TNRC6B gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014117339.1	XP_013972814.1	trinucleotide repeat-containing gene 6B protein isoform X1
XM_022424394.1	XP_022280102.1	trinucleotide repeat-containing gene 6B protein isoform X10
XM_022424397.1	XP_022280105.1	trinucleotide repeat-containing gene 6B protein isoform X12
XM_014117344.1	XP_013972819.1	trinucleotide repeat-containing gene 6B protein isoform X6
XM_014117345.1	XP_013972820.1	trinucleotide repeat-containing gene 6B protein isoform X7
XM_014117342.1	XP_013972817.1	trinucleotide repeat-containing gene 6B protein isoform X4
XM_014117343.1	XP_013972818.1	trinucleotide repeat-containing gene 6B protein isoform X5
XM_014117340.1	XP_013972815.1	trinucleotide repeat-containing gene 6B protein isoform X1
XM_005625852.2	XP_005625909.2	trinucleotide repeat-containing gene 6B protein isoform X2
XM_014117346.1	XP_013972821.1	trinucleotide repeat-containing gene 6B protein isoform X8
XM_022424386.1	XP_022280094.1	trinucleotide repeat-containing gene 6B protein isoform X1
XM_022424387.1	XP_022280095.1	trinucleotide repeat-containing gene 6B protein isoform X2
XM_022424389.1	XP_022280097.1	trinucleotide repeat-containing gene 6B protein isoform X4
XM_014117339.2	XP_013972814.1	trinucleotide repeat-containing gene 6B protein isoform X6
XM_022424392.1	XP_022280100.1	trinucleotide repeat-containing gene 6B protein isoform X8
XM_022424396.1	XP_022280104.1	trinucleotide repeat-containing gene 6B protein isoform X2
XM_014117341.1	XP_013972816.1	trinucleotide repeat-containing gene 6B protein isoform X3
XM_022424388.1	XP_022280096.1	trinucleotide repeat-containing gene 6B protein isoform X3
XM_022424390.1	XP_022280098.1	trinucleotide repeat-containing gene 6B protein isoform X5
XM_022424391.1	XP_022280099.1	trinucleotide repeat-containing gene 6B protein isoform X7
XM_022424393.1	XP_022280101.1	trinucleotide repeat-containing gene 6B protein isoform X9
XM_022424395.1	XP_022280103.1	trinucleotide repeat-containing gene 6B protein isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe13445	XM_014117343.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe291426	XM_022424386.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe291427	XM_022424387.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe291427	XM_022424396.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe291428	XM_022424388.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe291429	XM_022424389.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe291430	XM_022424390.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe291431	XM_022424391.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe291432	XM_022424392.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe291433	XM_022424393.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe291434	XM_022424394.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe291435	XM_022424395.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe38893	XM_014117340.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe38893	XM_014117339.2 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe38894	XM_005625852.2 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe38895	XM_014117341.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe38896	XM_014117342.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe38897	XM_014117344.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe38898	XM_022424397.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe38898	XM_014117345.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe38899	XM_014117346.1 Latest version!	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OCe38893	XM_014117339.1	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

TNRC6B ( XM_014117343.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972818.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe13445
Clone ID Related Accession (Same CDS sequence)	XM_014117343.1
Accession Version	XM_014117343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC 
ACGGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTTAAGCCA ACCAACGGCC 
GCCAGCCCAA TTGGCAGCTC TCCATCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCAAGC GCCGCCCGCT ACATGCCTCG GGAGGTGCCG 
CCGCGATTCC GTTGCCAGCA GGACCACAAA GTGTTACTAA AACGTGGGCA GCCCCCTCCG 
CCGTCCTGCA TGCTCCTTGG GGGCGGAGCA GGGCCTCCTC CCTCCACAGC ACCTGGAGCA 
AACTCAAACA ACGCACAAGT GACAGGAACG CTGCTGCAGA GTGACAGTGG GACTGCACCA 
GAGTCAACCC TTGGAGGTGC TGCTGCTTCA AATTATGCAA ATTCCACTTG GGGCCCGGGA 
GCCTCCTCCA ACAACGGCGC CTCCCCCAAC CCAATTCACA CCTGGGACAA GGTGATTGTA 
GACGGGTCTG ACGTGGAAGA GTGGCCTTGT ATTGCCAGCA AAGACACTGA GTCTTCTTCC 
GAAAACACCA CCGATAACAA CAGTGCCTCG AACCCTGGCT CTGAGAAGAG CACTCTGCCA 
GGAAGCACCA CTAGTAACAA AGGAAAAGGG AGCCAGTGCC AGTCTGCAAG TTCTGGGAAC 
GAATGTAATC TTGGGGTCTG GAAATCTGAC CCTAAGACTA AATCTGTTCA ATCTTCCAAC 
TCTACAACAG AGAGCAACAA TGGACTAGGA AATTGGAGGA ATGTGAGTGG TCAGGATAGA 
ATTGGACCTG GCTCTGGCTT CAGCAACTTT AACCCAAATA GCAACCCATC TGCCTGGCCA 
GCGCTGGTCC AAGAAGGAAA TTCTAGGAAA GGGGCATTGG AAACGGATAC TAGTAATTCC 
AGTGCACAGG TTAGCACAGT AGGTCAGGCA TCCAGGGAAC AGCAGTCAAA GATGGAAAAT 
GCGGGTGTTA ATTTTGTTGT CTCTGGCAGA GAACAGGCTC AGATTCATAA CACTGATGGA 
CCAAAAAATG GAAACACTAA CTCCTTGAAC TTAAGTTCAC CAAACCCCAT GGAGAATAAG 
GGAATGCCCT TTGGAATGGG CTTGGGGAAC GCCTCCAGGA GCACTGATGC CCCTTCACAA 
AGCACTGGAG ATCGAAAGAC TGGGAGTGTT GGATCTTGGG GTGCAGCTAG GGGGCCTTCT 
GGAACTGACA CAGCCTCTGG ACAAAGCAAT TCTGGAAACA ATGGGAACAA TGGAAAAGAT 
AGAGAGGACT CCTGGAAAGG AGCTTCTGTT CAGAAATCAT CTGGGTCAAA AAATGACTCT 
TGGGACAATA ACAGGTCTAC GGGTGGGTCC TGGAACTTTG GCCCTCAGGA CTCTAATGAC 
AACAAATGGG GTGAAGGGAA CAAAATGACA TCTGGGGTCT CTCAGGGAGA ATGGAAACAG 
CCGACTGGGT CTGATGAGTT GAAAATTGGA GAATGGAGTG GTCCAAACCA ACCAAATTCT 
AGCACTGGAG CATGGGACAA TCAAAAGGGC CACCCCCTCC CTGAAAACCA AGGCAATGCC 
CAGGCTCCCT GTTGGGGAAG ATCTTCCAGC TCCACAGGAA GTGAAGTTGG AGGTCAAAGC 
ACTGGAAGCA ACCACAAAGC AGGAAGTAGT GACAGTCATA ATTCTGGCCG TCGGTCATAC 
CGGCCCACAC ATCCTGATTG TCAGGCTGTC TTACAGACTC TTTTGAGCCG AACTGATTTG 
GACCCCAGGG TGCTCTCAAA CACTGGCTGG GGCCAAACTC AAATTAAGCA GGACACTGTG 
TGGGATATTG AAGAGGTGCC AAGGCCTGAG GGGAAATCGG ACAAAGGAAC TGAGGGGTGG 
GAGAGCGCTG CCACACAGAC CAAGAACTCA GGGGGCTGGG GAGATGCACC CAGCCAAAGC 
AATCAAATGA AGTCTGGATG GGGGGAGCTC TCAGCCTCTA CAGAGTGGAA AGACCCCAAG 
AACACAGGAG GCTGGAATGA CTATAAGAAC AACAACTCTT CCAACTGGGG AGGCGGACGA 
CCAGATGAAA AGACATCTTC CTCTTGGAAT GAGAACTCCA GCAAGGATCA GGGGTGGGGA 
GGGGGACGCC AGCCCAATCA AGGATGGACT TCTGGGAAGA ATGGTTGGGG AGAGGAAGTT 
GATCAGGCGA AAAACAGCAG TTGGGAAAGT TCTGCAAGCA AACCTGTGTC TGGATGGGGA 
GAAGGAGGGC AGAATGAAAT TGGAACTTGG GGGAACAGCG GGAATGCAAG CCTAGCTTCC 
AAAGGTGGGT GGGAAGATTG CAAAAGGTCT CCAGCGTGGA CTGAGACTGG CAGACAGCCC 
AATTCCTGGC ATAAACAGCA CCAACAGCAG CAGCAGCCAC AGCAGCCACC GCCACCACAA 
CCAGAGGCTT CTGGTTCATG GGGAGGCCCC CCACCACCAC CTCCAGGCAA CGGACGACCT 
TCCAATTCCA ACTGGAGCAG TGGGCCACAG CCAGTAACCC CTAAGGACGA GGAACCCAGT 
GGTTGGGAAG AGCCATCCCC ACAGTCAATT AGTCGGAAAA TGGACATCGA TGATGGCACT 
TCAGCATGGG GAGACCCTAA CAGCTATAAC TACAAGAATG TGAATCTGTG GGATAAGAAT 
TCCCAAGGGG GCCCAGCACC TCGAGAACCA AACCTGCCTA CCCCGATGAC CAGTAAATCA 
GCATCAGTCT GGAGCAAAAG CACACCACCT GCTCCAGATA ATGGTACTTC AGCTTGGGGT 
GAGCCAAACG AAAGCAGTCC TGGGTGGGGC GAAATGGATG ACACGGGAGC ATCGACCACG 
GGCTGGGGGA ATGCACCCTC CAACGCTCCA AATGCCATGA AACCTAATTC CAAATCTATG 
CAAGACGGCT GGGGGGAGAG TGACGGTCCA GTGACGGGAA CTCGCCATCC CAGCTGGGAG 
GAGGAGGATG ATGGAGGAGT CTGGAACACC GCCGGCTCTC AGGGAAGTGC TTCCTCCCAC 
AACTCAGCAA GCTGGGGACA AGGAGGAAAG AAGCAAATGA AGTGCTCACT GAAAGGAGGA 
AACAATGATT CATGGATGAA TCCTCTTGCC AAACAGTTTT CAAATATGGG ATTGCTGAGT 
CAAACTGAAG ATAATCCAGG CAGCAAAATG GATTTGTCTG TAGGGAGCCT TCCAGATAAA 
AAATTTGACG TGGACAAGCG AGCAATGAAT CTCGGGGATT TTAATGATAT CATGAGGAAG 
GATCGATCTG GGTTCCGTCC ACCTAATTCC AAAGACATGG GAACCACAGA TAGTGGGCCT 
TATTTCGAGA AGCTGACTTT GCCTTTCTCC AATCAAGATG GGTGCCTTGG GGATGAGGCT 
CCCTGCTCTC CCTTCTCCCC TTCTCCCAGC TACAAGCTGT CTCCCTCTGG TTCCACACTA 
CCCAACGTCA GCCTTGGAGC AATCGGCACA GGGCTCAACC CCCAAAACTT CGCTGCTAGA 
CAAGGTGGCA ATCATGGTTT GTTTGGAAAT AGCACAGCAC AATCGAGAGG TATGCACACA 
CCAGTGCAGC CACTAAATTC TTCTCCTAAT CTCCGGGCGC AAGTGCCTCC CCAGTTTATT 
TCCCCCCAGG TTTCTGCCTC CATGCTCAAG CAGTTTCCCA ACAGTGGCCT GAATCCAGGT 
CTTTTCAATG TGGGGCCCCA GTTATCTCCT CAACAGATTG CCATGCTGAG CCAGCTTCCA 
CAAATTCCCC AGTTTCAGTT GGCATGTCAG CTTCTCCTGC AGCAGCAGCA ACAGCAGCAG 
TTGCTACAGA ACCAGAGGAA GCTTTCTCAA GCTGTGCGCC AGCAGCAAGA GCAGCAGCTG 
GCTCGAATGG TGAGCGCTCT CCAGCAACAG CAGCAACAGC AGCAGCAGCA GCAGCAGAGG 
CAGCCTGGCA TGAAGCACTC ACCCTCTCAT CCTGTTGGGC CCAAGCCACA TCTGGACAAC 
ATGGTACCCA ACGCTCTGAA TGTGGGGCTC CCAGACCTTC AAACCAAAGG GCCAATACCT 
GGATATGGTT CTGGCTTCAG CTCTGGCGGC ATGGACTATG GCATGGTTGG TGGGAAGGAG 
GCTGGAACAG AGTCTCGCTT TAAACAGTGG ACCTCCATGA TGGAGGGACT GCCCTCTGTA 
GCCACACAGG AAGCCAATAT GCACAAAAAT GGCGCTATTG TGGCCCCTGG TAAGACTCGA 
GGAGGGTCAC CGTACAACCA GTTTGATATC ATCCCGAGTG ACACACTGGG TGGCCATACG 
GGTCCTGCTG GTGATAGCTG GTTACCTGCC AAATCTCCAC CAACAAATAA AATCGGAAGT 
AAATCCAGCA ATGCCAGTTG GCCTCCAGAA TTCCAACCAG GAGTGCCATG GAAAGGTATC 
CAAAACATTG ACCCTGAATC TGACCCCTAT GTCACCCCAG GAAGTGTACT GGGGGGTACA 
GCCACATCTC CCATTGTAGA TACTGACCAC CAACTTCTGC GGGATAACAC CACAGGGTCT 
AATTCTTCCC TCAACACCTC GCTGCCTTCA CCTGGTGCCT GGCCCTACAG TGCCTCTGAC 
AACTCCTTTA CCAACGTTCA TAGCACTTCA GCAAAGTTCC CTGATTACAA ATCAACATGG 
TCCCCAGATC CCATAGGACA CAACCCCACT CATCTCTCCA ACAAGATGTG GAAAAACCAT 
ATTTCCTCAA GGAACACTAC ACCGCTGCCC CGCCCACCTC CCGGTCTGAC CAACCCCAAA 
CCATCATCTC CCTGGAGCAG CACAGCACCC CGATCAGTCA GGGGGTGGGG GACACAGGAC 
TCACGGCTTG CCTCGGCCTC TACCTGGAGT GATGGTGGTT CAGTTCGTCC CAGTTACTGG 
CTGGTTCTTC ACAATCTCAC TCCACAGATT GATGGGTCAA CGTTGAGAAC CATCTGCATG 
CAGCATGGCC CACTGCTGAC ATTCCATCTG AACCTAACCC AGGGCACTGC CCTGATCCGA 
TACAGCACCA AACAGGAGGC GGCCAAGGCC CAAACTGCAC TGCACATGTG TGTGTTGGGA 
AACACTACCA TCCTGGCTGA GTTTGCCACC GATGATGAAG TTAGCCGCTT TCTGGCACAA 
GCTCAGCCCC CTACACCTGC AGCAACCCCA AGTGCACCCG CCGCAGGTTG GCAGTCGCTG 
GAGACCGGCC AGACCCAGTC AGATCCCGTC GGACCTGCTT TGAATCTTTT TGGTGGGTCC 
ACAGGGCTCG GGCAATGGAG CAGCAGTGCT GGTGGCAGCA GCGGGGCCGA TCTTGCTGGC 
GCTTCGTTGT GGGGGCCCCC AAACTATTCT TCTAGCTTGT GGGGAGTCCC AGCTGTGGAA 
GATCCCCATA GGATGGGCAG CCCTGCTCCC TTACTACCTG GTGACCTTCT GGGAGGAGGG 
TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972818.1
CDS	253..5727
Translation	MEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_014117343.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117343.1

ORF Insert Sequence:

ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC 
ACGGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTTAAGCCA ACCAACGGCC 
GCCAGCCCAA TTGGCAGCTC TCCATCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCAAGC GCCGCCCGCT ACATGCCTCG GGAGGTGCCG 
CCGCGATTCC GTTGCCAGCA GGACCACAAA GTGTTACTAA AACGTGGGCA GCCCCCTCCG 
CCGTCCTGCA TGCTCCTTGG GGGCGGAGCA GGGCCTCCTC CCTCCACAGC ACCTGGAGCA 
AACTCAAACA ACGCACAAGT GACAGGAACG CTGCTGCAGA GTGACAGTGG GACTGCACCA 
GAGTCAACCC TTGGAGGTGC TGCTGCTTCA AATTATGCAA ATTCCACTTG GGGCCCGGGA 
GCCTCCTCCA ACAACGGCGC CTCCCCCAAC CCAATTCACA CCTGGGACAA GGTGATTGTA 
GACGGGTCTG ACGTGGAAGA GTGGCCTTGT ATTGCCAGCA AAGACACTGA GTCTTCTTCC 
GAAAACACCA CCGATAACAA CAGTGCCTCG AACCCTGGCT CTGAGAAGAG CACTCTGCCA 
GGAAGCACCA CTAGTAACAA AGGAAAAGGG AGCCAGTGCC AGTCTGCAAG TTCTGGGAAC 
GAATGTAATC TTGGGGTCTG GAAATCTGAC CCTAAGACTA AATCTGTTCA ATCTTCCAAC 
TCTACAACAG AGAGCAACAA TGGACTAGGA AATTGGAGGA ATGTGAGTGG TCAGGATAGA 
ATTGGACCTG GCTCTGGCTT CAGCAACTTT AACCCAAATA GCAACCCATC TGCCTGGCCA 
GCGCTGGTCC AAGAAGGAAA TTCTAGGAAA GGGGCATTGG AAACGGATAC TAGTAATTCC 
AGTGCACAGG TTAGCACAGT AGGTCAGGCA TCCAGGGAAC AGCAGTCAAA GATGGAAAAT 
GCGGGTGTTA ATTTTGTTGT CTCTGGCAGA GAACAGGCTC AGATTCATAA CACTGATGGA 
CCAAAAAATG GAAACACTAA CTCCTTGAAC TTAAGTTCAC CAAACCCCAT GGAGAATAAG 
GGAATGCCCT TTGGAATGGG CTTGGGGAAC GCCTCCAGGA GCACTGATGC CCCTTCACAA 
AGCACTGGAG ATCGAAAGAC TGGGAGTGTT GGATCTTGGG GTGCAGCTAG GGGGCCTTCT 
GGAACTGACA CAGCCTCTGG ACAAAGCAAT TCTGGAAACA ATGGGAACAA TGGAAAAGAT 
AGAGAGGACT CCTGGAAAGG AGCTTCTGTT CAGAAATCAT CTGGGTCAAA AAATGACTCT 
TGGGACAATA ACAGGTCTAC GGGTGGGTCC TGGAACTTTG GCCCTCAGGA CTCTAATGAC 
AACAAATGGG GTGAAGGGAA CAAAATGACA TCTGGGGTCT CTCAGGGAGA ATGGAAACAG 
CCGACTGGGT CTGATGAGTT GAAAATTGGA GAATGGAGTG GTCCAAACCA ACCAAATTCT 
AGCACTGGAG CATGGGACAA TCAAAAGGGC CACCCCCTCC CTGAAAACCA AGGCAATGCC 
CAGGCTCCCT GTTGGGGAAG ATCTTCCAGC TCCACAGGAA GTGAAGTTGG AGGTCAAAGC 
ACTGGAAGCA ACCACAAAGC AGGAAGTAGT GACAGTCATA ATTCTGGCCG TCGGTCATAC 
CGGCCCACAC ATCCTGATTG TCAGGCTGTC TTACAGACTC TTTTGAGCCG AACTGATTTG 
GACCCCAGGG TGCTCTCAAA CACTGGCTGG GGCCAAACTC AAATTAAGCA GGACACTGTG 
TGGGATATTG AAGAGGTGCC AAGGCCTGAG GGGAAATCGG ACAAAGGAAC TGAGGGGTGG 
GAGAGCGCTG CCACACAGAC CAAGAACTCA GGGGGCTGGG GAGATGCACC CAGCCAAAGC 
AATCAAATGA AGTCTGGATG GGGGGAGCTC TCAGCCTCTA CAGAGTGGAA AGACCCCAAG 
AACACAGGAG GCTGGAATGA CTATAAGAAC AACAACTCTT CCAACTGGGG AGGCGGACGA 
CCAGATGAAA AGACATCTTC CTCTTGGAAT GAGAACTCCA GCAAGGATCA GGGGTGGGGA 
GGGGGACGCC AGCCCAATCA AGGATGGACT TCTGGGAAGA ATGGTTGGGG AGAGGAAGTT 
GATCAGGCGA AAAACAGCAG TTGGGAAAGT TCTGCAAGCA AACCTGTGTC TGGATGGGGA 
GAAGGAGGGC AGAATGAAAT TGGAACTTGG GGGAACAGCG GGAATGCAAG CCTAGCTTCC 
AAAGGTGGGT GGGAAGATTG CAAAAGGTCT CCAGCGTGGA CTGAGACTGG CAGACAGCCC 
AATTCCTGGC ATAAACAGCA CCAACAGCAG CAGCAGCCAC AGCAGCCACC GCCACCACAA 
CCAGAGGCTT CTGGTTCATG GGGAGGCCCC CCACCACCAC CTCCAGGCAA CGGACGACCT 
TCCAATTCCA ACTGGAGCAG TGGGCCACAG CCAGTAACCC CTAAGGACGA GGAACCCAGT 
GGTTGGGAAG AGCCATCCCC ACAGTCAATT AGTCGGAAAA TGGACATCGA TGATGGCACT 
TCAGCATGGG GAGACCCTAA CAGCTATAAC TACAAGAATG TGAATCTGTG GGATAAGAAT 
TCCCAAGGGG GCCCAGCACC TCGAGAACCA AACCTGCCTA CCCCGATGAC CAGTAAATCA 
GCATCAGTCT GGAGCAAAAG CACACCACCT GCTCCAGATA ATGGTACTTC AGCTTGGGGT 
GAGCCAAACG AAAGCAGTCC TGGGTGGGGC GAAATGGATG ACACGGGAGC ATCGACCACG 
GGCTGGGGGA ATGCACCCTC CAACGCTCCA AATGCCATGA AACCTAATTC CAAATCTATG 
CAAGACGGCT GGGGGGAGAG TGACGGTCCA GTGACGGGAA CTCGCCATCC CAGCTGGGAG 
GAGGAGGATG ATGGAGGAGT CTGGAACACC GCCGGCTCTC AGGGAAGTGC TTCCTCCCAC 
AACTCAGCAA GCTGGGGACA AGGAGGAAAG AAGCAAATGA AGTGCTCACT GAAAGGAGGA 
AACAATGATT CATGGATGAA TCCTCTTGCC AAACAGTTTT CAAATATGGG ATTGCTGAGT 
CAAACTGAAG ATAATCCAGG CAGCAAAATG GATTTGTCTG TAGGGAGCCT TCCAGATAAA 
AAATTTGACG TGGACAAGCG AGCAATGAAT CTCGGGGATT TTAATGATAT CATGAGGAAG 
GATCGATCTG GGTTCCGTCC ACCTAATTCC AAAGACATGG GAACCACAGA TAGTGGGCCT 
TATTTCGAGA AGCTGACTTT GCCTTTCTCC AATCAAGATG GGTGCCTTGG GGATGAGGCT 
CCCTGCTCTC CCTTCTCCCC TTCTCCCAGC TACAAGCTGT CTCCCTCTGG TTCCACACTA 
CCCAACGTCA GCCTTGGAGC AATCGGCACA GGGCTCAACC CCCAAAACTT CGCTGCTAGA 
CAAGGTGGCA ATCATGGTTT GTTTGGAAAT AGCACAGCAC AATCGAGAGG TATGCACACA 
CCAGTGCAGC CACTAAATTC TTCTCCTAAT CTCCGGGCGC AAGTGCCTCC CCAGTTTATT 
TCCCCCCAGG TTTCTGCCTC CATGCTCAAG CAGTTTCCCA ACAGTGGCCT GAATCCAGGT 
CTTTTCAATG TGGGGCCCCA GTTATCTCCT CAACAGATTG CCATGCTGAG CCAGCTTCCA 
CAAATTCCCC AGTTTCAGTT GGCATGTCAG CTTCTCCTGC AGCAGCAGCA ACAGCAGCAG 
TTGCTACAGA ACCAGAGGAA GCTTTCTCAA GCTGTGCGCC AGCAGCAAGA GCAGCAGCTG 
GCTCGAATGG TGAGCGCTCT CCAGCAACAG CAGCAACAGC AGCAGCAGCA GCAGCAGAGG 
CAGCCTGGCA TGAAGCACTC ACCCTCTCAT CCTGTTGGGC CCAAGCCACA TCTGGACAAC 
ATGGTACCCA ACGCTCTGAA TGTGGGGCTC CCAGACCTTC AAACCAAAGG GCCAATACCT 
GGATATGGTT CTGGCTTCAG CTCTGGCGGC ATGGACTATG GCATGGTTGG TGGGAAGGAG 
GCTGGAACAG AGTCTCGCTT TAAACAGTGG ACCTCCATGA TGGAGGGACT GCCCTCTGTA 
GCCACACAGG AAGCCAATAT GCACAAAAAT GGCGCTATTG TGGCCCCTGG TAAGACTCGA 
GGAGGGTCAC CGTACAACCA GTTTGATATC ATCCCGAGTG ACACACTGGG TGGCCATACG 
GGTCCTGCTG GTGATAGCTG GTTACCTGCC AAATCTCCAC CAACAAATAA AATCGGAAGT 
AAATCCAGCA ATGCCAGTTG GCCTCCAGAA TTCCAACCAG GAGTGCCATG GAAAGGTATC 
CAAAACATTG ACCCTGAATC TGACCCCTAT GTCACCCCAG GAAGTGTACT GGGGGGTACA 
GCCACATCTC CCATTGTAGA TACTGACCAC CAACTTCTGC GGGATAACAC CACAGGGTCT 
AATTCTTCCC TCAACACCTC GCTGCCTTCA CCTGGTGCCT GGCCCTACAG TGCCTCTGAC 
AACTCCTTTA CCAACGTTCA TAGCACTTCA GCAAAGTTCC CTGATTACAA ATCAACATGG 
TCCCCAGATC CCATAGGACA CAACCCCACT CATCTCTCCA ACAAGATGTG GAAAAACCAT 
ATTTCCTCAA GGAACACTAC ACCGCTGCCC CGCCCACCTC CCGGTCTGAC CAACCCCAAA 
CCATCATCTC CCTGGAGCAG CACAGCACCC CGATCAGTCA GGGGGTGGGG GACACAGGAC 
TCACGGCTTG CCTCGGCCTC TACCTGGAGT GATGGTGGTT CAGTTCGTCC CAGTTACTGG 
CTGGTTCTTC ACAATCTCAC TCCACAGATT GATGGGTCAA CGTTGAGAAC CATCTGCATG 
CAGCATGGCC CACTGCTGAC ATTCCATCTG AACCTAACCC AGGGCACTGC CCTGATCCGA 
TACAGCACCA AACAGGAGGC GGCCAAGGCC CAAACTGCAC TGCACATGTG TGTGTTGGGA 
AACACTACCA TCCTGGCTGA GTTTGCCACC GATGATGAAG TTAGCCGCTT TCTGGCACAA 
GCTCAGCCCC CTACACCTGC AGCAACCCCA AGTGCACCCG CCGCAGGTTG GCAGTCGCTG 
GAGACCGGCC AGACCCAGTC AGATCCCGTC GGACCTGCTT TGAATCTTTT TGGTGGGTCC 
ACAGGGCTCG GGCAATGGAG CAGCAGTGCT GGTGGCAGCA GCGGGGCCGA TCTTGCTGGC 
GCTTCGTTGT GGGGGCCCCC AAACTATTCT TCTAGCTTGT GGGGAGTCCC AGCTGTGGAA 
GATCCCCATA GGATGGGCAG CCCTGCTCCC TTACTACCTG GTGACCTTCT GGGAGGAGGG 
TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424386.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280094.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

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CloneID	OCe291426
Clone ID Related Accession (Same CDS sequence)	XM_022424386.1
Accession Version	XM_022424386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC ATCAGGATTG CTTCTGGCTG TATGACCCAC CTTCCTCTTT CCATCAATCC 
ATGTCCCACC ATTTTCCATT CCATAACAGA AGTCAGCTTC ATGAGAATAC TCTTTGCTTC 
TACTTTAATC TCTTCCAGGG TGCAGTGATC CAGAATCCAG AAGGGCTGTT AGTTGAGAGA 
GAGAAGGAGC AAGAAAGGGA AGAACAGTTA ATGGAAGACA AGAAAAGGAA GAAAGAGGAT 
AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC ACGGAACAAA AAACCAAAGC ACCTGACATT 
CTGAAGCATG CTGTGAACCA CACACTGATT CTGGGATGTC TTAAGTTTGG ACTTCAAGAA 
GCAGTGCCCG AAGTGACGAA ACCAAGTTTA AGCCAACCAA CGGCCGCCAG CCCAATTGGC 
AGCTCTCCAT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CAAGCGCCGC CCGCTACATG CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC 
CAGCAGGACC ACAAAGTGTT ACTAAAACGT GGGCAGCCCC CTCCGCCGTC CTGCATGCTC 
CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC ACAGCACCTG GAGCAAACTC AAACAACGCA 
CAAGTGACAG GAACGCTGCT GCAGAGTGAC AGTGGGACTG CACCAGAGTC AACCCTTGGA 
GGTGCTGCTG CTTCAAATTA TGCAAATTCC ACTTGGGGCC CGGGAGCCTC CTCCAACAAC 
GGCGCCTCCC CCAACCCAAT TCACACCTGG GACAAGGTGA TTGTAGACGG GTCTGACGTG 
GAAGAGTGGC CTTGTATTGC CAGCAAAGAC ACTGAGTCTT CTTCCGAAAA CACCACCGAT 
AACAACAGTG CCTCGAACCC TGGCTCTGAG AAGAGCACTC TGCCAGGAAG CACCACTAGT 
AACAAAGGAA AAGGGAGCCA GTGCCAGTCT GCAAGTTCTG GGAACGAATG TAATCTTGGG 
GTCTGGAAAT CTGACCCTAA GACTAAATCT GTTCAATCTT CCAACTCTAC AACAGAGAGC 
AACAATGGAC TAGGAAATTG GAGGAATGTG AGTGGTCAGG ATAGAATTGG ACCTGGCTCT 
GGCTTCAGCA ACTTTAACCC AAATAGCAAC CCATCTGCCT GGCCAGCGCT GGTCCAAGAA 
GGAAATTCTA GGAAAGGGGC ATTGGAAACG GATACTAGTA ATTCCAGTGC ACAGGTTAGC 
ACAGTAGGTC AGGCATCCAG GGAACAGCAG TCAAAGATGG AAAATGCGGG TGTTAATTTT 
GTTGTCTCTG GCAGAGAACA GGCTCAGATT CATAACACTG ATGGACCAAA AAATGGAAAC 
ACTAACTCCT TGAACTTAAG TTCACCAAAC CCCATGGAGA ATAAGGGAAT GCCCTTTGGA 
ATGGGCTTGG GGAACGCCTC CAGGAGCACT GATGCCCCTT CACAAAGCAC TGGAGATCGA 
AAGACTGGGA GTGTTGGATC TTGGGGTGCA GCTAGGGGGC CTTCTGGAAC TGACACAGCC 
TCTGGACAAA GCAATTCTGG AAACAATGGG AACAATGGAA AAGATAGAGA GGACTCCTGG 
AAAGGAGCTT CTGTTCAGAA ATCATCTGGG TCAAAAAATG ACTCTTGGGA CAATAACAGG 
TCTACGGGTG GGTCCTGGAA CTTTGGCCCT CAGGACTCTA ATGACAACAA ATGGGGTGAA 
GGGAACAAAA TGACATCTGG GGTCTCTCAG GGAGAATGGA AACAGCCGAC TGGGTCTGAT 
GAGTTGAAAA TTGGAGAATG GAGTGGTCCA AACCAACCAA ATTCTAGCAC TGGAGCATGG 
GACAATCAAA AGGGCCACCC CCTCCCTGAA AACCAAGGCA ATGCCCAGGC TCCCTGTTGG 
GGAAGATCTT CCAGCTCCAC AGGAAGTGAA GTTGGAGGTC AAAGCACTGG AAGCAACCAC 
AAAGCAGGAA GTAGTGACAG TCATAATTCT GGCCGTCGGT CATACCGGCC CACACATCCT 
GATTGTCAGG CTGTCTTACA GACTCTTTTG AGCCGAACTG ATTTGGACCC CAGGGTGCTC 
TCAAACACTG GCTGGGGCCA AACTCAAATT AAGCAGGACA CTGTGTGGGA TATTGAAGAG 
GTGCCAAGGC CTGAGGGGAA ATCGGACAAA GGAACTGAGG GGTGGGAGAG CGCTGCCACA 
CAGACCAAGA ACTCAGGGGG CTGGGGAGAT GCACCCAGCC AAAGCAATCA AATGAAGTCT 
GGATGGGGGG AGCTCTCAGC CTCTACAGAG TGGAAAGACC CCAAGAACAC AGGAGGCTGG 
AATGACTATA AGAACAACAA CTCTTCCAAC TGGGGAGGCG GACGACCAGA TGAAAAGACA 
TCTTCCTCTT GGAATGAGAA CTCCAGCAAG GATCAGGGGT GGGGAGGGGG ACGCCAGCCC 
AATCAAGGAT GGACTTCTGG GAAGAATGGT TGGGGAGAGG AAGTTGATCA GGCGAAAAAC 
AGCAGTTGGG AAAGTTCTGC AAGCAAACCT GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT 
GAAATTGGAA CTTGGGGGAA CAGCGGGAAT GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA 
GATTGCAAAA GGTCTCCAGC GTGGACTGAG ACTGGCAGAC AGCCCAATTC CTGGCATAAA 
CAGCACCAAC AGCAGCAGCA GCCACAGCAG CCACCGCCAC CACAACCAGA GGCTTCTGGT 
TCATGGGGAG GCCCCCCACC ACCACCTCCA GGCAACGGAC GACCTTCCAA TTCCAACTGG 
AGCAGTGGGC CACAGCCAGT AACCCCTAAG GACGAGGAAC CCAGTGGTTG GGAAGAGCCA 
TCCCCACAGT CAATTAGTCG GAAAATGGAC ATCGATGATG GCACTTCAGC ATGGGGAGAC 
CCTAACAGCT ATAACTACAA GAATGTGAAT CTGTGGGATA AGAATTCCCA AGGGGGCCCA 
GCACCTCGAG AACCAAACCT GCCTACCCCG ATGACCAGTA AATCAGCATC AGTCTGGAGC 
AAAAGCACAC CACCTGCTCC AGATAATGGT ACTTCAGCTT GGGGTGAGCC AAACGAAAGC 
AGTCCTGGGT GGGGCGAAAT GGATGACACG GGAGCATCGA CCACGGGCTG GGGGAATGCA 
CCCTCCAACG CTCCAAATGC CATGAAACCT AATTCCAAAT CTATGCAAGA CGGCTGGGGG 
GAGAGTGACG GTCCAGTGAC GGGAACTCGC CATCCCAGCT GGGAGGAGGA GGATGATGGA 
GGAGTCTGGA ACACCGCCGG CTCTCAGGGA AGTGCTTCCT CCCACAACTC AGCAAGCTGG 
GGACAAGGAG GAAAGAAGCA AATGAAGTGC TCACTGAAAG GAGGAAACAA TGATTCATGG 
ATGAATCCTC TTGCCAAACA GTTTTCAAAT ATGGGATTGC TGAGTCAAAC TGAAGATAAT 
CCAGGCAGCA AAATGGATTT GTCTGTAGGG AGCCTTCCAG ATAAAAAATT TGACGTGGAC 
AAGCGAGCAA TGAATCTCGG GGATTTTAAT GATATCATGA GGAAGGATCG ATCTGGGTTC 
CGTCCACCTA ATTCCAAAGA CATGGGAACC ACAGATAGTG GGCCTTATTT CGAGAAGCTG 
ACTTTGCCTT TCTCCAATCA AGATGGGTGC CTTGGGGATG AGGCTCCCTG CTCTCCCTTC 
TCCCCTTCTC CCAGCTACAA GCTGTCTCCC TCTGGTTCCA CACTACCCAA CGTCAGCCTT 
GGAGCAATCG GCACAGGGCT CAACCCCCAA AACTTCGCTG CTAGACAAGG TGGCAATCAT 
GGTTTGTTTG GAAATAGCAC AGCACAATCG AGAGGTATGC ACACACCAGT GCAGCCACTA 
AATTCTTCTC CTAATCTCCG GGCGCAAGTG CCTCCCCAGT TTATTTCCCC CCAGGTTTCT 
GCCTCCATGC TCAAGCAGTT TCCCAACAGT GGCCTGAATC CAGGTCTTTT CAATGTGGGG 
CCCCAGTTAT CTCCTCAACA GATTGCCATG CTGAGCCAGC TTCCACAAAT TCCCCAGTTT 
CAGTTGGCAT GTCAGCTTCT CCTGCAGCAG CAGCAACAGC AGCAGTTGCT ACAGAACCAG 
AGGAAGCTTT CTCAAGCTGT GCGCCAGCAG CAAGAGCAGC AGCTGGCTCG AATGGTGAGC 
GCTCTCCAGC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AGAGGCAGCC TGGCATGAAG 
CACTCACCCT CTCATCCTGT TGGGCCCAAG CCACATCTGG ACAACATGGT ACCCAACGCT 
CTGAATGTGG GGCTCCCAGA CCTTCAAACC AAAGGGCCAA TACCTGGATA TGGTTCTGGC 
TTCAGCTCTG GCGGCATGGA CTATGGCATG GTTGGTGGGA AGGAGGCTGG AACAGAGTCT 
CGCTTTAAAC AGTGGACCTC CATGATGGAG GGACTGCCCT CTGTAGCCAC ACAGGAAGCC 
AATATGCACA AAAATGGCGC TATTGTGGCC CCTGGTAAGA CTCGAGGAGG GTCACCGTAC 
AACCAGTTTG ATATCATCCC GAGTGACACA CTGGGTGGCC ATACGGGTCC TGCTGGTGAT 
AGCTGGTTAC CTGCCAAATC TCCACCAACA AATAAAATCG GAAGTAAATC CAGCAATGCC 
AGTTGGCCTC CAGAATTCCA ACCAGGAGTG CCATGGAAAG GTATCCAAAA CATTGACCCT 
GAATCTGACC CCTATGTCAC CCCAGGAAGT GTACTGGGGG GTACAGCCAC ATCTCCCATT 
GTAGATACTG ACCACCAACT TCTGCGGGAT AACACCACAG GGTCTAATTC TTCCCTCAAC 
ACCTCGCTGC CTTCACCTGG TGCCTGGCCC TACAGTGCCT CTGACAACTC CTTTACCAAC 
GTTCATAGCA CTTCAGCAAA GTTCCCTGAT TACAAATCAA CATGGTCCCC AGATCCCATA 
GGACACAACC CCACTCATCT CTCCAACAAG ATGTGGAAAA ACCATATTTC CTCAAGGAAC 
ACTACACCGC TGCCCCGCCC ACCTCCCGGT CTGACCAACC CCAAACCATC ATCTCCCTGG 
AGCAGCACAG CACCCCGATC AGTCAGGGGG TGGGGGACAC AGGACTCACG GCTTGCCTCG 
GCCTCTACCT GGAGTGATGG TGGTTCAGTT CGTCCCAGTT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATTGATGG GTCAACGTTG AGAACCATCT GCATGCAGCA TGGCCCACTG 
CTGACATTCC ATCTGAACCT AACCCAGGGC ACTGCCCTGA TCCGATACAG CACCAAACAG 
GAGGCGGCCA AGGCCCAAAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTG 
GCTGAGTTTG CCACCGATGA TGAAGTTAGC CGCTTTCTGG CACAAGCTCA GCCCCCTACA 
CCTGCAGCAA CCCCAAGTGC ACCCGCCGCA GGTTGGCAGT CGCTGGAGAC CGGCCAGACC 
CAGTCAGATC CCGTCGGACC TGCTTTGAAT CTTTTTGGTG GGTCCACAGG GCTCGGGCAA 
TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG GCCGATCTTG CTGGCGCTTC GTTGTGGGGG 
CCCCCAAACT ATTCTTCTAG CTTGTGGGGA GTCCCAGCTG TGGAAGATCC CCATAGGATG 
GGCAGCCCTG CTCCCTTACT ACCTGGTGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280094.1
CDS	16..5775
Translation	MDSHQDCFWLYDPPSSFHQSMSHHFPFHNRSQLHENTLCFYFNLFQGAVIQNPEGLLVEREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKAPDILKHAVNHTLILGCLKFGLQEAVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424386.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424386.1

ORF Insert Sequence:

ATGGACTCCC ATCAGGATTG CTTCTGGCTG TATGACCCAC CTTCCTCTTT CCATCAATCC 
ATGTCCCACC ATTTTCCATT CCATAACAGA AGTCAGCTTC ATGAGAATAC TCTTTGCTTC 
TACTTTAATC TCTTCCAGGG TGCAGTGATC CAGAATCCAG AAGGGCTGTT AGTTGAGAGA 
GAGAAGGAGC AAGAAAGGGA AGAACAGTTA ATGGAAGACA AGAAAAGGAA GAAAGAGGAT 
AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC ACGGAACAAA AAACCAAAGC ACCTGACATT 
CTGAAGCATG CTGTGAACCA CACACTGATT CTGGGATGTC TTAAGTTTGG ACTTCAAGAA 
GCAGTGCCCG AAGTGACGAA ACCAAGTTTA AGCCAACCAA CGGCCGCCAG CCCAATTGGC 
AGCTCTCCAT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CAAGCGCCGC CCGCTACATG CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC 
CAGCAGGACC ACAAAGTGTT ACTAAAACGT GGGCAGCCCC CTCCGCCGTC CTGCATGCTC 
CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC ACAGCACCTG GAGCAAACTC AAACAACGCA 
CAAGTGACAG GAACGCTGCT GCAGAGTGAC AGTGGGACTG CACCAGAGTC AACCCTTGGA 
GGTGCTGCTG CTTCAAATTA TGCAAATTCC ACTTGGGGCC CGGGAGCCTC CTCCAACAAC 
GGCGCCTCCC CCAACCCAAT TCACACCTGG GACAAGGTGA TTGTAGACGG GTCTGACGTG 
GAAGAGTGGC CTTGTATTGC CAGCAAAGAC ACTGAGTCTT CTTCCGAAAA CACCACCGAT 
AACAACAGTG CCTCGAACCC TGGCTCTGAG AAGAGCACTC TGCCAGGAAG CACCACTAGT 
AACAAAGGAA AAGGGAGCCA GTGCCAGTCT GCAAGTTCTG GGAACGAATG TAATCTTGGG 
GTCTGGAAAT CTGACCCTAA GACTAAATCT GTTCAATCTT CCAACTCTAC AACAGAGAGC 
AACAATGGAC TAGGAAATTG GAGGAATGTG AGTGGTCAGG ATAGAATTGG ACCTGGCTCT 
GGCTTCAGCA ACTTTAACCC AAATAGCAAC CCATCTGCCT GGCCAGCGCT GGTCCAAGAA 
GGAAATTCTA GGAAAGGGGC ATTGGAAACG GATACTAGTA ATTCCAGTGC ACAGGTTAGC 
ACAGTAGGTC AGGCATCCAG GGAACAGCAG TCAAAGATGG AAAATGCGGG TGTTAATTTT 
GTTGTCTCTG GCAGAGAACA GGCTCAGATT CATAACACTG ATGGACCAAA AAATGGAAAC 
ACTAACTCCT TGAACTTAAG TTCACCAAAC CCCATGGAGA ATAAGGGAAT GCCCTTTGGA 
ATGGGCTTGG GGAACGCCTC CAGGAGCACT GATGCCCCTT CACAAAGCAC TGGAGATCGA 
AAGACTGGGA GTGTTGGATC TTGGGGTGCA GCTAGGGGGC CTTCTGGAAC TGACACAGCC 
TCTGGACAAA GCAATTCTGG AAACAATGGG AACAATGGAA AAGATAGAGA GGACTCCTGG 
AAAGGAGCTT CTGTTCAGAA ATCATCTGGG TCAAAAAATG ACTCTTGGGA CAATAACAGG 
TCTACGGGTG GGTCCTGGAA CTTTGGCCCT CAGGACTCTA ATGACAACAA ATGGGGTGAA 
GGGAACAAAA TGACATCTGG GGTCTCTCAG GGAGAATGGA AACAGCCGAC TGGGTCTGAT 
GAGTTGAAAA TTGGAGAATG GAGTGGTCCA AACCAACCAA ATTCTAGCAC TGGAGCATGG 
GACAATCAAA AGGGCCACCC CCTCCCTGAA AACCAAGGCA ATGCCCAGGC TCCCTGTTGG 
GGAAGATCTT CCAGCTCCAC AGGAAGTGAA GTTGGAGGTC AAAGCACTGG AAGCAACCAC 
AAAGCAGGAA GTAGTGACAG TCATAATTCT GGCCGTCGGT CATACCGGCC CACACATCCT 
GATTGTCAGG CTGTCTTACA GACTCTTTTG AGCCGAACTG ATTTGGACCC CAGGGTGCTC 
TCAAACACTG GCTGGGGCCA AACTCAAATT AAGCAGGACA CTGTGTGGGA TATTGAAGAG 
GTGCCAAGGC CTGAGGGGAA ATCGGACAAA GGAACTGAGG GGTGGGAGAG CGCTGCCACA 
CAGACCAAGA ACTCAGGGGG CTGGGGAGAT GCACCCAGCC AAAGCAATCA AATGAAGTCT 
GGATGGGGGG AGCTCTCAGC CTCTACAGAG TGGAAAGACC CCAAGAACAC AGGAGGCTGG 
AATGACTATA AGAACAACAA CTCTTCCAAC TGGGGAGGCG GACGACCAGA TGAAAAGACA 
TCTTCCTCTT GGAATGAGAA CTCCAGCAAG GATCAGGGGT GGGGAGGGGG ACGCCAGCCC 
AATCAAGGAT GGACTTCTGG GAAGAATGGT TGGGGAGAGG AAGTTGATCA GGCGAAAAAC 
AGCAGTTGGG AAAGTTCTGC AAGCAAACCT GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT 
GAAATTGGAA CTTGGGGGAA CAGCGGGAAT GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA 
GATTGCAAAA GGTCTCCAGC GTGGACTGAG ACTGGCAGAC AGCCCAATTC CTGGCATAAA 
CAGCACCAAC AGCAGCAGCA GCCACAGCAG CCACCGCCAC CACAACCAGA GGCTTCTGGT 
TCATGGGGAG GCCCCCCACC ACCACCTCCA GGCAACGGAC GACCTTCCAA TTCCAACTGG 
AGCAGTGGGC CACAGCCAGT AACCCCTAAG GACGAGGAAC CCAGTGGTTG GGAAGAGCCA 
TCCCCACAGT CAATTAGTCG GAAAATGGAC ATCGATGATG GCACTTCAGC ATGGGGAGAC 
CCTAACAGCT ATAACTACAA GAATGTGAAT CTGTGGGATA AGAATTCCCA AGGGGGCCCA 
GCACCTCGAG AACCAAACCT GCCTACCCCG ATGACCAGTA AATCAGCATC AGTCTGGAGC 
AAAAGCACAC CACCTGCTCC AGATAATGGT ACTTCAGCTT GGGGTGAGCC AAACGAAAGC 
AGTCCTGGGT GGGGCGAAAT GGATGACACG GGAGCATCGA CCACGGGCTG GGGGAATGCA 
CCCTCCAACG CTCCAAATGC CATGAAACCT AATTCCAAAT CTATGCAAGA CGGCTGGGGG 
GAGAGTGACG GTCCAGTGAC GGGAACTCGC CATCCCAGCT GGGAGGAGGA GGATGATGGA 
GGAGTCTGGA ACACCGCCGG CTCTCAGGGA AGTGCTTCCT CCCACAACTC AGCAAGCTGG 
GGACAAGGAG GAAAGAAGCA AATGAAGTGC TCACTGAAAG GAGGAAACAA TGATTCATGG 
ATGAATCCTC TTGCCAAACA GTTTTCAAAT ATGGGATTGC TGAGTCAAAC TGAAGATAAT 
CCAGGCAGCA AAATGGATTT GTCTGTAGGG AGCCTTCCAG ATAAAAAATT TGACGTGGAC 
AAGCGAGCAA TGAATCTCGG GGATTTTAAT GATATCATGA GGAAGGATCG ATCTGGGTTC 
CGTCCACCTA ATTCCAAAGA CATGGGAACC ACAGATAGTG GGCCTTATTT CGAGAAGCTG 
ACTTTGCCTT TCTCCAATCA AGATGGGTGC CTTGGGGATG AGGCTCCCTG CTCTCCCTTC 
TCCCCTTCTC CCAGCTACAA GCTGTCTCCC TCTGGTTCCA CACTACCCAA CGTCAGCCTT 
GGAGCAATCG GCACAGGGCT CAACCCCCAA AACTTCGCTG CTAGACAAGG TGGCAATCAT 
GGTTTGTTTG GAAATAGCAC AGCACAATCG AGAGGTATGC ACACACCAGT GCAGCCACTA 
AATTCTTCTC CTAATCTCCG GGCGCAAGTG CCTCCCCAGT TTATTTCCCC CCAGGTTTCT 
GCCTCCATGC TCAAGCAGTT TCCCAACAGT GGCCTGAATC CAGGTCTTTT CAATGTGGGG 
CCCCAGTTAT CTCCTCAACA GATTGCCATG CTGAGCCAGC TTCCACAAAT TCCCCAGTTT 
CAGTTGGCAT GTCAGCTTCT CCTGCAGCAG CAGCAACAGC AGCAGTTGCT ACAGAACCAG 
AGGAAGCTTT CTCAAGCTGT GCGCCAGCAG CAAGAGCAGC AGCTGGCTCG AATGGTGAGC 
GCTCTCCAGC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AGAGGCAGCC TGGCATGAAG 
CACTCACCCT CTCATCCTGT TGGGCCCAAG CCACATCTGG ACAACATGGT ACCCAACGCT 
CTGAATGTGG GGCTCCCAGA CCTTCAAACC AAAGGGCCAA TACCTGGATA TGGTTCTGGC 
TTCAGCTCTG GCGGCATGGA CTATGGCATG GTTGGTGGGA AGGAGGCTGG AACAGAGTCT 
CGCTTTAAAC AGTGGACCTC CATGATGGAG GGACTGCCCT CTGTAGCCAC ACAGGAAGCC 
AATATGCACA AAAATGGCGC TATTGTGGCC CCTGGTAAGA CTCGAGGAGG GTCACCGTAC 
AACCAGTTTG ATATCATCCC GAGTGACACA CTGGGTGGCC ATACGGGTCC TGCTGGTGAT 
AGCTGGTTAC CTGCCAAATC TCCACCAACA AATAAAATCG GAAGTAAATC CAGCAATGCC 
AGTTGGCCTC CAGAATTCCA ACCAGGAGTG CCATGGAAAG GTATCCAAAA CATTGACCCT 
GAATCTGACC CCTATGTCAC CCCAGGAAGT GTACTGGGGG GTACAGCCAC ATCTCCCATT 
GTAGATACTG ACCACCAACT TCTGCGGGAT AACACCACAG GGTCTAATTC TTCCCTCAAC 
ACCTCGCTGC CTTCACCTGG TGCCTGGCCC TACAGTGCCT CTGACAACTC CTTTACCAAC 
GTTCATAGCA CTTCAGCAAA GTTCCCTGAT TACAAATCAA CATGGTCCCC AGATCCCATA 
GGACACAACC CCACTCATCT CTCCAACAAG ATGTGGAAAA ACCATATTTC CTCAAGGAAC 
ACTACACCGC TGCCCCGCCC ACCTCCCGGT CTGACCAACC CCAAACCATC ATCTCCCTGG 
AGCAGCACAG CACCCCGATC AGTCAGGGGG TGGGGGACAC AGGACTCACG GCTTGCCTCG 
GCCTCTACCT GGAGTGATGG TGGTTCAGTT CGTCCCAGTT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATTGATGG GTCAACGTTG AGAACCATCT GCATGCAGCA TGGCCCACTG 
CTGACATTCC ATCTGAACCT AACCCAGGGC ACTGCCCTGA TCCGATACAG CACCAAACAG 
GAGGCGGCCA AGGCCCAAAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTG 
GCTGAGTTTG CCACCGATGA TGAAGTTAGC CGCTTTCTGG CACAAGCTCA GCCCCCTACA 
CCTGCAGCAA CCCCAAGTGC ACCCGCCGCA GGTTGGCAGT CGCTGGAGAC CGGCCAGACC 
CAGTCAGATC CCGTCGGACC TGCTTTGAAT CTTTTTGGTG GGTCCACAGG GCTCGGGCAA 
TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG GCCGATCTTG CTGGCGCTTC GTTGTGGGGG 
CCCCCAAACT ATTCTTCTAG CTTGTGGGGA GTCCCAGCTG TGGAAGATCC CCATAGGATG 
GGCAGCCCTG CTCCCTTACT ACCTGGTGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424387.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280095.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

