HFM1 cDNA ORF clone, Canis lupus familiaris(dog)

The following HFM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HFM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe289265 XM_022420500.1
Latest version!
Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe289265 XM_022420501.1
Latest version!
Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe289265 XM_022420502.1
Latest version!
Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe289266 XM_022420503.1
Latest version!
Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe289267 XM_022420504.1
Latest version!
Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe289268 XM_022420505.1
Latest version!
Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe289269 XM_022420506.1
Latest version!
Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe289270 XM_022420507.1
Latest version!
Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe289271 XM_022420508.1
Latest version!
Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe37267 XM_014114844.1
Latest version!
Canis lupus familiaris HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe08612 XM_005621940.1
Latest version!
Canis lupus familiaris HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe08610 XM_005621938.2
Latest version!
Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe08610 XM_005621939.2
Latest version!
Canis lupus familiaris HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe08610 XM_005621938.1 Canis lupus familiaris HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe289265
    Clone ID Related Accession (Same CDS sequence) XM_022420502.1 , XM_022420500.1 , XM_022420501.1
    Accession Version XM_022420502.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGTAGGTATC ATCCTGATGA TGAAGCTGAT GAAATGAAGT CTTTCCTGGG AATATTTGAT
    GACATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276210.1
    CDS148..4599
    Translation

    Target ORF information:

    RefSeq Version XM_022420502.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420502.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGTAGGTATC ATCCTGATGA TGAAGCTGAT GAAATGAAGT CTTTCCTGGG AATATTTGAT
    GACATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289265
    Clone ID Related Accession (Same CDS sequence) XM_022420502.1 , XM_022420500.1 , XM_022420501.1
    Accession Version XM_022420500.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGTAGGTATC ATCCTGATGA TGAAGCTGAT GAAATGAAGT CTTTCCTGGG AATATTTGAT
    GACATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276208.1
    CDS148..4599
    Translation

    Target ORF information:

    RefSeq Version XM_022420500.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420500.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGTAGGTATC ATCCTGATGA TGAAGCTGAT GAAATGAAGT CTTTCCTGGG AATATTTGAT
    GACATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289265
    Clone ID Related Accession (Same CDS sequence) XM_022420502.1 , XM_022420500.1 , XM_022420501.1
    Accession Version XM_022420501.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGTAGGTATC ATCCTGATGA TGAAGCTGAT GAAATGAAGT CTTTCCTGGG AATATTTGAT
    GACATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276209.1
    CDS375..4826
    Translation

    Target ORF information:

    RefSeq Version XM_022420501.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420501.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGTAGGTATC ATCCTGATGA TGAAGCTGAT GAAATGAAGT CTTTCCTGGG AATATTTGAT
    GACATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289266
    Clone ID Related Accession (Same CDS sequence) XM_022420503.1
    Accession Version XM_022420503.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4449bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGGTATCATC CTGATGATGA AGCTGATGAA ATGAAGTCTT TCCTGGGAAT ATTTGATGAC
    ATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276211.1
    CDS149..4597
    Translation

    Target ORF information:

    RefSeq Version XM_022420503.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420503.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGGTATCATC CTGATGATGA AGCTGATGAA ATGAAGTCTT TCCTGGGAAT ATTTGATGAC
    ATTTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289267
    Clone ID Related Accession (Same CDS sequence) XM_022420504.1
    Accession Version XM_022420504.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGCATGTCAA TTCCTGGCTG CTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276212.1
    CDS149..4555
    Translation

    Target ORF information:

    RefSeq Version XM_022420504.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420504.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGCATGTCAA TTCCTGGCTG CTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289268
    Clone ID Related Accession (Same CDS sequence) XM_022420505.1
    Accession Version XM_022420505.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGA ATTATGTTTG
    AAGAATCTGC ATGATTTATC ATCTCTGGAC TTGATAAGGA TGGATGAAGA TGTTAATTTC
    AAACCTACTG AGGCAGGAAG ATTGATGGCT TGGTATTACA TTACATTTGA GACAGTGAAG
    AAATTTTGTA CTATCAGTGG AAAAGAGACT TTATTAGATC TGGTCACGAT GATATCCAGC
    TGCAAGGAAT TTGTAGATGT GCAGTTAAGG ATAAATGAAA AGAAAATATT GAATACTTTG
    AACAAAGATC CAAATAGAGT AACTATCAGA TTTCCAATGG AAGGAAGAAT TAAAACAAGA
    GAAATGAAAA TAAATTGTCT TATACAGGCT CAACTAGGAT GCATTCCCAT ACAAGACTTT
    GCTTTGACAC AAGATATCTC GAAGATTTTT AGAAATGGCT CACGAATTAC AAGATGGTTG
    TCAGATTTTG TGGCTGTTCA AGAAAAGTTT GCTATATTAT TGAACAGTTT GATTTTAGCT
    AAATGTTTTA GATGCAAACT TTGGGAAAAC TCTCTACATG TATCCAAACA GCTGGAAAAA
    ATAGGTATAA CATTGTCAAA TGCAATGGTA AATGCAGGTT TGACTTCCTT TAAAAAAATA
    GAAGAAACAG ATGCAAGGGA ACTTGAACTG ATTTTAAATA GACACCCCCC TTTTGGAACG
    CAAATAAAAG AAACAGTGAT GTATCTACCA AAATATGAAG TTGAAGTGGA ACAGATTGCA
    AGATACAGTG ATACCATGGC AGAAATATTA GTGACAATTA TATTAAGAAA CTTTGAACAG
    CTACAAGCTA GAAGAACATC ACCAGATTCT CACTATGTTA CCTTAATCAT AGGTGATGCA
    GATAACCAGC TGGTTTTTAA GCACAAAATT ATGGATTCTG TTTTATTAAA AGCTGGAAAT
    TGGACTAAAA AGATTGATGT GAGGAGAGCT TTTAAATCTG AAGATCTTAG CATAAATCTA
    ATTAGTTCTG AGTATGTTGG ACTTGATATT CAGCAGAAAT TTACAGTCTT TTACTTAGGA
    CCCAGGAGGC TTGGAAATCA AATAATTATG CAAAAAAAAT CAGAAACAGA GATTTCCCAT
    TCTAAACATT CAAATAGATC TACTATAGCA GGACCCAATA AAGATTTAGG AATGACTGCT
    TGCAGAAAAC CTGGGAACCG AGAATGTAAT CATCACTGTA AAAATAAACA CACATGTGGA
    CATGACTGCT GTAAAACTGG AGTGGCACAA AAGTCAGAAA CAAAAGAGCC AACAATCTCT
    TCATATTTAT CTGATTTAAG AAACAGGAAT GCTGTTTCAT CACTTCCTCC AGTTAAACGT
    CTCAAGATGC AAATGAATAA ATCTCGAAGT GTGGACCTTA AAGAATTTGG TTTTACTCCA
    AAACCTTCTG TTCCCAGTAT ATCAAGGTCA GAGTGTTTAA ACATACCTGG ATTGTCTGTA
    ATGGAGGAGA GGGATCAGCG TGATTTCTGT GTAAAAGTTC AACAAGAACC GTCTGAATAT
    GAAGACAAAG ATTTCTCTAG ATGGAACTAT TCTGCAACTT CAAATGAGAA ATCCCCTCAA
    ACAGTGCTGT TCAACAGACA TCTTAATGCA TCTGGAAACC AAAATTTGAC AGATGGGGAT
    AAAAGTAATT CTCCATCCTC AGAAGTTTTG AATGTGAACT TTGAATTGGG AAATGAAGTT
    TGGGATGATT TTGATGATGA GAACTTATTA GAAGTTACTA GTTTTTCAGC CAGTACTGAG
    AAGACAAAAA CATCAGGATT TGGAAACACT TTGAGTTCAA GTACCAGGGG AAGTAAGCTA
    TTGCTCCAAG AGCCAAAGAG CAAATTCCAA GGAGAAATGA CAAAAAGTTT TGTTTCATCA
    CATGGGAAGC CAGATACTTA TTTATCAAAT TCTCCTGTAT TCGAGTTTGA TACATCCTCC
    CTGACGAAAT TACCTCAACA AGCTGGAAAT ACAACTATTG TCCATTCACA AGAAAGAAAA
    CAACAAAATC TGTCACCAGA GATTGGAAAG CTATGCTTTA CTCACTCTAA AAAAATACCA
    AATTCTTCAC ATTTTGTTAA GAAAGTGGAT TTCTTTATTA GAAACAATGA ATGTAAAGAG
    GAAATTGATT TCAGTAGGTA TCATCCTGAT GATGAAGCTG ATGAAATGAA GTCTTTCCTG
    GGAATATTTG ATGACATTTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276213.1
    CDS148..4551
    Translation

    Target ORF information:

    RefSeq Version XM_022420505.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420505.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGA ATTATGTTTG
    AAGAATCTGC ATGATTTATC ATCTCTGGAC TTGATAAGGA TGGATGAAGA TGTTAATTTC
    AAACCTACTG AGGCAGGAAG ATTGATGGCT TGGTATTACA TTACATTTGA GACAGTGAAG
    AAATTTTGTA CTATCAGTGG AAAAGAGACT TTATTAGATC TGGTCACGAT GATATCCAGC
    TGCAAGGAAT TTGTAGATGT GCAGTTAAGG ATAAATGAAA AGAAAATATT GAATACTTTG
    AACAAAGATC CAAATAGAGT AACTATCAGA TTTCCAATGG AAGGAAGAAT TAAAACAAGA
    GAAATGAAAA TAAATTGTCT TATACAGGCT CAACTAGGAT GCATTCCCAT ACAAGACTTT
    GCTTTGACAC AAGATATCTC GAAGATTTTT AGAAATGGCT CACGAATTAC AAGATGGTTG
    TCAGATTTTG TGGCTGTTCA AGAAAAGTTT GCTATATTAT TGAACAGTTT GATTTTAGCT
    AAATGTTTTA GATGCAAACT TTGGGAAAAC TCTCTACATG TATCCAAACA GCTGGAAAAA
    ATAGGTATAA CATTGTCAAA TGCAATGGTA AATGCAGGTT TGACTTCCTT TAAAAAAATA
    GAAGAAACAG ATGCAAGGGA ACTTGAACTG ATTTTAAATA GACACCCCCC TTTTGGAACG
    CAAATAAAAG AAACAGTGAT GTATCTACCA AAATATGAAG TTGAAGTGGA ACAGATTGCA
    AGATACAGTG ATACCATGGC AGAAATATTA GTGACAATTA TATTAAGAAA CTTTGAACAG
    CTACAAGCTA GAAGAACATC ACCAGATTCT CACTATGTTA CCTTAATCAT AGGTGATGCA
    GATAACCAGC TGGTTTTTAA GCACAAAATT ATGGATTCTG TTTTATTAAA AGCTGGAAAT
    TGGACTAAAA AGATTGATGT GAGGAGAGCT TTTAAATCTG AAGATCTTAG CATAAATCTA
    ATTAGTTCTG AGTATGTTGG ACTTGATATT CAGCAGAAAT TTACAGTCTT TTACTTAGGA
    CCCAGGAGGC TTGGAAATCA AATAATTATG CAAAAAAAAT CAGAAACAGA GATTTCCCAT
    TCTAAACATT CAAATAGATC TACTATAGCA GGACCCAATA AAGATTTAGG AATGACTGCT
    TGCAGAAAAC CTGGGAACCG AGAATGTAAT CATCACTGTA AAAATAAACA CACATGTGGA
    CATGACTGCT GTAAAACTGG AGTGGCACAA AAGTCAGAAA CAAAAGAGCC AACAATCTCT
    TCATATTTAT CTGATTTAAG AAACAGGAAT GCTGTTTCAT CACTTCCTCC AGTTAAACGT
    CTCAAGATGC AAATGAATAA ATCTCGAAGT GTGGACCTTA AAGAATTTGG TTTTACTCCA
    AAACCTTCTG TTCCCAGTAT ATCAAGGTCA GAGTGTTTAA ACATACCTGG ATTGTCTGTA
    ATGGAGGAGA GGGATCAGCG TGATTTCTGT GTAAAAGTTC AACAAGAACC GTCTGAATAT
    GAAGACAAAG ATTTCTCTAG ATGGAACTAT TCTGCAACTT CAAATGAGAA ATCCCCTCAA
    ACAGTGCTGT TCAACAGACA TCTTAATGCA TCTGGAAACC AAAATTTGAC AGATGGGGAT
    AAAAGTAATT CTCCATCCTC AGAAGTTTTG AATGTGAACT TTGAATTGGG AAATGAAGTT
    TGGGATGATT TTGATGATGA GAACTTATTA GAAGTTACTA GTTTTTCAGC CAGTACTGAG
    AAGACAAAAA CATCAGGATT TGGAAACACT TTGAGTTCAA GTACCAGGGG AAGTAAGCTA
    TTGCTCCAAG AGCCAAAGAG CAAATTCCAA GGAGAAATGA CAAAAAGTTT TGTTTCATCA
    CATGGGAAGC CAGATACTTA TTTATCAAAT TCTCCTGTAT TCGAGTTTGA TACATCCTCC
    CTGACGAAAT TACCTCAACA AGCTGGAAAT ACAACTATTG TCCATTCACA AGAAAGAAAA
    CAACAAAATC TGTCACCAGA GATTGGAAAG CTATGCTTTA CTCACTCTAA AAAAATACCA
    AATTCTTCAC ATTTTGTTAA GAAAGTGGAT TTCTTTATTA GAAACAATGA ATGTAAAGAG
    GAAATTGATT TCAGTAGGTA TCATCCTGAT GATGAAGCTG ATGAAATGAA GTCTTTCCTG
    GGAATATTTG ATGACATTTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289269
    Clone ID Related Accession (Same CDS sequence) XM_022420506.1
    Accession Version XM_022420506.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGTAGGTATC ATCCTGATGA TGAAGCTGAT GAAATGAAGT CTTTCCTGGG AATATTTGAT
    GACATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276214.1
    CDS148..4539
    Translation

