGTG GGGACATACA TCCTCGGCCC CCCCCACCCC GAGATTCGGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG GCAGACTCAG GGAGCCCTTG GGGAGCAGAA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAG CTGTCAAGGC CGAGAAGCCC
    ATGCAGACAT CTCAGCCAAG CCCTGGTGGT GAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGACGGAGG TGGCGTGGGA
    GACCTCGGGA CCATCGACGA AACCCCAATC ATGGACTCGA CCATTTCCAC CGCAACCACC
    GCCCTCGCCT TGCAAGCCAG GAGGAACCCG GCAGGGGCCA AATGGATGGA GCATGTGAAA
    CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCGT TCTTCCCCCC
    AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACTC AGCCCAGCAC CAGCTGCAGT
    TTGGGCGGGC CCATAACCCT CCTCCCGAAC AGGAGCGACC TCTCCGGGGT GGACATCACC
    ATGCTGAACA TGCTCAACCG GAGGGACAGC AGCGCCAGCA CCATCAGCTC CGCCTACCTG
    AGCAGCCGCC GCTCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCCGGTC CAGCGAGGCG
    TCCCAGGCCG AGGGCCGGCC CCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC
    ACCGACGCGT CGCGCAGGTC CAGCGAGGCG AGCCAGGGCG ACGGGCTGCC CAGCCTGCTC
    AGCCTCACGC CGGCGCAGCA GTACCGCCTG AAGGCCAAGT ACGCGGCGGC CACGGGCGGG
    CCGCCCCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCAG GATGGCCCTG
    CTCGGGGACG CCCGGGAGCC CGCGGGGGCC CTGCCGCCCG TGCCCCCTCC GCGGCGGTGC
    AGCGACGGCG GCGCCCACGG CTACGGCCGC CGCCCCCTGC TGCCCCTGGA CGCGCTGGGC
    AACGGCGTGC GCAGGGCCAG CGACCCGGTG CGGACGGCGT CCGACGGCCT CTCCCTGCCC
    CGCGTGCAGC GCTTCGGCAG CCTTCACAGC TTCAACCCGC CCGGGCCGCC TCCCACCCTG
    GAGAAGCGGC ACCTGGTGCT CCAGAATTAC ACGCGGCCCG ACGGCGGCCA GCCCCGCCAC
    TTCCCGCCGT CCCCCTGTCC GCCGAGCATC ACCGAGAACG TCACCCTGGA GGCCCTGACC
    ATGGACGCCG ACGTGAGCCT CACCGATGAG GACTTCCTGC CCGACGACGT GGTGCAGTAT
    CTGAATTCCC AGAACCCGGC CGGGTTCGAG CAGCCCTTCC AGAGCGCCGT CTCCGAGGAC
    AGCAAAGCGC CCCACGGGCC CGGCGACTTC GACGGCCCGG GGCTGCCGGA CGGTCACGCG
    GGCCACCCAG GCCACCCGGG CCACCCGGGC CCCGCCGGCC AGCCGTACCG CCCGCTGGAG
    CAGCCGTGCC CCGAAGCCAG CAAAGCCGAC CTGCCCATTC AGTGGAACGA AGTGAGCTCG
    GGCAGCGCCG ACCTGTGCTC CTCCAAGCTC AAGTGTGGCC AGCGGCCGGC GGCGCAGCAG
    GCCCGGGCCT TCGGCTTCTA CGGCAACGTG GGCGCCCCCC CGCAGCCCGC CTTGCGGAGC
    GGGCCCGGCC CCGCCGGCGC CTATGCCCCC CTGGCCGAGC CCGGCCCGGC CTACGGCGGC
    CCCGAGCCCT TGGCGGGCCA CGGCGCTGGA GCGGGCAGCC CCGGAGACGC GTTCCACGAA
    CAGCCCTGCA AGGCCCCGCA GTTCGGGAGC TGCCTGGGCA GGCAGCCGGG CGCCCCCGGC
    CCCCTCGACG GCGGCTGCGC TGCGGCGGCA AAGCTCAAGA GCAGCACGGC GCCGGGGCCC
    GGGGGCCAGC CCGACTTTGG CCTGCTGCCG GGGCCCGGCG AGTCAGCGGG CGGCGCCGGG
    AGCGGCCTGG CCACCCAAGA CCTGGCAGGA CAGGGGTACC TGGCCCATCC GCTGCTCCAC
    GACGGCGTGC CCCACCAAGG GGCGGGCCGC GCAGGCCAGC AGATGCTAGG GCAGGTTAGC
    GCTACCTCAC ACGTCAACGT CTATCTGGGG CCCGAGGGCG GCCTGCCAGG GCCGCCGTGC
    GTCAGCGGCC AGCCCGCCGG CTTGGCGGCC ACCCGGGGCT ACCAGCCGTG TGCGGCCTAC
    GGGGGCGGCA GGCGCCAGGC TGTGCCGAGG GGCAGCCTCG CTCTGCAGCA GGCACAGCTC
    AGTGACCCAA GTCCGACCTG CAGGGTGAAC GGCATCAAGG TGGAGATGCA GGGGCAGCCC
    CATCAGCTCT GTTCGAACAC GCAGAGTTAC TCTGGTCAGT TCTATGACCA AACCGTTGGC
    TTCAGTCAGC AAGACATGAA AACTGGTTCA TTCTCCATTT CAGAAGCCAG CTGCCTGCTA
    CAGGGGGCCA GCGCCGAGAA CTCTGAATTA CTTTCCCCAG GTGCTAACCA GGTGACGAGC
    ACGGTTGACA GCCTCGACAG TCATGACCTG GAAGGGGTGC AGATCGATTT TGACGCCATC
    ATAGACGACG GGGACCACGC CAGCCTGATG TCAGGAGCCC TGAGTCCCAG TATCATCCAG
    AACCTCTCCC ATAGCTCCTC CCGCCTCACG ACACCCCGCG CGTCCCTCCC GCTGCCCACG
    CTGTCCGTGA GCACCACCAA CATGGCCATC GGGGACATGA GCTCCCTGCT GACCTCCCTG
    GCGGAAGAAA GCAAATTCCT TGCAGTGATG CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41023
    Clone ID Related Accession (Same CDS sequence) XM_014120825.2 , XM_014120825.1
    Accession Version XM_014120825.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product transcriptional activator GLI3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
    GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG
    CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG
    CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA
    AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT
    TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC
    CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG
    GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG
    ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT
    GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC
    AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC
    GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT
    CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC
    AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT
    GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT
    GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG
    ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC
    CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT
    TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA
    CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA
    CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG
    CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG
    ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA
    GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT
    TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG
    CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC
    CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG
    GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC
    ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG
    AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC
    CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC
    AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC
    ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC
    CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG
    GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC
    TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG
    CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC
    GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC
    CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG
    TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG
    GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG
    CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC
    CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC
    CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG
    ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG
    TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG
    GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC
    GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG
    GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC
    TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG
    CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG
    AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC
    GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC
    GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC
    GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG
    CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC
    GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC
    CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT
    AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG
    TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC
    TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG
    CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG
    CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT
    GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG
    CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG
    AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC
    ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC
    CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC
    ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC
    CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976300.1
    CDS268..4866
    Translation

