TOP2A cDNA ORF clone, Canis lupus familiaris(Dog)

The following TOP2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe11750 XM_005624532.2
Latest version!
Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe11751 XM_537646.5
Latest version!
Canis lupus familiaris DNA topoisomerase II alpha (TOP2A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe11752 XM_005624533.2
Latest version!
Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OCe11751 XM_537646.4 Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe11750
    Clone ID Related Accession (Same CDS sequence) XM_005624532.2
    Accession Version XM_005624532.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA topoisomerase 2-alpha isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCTAG TAGTGAAATA TTACCGGCTA CACTGGCTTC TCTGGAGTCC GGTAAATGAA 
    AACATGCAAA TCAGCAAAAC AAAGAAAAAT GAAGATGCTA AGAAAAGACT GTCTATTGAA
    AGGATCTATC AAAAGAAAAC CCAACTGGAA CATATTTTGC TCCGCCCGGA CACCTACATT
    GGCTCTGTGG AATTAGTGAC CCAGCAAATG TGGGTTTACG ATGAAGATAT TGGCATGAAC
    TATAGGGAAG TCACCTTTGT CCCTGGGTTG TACAAAATCT TTGATGAGAT TCTGGTCAAT
    GCTGCAGACA ACAAACAAAG GGACCCAAAA ATGTCTTGTA TTAGAGTCAC AATTGACCCG
    GAAAACAATT TAATTAGTAT ATGGAATAAT GGGAAAGGTA TTCCTGTTGT TGAACACAAA
    GTTGAGAAGA TGTATGTCCC AGCACTCATC TTTGGACAGC TCCTAACTTC TAGTAACTAT
    GATGATGATG AAAAGAAAGT AACAGGTGGT CGAAATGGCT ATGGGGCCAA ATTGTGTAAC
    ATATTCAGTA CCAAGTTTAC TGTGGAAACA GCCAGTAGAG AATACAAGAA AATGTTCAAA
    CAGACGTGGA TGGATAACAT GGGAAGGGCT GGTGAGATGG AACTCAGGCC ATTTAATGGG
    GAAGATTATA CATGTATTAC CTTCCAGCCT GACTTGTCCA AGTTTAAAAT GCAAAGCCTA
    GATAAAGACA TTGTTGCACT GATGGTTAGA AGAGCATACG ATATTGCTGG ATCCACTAAA
    GATGTCAAAG TCTTTCTCAA TGGAAATAAA CTACCAGTAA AAGGATTCCG TAGCTACGTA
    GATATGTATC TGAAGGATAA GGTAGATGAA ACTGGCAATC CATTGAAAAT TATCCATGAA
    CAAGTAAACC CCAGGTGGGA AGTATGTTTA ACAATGAGTG AAAAAGGCTT TCAGCAAATT
    AGCTTCGTCA ACAGCATTGC TACCTCCAAG GGTGGCAGAC ATGTTGATTA TATAGCTGAT
    CAGATTGTGA CTAAACTTGT TGATGTTGTG AAGAAGAAGA ACAAGGGTGG TGTTGCAGTA
    AAAGCACATC AGGTGAAAAA TCACATGTGG ATTTTTGTGA ATGCCTTGAT TGAAAACCCA
    ACCTTTGACT CTCAGACGAA AGAAAACATG ACTTTACAAG TCAAGAGCTT TGGATCAACA
    TGCCAATTAA GTGAAAAATT CATCAAAGCT GCAACTGGCT GTGGTATTGT GGAAAGCATT
    CTAAACTGGG TAAAGTTTAA GGCCCAAATC CAATTAAACA AGAAGTGTTC AGCTGTAAAG
    CATAACAGAA TCAAGGGAAT TCCCAAACTT GATGATGCCA ATGACGCAGG GGGTCGAAAC
    TCCAATGAGT GTACACTTAT CCTGACTGAA GGAGACTCAG CTAAAACTTT GGCTGTTTCA
    GGCCTTGGTG TGGTTGGGAG AGACAAATAT GGAGTTTTCC CACTTAGAGG AAAAATACTC
    AATGTTCGAG AAGCTTCTCA TAAACAGATC ATGGAAAATG CTGAAATTAA TAATATCATC
    AAGATCGTGG GTCTTCAGTA CAAGAAAAAC TATGACGATG AAGATTCATT GAAGACTCTT
    CGTTATGGGA AGATAATGAT TATGACAGAT CAGGACCAAG ATGGTTCCCA TATCAAAGGC
    TTGCTGATTA ATTTTATCCA TCATAACTGG CCCTCTCTTC TACGGCATCG TTTTCTGGAG
    GAATTTATCA CTCCGATTGT AAAGGTGTCT AAAAACAAAC AAGAAATGGC ATTCTATAGC
    CTTCCTGAAT TTGAAGAATG GAAGAGTTCT ACTCCAAATC ATAAAAAATG GAAAGTCAAG
    TACTACAAAG GTTTGGGTAC CAGCACATCA AAAGAAGCTA AAGAATACTT TGCAAATATG
    AAAAGACATC GTATTCAGTT CAAATACTCC GGTCCTGAAG ATGATGCTGC CATCAGCTTG
    GCCTTTAGCA AGAAACAGAT AGATGATCGA AAGGAATGGT TAACTCATTT CATGGAAGAT
    AGAAGACAAC GGAAGTTACT TGGCCTTCCT GAGGATTACT TGTATGGGCA AACTACCACA
    TATCTGACAT ACAATGACTT TATAAATAAG GAACTTATCC TGTTCTCAAA TTCTGATAAC
    GAGAGATCTA TCCCATCTAT GGTGGATGGC TTGAAACCAG GTCAAAGAAA GGTTTTGTTT
    ACCTGTTTCA AACGGAATGA CAAGCGAGAG GTGAAGGTTG CCCAGTTGGC TGGGTCAGTG
    GCTGAGATGT CCTCTTATCA TCATGGTGAG ATGTCACTAA TGATGACCAT TATAAATTTG
    GCTCAGAATT TTGTGGGTAG CAATAATCTG AACCTCTTGC AGCCCATTGG TCAGTTTGGT
    ACTAGGCTGC ACGGTGGCAA GGATTCTGCT AGTCCTCGAT ACATCTTCAC AATGCTCAGC
    CCTTTGGCTC GGTTATTGTT TCCACCAAAA GATGATCAGA CTTTAAAGTT TTTATATGAT
    GACAATCAAC GTGTTGAACC TGAATGGTAC ATTCCCATTA TACCCATGGT GCTGATAAAT
    GGTGCTGAAG GAATTGGTAC TGGGTGGTCC TGCAAAATCC CCAACTTTGA TATTCGTGAA
    GTTGTAAATA ATATCAGGCG TTTGATGGAT GGAGAAGAAC CTTTGCCAAT GCTTCCAAGT
    TACAAGAACT TCAGGGGTAC TATTGAAGAG CTGGCTCCAA ATCAGTATGT GATTAGTGGT
    GAAGTGGCTA TACTTAATTC CACAACCATT GAAATCTCAG AGCTTCCTGT CAGAACATGG
    ACCCAGACCT ATAAAGAACA GGTTCTAGAA CCCATGTTGA ATGGCACTGA GAAGACACCA
    CCTCTCATAA CAGATTATAG GGAGTACCAT ACAGACACCA CTGTGAAGTT TGTTGTGAAG
    ATGACTGAAG AGAAACTGGC AGAGGCAGAG AGAGTGGGGC TACACAAAGT CTTCAAACTC
    CAAACTAGTC TCACATGCAA CTCCATGGTG CTTTTTGACC ATGTAGGTTG TTTAAAGAAA
    TATGACACAG TGTTGGATAT TCTAAGAGAC TTCTTTGAAC TAAGGCTTAA ATATTATGGA
    TTAAGAAAAG AATGGCTTCT AGGAATGCTT GGCGCTGAAT CTGCTAAACT GAACAATCAG
    GCTCGCTTTA TCTTAGAGAA AATAGATGGC AAAATAATTA TTGAAAATAA GCCTAAGAAA
    GAATTAATTA AAGTTCTGAT TCAGAGGGGA TATGATTCGG ATCCTGTGAA GGCTTGGAAA
    GAAGCTCAGC AAAAGGTACC AGATGAAGAA GAGAATGAAG AGAGTGACAA TGAAAATGAA
    GCTGAAAAGA GTGACTCTGT AAGAGATTCT GGACCAACCT TCAACTACCT TCTTGATATG
    CCTCTTTGGT ATCTCACCAA GGAGAAGAAG GATGAACTGT GCAAGCTGAG AAATGAAAAA
    GAGCAAGAAC TAGAAACATT AAAGAGAAAG AGTCCAACAG ATTTGTGGAA AGAAGACTTG
    GCTACTTTTG TTGAAGAGCT AGAGGCTGTT GAAACAAAGG AAAAACAAGA AGAACAAATA
    GGACTTCCAG GGAAAGGAGG GAAAGGAAAG GGGAAAAAAA CACAAATGGC TGAAGTTTTG
    CCTTCTCCTT GCGGAAAAAG AGTCATCCCC CGAGTGACCA TAGAGATGAA AGCAGAGGCA
    GAAAAGAAAA ATAAAAAGAA AATTAAGAAT GAAAACACTG AAGGGAGCCC TCAAGAAGAG
    AGTATCGAAA TAGAAGGTCT GAAACAAAGG TTGGAGAAGA AACAGAAAAG AGAACCAGGT
    ACCAAGACAA AGAAGCAAAC TACATTGCCG TTTAAACCCA TCAAAAAAGG AAAGAAGAGA
    AACCCCTGGT CTGATTCAGA ATCAGATATG AGCAGTAATG AAAGTAACTT TGATGTCCCT
    CCACGAGAGG TAGAGCCACG GAGAGCAGCA ACAAAAACCA AATTCACAGT GGATTTGGAT
    TTAGACGACA GTTTCTCAGA TTTTGAAGAA AGAATTCAAG ATGAGGATTC TGTTCCATCA
