LRRC8B cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRRC8B
Entrez Gene ID	479958
Full Name	leucine rich repeat containing 8 VRAC subunit B
General protein information	Preferred Names leucine rich repeat containing 8 VRAC subunit B Names volume-regulated anion channel subunit LRRC8B leucine rich repeat containing 8 family member B
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022420527.1	XP_022276235.1	volume-regulated anion channel subunit LRRC8B isoform X1
XM_005621953.3	XP_005622010.1	volume-regulated anion channel subunit LRRC8B isoform X1
XM_022420528.1	XP_022276236.1	volume-regulated anion channel subunit LRRC8B isoform X2
XM_005621953.2	XP_005622010.1	volume-regulated anion channel subunit LRRC8B

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8B	NP_056165.1
Rattus norvegicus (Norway rat)	Lrrc8b	NP_001100674.1
Gallus gallus (chicken)	LRRC8B	XP_004936694.1
	LRRC8B	XP_002801670.1
Pan troglodytes (chimpanzee)	LRRC8B	XP_001150409.1
Bos taurus (cattle)	LRRC8B	XP_005204398.1
Mus musculus (house mouse)	Lrrc8b	NP_001028722.1
Canis lupus familiaris (dog)	LRRC8B	XP_005622010.1
Xenopus tropicalis (tropical clawed frog)	lrrc8b	XP_002941172.1

Gene Ontology

The following LRRC8B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRC8B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe08621	XM_005621953.3 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit B (LRRC8B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe08621	XM_022420527.1 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit B (LRRC8B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe08623	XM_022420528.1 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit B (LRRC8B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OCe08621	XM_005621953.2	Canis lupus familiaris leucine rich repeat containing 8 family, member B (LRRC8B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRRC8B ( XM_022420527.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022276235.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit B (LRRC8B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe08621
Clone ID Related Accession (Same CDS sequence)	XM_022420527.1 , XM_005621953.3 , XM_005621953.2
Accession Version	XM_022420527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACAC TAACAGAGCT GAAATGTTTA GCAGACGCCC AGTCATCTTA TCACATCCTA 
AAACCATGGT GGGACGTCTT CTGGTATTAC ATTACCCTGA TCATGCTGCT GGTGGCTGTG 
CTGGCCGGAG CTCTCCAGCT CACACAGAGC AGGGTTCTGT GCTGTCTTCC ATGTAAGGTG 
GAATATGACA ATCACTGTGC CGTGCCTTGG GACATCCTGA GAGCCAGCGC AAACATGTCC 
TCTGATCCCG GGACACCGCT TCCGCTCCCC CTCAGAATCC AGAACGACCT CCACCGACAG 
CAGTACTCCT ACATTGACGC CGTCTGTTAT GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTACC TGGTGCTCTT GCACACGCTC ATCTTTGCAG CCTGTAGCAA CTTCTGGCTA 
CACTATCCCA GTACCAGTTC TCGGCTCGAG CATTTTGTGG CCATCCTTCA CAAGTGCTTC 
GATTCCCCGT GGACCACCCG GGCCCTGTCC GAAACGGTGG CCGAGCAATC AGTGAGGCCC 
CTGACATTCT CCAAGTCCAA GGTTCTGCTG TCGTCCTCAG GGTGCTCAGC CGACGTCGAT 
TCCAACAAGC AGTCATTGCC CTACCCACAG CCTGGCTTGG AGTCAGCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGTGAACAGG CCAAAGCCAT CTTTGAAAAA 
GTGAAAAGGT TCCGCCTGCA CGTGGAGCAG AAGGACATCA TTTATAGAGT GTATCTGAAG 
CAGATCATAG TGAAAGTCAT TTTGTTCGTC CTCATCATAA CTTATGTTCC GTATTTTCTA 
ACCTACATCA GTCTTGAAAT CGACTGTTCG GTTGATGTGC AAGCTTTCAC AGGCTACAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATATTTAAGG TCCTGGCTTC CTTTTATGTC 
ATTTTGGTTA TACTCTATGG TCTCACCTCC TCCTACAGCT TATGGTGGAT GCTGAGGAGT 
TCTCTGAAGC AATATTCCTT TGAGGCGTTG AGAGAGAAAA GCAACTATAG CGATATCCCC 
GACGTGAAGA ACGACTTCGC CTTCATCCTC CATCTGGCAG ATCAGTACGA TCCTCTTTAT 
TCCAAACGCT TCTCCATATT CCTGTCGGAG GTCAGCGAGA ACAAGCTGAA ACAGATCAAC 
CTCAACAACG AATGGACAGT TGAGAAGCTG AAGAGTAAGC TTGTGAAAAA CTCACAGGAC 
AAGATAGAGC TGCATCTTTT TATGCTAAAC GGTCTTCCGG ACAACGTCTT TGAGCTGACA 
GAAATCGAAG TGCTGAGCCT GGAGCTGATC CCTGAGGTCA AGCTGCCCTC TGCGGTCTCG 
CAGCTGGTCA ACCTCAGGGA GCTTCACGTG TACCACTCGT CCCTGGTGGT GGACCATCCA 
GCGCTGGGCT TCCTGGAGGA GAACCTAAAA ATCCTTCGCC TGAAATTCAC CGAAATGGGG 
AAGATCCCAC GCTGGGTATT TCACCTGAAG AATCTCAAGG AACTTTATCT GTCAGGCTGT 
GTTCTTCCGG AACAGCTGAG TACCATGCAG CTGGAGGGCT TCCAGGACTT GAAAAACCTG 
CGGACCCTCT ACCTGAAGAG CAGCCTCTCC CGGATCCCAC AGGTCATTAC AGACCTCCTG 
CCTTCTCTGC AGAAGCTGGC CCTCGATAAC GAGGGAAGCA AGCTGGTTGT GTTGAACAAC 
TTGAAAAAGA TGGTCAATCT GAAGAGCCTC GAGCTGATCA GCTGTGACCT GGAACGCATC 
CCACACTCTA TTTTCAGCCT CAACAATTTG CATGAGTTAG ACCTAAAAGA GAATAACCTT 
AAAACTGTGG AAGAGATCAT TAGCTTTCAG CATCTCCAGA ATCTTTCCTG CTTAAAGCTG 
TGGCATAATA ACATTGCTTA TATTCCTGCC CAGATTGGGG CACTATCTAA TCTAGAGCAG 
CTCTCTTTGA ACCATAATAA TATTGAGAAT CTGCCCCTGC AGCTTTTCCT ATGCACCAAA 
CTACATTATT TGGATCTAAG CTATAACCAC CTGACCTTCA TTCCAGAAGA AATCCAGTAT 
CTGAGTAATT TGCAGTACTT TGCTGTGACC AACAACAATA TTGAGATGCT ACCAGATGGG 
CTGTTTCAGT GCAAAAAGCT GCAGTGTTTA CTTTTGGGGA AAAATAGCCT GATGAGTTTA 
TCCCCTCACG TGGGTGAGCT GTCGAACCTT ACTCATCTGG AGCTCATTGG TAATTACCTG 
GAAACACTTC CTCCTGAACT GGAAGGCTGT CCGTCCCTAA AGCGGAGCTG TCTGATTGTA 
GAGGAGAACT TGCTCAACAC TCTTCCTCTC CCTGTGACAG AACGTTTGCA GACGTGCTTA 
GATAAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276235.1
CDS	388..2799
Translation	MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEYDNHCAVPWDILRASANMSSDPGTPLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVAILHKCFDSPWTTRALSETVAEQSVRPLTFSKSKVLLSSSGCSADVDSNKQSLPYPQPGLESAGIESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQKDIIYRVYLKQIIVKVILFVLIITYVPYFLTYISLEIDCSVDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVILYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVEKLKSKLVKNSQDKIELHLFMLNGLPDNVFELTEIEVLSLELIPEVKLPSAVSQLVNLRELHVYHSSLVVDHPALGFLEENLKILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSTMQLEGFQDLKNLRTLYLKSSLSRIPQVITDLLPSLQKLALDNEGSKLVVLNNLKKMVNLKSLELISCDLERIPHSIFSLNNLHELDLKENNLKTVEEIISFQHLQNLSCLKLWHNNIAYIPAQIGALSNLEQLSLNHNNIENLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLSNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGKNSLMSLSPHVGELSNLTHLELIGNYLETLPPELEGCPSLKRSCLIVEENLLNTLPLPVTERLQTCLDKC