    Target ORF information:

    RefSeq Version XM_014120825.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120825.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
    GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG
    CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG
    CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA
    AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT
    TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC
    CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG
    GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG
    ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT
    GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC
    AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC
    GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT
    CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC
    AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT
    GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT
    GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG
    ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC
    CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT
    TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA
    CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA
    CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG
    CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG
    ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA
    GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT
    TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG
    CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC
    CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG
    GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC
    ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG
    AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC
    CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC
    AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC
    ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC
    CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG
    GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC
    TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG
    CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC
    GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC
    CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG
    TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG
    GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG
    CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC
    CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC
    CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG
    ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG
    TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG
    GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC
    GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG
    GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC
    TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG
    CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG
    AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC
    GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC
    GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC
    GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG
    CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC
    GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC
    CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT
    AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG
    TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC
    TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG
    CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG
    CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT
    GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG
    CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG
    AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC
    ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC
    CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC
    ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC
    CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41023
    Clone ID Related Accession (Same CDS sequence) XM_014120825.2 , XM_014120825.1
    Accession Version XM_014120825.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product transcriptional activator GLI3 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726091.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
    GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG
    CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG
    CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA
    AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT
    TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC
    CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG
    GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG
    ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT
    GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC
    AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC
    GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT
    CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC
    AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT
    GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT
    GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG
    ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC
    CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT
    TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA
    CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA
    CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG
    CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG
    ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA
    GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT
    TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG
    CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC
    CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG
    GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC
    ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG
    AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC
    CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC
    AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC
    ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC
    CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG
    GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC
    TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG
    CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC
    GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC
    CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG
    TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG
    GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG
    CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC
    CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC
    CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG
    ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG
    TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG
    GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC
    GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG
    GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC
    TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG
    CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG
    AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC
    GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC
    GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC
    GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG
    CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC
    GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC
    CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT
    AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG
    TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC
    TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG
    CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG
    CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT
    GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG
    CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG
    AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC
    ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC
    CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC
    ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC
    CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976300.1
    CDS271..4869
    Translation

    Target ORF information:

    RefSeq Version XM_014120825.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120825.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
    GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG
    CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG
    CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA
    AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT
    TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC
    CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC
    TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG
    GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG
    ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT
    GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC
    AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC
    GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT
    GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT
    CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC
    ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC
    AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT
    GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT
    GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC
    CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG
    ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC
    CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT
    TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA
    CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA
    CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG
    CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG
    ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA
    GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT
    TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG
    CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC
    CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG
    GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC
    ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG
    AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC
    CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC
    AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC
    ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC
    CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG
    GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC
    TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG
    CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC
    GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC
    CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG
    TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG
    GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG
    CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC
    CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC
    CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG
    ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG
    TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG
    GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC
    GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG
    GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC
    TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG
    CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG
    AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC
    GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC
    GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC
    GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG
    CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC
    GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC
    CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT
    AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG
    TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC
    TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG
    CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG
    CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT
    GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG
    CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG
    AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC
    ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC
    CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC
    ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC
    CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.