    GATGTCAGCC CACATAAGAC CAAAGCTTCC CCAAAACGTA CTGCCAAAGA ACTGAAGCCA
    CAGAAAAGTG CCCCCCCAGT AATGGACCTT GATGCTGATG ATGCCAAGGA CAGTGTACCA
    CTTTCTCCAG GTTCTACTGC TGATTTCTTG GCTGAAACTG AAATTGTAAA CCCCCTTCCT
    AAAAAGAAGG TGGACGTGAA GAAGATAGCA CCAAAAAGTC AGTCTTCCAC CTCCACTACA
    GGTGCAAAAA AAAGGGCTGC ACCAAAAGGA ACCAAAAAGG ATCCAGGCTT AAATTCTGAT
    GTCTCTCAAA AGCCTGATCC CCCAAAAACC AAAAATCACC GCAAAAGGAA GCCATCCACT
    TCTGATGATT CCGACTCTAA TTTTGAGAAG ATGATTTCTA AAGCAGCCAC AAACAAGAAA
    TCCAAGGGGG AGAGTGAAGA CTTCCATCTG GACCTAGACT CAGGTGTGGC TCCTCGGGCC
    AAATCTGGAC GTGCAAGGAA ACCTATAAAG TACCTTGAAG AATCAGATGA AGATGATCTG
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005624589.1
    CDS103..4728
    Translation

    Target ORF information:

    RefSeq Version XM_005624532.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005624532.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCTAG TAGTGAAATA TTACCGGCTA CACTGGCTTC TCTGGAGTCC GGTAAATGAA 
    AACATGCAAA TCAGCAAAAC AAAGAAAAAT GAAGATGCTA AGAAAAGACT GTCTATTGAA
    AGGATCTATC AAAAGAAAAC CCAACTGGAA CATATTTTGC TCCGCCCGGA CACCTACATT
    GGCTCTGTGG AATTAGTGAC CCAGCAAATG TGGGTTTACG ATGAAGATAT TGGCATGAAC
    TATAGGGAAG TCACCTTTGT CCCTGGGTTG TACAAAATCT TTGATGAGAT TCTGGTCAAT
    GCTGCAGACA ACAAACAAAG GGACCCAAAA ATGTCTTGTA TTAGAGTCAC AATTGACCCG
    GAAAACAATT TAATTAGTAT ATGGAATAAT GGGAAAGGTA TTCCTGTTGT TGAACACAAA
    GTTGAGAAGA TGTATGTCCC AGCACTCATC TTTGGACAGC TCCTAACTTC TAGTAACTAT
    GATGATGATG AAAAGAAAGT AACAGGTGGT CGAAATGGCT ATGGGGCCAA ATTGTGTAAC
    ATATTCAGTA CCAAGTTTAC TGTGGAAACA GCCAGTAGAG AATACAAGAA AATGTTCAAA
    CAGACGTGGA TGGATAACAT GGGAAGGGCT GGTGAGATGG AACTCAGGCC ATTTAATGGG
    GAAGATTATA CATGTATTAC CTTCCAGCCT GACTTGTCCA AGTTTAAAAT GCAAAGCCTA
    GATAAAGACA TTGTTGCACT GATGGTTAGA AGAGCATACG ATATTGCTGG ATCCACTAAA
    GATGTCAAAG TCTTTCTCAA TGGAAATAAA CTACCAGTAA AAGGATTCCG TAGCTACGTA
    GATATGTATC TGAAGGATAA GGTAGATGAA ACTGGCAATC CATTGAAAAT TATCCATGAA
    CAAGTAAACC CCAGGTGGGA AGTATGTTTA ACAATGAGTG AAAAAGGCTT TCAGCAAATT
    AGCTTCGTCA ACAGCATTGC TACCTCCAAG GGTGGCAGAC ATGTTGATTA TATAGCTGAT
    CAGATTGTGA CTAAACTTGT TGATGTTGTG AAGAAGAAGA ACAAGGGTGG TGTTGCAGTA
    AAAGCACATC AGGTGAAAAA TCACATGTGG ATTTTTGTGA ATGCCTTGAT TGAAAACCCA
    ACCTTTGACT CTCAGACGAA AGAAAACATG ACTTTACAAG TCAAGAGCTT TGGATCAACA
    TGCCAATTAA GTGAAAAATT CATCAAAGCT GCAACTGGCT GTGGTATTGT GGAAAGCATT
    CTAAACTGGG TAAAGTTTAA GGCCCAAATC CAATTAAACA AGAAGTGTTC AGCTGTAAAG
    CATAACAGAA TCAAGGGAAT TCCCAAACTT GATGATGCCA ATGACGCAGG GGGTCGAAAC
    TCCAATGAGT GTACACTTAT CCTGACTGAA GGAGACTCAG CTAAAACTTT GGCTGTTTCA
    GGCCTTGGTG TGGTTGGGAG AGACAAATAT GGAGTTTTCC CACTTAGAGG AAAAATACTC
    AATGTTCGAG AAGCTTCTCA TAAACAGATC ATGGAAAATG CTGAAATTAA TAATATCATC
    AAGATCGTGG GTCTTCAGTA CAAGAAAAAC TATGACGATG AAGATTCATT GAAGACTCTT
    CGTTATGGGA AGATAATGAT TATGACAGAT CAGGACCAAG ATGGTTCCCA TATCAAAGGC
    TTGCTGATTA ATTTTATCCA TCATAACTGG CCCTCTCTTC TACGGCATCG TTTTCTGGAG
    GAATTTATCA CTCCGATTGT AAAGGTGTCT AAAAACAAAC AAGAAATGGC ATTCTATAGC
    CTTCCTGAAT TTGAAGAATG GAAGAGTTCT ACTCCAAATC ATAAAAAATG GAAAGTCAAG
    TACTACAAAG GTTTGGGTAC CAGCACATCA AAAGAAGCTA AAGAATACTT TGCAAATATG
    AAAAGACATC GTATTCAGTT CAAATACTCC GGTCCTGAAG ATGATGCTGC CATCAGCTTG
    GCCTTTAGCA AGAAACAGAT AGATGATCGA AAGGAATGGT TAACTCATTT CATGGAAGAT
    AGAAGACAAC GGAAGTTACT TGGCCTTCCT GAGGATTACT TGTATGGGCA AACTACCACA
    TATCTGACAT ACAATGACTT TATAAATAAG GAACTTATCC TGTTCTCAAA TTCTGATAAC
    GAGAGATCTA TCCCATCTAT GGTGGATGGC TTGAAACCAG GTCAAAGAAA GGTTTTGTTT
    ACCTGTTTCA AACGGAATGA CAAGCGAGAG GTGAAGGTTG CCCAGTTGGC TGGGTCAGTG
    GCTGAGATGT CCTCTTATCA TCATGGTGAG ATGTCACTAA TGATGACCAT TATAAATTTG
    GCTCAGAATT TTGTGGGTAG CAATAATCTG AACCTCTTGC AGCCCATTGG TCAGTTTGGT
    ACTAGGCTGC ACGGTGGCAA GGATTCTGCT AGTCCTCGAT ACATCTTCAC AATGCTCAGC
    CCTTTGGCTC GGTTATTGTT TCCACCAAAA GATGATCAGA CTTTAAAGTT TTTATATGAT
    GACAATCAAC GTGTTGAACC TGAATGGTAC ATTCCCATTA TACCCATGGT GCTGATAAAT
    GGTGCTGAAG GAATTGGTAC TGGGTGGTCC TGCAAAATCC CCAACTTTGA TATTCGTGAA
    GTTGTAAATA ATATCAGGCG TTTGATGGAT GGAGAAGAAC CTTTGCCAAT GCTTCCAAGT
    TACAAGAACT TCAGGGGTAC TATTGAAGAG CTGGCTCCAA ATCAGTATGT GATTAGTGGT
    GAAGTGGCTA TACTTAATTC CACAACCATT GAAATCTCAG AGCTTCCTGT CAGAACATGG
    ACCCAGACCT ATAAAGAACA GGTTCTAGAA CCCATGTTGA ATGGCACTGA GAAGACACCA
    CCTCTCATAA CAGATTATAG GGAGTACCAT ACAGACACCA CTGTGAAGTT TGTTGTGAAG
    ATGACTGAAG AGAAACTGGC AGAGGCAGAG AGAGTGGGGC TACACAAAGT CTTCAAACTC
    CAAACTAGTC TCACATGCAA CTCCATGGTG CTTTTTGACC ATGTAGGTTG TTTAAAGAAA
    TATGACACAG TGTTGGATAT TCTAAGAGAC TTCTTTGAAC TAAGGCTTAA ATATTATGGA
    TTAAGAAAAG AATGGCTTCT AGGAATGCTT GGCGCTGAAT CTGCTAAACT GAACAATCAG
    GCTCGCTTTA TCTTAGAGAA AATAGATGGC AAAATAATTA TTGAAAATAA GCCTAAGAAA
    GAATTAATTA AAGTTCTGAT TCAGAGGGGA TATGATTCGG ATCCTGTGAA GGCTTGGAAA
    GAAGCTCAGC AAAAGGTACC AGATGAAGAA GAGAATGAAG AGAGTGACAA TGAAAATGAA
    GCTGAAAAGA GTGACTCTGT AAGAGATTCT GGACCAACCT TCAACTACCT TCTTGATATG
    CCTCTTTGGT ATCTCACCAA GGAGAAGAAG GATGAACTGT GCAAGCTGAG AAATGAAAAA
    GAGCAAGAAC TAGAAACATT AAAGAGAAAG AGTCCAACAG ATTTGTGGAA AGAAGACTTG
    GCTACTTTTG TTGAAGAGCT AGAGGCTGTT GAAACAAAGG AAAAACAAGA AGAACAAATA
    GGACTTCCAG GGAAAGGAGG GAAAGGAAAG GGGAAAAAAA CACAAATGGC TGAAGTTTTG
    CCTTCTCCTT GCGGAAAAAG AGTCATCCCC CGAGTGACCA TAGAGATGAA AGCAGAGGCA
    GAAAAGAAAA ATAAAAAGAA AATTAAGAAT GAAAACACTG AAGGGAGCCC TCAAGAAGAG
    AGTATCGAAA TAGAAGGTCT GAAACAAAGG TTGGAGAAGA AACAGAAAAG AGAACCAGGT
    ACCAAGACAA AGAAGCAAAC TACATTGCCG TTTAAACCCA TCAAAAAAGG AAAGAAGAGA
    AACCCCTGGT CTGATTCAGA ATCAGATATG AGCAGTAATG AAAGTAACTT TGATGTCCCT