    Target ORF information:

    RefSeq Version XM_022420506.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420506.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GATTTAGGAA TGACTGCTTG CAGAAAACCT
    GGGAACCGAG AATGTAATCA TCACTGTAAA AATAAACACA CATGTGGACA TGACTGCTGT
    AAAACTGGAG TGGCACAAAA GTCAGAAACA AAAGAGCCAA CAATCTCTTC ATATTTATCT
    GATTTAAGAA ACAGGAATGC TGTTTCATCA CTTCCTCCAG TTAAACGTCT CAAGATGCAA
    ATGAATAAAT CTCGAAGTGT GGACCTTAAA GAATTTGGTT TTACTCCAAA ACCTTCTGTT
    CCCAGTATAT CAAGGTCAGA GTGTTTAAAC ATACCTGGAT TGTCTGTAAT GGAGGAGAGG
    GATCAGCGTG ATTTCTGTGT AAAAGTTCAA CAAGAACCGT CTGAATATGA AGACAAAGAT
    TTCTCTAGAT GGAACTATTC TGCAACTTCA AATGAGAAAT CCCCTCAAAC AGTGCTGTTC
    AACAGACATC TTAATGCATC TGGAAACCAA AATTTGACAG ATGGGGATAA AAGTAATTCT
    CCATCCTCAG AAGTTTTGAA TGTGAACTTT GAATTGGGAA ATGAAGTTTG GGATGATTTT
    GATGATGAGA ACTTATTAGA AGTTACTAGT TTTTCAGCCA GTACTGAGAA GACAAAAACA
    TCAGGATTTG GAAACACTTT GAGTTCAAGT ACCAGGGGAA GTAAGCTATT GCTCCAAGAG
    CCAAAGAGCA AATTCCAAGG AGAAATGACA AAAAGTTTTG TTTCATCACA TGGGAAGCCA
    GATACTTATT TATCAAATTC TCCTGTATTC GAGTTTGATA CATCCTCCCT GACGAAATTA
    CCTCAACAAG CTGGAAATAC AACTATTGTC CATTCACAAG AAAGAAAACA ACAAAATCTG
    TCACCAGAGA TTGGAAAGCT ATGCTTTACT CACTCTAAAA AAATACCAAA TTCTTCACAT
    TTTGTTAAGA AAGTGGATTT CTTTATTAGA AACAATGAAT GTAAAGAGGA AATTGATTTC
    AGTAGGTATC ATCCTGATGA TGAAGCTGAT GAAATGAAGT CTTTCCTGGG AATATTTGAT
    GACATTTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289270
    Clone ID Related Accession (Same CDS sequence) XM_022420507.1
    Accession Version XM_022420507.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGACTCCTT GTTTTCTTTG GATACTTTGT TTTTTGAAAA ACCAGATGAA GCTGAAAAGT 
    CAGGAAGAAA GGCCAAAAAT GTTACCATCA AATTTAAAGA TGACTAATGA AAACACAAAT
    TATATTTCAC CAACACAAAA ATTCCAATTT GCCTTTTCCT CAAATAAATG TGAAACAGGT
    GATCTGAATT TAACAGAAGC AGGTAACCAT GACTTACCAC GTGTTCCTGG GAAGCTGACC
    TATGGTTCTC AGAAATACAA AAATCACATT GGCACCGAGA TAGCACCTGA GAAAAATGTT
    TCAGGTAATA CAAAATTAGT AAATTTTGCA GAAGATAAAG GAGAGAGCAC ATCAGCCTTC
    CGAAAAAGAT TATTTAAAAT ATCTGACAAT ATACATGAGG GTGTTTATTC TAATGACAGT
    ACAAACTTGG AGTCTTCTGT TGGCTCAGTG AAAATTGTCC CAACAGAAAT GAACAAAGGG
    GAATCATGGA AATGTAGCAA TAGTAAGCAG AAACATCAAT ATTCAGACAT TAATATGTTT
    ACAGCAAGCA ATGCTGTTTC AGCTTCTGAA ATCAGAGAAG ACATATTCAA AGGACCAACT
    TTTTCAGTTG CATCCCAACC TCATGAAGTT CAAGGGGTAA CAGAAAATGA TTTAGATTCT
    TTGAAGGCTG TCACAGAAAT CCCTGCAAAA TTTAGAAGTA TTTTCAAAGA ATTCCCATAT
    TTCAACTATA TACAGTCCAA AGCTTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGGAAAACT GTGGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTCAAAC ATAAAAATTG TTTATATGGC ACCAATAAAA
    GCCCTGTGTA GTCAGCGTTT CGATGATTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TCACTGGAGA TACAGTAATG GATGACCTAT TTGAGATCCA ACATGCCCAT
    ATTATTATGA CAACTCCCGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTCCAGC TGGTTAGACT CTTTCTCATT GATGAGGTAC ATGTTGTAAA AGATGAAAAT
    CGGGGACCAA CTCTTGAAGT TGTAGTCAGC AGAATGAAAA CTGTACAGTC TTTATCTCCT
    CCTTCAGAAA ATAGTACAGT TATTCCAATG AGATTTGTAG CTGTGTCTGC AACGATTCCA
    AATGCTGAGG ATATTGCAGA ATGGCTTTCA GATGGTGAAA GACCTGCAGT ATGTTTGAAA
    ATGGATGAGA AACATAGGCC AGTGAAACTT CAGAAAGTGG TCCTTGGATT CCCCTGCGGA
    AGTAACCAAA CTGAATTTAA GTTTGATTTA ACCCTCAACT ACAAAATTGC TGGTGTTATA
    CGAACATACT CTGATCAGAA ACCCACACTT GTGTTTTGTG CAACAAGGAA AGGTGTGCAA
    CAGGCTGCTT CTGTTCTAGT GAAAGATGCC AAATTTATTA TGGCTGTGGA ACAGAAACAG
    AGGTTACAGA AGTATGCATA TTCCATAAGA GATTCAAAAC TGAGAGATAT CTTAATACAT
    GGTGTTGCTT ATCATCATGC TGGTATGGAG CTGTCAGATA GAAAAGTAGT TGAAGGAGCT
    TTCACTGTTG GAGATCTACC AGTTCTTTTT ACTACCAGTA CTTTAGCTAT GGGAGTAAAT
    TTGCCTGCTC ACCTAGTAGT TATAAAATCT ACAATGCATT ATGCTGGAGG AATGTTTGAA
    GAATATAGTG AAACAGATAT TCTACAGATG ATTGGTAGAG CTGGTCGACC TCAATTTGAT
    ACTACAGCTA CTGCAGTTAT TATGACTCGG TTAAGTACAA AAGAGAAGTA TATTCAGATG
    TTAGCTTGTA GTGACACTGT AGAAAGCAGT TTACACAGAC ATCTTATCGA ACATTTAAAT
    GCAGAGATAG TGTTACATAC CATCACAGAT GTGAATATTG CTTTGGAATG GATACGATCA
    ACTTTGCTTT ATATCAGAGC CTTGAAAAAT CCATCTCATT ATGGTTTTGC ATCTGGATTG
    AACAAAGATG GAATTGAAGC AAAATTACAA GAATTATGTT TGAAGAATCT GCATGATTTA
    TCATCTCTGG ACTTGATAAG GATGGATGAA GATGTTAATT TCAAACCTAC TGAGGCAGGA
    AGATTGATGG CTTGGTATTA CATTACATTT GAGACAGTGA AGAAATTTTG TACTATCAGT
    GGAAAAGAGA CTTTATTAGA TCTGGTCACG ATGATATCCA GCTGCAAGGA ATTTGTAGAT
    GTGCAGTTAA GGATAAATGA AAAGAAAATA TTGAATACTT TGAACAAAGA TCCAAATAGA
    GTAACTATCA GATTTCCAAT GGAAGGAAGA ATTAAAACAA GAGAAATGAA AATAAATTGT
    CTTATACAGG CTCAACTAGG ATGCATTCCC ATACAAGACT TTGCTTTGAC ACAAGATATC
    TCGAAGATTT TTAGAAATGG CTCACGAATT ACAAGATGGT TGTCAGATTT TGTGGCTGTT
    CAAGAAAAGT TTGCTATATT ATTGAACAGT TTGATTTTAG CTAAATGTTT TAGATGCAAA
    CTTTGGGAAA ACTCTCTACA TGTATCCAAA CAGCTGGAAA AAATAGGTAT AACATTGTCA
    AATGCAATGG TAAATGCAGG TTTGACTTCC TTTAAAAAAA TAGAAGAAAC AGATGCAAGG
    GAACTTGAAC TGATTTTAAA TAGACACCCC CCTTTTGGAA CGCAAATAAA AGAAACAGTG
    ATGTATCTAC CAAAATATGA AGTTGAAGTG GAACAGATTG CAAGATACAG TGATACCATG
    GCAGAAATAT TAGTGACAAT TATATTAAGA AACTTTGAAC AGCTACAAGC TAGAAGAACA
    TCACCAGATT CTCACTATGT TACCTTAATC ATAGGTGATG CAGATAACCA GCTGGTTTTT
    AAGCACAAAA TTATGGATTC TGTTTTATTA AAAGCTGGAA ATTGGACTAA AAAGATTGAT
    GTGAGGAGAG CTTTTAAATC TGAAGATCTT AGCATAAATC TAATTAGTTC TGAGTATGTT
    GGACTTGATA TTCAGCAGAA ATTTACAGTC TTTTACTTAG GACCCAGGAG GCTTGGAAAT
    CAAATAATTA TGCAAAAAAA ATCAGAAACA GAGATTTCCC ATTCTAAACA TTCAAATAGA
    TCTACTATAG CAGGACCCAA TAAAGATTTA GGAATGACTG CTTGCAGAAA ACCTGGGAAC
    CGAGAATGTA ATCATCACTG TAAAAATAAA CACACATGTG GACATGACTG CTGTAAAACT
    GGAGTGGCAC AAAAGTCAGA AACAAAAGAG CCAACAATCT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCACTTCCT CCAGTTAAAC GTCTCAAGAT GCAAATGAAT
    AAATCTCGAA GTGTGGACCT TAAAGAATTT GGTTTTACTC CAAAACCTTC TGTTCCCAGT
    ATATCAAGGT CAGAGTGTTT AAACATACCT GGATTGTCTG TAATGGAGGA GAGGGATCAG
    CGTGATTTCT GTGTAAAAGT TCAACAAGAA CCGTCTGAAT ATGAAGACAA AGATTTCTCT
    AGATGGAACT ATTCTGCAAC TTCAAATGAG AAATCCCCTC AAACAGTGCT GTTCAACAGA
    CATCTTAATG CATCTGGAAA CCAAAATTTG ACAGATGGGG ATAAAAGTAA TTCTCCATCC
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAGAACTTAT TAGAAGTTAC TAGTTTTTCA GCCAGTACTG AGAAGACAAA AACATCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTACCAGG GGAAGTAAGC TATTGCTCCA AGAGCCAAAG
    AGCAAATTCC AAGGAGAAAT GACAAAAAGT TTTGTTTCAT CACATGGGAA GCCAGATACT
    TATTTATCAA ATTCTCCTGT ATTCGAGTTT GATACATCCT CCCTGACGAA ATTACCTCAA
    CAAGCTGGAA ATACAACTAT TGTCCATTCA CAAGAAAGAA AACAACAAAA TCTGTCACCA
    GAGATTGGAA AGCTATGCTT TACTCACTCT AAAAAAATAC CAAATTCTTC ACATTTTGTT
    AAGAAAGTGG ATTTCTTTAT TAGAAACAAT GAATGTAAAG AGGAAATTGA TTTCAGTAGG
    TATCATCCTG ATGATGAAGC TGATGAAATG AAGTCTTTCC TGGGAATATT TGATGACATT
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276215.1
    CDS39..4364
    Translation