Target ORF information:

RefSeq Version	XM_022420527.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit B (LRRC8B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420527.1

ORF Insert Sequence:

ATGATTACAC TAACAGAGCT GAAATGTTTA GCAGACGCCC AGTCATCTTA TCACATCCTA 
AAACCATGGT GGGACGTCTT CTGGTATTAC ATTACCCTGA TCATGCTGCT GGTGGCTGTG 
CTGGCCGGAG CTCTCCAGCT CACACAGAGC AGGGTTCTGT GCTGTCTTCC ATGTAAGGTG 
GAATATGACA ATCACTGTGC CGTGCCTTGG GACATCCTGA GAGCCAGCGC AAACATGTCC 
TCTGATCCCG GGACACCGCT TCCGCTCCCC CTCAGAATCC AGAACGACCT CCACCGACAG 
CAGTACTCCT ACATTGACGC CGTCTGTTAT GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTACC TGGTGCTCTT GCACACGCTC ATCTTTGCAG CCTGTAGCAA CTTCTGGCTA 
CACTATCCCA GTACCAGTTC TCGGCTCGAG CATTTTGTGG CCATCCTTCA CAAGTGCTTC 
GATTCCCCGT GGACCACCCG GGCCCTGTCC GAAACGGTGG CCGAGCAATC AGTGAGGCCC 
CTGACATTCT CCAAGTCCAA GGTTCTGCTG TCGTCCTCAG GGTGCTCAGC CGACGTCGAT 
TCCAACAAGC AGTCATTGCC CTACCCACAG CCTGGCTTGG AGTCAGCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGTGAACAGG CCAAAGCCAT CTTTGAAAAA 
GTGAAAAGGT TCCGCCTGCA CGTGGAGCAG AAGGACATCA TTTATAGAGT GTATCTGAAG 
CAGATCATAG TGAAAGTCAT TTTGTTCGTC CTCATCATAA CTTATGTTCC GTATTTTCTA 
ACCTACATCA GTCTTGAAAT CGACTGTTCG GTTGATGTGC AAGCTTTCAC AGGCTACAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATATTTAAGG TCCTGGCTTC CTTTTATGTC 
ATTTTGGTTA TACTCTATGG TCTCACCTCC TCCTACAGCT TATGGTGGAT GCTGAGGAGT 
TCTCTGAAGC AATATTCCTT TGAGGCGTTG AGAGAGAAAA GCAACTATAG CGATATCCCC 
GACGTGAAGA ACGACTTCGC CTTCATCCTC CATCTGGCAG ATCAGTACGA TCCTCTTTAT 
TCCAAACGCT TCTCCATATT CCTGTCGGAG GTCAGCGAGA ACAAGCTGAA ACAGATCAAC 
CTCAACAACG AATGGACAGT TGAGAAGCTG AAGAGTAAGC TTGTGAAAAA CTCACAGGAC 
AAGATAGAGC TGCATCTTTT TATGCTAAAC GGTCTTCCGG ACAACGTCTT TGAGCTGACA 
GAAATCGAAG TGCTGAGCCT GGAGCTGATC CCTGAGGTCA AGCTGCCCTC TGCGGTCTCG 
CAGCTGGTCA ACCTCAGGGA GCTTCACGTG TACCACTCGT CCCTGGTGGT GGACCATCCA 
GCGCTGGGCT TCCTGGAGGA GAACCTAAAA ATCCTTCGCC TGAAATTCAC CGAAATGGGG 
AAGATCCCAC GCTGGGTATT TCACCTGAAG AATCTCAAGG AACTTTATCT GTCAGGCTGT 
GTTCTTCCGG AACAGCTGAG TACCATGCAG CTGGAGGGCT TCCAGGACTT GAAAAACCTG 
CGGACCCTCT ACCTGAAGAG CAGCCTCTCC CGGATCCCAC AGGTCATTAC AGACCTCCTG 
CCTTCTCTGC AGAAGCTGGC CCTCGATAAC GAGGGAAGCA AGCTGGTTGT GTTGAACAAC 
TTGAAAAAGA TGGTCAATCT GAAGAGCCTC GAGCTGATCA GCTGTGACCT GGAACGCATC 
CCACACTCTA TTTTCAGCCT CAACAATTTG CATGAGTTAG ACCTAAAAGA GAATAACCTT 
AAAACTGTGG AAGAGATCAT TAGCTTTCAG CATCTCCAGA ATCTTTCCTG CTTAAAGCTG 
TGGCATAATA ACATTGCTTA TATTCCTGCC CAGATTGGGG CACTATCTAA TCTAGAGCAG 
CTCTCTTTGA ACCATAATAA TATTGAGAAT CTGCCCCTGC AGCTTTTCCT ATGCACCAAA 
CTACATTATT TGGATCTAAG CTATAACCAC CTGACCTTCA TTCCAGAAGA AATCCAGTAT 
CTGAGTAATT TGCAGTACTT TGCTGTGACC AACAACAATA TTGAGATGCT ACCAGATGGG 
CTGTTTCAGT GCAAAAAGCT GCAGTGTTTA CTTTTGGGGA AAAATAGCCT GATGAGTTTA 
TCCCCTCACG TGGGTGAGCT GTCGAACCTT ACTCATCTGG AGCTCATTGG TAATTACCTG 
GAAACACTTC CTCCTGAACT GGAAGGCTGT CCGTCCCTAA AGCGGAGCTG TCTGATTGTA 
GAGGAGAACT TGCTCAACAC TCTTCCTCTC CCTGTGACAG AACGTTTGCA GACGTGCTTA 
GATAAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8B ( XM_005621953.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005622010.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit B (LRRC8B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe08621
Clone ID Related Accession (Same CDS sequence)	XM_022420527.1 , XM_005621953.3 , XM_005621953.2
Accession Version	XM_005621953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621953.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACAC TAACAGAGCT GAAATGTTTA GCAGACGCCC AGTCATCTTA TCACATCCTA 
AAACCATGGT GGGACGTCTT CTGGTATTAC ATTACCCTGA TCATGCTGCT GGTGGCTGTG 