    CCACGAGAGG TAGAGCCACG GAGAGCAGCA ACAAAAACCA AATTCACAGT GGATTTGGAT
    TTAGACGACA GTTTCTCAGA TTTTGAAGAA AGAATTCAAG ATGAGGATTC TGTTCCATCA
    GATGTCAGCC CACATAAGAC CAAAGCTTCC CCAAAACGTA CTGCCAAAGA ACTGAAGCCA
    CAGAAAAGTG CCCCCCCAGT AATGGACCTT GATGCTGATG ATGCCAAGGA CAGTGTACCA
    CTTTCTCCAG GTTCTACTGC TGATTTCTTG GCTGAAACTG AAATTGTAAA CCCCCTTCCT
    AAAAAGAAGG TGGACGTGAA GAAGATAGCA CCAAAAAGTC AGTCTTCCAC CTCCACTACA
    GGTGCAAAAA AAAGGGCTGC ACCAAAAGGA ACCAAAAAGG ATCCAGGCTT AAATTCTGAT
    GTCTCTCAAA AGCCTGATCC CCCAAAAACC AAAAATCACC GCAAAAGGAA GCCATCCACT
    TCTGATGATT CCGACTCTAA TTTTGAGAAG ATGATTTCTA AAGCAGCCAC AAACAAGAAA
    TCCAAGGGGG AGAGTGAAGA CTTCCATCTG GACCTAGACT CAGGTGTGGC TCCTCGGGCC
    AAATCTGGAC GTGCAAGGAA ACCTATAAAG TACCTTGAAG AATCAGATGA AGATGATCTG
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe11751
    Clone ID Related Accession (Same CDS sequence) XM_537646.4 , XM_537646.5
    Accession Version XM_537646.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA topoisomerase 2-alpha isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_537646.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CACCACTGCA GCCGGTAAAT GAAAACATGC AAATCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGGATCT ATCAAAAGAA AACCCAACTG
    GAACATATTT TGCTCCGCCC GGACACCTAC ATTGGCTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TATTGGCATG AACTATAGGG AAGTCACCTT TGTCCCTGGG
    TTGTACAAAA TCTTTGATGA GATTCTGGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGGAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCACTC
    ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGGGC CAAATTGTGT AACATATTCA GTACCAAGTT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACGT GGATGGATAA CATGGGAAGG
    GCTGGTGAGA TGGAACTCAG GCCATTTAAT GGGGAAGATT ATACATGTAT TACCTTCCAG
    CCTGACTTGT CCAAGTTTAA AATGCAAAGC CTAGATAAAG ACATTGTTGC ACTGATGGTT
    AGAAGAGCAT ACGATATTGC TGGATCCACT AAAGATGTCA AAGTCTTTCT CAATGGAAAT
    AAACTACCAG TAAAAGGATT CCGTAGCTAC GTAGATATGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTCG TCAACAGCAT TGCTACCTCC
    AAGGGTGGCA GACATGTTGA TTATATAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TGAATGCCTT GATTGAAAAC CCAACCTTTG ACTCTCAGAC GAAAGAAAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAT TAAGTGAAAA ATTCATCAAA
    GCTGCAACTG GCTGTGGTAT TGTGGAAAGC ATTCTAAACT GGGTAAAGTT TAAGGCCCAA
    ATCCAATTAA ACAAGAAGTG TTCAGCTGTA AAGCATAACA GAATCAAGGG AATTCCCAAA
    CTTGATGATG CCAATGACGC AGGGGGTCGA AACTCCAATG AGTGTACACT TATCCTGACT
    GAAGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGAGTTT TCCCACTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATCATGGAAA ATGCTGAAAT TAATAATATC ATCAAGATCG TGGGTCTTCA GTACAAGAAA
    AACTATGACG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC
    TGGCCCTCTC TTCTACGGCA TCGTTTTCTG GAGGAATTTA TCACTCCGAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAGTACTACA AAGGTTTGGG TACCAGCACA
    TCAAAAGAAG CTAAAGAATA CTTTGCAAAT ATGAAAAGAC ATCGTATTCA GTTCAAATAC
    TCCGGTCCTG AAGATGATGC TGCCATCAGC TTGGCCTTTA GCAAGAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGGAAGTT ACTTGGCCTT
    CCTGAGGATT ACTTGTATGG GCAAACTACC ACATATCTGA CATACAATGA CTTTATAAAT
    AAGGAACTTA TCCTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCATC TATGGTGGAT
    GGCTTGAAAC CAGGTCAAAG AAAGGTTTTG TTTACCTGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT GGCTGGGTCA GTGGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATAAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACTAGGC TGCACGGTGG CAAGGATTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTATT GTTTCCACCA
    AAAGATGATC AGACTTTAAA GTTTTTATAT GATGACAATC AACGTGTTGA ACCTGAATGG
    TACATTCCCA TTATACCCAT GGTGCTGATA AATGGTGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATATTCGT GAAGTTGTAA ATAATATCAG GCGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAGGGG TACTATTGAA
    GAGCTGGCTC CAAATCAGTA TGTGATTAGT GGTGAAGTGG CTATACTTAA TTCCACAACC
    ATTGAAATCT CAGAGCTTCC TGTCAGAACA TGGACCCAGA CCTATAAAGA ACAGGTTCTA
    GAACCCATGT TGAATGGCAC TGAGAAGACA CCACCTCTCA TAACAGATTA TAGGGAGTAC
    CATACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAGAAACT GGCAGAGGCA
    GAGAGAGTGG GGCTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCCATG
    GTGCTTTTTG ACCATGTAGG TTGTTTAAAG AAATATGACA CAGTGTTGGA TATTCTAAGA
    GACTTCTTTG AACTAAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGCGCTG AATCTGCTAA ACTGAACAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TTATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCTC AGCAAAAGGT ACCAGATGAA
    GAAGAGAATG AAGAGAGTGA CAATGAAAAT GAAGCTGAAA AGAGTGACTC TGTAAGAGAT
    TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTCAC CAAGGAGAAG
    AAGGATGAAC TGTGCAAGCT GAGAAATGAA AAAGAGCAAG AACTAGAAAC ATTAAAGAGA
    AAGAGTCCAA CAGATTTGTG GAAAGAAGAC TTGGCTACTT TTGTTGAAGA GCTAGAGGCT
    GTTGAAACAA AGGAAAAACA AGAAGAACAA ATAGGACTTC CAGGGAAAGG AGGGAAAGGA
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CTTGCGGAAA AAGAGTCATC
    CCCCGAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAACA CTGAAGGGAG CCCTCAAGAA GAGAGTATCG AAATAGAAGG TCTGAAACAA
    AGGTTGGAGA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAAGCA AACTACATTG
    CCGTTTAAAC CCATCAAAAA AGGAAAGAAG AGAAACCCCT GGTCTGATTC AGAATCAGAT
    ATGAGCAGTA ATGAAAGTAA CTTTGATGTC CCTCCACGAG AGGTAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AGTGGATTTG GATTTAGACG ACAGTTTCTC AGATTTTGAA
    GAAAGAATTC AAGATGAGGA TTCTGTTCCA TCAGATGTCA GCCCACATAA GACCAAAGCT
    TCCCCAAAAC GTACTGCCAA AGAACTGAAG CCACAGAAAA GTGCCCCCCC AGTAATGGAC
    CTTGATGCTG ATGATGCCAA GGACAGTGTA CCACTTTCTC CAGGTTCTAC TGCTGATTTC
