MID2 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005641647.2	XP_005641704.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_014111777.1	XP_013967252.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_003435641.3	XP_003435689.1	probable E3 ubiquitin-protein ligase MID2 isoform X3
XM_014111778.2	XP_013967253.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_014111776.2	XP_013967251.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_022416161.1	XP_022271869.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_014111779.1	XP_013967254.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_005641647.3	XP_005641704.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_014111779.2	XP_013967254.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_014111776.1	XP_013967251.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_014111777.2	XP_013967252.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_003435641.4	XP_003435689.1	probable E3 ubiquitin-protein ligase MID2 isoform X3
XM_014111778.1	XP_013967253.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_022416162.1	XP_022271870.1	probable E3 ubiquitin-protein ligase MID2 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe32740	XM_005641647.3 Latest version!	Canis lupus familiaris midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe32741	XM_003435641.4 Latest version!	Canis lupus familiaris midline 2 (MID2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe285411	XM_022416162.1 Latest version!	Canis lupus familiaris midline 2 (MID2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe45682	XM_014111777.2 Latest version!	Canis lupus familiaris midline 2 (MID2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe45682	XM_014111776.2 Latest version!	Canis lupus familiaris midline 2 (MID2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe45682	XM_014111779.2 Latest version!	Canis lupus familiaris midline 2 (MID2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe45682	XM_014111778.2 Latest version!	Canis lupus familiaris midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe45682	XM_022416161.1 Latest version!	Canis lupus familiaris midline 2 (MID2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OCe32740	XM_005641647.2	Canis lupus familiaris midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe45682	XM_014111779.1	Canis lupus familiaris midline 2 (MID2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe45682	XM_014111776.1	Canis lupus familiaris midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe45682	XM_014111777.1	Canis lupus familiaris midline 2 (MID2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe32741	XM_003435641.3	Canis lupus familiaris midline 2 (MID2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe45682	XM_014111778.1	Canis lupus familiaris midline 2 (MID2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

MID2 ( XM_005641647.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641704.1 Canis lupus familiaris midline 2 (MID2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe32740
Clone ID Related Accession (Same CDS sequence)	XM_005641647.2 , XM_005641647.3
Accession Version	XM_005641647.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCAGAGCCTC TGTGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641704.1
CDS	153..2360
Translation	MGESRASVVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_005641647.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641647.2

ORF Insert Sequence:

ATGGGTGAAA GCAGAGCCTC TGTGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_005641647.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641704.1 Canis lupus familiaris midline 2 (MID2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe32740
Clone ID Related Accession (Same CDS sequence)	XM_005641647.2 , XM_005641647.3
Accession Version	XM_005641647.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641647.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCAGAGCCTC TGTGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641704.1
CDS	119..2326
Translation	MGESRASVVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_005641647.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641647.3

ORF Insert Sequence:

ATGGGTGAAA GCAGAGCCTC TGTGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_003435641.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003435689.1 Canis lupus familiaris midline 2 (MID2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe32741
Clone ID Related Accession (Same CDS sequence)	XM_003435641.3 , XM_003435641.4
Accession Version	XM_003435641.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003435641.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCAGAGCCTC TGTGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AACAGAACCA CTACACAGTG CATGGACTTC AGAGCGGGAC CCGCTACATC 
TTCATTGTTA AAGCCATAAA CCAAGCAGGC AGCCGGAACA GTGAACCCAC CCGACTAAAA 
ACAAATAGCC AACCCTTTAA ATTGGATCCG AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGGT TGCAGATGGA GAAGGATGAA AGCTCTCTGA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGCA GCACAGGGTG CTATGGGGCA GCAGGGAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTAGTTAT GGGTTCCTGC ACCTGGTATG CAATTGGTAT TGCCTACAAG 
TCCGCTCCCA AGAACGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGATGC 
AATAGTAACT TTGTAGTGAG ACATAACAAC AAGGAAATGT TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCGTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTCATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435689.1
CDS	148..2265
Translation	MGESRASVVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_003435641.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003435641.3