CTGGCCGGAG CTCTCCAGCT CACACAGAGC AGGGTTCTGT GCTGTCTTCC ATGTAAGGTG 
GAATATGACA ATCACTGTGC CGTGCCTTGG GACATCCTGA GAGCCAGCGC AAACATGTCC 
TCTGATCCCG GGACACCGCT TCCGCTCCCC CTCAGAATCC AGAACGACCT CCACCGACAG 
CAGTACTCCT ACATTGACGC CGTCTGTTAT GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTACC TGGTGCTCTT GCACACGCTC ATCTTTGCAG CCTGTAGCAA CTTCTGGCTA 
CACTATCCCA GTACCAGTTC TCGGCTCGAG CATTTTGTGG CCATCCTTCA CAAGTGCTTC 
GATTCCCCGT GGACCACCCG GGCCCTGTCC GAAACGGTGG CCGAGCAATC AGTGAGGCCC 
CTGACATTCT CCAAGTCCAA GGTTCTGCTG TCGTCCTCAG GGTGCTCAGC CGACGTCGAT 
TCCAACAAGC AGTCATTGCC CTACCCACAG CCTGGCTTGG AGTCAGCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGTGAACAGG CCAAAGCCAT CTTTGAAAAA 
GTGAAAAGGT TCCGCCTGCA CGTGGAGCAG AAGGACATCA TTTATAGAGT GTATCTGAAG 
CAGATCATAG TGAAAGTCAT TTTGTTCGTC CTCATCATAA CTTATGTTCC GTATTTTCTA 
ACCTACATCA GTCTTGAAAT CGACTGTTCG GTTGATGTGC AAGCTTTCAC AGGCTACAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATATTTAAGG TCCTGGCTTC CTTTTATGTC 
ATTTTGGTTA TACTCTATGG TCTCACCTCC TCCTACAGCT TATGGTGGAT GCTGAGGAGT 
TCTCTGAAGC AATATTCCTT TGAGGCGTTG AGAGAGAAAA GCAACTATAG CGATATCCCC 
GACGTGAAGA ACGACTTCGC CTTCATCCTC CATCTGGCAG ATCAGTACGA TCCTCTTTAT 
TCCAAACGCT TCTCCATATT CCTGTCGGAG GTCAGCGAGA ACAAGCTGAA ACAGATCAAC 
CTCAACAACG AATGGACAGT TGAGAAGCTG AAGAGTAAGC TTGTGAAAAA CTCACAGGAC 
AAGATAGAGC TGCATCTTTT TATGCTAAAC GGTCTTCCGG ACAACGTCTT TGAGCTGACA 
GAAATCGAAG TGCTGAGCCT GGAGCTGATC CCTGAGGTCA AGCTGCCCTC TGCGGTCTCG 
CAGCTGGTCA ACCTCAGGGA GCTTCACGTG TACCACTCGT CCCTGGTGGT GGACCATCCA 
GCGCTGGGCT TCCTGGAGGA GAACCTAAAA ATCCTTCGCC TGAAATTCAC CGAAATGGGG 
AAGATCCCAC GCTGGGTATT TCACCTGAAG AATCTCAAGG AACTTTATCT GTCAGGCTGT 
GTTCTTCCGG AACAGCTGAG TACCATGCAG CTGGAGGGCT TCCAGGACTT GAAAAACCTG 
CGGACCCTCT ACCTGAAGAG CAGCCTCTCC CGGATCCCAC AGGTCATTAC AGACCTCCTG 
CCTTCTCTGC AGAAGCTGGC CCTCGATAAC GAGGGAAGCA AGCTGGTTGT GTTGAACAAC 
TTGAAAAAGA TGGTCAATCT GAAGAGCCTC GAGCTGATCA GCTGTGACCT GGAACGCATC 
CCACACTCTA TTTTCAGCCT CAACAATTTG CATGAGTTAG ACCTAAAAGA GAATAACCTT 
AAAACTGTGG AAGAGATCAT TAGCTTTCAG CATCTCCAGA ATCTTTCCTG CTTAAAGCTG 
TGGCATAATA ACATTGCTTA TATTCCTGCC CAGATTGGGG CACTATCTAA TCTAGAGCAG 
CTCTCTTTGA ACCATAATAA TATTGAGAAT CTGCCCCTGC AGCTTTTCCT ATGCACCAAA 
CTACATTATT TGGATCTAAG CTATAACCAC CTGACCTTCA TTCCAGAAGA AATCCAGTAT 
CTGAGTAATT TGCAGTACTT TGCTGTGACC AACAACAATA TTGAGATGCT ACCAGATGGG 
CTGTTTCAGT GCAAAAAGCT GCAGTGTTTA CTTTTGGGGA AAAATAGCCT GATGAGTTTA 
TCCCCTCACG TGGGTGAGCT GTCGAACCTT ACTCATCTGG AGCTCATTGG TAATTACCTG 
GAAACACTTC CTCCTGAACT GGAAGGCTGT CCGTCCCTAA AGCGGAGCTG TCTGATTGTA 
GAGGAGAACT TGCTCAACAC TCTTCCTCTC CCTGTGACAG AACGTTTGCA GACGTGCTTA 
GATAAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622010.1
CDS	244..2655
Translation	MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEYDNHCAVPWDILRASANMSSDPGTPLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVAILHKCFDSPWTTRALSETVAEQSVRPLTFSKSKVLLSSSGCSADVDSNKQSLPYPQPGLESAGIESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQKDIIYRVYLKQIIVKVILFVLIITYVPYFLTYISLEIDCSVDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVILYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVEKLKSKLVKNSQDKIELHLFMLNGLPDNVFELTEIEVLSLELIPEVKLPSAVSQLVNLRELHVYHSSLVVDHPALGFLEENLKILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSTMQLEGFQDLKNLRTLYLKSSLSRIPQVITDLLPSLQKLALDNEGSKLVVLNNLKKMVNLKSLELISCDLERIPHSIFSLNNLHELDLKENNLKTVEEIISFQHLQNLSCLKLWHNNIAYIPAQIGALSNLEQLSLNHNNIENLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLSNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGKNSLMSLSPHVGELSNLTHLELIGNYLETLPPELEGCPSLKRSCLIVEENLLNTLPLPVTERLQTCLDKC