    TTGGCTGAAA CTGAAATTGT AAACCCCCTT CCTAAAAAGA AGGTGGACGT GAAGAAGATA
    GCACCAAAAA GTCAGTCTTC CACCTCCACT ACAGGTGCAA AAAAAAGGGC TGCACCAAAA
    GGAACCAAAA AGGATCCAGG CTTAAATTCT GATGTCTCTC AAAAGCCTGA TCCCCCAAAA
    ACCAAAAATC ACCGCAAAAG GAAGCCATCC ACTTCTGATG ATTCCGACTC TAATTTTGAG
    AAGATGATTT CTAAAGCAGC CACAAACAAG AAATCCAAGG GGGAGAGTGA AGACTTCCAT
    CTGGACCTAG ACTCAGGTGT GGCTCCTCGG GCCAAATCTG GACGTGCAAG GAAACCTATA
    AAGTACCTTG AAGAATCAGA TGAAGATGAT CTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_537646.2
    CDS107..4705
    Translation

    Target ORF information:

    RefSeq Version XM_537646.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_537646.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CACCACTGCA GCCGGTAAAT GAAAACATGC AAATCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGGATCT ATCAAAAGAA AACCCAACTG
    GAACATATTT TGCTCCGCCC GGACACCTAC ATTGGCTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TATTGGCATG AACTATAGGG AAGTCACCTT TGTCCCTGGG
    TTGTACAAAA TCTTTGATGA GATTCTGGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGGAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCACTC
    ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGGGC CAAATTGTGT AACATATTCA GTACCAAGTT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACGT GGATGGATAA CATGGGAAGG
    GCTGGTGAGA TGGAACTCAG GCCATTTAAT GGGGAAGATT ATACATGTAT TACCTTCCAG
    CCTGACTTGT CCAAGTTTAA AATGCAAAGC CTAGATAAAG ACATTGTTGC ACTGATGGTT
    AGAAGAGCAT ACGATATTGC TGGATCCACT AAAGATGTCA AAGTCTTTCT CAATGGAAAT
    AAACTACCAG TAAAAGGATT CCGTAGCTAC GTAGATATGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTCG TCAACAGCAT TGCTACCTCC
    AAGGGTGGCA GACATGTTGA TTATATAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TGAATGCCTT GATTGAAAAC CCAACCTTTG ACTCTCAGAC GAAAGAAAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAT TAAGTGAAAA ATTCATCAAA
    GCTGCAACTG GCTGTGGTAT TGTGGAAAGC ATTCTAAACT GGGTAAAGTT TAAGGCCCAA
    ATCCAATTAA ACAAGAAGTG TTCAGCTGTA AAGCATAACA GAATCAAGGG AATTCCCAAA
    CTTGATGATG CCAATGACGC AGGGGGTCGA AACTCCAATG AGTGTACACT TATCCTGACT
    GAAGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGAGTTT TCCCACTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATCATGGAAA ATGCTGAAAT TAATAATATC ATCAAGATCG TGGGTCTTCA GTACAAGAAA
    AACTATGACG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC
    TGGCCCTCTC TTCTACGGCA TCGTTTTCTG GAGGAATTTA TCACTCCGAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAGTACTACA AAGGTTTGGG TACCAGCACA
    TCAAAAGAAG CTAAAGAATA CTTTGCAAAT ATGAAAAGAC ATCGTATTCA GTTCAAATAC
    TCCGGTCCTG AAGATGATGC TGCCATCAGC TTGGCCTTTA GCAAGAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGGAAGTT ACTTGGCCTT
    CCTGAGGATT ACTTGTATGG GCAAACTACC ACATATCTGA CATACAATGA CTTTATAAAT
    AAGGAACTTA TCCTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCATC TATGGTGGAT
    GGCTTGAAAC CAGGTCAAAG AAAGGTTTTG TTTACCTGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT GGCTGGGTCA GTGGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATAAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACTAGGC TGCACGGTGG CAAGGATTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTATT GTTTCCACCA
    AAAGATGATC AGACTTTAAA GTTTTTATAT GATGACAATC AACGTGTTGA ACCTGAATGG
    TACATTCCCA TTATACCCAT GGTGCTGATA AATGGTGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATATTCGT GAAGTTGTAA ATAATATCAG GCGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAGGGG TACTATTGAA
    GAGCTGGCTC CAAATCAGTA TGTGATTAGT GGTGAAGTGG CTATACTTAA TTCCACAACC
    ATTGAAATCT CAGAGCTTCC TGTCAGAACA TGGACCCAGA CCTATAAAGA ACAGGTTCTA
    GAACCCATGT TGAATGGCAC TGAGAAGACA CCACCTCTCA TAACAGATTA TAGGGAGTAC
    CATACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAGAAACT GGCAGAGGCA
    GAGAGAGTGG GGCTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCCATG
    GTGCTTTTTG ACCATGTAGG TTGTTTAAAG AAATATGACA CAGTGTTGGA TATTCTAAGA
    GACTTCTTTG AACTAAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGCGCTG AATCTGCTAA ACTGAACAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TTATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCTC AGCAAAAGGT ACCAGATGAA
    GAAGAGAATG AAGAGAGTGA CAATGAAAAT GAAGCTGAAA AGAGTGACTC TGTAAGAGAT
    TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTCAC CAAGGAGAAG
    AAGGATGAAC TGTGCAAGCT GAGAAATGAA AAAGAGCAAG AACTAGAAAC ATTAAAGAGA
    AAGAGTCCAA CAGATTTGTG GAAAGAAGAC TTGGCTACTT TTGTTGAAGA GCTAGAGGCT
    GTTGAAACAA AGGAAAAACA AGAAGAACAA ATAGGACTTC CAGGGAAAGG AGGGAAAGGA
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CTTGCGGAAA AAGAGTCATC
    CCCCGAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAACA CTGAAGGGAG CCCTCAAGAA GAGAGTATCG AAATAGAAGG TCTGAAACAA
    AGGTTGGAGA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAAGCA AACTACATTG
    CCGTTTAAAC CCATCAAAAA AGGAAAGAAG AGAAACCCCT GGTCTGATTC AGAATCAGAT
    ATGAGCAGTA ATGAAAGTAA CTTTGATGTC CCTCCACGAG AGGTAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AGTGGATTTG GATTTAGACG ACAGTTTCTC AGATTTTGAA
    GAAAGAATTC AAGATGAGGA TTCTGTTCCA TCAGATGTCA GCCCACATAA GACCAAAGCT
    TCCCCAAAAC GTACTGCCAA AGAACTGAAG CCACAGAAAA GTGCCCCCCC AGTAATGGAC
    CTTGATGCTG ATGATGCCAA GGACAGTGTA CCACTTTCTC CAGGTTCTAC TGCTGATTTC
    TTGGCTGAAA CTGAAATTGT AAACCCCCTT CCTAAAAAGA AGGTGGACGT GAAGAAGATA
    GCACCAAAAA GTCAGTCTTC CACCTCCACT ACAGGTGCAA AAAAAAGGGC TGCACCAAAA
    GGAACCAAAA AGGATCCAGG CTTAAATTCT GATGTCTCTC AAAAGCCTGA TCCCCCAAAA
    ACCAAAAATC ACCGCAAAAG GAAGCCATCC ACTTCTGATG ATTCCGACTC TAATTTTGAG
    AAGATGATTT CTAAAGCAGC CACAAACAAG AAATCCAAGG GGGAGAGTGA AGACTTCCAT