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CloneID	OCe291427
Clone ID Related Accession (Same CDS sequence)	XM_022424387.1 , XM_022424396.1
Accession Version	XM_022424387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGCACCTGA CATTCTGAAG 
CATGCTGTGA ACCACACACT GATTCTGGGA TGTCTTAAGT TTGGACTTCA AGAAGCAGTG 
CCCGAAGTGA CGAAACCAAG TTTAAGCCAA CCAACGGCCG CCAGCCCAAT TGGCAGCTCT 
CCATCGCCAC CAGTCAATGG TGGCAACAAT GCCAAAAGGG TGGCAGTGCC GAACGGACAA 
CCGCCAAGCG CCGCCCGCTA CATGCCTCGG GAGGTGCCGC CGCGATTCCG TTGCCAGCAG 
GACCACAAAG TGTTACTAAA ACGTGGGCAG CCCCCTCCGC CGTCCTGCAT GCTCCTTGGG 
GGCGGAGCAG GGCCTCCTCC CTCCACAGCA CCTGGAGCAA ACTCAAACAA CGCACAAGTG 
ACAGGAACGC TGCTGCAGAG TGACAGTGGG ACTGCACCAG AGTCAACCCT TGGAGGTGCT 
GCTGCTTCAA ATTATGCAAA TTCCACTTGG GGCCCGGGAG CCTCCTCCAA CAACGGCGCC 
TCCCCCAACC CAATTCACAC CTGGGACAAG GTGATTGTAG ACGGGTCTGA CGTGGAAGAG 
TGGCCTTGTA TTGCCAGCAA AGACACTGAG TCTTCTTCCG AAAACACCAC CGATAACAAC 
AGTGCCTCGA ACCCTGGCTC TGAGAAGAGC ACTCTGCCAG GAAGCACCAC TAGTAACAAA 
GGAAAAGGGA GCCAGTGCCA GTCTGCAAGT TCTGGGAACG AATGTAATCT TGGGGTCTGG 
AAATCTGACC CTAAGACTAA ATCTGTTCAA TCTTCCAACT CTACAACAGA GAGCAACAAT 
GGACTAGGAA ATTGGAGGAA TGTGAGTGGT CAGGATAGAA TTGGACCTGG CTCTGGCTTC 
AGCAACTTTA ACCCAAATAG CAACCCATCT GCCTGGCCAG CGCTGGTCCA AGAAGGAAAT 
TCTAGGAAAG GGGCATTGGA AACGGATACT AGTAATTCCA GTGCACAGGT TAGCACAGTA 
GGTCAGGCAT CCAGGGAACA GCAGTCAAAG ATGGAAAATG CGGGTGTTAA TTTTGTTGTC 
TCTGGCAGAG AACAGGCTCA GATTCATAAC ACTGATGGAC CAAAAAATGG AAACACTAAC 
TCCTTGAACT TAAGTTCACC AAACCCCATG GAGAATAAGG GAATGCCCTT TGGAATGGGC 
TTGGGGAACG CCTCCAGGAG CACTGATGCC CCTTCACAAA GCACTGGAGA TCGAAAGACT 
GGGAGTGTTG GATCTTGGGG TGCAGCTAGG GGGCCTTCTG GAACTGACAC AGCCTCTGGA 
CAAAGCAATT CTGGAAACAA TGGGAACAAT GGAAAAGATA GAGAGGACTC CTGGAAAGGA 
GCTTCTGTTC AGAAATCATC TGGGTCAAAA AATGACTCTT GGGACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGATGAGTTG 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACAGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCATAA TTCTGGCCGT CGGTCATACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TACAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACTGTGT GGGATATTGA AGAGGTGCCA 
AGGCCTGAGG GGAAATCGGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAACTCAG GGGGCTGGGG AGATGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGGGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGCGGACGAC CAGATGAAAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GGGGACGCCA GCCCAATCAA 
GGATGGACTT CTGGGAAGAA TGGTTGGGGA GAGGAAGTTG ATCAGGCGAA AAACAGCAGT 
TGGGAAAGTT CTGCAAGCAA ACCTGTGTCT GGATGGGGAG AAGGAGGGCA GAATGAAATT 
GGAACTTGGG GGAACAGCGG GAATGCAAGC CTAGCTTCCA AAGGTGGGTG GGAAGATTGC 
AAAAGGTCTC CAGCGTGGAC TGAGACTGGC AGACAGCCCA ATTCCTGGCA TAAACAGCAC 
CAACAGCAGC AGCAGCCACA GCAGCCACCG CCACCACAAC CAGAGGCTTC TGGTTCATGG 
GGAGGCCCCC CACCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGT 
GGGCCACAGC CAGTAACCCC TAAGGACGAG GAACCCAGTG GTTGGGAAGA GCCATCCCCA 
CAGTCAATTA GTCGGAAAAT GGACATCGAT GATGGCACTT CAGCATGGGG AGACCCTAAC 
AGCTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAACCAA ACCTGCCTAC CCCGATGACC AGTAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCACCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AAATGGATGA CACGGGAGCA TCGACCACGG GCTGGGGGAA TGCACCCTCC 
AACGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGACGGCTG GGGGGAGAGT 
GACGGTCCAG TGACGGGAAC TCGCCATCCC AGCTGGGAGG AGGAGGATGA TGGAGGAGTC 
TGGAACACCG CCGGCTCTCA GGGAAGTGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AGCAAATGAA GTGCTCACTG AAAGGAGGAA ACAATGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAATCCAGGC 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGACGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTCGAGAA GCTGACTTTG 
CCTTTCTCCA ATCAAGATGG GTGCCTTGGG GATGAGGCTC CCTGCTCTCC CTTCTCCCCT 
TCTCCCAGCT ACAAGCTGTC TCCCTCTGGT TCCACACTAC CCAACGTCAG CCTTGGAGCA 
ATCGGCACAG GGCTCAACCC CCAAAACTTC GCTGCTAGAC AAGGTGGCAA TCATGGTTTG 
TTTGGAAATA GCACAGCACA ATCGAGAGGT ATGCACACAC CAGTGCAGCC ACTAAATTCT 
TCTCCTAATC TCCGGGCGCA AGTGCCTCCC CAGTTTATTT CCCCCCAGGT TTCTGCCTCC 
ATGCTCAAGC AGTTTCCCAA CAGTGGCCTG AATCCAGGTC TTTTCAATGT GGGGCCCCAG 
TTATCTCCTC AACAGATTGC CATGCTGAGC CAGCTTCCAC AAATTCCCCA GTTTCAGTTG 
GCATGTCAGC TTCTCCTGCA GCAGCAGCAA CAGCAGCAGT TGCTACAGAA CCAGAGGAAG 
CTTTCTCAAG CTGTGCGCCA GCAGCAAGAG CAGCAGCTGG CTCGAATGGT GAGCGCTCTC 
CAGCAACAGC AGCAACAGCA GCAGCAGCAG CAGCAGAGGC AGCCTGGCAT GAAGCACTCA 
CCCTCTCATC CTGTTGGGCC CAAGCCACAT CTGGACAACA TGGTACCCAA CGCTCTGAAT 
GTGGGGCTCC CAGACCTTCA AACCAAAGGG CCAATACCTG GATATGGTTC TGGCTTCAGC 
TCTGGCGGCA TGGACTATGG CATGGTTGGT GGGAAGGAGG CTGGAACAGA GTCTCGCTTT 
AAACAGTGGA CCTCCATGAT GGAGGGACTG CCCTCTGTAG CCACACAGGA AGCCAATATG 
CACAAAAATG GCGCTATTGT GGCCCCTGGT AAGACTCGAG GAGGGTCACC GTACAACCAG 
TTTGATATCA TCCCGAGTGA CACACTGGGT GGCCATACGG GTCCTGCTGG TGATAGCTGG 
TTACCTGCCA AATCTCCACC AACAAATAAA ATCGGAAGTA AATCCAGCAA TGCCAGTTGG 
CCTCCAGAAT TCCAACCAGG AGTGCCATGG AAAGGTATCC AAAACATTGA CCCTGAATCT 
GACCCCTATG TCACCCCAGG AAGTGTACTG GGGGGTACAG CCACATCTCC CATTGTAGAT 
ACTGACCACC AACTTCTGCG GGATAACACC ACAGGGTCTA ATTCTTCCCT CAACACCTCG 
CTGCCTTCAC CTGGTGCCTG GCCCTACAGT GCCTCTGACA ACTCCTTTAC CAACGTTCAT 
AGCACTTCAG CAAAGTTCCC TGATTACAAA TCAACATGGT CCCCAGATCC CATAGGACAC 
AACCCCACTC ATCTCTCCAA CAAGATGTGG AAAAACCATA TTTCCTCAAG GAACACTACA 
CCGCTGCCCC GCCCACCTCC CGGTCTGACC AACCCCAAAC CATCATCTCC CTGGAGCAGC 
ACAGCACCCC GATCAGTCAG GGGGTGGGGG ACACAGGACT CACGGCTTGC CTCGGCCTCT 
ACCTGGAGTG ATGGTGGTTC AGTTCGTCCC AGTTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATTG ATGGGTCAAC GTTGAGAACC ATCTGCATGC AGCATGGCCC ACTGCTGACA 
TTCCATCTGA ACCTAACCCA GGGCACTGCC CTGATCCGAT ACAGCACCAA ACAGGAGGCG 
GCCAAGGCCC AAACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTGGCTGAG 
TTTGCCACCG ATGATGAAGT TAGCCGCTTT CTGGCACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCGC CGCAGGTTGG CAGTCGCTGG AGACCGGCCA GACCCAGTCA 
GATCCCGTCG GACCTGCTTT GAATCTTTTT GGTGGGTCCA CAGGGCTCGG GCAATGGAGC 
AGCAGTGCTG GTGGCAGCAG CGGGGCCGAT CTTGCTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CTAGCTTGTG GGGAGTCCCA GCTGTGGAAG ATCCCCATAG GATGGGCAGC 
CCTGCTCCCT TACTACCTGG TGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280095.1
CDS	347..6040
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKAPDILKHAVNHTLILGCLKFGLQEAVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424387.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424387.1

ORF Insert Sequence:

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGCACCTGA CATTCTGAAG 
CATGCTGTGA ACCACACACT GATTCTGGGA TGTCTTAAGT TTGGACTTCA AGAAGCAGTG 
CCCGAAGTGA CGAAACCAAG TTTAAGCCAA CCAACGGCCG CCAGCCCAAT TGGCAGCTCT 
CCATCGCCAC CAGTCAATGG TGGCAACAAT GCCAAAAGGG TGGCAGTGCC GAACGGACAA 
CCGCCAAGCG CCGCCCGCTA CATGCCTCGG GAGGTGCCGC CGCGATTCCG TTGCCAGCAG 
GACCACAAAG TGTTACTAAA ACGTGGGCAG CCCCCTCCGC CGTCCTGCAT GCTCCTTGGG 
GGCGGAGCAG GGCCTCCTCC CTCCACAGCA CCTGGAGCAA ACTCAAACAA CGCACAAGTG 
ACAGGAACGC TGCTGCAGAG TGACAGTGGG ACTGCACCAG AGTCAACCCT TGGAGGTGCT 
GCTGCTTCAA ATTATGCAAA TTCCACTTGG GGCCCGGGAG CCTCCTCCAA CAACGGCGCC 
TCCCCCAACC CAATTCACAC CTGGGACAAG GTGATTGTAG ACGGGTCTGA CGTGGAAGAG 
TGGCCTTGTA TTGCCAGCAA AGACACTGAG TCTTCTTCCG AAAACACCAC CGATAACAAC 
AGTGCCTCGA ACCCTGGCTC TGAGAAGAGC ACTCTGCCAG GAAGCACCAC TAGTAACAAA 
GGAAAAGGGA GCCAGTGCCA GTCTGCAAGT TCTGGGAACG AATGTAATCT TGGGGTCTGG 
AAATCTGACC CTAAGACTAA ATCTGTTCAA TCTTCCAACT CTACAACAGA GAGCAACAAT 
GGACTAGGAA ATTGGAGGAA TGTGAGTGGT CAGGATAGAA TTGGACCTGG CTCTGGCTTC 
AGCAACTTTA ACCCAAATAG CAACCCATCT GCCTGGCCAG CGCTGGTCCA AGAAGGAAAT 
TCTAGGAAAG GGGCATTGGA AACGGATACT AGTAATTCCA GTGCACAGGT TAGCACAGTA 
GGTCAGGCAT CCAGGGAACA GCAGTCAAAG ATGGAAAATG CGGGTGTTAA TTTTGTTGTC 
TCTGGCAGAG AACAGGCTCA GATTCATAAC ACTGATGGAC CAAAAAATGG AAACACTAAC 
TCCTTGAACT TAAGTTCACC AAACCCCATG GAGAATAAGG GAATGCCCTT TGGAATGGGC 
TTGGGGAACG CCTCCAGGAG CACTGATGCC CCTTCACAAA GCACTGGAGA TCGAAAGACT 
GGGAGTGTTG GATCTTGGGG TGCAGCTAGG GGGCCTTCTG GAACTGACAC AGCCTCTGGA 
CAAAGCAATT CTGGAAACAA TGGGAACAAT GGAAAAGATA GAGAGGACTC CTGGAAAGGA 
GCTTCTGTTC AGAAATCATC TGGGTCAAAA AATGACTCTT GGGACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGATGAGTTG 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACAGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCATAA TTCTGGCCGT CGGTCATACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TACAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACTGTGT GGGATATTGA AGAGGTGCCA 
AGGCCTGAGG GGAAATCGGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAACTCAG GGGGCTGGGG AGATGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGGGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGCGGACGAC CAGATGAAAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GGGGACGCCA GCCCAATCAA 
GGATGGACTT CTGGGAAGAA TGGTTGGGGA GAGGAAGTTG ATCAGGCGAA AAACAGCAGT 
TGGGAAAGTT CTGCAAGCAA ACCTGTGTCT GGATGGGGAG AAGGAGGGCA GAATGAAATT 
GGAACTTGGG GGAACAGCGG GAATGCAAGC CTAGCTTCCA AAGGTGGGTG GGAAGATTGC 
AAAAGGTCTC CAGCGTGGAC TGAGACTGGC AGACAGCCCA ATTCCTGGCA TAAACAGCAC 
CAACAGCAGC AGCAGCCACA GCAGCCACCG CCACCACAAC CAGAGGCTTC TGGTTCATGG 
GGAGGCCCCC CACCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGT 
GGGCCACAGC CAGTAACCCC TAAGGACGAG GAACCCAGTG GTTGGGAAGA GCCATCCCCA 
CAGTCAATTA GTCGGAAAAT GGACATCGAT GATGGCACTT CAGCATGGGG AGACCCTAAC 
AGCTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAACCAA ACCTGCCTAC CCCGATGACC AGTAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCACCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AAATGGATGA CACGGGAGCA TCGACCACGG GCTGGGGGAA TGCACCCTCC 
AACGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGACGGCTG GGGGGAGAGT 
GACGGTCCAG TGACGGGAAC TCGCCATCCC AGCTGGGAGG AGGAGGATGA TGGAGGAGTC 
TGGAACACCG CCGGCTCTCA GGGAAGTGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AGCAAATGAA GTGCTCACTG AAAGGAGGAA ACAATGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAATCCAGGC 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGACGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTCGAGAA GCTGACTTTG 
CCTTTCTCCA ATCAAGATGG GTGCCTTGGG GATGAGGCTC CCTGCTCTCC CTTCTCCCCT 
TCTCCCAGCT ACAAGCTGTC TCCCTCTGGT TCCACACTAC CCAACGTCAG CCTTGGAGCA 
ATCGGCACAG GGCTCAACCC CCAAAACTTC GCTGCTAGAC AAGGTGGCAA TCATGGTTTG 
TTTGGAAATA GCACAGCACA ATCGAGAGGT ATGCACACAC CAGTGCAGCC ACTAAATTCT 
TCTCCTAATC TCCGGGCGCA AGTGCCTCCC CAGTTTATTT CCCCCCAGGT TTCTGCCTCC 
ATGCTCAAGC AGTTTCCCAA CAGTGGCCTG AATCCAGGTC TTTTCAATGT GGGGCCCCAG 
TTATCTCCTC AACAGATTGC CATGCTGAGC CAGCTTCCAC AAATTCCCCA GTTTCAGTTG 
GCATGTCAGC TTCTCCTGCA GCAGCAGCAA CAGCAGCAGT TGCTACAGAA CCAGAGGAAG 
CTTTCTCAAG CTGTGCGCCA GCAGCAAGAG CAGCAGCTGG CTCGAATGGT GAGCGCTCTC 
CAGCAACAGC AGCAACAGCA GCAGCAGCAG CAGCAGAGGC AGCCTGGCAT GAAGCACTCA 
CCCTCTCATC CTGTTGGGCC CAAGCCACAT CTGGACAACA TGGTACCCAA CGCTCTGAAT 
GTGGGGCTCC CAGACCTTCA AACCAAAGGG CCAATACCTG GATATGGTTC TGGCTTCAGC 
TCTGGCGGCA TGGACTATGG CATGGTTGGT GGGAAGGAGG CTGGAACAGA GTCTCGCTTT 
AAACAGTGGA CCTCCATGAT GGAGGGACTG CCCTCTGTAG CCACACAGGA AGCCAATATG 
CACAAAAATG GCGCTATTGT GGCCCCTGGT AAGACTCGAG GAGGGTCACC GTACAACCAG 
TTTGATATCA TCCCGAGTGA CACACTGGGT GGCCATACGG GTCCTGCTGG TGATAGCTGG 
TTACCTGCCA AATCTCCACC AACAAATAAA ATCGGAAGTA AATCCAGCAA TGCCAGTTGG 
CCTCCAGAAT TCCAACCAGG AGTGCCATGG AAAGGTATCC AAAACATTGA CCCTGAATCT 
GACCCCTATG TCACCCCAGG AAGTGTACTG GGGGGTACAG CCACATCTCC CATTGTAGAT 
ACTGACCACC AACTTCTGCG GGATAACACC ACAGGGTCTA ATTCTTCCCT CAACACCTCG 
CTGCCTTCAC CTGGTGCCTG GCCCTACAGT GCCTCTGACA ACTCCTTTAC CAACGTTCAT 
AGCACTTCAG CAAAGTTCCC TGATTACAAA TCAACATGGT CCCCAGATCC CATAGGACAC 
AACCCCACTC ATCTCTCCAA CAAGATGTGG AAAAACCATA TTTCCTCAAG GAACACTACA 
CCGCTGCCCC GCCCACCTCC CGGTCTGACC AACCCCAAAC CATCATCTCC CTGGAGCAGC 
ACAGCACCCC GATCAGTCAG GGGGTGGGGG ACACAGGACT CACGGCTTGC CTCGGCCTCT 
ACCTGGAGTG ATGGTGGTTC AGTTCGTCCC AGTTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATTG ATGGGTCAAC GTTGAGAACC ATCTGCATGC AGCATGGCCC ACTGCTGACA 
TTCCATCTGA ACCTAACCCA GGGCACTGCC CTGATCCGAT ACAGCACCAA ACAGGAGGCG 
GCCAAGGCCC AAACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTGGCTGAG 
TTTGCCACCG ATGATGAAGT TAGCCGCTTT CTGGCACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCGC CGCAGGTTGG CAGTCGCTGG AGACCGGCCA GACCCAGTCA 
GATCCCGTCG GACCTGCTTT GAATCTTTTT GGTGGGTCCA CAGGGCTCGG GCAATGGAGC 
AGCAGTGCTG GTGGCAGCAG CGGGGCCGAT CTTGCTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CTAGCTTGTG GGGAGTCCCA GCTGTGGAAG ATCCCCATAG GATGGGCAGC 
CCTGCTCCCT TACTACCTGG TGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424396.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280104.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X12, mRNA.

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CloneID	OCe291427
Clone ID Related Accession (Same CDS sequence)	XM_022424387.1 , XM_022424396.1
Accession Version	XM_022424396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGCACCTGA CATTCTGAAG 
CATGCTGTGA ACCACACACT GATTCTGGGA TGTCTTAAGT TTGGACTTCA AGAAGCAGTG 
CCCGAAGTGA CGAAACCAAG TTTAAGCCAA CCAACGGCCG CCAGCCCAAT TGGCAGCTCT 
CCATCGCCAC CAGTCAATGG TGGCAACAAT GCCAAAAGGG TGGCAGTGCC GAACGGACAA 
CCGCCAAGCG CCGCCCGCTA CATGCCTCGG GAGGTGCCGC CGCGATTCCG TTGCCAGCAG 
GACCACAAAG TGTTACTAAA ACGTGGGCAG CCCCCTCCGC CGTCCTGCAT GCTCCTTGGG 
GGCGGAGCAG GGCCTCCTCC CTCCACAGCA CCTGGAGCAA ACTCAAACAA CGCACAAGTG 
ACAGGAACGC TGCTGCAGAG TGACAGTGGG ACTGCACCAG AGTCAACCCT TGGAGGTGCT 
GCTGCTTCAA ATTATGCAAA TTCCACTTGG GGCCCGGGAG CCTCCTCCAA CAACGGCGCC 
TCCCCCAACC CAATTCACAC CTGGGACAAG GTGATTGTAG ACGGGTCTGA CGTGGAAGAG 
TGGCCTTGTA TTGCCAGCAA AGACACTGAG TCTTCTTCCG AAAACACCAC CGATAACAAC 
AGTGCCTCGA ACCCTGGCTC TGAGAAGAGC ACTCTGCCAG GAAGCACCAC TAGTAACAAA 
GGAAAAGGGA GCCAGTGCCA GTCTGCAAGT TCTGGGAACG AATGTAATCT TGGGGTCTGG 
AAATCTGACC CTAAGACTAA ATCTGTTCAA TCTTCCAACT CTACAACAGA GAGCAACAAT 
GGACTAGGAA ATTGGAGGAA TGTGAGTGGT CAGGATAGAA TTGGACCTGG CTCTGGCTTC 
AGCAACTTTA ACCCAAATAG CAACCCATCT GCCTGGCCAG CGCTGGTCCA AGAAGGAAAT 
TCTAGGAAAG GGGCATTGGA AACGGATACT AGTAATTCCA GTGCACAGGT TAGCACAGTA 
GGTCAGGCAT CCAGGGAACA GCAGTCAAAG ATGGAAAATG CGGGTGTTAA TTTTGTTGTC 
TCTGGCAGAG AACAGGCTCA GATTCATAAC ACTGATGGAC CAAAAAATGG AAACACTAAC 
TCCTTGAACT TAAGTTCACC AAACCCCATG GAGAATAAGG GAATGCCCTT TGGAATGGGC 
TTGGGGAACG CCTCCAGGAG CACTGATGCC CCTTCACAAA GCACTGGAGA TCGAAAGACT 
GGGAGTGTTG GATCTTGGGG TGCAGCTAGG GGGCCTTCTG GAACTGACAC AGCCTCTGGA 
CAAAGCAATT CTGGAAACAA TGGGAACAAT GGAAAAGATA GAGAGGACTC CTGGAAAGGA 
GCTTCTGTTC AGAAATCATC TGGGTCAAAA AATGACTCTT GGGACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGATGAGTTG 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACAGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCATAA TTCTGGCCGT CGGTCATACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TACAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACTGTGT GGGATATTGA AGAGGTGCCA 
AGGCCTGAGG GGAAATCGGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAACTCAG GGGGCTGGGG AGATGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGGGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGCGGACGAC CAGATGAAAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GGGGACGCCA GCCCAATCAA 
GGATGGACTT CTGGGAAGAA TGGTTGGGGA GAGGAAGTTG ATCAGGCGAA AAACAGCAGT 
TGGGAAAGTT CTGCAAGCAA ACCTGTGTCT GGATGGGGAG AAGGAGGGCA GAATGAAATT 
GGAACTTGGG GGAACAGCGG GAATGCAAGC CTAGCTTCCA AAGGTGGGTG GGAAGATTGC 
AAAAGGTCTC CAGCGTGGAC TGAGACTGGC AGACAGCCCA ATTCCTGGCA TAAACAGCAC 
CAACAGCAGC AGCAGCCACA GCAGCCACCG CCACCACAAC CAGAGGCTTC TGGTTCATGG 
GGAGGCCCCC CACCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGT 
GGGCCACAGC CAGTAACCCC TAAGGACGAG GAACCCAGTG GTTGGGAAGA GCCATCCCCA 
CAGTCAATTA GTCGGAAAAT GGACATCGAT GATGGCACTT CAGCATGGGG AGACCCTAAC 
AGCTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAACCAA ACCTGCCTAC CCCGATGACC AGTAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCACCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AAATGGATGA CACGGGAGCA TCGACCACGG GCTGGGGGAA TGCACCCTCC 
AACGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGACGGCTG GGGGGAGAGT 
GACGGTCCAG TGACGGGAAC TCGCCATCCC AGCTGGGAGG AGGAGGATGA TGGAGGAGTC 
TGGAACACCG CCGGCTCTCA GGGAAGTGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AGCAAATGAA GTGCTCACTG AAAGGAGGAA ACAATGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAATCCAGGC 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGACGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTCGAGAA GCTGACTTTG 
CCTTTCTCCA ATCAAGATGG GTGCCTTGGG GATGAGGCTC CCTGCTCTCC CTTCTCCCCT 
TCTCCCAGCT ACAAGCTGTC TCCCTCTGGT TCCACACTAC CCAACGTCAG CCTTGGAGCA 
ATCGGCACAG GGCTCAACCC CCAAAACTTC GCTGCTAGAC AAGGTGGCAA TCATGGTTTG 
TTTGGAAATA GCACAGCACA ATCGAGAGGT ATGCACACAC CAGTGCAGCC ACTAAATTCT 
TCTCCTAATC TCCGGGCGCA AGTGCCTCCC CAGTTTATTT CCCCCCAGGT TTCTGCCTCC 
ATGCTCAAGC AGTTTCCCAA CAGTGGCCTG AATCCAGGTC TTTTCAATGT GGGGCCCCAG 
TTATCTCCTC AACAGATTGC CATGCTGAGC CAGCTTCCAC AAATTCCCCA GTTTCAGTTG 
GCATGTCAGC TTCTCCTGCA GCAGCAGCAA CAGCAGCAGT TGCTACAGAA CCAGAGGAAG 
CTTTCTCAAG CTGTGCGCCA GCAGCAAGAG CAGCAGCTGG CTCGAATGGT GAGCGCTCTC 
CAGCAACAGC AGCAACAGCA GCAGCAGCAG CAGCAGAGGC AGCCTGGCAT GAAGCACTCA 
CCCTCTCATC CTGTTGGGCC CAAGCCACAT CTGGACAACA TGGTACCCAA CGCTCTGAAT 
GTGGGGCTCC CAGACCTTCA AACCAAAGGG CCAATACCTG GATATGGTTC TGGCTTCAGC 
TCTGGCGGCA TGGACTATGG CATGGTTGGT GGGAAGGAGG CTGGAACAGA GTCTCGCTTT 
AAACAGTGGA CCTCCATGAT GGAGGGACTG CCCTCTGTAG CCACACAGGA AGCCAATATG 
CACAAAAATG GCGCTATTGT GGCCCCTGGT AAGACTCGAG GAGGGTCACC GTACAACCAG 
TTTGATATCA TCCCGAGTGA CACACTGGGT GGCCATACGG GTCCTGCTGG TGATAGCTGG 
TTACCTGCCA AATCTCCACC AACAAATAAA ATCGGAAGTA AATCCAGCAA TGCCAGTTGG 
CCTCCAGAAT TCCAACCAGG AGTGCCATGG AAAGGTATCC AAAACATTGA CCCTGAATCT 
GACCCCTATG TCACCCCAGG AAGTGTACTG GGGGGTACAG CCACATCTCC CATTGTAGAT 
ACTGACCACC AACTTCTGCG GGATAACACC ACAGGGTCTA ATTCTTCCCT CAACACCTCG 
CTGCCTTCAC CTGGTGCCTG GCCCTACAGT GCCTCTGACA ACTCCTTTAC CAACGTTCAT 
AGCACTTCAG CAAAGTTCCC TGATTACAAA TCAACATGGT CCCCAGATCC CATAGGACAC 
AACCCCACTC ATCTCTCCAA CAAGATGTGG AAAAACCATA TTTCCTCAAG GAACACTACA 
CCGCTGCCCC GCCCACCTCC CGGTCTGACC AACCCCAAAC CATCATCTCC CTGGAGCAGC 
ACAGCACCCC GATCAGTCAG GGGGTGGGGG ACACAGGACT CACGGCTTGC CTCGGCCTCT 
ACCTGGAGTG ATGGTGGTTC AGTTCGTCCC AGTTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATTG ATGGGTCAAC GTTGAGAACC ATCTGCATGC AGCATGGCCC ACTGCTGACA 
TTCCATCTGA ACCTAACCCA GGGCACTGCC CTGATCCGAT ACAGCACCAA ACAGGAGGCG 
GCCAAGGCCC AAACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTGGCTGAG 
TTTGCCACCG ATGATGAAGT TAGCCGCTTT CTGGCACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCGC CGCAGGTTGG CAGTCGCTGG AGACCGGCCA GACCCAGTCA 
GATCCCGTCG GACCTGCTTT GAATCTTTTT GGTGGGTCCA CAGGGCTCGG GCAATGGAGC 
AGCAGTGCTG GTGGCAGCAG CGGGGCCGAT CTTGCTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CTAGCTTGTG GGGAGTCCCA GCTGTGGAAG ATCCCCATAG GATGGGCAGC 
CCTGCTCCCT TACTACCTGG TGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280104.1
CDS	207..5900
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKAPDILKHAVNHTLILGCLKFGLQEAVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424396.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424396.1

ORF Insert Sequence:

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGCACCTGA CATTCTGAAG 
CATGCTGTGA ACCACACACT GATTCTGGGA TGTCTTAAGT TTGGACTTCA AGAAGCAGTG 
CCCGAAGTGA CGAAACCAAG TTTAAGCCAA CCAACGGCCG CCAGCCCAAT TGGCAGCTCT 
CCATCGCCAC CAGTCAATGG TGGCAACAAT GCCAAAAGGG TGGCAGTGCC GAACGGACAA 
CCGCCAAGCG CCGCCCGCTA CATGCCTCGG GAGGTGCCGC CGCGATTCCG TTGCCAGCAG 
GACCACAAAG TGTTACTAAA ACGTGGGCAG CCCCCTCCGC CGTCCTGCAT GCTCCTTGGG 
GGCGGAGCAG GGCCTCCTCC CTCCACAGCA CCTGGAGCAA ACTCAAACAA CGCACAAGTG 
ACAGGAACGC TGCTGCAGAG TGACAGTGGG ACTGCACCAG AGTCAACCCT TGGAGGTGCT 
GCTGCTTCAA ATTATGCAAA TTCCACTTGG GGCCCGGGAG CCTCCTCCAA CAACGGCGCC 
TCCCCCAACC CAATTCACAC CTGGGACAAG GTGATTGTAG ACGGGTCTGA CGTGGAAGAG 
TGGCCTTGTA TTGCCAGCAA AGACACTGAG TCTTCTTCCG AAAACACCAC CGATAACAAC 
AGTGCCTCGA ACCCTGGCTC TGAGAAGAGC ACTCTGCCAG GAAGCACCAC TAGTAACAAA 
GGAAAAGGGA GCCAGTGCCA GTCTGCAAGT TCTGGGAACG AATGTAATCT TGGGGTCTGG 
AAATCTGACC CTAAGACTAA ATCTGTTCAA TCTTCCAACT CTACAACAGA GAGCAACAAT 
GGACTAGGAA ATTGGAGGAA TGTGAGTGGT CAGGATAGAA TTGGACCTGG CTCTGGCTTC 
AGCAACTTTA ACCCAAATAG CAACCCATCT GCCTGGCCAG CGCTGGTCCA AGAAGGAAAT 
TCTAGGAAAG GGGCATTGGA AACGGATACT AGTAATTCCA GTGCACAGGT TAGCACAGTA 
GGTCAGGCAT CCAGGGAACA GCAGTCAAAG ATGGAAAATG CGGGTGTTAA TTTTGTTGTC 
TCTGGCAGAG AACAGGCTCA GATTCATAAC ACTGATGGAC CAAAAAATGG AAACACTAAC 
TCCTTGAACT TAAGTTCACC AAACCCCATG GAGAATAAGG GAATGCCCTT TGGAATGGGC 
TTGGGGAACG CCTCCAGGAG CACTGATGCC CCTTCACAAA GCACTGGAGA TCGAAAGACT 
GGGAGTGTTG GATCTTGGGG TGCAGCTAGG GGGCCTTCTG GAACTGACAC AGCCTCTGGA 
CAAAGCAATT CTGGAAACAA TGGGAACAAT GGAAAAGATA GAGAGGACTC CTGGAAAGGA 
GCTTCTGTTC AGAAATCATC TGGGTCAAAA AATGACTCTT GGGACAATAA CAGGTCTACG 
GGTGGGTCCT GGAACTTTGG CCCTCAGGAC TCTAATGACA ACAAATGGGG TGAAGGGAAC 
AAAATGACAT CTGGGGTCTC TCAGGGAGAA TGGAAACAGC CGACTGGGTC TGATGAGTTG 
AAAATTGGAG AATGGAGTGG TCCAAACCAA CCAAATTCTA GCACTGGAGC ATGGGACAAT 
CAAAAGGGCC ACCCCCTCCC TGAAAACCAA GGCAATGCCC AGGCTCCCTG TTGGGGAAGA 
TCTTCCAGCT CCACAGGAAG TGAAGTTGGA GGTCAAAGCA CTGGAAGCAA CCACAAAGCA 
GGAAGTAGTG ACAGTCATAA TTCTGGCCGT CGGTCATACC GGCCCACACA TCCTGATTGT 
CAGGCTGTCT TACAGACTCT TTTGAGCCGA ACTGATTTGG ACCCCAGGGT GCTCTCAAAC 
ACTGGCTGGG GCCAAACTCA AATTAAGCAG GACACTGTGT GGGATATTGA AGAGGTGCCA 
AGGCCTGAGG GGAAATCGGA CAAAGGAACT GAGGGGTGGG AGAGCGCTGC CACACAGACC 
AAGAACTCAG GGGGCTGGGG AGATGCACCC AGCCAAAGCA ATCAAATGAA GTCTGGATGG 
GGGGAGCTCT CAGCCTCTAC AGAGTGGAAA GACCCCAAGA ACACAGGAGG CTGGAATGAC 
TATAAGAACA ACAACTCTTC CAACTGGGGA GGCGGACGAC CAGATGAAAA GACATCTTCC 
TCTTGGAATG AGAACTCCAG CAAGGATCAG GGGTGGGGAG GGGGACGCCA GCCCAATCAA 
GGATGGACTT CTGGGAAGAA TGGTTGGGGA GAGGAAGTTG ATCAGGCGAA AAACAGCAGT 
TGGGAAAGTT CTGCAAGCAA ACCTGTGTCT GGATGGGGAG AAGGAGGGCA GAATGAAATT 
GGAACTTGGG GGAACAGCGG GAATGCAAGC CTAGCTTCCA AAGGTGGGTG GGAAGATTGC 
AAAAGGTCTC CAGCGTGGAC TGAGACTGGC AGACAGCCCA ATTCCTGGCA TAAACAGCAC 
CAACAGCAGC AGCAGCCACA GCAGCCACCG CCACCACAAC CAGAGGCTTC TGGTTCATGG 
GGAGGCCCCC CACCACCACC TCCAGGCAAC GGACGACCTT CCAATTCCAA CTGGAGCAGT 
GGGCCACAGC CAGTAACCCC TAAGGACGAG GAACCCAGTG GTTGGGAAGA GCCATCCCCA 
CAGTCAATTA GTCGGAAAAT GGACATCGAT GATGGCACTT CAGCATGGGG AGACCCTAAC 
AGCTATAACT ACAAGAATGT GAATCTGTGG GATAAGAATT CCCAAGGGGG CCCAGCACCT 
CGAGAACCAA ACCTGCCTAC CCCGATGACC AGTAAATCAG CATCAGTCTG GAGCAAAAGC 
ACACCACCTG CTCCAGATAA TGGTACTTCA GCTTGGGGTG AGCCAAACGA AAGCAGTCCT 
GGGTGGGGCG AAATGGATGA CACGGGAGCA TCGACCACGG GCTGGGGGAA TGCACCCTCC 
AACGCTCCAA ATGCCATGAA ACCTAATTCC AAATCTATGC AAGACGGCTG GGGGGAGAGT 
GACGGTCCAG TGACGGGAAC TCGCCATCCC AGCTGGGAGG AGGAGGATGA TGGAGGAGTC 
TGGAACACCG CCGGCTCTCA GGGAAGTGCT TCCTCCCACA ACTCAGCAAG CTGGGGACAA 
GGAGGAAAGA AGCAAATGAA GTGCTCACTG AAAGGAGGAA ACAATGATTC ATGGATGAAT 
CCTCTTGCCA AACAGTTTTC AAATATGGGA TTGCTGAGTC AAACTGAAGA TAATCCAGGC 
AGCAAAATGG ATTTGTCTGT AGGGAGCCTT CCAGATAAAA AATTTGACGT GGACAAGCGA 
GCAATGAATC TCGGGGATTT TAATGATATC ATGAGGAAGG ATCGATCTGG GTTCCGTCCA 
CCTAATTCCA AAGACATGGG AACCACAGAT AGTGGGCCTT ATTTCGAGAA GCTGACTTTG 
CCTTTCTCCA ATCAAGATGG GTGCCTTGGG GATGAGGCTC CCTGCTCTCC CTTCTCCCCT 
TCTCCCAGCT ACAAGCTGTC TCCCTCTGGT TCCACACTAC CCAACGTCAG CCTTGGAGCA 
ATCGGCACAG GGCTCAACCC CCAAAACTTC GCTGCTAGAC AAGGTGGCAA TCATGGTTTG 
TTTGGAAATA GCACAGCACA ATCGAGAGGT ATGCACACAC CAGTGCAGCC ACTAAATTCT 
TCTCCTAATC TCCGGGCGCA AGTGCCTCCC CAGTTTATTT CCCCCCAGGT TTCTGCCTCC 
ATGCTCAAGC AGTTTCCCAA CAGTGGCCTG AATCCAGGTC TTTTCAATGT GGGGCCCCAG 
TTATCTCCTC AACAGATTGC CATGCTGAGC CAGCTTCCAC AAATTCCCCA GTTTCAGTTG 
GCATGTCAGC TTCTCCTGCA GCAGCAGCAA CAGCAGCAGT TGCTACAGAA CCAGAGGAAG 
CTTTCTCAAG CTGTGCGCCA GCAGCAAGAG CAGCAGCTGG CTCGAATGGT GAGCGCTCTC 
CAGCAACAGC AGCAACAGCA GCAGCAGCAG CAGCAGAGGC AGCCTGGCAT GAAGCACTCA 
CCCTCTCATC CTGTTGGGCC CAAGCCACAT CTGGACAACA TGGTACCCAA CGCTCTGAAT 
GTGGGGCTCC CAGACCTTCA AACCAAAGGG CCAATACCTG GATATGGTTC TGGCTTCAGC 
TCTGGCGGCA TGGACTATGG CATGGTTGGT GGGAAGGAGG CTGGAACAGA GTCTCGCTTT 
AAACAGTGGA CCTCCATGAT GGAGGGACTG CCCTCTGTAG CCACACAGGA AGCCAATATG 
CACAAAAATG GCGCTATTGT GGCCCCTGGT AAGACTCGAG GAGGGTCACC GTACAACCAG 
TTTGATATCA TCCCGAGTGA CACACTGGGT GGCCATACGG GTCCTGCTGG TGATAGCTGG 
TTACCTGCCA AATCTCCACC AACAAATAAA ATCGGAAGTA AATCCAGCAA TGCCAGTTGG 
CCTCCAGAAT TCCAACCAGG AGTGCCATGG AAAGGTATCC AAAACATTGA CCCTGAATCT 
GACCCCTATG TCACCCCAGG AAGTGTACTG GGGGGTACAG CCACATCTCC CATTGTAGAT 
ACTGACCACC AACTTCTGCG GGATAACACC ACAGGGTCTA ATTCTTCCCT CAACACCTCG 
CTGCCTTCAC CTGGTGCCTG GCCCTACAGT GCCTCTGACA ACTCCTTTAC CAACGTTCAT 
AGCACTTCAG CAAAGTTCCC TGATTACAAA TCAACATGGT CCCCAGATCC CATAGGACAC 
AACCCCACTC ATCTCTCCAA CAAGATGTGG AAAAACCATA TTTCCTCAAG GAACACTACA 
CCGCTGCCCC GCCCACCTCC CGGTCTGACC AACCCCAAAC CATCATCTCC CTGGAGCAGC 
ACAGCACCCC GATCAGTCAG GGGGTGGGGG ACACAGGACT CACGGCTTGC CTCGGCCTCT 
ACCTGGAGTG ATGGTGGTTC AGTTCGTCCC AGTTACTGGC TGGTTCTTCA CAATCTCACT 
CCACAGATTG ATGGGTCAAC GTTGAGAACC ATCTGCATGC AGCATGGCCC ACTGCTGACA 
TTCCATCTGA ACCTAACCCA GGGCACTGCC CTGATCCGAT ACAGCACCAA ACAGGAGGCG 
GCCAAGGCCC AAACTGCACT GCACATGTGT GTGTTGGGAA ACACTACCAT CCTGGCTGAG 
TTTGCCACCG ATGATGAAGT TAGCCGCTTT CTGGCACAAG CTCAGCCCCC TACACCTGCA 
GCAACCCCAA GTGCACCCGC CGCAGGTTGG CAGTCGCTGG AGACCGGCCA GACCCAGTCA 
GATCCCGTCG GACCTGCTTT GAATCTTTTT GGTGGGTCCA CAGGGCTCGG GCAATGGAGC 
AGCAGTGCTG GTGGCAGCAG CGGGGCCGAT CTTGCTGGCG CTTCGTTGTG GGGGCCCCCA 
AACTATTCTT CTAGCTTGTG GGGAGTCCCA GCTGTGGAAG ATCCCCATAG GATGGGCAGC 
CCTGCTCCCT TACTACCTGG TGACCTTCTG GGAGGAGGGT CGGATTCAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424388.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280096.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe291428
Clone ID Related Accession (Same CDS sequence)	XM_022424388.1
Accession Version	XM_022424388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCACC TTCCTCTTTC CATCAATCCA TGTCCCACCA TTTTCCATTC CATAACAGAA 
GTCAGCTTCA TGAGAATACT CTTTGCTTCT ACTTTAATCT CTTCCAGAGA GAAGGAGCAA 
GAAAGGGAAG AACAGTTAAT GGAAGACAAG AAAAGGAAGA AAGAGGATAA AAAGAAAAAA 
GAAGCCACTC AGAAGGTCAC GGAACAAAAA ACCAAAGCAC CTGACATTCT GAAGCATGCT 
GTGAACCACA CACTGATTCT GGGATGTCTT AAGTTTGGAC TTCAAGAAGC AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAACG GCCGCCAGCC CAATTGGCAG CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TAAAACGTGG GCAGCCCCCT CCGCCGTCCT GCATGCTCCT TGGGGGCGGA 
GCAGGGCCTC CTCCCTCCAC AGCACCTGGA GCAAACTCAA ACAACGCACA AGTGACAGGA 
ACGCTGCTGC AGAGTGACAG TGGGACTGCA CCAGAGTCAA CCCTTGGAGG TGCTGCTGCT 
TCAAATTATG CAAATTCCAC TTGGGGCCCG GGAGCCTCCT CCAACAACGG CGCCTCCCCC 
AACCCAATTC ACACCTGGGA CAAGGTGATT GTAGACGGGT CTGACGTGGA AGAGTGGCCT 
TGTATTGCCA GCAAAGACAC TGAGTCTTCT TCCGAAAACA CCACCGATAA CAACAGTGCC 
TCGAACCCTG GCTCTGAGAA GAGCACTCTG CCAGGAAGCA CCACTAGTAA CAAAGGAAAA 
GGGAGCCAGT GCCAGTCTGC AAGTTCTGGG AACGAATGTA ATCTTGGGGT CTGGAAATCT 
GACCCTAAGA CTAAATCTGT TCAATCTTCC AACTCTACAA CAGAGAGCAA CAATGGACTA 
GGAAATTGGA GGAATGTGAG TGGTCAGGAT AGAATTGGAC CTGGCTCTGG CTTCAGCAAC 
TTTAACCCAA ATAGCAACCC ATCTGCCTGG CCAGCGCTGG TCCAAGAAGG AAATTCTAGG 
AAAGGGGCAT TGGAAACGGA TACTAGTAAT TCCAGTGCAC AGGTTAGCAC AGTAGGTCAG 
GCATCCAGGG AACAGCAGTC AAAGATGGAA AATGCGGGTG TTAATTTTGT TGTCTCTGGC 
AGAGAACAGG CTCAGATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC CATGGAGAAT AAGGGAATGC CCTTTGGAAT GGGCTTGGGG 
AACGCCTCCA GGAGCACTGA TGCCCCTTCA CAAAGCACTG GAGATCGAAA GACTGGGAGT 
GTTGGATCTT GGGGTGCAGC TAGGGGGCCT TCTGGAACTG ACACAGCCTC TGGACAAAGC 
AATTCTGGAA ACAATGGGAA CAATGGAAAA GATAGAGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAGAAAT CATCTGGGTC AAAAAATGAC TCTTGGGACA ATAACAGGTC TACGGGTGGG 
TCCTGGAACT TTGGCCCTCA GGACTCTAAT GACAACAAAT GGGGTGAAGG GAACAAAATG 
ACATCTGGGG TCTCTCAGGG AGAATGGAAA CAGCCGACTG GGTCTGATGA GTTGAAAATT 
GGAGAATGGA GTGGTCCAAA CCAACCAAAT TCTAGCACTG GAGCATGGGA CAATCAAAAG 
GGCCACCCCC TCCCTGAAAA CCAAGGCAAT GCCCAGGCTC CCTGTTGGGG AAGATCTTCC 
AGCTCCACAG GAAGTGAAGT TGGAGGTCAA AGCACTGGAA GCAACCACAA AGCAGGAAGT 
AGTGACAGTC ATAATTCTGG CCGTCGGTCA TACCGGCCCA CACATCCTGA TTGTCAGGCT 
GTCTTACAGA CTCTTTTGAG CCGAACTGAT TTGGACCCCA GGGTGCTCTC AAACACTGGC 
TGGGGCCAAA CTCAAATTAA GCAGGACACT GTGTGGGATA TTGAAGAGGT GCCAAGGCCT 
GAGGGGAAAT CGGACAAAGG AACTGAGGGG TGGGAGAGCG CTGCCACACA GACCAAGAAC 
TCAGGGGGCT GGGGAGATGC ACCCAGCCAA AGCAATCAAA TGAAGTCTGG ATGGGGGGAG 
CTCTCAGCCT CTACAGAGTG GAAAGACCCC AAGAACACAG GAGGCTGGAA TGACTATAAG 
AACAACAACT CTTCCAACTG GGGAGGCGGA CGACCAGATG AAAAGACATC TTCCTCTTGG 
AATGAGAACT CCAGCAAGGA TCAGGGGTGG GGAGGGGGAC GCCAGCCCAA TCAAGGATGG 
ACTTCTGGGA AGAATGGTTG GGGAGAGGAA GTTGATCAGG CGAAAAACAG CAGTTGGGAA 
AGTTCTGCAA GCAAACCTGT GTCTGGATGG GGAGAAGGAG GGCAGAATGA AATTGGAACT 
TGGGGGAACA GCGGGAATGC AAGCCTAGCT TCCAAAGGTG GGTGGGAAGA TTGCAAAAGG 
TCTCCAGCGT GGACTGAGAC TGGCAGACAG CCCAATTCCT GGCATAAACA GCACCAACAG 
CAGCAGCAGC CACAGCAGCC ACCGCCACCA CAACCAGAGG CTTCTGGTTC ATGGGGAGGC 
CCCCCACCAC CACCTCCAGG CAACGGACGA CCTTCCAATT CCAACTGGAG CAGTGGGCCA 
CAGCCAGTAA CCCCTAAGGA CGAGGAACCC AGTGGTTGGG AAGAGCCATC CCCACAGTCA 
ATTAGTCGGA AAATGGACAT CGATGATGGC ACTTCAGCAT GGGGAGACCC TAACAGCTAT 
AACTACAAGA ATGTGAATCT GTGGGATAAG AATTCCCAAG GGGGCCCAGC ACCTCGAGAA 
CCAAACCTGC CTACCCCGAT GACCAGTAAA TCAGCATCAG TCTGGAGCAA AAGCACACCA 
CCTGCTCCAG ATAATGGTAC TTCAGCTTGG GGTGAGCCAA ACGAAAGCAG TCCTGGGTGG 
GGCGAAATGG ATGACACGGG AGCATCGACC ACGGGCTGGG GGAATGCACC CTCCAACGCT 
CCAAATGCCA TGAAACCTAA TTCCAAATCT ATGCAAGACG GCTGGGGGGA GAGTGACGGT 
CCAGTGACGG GAACTCGCCA TCCCAGCTGG GAGGAGGAGG ATGATGGAGG AGTCTGGAAC 
ACCGCCGGCT CTCAGGGAAG TGCTTCCTCC CACAACTCAG CAAGCTGGGG ACAAGGAGGA 
AAGAAGCAAA TGAAGTGCTC ACTGAAAGGA GGAAACAATG ATTCATGGAT GAATCCTCTT 
GCCAAACAGT TTTCAAATAT GGGATTGCTG AGTCAAACTG AAGATAATCC AGGCAGCAAA 
ATGGATTTGT CTGTAGGGAG CCTTCCAGAT AAAAAATTTG ACGTGGACAA GCGAGCAATG 
AATCTCGGGG ATTTTAATGA TATCATGAGG AAGGATCGAT CTGGGTTCCG TCCACCTAAT 
TCCAAAGACA TGGGAACCAC AGATAGTGGG CCTTATTTCG AGAAGCTGAC TTTGCCTTTC 
TCCAATCAAG ATGGGTGCCT TGGGGATGAG GCTCCCTGCT CTCCCTTCTC CCCTTCTCCC 
AGCTACAAGC TGTCTCCCTC TGGTTCCACA CTACCCAACG TCAGCCTTGG AGCAATCGGC 
ACAGGGCTCA ACCCCCAAAA CTTCGCTGCT AGACAAGGTG GCAATCATGG TTTGTTTGGA 
AATAGCACAG CACAATCGAG AGGTATGCAC ACACCAGTGC AGCCACTAAA TTCTTCTCCT 
AATCTCCGGG CGCAAGTGCC TCCCCAGTTT ATTTCCCCCC AGGTTTCTGC CTCCATGCTC 
AAGCAGTTTC CCAACAGTGG CCTGAATCCA GGTCTTTTCA ATGTGGGGCC CCAGTTATCT 
CCTCAACAGA TTGCCATGCT GAGCCAGCTT CCACAAATTC CCCAGTTTCA GTTGGCATGT 
CAGCTTCTCC TGCAGCAGCA GCAACAGCAG CAGTTGCTAC AGAACCAGAG GAAGCTTTCT 
CAAGCTGTGC GCCAGCAGCA AGAGCAGCAG CTGGCTCGAA TGGTGAGCGC TCTCCAGCAA 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG AGGCAGCCTG GCATGAAGCA CTCACCCTCT 
CATCCTGTTG GGCCCAAGCC ACATCTGGAC AACATGGTAC CCAACGCTCT GAATGTGGGG 
CTCCCAGACC TTCAAACCAA AGGGCCAATA CCTGGATATG GTTCTGGCTT CAGCTCTGGC 
GGCATGGACT ATGGCATGGT TGGTGGGAAG GAGGCTGGAA CAGAGTCTCG CTTTAAACAG 
TGGACCTCCA TGATGGAGGG ACTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACT CGAGGAGGGT CACCGTACAA CCAGTTTGAT 
ATCATCCCGA GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCCA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT ACTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TCCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACACCGCTG 
CCCCGCCCAC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGATCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TTGCCTCGGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCCAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACGTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACCGATGATG AAGTTAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCAGCAACC 
CCAAGTGCAC CCGCCGCAGG TTGGCAGTCG CTGGAGACCG GCCAGACCCA GTCAGATCCC 
GTCGGACCTG CTTTGAATCT TTTTGGTGGG TCCACAGGGC TCGGGCAATG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCT GGCGCTTCGT TGTGGGGGCC CCCAAACTAT 
TCTTCTAGCT TGTGGGGAGT CCCAGCTGTG GAAGATCCCC ATAGGATGGG CAGCCCTGCT 
CCCTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280096.1
CDS	42..5729
Translation	MTHLPLSINPCPTIFHSITEVSFMRILFASTLISSREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKAPDILKHAVNHTLILGCLKFGLQEAVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424388.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424388.1

ORF Insert Sequence:

ATGACCCACC TTCCTCTTTC CATCAATCCA TGTCCCACCA TTTTCCATTC CATAACAGAA 
GTCAGCTTCA TGAGAATACT CTTTGCTTCT ACTTTAATCT CTTCCAGAGA GAAGGAGCAA 
GAAAGGGAAG AACAGTTAAT GGAAGACAAG AAAAGGAAGA AAGAGGATAA AAAGAAAAAA 
GAAGCCACTC AGAAGGTCAC GGAACAAAAA ACCAAAGCAC CTGACATTCT GAAGCATGCT 
GTGAACCACA CACTGATTCT GGGATGTCTT AAGTTTGGAC TTCAAGAAGC AGTGCCCGAA 
GTGACGAAAC CAAGTTTAAG CCAACCAACG GCCGCCAGCC CAATTGGCAG CTCTCCATCG 
CCACCAGTCA ATGGTGGCAA CAATGCCAAA AGGGTGGCAG TGCCGAACGG ACAACCGCCA 
AGCGCCGCCC GCTACATGCC TCGGGAGGTG CCGCCGCGAT TCCGTTGCCA GCAGGACCAC 
AAAGTGTTAC TAAAACGTGG GCAGCCCCCT CCGCCGTCCT GCATGCTCCT TGGGGGCGGA 
GCAGGGCCTC CTCCCTCCAC AGCACCTGGA GCAAACTCAA ACAACGCACA AGTGACAGGA 
ACGCTGCTGC AGAGTGACAG TGGGACTGCA CCAGAGTCAA CCCTTGGAGG TGCTGCTGCT 
TCAAATTATG CAAATTCCAC TTGGGGCCCG GGAGCCTCCT CCAACAACGG CGCCTCCCCC 
AACCCAATTC ACACCTGGGA CAAGGTGATT GTAGACGGGT CTGACGTGGA AGAGTGGCCT 
TGTATTGCCA GCAAAGACAC TGAGTCTTCT TCCGAAAACA CCACCGATAA CAACAGTGCC 
TCGAACCCTG GCTCTGAGAA GAGCACTCTG CCAGGAAGCA CCACTAGTAA CAAAGGAAAA 
GGGAGCCAGT GCCAGTCTGC AAGTTCTGGG AACGAATGTA ATCTTGGGGT CTGGAAATCT 
GACCCTAAGA CTAAATCTGT TCAATCTTCC AACTCTACAA CAGAGAGCAA CAATGGACTA 
GGAAATTGGA GGAATGTGAG TGGTCAGGAT AGAATTGGAC CTGGCTCTGG CTTCAGCAAC 
TTTAACCCAA ATAGCAACCC ATCTGCCTGG CCAGCGCTGG TCCAAGAAGG AAATTCTAGG 
AAAGGGGCAT TGGAAACGGA TACTAGTAAT TCCAGTGCAC AGGTTAGCAC AGTAGGTCAG 
GCATCCAGGG AACAGCAGTC AAAGATGGAA AATGCGGGTG TTAATTTTGT TGTCTCTGGC 
AGAGAACAGG CTCAGATTCA TAACACTGAT GGACCAAAAA ATGGAAACAC TAACTCCTTG 
AACTTAAGTT CACCAAACCC CATGGAGAAT AAGGGAATGC CCTTTGGAAT GGGCTTGGGG 
AACGCCTCCA GGAGCACTGA TGCCCCTTCA CAAAGCACTG GAGATCGAAA GACTGGGAGT 
GTTGGATCTT GGGGTGCAGC TAGGGGGCCT TCTGGAACTG ACACAGCCTC TGGACAAAGC 
AATTCTGGAA ACAATGGGAA CAATGGAAAA GATAGAGAGG ACTCCTGGAA AGGAGCTTCT 
GTTCAGAAAT CATCTGGGTC AAAAAATGAC TCTTGGGACA ATAACAGGTC TACGGGTGGG 
TCCTGGAACT TTGGCCCTCA GGACTCTAAT GACAACAAAT GGGGTGAAGG GAACAAAATG 
ACATCTGGGG TCTCTCAGGG AGAATGGAAA CAGCCGACTG GGTCTGATGA GTTGAAAATT 
GGAGAATGGA GTGGTCCAAA CCAACCAAAT TCTAGCACTG GAGCATGGGA CAATCAAAAG 
GGCCACCCCC TCCCTGAAAA CCAAGGCAAT GCCCAGGCTC CCTGTTGGGG AAGATCTTCC 
AGCTCCACAG GAAGTGAAGT TGGAGGTCAA AGCACTGGAA GCAACCACAA AGCAGGAAGT 
AGTGACAGTC ATAATTCTGG CCGTCGGTCA TACCGGCCCA CACATCCTGA TTGTCAGGCT 
GTCTTACAGA CTCTTTTGAG CCGAACTGAT TTGGACCCCA GGGTGCTCTC AAACACTGGC 
TGGGGCCAAA CTCAAATTAA GCAGGACACT GTGTGGGATA TTGAAGAGGT GCCAAGGCCT 
GAGGGGAAAT CGGACAAAGG AACTGAGGGG TGGGAGAGCG CTGCCACACA GACCAAGAAC 
TCAGGGGGCT GGGGAGATGC ACCCAGCCAA AGCAATCAAA TGAAGTCTGG ATGGGGGGAG 
CTCTCAGCCT CTACAGAGTG GAAAGACCCC AAGAACACAG GAGGCTGGAA TGACTATAAG 
AACAACAACT CTTCCAACTG GGGAGGCGGA CGACCAGATG AAAAGACATC TTCCTCTTGG 
AATGAGAACT CCAGCAAGGA TCAGGGGTGG GGAGGGGGAC GCCAGCCCAA TCAAGGATGG 
ACTTCTGGGA AGAATGGTTG GGGAGAGGAA GTTGATCAGG CGAAAAACAG CAGTTGGGAA 
AGTTCTGCAA GCAAACCTGT GTCTGGATGG GGAGAAGGAG GGCAGAATGA AATTGGAACT 
TGGGGGAACA GCGGGAATGC AAGCCTAGCT TCCAAAGGTG GGTGGGAAGA TTGCAAAAGG 
TCTCCAGCGT GGACTGAGAC TGGCAGACAG CCCAATTCCT GGCATAAACA GCACCAACAG 
CAGCAGCAGC CACAGCAGCC ACCGCCACCA CAACCAGAGG CTTCTGGTTC ATGGGGAGGC 
CCCCCACCAC CACCTCCAGG CAACGGACGA CCTTCCAATT CCAACTGGAG CAGTGGGCCA 
CAGCCAGTAA CCCCTAAGGA CGAGGAACCC AGTGGTTGGG AAGAGCCATC CCCACAGTCA 
ATTAGTCGGA AAATGGACAT CGATGATGGC ACTTCAGCAT GGGGAGACCC TAACAGCTAT 
AACTACAAGA ATGTGAATCT GTGGGATAAG AATTCCCAAG GGGGCCCAGC ACCTCGAGAA 
CCAAACCTGC CTACCCCGAT GACCAGTAAA TCAGCATCAG TCTGGAGCAA AAGCACACCA 
CCTGCTCCAG ATAATGGTAC TTCAGCTTGG GGTGAGCCAA ACGAAAGCAG TCCTGGGTGG 
GGCGAAATGG ATGACACGGG AGCATCGACC ACGGGCTGGG GGAATGCACC CTCCAACGCT 
CCAAATGCCA TGAAACCTAA TTCCAAATCT ATGCAAGACG GCTGGGGGGA GAGTGACGGT 
CCAGTGACGG GAACTCGCCA TCCCAGCTGG GAGGAGGAGG ATGATGGAGG AGTCTGGAAC 
ACCGCCGGCT CTCAGGGAAG TGCTTCCTCC CACAACTCAG CAAGCTGGGG ACAAGGAGGA 
AAGAAGCAAA TGAAGTGCTC ACTGAAAGGA GGAAACAATG ATTCATGGAT GAATCCTCTT 
GCCAAACAGT TTTCAAATAT GGGATTGCTG AGTCAAACTG AAGATAATCC AGGCAGCAAA 
ATGGATTTGT CTGTAGGGAG CCTTCCAGAT AAAAAATTTG ACGTGGACAA GCGAGCAATG 
AATCTCGGGG ATTTTAATGA TATCATGAGG AAGGATCGAT CTGGGTTCCG TCCACCTAAT 
TCCAAAGACA TGGGAACCAC AGATAGTGGG CCTTATTTCG AGAAGCTGAC TTTGCCTTTC 
TCCAATCAAG ATGGGTGCCT TGGGGATGAG GCTCCCTGCT CTCCCTTCTC CCCTTCTCCC 
AGCTACAAGC TGTCTCCCTC TGGTTCCACA CTACCCAACG TCAGCCTTGG AGCAATCGGC 
ACAGGGCTCA ACCCCCAAAA CTTCGCTGCT AGACAAGGTG GCAATCATGG TTTGTTTGGA 
AATAGCACAG CACAATCGAG AGGTATGCAC ACACCAGTGC AGCCACTAAA TTCTTCTCCT 
AATCTCCGGG CGCAAGTGCC TCCCCAGTTT ATTTCCCCCC AGGTTTCTGC CTCCATGCTC 
AAGCAGTTTC CCAACAGTGG CCTGAATCCA GGTCTTTTCA ATGTGGGGCC CCAGTTATCT 
CCTCAACAGA TTGCCATGCT GAGCCAGCTT CCACAAATTC CCCAGTTTCA GTTGGCATGT 
CAGCTTCTCC TGCAGCAGCA GCAACAGCAG CAGTTGCTAC AGAACCAGAG GAAGCTTTCT 
CAAGCTGTGC GCCAGCAGCA AGAGCAGCAG CTGGCTCGAA TGGTGAGCGC TCTCCAGCAA 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG AGGCAGCCTG GCATGAAGCA CTCACCCTCT 
CATCCTGTTG GGCCCAAGCC ACATCTGGAC AACATGGTAC CCAACGCTCT GAATGTGGGG 
CTCCCAGACC TTCAAACCAA AGGGCCAATA CCTGGATATG GTTCTGGCTT CAGCTCTGGC 
GGCATGGACT ATGGCATGGT TGGTGGGAAG GAGGCTGGAA CAGAGTCTCG CTTTAAACAG 
TGGACCTCCA TGATGGAGGG ACTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACT CGAGGAGGGT CACCGTACAA CCAGTTTGAT 
ATCATCCCGA GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCCA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT ACTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TCCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACACCGCTG 
CCCCGCCCAC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGATCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TTGCCTCGGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCCAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACGTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACCGATGATG AAGTTAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCAGCAACC 
CCAAGTGCAC CCGCCGCAGG TTGGCAGTCG CTGGAGACCG GCCAGACCCA GTCAGATCCC 
GTCGGACCTG CTTTGAATCT TTTTGGTGGG TCCACAGGGC TCGGGCAATG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCT GGCGCTTCGT TGTGGGGGCC CCCAAACTAT 
TCTTCTAGCT TGTGGGGAGT CCCAGCTGTG GAAGATCCCC ATAGGATGGG CAGCCCTGCT 
CCCTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424389.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280097.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe291429
Clone ID Related Accession (Same CDS sequence)	XM_022424389.1
Accession Version	XM_022424389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC ATCAGGATTG CTTCTGGCTG TATGACCCAC CTTCCTCTTT CCATCAATCC 
ATGTCCCACC ATTTTCCATT CCATAACAGA AGTCAGCTTC ATGAGAATAC TCTTTGCTTC 
TACTTTAATC TCTTCCAGGG TGCAGTGATC CAGAATCCAG AAGGGCTGTT AGTTGAGAGA 
GAGAAGGAGC AAGAAAGGGA AGAACAGTTA ATGGAAGACA AGAAAAGGAA GAAAGAGGAT 
AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC ACGGAACAAA AAACCAAAGT GCCCGAAGTG 
ACGAAACCAA GTTTAAGCCA ACCAACGGCC GCCAGCCCAA TTGGCAGCTC TCCATCGCCA 
CCAGTCAATG GTGGCAACAA TGCCAAAAGG GTGGCAGTGC CGAACGGACA ACCGCCAAGC 
GCCGCCCGCT ACATGCCTCG GGAGGTGCCG CCGCGATTCC GTTGCCAGCA GGACCACAAA 
GTGTTACTAA AACGTGGGCA GCCCCCTCCG CCGTCCTGCA TGCTCCTTGG GGGCGGAGCA 
GGGCCTCCTC CCTCCACAGC ACCTGGAGCA AACTCAAACA ACGCACAAGT GACAGGAACG 
CTGCTGCAGA GTGACAGTGG GACTGCACCA GAGTCAACCC TTGGAGGTGC TGCTGCTTCA 
AATTATGCAA ATTCCACTTG GGGCCCGGGA GCCTCCTCCA ACAACGGCGC CTCCCCCAAC 
CCAATTCACA CCTGGGACAA GGTGATTGTA GACGGGTCTG ACGTGGAAGA GTGGCCTTGT 
ATTGCCAGCA AAGACACTGA GTCTTCTTCC GAAAACACCA CCGATAACAA CAGTGCCTCG 
AACCCTGGCT CTGAGAAGAG CACTCTGCCA GGAAGCACCA CTAGTAACAA AGGAAAAGGG 
AGCCAGTGCC AGTCTGCAAG TTCTGGGAAC GAATGTAATC TTGGGGTCTG GAAATCTGAC 
CCTAAGACTA AATCTGTTCA ATCTTCCAAC TCTACAACAG AGAGCAACAA TGGACTAGGA 
AATTGGAGGA ATGTGAGTGG TCAGGATAGA ATTGGACCTG GCTCTGGCTT CAGCAACTTT 
AACCCAAATA GCAACCCATC TGCCTGGCCA GCGCTGGTCC AAGAAGGAAA TTCTAGGAAA 
GGGGCATTGG AAACGGATAC TAGTAATTCC AGTGCACAGG TTAGCACAGT AGGTCAGGCA 
TCCAGGGAAC AGCAGTCAAA GATGGAAAAT GCGGGTGTTA ATTTTGTTGT CTCTGGCAGA 
GAACAGGCTC AGATTCATAA CACTGATGGA CCAAAAAATG GAAACACTAA CTCCTTGAAC 
TTAAGTTCAC CAAACCCCAT GGAGAATAAG GGAATGCCCT TTGGAATGGG CTTGGGGAAC 
GCCTCCAGGA GCACTGATGC CCCTTCACAA AGCACTGGAG ATCGAAAGAC TGGGAGTGTT 
GGATCTTGGG GTGCAGCTAG GGGGCCTTCT GGAACTGACA CAGCCTCTGG ACAAAGCAAT 
TCTGGAAACA ATGGGAACAA TGGAAAAGAT AGAGAGGACT CCTGGAAAGG AGCTTCTGTT 
CAGAAATCAT CTGGGTCAAA AAATGACTCT TGGGACAATA ACAGGTCTAC GGGTGGGTCC 
TGGAACTTTG GCCCTCAGGA CTCTAATGAC AACAAATGGG GTGAAGGGAA CAAAATGACA 
TCTGGGGTCT CTCAGGGAGA ATGGAAACAG CCGACTGGGT CTGATGAGTT GAAAATTGGA 
GAATGGAGTG GTCCAAACCA ACCAAATTCT AGCACTGGAG CATGGGACAA TCAAAAGGGC 
CACCCCCTCC CTGAAAACCA AGGCAATGCC CAGGCTCCCT GTTGGGGAAG ATCTTCCAGC 
TCCACAGGAA GTGAAGTTGG AGGTCAAAGC ACTGGAAGCA ACCACAAAGC AGGAAGTAGT 
GACAGTCATA ATTCTGGCCG TCGGTCATAC CGGCCCACAC ATCCTGATTG TCAGGCTGTC 
TTACAGACTC TTTTGAGCCG AACTGATTTG GACCCCAGGG TGCTCTCAAA CACTGGCTGG 
GGCCAAACTC AAATTAAGCA GGACACTGTG TGGGATATTG AAGAGGTGCC AAGGCCTGAG 
GGGAAATCGG ACAAAGGAAC TGAGGGGTGG GAGAGCGCTG CCACACAGAC CAAGAACTCA 
GGGGGCTGGG GAGATGCACC CAGCCAAAGC AATCAAATGA AGTCTGGATG GGGGGAGCTC 
TCAGCCTCTA CAGAGTGGAA AGACCCCAAG AACACAGGAG GCTGGAATGA CTATAAGAAC 
AACAACTCTT CCAACTGGGG AGGCGGACGA CCAGATGAAA AGACATCTTC CTCTTGGAAT 
GAGAACTCCA GCAAGGATCA GGGGTGGGGA GGGGGACGCC AGCCCAATCA AGGATGGACT 
TCTGGGAAGA ATGGTTGGGG AGAGGAAGTT GATCAGGCGA AAAACAGCAG TTGGGAAAGT 
TCTGCAAGCA AACCTGTGTC TGGATGGGGA GAAGGAGGGC AGAATGAAAT TGGAACTTGG 
GGGAACAGCG GGAATGCAAG CCTAGCTTCC AAAGGTGGGT GGGAAGATTG CAAAAGGTCT 
CCAGCGTGGA CTGAGACTGG CAGACAGCCC AATTCCTGGC ATAAACAGCA CCAACAGCAG 
CAGCAGCCAC AGCAGCCACC GCCACCACAA CCAGAGGCTT CTGGTTCATG GGGAGGCCCC 
CCACCACCAC CTCCAGGCAA CGGACGACCT TCCAATTCCA ACTGGAGCAG TGGGCCACAG 
CCAGTAACCC CTAAGGACGA GGAACCCAGT GGTTGGGAAG AGCCATCCCC ACAGTCAATT 
AGTCGGAAAA TGGACATCGA TGATGGCACT TCAGCATGGG GAGACCCTAA CAGCTATAAC 
TACAAGAATG TGAATCTGTG GGATAAGAAT TCCCAAGGGG GCCCAGCACC TCGAGAACCA 
AACCTGCCTA CCCCGATGAC CAGTAAATCA GCATCAGTCT GGAGCAAAAG CACACCACCT 
GCTCCAGATA ATGGTACTTC AGCTTGGGGT GAGCCAAACG AAAGCAGTCC TGGGTGGGGC 
GAAATGGATG ACACGGGAGC ATCGACCACG GGCTGGGGGA ATGCACCCTC CAACGCTCCA 
AATGCCATGA AACCTAATTC CAAATCTATG CAAGACGGCT GGGGGGAGAG TGACGGTCCA 
GTGACGGGAA CTCGCCATCC CAGCTGGGAG GAGGAGGATG ATGGAGGAGT CTGGAACACC 
GCCGGCTCTC AGGGAAGTGC TTCCTCCCAC AACTCAGCAA GCTGGGGACA AGGAGGAAAG 
AAGCAAATGA AGTGCTCACT GAAAGGAGGA AACAATGATT CATGGATGAA TCCTCTTGCC 
AAACAGTTTT CAAATATGGG ATTGCTGAGT CAAACTGAAG ATAATCCAGG CAGCAAAATG 
GATTTGTCTG TAGGGAGCCT TCCAGATAAA AAATTTGACG TGGACAAGCG AGCAATGAAT 
CTCGGGGATT TTAATGATAT CATGAGGAAG GATCGATCTG GGTTCCGTCC ACCTAATTCC 
AAAGACATGG GAACCACAGA TAGTGGGCCT TATTTCGAGA AGCTGACTTT GCCTTTCTCC 
AATCAAGATG GGTGCCTTGG GGATGAGGCT CCCTGCTCTC CCTTCTCCCC TTCTCCCAGC 
TACAAGCTGT CTCCCTCTGG TTCCACACTA CCCAACGTCA GCCTTGGAGC AATCGGCACA 
GGGCTCAACC CCCAAAACTT CGCTGCTAGA CAAGGTGGCA ATCATGGTTT GTTTGGAAAT 
AGCACAGCAC AATCGAGAGG TATGCACACA CCAGTGCAGC CACTAAATTC TTCTCCTAAT 
CTCCGGGCGC AAGTGCCTCC CCAGTTTATT TCCCCCCAGG TTTCTGCCTC CATGCTCAAG 
CAGTTTCCCA ACAGTGGCCT GAATCCAGGT CTTTTCAATG TGGGGCCCCA GTTATCTCCT 
CAACAGATTG CCATGCTGAG CCAGCTTCCA CAAATTCCCC AGTTTCAGTT GGCATGTCAG 
CTTCTCCTGC AGCAGCAGCA ACAGCAGCAG TTGCTACAGA ACCAGAGGAA GCTTTCTCAA 
GCTGTGCGCC AGCAGCAAGA GCAGCAGCTG GCTCGAATGG TGAGCGCTCT CCAGCAACAG 
CAGCAACAGC AGCAGCAGCA GCAGCAGAGG CAGCCTGGCA TGAAGCACTC ACCCTCTCAT 
CCTGTTGGGC CCAAGCCACA TCTGGACAAC ATGGTACCCA ACGCTCTGAA TGTGGGGCTC 
CCAGACCTTC AAACCAAAGG GCCAATACCT GGATATGGTT CTGGCTTCAG CTCTGGCGGC 
ATGGACTATG GCATGGTTGG TGGGAAGGAG GCTGGAACAG AGTCTCGCTT TAAACAGTGG 
ACCTCCATGA TGGAGGGACT GCCCTCTGTA GCCACACAGG AAGCCAATAT GCACAAAAAT 
GGCGCTATTG TGGCCCCTGG TAAGACTCGA GGAGGGTCAC CGTACAACCA GTTTGATATC 
ATCCCGAGTG ACACACTGGG TGGCCATACG GGTCCTGCTG GTGATAGCTG GTTACCTGCC 
AAATCTCCAC CAACAAATAA AATCGGAAGT AAATCCAGCA ATGCCAGTTG GCCTCCAGAA 
TTCCAACCAG GAGTGCCATG GAAAGGTATC CAAAACATTG ACCCTGAATC TGACCCCTAT 
GTCACCCCAG GAAGTGTACT GGGGGGTACA GCCACATCTC CCATTGTAGA TACTGACCAC 
CAACTTCTGC GGGATAACAC CACAGGGTCT AATTCTTCCC TCAACACCTC GCTGCCTTCA 
CCTGGTGCCT GGCCCTACAG TGCCTCTGAC AACTCCTTTA CCAACGTTCA TAGCACTTCA 
GCAAAGTTCC CTGATTACAA ATCAACATGG TCCCCAGATC CCATAGGACA CAACCCCACT 
CATCTCTCCA ACAAGATGTG GAAAAACCAT ATTTCCTCAA GGAACACTAC ACCGCTGCCC 
CGCCCACCTC CCGGTCTGAC CAACCCCAAA CCATCATCTC CCTGGAGCAG CACAGCACCC 
CGATCAGTCA GGGGGTGGGG GACACAGGAC TCACGGCTTG CCTCGGCCTC TACCTGGAGT 
GATGGTGGTT CAGTTCGTCC CAGTTACTGG CTGGTTCTTC ACAATCTCAC TCCACAGATT 
GATGGGTCAA CGTTGAGAAC CATCTGCATG CAGCATGGCC CACTGCTGAC ATTCCATCTG 
AACCTAACCC AGGGCACTGC CCTGATCCGA TACAGCACCA AACAGGAGGC GGCCAAGGCC 
CAAACTGCAC TGCACATGTG TGTGTTGGGA AACACTACCA TCCTGGCTGA GTTTGCCACC 
GATGATGAAG TTAGCCGCTT TCTGGCACAA GCTCAGCCCC CTACACCTGC AGCAACCCCA 
AGTGCACCCG CCGCAGGTTG GCAGTCGCTG GAGACCGGCC AGACCCAGTC AGATCCCGTC 
GGACCTGCTT TGAATCTTTT TGGTGGGTCC ACAGGGCTCG GGCAATGGAG CAGCAGTGCT 
GGTGGCAGCA GCGGGGCCGA TCTTGCTGGC GCTTCGTTGT GGGGGCCCCC AAACTATTCT 
TCTAGCTTGT GGGGAGTCCC AGCTGTGGAA GATCCCCATA GGATGGGCAG CCCTGCTCCC 
TTACTACCTG GTGACCTTCT GGGAGGAGGG TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280097.1
CDS	16..5700
Translation	MDSHQDCFWLYDPPSSFHQSMSHHFPFHNRSQLHENTLCFYFNLFQGAVIQNPEGLLVEREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424389.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424389.1

ORF Insert Sequence:

ATGGACTCCC ATCAGGATTG CTTCTGGCTG TATGACCCAC CTTCCTCTTT CCATCAATCC 
ATGTCCCACC ATTTTCCATT CCATAACAGA AGTCAGCTTC ATGAGAATAC TCTTTGCTTC 
TACTTTAATC TCTTCCAGGG TGCAGTGATC CAGAATCCAG AAGGGCTGTT AGTTGAGAGA 
GAGAAGGAGC AAGAAAGGGA AGAACAGTTA ATGGAAGACA AGAAAAGGAA GAAAGAGGAT 
AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC ACGGAACAAA AAACCAAAGT GCCCGAAGTG 
ACGAAACCAA GTTTAAGCCA ACCAACGGCC GCCAGCCCAA TTGGCAGCTC TCCATCGCCA 
CCAGTCAATG GTGGCAACAA TGCCAAAAGG GTGGCAGTGC CGAACGGACA ACCGCCAAGC 
GCCGCCCGCT ACATGCCTCG GGAGGTGCCG CCGCGATTCC GTTGCCAGCA GGACCACAAA 
GTGTTACTAA AACGTGGGCA GCCCCCTCCG CCGTCCTGCA TGCTCCTTGG GGGCGGAGCA 
GGGCCTCCTC CCTCCACAGC ACCTGGAGCA AACTCAAACA ACGCACAAGT GACAGGAACG 
CTGCTGCAGA GTGACAGTGG GACTGCACCA GAGTCAACCC TTGGAGGTGC TGCTGCTTCA 
AATTATGCAA ATTCCACTTG GGGCCCGGGA GCCTCCTCCA ACAACGGCGC CTCCCCCAAC 
CCAATTCACA CCTGGGACAA GGTGATTGTA GACGGGTCTG ACGTGGAAGA GTGGCCTTGT 
ATTGCCAGCA AAGACACTGA GTCTTCTTCC GAAAACACCA CCGATAACAA CAGTGCCTCG 
AACCCTGGCT CTGAGAAGAG CACTCTGCCA GGAAGCACCA CTAGTAACAA AGGAAAAGGG 
AGCCAGTGCC AGTCTGCAAG TTCTGGGAAC GAATGTAATC TTGGGGTCTG GAAATCTGAC 
CCTAAGACTA AATCTGTTCA ATCTTCCAAC TCTACAACAG AGAGCAACAA TGGACTAGGA 
AATTGGAGGA ATGTGAGTGG TCAGGATAGA ATTGGACCTG GCTCTGGCTT CAGCAACTTT 
AACCCAAATA GCAACCCATC TGCCTGGCCA GCGCTGGTCC AAGAAGGAAA TTCTAGGAAA 
GGGGCATTGG AAACGGATAC TAGTAATTCC AGTGCACAGG TTAGCACAGT AGGTCAGGCA 
TCCAGGGAAC AGCAGTCAAA GATGGAAAAT GCGGGTGTTA ATTTTGTTGT CTCTGGCAGA 
GAACAGGCTC AGATTCATAA CACTGATGGA CCAAAAAATG GAAACACTAA CTCCTTGAAC 
TTAAGTTCAC CAAACCCCAT GGAGAATAAG GGAATGCCCT TTGGAATGGG CTTGGGGAAC 
GCCTCCAGGA GCACTGATGC CCCTTCACAA AGCACTGGAG ATCGAAAGAC TGGGAGTGTT 
GGATCTTGGG GTGCAGCTAG GGGGCCTTCT GGAACTGACA CAGCCTCTGG ACAAAGCAAT 
TCTGGAAACA ATGGGAACAA TGGAAAAGAT AGAGAGGACT CCTGGAAAGG AGCTTCTGTT 
CAGAAATCAT CTGGGTCAAA AAATGACTCT TGGGACAATA ACAGGTCTAC GGGTGGGTCC 
TGGAACTTTG GCCCTCAGGA CTCTAATGAC AACAAATGGG GTGAAGGGAA CAAAATGACA 
TCTGGGGTCT CTCAGGGAGA ATGGAAACAG CCGACTGGGT CTGATGAGTT GAAAATTGGA 
GAATGGAGTG GTCCAAACCA ACCAAATTCT AGCACTGGAG CATGGGACAA TCAAAAGGGC 
CACCCCCTCC CTGAAAACCA AGGCAATGCC CAGGCTCCCT GTTGGGGAAG ATCTTCCAGC 
TCCACAGGAA GTGAAGTTGG AGGTCAAAGC ACTGGAAGCA ACCACAAAGC AGGAAGTAGT 
GACAGTCATA ATTCTGGCCG TCGGTCATAC CGGCCCACAC ATCCTGATTG TCAGGCTGTC 
TTACAGACTC TTTTGAGCCG AACTGATTTG GACCCCAGGG TGCTCTCAAA CACTGGCTGG 
GGCCAAACTC AAATTAAGCA GGACACTGTG TGGGATATTG AAGAGGTGCC AAGGCCTGAG 
GGGAAATCGG ACAAAGGAAC TGAGGGGTGG GAGAGCGCTG CCACACAGAC CAAGAACTCA 
GGGGGCTGGG GAGATGCACC CAGCCAAAGC AATCAAATGA AGTCTGGATG GGGGGAGCTC 
TCAGCCTCTA CAGAGTGGAA AGACCCCAAG AACACAGGAG GCTGGAATGA CTATAAGAAC 
AACAACTCTT CCAACTGGGG AGGCGGACGA CCAGATGAAA AGACATCTTC CTCTTGGAAT 
GAGAACTCCA GCAAGGATCA GGGGTGGGGA GGGGGACGCC AGCCCAATCA AGGATGGACT 
TCTGGGAAGA ATGGTTGGGG AGAGGAAGTT GATCAGGCGA AAAACAGCAG TTGGGAAAGT 
TCTGCAAGCA AACCTGTGTC TGGATGGGGA GAAGGAGGGC AGAATGAAAT TGGAACTTGG 
GGGAACAGCG GGAATGCAAG CCTAGCTTCC AAAGGTGGGT GGGAAGATTG CAAAAGGTCT 
CCAGCGTGGA CTGAGACTGG CAGACAGCCC AATTCCTGGC ATAAACAGCA CCAACAGCAG 
CAGCAGCCAC AGCAGCCACC GCCACCACAA CCAGAGGCTT CTGGTTCATG GGGAGGCCCC 
CCACCACCAC CTCCAGGCAA CGGACGACCT TCCAATTCCA ACTGGAGCAG TGGGCCACAG 
CCAGTAACCC CTAAGGACGA GGAACCCAGT GGTTGGGAAG AGCCATCCCC ACAGTCAATT 
AGTCGGAAAA TGGACATCGA TGATGGCACT TCAGCATGGG GAGACCCTAA CAGCTATAAC 
TACAAGAATG TGAATCTGTG GGATAAGAAT TCCCAAGGGG GCCCAGCACC TCGAGAACCA 
AACCTGCCTA CCCCGATGAC CAGTAAATCA GCATCAGTCT GGAGCAAAAG CACACCACCT 
GCTCCAGATA ATGGTACTTC AGCTTGGGGT GAGCCAAACG AAAGCAGTCC TGGGTGGGGC 
GAAATGGATG ACACGGGAGC ATCGACCACG GGCTGGGGGA ATGCACCCTC CAACGCTCCA 
AATGCCATGA AACCTAATTC CAAATCTATG CAAGACGGCT GGGGGGAGAG TGACGGTCCA 
GTGACGGGAA CTCGCCATCC CAGCTGGGAG GAGGAGGATG ATGGAGGAGT CTGGAACACC 
GCCGGCTCTC AGGGAAGTGC TTCCTCCCAC AACTCAGCAA GCTGGGGACA AGGAGGAAAG 
AAGCAAATGA AGTGCTCACT GAAAGGAGGA AACAATGATT CATGGATGAA TCCTCTTGCC 
AAACAGTTTT CAAATATGGG ATTGCTGAGT CAAACTGAAG ATAATCCAGG CAGCAAAATG 
GATTTGTCTG TAGGGAGCCT TCCAGATAAA AAATTTGACG TGGACAAGCG AGCAATGAAT 
CTCGGGGATT TTAATGATAT CATGAGGAAG GATCGATCTG GGTTCCGTCC ACCTAATTCC 
AAAGACATGG GAACCACAGA TAGTGGGCCT TATTTCGAGA AGCTGACTTT GCCTTTCTCC 
AATCAAGATG GGTGCCTTGG GGATGAGGCT CCCTGCTCTC CCTTCTCCCC TTCTCCCAGC 
TACAAGCTGT CTCCCTCTGG TTCCACACTA CCCAACGTCA GCCTTGGAGC AATCGGCACA 
GGGCTCAACC CCCAAAACTT CGCTGCTAGA CAAGGTGGCA ATCATGGTTT GTTTGGAAAT 
AGCACAGCAC AATCGAGAGG TATGCACACA CCAGTGCAGC CACTAAATTC TTCTCCTAAT 
CTCCGGGCGC AAGTGCCTCC CCAGTTTATT TCCCCCCAGG TTTCTGCCTC CATGCTCAAG 
CAGTTTCCCA ACAGTGGCCT GAATCCAGGT CTTTTCAATG TGGGGCCCCA GTTATCTCCT 
CAACAGATTG CCATGCTGAG CCAGCTTCCA CAAATTCCCC AGTTTCAGTT GGCATGTCAG 
CTTCTCCTGC AGCAGCAGCA ACAGCAGCAG TTGCTACAGA ACCAGAGGAA GCTTTCTCAA 
GCTGTGCGCC AGCAGCAAGA GCAGCAGCTG GCTCGAATGG TGAGCGCTCT CCAGCAACAG 
CAGCAACAGC AGCAGCAGCA GCAGCAGAGG CAGCCTGGCA TGAAGCACTC ACCCTCTCAT 
CCTGTTGGGC CCAAGCCACA TCTGGACAAC ATGGTACCCA ACGCTCTGAA TGTGGGGCTC 
CCAGACCTTC AAACCAAAGG GCCAATACCT GGATATGGTT CTGGCTTCAG CTCTGGCGGC 
ATGGACTATG GCATGGTTGG TGGGAAGGAG GCTGGAACAG AGTCTCGCTT TAAACAGTGG 
ACCTCCATGA TGGAGGGACT GCCCTCTGTA GCCACACAGG AAGCCAATAT GCACAAAAAT 
GGCGCTATTG TGGCCCCTGG TAAGACTCGA GGAGGGTCAC CGTACAACCA GTTTGATATC 
ATCCCGAGTG ACACACTGGG TGGCCATACG GGTCCTGCTG GTGATAGCTG GTTACCTGCC 
AAATCTCCAC CAACAAATAA AATCGGAAGT AAATCCAGCA ATGCCAGTTG GCCTCCAGAA 
TTCCAACCAG GAGTGCCATG GAAAGGTATC CAAAACATTG ACCCTGAATC TGACCCCTAT 
GTCACCCCAG GAAGTGTACT GGGGGGTACA GCCACATCTC CCATTGTAGA TACTGACCAC 
CAACTTCTGC GGGATAACAC CACAGGGTCT AATTCTTCCC TCAACACCTC GCTGCCTTCA 
CCTGGTGCCT GGCCCTACAG TGCCTCTGAC AACTCCTTTA CCAACGTTCA TAGCACTTCA 
GCAAAGTTCC CTGATTACAA ATCAACATGG TCCCCAGATC CCATAGGACA CAACCCCACT 
CATCTCTCCA ACAAGATGTG GAAAAACCAT ATTTCCTCAA GGAACACTAC ACCGCTGCCC 
CGCCCACCTC CCGGTCTGAC CAACCCCAAA CCATCATCTC CCTGGAGCAG CACAGCACCC 
CGATCAGTCA GGGGGTGGGG GACACAGGAC TCACGGCTTG CCTCGGCCTC TACCTGGAGT 
GATGGTGGTT CAGTTCGTCC CAGTTACTGG CTGGTTCTTC ACAATCTCAC TCCACAGATT 
GATGGGTCAA CGTTGAGAAC CATCTGCATG CAGCATGGCC CACTGCTGAC ATTCCATCTG 
AACCTAACCC AGGGCACTGC CCTGATCCGA TACAGCACCA AACAGGAGGC GGCCAAGGCC 
CAAACTGCAC TGCACATGTG TGTGTTGGGA AACACTACCA TCCTGGCTGA GTTTGCCACC 
GATGATGAAG TTAGCCGCTT TCTGGCACAA GCTCAGCCCC CTACACCTGC AGCAACCCCA 
AGTGCACCCG CCGCAGGTTG GCAGTCGCTG GAGACCGGCC AGACCCAGTC AGATCCCGTC 
GGACCTGCTT TGAATCTTTT TGGTGGGTCC ACAGGGCTCG GGCAATGGAG CAGCAGTGCT 
GGTGGCAGCA GCGGGGCCGA TCTTGCTGGC GCTTCGTTGT GGGGGCCCCC AAACTATTCT 
TCTAGCTTGT GGGGAGTCCC AGCTGTGGAA GATCCCCATA GGATGGGCAG CCCTGCTCCC 
TTACTACCTG GTGACCTTCT GGGAGGAGGG TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424390.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280098.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

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CloneID	OCe291430
Clone ID Related Accession (Same CDS sequence)	XM_022424390.1
Accession Version	XM_022424390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAA TGCTGGCCCC TATCTTTCTG CAGGGTGCAG TGATCCAGAA TCCAGAAGGG 
CTGTTAGTTG AGAGAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAAAAAGAA GCCACTCAGA AGGTCACGGA ACAAAAAACC 
AAAGCACCTG ACATTCTGAA GCATGCTGTG AACCACACAC TGATTCTGGG ATGTCTTAAG 
TTTGGACTTC AAGAAGCAGT GCCCGAAGTG ACGAAACCAA GTTTAAGCCA ACCAACGGCC 
GCCAGCCCAA TTGGCAGCTC TCCATCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCAAGC GCCGCCCGCT ACATGCCTCG GGAGGTGCCG 
CCGCGATTCC GTTGCCAGCA GGACCACAAA GTGTTACTAA AACGTGGGCA GCCCCCTCCG 
CCGTCCTGCA TGCTCCTTGG GGGCGGAGCA GGGCCTCCTC CCTCCACAGC ACCTGGAGCA 
AACTCAAACA ACGCACAAGT GACAGGAACG CTGCTGCAGA GTGACAGTGG GACTGCACCA 
GAGTCAACCC TTGGAGGTGC TGCTGCTTCA AATTATGCAA ATTCCACTTG GGGCCCGGGA 
GCCTCCTCCA ACAACGGCGC CTCCCCCAAC CCAATTCACA CCTGGGACAA GGTGATTGTA 
GACGGGTCTG ACGTGGAAGA GTGGCCTTGT ATTGCCAGCA AAGACACTGA GTCTTCTTCC 
GAAAACACCA CCGATAACAA CAGTGCCTCG AACCCTGGCT CTGAGAAGAG CACTCTGCCA 
GGAAGCACCA CTAGTAACAA AGGAAAAGGG AGCCAGTGCC AGTCTGCAAG TTCTGGGAAC 
GAATGTAATC TTGGGGTCTG GAAATCTGAC CCTAAGACTA AATCTGTTCA ATCTTCCAAC 
TCTACAACAG AGAGCAACAA TGGACTAGGA AATTGGAGGA ATGTGAGTGG TCAGGATAGA 
ATTGGACCTG GCTCTGGCTT CAGCAACTTT AACCCAAATA GCAACCCATC TGCCTGGCCA 
GCGCTGGTCC AAGAAGGAAA TTCTAGGAAA GGGGCATTGG AAACGGATAC TAGTAATTCC 
AGTGCACAGG TTAGCACAGT AGGTCAGGCA TCCAGGGAAC AGCAGTCAAA GATGGAAAAT 
GCGGGTGTTA ATTTTGTTGT CTCTGGCAGA GAACAGGCTC AGATTCATAA CACTGATGGA 
CCAAAAAATG GAAACACTAA CTCCTTGAAC TTAAGTTCAC CAAACCCCAT GGAGAATAAG 
GGAATGCCCT TTGGAATGGG CTTGGGGAAC GCCTCCAGGA GCACTGATGC CCCTTCACAA 
AGCACTGGAG ATCGAAAGAC TGGGAGTGTT GGATCTTGGG GTGCAGCTAG GGGGCCTTCT 
GGAACTGACA CAGCCTCTGG ACAAAGCAAT TCTGGAAACA ATGGGAACAA TGGAAAAGAT 
AGAGAGGACT CCTGGAAAGG AGCTTCTGTT CAGAAATCAT CTGGGTCAAA AAATGACTCT 
TGGGACAATA ACAGGTCTAC GGGTGGGTCC TGGAACTTTG GCCCTCAGGA CTCTAATGAC 
AACAAATGGG GTGAAGGGAA CAAAATGACA TCTGGGGTCT CTCAGGGAGA ATGGAAACAG 
CCGACTGGGT CTGATGAGTT GAAAATTGGA GAATGGAGTG GTCCAAACCA ACCAAATTCT 
AGCACTGGAG CATGGGACAA TCAAAAGGGC CACCCCCTCC CTGAAAACCA AGGCAATGCC 
CAGGCTCCCT GTTGGGGAAG ATCTTCCAGC TCCACAGGAA GTGAAGTTGG AGGTCAAAGC 
ACTGGAAGCA ACCACAAAGC AGGAAGTAGT GACAGTCATA ATTCTGGCCG TCGGTCATAC 
CGGCCCACAC ATCCTGATTG TCAGGCTGTC TTACAGACTC TTTTGAGCCG AACTGATTTG 
GACCCCAGGG TGCTCTCAAA CACTGGCTGG GGCCAAACTC AAATTAAGCA GGACACTGTG 
TGGGATATTG AAGAGGTGCC AAGGCCTGAG GGGAAATCGG ACAAAGGAAC TGAGGGGTGG 
GAGAGCGCTG CCACACAGAC CAAGAACTCA GGGGGCTGGG GAGATGCACC CAGCCAAAGC 
AATCAAATGA AGTCTGGATG GGGGGAGCTC TCAGCCTCTA CAGAGTGGAA AGACCCCAAG 
AACACAGGAG GCTGGAATGA CTATAAGAAC AACAACTCTT CCAACTGGGG AGGCGGACGA 
CCAGATGAAA AGACATCTTC CTCTTGGAAT GAGAACTCCA GCAAGGATCA GGGGTGGGGA 
GGGGGACGCC AGCCCAATCA AGGATGGACT TCTGGGAAGA ATGGTTGGGG AGAGGAAGTT 
GATCAGGCGA AAAACAGCAG TTGGGAAAGT TCTGCAAGCA AACCTGTGTC TGGATGGGGA 
GAAGGAGGGC AGAATGAAAT TGGAACTTGG GGGAACAGCG GGAATGCAAG CCTAGCTTCC 
AAAGGTGGGT GGGAAGATTG CAAAAGGTCT CCAGCGTGGA CTGAGACTGG CAGACAGCCC 
AATTCCTGGC ATAAACAGCA CCAACAGCAG CAGCAGCCAC AGCAGCCACC GCCACCACAA 
CCAGAGGCTT CTGGTTCATG GGGAGGCCCC CCACCACCAC CTCCAGGCAA CGGACGACCT 
TCCAATTCCA ACTGGAGCAG TGGGCCACAG CCAGTAACCC CTAAGGACGA GGAACCCAGT 
GGTTGGGAAG AGCCATCCCC ACAGTCAATT AGTCGGAAAA TGGACATCGA TGATGGCACT 
TCAGCATGGG GAGACCCTAA CAGCTATAAC TACAAGAATG TGAATCTGTG GGATAAGAAT 
TCCCAAGGGG GCCCAGCACC TCGAGAACCA AACCTGCCTA CCCCGATGAC CAGTAAATCA 
GCATCAGTCT GGAGCAAAAG CACACCACCT GCTCCAGATA ATGGTACTTC AGCTTGGGGT 
GAGCCAAACG AAAGCAGTCC TGGGTGGGGC GAAATGGATG ACACGGGAGC ATCGACCACG 
GGCTGGGGGA ATGCACCCTC CAACGCTCCA AATGCCATGA AACCTAATTC CAAATCTATG 
CAAGACGGCT GGGGGGAGAG TGACGGTCCA GTGACGGGAA CTCGCCATCC CAGCTGGGAG 
GAGGAGGATG ATGGAGGAGT CTGGAACACC GCCGGCTCTC AGGGAAGTGC TTCCTCCCAC 
AACTCAGCAA GCTGGGGACA AGGAGGAAAG AAGCAAATGA AGTGCTCACT GAAAGGAGGA 
AACAATGATT CATGGATGAA TCCTCTTGCC AAACAGTTTT CAAATATGGG ATTGCTGAGT 
CAAACTGAAG ATAATCCAGG CAGCAAAATG GATTTGTCTG TAGGGAGCCT TCCAGATAAA 
AAATTTGACG TGGACAAGCG AGCAATGAAT CTCGGGGATT TTAATGATAT CATGAGGAAG 
GATCGATCTG GGTTCCGTCC ACCTAATTCC AAAGACATGG GAACCACAGA TAGTGGGCCT 
TATTTCGAGA AGCTGACTTT GCCTTTCTCC AATCAAGATG GGTGCCTTGG GGATGAGGCT 
CCCTGCTCTC CCTTCTCCCC TTCTCCCAGC TACAAGCTGT CTCCCTCTGG TTCCACACTA 
CCCAACGTCA GCCTTGGAGC AATCGGCACA GGGCTCAACC CCCAAAACTT CGCTGCTAGA 
CAAGGTGGCA ATCATGGTTT GTTTGGAAAT AGCACAGCAC AATCGAGAGG TATGCACACA 
CCAGTGCAGC CACTAAATTC TTCTCCTAAT CTCCGGGCGC AAGTGCCTCC CCAGTTTATT 
TCCCCCCAGG TTTCTGCCTC CATGCTCAAG CAGTTTCCCA ACAGTGGCCT GAATCCAGGT 
CTTTTCAATG TGGGGCCCCA GTTATCTCCT CAACAGATTG CCATGCTGAG CCAGCTTCCA 
CAAATTCCCC AGTTTCAGTT GGCATGTCAG CTTCTCCTGC AGCAGCAGCA ACAGCAGCAG 
TTGCTACAGA ACCAGAGGAA GCTTTCTCAA GCTGTGCGCC AGCAGCAAGA GCAGCAGCTG 
GCTCGAATGG TGAGCGCTCT CCAGCAACAG CAGCAACAGC AGCAGCAGCA GCAGCAGAGG 
CAGCCTGGCA TGAAGCACTC ACCCTCTCAT CCTGTTGGGC CCAAGCCACA TCTGGACAAC 
ATGGTACCCA ACGCTCTGAA TGTGGGGCTC CCAGACCTTC AAACCAAAGG GCCAATACCT 
GGATATGGTT CTGGCTTCAG CTCTGGCGGC ATGGACTATG GCATGGTTGG TGGGAAGGAG 
GCTGGAACAG AGTCTCGCTT TAAACAGTGG ACCTCCATGA TGGAGGGACT GCCCTCTGTA 
GCCACACAGG AAGCCAATAT GCACAAAAAT GGCGCTATTG TGGCCCCTGG TAAGACTCGA 
GGAGGGTCAC CGTACAACCA GTTTGATATC ATCCCGAGTG ACACACTGGG TGGCCATACG 
GGTCCTGCTG GTGATAGCTG GTTACCTGCC AAATCTCCAC CAACAAATAA AATCGGAAGT 
AAATCCAGCA ATGCCAGTTG GCCTCCAGAA TTCCAACCAG GAGTGCCATG GAAAGGTATC 
CAAAACATTG ACCCTGAATC TGACCCCTAT GTCACCCCAG GAAGTGTACT GGGGGGTACA 
GCCACATCTC CCATTGTAGA TACTGACCAC CAACTTCTGC GGGATAACAC CACAGGGTCT 
AATTCTTCCC TCAACACCTC GCTGCCTTCA CCTGGTGCCT GGCCCTACAG TGCCTCTGAC 
AACTCCTTTA CCAACGTTCA TAGCACTTCA GCAAAGTTCC CTGATTACAA ATCAACATGG 
TCCCCAGATC CCATAGGACA CAACCCCACT CATCTCTCCA ACAAGATGTG GAAAAACCAT 
ATTTCCTCAA GGAACACTAC ACCGCTGCCC CGCCCACCTC CCGGTCTGAC CAACCCCAAA 
CCATCATCTC CCTGGAGCAG CACAGCACCC CGATCAGTCA GGGGGTGGGG GACACAGGAC 
TCACGGCTTG CCTCGGCCTC TACCTGGAGT GATGGTGGTT CAGTTCGTCC CAGTTACTGG 
CTGGTTCTTC ACAATCTCAC TCCACAGATT GATGGGTCAA CGTTGAGAAC CATCTGCATG 
CAGCATGGCC CACTGCTGAC ATTCCATCTG AACCTAACCC AGGGCACTGC CCTGATCCGA 
TACAGCACCA AACAGGAGGC GGCCAAGGCC CAAACTGCAC TGCACATGTG TGTGTTGGGA 
AACACTACCA TCCTGGCTGA GTTTGCCACC GATGATGAAG TTAGCCGCTT TCTGGCACAA 
GCTCAGCCCC CTACACCTGC AGCAACCCCA AGTGCACCCG CCGCAGGTTG GCAGTCGCTG 
GAGACCGGCC AGACCCAGTC AGATCCCGTC GGACCTGCTT TGAATCTTTT TGGTGGGTCC 
ACAGGGCTCG GGCAATGGAG CAGCAGTGCT GGTGGCAGCA GCGGGGCCGA TCTTGCTGGC 
GCTTCGTTGT GGGGGCCCCC AAACTATTCT TCTAGCTTGT GGGGAGTCCC AGCTGTGGAA 
GATCCCCATA GGATGGGCAG CCCTGCTCCC TTACTACCTG GTGACCTTCT GGGAGGAGGG 
TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280098.1
CDS	168..5822
Translation	MLKMLAPIFLQGAVIQNPEGLLVEREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKAPDILKHAVNHTLILGCLKFGLQEAVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424390.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424390.1

ORF Insert Sequence:

ATGCTGAAAA TGCTGGCCCC TATCTTTCTG CAGGGTGCAG TGATCCAGAA TCCAGAAGGG 
CTGTTAGTTG AGAGAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAAAAAGAA GCCACTCAGA AGGTCACGGA ACAAAAAACC 
AAAGCACCTG ACATTCTGAA GCATGCTGTG AACCACACAC TGATTCTGGG ATGTCTTAAG 
TTTGGACTTC AAGAAGCAGT GCCCGAAGTG ACGAAACCAA GTTTAAGCCA ACCAACGGCC 
GCCAGCCCAA TTGGCAGCTC TCCATCGCCA CCAGTCAATG GTGGCAACAA TGCCAAAAGG 
GTGGCAGTGC CGAACGGACA ACCGCCAAGC GCCGCCCGCT ACATGCCTCG GGAGGTGCCG 
CCGCGATTCC GTTGCCAGCA GGACCACAAA GTGTTACTAA AACGTGGGCA GCCCCCTCCG 
CCGTCCTGCA TGCTCCTTGG GGGCGGAGCA GGGCCTCCTC CCTCCACAGC ACCTGGAGCA 
AACTCAAACA ACGCACAAGT GACAGGAACG CTGCTGCAGA GTGACAGTGG GACTGCACCA 
GAGTCAACCC TTGGAGGTGC TGCTGCTTCA AATTATGCAA ATTCCACTTG GGGCCCGGGA 
GCCTCCTCCA ACAACGGCGC CTCCCCCAAC CCAATTCACA CCTGGGACAA GGTGATTGTA 
GACGGGTCTG ACGTGGAAGA GTGGCCTTGT ATTGCCAGCA AAGACACTGA GTCTTCTTCC 
GAAAACACCA CCGATAACAA CAGTGCCTCG AACCCTGGCT CTGAGAAGAG CACTCTGCCA 
GGAAGCACCA CTAGTAACAA AGGAAAAGGG AGCCAGTGCC AGTCTGCAAG TTCTGGGAAC 
GAATGTAATC TTGGGGTCTG GAAATCTGAC CCTAAGACTA AATCTGTTCA ATCTTCCAAC 
TCTACAACAG AGAGCAACAA TGGACTAGGA AATTGGAGGA ATGTGAGTGG TCAGGATAGA 
ATTGGACCTG GCTCTGGCTT CAGCAACTTT AACCCAAATA GCAACCCATC TGCCTGGCCA 
GCGCTGGTCC AAGAAGGAAA TTCTAGGAAA GGGGCATTGG AAACGGATAC TAGTAATTCC 
AGTGCACAGG TTAGCACAGT AGGTCAGGCA TCCAGGGAAC AGCAGTCAAA GATGGAAAAT 
GCGGGTGTTA ATTTTGTTGT CTCTGGCAGA GAACAGGCTC AGATTCATAA CACTGATGGA 
CCAAAAAATG GAAACACTAA CTCCTTGAAC TTAAGTTCAC CAAACCCCAT GGAGAATAAG 
GGAATGCCCT TTGGAATGGG CTTGGGGAAC GCCTCCAGGA GCACTGATGC CCCTTCACAA 
AGCACTGGAG ATCGAAAGAC TGGGAGTGTT GGATCTTGGG GTGCAGCTAG GGGGCCTTCT 
GGAACTGACA CAGCCTCTGG ACAAAGCAAT TCTGGAAACA ATGGGAACAA TGGAAAAGAT 
AGAGAGGACT CCTGGAAAGG AGCTTCTGTT CAGAAATCAT CTGGGTCAAA AAATGACTCT 
TGGGACAATA ACAGGTCTAC GGGTGGGTCC TGGAACTTTG GCCCTCAGGA CTCTAATGAC 
AACAAATGGG GTGAAGGGAA CAAAATGACA TCTGGGGTCT CTCAGGGAGA ATGGAAACAG 
CCGACTGGGT CTGATGAGTT GAAAATTGGA GAATGGAGTG GTCCAAACCA ACCAAATTCT 
AGCACTGGAG CATGGGACAA TCAAAAGGGC CACCCCCTCC CTGAAAACCA AGGCAATGCC 
CAGGCTCCCT GTTGGGGAAG ATCTTCCAGC TCCACAGGAA GTGAAGTTGG AGGTCAAAGC 
ACTGGAAGCA ACCACAAAGC AGGAAGTAGT GACAGTCATA ATTCTGGCCG TCGGTCATAC 
CGGCCCACAC ATCCTGATTG TCAGGCTGTC TTACAGACTC TTTTGAGCCG AACTGATTTG 
GACCCCAGGG TGCTCTCAAA CACTGGCTGG GGCCAAACTC AAATTAAGCA GGACACTGTG 
TGGGATATTG AAGAGGTGCC AAGGCCTGAG GGGAAATCGG ACAAAGGAAC TGAGGGGTGG 
GAGAGCGCTG CCACACAGAC CAAGAACTCA GGGGGCTGGG GAGATGCACC CAGCCAAAGC 
AATCAAATGA AGTCTGGATG GGGGGAGCTC TCAGCCTCTA CAGAGTGGAA AGACCCCAAG 
AACACAGGAG GCTGGAATGA CTATAAGAAC AACAACTCTT CCAACTGGGG AGGCGGACGA 
CCAGATGAAA AGACATCTTC CTCTTGGAAT GAGAACTCCA GCAAGGATCA GGGGTGGGGA 
GGGGGACGCC AGCCCAATCA AGGATGGACT TCTGGGAAGA ATGGTTGGGG AGAGGAAGTT 
GATCAGGCGA AAAACAGCAG TTGGGAAAGT TCTGCAAGCA AACCTGTGTC TGGATGGGGA 
GAAGGAGGGC AGAATGAAAT TGGAACTTGG GGGAACAGCG GGAATGCAAG CCTAGCTTCC 
AAAGGTGGGT GGGAAGATTG CAAAAGGTCT CCAGCGTGGA CTGAGACTGG CAGACAGCCC 
AATTCCTGGC ATAAACAGCA CCAACAGCAG CAGCAGCCAC AGCAGCCACC GCCACCACAA 
CCAGAGGCTT CTGGTTCATG GGGAGGCCCC CCACCACCAC CTCCAGGCAA CGGACGACCT 
TCCAATTCCA ACTGGAGCAG TGGGCCACAG CCAGTAACCC CTAAGGACGA GGAACCCAGT 
GGTTGGGAAG AGCCATCCCC ACAGTCAATT AGTCGGAAAA TGGACATCGA TGATGGCACT 
TCAGCATGGG GAGACCCTAA CAGCTATAAC TACAAGAATG TGAATCTGTG GGATAAGAAT 
TCCCAAGGGG GCCCAGCACC TCGAGAACCA AACCTGCCTA CCCCGATGAC CAGTAAATCA 
GCATCAGTCT GGAGCAAAAG CACACCACCT GCTCCAGATA ATGGTACTTC AGCTTGGGGT 
GAGCCAAACG AAAGCAGTCC TGGGTGGGGC GAAATGGATG ACACGGGAGC ATCGACCACG 
GGCTGGGGGA ATGCACCCTC CAACGCTCCA AATGCCATGA AACCTAATTC CAAATCTATG 
CAAGACGGCT GGGGGGAGAG TGACGGTCCA GTGACGGGAA CTCGCCATCC CAGCTGGGAG 
GAGGAGGATG ATGGAGGAGT CTGGAACACC GCCGGCTCTC AGGGAAGTGC TTCCTCCCAC 
AACTCAGCAA GCTGGGGACA AGGAGGAAAG AAGCAAATGA AGTGCTCACT GAAAGGAGGA 
AACAATGATT CATGGATGAA TCCTCTTGCC AAACAGTTTT CAAATATGGG ATTGCTGAGT 
CAAACTGAAG ATAATCCAGG CAGCAAAATG GATTTGTCTG TAGGGAGCCT TCCAGATAAA 
AAATTTGACG TGGACAAGCG AGCAATGAAT CTCGGGGATT TTAATGATAT CATGAGGAAG 
GATCGATCTG GGTTCCGTCC ACCTAATTCC AAAGACATGG GAACCACAGA TAGTGGGCCT 
TATTTCGAGA AGCTGACTTT GCCTTTCTCC AATCAAGATG GGTGCCTTGG GGATGAGGCT 
CCCTGCTCTC CCTTCTCCCC TTCTCCCAGC TACAAGCTGT CTCCCTCTGG TTCCACACTA 
CCCAACGTCA GCCTTGGAGC AATCGGCACA GGGCTCAACC CCCAAAACTT CGCTGCTAGA 
CAAGGTGGCA ATCATGGTTT GTTTGGAAAT AGCACAGCAC AATCGAGAGG TATGCACACA 
CCAGTGCAGC CACTAAATTC TTCTCCTAAT CTCCGGGCGC AAGTGCCTCC CCAGTTTATT 
TCCCCCCAGG TTTCTGCCTC CATGCTCAAG CAGTTTCCCA ACAGTGGCCT GAATCCAGGT 
CTTTTCAATG TGGGGCCCCA GTTATCTCCT CAACAGATTG CCATGCTGAG CCAGCTTCCA 
CAAATTCCCC AGTTTCAGTT GGCATGTCAG CTTCTCCTGC AGCAGCAGCA ACAGCAGCAG 
TTGCTACAGA ACCAGAGGAA GCTTTCTCAA GCTGTGCGCC AGCAGCAAGA GCAGCAGCTG 
GCTCGAATGG TGAGCGCTCT CCAGCAACAG CAGCAACAGC AGCAGCAGCA GCAGCAGAGG 
CAGCCTGGCA TGAAGCACTC ACCCTCTCAT CCTGTTGGGC CCAAGCCACA TCTGGACAAC 
ATGGTACCCA ACGCTCTGAA TGTGGGGCTC CCAGACCTTC AAACCAAAGG GCCAATACCT 
GGATATGGTT CTGGCTTCAG CTCTGGCGGC ATGGACTATG GCATGGTTGG TGGGAAGGAG 
GCTGGAACAG AGTCTCGCTT TAAACAGTGG ACCTCCATGA TGGAGGGACT GCCCTCTGTA 
GCCACACAGG AAGCCAATAT GCACAAAAAT GGCGCTATTG TGGCCCCTGG TAAGACTCGA 
GGAGGGTCAC CGTACAACCA GTTTGATATC ATCCCGAGTG ACACACTGGG TGGCCATACG 
GGTCCTGCTG GTGATAGCTG GTTACCTGCC AAATCTCCAC CAACAAATAA AATCGGAAGT 
AAATCCAGCA ATGCCAGTTG GCCTCCAGAA TTCCAACCAG GAGTGCCATG GAAAGGTATC 
CAAAACATTG ACCCTGAATC TGACCCCTAT GTCACCCCAG GAAGTGTACT GGGGGGTACA 
GCCACATCTC CCATTGTAGA TACTGACCAC CAACTTCTGC GGGATAACAC CACAGGGTCT 
AATTCTTCCC TCAACACCTC GCTGCCTTCA CCTGGTGCCT GGCCCTACAG TGCCTCTGAC 
AACTCCTTTA CCAACGTTCA TAGCACTTCA GCAAAGTTCC CTGATTACAA ATCAACATGG 
TCCCCAGATC CCATAGGACA CAACCCCACT CATCTCTCCA ACAAGATGTG GAAAAACCAT 
ATTTCCTCAA GGAACACTAC ACCGCTGCCC CGCCCACCTC CCGGTCTGAC CAACCCCAAA 
CCATCATCTC CCTGGAGCAG CACAGCACCC CGATCAGTCA GGGGGTGGGG GACACAGGAC 
TCACGGCTTG CCTCGGCCTC TACCTGGAGT GATGGTGGTT CAGTTCGTCC CAGTTACTGG 
CTGGTTCTTC ACAATCTCAC TCCACAGATT GATGGGTCAA CGTTGAGAAC CATCTGCATG 
CAGCATGGCC CACTGCTGAC ATTCCATCTG AACCTAACCC AGGGCACTGC CCTGATCCGA 
TACAGCACCA AACAGGAGGC GGCCAAGGCC CAAACTGCAC TGCACATGTG TGTGTTGGGA 
AACACTACCA TCCTGGCTGA GTTTGCCACC GATGATGAAG TTAGCCGCTT TCTGGCACAA 
GCTCAGCCCC CTACACCTGC AGCAACCCCA AGTGCACCCG CCGCAGGTTG GCAGTCGCTG 
GAGACCGGCC AGACCCAGTC AGATCCCGTC GGACCTGCTT TGAATCTTTT TGGTGGGTCC 
ACAGGGCTCG GGCAATGGAG CAGCAGTGCT GGTGGCAGCA GCGGGGCCGA TCTTGCTGGC 
GCTTCGTTGT GGGGGCCCCC AAACTATTCT TCTAGCTTGT GGGGAGTCCC AGCTGTGGAA 
GATCCCCATA GGATGGGCAG CCCTGCTCCC TTACTACCTG GTGACCTTCT GGGAGGAGGG 
TCGGATTCAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424391.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280099.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