Target ORF information:

RefSeq Version	XM_005621953.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit B (LRRC8B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621953.3

ORF Insert Sequence:

ATGATTACAC TAACAGAGCT GAAATGTTTA GCAGACGCCC AGTCATCTTA TCACATCCTA 
AAACCATGGT GGGACGTCTT CTGGTATTAC ATTACCCTGA TCATGCTGCT GGTGGCTGTG 
CTGGCCGGAG CTCTCCAGCT CACACAGAGC AGGGTTCTGT GCTGTCTTCC ATGTAAGGTG 
GAATATGACA ATCACTGTGC CGTGCCTTGG GACATCCTGA GAGCCAGCGC AAACATGTCC 
TCTGATCCCG GGACACCGCT TCCGCTCCCC CTCAGAATCC AGAACGACCT CCACCGACAG 
CAGTACTCCT ACATTGACGC CGTCTGTTAT GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTACC TGGTGCTCTT GCACACGCTC ATCTTTGCAG CCTGTAGCAA CTTCTGGCTA 
CACTATCCCA GTACCAGTTC TCGGCTCGAG CATTTTGTGG CCATCCTTCA CAAGTGCTTC 
GATTCCCCGT GGACCACCCG GGCCCTGTCC GAAACGGTGG CCGAGCAATC AGTGAGGCCC 
CTGACATTCT CCAAGTCCAA GGTTCTGCTG TCGTCCTCAG GGTGCTCAGC CGACGTCGAT 
TCCAACAAGC AGTCATTGCC CTACCCACAG CCTGGCTTGG AGTCAGCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGTGAACAGG CCAAAGCCAT CTTTGAAAAA 
GTGAAAAGGT TCCGCCTGCA CGTGGAGCAG AAGGACATCA TTTATAGAGT GTATCTGAAG 
CAGATCATAG TGAAAGTCAT TTTGTTCGTC CTCATCATAA CTTATGTTCC GTATTTTCTA 
ACCTACATCA GTCTTGAAAT CGACTGTTCG GTTGATGTGC AAGCTTTCAC AGGCTACAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATATTTAAGG TCCTGGCTTC CTTTTATGTC 
ATTTTGGTTA TACTCTATGG TCTCACCTCC TCCTACAGCT TATGGTGGAT GCTGAGGAGT 
TCTCTGAAGC AATATTCCTT TGAGGCGTTG AGAGAGAAAA GCAACTATAG CGATATCCCC 
GACGTGAAGA ACGACTTCGC CTTCATCCTC CATCTGGCAG ATCAGTACGA TCCTCTTTAT 
TCCAAACGCT TCTCCATATT CCTGTCGGAG GTCAGCGAGA ACAAGCTGAA ACAGATCAAC 
CTCAACAACG AATGGACAGT TGAGAAGCTG AAGAGTAAGC TTGTGAAAAA CTCACAGGAC 
AAGATAGAGC TGCATCTTTT TATGCTAAAC GGTCTTCCGG ACAACGTCTT TGAGCTGACA 
GAAATCGAAG TGCTGAGCCT GGAGCTGATC CCTGAGGTCA AGCTGCCCTC TGCGGTCTCG 
CAGCTGGTCA ACCTCAGGGA GCTTCACGTG TACCACTCGT CCCTGGTGGT GGACCATCCA 
GCGCTGGGCT TCCTGGAGGA GAACCTAAAA ATCCTTCGCC TGAAATTCAC CGAAATGGGG 
AAGATCCCAC GCTGGGTATT TCACCTGAAG AATCTCAAGG AACTTTATCT GTCAGGCTGT 
GTTCTTCCGG AACAGCTGAG TACCATGCAG CTGGAGGGCT TCCAGGACTT GAAAAACCTG 
CGGACCCTCT ACCTGAAGAG CAGCCTCTCC CGGATCCCAC AGGTCATTAC AGACCTCCTG 
CCTTCTCTGC AGAAGCTGGC CCTCGATAAC GAGGGAAGCA AGCTGGTTGT GTTGAACAAC 
TTGAAAAAGA TGGTCAATCT GAAGAGCCTC GAGCTGATCA GCTGTGACCT GGAACGCATC 
CCACACTCTA TTTTCAGCCT CAACAATTTG CATGAGTTAG ACCTAAAAGA GAATAACCTT 
AAAACTGTGG AAGAGATCAT TAGCTTTCAG CATCTCCAGA ATCTTTCCTG CTTAAAGCTG 
TGGCATAATA ACATTGCTTA TATTCCTGCC CAGATTGGGG CACTATCTAA TCTAGAGCAG 
CTCTCTTTGA ACCATAATAA TATTGAGAAT CTGCCCCTGC AGCTTTTCCT ATGCACCAAA 
CTACATTATT TGGATCTAAG CTATAACCAC CTGACCTTCA TTCCAGAAGA AATCCAGTAT 
CTGAGTAATT TGCAGTACTT TGCTGTGACC AACAACAATA TTGAGATGCT ACCAGATGGG 
CTGTTTCAGT GCAAAAAGCT GCAGTGTTTA CTTTTGGGGA AAAATAGCCT GATGAGTTTA 
TCCCCTCACG TGGGTGAGCT GTCGAACCTT ACTCATCTGG AGCTCATTGG TAATTACCTG 
GAAACACTTC CTCCTGAACT GGAAGGCTGT CCGTCCCTAA AGCGGAGCTG TCTGATTGTA 
GAGGAGAACT TGCTCAACAC TCTTCCTCTC CCTGTGACAG AACGTTTGCA GACGTGCTTA 
GATAAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8B ( XM_005621953.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005622010.1 Canis lupus familiaris leucine rich repeat containing 8 family, member B (LRRC8B), mRNA.