    CTGGACCTAG ACTCAGGTGT GGCTCCTCGG GCCAAATCTG GACGTGCAAG GAAACCTATA
    AAGTACCTTG AAGAATCAGA TGAAGATGAT CTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe11751
    Clone ID Related Accession (Same CDS sequence) XM_537646.4 , XM_537646.5
    Accession Version XM_537646.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA topoisomerase 2-alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537646.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CACCACTGCA GCCGGTAAAT GAAAACATGC AAATCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGGATCT ATCAAAAGAA AACCCAACTG
    GAACATATTT TGCTCCGCCC GGACACCTAC ATTGGCTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TATTGGCATG AACTATAGGG AAGTCACCTT TGTCCCTGGG
    TTGTACAAAA TCTTTGATGA GATTCTGGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGGAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCACTC
    ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGGGC CAAATTGTGT AACATATTCA GTACCAAGTT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACGT GGATGGATAA CATGGGAAGG
    GCTGGTGAGA TGGAACTCAG GCCATTTAAT GGGGAAGATT ATACATGTAT TACCTTCCAG
    CCTGACTTGT CCAAGTTTAA AATGCAAAGC CTAGATAAAG ACATTGTTGC ACTGATGGTT
    AGAAGAGCAT ACGATATTGC TGGATCCACT AAAGATGTCA AAGTCTTTCT CAATGGAAAT
    AAACTACCAG TAAAAGGATT CCGTAGCTAC GTAGATATGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTCG TCAACAGCAT TGCTACCTCC
    AAGGGTGGCA GACATGTTGA TTATATAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TGAATGCCTT GATTGAAAAC CCAACCTTTG ACTCTCAGAC GAAAGAAAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAT TAAGTGAAAA ATTCATCAAA
    GCTGCAACTG GCTGTGGTAT TGTGGAAAGC ATTCTAAACT GGGTAAAGTT TAAGGCCCAA
    ATCCAATTAA ACAAGAAGTG TTCAGCTGTA AAGCATAACA GAATCAAGGG AATTCCCAAA
    CTTGATGATG CCAATGACGC AGGGGGTCGA AACTCCAATG AGTGTACACT TATCCTGACT
    GAAGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGAGTTT TCCCACTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATCATGGAAA ATGCTGAAAT TAATAATATC ATCAAGATCG TGGGTCTTCA GTACAAGAAA
    AACTATGACG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC
    TGGCCCTCTC TTCTACGGCA TCGTTTTCTG GAGGAATTTA TCACTCCGAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAGTACTACA AAGGTTTGGG TACCAGCACA
    TCAAAAGAAG CTAAAGAATA CTTTGCAAAT ATGAAAAGAC ATCGTATTCA GTTCAAATAC
    TCCGGTCCTG AAGATGATGC TGCCATCAGC TTGGCCTTTA GCAAGAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGGAAGTT ACTTGGCCTT
    CCTGAGGATT ACTTGTATGG GCAAACTACC ACATATCTGA CATACAATGA CTTTATAAAT
    AAGGAACTTA TCCTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCATC TATGGTGGAT
    GGCTTGAAAC CAGGTCAAAG AAAGGTTTTG TTTACCTGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT GGCTGGGTCA GTGGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATAAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACTAGGC TGCACGGTGG CAAGGATTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTATT GTTTCCACCA
    AAAGATGATC AGACTTTAAA GTTTTTATAT GATGACAATC AACGTGTTGA ACCTGAATGG
    TACATTCCCA TTATACCCAT GGTGCTGATA AATGGTGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATATTCGT GAAGTTGTAA ATAATATCAG GCGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAGGGG TACTATTGAA
    GAGCTGGCTC CAAATCAGTA TGTGATTAGT GGTGAAGTGG CTATACTTAA TTCCACAACC
    ATTGAAATCT CAGAGCTTCC TGTCAGAACA TGGACCCAGA CCTATAAAGA ACAGGTTCTA
    GAACCCATGT TGAATGGCAC TGAGAAGACA CCACCTCTCA TAACAGATTA TAGGGAGTAC
    CATACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAGAAACT GGCAGAGGCA
    GAGAGAGTGG GGCTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCCATG
    GTGCTTTTTG ACCATGTAGG TTGTTTAAAG AAATATGACA CAGTGTTGGA TATTCTAAGA
    GACTTCTTTG AACTAAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGCGCTG AATCTGCTAA ACTGAACAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TTATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCTC AGCAAAAGGT ACCAGATGAA
    GAAGAGAATG AAGAGAGTGA CAATGAAAAT GAAGCTGAAA AGAGTGACTC TGTAAGAGAT
    TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTCAC CAAGGAGAAG
    AAGGATGAAC TGTGCAAGCT GAGAAATGAA AAAGAGCAAG AACTAGAAAC ATTAAAGAGA
    AAGAGTCCAA CAGATTTGTG GAAAGAAGAC TTGGCTACTT TTGTTGAAGA GCTAGAGGCT
    GTTGAAACAA AGGAAAAACA AGAAGAACAA ATAGGACTTC CAGGGAAAGG AGGGAAAGGA
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CTTGCGGAAA AAGAGTCATC
    CCCCGAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAACA CTGAAGGGAG CCCTCAAGAA GAGAGTATCG AAATAGAAGG TCTGAAACAA
    AGGTTGGAGA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAAGCA AACTACATTG
    CCGTTTAAAC CCATCAAAAA AGGAAAGAAG AGAAACCCCT GGTCTGATTC AGAATCAGAT
    ATGAGCAGTA ATGAAAGTAA CTTTGATGTC CCTCCACGAG AGGTAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AGTGGATTTG GATTTAGACG ACAGTTTCTC AGATTTTGAA
    GAAAGAATTC AAGATGAGGA TTCTGTTCCA TCAGATGTCA GCCCACATAA GACCAAAGCT
    TCCCCAAAAC GTACTGCCAA AGAACTGAAG CCACAGAAAA GTGCCCCCCC AGTAATGGAC
    CTTGATGCTG ATGATGCCAA GGACAGTGTA CCACTTTCTC CAGGTTCTAC TGCTGATTTC
    TTGGCTGAAA CTGAAATTGT AAACCCCCTT CCTAAAAAGA AGGTGGACGT GAAGAAGATA
    GCACCAAAAA GTCAGTCTTC CACCTCCACT ACAGGTGCAA AAAAAAGGGC TGCACCAAAA
    GGAACCAAAA AGGATCCAGG CTTAAATTCT GATGTCTCTC AAAAGCCTGA TCCCCCAAAA
    ACCAAAAATC ACCGCAAAAG GAAGCCATCC ACTTCTGATG ATTCCGACTC TAATTTTGAG
    AAGATGATTT CTAAAGCAGC CACAAACAAG AAATCCAAGG GGGAGAGTGA AGACTTCCAT
    CTGGACCTAG ACTCAGGTGT GGCTCCTCGG GCCAAATCTG GACGTGCAAG GAAACCTATA
    AAGTACCTTG AAGAATCAGA TGAAGATGAT CTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_537646.2
    CDS138..4736
    Translation

    Target ORF information:

    RefSeq Version XM_537646.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA topoisomerase II alpha (TOP2A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_537646.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CACCACTGCA GCCGGTAAAT GAAAACATGC AAATCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGGATCT ATCAAAAGAA AACCCAACTG
    GAACATATTT TGCTCCGCCC GGACACCTAC ATTGGCTCTG TGGAATTAGT GACCCAGCAA
    ATGTGGGTTT ACGATGAAGA TATTGGCATG AACTATAGGG AAGTCACCTT TGTCCCTGGG
    TTGTACAAAA TCTTTGATGA GATTCTGGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGGAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCACTC
    ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGGGC CAAATTGTGT AACATATTCA GTACCAAGTT TACTGTGGAA
    ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACGT GGATGGATAA CATGGGAAGG
    GCTGGTGAGA TGGAACTCAG GCCATTTAAT GGGGAAGATT ATACATGTAT TACCTTCCAG
    CCTGACTTGT CCAAGTTTAA AATGCAAAGC CTAGATAAAG ACATTGTTGC ACTGATGGTT
    AGAAGAGCAT ACGATATTGC TGGATCCACT AAAGATGTCA AAGTCTTTCT CAATGGAAAT
    AAACTACCAG TAAAAGGATT CCGTAGCTAC GTAGATATGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTCG TCAACAGCAT TGCTACCTCC
    AAGGGTGGCA GACATGTTGA TTATATAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG
    TGGATTTTTG TGAATGCCTT GATTGAAAAC CCAACCTTTG ACTCTCAGAC GAAAGAAAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAT TAAGTGAAAA ATTCATCAAA
    GCTGCAACTG GCTGTGGTAT TGTGGAAAGC ATTCTAAACT GGGTAAAGTT TAAGGCCCAA
    ATCCAATTAA ACAAGAAGTG TTCAGCTGTA AAGCATAACA GAATCAAGGG AATTCCCAAA
    CTTGATGATG CCAATGACGC AGGGGGTCGA AACTCCAATG AGTGTACACT TATCCTGACT
    GAAGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA
    TATGGAGTTT TCCCACTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATCATGGAAA ATGCTGAAAT TAATAATATC ATCAAGATCG TGGGTCTTCA GTACAAGAAA
    AACTATGACG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA
    GATCAGGACC AAGATGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC
    TGGCCCTCTC TTCTACGGCA TCGTTTTCTG GAGGAATTTA TCACTCCGAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AATTTGAAGA ATGGAAGAGT
    TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAGTACTACA AAGGTTTGGG TACCAGCACA
    TCAAAAGAAG CTAAAGAATA CTTTGCAAAT ATGAAAAGAC ATCGTATTCA GTTCAAATAC
    TCCGGTCCTG AAGATGATGC TGCCATCAGC TTGGCCTTTA GCAAGAAACA GATAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGGAAGTT ACTTGGCCTT
    CCTGAGGATT ACTTGTATGG GCAAACTACC ACATATCTGA CATACAATGA CTTTATAAAT
    AAGGAACTTA TCCTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCATC TATGGTGGAT
    GGCTTGAAAC CAGGTCAAAG AAAGGTTTTG TTTACCTGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT GGCTGGGTCA GTGGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATAAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT
    CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACTAGGC TGCACGGTGG CAAGGATTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTATT GTTTCCACCA
    AAAGATGATC AGACTTTAAA GTTTTTATAT GATGACAATC AACGTGTTGA ACCTGAATGG
    TACATTCCCA TTATACCCAT GGTGCTGATA AATGGTGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATATTCGT GAAGTTGTAA ATAATATCAG GCGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAGGGG TACTATTGAA
    GAGCTGGCTC CAAATCAGTA TGTGATTAGT GGTGAAGTGG CTATACTTAA TTCCACAACC
    ATTGAAATCT CAGAGCTTCC TGTCAGAACA TGGACCCAGA CCTATAAAGA ACAGGTTCTA
    GAACCCATGT TGAATGGCAC TGAGAAGACA CCACCTCTCA TAACAGATTA TAGGGAGTAC
    CATACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAGAAACT GGCAGAGGCA
    GAGAGAGTGG GGCTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCCATG
    GTGCTTTTTG ACCATGTAGG TTGTTTAAAG AAATATGACA CAGTGTTGGA TATTCTAAGA
    GACTTCTTTG AACTAAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGCGCTG AATCTGCTAA ACTGAACAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TTATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCTC AGCAAAAGGT ACCAGATGAA
    GAAGAGAATG AAGAGAGTGA CAATGAAAAT GAAGCTGAAA AGAGTGACTC TGTAAGAGAT
    TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTCAC CAAGGAGAAG
    AAGGATGAAC TGTGCAAGCT GAGAAATGAA AAAGAGCAAG AACTAGAAAC ATTAAAGAGA
    AAGAGTCCAA CAGATTTGTG GAAAGAAGAC TTGGCTACTT TTGTTGAAGA GCTAGAGGCT
    GTTGAAACAA AGGAAAAACA AGAAGAACAA ATAGGACTTC CAGGGAAAGG AGGGAAAGGA
    AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CTTGCGGAAA AAGAGTCATC
    CCCCGAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG
    AATGAAAACA CTGAAGGGAG CCCTCAAGAA GAGAGTATCG AAATAGAAGG TCTGAAACAA
    AGGTTGGAGA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAAGCA AACTACATTG
    CCGTTTAAAC CCATCAAAAA AGGAAAGAAG AGAAACCCCT GGTCTGATTC AGAATCAGAT
    ATGAGCAGTA ATGAAAGTAA CTTTGATGTC CCTCCACGAG AGGTAGAGCC ACGGAGAGCA
    GCAACAAAAA CCAAATTCAC AGTGGATTTG GATTTAGACG ACAGTTTCTC AGATTTTGAA
    GAAAGAATTC AAGATGAGGA TTCTGTTCCA TCAGATGTCA GCCCACATAA GACCAAAGCT
    TCCCCAAAAC GTACTGCCAA AGAACTGAAG CCACAGAAAA GTGCCCCCCC AGTAATGGAC
    CTTGATGCTG ATGATGCCAA GGACAGTGTA CCACTTTCTC CAGGTTCTAC TGCTGATTTC
    TTGGCTGAAA CTGAAATTGT AAACCCCCTT CCTAAAAAGA AGGTGGACGT GAAGAAGATA
    GCACCAAAAA GTCAGTCTTC CACCTCCACT ACAGGTGCAA AAAAAAGGGC TGCACCAAAA
    GGAACCAAAA AGGATCCAGG CTTAAATTCT GATGTCTCTC AAAAGCCTGA TCCCCCAAAA
    ACCAAAAATC ACCGCAAAAG GAAGCCATCC ACTTCTGATG ATTCCGACTC TAATTTTGAG
    AAGATGATTT CTAAAGCAGC CACAAACAAG AAATCCAAGG GGGAGAGTGA AGACTTCCAT
    CTGGACCTAG ACTCAGGTGT GGCTCCTCGG GCCAAATCTG GACGTGCAAG GAAACCTATA
    AAGTACCTTG AAGAATCAGA TGAAGATGAT CTGTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe11752
    Clone ID Related Accession (Same CDS sequence) XM_005624533.2
    Accession Version XM_005624533.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA topoisomerase 2-alpha isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAAATCA GCAAAACAAA GAAAAATGAA GATGCTAAGA AAAGACTGTC TATTGAAAGG 
    ATCTATCAAA AGAAAACCCA ACTGGAACAT ATTTTGCTCC GCCCGGACAC CTACATTGGC
    TCTGTGGAAT TAGTGACCCA GCAAATGTGG GTTTACGATG AAGATATTGG CATGAACTAT
    AGGGAAGTCA CCTTTGTCCC TGGGTTGTAC AAAATCTTTG ATGAGATTCT GGTCAATGCT
    GCAGACAACA AACAAAGGGA CCCAAAAATG TCTTGTATTA GAGTCACAAT TGACCCGGAA
    AACAATTTAA TTAGTATATG GAATAATGGG AAAGGTATTC CTGTTGTTGA ACACAAAGTT
    GAGAAGATGT ATGTCCCAGC ACTCATCTTT GGACAGCTCC TAACTTCTAG TAACTATGAT
    GATGATGAAA AGAAAGTAAC AGGTGGTCGA AATGGCTATG GGGCCAAATT GTGTAACATA
    TTCAGTACCA AGTTTACTGT GGAAACAGCC AGTAGAGAAT ACAAGAAAAT GTTCAAACAG
    ACGTGGATGG ATAACATGGG AAGGGCTGGT GAGATGGAAC TCAGGCCATT TAATGGGGAA
    GATTATACAT GTATTACCTT CCAGCCTGAC TTGTCCAAGT TTAAAATGCA AAGCCTAGAT
    AAAGACATTG TTGCACTGAT GGTTAGAAGA GCATACGATA TTGCTGGATC CACTAAAGAT
    GTCAAAGTCT TTCTCAATGG AAATAAACTA CCAGTAAAAG GATTCCGTAG CTACGTAGAT
    ATGTATCTGA AGGATAAGGT AGATGAAACT GGCAATCCAT TGAAAATTAT CCATGAACAA
    GTAAACCCCA GGTGGGAAGT ATGTTTAACA ATGAGTGAAA AAGGCTTTCA GCAAATTAGC
    TTCGTCAACA GCATTGCTAC CTCCAAGGGT GGCAGACATG TTGATTATAT AGCTGATCAG
    ATTGTGACTA AACTTGTTGA TGTTGTGAAG AAGAAGAACA AGGGTGGTGT TGCAGTAAAA
    GCACATCAGG TGAAAAATCA CATGTGGATT TTTGTGAATG CCTTGATTGA AAACCCAACC
    TTTGACTCTC AGACGAAAGA AAACATGACT TTACAAGTCA AGAGCTTTGG ATCAACATGC
    CAATTAAGTG AAAAATTCAT CAAAGCTGCA ACTGGCTGTG GTATTGTGGA AAGCATTCTA
    AACTGGGTAA AGTTTAAGGC CCAAATCCAA TTAAACAAGA AGTGTTCAGC TGTAAAGCAT
    AACAGAATCA AGGGAATTCC CAAACTTGAT GATGCCAATG ACGCAGGGGG TCGAAACTCC
    AATGAGTGTA CACTTATCCT GACTGAAGGA GACTCAGCTA AAACTTTGGC TGTTTCAGGC
    CTTGGTGTGG TTGGGAGAGA CAAATATGGA GTTTTCCCAC TTAGAGGAAA AATACTCAAT
    GTTCGAGAAG CTTCTCATAA ACAGATCATG GAAAATGCTG AAATTAATAA TATCATCAAG
    ATCGTGGGTC TTCAGTACAA GAAAAACTAT GACGATGAAG ATTCATTGAA GACTCTTCGT
    TATGGGAAGA TAATGATTAT GACAGATCAG GACCAAGATG GTTCCCATAT CAAAGGCTTG
    CTGATTAATT TTATCCATCA TAACTGGCCC TCTCTTCTAC GGCATCGTTT TCTGGAGGAA
    TTTATCACTC CGATTGTAAA GGTGTCTAAA AACAAACAAG AAATGGCATT CTATAGCCTT
    CCTGAATTTG AAGAATGGAA GAGTTCTACT CCAAATCATA AAAAATGGAA AGTCAAGTAC
    TACAAAGGTT TGGGTACCAG CACATCAAAA GAAGCTAAAG AATACTTTGC AAATATGAAA
    AGACATCGTA TTCAGTTCAA ATACTCCGGT CCTGAAGATG ATGCTGCCAT CAGCTTGGCC
    TTTAGCAAGA AACAGATAGA TGATCGAAAG GAATGGTTAA CTCATTTCAT GGAAGATAGA
    AGACAACGGA AGTTACTTGG CCTTCCTGAG GATTACTTGT ATGGGCAAAC TACCACATAT
    CTGACATACA ATGACTTTAT AAATAAGGAA CTTATCCTGT TCTCAAATTC TGATAACGAG
    AGATCTATCC CATCTATGGT GGATGGCTTG AAACCAGGTC AAAGAAAGGT TTTGTTTACC
    TGTTTCAAAC GGAATGACAA GCGAGAGGTG AAGGTTGCCC AGTTGGCTGG GTCAGTGGCT
    GAGATGTCCT CTTATCATCA TGGTGAGATG TCACTAATGA TGACCATTAT AAATTTGGCT
    CAGAATTTTG TGGGTAGCAA TAATCTGAAC CTCTTGCAGC CCATTGGTCA GTTTGGTACT
    AGGCTGCACG GTGGCAAGGA TTCTGCTAGT CCTCGATACA TCTTCACAAT GCTCAGCCCT
    TTGGCTCGGT TATTGTTTCC ACCAAAAGAT GATCAGACTT TAAAGTTTTT ATATGATGAC
    AATCAACGTG TTGAACCTGA ATGGTACATT CCCATTATAC CCATGGTGCT GATAAATGGT
    GCTGAAGGAA TTGGTACTGG GTGGTCCTGC AAAATCCCCA ACTTTGATAT TCGTGAAGTT
    GTAAATAATA TCAGGCGTTT GATGGATGGA GAAGAACCTT TGCCAATGCT TCCAAGTTAC
    AAGAACTTCA GGGGTACTAT TGAAGAGCTG GCTCCAAATC AGTATGTGAT TAGTGGTGAA
    GTGGCTATAC TTAATTCCAC AACCATTGAA ATCTCAGAGC TTCCTGTCAG AACATGGACC
    CAGACCTATA AAGAACAGGT TCTAGAACCC ATGTTGAATG GCACTGAGAA GACACCACCT
    CTCATAACAG ATTATAGGGA GTACCATACA GACACCACTG TGAAGTTTGT TGTGAAGATG
    ACTGAAGAGA AACTGGCAGA GGCAGAGAGA GTGGGGCTAC ACAAAGTCTT CAAACTCCAA
    ACTAGTCTCA CATGCAACTC CATGGTGCTT TTTGACCATG TAGGTTGTTT AAAGAAATAT
    GACACAGTGT TGGATATTCT AAGAGACTTC TTTGAACTAA GGCTTAAATA TTATGGATTA
    AGAAAAGAAT GGCTTCTAGG AATGCTTGGC GCTGAATCTG CTAAACTGAA CAATCAGGCT
    CGCTTTATCT TAGAGAAAAT AGATGGCAAA ATAATTATTG AAAATAAGCC TAAGAAAGAA
    TTAATTAAAG TTCTGATTCA GAGGGGATAT GATTCGGATC CTGTGAAGGC TTGGAAAGAA
    GCTCAGCAAA AGGTACCAGA TGAAGAAGAG AATGAAGAGA GTGACAATGA AAATGAAGCT
    GAAAAGAGTG ACTCTGTAAG AGATTCTGGA CCAACCTTCA ACTACCTTCT TGATATGCCT
    CTTTGGTATC TCACCAAGGA GAAGAAGGAT GAACTGTGCA AGCTGAGAAA TGAAAAAGAG
    CAAGAACTAG AAACATTAAA GAGAAAGAGT CCAACAGATT TGTGGAAAGA AGACTTGGCT
    ACTTTTGTTG AAGAGCTAGA GGCTGTTGAA ACAAAGGAAA AACAAGAAGA ACAAATAGGA
    CTTCCAGGGA AAGGAGGGAA AGGAAAGGGG AAAAAAACAC AAATGGCTGA AGTTTTGCCT
    TCTCCTTGCG GAAAAAGAGT CATCCCCCGA GTGACCATAG AGATGAAAGC AGAGGCAGAA
    AAGAAAAATA AAAAGAAAAT TAAGAATGAA AACACTGAAG GGAGCCCTCA AGAAGAGAGT
    ATCGAAATAG AAGGTCTGAA ACAAAGGTTG GAGAAGAAAC AGAAAAGAGA ACCAGGTACC
    AAGACAAAGA AGCAAACTAC ATTGCCGTTT AAACCCATCA AAAAAGGAAA GAAGAGAAAC
    CCCTGGTCTG ATTCAGAATC AGATATGAGC AGTAATGAAA GTAACTTTGA TGTCCCTCCA
    CGAGAGGTAG AGCCACGGAG AGCAGCAACA AAAACCAAAT TCACAGTGGA TTTGGATTTA
    GACGACAGTT TCTCAGATTT TGAAGAAAGA ATTCAAGATG AGGATTCTGT TCCATCAGAT
    GTCAGCCCAC ATAAGACCAA AGCTTCCCCA AAACGTACTG CCAAAGAACT GAAGCCACAG
    AAAAGTGCCC CCCCAGTAAT GGACCTTGAT GCTGATGATG CCAAGGACAG TGTACCACTT
    TCTCCAGGTT CTACTGCTGA TTTCTTGGCT GAAACTGAAA TTGTAAACCC CCTTCCTAAA
    AAGAAGGTGG ACGTGAAGAA GATAGCACCA AAAAGTCAGT CTTCCACCTC CACTACAGGT
    GCAAAAAAAA GGGCTGCACC AAAAGGAACC AAAAAGGATC CAGGCTTAAA TTCTGATGTC
    TCTCAAAAGC CTGATCCCCC AAAAACCAAA AATCACCGCA AAAGGAAGCC ATCCACTTCT
    GATGATTCCG ACTCTAATTT TGAGAAGATG ATTTCTAAAG CAGCCACAAA CAAGAAATCC
    AAGGGGGAGA GTGAAGACTT CCATCTGGAC CTAGACTCAG GTGTGGCTCC TCGGGCCAAA
    TCTGGACGTG CAAGGAAACC TATAAAGTAC CTTGAAGAAT CAGATGAAGA TGATCTGTTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005624590.1
    CDS151..4713
    Translation

    Target ORF information:

    RefSeq Version XM_005624533.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005624533.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAAATCA GCAAAACAAA GAAAAATGAA GATGCTAAGA AAAGACTGTC TATTGAAAGG 
    ATCTATCAAA AGAAAACCCA ACTGGAACAT ATTTTGCTCC GCCCGGACAC CTACATTGGC
    TCTGTGGAAT TAGTGACCCA GCAAATGTGG GTTTACGATG AAGATATTGG CATGAACTAT
    AGGGAAGTCA CCTTTGTCCC TGGGTTGTAC AAAATCTTTG ATGAGATTCT GGTCAATGCT
    GCAGACAACA AACAAAGGGA CCCAAAAATG TCTTGTATTA GAGTCACAAT TGACCCGGAA
    AACAATTTAA TTAGTATATG GAATAATGGG AAAGGTATTC CTGTTGTTGA ACACAAAGTT
    GAGAAGATGT ATGTCCCAGC ACTCATCTTT GGACAGCTCC TAACTTCTAG TAACTATGAT
    GATGATGAAA AGAAAGTAAC AGGTGGTCGA AATGGCTATG GGGCCAAATT GTGTAACATA
    TTCAGTACCA AGTTTACTGT GGAAACAGCC AGTAGAGAAT ACAAGAAAAT GTTCAAACAG
    ACGTGGATGG ATAACATGGG AAGGGCTGGT GAGATGGAAC TCAGGCCATT TAATGGGGAA
    GATTATACAT GTATTACCTT CCAGCCTGAC TTGTCCAAGT TTAAAATGCA AAGCCTAGAT
    AAAGACATTG TTGCACTGAT GGTTAGAAGA GCATACGATA TTGCTGGATC CACTAAAGAT
    GTCAAAGTCT TTCTCAATGG AAATAAACTA CCAGTAAAAG GATTCCGTAG CTACGTAGAT
    ATGTATCTGA AGGATAAGGT AGATGAAACT GGCAATCCAT TGAAAATTAT CCATGAACAA
    GTAAACCCCA GGTGGGAAGT ATGTTTAACA ATGAGTGAAA AAGGCTTTCA GCAAATTAGC
    TTCGTCAACA GCATTGCTAC CTCCAAGGGT GGCAGACATG TTGATTATAT AGCTGATCAG
    ATTGTGACTA AACTTGTTGA TGTTGTGAAG AAGAAGAACA AGGGTGGTGT TGCAGTAAAA
    GCACATCAGG TGAAAAATCA CATGTGGATT TTTGTGAATG CCTTGATTGA AAACCCAACC
    TTTGACTCTC AGACGAAAGA AAACATGACT TTACAAGTCA AGAGCTTTGG ATCAACATGC
    CAATTAAGTG AAAAATTCAT CAAAGCTGCA ACTGGCTGTG GTATTGTGGA AAGCATTCTA
    AACTGGGTAA AGTTTAAGGC CCAAATCCAA TTAAACAAGA AGTGTTCAGC TGTAAAGCAT
    AACAGAATCA AGGGAATTCC CAAACTTGAT GATGCCAATG ACGCAGGGGG TCGAAACTCC
    AATGAGTGTA CACTTATCCT GACTGAAGGA GACTCAGCTA AAACTTTGGC TGTTTCAGGC
    CTTGGTGTGG TTGGGAGAGA CAAATATGGA GTTTTCCCAC TTAGAGGAAA AATACTCAAT
    GTTCGAGAAG CTTCTCATAA ACAGATCATG GAAAATGCTG AAATTAATAA TATCATCAAG
    ATCGTGGGTC TTCAGTACAA GAAAAACTAT GACGATGAAG ATTCATTGAA GACTCTTCGT
    TATGGGAAGA TAATGATTAT GACAGATCAG GACCAAGATG GTTCCCATAT CAAAGGCTTG
    CTGATTAATT TTATCCATCA TAACTGGCCC TCTCTTCTAC GGCATCGTTT TCTGGAGGAA
    TTTATCACTC CGATTGTAAA GGTGTCTAAA AACAAACAAG AAATGGCATT CTATAGCCTT
    CCTGAATTTG AAGAATGGAA GAGTTCTACT CCAAATCATA AAAAATGGAA AGTCAAGTAC
    TACAAAGGTT TGGGTACCAG CACATCAAAA GAAGCTAAAG AATACTTTGC AAATATGAAA
    AGACATCGTA TTCAGTTCAA ATACTCCGGT CCTGAAGATG ATGCTGCCAT CAGCTTGGCC
    TTTAGCAAGA AACAGATAGA TGATCGAAAG GAATGGTTAA CTCATTTCAT GGAAGATAGA
    AGACAACGGA AGTTACTTGG CCTTCCTGAG GATTACTTGT ATGGGCAAAC TACCACATAT
    CTGACATACA ATGACTTTAT AAATAAGGAA CTTATCCTGT TCTCAAATTC TGATAACGAG
    AGATCTATCC CATCTATGGT GGATGGCTTG AAACCAGGTC AAAGAAAGGT TTTGTTTACC
    TGTTTCAAAC GGAATGACAA GCGAGAGGTG AAGGTTGCCC AGTTGGCTGG GTCAGTGGCT
    GAGATGTCCT CTTATCATCA TGGTGAGATG TCACTAATGA TGACCATTAT AAATTTGGCT
    CAGAATTTTG TGGGTAGCAA TAATCTGAAC CTCTTGCAGC CCATTGGTCA GTTTGGTACT
    AGGCTGCACG GTGGCAAGGA TTCTGCTAGT CCTCGATACA TCTTCACAAT GCTCAGCCCT
    TTGGCTCGGT TATTGTTTCC ACCAAAAGAT GATCAGACTT TAAAGTTTTT ATATGATGAC
    AATCAACGTG TTGAACCTGA ATGGTACATT CCCATTATAC CCATGGTGCT GATAAATGGT
    GCTGAAGGAA TTGGTACTGG GTGGTCCTGC AAAATCCCCA ACTTTGATAT TCGTGAAGTT
    GTAAATAATA TCAGGCGTTT GATGGATGGA GAAGAACCTT TGCCAATGCT TCCAAGTTAC
    AAGAACTTCA GGGGTACTAT TGAAGAGCTG GCTCCAAATC AGTATGTGAT TAGTGGTGAA
    GTGGCTATAC TTAATTCCAC AACCATTGAA ATCTCAGAGC TTCCTGTCAG AACATGGACC
    CAGACCTATA AAGAACAGGT TCTAGAACCC ATGTTGAATG GCACTGAGAA GACACCACCT
    CTCATAACAG ATTATAGGGA GTACCATACA GACACCACTG TGAAGTTTGT TGTGAAGATG
    ACTGAAGAGA AACTGGCAGA GGCAGAGAGA GTGGGGCTAC ACAAAGTCTT CAAACTCCAA
    ACTAGTCTCA CATGCAACTC CATGGTGCTT TTTGACCATG TAGGTTGTTT AAAGAAATAT
    GACACAGTGT TGGATATTCT AAGAGACTTC TTTGAACTAA GGCTTAAATA TTATGGATTA
    AGAAAAGAAT GGCTTCTAGG AATGCTTGGC GCTGAATCTG CTAAACTGAA CAATCAGGCT
    CGCTTTATCT TAGAGAAAAT AGATGGCAAA ATAATTATTG AAAATAAGCC TAAGAAAGAA
    TTAATTAAAG TTCTGATTCA GAGGGGATAT GATTCGGATC CTGTGAAGGC TTGGAAAGAA
    GCTCAGCAAA AGGTACCAGA TGAAGAAGAG AATGAAGAGA GTGACAATGA AAATGAAGCT
    GAAAAGAGTG ACTCTGTAAG AGATTCTGGA CCAACCTTCA ACTACCTTCT TGATATGCCT
    CTTTGGTATC TCACCAAGGA GAAGAAGGAT GAACTGTGCA AGCTGAGAAA TGAAAAAGAG
    CAAGAACTAG AAACATTAAA GAGAAAGAGT CCAACAGATT TGTGGAAAGA AGACTTGGCT
    ACTTTTGTTG AAGAGCTAGA GGCTGTTGAA ACAAAGGAAA AACAAGAAGA ACAAATAGGA
    CTTCCAGGGA AAGGAGGGAA AGGAAAGGGG AAAAAAACAC AAATGGCTGA AGTTTTGCCT
    TCTCCTTGCG GAAAAAGAGT CATCCCCCGA GTGACCATAG AGATGAAAGC AGAGGCAGAA
    AAGAAAAATA AAAAGAAAAT TAAGAATGAA AACACTGAAG GGAGCCCTCA AGAAGAGAGT
    ATCGAAATAG AAGGTCTGAA ACAAAGGTTG GAGAAGAAAC AGAAAAGAGA ACCAGGTACC
    AAGACAAAGA AGCAAACTAC ATTGCCGTTT AAACCCATCA AAAAAGGAAA GAAGAGAAAC
    CCCTGGTCTG ATTCAGAATC AGATATGAGC AGTAATGAAA GTAACTTTGA TGTCCCTCCA
    CGAGAGGTAG AGCCACGGAG AGCAGCAACA AAAACCAAAT TCACAGTGGA TTTGGATTTA
    GACGACAGTT TCTCAGATTT TGAAGAAAGA ATTCAAGATG AGGATTCTGT TCCATCAGAT
    GTCAGCCCAC ATAAGACCAA AGCTTCCCCA AAACGTACTG CCAAAGAACT GAAGCCACAG
    AAAAGTGCCC CCCCAGTAAT GGACCTTGAT GCTGATGATG CCAAGGACAG TGTACCACTT
    TCTCCAGGTT CTACTGCTGA TTTCTTGGCT GAAACTGAAA TTGTAAACCC CCTTCCTAAA
    AAGAAGGTGG ACGTGAAGAA GATAGCACCA AAAAGTCAGT CTTCCACCTC CACTACAGGT
    GCAAAAAAAA GGGCTGCACC AAAAGGAACC AAAAAGGATC CAGGCTTAAA TTCTGATGTC
    TCTCAAAAGC CTGATCCCCC AAAAACCAAA AATCACCGCA AAAGGAAGCC ATCCACTTCT
    GATGATTCCG ACTCTAATTT TGAGAAGATG ATTTCTAAAG CAGCCACAAA CAAGAAATCC
    AAGGGGGAGA GTGAAGACTT CCATCTGGAC CTAGACTCAG GTGTGGCTCC TCGGGCCAAA
    TCTGGACGTG CAAGGAAACC TATAAAGTAC CTTGAAGAAT CAGATGAAGA TGATCTGTTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.