    Target ORF information:

    RefSeq Version XM_022420507.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420507.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGACTCCTT GTTTTCTTTG GATACTTTGT TTTTTGAAAA ACCAGATGAA GCTGAAAAGT 
    CAGGAAGAAA GGCCAAAAAT GTTACCATCA AATTTAAAGA TGACTAATGA AAACACAAAT
    TATATTTCAC CAACACAAAA ATTCCAATTT GCCTTTTCCT CAAATAAATG TGAAACAGGT
    GATCTGAATT TAACAGAAGC AGGTAACCAT GACTTACCAC GTGTTCCTGG GAAGCTGACC
    TATGGTTCTC AGAAATACAA AAATCACATT GGCACCGAGA TAGCACCTGA GAAAAATGTT
    TCAGGTAATA CAAAATTAGT AAATTTTGCA GAAGATAAAG GAGAGAGCAC ATCAGCCTTC
    CGAAAAAGAT TATTTAAAAT ATCTGACAAT ATACATGAGG GTGTTTATTC TAATGACAGT
    ACAAACTTGG AGTCTTCTGT TGGCTCAGTG AAAATTGTCC CAACAGAAAT GAACAAAGGG
    GAATCATGGA AATGTAGCAA TAGTAAGCAG AAACATCAAT ATTCAGACAT TAATATGTTT
    ACAGCAAGCA ATGCTGTTTC AGCTTCTGAA ATCAGAGAAG ACATATTCAA AGGACCAACT
    TTTTCAGTTG CATCCCAACC TCATGAAGTT CAAGGGGTAA CAGAAAATGA TTTAGATTCT
    TTGAAGGCTG TCACAGAAAT CCCTGCAAAA TTTAGAAGTA TTTTCAAAGA ATTCCCATAT
    TTCAACTATA TACAGTCCAA AGCTTTTGAT GATCTTCTTT ACACAGATAG GAATTTTGTG
    ATTTGTGCTC CAACTGGTTC TGGGAAAACT GTGGTGTTTG AACTAGCTAT AACAAGATTG
    TTAATGGAAG TACCATTGCC ATGGTCAAAC ATAAAAATTG TTTATATGGC ACCAATAAAA
    GCCCTGTGTA GTCAGCGTTT CGATGATTGG AAAGAAAAAT TTGGACCAAT AGGATTGAAT
    TGTAAAGAAC TCACTGGAGA TACAGTAATG GATGACCTAT TTGAGATCCA ACATGCCCAT
    ATTATTATGA CAACTCCCGA AAAATGGGAT AGCATGACTA GGAAATGGAG AGACAACTCT
    TTGGTCCAGC TGGTTAGACT CTTTCTCATT GATGAGGTAC ATGTTGTAAA AGATGAAAAT
    CGGGGACCAA CTCTTGAAGT TGTAGTCAGC AGAATGAAAA CTGTACAGTC TTTATCTCCT
    CCTTCAGAAA ATAGTACAGT TATTCCAATG AGATTTGTAG CTGTGTCTGC AACGATTCCA
    AATGCTGAGG ATATTGCAGA ATGGCTTTCA GATGGTGAAA GACCTGCAGT ATGTTTGAAA
    ATGGATGAGA AACATAGGCC AGTGAAACTT CAGAAAGTGG TCCTTGGATT CCCCTGCGGA
    AGTAACCAAA CTGAATTTAA GTTTGATTTA ACCCTCAACT ACAAAATTGC TGGTGTTATA
    CGAACATACT CTGATCAGAA ACCCACACTT GTGTTTTGTG CAACAAGGAA AGGTGTGCAA
    CAGGCTGCTT CTGTTCTAGT GAAAGATGCC AAATTTATTA TGGCTGTGGA ACAGAAACAG
    AGGTTACAGA AGTATGCATA TTCCATAAGA GATTCAAAAC TGAGAGATAT CTTAATACAT
    GGTGTTGCTT ATCATCATGC TGGTATGGAG CTGTCAGATA GAAAAGTAGT TGAAGGAGCT
    TTCACTGTTG GAGATCTACC AGTTCTTTTT ACTACCAGTA CTTTAGCTAT GGGAGTAAAT
    TTGCCTGCTC ACCTAGTAGT TATAAAATCT ACAATGCATT ATGCTGGAGG AATGTTTGAA
    GAATATAGTG AAACAGATAT TCTACAGATG ATTGGTAGAG CTGGTCGACC TCAATTTGAT
    ACTACAGCTA CTGCAGTTAT TATGACTCGG TTAAGTACAA AAGAGAAGTA TATTCAGATG
    TTAGCTTGTA GTGACACTGT AGAAAGCAGT TTACACAGAC ATCTTATCGA ACATTTAAAT
    GCAGAGATAG TGTTACATAC CATCACAGAT GTGAATATTG CTTTGGAATG GATACGATCA
    ACTTTGCTTT ATATCAGAGC CTTGAAAAAT CCATCTCATT ATGGTTTTGC ATCTGGATTG
    AACAAAGATG GAATTGAAGC AAAATTACAA GAATTATGTT TGAAGAATCT GCATGATTTA
    TCATCTCTGG ACTTGATAAG GATGGATGAA GATGTTAATT TCAAACCTAC TGAGGCAGGA
    AGATTGATGG CTTGGTATTA CATTACATTT GAGACAGTGA AGAAATTTTG TACTATCAGT
    GGAAAAGAGA CTTTATTAGA TCTGGTCACG ATGATATCCA GCTGCAAGGA ATTTGTAGAT
    GTGCAGTTAA GGATAAATGA AAAGAAAATA TTGAATACTT TGAACAAAGA TCCAAATAGA
    GTAACTATCA GATTTCCAAT GGAAGGAAGA ATTAAAACAA GAGAAATGAA AATAAATTGT
    CTTATACAGG CTCAACTAGG ATGCATTCCC ATACAAGACT TTGCTTTGAC ACAAGATATC
    TCGAAGATTT TTAGAAATGG CTCACGAATT ACAAGATGGT TGTCAGATTT TGTGGCTGTT
    CAAGAAAAGT TTGCTATATT ATTGAACAGT TTGATTTTAG CTAAATGTTT TAGATGCAAA
    CTTTGGGAAA ACTCTCTACA TGTATCCAAA CAGCTGGAAA AAATAGGTAT AACATTGTCA
    AATGCAATGG TAAATGCAGG TTTGACTTCC TTTAAAAAAA TAGAAGAAAC AGATGCAAGG
    GAACTTGAAC TGATTTTAAA TAGACACCCC CCTTTTGGAA CGCAAATAAA AGAAACAGTG
    ATGTATCTAC CAAAATATGA AGTTGAAGTG GAACAGATTG CAAGATACAG TGATACCATG
    GCAGAAATAT TAGTGACAAT TATATTAAGA AACTTTGAAC AGCTACAAGC TAGAAGAACA
    TCACCAGATT CTCACTATGT TACCTTAATC ATAGGTGATG CAGATAACCA GCTGGTTTTT
    AAGCACAAAA TTATGGATTC TGTTTTATTA AAAGCTGGAA ATTGGACTAA AAAGATTGAT
    GTGAGGAGAG CTTTTAAATC TGAAGATCTT AGCATAAATC TAATTAGTTC TGAGTATGTT
    GGACTTGATA TTCAGCAGAA ATTTACAGTC TTTTACTTAG GACCCAGGAG GCTTGGAAAT
    CAAATAATTA TGCAAAAAAA ATCAGAAACA GAGATTTCCC ATTCTAAACA TTCAAATAGA
    TCTACTATAG CAGGACCCAA TAAAGATTTA GGAATGACTG CTTGCAGAAA ACCTGGGAAC
    CGAGAATGTA ATCATCACTG TAAAAATAAA CACACATGTG GACATGACTG CTGTAAAACT
    GGAGTGGCAC AAAAGTCAGA AACAAAAGAG CCAACAATCT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCACTTCCT CCAGTTAAAC GTCTCAAGAT GCAAATGAAT
    AAATCTCGAA GTGTGGACCT TAAAGAATTT GGTTTTACTC CAAAACCTTC TGTTCCCAGT
    ATATCAAGGT CAGAGTGTTT AAACATACCT GGATTGTCTG TAATGGAGGA GAGGGATCAG
    CGTGATTTCT GTGTAAAAGT TCAACAAGAA CCGTCTGAAT ATGAAGACAA AGATTTCTCT
    AGATGGAACT ATTCTGCAAC TTCAAATGAG AAATCCCCTC AAACAGTGCT GTTCAACAGA
    CATCTTAATG CATCTGGAAA CCAAAATTTG ACAGATGGGG ATAAAAGTAA TTCTCCATCC
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAGAACTTAT TAGAAGTTAC TAGTTTTTCA GCCAGTACTG AGAAGACAAA AACATCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTACCAGG GGAAGTAAGC TATTGCTCCA AGAGCCAAAG
    AGCAAATTCC AAGGAGAAAT GACAAAAAGT TTTGTTTCAT CACATGGGAA GCCAGATACT
    TATTTATCAA ATTCTCCTGT ATTCGAGTTT GATACATCCT CCCTGACGAA ATTACCTCAA