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CloneID	OCe08621
Clone ID Related Accession (Same CDS sequence)	XM_022420527.1 , XM_005621953.3 , XM_005621953.2
Accession Version	XM_005621953.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	volume-regulated anion channel subunit LRRC8B
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACAC TAACAGAGCT GAAATGTTTA GCAGACGCCC AGTCATCTTA TCACATCCTA 
AAACCATGGT GGGACGTCTT CTGGTATTAC ATTACCCTGA TCATGCTGCT GGTGGCTGTG 
CTGGCCGGAG CTCTCCAGCT CACACAGAGC AGGGTTCTGT GCTGTCTTCC ATGTAAGGTG 
GAATATGACA ATCACTGTGC CGTGCCTTGG GACATCCTGA GAGCCAGCGC AAACATGTCC 
TCTGATCCCG GGACACCGCT TCCGCTCCCC CTCAGAATCC AGAACGACCT CCACCGACAG 
CAGTACTCCT ACATTGACGC CGTCTGTTAT GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTACC TGGTGCTCTT GCACACGCTC ATCTTTGCAG CCTGTAGCAA CTTCTGGCTA 
CACTATCCCA GTACCAGTTC TCGGCTCGAG CATTTTGTGG CCATCCTTCA CAAGTGCTTC 
GATTCCCCGT GGACCACCCG GGCCCTGTCC GAAACGGTGG CCGAGCAATC AGTGAGGCCC 
CTGACATTCT CCAAGTCCAA GGTTCTGCTG TCGTCCTCAG GGTGCTCAGC CGACGTCGAT 
TCCAACAAGC AGTCATTGCC CTACCCACAG CCTGGCTTGG AGTCAGCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGTGAACAGG CCAAAGCCAT CTTTGAAAAA 
GTGAAAAGGT TCCGCCTGCA CGTGGAGCAG AAGGACATCA TTTATAGAGT GTATCTGAAG 
CAGATCATAG TGAAAGTCAT TTTGTTCGTC CTCATCATAA CTTATGTTCC GTATTTTCTA 
ACCTACATCA GTCTTGAAAT CGACTGTTCG GTTGATGTGC AAGCTTTCAC AGGCTACAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATATTTAAGG TCCTGGCTTC CTTTTATGTC 
ATTTTGGTTA TACTCTATGG TCTCACCTCC TCCTACAGCT TATGGTGGAT GCTGAGGAGT 
TCTCTGAAGC AATATTCCTT TGAGGCGTTG AGAGAGAAAA GCAACTATAG CGATATCCCC 
GACGTGAAGA ACGACTTCGC CTTCATCCTC CATCTGGCAG ATCAGTACGA TCCTCTTTAT 
TCCAAACGCT TCTCCATATT CCTGTCGGAG GTCAGCGAGA ACAAGCTGAA ACAGATCAAC 
CTCAACAACG AATGGACAGT TGAGAAGCTG AAGAGTAAGC TTGTGAAAAA CTCACAGGAC 
AAGATAGAGC TGCATCTTTT TATGCTAAAC GGTCTTCCGG ACAACGTCTT TGAGCTGACA 
GAAATCGAAG TGCTGAGCCT GGAGCTGATC CCTGAGGTCA AGCTGCCCTC TGCGGTCTCG 
CAGCTGGTCA ACCTCAGGGA GCTTCACGTG TACCACTCGT CCCTGGTGGT GGACCATCCA 
GCGCTGGGCT TCCTGGAGGA GAACCTAAAA ATCCTTCGCC TGAAATTCAC CGAAATGGGG 
AAGATCCCAC GCTGGGTATT TCACCTGAAG AATCTCAAGG AACTTTATCT GTCAGGCTGT 
GTTCTTCCGG AACAGCTGAG TACCATGCAG CTGGAGGGCT TCCAGGACTT GAAAAACCTG 
CGGACCCTCT ACCTGAAGAG CAGCCTCTCC CGGATCCCAC AGGTCATTAC AGACCTCCTG 
CCTTCTCTGC AGAAGCTGGC CCTCGATAAC GAGGGAAGCA AGCTGGTTGT GTTGAACAAC 
TTGAAAAAGA TGGTCAATCT GAAGAGCCTC GAGCTGATCA GCTGTGACCT GGAACGCATC 
CCACACTCTA TTTTCAGCCT CAACAATTTG CATGAGTTAG ACCTAAAAGA GAATAACCTT 
AAAACTGTGG AAGAGATCAT TAGCTTTCAG CATCTCCAGA ATCTTTCCTG CTTAAAGCTG 
TGGCATAATA ACATTGCTTA TATTCCTGCC CAGATTGGGG CACTATCTAA TCTAGAGCAG 
CTCTCTTTGA ACCATAATAA TATTGAGAAT CTGCCCCTGC AGCTTTTCCT ATGCACCAAA 
CTACATTATT TGGATCTAAG CTATAACCAC CTGACCTTCA TTCCAGAAGA AATCCAGTAT 
CTGAGTAATT TGCAGTACTT TGCTGTGACC AACAACAATA TTGAGATGCT ACCAGATGGG 
CTGTTTCAGT GCAAAAAGCT GCAGTGTTTA CTTTTGGGGA AAAATAGCCT GATGAGTTTA 
TCCCCTCACG TGGGTGAGCT GTCGAACCTT ACTCATCTGG AGCTCATTGG TAATTACCTG 
GAAACACTTC CTCCTGAACT GGAAGGCTGT CCGTCCCTAA AGCGGAGCTG TCTGATTGTA 
GAGGAGAACT TGCTCAACAC TCTTCCTCTC CCTGTGACAG AACGTTTGCA GACGTGCTTA 
GATAAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622010.1
CDS	214..2625
Translation	MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEYDNHCAVPWDILRASANMSSDPGTPLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVAILHKCFDSPWTTRALSETVAEQSVRPLTFSKSKVLLSSSGCSADVDSNKQSLPYPQPGLESAGIESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQKDIIYRVYLKQIIVKVILFVLIITYVPYFLTYISLEIDCSVDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVILYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVEKLKSKLVKNSQDKIELHLFMLNGLPDNVFELTEIEVLSLELIPEVKLPSAVSQLVNLRELHVYHSSLVVDHPALGFLEENLKILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSTMQLEGFQDLKNLRTLYLKSSLSRIPQVITDLLPSLQKLALDNEGSKLVVLNNLKKMVNLKSLELISCDLERIPHSIFSLNNLHELDLKENNLKTVEEIISFQHLQNLSCLKLWHNNIAYIPAQIGALSNLEQLSLNHNNIENLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLSNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGKNSLMSLSPHVGELSNLTHLELIGNYLETLPPELEGCPSLKRSCLIVEENLLNTLPLPVTERLQTCLDKC