ORF Insert Sequence:

ATGGGTGAAA GCAGAGCCTC TGTGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AACAGAACCA CTACACAGTG CATGGACTTC AGAGCGGGAC CCGCTACATC 
TTCATTGTTA AAGCCATAAA CCAAGCAGGC AGCCGGAACA GTGAACCCAC CCGACTAAAA 
ACAAATAGCC AACCCTTTAA ATTGGATCCG AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGGT TGCAGATGGA GAAGGATGAA AGCTCTCTGA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGCA GCACAGGGTG CTATGGGGCA GCAGGGAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTAGTTAT GGGTTCCTGC ACCTGGTATG CAATTGGTAT TGCCTACAAG 
TCCGCTCCCA AGAACGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGATGC 
AATAGTAACT TTGTAGTGAG ACATAACAAC AAGGAAATGT TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCGTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTCATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_003435641.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003435689.1 Canis lupus familiaris midline 2 (MID2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe32741
Clone ID Related Accession (Same CDS sequence)	XM_003435641.3 , XM_003435641.4
Accession Version	XM_003435641.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003435641.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCAGAGCCTC TGTGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AACAGAACCA CTACACAGTG CATGGACTTC AGAGCGGGAC CCGCTACATC 
TTCATTGTTA AAGCCATAAA CCAAGCAGGC AGCCGGAACA GTGAACCCAC CCGACTAAAA 
ACAAATAGCC AACCCTTTAA ATTGGATCCG AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGGT TGCAGATGGA GAAGGATGAA AGCTCTCTGA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGCA GCACAGGGTG CTATGGGGCA GCAGGGAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTAGTTAT GGGTTCCTGC ACCTGGTATG CAATTGGTAT TGCCTACAAG 
TCCGCTCCCA AGAACGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGATGC 
AATAGTAACT TTGTAGTGAG ACATAACAAC AAGGAAATGT TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCGTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTCATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435689.1
CDS	118..2235
Translation	MGESRASVVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_003435641.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003435641.4

ORF Insert Sequence:

ATGGGTGAAA GCAGAGCCTC TGTGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AACAGAACCA CTACACAGTG CATGGACTTC AGAGCGGGAC CCGCTACATC 
TTCATTGTTA AAGCCATAAA CCAAGCAGGC AGCCGGAACA GTGAACCCAC CCGACTAAAA 
ACAAATAGCC AACCCTTTAA ATTGGATCCG AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGGT TGCAGATGGA GAAGGATGAA AGCTCTCTGA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGCA GCACAGGGTG CTATGGGGCA GCAGGGAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTAGTTAT GGGTTCCTGC ACCTGGTATG CAATTGGTAT TGCCTACAAG 
TCCGCTCCCA AGAACGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGATGC 
AATAGTAACT TTGTAGTGAG ACATAACAAC AAGGAAATGT TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCGTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTCATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_022416162.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271870.1 Canis lupus familiaris midline 2 (MID2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe285411
Clone ID Related Accession (Same CDS sequence)	XM_022416162.1
Accession Version	XM_022416162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCAGAGCCTC TGTGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTTA TGAAGCTGAG AAAGTTGGCA 
CAGCAGGTTG CTAATTGTCG ACAGTGTCTT GAACGGTCAA CAGTCCTCAT CAACCAGGCT 
GAGCATATCC TGAAAGAAAA TGACCAGGCA CGGTTTCTCC AATCTGCAAA GAATATTGCT 
GAGAGGGTCG CTATGGCAAC AGCGTCTTCT CAAGTTCTGA TTCCAGACAT CAATTTTAAT 
GATGCCTTTG AAAACTTTGC TTTAGATTTT TCCAGAGAAA AGAAACTGCT GGAGGGGCTA 
GATTATTTAA CAGCCCCAAA CCCACCATGT ATCCGAGAGG AACTCTGTAC TGCTTCCCAT 
GACACCATTA CAGTCCACTG GATCTCGGAC GATGAGTTCA GCATCAGCTC CTATGAGCTT 
CAGTACACCA TATTCACTGG CCAGGCTAAC TTCATCAGTA AGTCATGGTG TAGTTGGGGC 
CTGTGGCCAG AGATAAGGAA ATGTAAGGAA GCAGTAAGCT GCTCAAGATT GGCCGGGGCG 
CCACGAGGCC TGTACAATTC AGTGGATAGC TGGATGATTG TGCCCAACAT TAAACAGAAC 
CACTACACAG TGCATGGACT TCAGAGCGGG ACCCGCTACA TCTTCATTGT TAAAGCCATA 
AACCAAGCAG GCAGCCGGAA CAGTGAACCC ACCCGACTAA AAACAAATAG CCAACCCTTT 
AAATTGGATC CGAAAATGAC TCACAAGAAG TTGAAGATCT CCAATGATGG GTTGCAGATG 
GAGAAGGATG AAAGCTCTCT GAAGAAGAGC CATACCCCAG AGAGGTTTAG CAGCACAGGG 
TGCTATGGGG CAGCAGGGAA TATATTCATT GACAGTGGCT GCCACTACTG GGAGGTAGTT 
ATGGGTTCCT GCACCTGGTA TGCAATTGGT ATTGCCTACA AGTCCGCTCC CAAGAACGAA 
TGGATTGGCA AGAATGCCTC CTCATGGGTC TTCTCTCGAT GCAATAGTAA CTTTGTAGTG 
AGACATAACA ACAAGGAAAT GTTGGTGGAT GTGCCCCCAC AGTTGAAGCG TCTGGGTGTC 
CTTCTGGATT ATGACAACAA CATGCTGTCG TTCTATGACC CAGCTAACTC TCTCCATCTT 
CATACTTTTG ATGTGACCTT CATTCTTCCA GTTTGTCCAA CATTCACAAT CTGGAACAAA 
TCCCTCATGA TCCTGTCTGG CTTGCCTGCC CCAGATTTTA TTGATTACCC TGAGCGGCAG 
GAATGCAACT GCAGGCCTCA AGAATCCCCT TATGTTTCTG GGATGAAAGC TTGCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271870.1
CDS	120..2219
Translation	MGESRASVVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_022416162.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416162.1

ORF Insert Sequence:

ATGGGTGAAA GCAGAGCCTC TGTGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTTA TGAAGCTGAG AAAGTTGGCA 
CAGCAGGTTG CTAATTGTCG ACAGTGTCTT GAACGGTCAA CAGTCCTCAT CAACCAGGCT 
GAGCATATCC TGAAAGAAAA TGACCAGGCA CGGTTTCTCC AATCTGCAAA GAATATTGCT 
GAGAGGGTCG CTATGGCAAC AGCGTCTTCT CAAGTTCTGA TTCCAGACAT CAATTTTAAT 
GATGCCTTTG AAAACTTTGC TTTAGATTTT TCCAGAGAAA AGAAACTGCT GGAGGGGCTA 
GATTATTTAA CAGCCCCAAA CCCACCATGT ATCCGAGAGG AACTCTGTAC TGCTTCCCAT 
GACACCATTA CAGTCCACTG GATCTCGGAC GATGAGTTCA GCATCAGCTC CTATGAGCTT 
CAGTACACCA TATTCACTGG CCAGGCTAAC TTCATCAGTA AGTCATGGTG TAGTTGGGGC 
CTGTGGCCAG AGATAAGGAA ATGTAAGGAA GCAGTAAGCT GCTCAAGATT GGCCGGGGCG 
CCACGAGGCC TGTACAATTC AGTGGATAGC TGGATGATTG TGCCCAACAT TAAACAGAAC 
CACTACACAG TGCATGGACT TCAGAGCGGG ACCCGCTACA TCTTCATTGT TAAAGCCATA 
AACCAAGCAG GCAGCCGGAA CAGTGAACCC ACCCGACTAA AAACAAATAG CCAACCCTTT 
AAATTGGATC CGAAAATGAC TCACAAGAAG TTGAAGATCT CCAATGATGG GTTGCAGATG 
GAGAAGGATG AAAGCTCTCT GAAGAAGAGC CATACCCCAG AGAGGTTTAG CAGCACAGGG 
TGCTATGGGG CAGCAGGGAA TATATTCATT GACAGTGGCT GCCACTACTG GGAGGTAGTT 
ATGGGTTCCT GCACCTGGTA TGCAATTGGT ATTGCCTACA AGTCCGCTCC CAAGAACGAA 
TGGATTGGCA AGAATGCCTC CTCATGGGTC TTCTCTCGAT GCAATAGTAA CTTTGTAGTG 
AGACATAACA ACAAGGAAAT GTTGGTGGAT GTGCCCCCAC AGTTGAAGCG TCTGGGTGTC 
CTTCTGGATT ATGACAACAA CATGCTGTCG TTCTATGACC CAGCTAACTC TCTCCATCTT 
CATACTTTTG ATGTGACCTT CATTCTTCCA GTTTGTCCAA CATTCACAAT CTGGAACAAA 
TCCCTCATGA TCCTGTCTGG CTTGCCTGCC CCAGATTTTA TTGATTACCC TGAGCGGCAG 
GAATGCAACT GCAGGCCTCA AGAATCCCCT TATGTTTCTG GGATGAAAGC TTGCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_014111777.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967252.1 Canis lupus familiaris midline 2 (MID2), transcript variant X3, mRNA.

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CloneID	OCe45682
Clone ID Related Accession (Same CDS sequence)	XM_014111777.1 , XM_014111778.2 , XM_014111776.2 , XM_022416161.1 , XM_014111779.1 , XM_014111779.2 , XM_014111776.1 , XM_014111777.2 , XM_014111778.1
Accession Version	XM_014111777.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967252.1
CDS	75..2222
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_014111777.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111777.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_014111778.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967253.1 Canis lupus familiaris midline 2 (MID2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe45682
Clone ID Related Accession (Same CDS sequence)	XM_014111777.1 , XM_014111778.2 , XM_014111776.2 , XM_022416161.1 , XM_014111779.1 , XM_014111779.2 , XM_014111776.1 , XM_014111777.2 , XM_014111778.1
Accession Version	XM_014111778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967253.1
CDS	396..2543
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_014111778.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111778.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_014111776.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967251.1 Canis lupus familiaris midline 2 (MID2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe45682
Clone ID Related Accession (Same CDS sequence)	XM_014111777.1 , XM_014111778.2 , XM_014111776.2 , XM_022416161.1 , XM_014111779.1 , XM_014111779.2 , XM_014111776.1 , XM_014111777.2 , XM_014111778.1
Accession Version	XM_014111776.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967251.1
CDS	141..2288
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_014111776.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111776.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_022416161.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271869.1 Canis lupus familiaris midline 2 (MID2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe45682
Clone ID Related Accession (Same CDS sequence)	XM_014111777.1 , XM_014111778.2 , XM_014111776.2 , XM_022416161.1 , XM_014111779.1 , XM_014111779.2 , XM_014111776.1 , XM_014111777.2 , XM_014111778.1
Accession Version	XM_022416161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271869.1
CDS	675..2822
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_022416161.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416161.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_014111779.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967254.1 Canis lupus familiaris midline 2 (MID2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe45682
Clone ID Related Accession (Same CDS sequence)	XM_014111777.1 , XM_014111778.2 , XM_014111776.2 , XM_022416161.1 , XM_014111779.1 , XM_014111779.2 , XM_014111776.1 , XM_014111777.2 , XM_014111778.1
Accession Version	XM_014111779.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967254.1
CDS	137..2284
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_014111779.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111779.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_014111779.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967254.1 Canis lupus familiaris midline 2 (MID2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe45682
Clone ID Related Accession (Same CDS sequence)	XM_014111777.1 , XM_014111778.2 , XM_014111776.2 , XM_022416161.1 , XM_014111779.1 , XM_014111779.2 , XM_014111776.1 , XM_014111777.2 , XM_014111778.1
Accession Version	XM_014111779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967254.1
CDS	253..2400
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_014111779.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111779.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_014111776.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967251.1 Canis lupus familiaris midline 2 (MID2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe45682
Clone ID Related Accession (Same CDS sequence)	XM_014111777.1 , XM_014111778.2 , XM_014111776.2 , XM_022416161.1 , XM_014111779.1 , XM_014111779.2 , XM_014111776.1 , XM_014111777.2 , XM_014111778.1
Accession Version	XM_014111776.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967251.1
CDS	148..2295
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_014111776.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111776.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_014111777.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967252.1 Canis lupus familiaris midline 2 (MID2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe45682
Clone ID Related Accession (Same CDS sequence)	XM_014111777.1 , XM_014111778.2 , XM_014111776.2 , XM_022416161.1 , XM_014111779.1 , XM_014111779.2 , XM_014111776.1 , XM_014111777.2 , XM_014111778.1
Accession Version	XM_014111777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967252.1
CDS	154..2301
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_014111777.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111777.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_014111778.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967253.1 Canis lupus familiaris midline 2 (MID2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe45682
Clone ID Related Accession (Same CDS sequence)	XM_014111777.1 , XM_014111778.2 , XM_014111776.2 , XM_022416161.1 , XM_014111779.1 , XM_014111779.2 , XM_014111776.1 , XM_014111777.2 , XM_014111778.1
Accession Version	XM_014111778.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967253.1
CDS	218..2365
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPCIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSCTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_014111778.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris midline 2 (MID2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111778.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TGGAGTTGTT CGAAGACCCC 
CTTCTGCTCC CTTGCGCTCA CAGCCTCTGC TTTAGCTGTG CCCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATCG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC CAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACT GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC CAGGGATGCA GTGAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CCGTGCCAGA CACCCACCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAC 
GAGAAAGTGA ACATGTATTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGACGTC ACCGAGACCA TCAGGTTGCG TCCCTGAATG ACCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTGAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAACAA GTCGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAGCTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGTCGAC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTCCAA TCTGCAAAGA ATATTGCTGA GAGGGTCGCT 
ATGGCAACAG CGTCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTGG AGGGGCTAGA TTATTTAACA 
GCCCCAAACC CACCATGTAT CCGAGAGGAA CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGACGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AACAGAACCA CTACACAGTG 
CATGGACTTC AGAGCGGGAC CCGCTACATC TTCATTGTTA AAGCCATAAA CCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CCGACTAAAA ACAAATAGCC AACCCTTTAA ATTGGATCCG 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGGT TGCAGATGGA GAAGGATGAA 
AGCTCTCTGA AGAAGAGCCA TACCCCAGAG AGGTTTAGCA GCACAGGGTG CTATGGGGCA 
GCAGGGAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTAGTTAT GGGTTCCTGC 
ACCTGGTATG CAATTGGTAT TGCCTACAAG TCCGCTCCCA AGAACGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGATGC AATAGTAACT TTGTAGTGAG ACATAACAAC 
AAGGAAATGT TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCGTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTCATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MID2
Entrez Gene ID	481012
Full Name	midline 2
General protein information	Preferred Names midline 2 Names midline-2 probable E3 ubiquitin-protein ligase MID2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Pan troglodytes (chimpanzee)	MID2	XP_521213.3
Gallus gallus (chicken)	MID2	XP_004940642.1
Rattus norvegicus (Norway rat)	Mid2	NP_001178818.1
Homo sapiens (human)	MID2	NP_036348.2
Canis lupus familiaris (dog)	MID2	XP_005641704.1
Mus musculus (house mouse)	Mid2	NP_035975.1
Xenopus tropicalis (tropical clawed frog)	LOC100497646	XP_002936823.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MID2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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