    CAAGCTGGAA ATACAACTAT TGTCCATTCA CAAGAAAGAA AACAACAAAA TCTGTCACCA
    GAGATTGGAA AGCTATGCTT TACTCACTCT AAAAAAATAC CAAATTCTTC ACATTTTGTT
    AAGAAAGTGG ATTTCTTTAT TAGAAACAAT GAATGTAAAG AGGAAATTGA TTTCAGTAGG
    TATCATCCTG ATGATGAAGC TGATGAAATG AAGTCTTTCC TGGGAATATT TGATGACATT
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289271
    Clone ID Related Accession (Same CDS sequence) XM_022420508.1
    Accession Version XM_022420508.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGTTTC AGCTTCTGAA ATCAGAGAAG ACATATTCAA AGGACCAACT TTTTCAGTTG 
    CATCCCAACC TCATGAAGTT CAAGCTTCTT TACACAGATA GGAATTTTGT GATTTGTGCT
    CCAACTGGTT CTGGGAAAAC TGTGGTGTTT GAACTAGCTA TAACAAGATT GTTAATGGAA
    GTACCATTGC CATGGTCAAA CATAAAAATT GTTTATATGG CACCAATAAA AGCCCTGTGT
    AGTCAGCGTT TCGATGATTG GAAAGAAAAA TTTGGACCAA TAGGATTGAA TTGTAAAGAA
    CTCACTGGAG ATACAGTAAT GGATGACCTA TTTGAGATCC AACATGCCCA TATTATTATG
    ACAACTCCCG AAAAATGGGA TAGCATGACT AGGAAATGGA GAGACAACTC TTTGGTCCAG
    CTGGTTAGAC TCTTTCTCAT TGATGAGGTA CATGTTGTAA AAGATGAAAA TCGGGGACCA
    ACTCTTGAAG TTGTAGTCAG CAGAATGAAA ACTGTACAGT CTTTATCTCC TCCTTCAGAA
    AATAGTACAG TTATTCCAAT GAGATTTGTA GCTGTGTCTG CAACGATTCC AAATGCTGAG
    GATATTGCAG AATGGCTTTC AGATGGTGAA AGACCTGCAG TATGTTTGAA AATGGATGAG
    AAACATAGGC CAGTGAAACT TCAGAAAGTG GTCCTTGGAT TCCCCTGCGG AAGTAACCAA
    ACTGAATTTA AGTTTGATTT AACCCTCAAC TACAAAATTG CTGGTGTTAT ACGAACATAC
    TCTGATCAGA AACCCACACT TGTGTTTTGT GCAACAAGGA AAGGTGTGCA ACAGGCTGCT
    TCTGTTCTAG TGAAAGATGC CAAATTTATT ATGGCTGTGG AACAGAAACA GAGGTTACAG
    AAGTATGCAT ATTCCATAAG AGATTCAAAA CTGAGAGATA TCTTAATACA TGGTGTTGCT
    TATCATCATG CTGGTATGGA GCTGTCAGAT AGAAAAGTAG TTGAAGGAGC TTTCACTGTT
    GGAGATCTAC CAGTTCTTTT TACTACCAGT ACTTTAGCTA TGGGAGTAAA TTTGCCTGCT
    CACCTAGTAG TTATAAAATC TACAATGCAT TATGCTGGAG GAATGTTTGA AGAATATAGT
    GAAACAGATA TTCTACAGAT GATTGGTAGA GCTGGTCGAC CTCAATTTGA TACTACAGCT
    ACTGCAGTTA TTATGACTCG GTTAAGTACA AAAGAGAAGT ATATTCAGAT GTTAGCTTGT
    AGTGACACTG TAGAAAGCAG TTTACACAGA CATCTTATCG AACATTTAAA TGCAGAGATA
    GTGTTACATA CCATCACAGA TGTGAATATT GCTTTGGAAT GGATACGATC AACTTTGCTT
    TATATCAGAG CCTTGAAAAA TCCATCTCAT TATGGTTTTG CATCTGGATT GAACAAAGAT
    GGAATTGAAG CAAAATTACA AGAATTATGT TTGAAGAATC TGCATGATTT ATCATCTCTG
    GACTTGATAA GGATGGATGA AGATGTTAAT TTCAAACCTA CTGAGGCAGG AAGATTGATG
    GCTTGGTATT ACATTACATT TGAGACAGTG AAGAAATTTT GTACTATCAG TGGAAAAGAG
    ACTTTATTAG ATCTGGTCAC GATGATATCC AGCTGCAAGG AATTTGTAGA TGTGCAGTTA
    AGGATAAATG AAAAGAAAAT ATTGAATACT TTGAACAAAG ATCCAAATAG AGTAACTATC
    AGATTTCCAA TGGAAGGAAG AATTAAAACA AGAGAAATGA AAATAAATTG TCTTATACAG
    GCTCAACTAG GATGCATTCC CATACAAGAC TTTGCTTTGA CACAAGATAT CTCGAAGATT
    TTTAGAAATG GCTCACGAAT TACAAGATGG TTGTCAGATT TTGTGGCTGT TCAAGAAAAG
    TTTGCTATAT TATTGAACAG TTTGATTTTA GCTAAATGTT TTAGATGCAA ACTTTGGGAA
    AACTCTCTAC ATGTATCCAA ACAGCTGGAA AAAATAGGTA TAACATTGTC AAATGCAATG
    GTAAATGCAG GTTTGACTTC CTTTAAAAAA ATAGAAGAAA CAGATGCAAG GGAACTTGAA
    CTGATTTTAA ATAGACACCC CCCTTTTGGA ACGCAAATAA AAGAAACAGT GATGTATCTA
    CCAAAATATG AAGTTGAAGT GGAACAGATT GCAAGATACA GTGATACCAT GGCAGAAATA
    TTAGTGACAA TTATATTAAG AAACTTTGAA CAGCTACAAG CTAGAAGAAC ATCACCAGAT
    TCTCACTATG TTACCTTAAT CATAGGTGAT GCAGATAACC AGCTGGTTTT TAAGCACAAA
    ATTATGGATT CTGTTTTATT AAAAGCTGGA AATTGGACTA AAAAGATTGA TGTGAGGAGA
    GCTTTTAAAT CTGAAGATCT TAGCATAAAT CTAATTAGTT CTGAGTATGT TGGACTTGAT
    ATTCAGCAGA AATTTACAGT CTTTTACTTA GGACCCAGGA GGCTTGGAAA TCAAATAATT
    ATGCAAAAAA AATCAGAAAC AGAGATTTCC CATTCTAAAC ATTCAAATAG ATCTACTATA
    GCAGGACCCA ATAAAGATTT AGGAATGACT GCTTGCAGAA AACCTGGGAA CCGAGAATGT
    AATCATCACT GTAAAAATAA ACACACATGT GGACATGACT GCTGTAAAAC TGGAGTGGCA
    CAAAAGTCAG AAACAAAAGA GCCAACAATC TCTTCATATT TATCTGATTT AAGAAACAGG
    AATGCTGTTT CATCACTTCC TCCAGTTAAA CGTCTCAAGA TGCAAATGAA TAAATCTCGA
    AGTGTGGACC TTAAAGAATT TGGTTTTACT CCAAAACCTT CTGTTCCCAG TATATCAAGG
    TCAGAGTGTT TAAACATACC TGGATTGTCT GTAATGGAGG AGAGGGATCA GCGTGATTTC
    TGTGTAAAAG TTCAACAAGA ACCGTCTGAA TATGAAGACA AAGATTTCTC TAGATGGAAC
    TATTCTGCAA CTTCAAATGA GAAATCCCCT CAAACAGTGC TGTTCAACAG ACATCTTAAT
    GCATCTGGAA ACCAAAATTT GACAGATGGG GATAAAAGTA ATTCTCCATC CTCAGAAGTT
    TTGAATGTGA ACTTTGAATT GGGAAATGAA GTTTGGGATG ATTTTGATGA TGAGAACTTA
    TTAGAAGTTA CTAGTTTTTC AGCCAGTACT GAGAAGACAA AAACATCAGG ATTTGGAAAC
    ACTTTGAGTT CAAGTACCAG GGGAAGTAAG CTATTGCTCC AAGAGCCAAA GAGCAAATTC
    CAAGGAGAAA TGACAAAAAG TTTTGTTTCA TCACATGGGA AGCCAGATAC TTATTTATCA
    AATTCTCCTG TATTCGAGTT TGATACATCC TCCCTGACGA AATTACCTCA ACAAGCTGGA
    AATACAACTA TTGTCCATTC ACAAGAAAGA AAACAACAAA ATCTGTCACC AGAGATTGGA
    AAGCTATGCT TTACTCACTC TAAAAAAATA CCAAATTCTT CACATTTTGT TAAGAAAGTG
    GATTTCTTTA TTAGAAACAA TGAATGTAAA GAGGAAATTG ATTTCAGTAG GTATCATCCT
    GATGATGAAG CTGATGAAAT GAAGTCTTTC CTGGGAATAT TTGATGACAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276216.1
    CDS143..3799
    Translation

    Target ORF information:

    RefSeq Version XM_022420508.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420508.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGTTTC AGCTTCTGAA ATCAGAGAAG ACATATTCAA AGGACCAACT TTTTCAGTTG 
    CATCCCAACC TCATGAAGTT CAAGCTTCTT TACACAGATA GGAATTTTGT GATTTGTGCT
    CCAACTGGTT CTGGGAAAAC TGTGGTGTTT GAACTAGCTA TAACAAGATT GTTAATGGAA
    GTACCATTGC CATGGTCAAA CATAAAAATT GTTTATATGG CACCAATAAA AGCCCTGTGT
    AGTCAGCGTT TCGATGATTG GAAAGAAAAA TTTGGACCAA TAGGATTGAA TTGTAAAGAA
    CTCACTGGAG ATACAGTAAT GGATGACCTA TTTGAGATCC AACATGCCCA TATTATTATG
    ACAACTCCCG AAAAATGGGA TAGCATGACT AGGAAATGGA GAGACAACTC TTTGGTCCAG
    CTGGTTAGAC TCTTTCTCAT TGATGAGGTA CATGTTGTAA AAGATGAAAA TCGGGGACCA
    ACTCTTGAAG TTGTAGTCAG CAGAATGAAA ACTGTACAGT CTTTATCTCC TCCTTCAGAA
    AATAGTACAG TTATTCCAAT GAGATTTGTA GCTGTGTCTG CAACGATTCC AAATGCTGAG
    GATATTGCAG AATGGCTTTC AGATGGTGAA AGACCTGCAG TATGTTTGAA AATGGATGAG
    AAACATAGGC CAGTGAAACT TCAGAAAGTG GTCCTTGGAT TCCCCTGCGG AAGTAACCAA
    ACTGAATTTA AGTTTGATTT AACCCTCAAC TACAAAATTG CTGGTGTTAT ACGAACATAC
    TCTGATCAGA AACCCACACT TGTGTTTTGT GCAACAAGGA AAGGTGTGCA ACAGGCTGCT
    TCTGTTCTAG TGAAAGATGC CAAATTTATT ATGGCTGTGG AACAGAAACA GAGGTTACAG
    AAGTATGCAT ATTCCATAAG AGATTCAAAA CTGAGAGATA TCTTAATACA TGGTGTTGCT
    TATCATCATG CTGGTATGGA GCTGTCAGAT AGAAAAGTAG TTGAAGGAGC TTTCACTGTT
    GGAGATCTAC CAGTTCTTTT TACTACCAGT ACTTTAGCTA TGGGAGTAAA TTTGCCTGCT
    CACCTAGTAG TTATAAAATC TACAATGCAT TATGCTGGAG GAATGTTTGA AGAATATAGT
    GAAACAGATA TTCTACAGAT GATTGGTAGA GCTGGTCGAC CTCAATTTGA TACTACAGCT
    ACTGCAGTTA TTATGACTCG GTTAAGTACA AAAGAGAAGT ATATTCAGAT GTTAGCTTGT
    AGTGACACTG TAGAAAGCAG TTTACACAGA CATCTTATCG AACATTTAAA TGCAGAGATA
    GTGTTACATA CCATCACAGA TGTGAATATT GCTTTGGAAT GGATACGATC AACTTTGCTT
    TATATCAGAG CCTTGAAAAA TCCATCTCAT TATGGTTTTG CATCTGGATT GAACAAAGAT
    GGAATTGAAG CAAAATTACA AGAATTATGT TTGAAGAATC TGCATGATTT ATCATCTCTG
    GACTTGATAA GGATGGATGA AGATGTTAAT TTCAAACCTA CTGAGGCAGG AAGATTGATG
    GCTTGGTATT ACATTACATT TGAGACAGTG AAGAAATTTT GTACTATCAG TGGAAAAGAG
    ACTTTATTAG ATCTGGTCAC GATGATATCC AGCTGCAAGG AATTTGTAGA TGTGCAGTTA
    AGGATAAATG AAAAGAAAAT ATTGAATACT TTGAACAAAG ATCCAAATAG AGTAACTATC
    AGATTTCCAA TGGAAGGAAG AATTAAAACA AGAGAAATGA AAATAAATTG TCTTATACAG
    GCTCAACTAG GATGCATTCC CATACAAGAC TTTGCTTTGA CACAAGATAT CTCGAAGATT
    TTTAGAAATG GCTCACGAAT TACAAGATGG TTGTCAGATT TTGTGGCTGT TCAAGAAAAG
    TTTGCTATAT TATTGAACAG TTTGATTTTA GCTAAATGTT TTAGATGCAA ACTTTGGGAA
    AACTCTCTAC ATGTATCCAA ACAGCTGGAA AAAATAGGTA TAACATTGTC AAATGCAATG
    GTAAATGCAG GTTTGACTTC CTTTAAAAAA ATAGAAGAAA CAGATGCAAG GGAACTTGAA
    CTGATTTTAA ATAGACACCC CCCTTTTGGA ACGCAAATAA AAGAAACAGT GATGTATCTA
    CCAAAATATG AAGTTGAAGT GGAACAGATT GCAAGATACA GTGATACCAT GGCAGAAATA
    TTAGTGACAA TTATATTAAG AAACTTTGAA CAGCTACAAG CTAGAAGAAC ATCACCAGAT
    TCTCACTATG TTACCTTAAT CATAGGTGAT GCAGATAACC AGCTGGTTTT TAAGCACAAA
    ATTATGGATT CTGTTTTATT AAAAGCTGGA AATTGGACTA AAAAGATTGA TGTGAGGAGA
    GCTTTTAAAT CTGAAGATCT TAGCATAAAT CTAATTAGTT CTGAGTATGT TGGACTTGAT
    ATTCAGCAGA AATTTACAGT CTTTTACTTA GGACCCAGGA GGCTTGGAAA TCAAATAATT
    ATGCAAAAAA AATCAGAAAC AGAGATTTCC CATTCTAAAC ATTCAAATAG ATCTACTATA
    GCAGGACCCA ATAAAGATTT AGGAATGACT GCTTGCAGAA AACCTGGGAA CCGAGAATGT
    AATCATCACT GTAAAAATAA ACACACATGT GGACATGACT GCTGTAAAAC TGGAGTGGCA
    CAAAAGTCAG AAACAAAAGA GCCAACAATC TCTTCATATT TATCTGATTT AAGAAACAGG
    AATGCTGTTT CATCACTTCC TCCAGTTAAA CGTCTCAAGA TGCAAATGAA TAAATCTCGA
    AGTGTGGACC TTAAAGAATT TGGTTTTACT CCAAAACCTT CTGTTCCCAG TATATCAAGG
    TCAGAGTGTT TAAACATACC TGGATTGTCT GTAATGGAGG AGAGGGATCA GCGTGATTTC
    TGTGTAAAAG TTCAACAAGA ACCGTCTGAA TATGAAGACA AAGATTTCTC TAGATGGAAC
    TATTCTGCAA CTTCAAATGA GAAATCCCCT CAAACAGTGC TGTTCAACAG ACATCTTAAT
    GCATCTGGAA ACCAAAATTT GACAGATGGG GATAAAAGTA ATTCTCCATC CTCAGAAGTT
    TTGAATGTGA ACTTTGAATT GGGAAATGAA GTTTGGGATG ATTTTGATGA TGAGAACTTA
    TTAGAAGTTA CTAGTTTTTC AGCCAGTACT GAGAAGACAA AAACATCAGG ATTTGGAAAC
    ACTTTGAGTT CAAGTACCAG GGGAAGTAAG CTATTGCTCC AAGAGCCAAA GAGCAAATTC
    CAAGGAGAAA TGACAAAAAG TTTTGTTTCA TCACATGGGA AGCCAGATAC TTATTTATCA
    AATTCTCCTG TATTCGAGTT TGATACATCC TCCCTGACGA AATTACCTCA ACAAGCTGGA
    AATACAACTA TTGTCCATTC ACAAGAAAGA AAACAACAAA ATCTGTCACC AGAGATTGGA
    AAGCTATGCT TTACTCACTC TAAAAAAATA CCAAATTCTT CACATTTTGT TAAGAAAGTG
    GATTTCTTTA TTAGAAACAA TGAATGTAAA GAGGAAATTG ATTTCAGTAG GTATCATCCT
    GATGATGAAG CTGATGAAAT GAAGTCTTTC CTGGGAATAT TTGATGACAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37267
    Clone ID Related Accession (Same CDS sequence) XM_014114844.1
    Accession Version XM_014114844.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GGAATGACTG CTTGCAGAAA ACCTGGGAAC
    CGAGAATGTA ATCATCACTG TAAAAATAAA CACACATGTG GACATGACTG CTGTAAAACT
    GGAGTGGCAC AAAAGTCAGA AACAAAAGAG CCAACAATCT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCACTTCCT CCAGTTAAAC GTCTCAAGAT GCAAATGAAT
    AAATCTCGAA GTGTGGACCT TAAAGAATTT GGTTTTACTC CAAAACCTTC TGTTCCCAGT
    ATATCAAGGT CAGAGTGTTT AAACATACCT GGATTGTCTG TAATGGAGGA GAGGGATCAG
    CGTGATTTCT GTGTAAAAGT TCAACAAGAA CCGTCTGAAT ATGAAGACAA AGATTTCTCT
    AGATGGAACT ATTCTGCAAC TTCAAATGAG AAATCCCCTC AAACAGTGCT GTTCAACAGA
    CATCTTAATG CATCTGGAAA CCAAAATTTG ACAGATGGGG ATAAAAGTAA TTCTCCATCC
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAGAACTTAT TAGAAGTTAC TAGTTTTTCA GCCAGTACTG AGAAGACAAA AACATCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTACCAGG GGAAGTAAGC TATTGCTCCA AGAGCCAAAG
    AGCAAATTCC AAGGAGAAAT GACAAAAAGT TTTGTTTCAT CACATGGGAA GCCAGATACT
    TATTTATCAA ATTCTCCTGT ATTCGAGTTT GATACATCCT CCCTGACGAA ATTACCTCAA
    CAAGCTGGAA ATACAACTAT TGTCCATTCA CAAGAAAGAA AACAACAAAA TCTGTCACCA
    GAGATTGGAA AGCTATGCTT TACTCACTCT AAAAAAATAC CAAATTCTTC ACATTTTGTT
    AAGAAAGTGG ATTTCTTTAT TAGAAACAAT GAATGTAAAG AGGAAATTGA TTTCAGCATG
    TCAATTCCTG GCTGCTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970319.1
    CDS94..4494
    Translation

    Target ORF information:

    RefSeq Version XM_014114844.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014114844.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GGAATGACTG CTTGCAGAAA ACCTGGGAAC
    CGAGAATGTA ATCATCACTG TAAAAATAAA CACACATGTG GACATGACTG CTGTAAAACT
    GGAGTGGCAC AAAAGTCAGA AACAAAAGAG CCAACAATCT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCACTTCCT CCAGTTAAAC GTCTCAAGAT GCAAATGAAT
    AAATCTCGAA GTGTGGACCT TAAAGAATTT GGTTTTACTC CAAAACCTTC TGTTCCCAGT
    ATATCAAGGT CAGAGTGTTT AAACATACCT GGATTGTCTG TAATGGAGGA GAGGGATCAG
    CGTGATTTCT GTGTAAAAGT TCAACAAGAA CCGTCTGAAT ATGAAGACAA AGATTTCTCT
    AGATGGAACT ATTCTGCAAC TTCAAATGAG AAATCCCCTC AAACAGTGCT GTTCAACAGA
    CATCTTAATG CATCTGGAAA CCAAAATTTG ACAGATGGGG ATAAAAGTAA TTCTCCATCC
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAGAACTTAT TAGAAGTTAC TAGTTTTTCA GCCAGTACTG AGAAGACAAA AACATCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTACCAGG GGAAGTAAGC TATTGCTCCA AGAGCCAAAG
    AGCAAATTCC AAGGAGAAAT GACAAAAAGT TTTGTTTCAT CACATGGGAA GCCAGATACT
    TATTTATCAA ATTCTCCTGT ATTCGAGTTT GATACATCCT CCCTGACGAA ATTACCTCAA
    CAAGCTGGAA ATACAACTAT TGTCCATTCA CAAGAAAGAA AACAACAAAA TCTGTCACCA
    GAGATTGGAA AGCTATGCTT TACTCACTCT AAAAAAATAC CAAATTCTTC ACATTTTGTT
    AAGAAAGTGG ATTTCTTTAT TAGAAACAAT GAATGTAAAG AGGAAATTGA TTTCAGCATG
    TCAATTCCTG GCTGCTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08612
    Clone ID Related Accession (Same CDS sequence) XM_005621940.1
    Accession Version XM_005621940.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGTTTC AGCTTCTGAA ATCAGAGAAG ACATATTCAA AGGACCAACT TTTTCAGTTG 
    CATCCCAACC TCATGAAGTT CAAGCTTCTT TACACAGATA GGAATTTTGT GATTTGTGCT
    CCAACTGGTT CTGGGAAAAC TGTGGTGTTT GAACTAGCTA TAACAAGATT GTTAATGGAA
    GTACCATTGC CATGGTCAAA CATAAAAATT GTTTATATGG CACCAATAAA AGCCCTGTGT
    AGTCAGCGTT TCGATGATTG GAAAGAAAAA TTTGGACCAA TAGGATTGAA TTGTAAAGAA
    CTCACTGGAG ATACAGTAAT GGATGACCTA TTTGAGATCC AACATGCCCA TATTATTATG
    ACAACTCCCG AAAAATGGGA TAGCATGACT AGGAAATGGA GAGACAACTC TTTGGTCCAG
    CTGGTTAGAC TCTTTCTCAT TGATGAGGTA CATGTTGTAA AAGATGAAAA TCGGGGACCA
    ACTCTTGAAG TTGTAGTCAG CAGAATGAAA ACTGTACAGT CTTTATCTCC TCCTTCAGAA
    AATAGTACAG TTATTCCAAT GAGATTTGTA GCTGTGTCTG CAACGATTCC AAATGCTGAG
    GATATTGCAG AATGGCTTTC AGATGGTGAA AGACCTGCAG TATGTTTGAA AATGGATGAG
    AAACATAGGC CAGTGAAACT TCAGAAAGTG GTCCTTGGAT TCCCCTGCGG AAGTAACCAA
    ACTGAATTTA AGTTTGATTT AACCCTCAAC TACAAAATTG CTGGTGTTAT ACGAACATAC
    TCTGATCAGA AACCCACACT TGTGTTTTGT GCAACAAGGA AAGGTGTGCA ACAGGCTGCT
    TCTGTTCTAG TGAAAGATGC CAAATTTATT ATGGCTGTGG AACAGAAACA GAGGTTACAG
    AAGTATGCAT ATTCCATAAG AGATTCAAAA CTGAGAGATA TCTTAATACA TGGTGTTGCT
    TATCATCATG CTGGTATGGA GCTGTCAGAT AGAAAAGTAG TTGAAGGAGC TTTCACTGTT
    GGAGATCTAC CAGTTCTTTT TACTACCAGT ACTTTAGCTA TGGGAGTAAA TTTGCCTGCT
    CACCTAGTAG TTATAAAATC TACAATGCAT TATGCTGGAG GAATGTTTGA AGAATATAGT
    GAAACAGATA TTCTACAGAT GATTGGTAGA GCTGGTCGAC CTCAATTTGA TACTACAGCT
    ACTGCAGTTA TTATGACTCG GTTAAGTACA AAAGAGAAGT ATATTCAGAT GTTAGCTTGT
    AGTGACACTG TAGAAAGCAG TTTACACAGA CATCTTATCG AACATTTAAA TGCAGAGATA
    GTGTTACATA CCATCACAGA TGTGAATATT GCTTTGGAAT GGATACGATC AACTTTGCTT
    TATATCAGAG CCTTGAAAAA TCCATCTCAT TATGGTTTTG CATCTGGATT GAACAAAGAT
    GGAATTGAAG CAAAATTACA AGAATTATGT TTGAAGAATC TGCATGATTT ATCATCTCTG
    GACTTGATAA GGATGGATGA AGATGTTAAT TTCAAACCTA CTGAGGCAGG AAGATTGATG
    GCTTGGTATT ACATTACATT TGAGACAGTG AAGAAATTTT GTACTATCAG TGGAAAAGAG
    ACTTTATTAG ATCTGGTCAC GATGATATCC AGCTGCAAGG AATTTGTAGA TGTGCAGTTA
    AGGATAAATG AAAAGAAAAT ATTGAATACT TTGAACAAAG ATCCAAATAG AGTAACTATC
    AGATTTCCAA TGGAAGGAAG AATTAAAACA AGAGAAATGA AAATAAATTG TCTTATACAG
    GCTCAACTAG GATGCATTCC CATACAAGAC TTTGCTTTGA CACAAGATAT CTCGAAGATT
    TTTAGAAATG GCTCACGAAT TACAAGATGG TTGTCAGATT TTGTGGCTGT TCAAGAAAAG
    TTTGCTATAT TATTGAACAG TTTGATTTTA GCTAAATGTT TTAGATGCAA ACTTTGGGAA
    AACTCTCTAC ATGTATCCAA ACAGCTGGAA AAAATAGGTA TAACATTGTC AAATGCAATG
    GTAAATGCAG GTTTGACTTC CTTTAAAAAA ATAGAAGAAA CAGATGCAAG GGAACTTGAA
    CTGATTTTAA ATAGACACCC CCCTTTTGGA ACGCAAATAA AAGAAACAGT GATGTATCTA
    CCAAAATATG AAGTTGAAGT GGAACAGATT GCAAGATACA GTGATACCAT GGCAGAAATA
    TTAGTGACAA TTATATTAAG AAACTTTGAA CAGCTACAAG CTAGAAGAAC ATCACCAGAT
    TCTCACTATG TTACCTTAAT CATAGGTGAT GCAGATAACC AGCTGGTTTT TAAGCACAAA
    ATTATGGATT CTGTTTTATT AAAAGCTGGA AATTGGACTA AAAAGATTGA TGTGAGGAGA
    GCTTTTAAAT CTGAAGATCT TAGCATAAAT CTAATTAGTT CTGAGTATGT TGGACTTGAT
    ATTCAGCAGA AATTTACAGT CTTTTACTTA GGACCCAGGA GGCTTGGAAA TCAAATAATT
    ATGCAAAAAA AATCAGAAAC AGAGATTTCC CATTCTAAAC ATTCAAATAG ATCTACTATA
    GCAGGACCCA ATAAAGGAAT GACTGCTTGC AGAAAACCTG GGAACCGAGA ATGTAATCAT
    CACTGTAAAA ATAAACACAC ATGTGGACAT GACTGCTGTA AAACTGGAGT GGCACAAAAG
    TCAGAAACAA AAGAGCCAAC AATCTCTTCA TATTTATCTG ATTTAAGAAA CAGGAATGCT
    GTTTCATCAC TTCCTCCAGT TAAACGTCTC AAGATGCAAA TGAATAAATC TCGAAGTGTG
    GACCTTAAAG AATTTGGTTT TACTCCAAAA CCTTCTGTTC CCAGTATATC AAGGTCAGAG
    TGTTTAAACA TACCTGGATT GTCTGTAATG GAGGAGAGGG ATCAGCGTGA TTTCTGTGTA
    AAAGTTCAAC AAGAACCGTC TGAATATGAA GACAAAGATT TCTCTAGATG GAACTATTCT
    GCAACTTCAA ATGAGAAATC CCCTCAAACA GTGCTGTTCA ACAGACATCT TAATGCATCT
    GGAAACCAAA ATTTGACAGA TGGGGATAAA AGTAATTCTC CATCCTCAGA AGTTTTGAAT
    GTGAACTTTG AATTGGGAAA TGAAGTTTGG GATGATTTTG ATGATGAGAA CTTATTAGAA
    GTTACTAGTT TTTCAGCCAG TACTGAGAAG ACAAAAACAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTATTG CTCCAAGAGC CAAAGAGCAA ATTCCAAGGA
    GAAATGACAA AAAGTTTTGT TTCATCACAT GGGAAGCCAG ATACTTATTT ATCAAATTCT
    CCTGTATTCG AGTTTGATAC ATCCTCCCTG ACGAAATTAC CTCAACAAGC TGGAAATACA
    ACTATTGTCC ATTCACAAGA AAGAAAACAA CAAAATCTGT CACCAGAGAT TGGAAAGCTA
    TGCTTTACTC ACTCTAAAAA AATACCAAAT TCTTCACATT TTGTTAAGAA AGTGGATTTC
    TTTATTAGAA ACAATGAATG TAAAGAGGAA ATTGATTTCA GTAGGTATCA TCCTGATGAT
    GAAGCTGATG AAATGAAGTC TTTCCTGGGA ATATTTGATG ACATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621997.1
    CDS98..3748
    Translation

    Target ORF information:

    RefSeq Version XM_005621940.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621940.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGTTTC AGCTTCTGAA ATCAGAGAAG ACATATTCAA AGGACCAACT TTTTCAGTTG 
    CATCCCAACC TCATGAAGTT CAAGCTTCTT TACACAGATA GGAATTTTGT GATTTGTGCT
    CCAACTGGTT CTGGGAAAAC TGTGGTGTTT GAACTAGCTA TAACAAGATT GTTAATGGAA
    GTACCATTGC CATGGTCAAA CATAAAAATT GTTTATATGG CACCAATAAA AGCCCTGTGT
    AGTCAGCGTT TCGATGATTG GAAAGAAAAA TTTGGACCAA TAGGATTGAA TTGTAAAGAA
    CTCACTGGAG ATACAGTAAT GGATGACCTA TTTGAGATCC AACATGCCCA TATTATTATG
    ACAACTCCCG AAAAATGGGA TAGCATGACT AGGAAATGGA GAGACAACTC TTTGGTCCAG
    CTGGTTAGAC TCTTTCTCAT TGATGAGGTA CATGTTGTAA AAGATGAAAA TCGGGGACCA
    ACTCTTGAAG TTGTAGTCAG CAGAATGAAA ACTGTACAGT CTTTATCTCC TCCTTCAGAA
    AATAGTACAG TTATTCCAAT GAGATTTGTA GCTGTGTCTG CAACGATTCC AAATGCTGAG
    GATATTGCAG AATGGCTTTC AGATGGTGAA AGACCTGCAG TATGTTTGAA AATGGATGAG
    AAACATAGGC CAGTGAAACT TCAGAAAGTG GTCCTTGGAT TCCCCTGCGG AAGTAACCAA
    ACTGAATTTA AGTTTGATTT AACCCTCAAC TACAAAATTG CTGGTGTTAT ACGAACATAC
    TCTGATCAGA AACCCACACT TGTGTTTTGT GCAACAAGGA AAGGTGTGCA ACAGGCTGCT
    TCTGTTCTAG TGAAAGATGC CAAATTTATT ATGGCTGTGG AACAGAAACA GAGGTTACAG
    AAGTATGCAT ATTCCATAAG AGATTCAAAA CTGAGAGATA TCTTAATACA TGGTGTTGCT
    TATCATCATG CTGGTATGGA GCTGTCAGAT AGAAAAGTAG TTGAAGGAGC TTTCACTGTT
    GGAGATCTAC CAGTTCTTTT TACTACCAGT ACTTTAGCTA TGGGAGTAAA TTTGCCTGCT
    CACCTAGTAG TTATAAAATC TACAATGCAT TATGCTGGAG GAATGTTTGA AGAATATAGT
    GAAACAGATA TTCTACAGAT GATTGGTAGA GCTGGTCGAC CTCAATTTGA TACTACAGCT
    ACTGCAGTTA TTATGACTCG GTTAAGTACA AAAGAGAAGT ATATTCAGAT GTTAGCTTGT
    AGTGACACTG TAGAAAGCAG TTTACACAGA CATCTTATCG AACATTTAAA TGCAGAGATA
    GTGTTACATA CCATCACAGA TGTGAATATT GCTTTGGAAT GGATACGATC AACTTTGCTT
    TATATCAGAG CCTTGAAAAA TCCATCTCAT TATGGTTTTG CATCTGGATT GAACAAAGAT
    GGAATTGAAG CAAAATTACA AGAATTATGT TTGAAGAATC TGCATGATTT ATCATCTCTG
    GACTTGATAA GGATGGATGA AGATGTTAAT TTCAAACCTA CTGAGGCAGG AAGATTGATG
    GCTTGGTATT ACATTACATT TGAGACAGTG AAGAAATTTT GTACTATCAG TGGAAAAGAG
    ACTTTATTAG ATCTGGTCAC GATGATATCC AGCTGCAAGG AATTTGTAGA TGTGCAGTTA
    AGGATAAATG AAAAGAAAAT ATTGAATACT TTGAACAAAG ATCCAAATAG AGTAACTATC
    AGATTTCCAA TGGAAGGAAG AATTAAAACA AGAGAAATGA AAATAAATTG TCTTATACAG
    GCTCAACTAG GATGCATTCC CATACAAGAC TTTGCTTTGA CACAAGATAT CTCGAAGATT
    TTTAGAAATG GCTCACGAAT TACAAGATGG TTGTCAGATT TTGTGGCTGT TCAAGAAAAG
    TTTGCTATAT TATTGAACAG TTTGATTTTA GCTAAATGTT TTAGATGCAA ACTTTGGGAA
    AACTCTCTAC ATGTATCCAA ACAGCTGGAA AAAATAGGTA TAACATTGTC AAATGCAATG
    GTAAATGCAG GTTTGACTTC CTTTAAAAAA ATAGAAGAAA CAGATGCAAG GGAACTTGAA
    CTGATTTTAA ATAGACACCC CCCTTTTGGA ACGCAAATAA AAGAAACAGT GATGTATCTA
    CCAAAATATG AAGTTGAAGT GGAACAGATT GCAAGATACA GTGATACCAT GGCAGAAATA
    TTAGTGACAA TTATATTAAG AAACTTTGAA CAGCTACAAG CTAGAAGAAC ATCACCAGAT
    TCTCACTATG TTACCTTAAT CATAGGTGAT GCAGATAACC AGCTGGTTTT TAAGCACAAA
    ATTATGGATT CTGTTTTATT AAAAGCTGGA AATTGGACTA AAAAGATTGA TGTGAGGAGA
    GCTTTTAAAT CTGAAGATCT TAGCATAAAT CTAATTAGTT CTGAGTATGT TGGACTTGAT
    ATTCAGCAGA AATTTACAGT CTTTTACTTA GGACCCAGGA GGCTTGGAAA TCAAATAATT
    ATGCAAAAAA AATCAGAAAC AGAGATTTCC CATTCTAAAC ATTCAAATAG ATCTACTATA
    GCAGGACCCA ATAAAGGAAT GACTGCTTGC AGAAAACCTG GGAACCGAGA ATGTAATCAT
    CACTGTAAAA ATAAACACAC ATGTGGACAT GACTGCTGTA AAACTGGAGT GGCACAAAAG
    TCAGAAACAA AAGAGCCAAC AATCTCTTCA TATTTATCTG ATTTAAGAAA CAGGAATGCT
    GTTTCATCAC TTCCTCCAGT TAAACGTCTC AAGATGCAAA TGAATAAATC TCGAAGTGTG
    GACCTTAAAG AATTTGGTTT TACTCCAAAA CCTTCTGTTC CCAGTATATC AAGGTCAGAG
    TGTTTAAACA TACCTGGATT GTCTGTAATG GAGGAGAGGG ATCAGCGTGA TTTCTGTGTA
    AAAGTTCAAC AAGAACCGTC TGAATATGAA GACAAAGATT TCTCTAGATG GAACTATTCT
    GCAACTTCAA ATGAGAAATC CCCTCAAACA GTGCTGTTCA ACAGACATCT TAATGCATCT
    GGAAACCAAA ATTTGACAGA TGGGGATAAA AGTAATTCTC CATCCTCAGA AGTTTTGAAT
    GTGAACTTTG AATTGGGAAA TGAAGTTTGG GATGATTTTG ATGATGAGAA CTTATTAGAA
    GTTACTAGTT TTTCAGCCAG TACTGAGAAG ACAAAAACAT CAGGATTTGG AAACACTTTG
    AGTTCAAGTA CCAGGGGAAG TAAGCTATTG CTCCAAGAGC CAAAGAGCAA ATTCCAAGGA
    GAAATGACAA AAAGTTTTGT TTCATCACAT GGGAAGCCAG ATACTTATTT ATCAAATTCT
    CCTGTATTCG AGTTTGATAC ATCCTCCCTG ACGAAATTAC CTCAACAAGC TGGAAATACA
    ACTATTGTCC ATTCACAAGA AAGAAAACAA CAAAATCTGT CACCAGAGAT TGGAAAGCTA
    TGCTTTACTC ACTCTAAAAA AATACCAAAT TCTTCACATT TTGTTAAGAA AGTGGATTTC
    TTTATTAGAA ACAATGAATG TAAAGAGGAA ATTGATTTCA GTAGGTATCA TCCTGATGAT
    GAAGCTGATG AAATGAAGTC TTTCCTGGGA ATATTTGATG ACATTTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08610
    Clone ID Related Accession (Same CDS sequence) XM_005621938.2 , XM_005621938.1 , XM_005621939.2
    Accession Version XM_005621938.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GGAATGACTG CTTGCAGAAA ACCTGGGAAC
    CGAGAATGTA ATCATCACTG TAAAAATAAA CACACATGTG GACATGACTG CTGTAAAACT
    GGAGTGGCAC AAAAGTCAGA AACAAAAGAG CCAACAATCT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCACTTCCT CCAGTTAAAC GTCTCAAGAT GCAAATGAAT
    AAATCTCGAA GTGTGGACCT TAAAGAATTT GGTTTTACTC CAAAACCTTC TGTTCCCAGT
    ATATCAAGGT CAGAGTGTTT AAACATACCT GGATTGTCTG TAATGGAGGA GAGGGATCAG
    CGTGATTTCT GTGTAAAAGT TCAACAAGAA CCGTCTGAAT ATGAAGACAA AGATTTCTCT
    AGATGGAACT ATTCTGCAAC TTCAAATGAG AAATCCCCTC AAACAGTGCT GTTCAACAGA
    CATCTTAATG CATCTGGAAA CCAAAATTTG ACAGATGGGG ATAAAAGTAA TTCTCCATCC
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAGAACTTAT TAGAAGTTAC TAGTTTTTCA GCCAGTACTG AGAAGACAAA AACATCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTACCAGG GGAAGTAAGC TATTGCTCCA AGAGCCAAAG
    AGCAAATTCC AAGGAGAAAT GACAAAAAGT TTTGTTTCAT CACATGGGAA GCCAGATACT
    TATTTATCAA ATTCTCCTGT ATTCGAGTTT GATACATCCT CCCTGACGAA ATTACCTCAA
    CAAGCTGGAA ATACAACTAT TGTCCATTCA CAAGAAAGAA AACAACAAAA TCTGTCACCA
    GAGATTGGAA AGCTATGCTT TACTCACTCT AAAAAAATAC CAAATTCTTC ACATTTTGTT
    AAGAAAGTGG ATTTCTTTAT TAGAAACAAT GAATGTAAAG AGGAAATTGA TTTCAGTAGG
    TATCATCCTG ATGATGAAGC TGATGAAATG AAGTCTTTCC TGGGAATATT TGATGACATT
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621995.1
    CDS148..4593
    Translation

    Target ORF information:

    RefSeq Version XM_005621938.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621938.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GGAATGACTG CTTGCAGAAA ACCTGGGAAC
    CGAGAATGTA ATCATCACTG TAAAAATAAA CACACATGTG GACATGACTG CTGTAAAACT
    GGAGTGGCAC AAAAGTCAGA AACAAAAGAG CCAACAATCT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCACTTCCT CCAGTTAAAC GTCTCAAGAT GCAAATGAAT
    AAATCTCGAA GTGTGGACCT TAAAGAATTT GGTTTTACTC CAAAACCTTC TGTTCCCAGT
    ATATCAAGGT CAGAGTGTTT AAACATACCT GGATTGTCTG TAATGGAGGA GAGGGATCAG
    CGTGATTTCT GTGTAAAAGT TCAACAAGAA CCGTCTGAAT ATGAAGACAA AGATTTCTCT
    AGATGGAACT ATTCTGCAAC TTCAAATGAG AAATCCCCTC AAACAGTGCT GTTCAACAGA
    CATCTTAATG CATCTGGAAA CCAAAATTTG ACAGATGGGG ATAAAAGTAA TTCTCCATCC
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAGAACTTAT TAGAAGTTAC TAGTTTTTCA GCCAGTACTG AGAAGACAAA AACATCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTACCAGG GGAAGTAAGC TATTGCTCCA AGAGCCAAAG
    AGCAAATTCC AAGGAGAAAT GACAAAAAGT TTTGTTTCAT CACATGGGAA GCCAGATACT
    TATTTATCAA ATTCTCCTGT ATTCGAGTTT GATACATCCT CCCTGACGAA ATTACCTCAA
    CAAGCTGGAA ATACAACTAT TGTCCATTCA CAAGAAAGAA AACAACAAAA TCTGTCACCA
    GAGATTGGAA AGCTATGCTT TACTCACTCT AAAAAAATAC CAAATTCTTC ACATTTTGTT
    AAGAAAGTGG ATTTCTTTAT TAGAAACAAT GAATGTAAAG AGGAAATTGA TTTCAGTAGG
    TATCATCCTG ATGATGAAGC TGATGAAATG AAGTCTTTCC TGGGAATATT TGATGACATT
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08610
    Clone ID Related Accession (Same CDS sequence) XM_005621938.2 , XM_005621938.1 , XM_005621939.2
    Accession Version XM_005621938.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GGAATGACTG CTTGCAGAAA ACCTGGGAAC
    CGAGAATGTA ATCATCACTG TAAAAATAAA CACACATGTG GACATGACTG CTGTAAAACT
    GGAGTGGCAC AAAAGTCAGA AACAAAAGAG CCAACAATCT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCACTTCCT CCAGTTAAAC GTCTCAAGAT GCAAATGAAT
    AAATCTCGAA GTGTGGACCT TAAAGAATTT GGTTTTACTC CAAAACCTTC TGTTCCCAGT
    ATATCAAGGT CAGAGTGTTT AAACATACCT GGATTGTCTG TAATGGAGGA GAGGGATCAG
    CGTGATTTCT GTGTAAAAGT TCAACAAGAA CCGTCTGAAT ATGAAGACAA AGATTTCTCT
    AGATGGAACT ATTCTGCAAC TTCAAATGAG AAATCCCCTC AAACAGTGCT GTTCAACAGA
    CATCTTAATG CATCTGGAAA CCAAAATTTG ACAGATGGGG ATAAAAGTAA TTCTCCATCC
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAGAACTTAT TAGAAGTTAC TAGTTTTTCA GCCAGTACTG AGAAGACAAA AACATCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTACCAGG GGAAGTAAGC TATTGCTCCA AGAGCCAAAG
    AGCAAATTCC AAGGAGAAAT GACAAAAAGT TTTGTTTCAT CACATGGGAA GCCAGATACT
    TATTTATCAA ATTCTCCTGT ATTCGAGTTT GATACATCCT CCCTGACGAA ATTACCTCAA
    CAAGCTGGAA ATACAACTAT TGTCCATTCA CAAGAAAGAA AACAACAAAA TCTGTCACCA
    GAGATTGGAA AGCTATGCTT TACTCACTCT AAAAAAATAC CAAATTCTTC ACATTTTGTT
    AAGAAAGTGG ATTTCTTTAT TAGAAACAAT GAATGTAAAG AGGAAATTGA TTTCAGTAGG
    TATCATCCTG ATGATGAAGC TGATGAAATG AAGTCTTTCC TGGGAATATT TGATGACATT
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621995.1
    CDS94..4539
    Translation

    Target ORF information:

    RefSeq Version XM_005621938.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621938.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GGAATGACTG CTTGCAGAAA ACCTGGGAAC
    CGAGAATGTA ATCATCACTG TAAAAATAAA CACACATGTG GACATGACTG CTGTAAAACT
    GGAGTGGCAC AAAAGTCAGA AACAAAAGAG CCAACAATCT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCACTTCCT CCAGTTAAAC GTCTCAAGAT GCAAATGAAT
    AAATCTCGAA GTGTGGACCT TAAAGAATTT GGTTTTACTC CAAAACCTTC TGTTCCCAGT
    ATATCAAGGT CAGAGTGTTT AAACATACCT GGATTGTCTG TAATGGAGGA GAGGGATCAG
    CGTGATTTCT GTGTAAAAGT TCAACAAGAA CCGTCTGAAT ATGAAGACAA AGATTTCTCT
    AGATGGAACT ATTCTGCAAC TTCAAATGAG AAATCCCCTC AAACAGTGCT GTTCAACAGA
    CATCTTAATG CATCTGGAAA CCAAAATTTG ACAGATGGGG ATAAAAGTAA TTCTCCATCC
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAGAACTTAT TAGAAGTTAC TAGTTTTTCA GCCAGTACTG AGAAGACAAA AACATCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTACCAGG GGAAGTAAGC TATTGCTCCA AGAGCCAAAG
    AGCAAATTCC AAGGAGAAAT GACAAAAAGT TTTGTTTCAT CACATGGGAA GCCAGATACT
    TATTTATCAA ATTCTCCTGT ATTCGAGTTT GATACATCCT CCCTGACGAA ATTACCTCAA
    CAAGCTGGAA ATACAACTAT TGTCCATTCA CAAGAAAGAA AACAACAAAA TCTGTCACCA
    GAGATTGGAA AGCTATGCTT TACTCACTCT AAAAAAATAC CAAATTCTTC ACATTTTGTT
    AAGAAAGTGG ATTTCTTTAT TAGAAACAAT GAATGTAAAG AGGAAATTGA TTTCAGTAGG
    TATCATCCTG ATGATGAAGC TGATGAAATG AAGTCTTTCC TGGGAATATT TGATGACATT
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08610
    Clone ID Related Accession (Same CDS sequence) XM_005621938.2 , XM_005621938.1 , XM_005621939.2
    Accession Version XM_005621939.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GGAATGACTG CTTGCAGAAA ACCTGGGAAC
    CGAGAATGTA ATCATCACTG TAAAAATAAA CACACATGTG GACATGACTG CTGTAAAACT
    GGAGTGGCAC AAAAGTCAGA AACAAAAGAG CCAACAATCT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCACTTCCT CCAGTTAAAC GTCTCAAGAT GCAAATGAAT
    AAATCTCGAA GTGTGGACCT TAAAGAATTT GGTTTTACTC CAAAACCTTC TGTTCCCAGT
    ATATCAAGGT CAGAGTGTTT AAACATACCT GGATTGTCTG TAATGGAGGA GAGGGATCAG
    CGTGATTTCT GTGTAAAAGT TCAACAAGAA CCGTCTGAAT ATGAAGACAA AGATTTCTCT
    AGATGGAACT ATTCTGCAAC TTCAAATGAG AAATCCCCTC AAACAGTGCT GTTCAACAGA
    CATCTTAATG CATCTGGAAA CCAAAATTTG ACAGATGGGG ATAAAAGTAA TTCTCCATCC
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAGAACTTAT TAGAAGTTAC TAGTTTTTCA GCCAGTACTG AGAAGACAAA AACATCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTACCAGG GGAAGTAAGC TATTGCTCCA AGAGCCAAAG
    AGCAAATTCC AAGGAGAAAT GACAAAAAGT TTTGTTTCAT CACATGGGAA GCCAGATACT
    TATTTATCAA ATTCTCCTGT ATTCGAGTTT GATACATCCT CCCTGACGAA ATTACCTCAA
    CAAGCTGGAA ATACAACTAT TGTCCATTCA CAAGAAAGAA AACAACAAAA TCTGTCACCA
    GAGATTGGAA AGCTATGCTT TACTCACTCT AAAAAAATAC CAAATTCTTC ACATTTTGTT
    AAGAAAGTGG ATTTCTTTAT TAGAAACAAT GAATGTAAAG AGGAAATTGA TTTCAGTAGG
    TATCATCCTG ATGATGAAGC TGATGAAATG AAGTCTTTCC TGGGAATATT TGATGACATT
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621996.1
    CDS159..4604
    Translation

    Target ORF information:

    RefSeq Version XM_005621939.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HFM1, ATP-dependent DNA helicase homolog (HFM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621939.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGAAAT CAAATGACTC CTTGTTTTCT TTGGATACTT TGTTTTTTGA AAAACCAGAT 
    GAAGCTGAAA ACTATCCAGA CAATGAAAAA TTATTGGATT GGTTTCTCCC ACCTGTTCCA
    TTGATTTCAG AAATTCCAGA TACTCAGGCA TTAGAGGAGG AAATAGAAAG TCATAAACTG
    TTAGGTCAGG AAGAAAGGCC AAAAATGTTA CCATCAAATT TAAAGATGAC TAATGAAAAC
    ACAAATTATA TTTCACCAAC ACAAAAATTC CAATTTGCCT TTTCCTCAAA TAAATGTGAA
    ACAGGTGATC TGAATTTAAC AGAAGCAGGT AACCATGACT TACCACGTGT TCCTGGGAAG
    CTGACCTATG GTTCTCAGAA ATACAAAAAT CACATTGGCA CCGAGATAGC ACCTGAGAAA
    AATGTTTCAG GTAATACAAA ATTAGTAAAT TTTGCAGAAG ATAAAGGAGA GAGCACATCA
    GCCTTCCGAA AAAGATTATT TAAAATATCT GACAATATAC ATGAGGGTGT TTATTCTAAT
    GACAGTACAA ACTTGGAGTC TTCTGTTGGC TCAGTGAAAA TTGTCCCAAC AGAAATGAAC
    AAAGGGGAAT CATGGAAATG TAGCAATAGT AAGCAGAAAC ATCAATATTC AGACATTAAT
    ATGTTTACAG CAAGCAATGC TGTTTCAGCT TCTGAAATCA GAGAAGACAT ATTCAAAGGA
    CCAACTTTTT CAGTTGCATC CCAACCTCAT GAAGTTCAAG GGGTAACAGA AAATGATTTA
    GATTCTTTGA AGGCTGTCAC AGAAATCCCT GCAAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ACTATATACA GTCCAAAGCT TTTGATGATC TTCTTTACAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTGG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATAA AAATTGTTTA TATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTCGAT GATTGGAAAG AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATCCAACAT
    GCCCATATTA TTATGACAAC TCCCGAAAAA TGGGATAGCA TGACTAGGAA ATGGAGAGAC
    AACTCTTTGG TCCAGCTGGT TAGACTCTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGGG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT ACAGTCTTTA
    TCTCCTCCTT CAGAAAATAG TACAGTTATT CCAATGAGAT TTGTAGCTGT GTCTGCAACG
    ATTCCAAATG CTGAGGATAT TGCAGAATGG CTTTCAGATG GTGAAAGACC TGCAGTATGT
    TTGAAAATGG ATGAGAAACA TAGGCCAGTG AAACTTCAGA AAGTGGTCCT TGGATTCCCC
    TGCGGAAGTA ACCAAACTGA ATTTAAGTTT GATTTAACCC TCAACTACAA AATTGCTGGT
    GTTATACGAA CATACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCTGT TCTAGTGAAA GATGCCAAAT TTATTATGGC TGTGGAACAG
    AAACAGAGGT TACAGAAGTA TGCATATTCC ATAAGAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAGCTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTCA CTGTTGGAGA TCTACCAGTT CTTTTTACTA CCAGTACTTT AGCTATGGGA
    GTAAATTTGC CTGCTCACCT AGTAGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ATAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGATACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GTACAAAAGA GAAGTATATT
    CAGATGTTAG CTTGTAGTGA CACTGTAGAA AGCAGTTTAC ACAGACATCT TATCGAACAT
    TTAAATGCAG AGATAGTGTT ACATACCATC ACAGATGTGA ATATTGCTTT GGAATGGATA
    CGATCAACTT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAAGCAAAA TTACAAGAAT TATGTTTGAA GAATCTGCAT
    GATTTATCAT CTCTGGACTT GATAAGGATG GATGAAGATG TTAATTTCAA ACCTACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTACATT ACATTTGAGA CAGTGAAGAA ATTTTGTACT
    ATCAGTGGAA AAGAGACTTT ATTAGATCTG GTCACGATGA TATCCAGCTG CAAGGAATTT
    GTAGATGTGC AGTTAAGGAT AAATGAAAAG AAAATATTGA ATACTTTGAA CAAAGATCCA
    AATAGAGTAA CTATCAGATT TCCAATGGAA GGAAGAATTA AAACAAGAGA AATGAAAATA
    AATTGTCTTA TACAGGCTCA ACTAGGATGC ATTCCCATAC AAGACTTTGC TTTGACACAA
    GATATCTCGA AGATTTTTAG AAATGGCTCA CGAATTACAA GATGGTTGTC AGATTTTGTG
    GCTGTTCAAG AAAAGTTTGC TATATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGCAAACTTT GGGAAAACTC TCTACATGTA TCCAAACAGC TGGAAAAAAT AGGTATAACA
    TTGTCAAATG CAATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAACTGAT TTTAAATAGA CACCCCCCTT TTGGAACGCA AATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAAGTT GAAGTGGAAC AGATTGCAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACAATTATA TTAAGAAACT TTGAACAGCT ACAAGCTAGA
    AGAACATCAC CAGATTCTCA CTATGTTACC TTAATCATAG GTGATGCAGA TAACCAGCTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTATTAAAAG CTGGAAATTG GACTAAAAAG
    ATTGATGTGA GGAGAGCTTT TAAATCTGAA GATCTTAGCA TAAATCTAAT TAGTTCTGAG
    TATGTTGGAC TTGATATTCA GCAGAAATTT ACAGTCTTTT ACTTAGGACC CAGGAGGCTT
    GGAAATCAAA TAATTATGCA AAAAAAATCA GAAACAGAGA TTTCCCATTC TAAACATTCA
    AATAGATCTA CTATAGCAGG ACCCAATAAA GGAATGACTG CTTGCAGAAA ACCTGGGAAC
    CGAGAATGTA ATCATCACTG TAAAAATAAA CACACATGTG GACATGACTG CTGTAAAACT
    GGAGTGGCAC AAAAGTCAGA AACAAAAGAG CCAACAATCT CTTCATATTT ATCTGATTTA
    AGAAACAGGA ATGCTGTTTC ATCACTTCCT CCAGTTAAAC GTCTCAAGAT GCAAATGAAT
    AAATCTCGAA GTGTGGACCT TAAAGAATTT GGTTTTACTC CAAAACCTTC TGTTCCCAGT
    ATATCAAGGT CAGAGTGTTT AAACATACCT GGATTGTCTG TAATGGAGGA GAGGGATCAG
    CGTGATTTCT GTGTAAAAGT TCAACAAGAA CCGTCTGAAT ATGAAGACAA AGATTTCTCT
    AGATGGAACT ATTCTGCAAC TTCAAATGAG AAATCCCCTC AAACAGTGCT GTTCAACAGA
    CATCTTAATG CATCTGGAAA CCAAAATTTG ACAGATGGGG ATAAAAGTAA TTCTCCATCC
    TCAGAAGTTT TGAATGTGAA CTTTGAATTG GGAAATGAAG TTTGGGATGA TTTTGATGAT
    GAGAACTTAT TAGAAGTTAC TAGTTTTTCA GCCAGTACTG AGAAGACAAA AACATCAGGA
    TTTGGAAACA CTTTGAGTTC AAGTACCAGG GGAAGTAAGC TATTGCTCCA AGAGCCAAAG
    AGCAAATTCC AAGGAGAAAT GACAAAAAGT TTTGTTTCAT CACATGGGAA GCCAGATACT
    TATTTATCAA ATTCTCCTGT ATTCGAGTTT GATACATCCT CCCTGACGAA ATTACCTCAA
    CAAGCTGGAA ATACAACTAT TGTCCATTCA CAAGAAAGAA AACAACAAAA TCTGTCACCA
    GAGATTGGAA AGCTATGCTT TACTCACTCT AAAAAAATAC CAAATTCTTC ACATTTTGTT
    AAGAAAGTGG ATTTCTTTAT TAGAAACAAT GAATGTAAAG AGGAAATTGA TTTCAGTAGG
    TATCATCCTG ATGATGAAGC TGATGAAATG AAGTCTTTCC TGGGAATATT TGATGACATT
    TTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.