Target ORF information:

RefSeq Version	XM_005621953.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 family, member B (LRRC8B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621953.2

ORF Insert Sequence:

ATGATTACAC TAACAGAGCT GAAATGTTTA GCAGACGCCC AGTCATCTTA TCACATCCTA 
AAACCATGGT GGGACGTCTT CTGGTATTAC ATTACCCTGA TCATGCTGCT GGTGGCTGTG 
CTGGCCGGAG CTCTCCAGCT CACACAGAGC AGGGTTCTGT GCTGTCTTCC ATGTAAGGTG 
GAATATGACA ATCACTGTGC CGTGCCTTGG GACATCCTGA GAGCCAGCGC AAACATGTCC 
TCTGATCCCG GGACACCGCT TCCGCTCCCC CTCAGAATCC AGAACGACCT CCACCGACAG 
CAGTACTCCT ACATTGACGC CGTCTGTTAT GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTACC TGGTGCTCTT GCACACGCTC ATCTTTGCAG CCTGTAGCAA CTTCTGGCTA 
CACTATCCCA GTACCAGTTC TCGGCTCGAG CATTTTGTGG CCATCCTTCA CAAGTGCTTC 
GATTCCCCGT GGACCACCCG GGCCCTGTCC GAAACGGTGG CCGAGCAATC AGTGAGGCCC 
CTGACATTCT CCAAGTCCAA GGTTCTGCTG TCGTCCTCAG GGTGCTCAGC CGACGTCGAT 
TCCAACAAGC AGTCATTGCC CTACCCACAG CCTGGCTTGG AGTCAGCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGTGAACAGG CCAAAGCCAT CTTTGAAAAA 
GTGAAAAGGT TCCGCCTGCA CGTGGAGCAG AAGGACATCA TTTATAGAGT GTATCTGAAG 
CAGATCATAG TGAAAGTCAT TTTGTTCGTC CTCATCATAA CTTATGTTCC GTATTTTCTA 
ACCTACATCA GTCTTGAAAT CGACTGTTCG GTTGATGTGC AAGCTTTCAC AGGCTACAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATATTTAAGG TCCTGGCTTC CTTTTATGTC 
ATTTTGGTTA TACTCTATGG TCTCACCTCC TCCTACAGCT TATGGTGGAT GCTGAGGAGT 
TCTCTGAAGC AATATTCCTT TGAGGCGTTG AGAGAGAAAA GCAACTATAG CGATATCCCC 
GACGTGAAGA ACGACTTCGC CTTCATCCTC CATCTGGCAG ATCAGTACGA TCCTCTTTAT 
TCCAAACGCT TCTCCATATT CCTGTCGGAG GTCAGCGAGA ACAAGCTGAA ACAGATCAAC 
CTCAACAACG AATGGACAGT TGAGAAGCTG AAGAGTAAGC TTGTGAAAAA CTCACAGGAC 
AAGATAGAGC TGCATCTTTT TATGCTAAAC GGTCTTCCGG ACAACGTCTT TGAGCTGACA 
GAAATCGAAG TGCTGAGCCT GGAGCTGATC CCTGAGGTCA AGCTGCCCTC TGCGGTCTCG 
CAGCTGGTCA ACCTCAGGGA GCTTCACGTG TACCACTCGT CCCTGGTGGT GGACCATCCA 
GCGCTGGGCT TCCTGGAGGA GAACCTAAAA ATCCTTCGCC TGAAATTCAC CGAAATGGGG 
AAGATCCCAC GCTGGGTATT TCACCTGAAG AATCTCAAGG AACTTTATCT GTCAGGCTGT 
GTTCTTCCGG AACAGCTGAG TACCATGCAG CTGGAGGGCT TCCAGGACTT GAAAAACCTG 
CGGACCCTCT ACCTGAAGAG CAGCCTCTCC CGGATCCCAC AGGTCATTAC AGACCTCCTG 
CCTTCTCTGC AGAAGCTGGC CCTCGATAAC GAGGGAAGCA AGCTGGTTGT GTTGAACAAC 
TTGAAAAAGA TGGTCAATCT GAAGAGCCTC GAGCTGATCA GCTGTGACCT GGAACGCATC 
CCACACTCTA TTTTCAGCCT CAACAATTTG CATGAGTTAG ACCTAAAAGA GAATAACCTT 
AAAACTGTGG AAGAGATCAT TAGCTTTCAG CATCTCCAGA ATCTTTCCTG CTTAAAGCTG 
TGGCATAATA ACATTGCTTA TATTCCTGCC CAGATTGGGG CACTATCTAA TCTAGAGCAG 
CTCTCTTTGA ACCATAATAA TATTGAGAAT CTGCCCCTGC AGCTTTTCCT ATGCACCAAA 
CTACATTATT TGGATCTAAG CTATAACCAC CTGACCTTCA TTCCAGAAGA AATCCAGTAT 
CTGAGTAATT TGCAGTACTT TGCTGTGACC AACAACAATA TTGAGATGCT ACCAGATGGG 
CTGTTTCAGT GCAAAAAGCT GCAGTGTTTA CTTTTGGGGA AAAATAGCCT GATGAGTTTA 
TCCCCTCACG TGGGTGAGCT GTCGAACCTT ACTCATCTGG AGCTCATTGG TAATTACCTG 
GAAACACTTC CTCCTGAACT GGAAGGCTGT CCGTCCCTAA AGCGGAGCTG TCTGATTGTA 
GAGGAGAACT TGCTCAACAC TCTTCCTCTC CCTGTGACAG AACGTTTGCA GACGTGCTTA 
GATAAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8B ( XM_022420528.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022276236.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit B (LRRC8B), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe08623
Clone ID Related Accession (Same CDS sequence)	XM_022420528.1
Accession Version	XM_022420528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACAC TAACAGAGCT GAAATGTTTA GCAGACGCCC AGTCATCTTA TCACATCCTA 