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CloneID	OCe291431
Clone ID Related Accession (Same CDS sequence)	XM_022424391.1
Accession Version	XM_022424391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC ATCAGGATTG CTTCTGGCTG TATGACCCAC CTTCCTCTTT CCATCAATCC 
ATGTCCCACC ATTTTCCATT CCATAACAGA AGTCAGCTTC ATGAGAATAC TCTTTGCTTC 
TACTTTAATC TCTTCCAGGG TGCAGTGATC CAGAATCCAG AAGGGCTGTT AGTTGAGAGA 
GAGAAGGAGC AAGAAAGGGA AGAACAGTTA ATGGAAGACA AGAAAAGGAA GAAAGAGGAT 
AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC ACGGAACAAA AAACCAAAGC ACCTGACATT 
CTGAAGCATG CTGTGAACCA CACACTGATT CTGGGATGTC TTAAGTTTGG ACTTCAAGAA 
GCAGTGCCCG AAGTGACGAA ACCAAGTTTA AGCCAACCAA CGGCCGCCAG CCCAATTGGC 
AGCTCTCCAT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CAAGCGCCGC CCGCTACATG CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC 
CAGCAGGACC ACAAAGTGTT ACTAAAACGT GGGCAGCCCC CTCCGCCGTC CTGCATGCTC 
CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC ACAGCACCTG GAGCAAACTC AAACAACGCA 
CAAGTGACAG GAACGCTGCT GCAGAGTGAC AGTGGGACTG CACCAGAGTC AACCCTTGGA 
GGTGCTGCTG CTTCAAATTA TGCAAATTCC ACTTGGGGCC CGGGAGCCTC CTCCAACAAC 
GGCGCCTCCC CCAACCCAAT TCACACCTGG GACAAGGTGA TTGTAGACGG GTCTGACGTG 
GAAGAGTGGC CTTGTATTGC CAGCAAAGAC ACTGAGTCTT CTTCCGAAAA CACCACCGAT 
AACAACAGTG CCTCGAACCC TGGCTCTGAG AAGAGCACTC TGCCAGGAAG CACCACTAGT 
AACAAAGGAA AAGGGAGCCA GTGCCAGTCT GCAAGTTCTG GGAACGAATG TAATCTTGGG 
GTCTGGAAAT CTGACCCTAA GACTAAATCT GTTCAATCTT CCAACTCTAC AACAGAGAGC 
AACAATGGAC TAGGAAATTG GAGGAATGTG AGTGGTCAGG ATAGAATTGG ACCTGGCTCT 
GGCTTCAGCA ACTTTAACCC AAATAGCAAC CCATCTGCCT GGCCAGCGCT GGTCCAAGAA 
GGAAATTCTA GGAAAGGGGC ATTGGAAACG GATACTAGTA ATTCCAGTGC ACAGGTTAGC 
ACAGTAGGTC AGGCATCCAG GGAACAGCAG TCAAAGATGG AAAATGCGGG TGTTAATTTT 
GTTGTCTCTG GCAGAGAACA GGCTCAGATT CATAACACTG ATGGACCAAA AAATGGAAAC 
ACTAACTCCT TGAACTTAAG TTCACCAAAC CCCATGGAGA ATAAGGGAAT GCCCTTTGGA 
ATGGGCTTGG GGAACGCCTC CAGGAGCACT GATGCCCCTT CACAAAGCAC TGGAGATCGA 
AAGACTGGGA GTGTTGGATC TTGGGGTGCA GCTAGGGGGC CTTCTGGAAC TGACACAGCC 
TCTGGACAAA GCAATTCTGG AAACAATGGG AACAATGGAA AAGATAGAGA GGACTCCTGG 
AAAGGAGCTT CTGTTCAGAA ATCATCTGGG TCAAAAAATG ACTCTTGGGA CAATAACAGG 
TCTACGGGTG GGTCCTGGAA CTTTGGCCCT CAGGACTCTA ATGACAACAA ATGGGGTGAA 
GGGAACAAAA TGACATCTGG GGTCTCTCAG GGAGAATGGA AACAGCCGAC TGGGTCTGAT 
GAGTTGAAAA TTGGAGAATG GAGTGGTCCA AACCAACCAA ATTCTAGCAC TGGAGCATGG 
GACAATCAAA AGGGCCACCC CCTCCCTGAA AACCAAGGCA ATGCCCAGGC TCCCTGTTGG 
GGAAGATCTT CCAGCTCCAC AGGAAGTGAA GTTGGAGGTC AAAGCACTGG AAGCAACCAC 
AAAGCAGGAA GTAGTGACAG TCATAATTCT GGCCGTCGGT CATACCGGCC CACACATCCT 
GATTGTCAGG CTGTCTTACA GACTCTTTTG AGCCGAACTG ATTTGGACCC CAGGGTGCTC 
TCAAACACTG GCTGGGGCCA AACTCAAATT AAGCAGGACA CTGTGTGGGA TATTGAAGAG 
GTGCCAAGGC CTGAGGGGAA ATCGGACAAA GGAACTGAGG GGTGGGAGAG CGCTGCCACA 
CAGACCAAGA ACTCAGGGGG CTGGGGAGAT GCACCCAGCC AAAGCAATCA AATGAAGTCT 
GGATGGGGGG AGCTCTCAGC CTCTACAGAG TGGAAAGACC CCAAGAACAC AGGAGGCTGG 
AATGACTATA AGAACAACAA CTCTTCCAAC TGGGGAGGCG GACGACCAGA TGAAAAGACA 
TCTTCCTCTT GGAATGAGAA CTCCAGCAAG GATCAGGGGT GGGGAGGGGG ACGCCAGCCC 
AATCAAGGAT GGACTTCTGG GAAGAATGGT TGGGGAGAGG AAGTTGATCA GGCGAAAAAC 
AGCAGTTGGG AAAGTTCTGC AAGCAAACCT GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT 
GAAATTGGAA CTTGGGGGAA CAGCGGGAAT GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA 
GATTGCAAAA GGTCTCCAGC GTGGACTGAG ACTGGCAGAC AGCCCAATTC CTGGCATAAA 
CAGCACCAAC AGCAGCAGCA GCCACAGCAG CCACCGCCAC CACAACCAGA GGCTTCTGGT 
TCATGGGGAG GCCCCCCACC ACCACCTCCA GGCAACGGAC GACCTTCCAA TTCCAACTGG 
AGCAGTGGGC CACAGCCAGT AACCCCTAAG GACGAGGAAC CCAGTGGTTG GGAAGAGCCA 
TCCCCACAGT CAATTAGTCG GAAAATGGAC ATCGATGATG GCACTTCAGC ATGGGGAGAC 
CCTAACAGCT ATAACTACAA GAATGTGAAT CTGTGGGATA AGAATTCCCA AGGGGGCCCA 
GCACCTCGAG AACCAAACCT GCCTACCCCG ATGACCAGTA AATCAGCATC AGATTCCAAA 
TCTATGCAAG ACGGCTGGGG GGAGAGTGAC GGTCCAGTGA CGGGAACTCG CCATCCCAGC 
TGGGAGGAGG AGGATGATGG AGGAGTCTGG AACACCGCCG GCTCTCAGGG AAGTGCTTCC 
TCCCACAACT CAGCAAGCTG GGGACAAGGA GGAAAGAAGC AAATGAAGTG CTCACTGAAA 
GGAGGAAACA ATGATTCATG GATGAATCCT CTTGCCAAAC AGTTTTCAAA TATGGGATTG 
CTGAGTCAAA CTGAAGATAA TCCAGGCAGC AAAATGGATT TGTCTGTAGG GAGCCTTCCA 
GATAAAAAAT TTGACGTGGA CAAGCGAGCA ATGAATCTCG GGGATTTTAA TGATATCATG 
AGGAAGGATC GATCTGGGTT CCGTCCACCT AATTCCAAAG ACATGGGAAC CACAGATAGT 
GGGCCTTATT TCGAGAAGCT GACTTTGCCT TTCTCCAATC AAGATGGGTG CCTTGGGGAT 
GAGGCTCCCT GCTCTCCCTT CTCCCCTTCT CCCAGCTACA AGCTGTCTCC CTCTGGTTCC 
ACACTACCCA ACGTCAGCCT TGGAGCAATC GGCACAGGGC TCAACCCCCA AAACTTCGCT 
GCTAGACAAG GTGGCAATCA TGGTTTGTTT GGAAATAGCA CAGCACAATC GAGAGGTATG 
CACACACCAG TGCAGCCACT AAATTCTTCT CCTAATCTCC GGGCGCAAGT GCCTCCCCAG 
TTTATTTCCC CCCAGGTTTC TGCCTCCATG CTCAAGCAGT TTCCCAACAG TGGCCTGAAT 
CCAGGTCTTT TCAATGTGGG GCCCCAGTTA TCTCCTCAAC AGATTGCCAT GCTGAGCCAG 
CTTCCACAAA TTCCCCAGTT TCAGTTGGCA TGTCAGCTTC TCCTGCAGCA GCAGCAACAG 
CAGCAGTTGC TACAGAACCA GAGGAAGCTT TCTCAAGCTG TGCGCCAGCA GCAAGAGCAG 
CAGCTGGCTC GAATGGTGAG CGCTCTCCAG CAACAGCAGC AACAGCAGCA GCAGCAGCAG 
CAGAGGCAGC CTGGCATGAA GCACTCACCC TCTCATCCTG TTGGGCCCAA GCCACATCTG 
GACAACATGG TACCCAACGC TCTGAATGTG GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA 
ATACCTGGAT ATGGTTCTGG CTTCAGCTCT GGCGGCATGG ACTATGGCAT GGTTGGTGGG 
AAGGAGGCTG GAACAGAGTC TCGCTTTAAA CAGTGGACCT CCATGATGGA GGGACTGCCC 
TCTGTAGCCA CACAGGAAGC CAATATGCAC AAAAATGGCG CTATTGTGGC CCCTGGTAAG 
ACTCGAGGAG GGTCACCGTA CAACCAGTTT GATATCATCC CGAGTGACAC ACTGGGTGGC 
CATACGGGTC CTGCTGGTGA TAGCTGGTTA CCTGCCAAAT CTCCACCAAC AAATAAAATC 
GGAAGTAAAT CCAGCAATGC CAGTTGGCCT CCAGAATTCC AACCAGGAGT GCCATGGAAA 
GGTATCCAAA ACATTGACCC TGAATCTGAC CCCTATGTCA CCCCAGGAAG TGTACTGGGG 
GGTACAGCCA CATCTCCCAT TGTAGATACT GACCACCAAC TTCTGCGGGA TAACACCACA 
GGGTCTAATT CTTCCCTCAA CACCTCGCTG CCTTCACCTG GTGCCTGGCC CTACAGTGCC 
TCTGACAACT CCTTTACCAA CGTTCATAGC ACTTCAGCAA AGTTCCCTGA TTACAAATCA 
ACATGGTCCC CAGATCCCAT AGGACACAAC CCCACTCATC TCTCCAACAA GATGTGGAAA 
AACCATATTT CCTCAAGGAA CACTACACCG CTGCCCCGCC CACCTCCCGG TCTGACCAAC 
CCCAAACCAT CATCTCCCTG GAGCAGCACA GCACCCCGAT CAGTCAGGGG GTGGGGGACA 
CAGGACTCAC GGCTTGCCTC GGCCTCTACC TGGAGTGATG GTGGTTCAGT TCGTCCCAGT 
TACTGGCTGG TTCTTCACAA TCTCACTCCA CAGATTGATG GGTCAACGTT GAGAACCATC 
TGCATGCAGC ATGGCCCACT GCTGACATTC CATCTGAACC TAACCCAGGG CACTGCCCTG 
ATCCGATACA GCACCAAACA GGAGGCGGCC AAGGCCCAAA CTGCACTGCA CATGTGTGTG 
TTGGGAAACA CTACCATCCT GGCTGAGTTT GCCACCGATG ATGAAGTTAG CCGCTTTCTG 
GCACAAGCTC AGCCCCCTAC ACCTGCAGCA ACCCCAAGTG CACCCGCCGC AGGTTGGCAG 
TCGCTGGAGA CCGGCCAGAC CCAGTCAGAT CCCGTCGGAC CTGCTTTGAA TCTTTTTGGT 
GGGTCCACAG GGCTCGGGCA ATGGAGCAGC AGTGCTGGTG GCAGCAGCGG GGCCGATCTT 
GCTGGCGCTT CGTTGTGGGG GCCCCCAAAC TATTCTTCTA GCTTGTGGGG AGTCCCAGCT 
GTGGAAGATC CCCATAGGAT GGGCAGCCCT GCTCCCTTAC TACCTGGTGA CCTTCTGGGA 
GGAGGGTCGG ATTCAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280099.1
CDS	16..5616
Translation	MDSHQDCFWLYDPPSSFHQSMSHHFPFHNRSQLHENTLCFYFNLFQGAVIQNPEGLLVEREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKAPDILKHAVNHTLILGCLKFGLQEAVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASDSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424391.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424391.1

ORF Insert Sequence:

ATGGACTCCC ATCAGGATTG CTTCTGGCTG TATGACCCAC CTTCCTCTTT CCATCAATCC 
ATGTCCCACC ATTTTCCATT CCATAACAGA AGTCAGCTTC ATGAGAATAC TCTTTGCTTC 
TACTTTAATC TCTTCCAGGG TGCAGTGATC CAGAATCCAG AAGGGCTGTT AGTTGAGAGA 
GAGAAGGAGC AAGAAAGGGA AGAACAGTTA ATGGAAGACA AGAAAAGGAA GAAAGAGGAT 
AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC ACGGAACAAA AAACCAAAGC ACCTGACATT 
CTGAAGCATG CTGTGAACCA CACACTGATT CTGGGATGTC TTAAGTTTGG ACTTCAAGAA 
GCAGTGCCCG AAGTGACGAA ACCAAGTTTA AGCCAACCAA CGGCCGCCAG CCCAATTGGC 
AGCTCTCCAT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CAAGCGCCGC CCGCTACATG CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC 
CAGCAGGACC ACAAAGTGTT ACTAAAACGT GGGCAGCCCC CTCCGCCGTC CTGCATGCTC 
CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC ACAGCACCTG GAGCAAACTC AAACAACGCA 
CAAGTGACAG GAACGCTGCT GCAGAGTGAC AGTGGGACTG CACCAGAGTC AACCCTTGGA 
GGTGCTGCTG CTTCAAATTA TGCAAATTCC ACTTGGGGCC CGGGAGCCTC CTCCAACAAC 
GGCGCCTCCC CCAACCCAAT TCACACCTGG GACAAGGTGA TTGTAGACGG GTCTGACGTG 
GAAGAGTGGC CTTGTATTGC CAGCAAAGAC ACTGAGTCTT CTTCCGAAAA CACCACCGAT 
AACAACAGTG CCTCGAACCC TGGCTCTGAG AAGAGCACTC TGCCAGGAAG CACCACTAGT 
AACAAAGGAA AAGGGAGCCA GTGCCAGTCT GCAAGTTCTG GGAACGAATG TAATCTTGGG 
GTCTGGAAAT CTGACCCTAA GACTAAATCT GTTCAATCTT CCAACTCTAC AACAGAGAGC 
AACAATGGAC TAGGAAATTG GAGGAATGTG AGTGGTCAGG ATAGAATTGG ACCTGGCTCT 
GGCTTCAGCA ACTTTAACCC AAATAGCAAC CCATCTGCCT GGCCAGCGCT GGTCCAAGAA 
GGAAATTCTA GGAAAGGGGC ATTGGAAACG GATACTAGTA ATTCCAGTGC ACAGGTTAGC 
ACAGTAGGTC AGGCATCCAG GGAACAGCAG TCAAAGATGG AAAATGCGGG TGTTAATTTT 
GTTGTCTCTG GCAGAGAACA GGCTCAGATT CATAACACTG ATGGACCAAA AAATGGAAAC 
ACTAACTCCT TGAACTTAAG TTCACCAAAC CCCATGGAGA ATAAGGGAAT GCCCTTTGGA 
ATGGGCTTGG GGAACGCCTC CAGGAGCACT GATGCCCCTT CACAAAGCAC TGGAGATCGA 
AAGACTGGGA GTGTTGGATC TTGGGGTGCA GCTAGGGGGC CTTCTGGAAC TGACACAGCC 
TCTGGACAAA GCAATTCTGG AAACAATGGG AACAATGGAA AAGATAGAGA GGACTCCTGG 
AAAGGAGCTT CTGTTCAGAA ATCATCTGGG TCAAAAAATG ACTCTTGGGA CAATAACAGG 
TCTACGGGTG GGTCCTGGAA CTTTGGCCCT CAGGACTCTA ATGACAACAA ATGGGGTGAA 
GGGAACAAAA TGACATCTGG GGTCTCTCAG GGAGAATGGA AACAGCCGAC TGGGTCTGAT 
GAGTTGAAAA TTGGAGAATG GAGTGGTCCA AACCAACCAA ATTCTAGCAC TGGAGCATGG 
GACAATCAAA AGGGCCACCC CCTCCCTGAA AACCAAGGCA ATGCCCAGGC TCCCTGTTGG 
GGAAGATCTT CCAGCTCCAC AGGAAGTGAA GTTGGAGGTC AAAGCACTGG AAGCAACCAC 
AAAGCAGGAA GTAGTGACAG TCATAATTCT GGCCGTCGGT CATACCGGCC CACACATCCT 
GATTGTCAGG CTGTCTTACA GACTCTTTTG AGCCGAACTG ATTTGGACCC CAGGGTGCTC 
TCAAACACTG GCTGGGGCCA AACTCAAATT AAGCAGGACA CTGTGTGGGA TATTGAAGAG 
GTGCCAAGGC CTGAGGGGAA ATCGGACAAA GGAACTGAGG GGTGGGAGAG CGCTGCCACA 
CAGACCAAGA ACTCAGGGGG CTGGGGAGAT GCACCCAGCC AAAGCAATCA AATGAAGTCT 
GGATGGGGGG AGCTCTCAGC CTCTACAGAG TGGAAAGACC CCAAGAACAC AGGAGGCTGG 
AATGACTATA AGAACAACAA CTCTTCCAAC TGGGGAGGCG GACGACCAGA TGAAAAGACA 
TCTTCCTCTT GGAATGAGAA CTCCAGCAAG GATCAGGGGT GGGGAGGGGG ACGCCAGCCC 
AATCAAGGAT GGACTTCTGG GAAGAATGGT TGGGGAGAGG AAGTTGATCA GGCGAAAAAC 
AGCAGTTGGG AAAGTTCTGC AAGCAAACCT GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT 
GAAATTGGAA CTTGGGGGAA CAGCGGGAAT GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA 
GATTGCAAAA GGTCTCCAGC GTGGACTGAG ACTGGCAGAC AGCCCAATTC CTGGCATAAA 
CAGCACCAAC AGCAGCAGCA GCCACAGCAG CCACCGCCAC CACAACCAGA GGCTTCTGGT 
TCATGGGGAG GCCCCCCACC ACCACCTCCA GGCAACGGAC GACCTTCCAA TTCCAACTGG 
AGCAGTGGGC CACAGCCAGT AACCCCTAAG GACGAGGAAC CCAGTGGTTG GGAAGAGCCA 
TCCCCACAGT CAATTAGTCG GAAAATGGAC ATCGATGATG GCACTTCAGC ATGGGGAGAC 
CCTAACAGCT ATAACTACAA GAATGTGAAT CTGTGGGATA AGAATTCCCA AGGGGGCCCA 
GCACCTCGAG AACCAAACCT GCCTACCCCG ATGACCAGTA AATCAGCATC AGATTCCAAA 
TCTATGCAAG ACGGCTGGGG GGAGAGTGAC GGTCCAGTGA CGGGAACTCG CCATCCCAGC 
TGGGAGGAGG AGGATGATGG AGGAGTCTGG AACACCGCCG GCTCTCAGGG AAGTGCTTCC 
TCCCACAACT CAGCAAGCTG GGGACAAGGA GGAAAGAAGC AAATGAAGTG CTCACTGAAA 
GGAGGAAACA ATGATTCATG GATGAATCCT CTTGCCAAAC AGTTTTCAAA TATGGGATTG 
CTGAGTCAAA CTGAAGATAA TCCAGGCAGC AAAATGGATT TGTCTGTAGG GAGCCTTCCA 
GATAAAAAAT TTGACGTGGA CAAGCGAGCA ATGAATCTCG GGGATTTTAA TGATATCATG 
AGGAAGGATC GATCTGGGTT CCGTCCACCT AATTCCAAAG ACATGGGAAC CACAGATAGT 
GGGCCTTATT TCGAGAAGCT GACTTTGCCT TTCTCCAATC AAGATGGGTG CCTTGGGGAT 
GAGGCTCCCT GCTCTCCCTT CTCCCCTTCT CCCAGCTACA AGCTGTCTCC CTCTGGTTCC 
ACACTACCCA ACGTCAGCCT TGGAGCAATC GGCACAGGGC TCAACCCCCA AAACTTCGCT 
GCTAGACAAG GTGGCAATCA TGGTTTGTTT GGAAATAGCA CAGCACAATC GAGAGGTATG 
CACACACCAG TGCAGCCACT AAATTCTTCT CCTAATCTCC GGGCGCAAGT GCCTCCCCAG 
TTTATTTCCC CCCAGGTTTC TGCCTCCATG CTCAAGCAGT TTCCCAACAG TGGCCTGAAT 
CCAGGTCTTT TCAATGTGGG GCCCCAGTTA TCTCCTCAAC AGATTGCCAT GCTGAGCCAG 
CTTCCACAAA TTCCCCAGTT TCAGTTGGCA TGTCAGCTTC TCCTGCAGCA GCAGCAACAG 
CAGCAGTTGC TACAGAACCA GAGGAAGCTT TCTCAAGCTG TGCGCCAGCA GCAAGAGCAG 
CAGCTGGCTC GAATGGTGAG CGCTCTCCAG CAACAGCAGC AACAGCAGCA GCAGCAGCAG 
CAGAGGCAGC CTGGCATGAA GCACTCACCC TCTCATCCTG TTGGGCCCAA GCCACATCTG 
GACAACATGG TACCCAACGC TCTGAATGTG GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA 
ATACCTGGAT ATGGTTCTGG CTTCAGCTCT GGCGGCATGG ACTATGGCAT GGTTGGTGGG 
AAGGAGGCTG GAACAGAGTC TCGCTTTAAA CAGTGGACCT CCATGATGGA GGGACTGCCC 
TCTGTAGCCA CACAGGAAGC CAATATGCAC AAAAATGGCG CTATTGTGGC CCCTGGTAAG 
ACTCGAGGAG GGTCACCGTA CAACCAGTTT GATATCATCC CGAGTGACAC ACTGGGTGGC 
CATACGGGTC CTGCTGGTGA TAGCTGGTTA CCTGCCAAAT CTCCACCAAC AAATAAAATC 
GGAAGTAAAT CCAGCAATGC CAGTTGGCCT CCAGAATTCC AACCAGGAGT GCCATGGAAA 
GGTATCCAAA ACATTGACCC TGAATCTGAC CCCTATGTCA CCCCAGGAAG TGTACTGGGG 
GGTACAGCCA CATCTCCCAT TGTAGATACT GACCACCAAC TTCTGCGGGA TAACACCACA 
GGGTCTAATT CTTCCCTCAA CACCTCGCTG CCTTCACCTG GTGCCTGGCC CTACAGTGCC 
TCTGACAACT CCTTTACCAA CGTTCATAGC ACTTCAGCAA AGTTCCCTGA TTACAAATCA 
ACATGGTCCC CAGATCCCAT AGGACACAAC CCCACTCATC TCTCCAACAA GATGTGGAAA 
AACCATATTT CCTCAAGGAA CACTACACCG CTGCCCCGCC CACCTCCCGG TCTGACCAAC 
CCCAAACCAT CATCTCCCTG GAGCAGCACA GCACCCCGAT CAGTCAGGGG GTGGGGGACA 
CAGGACTCAC GGCTTGCCTC GGCCTCTACC TGGAGTGATG GTGGTTCAGT TCGTCCCAGT 
TACTGGCTGG TTCTTCACAA TCTCACTCCA CAGATTGATG GGTCAACGTT GAGAACCATC 
TGCATGCAGC ATGGCCCACT GCTGACATTC CATCTGAACC TAACCCAGGG CACTGCCCTG 
ATCCGATACA GCACCAAACA GGAGGCGGCC AAGGCCCAAA CTGCACTGCA CATGTGTGTG 
TTGGGAAACA CTACCATCCT GGCTGAGTTT GCCACCGATG ATGAAGTTAG CCGCTTTCTG 
GCACAAGCTC AGCCCCCTAC ACCTGCAGCA ACCCCAAGTG CACCCGCCGC AGGTTGGCAG 
TCGCTGGAGA CCGGCCAGAC CCAGTCAGAT CCCGTCGGAC CTGCTTTGAA TCTTTTTGGT 
GGGTCCACAG GGCTCGGGCA ATGGAGCAGC AGTGCTGGTG GCAGCAGCGG GGCCGATCTT 
GCTGGCGCTT CGTTGTGGGG GCCCCCAAAC TATTCTTCTA GCTTGTGGGG AGTCCCAGCT 
GTGGAAGATC CCCATAGGAT GGGCAGCCCT GCTCCCTTAC TACCTGGTGA CCTTCTGGGA 
GGAGGGTCGG ATTCAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424392.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280100.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

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CloneID	OCe291432
Clone ID Related Accession (Same CDS sequence)	XM_022424392.1
Accession Version	XM_022424392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC ATCAGGATTG CTTCTGGCTG TATGACCCAC CTTCCTCTTT CCATCAATCC 
ATGTCCCACC ATTTTCCATT CCATAACAGA AGTCAGCTTC ATGAGAATAC TCTTTGCTTC 
TACTTTAATC TCTTCCAGGG TGCAGTGATC CAGAATCCAG AAGGGCTGTT AGTTGAGAGA 
GAGAAGGAGC AAGAAAGGGA AGAACAGTTA ATGGAAGACA AGAAAAGGAA GAAAGAGGAT 
AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC ACGGAACAAA AAACCAAAGC ACCTGACATT 
CTGAAGCATG CTGTGAACCA CACACTGATT CTGGGATGTC TTAAGTTTGG ACTTCAAGAA 
GCAGTGCCCG AAGTGACGAA ACCAAGTTTA AGCCAACCAA CGGCCGCCAG CCCAATTGGC 
AGCTCTCCAT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CAAGCGCCGC CCGCTACATG CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC 
CAGCAGGACC ACAAAGTGTT ACTAAAACGT GGGCAGCCCC CTCCGCCGTC CTGCATGCTC 
CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC ACAGCACCTG GAGCAAACTC AAACAACGCA 
CAAGTGACAG GAACGCTGCT GCAGAGTGAC AGTGGGACTG CACCAGAGTC AACCCTTGGA 
GGTGCTGCTG CTTCAAATTA TGCAAATTCC ACTTGGGGCC CGGGAGCCTC CTCCAACAAC 
GGCGCCTCCC CCAACCCAAT TCACACCTGG GACAAGGTGA TTGTAGACGG GTCTGACGTG 
GAAGAGTGGC CTTGTATTGC CAGCAAAGAC ACTGAGTCTT CTTCCGAAAA CACCACCGAT 
AACAACAGTG CCTCGAACCC TGGCTCTGAG AAGAGCACTC TGCCAGGAAG CACCACTAGT 
AACAAAGGAA AAGGGAGCCA GTGCCAGTCT GCAAGTTCTG GGAACGAATG TAATCTTGGG 
GTCTGGAAAT CTGACCCTAA GACTAAATCT GTTCAATCTT CCAACTCTAC AACAGAGAGC 
AACAATGGAC TAGGAAATTG GAGGAATGTG AGTGGTCAGG ATAGAATTGG ACCTGGCTCT 
GGCTTCAGCA ACTTTAACCC AAATAGCAAC CCATCTGCCT GGCCAGCGCT GGTCCAAGAA 
GGAAATTCTA GGAAAGGGGC ATTGGAAACG GATACTAGTA ATTCCAGTGC ACAGGTTAGC 
ACAGTAGGTC AGGCATCCAG GGAACAGCAG TCAAAGATGG AAAATGCGGG TGTTAATTTT 
GTTGTCTCTG GCAGAGAACA GGCTCAGATT CATAACACTG ATGGACCAAA AAATGGAAAC 
ACTAACTCCT TGAACTTAAG TTCACCAAAC CCCATGGAGA ATAAGGGAAT GCCCTTTGGA 
ATGGGCTTGG GGAACGCCTC CAGGAGCACT GATGCCCCTT CACAAAGCAC TGGAGATCGA 
AAGACTGGGA GTGTTGGATC TTGGGGTGCA GCTAGGGGGC CTTCTGGAAC TGACACAGCC 
TCTGGACAAA GCAATTCTGG AAACAATGGG AACAATGGAA AAGATAGAGA GGACTCCTGG 
AAAGGAGCTT CTGTTCAGAA ATCATCTGGG TCAAAAAATG ACTCTTGGGA CAATAACAGG 
TCTACGGGTG GGTCCTGGAA CTTTGGCCCT CAGGACTCTA ATGACAACAA ATGGGGTGAA 
GGGAACAAAA TGACATCTGG GGTCTCTCAG GGAGAATGGA AACAGCCGAC TGGGTCTGAT 
GAGTTGAAAA TTGGAGAATG GAGTGGTCCA AACCAACCAA ATTCTAGCAC TGGAGCATGG 
GACAATCAAA AGGGCCACCC CCTCCCTGAA AACCAAGGCA ATGCCCAGGC TCCCTGTTGG 
GGAAGATCTT CCAGCTCCAC AGGAAGTGAA GTTGGAGGTC AAAGCACTGG AAGCAACCAC 
AAAGCAGGAA GTAGTGACAG TCATAATTCT GGCCGTCGGT CATACCGGCC CACACATCCT 
GATTGTCAGG CTGTCTTACA GACTCTTTTG AGCCGAACTG ATTTGGACCC CAGGGTGCTC 
TCAAACACTG GCTGGGGCCA AACTCAAATT AAGCAGGACA CTGTGTGGGA TATTGAAGAG 
GTGCCAAGGC CTGAGGGGAA ATCGGACAAA GGAACTGAGG GGTGGGAGAG CGCTGCCACA 
CAGACCAAGA ACTCAGGGGG CTGGGGAGAT GCACCCAGCC AAAGCAATCA AATGAAGTCT 
GGATGGGGGG AGCTCTCAGC CTCTACAGAG TGGAAAGACC CCAAGAACAC AGGAGGCTGG 
AATGACTATA AGAACAACAA CTCTTCCAAC TGGGGAGGCG GACGACCAGA TGAAAAGACA 
TCTTCCTCTT GGAATGAGAA CTCCAGCAAG GATCAGGGGT GGGGAGGGGG ACGCCAGCCC 
AATCAAGGAT GGACTTCTGG GAAGAATGGT TGGGGAGAGG AAGTTGATCA GGCGAAAAAC 
AGCAGTTGGG AAAGTTCTGC AAGCAAACCT GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT 
GAAATTGGAA CTTGGGGGAA CAGCGGGAAT GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA 
GATTGCAAAA GGTCTCCAGC GTGGACTGAG ACTGGCAGAC AGCCCAATTC CTGGCATAAA 
CAGCACCAAC AGCAGCAGCA GCCACAGCAG CCACCGCCAC CACAACCAGA GGCTTCTGGT 
TCATGGGGAG GCCCCCCACC ACCACCTCCA GGCAACGGAC GACCTTCCAA TTCCAACTGG 
AGCAGTGGGC CACAGCCAGT AACCCCTAAG GACGAGGAAC CCAGTGGTTG GGAAGAGCCA 
TCCCCACAGT CAATTAGTCG GAAAATGGAC ATCGATGATG GCACTTCAGC ATGGGGAGAC 
CCTAACAGCT ATAACTACAA GAATGTGAAT CTGTGGGATA AGAATTCCCA AGGGGGCCCA 
GCACCTCGAG AACCAAACCT GCCTACCCCG ATGACCAGTA AATCAGCATC AGTCTGGAGC 
AAAAGCACAC CACCTGCTCC AGATAATGGT ACTTCAGCTT GGGGTGAGCC AAACGAAAGC 
AGTCCTGGGT GGGGCGAAAT GGATGACACG GGAGCATCGA CCACGGGCTG GGGGAATGCA 
CCCTCCAACG CTCCAAATGC CATGAAACCT AATTCCAAAT CTATGCAAGA CGGCTGGGGG 
GAGAGTGACG GTCCAGTGAC GGGAACTCGC CATCCCAGCT GGGAGGAGGA GGATGATGGA 
GGAGTCTGGA ACACCGCCGG CTCTCAGGGA AGTGCTTCCT CCCACAACTC AGCAAGCTGG 
GGACAAGGAG GAAAGAAGCA AATGAAGTGC TCACTGAAAG GAGGAAACAA TGATTCATGG 
ATGAATCCTC TTGCCAAACA GTTTTCAAAT ATGGGATTGC TGAGTCAAAC TGAAGATAAT 
CCAGGCAGCA AAATGGATTT GTCTGTAGGG AGCCTTCCAG ATAAAAAATT TGACGTGGAC 
AAGCGAGCAA TGAATCTCGG GGATTTTAAT GATATCATGA GGAAGGATCG ATCTGGGTTC 
CGTCCACCTA ATTCCAAAGA CATGGGAACC ACAGATAGTG GGCCTTATTT CGAGAAGGGT 
GGCAATCATG GTTTGTTTGG AAATAGCACA GCACAATCGA GAGGTATGCA CACACCAGTG 
CAGCCACTAA ATTCTTCTCC TAATCTCCGG GCGCAAGTGC CTCCCCAGTT TATTTCCCCC 
CAGGTTTCTG CCTCCATGCT CAAGCAGTTT CCCAACAGTG GCCTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGTTATC TCCTCAACAG ATTGCCATGC TGAGCCAGCT TCCACAAATT 
CCCCAGTTTC AGTTGGCATG TCAGCTTCTC CTGCAGCAGC AGCAACAGCA GCAGTTGCTA 
CAGAACCAGA GGAAGCTTTC TCAAGCTGTG CGCCAGCAGC AAGAGCAGCA GCTGGCTCGA 
ATGGTGAGCG CTCTCCAGCA ACAGCAGCAA CAGCAGCAGC AGCAGCAGCA GAGGCAGCCT 
GGCATGAAGC ACTCACCCTC TCATCCTGTT GGGCCCAAGC CACATCTGGA CAACATGGTA 
CCCAACGCTC TGAATGTGGG GCTCCCAGAC CTTCAAACCA AAGGGCCAAT ACCTGGATAT 
GGTTCTGGCT TCAGCTCTGG CGGCATGGAC TATGGCATGG TTGGTGGGAA GGAGGCTGGA 
ACAGAGTCTC GCTTTAAACA GTGGACCTCC ATGATGGAGG GACTGCCCTC TGTAGCCACA 
CAGGAAGCCA ATATGCACAA AAATGGCGCT ATTGTGGCCC CTGGTAAGAC TCGAGGAGGG 
TCACCGTACA ACCAGTTTGA TATCATCCCG AGTGACACAC TGGGTGGCCA TACGGGTCCT 
GCTGGTGATA GCTGGTTACC TGCCAAATCT CCACCAACAA ATAAAATCGG AAGTAAATCC 
AGCAATGCCA GTTGGCCTCC AGAATTCCAA CCAGGAGTGC CATGGAAAGG TATCCAAAAC 
ATTGACCCTG AATCTGACCC CTATGTCACC CCAGGAAGTG TACTGGGGGG TACAGCCACA 
TCTCCCATTG TAGATACTGA CCACCAACTT CTGCGGGATA ACACCACAGG GTCTAATTCT 
TCCCTCAACA CCTCGCTGCC TTCACCTGGT GCCTGGCCCT ACAGTGCCTC TGACAACTCC 
TTTACCAACG TTCATAGCAC TTCAGCAAAG TTCCCTGATT ACAAATCAAC ATGGTCCCCA 
GATCCCATAG GACACAACCC CACTCATCTC TCCAACAAGA TGTGGAAAAA CCATATTTCC 
TCAAGGAACA CTACACCGCT GCCCCGCCCA CCTCCCGGTC TGACCAACCC CAAACCATCA 
TCTCCCTGGA GCAGCACAGC ACCCCGATCA GTCAGGGGGT GGGGGACACA GGACTCACGG 
CTTGCCTCGG CCTCTACCTG GAGTGATGGT GGTTCAGTTC GTCCCAGTTA CTGGCTGGTT 
CTTCACAATC TCACTCCACA GATTGATGGG TCAACGTTGA GAACCATCTG CATGCAGCAT 
GGCCCACTGC TGACATTCCA TCTGAACCTA ACCCAGGGCA CTGCCCTGAT CCGATACAGC 
ACCAAACAGG AGGCGGCCAA GGCCCAAACT GCACTGCACA TGTGTGTGTT GGGAAACACT 
ACCATCCTGG CTGAGTTTGC CACCGATGAT GAAGTTAGCC GCTTTCTGGC ACAAGCTCAG 
CCCCCTACAC CTGCAGCAAC CCCAAGTGCA CCCGCCGCAG GTTGGCAGTC GCTGGAGACC 
GGCCAGACCC AGTCAGATCC CGTCGGACCT GCTTTGAATC TTTTTGGTGG GTCCACAGGG 
CTCGGGCAAT GGAGCAGCAG TGCTGGTGGC AGCAGCGGGG CCGATCTTGC TGGCGCTTCG 
TTGTGGGGGC CCCCAAACTA TTCTTCTAGC TTGTGGGGAG TCCCAGCTGT GGAAGATCCC 
CATAGGATGG GCAGCCCTGC TCCCTTACTA CCTGGTGACC TTCTGGGAGG AGGGTCGGAT 
TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280100.1
CDS	16..5604
Translation	MDSHQDCFWLYDPPSSFHQSMSHHFPFHNRSQLHENTLCFYFNLFQGAVIQNPEGLLVEREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKAPDILKHAVNHTLILGCLKFGLQEAVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424392.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424392.1

ORF Insert Sequence:

ATGGACTCCC ATCAGGATTG CTTCTGGCTG TATGACCCAC CTTCCTCTTT CCATCAATCC 
ATGTCCCACC ATTTTCCATT CCATAACAGA AGTCAGCTTC ATGAGAATAC TCTTTGCTTC 
TACTTTAATC TCTTCCAGGG TGCAGTGATC CAGAATCCAG AAGGGCTGTT AGTTGAGAGA 
GAGAAGGAGC AAGAAAGGGA AGAACAGTTA ATGGAAGACA AGAAAAGGAA GAAAGAGGAT 
AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC ACGGAACAAA AAACCAAAGC ACCTGACATT 
CTGAAGCATG CTGTGAACCA CACACTGATT CTGGGATGTC TTAAGTTTGG ACTTCAAGAA 
GCAGTGCCCG AAGTGACGAA ACCAAGTTTA AGCCAACCAA CGGCCGCCAG CCCAATTGGC 
AGCTCTCCAT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CAAGCGCCGC CCGCTACATG CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC 
CAGCAGGACC ACAAAGTGTT ACTAAAACGT GGGCAGCCCC CTCCGCCGTC CTGCATGCTC 
CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC ACAGCACCTG GAGCAAACTC AAACAACGCA 
CAAGTGACAG GAACGCTGCT GCAGAGTGAC AGTGGGACTG CACCAGAGTC AACCCTTGGA 
GGTGCTGCTG CTTCAAATTA TGCAAATTCC ACTTGGGGCC CGGGAGCCTC CTCCAACAAC 
GGCGCCTCCC CCAACCCAAT TCACACCTGG GACAAGGTGA TTGTAGACGG GTCTGACGTG 
GAAGAGTGGC CTTGTATTGC CAGCAAAGAC ACTGAGTCTT CTTCCGAAAA CACCACCGAT 
AACAACAGTG CCTCGAACCC TGGCTCTGAG AAGAGCACTC TGCCAGGAAG CACCACTAGT 
AACAAAGGAA AAGGGAGCCA GTGCCAGTCT GCAAGTTCTG GGAACGAATG TAATCTTGGG 
GTCTGGAAAT CTGACCCTAA GACTAAATCT GTTCAATCTT CCAACTCTAC AACAGAGAGC 
AACAATGGAC TAGGAAATTG GAGGAATGTG AGTGGTCAGG ATAGAATTGG ACCTGGCTCT 
GGCTTCAGCA ACTTTAACCC AAATAGCAAC CCATCTGCCT GGCCAGCGCT GGTCCAAGAA 
GGAAATTCTA GGAAAGGGGC ATTGGAAACG GATACTAGTA ATTCCAGTGC ACAGGTTAGC 
ACAGTAGGTC AGGCATCCAG GGAACAGCAG TCAAAGATGG AAAATGCGGG TGTTAATTTT 
GTTGTCTCTG GCAGAGAACA GGCTCAGATT CATAACACTG ATGGACCAAA AAATGGAAAC 
ACTAACTCCT TGAACTTAAG TTCACCAAAC CCCATGGAGA ATAAGGGAAT GCCCTTTGGA 
ATGGGCTTGG GGAACGCCTC CAGGAGCACT GATGCCCCTT CACAAAGCAC TGGAGATCGA 
AAGACTGGGA GTGTTGGATC TTGGGGTGCA GCTAGGGGGC CTTCTGGAAC TGACACAGCC 
TCTGGACAAA GCAATTCTGG AAACAATGGG AACAATGGAA AAGATAGAGA GGACTCCTGG 
AAAGGAGCTT CTGTTCAGAA ATCATCTGGG TCAAAAAATG ACTCTTGGGA CAATAACAGG 
TCTACGGGTG GGTCCTGGAA CTTTGGCCCT CAGGACTCTA ATGACAACAA ATGGGGTGAA 
GGGAACAAAA TGACATCTGG GGTCTCTCAG GGAGAATGGA AACAGCCGAC TGGGTCTGAT 
GAGTTGAAAA TTGGAGAATG GAGTGGTCCA AACCAACCAA ATTCTAGCAC TGGAGCATGG 
GACAATCAAA AGGGCCACCC CCTCCCTGAA AACCAAGGCA ATGCCCAGGC TCCCTGTTGG 
GGAAGATCTT CCAGCTCCAC AGGAAGTGAA GTTGGAGGTC AAAGCACTGG AAGCAACCAC 
AAAGCAGGAA GTAGTGACAG TCATAATTCT GGCCGTCGGT CATACCGGCC CACACATCCT 
GATTGTCAGG CTGTCTTACA GACTCTTTTG AGCCGAACTG ATTTGGACCC CAGGGTGCTC 
TCAAACACTG GCTGGGGCCA AACTCAAATT AAGCAGGACA CTGTGTGGGA TATTGAAGAG 
GTGCCAAGGC CTGAGGGGAA ATCGGACAAA GGAACTGAGG GGTGGGAGAG CGCTGCCACA 
CAGACCAAGA ACTCAGGGGG CTGGGGAGAT GCACCCAGCC AAAGCAATCA AATGAAGTCT 
GGATGGGGGG AGCTCTCAGC CTCTACAGAG TGGAAAGACC CCAAGAACAC AGGAGGCTGG 
AATGACTATA AGAACAACAA CTCTTCCAAC TGGGGAGGCG GACGACCAGA TGAAAAGACA 
TCTTCCTCTT GGAATGAGAA CTCCAGCAAG GATCAGGGGT GGGGAGGGGG ACGCCAGCCC 
AATCAAGGAT GGACTTCTGG GAAGAATGGT TGGGGAGAGG AAGTTGATCA GGCGAAAAAC 
AGCAGTTGGG AAAGTTCTGC AAGCAAACCT GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT 
GAAATTGGAA CTTGGGGGAA CAGCGGGAAT GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA 
GATTGCAAAA GGTCTCCAGC GTGGACTGAG ACTGGCAGAC AGCCCAATTC CTGGCATAAA 
CAGCACCAAC AGCAGCAGCA GCCACAGCAG CCACCGCCAC CACAACCAGA GGCTTCTGGT 
TCATGGGGAG GCCCCCCACC ACCACCTCCA GGCAACGGAC GACCTTCCAA TTCCAACTGG 
AGCAGTGGGC CACAGCCAGT AACCCCTAAG GACGAGGAAC CCAGTGGTTG GGAAGAGCCA 
TCCCCACAGT CAATTAGTCG GAAAATGGAC ATCGATGATG GCACTTCAGC ATGGGGAGAC 
CCTAACAGCT ATAACTACAA GAATGTGAAT CTGTGGGATA AGAATTCCCA AGGGGGCCCA 
GCACCTCGAG AACCAAACCT GCCTACCCCG ATGACCAGTA AATCAGCATC AGTCTGGAGC 
AAAAGCACAC CACCTGCTCC AGATAATGGT ACTTCAGCTT GGGGTGAGCC AAACGAAAGC 
AGTCCTGGGT GGGGCGAAAT GGATGACACG GGAGCATCGA CCACGGGCTG GGGGAATGCA 
CCCTCCAACG CTCCAAATGC CATGAAACCT AATTCCAAAT CTATGCAAGA CGGCTGGGGG 
GAGAGTGACG GTCCAGTGAC GGGAACTCGC CATCCCAGCT GGGAGGAGGA GGATGATGGA 
GGAGTCTGGA ACACCGCCGG CTCTCAGGGA AGTGCTTCCT CCCACAACTC AGCAAGCTGG 
GGACAAGGAG GAAAGAAGCA AATGAAGTGC TCACTGAAAG GAGGAAACAA TGATTCATGG 
ATGAATCCTC TTGCCAAACA GTTTTCAAAT ATGGGATTGC TGAGTCAAAC TGAAGATAAT 
CCAGGCAGCA AAATGGATTT GTCTGTAGGG AGCCTTCCAG ATAAAAAATT TGACGTGGAC 
AAGCGAGCAA TGAATCTCGG GGATTTTAAT GATATCATGA GGAAGGATCG ATCTGGGTTC 
CGTCCACCTA ATTCCAAAGA CATGGGAACC ACAGATAGTG GGCCTTATTT CGAGAAGGGT 
GGCAATCATG GTTTGTTTGG AAATAGCACA GCACAATCGA GAGGTATGCA CACACCAGTG 
CAGCCACTAA ATTCTTCTCC TAATCTCCGG GCGCAAGTGC CTCCCCAGTT TATTTCCCCC 
CAGGTTTCTG CCTCCATGCT CAAGCAGTTT CCCAACAGTG GCCTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGTTATC TCCTCAACAG ATTGCCATGC TGAGCCAGCT TCCACAAATT 
CCCCAGTTTC AGTTGGCATG TCAGCTTCTC CTGCAGCAGC AGCAACAGCA GCAGTTGCTA 
CAGAACCAGA GGAAGCTTTC TCAAGCTGTG CGCCAGCAGC AAGAGCAGCA GCTGGCTCGA 
ATGGTGAGCG CTCTCCAGCA ACAGCAGCAA CAGCAGCAGC AGCAGCAGCA GAGGCAGCCT 
GGCATGAAGC ACTCACCCTC TCATCCTGTT GGGCCCAAGC CACATCTGGA CAACATGGTA 
CCCAACGCTC TGAATGTGGG GCTCCCAGAC CTTCAAACCA AAGGGCCAAT ACCTGGATAT 
GGTTCTGGCT TCAGCTCTGG CGGCATGGAC TATGGCATGG TTGGTGGGAA GGAGGCTGGA 
ACAGAGTCTC GCTTTAAACA GTGGACCTCC ATGATGGAGG GACTGCCCTC TGTAGCCACA 
CAGGAAGCCA ATATGCACAA AAATGGCGCT ATTGTGGCCC CTGGTAAGAC TCGAGGAGGG 
TCACCGTACA ACCAGTTTGA TATCATCCCG AGTGACACAC TGGGTGGCCA TACGGGTCCT 
GCTGGTGATA GCTGGTTACC TGCCAAATCT CCACCAACAA ATAAAATCGG AAGTAAATCC 
AGCAATGCCA GTTGGCCTCC AGAATTCCAA CCAGGAGTGC CATGGAAAGG TATCCAAAAC 
ATTGACCCTG AATCTGACCC CTATGTCACC CCAGGAAGTG TACTGGGGGG TACAGCCACA 
TCTCCCATTG TAGATACTGA CCACCAACTT CTGCGGGATA ACACCACAGG GTCTAATTCT 
TCCCTCAACA CCTCGCTGCC TTCACCTGGT GCCTGGCCCT ACAGTGCCTC TGACAACTCC 
TTTACCAACG TTCATAGCAC TTCAGCAAAG TTCCCTGATT ACAAATCAAC ATGGTCCCCA 
GATCCCATAG GACACAACCC CACTCATCTC TCCAACAAGA TGTGGAAAAA CCATATTTCC 
TCAAGGAACA CTACACCGCT GCCCCGCCCA CCTCCCGGTC TGACCAACCC CAAACCATCA 
TCTCCCTGGA GCAGCACAGC ACCCCGATCA GTCAGGGGGT GGGGGACACA GGACTCACGG 
CTTGCCTCGG CCTCTACCTG GAGTGATGGT GGTTCAGTTC GTCCCAGTTA CTGGCTGGTT 
CTTCACAATC TCACTCCACA GATTGATGGG TCAACGTTGA GAACCATCTG CATGCAGCAT 
GGCCCACTGC TGACATTCCA TCTGAACCTA ACCCAGGGCA CTGCCCTGAT CCGATACAGC 
ACCAAACAGG AGGCGGCCAA GGCCCAAACT GCACTGCACA TGTGTGTGTT GGGAAACACT 
ACCATCCTGG CTGAGTTTGC CACCGATGAT GAAGTTAGCC GCTTTCTGGC ACAAGCTCAG 
CCCCCTACAC CTGCAGCAAC CCCAAGTGCA CCCGCCGCAG GTTGGCAGTC GCTGGAGACC 
GGCCAGACCC AGTCAGATCC CGTCGGACCT GCTTTGAATC TTTTTGGTGG GTCCACAGGG 
CTCGGGCAAT GGAGCAGCAG TGCTGGTGGC AGCAGCGGGG CCGATCTTGC TGGCGCTTCG 
TTGTGGGGGC CCCCAAACTA TTCTTCTAGC TTGTGGGGAG TCCCAGCTGT GGAAGATCCC 
CATAGGATGG GCAGCCCTGC TCCCTTACTA CCTGGTGACC TTCTGGGAGG AGGGTCGGAT 
TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424393.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280101.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.

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CloneID	OCe291433
Clone ID Related Accession (Same CDS sequence)	XM_022424393.1
Accession Version	XM_022424393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGA AGGAGCAAGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGCACCT 
GACATTCTGA AGCATGCTGT GAACCACACA CTGATTCTGG GATGTCTTAA GTTTGGACTT 
CAAGAAGCAG TGCCCGAAGT GACGAAACCA AGTTTAAGCC AACCAACGGC CGCCAGCCCA 
ATTGGCAGCT CTCCATCGCC ACCAGTCAAT GGTGGCAACA ATGCCAAAAG GGTGGCAGTG 
CCGAACGGAC AACCGCCAAG CGCCGCCCGC TACATGCCTC GGGAGGTGCC GCCGCGATTC 
CGTTGCCAGC AGGACCACAA AGTGTTACTA AAACGTGGGC AGCCCCCTCC GCCGTCCTGC 
ATGCTCCTTG GGGGCGGAGC AGGGCCTCCT CCCTCCACAG CACCTGGAGC AAACTCAAAC 
AACGCACAAG TGACAGGAAC GCTGCTGCAG AGTGACAGTG GGACTGCACC AGAGTCAACC 
CTTGGAGGTG CTGCTGCTTC AAATTATGCA AATTCCACTT GGGGCCCGGG AGCCTCCTCC 
AACAACGGCG CCTCCCCCAA CCCAATTCAC ACCTGGGACA AGGTGATTGT AGACGGGTCT 
GACGTGGAAG AGTGGCCTTG TATTGCCAGC AAAGACACTG AGTCTTCTTC CGAAAACACC 
ACCGATAACA ACAGTGCCTC GAACCCTGGC TCTGAGAAGA GCACTCTGCC AGGAAGCACC 
ACTAGTAACA AAGGAAAAGG GAGCCAGTGC CAGTCTGCAA GTTCTGGGAA CGAATGTAAT 
CTTGGGGTCT GGAAATCTGA CCCTAAGACT AAATCTGTTC AATCTTCCAA CTCTACAACA 
GAGAGCAACA ATGGACTAGG AAATTGGAGG AATGTGAGTG GTCAGGATAG AATTGGACCT 
GGCTCTGGCT TCAGCAACTT TAACCCAAAT AGCAACCCAT CTGCCTGGCC AGCGCTGGTC 
CAAGAAGGAA ATTCTAGGAA AGGGGCATTG GAAACGGATA CTAGTAATTC CAGTGCACAG 
GTTAGCACAG TAGGTCAGGC ATCCAGGGAA CAGCAGTCAA AGATGGAAAA TGCGGGTGTT 
AATTTTGTTG TCTCTGGCAG AGAACAGGCT CAGATTCATA ACACTGATGG ACCAAAAAAT 
GGAAACACTA ACTCCTTGAA CTTAAGTTCA CCAAACCCCA TGGAGAATAA GGGAATGCCC 
TTTGGAATGG GCTTGGGGAA CGCCTCCAGG AGCACTGATG CCCCTTCACA AAGCACTGGA 
GATCGAAAGA CTGGGAGTGT TGGATCTTGG GGTGCAGCTA GGGGGCCTTC TGGAACTGAC 
ACAGCCTCTG GACAAAGCAA TTCTGGAAAC AATGGGAACA ATGGAAAAGA TAGAGAGGAC 
TCCTGGAAAG GAGCTTCTGT TCAGAAATCA TCTGGGTCAA AAAATGACTC TTGGGACAAT 
AACAGGTCTA CGGGTGGGTC CTGGAACTTT GGCCCTCAGG ACTCTAATGA CAACAAATGG 
GGTGAAGGGA ACAAAATGAC ATCTGGGGTC TCTCAGGGAG AATGGAAACA GCCGACTGGG 
TCTGATGAGT TGAAAATTGG AGAATGGAGT GGTCCAAACC AACCAAATTC TAGCACTGGA 
GCATGGGACA ATCAAAAGGG CCACCCCCTC CCTGAAAACC AAGGCAATGC CCAGGCTCCC 
TGTTGGGGAA GATCTTCCAG CTCCACAGGA AGTGAAGTTG GAGGTCAAAG CACTGGAAGC 
AACCACAAAG CAGGAAGTAG TGACAGTCAT AATTCTGGCC GTCGGTCATA CCGGCCCACA 
CATCCTGATT GTCAGGCTGT CTTACAGACT CTTTTGAGCC GAACTGATTT GGACCCCAGG 
GTGCTCTCAA ACACTGGCTG GGGCCAAACT CAAATTAAGC AGGACACTGT GTGGGATATT 
GAAGAGGTGC CAAGGCCTGA GGGGAAATCG GACAAAGGAA CTGAGGGGTG GGAGAGCGCT 
GCCACACAGA CCAAGAACTC AGGGGGCTGG GGAGATGCAC CCAGCCAAAG CAATCAAATG 
AAGTCTGGAT GGGGGGAGCT CTCAGCCTCT ACAGAGTGGA AAGACCCCAA GAACACAGGA 
GGCTGGAATG ACTATAAGAA CAACAACTCT TCCAACTGGG GAGGCGGACG ACCAGATGAA 
AAGACATCTT CCTCTTGGAA TGAGAACTCC AGCAAGGATC AGGGGTGGGG AGGGGGACGC 
CAGCCCAATC AAGGATGGAC TTCTGGGAAG AATGGTTGGG GAGAGGAAGT TGATCAGGCG 
AAAAACAGCA GTTGGGAAAG TTCTGCAAGC AAACCTGTGT CTGGATGGGG AGAAGGAGGG 
CAGAATGAAA TTGGAACTTG GGGGAACAGC GGGAATGCAA GCCTAGCTTC CAAAGGTGGG 
TGGGAAGATT GCAAAAGGTC TCCAGCGTGG ACTGAGACTG GCAGACAGCC CAATTCCTGG 
CATAAACAGC ACCAACAGCA GCAGCAGCCA CAGCAGCCAC CGCCACCACA ACCAGAGGCT 
TCTGGTTCAT GGGGAGGCCC CCCACCACCA CCTCCAGGCA ACGGACGACC TTCCAATTCC 
AACTGGAGCA GTGGGCCACA GCCAGTAACC CCTAAGGACG AGGAACCCAG TGGTTGGGAA 
GAGCCATCCC CACAGTCAAT TAGTCGGAAA ATGGACATCG ATGATGGCAC TTCAGCATGG 
GGAGACCCTA ACAGCTATAA CTACAAGAAT GTGAATCTGT GGGATAAGAA TTCCCAAGGG 
GGCCCAGCAC CTCGAGAACC AAACCTGCCT ACCCCGATGA CCAGTAAATC AGCATCAGTC 
TGGAGCAAAA GCACACCACC TGCTCCAGAT AATGGTACTT CAGCTTGGGG TGAGCCAAAC 
GAAAGCAGTC CTGGGTGGGG CGAAATGGAT GACACGGGAG CATCGACCAC GGGCTGGGGG 
AATGCACCCT CCAACGCTCC AAATGCCATG AAACCTAATT CCAAATCTAT GCAAGACGGC 
TGGGGGGAGA GTGACGGTCC AGTGACGGGA ACTCGCCATC CCAGCTGGGA GGAGGAGGAT 
GATGGAGGAG TCTGGAACAC CGCCGGCTCT CAGGGAAGTG CTTCCTCCCA CAACTCAGCA 
AGCTGGGGAC AAGGAGGAAA GAAGCAAATG AAGTGCTCAC TGAAAGGAGG AAACAATGAT 
TCATGGATGA ATCCTCTTGC CAAACAGTTT TCAAATATGG GATTGCTGAG TCAAACTGAA 
GATAATCCAG GCAGCAAAAT GGATTTGTCT GTAGGGAGCC TTCCAGATAA AAAATTTGAC 
GTGGACAAGC GAGCAATGAA TCTCGGGGAT TTTAATGATA TCATGAGGAA GGATCGATCT 
GGGTTCCGTC CACCTAATTC CAAAGACATG GGAACCACAG ATAGTGGGCC TTATTTCGAG 
AAGCTGACTT TGCCTTTCTC CAATCAAGAT GGGTGCCTTG GGGATGAGGC TCCCTGCTCT 
CCCTTCTCCC CTTCTCCCAG CTACAAGCTG TCTCCCTCTG GTTCCACACT ACCCAACGTC 
AGCCTTGGAG CAATCGGCAC AGGGCTCAAC CCCCAAAACT TCGCTGCTAG ACAAGGTGGC 
AATCATGGTT TGTTTGGAAA TAGCACAGCA CAATCGAGAG GTATGCACAC ACCAGTGCAG 
CCACTAAATT CTTCTCCTAA TCTCCGGGCG CAAGTGCCTC CCCAGTTTAT TTCCCCCCAG 
GTTTCTGCCT CCATGCTCAA GCAGTTTCCC AACAGTGGCC TGAATCCAGG TCTTTTCAAT 
GTGGGGCCCC AGTTATCTCC TCAACAGATT GCCATGCTGA GCCAGCTTCC ACAAATTCCC 
CAGTTTCAGT TGGCATGTCA GCTTCTCCTG CAGCAGCAGC AACAGCAGCA GTTGCTACAG 
AACCAGAGGA AGCTTTCTCA AGCTGTGCGC CAGCAGCAAG AGCAGCAGCT GGCTCGAATG 
GTGAGCGCTC TCCAGCAACA GCAGCAACAG CAGCAGCAGC AGCAGCAGAG GCAGCCTGGC 
ATGAAGCACT CACCCTCTCA TCCTGTTGGG CCCAAGCCAC ATCTGGACAA CATGGTACCC 
AACGCTCTGA ATGTGGGGCT CCCAGACCTT CAAACCAAAG GGCCAATACC TGGATATGGT 
TCTGGCTTCA GCTCTGGCGG CATGGACTAT GGCATGGTTG GTGGGAAGGA GGCTGGAACA 
GAGTCTCGCT TTAAACAGTG GACCTCCATG ATGGAGGGAC TGCCCTCTGT AGCCACACAG 
GAAGCCAATA TGCACAAAAA TGGCGCTATT GTGGCCCCTG GTAAGACTCG AGGAGGGTCA 
CCGTACAACC AGTTTGATAT CATCCCGAGT GACACACTGG GTGGCCATAC GGGTCCTGCT 
GGTGATAGCT GGTTACCTGC CAAATCTCCA CCAACAAATA AAATCGGAAG TAAATCCAGC 
AATGCCAGTT GGCCTCCAGA ATTCCAACCA GGAGTGCCAT GGAAAGGTAT CCAAAACATT 
GACCCTGAAT CTGACCCCTA TGTCACCCCA GGAAGTGTAC TGGGGGGTAC AGCCACATCT 
CCCATTGTAG ATACTGACCA CCAACTTCTG CGGGATAACA CCACAGGGTC TAATTCTTCC 
CTCAACACCT CGCTGCCTTC ACCTGGTGCC TGGCCCTACA GTGCCTCTGA CAACTCCTTT 
ACCAACGTTC ATAGCACTTC AGCAAAGTTC CCTGATTACA AATCAACATG GTCCCCAGAT 
CCCATAGGAC ACAACCCCAC TCATCTCTCC AACAAGATGT GGAAAAACCA TATTTCCTCA 
AGGAACACTA CACCGCTGCC CCGCCCACCT CCCGGTCTGA CCAACCCCAA ACCATCATCT 
CCCTGGAGCA GCACAGCACC CCGATCAGTC AGGGGGTGGG GGACACAGGA CTCACGGCTT 
GCCTCGGCCT CTACCTGGAG TGATGGTGGT TCAGTTCGTC CCAGTTACTG GCTGGTTCTT 
CACAATCTCA CTCCACAGAT TGATGGGTCA ACGTTGAGAA CCATCTGCAT GCAGCATGGC 
CCACTGCTGA CATTCCATCT GAACCTAACC CAGGGCACTG CCCTGATCCG ATACAGCACC 
AAACAGGAGG CGGCCAAGGC CCAAACTGCA CTGCACATGT GTGTGTTGGG AAACACTACC 
ATCCTGGCTG AGTTTGCCAC CGATGATGAA GTTAGCCGCT TTCTGGCACA AGCTCAGCCC 
CCTACACCTG CAGCAACCCC AAGTGCACCC GCCGCAGGTT GGCAGTCGCT GGAGACCGGC 
CAGACCCAGT CAGATCCCGT CGGACCTGCT TTGAATCTTT TTGGTGGGTC CACAGGGCTC 
GGGCAATGGA GCAGCAGTGC TGGTGGCAGC AGCGGGGCCG ATCTTGCTGG CGCTTCGTTG 
TGGGGGCCCC CAAACTATTC TTCTAGCTTG TGGGGAGTCC CAGCTGTGGA AGATCCCCAT 
AGGATGGGCA GCCCTGCTCC CTTACTACCT GGTGACCTTC TGGGAGGAGG GTCGGATTCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280101.1
CDS	219..5804
Translation	MREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKAPDILKHAVNHTLILGCLKFGLQEAVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424393.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424393.1

ORF Insert Sequence:

ATGAGAGAGA AGGAGCAAGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGCACCT 
GACATTCTGA AGCATGCTGT GAACCACACA CTGATTCTGG GATGTCTTAA GTTTGGACTT 
CAAGAAGCAG TGCCCGAAGT GACGAAACCA AGTTTAAGCC AACCAACGGC CGCCAGCCCA 
ATTGGCAGCT CTCCATCGCC ACCAGTCAAT GGTGGCAACA ATGCCAAAAG GGTGGCAGTG 
CCGAACGGAC AACCGCCAAG CGCCGCCCGC TACATGCCTC GGGAGGTGCC GCCGCGATTC 
CGTTGCCAGC AGGACCACAA AGTGTTACTA AAACGTGGGC AGCCCCCTCC GCCGTCCTGC 
ATGCTCCTTG GGGGCGGAGC AGGGCCTCCT CCCTCCACAG CACCTGGAGC AAACTCAAAC 
AACGCACAAG TGACAGGAAC GCTGCTGCAG AGTGACAGTG GGACTGCACC AGAGTCAACC 
CTTGGAGGTG CTGCTGCTTC AAATTATGCA AATTCCACTT GGGGCCCGGG AGCCTCCTCC 
AACAACGGCG CCTCCCCCAA CCCAATTCAC ACCTGGGACA AGGTGATTGT AGACGGGTCT 
GACGTGGAAG AGTGGCCTTG TATTGCCAGC AAAGACACTG AGTCTTCTTC CGAAAACACC 
ACCGATAACA ACAGTGCCTC GAACCCTGGC TCTGAGAAGA GCACTCTGCC AGGAAGCACC 
ACTAGTAACA AAGGAAAAGG GAGCCAGTGC CAGTCTGCAA GTTCTGGGAA CGAATGTAAT 
CTTGGGGTCT GGAAATCTGA CCCTAAGACT AAATCTGTTC AATCTTCCAA CTCTACAACA 
GAGAGCAACA ATGGACTAGG AAATTGGAGG AATGTGAGTG GTCAGGATAG AATTGGACCT 
GGCTCTGGCT TCAGCAACTT TAACCCAAAT AGCAACCCAT CTGCCTGGCC AGCGCTGGTC 
CAAGAAGGAA ATTCTAGGAA AGGGGCATTG GAAACGGATA CTAGTAATTC CAGTGCACAG 
GTTAGCACAG TAGGTCAGGC ATCCAGGGAA CAGCAGTCAA AGATGGAAAA TGCGGGTGTT 
AATTTTGTTG TCTCTGGCAG AGAACAGGCT CAGATTCATA ACACTGATGG ACCAAAAAAT 
GGAAACACTA ACTCCTTGAA CTTAAGTTCA CCAAACCCCA TGGAGAATAA GGGAATGCCC 
TTTGGAATGG GCTTGGGGAA CGCCTCCAGG AGCACTGATG CCCCTTCACA AAGCACTGGA 
GATCGAAAGA CTGGGAGTGT TGGATCTTGG GGTGCAGCTA GGGGGCCTTC TGGAACTGAC 
ACAGCCTCTG GACAAAGCAA TTCTGGAAAC AATGGGAACA ATGGAAAAGA TAGAGAGGAC 
TCCTGGAAAG GAGCTTCTGT TCAGAAATCA TCTGGGTCAA AAAATGACTC TTGGGACAAT 
AACAGGTCTA CGGGTGGGTC CTGGAACTTT GGCCCTCAGG ACTCTAATGA CAACAAATGG 
GGTGAAGGGA ACAAAATGAC ATCTGGGGTC TCTCAGGGAG AATGGAAACA GCCGACTGGG 
TCTGATGAGT TGAAAATTGG AGAATGGAGT GGTCCAAACC AACCAAATTC TAGCACTGGA 
GCATGGGACA ATCAAAAGGG CCACCCCCTC CCTGAAAACC AAGGCAATGC CCAGGCTCCC 
TGTTGGGGAA GATCTTCCAG CTCCACAGGA AGTGAAGTTG GAGGTCAAAG CACTGGAAGC 
AACCACAAAG CAGGAAGTAG TGACAGTCAT AATTCTGGCC GTCGGTCATA CCGGCCCACA 
CATCCTGATT GTCAGGCTGT CTTACAGACT CTTTTGAGCC GAACTGATTT GGACCCCAGG 
GTGCTCTCAA ACACTGGCTG GGGCCAAACT CAAATTAAGC AGGACACTGT GTGGGATATT 
GAAGAGGTGC CAAGGCCTGA GGGGAAATCG GACAAAGGAA CTGAGGGGTG GGAGAGCGCT 
GCCACACAGA CCAAGAACTC AGGGGGCTGG GGAGATGCAC CCAGCCAAAG CAATCAAATG 
AAGTCTGGAT GGGGGGAGCT CTCAGCCTCT ACAGAGTGGA AAGACCCCAA GAACACAGGA 
GGCTGGAATG ACTATAAGAA CAACAACTCT TCCAACTGGG GAGGCGGACG ACCAGATGAA 
AAGACATCTT CCTCTTGGAA TGAGAACTCC AGCAAGGATC AGGGGTGGGG AGGGGGACGC 
CAGCCCAATC AAGGATGGAC TTCTGGGAAG AATGGTTGGG GAGAGGAAGT TGATCAGGCG 
AAAAACAGCA GTTGGGAAAG TTCTGCAAGC AAACCTGTGT CTGGATGGGG AGAAGGAGGG 
CAGAATGAAA TTGGAACTTG GGGGAACAGC GGGAATGCAA GCCTAGCTTC CAAAGGTGGG 
TGGGAAGATT GCAAAAGGTC TCCAGCGTGG ACTGAGACTG GCAGACAGCC CAATTCCTGG 
CATAAACAGC ACCAACAGCA GCAGCAGCCA CAGCAGCCAC CGCCACCACA ACCAGAGGCT 
TCTGGTTCAT GGGGAGGCCC CCCACCACCA CCTCCAGGCA ACGGACGACC TTCCAATTCC 
AACTGGAGCA GTGGGCCACA GCCAGTAACC CCTAAGGACG AGGAACCCAG TGGTTGGGAA 
GAGCCATCCC CACAGTCAAT TAGTCGGAAA ATGGACATCG ATGATGGCAC TTCAGCATGG 
GGAGACCCTA ACAGCTATAA CTACAAGAAT GTGAATCTGT GGGATAAGAA TTCCCAAGGG 
GGCCCAGCAC CTCGAGAACC AAACCTGCCT ACCCCGATGA CCAGTAAATC AGCATCAGTC 
TGGAGCAAAA GCACACCACC TGCTCCAGAT AATGGTACTT CAGCTTGGGG TGAGCCAAAC 
GAAAGCAGTC CTGGGTGGGG CGAAATGGAT GACACGGGAG CATCGACCAC GGGCTGGGGG 
AATGCACCCT CCAACGCTCC AAATGCCATG AAACCTAATT CCAAATCTAT GCAAGACGGC 
TGGGGGGAGA GTGACGGTCC AGTGACGGGA ACTCGCCATC CCAGCTGGGA GGAGGAGGAT 
GATGGAGGAG TCTGGAACAC CGCCGGCTCT CAGGGAAGTG CTTCCTCCCA CAACTCAGCA 
AGCTGGGGAC AAGGAGGAAA GAAGCAAATG AAGTGCTCAC TGAAAGGAGG AAACAATGAT 
TCATGGATGA ATCCTCTTGC CAAACAGTTT TCAAATATGG GATTGCTGAG TCAAACTGAA 
GATAATCCAG GCAGCAAAAT GGATTTGTCT GTAGGGAGCC TTCCAGATAA AAAATTTGAC 
GTGGACAAGC GAGCAATGAA TCTCGGGGAT TTTAATGATA TCATGAGGAA GGATCGATCT 
GGGTTCCGTC CACCTAATTC CAAAGACATG GGAACCACAG ATAGTGGGCC TTATTTCGAG 
AAGCTGACTT TGCCTTTCTC CAATCAAGAT GGGTGCCTTG GGGATGAGGC TCCCTGCTCT 
CCCTTCTCCC CTTCTCCCAG CTACAAGCTG TCTCCCTCTG GTTCCACACT ACCCAACGTC 
AGCCTTGGAG CAATCGGCAC AGGGCTCAAC CCCCAAAACT TCGCTGCTAG ACAAGGTGGC 
AATCATGGTT TGTTTGGAAA TAGCACAGCA CAATCGAGAG GTATGCACAC ACCAGTGCAG 
CCACTAAATT CTTCTCCTAA TCTCCGGGCG CAAGTGCCTC CCCAGTTTAT TTCCCCCCAG 
GTTTCTGCCT CCATGCTCAA GCAGTTTCCC AACAGTGGCC TGAATCCAGG TCTTTTCAAT 
GTGGGGCCCC AGTTATCTCC TCAACAGATT GCCATGCTGA GCCAGCTTCC ACAAATTCCC 
CAGTTTCAGT TGGCATGTCA GCTTCTCCTG CAGCAGCAGC AACAGCAGCA GTTGCTACAG 
AACCAGAGGA AGCTTTCTCA AGCTGTGCGC CAGCAGCAAG AGCAGCAGCT GGCTCGAATG 
GTGAGCGCTC TCCAGCAACA GCAGCAACAG CAGCAGCAGC AGCAGCAGAG GCAGCCTGGC 
ATGAAGCACT CACCCTCTCA TCCTGTTGGG CCCAAGCCAC ATCTGGACAA CATGGTACCC 
AACGCTCTGA ATGTGGGGCT CCCAGACCTT CAAACCAAAG GGCCAATACC TGGATATGGT 
TCTGGCTTCA GCTCTGGCGG CATGGACTAT GGCATGGTTG GTGGGAAGGA GGCTGGAACA 
GAGTCTCGCT TTAAACAGTG GACCTCCATG ATGGAGGGAC TGCCCTCTGT AGCCACACAG 
GAAGCCAATA TGCACAAAAA TGGCGCTATT GTGGCCCCTG GTAAGACTCG AGGAGGGTCA 
CCGTACAACC AGTTTGATAT CATCCCGAGT GACACACTGG GTGGCCATAC GGGTCCTGCT 
GGTGATAGCT GGTTACCTGC CAAATCTCCA CCAACAAATA AAATCGGAAG TAAATCCAGC 
AATGCCAGTT GGCCTCCAGA ATTCCAACCA GGAGTGCCAT GGAAAGGTAT CCAAAACATT 
GACCCTGAAT CTGACCCCTA TGTCACCCCA GGAAGTGTAC TGGGGGGTAC AGCCACATCT 
CCCATTGTAG ATACTGACCA CCAACTTCTG CGGGATAACA CCACAGGGTC TAATTCTTCC 
CTCAACACCT CGCTGCCTTC ACCTGGTGCC TGGCCCTACA GTGCCTCTGA CAACTCCTTT 
ACCAACGTTC ATAGCACTTC AGCAAAGTTC CCTGATTACA AATCAACATG GTCCCCAGAT 
CCCATAGGAC ACAACCCCAC TCATCTCTCC AACAAGATGT GGAAAAACCA TATTTCCTCA 
AGGAACACTA CACCGCTGCC CCGCCCACCT CCCGGTCTGA CCAACCCCAA ACCATCATCT 
CCCTGGAGCA GCACAGCACC CCGATCAGTC AGGGGGTGGG GGACACAGGA CTCACGGCTT 
GCCTCGGCCT CTACCTGGAG TGATGGTGGT TCAGTTCGTC CCAGTTACTG GCTGGTTCTT 
CACAATCTCA CTCCACAGAT TGATGGGTCA ACGTTGAGAA CCATCTGCAT GCAGCATGGC 
CCACTGCTGA CATTCCATCT GAACCTAACC CAGGGCACTG CCCTGATCCG ATACAGCACC 
AAACAGGAGG CGGCCAAGGC CCAAACTGCA CTGCACATGT GTGTGTTGGG AAACACTACC 
ATCCTGGCTG AGTTTGCCAC CGATGATGAA GTTAGCCGCT TTCTGGCACA AGCTCAGCCC 
CCTACACCTG CAGCAACCCC AAGTGCACCC GCCGCAGGTT GGCAGTCGCT GGAGACCGGC 
CAGACCCAGT CAGATCCCGT CGGACCTGCT TTGAATCTTT TTGGTGGGTC CACAGGGCTC 
GGGCAATGGA GCAGCAGTGC TGGTGGCAGC AGCGGGGCCG ATCTTGCTGG CGCTTCGTTG 
TGGGGGCCCC CAAACTATTC TTCTAGCTTG TGGGGAGTCC CAGCTGTGGA AGATCCCCAT 
AGGATGGGCA GCCCTGCTCC CTTACTACCT GGTGACCTTC TGGGAGGAGG GTCGGATTCA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424394.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280102.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X10, mRNA.

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CloneID	OCe291434
Clone ID Related Accession (Same CDS sequence)	XM_022424394.1
Accession Version	XM_022424394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC 
ACGGAACAAA AAACCAAAGC ACCTGACATT CTGAAGCATG CTGTGAACCA CACACTGATT 
CTGGGATGTC TTAAGTTTGG ACTTCAAGAA GCAGTGCCCG AAGTGACGAA ACCAAGTTTA 
AGCCAACCAA CGGCCGCCAG CCCAATTGGC AGCTCTCCAT CGCCACCAGT CAATGGTGGC 
AACAATGCCA AAAGGGTGGC AGTGCCGAAC GGACAACCGC CAAGCGCCGC CCGCTACATG 
CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC CAGCAGGACC ACAAAGTGTT ACTAAAACGT 
GGGCAGCCCC CTCCGCCGTC CTGCATGCTC CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC 
ACAGCACCTG GAGCAAACTC AAACAACGCA CAAGTGACAG GAACGCTGCT GCAGAGTGAC 
AGTGGGACTG CACCAGAGTC AACCCTTGGA GGTGCTGCTG CTTCAAATTA TGCAAATTCC 
ACTTGGGGCC CGGGAGCCTC CTCCAACAAC GGCGCCTCCC CCAACCCAAT TCACACCTGG 
GACAAGGTGA TTGTAGACGG GTCTGACGTG GAAGAGTGGC CTTGTATTGC CAGCAAAGAC 
ACTGAGTCTT CTTCCGAAAA CACCACCGAT AACAACAGTG CCTCGAACCC TGGCTCTGAG 
AAGAGCACTC TGCCAGGAAG CACCACTAGT AACAAAGGAA AAGGGAGCCA GTGCCAGTCT 
GCAAGTTCTG GGAACGAATG TAATCTTGGG GTCTGGAAAT CTGACCCTAA GACTAAATCT 
GTTCAATCTT CCAACTCTAC AACAGAGAGC AACAATGGAC TAGGAAATTG GAGGAATGTG 
AGTGGTCAGG ATAGAATTGG ACCTGGCTCT GGCTTCAGCA ACTTTAACCC AAATAGCAAC 
CCATCTGCCT GGCCAGCGCT GGTCCAAGAA GGAAATTCTA GGAAAGGGGC ATTGGAAACG 
GATACTAGTA ATTCCAGTGC ACAGGTTAGC ACAGTAGGTC AGGCATCCAG GGAACAGCAG 
TCAAAGATGG AAAATGCGGG TGTTAATTTT GTTGTCTCTG GCAGAGAACA GGCTCAGATT 
CATAACACTG ATGGACCAAA AAATGGAAAC ACTAACTCCT TGAACTTAAG TTCACCAAAC 
CCCATGGAGA ATAAGGGAAT GCCCTTTGGA ATGGGCTTGG GGAACGCCTC CAGGAGCACT 
GATGCCCCTT CACAAAGCAC TGGAGATCGA AAGACTGGGA GTGTTGGATC TTGGGGTGCA 
GCTAGGGGGC CTTCTGGAAC TGACACAGCC TCTGGACAAA GCAATTCTGG AAACAATGGG 
AACAATGGAA AAGATAGAGA GGACTCCTGG AAAGGAGCTT CTGTTCAGAA ATCATCTGGG 
TCAAAAAATG ACTCTTGGGA CAATAACAGG TCTACGGGTG GGTCCTGGAA CTTTGGCCCT 
CAGGACTCTA ATGACAACAA ATGGGGTGAA GGGAACAAAA TGACATCTGG GGTCTCTCAG 
GGAGAATGGA AACAGCCGAC TGGGTCTGAT GAGTTGAAAA TTGGAGAATG GAGTGGTCCA 
AACCAACCAA ATTCTAGCAC TGGAGCATGG GACAATCAAA AGGGCCACCC CCTCCCTGAA 
AACCAAGGCA ATGCCCAGGC TCCCTGTTGG GGAAGATCTT CCAGCTCCAC AGGAAGTGAA 
GTTGGAGGTC AAAGCACTGG AAGCAACCAC AAAGCAGGAA GTAGTGACAG TCATAATTCT 
GGCCGTCGGT CATACCGGCC CACACATCCT GATTGTCAGG CTGTCTTACA GACTCTTTTG 
AGCCGAACTG ATTTGGACCC CAGGGTGCTC TCAAACACTG GCTGGGGCCA AACTCAAATT 
AAGCAGGACA CTGTGTGGGA TATTGAAGAG GTGCCAAGGC CTGAGGGGAA ATCGGACAAA 
GGAACTGAGG GGTGGGAGAG CGCTGCCACA CAGACCAAGA ACTCAGGGGG CTGGGGAGAT 
GCACCCAGCC AAAGCAATCA AATGAAGTCT GGATGGGGGG AGCTCTCAGC CTCTACAGAG 
TGGAAAGACC CCAAGAACAC AGGAGGCTGG AATGACTATA AGAACAACAA CTCTTCCAAC 
TGGGGAGGCG GACGACCAGA TGAAAAGACA TCTTCCTCTT GGAATGAGAA CTCCAGCAAG 
GATCAGGGGT GGGGAGGGGG ACGCCAGCCC AATCAAGGAT GGACTTCTGG GAAGAATGGT 
TGGGGAGAGG AAGTTGATCA GGCGAAAAAC AGCAGTTGGG AAAGTTCTGC AAGCAAACCT 
GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT GAAATTGGAA CTTGGGGGAA CAGCGGGAAT 
GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA GATTGCAAAA GGTCTCCAGC GTGGACTGAG 
ACTGGCAGAC AGCCCAATTC CTGGCATAAA CAGCACCAAC AGCAGCAGCA GCCACAGCAG 
CCACCGCCAC CACAACCAGA GGCTTCTGGT TCATGGGGAG GCCCCCCACC ACCACCTCCA 
GGCAACGGAC GACCTTCCAA TTCCAACTGG AGCAGTGGGC CACAGCCAGT AACCCCTAAG 
GACGAGGAAC CCAGTGGTTG GGAAGAGCCA TCCCCACAGT CAATTAGTCG GAAAATGGAC 
ATCGATGATG GCACTTCAGC ATGGGGAGAC CCTAACAGCT ATAACTACAA GAATGTGAAT 
CTGTGGGATA AGAATTCCCA AGGGGGCCCA GCACCTCGAG AACCAAACCT GCCTACCCCG 
ATGACCAGTA AATCAGCATC AGTCTGGAGC AAAAGCACAC CACCTGCTCC AGATAATGGT 
ACTTCAGCTT GGGGTGAGCC AAACGAAAGC AGTCCTGGGT GGGGCGAAAT GGATGACACG 
GGAGCATCGA CCACGGGCTG GGGGAATGCA CCCTCCAACG CTCCAAATGC CATGAAACCT 
AATTCCAAAT CTATGCAAGA CGGCTGGGGG GAGAGTGACG GTCCAGTGAC GGGAACTCGC 
CATCCCAGCT GGGAGGAGGA GGATGATGGA GGAGTCTGGA ACACCGCCGG CTCTCAGGGA 
AGTGCTTCCT CCCACAACTC AGCAAGCTGG GGACAAGGAG GAAAGAAGCA AATGAAGTGC 
TCACTGAAAG GAGGAAACAA TGATTCATGG ATGAATCCTC TTGCCAAACA GTTTTCAAAT 
ATGGGATTGC TGAGTCAAAC TGAAGATAAT CCAGGCAGCA AAATGGATTT GTCTGTAGGG 
AGCCTTCCAG ATAAAAAATT TGACGTGGAC AAGCGAGCAA TGAATCTCGG GGATTTTAAT 
GATATCATGA GGAAGGATCG ATCTGGGTTC CGTCCACCTA ATTCCAAAGA CATGGGAACC 
ACAGATAGTG GGCCTTATTT CGAGAAGCTG ACTTTGCCTT TCTCCAATCA AGATGGGTGC 
CTTGGGGATG AGGCTCCCTG CTCTCCCTTC TCCCCTTCTC CCAGCTACAA GCTGTCTCCC 
TCTGGTTCCA CACTACCCAA CGTCAGCCTT GGAGCAATCG GCACAGGGCT CAACCCCCAA 
AACTTCGCTG CTAGACAAGG TGGCAATCAT GGTTTGTTTG GAAATAGCAC AGCACAATCG 
AGAGGTATGC ACACACCAGT GCAGCCACTA AATTCTTCTC CTAATCTCCG GGCGCAAGTG 
CCTCCCCAGT TTATTTCCCC CCAGGTTTCT GCCTCCATGC TCAAGCAGTT TCCCAACAGT 
GGCCTGAATC CAGGTCTTTT CAATGTGGGG CCCCAGTTAT CTCCTCAACA GATTGCCATG 
CTGAGCCAGC TTCCACAAAT TCCCCAGTTT CAGTTGGCAT GTCAGCTTCT CCTGCAGCAG 
CAGCAACAGC AGCAGTTGCT ACAGAACCAG AGGAAGCTTT CTCAAGCTGT GCGCCAGCAG 
CAAGAGCAGC AGCTGGCTCG AATGGTGAGC GCTCTCCAGC AACAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGAGGCAGCC TGGCATGAAG CACTCACCCT CTCATCCTGT TGGGCCCAAG 
CCACATCTGG ACAACATGGT ACCCAACGCT CTGAATGTGG GGCTCCCAGA CCTTCAAACC 
AAAGGGCCAA TACCTGGATA TGGTTCTGGC TTCAGCTCTG GCGGCATGGA CTATGGCATG 
GTTGGTGGGA AGGAGGCTGG AACAGAGTCT CGCTTTAAAC AGTGGACCTC CATGATGGAG 
GGACTGCCCT CTGTAGCCAC ACAGGAAGCC AATATGCACA AAAATGGCGC TATTGTGGCC 
CCTGGTAAGA CTCGAGGAGG GTCACCGTAC AACCAGTTTG ATATCATCCC GAGTGACACA 
CTGGGTGGCC ATACGGGTCC TGCTGGTGAT AGCTGGTTAC CTGCCAAATC TCCACCAACA 
AATAAAATCG GAAGTAAATC CAGCAATGCC AGTTGGCCTC CAGAATTCCA ACCAGGAGTG 
CCATGGAAAG GTATCCAAAA CATTGACCCT GAATCTGACC CCTATGTCAC CCCAGGAAGT 
GTACTGGGGG GTACAGCCAC ATCTCCCATT GTAGATACTG ACCACCAACT TCTGCGGGAT 
AACACCACAG GGTCTAATTC TTCCCTCAAC ACCTCGCTGC CTTCACCTGG TGCCTGGCCC 
TACAGTGCCT CTGACAACTC CTTTACCAAC GTTCATAGCA CTTCAGCAAA GTTCCCTGAT 
TACAAATCAA CATGGTCCCC AGATCCCATA GGACACAACC CCACTCATCT CTCCAACAAG 
ATGTGGAAAA ACCATATTTC CTCAAGGAAC ACTACACCGC TGCCCCGCCC ACCTCCCGGT 
CTGACCAACC CCAAACCATC ATCTCCCTGG AGCAGCACAG CACCCCGATC AGTCAGGGGG 
TGGGGGACAC AGGACTCACG GCTTGCCTCG GCCTCTACCT GGAGTGATGG TGGTTCAGTT 
CGTCCCAGTT ACTGGCTGGT TCTTCACAAT CTCACTCCAC AGATTGATGG GTCAACGTTG 
AGAACCATCT GCATGCAGCA TGGCCCACTG CTGACATTCC ATCTGAACCT AACCCAGGGC 
ACTGCCCTGA TCCGATACAG CACCAAACAG GAGGCGGCCA AGGCCCAAAC TGCACTGCAC 
ATGTGTGTGT TGGGAAACAC TACCATCCTG GCTGAGTTTG CCACCGATGA TGAAGTTAGC 
CGCTTTCTGG CACAAGCTCA GCCCCCTACA CCTGCAGCAA CCCCAAGTGC ACCCGCCGCA 
GGTTGGCAGT CGCTGGAGAC CGGCCAGACC CAGTCAGATC CCGTCGGACC TGCTTTGAAT 
CTTTTTGGTG GGTCCACAGG GCTCGGGCAA TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG 
GCCGATCTTG CTGGCGCTTC GTTGTGGGGG CCCCCAAACT ATTCTTCTAG CTTGTGGGGA 
GTCCCAGCTG TGGAAGATCC CCATAGGATG GGCAGCCCTG CTCCCTTACT ACCTGGTGAC 
CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280102.1
CDS	251..5800
Translation	MEDKKRKKEDKKKKEATQKVTEQKTKAPDILKHAVNHTLILGCLKFGLQEAVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424394.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424394.1

ORF Insert Sequence:

ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC 
ACGGAACAAA AAACCAAAGC ACCTGACATT CTGAAGCATG CTGTGAACCA CACACTGATT 
CTGGGATGTC TTAAGTTTGG ACTTCAAGAA GCAGTGCCCG AAGTGACGAA ACCAAGTTTA 
AGCCAACCAA CGGCCGCCAG CCCAATTGGC AGCTCTCCAT CGCCACCAGT CAATGGTGGC 
AACAATGCCA AAAGGGTGGC AGTGCCGAAC GGACAACCGC CAAGCGCCGC CCGCTACATG 
CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC CAGCAGGACC ACAAAGTGTT ACTAAAACGT 
GGGCAGCCCC CTCCGCCGTC CTGCATGCTC CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC 
ACAGCACCTG GAGCAAACTC AAACAACGCA CAAGTGACAG GAACGCTGCT GCAGAGTGAC 
AGTGGGACTG CACCAGAGTC AACCCTTGGA GGTGCTGCTG CTTCAAATTA TGCAAATTCC 
ACTTGGGGCC CGGGAGCCTC CTCCAACAAC GGCGCCTCCC CCAACCCAAT TCACACCTGG 
GACAAGGTGA TTGTAGACGG GTCTGACGTG GAAGAGTGGC CTTGTATTGC CAGCAAAGAC 
ACTGAGTCTT CTTCCGAAAA CACCACCGAT AACAACAGTG CCTCGAACCC TGGCTCTGAG 
AAGAGCACTC TGCCAGGAAG CACCACTAGT AACAAAGGAA AAGGGAGCCA GTGCCAGTCT 
GCAAGTTCTG GGAACGAATG TAATCTTGGG GTCTGGAAAT CTGACCCTAA GACTAAATCT 
GTTCAATCTT CCAACTCTAC AACAGAGAGC AACAATGGAC TAGGAAATTG GAGGAATGTG 
AGTGGTCAGG ATAGAATTGG ACCTGGCTCT GGCTTCAGCA ACTTTAACCC AAATAGCAAC 
CCATCTGCCT GGCCAGCGCT GGTCCAAGAA GGAAATTCTA GGAAAGGGGC ATTGGAAACG 
GATACTAGTA ATTCCAGTGC ACAGGTTAGC ACAGTAGGTC AGGCATCCAG GGAACAGCAG 
TCAAAGATGG AAAATGCGGG TGTTAATTTT GTTGTCTCTG GCAGAGAACA GGCTCAGATT 
CATAACACTG ATGGACCAAA AAATGGAAAC ACTAACTCCT TGAACTTAAG TTCACCAAAC 
CCCATGGAGA ATAAGGGAAT GCCCTTTGGA ATGGGCTTGG GGAACGCCTC CAGGAGCACT 
GATGCCCCTT CACAAAGCAC TGGAGATCGA AAGACTGGGA GTGTTGGATC TTGGGGTGCA 
GCTAGGGGGC CTTCTGGAAC TGACACAGCC TCTGGACAAA GCAATTCTGG AAACAATGGG 
AACAATGGAA AAGATAGAGA GGACTCCTGG AAAGGAGCTT CTGTTCAGAA ATCATCTGGG 
TCAAAAAATG ACTCTTGGGA CAATAACAGG TCTACGGGTG GGTCCTGGAA CTTTGGCCCT 
CAGGACTCTA ATGACAACAA ATGGGGTGAA GGGAACAAAA TGACATCTGG GGTCTCTCAG 
GGAGAATGGA AACAGCCGAC TGGGTCTGAT GAGTTGAAAA TTGGAGAATG GAGTGGTCCA 
AACCAACCAA ATTCTAGCAC TGGAGCATGG GACAATCAAA AGGGCCACCC CCTCCCTGAA 
AACCAAGGCA ATGCCCAGGC TCCCTGTTGG GGAAGATCTT CCAGCTCCAC AGGAAGTGAA 
GTTGGAGGTC AAAGCACTGG AAGCAACCAC AAAGCAGGAA GTAGTGACAG TCATAATTCT 
GGCCGTCGGT CATACCGGCC CACACATCCT GATTGTCAGG CTGTCTTACA GACTCTTTTG 
AGCCGAACTG ATTTGGACCC CAGGGTGCTC TCAAACACTG GCTGGGGCCA AACTCAAATT 
AAGCAGGACA CTGTGTGGGA TATTGAAGAG GTGCCAAGGC CTGAGGGGAA ATCGGACAAA 
GGAACTGAGG GGTGGGAGAG CGCTGCCACA CAGACCAAGA ACTCAGGGGG CTGGGGAGAT 
GCACCCAGCC AAAGCAATCA AATGAAGTCT GGATGGGGGG AGCTCTCAGC CTCTACAGAG 
TGGAAAGACC CCAAGAACAC AGGAGGCTGG AATGACTATA AGAACAACAA CTCTTCCAAC 
TGGGGAGGCG GACGACCAGA TGAAAAGACA TCTTCCTCTT GGAATGAGAA CTCCAGCAAG 
GATCAGGGGT GGGGAGGGGG ACGCCAGCCC AATCAAGGAT GGACTTCTGG GAAGAATGGT 
TGGGGAGAGG AAGTTGATCA GGCGAAAAAC AGCAGTTGGG AAAGTTCTGC AAGCAAACCT 
GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT GAAATTGGAA CTTGGGGGAA CAGCGGGAAT 
GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA GATTGCAAAA GGTCTCCAGC GTGGACTGAG 
ACTGGCAGAC AGCCCAATTC CTGGCATAAA CAGCACCAAC AGCAGCAGCA GCCACAGCAG 
CCACCGCCAC CACAACCAGA GGCTTCTGGT TCATGGGGAG GCCCCCCACC ACCACCTCCA 
GGCAACGGAC GACCTTCCAA TTCCAACTGG AGCAGTGGGC CACAGCCAGT AACCCCTAAG 
GACGAGGAAC CCAGTGGTTG GGAAGAGCCA TCCCCACAGT CAATTAGTCG GAAAATGGAC 
ATCGATGATG GCACTTCAGC ATGGGGAGAC CCTAACAGCT ATAACTACAA GAATGTGAAT 
CTGTGGGATA AGAATTCCCA AGGGGGCCCA GCACCTCGAG AACCAAACCT GCCTACCCCG 
ATGACCAGTA AATCAGCATC AGTCTGGAGC AAAAGCACAC CACCTGCTCC AGATAATGGT 
ACTTCAGCTT GGGGTGAGCC AAACGAAAGC AGTCCTGGGT GGGGCGAAAT GGATGACACG 
GGAGCATCGA CCACGGGCTG GGGGAATGCA CCCTCCAACG CTCCAAATGC CATGAAACCT 
AATTCCAAAT CTATGCAAGA CGGCTGGGGG GAGAGTGACG GTCCAGTGAC GGGAACTCGC 
CATCCCAGCT GGGAGGAGGA GGATGATGGA GGAGTCTGGA ACACCGCCGG CTCTCAGGGA 
AGTGCTTCCT CCCACAACTC AGCAAGCTGG GGACAAGGAG GAAAGAAGCA AATGAAGTGC 
TCACTGAAAG GAGGAAACAA TGATTCATGG ATGAATCCTC TTGCCAAACA GTTTTCAAAT 
ATGGGATTGC TGAGTCAAAC TGAAGATAAT CCAGGCAGCA AAATGGATTT GTCTGTAGGG 
AGCCTTCCAG ATAAAAAATT TGACGTGGAC AAGCGAGCAA TGAATCTCGG GGATTTTAAT 
GATATCATGA GGAAGGATCG ATCTGGGTTC CGTCCACCTA ATTCCAAAGA CATGGGAACC 
ACAGATAGTG GGCCTTATTT CGAGAAGCTG ACTTTGCCTT TCTCCAATCA AGATGGGTGC 
CTTGGGGATG AGGCTCCCTG CTCTCCCTTC TCCCCTTCTC CCAGCTACAA GCTGTCTCCC 
TCTGGTTCCA CACTACCCAA CGTCAGCCTT GGAGCAATCG GCACAGGGCT CAACCCCCAA 
AACTTCGCTG CTAGACAAGG TGGCAATCAT GGTTTGTTTG GAAATAGCAC AGCACAATCG 
AGAGGTATGC ACACACCAGT GCAGCCACTA AATTCTTCTC CTAATCTCCG GGCGCAAGTG 
CCTCCCCAGT TTATTTCCCC CCAGGTTTCT GCCTCCATGC TCAAGCAGTT TCCCAACAGT 
GGCCTGAATC CAGGTCTTTT CAATGTGGGG CCCCAGTTAT CTCCTCAACA GATTGCCATG 
CTGAGCCAGC TTCCACAAAT TCCCCAGTTT CAGTTGGCAT GTCAGCTTCT CCTGCAGCAG 
CAGCAACAGC AGCAGTTGCT ACAGAACCAG AGGAAGCTTT CTCAAGCTGT GCGCCAGCAG 
CAAGAGCAGC AGCTGGCTCG AATGGTGAGC GCTCTCCAGC AACAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGAGGCAGCC TGGCATGAAG CACTCACCCT CTCATCCTGT TGGGCCCAAG 
CCACATCTGG ACAACATGGT ACCCAACGCT CTGAATGTGG GGCTCCCAGA CCTTCAAACC 
AAAGGGCCAA TACCTGGATA TGGTTCTGGC TTCAGCTCTG GCGGCATGGA CTATGGCATG 
GTTGGTGGGA AGGAGGCTGG AACAGAGTCT CGCTTTAAAC AGTGGACCTC CATGATGGAG 
GGACTGCCCT CTGTAGCCAC ACAGGAAGCC AATATGCACA AAAATGGCGC TATTGTGGCC 
CCTGGTAAGA CTCGAGGAGG GTCACCGTAC AACCAGTTTG ATATCATCCC GAGTGACACA 
CTGGGTGGCC ATACGGGTCC TGCTGGTGAT AGCTGGTTAC CTGCCAAATC TCCACCAACA 
AATAAAATCG GAAGTAAATC CAGCAATGCC AGTTGGCCTC CAGAATTCCA ACCAGGAGTG 
CCATGGAAAG GTATCCAAAA CATTGACCCT GAATCTGACC CCTATGTCAC CCCAGGAAGT 
GTACTGGGGG GTACAGCCAC ATCTCCCATT GTAGATACTG ACCACCAACT TCTGCGGGAT 
AACACCACAG GGTCTAATTC TTCCCTCAAC ACCTCGCTGC CTTCACCTGG TGCCTGGCCC 
TACAGTGCCT CTGACAACTC CTTTACCAAC GTTCATAGCA CTTCAGCAAA GTTCCCTGAT 
TACAAATCAA CATGGTCCCC AGATCCCATA GGACACAACC CCACTCATCT CTCCAACAAG 
ATGTGGAAAA ACCATATTTC CTCAAGGAAC ACTACACCGC TGCCCCGCCC ACCTCCCGGT 
CTGACCAACC CCAAACCATC ATCTCCCTGG AGCAGCACAG CACCCCGATC AGTCAGGGGG 
TGGGGGACAC AGGACTCACG GCTTGCCTCG GCCTCTACCT GGAGTGATGG TGGTTCAGTT 
CGTCCCAGTT ACTGGCTGGT TCTTCACAAT CTCACTCCAC AGATTGATGG GTCAACGTTG 
AGAACCATCT GCATGCAGCA TGGCCCACTG CTGACATTCC ATCTGAACCT AACCCAGGGC 
ACTGCCCTGA TCCGATACAG CACCAAACAG GAGGCGGCCA AGGCCCAAAC TGCACTGCAC 
ATGTGTGTGT TGGGAAACAC TACCATCCTG GCTGAGTTTG CCACCGATGA TGAAGTTAGC 
CGCTTTCTGG CACAAGCTCA GCCCCCTACA CCTGCAGCAA CCCCAAGTGC ACCCGCCGCA 
GGTTGGCAGT CGCTGGAGAC CGGCCAGACC CAGTCAGATC CCGTCGGACC TGCTTTGAAT 
CTTTTTGGTG GGTCCACAGG GCTCGGGCAA TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG 
GCCGATCTTG CTGGCGCTTC GTTGTGGGGG CCCCCAAACT ATTCTTCTAG CTTGTGGGGA 
GTCCCAGCTG TGGAAGATCC CCATAGGATG GGCAGCCCTG CTCCCTTACT ACCTGGTGAC 
CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424395.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280103.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X11, mRNA.

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CloneID	OCe291435
Clone ID Related Accession (Same CDS sequence)	XM_022424395.1
Accession Version	XM_022424395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC ATCAGGATTG CTTCTGGCTG TATGACCCAC CTTCCTCTTT CCATCAATCC 
ATGTCCCACC ATTTTCCATT CCATAACAGA AGTCAGCTTC ATGAGAATAC TCTTTGCTTC 
TACTTTAATC TCTTCCAGGG TGCAGTGATC CAGAATCCAG AAGGGCTGTT AGTTGAGAGA 
GAGAAGGAGC AAGAAAGGGA AGAACAGTTA ATGGAAGACA AGAAAAGGAA GAAAGAGGAT 
AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC ACGGAACAAA AAACCAAAGC ACCTGACATT 
CTGAAGCATG CTGTGAACCA CACACTGATT CTGGGATGTC TTAAGTTTGG ACTTCAAGAA 
GCAGTGCCCG AAGTGACGAA ACCAAGTTTA AGCCAACCAA CGGCCGCCAG CCCAATTGGC 
AGCTCTCCAT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CAAGCGCCGC CCGCTACATG CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC 
CAGCAGGACC ACAAAGTGTT ACTAAAACGT GGGCAGCCCC CTCCGCCGTC CTGCATGCTC 
CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC ACAGCACCTG GAGCAAACTC AAACAACGCA 
CAAGTGACAG GAACGCTGCT GCAGAGTGAC AGTGGGACTG CACCAGAGTC AACCCTTGGA 
GGTGCTGCTG CTTCAAATTA TGCAAATTCC ACTTGGGGCC CGGGAGCCTC CTCCAACAAC 
GGCGCCTCCC CCAACCCAAT TCACACCTGG GACAAGGTGA TTGTAGACGG GTCTGACGTG 
GAAGAGTGGC CTTGTATTGC CAGCAAAGAC ACTGAGTCTT CTTCCGAAAA CACCACCGAT 
AACAACAGTG CCTCGAACCC TGGCTCTGAG AAGAGCACTC TGCCAGGAAG CACCACTAGT 
AACAAAGGAA AAGGGAGCCA GTGCCAGTCT GCAAGTTCTG GGAACGAATG TAATCTTGGG 
GTCTGGAAAT CTGACCCTAA GACTAAATCT GTTCAATCTT CCAACTCTAC AACAGAGAGC 
AACAATGGAC TAGGAAATTG GAGGAATGTG AGTGGTCAGG ATAGAATTGG ACCTGGCTCT 
GGCTTCAGCA ACTTTAACCC AAATAGCAAC CCATCTGCCT GGCCAGCGCT GGTCCAAGAA 
GGAAATTCTA GGAAAGGGGC ATTGGAAACG GATACTAGTA ATTCCAGTGC ACAGGTTAGC 
ACAGTAGGTC AGGCATCCAG GGAACAGCAG TCAAAGATGG AAAATGCGGG TGTTAATTTT 
GTTGTCTCTG GCAGAGAACA GGCTCAGATT CATAACACTG ATGGACCAAA AAATGGAAAC 
ACTAACTCCT TGAACTTAAG TTCACCAAAC CCCATGGAGA ATAAGGGAAT GCCCTTTGGA 
ATGGGCTTGG GGAACGCCTC CAGGAGCACT GATGCCCCTT CACAAAGCAC TGGAGATCGA 
AAGACTGGGA GTGTTGGATC TTGGGGTGCA GCTAGGGGGC CTTCTGGAAC TGACACAGCC 
TCTGGACAAA GCAATTCTGG AAACAATGGG AACAATGGAA AAGATAGAGA GGACTCCTGG 
AAAGGAGCTT CTGTTCAGAA ATCATCTGGG TCAAAAAATG ACTCTTGGGA CAATAACAGG 
TCTACGGGTG GGTCCTGGAA CTTTGGCCCT CAGGACTCTA ATGACAACAA ATGGGGTGAA 
GGGAACAAAA TGACATCTGG GGTCTCTCAG GGAGAATGGA AACAGCCGAC TGGGTCTGAT 
GAGTTGAAAA TTGGAGAATG GAGTGGTCCA AACCAACCAA ATTCTAGCAC TGGAGCATGG 
GACAATCAAA AGGGCCACCC CCTCCCTGAA AACCAAGGCA ATGCCCAGGC TCCCTGTTGG 
GGAAGATCTT CCAGCTCCAC AGGAAGTGAA GTTGGAGGTC AAAGCACTGG AAGCAACCAC 
AAAGCAGGAA GTAGTGACAG TCATAATTCT GGCCGTCGGT CATACCGGCC CACACATCCT 
GATTGTCAGG CTGTCTTACA GACTCTTTTG AGCCGAACTG ATTTGGACCC CAGGGTGCTC 
TCAAACACTG GCTGGGGCCA AACTCAAATT AAGCAGGACA CTGTGTGGGA TATTGAAGAG 
GTGCCAAGGC CTGAGGGGAA ATCGGACAAA GGAACTGAGG GGTGGGAGAG CGCTGCCACA 
CAGACCAAGA ACTCAGGGGG CTGGGGAGAT GCACCCAGCC AAAGCAATCA AATGAAGTCT 
GGATGGGGGG AGCTCTCAGC CTCTACAGAG TGGAAAGACC CCAAGAACAC AGGAGGCTGG 
AATGACTATA AGAACAACAA CTCTTCCAAC TGGGGAGGCG GACGACCAGA TGAAAAGACA 
TCTTCCTCTT GGAATGAGAA CTCCAGCAAG GATCAGGGGT GGGGAGGGGG ACGCCAGCCC 
AATCAAGGAT GGACTTCTGG GAAGAATGGT TGGGGAGAGG AAGTTGATCA GGCGAAAAAC 
AGCAGTTGGG AAAGTTCTGC AAGCAAACCT GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT 
GAAATTGGAA CTTGGGGGAA CAGCGGGAAT GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA 
GATTGCAAAA GGTCTCCAGC GTGGACTGAG ACTGGCAGAC AGCCCAATTC CTGGCATAAA 
CAGCACCAAC AGCAGCAGCA GCCACAGCAG CCACCGCCAC CACAACCAGA GGCTTCTGGT 
TCATGGGGAG GCCCCCCACC ACCACCTCCA GGCAACGGAC GACCTTCCAA TTCCAACTGG 
AGCAGTGGGC CACAGCCAGT AACCCCTAAG GACGAGGAAC CCAGTGGTTG GGAAGAGCCA 
TCCCCACAGT CAATTAGTCG GAAAATGGAC ATCGATGATG GCACTTCAGC ATGGGGAGAC 
CCTAACAGCT ATAACTACAA GAATGTGAAT CTGTGGGATA AGAATTCCCA AGGGGGCCCA 
GCACCTCGAG AACCAAACCT GCCTACCCCG ATGACCAGTA AATCAGCATC AGATTCCAAA 
TCTATGCAAG ACGGCTGGGG GGAGAGTGAC GGTCCAGTGA CGGGAACTCG CCATCCCAGC 
TGGGAGGAGG AGGATGATGG AGGAGTCTGG AACACCGCCG GCTCTCAGGG AAGTGCTTCC 
TCCCACAACT CAGCAAGCTG GGGACAAGGA GGAAAGAAGC AAATGAAGTG CTCACTGAAA 
GGAGGAAACA ATGATTCATG GATGAATCCT CTTGCCAAAC AGTTTTCAAA TATGGGATTG 
CTGAGTCAAA CTGAAGATAA TCCAGGCAGC AAAATGGATT TGTCTGTAGG GAGCCTTCCA 
GATAAAAAAT TTGACGTGGA CAAGCGAGCA ATGAATCTCG GGGATTTTAA TGATATCATG 
AGGAAGGATC GATCTGGGTT CCGTCCACCT AATTCCAAAG ACATGGGAAC CACAGATAGT 
GGGCCTTATT TCGAGAAGGG TGGCAATCAT GGTTTGTTTG GAAATAGCAC AGCACAATCG 
AGAGGTATGC ACACACCAGT GCAGCCACTA AATTCTTCTC CTAATCTCCG GGCGCAAGTG 
CCTCCCCAGT TTATTTCCCC CCAGGTTTCT GCCTCCATGC TCAAGCAGTT TCCCAACAGT 
GGCCTGAATC CAGGTCTTTT CAATGTGGGG CCCCAGTTAT CTCCTCAACA GATTGCCATG 
CTGAGCCAGC TTCCACAAAT TCCCCAGTTT CAGTTGGCAT GTCAGCTTCT CCTGCAGCAG 
CAGCAACAGC AGCAGTTGCT ACAGAACCAG AGGAAGCTTT CTCAAGCTGT GCGCCAGCAG 
CAAGAGCAGC AGCTGGCTCG AATGGTGAGC GCTCTCCAGC AACAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGAGGCAGCC TGGCATGAAG CACTCACCCT CTCATCCTGT TGGGCCCAAG 
CCACATCTGG ACAACATGGT ACCCAACGCT CTGAATGTGG GGCTCCCAGA CCTTCAAACC 
AAAGGGCCAA TACCTGGATA TGGTTCTGGC TTCAGCTCTG GCGGCATGGA CTATGGCATG 
GTTGGTGGGA AGGAGGCTGG AACAGAGTCT CGCTTTAAAC AGTGGACCTC CATGATGGAG 
GGACTGCCCT CTGTAGCCAC ACAGGAAGCC AATATGCACA AAAATGGCGC TATTGTGGCC 
CCTGGTAAGA CTCGAGGAGG GTCACCGTAC AACCAGTTTG ATATCATCCC GAGTGACACA 
CTGGGTGGCC ATACGGGTCC TGCTGGTGAT AGCTGGTTAC CTGCCAAATC TCCACCAACA 
AATAAAATCG GAAGTAAATC CAGCAATGCC AGTTGGCCTC CAGAATTCCA ACCAGGAGTG 
CCATGGAAAG GTATCCAAAA CATTGACCCT GAATCTGACC CCTATGTCAC CCCAGGAAGT 
GTACTGGGGG GTACAGCCAC ATCTCCCATT GTAGATACTG ACCACCAACT TCTGCGGGAT 
AACACCACAG GGTCTAATTC TTCCCTCAAC ACCTCGCTGC CTTCACCTGG TGCCTGGCCC 
TACAGTGCCT CTGACAACTC CTTTACCAAC GTTCATAGCA CTTCAGCAAA GTTCCCTGAT 
TACAAATCAA CATGGTCCCC AGATCCCATA GGACACAACC CCACTCATCT CTCCAACAAG 
ATGTGGAAAA ACCATATTTC CTCAAGGAAC ACTACACCGC TGCCCCGCCC ACCTCCCGGT 
CTGACCAACC CCAAACCATC ATCTCCCTGG AGCAGCACAG CACCCCGATC AGTCAGGGGG 
TGGGGGACAC AGGACTCACG GCTTGCCTCG GCCTCTACCT GGAGTGATGG TGGTTCAGTT 
CGTCCCAGTT ACTGGCTGGT TCTTCACAAT CTCACTCCAC AGATTGATGG GTCAACGTTG 
AGAACCATCT GCATGCAGCA TGGCCCACTG CTGACATTCC ATCTGAACCT AACCCAGGGC 
ACTGCCCTGA TCCGATACAG CACCAAACAG GAGGCGGCCA AGGCCCAAAC TGCACTGCAC 
ATGTGTGTGT TGGGAAACAC TACCATCCTG GCTGAGTTTG CCACCGATGA TGAAGTTAGC 
CGCTTTCTGG CACAAGCTCA GCCCCCTACA CCTGCAGCAA CCCCAAGTGC ACCCGCCGCA 
GGTTGGCAGT CGCTGGAGAC CGGCCAGACC CAGTCAGATC CCGTCGGACC TGCTTTGAAT 
CTTTTTGGTG GGTCCACAGG GCTCGGGCAA TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG 
GCCGATCTTG CTGGCGCTTC GTTGTGGGGG CCCCCAAACT ATTCTTCTAG CTTGTGGGGA 
GTCCCAGCTG TGGAAGATCC CCATAGGATG GGCAGCCCTG CTCCCTTACT ACCTGGTGAC 
CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280103.1
CDS	16..5445
Translation	MDSHQDCFWLYDPPSSFHQSMSHHFPFHNRSQLHENTLCFYFNLFQGAVIQNPEGLLVEREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKAPDILKHAVNHTLILGCLKFGLQEAVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASDSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424395.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424395.1

ORF Insert Sequence:

ATGGACTCCC ATCAGGATTG CTTCTGGCTG TATGACCCAC CTTCCTCTTT CCATCAATCC 
ATGTCCCACC ATTTTCCATT CCATAACAGA AGTCAGCTTC ATGAGAATAC TCTTTGCTTC 
TACTTTAATC TCTTCCAGGG TGCAGTGATC CAGAATCCAG AAGGGCTGTT AGTTGAGAGA 
GAGAAGGAGC AAGAAAGGGA AGAACAGTTA ATGGAAGACA AGAAAAGGAA GAAAGAGGAT 
AAAAAGAAAA AAGAAGCCAC TCAGAAGGTC ACGGAACAAA AAACCAAAGC ACCTGACATT 
CTGAAGCATG CTGTGAACCA CACACTGATT CTGGGATGTC TTAAGTTTGG ACTTCAAGAA 
GCAGTGCCCG AAGTGACGAA ACCAAGTTTA AGCCAACCAA CGGCCGCCAG CCCAATTGGC 
AGCTCTCCAT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CAAGCGCCGC CCGCTACATG CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC 
CAGCAGGACC ACAAAGTGTT ACTAAAACGT GGGCAGCCCC CTCCGCCGTC CTGCATGCTC 
CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC ACAGCACCTG GAGCAAACTC AAACAACGCA 
CAAGTGACAG GAACGCTGCT GCAGAGTGAC AGTGGGACTG CACCAGAGTC AACCCTTGGA 
GGTGCTGCTG CTTCAAATTA TGCAAATTCC ACTTGGGGCC CGGGAGCCTC CTCCAACAAC 
GGCGCCTCCC CCAACCCAAT TCACACCTGG GACAAGGTGA TTGTAGACGG GTCTGACGTG 
GAAGAGTGGC CTTGTATTGC CAGCAAAGAC ACTGAGTCTT CTTCCGAAAA CACCACCGAT 
AACAACAGTG CCTCGAACCC TGGCTCTGAG AAGAGCACTC TGCCAGGAAG CACCACTAGT 
AACAAAGGAA AAGGGAGCCA GTGCCAGTCT GCAAGTTCTG GGAACGAATG TAATCTTGGG 
GTCTGGAAAT CTGACCCTAA GACTAAATCT GTTCAATCTT CCAACTCTAC AACAGAGAGC 
AACAATGGAC TAGGAAATTG GAGGAATGTG AGTGGTCAGG ATAGAATTGG ACCTGGCTCT 
GGCTTCAGCA ACTTTAACCC AAATAGCAAC CCATCTGCCT GGCCAGCGCT GGTCCAAGAA 
GGAAATTCTA GGAAAGGGGC ATTGGAAACG GATACTAGTA ATTCCAGTGC ACAGGTTAGC 
ACAGTAGGTC AGGCATCCAG GGAACAGCAG TCAAAGATGG AAAATGCGGG TGTTAATTTT 
GTTGTCTCTG GCAGAGAACA GGCTCAGATT CATAACACTG ATGGACCAAA AAATGGAAAC 
ACTAACTCCT TGAACTTAAG TTCACCAAAC CCCATGGAGA ATAAGGGAAT GCCCTTTGGA 
ATGGGCTTGG GGAACGCCTC CAGGAGCACT GATGCCCCTT CACAAAGCAC TGGAGATCGA 
AAGACTGGGA GTGTTGGATC TTGGGGTGCA GCTAGGGGGC CTTCTGGAAC TGACACAGCC 
TCTGGACAAA GCAATTCTGG AAACAATGGG AACAATGGAA AAGATAGAGA GGACTCCTGG 
AAAGGAGCTT CTGTTCAGAA ATCATCTGGG TCAAAAAATG ACTCTTGGGA CAATAACAGG 
TCTACGGGTG GGTCCTGGAA CTTTGGCCCT CAGGACTCTA ATGACAACAA ATGGGGTGAA 
GGGAACAAAA TGACATCTGG GGTCTCTCAG GGAGAATGGA AACAGCCGAC TGGGTCTGAT 
GAGTTGAAAA TTGGAGAATG GAGTGGTCCA AACCAACCAA ATTCTAGCAC TGGAGCATGG 
GACAATCAAA AGGGCCACCC CCTCCCTGAA AACCAAGGCA ATGCCCAGGC TCCCTGTTGG 
GGAAGATCTT CCAGCTCCAC AGGAAGTGAA GTTGGAGGTC AAAGCACTGG AAGCAACCAC 
AAAGCAGGAA GTAGTGACAG TCATAATTCT GGCCGTCGGT CATACCGGCC CACACATCCT 
GATTGTCAGG CTGTCTTACA GACTCTTTTG AGCCGAACTG ATTTGGACCC CAGGGTGCTC 
TCAAACACTG GCTGGGGCCA AACTCAAATT AAGCAGGACA CTGTGTGGGA TATTGAAGAG 
GTGCCAAGGC CTGAGGGGAA ATCGGACAAA GGAACTGAGG GGTGGGAGAG CGCTGCCACA 
CAGACCAAGA ACTCAGGGGG CTGGGGAGAT GCACCCAGCC AAAGCAATCA AATGAAGTCT 
GGATGGGGGG AGCTCTCAGC CTCTACAGAG TGGAAAGACC CCAAGAACAC AGGAGGCTGG 
AATGACTATA AGAACAACAA CTCTTCCAAC TGGGGAGGCG GACGACCAGA TGAAAAGACA 
TCTTCCTCTT GGAATGAGAA CTCCAGCAAG GATCAGGGGT GGGGAGGGGG ACGCCAGCCC 
AATCAAGGAT GGACTTCTGG GAAGAATGGT TGGGGAGAGG AAGTTGATCA GGCGAAAAAC 
AGCAGTTGGG AAAGTTCTGC AAGCAAACCT GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT 
GAAATTGGAA CTTGGGGGAA CAGCGGGAAT GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA 
GATTGCAAAA GGTCTCCAGC GTGGACTGAG ACTGGCAGAC AGCCCAATTC CTGGCATAAA 
CAGCACCAAC AGCAGCAGCA GCCACAGCAG CCACCGCCAC CACAACCAGA GGCTTCTGGT 
TCATGGGGAG GCCCCCCACC ACCACCTCCA GGCAACGGAC GACCTTCCAA TTCCAACTGG 
AGCAGTGGGC CACAGCCAGT AACCCCTAAG GACGAGGAAC CCAGTGGTTG GGAAGAGCCA 
TCCCCACAGT CAATTAGTCG GAAAATGGAC ATCGATGATG GCACTTCAGC ATGGGGAGAC 
CCTAACAGCT ATAACTACAA GAATGTGAAT CTGTGGGATA AGAATTCCCA AGGGGGCCCA 
GCACCTCGAG AACCAAACCT GCCTACCCCG ATGACCAGTA AATCAGCATC AGATTCCAAA 
TCTATGCAAG ACGGCTGGGG GGAGAGTGAC GGTCCAGTGA CGGGAACTCG CCATCCCAGC 
TGGGAGGAGG AGGATGATGG AGGAGTCTGG AACACCGCCG GCTCTCAGGG AAGTGCTTCC 
TCCCACAACT CAGCAAGCTG GGGACAAGGA GGAAAGAAGC AAATGAAGTG CTCACTGAAA 
GGAGGAAACA ATGATTCATG GATGAATCCT CTTGCCAAAC AGTTTTCAAA TATGGGATTG 
CTGAGTCAAA CTGAAGATAA TCCAGGCAGC AAAATGGATT TGTCTGTAGG GAGCCTTCCA 
GATAAAAAAT TTGACGTGGA CAAGCGAGCA ATGAATCTCG GGGATTTTAA TGATATCATG 
AGGAAGGATC GATCTGGGTT CCGTCCACCT AATTCCAAAG ACATGGGAAC CACAGATAGT 
GGGCCTTATT TCGAGAAGGG TGGCAATCAT GGTTTGTTTG GAAATAGCAC AGCACAATCG 
AGAGGTATGC ACACACCAGT GCAGCCACTA AATTCTTCTC CTAATCTCCG GGCGCAAGTG 
CCTCCCCAGT TTATTTCCCC CCAGGTTTCT GCCTCCATGC TCAAGCAGTT TCCCAACAGT 
GGCCTGAATC CAGGTCTTTT CAATGTGGGG CCCCAGTTAT CTCCTCAACA GATTGCCATG 
CTGAGCCAGC TTCCACAAAT TCCCCAGTTT CAGTTGGCAT GTCAGCTTCT CCTGCAGCAG 
CAGCAACAGC AGCAGTTGCT ACAGAACCAG AGGAAGCTTT CTCAAGCTGT GCGCCAGCAG 
CAAGAGCAGC AGCTGGCTCG AATGGTGAGC GCTCTCCAGC AACAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGAGGCAGCC TGGCATGAAG CACTCACCCT CTCATCCTGT TGGGCCCAAG 
CCACATCTGG ACAACATGGT ACCCAACGCT CTGAATGTGG GGCTCCCAGA CCTTCAAACC 
AAAGGGCCAA TACCTGGATA TGGTTCTGGC TTCAGCTCTG GCGGCATGGA CTATGGCATG 
GTTGGTGGGA AGGAGGCTGG AACAGAGTCT CGCTTTAAAC AGTGGACCTC CATGATGGAG 
GGACTGCCCT CTGTAGCCAC ACAGGAAGCC AATATGCACA AAAATGGCGC TATTGTGGCC 
CCTGGTAAGA CTCGAGGAGG GTCACCGTAC AACCAGTTTG ATATCATCCC GAGTGACACA 
CTGGGTGGCC ATACGGGTCC TGCTGGTGAT AGCTGGTTAC CTGCCAAATC TCCACCAACA 
AATAAAATCG GAAGTAAATC CAGCAATGCC AGTTGGCCTC CAGAATTCCA ACCAGGAGTG 
CCATGGAAAG GTATCCAAAA CATTGACCCT GAATCTGACC CCTATGTCAC CCCAGGAAGT 
GTACTGGGGG GTACAGCCAC ATCTCCCATT GTAGATACTG ACCACCAACT TCTGCGGGAT 
AACACCACAG GGTCTAATTC TTCCCTCAAC ACCTCGCTGC CTTCACCTGG TGCCTGGCCC 
TACAGTGCCT CTGACAACTC CTTTACCAAC GTTCATAGCA CTTCAGCAAA GTTCCCTGAT 
TACAAATCAA CATGGTCCCC AGATCCCATA GGACACAACC CCACTCATCT CTCCAACAAG 
ATGTGGAAAA ACCATATTTC CTCAAGGAAC ACTACACCGC TGCCCCGCCC ACCTCCCGGT 
CTGACCAACC CCAAACCATC ATCTCCCTGG AGCAGCACAG CACCCCGATC AGTCAGGGGG 
TGGGGGACAC AGGACTCACG GCTTGCCTCG GCCTCTACCT GGAGTGATGG TGGTTCAGTT 
CGTCCCAGTT ACTGGCTGGT TCTTCACAAT CTCACTCCAC AGATTGATGG GTCAACGTTG 
AGAACCATCT GCATGCAGCA TGGCCCACTG CTGACATTCC ATCTGAACCT AACCCAGGGC 
ACTGCCCTGA TCCGATACAG CACCAAACAG GAGGCGGCCA AGGCCCAAAC TGCACTGCAC 
ATGTGTGTGT TGGGAAACAC TACCATCCTG GCTGAGTTTG CCACCGATGA TGAAGTTAGC 
CGCTTTCTGG CACAAGCTCA GCCCCCTACA CCTGCAGCAA CCCCAAGTGC ACCCGCCGCA 
GGTTGGCAGT CGCTGGAGAC CGGCCAGACC CAGTCAGATC CCGTCGGACC TGCTTTGAAT 
CTTTTTGGTG GGTCCACAGG GCTCGGGCAA TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG 
GCCGATCTTG CTGGCGCTTC GTTGTGGGGG CCCCCAAACT ATTCTTCTAG CTTGTGGGGA 
GTCCCAGCTG TGGAAGATCC CCATAGGATG GGCAGCCCTG CTCCCTTACT ACCTGGTGAC 
CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_014117339.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972814.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

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CloneID	OCe38893
Clone ID Related Accession (Same CDS sequence)	XM_014117339.1 , XM_014117340.1 , XM_014117339.2
Accession Version	XM_014117339.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GTCTGGAGCA AAAGCACACC ACCTGCTCCA 
GATAATGGTA CTTCAGCTTG GGGTGAGCCA AACGAAAGCA GTCCTGGGTG GGGCGAAATG 
GATGACACGG GAGCATCGAC CACGGGCTGG GGGAATGCAC CCTCCAACGC TCCAAATGCC 
ATGAAACCTA ATTCCAAATC TATGCAAGAC GGCTGGGGGG AGAGTGACGG TCCAGTGACG 
GGAACTCGCC ATCCCAGCTG GGAGGAGGAG GATGATGGAG GAGTCTGGAA CACCGCCGGC 
TCTCAGGGAA GTGCTTCCTC CCACAACTCA GCAAGCTGGG GACAAGGAGG AAAGAAGCAA 
ATGAAGTGCT CACTGAAAGG AGGAAACAAT GATTCATGGA TGAATCCTCT TGCCAAACAG 
TTTTCAAATA TGGGATTGCT GAGTCAAACT GAAGATAATC CAGGCAGCAA AATGGATTTG 
TCTGTAGGGA GCCTTCCAGA TAAAAAATTT GACGTGGACA AGCGAGCAAT GAATCTCGGG 
GATTTTAATG ATATCATGAG GAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTC GAGAAGCTGA CTTTGCCTTT CTCCAATCAA 
GATGGGTGCC TTGGGGATGA GGCTCCCTGC TCTCCCTTCT CCCCTTCTCC CAGCTACAAG 
CTGTCTCCCT CTGGTTCCAC ACTACCCAAC GTCAGCCTTG GAGCAATCGG CACAGGGCTC 
AACCCCCAAA ACTTCGCTGC TAGACAAGGT GGCAATCATG GTTTGTTTGG AAATAGCACA 
GCACAATCGA GAGGTATGCA CACACCAGTG CAGCCACTAA ATTCTTCTCC TAATCTCCGG 
GCGCAAGTGC CTCCCCAGTT TATTTCCCCC CAGGTTTCTG CCTCCATGCT CAAGCAGTTT 
CCCAACAGTG GCCTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGTTATC TCCTCAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAAATT CCCCAGTTTC AGTTGGCATG TCAGCTTCTC 
CTGCAGCAGC AGCAACAGCA GCAGTTGCTA CAGAACCAGA GGAAGCTTTC TCAAGCTGTG 
CGCCAGCAGC AAGAGCAGCA GCTGGCTCGA ATGGTGAGCG CTCTCCAGCA ACAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GAGGCAGCCT GGCATGAAGC ACTCACCCTC TCATCCTGTT 
GGGCCCAAGC CACATCTGGA CAACATGGTA CCCAACGCTC TGAATGTGGG GCTCCCAGAC 
CTTCAAACCA AAGGGCCAAT ACCTGGATAT GGTTCTGGCT TCAGCTCTGG CGGCATGGAC 
TATGGCATGG TTGGTGGGAA GGAGGCTGGA ACAGAGTCTC GCTTTAAACA GTGGACCTCC 
ATGATGGAGG GACTGCCCTC TGTAGCCACA CAGGAAGCCA ATATGCACAA AAATGGCGCT 
ATTGTGGCCC CTGGTAAGAC TCGAGGAGGG TCACCGTACA ACCAGTTTGA TATCATCCCG 
AGTGACACAC TGGGTGGCCA TACGGGTCCT GCTGGTGATA GCTGGTTACC TGCCAAATCT 
CCACCAACAA ATAAAATCGG AAGTAAATCC AGCAATGCCA GTTGGCCTCC AGAATTCCAA 
CCAGGAGTGC CATGGAAAGG TATCCAAAAC ATTGACCCTG AATCTGACCC CTATGTCACC 
CCAGGAAGTG TACTGGGGGG TACAGCCACA TCTCCCATTG TAGATACTGA CCACCAACTT 
CTGCGGGATA ACACCACAGG GTCTAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT ACAGTGCCTC TGACAACTCC TTTACCAACG TTCATAGCAC TTCAGCAAAG 
TTCCCTGATT ACAAATCAAC ATGGTCCCCA GATCCCATAG GACACAACCC CACTCATCTC 
TCCAACAAGA TGTGGAAAAA CCATATTTCC TCAAGGAACA CTACACCGCT GCCCCGCCCA 
CCTCCCGGTC TGACCAACCC CAAACCATCA TCTCCCTGGA GCAGCACAGC ACCCCGATCA 
GTCAGGGGGT GGGGGACACA GGACTCACGG CTTGCCTCGG CCTCTACCTG GAGTGATGGT 
GGTTCAGTTC GTCCCAGTTA CTGGCTGGTT CTTCACAATC TCACTCCACA GATTGATGGG 
TCAACGTTGA GAACCATCTG CATGCAGCAT GGCCCACTGC TGACATTCCA TCTGAACCTA 
ACCCAGGGCA CTGCCCTGAT CCGATACAGC ACCAAACAGG AGGCGGCCAA GGCCCAAACT 
GCACTGCACA TGTGTGTGTT GGGAAACACT ACCATCCTGG CTGAGTTTGC CACCGATGAT 
GAAGTTAGCC GCTTTCTGGC ACAAGCTCAG CCCCCTACAC CTGCAGCAAC CCCAAGTGCA 
CCCGCCGCAG GTTGGCAGTC GCTGGAGACC GGCCAGACCC AGTCAGATCC CGTCGGACCT 
GCTTTGAATC TTTTTGGTGG GTCCACAGGG CTCGGGCAAT GGAGCAGCAG TGCTGGTGGC 
AGCAGCGGGG CCGATCTTGC TGGCGCTTCG TTGTGGGGGC CCCCAAACTA TTCTTCTAGC 
TTGTGGGGAG TCCCAGCTGT GGAAGATCCC CATAGGATGG GCAGCCCTGC TCCCTTACTA 
CCTGGTGACC TTCTGGGAGG AGGGTCGGAT TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972814.1
CDS	245..5863
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_014117339.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117339.1

ORF Insert Sequence:

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GTCTGGAGCA AAAGCACACC ACCTGCTCCA 
GATAATGGTA CTTCAGCTTG GGGTGAGCCA AACGAAAGCA GTCCTGGGTG GGGCGAAATG 
GATGACACGG GAGCATCGAC CACGGGCTGG GGGAATGCAC CCTCCAACGC TCCAAATGCC 
ATGAAACCTA ATTCCAAATC TATGCAAGAC GGCTGGGGGG AGAGTGACGG TCCAGTGACG 
GGAACTCGCC ATCCCAGCTG GGAGGAGGAG GATGATGGAG GAGTCTGGAA CACCGCCGGC 
TCTCAGGGAA GTGCTTCCTC CCACAACTCA GCAAGCTGGG GACAAGGAGG AAAGAAGCAA 
ATGAAGTGCT CACTGAAAGG AGGAAACAAT GATTCATGGA TGAATCCTCT TGCCAAACAG 
TTTTCAAATA TGGGATTGCT GAGTCAAACT GAAGATAATC CAGGCAGCAA AATGGATTTG 
TCTGTAGGGA GCCTTCCAGA TAAAAAATTT GACGTGGACA AGCGAGCAAT GAATCTCGGG 
GATTTTAATG ATATCATGAG GAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTC GAGAAGCTGA CTTTGCCTTT CTCCAATCAA 
GATGGGTGCC TTGGGGATGA GGCTCCCTGC TCTCCCTTCT CCCCTTCTCC CAGCTACAAG 
CTGTCTCCCT CTGGTTCCAC ACTACCCAAC GTCAGCCTTG GAGCAATCGG CACAGGGCTC 
AACCCCCAAA ACTTCGCTGC TAGACAAGGT GGCAATCATG GTTTGTTTGG AAATAGCACA 
GCACAATCGA GAGGTATGCA CACACCAGTG CAGCCACTAA ATTCTTCTCC TAATCTCCGG 
GCGCAAGTGC CTCCCCAGTT TATTTCCCCC CAGGTTTCTG CCTCCATGCT CAAGCAGTTT 
CCCAACAGTG GCCTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGTTATC TCCTCAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAAATT CCCCAGTTTC AGTTGGCATG TCAGCTTCTC 
CTGCAGCAGC AGCAACAGCA GCAGTTGCTA CAGAACCAGA GGAAGCTTTC TCAAGCTGTG 
CGCCAGCAGC AAGAGCAGCA GCTGGCTCGA ATGGTGAGCG CTCTCCAGCA ACAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GAGGCAGCCT GGCATGAAGC ACTCACCCTC TCATCCTGTT 
GGGCCCAAGC CACATCTGGA CAACATGGTA CCCAACGCTC TGAATGTGGG GCTCCCAGAC 
CTTCAAACCA AAGGGCCAAT ACCTGGATAT GGTTCTGGCT TCAGCTCTGG CGGCATGGAC 
TATGGCATGG TTGGTGGGAA GGAGGCTGGA ACAGAGTCTC GCTTTAAACA GTGGACCTCC 
ATGATGGAGG GACTGCCCTC TGTAGCCACA CAGGAAGCCA ATATGCACAA AAATGGCGCT 
ATTGTGGCCC CTGGTAAGAC TCGAGGAGGG TCACCGTACA ACCAGTTTGA TATCATCCCG 
AGTGACACAC TGGGTGGCCA TACGGGTCCT GCTGGTGATA GCTGGTTACC TGCCAAATCT 
CCACCAACAA ATAAAATCGG AAGTAAATCC AGCAATGCCA GTTGGCCTCC AGAATTCCAA 
CCAGGAGTGC CATGGAAAGG TATCCAAAAC ATTGACCCTG AATCTGACCC CTATGTCACC 
CCAGGAAGTG TACTGGGGGG TACAGCCACA TCTCCCATTG TAGATACTGA CCACCAACTT 
CTGCGGGATA ACACCACAGG GTCTAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT ACAGTGCCTC TGACAACTCC TTTACCAACG TTCATAGCAC TTCAGCAAAG 
TTCCCTGATT ACAAATCAAC ATGGTCCCCA GATCCCATAG GACACAACCC CACTCATCTC 
TCCAACAAGA TGTGGAAAAA CCATATTTCC TCAAGGAACA CTACACCGCT GCCCCGCCCA 
CCTCCCGGTC TGACCAACCC CAAACCATCA TCTCCCTGGA GCAGCACAGC ACCCCGATCA 
GTCAGGGGGT GGGGGACACA GGACTCACGG CTTGCCTCGG CCTCTACCTG GAGTGATGGT 
GGTTCAGTTC GTCCCAGTTA CTGGCTGGTT CTTCACAATC TCACTCCACA GATTGATGGG 
TCAACGTTGA GAACCATCTG CATGCAGCAT GGCCCACTGC TGACATTCCA TCTGAACCTA 
ACCCAGGGCA CTGCCCTGAT CCGATACAGC ACCAAACAGG AGGCGGCCAA GGCCCAAACT 
GCACTGCACA TGTGTGTGTT GGGAAACACT ACCATCCTGG CTGAGTTTGC CACCGATGAT 
GAAGTTAGCC GCTTTCTGGC ACAAGCTCAG CCCCCTACAC CTGCAGCAAC CCCAAGTGCA 
CCCGCCGCAG GTTGGCAGTC GCTGGAGACC GGCCAGACCC AGTCAGATCC CGTCGGACCT 
GCTTTGAATC TTTTTGGTGG GTCCACAGGG CTCGGGCAAT GGAGCAGCAG TGCTGGTGGC 
AGCAGCGGGG CCGATCTTGC TGGCGCTTCG TTGTGGGGGC CCCCAAACTA TTCTTCTAGC 
TTGTGGGGAG TCCCAGCTGT GGAAGATCCC CATAGGATGG GCAGCCCTGC TCCCTTACTA 
CCTGGTGACC TTCTGGGAGG AGGGTCGGAT TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_014117340.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972815.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

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CloneID	OCe38893
Clone ID Related Accession (Same CDS sequence)	XM_014117339.1 , XM_014117340.1 , XM_014117339.2
Accession Version	XM_014117340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GTCTGGAGCA AAAGCACACC ACCTGCTCCA 
GATAATGGTA CTTCAGCTTG GGGTGAGCCA AACGAAAGCA GTCCTGGGTG GGGCGAAATG 
GATGACACGG GAGCATCGAC CACGGGCTGG GGGAATGCAC CCTCCAACGC TCCAAATGCC 
ATGAAACCTA ATTCCAAATC TATGCAAGAC GGCTGGGGGG AGAGTGACGG TCCAGTGACG 
GGAACTCGCC ATCCCAGCTG GGAGGAGGAG GATGATGGAG GAGTCTGGAA CACCGCCGGC 
TCTCAGGGAA GTGCTTCCTC CCACAACTCA GCAAGCTGGG GACAAGGAGG AAAGAAGCAA 
ATGAAGTGCT CACTGAAAGG AGGAAACAAT GATTCATGGA TGAATCCTCT TGCCAAACAG 
TTTTCAAATA TGGGATTGCT GAGTCAAACT GAAGATAATC CAGGCAGCAA AATGGATTTG 
TCTGTAGGGA GCCTTCCAGA TAAAAAATTT GACGTGGACA AGCGAGCAAT GAATCTCGGG 
GATTTTAATG ATATCATGAG GAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTC GAGAAGCTGA CTTTGCCTTT CTCCAATCAA 
GATGGGTGCC TTGGGGATGA GGCTCCCTGC TCTCCCTTCT CCCCTTCTCC CAGCTACAAG 
CTGTCTCCCT CTGGTTCCAC ACTACCCAAC GTCAGCCTTG GAGCAATCGG CACAGGGCTC 
AACCCCCAAA ACTTCGCTGC TAGACAAGGT GGCAATCATG GTTTGTTTGG AAATAGCACA 
GCACAATCGA GAGGTATGCA CACACCAGTG CAGCCACTAA ATTCTTCTCC TAATCTCCGG 
GCGCAAGTGC CTCCCCAGTT TATTTCCCCC CAGGTTTCTG CCTCCATGCT CAAGCAGTTT 
CCCAACAGTG GCCTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGTTATC TCCTCAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAAATT CCCCAGTTTC AGTTGGCATG TCAGCTTCTC 
CTGCAGCAGC AGCAACAGCA GCAGTTGCTA CAGAACCAGA GGAAGCTTTC TCAAGCTGTG 
CGCCAGCAGC AAGAGCAGCA GCTGGCTCGA ATGGTGAGCG CTCTCCAGCA ACAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GAGGCAGCCT GGCATGAAGC ACTCACCCTC TCATCCTGTT 
GGGCCCAAGC CACATCTGGA CAACATGGTA CCCAACGCTC TGAATGTGGG GCTCCCAGAC 
CTTCAAACCA AAGGGCCAAT ACCTGGATAT GGTTCTGGCT TCAGCTCTGG CGGCATGGAC 
TATGGCATGG TTGGTGGGAA GGAGGCTGGA ACAGAGTCTC GCTTTAAACA GTGGACCTCC 
ATGATGGAGG GACTGCCCTC TGTAGCCACA CAGGAAGCCA ATATGCACAA AAATGGCGCT 
ATTGTGGCCC CTGGTAAGAC TCGAGGAGGG TCACCGTACA ACCAGTTTGA TATCATCCCG 
AGTGACACAC TGGGTGGCCA TACGGGTCCT GCTGGTGATA GCTGGTTACC TGCCAAATCT 
CCACCAACAA ATAAAATCGG AAGTAAATCC AGCAATGCCA GTTGGCCTCC AGAATTCCAA 
CCAGGAGTGC CATGGAAAGG TATCCAAAAC ATTGACCCTG AATCTGACCC CTATGTCACC 
CCAGGAAGTG TACTGGGGGG TACAGCCACA TCTCCCATTG TAGATACTGA CCACCAACTT 
CTGCGGGATA ACACCACAGG GTCTAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT ACAGTGCCTC TGACAACTCC TTTACCAACG TTCATAGCAC TTCAGCAAAG 
TTCCCTGATT ACAAATCAAC ATGGTCCCCA GATCCCATAG GACACAACCC CACTCATCTC 
TCCAACAAGA TGTGGAAAAA CCATATTTCC TCAAGGAACA CTACACCGCT GCCCCGCCCA 
CCTCCCGGTC TGACCAACCC CAAACCATCA TCTCCCTGGA GCAGCACAGC ACCCCGATCA 
GTCAGGGGGT GGGGGACACA GGACTCACGG CTTGCCTCGG CCTCTACCTG GAGTGATGGT 
GGTTCAGTTC GTCCCAGTTA CTGGCTGGTT CTTCACAATC TCACTCCACA GATTGATGGG 
TCAACGTTGA GAACCATCTG CATGCAGCAT GGCCCACTGC TGACATTCCA TCTGAACCTA 
ACCCAGGGCA CTGCCCTGAT CCGATACAGC ACCAAACAGG AGGCGGCCAA GGCCCAAACT 
GCACTGCACA TGTGTGTGTT GGGAAACACT ACCATCCTGG CTGAGTTTGC CACCGATGAT 
GAAGTTAGCC GCTTTCTGGC ACAAGCTCAG CCCCCTACAC CTGCAGCAAC CCCAAGTGCA 
CCCGCCGCAG GTTGGCAGTC GCTGGAGACC GGCCAGACCC AGTCAGATCC CGTCGGACCT 
GCTTTGAATC TTTTTGGTGG GTCCACAGGG CTCGGGCAAT GGAGCAGCAG TGCTGGTGGC 
AGCAGCGGGG CCGATCTTGC TGGCGCTTCG TTGTGGGGGC CCCCAAACTA TTCTTCTAGC 
TTGTGGGGAG TCCCAGCTGT GGAAGATCCC CATAGGATGG GCAGCCCTGC TCCCTTACTA 
CCTGGTGACC TTCTGGGAGG AGGGTCGGAT TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972815.1
CDS	2538..8156
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_014117340.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117340.1

ORF Insert Sequence:

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GTCTGGAGCA AAAGCACACC ACCTGCTCCA 
GATAATGGTA CTTCAGCTTG GGGTGAGCCA AACGAAAGCA GTCCTGGGTG GGGCGAAATG 
GATGACACGG GAGCATCGAC CACGGGCTGG GGGAATGCAC CCTCCAACGC TCCAAATGCC 
ATGAAACCTA ATTCCAAATC TATGCAAGAC GGCTGGGGGG AGAGTGACGG TCCAGTGACG 
GGAACTCGCC ATCCCAGCTG GGAGGAGGAG GATGATGGAG GAGTCTGGAA CACCGCCGGC 
TCTCAGGGAA GTGCTTCCTC CCACAACTCA GCAAGCTGGG GACAAGGAGG AAAGAAGCAA 
ATGAAGTGCT CACTGAAAGG AGGAAACAAT GATTCATGGA TGAATCCTCT TGCCAAACAG 
TTTTCAAATA TGGGATTGCT GAGTCAAACT GAAGATAATC CAGGCAGCAA AATGGATTTG 
TCTGTAGGGA GCCTTCCAGA TAAAAAATTT GACGTGGACA AGCGAGCAAT GAATCTCGGG 
GATTTTAATG ATATCATGAG GAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTC GAGAAGCTGA CTTTGCCTTT CTCCAATCAA 
GATGGGTGCC TTGGGGATGA GGCTCCCTGC TCTCCCTTCT CCCCTTCTCC CAGCTACAAG 
CTGTCTCCCT CTGGTTCCAC ACTACCCAAC GTCAGCCTTG GAGCAATCGG CACAGGGCTC 
AACCCCCAAA ACTTCGCTGC TAGACAAGGT GGCAATCATG GTTTGTTTGG AAATAGCACA 
GCACAATCGA GAGGTATGCA CACACCAGTG CAGCCACTAA ATTCTTCTCC TAATCTCCGG 
GCGCAAGTGC CTCCCCAGTT TATTTCCCCC CAGGTTTCTG CCTCCATGCT CAAGCAGTTT 
CCCAACAGTG GCCTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGTTATC TCCTCAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAAATT CCCCAGTTTC AGTTGGCATG TCAGCTTCTC 
CTGCAGCAGC AGCAACAGCA GCAGTTGCTA CAGAACCAGA GGAAGCTTTC TCAAGCTGTG 
CGCCAGCAGC AAGAGCAGCA GCTGGCTCGA ATGGTGAGCG CTCTCCAGCA ACAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GAGGCAGCCT GGCATGAAGC ACTCACCCTC TCATCCTGTT 
GGGCCCAAGC CACATCTGGA CAACATGGTA CCCAACGCTC TGAATGTGGG GCTCCCAGAC 
CTTCAAACCA AAGGGCCAAT ACCTGGATAT GGTTCTGGCT TCAGCTCTGG CGGCATGGAC 
TATGGCATGG TTGGTGGGAA GGAGGCTGGA ACAGAGTCTC GCTTTAAACA GTGGACCTCC 
ATGATGGAGG GACTGCCCTC TGTAGCCACA CAGGAAGCCA ATATGCACAA AAATGGCGCT 
ATTGTGGCCC CTGGTAAGAC TCGAGGAGGG TCACCGTACA ACCAGTTTGA TATCATCCCG 
AGTGACACAC TGGGTGGCCA TACGGGTCCT GCTGGTGATA GCTGGTTACC TGCCAAATCT 
CCACCAACAA ATAAAATCGG AAGTAAATCC AGCAATGCCA GTTGGCCTCC AGAATTCCAA 
CCAGGAGTGC CATGGAAAGG TATCCAAAAC ATTGACCCTG AATCTGACCC CTATGTCACC 
CCAGGAAGTG TACTGGGGGG TACAGCCACA TCTCCCATTG TAGATACTGA CCACCAACTT 
CTGCGGGATA ACACCACAGG GTCTAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT ACAGTGCCTC TGACAACTCC TTTACCAACG TTCATAGCAC TTCAGCAAAG 
TTCCCTGATT ACAAATCAAC ATGGTCCCCA GATCCCATAG GACACAACCC CACTCATCTC 
TCCAACAAGA TGTGGAAAAA CCATATTTCC TCAAGGAACA CTACACCGCT GCCCCGCCCA 
CCTCCCGGTC TGACCAACCC CAAACCATCA TCTCCCTGGA GCAGCACAGC ACCCCGATCA 
GTCAGGGGGT GGGGGACACA GGACTCACGG CTTGCCTCGG CCTCTACCTG GAGTGATGGT 
GGTTCAGTTC GTCCCAGTTA CTGGCTGGTT CTTCACAATC TCACTCCACA GATTGATGGG 
TCAACGTTGA GAACCATCTG CATGCAGCAT GGCCCACTGC TGACATTCCA TCTGAACCTA 
ACCCAGGGCA CTGCCCTGAT CCGATACAGC ACCAAACAGG AGGCGGCCAA GGCCCAAACT 
GCACTGCACA TGTGTGTGTT GGGAAACACT ACCATCCTGG CTGAGTTTGC CACCGATGAT 
GAAGTTAGCC GCTTTCTGGC ACAAGCTCAG CCCCCTACAC CTGCAGCAAC CCCAAGTGCA 
CCCGCCGCAG GTTGGCAGTC GCTGGAGACC GGCCAGACCC AGTCAGATCC CGTCGGACCT 
GCTTTGAATC TTTTTGGTGG GTCCACAGGG CTCGGGCAAT GGAGCAGCAG TGCTGGTGGC 
AGCAGCGGGG CCGATCTTGC TGGCGCTTCG TTGTGGGGGC CCCCAAACTA TTCTTCTAGC 
TTGTGGGGAG TCCCAGCTGT GGAAGATCCC CATAGGATGG GCAGCCCTGC TCCCTTACTA 
CCTGGTGACC TTCTGGGAGG AGGGTCGGAT TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_014117339.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972814.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

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CloneID	OCe38893
Clone ID Related Accession (Same CDS sequence)	XM_014117339.1 , XM_014117340.1 , XM_014117339.2
Accession Version	XM_014117339.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117339.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GTCTGGAGCA AAAGCACACC ACCTGCTCCA 
GATAATGGTA CTTCAGCTTG GGGTGAGCCA AACGAAAGCA GTCCTGGGTG GGGCGAAATG 
GATGACACGG GAGCATCGAC CACGGGCTGG GGGAATGCAC CCTCCAACGC TCCAAATGCC 
ATGAAACCTA ATTCCAAATC TATGCAAGAC GGCTGGGGGG AGAGTGACGG TCCAGTGACG 
GGAACTCGCC ATCCCAGCTG GGAGGAGGAG GATGATGGAG GAGTCTGGAA CACCGCCGGC 
TCTCAGGGAA GTGCTTCCTC CCACAACTCA GCAAGCTGGG GACAAGGAGG AAAGAAGCAA 
ATGAAGTGCT CACTGAAAGG AGGAAACAAT GATTCATGGA TGAATCCTCT TGCCAAACAG 
TTTTCAAATA TGGGATTGCT GAGTCAAACT GAAGATAATC CAGGCAGCAA AATGGATTTG 
TCTGTAGGGA GCCTTCCAGA TAAAAAATTT GACGTGGACA AGCGAGCAAT GAATCTCGGG 
GATTTTAATG ATATCATGAG GAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTC GAGAAGCTGA CTTTGCCTTT CTCCAATCAA 
GATGGGTGCC TTGGGGATGA GGCTCCCTGC TCTCCCTTCT CCCCTTCTCC CAGCTACAAG 
CTGTCTCCCT CTGGTTCCAC ACTACCCAAC GTCAGCCTTG GAGCAATCGG CACAGGGCTC 
AACCCCCAAA ACTTCGCTGC TAGACAAGGT GGCAATCATG GTTTGTTTGG AAATAGCACA 
GCACAATCGA GAGGTATGCA CACACCAGTG CAGCCACTAA ATTCTTCTCC TAATCTCCGG 
GCGCAAGTGC CTCCCCAGTT TATTTCCCCC CAGGTTTCTG CCTCCATGCT CAAGCAGTTT 
CCCAACAGTG GCCTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGTTATC TCCTCAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAAATT CCCCAGTTTC AGTTGGCATG TCAGCTTCTC 
CTGCAGCAGC AGCAACAGCA GCAGTTGCTA CAGAACCAGA GGAAGCTTTC TCAAGCTGTG 
CGCCAGCAGC AAGAGCAGCA GCTGGCTCGA ATGGTGAGCG CTCTCCAGCA ACAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GAGGCAGCCT GGCATGAAGC ACTCACCCTC TCATCCTGTT 
GGGCCCAAGC CACATCTGGA CAACATGGTA CCCAACGCTC TGAATGTGGG GCTCCCAGAC 
CTTCAAACCA AAGGGCCAAT ACCTGGATAT GGTTCTGGCT TCAGCTCTGG CGGCATGGAC 
TATGGCATGG TTGGTGGGAA GGAGGCTGGA ACAGAGTCTC GCTTTAAACA GTGGACCTCC 
ATGATGGAGG GACTGCCCTC TGTAGCCACA CAGGAAGCCA ATATGCACAA AAATGGCGCT 
ATTGTGGCCC CTGGTAAGAC TCGAGGAGGG TCACCGTACA ACCAGTTTGA TATCATCCCG 
AGTGACACAC TGGGTGGCCA TACGGGTCCT GCTGGTGATA GCTGGTTACC TGCCAAATCT 
CCACCAACAA ATAAAATCGG AAGTAAATCC AGCAATGCCA GTTGGCCTCC AGAATTCCAA 
CCAGGAGTGC CATGGAAAGG TATCCAAAAC ATTGACCCTG AATCTGACCC CTATGTCACC 
CCAGGAAGTG TACTGGGGGG TACAGCCACA TCTCCCATTG TAGATACTGA CCACCAACTT 
CTGCGGGATA ACACCACAGG GTCTAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT ACAGTGCCTC TGACAACTCC TTTACCAACG TTCATAGCAC TTCAGCAAAG 
TTCCCTGATT ACAAATCAAC ATGGTCCCCA GATCCCATAG GACACAACCC CACTCATCTC 
TCCAACAAGA TGTGGAAAAA CCATATTTCC TCAAGGAACA CTACACCGCT GCCCCGCCCA 
CCTCCCGGTC TGACCAACCC CAAACCATCA TCTCCCTGGA GCAGCACAGC ACCCCGATCA 
GTCAGGGGGT GGGGGACACA GGACTCACGG CTTGCCTCGG CCTCTACCTG GAGTGATGGT 
GGTTCAGTTC GTCCCAGTTA CTGGCTGGTT CTTCACAATC TCACTCCACA GATTGATGGG 
TCAACGTTGA GAACCATCTG CATGCAGCAT GGCCCACTGC TGACATTCCA TCTGAACCTA 
ACCCAGGGCA CTGCCCTGAT CCGATACAGC ACCAAACAGG AGGCGGCCAA GGCCCAAACT 
GCACTGCACA TGTGTGTGTT GGGAAACACT ACCATCCTGG CTGAGTTTGC CACCGATGAT 
GAAGTTAGCC GCTTTCTGGC ACAAGCTCAG CCCCCTACAC CTGCAGCAAC CCCAAGTGCA 
CCCGCCGCAG GTTGGCAGTC GCTGGAGACC GGCCAGACCC AGTCAGATCC CGTCGGACCT 
GCTTTGAATC TTTTTGGTGG GTCCACAGGG CTCGGGCAAT GGAGCAGCAG TGCTGGTGGC 
AGCAGCGGGG CCGATCTTGC TGGCGCTTCG TTGTGGGGGC CCCCAAACTA TTCTTCTAGC 
TTGTGGGGAG TCCCAGCTGT GGAAGATCCC CATAGGATGG GCAGCCCTGC TCCCTTACTA 
CCTGGTGACC TTCTGGGAGG AGGGTCGGAT TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972814.1
CDS	204..5822
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_014117339.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117339.2

ORF Insert Sequence:

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GTCTGGAGCA AAAGCACACC ACCTGCTCCA 
GATAATGGTA CTTCAGCTTG GGGTGAGCCA AACGAAAGCA GTCCTGGGTG GGGCGAAATG 
GATGACACGG GAGCATCGAC CACGGGCTGG GGGAATGCAC CCTCCAACGC TCCAAATGCC 
ATGAAACCTA ATTCCAAATC TATGCAAGAC GGCTGGGGGG AGAGTGACGG TCCAGTGACG 
GGAACTCGCC ATCCCAGCTG GGAGGAGGAG GATGATGGAG GAGTCTGGAA CACCGCCGGC 
TCTCAGGGAA GTGCTTCCTC CCACAACTCA GCAAGCTGGG GACAAGGAGG AAAGAAGCAA 
ATGAAGTGCT CACTGAAAGG AGGAAACAAT GATTCATGGA TGAATCCTCT TGCCAAACAG 
TTTTCAAATA TGGGATTGCT GAGTCAAACT GAAGATAATC CAGGCAGCAA AATGGATTTG 
TCTGTAGGGA GCCTTCCAGA TAAAAAATTT GACGTGGACA AGCGAGCAAT GAATCTCGGG 
GATTTTAATG ATATCATGAG GAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTC GAGAAGCTGA CTTTGCCTTT CTCCAATCAA 
GATGGGTGCC TTGGGGATGA GGCTCCCTGC TCTCCCTTCT CCCCTTCTCC CAGCTACAAG 
CTGTCTCCCT CTGGTTCCAC ACTACCCAAC GTCAGCCTTG GAGCAATCGG CACAGGGCTC 
AACCCCCAAA ACTTCGCTGC TAGACAAGGT GGCAATCATG GTTTGTTTGG AAATAGCACA 
GCACAATCGA GAGGTATGCA CACACCAGTG CAGCCACTAA ATTCTTCTCC TAATCTCCGG 
GCGCAAGTGC CTCCCCAGTT TATTTCCCCC CAGGTTTCTG CCTCCATGCT CAAGCAGTTT 
CCCAACAGTG GCCTGAATCC AGGTCTTTTC AATGTGGGGC CCCAGTTATC TCCTCAACAG 
ATTGCCATGC TGAGCCAGCT TCCACAAATT CCCCAGTTTC AGTTGGCATG TCAGCTTCTC 
CTGCAGCAGC AGCAACAGCA GCAGTTGCTA CAGAACCAGA GGAAGCTTTC TCAAGCTGTG 
CGCCAGCAGC AAGAGCAGCA GCTGGCTCGA ATGGTGAGCG CTCTCCAGCA ACAGCAGCAA 
CAGCAGCAGC AGCAGCAGCA GAGGCAGCCT GGCATGAAGC ACTCACCCTC TCATCCTGTT 
GGGCCCAAGC CACATCTGGA CAACATGGTA CCCAACGCTC TGAATGTGGG GCTCCCAGAC 
CTTCAAACCA AAGGGCCAAT ACCTGGATAT GGTTCTGGCT TCAGCTCTGG CGGCATGGAC 
TATGGCATGG TTGGTGGGAA GGAGGCTGGA ACAGAGTCTC GCTTTAAACA GTGGACCTCC 
ATGATGGAGG GACTGCCCTC TGTAGCCACA CAGGAAGCCA ATATGCACAA AAATGGCGCT 
ATTGTGGCCC CTGGTAAGAC TCGAGGAGGG TCACCGTACA ACCAGTTTGA TATCATCCCG 
AGTGACACAC TGGGTGGCCA TACGGGTCCT GCTGGTGATA GCTGGTTACC TGCCAAATCT 
CCACCAACAA ATAAAATCGG AAGTAAATCC AGCAATGCCA GTTGGCCTCC AGAATTCCAA 
CCAGGAGTGC CATGGAAAGG TATCCAAAAC ATTGACCCTG AATCTGACCC CTATGTCACC 
CCAGGAAGTG TACTGGGGGG TACAGCCACA TCTCCCATTG TAGATACTGA CCACCAACTT 
CTGCGGGATA ACACCACAGG GTCTAATTCT TCCCTCAACA CCTCGCTGCC TTCACCTGGT 
GCCTGGCCCT ACAGTGCCTC TGACAACTCC TTTACCAACG TTCATAGCAC TTCAGCAAAG 
TTCCCTGATT ACAAATCAAC ATGGTCCCCA GATCCCATAG GACACAACCC CACTCATCTC 
TCCAACAAGA TGTGGAAAAA CCATATTTCC TCAAGGAACA CTACACCGCT GCCCCGCCCA 
CCTCCCGGTC TGACCAACCC CAAACCATCA TCTCCCTGGA GCAGCACAGC ACCCCGATCA 
GTCAGGGGGT GGGGGACACA GGACTCACGG CTTGCCTCGG CCTCTACCTG GAGTGATGGT 
GGTTCAGTTC GTCCCAGTTA CTGGCTGGTT CTTCACAATC TCACTCCACA GATTGATGGG 
TCAACGTTGA GAACCATCTG CATGCAGCAT GGCCCACTGC TGACATTCCA TCTGAACCTA 
ACCCAGGGCA CTGCCCTGAT CCGATACAGC ACCAAACAGG AGGCGGCCAA GGCCCAAACT 
GCACTGCACA TGTGTGTGTT GGGAAACACT ACCATCCTGG CTGAGTTTGC CACCGATGAT 
GAAGTTAGCC GCTTTCTGGC ACAAGCTCAG CCCCCTACAC CTGCAGCAAC CCCAAGTGCA 
CCCGCCGCAG GTTGGCAGTC GCTGGAGACC GGCCAGACCC AGTCAGATCC CGTCGGACCT 
GCTTTGAATC TTTTTGGTGG GTCCACAGGG CTCGGGCAAT GGAGCAGCAG TGCTGGTGGC 
AGCAGCGGGG CCGATCTTGC TGGCGCTTCG TTGTGGGGGC CCCCAAACTA TTCTTCTAGC 
TTGTGGGGAG TCCCAGCTGT GGAAGATCCC CATAGGATGG GCAGCCCTGC TCCCTTACTA 
CCTGGTGACC TTCTGGGAGG AGGGTCGGAT TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_005625852.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005625909.2 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

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CloneID	OCe38894
Clone ID Related Accession (Same CDS sequence)	XM_005625852.2
Accession Version	XM_005625852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCACC TTCCTCTTTC CATCAATCCA TGTCCCACCA TTTTCCATTC CATAACAGAA 
GTCAGCTTCA TGAGAATACT CTTTGCTTCT ACTTTAATCT CTTCCAGAGA GAAGGAGCAA 
GAAAGGGAAG AACAGTTAAT GGAAGACAAG AAAAGGAAGA AAGAGGATAA AAAGAAAAAA 
GAAGCCACTC AGAAGGTCAC GGAACAAAAA ACCAAAGTGC CCGAAGTGAC GAAACCAAGT 
TTAAGCCAAC CAACGGCCGC CAGCCCAATT GGCAGCTCTC CATCGCCACC AGTCAATGGT 
GGCAACAATG CCAAAAGGGT GGCAGTGCCG AACGGACAAC CGCCAAGCGC CGCCCGCTAC 
ATGCCTCGGG AGGTGCCGCC GCGATTCCGT TGCCAGCAGG ACCACAAAGT GTTACTAAAA 
CGTGGGCAGC CCCCTCCGCC GTCCTGCATG CTCCTTGGGG GCGGAGCAGG GCCTCCTCCC 
TCCACAGCAC CTGGAGCAAA CTCAAACAAC GCACAAGTGA CAGGAACGCT GCTGCAGAGT 
GACAGTGGGA CTGCACCAGA GTCAACCCTT GGAGGTGCTG CTGCTTCAAA TTATGCAAAT 
TCCACTTGGG GCCCGGGAGC CTCCTCCAAC AACGGCGCCT CCCCCAACCC AATTCACACC 
TGGGACAAGG TGATTGTAGA CGGGTCTGAC GTGGAAGAGT GGCCTTGTAT TGCCAGCAAA 
GACACTGAGT CTTCTTCCGA AAACACCACC GATAACAACA GTGCCTCGAA CCCTGGCTCT 
GAGAAGAGCA CTCTGCCAGG AAGCACCACT AGTAACAAAG GAAAAGGGAG CCAGTGCCAG 
TCTGCAAGTT CTGGGAACGA ATGTAATCTT GGGGTCTGGA AATCTGACCC TAAGACTAAA 
TCTGTTCAAT CTTCCAACTC TACAACAGAG AGCAACAATG GACTAGGAAA TTGGAGGAAT 
GTGAGTGGTC AGGATAGAAT TGGACCTGGC TCTGGCTTCA GCAACTTTAA CCCAAATAGC 
AACCCATCTG CCTGGCCAGC GCTGGTCCAA GAAGGAAATT CTAGGAAAGG GGCATTGGAA 
ACGGATACTA GTAATTCCAG TGCACAGGTT AGCACAGTAG GTCAGGCATC CAGGGAACAG 
CAGTCAAAGA TGGAAAATGC GGGTGTTAAT TTTGTTGTCT CTGGCAGAGA ACAGGCTCAG 
ATTCATAACA CTGATGGACC AAAAAATGGA AACACTAACT CCTTGAACTT AAGTTCACCA 
AACCCCATGG AGAATAAGGG AATGCCCTTT GGAATGGGCT TGGGGAACGC CTCCAGGAGC 
ACTGATGCCC CTTCACAAAG CACTGGAGAT CGAAAGACTG GGAGTGTTGG ATCTTGGGGT 
GCAGCTAGGG GGCCTTCTGG AACTGACACA GCCTCTGGAC AAAGCAATTC TGGAAACAAT 
GGGAACAATG GAAAAGATAG AGAGGACTCC TGGAAAGGAG CTTCTGTTCA GAAATCATCT 
GGGTCAAAAA ATGACTCTTG GGACAATAAC AGGTCTACGG GTGGGTCCTG GAACTTTGGC 
CCTCAGGACT CTAATGACAA CAAATGGGGT GAAGGGAACA AAATGACATC TGGGGTCTCT 
CAGGGAGAAT GGAAACAGCC GACTGGGTCT GATGAGTTGA AAATTGGAGA ATGGAGTGGT 
CCAAACCAAC CAAATTCTAG CACTGGAGCA TGGGACAATC AAAAGGGCCA CCCCCTCCCT 
GAAAACCAAG GCAATGCCCA GGCTCCCTGT TGGGGAAGAT CTTCCAGCTC CACAGGAAGT 
GAAGTTGGAG GTCAAAGCAC TGGAAGCAAC CACAAAGCAG GAAGTAGTGA CAGTCATAAT 
TCTGGCCGTC GGTCATACCG GCCCACACAT CCTGATTGTC AGGCTGTCTT ACAGACTCTT 
TTGAGCCGAA CTGATTTGGA CCCCAGGGTG CTCTCAAACA CTGGCTGGGG CCAAACTCAA 
ATTAAGCAGG ACACTGTGTG GGATATTGAA GAGGTGCCAA GGCCTGAGGG GAAATCGGAC 
AAAGGAACTG AGGGGTGGGA GAGCGCTGCC ACACAGACCA AGAACTCAGG GGGCTGGGGA 
GATGCACCCA GCCAAAGCAA TCAAATGAAG TCTGGATGGG GGGAGCTCTC AGCCTCTACA 
GAGTGGAAAG ACCCCAAGAA CACAGGAGGC TGGAATGACT ATAAGAACAA CAACTCTTCC 
AACTGGGGAG GCGGACGACC AGATGAAAAG ACATCTTCCT CTTGGAATGA GAACTCCAGC 
AAGGATCAGG GGTGGGGAGG GGGACGCCAG CCCAATCAAG GATGGACTTC TGGGAAGAAT 
GGTTGGGGAG AGGAAGTTGA TCAGGCGAAA AACAGCAGTT GGGAAAGTTC TGCAAGCAAA 
CCTGTGTCTG GATGGGGAGA AGGAGGGCAG AATGAAATTG GAACTTGGGG GAACAGCGGG 
AATGCAAGCC TAGCTTCCAA AGGTGGGTGG GAAGATTGCA AAAGGTCTCC AGCGTGGACT 
GAGACTGGCA GACAGCCCAA TTCCTGGCAT AAACAGCACC AACAGCAGCA GCAGCCACAG 
CAGCCACCGC CACCACAACC AGAGGCTTCT GGTTCATGGG GAGGCCCCCC ACCACCACCT 
CCAGGCAACG GACGACCTTC CAATTCCAAC TGGAGCAGTG GGCCACAGCC AGTAACCCCT 
AAGGACGAGG AACCCAGTGG TTGGGAAGAG CCATCCCCAC AGTCAATTAG TCGGAAAATG 
GACATCGATG ATGGCACTTC AGCATGGGGA GACCCTAACA GCTATAACTA CAAGAATGTG 
AATCTGTGGG ATAAGAATTC CCAAGGGGGC CCAGCACCTC GAGAACCAAA CCTGCCTACC 
CCGATGACCA GTAAATCAGC ATCAGTCTGG AGCAAAAGCA CACCACCTGC TCCAGATAAT 
GGTACTTCAG CTTGGGGTGA GCCAAACGAA AGCAGTCCTG GGTGGGGCGA AATGGATGAC 
ACGGGAGCAT CGACCACGGG CTGGGGGAAT GCACCCTCCA ACGCTCCAAA TGCCATGAAA 
CCTAATTCCA AATCTATGCA AGACGGCTGG GGGGAGAGTG ACGGTCCAGT GACGGGAACT 
CGCCATCCCA GCTGGGAGGA GGAGGATGAT GGAGGAGTCT GGAACACCGC CGGCTCTCAG 
GGAAGTGCTT CCTCCCACAA CTCAGCAAGC TGGGGACAAG GAGGAAAGAA GCAAATGAAG 
TGCTCACTGA AAGGAGGAAA CAATGATTCA TGGATGAATC CTCTTGCCAA ACAGTTTTCA 
AATATGGGAT TGCTGAGTCA AACTGAAGAT AATCCAGGCA GCAAAATGGA TTTGTCTGTA 
GGGAGCCTTC CAGATAAAAA ATTTGACGTG GACAAGCGAG CAATGAATCT CGGGGATTTT 
AATGATATCA TGAGGAAGGA TCGATCTGGG TTCCGTCCAC CTAATTCCAA AGACATGGGA 
ACCACAGATA GTGGGCCTTA TTTCGAGAAG CTGACTTTGC CTTTCTCCAA TCAAGATGGG 
TGCCTTGGGG ATGAGGCTCC CTGCTCTCCC TTCTCCCCTT CTCCCAGCTA CAAGCTGTCT 
CCCTCTGGTT CCACACTACC CAACGTCAGC CTTGGAGCAA TCGGCACAGG GCTCAACCCC 
CAAAACTTCG CTGCTAGACA AGGTGGCAAT CATGGTTTGT TTGGAAATAG CACAGCACAA 
TCGAGAGGTA TGCACACACC AGTGCAGCCA CTAAATTCTT CTCCTAATCT CCGGGCGCAA 
GTGCCTCCCC AGTTTATTTC CCCCCAGGTT TCTGCCTCCA TGCTCAAGCA GTTTCCCAAC 
AGTGGCCTGA ATCCAGGTCT TTTCAATGTG GGGCCCCAGT TATCTCCTCA ACAGATTGCC 
ATGCTGAGCC AGCTTCCACA AATTCCCCAG TTTCAGTTGG CATGTCAGCT TCTCCTGCAG 
CAGCAGCAAC AGCAGCAGTT GCTACAGAAC CAGAGGAAGC TTTCTCAAGC TGTGCGCCAG 
CAGCAAGAGC AGCAGCTGGC TCGAATGGTG AGCGCTCTCC AGCAACAGCA GCAACAGCAG 
CAGCAGCAGC AGCAGAGGCA GCCTGGCATG AAGCACTCAC CCTCTCATCC TGTTGGGCCC 
AAGCCACATC TGGACAACAT GGTACCCAAC GCTCTGAATG TGGGGCTCCC AGACCTTCAA 
ACCAAAGGGC CAATACCTGG ATATGGTTCT GGCTTCAGCT CTGGCGGCAT GGACTATGGC 
ATGGTTGGTG GGAAGGAGGC TGGAACAGAG TCTCGCTTTA AACAGTGGAC CTCCATGATG 
GAGGGACTGC CCTCTGTAGC CACACAGGAA GCCAATATGC ACAAAAATGG CGCTATTGTG 
GCCCCTGGTA AGACTCGAGG AGGGTCACCG TACAACCAGT TTGATATCAT CCCGAGTGAC 
ACACTGGGTG GCCATACGGG TCCTGCTGGT GATAGCTGGT TACCTGCCAA ATCTCCACCA 
ACAAATAAAA TCGGAAGTAA ATCCAGCAAT GCCAGTTGGC CTCCAGAATT CCAACCAGGA 
GTGCCATGGA AAGGTATCCA AAACATTGAC CCTGAATCTG ACCCCTATGT CACCCCAGGA 
AGTGTACTGG GGGGTACAGC CACATCTCCC ATTGTAGATA CTGACCACCA ACTTCTGCGG 
GATAACACCA CAGGGTCTAA TTCTTCCCTC AACACCTCGC TGCCTTCACC TGGTGCCTGG 
CCCTACAGTG CCTCTGACAA CTCCTTTACC AACGTTCATA GCACTTCAGC AAAGTTCCCT 
GATTACAAAT CAACATGGTC CCCAGATCCC ATAGGACACA ACCCCACTCA TCTCTCCAAC 
AAGATGTGGA AAAACCATAT TTCCTCAAGG AACACTACAC CGCTGCCCCG CCCACCTCCC 
GGTCTGACCA ACCCCAAACC ATCATCTCCC TGGAGCAGCA CAGCACCCCG ATCAGTCAGG 
GGGTGGGGGA CACAGGACTC ACGGCTTGCC TCGGCCTCTA CCTGGAGTGA TGGTGGTTCA 
GTTCGTCCCA GTTACTGGCT GGTTCTTCAC AATCTCACTC CACAGATTGA TGGGTCAACG 
TTGAGAACCA TCTGCATGCA GCATGGCCCA CTGCTGACAT TCCATCTGAA CCTAACCCAG 
GGCACTGCCC TGATCCGATA CAGCACCAAA CAGGAGGCGG CCAAGGCCCA AACTGCACTG 
CACATGTGTG TGTTGGGAAA CACTACCATC CTGGCTGAGT TTGCCACCGA TGATGAAGTT 
AGCCGCTTTC TGGCACAAGC TCAGCCCCCT ACACCTGCAG CAACCCCAAG TGCACCCGCC 
GCAGGTTGGC AGTCGCTGGA GACCGGCCAG ACCCAGTCAG ATCCCGTCGG ACCTGCTTTG 
AATCTTTTTG GTGGGTCCAC AGGGCTCGGG CAATGGAGCA GCAGTGCTGG TGGCAGCAGC 
GGGGCCGATC TTGCTGGCGC TTCGTTGTGG GGGCCCCCAA ACTATTCTTC TAGCTTGTGG 
GGAGTCCCAG CTGTGGAAGA TCCCCATAGG ATGGGCAGCC CTGCTCCCTT ACTACCTGGT 
GACCTTCTGG GAGGAGGGTC GGATTCAATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005625909.2
CDS	571..6183
Translation	MTHLPLSINPCPTIFHSITEVSFMRILFASTLISSREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_005625852.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625852.2

ORF Insert Sequence:

ATGACCCACC TTCCTCTTTC CATCAATCCA TGTCCCACCA TTTTCCATTC CATAACAGAA 
GTCAGCTTCA TGAGAATACT CTTTGCTTCT ACTTTAATCT CTTCCAGAGA GAAGGAGCAA 
GAAAGGGAAG AACAGTTAAT GGAAGACAAG AAAAGGAAGA AAGAGGATAA AAAGAAAAAA 
GAAGCCACTC AGAAGGTCAC GGAACAAAAA ACCAAAGTGC CCGAAGTGAC GAAACCAAGT 
TTAAGCCAAC CAACGGCCGC CAGCCCAATT GGCAGCTCTC CATCGCCACC AGTCAATGGT 
GGCAACAATG CCAAAAGGGT GGCAGTGCCG AACGGACAAC CGCCAAGCGC CGCCCGCTAC 
ATGCCTCGGG AGGTGCCGCC GCGATTCCGT TGCCAGCAGG ACCACAAAGT GTTACTAAAA 
CGTGGGCAGC CCCCTCCGCC GTCCTGCATG CTCCTTGGGG GCGGAGCAGG GCCTCCTCCC 
TCCACAGCAC CTGGAGCAAA CTCAAACAAC GCACAAGTGA CAGGAACGCT GCTGCAGAGT 
GACAGTGGGA CTGCACCAGA GTCAACCCTT GGAGGTGCTG CTGCTTCAAA TTATGCAAAT 
TCCACTTGGG GCCCGGGAGC CTCCTCCAAC AACGGCGCCT CCCCCAACCC AATTCACACC 
TGGGACAAGG TGATTGTAGA CGGGTCTGAC GTGGAAGAGT GGCCTTGTAT TGCCAGCAAA 
GACACTGAGT CTTCTTCCGA AAACACCACC GATAACAACA GTGCCTCGAA CCCTGGCTCT 
GAGAAGAGCA CTCTGCCAGG AAGCACCACT AGTAACAAAG GAAAAGGGAG CCAGTGCCAG 
TCTGCAAGTT CTGGGAACGA ATGTAATCTT GGGGTCTGGA AATCTGACCC TAAGACTAAA 
TCTGTTCAAT CTTCCAACTC TACAACAGAG AGCAACAATG GACTAGGAAA TTGGAGGAAT 
GTGAGTGGTC AGGATAGAAT TGGACCTGGC TCTGGCTTCA GCAACTTTAA CCCAAATAGC 
AACCCATCTG CCTGGCCAGC GCTGGTCCAA GAAGGAAATT CTAGGAAAGG GGCATTGGAA 
ACGGATACTA GTAATTCCAG TGCACAGGTT AGCACAGTAG GTCAGGCATC CAGGGAACAG 
CAGTCAAAGA TGGAAAATGC GGGTGTTAAT TTTGTTGTCT CTGGCAGAGA ACAGGCTCAG 
ATTCATAACA CTGATGGACC AAAAAATGGA AACACTAACT CCTTGAACTT AAGTTCACCA 
AACCCCATGG AGAATAAGGG AATGCCCTTT GGAATGGGCT TGGGGAACGC CTCCAGGAGC 
ACTGATGCCC CTTCACAAAG CACTGGAGAT CGAAAGACTG GGAGTGTTGG ATCTTGGGGT 
GCAGCTAGGG GGCCTTCTGG AACTGACACA GCCTCTGGAC AAAGCAATTC TGGAAACAAT 
GGGAACAATG GAAAAGATAG AGAGGACTCC TGGAAAGGAG CTTCTGTTCA GAAATCATCT 
GGGTCAAAAA ATGACTCTTG GGACAATAAC AGGTCTACGG GTGGGTCCTG GAACTTTGGC 
CCTCAGGACT CTAATGACAA CAAATGGGGT GAAGGGAACA AAATGACATC TGGGGTCTCT 
CAGGGAGAAT GGAAACAGCC GACTGGGTCT GATGAGTTGA AAATTGGAGA ATGGAGTGGT 
CCAAACCAAC CAAATTCTAG CACTGGAGCA TGGGACAATC AAAAGGGCCA CCCCCTCCCT 
GAAAACCAAG GCAATGCCCA GGCTCCCTGT TGGGGAAGAT CTTCCAGCTC CACAGGAAGT 
GAAGTTGGAG GTCAAAGCAC TGGAAGCAAC CACAAAGCAG GAAGTAGTGA CAGTCATAAT 
TCTGGCCGTC GGTCATACCG GCCCACACAT CCTGATTGTC AGGCTGTCTT ACAGACTCTT 
TTGAGCCGAA CTGATTTGGA CCCCAGGGTG CTCTCAAACA CTGGCTGGGG CCAAACTCAA 
ATTAAGCAGG ACACTGTGTG GGATATTGAA GAGGTGCCAA GGCCTGAGGG GAAATCGGAC 
AAAGGAACTG AGGGGTGGGA GAGCGCTGCC ACACAGACCA AGAACTCAGG GGGCTGGGGA 
GATGCACCCA GCCAAAGCAA TCAAATGAAG TCTGGATGGG GGGAGCTCTC AGCCTCTACA 
GAGTGGAAAG ACCCCAAGAA CACAGGAGGC TGGAATGACT ATAAGAACAA CAACTCTTCC 
AACTGGGGAG GCGGACGACC AGATGAAAAG ACATCTTCCT CTTGGAATGA GAACTCCAGC 
AAGGATCAGG GGTGGGGAGG GGGACGCCAG CCCAATCAAG GATGGACTTC TGGGAAGAAT 
GGTTGGGGAG AGGAAGTTGA TCAGGCGAAA AACAGCAGTT GGGAAAGTTC TGCAAGCAAA 
CCTGTGTCTG GATGGGGAGA AGGAGGGCAG AATGAAATTG GAACTTGGGG GAACAGCGGG 
AATGCAAGCC TAGCTTCCAA AGGTGGGTGG GAAGATTGCA AAAGGTCTCC AGCGTGGACT 
GAGACTGGCA GACAGCCCAA TTCCTGGCAT AAACAGCACC AACAGCAGCA GCAGCCACAG 
CAGCCACCGC CACCACAACC AGAGGCTTCT GGTTCATGGG GAGGCCCCCC ACCACCACCT 
CCAGGCAACG GACGACCTTC CAATTCCAAC TGGAGCAGTG GGCCACAGCC AGTAACCCCT 
AAGGACGAGG AACCCAGTGG TTGGGAAGAG CCATCCCCAC AGTCAATTAG TCGGAAAATG 
GACATCGATG ATGGCACTTC AGCATGGGGA GACCCTAACA GCTATAACTA CAAGAATGTG 
AATCTGTGGG ATAAGAATTC CCAAGGGGGC CCAGCACCTC GAGAACCAAA CCTGCCTACC 
CCGATGACCA GTAAATCAGC ATCAGTCTGG AGCAAAAGCA CACCACCTGC TCCAGATAAT 
GGTACTTCAG CTTGGGGTGA GCCAAACGAA AGCAGTCCTG GGTGGGGCGA AATGGATGAC 
ACGGGAGCAT CGACCACGGG CTGGGGGAAT GCACCCTCCA ACGCTCCAAA TGCCATGAAA 
CCTAATTCCA AATCTATGCA AGACGGCTGG GGGGAGAGTG ACGGTCCAGT GACGGGAACT 
CGCCATCCCA GCTGGGAGGA GGAGGATGAT GGAGGAGTCT GGAACACCGC CGGCTCTCAG 
GGAAGTGCTT CCTCCCACAA CTCAGCAAGC TGGGGACAAG GAGGAAAGAA GCAAATGAAG 
TGCTCACTGA AAGGAGGAAA CAATGATTCA TGGATGAATC CTCTTGCCAA ACAGTTTTCA 
AATATGGGAT TGCTGAGTCA AACTGAAGAT AATCCAGGCA GCAAAATGGA TTTGTCTGTA 
GGGAGCCTTC CAGATAAAAA ATTTGACGTG GACAAGCGAG CAATGAATCT CGGGGATTTT 
AATGATATCA TGAGGAAGGA TCGATCTGGG TTCCGTCCAC CTAATTCCAA AGACATGGGA 
ACCACAGATA GTGGGCCTTA TTTCGAGAAG CTGACTTTGC CTTTCTCCAA TCAAGATGGG 
TGCCTTGGGG ATGAGGCTCC CTGCTCTCCC TTCTCCCCTT CTCCCAGCTA CAAGCTGTCT 
CCCTCTGGTT CCACACTACC CAACGTCAGC CTTGGAGCAA TCGGCACAGG GCTCAACCCC 
CAAAACTTCG CTGCTAGACA AGGTGGCAAT CATGGTTTGT TTGGAAATAG CACAGCACAA 
TCGAGAGGTA TGCACACACC AGTGCAGCCA CTAAATTCTT CTCCTAATCT CCGGGCGCAA 
GTGCCTCCCC AGTTTATTTC CCCCCAGGTT TCTGCCTCCA TGCTCAAGCA GTTTCCCAAC 
AGTGGCCTGA ATCCAGGTCT TTTCAATGTG GGGCCCCAGT TATCTCCTCA ACAGATTGCC 
ATGCTGAGCC AGCTTCCACA AATTCCCCAG TTTCAGTTGG CATGTCAGCT TCTCCTGCAG 
CAGCAGCAAC AGCAGCAGTT GCTACAGAAC CAGAGGAAGC TTTCTCAAGC TGTGCGCCAG 
CAGCAAGAGC AGCAGCTGGC TCGAATGGTG AGCGCTCTCC AGCAACAGCA GCAACAGCAG 
CAGCAGCAGC AGCAGAGGCA GCCTGGCATG AAGCACTCAC CCTCTCATCC TGTTGGGCCC 
AAGCCACATC TGGACAACAT GGTACCCAAC GCTCTGAATG TGGGGCTCCC AGACCTTCAA 
ACCAAAGGGC CAATACCTGG ATATGGTTCT GGCTTCAGCT CTGGCGGCAT GGACTATGGC 
ATGGTTGGTG GGAAGGAGGC TGGAACAGAG TCTCGCTTTA AACAGTGGAC CTCCATGATG 
GAGGGACTGC CCTCTGTAGC CACACAGGAA GCCAATATGC ACAAAAATGG CGCTATTGTG 
GCCCCTGGTA AGACTCGAGG AGGGTCACCG TACAACCAGT TTGATATCAT CCCGAGTGAC 
ACACTGGGTG GCCATACGGG TCCTGCTGGT GATAGCTGGT TACCTGCCAA ATCTCCACCA 
ACAAATAAAA TCGGAAGTAA ATCCAGCAAT GCCAGTTGGC CTCCAGAATT CCAACCAGGA 
GTGCCATGGA AAGGTATCCA AAACATTGAC CCTGAATCTG ACCCCTATGT CACCCCAGGA 
AGTGTACTGG GGGGTACAGC CACATCTCCC ATTGTAGATA CTGACCACCA ACTTCTGCGG 
GATAACACCA CAGGGTCTAA TTCTTCCCTC AACACCTCGC TGCCTTCACC TGGTGCCTGG 
CCCTACAGTG CCTCTGACAA CTCCTTTACC AACGTTCATA GCACTTCAGC AAAGTTCCCT 
GATTACAAAT CAACATGGTC CCCAGATCCC ATAGGACACA ACCCCACTCA TCTCTCCAAC 
AAGATGTGGA AAAACCATAT TTCCTCAAGG AACACTACAC CGCTGCCCCG CCCACCTCCC 
GGTCTGACCA ACCCCAAACC ATCATCTCCC TGGAGCAGCA CAGCACCCCG ATCAGTCAGG 
GGGTGGGGGA CACAGGACTC ACGGCTTGCC TCGGCCTCTA CCTGGAGTGA TGGTGGTTCA 
GTTCGTCCCA GTTACTGGCT GGTTCTTCAC AATCTCACTC CACAGATTGA TGGGTCAACG 
TTGAGAACCA TCTGCATGCA GCATGGCCCA CTGCTGACAT TCCATCTGAA CCTAACCCAG 
GGCACTGCCC TGATCCGATA CAGCACCAAA CAGGAGGCGG CCAAGGCCCA AACTGCACTG 
CACATGTGTG TGTTGGGAAA CACTACCATC CTGGCTGAGT TTGCCACCGA TGATGAAGTT 
AGCCGCTTTC TGGCACAAGC TCAGCCCCCT ACACCTGCAG CAACCCCAAG TGCACCCGCC 
GCAGGTTGGC AGTCGCTGGA GACCGGCCAG ACCCAGTCAG ATCCCGTCGG ACCTGCTTTG 
AATCTTTTTG GTGGGTCCAC AGGGCTCGGG CAATGGAGCA GCAGTGCTGG TGGCAGCAGC 
GGGGCCGATC TTGCTGGCGC TTCGTTGTGG GGGCCCCCAA ACTATTCTTC TAGCTTGTGG 
GGAGTCCCAG CTGTGGAAGA TCCCCATAGG ATGGGCAGCC CTGCTCCCTT ACTACCTGGT 
GACCTTCTGG GAGGAGGGTC GGATTCAATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_014117341.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972816.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

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CloneID	OCe38895
Clone ID Related Accession (Same CDS sequence)	XM_014117341.1
Accession Version	XM_014117341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAA TGCTGGCCCC TATCTTTCTG CAGGGTGCAG TGATCCAGAA TCCAGAAGGG 
CTGTTAGTTG AGAGAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAAAAAGAA GCCACTCAGA AGGTCACGGA ACAAAAAACC 
AAAGTGCCCG AAGTGACGAA ACCAAGTTTA AGCCAACCAA CGGCCGCCAG CCCAATTGGC 
AGCTCTCCAT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CAAGCGCCGC CCGCTACATG CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC 
CAGCAGGACC ACAAAGTGTT ACTAAAACGT GGGCAGCCCC CTCCGCCGTC CTGCATGCTC 
CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC ACAGCACCTG GAGCAAACTC AAACAACGCA 
CAAGTGACAG GAACGCTGCT GCAGAGTGAC AGTGGGACTG CACCAGAGTC AACCCTTGGA 
GGTGCTGCTG CTTCAAATTA TGCAAATTCC ACTTGGGGCC CGGGAGCCTC CTCCAACAAC 
GGCGCCTCCC CCAACCCAAT TCACACCTGG GACAAGGTGA TTGTAGACGG GTCTGACGTG 
GAAGAGTGGC CTTGTATTGC CAGCAAAGAC ACTGAGTCTT CTTCCGAAAA CACCACCGAT 
AACAACAGTG CCTCGAACCC TGGCTCTGAG AAGAGCACTC TGCCAGGAAG CACCACTAGT 
AACAAAGGAA AAGGGAGCCA GTGCCAGTCT GCAAGTTCTG GGAACGAATG TAATCTTGGG 
GTCTGGAAAT CTGACCCTAA GACTAAATCT GTTCAATCTT CCAACTCTAC AACAGAGAGC 
AACAATGGAC TAGGAAATTG GAGGAATGTG AGTGGTCAGG ATAGAATTGG ACCTGGCTCT 
GGCTTCAGCA ACTTTAACCC AAATAGCAAC CCATCTGCCT GGCCAGCGCT GGTCCAAGAA 
GGAAATTCTA GGAAAGGGGC ATTGGAAACG GATACTAGTA ATTCCAGTGC ACAGGTTAGC 
ACAGTAGGTC AGGCATCCAG GGAACAGCAG TCAAAGATGG AAAATGCGGG TGTTAATTTT 
GTTGTCTCTG GCAGAGAACA GGCTCAGATT CATAACACTG ATGGACCAAA AAATGGAAAC 
ACTAACTCCT TGAACTTAAG TTCACCAAAC CCCATGGAGA ATAAGGGAAT GCCCTTTGGA 
ATGGGCTTGG GGAACGCCTC CAGGAGCACT GATGCCCCTT CACAAAGCAC TGGAGATCGA 
AAGACTGGGA GTGTTGGATC TTGGGGTGCA GCTAGGGGGC CTTCTGGAAC TGACACAGCC 
TCTGGACAAA GCAATTCTGG AAACAATGGG AACAATGGAA AAGATAGAGA GGACTCCTGG 
AAAGGAGCTT CTGTTCAGAA ATCATCTGGG TCAAAAAATG ACTCTTGGGA CAATAACAGG 
TCTACGGGTG GGTCCTGGAA CTTTGGCCCT CAGGACTCTA ATGACAACAA ATGGGGTGAA 
GGGAACAAAA TGACATCTGG GGTCTCTCAG GGAGAATGGA AACAGCCGAC TGGGTCTGAT 
GAGTTGAAAA TTGGAGAATG GAGTGGTCCA AACCAACCAA ATTCTAGCAC TGGAGCATGG 
GACAATCAAA AGGGCCACCC CCTCCCTGAA AACCAAGGCA ATGCCCAGGC TCCCTGTTGG 
GGAAGATCTT CCAGCTCCAC AGGAAGTGAA GTTGGAGGTC AAAGCACTGG AAGCAACCAC 
AAAGCAGGAA GTAGTGACAG TCATAATTCT GGCCGTCGGT CATACCGGCC CACACATCCT 
GATTGTCAGG CTGTCTTACA GACTCTTTTG AGCCGAACTG ATTTGGACCC CAGGGTGCTC 
TCAAACACTG GCTGGGGCCA AACTCAAATT AAGCAGGACA CTGTGTGGGA TATTGAAGAG 
GTGCCAAGGC CTGAGGGGAA ATCGGACAAA GGAACTGAGG GGTGGGAGAG CGCTGCCACA 
CAGACCAAGA ACTCAGGGGG CTGGGGAGAT GCACCCAGCC AAAGCAATCA AATGAAGTCT 
GGATGGGGGG AGCTCTCAGC CTCTACAGAG TGGAAAGACC CCAAGAACAC AGGAGGCTGG 
AATGACTATA AGAACAACAA CTCTTCCAAC TGGGGAGGCG GACGACCAGA TGAAAAGACA 
TCTTCCTCTT GGAATGAGAA CTCCAGCAAG GATCAGGGGT GGGGAGGGGG ACGCCAGCCC 
AATCAAGGAT GGACTTCTGG GAAGAATGGT TGGGGAGAGG AAGTTGATCA GGCGAAAAAC 
AGCAGTTGGG AAAGTTCTGC AAGCAAACCT GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT 
GAAATTGGAA CTTGGGGGAA CAGCGGGAAT GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA 
GATTGCAAAA GGTCTCCAGC GTGGACTGAG ACTGGCAGAC AGCCCAATTC CTGGCATAAA 
CAGCACCAAC AGCAGCAGCA GCCACAGCAG CCACCGCCAC CACAACCAGA GGCTTCTGGT 
TCATGGGGAG GCCCCCCACC ACCACCTCCA GGCAACGGAC GACCTTCCAA TTCCAACTGG 
AGCAGTGGGC CACAGCCAGT AACCCCTAAG GACGAGGAAC CCAGTGGTTG GGAAGAGCCA 
TCCCCACAGT CAATTAGTCG GAAAATGGAC ATCGATGATG GCACTTCAGC ATGGGGAGAC 
CCTAACAGCT ATAACTACAA GAATGTGAAT CTGTGGGATA AGAATTCCCA AGGGGGCCCA 
GCACCTCGAG AACCAAACCT GCCTACCCCG ATGACCAGTA AATCAGCATC AGTCTGGAGC 
AAAAGCACAC CACCTGCTCC AGATAATGGT ACTTCAGCTT GGGGTGAGCC AAACGAAAGC 
AGTCCTGGGT GGGGCGAAAT GGATGACACG GGAGCATCGA CCACGGGCTG GGGGAATGCA 
CCCTCCAACG CTCCAAATGC CATGAAACCT AATTCCAAAT CTATGCAAGA CGGCTGGGGG 
GAGAGTGACG GTCCAGTGAC GGGAACTCGC CATCCCAGCT GGGAGGAGGA GGATGATGGA 
GGAGTCTGGA ACACCGCCGG CTCTCAGGGA AGTGCTTCCT CCCACAACTC AGCAAGCTGG 
GGACAAGGAG GAAAGAAGCA AATGAAGTGC TCACTGAAAG GAGGAAACAA TGATTCATGG 
ATGAATCCTC TTGCCAAACA GTTTTCAAAT ATGGGATTGC TGAGTCAAAC TGAAGATAAT 
CCAGGCAGCA AAATGGATTT GTCTGTAGGG AGCCTTCCAG ATAAAAAATT TGACGTGGAC 
AAGCGAGCAA TGAATCTCGG GGATTTTAAT GATATCATGA GGAAGGATCG ATCTGGGTTC 
CGTCCACCTA ATTCCAAAGA CATGGGAACC ACAGATAGTG GGCCTTATTT CGAGAAGCTG 
ACTTTGCCTT TCTCCAATCA AGATGGGTGC CTTGGGGATG AGGCTCCCTG CTCTCCCTTC 
TCCCCTTCTC CCAGCTACAA GCTGTCTCCC TCTGGTTCCA CACTACCCAA CGTCAGCCTT 
GGAGCAATCG GCACAGGGCT CAACCCCCAA AACTTCGCTG CTAGACAAGG TGGCAATCAT 
GGTTTGTTTG GAAATAGCAC AGCACAATCG AGAGGTATGC ACACACCAGT GCAGCCACTA 
AATTCTTCTC CTAATCTCCG GGCGCAAGTG CCTCCCCAGT TTATTTCCCC CCAGGTTTCT 
GCCTCCATGC TCAAGCAGTT TCCCAACAGT GGCCTGAATC CAGGTCTTTT CAATGTGGGG 
CCCCAGTTAT CTCCTCAACA GATTGCCATG CTGAGCCAGC TTCCACAAAT TCCCCAGTTT 
CAGTTGGCAT GTCAGCTTCT CCTGCAGCAG CAGCAACAGC AGCAGTTGCT ACAGAACCAG 
AGGAAGCTTT CTCAAGCTGT GCGCCAGCAG CAAGAGCAGC AGCTGGCTCG AATGGTGAGC 
GCTCTCCAGC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AGAGGCAGCC TGGCATGAAG 
CACTCACCCT CTCATCCTGT TGGGCCCAAG CCACATCTGG ACAACATGGT ACCCAACGCT 
CTGAATGTGG GGCTCCCAGA CCTTCAAACC AAAGGGCCAA TACCTGGATA TGGTTCTGGC 
TTCAGCTCTG GCGGCATGGA CTATGGCATG GTTGGTGGGA AGGAGGCTGG AACAGAGTCT 
CGCTTTAAAC AGTGGACCTC CATGATGGAG GGACTGCCCT CTGTAGCCAC ACAGGAAGCC 
AATATGCACA AAAATGGCGC TATTGTGGCC CCTGGTAAGA CTCGAGGAGG GTCACCGTAC 
AACCAGTTTG ATATCATCCC GAGTGACACA CTGGGTGGCC ATACGGGTCC TGCTGGTGAT 
AGCTGGTTAC CTGCCAAATC TCCACCAACA AATAAAATCG GAAGTAAATC CAGCAATGCC 
AGTTGGCCTC CAGAATTCCA ACCAGGAGTG CCATGGAAAG GTATCCAAAA CATTGACCCT 
GAATCTGACC CCTATGTCAC CCCAGGAAGT GTACTGGGGG GTACAGCCAC ATCTCCCATT 
GTAGATACTG ACCACCAACT TCTGCGGGAT AACACCACAG GGTCTAATTC TTCCCTCAAC 
ACCTCGCTGC CTTCACCTGG TGCCTGGCCC TACAGTGCCT CTGACAACTC CTTTACCAAC 
GTTCATAGCA CTTCAGCAAA GTTCCCTGAT TACAAATCAA CATGGTCCCC AGATCCCATA 
GGACACAACC CCACTCATCT CTCCAACAAG ATGTGGAAAA ACCATATTTC CTCAAGGAAC 
ACTACACCGC TGCCCCGCCC ACCTCCCGGT CTGACCAACC CCAAACCATC ATCTCCCTGG 
AGCAGCACAG CACCCCGATC AGTCAGGGGG TGGGGGACAC AGGACTCACG GCTTGCCTCG 
GCCTCTACCT GGAGTGATGG TGGTTCAGTT CGTCCCAGTT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATTGATGG GTCAACGTTG AGAACCATCT GCATGCAGCA TGGCCCACTG 
CTGACATTCC ATCTGAACCT AACCCAGGGC ACTGCCCTGA TCCGATACAG CACCAAACAG 
GAGGCGGCCA AGGCCCAAAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTG 
GCTGAGTTTG CCACCGATGA TGAAGTTAGC CGCTTTCTGG CACAAGCTCA GCCCCCTACA 
CCTGCAGCAA CCCCAAGTGC ACCCGCCGCA GGTTGGCAGT CGCTGGAGAC CGGCCAGACC 
CAGTCAGATC CCGTCGGACC TGCTTTGAAT CTTTTTGGTG GGTCCACAGG GCTCGGGCAA 
TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG GCCGATCTTG CTGGCGCTTC GTTGTGGGGG 
CCCCCAAACT ATTCTTCTAG CTTGTGGGGA GTCCCAGCTG TGGAAGATCC CCATAGGATG 
GGCAGCCCTG CTCCCTTACT ACCTGGTGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972816.1
CDS	119..5698
Translation	MLKMLAPIFLQGAVIQNPEGLLVEREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_014117341.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117341.1

ORF Insert Sequence:

ATGCTGAAAA TGCTGGCCCC TATCTTTCTG CAGGGTGCAG TGATCCAGAA TCCAGAAGGG 
CTGTTAGTTG AGAGAGAGAA GGAGCAAGAA AGGGAAGAAC AGTTAATGGA AGACAAGAAA 
AGGAAGAAAG AGGATAAAAA GAAAAAAGAA GCCACTCAGA AGGTCACGGA ACAAAAAACC 
AAAGTGCCCG AAGTGACGAA ACCAAGTTTA AGCCAACCAA CGGCCGCCAG CCCAATTGGC 
AGCTCTCCAT CGCCACCAGT CAATGGTGGC AACAATGCCA AAAGGGTGGC AGTGCCGAAC 
GGACAACCGC CAAGCGCCGC CCGCTACATG CCTCGGGAGG TGCCGCCGCG ATTCCGTTGC 
CAGCAGGACC ACAAAGTGTT ACTAAAACGT GGGCAGCCCC CTCCGCCGTC CTGCATGCTC 
CTTGGGGGCG GAGCAGGGCC TCCTCCCTCC ACAGCACCTG GAGCAAACTC AAACAACGCA 
CAAGTGACAG GAACGCTGCT GCAGAGTGAC AGTGGGACTG CACCAGAGTC AACCCTTGGA 
GGTGCTGCTG CTTCAAATTA TGCAAATTCC ACTTGGGGCC CGGGAGCCTC CTCCAACAAC 
GGCGCCTCCC CCAACCCAAT TCACACCTGG GACAAGGTGA TTGTAGACGG GTCTGACGTG 
GAAGAGTGGC CTTGTATTGC CAGCAAAGAC ACTGAGTCTT CTTCCGAAAA CACCACCGAT 
AACAACAGTG CCTCGAACCC TGGCTCTGAG AAGAGCACTC TGCCAGGAAG CACCACTAGT 
AACAAAGGAA AAGGGAGCCA GTGCCAGTCT GCAAGTTCTG GGAACGAATG TAATCTTGGG 
GTCTGGAAAT CTGACCCTAA GACTAAATCT GTTCAATCTT CCAACTCTAC AACAGAGAGC 
AACAATGGAC TAGGAAATTG GAGGAATGTG AGTGGTCAGG ATAGAATTGG ACCTGGCTCT 
GGCTTCAGCA ACTTTAACCC AAATAGCAAC CCATCTGCCT GGCCAGCGCT GGTCCAAGAA 
GGAAATTCTA GGAAAGGGGC ATTGGAAACG GATACTAGTA ATTCCAGTGC ACAGGTTAGC 
ACAGTAGGTC AGGCATCCAG GGAACAGCAG TCAAAGATGG AAAATGCGGG TGTTAATTTT 
GTTGTCTCTG GCAGAGAACA GGCTCAGATT CATAACACTG ATGGACCAAA AAATGGAAAC 
ACTAACTCCT TGAACTTAAG TTCACCAAAC CCCATGGAGA ATAAGGGAAT GCCCTTTGGA 
ATGGGCTTGG GGAACGCCTC CAGGAGCACT GATGCCCCTT CACAAAGCAC TGGAGATCGA 
AAGACTGGGA GTGTTGGATC TTGGGGTGCA GCTAGGGGGC CTTCTGGAAC TGACACAGCC 
TCTGGACAAA GCAATTCTGG AAACAATGGG AACAATGGAA AAGATAGAGA GGACTCCTGG 
AAAGGAGCTT CTGTTCAGAA ATCATCTGGG TCAAAAAATG ACTCTTGGGA CAATAACAGG 
TCTACGGGTG GGTCCTGGAA CTTTGGCCCT CAGGACTCTA ATGACAACAA ATGGGGTGAA 
GGGAACAAAA TGACATCTGG GGTCTCTCAG GGAGAATGGA AACAGCCGAC TGGGTCTGAT 
GAGTTGAAAA TTGGAGAATG GAGTGGTCCA AACCAACCAA ATTCTAGCAC TGGAGCATGG 
GACAATCAAA AGGGCCACCC CCTCCCTGAA AACCAAGGCA ATGCCCAGGC TCCCTGTTGG 
GGAAGATCTT CCAGCTCCAC AGGAAGTGAA GTTGGAGGTC AAAGCACTGG AAGCAACCAC 
AAAGCAGGAA GTAGTGACAG TCATAATTCT GGCCGTCGGT CATACCGGCC CACACATCCT 
GATTGTCAGG CTGTCTTACA GACTCTTTTG AGCCGAACTG ATTTGGACCC CAGGGTGCTC 
TCAAACACTG GCTGGGGCCA AACTCAAATT AAGCAGGACA CTGTGTGGGA TATTGAAGAG 
GTGCCAAGGC CTGAGGGGAA ATCGGACAAA GGAACTGAGG GGTGGGAGAG CGCTGCCACA 
CAGACCAAGA ACTCAGGGGG CTGGGGAGAT GCACCCAGCC AAAGCAATCA AATGAAGTCT 
GGATGGGGGG AGCTCTCAGC CTCTACAGAG TGGAAAGACC CCAAGAACAC AGGAGGCTGG 
AATGACTATA AGAACAACAA CTCTTCCAAC TGGGGAGGCG GACGACCAGA TGAAAAGACA 
TCTTCCTCTT GGAATGAGAA CTCCAGCAAG GATCAGGGGT GGGGAGGGGG ACGCCAGCCC 
AATCAAGGAT GGACTTCTGG GAAGAATGGT TGGGGAGAGG AAGTTGATCA GGCGAAAAAC 
AGCAGTTGGG AAAGTTCTGC AAGCAAACCT GTGTCTGGAT GGGGAGAAGG AGGGCAGAAT 
GAAATTGGAA CTTGGGGGAA CAGCGGGAAT GCAAGCCTAG CTTCCAAAGG TGGGTGGGAA 
GATTGCAAAA GGTCTCCAGC GTGGACTGAG ACTGGCAGAC AGCCCAATTC CTGGCATAAA 
CAGCACCAAC AGCAGCAGCA GCCACAGCAG CCACCGCCAC CACAACCAGA GGCTTCTGGT 
TCATGGGGAG GCCCCCCACC ACCACCTCCA GGCAACGGAC GACCTTCCAA TTCCAACTGG 
AGCAGTGGGC CACAGCCAGT AACCCCTAAG GACGAGGAAC CCAGTGGTTG GGAAGAGCCA 
TCCCCACAGT CAATTAGTCG GAAAATGGAC ATCGATGATG GCACTTCAGC ATGGGGAGAC 
CCTAACAGCT ATAACTACAA GAATGTGAAT CTGTGGGATA AGAATTCCCA AGGGGGCCCA 
GCACCTCGAG AACCAAACCT GCCTACCCCG ATGACCAGTA AATCAGCATC AGTCTGGAGC 
AAAAGCACAC CACCTGCTCC AGATAATGGT ACTTCAGCTT GGGGTGAGCC AAACGAAAGC 
AGTCCTGGGT GGGGCGAAAT GGATGACACG GGAGCATCGA CCACGGGCTG GGGGAATGCA 
CCCTCCAACG CTCCAAATGC CATGAAACCT AATTCCAAAT CTATGCAAGA CGGCTGGGGG 
GAGAGTGACG GTCCAGTGAC GGGAACTCGC CATCCCAGCT GGGAGGAGGA GGATGATGGA 
GGAGTCTGGA ACACCGCCGG CTCTCAGGGA AGTGCTTCCT CCCACAACTC AGCAAGCTGG 
GGACAAGGAG GAAAGAAGCA AATGAAGTGC TCACTGAAAG GAGGAAACAA TGATTCATGG 
ATGAATCCTC TTGCCAAACA GTTTTCAAAT ATGGGATTGC TGAGTCAAAC TGAAGATAAT 
CCAGGCAGCA AAATGGATTT GTCTGTAGGG AGCCTTCCAG ATAAAAAATT TGACGTGGAC 
AAGCGAGCAA TGAATCTCGG GGATTTTAAT GATATCATGA GGAAGGATCG ATCTGGGTTC 
CGTCCACCTA ATTCCAAAGA CATGGGAACC ACAGATAGTG GGCCTTATTT CGAGAAGCTG 
ACTTTGCCTT TCTCCAATCA AGATGGGTGC CTTGGGGATG AGGCTCCCTG CTCTCCCTTC 
TCCCCTTCTC CCAGCTACAA GCTGTCTCCC TCTGGTTCCA CACTACCCAA CGTCAGCCTT 
GGAGCAATCG GCACAGGGCT CAACCCCCAA AACTTCGCTG CTAGACAAGG TGGCAATCAT 
GGTTTGTTTG GAAATAGCAC AGCACAATCG AGAGGTATGC ACACACCAGT GCAGCCACTA 
AATTCTTCTC CTAATCTCCG GGCGCAAGTG CCTCCCCAGT TTATTTCCCC CCAGGTTTCT 
GCCTCCATGC TCAAGCAGTT TCCCAACAGT GGCCTGAATC CAGGTCTTTT CAATGTGGGG 
CCCCAGTTAT CTCCTCAACA GATTGCCATG CTGAGCCAGC TTCCACAAAT TCCCCAGTTT 
CAGTTGGCAT GTCAGCTTCT CCTGCAGCAG CAGCAACAGC AGCAGTTGCT ACAGAACCAG 
AGGAAGCTTT CTCAAGCTGT GCGCCAGCAG CAAGAGCAGC AGCTGGCTCG AATGGTGAGC 
GCTCTCCAGC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AGAGGCAGCC TGGCATGAAG 
CACTCACCCT CTCATCCTGT TGGGCCCAAG CCACATCTGG ACAACATGGT ACCCAACGCT 
CTGAATGTGG GGCTCCCAGA CCTTCAAACC AAAGGGCCAA TACCTGGATA TGGTTCTGGC 
TTCAGCTCTG GCGGCATGGA CTATGGCATG GTTGGTGGGA AGGAGGCTGG AACAGAGTCT 
CGCTTTAAAC AGTGGACCTC CATGATGGAG GGACTGCCCT CTGTAGCCAC ACAGGAAGCC 
AATATGCACA AAAATGGCGC TATTGTGGCC CCTGGTAAGA CTCGAGGAGG GTCACCGTAC 
AACCAGTTTG ATATCATCCC GAGTGACACA CTGGGTGGCC ATACGGGTCC TGCTGGTGAT 
AGCTGGTTAC CTGCCAAATC TCCACCAACA AATAAAATCG GAAGTAAATC CAGCAATGCC 
AGTTGGCCTC CAGAATTCCA ACCAGGAGTG CCATGGAAAG GTATCCAAAA CATTGACCCT 
GAATCTGACC CCTATGTCAC CCCAGGAAGT GTACTGGGGG GTACAGCCAC ATCTCCCATT 
GTAGATACTG ACCACCAACT TCTGCGGGAT AACACCACAG GGTCTAATTC TTCCCTCAAC 
ACCTCGCTGC CTTCACCTGG TGCCTGGCCC TACAGTGCCT CTGACAACTC CTTTACCAAC 
GTTCATAGCA CTTCAGCAAA GTTCCCTGAT TACAAATCAA CATGGTCCCC AGATCCCATA 
GGACACAACC CCACTCATCT CTCCAACAAG ATGTGGAAAA ACCATATTTC CTCAAGGAAC 
ACTACACCGC TGCCCCGCCC ACCTCCCGGT CTGACCAACC CCAAACCATC ATCTCCCTGG 
AGCAGCACAG CACCCCGATC AGTCAGGGGG TGGGGGACAC AGGACTCACG GCTTGCCTCG 
GCCTCTACCT GGAGTGATGG TGGTTCAGTT CGTCCCAGTT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATTGATGG GTCAACGTTG AGAACCATCT GCATGCAGCA TGGCCCACTG 
CTGACATTCC ATCTGAACCT AACCCAGGGC ACTGCCCTGA TCCGATACAG CACCAAACAG 
GAGGCGGCCA AGGCCCAAAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTG 
GCTGAGTTTG CCACCGATGA TGAAGTTAGC CGCTTTCTGG CACAAGCTCA GCCCCCTACA 
CCTGCAGCAA CCCCAAGTGC ACCCGCCGCA GGTTGGCAGT CGCTGGAGAC CGGCCAGACC 
CAGTCAGATC CCGTCGGACC TGCTTTGAAT CTTTTTGGTG GGTCCACAGG GCTCGGGCAA 
TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG GCCGATCTTG CTGGCGCTTC GTTGTGGGGG 
CCCCCAAACT ATTCTTCTAG CTTGTGGGGA GTCCCAGCTG TGGAAGATCC CCATAGGATG 
GGCAGCCCTG CTCCCTTACT ACCTGGTGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_014117342.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972817.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

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CloneID	OCe38896
Clone ID Related Accession (Same CDS sequence)	XM_014117342.1
Accession Version	XM_014117342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGA AGGAGCAAGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA ACGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCGCCGT CCTGCATGCT CCTTGGGGGC 
GGAGCAGGGC CTCCTCCCTC CACAGCACCT GGAGCAAACT CAAACAACGC ACAAGTGACA 
GGAACGCTGC TGCAGAGTGA CAGTGGGACT GCACCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAA CGGCGCCTCC 
CCCAACCCAA TTCACACCTG GGACAAGGTG ATTGTAGACG GGTCTGACGT GGAAGAGTGG 
CCTTGTATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAAGTTCT GGGAACGAAT GTAATCTTGG GGTCTGGAAA 
TCTGACCCTA AGACTAAATC TGTTCAATCT TCCAACTCTA CAACAGAGAG CAACAATGGA 
CTAGGAAATT GGAGGAATGT GAGTGGTCAG GATAGAATTG GACCTGGCTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAAGGGG CATTGGAAAC GGATACTAGT AATTCCAGTG CACAGGTTAG CACAGTAGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAAC AGGCTCAGAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTTAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG AATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAAGATAGAG AGGACTCCTG GAAAGGAGCT 
TCTGTTCAGA AATCATCTGG GTCAAAAAAT GACTCTTGGG ACAATAACAG GTCTACGGGT 
GGGTCCTGGA ACTTTGGCCC TCAGGACTCT AATGACAACA AATGGGGTGA AGGGAACAAA 
ATGACATCTG GGGTCTCTCA GGGAGAATGG AAACAGCCGA CTGGGTCTGA TGAGTTGAAA 
ATTGGAGAAT GGAGTGGTCC AAACCAACCA AATTCTAGCA CTGGAGCATG GGACAATCAA 
AAGGGCCACC CCCTCCCTGA AAACCAAGGC AATGCCCAGG CTCCCTGTTG GGGAAGATCT 
TCCAGCTCCA CAGGAAGTGA AGTTGGAGGT CAAAGCACTG GAAGCAACCA CAAAGCAGGA 
AGTAGTGACA GTCATAATTC TGGCCGTCGG TCATACCGGC CCACACATCC TGATTGTCAG 
GCTGTCTTAC AGACTCTTTT GAGCCGAACT GATTTGGACC CCAGGGTGCT CTCAAACACT 
GGCTGGGGCC AAACTCAAAT TAAGCAGGAC ACTGTGTGGG ATATTGAAGA GGTGCCAAGG 
CCTGAGGGGA AATCGGACAA AGGAACTGAG GGGTGGGAGA GCGCTGCCAC ACAGACCAAG 
AACTCAGGGG GCTGGGGAGA TGCACCCAGC CAAAGCAATC AAATGAAGTC TGGATGGGGG 
GAGCTCTCAG CCTCTACAGA GTGGAAAGAC CCCAAGAACA CAGGAGGCTG GAATGACTAT 
AAGAACAACA ACTCTTCCAA CTGGGGAGGC GGACGACCAG ATGAAAAGAC ATCTTCCTCT 
TGGAATGAGA ACTCCAGCAA GGATCAGGGG TGGGGAGGGG GACGCCAGCC CAATCAAGGA 
TGGACTTCTG GGAAGAATGG TTGGGGAGAG GAAGTTGATC AGGCGAAAAA CAGCAGTTGG 
GAAAGTTCTG CAAGCAAACC TGTGTCTGGA TGGGGAGAAG GAGGGCAGAA TGAAATTGGA 
ACTTGGGGGA ACAGCGGGAA TGCAAGCCTA GCTTCCAAAG GTGGGTGGGA AGATTGCAAA 
AGGTCTCCAG CGTGGACTGA GACTGGCAGA CAGCCCAATT CCTGGCATAA ACAGCACCAA 
CAGCAGCAGC AGCCACAGCA GCCACCGCCA CCACAACCAG AGGCTTCTGG TTCATGGGGA 
GGCCCCCCAC CACCACCTCC AGGCAACGGA CGACCTTCCA ATTCCAACTG GAGCAGTGGG 
CCACAGCCAG TAACCCCTAA GGACGAGGAA CCCAGTGGTT GGGAAGAGCC ATCCCCACAG 
TCAATTAGTC GGAAAATGGA CATCGATGAT GGCACTTCAG CATGGGGAGA CCCTAACAGC 
TATAACTACA AGAATGTGAA TCTGTGGGAT AAGAATTCCC AAGGGGGCCC AGCACCTCGA 
GAACCAAACC TGCCTACCCC GATGACCAGT AAATCAGCAT CAGTCTGGAG CAAAAGCACA 
CCACCTGCTC CAGATAATGG TACTTCAGCT TGGGGTGAGC CAAACGAAAG CAGTCCTGGG 
TGGGGCGAAA TGGATGACAC GGGAGCATCG ACCACGGGCT GGGGGAATGC ACCCTCCAAC 
GCTCCAAATG CCATGAAACC TAATTCCAAA TCTATGCAAG ACGGCTGGGG GGAGAGTGAC 
GGTCCAGTGA CGGGAACTCG CCATCCCAGC TGGGAGGAGG AGGATGATGG AGGAGTCTGG 
AACACCGCCG GCTCTCAGGG AAGTGCTTCC TCCCACAACT CAGCAAGCTG GGGACAAGGA 
GGAAAGAAGC AAATGAAGTG CTCACTGAAA GGAGGAAACA ATGATTCATG GATGAATCCT 
CTTGCCAAAC AGTTTTCAAA TATGGGATTG CTGAGTCAAA CTGAAGATAA TCCAGGCAGC 
AAAATGGATT TGTCTGTAGG GAGCCTTCCA GATAAAAAAT TTGACGTGGA CAAGCGAGCA 
ATGAATCTCG GGGATTTTAA TGATATCATG AGGAAGGATC GATCTGGGTT CCGTCCACCT 
AATTCCAAAG ACATGGGAAC CACAGATAGT GGGCCTTATT TCGAGAAGCT GACTTTGCCT 
TTCTCCAATC AAGATGGGTG CCTTGGGGAT GAGGCTCCCT GCTCTCCCTT CTCCCCTTCT 
CCCAGCTACA AGCTGTCTCC CTCTGGTTCC ACACTACCCA ACGTCAGCCT TGGAGCAATC 
GGCACAGGGC TCAACCCCCA AAACTTCGCT GCTAGACAAG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGTATG CACACACCAG TGCAGCCACT AAATTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTTATTTCCC CCCAGGTTTC TGCCTCCATG 
CTCAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAATGTGGG GCCCCAGTTA 
TCTCCTCAAC AGATTGCCAT GCTGAGCCAG CTTCCACAAA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGC TACAGAACCA GAGGAAGCTT 
TCTCAAGCTG TGCGCCAGCA GCAAGAGCAG CAGCTGGCTC GAATGGTGAG CGCTCTCCAG 
CAACAGCAGC AACAGCAGCA GCAGCAGCAG CAGAGGCAGC CTGGCATGAA GCACTCACCC 
TCTCATCCTG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCT 
GGCGGCATGG ACTATGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGGACTGCCC TCTGTAGCCA CACAGGAAGC CAATATGCAC 
AAAAATGGCG CTATTGTGGC CCCTGGTAAG ACTCGAGGAG GGTCACCGTA CAACCAGTTT 
GATATCATCC CGAGTGACAC ACTGGGTGGC CATACGGGTC CTGCTGGTGA TAGCTGGTTA 
CCTGCCAAAT CTCCACCAAC AAATAAAATC GGAAGTAAAT CCAGCAATGC CAGTTGGCCT 
CCAGAATTCC AACCAGGAGT GCCATGGAAA GGTATCCAAA ACATTGACCC TGAATCTGAC 
CCCTATGTCA CCCCAGGAAG TGTACTGGGG GGTACAGCCA CATCTCCCAT TGTAGATACT 
GACCACCAAC TTCTGCGGGA TAACACCACA GGGTCTAATT CTTCCCTCAA CACCTCGCTG 
CCTTCACCTG GTGCCTGGCC CTACAGTGCC TCTGACAACT CCTTTACCAA CGTTCATAGC 
ACTTCAGCAA AGTTCCCTGA TTACAAATCA ACATGGTCCC CAGATCCCAT AGGACACAAC 
CCCACTCATC TCTCCAACAA GATGTGGAAA AACCATATTT CCTCAAGGAA CACTACACCG 
CTGCCCCGCC CACCTCCCGG TCTGACCAAC CCCAAACCAT CATCTCCCTG GAGCAGCACA 
GCACCCCGAT CAGTCAGGGG GTGGGGGACA CAGGACTCAC GGCTTGCCTC GGCCTCTACC 
TGGAGTGATG GTGGTTCAGT TCGTCCCAGT TACTGGCTGG TTCTTCACAA TCTCACTCCA 
CAGATTGATG GGTCAACGTT GAGAACCATC TGCATGCAGC ATGGCCCACT GCTGACATTC 
CATCTGAACC TAACCCAGGG CACTGCCCTG ATCCGATACA GCACCAAACA GGAGGCGGCC 
AAGGCCCAAA CTGCACTGCA CATGTGTGTG TTGGGAAACA CTACCATCCT GGCTGAGTTT 
GCCACCGATG ATGAAGTTAG CCGCTTTCTG GCACAAGCTC AGCCCCCTAC ACCTGCAGCA 
ACCCCAAGTG CACCCGCCGC AGGTTGGCAG TCGCTGGAGA CCGGCCAGAC CCAGTCAGAT 
CCCGTCGGAC CTGCTTTGAA TCTTTTTGGT GGGTCCACAG GGCTCGGGCA ATGGAGCAGC 
AGTGCTGGTG GCAGCAGCGG GGCCGATCTT GCTGGCGCTT CGTTGTGGGG GCCCCCAAAC 
TATTCTTCTA GCTTGTGGGG AGTCCCAGCT GTGGAAGATC CCCATAGGAT GGGCAGCCCT 
GCTCCCTTAC TACCTGGTGA CCTTCTGGGA GGAGGGTCGG ATTCAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972817.1
CDS	390..5900
Translation	MREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_014117342.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117342.1

ORF Insert Sequence:

ATGAGAGAGA AGGAGCAAGA AAGGGAAGAA CAGTTAATGG AAGACAAGAA AAGGAAGAAA 
GAGGATAAAA AGAAAAAAGA AGCCACTCAG AAGGTCACGG AACAAAAAAC CAAAGTGCCC 
GAAGTGACGA AACCAAGTTT AAGCCAACCA ACGGCCGCCA GCCCAATTGG CAGCTCTCCA 
TCGCCACCAG TCAATGGTGG CAACAATGCC AAAAGGGTGG CAGTGCCGAA CGGACAACCG 
CCAAGCGCCG CCCGCTACAT GCCTCGGGAG GTGCCGCCGC GATTCCGTTG CCAGCAGGAC 
CACAAAGTGT TACTAAAACG TGGGCAGCCC CCTCCGCCGT CCTGCATGCT CCTTGGGGGC 
GGAGCAGGGC CTCCTCCCTC CACAGCACCT GGAGCAAACT CAAACAACGC ACAAGTGACA 
GGAACGCTGC TGCAGAGTGA CAGTGGGACT GCACCAGAGT CAACCCTTGG AGGTGCTGCT 
GCTTCAAATT ATGCAAATTC CACTTGGGGC CCGGGAGCCT CCTCCAACAA CGGCGCCTCC 
CCCAACCCAA TTCACACCTG GGACAAGGTG ATTGTAGACG GGTCTGACGT GGAAGAGTGG 
CCTTGTATTG CCAGCAAAGA CACTGAGTCT TCTTCCGAAA ACACCACCGA TAACAACAGT 
GCCTCGAACC CTGGCTCTGA GAAGAGCACT CTGCCAGGAA GCACCACTAG TAACAAAGGA 
AAAGGGAGCC AGTGCCAGTC TGCAAGTTCT GGGAACGAAT GTAATCTTGG GGTCTGGAAA 
TCTGACCCTA AGACTAAATC TGTTCAATCT TCCAACTCTA CAACAGAGAG CAACAATGGA 
CTAGGAAATT GGAGGAATGT GAGTGGTCAG GATAGAATTG GACCTGGCTC TGGCTTCAGC 
AACTTTAACC CAAATAGCAA CCCATCTGCC TGGCCAGCGC TGGTCCAAGA AGGAAATTCT 
AGGAAAGGGG CATTGGAAAC GGATACTAGT AATTCCAGTG CACAGGTTAG CACAGTAGGT 
CAGGCATCCA GGGAACAGCA GTCAAAGATG GAAAATGCGG GTGTTAATTT TGTTGTCTCT 
GGCAGAGAAC AGGCTCAGAT TCATAACACT GATGGACCAA AAAATGGAAA CACTAACTCC 
TTGAACTTAA GTTCACCAAA CCCCATGGAG AATAAGGGAA TGCCCTTTGG AATGGGCTTG 
GGGAACGCCT CCAGGAGCAC TGATGCCCCT TCACAAAGCA CTGGAGATCG AAAGACTGGG 
AGTGTTGGAT CTTGGGGTGC AGCTAGGGGG CCTTCTGGAA CTGACACAGC CTCTGGACAA 
AGCAATTCTG GAAACAATGG GAACAATGGA AAAGATAGAG AGGACTCCTG GAAAGGAGCT 
TCTGTTCAGA AATCATCTGG GTCAAAAAAT GACTCTTGGG ACAATAACAG GTCTACGGGT 
GGGTCCTGGA ACTTTGGCCC TCAGGACTCT AATGACAACA AATGGGGTGA AGGGAACAAA 
ATGACATCTG GGGTCTCTCA GGGAGAATGG AAACAGCCGA CTGGGTCTGA TGAGTTGAAA 
ATTGGAGAAT GGAGTGGTCC AAACCAACCA AATTCTAGCA CTGGAGCATG GGACAATCAA 
AAGGGCCACC CCCTCCCTGA AAACCAAGGC AATGCCCAGG CTCCCTGTTG GGGAAGATCT 
TCCAGCTCCA CAGGAAGTGA AGTTGGAGGT CAAAGCACTG GAAGCAACCA CAAAGCAGGA 
AGTAGTGACA GTCATAATTC TGGCCGTCGG TCATACCGGC CCACACATCC TGATTGTCAG 
GCTGTCTTAC AGACTCTTTT GAGCCGAACT GATTTGGACC CCAGGGTGCT CTCAAACACT 
GGCTGGGGCC AAACTCAAAT TAAGCAGGAC ACTGTGTGGG ATATTGAAGA GGTGCCAAGG 
CCTGAGGGGA AATCGGACAA AGGAACTGAG GGGTGGGAGA GCGCTGCCAC ACAGACCAAG 
AACTCAGGGG GCTGGGGAGA TGCACCCAGC CAAAGCAATC AAATGAAGTC TGGATGGGGG 
GAGCTCTCAG CCTCTACAGA GTGGAAAGAC CCCAAGAACA CAGGAGGCTG GAATGACTAT 
AAGAACAACA ACTCTTCCAA CTGGGGAGGC GGACGACCAG ATGAAAAGAC ATCTTCCTCT 
TGGAATGAGA ACTCCAGCAA GGATCAGGGG TGGGGAGGGG GACGCCAGCC CAATCAAGGA 
TGGACTTCTG GGAAGAATGG TTGGGGAGAG GAAGTTGATC AGGCGAAAAA CAGCAGTTGG 
GAAAGTTCTG CAAGCAAACC TGTGTCTGGA TGGGGAGAAG GAGGGCAGAA TGAAATTGGA 
ACTTGGGGGA ACAGCGGGAA TGCAAGCCTA GCTTCCAAAG GTGGGTGGGA AGATTGCAAA 
AGGTCTCCAG CGTGGACTGA GACTGGCAGA CAGCCCAATT CCTGGCATAA ACAGCACCAA 
CAGCAGCAGC AGCCACAGCA GCCACCGCCA CCACAACCAG AGGCTTCTGG TTCATGGGGA 
GGCCCCCCAC CACCACCTCC AGGCAACGGA CGACCTTCCA ATTCCAACTG GAGCAGTGGG 
CCACAGCCAG TAACCCCTAA GGACGAGGAA CCCAGTGGTT GGGAAGAGCC ATCCCCACAG 
TCAATTAGTC GGAAAATGGA CATCGATGAT GGCACTTCAG CATGGGGAGA CCCTAACAGC 
TATAACTACA AGAATGTGAA TCTGTGGGAT AAGAATTCCC AAGGGGGCCC AGCACCTCGA 
GAACCAAACC TGCCTACCCC GATGACCAGT AAATCAGCAT CAGTCTGGAG CAAAAGCACA 
CCACCTGCTC CAGATAATGG TACTTCAGCT TGGGGTGAGC CAAACGAAAG CAGTCCTGGG 
TGGGGCGAAA TGGATGACAC GGGAGCATCG ACCACGGGCT GGGGGAATGC ACCCTCCAAC 
GCTCCAAATG CCATGAAACC TAATTCCAAA TCTATGCAAG ACGGCTGGGG GGAGAGTGAC 
GGTCCAGTGA CGGGAACTCG CCATCCCAGC TGGGAGGAGG AGGATGATGG AGGAGTCTGG 
AACACCGCCG GCTCTCAGGG AAGTGCTTCC TCCCACAACT CAGCAAGCTG GGGACAAGGA 
GGAAAGAAGC AAATGAAGTG CTCACTGAAA GGAGGAAACA ATGATTCATG GATGAATCCT 
CTTGCCAAAC AGTTTTCAAA TATGGGATTG CTGAGTCAAA CTGAAGATAA TCCAGGCAGC 
AAAATGGATT TGTCTGTAGG GAGCCTTCCA GATAAAAAAT TTGACGTGGA CAAGCGAGCA 
ATGAATCTCG GGGATTTTAA TGATATCATG AGGAAGGATC GATCTGGGTT CCGTCCACCT 
AATTCCAAAG ACATGGGAAC CACAGATAGT GGGCCTTATT TCGAGAAGCT GACTTTGCCT 
TTCTCCAATC AAGATGGGTG CCTTGGGGAT GAGGCTCCCT GCTCTCCCTT CTCCCCTTCT 
CCCAGCTACA AGCTGTCTCC CTCTGGTTCC ACACTACCCA ACGTCAGCCT TGGAGCAATC 
GGCACAGGGC TCAACCCCCA AAACTTCGCT GCTAGACAAG GTGGCAATCA TGGTTTGTTT 
GGAAATAGCA CAGCACAATC GAGAGGTATG CACACACCAG TGCAGCCACT AAATTCTTCT 
CCTAATCTCC GGGCGCAAGT GCCTCCCCAG TTTATTTCCC CCCAGGTTTC TGCCTCCATG 
CTCAAGCAGT TTCCCAACAG TGGCCTGAAT CCAGGTCTTT TCAATGTGGG GCCCCAGTTA 
TCTCCTCAAC AGATTGCCAT GCTGAGCCAG CTTCCACAAA TTCCCCAGTT TCAGTTGGCA 
TGTCAGCTTC TCCTGCAGCA GCAGCAACAG CAGCAGTTGC TACAGAACCA GAGGAAGCTT 
TCTCAAGCTG TGCGCCAGCA GCAAGAGCAG CAGCTGGCTC GAATGGTGAG CGCTCTCCAG 
CAACAGCAGC AACAGCAGCA GCAGCAGCAG CAGAGGCAGC CTGGCATGAA GCACTCACCC 
TCTCATCCTG TTGGGCCCAA GCCACATCTG GACAACATGG TACCCAACGC TCTGAATGTG 
GGGCTCCCAG ACCTTCAAAC CAAAGGGCCA ATACCTGGAT ATGGTTCTGG CTTCAGCTCT 
GGCGGCATGG ACTATGGCAT GGTTGGTGGG AAGGAGGCTG GAACAGAGTC TCGCTTTAAA 
CAGTGGACCT CCATGATGGA GGGACTGCCC TCTGTAGCCA CACAGGAAGC CAATATGCAC 
AAAAATGGCG CTATTGTGGC CCCTGGTAAG ACTCGAGGAG GGTCACCGTA CAACCAGTTT 
GATATCATCC CGAGTGACAC ACTGGGTGGC CATACGGGTC CTGCTGGTGA TAGCTGGTTA 
CCTGCCAAAT CTCCACCAAC AAATAAAATC GGAAGTAAAT CCAGCAATGC CAGTTGGCCT 
CCAGAATTCC AACCAGGAGT GCCATGGAAA GGTATCCAAA ACATTGACCC TGAATCTGAC 
CCCTATGTCA CCCCAGGAAG TGTACTGGGG GGTACAGCCA CATCTCCCAT TGTAGATACT 
GACCACCAAC TTCTGCGGGA TAACACCACA GGGTCTAATT CTTCCCTCAA CACCTCGCTG 
CCTTCACCTG GTGCCTGGCC CTACAGTGCC TCTGACAACT CCTTTACCAA CGTTCATAGC 
ACTTCAGCAA AGTTCCCTGA TTACAAATCA ACATGGTCCC CAGATCCCAT AGGACACAAC 
CCCACTCATC TCTCCAACAA GATGTGGAAA AACCATATTT CCTCAAGGAA CACTACACCG 
CTGCCCCGCC CACCTCCCGG TCTGACCAAC CCCAAACCAT CATCTCCCTG GAGCAGCACA 
GCACCCCGAT CAGTCAGGGG GTGGGGGACA CAGGACTCAC GGCTTGCCTC GGCCTCTACC 
TGGAGTGATG GTGGTTCAGT TCGTCCCAGT TACTGGCTGG TTCTTCACAA TCTCACTCCA 
CAGATTGATG GGTCAACGTT GAGAACCATC TGCATGCAGC ATGGCCCACT GCTGACATTC 
CATCTGAACC TAACCCAGGG CACTGCCCTG ATCCGATACA GCACCAAACA GGAGGCGGCC 
AAGGCCCAAA CTGCACTGCA CATGTGTGTG TTGGGAAACA CTACCATCCT GGCTGAGTTT 
GCCACCGATG ATGAAGTTAG CCGCTTTCTG GCACAAGCTC AGCCCCCTAC ACCTGCAGCA 
ACCCCAAGTG CACCCGCCGC AGGTTGGCAG TCGCTGGAGA CCGGCCAGAC CCAGTCAGAT 
CCCGTCGGAC CTGCTTTGAA TCTTTTTGGT GGGTCCACAG GGCTCGGGCA ATGGAGCAGC 
AGTGCTGGTG GCAGCAGCGG GGCCGATCTT GCTGGCGCTT CGTTGTGGGG GCCCCCAAAC 
TATTCTTCTA GCTTGTGGGG AGTCCCAGCT GTGGAAGATC CCCATAGGAT GGGCAGCCCT 
GCTCCCTTAC TACCTGGTGA CCTTCTGGGA GGAGGGTCGG ATTCAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_014117344.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972819.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

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CloneID	OCe38897
Clone ID Related Accession (Same CDS sequence)	XM_014117344.1
Accession Version	XM_014117344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GATTCCAAAT CTATGCAAGA CGGCTGGGGG 
GAGAGTGACG GTCCAGTGAC GGGAACTCGC CATCCCAGCT GGGAGGAGGA GGATGATGGA 
GGAGTCTGGA ACACCGCCGG CTCTCAGGGA AGTGCTTCCT CCCACAACTC AGCAAGCTGG 
GGACAAGGAG GAAAGAAGCA AATGAAGTGC TCACTGAAAG GAGGAAACAA TGATTCATGG 
ATGAATCCTC TTGCCAAACA GTTTTCAAAT ATGGGATTGC TGAGTCAAAC TGAAGATAAT 
CCAGGCAGCA AAATGGATTT GTCTGTAGGG AGCCTTCCAG ATAAAAAATT TGACGTGGAC 
AAGCGAGCAA TGAATCTCGG GGATTTTAAT GATATCATGA GGAAGGATCG ATCTGGGTTC 
CGTCCACCTA ATTCCAAAGA CATGGGAACC ACAGATAGTG GGCCTTATTT CGAGAAGCTG 
ACTTTGCCTT TCTCCAATCA AGATGGGTGC CTTGGGGATG AGGCTCCCTG CTCTCCCTTC 
TCCCCTTCTC CCAGCTACAA GCTGTCTCCC TCTGGTTCCA CACTACCCAA CGTCAGCCTT 
GGAGCAATCG GCACAGGGCT CAACCCCCAA AACTTCGCTG CTAGACAAGG TGGCAATCAT 
GGTTTGTTTG GAAATAGCAC AGCACAATCG AGAGGTATGC ACACACCAGT GCAGCCACTA 
AATTCTTCTC CTAATCTCCG GGCGCAAGTG CCTCCCCAGT TTATTTCCCC CCAGGTTTCT 
GCCTCCATGC TCAAGCAGTT TCCCAACAGT GGCCTGAATC CAGGTCTTTT CAATGTGGGG 
CCCCAGTTAT CTCCTCAACA GATTGCCATG CTGAGCCAGC TTCCACAAAT TCCCCAGTTT 
CAGTTGGCAT GTCAGCTTCT CCTGCAGCAG CAGCAACAGC AGCAGTTGCT ACAGAACCAG 
AGGAAGCTTT CTCAAGCTGT GCGCCAGCAG CAAGAGCAGC AGCTGGCTCG AATGGTGAGC 
GCTCTCCAGC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AGAGGCAGCC TGGCATGAAG 
CACTCACCCT CTCATCCTGT TGGGCCCAAG CCACATCTGG ACAACATGGT ACCCAACGCT 
CTGAATGTGG GGCTCCCAGA CCTTCAAACC AAAGGGCCAA TACCTGGATA TGGTTCTGGC 
TTCAGCTCTG GCGGCATGGA CTATGGCATG GTTGGTGGGA AGGAGGCTGG AACAGAGTCT 
CGCTTTAAAC AGTGGACCTC CATGATGGAG GGACTGCCCT CTGTAGCCAC ACAGGAAGCC 
AATATGCACA AAAATGGCGC TATTGTGGCC CCTGGTAAGA CTCGAGGAGG GTCACCGTAC 
AACCAGTTTG ATATCATCCC GAGTGACACA CTGGGTGGCC ATACGGGTCC TGCTGGTGAT 
AGCTGGTTAC CTGCCAAATC TCCACCAACA AATAAAATCG GAAGTAAATC CAGCAATGCC 
AGTTGGCCTC CAGAATTCCA ACCAGGAGTG CCATGGAAAG GTATCCAAAA CATTGACCCT 
GAATCTGACC CCTATGTCAC CCCAGGAAGT GTACTGGGGG GTACAGCCAC ATCTCCCATT 
GTAGATACTG ACCACCAACT TCTGCGGGAT AACACCACAG GGTCTAATTC TTCCCTCAAC 
ACCTCGCTGC CTTCACCTGG TGCCTGGCCC TACAGTGCCT CTGACAACTC CTTTACCAAC 
GTTCATAGCA CTTCAGCAAA GTTCCCTGAT TACAAATCAA CATGGTCCCC AGATCCCATA 
GGACACAACC CCACTCATCT CTCCAACAAG ATGTGGAAAA ACCATATTTC CTCAAGGAAC 
ACTACACCGC TGCCCCGCCC ACCTCCCGGT CTGACCAACC CCAAACCATC ATCTCCCTGG 
AGCAGCACAG CACCCCGATC AGTCAGGGGG TGGGGGACAC AGGACTCACG GCTTGCCTCG 
GCCTCTACCT GGAGTGATGG TGGTTCAGTT CGTCCCAGTT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATTGATGG GTCAACGTTG AGAACCATCT GCATGCAGCA TGGCCCACTG 
CTGACATTCC ATCTGAACCT AACCCAGGGC ACTGCCCTGA TCCGATACAG CACCAAACAG 
GAGGCGGCCA AGGCCCAAAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTG 
GCTGAGTTTG CCACCGATGA TGAAGTTAGC CGCTTTCTGG CACAAGCTCA GCCCCCTACA 
CCTGCAGCAA CCCCAAGTGC ACCCGCCGCA GGTTGGCAGT CGCTGGAGAC CGGCCAGACC 
CAGTCAGATC CCGTCGGACC TGCTTTGAAT CTTTTTGGTG GGTCCACAGG GCTCGGGCAA 
TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG GCCGATCTTG CTGGCGCTTC GTTGTGGGGG 
CCCCCAAACT ATTCTTCTAG CTTGTGGGGA GTCCCAGCTG TGGAAGATCC CCATAGGATG 
GGCAGCCCTG CTCCCTTACT ACCTGGTGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972819.1
CDS	245..5704
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASDSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKLTLPFSNQDGCLGDEAPCSPFSPSPSYKLSPSGSTLPNVSLGAIGTGLNPQNFAARQGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_014117344.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117344.1

ORF Insert Sequence:

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GATTCCAAAT CTATGCAAGA CGGCTGGGGG 
GAGAGTGACG GTCCAGTGAC GGGAACTCGC CATCCCAGCT GGGAGGAGGA GGATGATGGA 
GGAGTCTGGA ACACCGCCGG CTCTCAGGGA AGTGCTTCCT CCCACAACTC AGCAAGCTGG 
GGACAAGGAG GAAAGAAGCA AATGAAGTGC TCACTGAAAG GAGGAAACAA TGATTCATGG 
ATGAATCCTC TTGCCAAACA GTTTTCAAAT ATGGGATTGC TGAGTCAAAC TGAAGATAAT 
CCAGGCAGCA AAATGGATTT GTCTGTAGGG AGCCTTCCAG ATAAAAAATT TGACGTGGAC 
AAGCGAGCAA TGAATCTCGG GGATTTTAAT GATATCATGA GGAAGGATCG ATCTGGGTTC 
CGTCCACCTA ATTCCAAAGA CATGGGAACC ACAGATAGTG GGCCTTATTT CGAGAAGCTG 
ACTTTGCCTT TCTCCAATCA AGATGGGTGC CTTGGGGATG AGGCTCCCTG CTCTCCCTTC 
TCCCCTTCTC CCAGCTACAA GCTGTCTCCC TCTGGTTCCA CACTACCCAA CGTCAGCCTT 
GGAGCAATCG GCACAGGGCT CAACCCCCAA AACTTCGCTG CTAGACAAGG TGGCAATCAT 
GGTTTGTTTG GAAATAGCAC AGCACAATCG AGAGGTATGC ACACACCAGT GCAGCCACTA 
AATTCTTCTC CTAATCTCCG GGCGCAAGTG CCTCCCCAGT TTATTTCCCC CCAGGTTTCT 
GCCTCCATGC TCAAGCAGTT TCCCAACAGT GGCCTGAATC CAGGTCTTTT CAATGTGGGG 
CCCCAGTTAT CTCCTCAACA GATTGCCATG CTGAGCCAGC TTCCACAAAT TCCCCAGTTT 
CAGTTGGCAT GTCAGCTTCT CCTGCAGCAG CAGCAACAGC AGCAGTTGCT ACAGAACCAG 
AGGAAGCTTT CTCAAGCTGT GCGCCAGCAG CAAGAGCAGC AGCTGGCTCG AATGGTGAGC 
GCTCTCCAGC AACAGCAGCA ACAGCAGCAG CAGCAGCAGC AGAGGCAGCC TGGCATGAAG 
CACTCACCCT CTCATCCTGT TGGGCCCAAG CCACATCTGG ACAACATGGT ACCCAACGCT 
CTGAATGTGG GGCTCCCAGA CCTTCAAACC AAAGGGCCAA TACCTGGATA TGGTTCTGGC 
TTCAGCTCTG GCGGCATGGA CTATGGCATG GTTGGTGGGA AGGAGGCTGG AACAGAGTCT 
CGCTTTAAAC AGTGGACCTC CATGATGGAG GGACTGCCCT CTGTAGCCAC ACAGGAAGCC 
AATATGCACA AAAATGGCGC TATTGTGGCC CCTGGTAAGA CTCGAGGAGG GTCACCGTAC 
AACCAGTTTG ATATCATCCC GAGTGACACA CTGGGTGGCC ATACGGGTCC TGCTGGTGAT 
AGCTGGTTAC CTGCCAAATC TCCACCAACA AATAAAATCG GAAGTAAATC CAGCAATGCC 
AGTTGGCCTC CAGAATTCCA ACCAGGAGTG CCATGGAAAG GTATCCAAAA CATTGACCCT 
GAATCTGACC CCTATGTCAC CCCAGGAAGT GTACTGGGGG GTACAGCCAC ATCTCCCATT 
GTAGATACTG ACCACCAACT TCTGCGGGAT AACACCACAG GGTCTAATTC TTCCCTCAAC 
ACCTCGCTGC CTTCACCTGG TGCCTGGCCC TACAGTGCCT CTGACAACTC CTTTACCAAC 
GTTCATAGCA CTTCAGCAAA GTTCCCTGAT TACAAATCAA CATGGTCCCC AGATCCCATA 
GGACACAACC CCACTCATCT CTCCAACAAG ATGTGGAAAA ACCATATTTC CTCAAGGAAC 
ACTACACCGC TGCCCCGCCC ACCTCCCGGT CTGACCAACC CCAAACCATC ATCTCCCTGG 
AGCAGCACAG CACCCCGATC AGTCAGGGGG TGGGGGACAC AGGACTCACG GCTTGCCTCG 
GCCTCTACCT GGAGTGATGG TGGTTCAGTT CGTCCCAGTT ACTGGCTGGT TCTTCACAAT 
CTCACTCCAC AGATTGATGG GTCAACGTTG AGAACCATCT GCATGCAGCA TGGCCCACTG 
CTGACATTCC ATCTGAACCT AACCCAGGGC ACTGCCCTGA TCCGATACAG CACCAAACAG 
GAGGCGGCCA AGGCCCAAAC TGCACTGCAC ATGTGTGTGT TGGGAAACAC TACCATCCTG 
GCTGAGTTTG CCACCGATGA TGAAGTTAGC CGCTTTCTGG CACAAGCTCA GCCCCCTACA 
CCTGCAGCAA CCCCAAGTGC ACCCGCCGCA GGTTGGCAGT CGCTGGAGAC CGGCCAGACC 
CAGTCAGATC CCGTCGGACC TGCTTTGAAT CTTTTTGGTG GGTCCACAGG GCTCGGGCAA 
TGGAGCAGCA GTGCTGGTGG CAGCAGCGGG GCCGATCTTG CTGGCGCTTC GTTGTGGGGG 
CCCCCAAACT ATTCTTCTAG CTTGTGGGGA GTCCCAGCTG TGGAAGATCC CCATAGGATG 
GGCAGCCCTG CTCCCTTACT ACCTGGTGAC CTTCTGGGAG GAGGGTCGGA TTCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_022424397.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280105.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X13, mRNA.

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CloneID	OCe38898
Clone ID Related Accession (Same CDS sequence)	XM_022424397.1 , XM_014117345.1
Accession Version	XM_022424397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GTCTGGAGCA AAAGCACACC ACCTGCTCCA 
GATAATGGTA CTTCAGCTTG GGGTGAGCCA AACGAAAGCA GTCCTGGGTG GGGCGAAATG 
GATGACACGG GAGCATCGAC CACGGGCTGG GGGAATGCAC CCTCCAACGC TCCAAATGCC 
ATGAAACCTA ATTCCAAATC TATGCAAGAC GGCTGGGGGG AGAGTGACGG TCCAGTGACG 
GGAACTCGCC ATCCCAGCTG GGAGGAGGAG GATGATGGAG GAGTCTGGAA CACCGCCGGC 
TCTCAGGGAA GTGCTTCCTC CCACAACTCA GCAAGCTGGG GACAAGGAGG AAAGAAGCAA 
ATGAAGTGCT CACTGAAAGG AGGAAACAAT GATTCATGGA TGAATCCTCT TGCCAAACAG 
TTTTCAAATA TGGGATTGCT GAGTCAAACT GAAGATAATC CAGGCAGCAA AATGGATTTG 
TCTGTAGGGA GCCTTCCAGA TAAAAAATTT GACGTGGACA AGCGAGCAAT GAATCTCGGG 
GATTTTAATG ATATCATGAG GAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTC GAGAAGGGTG GCAATCATGG TTTGTTTGGA 
AATAGCACAG CACAATCGAG AGGTATGCAC ACACCAGTGC AGCCACTAAA TTCTTCTCCT 
AATCTCCGGG CGCAAGTGCC TCCCCAGTTT ATTTCCCCCC AGGTTTCTGC CTCCATGCTC 
AAGCAGTTTC CCAACAGTGG CCTGAATCCA GGTCTTTTCA ATGTGGGGCC CCAGTTATCT 
CCTCAACAGA TTGCCATGCT GAGCCAGCTT CCACAAATTC CCCAGTTTCA GTTGGCATGT 
CAGCTTCTCC TGCAGCAGCA GCAACAGCAG CAGTTGCTAC AGAACCAGAG GAAGCTTTCT 
CAAGCTGTGC GCCAGCAGCA AGAGCAGCAG CTGGCTCGAA TGGTGAGCGC TCTCCAGCAA 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG AGGCAGCCTG GCATGAAGCA CTCACCCTCT 
CATCCTGTTG GGCCCAAGCC ACATCTGGAC AACATGGTAC CCAACGCTCT GAATGTGGGG 
CTCCCAGACC TTCAAACCAA AGGGCCAATA CCTGGATATG GTTCTGGCTT CAGCTCTGGC 
GGCATGGACT ATGGCATGGT TGGTGGGAAG GAGGCTGGAA CAGAGTCTCG CTTTAAACAG 
TGGACCTCCA TGATGGAGGG ACTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACT CGAGGAGGGT CACCGTACAA CCAGTTTGAT 
ATCATCCCGA GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCCA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT ACTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TCCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACACCGCTG 
CCCCGCCCAC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGATCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TTGCCTCGGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCCAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACGTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACCGATGATG AAGTTAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCAGCAACC 
CCAAGTGCAC CCGCCGCAGG TTGGCAGTCG CTGGAGACCG GCCAGACCCA GTCAGATCCC 
GTCGGACCTG CTTTGAATCT TTTTGGTGGG TCCACAGGGC TCGGGCAATG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCT GGCGCTTCGT TGTGGGGGCC CCCAAACTAT 
TCTTCTAGCT TGTGGGGAGT CCCAGCTGTG GAAGATCCCC ATAGGATGGG CAGCCCTGCT 
CCCTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280105.1
CDS	344..5791
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_022424397.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424397.1

ORF Insert Sequence:

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GTCTGGAGCA AAAGCACACC ACCTGCTCCA 
GATAATGGTA CTTCAGCTTG GGGTGAGCCA AACGAAAGCA GTCCTGGGTG GGGCGAAATG 
GATGACACGG GAGCATCGAC CACGGGCTGG GGGAATGCAC CCTCCAACGC TCCAAATGCC 
ATGAAACCTA ATTCCAAATC TATGCAAGAC GGCTGGGGGG AGAGTGACGG TCCAGTGACG 
GGAACTCGCC ATCCCAGCTG GGAGGAGGAG GATGATGGAG GAGTCTGGAA CACCGCCGGC 
TCTCAGGGAA GTGCTTCCTC CCACAACTCA GCAAGCTGGG GACAAGGAGG AAAGAAGCAA 
ATGAAGTGCT CACTGAAAGG AGGAAACAAT GATTCATGGA TGAATCCTCT TGCCAAACAG 
TTTTCAAATA TGGGATTGCT GAGTCAAACT GAAGATAATC CAGGCAGCAA AATGGATTTG 
TCTGTAGGGA GCCTTCCAGA TAAAAAATTT GACGTGGACA AGCGAGCAAT GAATCTCGGG 
GATTTTAATG ATATCATGAG GAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTC GAGAAGGGTG GCAATCATGG TTTGTTTGGA 
AATAGCACAG CACAATCGAG AGGTATGCAC ACACCAGTGC AGCCACTAAA TTCTTCTCCT 
AATCTCCGGG CGCAAGTGCC TCCCCAGTTT ATTTCCCCCC AGGTTTCTGC CTCCATGCTC 
AAGCAGTTTC CCAACAGTGG CCTGAATCCA GGTCTTTTCA ATGTGGGGCC CCAGTTATCT 
CCTCAACAGA TTGCCATGCT GAGCCAGCTT CCACAAATTC CCCAGTTTCA GTTGGCATGT 
CAGCTTCTCC TGCAGCAGCA GCAACAGCAG CAGTTGCTAC AGAACCAGAG GAAGCTTTCT 
CAAGCTGTGC GCCAGCAGCA AGAGCAGCAG CTGGCTCGAA TGGTGAGCGC TCTCCAGCAA 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG AGGCAGCCTG GCATGAAGCA CTCACCCTCT 
CATCCTGTTG GGCCCAAGCC ACATCTGGAC AACATGGTAC CCAACGCTCT GAATGTGGGG 
CTCCCAGACC TTCAAACCAA AGGGCCAATA CCTGGATATG GTTCTGGCTT CAGCTCTGGC 
GGCATGGACT ATGGCATGGT TGGTGGGAAG GAGGCTGGAA CAGAGTCTCG CTTTAAACAG 
TGGACCTCCA TGATGGAGGG ACTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACT CGAGGAGGGT CACCGTACAA CCAGTTTGAT 
ATCATCCCGA GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCCA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT ACTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TCCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACACCGCTG 
CCCCGCCCAC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGATCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TTGCCTCGGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCCAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACGTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACCGATGATG AAGTTAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCAGCAACC 
CCAAGTGCAC CCGCCGCAGG TTGGCAGTCG CTGGAGACCG GCCAGACCCA GTCAGATCCC 
GTCGGACCTG CTTTGAATCT TTTTGGTGGG TCCACAGGGC TCGGGCAATG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCT GGCGCTTCGT TGTGGGGGCC CCCAAACTAT 
TCTTCTAGCT TGTGGGGAGT CCCAGCTGTG GAAGATCCCC ATAGGATGGG CAGCCCTGCT 
CCCTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_014117345.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972820.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

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CloneID	OCe38898
Clone ID Related Accession (Same CDS sequence)	XM_022424397.1 , XM_014117345.1
Accession Version	XM_014117345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GTCTGGAGCA AAAGCACACC ACCTGCTCCA 
GATAATGGTA CTTCAGCTTG GGGTGAGCCA AACGAAAGCA GTCCTGGGTG GGGCGAAATG 
GATGACACGG GAGCATCGAC CACGGGCTGG GGGAATGCAC CCTCCAACGC TCCAAATGCC 
ATGAAACCTA ATTCCAAATC TATGCAAGAC GGCTGGGGGG AGAGTGACGG TCCAGTGACG 
GGAACTCGCC ATCCCAGCTG GGAGGAGGAG GATGATGGAG GAGTCTGGAA CACCGCCGGC 
TCTCAGGGAA GTGCTTCCTC CCACAACTCA GCAAGCTGGG GACAAGGAGG AAAGAAGCAA 
ATGAAGTGCT CACTGAAAGG AGGAAACAAT GATTCATGGA TGAATCCTCT TGCCAAACAG 
TTTTCAAATA TGGGATTGCT GAGTCAAACT GAAGATAATC CAGGCAGCAA AATGGATTTG 
TCTGTAGGGA GCCTTCCAGA TAAAAAATTT GACGTGGACA AGCGAGCAAT GAATCTCGGG 
GATTTTAATG ATATCATGAG GAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTC GAGAAGGGTG GCAATCATGG TTTGTTTGGA 
AATAGCACAG CACAATCGAG AGGTATGCAC ACACCAGTGC AGCCACTAAA TTCTTCTCCT 
AATCTCCGGG CGCAAGTGCC TCCCCAGTTT ATTTCCCCCC AGGTTTCTGC CTCCATGCTC 
AAGCAGTTTC CCAACAGTGG CCTGAATCCA GGTCTTTTCA ATGTGGGGCC CCAGTTATCT 
CCTCAACAGA TTGCCATGCT GAGCCAGCTT CCACAAATTC CCCAGTTTCA GTTGGCATGT 
CAGCTTCTCC TGCAGCAGCA GCAACAGCAG CAGTTGCTAC AGAACCAGAG GAAGCTTTCT 
CAAGCTGTGC GCCAGCAGCA AGAGCAGCAG CTGGCTCGAA TGGTGAGCGC TCTCCAGCAA 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG AGGCAGCCTG GCATGAAGCA CTCACCCTCT 
CATCCTGTTG GGCCCAAGCC ACATCTGGAC AACATGGTAC CCAACGCTCT GAATGTGGGG 
CTCCCAGACC TTCAAACCAA AGGGCCAATA CCTGGATATG GTTCTGGCTT CAGCTCTGGC 
GGCATGGACT ATGGCATGGT TGGTGGGAAG GAGGCTGGAA CAGAGTCTCG CTTTAAACAG 
TGGACCTCCA TGATGGAGGG ACTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACT CGAGGAGGGT CACCGTACAA CCAGTTTGAT 
ATCATCCCGA GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCCA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT ACTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TCCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACACCGCTG 
CCCCGCCCAC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGATCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TTGCCTCGGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCCAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACGTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACCGATGATG AAGTTAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCAGCAACC 
CCAAGTGCAC CCGCCGCAGG TTGGCAGTCG CTGGAGACCG GCCAGACCCA GTCAGATCCC 
GTCGGACCTG CTTTGAATCT TTTTGGTGGG TCCACAGGGC TCGGGCAATG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCT GGCGCTTCGT TGTGGGGGCC CCCAAACTAT 
TCTTCTAGCT TGTGGGGAGT CCCAGCTGTG GAAGATCCCC ATAGGATGGG CAGCCCTGCT 
CCCTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972820.1
CDS	245..5692
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNAPSNAPNAMKPNSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_014117345.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117345.1

ORF Insert Sequence:

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GTCTGGAGCA AAAGCACACC ACCTGCTCCA 
GATAATGGTA CTTCAGCTTG GGGTGAGCCA AACGAAAGCA GTCCTGGGTG GGGCGAAATG 
GATGACACGG GAGCATCGAC CACGGGCTGG GGGAATGCAC CCTCCAACGC TCCAAATGCC 
ATGAAACCTA ATTCCAAATC TATGCAAGAC GGCTGGGGGG AGAGTGACGG TCCAGTGACG 
GGAACTCGCC ATCCCAGCTG GGAGGAGGAG GATGATGGAG GAGTCTGGAA CACCGCCGGC 
TCTCAGGGAA GTGCTTCCTC CCACAACTCA GCAAGCTGGG GACAAGGAGG AAAGAAGCAA 
ATGAAGTGCT CACTGAAAGG AGGAAACAAT GATTCATGGA TGAATCCTCT TGCCAAACAG 
TTTTCAAATA TGGGATTGCT GAGTCAAACT GAAGATAATC CAGGCAGCAA AATGGATTTG 
TCTGTAGGGA GCCTTCCAGA TAAAAAATTT GACGTGGACA AGCGAGCAAT GAATCTCGGG 
GATTTTAATG ATATCATGAG GAAGGATCGA TCTGGGTTCC GTCCACCTAA TTCCAAAGAC 
ATGGGAACCA CAGATAGTGG GCCTTATTTC GAGAAGGGTG GCAATCATGG TTTGTTTGGA 
AATAGCACAG CACAATCGAG AGGTATGCAC ACACCAGTGC AGCCACTAAA TTCTTCTCCT 
AATCTCCGGG CGCAAGTGCC TCCCCAGTTT ATTTCCCCCC AGGTTTCTGC CTCCATGCTC 
AAGCAGTTTC CCAACAGTGG CCTGAATCCA GGTCTTTTCA ATGTGGGGCC CCAGTTATCT 
CCTCAACAGA TTGCCATGCT GAGCCAGCTT CCACAAATTC CCCAGTTTCA GTTGGCATGT 
CAGCTTCTCC TGCAGCAGCA GCAACAGCAG CAGTTGCTAC AGAACCAGAG GAAGCTTTCT 
CAAGCTGTGC GCCAGCAGCA AGAGCAGCAG CTGGCTCGAA TGGTGAGCGC TCTCCAGCAA 
CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG AGGCAGCCTG GCATGAAGCA CTCACCCTCT 
CATCCTGTTG GGCCCAAGCC ACATCTGGAC AACATGGTAC CCAACGCTCT GAATGTGGGG 
CTCCCAGACC TTCAAACCAA AGGGCCAATA CCTGGATATG GTTCTGGCTT CAGCTCTGGC 
GGCATGGACT ATGGCATGGT TGGTGGGAAG GAGGCTGGAA CAGAGTCTCG CTTTAAACAG 
TGGACCTCCA TGATGGAGGG ACTGCCCTCT GTAGCCACAC AGGAAGCCAA TATGCACAAA 
AATGGCGCTA TTGTGGCCCC TGGTAAGACT CGAGGAGGGT CACCGTACAA CCAGTTTGAT 
ATCATCCCGA GTGACACACT GGGTGGCCAT ACGGGTCCTG CTGGTGATAG CTGGTTACCT 
GCCAAATCTC CACCAACAAA TAAAATCGGA AGTAAATCCA GCAATGCCAG TTGGCCTCCA 
GAATTCCAAC CAGGAGTGCC ATGGAAAGGT ATCCAAAACA TTGACCCTGA ATCTGACCCC 
TATGTCACCC CAGGAAGTGT ACTGGGGGGT ACAGCCACAT CTCCCATTGT AGATACTGAC 
CACCAACTTC TGCGGGATAA CACCACAGGG TCTAATTCTT CCCTCAACAC CTCGCTGCCT 
TCACCTGGTG CCTGGCCCTA CAGTGCCTCT GACAACTCCT TTACCAACGT TCATAGCACT 
TCAGCAAAGT TCCCTGATTA CAAATCAACA TGGTCCCCAG ATCCCATAGG ACACAACCCC 
ACTCATCTCT CCAACAAGAT GTGGAAAAAC CATATTTCCT CAAGGAACAC TACACCGCTG 
CCCCGCCCAC CTCCCGGTCT GACCAACCCC AAACCATCAT CTCCCTGGAG CAGCACAGCA 
CCCCGATCAG TCAGGGGGTG GGGGACACAG GACTCACGGC TTGCCTCGGC CTCTACCTGG 
AGTGATGGTG GTTCAGTTCG TCCCAGTTAC TGGCTGGTTC TTCACAATCT CACTCCACAG 
ATTGATGGGT CAACGTTGAG AACCATCTGC ATGCAGCATG GCCCACTGCT GACATTCCAT 
CTGAACCTAA CCCAGGGCAC TGCCCTGATC CGATACAGCA CCAAACAGGA GGCGGCCAAG 
GCCCAAACTG CACTGCACAT GTGTGTGTTG GGAAACACTA CCATCCTGGC TGAGTTTGCC 
ACCGATGATG AAGTTAGCCG CTTTCTGGCA CAAGCTCAGC CCCCTACACC TGCAGCAACC 
CCAAGTGCAC CCGCCGCAGG TTGGCAGTCG CTGGAGACCG GCCAGACCCA GTCAGATCCC 
GTCGGACCTG CTTTGAATCT TTTTGGTGGG TCCACAGGGC TCGGGCAATG GAGCAGCAGT 
GCTGGTGGCA GCAGCGGGGC CGATCTTGCT GGCGCTTCGT TGTGGGGGCC CCCAAACTAT 
TCTTCTAGCT TGTGGGGAGT CCCAGCTGTG GAAGATCCCC ATAGGATGGG CAGCCCTGCT 
CCCTTACTAC CTGGTGACCT TCTGGGAGGA GGGTCGGATT CAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6B ( XM_014117346.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972821.1 Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.

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CloneID	OCe38899
Clone ID Related Accession (Same CDS sequence)	XM_014117346.1
Accession Version	XM_014117346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	trinucleotide repeat-containing gene 6B protein isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GATTCCAAAT CTATGCAAGA CGGCTGGGGG 
GAGAGTGACG GTCCAGTGAC GGGAACTCGC CATCCCAGCT GGGAGGAGGA GGATGATGGA 
GGAGTCTGGA ACACCGCCGG CTCTCAGGGA AGTGCTTCCT CCCACAACTC AGCAAGCTGG 
GGACAAGGAG GAAAGAAGCA AATGAAGTGC TCACTGAAAG GAGGAAACAA TGATTCATGG 
ATGAATCCTC TTGCCAAACA GTTTTCAAAT ATGGGATTGC TGAGTCAAAC TGAAGATAAT 
CCAGGCAGCA AAATGGATTT GTCTGTAGGG AGCCTTCCAG ATAAAAAATT TGACGTGGAC 
AAGCGAGCAA TGAATCTCGG GGATTTTAAT GATATCATGA GGAAGGATCG ATCTGGGTTC 
CGTCCACCTA ATTCCAAAGA CATGGGAACC ACAGATAGTG GGCCTTATTT CGAGAAGGGT 
GGCAATCATG GTTTGTTTGG AAATAGCACA GCACAATCGA GAGGTATGCA CACACCAGTG 
CAGCCACTAA ATTCTTCTCC TAATCTCCGG GCGCAAGTGC CTCCCCAGTT TATTTCCCCC 
CAGGTTTCTG CCTCCATGCT CAAGCAGTTT CCCAACAGTG GCCTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGTTATC TCCTCAACAG ATTGCCATGC TGAGCCAGCT TCCACAAATT 
CCCCAGTTTC AGTTGGCATG TCAGCTTCTC CTGCAGCAGC AGCAACAGCA GCAGTTGCTA 
CAGAACCAGA GGAAGCTTTC TCAAGCTGTG CGCCAGCAGC AAGAGCAGCA GCTGGCTCGA 
ATGGTGAGCG CTCTCCAGCA ACAGCAGCAA CAGCAGCAGC AGCAGCAGCA GAGGCAGCCT 
GGCATGAAGC ACTCACCCTC TCATCCTGTT GGGCCCAAGC CACATCTGGA CAACATGGTA 
CCCAACGCTC TGAATGTGGG GCTCCCAGAC CTTCAAACCA AAGGGCCAAT ACCTGGATAT 
GGTTCTGGCT TCAGCTCTGG CGGCATGGAC TATGGCATGG TTGGTGGGAA GGAGGCTGGA 
ACAGAGTCTC GCTTTAAACA GTGGACCTCC ATGATGGAGG GACTGCCCTC TGTAGCCACA 
CAGGAAGCCA ATATGCACAA AAATGGCGCT ATTGTGGCCC CTGGTAAGAC TCGAGGAGGG 
TCACCGTACA ACCAGTTTGA TATCATCCCG AGTGACACAC TGGGTGGCCA TACGGGTCCT 
GCTGGTGATA GCTGGTTACC TGCCAAATCT CCACCAACAA ATAAAATCGG AAGTAAATCC 
AGCAATGCCA GTTGGCCTCC AGAATTCCAA CCAGGAGTGC CATGGAAAGG TATCCAAAAC 
ATTGACCCTG AATCTGACCC CTATGTCACC CCAGGAAGTG TACTGGGGGG TACAGCCACA 
TCTCCCATTG TAGATACTGA CCACCAACTT CTGCGGGATA ACACCACAGG GTCTAATTCT 
TCCCTCAACA CCTCGCTGCC TTCACCTGGT GCCTGGCCCT ACAGTGCCTC TGACAACTCC 
TTTACCAACG TTCATAGCAC TTCAGCAAAG TTCCCTGATT ACAAATCAAC ATGGTCCCCA 
GATCCCATAG GACACAACCC CACTCATCTC TCCAACAAGA TGTGGAAAAA CCATATTTCC 
TCAAGGAACA CTACACCGCT GCCCCGCCCA CCTCCCGGTC TGACCAACCC CAAACCATCA 
TCTCCCTGGA GCAGCACAGC ACCCCGATCA GTCAGGGGGT GGGGGACACA GGACTCACGG 
CTTGCCTCGG CCTCTACCTG GAGTGATGGT GGTTCAGTTC GTCCCAGTTA CTGGCTGGTT 
CTTCACAATC TCACTCCACA GATTGATGGG TCAACGTTGA GAACCATCTG CATGCAGCAT 
GGCCCACTGC TGACATTCCA TCTGAACCTA ACCCAGGGCA CTGCCCTGAT CCGATACAGC 
ACCAAACAGG AGGCGGCCAA GGCCCAAACT GCACTGCACA TGTGTGTGTT GGGAAACACT 
ACCATCCTGG CTGAGTTTGC CACCGATGAT GAAGTTAGCC GCTTTCTGGC ACAAGCTCAG 
CCCCCTACAC CTGCAGCAAC CCCAAGTGCA CCCGCCGCAG GTTGGCAGTC GCTGGAGACC 
GGCCAGACCC AGTCAGATCC CGTCGGACCT GCTTTGAATC TTTTTGGTGG GTCCACAGGG 
CTCGGGCAAT GGAGCAGCAG TGCTGGTGGC AGCAGCGGGG CCGATCTTGC TGGCGCTTCG 
TTGTGGGGGC CCCCAAACTA TTCTTCTAGC TTGTGGGGAG TCCCAGCTGT GGAAGATCCC 
CATAGGATGG GCAGCCCTGC TCCCTTACTA CCTGGTGACC TTCTGGGAGG AGGGTCGGAT 
TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972821.1
CDS	245..5533
Translation	MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPSTAPGANSNNAQVTGTLLQSDSGTAPESTLGGAAASNYANSTWGPGASSNNGASPNPIHTWDKVIVDGSDVEEWPCIASKDTESSSENTTDNNSASNPGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKTKSVQSSNSTTESNNGLGNWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGNSRKGALETDTSNSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNASRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTASGQSNSGNNGNNGKDREDSWKGASVQKSSGSKNDSWDNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNTGGWNDYKNNNSSNWGGGRPDEKTSSSWNENSSKDQGWGGGRQPNQGWTSGKNGWGEEVDQAKNSSWESSASKPVSGWGEGGQNEIGTWGNSGNASLASKGGWEDCKRSPAWTETGRQPNSWHKQHQQQQQPQQPPPPQPEASGSWGGPPPPPPGNGRPSNSNWSSGPQPVTPKDEEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTSKSASDSKSMQDGWGESDGPVTGTRHPSWEEEDDGGVWNTAGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPGSKMDLSVGSLPDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGNHGLFGNSTAQSRGMHTPVQPLNSSPNLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKLSQAVRQQQEQQLARMVSALQQQQQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPSDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQTQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPAVEDPHRMGSPAPLLPGDLLGGGSDSI

Target ORF information:

RefSeq Version	XM_014117346.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris trinucleotide repeat containing 6B (TNRC6B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117346.1

ORF Insert Sequence:

ATGCAAACCA ATGAGGGAGA AGTGTCGGAA GAAAGCAGTT CCAAGGTGGA ACAGGAGGAT 
TTTGTAATGG AAGGGCATGG CAAGACTCCA CCTCCTGGTG AAGAAAGCAA ACAAGAGAAG 
GAGCAAGAAA GGGAAGAACA GTTAATGGAA GACAAGAAAA GGAAGAAAGA GGATAAAAAG 
AAAAAAGAAG CCACTCAGAA GGTCACGGAA CAAAAAACCA AAGTGCCCGA AGTGACGAAA 
CCAAGTTTAA GCCAACCAAC GGCCGCCAGC CCAATTGGCA GCTCTCCATC GCCACCAGTC 
AATGGTGGCA ACAATGCCAA AAGGGTGGCA GTGCCGAACG GACAACCGCC AAGCGCCGCC 
CGCTACATGC CTCGGGAGGT GCCGCCGCGA TTCCGTTGCC AGCAGGACCA CAAAGTGTTA 
CTAAAACGTG GGCAGCCCCC TCCGCCGTCC TGCATGCTCC TTGGGGGCGG AGCAGGGCCT 
CCTCCCTCCA CAGCACCTGG AGCAAACTCA AACAACGCAC AAGTGACAGG AACGCTGCTG 
CAGAGTGACA GTGGGACTGC ACCAGAGTCA ACCCTTGGAG GTGCTGCTGC TTCAAATTAT 
GCAAATTCCA CTTGGGGCCC GGGAGCCTCC TCCAACAACG GCGCCTCCCC CAACCCAATT 
CACACCTGGG ACAAGGTGAT TGTAGACGGG TCTGACGTGG AAGAGTGGCC TTGTATTGCC 
AGCAAAGACA CTGAGTCTTC TTCCGAAAAC ACCACCGATA ACAACAGTGC CTCGAACCCT 
GGCTCTGAGA AGAGCACTCT GCCAGGAAGC ACCACTAGTA ACAAAGGAAA AGGGAGCCAG 
TGCCAGTCTG CAAGTTCTGG GAACGAATGT AATCTTGGGG TCTGGAAATC TGACCCTAAG 
ACTAAATCTG TTCAATCTTC CAACTCTACA ACAGAGAGCA ACAATGGACT AGGAAATTGG 
AGGAATGTGA GTGGTCAGGA TAGAATTGGA CCTGGCTCTG GCTTCAGCAA CTTTAACCCA 
AATAGCAACC CATCTGCCTG GCCAGCGCTG GTCCAAGAAG GAAATTCTAG GAAAGGGGCA 
TTGGAAACGG ATACTAGTAA TTCCAGTGCA CAGGTTAGCA CAGTAGGTCA GGCATCCAGG 
GAACAGCAGT CAAAGATGGA AAATGCGGGT GTTAATTTTG TTGTCTCTGG CAGAGAACAG 
GCTCAGATTC ATAACACTGA TGGACCAAAA AATGGAAACA CTAACTCCTT GAACTTAAGT 
TCACCAAACC CCATGGAGAA TAAGGGAATG CCCTTTGGAA TGGGCTTGGG GAACGCCTCC 
AGGAGCACTG ATGCCCCTTC ACAAAGCACT GGAGATCGAA AGACTGGGAG TGTTGGATCT 
TGGGGTGCAG CTAGGGGGCC TTCTGGAACT GACACAGCCT CTGGACAAAG CAATTCTGGA 
AACAATGGGA ACAATGGAAA AGATAGAGAG GACTCCTGGA AAGGAGCTTC TGTTCAGAAA 
TCATCTGGGT CAAAAAATGA CTCTTGGGAC AATAACAGGT CTACGGGTGG GTCCTGGAAC 
TTTGGCCCTC AGGACTCTAA TGACAACAAA TGGGGTGAAG GGAACAAAAT GACATCTGGG 
GTCTCTCAGG GAGAATGGAA ACAGCCGACT GGGTCTGATG AGTTGAAAAT TGGAGAATGG 
AGTGGTCCAA ACCAACCAAA TTCTAGCACT GGAGCATGGG ACAATCAAAA GGGCCACCCC 
CTCCCTGAAA ACCAAGGCAA TGCCCAGGCT CCCTGTTGGG GAAGATCTTC CAGCTCCACA 
GGAAGTGAAG TTGGAGGTCA AAGCACTGGA AGCAACCACA AAGCAGGAAG TAGTGACAGT 
CATAATTCTG GCCGTCGGTC ATACCGGCCC ACACATCCTG ATTGTCAGGC TGTCTTACAG 
ACTCTTTTGA GCCGAACTGA TTTGGACCCC AGGGTGCTCT CAAACACTGG CTGGGGCCAA 
ACTCAAATTA AGCAGGACAC TGTGTGGGAT ATTGAAGAGG TGCCAAGGCC TGAGGGGAAA 
TCGGACAAAG GAACTGAGGG GTGGGAGAGC GCTGCCACAC AGACCAAGAA CTCAGGGGGC 
TGGGGAGATG CACCCAGCCA AAGCAATCAA ATGAAGTCTG GATGGGGGGA GCTCTCAGCC 
TCTACAGAGT GGAAAGACCC CAAGAACACA GGAGGCTGGA ATGACTATAA GAACAACAAC 
TCTTCCAACT GGGGAGGCGG ACGACCAGAT GAAAAGACAT CTTCCTCTTG GAATGAGAAC 
TCCAGCAAGG ATCAGGGGTG GGGAGGGGGA CGCCAGCCCA ATCAAGGATG GACTTCTGGG 
AAGAATGGTT GGGGAGAGGA AGTTGATCAG GCGAAAAACA GCAGTTGGGA AAGTTCTGCA 
AGCAAACCTG TGTCTGGATG GGGAGAAGGA GGGCAGAATG AAATTGGAAC TTGGGGGAAC 
AGCGGGAATG CAAGCCTAGC TTCCAAAGGT GGGTGGGAAG ATTGCAAAAG GTCTCCAGCG 
TGGACTGAGA CTGGCAGACA GCCCAATTCC TGGCATAAAC AGCACCAACA GCAGCAGCAG 
CCACAGCAGC CACCGCCACC ACAACCAGAG GCTTCTGGTT CATGGGGAGG CCCCCCACCA 
CCACCTCCAG GCAACGGACG ACCTTCCAAT TCCAACTGGA GCAGTGGGCC ACAGCCAGTA 
ACCCCTAAGG ACGAGGAACC CAGTGGTTGG GAAGAGCCAT CCCCACAGTC AATTAGTCGG 
AAAATGGACA TCGATGATGG CACTTCAGCA TGGGGAGACC CTAACAGCTA TAACTACAAG 
AATGTGAATC TGTGGGATAA GAATTCCCAA GGGGGCCCAG CACCTCGAGA ACCAAACCTG 
CCTACCCCGA TGACCAGTAA ATCAGCATCA GATTCCAAAT CTATGCAAGA CGGCTGGGGG 
GAGAGTGACG GTCCAGTGAC GGGAACTCGC CATCCCAGCT GGGAGGAGGA GGATGATGGA 
GGAGTCTGGA ACACCGCCGG CTCTCAGGGA AGTGCTTCCT CCCACAACTC AGCAAGCTGG 
GGACAAGGAG GAAAGAAGCA AATGAAGTGC TCACTGAAAG GAGGAAACAA TGATTCATGG 
ATGAATCCTC TTGCCAAACA GTTTTCAAAT ATGGGATTGC TGAGTCAAAC TGAAGATAAT 
CCAGGCAGCA AAATGGATTT GTCTGTAGGG AGCCTTCCAG ATAAAAAATT TGACGTGGAC 
AAGCGAGCAA TGAATCTCGG GGATTTTAAT GATATCATGA GGAAGGATCG ATCTGGGTTC 
CGTCCACCTA ATTCCAAAGA CATGGGAACC ACAGATAGTG GGCCTTATTT CGAGAAGGGT 
GGCAATCATG GTTTGTTTGG AAATAGCACA GCACAATCGA GAGGTATGCA CACACCAGTG 
CAGCCACTAA ATTCTTCTCC TAATCTCCGG GCGCAAGTGC CTCCCCAGTT TATTTCCCCC 
CAGGTTTCTG CCTCCATGCT CAAGCAGTTT CCCAACAGTG GCCTGAATCC AGGTCTTTTC 
AATGTGGGGC CCCAGTTATC TCCTCAACAG ATTGCCATGC TGAGCCAGCT TCCACAAATT 
CCCCAGTTTC AGTTGGCATG TCAGCTTCTC CTGCAGCAGC AGCAACAGCA GCAGTTGCTA 
CAGAACCAGA GGAAGCTTTC TCAAGCTGTG CGCCAGCAGC AAGAGCAGCA GCTGGCTCGA 
ATGGTGAGCG CTCTCCAGCA ACAGCAGCAA CAGCAGCAGC AGCAGCAGCA GAGGCAGCCT 
GGCATGAAGC ACTCACCCTC TCATCCTGTT GGGCCCAAGC CACATCTGGA CAACATGGTA 
CCCAACGCTC TGAATGTGGG GCTCCCAGAC CTTCAAACCA AAGGGCCAAT ACCTGGATAT 
GGTTCTGGCT TCAGCTCTGG CGGCATGGAC TATGGCATGG TTGGTGGGAA GGAGGCTGGA 
ACAGAGTCTC GCTTTAAACA GTGGACCTCC ATGATGGAGG GACTGCCCTC TGTAGCCACA 
CAGGAAGCCA ATATGCACAA AAATGGCGCT ATTGTGGCCC CTGGTAAGAC TCGAGGAGGG 
TCACCGTACA ACCAGTTTGA TATCATCCCG AGTGACACAC TGGGTGGCCA TACGGGTCCT 
GCTGGTGATA GCTGGTTACC TGCCAAATCT CCACCAACAA ATAAAATCGG AAGTAAATCC 
AGCAATGCCA GTTGGCCTCC AGAATTCCAA CCAGGAGTGC CATGGAAAGG TATCCAAAAC 
ATTGACCCTG AATCTGACCC CTATGTCACC CCAGGAAGTG TACTGGGGGG TACAGCCACA 
TCTCCCATTG TAGATACTGA CCACCAACTT CTGCGGGATA ACACCACAGG GTCTAATTCT 
TCCCTCAACA CCTCGCTGCC TTCACCTGGT GCCTGGCCCT ACAGTGCCTC TGACAACTCC 
TTTACCAACG TTCATAGCAC TTCAGCAAAG TTCCCTGATT ACAAATCAAC ATGGTCCCCA 
GATCCCATAG GACACAACCC CACTCATCTC TCCAACAAGA TGTGGAAAAA CCATATTTCC 
TCAAGGAACA CTACACCGCT GCCCCGCCCA CCTCCCGGTC TGACCAACCC CAAACCATCA 
TCTCCCTGGA GCAGCACAGC ACCCCGATCA GTCAGGGGGT GGGGGACACA GGACTCACGG 
CTTGCCTCGG CCTCTACCTG GAGTGATGGT GGTTCAGTTC GTCCCAGTTA CTGGCTGGTT 
CTTCACAATC TCACTCCACA GATTGATGGG TCAACGTTGA GAACCATCTG CATGCAGCAT 
GGCCCACTGC TGACATTCCA TCTGAACCTA ACCCAGGGCA CTGCCCTGAT CCGATACAGC 
ACCAAACAGG AGGCGGCCAA GGCCCAAACT GCACTGCACA TGTGTGTGTT GGGAAACACT 
ACCATCCTGG CTGAGTTTGC CACCGATGAT GAAGTTAGCC GCTTTCTGGC ACAAGCTCAG 
CCCCCTACAC CTGCAGCAAC CCCAAGTGCA CCCGCCGCAG GTTGGCAGTC GCTGGAGACC 
GGCCAGACCC AGTCAGATCC CGTCGGACCT GCTTTGAATC TTTTTGGTGG GTCCACAGGG 
CTCGGGCAAT GGAGCAGCAG TGCTGGTGGC AGCAGCGGGG CCGATCTTGC TGGCGCTTCG 
TTGTGGGGGC CCCCAAACTA TTCTTCTAGC TTGTGGGGAG TCCCAGCTGT GGAAGATCCC 
CATAGGATGG GCAGCCCTGC TCCCTTACTA CCTGGTGACC TTCTGGGAGG AGGGTCGGAT 
TCAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TNRC6B
Entrez Gene ID	481240
Full Name	trinucleotide repeat containing 6B
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

	TNRC6B	XP_001101111.2
Canis lupus familiaris (dog)	TNRC6B	XP_005625909.1
Bos taurus (cattle)	TNRC6B	NP_001179584.1
Gallus gallus (chicken)	TNRC6B	XP_004937779.1
Danio rerio (zebrafish)	tnrc6b	XP_002663990.3
Xenopus tropicalis (tropical clawed frog)	tnrc6b	XP_002934255.2
Homo sapiens (human)	TNRC6B	NP_001155973.1
Pan troglodytes (chimpanzee)	TNRC6B	XP_003317299.1
Mus musculus (house mouse)	Tnrc6b	NP_659061.2
Rattus norvegicus (Norway rat)	Tnrc6b	NP_620200.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TNRC6B cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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