AAACCATGGT GGGACGTCTT CTGGTATTAC ATTACCCTGA TCATGCTGCT GGTGGCTGTG 
CTGGCCGGAG CTCTCCAGCT CACACAGAGC AGGGTTCTGT GCTGTCTTCC ATGTAAGGTG 
GAATATGACA ATCACTGTGC CGTGCCTTGG GACATCCTGA GAGCCAGCGC AAACATGTCC 
TCTGATCCCG GGACACCGCT TCCGCTCCCC CTCAGAATCC AGAACGACCT CCACCGACAG 
CAGTACTCCT ACATTGACGC CGTCTGTTAT GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTACC TGGTGCTCTT GCACACGCTC ATCTTTGCAG CCTGTAGCAA CTTCTGGCTA 
CACTATCCCA GTACCAGTTC TCGGCTCGAG CATTTTGTGG CCATCCTTCA CAAGTGCTTC 
GATTCCCCGT GGACCACCCG GGCCCTGTCC GAAACGGTGG CCGAGCAATC AGTGAGGCCC 
CTGACATTCT CCAAGTCCAA GGTTCTGCTG TCGTCCTCAG GGTGCTCAGC CGACGTCGAT 
TCCAACAAGC AGTCATTGCC CTACCCACAG CCTGGCTTGG AGTCAGCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGTGAACAGG CCAAAGCCAT CTTTGAAAAA 
GTGAAAAGGT TCCGCCTGCA CGTGGAGCAG AAGGACATCA TTTATAGAGT GTATCTGAAG 
CAGATCATAG TGAAAGTCAT TTTGTTCGTC CTCATCATAA CTTATGTTCC GTATTTTCTA 
ACCTACATCA GTCTTGAAAT CGACTGTTCG GTTGATGTGC AAGCTTTCAC AGGCTACAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATATTTAAGG TCCTGGCTTC CTTTTATGTC 
ATTTTGGTTA TACTCTATGG TCTCACCTCC TCCTACAGCT TATGGTGGAT GCTGAGGAGT 
TCTCTGAAGC AATATTCCTT TGAGGCGTTG AGAGAGAAAA GCAACTATAG CGATATCCCC 
GACGTGAAGA ACGACTTCGC CTTCATCCTC CATCTGGCAG ATCAGTACGA TCCTCTTTAT 
TCCAAACGCT TCTCCATATT CCTGTCGGAG GTCAGCGAGA ACAAGCTGAA ACAGATCAAC 
CTCAACAACG AATGGACAGT TGAGAAGCTG AAGAGTAAGC TTGTGAAAAA CTCACAGGAC 
AAGATAGAGC TGCATCTTTT TATGCTAAAC GGTCTTCCGG ACAACGTCTT TGAGCTGACA 
GAAATCGAAG TGCTGAGCCT GGAGCTGATC CCTGAGGTCA AGCTGCCCTC TGCGGTCTCG 
CAGCTGGTCA ACCTCAGGGA GCTTCACGTG TACCACTCGT CCCTGGTGGT GGACCATCCA 
GCGCTGGGCT TCCTGGAGGA GAACCTAAAA ATCCTTCGCC TGAAATTCAC CGAAATGGGG 
AAGATCCCAC GCTGGGTATT TCACCTGAAG AATCTCAAGG AACTTTATCT GTCAGGCTGT 
GTTCTTCCGG AACAGCTGAG TACCATGCAG CTGGAGGGCT TCCAGGACTT GAAAAACCTG 
CGGACCCTCT ACCTGAAGAG CAGCCTCTCC CGGATCCCAC AGGTCATTAC AGACCTCCTG 
CCTTCTCTGC AGAAGCTGGC CCTCGATAAC GAGGGAAGCA AGCTGGTTGT GTTGAACAAC 
TTGAAAAAGA TGGTCAATCT GAAGAGCCTC GAGCTGATCA GCTGTGACCT GGAACGCATC 
CCACACTCTA TTTTCAGCCT CAACAATTTG CATGAGTTAG ACCTAAAAGA GAATAACCTT 
AAAACTGTGG AAGAGATCAT TAGCTTTCAG CATCTCCAGA ATCTTTCCTG CTTAAAGCTG 
TGGCATAATA ACATTGCTTA TATTCCTGCC CAGATTGGGG CACTATCTAA TCTAGAGCAG 
CTCTCTTTGA ACCATAATAA TATTGAGAAT CTGCCCCTGC AGCTTTTCCT ATGCACCAAA 
CTACATTATT TGGATCTAAG CTATAACCAC CTGACCTTCA TTCCAGAAGA AATCCAGTAT 
CTGAGTAATT TGCAGTACTT TGCTGTGACC AACAACAATA TTGAGATGCT ACCAGATGGG 
CTGTTTCAGT GCAAAAAGCT GCAGTGTTTA CTTTTGGGGA AAAATAGCCT GATGAGTTTA 
TCCCCTCACG TGGGTGAGCT GTCGAACCTT ACTCATCTGG AGCTCATTGG GGCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276236.1
CDS	244..2523
Translation	MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEYDNHCAVPWDILRASANMSSDPGTPLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVAILHKCFDSPWTTRALSETVAEQSVRPLTFSKSKVLLSSSGCSADVDSNKQSLPYPQPGLESAGIESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQKDIIYRVYLKQIIVKVILFVLIITYVPYFLTYISLEIDCSVDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVILYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVEKLKSKLVKNSQDKIELHLFMLNGLPDNVFELTEIEVLSLELIPEVKLPSAVSQLVNLRELHVYHSSLVVDHPALGFLEENLKILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSTMQLEGFQDLKNLRTLYLKSSLSRIPQVITDLLPSLQKLALDNEGSKLVVLNNLKKMVNLKSLELISCDLERIPHSIFSLNNLHELDLKENNLKTVEEIISFQHLQNLSCLKLWHNNIAYIPAQIGALSNLEQLSLNHNNIENLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLSNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGKNSLMSLSPHVGELSNLTHLELIGAH

Target ORF information:

RefSeq Version	XM_022420528.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit B (LRRC8B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420528.1

ORF Insert Sequence:

ATGATTACAC TAACAGAGCT GAAATGTTTA GCAGACGCCC AGTCATCTTA TCACATCCTA 
AAACCATGGT GGGACGTCTT CTGGTATTAC ATTACCCTGA TCATGCTGCT GGTGGCTGTG 
CTGGCCGGAG CTCTCCAGCT CACACAGAGC AGGGTTCTGT GCTGTCTTCC ATGTAAGGTG 
GAATATGACA ATCACTGTGC CGTGCCTTGG GACATCCTGA GAGCCAGCGC AAACATGTCC 
TCTGATCCCG GGACACCGCT TCCGCTCCCC CTCAGAATCC AGAACGACCT CCACCGACAG 
CAGTACTCCT ACATTGACGC CGTCTGTTAT GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTACC TGGTGCTCTT GCACACGCTC ATCTTTGCAG CCTGTAGCAA CTTCTGGCTA 
CACTATCCCA GTACCAGTTC TCGGCTCGAG CATTTTGTGG CCATCCTTCA CAAGTGCTTC 
GATTCCCCGT GGACCACCCG GGCCCTGTCC GAAACGGTGG CCGAGCAATC AGTGAGGCCC 
CTGACATTCT CCAAGTCCAA GGTTCTGCTG TCGTCCTCAG GGTGCTCAGC CGACGTCGAT 
TCCAACAAGC AGTCATTGCC CTACCCACAG CCTGGCTTGG AGTCAGCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGTGAACAGG CCAAAGCCAT CTTTGAAAAA 
GTGAAAAGGT TCCGCCTGCA CGTGGAGCAG AAGGACATCA TTTATAGAGT GTATCTGAAG 
CAGATCATAG TGAAAGTCAT TTTGTTCGTC CTCATCATAA CTTATGTTCC GTATTTTCTA 
ACCTACATCA GTCTTGAAAT CGACTGTTCG GTTGATGTGC AAGCTTTCAC AGGCTACAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATATTTAAGG TCCTGGCTTC CTTTTATGTC 
ATTTTGGTTA TACTCTATGG TCTCACCTCC TCCTACAGCT TATGGTGGAT GCTGAGGAGT 
TCTCTGAAGC AATATTCCTT TGAGGCGTTG AGAGAGAAAA GCAACTATAG CGATATCCCC 
GACGTGAAGA ACGACTTCGC CTTCATCCTC CATCTGGCAG ATCAGTACGA TCCTCTTTAT 
TCCAAACGCT TCTCCATATT CCTGTCGGAG GTCAGCGAGA ACAAGCTGAA ACAGATCAAC 
CTCAACAACG AATGGACAGT TGAGAAGCTG AAGAGTAAGC TTGTGAAAAA CTCACAGGAC 
AAGATAGAGC TGCATCTTTT TATGCTAAAC GGTCTTCCGG ACAACGTCTT TGAGCTGACA 
GAAATCGAAG TGCTGAGCCT GGAGCTGATC CCTGAGGTCA AGCTGCCCTC TGCGGTCTCG 
CAGCTGGTCA ACCTCAGGGA GCTTCACGTG TACCACTCGT CCCTGGTGGT GGACCATCCA 
GCGCTGGGCT TCCTGGAGGA GAACCTAAAA ATCCTTCGCC TGAAATTCAC CGAAATGGGG 
AAGATCCCAC GCTGGGTATT TCACCTGAAG AATCTCAAGG AACTTTATCT GTCAGGCTGT 
GTTCTTCCGG AACAGCTGAG TACCATGCAG CTGGAGGGCT TCCAGGACTT GAAAAACCTG 
CGGACCCTCT ACCTGAAGAG CAGCCTCTCC CGGATCCCAC AGGTCATTAC AGACCTCCTG 
CCTTCTCTGC AGAAGCTGGC CCTCGATAAC GAGGGAAGCA AGCTGGTTGT GTTGAACAAC 
TTGAAAAAGA TGGTCAATCT GAAGAGCCTC GAGCTGATCA GCTGTGACCT GGAACGCATC 
CCACACTCTA TTTTCAGCCT CAACAATTTG CATGAGTTAG ACCTAAAAGA GAATAACCTT 
AAAACTGTGG AAGAGATCAT TAGCTTTCAG CATCTCCAGA ATCTTTCCTG CTTAAAGCTG 
TGGCATAATA ACATTGCTTA TATTCCTGCC CAGATTGGGG CACTATCTAA TCTAGAGCAG 
CTCTCTTTGA ACCATAATAA TATTGAGAAT CTGCCCCTGC AGCTTTTCCT ATGCACCAAA 
CTACATTATT TGGATCTAAG CTATAACCAC CTGACCTTCA TTCCAGAAGA AATCCAGTAT 
CTGAGTAATT TGCAGTACTT TGCTGTGACC AACAACAATA TTGAGATGCT ACCAGATGGG 
CTGTTTCAGT GCAAAAAGCT GCAGTGTTTA CTTTTGGGGA AAAATAGCCT GATGAGTTTA 
TCCCCTCACG TGGGTGAGCT GTCGAACCTT ACTCATCTGG AGCTCATTGG GGCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LRRC8B cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム